LOCUS       DAOSDA010000001        13905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_2979_length_13905_cov_3.735162, whole genome shotgun sequence.
ACCESSION   DAOSDA010000001 DAOSDA010000000
VERSION     DAOSDA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13905
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2979_length_13905_cov_3.735162"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..491
                     /locus_tag="PLW82_00005"
     CDS             234..491
                     /locus_tag="PLW82_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45693.1"
                     /translation="MPLTVPLHGFEPLYLVGEDEVELDGIVKTALFAQKEWIVDEYGY
                     EAEAELWLVPYTGEWKAVVKFYAPEEDFGWQMVLECVLLDE"
     gene            809..1342
                     /locus_tag="PLW82_00010"
     CDS             809..1342
                     /locus_tag="PLW82_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ45694.1"
                     /translation="MTEFLEVVKNRRSIRAFKDKPVEKEKIDQLLDCALRSPTGRAIR
                     PWEFIVVTDPDVLKRLANARGKGPNFLAGAPLGIVALADGTMTDLWIEDTSIAATVIQ
                     LTAEALGLGSCWMQIRDRKYGDTGSSEDYVREVLGIPEKYRVECIIAVGYKKEEKQPY
                     TDAEIEKLKARIHYNKF"
     gene            1665..2537
                     /locus_tag="PLW82_00015"
     CDS             1665..2537
                     /locus_tag="PLW82_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ45695.1"
                     /translation="MKDEIRLLQDEVSVLKNELADLTKTVNELLHLNKLVNLTKTVSE
                     LHDLVEKNAQAEQKAEPYDPEPKIKFGNVSGDAAEWRRVMSEQLIEQGYSPEEVERLL
                     TKIDREITDELKEDPEKLKALDGFRTEVTEMQRKFMQSCGSRFGVLVNTVEIKEPGKY
                     FIWGVLDNDEKFLSCDPMSMAKAFSVFSNEQRLLLLKALYENAELTAAELQEITGLAP
                     GGQFYHHLRELASVGLLAKSKRGSYALGATARTIVGTLLAIGNNLTSSTDNDMALAFN
                     MLHGMSRLDPEELD"
     gene            2857..3474
                     /locus_tag="PLW82_00020"
     CDS             2857..3474
                     /locus_tag="PLW82_00020"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HOJ45696.1"
                     /translation="MLVLYRLIEEYGYNEPILLKKVKITELSQDSVRQAFSRLASKGL
                     LERFSRGVYYVPTDTPFGKSRLNPKKVYEKMYIRDGENIFGYYTGLTFMNEIGLTTQV
                     PNLVEIVTNNDRSRGRIADIGYVKVRVRKSEVEVNKENVKTLQFLDLLRQVRVQDLSE
                     EARDLLKNYVRKQGFKKAEVFSLAKGFSSTVADRLIKSGLIDELT"
     gene            3461..4417
                     /locus_tag="PLW82_00025"
     CDS             3461..4417
                     /locus_tag="PLW82_00025"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOJ45697.1"
                     /translation="MNLHEDREKLNQAVLLVAETLKMHPAIVEKDYFVTLILKKIVEQ
                     EPHVVFKGGTSLSKCYKAIHRFSEDLDLSYDNGQQPVTQGMRRRCFKAVRTAIKNSNL
                     RFLNEGEPQSKRVFNKFLIDYGSTVQHELTALRPHVQVETSFQVPCFPIEIKEASSYI
                     YQYFEQERNIDVISQYGLEPFRVKVQTLERTFVDKVFAVCDYYLSDDISNHSRHLYDL
                     HKLFPEIETNQNLVGLIREVRSFRAKDSRCLSARGNCNINDILGEIIERSVYKNDYDE
                     MKRQLLFEDVPYNDAIETLRRIMNTSLFVTDQRAKTNKANLT"
     gene            complement(4527..5297)
                     /locus_tag="PLW82_00030"
     CDS             complement(4527..5297)
                     /locus_tag="PLW82_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOJ45698.1"
                     /translation="MPCSIAVIPAAGLGTRLRPLTYYIAKELLPLGTKPTLDYVMAEL
                     AECEIKDVVLVTSKSKKPHFEFYISETKRTYGISCHIALQESPLGLGHAVLCAKDVVG
                     NQQFAVVLPDDILVGENATMKLIEVSESQGGSAILSMRVDPDDVEKYGIIKTQSDGNF
                     VESLIEKPKKEEAPSNLAIVGRYCFSPLLWQKLDEGLERKLSTGDSKELDLTIPMNAL
                     IEDGHRIAAVEFGGTRYDTGNMKGYIRAFRELSDSTLD"
     gene            5496..6728
                     /locus_tag="PLW82_00035"
     CDS             5496..6728
                     /locus_tag="PLW82_00035"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45699.1"
                     /translation="MKPIRLAVVGGSSYYTPALIEVLNETDLEVDLVLNGRSEEKLSD
                     VAKVCRNLAKPSVRISTSTDLRESLAGADFILQQVRIGGMRMRAEDERFPFELGIIGE
                     ETIVPGGASLAARTIPYLKEFAGAVREVAPKARVLVLTNPNNMVIDYLNRYEGINAVG
                     FCDLPTSLLKGVVQAITGSAGDIETVWRDLSFKYYGINHLAFLGDVKYRGEMVDIVEI
                     APKLGLDPKLIETLELLPVSYLRYYYYTSTVLEEARKKPTRGELLYEVELDLAKQYRE
                     SQGEKPEALGRRQVPWYRDVVVRYINACREEGKAILVTTNQGAIPDLRDDQIAELPVN
                     VVRGEVQRIHQGKLPLQIRGLITSVADSERLIVDAVAQESANLFRQGLIAHPLVREVD
                     KAQRVMERIKEINDLNWL"
     gene            7088..8371
                     /locus_tag="PLW82_00040"
     CDS             7088..8371
                     /locus_tag="PLW82_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45700.1"
                     /translation="MRKSRILMVFVLALAIAGSAFAQGLNFKGFVENYSKWDKANGLQ
                     SQNNVELQMGLTAGGGDKVKAVVQIQPWKLNGTDVFGSTEDLTKNLSLAVSKMYIEAN
                     GPFWNGGPDMVTRLGDLDISYNPWVAEMSREGISISDLNLAGYDLGAFYLWDATGQAQ
                     GLKAQGYIGPVDSAVTVVNSDDETAVGLDAVVSPLKNLALKAALAANFEDDTDEMQKA
                     IKLDGAMQVTDKTLVNAGFRTVDADFDPVYRSHAKDQWGNPINPVDLYKGQTGFNVGV
                     QTEVKDVNLAASYDQPTALGKIGANTDIGGFRLAAGTELNVNGVTEISRRKTTASVEK
                     DFAGEKTAVTGRYEVAVEPAKAVKSKVSANAKTNLIESLPNLGLGASVEFDNFGENPV
                     AEVKADYTAPNGLTIKGGYNTAKGAVAEAGVRVNF"
     gene            9430..10542
                     /gene="ugpC"
                     /locus_tag="PLW82_00045"
     CDS             9430..10542
                     /gene="ugpC"
                     /locus_tag="PLW82_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOJ45701.1"
                     /translation="MANVVLEDVTKKFGNIIAVNEANLEIQDKEFVVFVGPSGCGKST
                     TLRMIAGLEEVTSGNIIIDDRDVTDVPPKDRDIAMVFQNYALYPHMNVYNNMAFGLKL
                     RKFPKEDIDKRVKQAAFMLGIEQLLDRKPKQLSGGQRQRVALGRAIVREPKVFLMDEP
                     LSNLDAKLRVQMRAELSKLHVRMQTTTIYVTHDQTEAMTMGDRIVVMRDGLIQQVGKP
                     LEIYDNPNNVFVAGFIGSPAMNFFDDCVLTYEDDEFWITSEIFKVRLPKEKAMKISVI
                     DQYVGKEIILGVRPEDIKDVNISPNMPEDSRFMAEVEVVEPMGSEVHLYLAQGDKLFV
                     ARVDAYTEAFEGDEIQVAFDMRKVHLFDRESEERLV"
     gene            10711..11376
                     /locus_tag="PLW82_00050"
     CDS             10711..11376
                     /locus_tag="PLW82_00050"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOJ45702.1"
                     /translation="MAVVNAYITRVVGLYGYLALFLGLAIEGTGMPGPVQIFFFTAGY
                     LIYQGKMSFLTAWIVTAVGNLVGNLVGYFIASLGGRPLVDKLFRMFGKDSSGLERYGK
                     KFSQKGPSIVFWGRLFGPIRTPAILAAGLFKYDLVQYTIFSALGAVIWSLFWLLLGLK
                     TAEGVDILKQFVPNSNYLIAGGIVLAVLGYFCWRYIKNRKQAKTSEVSEDETSSKLEV
                     EEQ"
     gene            11410..12462
                     /locus_tag="PLW82_00055"
     CDS             11410..12462
                     /locus_tag="PLW82_00055"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOJ45703.1"
                     /translation="MFTPDIVKKYDPTGMLTLIEQFPEQVQEAVAIGKKADLGNLPEE
                     VKDINSIIFLGMGGSAIGGDCLRSVLAGELRVPMQVSREYHLPAYVSEKTLVIASSYS
                     GNTEETISALREAEDRKAKILCISTGGKLADIATEKGYPLITIPGGISPRAALGYSFF
                     PILVAFSRLGLISVKKEAVAETIDVLEKMRLELSLATPGNPAQVLAQNLHGRIPLIYG
                     AEGYRGVAASRWKGQINENSKHVAYWNAFAELNHNEIVGWENPELTKEIHLVVLRDRE
                     DVRMAKRIEVTEEIVRSRVHGITEVHSRGEHDITRMFSLIYFGDFVSWYLAILNSADP
                     TPVRVIDYLKGELAKL"
     gene            13361..13696
                     /locus_tag="PLW82_00060"
                     /pseudo
     CDS             13361..13696
                     /locus_tag="PLW82_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014094092.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
BASE COUNT         4138 a         2772 c         3449 g         3546 t
ORIGIN      
        1 aatattgcgt atatcagata agcttattga taactgagga atttacacac taatttggac
       61 ttgatcccca aaacttaggc gatgttttgg cgtttgaatt tattgttgat cagttcggcg
      121 agtaccaaac tacgctgaaa gcattaaaaa gagataatgc ttatttcttt gttgacccag
      181 aggacaatga gcaattcgtt ctacagctac ctgtgcaacc aggagatctc gtgatgccac
      241 ttactgttcc gcttcatggc tttgaaccgc tatatcttgt cggtgaagac gaagtagaac
      301 ttgatggaat tgtaaaaacc gctttgtttg cccaaaaaga gtggattgtt gatgagtatg
      361 gttatgaagc ggaagcagaa ctatggcttg taccttatac aggagagtgg aaggcagttg
      421 tcaagtttta tgcaccagaa gaggacttcg gttggcaaat ggttctggaa tgcgttctac
      481 tagatgagta gcaatttctt ccatagattt tgatgaaaga ggtagagtaa acgagaggaa
      541 cgcatttttt ggaaagaatc gaagagcaaa tcctcgaagt tgatctccac ttgatatcct
      601 gttggtgtag aacatatttt aacgattgtg tcgctatcta aagtacccct aagcttgctt
      661 caaaggcaag tcataggggt attttgacgc tttgttatga gtaggactcg ctttgtgcga
      721 aatcggatag atttggaacc taaaacgagg gtaaactcgg ggcaatgtag tacagtctat
      781 gtagctgagt tttgaaggag ggatttctgt gaccgaattc ttggaagtgg taaaaaaccg
      841 cagaagcatt agagcgttca aggataagcc ggttgaaaaa gaaaagatcg accagctact
      901 tgattgtgcg ctaagatctc cgactggaag agctatcaga ccttgggagt ttatcgttgt
      961 aacagatccg gatgtcttga agagacttgc caatgcaaga gggaaaggac ccaacttctt
     1021 agcaggagcc cctctgggga ttgtagcgct tgcagatggt actatgactg atctgtggat
     1081 agaagacaca agcatcgcgg caactgttat ccagctgacg gcagaagccc tgggtcttgg
     1141 ttcatgctgg atgcagatca gggataggaa gtatggcgat actggttcca gcgaagacta
     1201 cgtcagagag gttctgggca ttcccgagaa gtacagggta gagtgcatca ttgctgtagg
     1261 ttacaaaaaa gaagaaaagc agccatacac tgatgcagag attgaaaaac tcaaagctag
     1321 aatccactac aacaagtttt agaaacctaa gcatgtctgc aaatagggtc accaattgta
     1381 gatagttgcg gaaaagaggc tgcgacttta aaaatacgta gagctgagat aggtcgatac
     1441 gttttaggat taaaggggag ctgttcagct cccctaagtt gttagtagct acacaaaaac
     1501 tattactggt tttctgtaca taggcatcca gatttgacaa ataaccacgg ttttttctct
     1561 gcttaaaaac tattttgtac aaaacacttg actattatac tgaagtacag taatatgtag
     1621 ttacggatac tagtaaccgt agcaatgtca tggaggtgtt cagtatgaaa gacgaaatcc
     1681 gtttgctcca ggatgaggta tctgtactta agaatgaact agcagacctg actaaaacgg
     1741 taaatgaact acttcaccta aacaaactag taaacctaac caagacggta agtgaactgc
     1801 acgacctagt cgaaaagaat gcccaagctg agcagaaggc agaaccctac gatccggagc
     1861 ccaagatcaa atttggcaat gttagcggag atgcagcaga gtggagaagg gtgatgtcgg
     1921 aacagctgat agagcagggg tactcacctg aagaagtcga acgtttactg acaaagatcg
     1981 acagggagat cacagacgaa ctgaaggaag atccagaaaa actgaaagcc ttggatggct
     2041 tcagaacaga agtcaccgag atgcagagaa agttcatgca gagctgcggg tcgagatttg
     2101 gagtattggt gaacaccgtg gagattaaag agcctggcaa gtactttatc tggggagtat
     2161 tggacaatga tgagaagttt ctgagctgcg acccgatgag catggcaaag gcctttagtg
     2221 tgttctccaa tgaacagaga ctgctcctgc tcaaagcact gtatgagaac gcagaactca
     2281 cagcagctga gctccaagaa ataaccgggt tagcaccagg aggtcagttt tatcatcact
     2341 taagggaact agcctctgtt ggtcttctcg ccaaaagcaa gaggggcagc tatgccctcg
     2401 gagcaactgc gaggacgatc gtcgggactc tgcttgcaat cgggaacaat ttaacttcga
     2461 gtacagacaa tgacatggct ttagcattca atatgctgca cggcatgtct cggttggatc
     2521 cagaagagct ggactgaggg gaaattatca tagtagcacc aagcaaaggg gagccatctg
     2581 gctccccttt gttatgggtt attgcggacg atggttcccg agtgtttgtg gatggtttat
     2641 cgtttccaga gtttatacct atggacgaga gctcggttgg caactctcgt ctgcataatg
     2701 ggacaggatc cttgagttcg ttgattgctt ggagggttcc acaagatcat aagattgatc
     2761 gtttgtagca cgagtatatt gcataacata taatgtcaca aaaattgaca caaaaaatca
     2821 aaatatgtat catagaggtg aaagcgagtg aaagacatgt tagtactgta caggcttata
     2881 gaagagtatg gttacaacga gccgattttg ttaaaaaaag tgaagatcac ggaactaagc
     2941 caagatagtg tcagacaggc atttagtcgc ttggcaagca aaggactgct agaacgtttt
     3001 agcagagggg tttattatgt tcctacagat actccttttg gaaaatctcg gttgaacccg
     3061 aagaaagtct acgagaaaat gtatatcaga gatggcgaga atatcttcgg ctattacacg
     3121 ggactgactt tcatgaatga aattggtctt acaacacaag tgccaaactt ggttgagatt
     3181 gtcacgaaca atgaccgatc tagaggaaga attgcggaca taggttatgt gaaagtcaga
     3241 gtccggaaat cggaagtaga ggtaaacaaa gagaatgtta aaacgctgca gttcctggat
     3301 ttgctgaggc aagttcgtgt tcaagatctc tcagaggaag ccagagactt actcaagaat
     3361 tatgttagaa aacaaggatt taaaaaagca gaagtgtttt ctctcgctaa gggatttagc
     3421 tcaacagttg ctgatagact tatcaagagt ggcttgatcg atgaacttac atgaagacag
     3481 agagaaacta aatcaagctg tacttcttgt ggctgaaaca ttgaagatgc atccagcaat
     3541 tgttgaaaaa gattactttg tgacacttat tctaaaaaag atagtggaac aggaaccgca
     3601 tgttgtgttc aaaggaggaa cgtcactatc aaagtgttat aaagcgattc atcgtttttc
     3661 ggaagatctg gacctgagct atgacaatgg tcagcaacct gttacccaag gtatgaggag
     3721 aagatgcttc aaagcagtga gaacagccat taaaaacagt aatctccgat ttctaaacga
     3781 aggggagccc caaagcaaga gagtgttcaa caagtttttg attgattatg gttccaccgt
     3841 acagcatgaa ttgacagcct tgaggcctca tgtacaagtc gaaacttctt tccaggtccc
     3901 gtgcttcccg attgagatca aagaggcgag tagttatatt tatcagtact ttgaacaaga
     3961 gagaaacatc gacgttatct cacaatacgg tctagagccg ttcagagtca aagtgcaaac
     4021 gcttgaaagg acattcgttg acaaggtgtt tgccgtgtgc gattactatc tttctgatga
     4081 catcagtaat cactcgaggc atttgtacga cttgcataag ctgttccccg agatagagac
     4141 aaaccagaac ttggttggtt tgattcgtga ggtacggtcg tttagggcta aagacagccg
     4201 atgtctttct gctcggggta attgtaatat aaatgacatt ttgggtgaaa taatcgaaag
     4261 aagcgtgtac aagaacgatt atgatgagat gaaacgacag ttgttgtttg aagatgttcc
     4321 ttataatgat gctatagaaa ctttgagaag aatcatgaac acaagcttgt tcgttacaga
     4381 tcaaagagct aaaactaaca aagcaaacct aacctgatag ataacgggga gcccgatggc
     4441 tccccgttta tcaaacagtt ttgctcgctc acaagcgtaa tcttaggtac agatggacgt
     4501 gttgtgcagg ggttacccct gaacccttag tccaaagtcg agtccgaaag ttcccgaaaa
     4561 gccctaatgt accctttcat gttgccagta tcatatcttg tgccgccaaa ctcaacagca
     4621 gcaattcggt gtccgtcttc gatcaacgcg ttcatcggta tagtgaggtc tagctccttg
     4681 gaatctcccg ttgaaagttt ccgttccaga ccttcatcga gcttctgcca gaggagagga
     4741 gagaagcagt accttcctac gatggccaag ttggaagggg cctcctcttt tttcggcttt
     4801 tcgattaagc tttctacgaa attaccgtcg ctttgggttt tgatgatccc atacttctcc
     4861 acatcgtctg gatctaccct catgctcaag atggcagatc ctccctgaga ttctgagacc
     4921 tctatcagct tcatggttgc gttttctcca actaaaatgt cgtctggaag aacaactgcg
     4981 aactgctggt tgccaacaac atccttcgcg caaagcacag cgtgcccaag ccccaaaggg
     5041 ctttcttgca aagcaatgtg gcaggagatg ccatatgttc tcttggtctc gctgatgtag
     5101 aactcgaagt ggggcttttt gctttttgat gttactaaaa caacgtcttt gatctcacat
     5161 tctgcgagct cagccatgac ataatccaag gttggtttcg tccctagagg caggagttct
     5221 ttggcgatgt agtatgttaa aggtcgcaat ctggttccca aaccagctgc aggtataacg
     5281 gcgattgagc aaggcataga agacaccact ctttcacaca gtttggaaca attatcaagt
     5341 tcaagagaga agggaaaaaa cccttgtttc tcaggcggca catgaaggag cagggtttta
     5401 cctcggttcc caggaaatat ctattagtct atatgagttc tagagagagt tcttatcata
     5461 aagttacaat caagcaaaaa ggcagggata aagccatgaa acctattaga cttgcagttg
     5521 ttggtggtag cagctactac accccggcat tgatagaagt cttaaatgag accgacctgg
     5581 aagttgattt ggtcctgaac ggcaggagtg aagaaaagct ctcagacgta gcgaaggttt
     5641 gccgaaacct tgcaaagccc agcgtccgta tctctacttc aacagaccta agagagagct
     5701 tggcaggagc agatttcatc ctccagcagg ttagaatcgg cggcatgaga atgcgagccg
     5761 aagatgagag gtttcctttc gagctcggta ttattggaga agagactatt gttcccggag
     5821 gggcatctct tgctgcaaga accattccgt atctaaagga gtttgccggg gctgtgagag
     5881 aggttgctcc gaaagcacgg gtgcttgttc taacaaatcc taacaatatg gtaatagatt
     5941 atctgaatcg atatgaaggg ataaacgcag taggcttctg cgatcttcct acatccctcc
     6001 ttaaaggtgt ggtccaagcc ataactggga gcgccggtga tatcgagaca gtttggagag
     6061 atctaagctt caagtactac ggcatcaacc acctggcgtt tttgggggat gttaagtatc
     6121 ggggcgaaat ggtggatatc gttgagattg caccaaagct cggtcttgac cctaagctca
     6181 tcgaaactct agagctttta ccggtttcgt acctccgcta ctattactac acctcaacag
     6241 tacttgaaga agccaggaag aagccgaccc ggggagagct cctctatgaa gttgagctgg
     6301 atctagcaaa gcagtaccga gaaagccagg gagaaaagcc ggaagcgctt ggcaggaggc
     6361 aggtgccctg gtacagagat gttgtggttc gctatattaa cgcatgtagg gaagagggaa
     6421 aagcgattct ggtcacaaca aaccagggtg caatccctga ccttagagac gatcagattg
     6481 cggaacttcc ggtaaatgtt gtaaggggag aggtacagag gattcaccaa ggaaagctcc
     6541 ccctgcagat aagagggctg attacctcag ttgcagacag cgaaagactg atagtagacg
     6601 ctgtggcaca ggagtctgcg aacctgttta gacagggctt gatagcgcat cctctggtaa
     6661 gagaagtgga taaagcccag agggtaatgg agagaataaa ggagatcaac gatttgaact
     6721 ggttgtagag cggtcgctgc accgctcttt tttatgcctc tgacttggca gcagctacca
     6781 atacctccta tatgtaccta aaggaaatgg catggaatca gcaaaatcta ataagcgtca
     6841 agagacacga aaggcacttc aatctggcaa ggaaaagagg tgcgtccccg ctgccgcaaa
     6901 ggtgcttaaa gtgatcttga cttaacggcg caaactttga actaaaaaac aaaaagagag
     6961 aggaaacaag gatgctcgcc tctttttgac acaacgcaga cttctataaa gtagggttcc
     7021 gagcaaaaga cgcactcaaa gctataaatc aaactcatct tatttcatta gggggactta
     7081 ttcatatatg cgtaaatcta gaatactaat ggtttttgtt cttgctttag cgattgctgg
     7141 ttcagctttt gcacaaggac ttaacttcaa aggctttgta gagaactact caaagtggga
     7201 taaagcaaac ggtctgcagt ctcaaaataa cgtagagctg cagatgggtc tgactgccgg
     7261 aggcggcgac aaggttaaag cagttgtgca gattcaacct tggaagctga acggtacaga
     7321 tgttttcggc agtacagaag acctgacgaa gaatctgtct cttgcagtaa gcaagatgta
     7381 tatcgaagct aacggtccat tctggaacgg cggtcctgac atggttactc gccttggcga
     7441 tttggatatc agctacaacc catgggttgc cgaaatgtcc agagaaggta tctccatcag
     7501 cgacctgaat cttgcaggtt acgacctcgg cgcattctac ctctgggatg ctactggtca
     7561 agcacaaggt cttaaagctc aaggatatat cggtcctgta gatagtgcag taacagttgt
     7621 aaatagcgac gatgaaactg cagttggttt ggacgcagta gtatctccgc tgaagaacct
     7681 ggccctcaaa gcagcgctgg ctgcaaactt cgaagacgat acagatgaaa tgcagaaggc
     7741 tatcaagctt gacggtgcaa tgcaggtaac tgacaagact cttgtaaacg ctggttttag
     7801 aactgtagac gctgactttg atcctgtata ccgcagccac gccaaagacc agtggggtaa
     7861 cccaatcaac cctgtagacc tttacaaagg tcaaactggt ttcaatgtag gtgtacagac
     7921 tgaagtaaaa gacgttaacc ttgcagcatc atacgatcag ccaaccgctc tcggtaagat
     7981 cggtgcaaac accgacatcg gcggtttcag attggctgca ggtacagaac tgaacgttaa
     8041 cggtgttacg gaaatttccc ggagaaagac aacagcttct gttgaaaaag acttcgcagg
     8101 cgaaaagacc gcagttaccg gtcgctacga agttgcagta gaacctgcta aagcagttaa
     8161 gagtaaggtt tctgcaaatg ctaagacaaa cctcatcgag agcctgccaa acttaggtct
     8221 tggcgcttcc gtagagtttg acaacttcgg cgaaaaccca gttgcagagg tcaaagccga
     8281 ctacacagct cctaacggcc tcaccatcaa aggtggatac aacacagcta aaggtgctgt
     8341 tgcagaagca ggcgtaagag taaacttcta attgagtcat agagttggag aatagagaga
     8401 aaaggcaaag cctccgtcac ccggaggctt tgctggtata aaaagcttta gacgtcgtaa
     8461 ttcttggatg ttggaaaaca ggacataggt tgtagtgtgt cacgactctg tcaaatgagt
     8521 aaaaagcatt aggacatatt tcggaaagag gcgtaaagtg tagcgataag aataaagaaa
     8581 ggagtcctga agttggcaga gattgaagga cgcagaaggt gcgacagcgc catcttcatt
     8641 cttttcatta tccttattct gcttgtcctg tttgaggacg atttcctgtt ttagtttctt
     8701 acttagaggt agcctgctcc gctacctctt ttgcttatga tacaaatcct gtaaggcgga
     8761 taatatggtc gttgcagttg ctcacgaacc aagccgagct ggagcggaga gttttaagtg
     8821 ttacatgggt attagaataa gtcgtggttt actgctggtt tgagatggat ttctggaaga
     8881 tatgtgccct gaaaaaattt gttggcattt tgctactcct gcagcggaca agccattctc
     8941 cggtagatgc tgcgctgcag gaaacgctga acttgattgt tgaatttaaa atgaattaat
     9001 agtaatattg agttaagttt taaatttatt tagaaaaaca acctgacctg gaataagtgg
     9061 cagtgttcca gatatttaaa agaggagttg ggtcctgtta ggcgaacttt ctattggtaa
     9121 aaagtcatat ggttttgagc caagaattgg agagataatt ataaaatatc ccaactgtag
     9181 gcggaaagaa gcgttttttt gcaaaaacta ggcttggaat gcaggataac gcttccactc
     9241 gttgaataaa aaagtaaggg aataggagtc ttttttgttc ctgggaggta aatccccaag
     9301 actaagcctt aggcctggca taaaaggcct ttttatgggg tgcttctagg tttttgggat
     9361 tccaactttg gaagaatcat tattttttta acacaatgtt tccaactgac acatgaggag
     9421 gagaatatag tggccaacgt tgtattggag gatgtaacca agaaatttgg taacatcatc
     9481 gcagttaacg aagctaacct ggaaatccag gataaggagt ttgtagtttt tgtagggcca
     9541 tctggttgcg gtaagtccac aactttgaga atgattgcag gtctcgagga agtcacatcc
     9601 ggcaacatca ttatcgacga tagggatgtt acagacgttc cgcccaaaga cagagacatc
     9661 gcaatggttt tccaaaacta cgctctgtat ccgcacatga acgtttacaa caacatggca
     9721 ttcggtctca agctgcgtaa gttccctaag gaagatatcg acaagagagt taagcaagct
     9781 gctttcatgt taggtattga acagctgctc gatcgcaaac ccaaacagct atctggtggt
     9841 cagagacaac gtgttgctct cggccgtgct atcgtccgcg aaccaaaggt tttccttatg
     9901 gacgaacctc tctccaacct tgacgcaaag ctccgcgttc agatgagagc cgagctcagc
     9961 aagctccacg tccgtatgca gacaacaacc atctacgtaa ctcacgacca gacagaagct
    10021 atgaccatgg gcgaccgcat cgttgttatg cgcgacggtc tgattcagca ggttggtaaa
    10081 cctcttgaaa tctacgataa ccccaacaac gtattcgttg ctggatttat cggcagccct
    10141 gcaatgaact tctttgatga ctgcgtcctc acatacgaag acgatgagtt ctggattacc
    10201 tctgagatct tcaaagttcg cctgccaaaa gaaaaggcaa tgaagatcag cgttatcgat
    10261 caatacgtag gcaaagagat cattctcggc gtacgcccag aggatatcaa ggacgtcaat
    10321 atcagtccta acatgcctga agactctaga ttcatggcag aggtcgaagt tgttgagccc
    10381 atgggttcag aagttcacct ctatctggca caaggcgaca aactcttcgt tgcccgcgta
    10441 gatgcttaca ccgaagcttt cgaaggcgac gagatccaag tagctttcga catgagaaag
    10501 gtacacctct tcgatcgcga atcagaagag cgtctcgttt aatcagacaa tttgggctac
    10561 cggcatgagt cggtagcctt ttttctttgc agctagatac ttcctactat ttcaggaagg
    10621 atccggcaaa caaactttac aacgaagcca ctttgaggta tcctgtacat agatgtgatt
    10681 tgaccttaat tcgattggta ggggataccc ttggctgtag taaacgcata tataacgaga
    10741 gtagtcggtc tgtacggcta cctggcctta tttttaggct tggctataga aggtacagga
    10801 atgccgggac cggttcaaat atttttcttt acagcaggct acctgatcta ccaggggaaa
    10861 atgtcctttc tcactgcttg gatcgttaca gctgtgggga atctggtagg gaaccttgta
    10921 ggttacttca tcgcgagctt aggcggtagg cctttagtgg ataagctttt ccgaatgttt
    10981 ggcaaagact caagcgggct tgagcgttac ggaaagaagt tttcccaaaa gggtcccagc
    11041 atagtgtttt ggggacgcct gtttggtcca atcaggactc ctgcaattct tgctgcaggg
    11101 ctttttaagt acgaccttgt gcaatacacg atcttcagtg ctttaggcgc agttatctgg
    11161 tctttgttct ggctgttgct gggattaaag accgcagaag gtgtagacat actgaaacaa
    11221 tttgtgccaa acagcaatta cctaatcgcc ggcggtattg tgctcgcagt tctagggtac
    11281 ttctgctgga gatatattaa gaatagaaag caagctaaaa cttccgaagt gtctgaggat
    11341 gaaaccagtt cgaaactaga ggtggaagaa caataagaga ttctgtgcag ctcgaaggag
    11401 gcatttttga tgttcactcc agatatagtt aagaagtacg acccgactgg aatgcttacc
    11461 cttatcgaac agttcccgga gcaggttcaa gaagcagttg caatcggaaa aaaggctgat
    11521 ttgggcaacc tgccagaaga ggttaaggac ataaactcca tcatcttttt gggtatgggt
    11581 ggctcagcca tcggcggaga ttgtttgaga tcggttttgg ccggagagct gagagtacct
    11641 atgcaggtaa gtagggagta tcaccttccc gcttacgtca gcgagaagac tttagtgatt
    11701 gcatctagct attcgggaaa caccgaagag actatttctg ccttaagaga ggctgaagat
    11761 agaaaggcaa agattctatg catttcaact ggtgggaaac ttgccgacat tgcaaccgaa
    11821 aagggttacc cactgataac cattccgggc ggcatctctc ctagagctgc gctcggctac
    11881 tctttcttcc cgattctagt ggctttcagc cgtttagggc ttatctctgt caagaaagaa
    11941 gcggttgcag aaaccataga tgtgttggag aaaatgaggc ttgagctgtc tcttgccact
    12001 ccagggaatc ccgcacaggt cctagcccag aacctccatg gtagaatacc cctgatctac
    12061 ggtgccgaag gctaccgcgg ggttgctgct agtaggtgga agggtcagat taacgaaaac
    12121 agcaagcatg tagcctactg gaatgccttt gcagaactca accacaacga gatcgtaggt
    12181 tgggaaaacc ccgagctgac aaaagagatt cacctggtag tgctcaggga tagagaagat
    12241 gtacgtatgg caaagcgcat cgaagttacc gaggagatcg taagatctcg cgttcacggc
    12301 ataaccgaag ttcattcaag aggagagcac gacatcacca gaatgttttc cctgatctac
    12361 ttcggcgact ttgtcagttg gtatttggca atactgaact ccgccgatcc aactcctgtt
    12421 cgagtgatag attacttgaa aggcgaactc gcgaagctgt aaaagatctg tttgcatcaa
    12481 gaacgttcat catggcggga ccgggaggtc ccgccatttt tcaggtgctg catttgttag
    12541 aaagttaacg aattgacttt tctcactgtc aagttgacat ttgtaaggtt taacattgtt
    12601 tgacaaacgt ttttatgtta tttttgtata gtattgaaga ataaagaacg gaaatataca
    12661 cgcccttcgt aaaatgattg acaaacagaa atggcaacat aggttttcct ggcaacagaa
    12721 ataattgttt agatcggttt tcatttggat aataagttat ttgttcaggg ttggaaccaa
    12781 aagccggtgg agatgaaagt gggaacattc ccaagctgat ttataaaaat acagagaaat
    12841 caaccctaat cgcttgagag aaacccacag tgcgtttata agaaacgtgc gatgtgcgat
    12901 atacatacta gagttaggcg gactgttcta gcggttgggt gtaggcatac aatggtagta
    12961 gtgcgggttc taatttctga atacttaaac tgtacaacga ccgctatata acaatgtcat
    13021 cggagttcag agttaaaaaa actgaaagat gtctagctaa gtttactatt tgcgcatatt
    13081 tgcaataatc gatccatgct ttgggtttta taatataaag ctgcgttata gaatagtagg
    13141 ggggtggaaa tgtctaaaga attgtgatag taagaaaacc agttttcgaa cacttttagg
    13201 aaacaggcaa ctaaccaaat tgggtaggcg gttttaacac aaaaactcga atggcggtat
    13261 aggaatgagt gtaaactact tagaacaacc aaaaaaagca aataatagct gtaaaggggg
    13321 gaaaaaggta gtggcgaaga aaaagcagaa gactccgaga atcaggaggg gagagatcta
    13381 ctttgctaat ctgaaccctg ttgtcggatc tgagcagggg ggcgtaaggc cagttcttat
    13441 cctgcaaaac gacacaggca acaagtacag tccaacgacg attatcgcag ctgtaacatc
    13501 aaaacataag agatcaagac tgcctacgca tgtgcccctt gcttctagag agggacctct
    13561 tgacaaagac tcggtagtac ttctagaaca ggtgcgtaca attgacaaga gcagactgaa
    13621 ggaaaagatc ggtaagctat caaaggaaga gttagaacag gtccagaaag ctctcaaact
    13681 ttcccttggt tttgatgacc cacataacta caattcagat gagacagctg aggaagatga
    13741 ggaagaggaa gacaaaaaaa tcctgcattg attgcactcc cgcttctgga tttctttaca
    13801 tggaagggat tgaccatctt gaagccaatt ataggtatag catgtggtca gaaatggcaa
    13861 ggaaacaggc agactttgta tgtacataag ccttattctg acgca
//
LOCUS       DAOSDA010000002        12887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_3280_length_12887_cov_3.780003, whole genome shotgun sequence.
ACCESSION   DAOSDA010000002 DAOSDA010000000
VERSION     DAOSDA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12887
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3280_length_12887_cov_3.780003"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..135
                     /locus_tag="PLW82_00065"
     CDS             4..135
                     /locus_tag="PLW82_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HOJ45704.1"
                     /translation="MKRRLVEANIKKDSAIIGEVVDLISNLRETWQEAMKTGQGSNG"
     gene            128..559
                     /locus_tag="PLW82_00070"
     CDS             128..559
                     /locus_tag="PLW82_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45705.1"
                     /translation="MDDLVKKTSVLLAEKRDLLKEIVELCSKELQAALEDKWAEFNSL
                     AENFKTVSEKIDKLDILIGELIAGQEIQSRKLEELDKEIQTLVLECLELQKKTANVVE
                     QKKAHALSQLKVLETRNDAVKAYNRPSSYHKSIPKFVDDKK"
     gene            731..931
                     /gene="cspD"
                     /locus_tag="PLW82_00075"
     CDS             731..931
                     /gene="cspD"
                     /locus_tag="PLW82_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein CspD"
                     /protein_id="HOJ45706.1"
                     /translation="MLGKVKWFSNEKGFGFIEPAGGGADVFVHYTAIVGDGYKTLEEG
                     QEVEFEIVEGARGPQAANVQKA"
     gene            1059..1625
                     /gene="raiA"
                     /locus_tag="PLW82_00080"
     CDS             1059..1625
                     /gene="raiA"
                     /locus_tag="PLW82_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOJ45707.1"
                     /translation="MKIAVLGKNIGITDALRSYVEKRVGRLERYLQGFGKDNIDCTAV
                     LSVERSMHVTEVTIDLGAMTMRGEERTADMYESIDGVVEKLERQIRKYKTRIRNKNRQ
                     ETRDAISEINRKLLEVPNGYDDTHQEIKIVRRKRFVIKPMSVEEAVMQMDLLGHDFFV
                     FTDAEDNEVKVVYKRKDGNYGLIEPEIG"
     gene            1857..4610
                     /gene="secA"
                     /locus_tag="PLW82_00085"
     CDS             1857..4610
                     /gene="secA"
                     /locus_tag="PLW82_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOJ45708.1"
                     /translation="MIKFIKSLFDPEKKELARLAEITKLVNAREAELKSLSDDELRAK
                     TEEFRKRLADGQTLDDILPEAFAVVREASVRTLGMRHFDEQVMGGIALHEGKISEMKT
                     GEGKTLAATMPVYLNALEGKGVHVVTVNDYLARRDADWMGAIYRFLGMSVGCILHGQT
                     FEERKKAYACDITYGTNNEYGFDYLRDNMVLRAEDMVQRDLHYAIIDEVDSILIDEAR
                     TPLIISGSGEESTELYSKFASIVPRLKHDEDFTIDEKAKTVAITENGVEKVEKILGVE
                     NLYDPENIGLTHHLNQALRAQFIMKRDVDYVVKDGEVIIVDEFTGRLMPGRRYSNGLH
                     QAIEAKENVKVAAENRTLASITFQNYFRMYKKLAGMTGTAKTEAEEFLKIYGLRVVEI
                     PTHKPMIRTDYPDVIYKTEVAKYKAVIEEIKEMYAKKRPVLVGTISIERSELLSELLK
                     RAGIPHKVLNAKNHEQEAEIVAQAGRIGAVTISTNMAGRGTDIILGGNPDFLVKERLR
                     KEGLSPEMVSAASEKVPSENLANSLGISVDELKELQSRYEEMLREAKKELEVEHQKVV
                     ELGGLHIIGTERHESRRIDNQLRGRAGRQGDPGSSRFYISLEDELMRRFGGDRALGLL
                     EKLGWTEDMPIEHDAISRQIESAQKRVEAWNFNIRKNVLEYDQVLNRQREAIYSLRRG
                     FLLGGQSRQTIDSMIENTVKNLVEYATVDTEQVAQWPWDSILSSFRELIGTYDQGVRD
                     LAAKLQQSHKNSSTRDALEDIVQQFTDEVKKAMAQREEEIGKALFQEISRVVTLRNVD
                     QAWMDHLDNMDDLKEGIGLRAYGQMNPLIEYQKESSRLFEATLMRIEEETIRLLFHLR
                     LANEEEVKRAEDREKAKLSALQSNRNAAEAQAAPVQAKKEPGRNDPCPCGSGKKYKKC
                     CGK"
     gene            4695..5729
                     /gene="prfB"
                     /locus_tag="PLW82_00090"
     CDS             4695..5729
                     /gene="prfB"
                     /locus_tag="PLW82_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156946446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOJ45709.1"
                     /translation="MSIFERSKVDERISALEQEVLAPNFWSDQRLAQKKTKELNELKE
                     SRDTLQKLARDLEDLKVLLDLAVSEEDGDTLNDVIAESERLLKFAERYELEMLLNGPY
                     DSSDALLTIHPGAGGTESQDWGSMLYRMYMRWAEINDYTAKVLDFQPGDEAGIKSVTI
                     SISGPYAYGYLKAEAGVHRLVRISPFDSAGRRHTSFCSVDVIPQISDAGEIEIDPKDL
                     EIDTYRSGGAGGQHVNKTDSAVRITHIPTGIVVQCQNERSQHANKETAMNMLMSKLVA
                     LKLQKQKEELEAIRGEKAENTWGSQIRSYVFCPYTMVKDHRTGLETGNVDAVMDGAIQ
                     DFIEAYLRMK"
     gene            5743..6444
                     /locus_tag="PLW82_00095"
     CDS             5743..6444
                     /locus_tag="PLW82_00095"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HOJ45710.1"
                     /translation="MKKQRKLGRWFLPSLTLVLLLLTIAFYLPVLVTDQLTKAIAVNL
                     YSFDSLSVKHKASPAFLLFFGKIRDLTIEGSNLEGQYINLSRVFLQVDKLKVSMWDLL
                     VYGRLNILDSEGRAALSISQAELDKYLHRFYIDDQYHSIRLDLLDDGIRISGTLDGRE
                     FYLDCVFTVEQNSVLALSPKRMVSTNGSNQNLMNRLGQMASYSIDLGVFGFPLIIDDA
                     SSRDGYLYVFASVSK"
     gene            6502..6981
                     /locus_tag="PLW82_00100"
     CDS             6502..6981
                     /locus_tag="PLW82_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOJ45711.1"
                     /translation="MKVLEKNTQNELQVVVFKIKDEMFAADIHQVKEIRGVDKVTKVP
                     DAPDVLEGIIHLRGQIYPIVNLSKRFRKDKYEITPQSRTIIIDLKGSRFGVIVDEVTE
                     VIRVKKEDIQSTPDILSSQNGYLEGVIKRDEQLILFVNLEKILTDKELNEINKTKDL"
     gene            6996..7727
                     /locus_tag="PLW82_00105"
     CDS             6996..7727
                     /locus_tag="PLW82_00105"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HOJ45712.1"
                     /translation="MAIKLDVNQRVQVSRTTSGDAKREYYPSRIEEITNDAIFIAEPL
                     DGTVPVYLRLGEEIEVFFNTEAGTFRFTSKVLQRLERNIRMVKISKPETFTKSNRRDF
                     FRLKVFIPFYFRVLPEATFNYGHPHKQEDLKKTILDKQLALDETGKQEGIVKDLSGGG
                     LLAAVSEKSGLKLGDHLEMWLPLDNEGVYVWGDVVRILHNPDATKYNVDVGIHFSKIE
                     EKVRDQIISHVLSLQRELLQKGALE"
     gene            7782..9704
                     /locus_tag="PLW82_00110"
     CDS             7782..9704
                     /locus_tag="PLW82_00110"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HOJ45713.1"
                     /translation="MKKGWIWIVVIGLLCSAFAIYQRVRIEQADRTVQLVLDSRSLAE
                     YAADKGIPLEALYAEVADLDAFDLAISEVKLWESVASRSTVVYPGFYLLFSSPEMEGT
                     DPRKLYIADDTSGPIAGRLSPLGVPFFADDARLWEFQEEYPVDVNRRKEALREPTGSL
                     WDEALYPDYSSLDLAQCFGMQVVARIQNPYVHTPESLKYAVDQWDGAGNLVIFEGKEV
                     LGYPGNLKSAAELLGDTPWGFIEFANQYGEKDLARLTNYNLIRVHSITPGEMLKIDPQ
                     VAMERYLRAVRERGARVLYLRPFPKLGLEENLAWFAGLEQNLKDDGYRLGQAEPKPFF
                     KSSFLIFLPVLLAVVICGIRLMLLLEFRETTSLFSGLFALVAVASVYLKGYTILARQI
                     TAFGAAVIFPTLAVGFVAKQIGEGKARLWQTMGTVLFYTAAGSMLLSAALCDLRFVIK
                     IDQFLGVKLMHIIPPLLTLWISIRSISRGAFGAAGFKSNIRATLSSLWPPRLGHIIVL
                     VLVLFAGFVYIGRTGHDWGLPVLELETKIRVFLEDLFVIRPRLKEIFIGHPALFLGLF
                     IASSVLIRRLKWLLPLILTVGSIGVTSVLNTFSHAHTPFLVSVVRTGWGFAIGMLLGL
                     VAFGLLRLIVRVENHG"
     gene            9697..10686
                     /gene="csaB"
                     /locus_tag="PLW82_00115"
     CDS             9697..10686
                     /gene="csaB"
                     /locus_tag="PLW82_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HOJ45714.1"
                     /translation="MGKVLVFGYYGFGNFGDELLVAAIKEHLQDHDLTVVSGNPKETE
                     KIHELKAISRKDFWKNPGSYDILLLGGGGLLQSVTSNRSLLYYLAAIRWARAKGLSVY
                     LMAQGLGPFKKGPIYPLVDLGMRSLKGLEISVRDKKSKDLAASFGLEADLVADLALTY
                     TGFPTRKTGEGTKRVIGIAPKAGFDVRQIRWLESFGDETTVRLFAFHKDDHRVCLEVE
                     KLLKDAHTIKMEKTQDLENFADIDFLWGMRLHSLIVAAKMGIPAIGISYDPKVEQFCR
                     EVGFAWHSPETMPKEEWIRSGNATELIQRAEKAWALFRSYAETQANRTPGHSH"
     gene            10589..11401
                     /locus_tag="PLW82_00120"
     CDS             10589..11401
                     /locus_tag="PLW82_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOJ45715.1"
                     /translation="MQQNSSNEQKKPGPCSEATPRPKQIELLDIPIDAVDFSGALAII
                     GRFIAEERKNPALVFTPNAEILVKAHDDAEFGRVLKNADLVVPDGTSLLWASKVLKRP
                     LETRVTGIDLMQRLLTEAENAGWSVYFLGAKPDVIVEAAARVKHLHPGLKLVGYHHGY
                     FDDDFSVIEGINKLRPDILFVGMGAPKQELWLTRNREELNCRVALGVGGSFDVLSGRV
                     DRAPLWMQKKGLEWLYRYIREPSRLKRSILLPRFVRLVLKEKFGTSKGETTQ"
     gene            11398..12258
                     /locus_tag="PLW82_00125"
     CDS             11398..12258
                     /locus_tag="PLW82_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOJ45716.1"
                     /translation="MTSRLFKEIMRMLGVLAGTTVLALSLVLFLIPNKIAPGGVSGLA
                     TVVYHLSGLSVGLVMLLINIPLFLLGIKKLGFVFGLRSLVATIYLSFAVDILQRYVSP
                     VTQDPLLAALYGGIIMGVGLGIAITNGGTTGGTDLAAVLVHRFLGLSVGATLLVIDAI
                     VIVIAGFFFSLELAMYSALALFVATKLLDLMQEGWHYARAAYIISDKSDDIAQAIMTK
                     MERGVTGFYGKGMYSGKDKNILYVICSRSEIATLKDLVRTIDKDAFIVIGEATEVLGE
                     GFRANKGTNE"
     gene            12275..>12887
                     /locus_tag="PLW82_00130"
     CDS             12275..>12887
                     /locus_tag="PLW82_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOJ45717.1"
                     /translation="MDDKKLEELKEIAKESRVWIIKSLAEAKSGHPGGSLSCIDILTY
                     LYFHAMNVDPTNPDDPDRDRFVLSKGHCAPALYTVLALKGFFPKEELLKLRKLGSILQ
                     GHPDAVKVPGVDASTGSLGQGLSIANGMALAGKLDRKDYTVYTIIGDGETEEGQIWEA
                     AMTAAHYQLGNLIAFLDYNHLQIDGRIEDVMGNGNFTNRFKAFG"
BASE COUNT         3647 a         2834 c         3343 g         3063 t
ORIGIN      
        1 tatatgaagc ggagattggt cgaagctaat attaagaaag actctgccat catcggcgag
       61 gttgtggatc tgatttccaa cctcagagag acctggcagg aggcaatgaa gacggggcag
      121 ggttccaatg gatgatctag taaaaaaaac ctccgtgctc ttggctgaaa aaagagatct
      181 tttaaaagaa atcgtcgagc tgtgcagcaa agagttgcag gcagcacttg aagacaagtg
      241 ggccgagttc aacagcctcg cagaaaattt taaaactgtg agtgaaaaaa tcgataagtt
      301 ggacatacta atcggggagc tgatagcagg ccaagagata cagagcagga agctggaaga
      361 gctggacaaa gaaatccaaa ccctagttct ggagtgtttg gaacttcaga agaagaccgc
      421 aaacgtagtt gagcagaaga aagctcacgc cctcagtcag ctcaaagtgc tggagacaag
      481 gaatgatgcg gtcaaagcct acaacagacc ttctagttac cacaagtcta ttcctaagtt
      541 tgttgatgac aaaaaataaa gtctgcagct tatttttgac ggtttaagac cgaaaaggcg
      601 gatgccggtg cattttgtgg agttatctag tgatgtcgcc ttcggttgac agaatataac
      661 atatgatata cgctagtaat gaaattgttt ttcttgggtt tccaagtaac taggaggttt
      721 tctcgaaaaa atgttaggaa aagtaaaatg gtttagcaac gagaagggtt ttgggtttat
      781 tgagccagct ggcggcggtg cagatgtttt cgtgcactac acagcaatcg tgggagatgg
      841 gtacaagaca ctagaagagg gacaagaagt cgaatttgaa atcgttgaag gcgctcgcgg
      901 accgcaggct gccaatgttc aaaaagcata acagtgtcta taaagaggtt gctcagcaac
      961 ctcttttttg gtgtttaaaa agccaagtcc tgggtaataa ataaatagac acaagaatat
     1021 gaagtagtac ccattttcct gtgaagggag taatgcggat gaagatcgct gttctgggaa
     1081 aaaacatcgg aattacagat gcattgagaa gctacgttga aaagagggtg ggtcggctag
     1141 agaggtactt acaaggcttt ggcaaagaca acatcgattg caccgctgtc ttgagtgttg
     1201 agcggagcat gcatgttacc gaagtcacca tcgatctcgg cgctatgact atgcgcggag
     1261 aagaaagaac agccgacatg tatgaatcca tcgatggcgt agtagaaaaa cttgaaagac
     1321 agattagaaa gtataagacc agaattagaa acaaaaacag acaggaaacc agagacgcca
     1381 tttccgaaat caaccgcaag ctcctagaag tccccaacgg ttatgacgat actcatcagg
     1441 agattaaaat tgttagacgc aaacgctttg tgataaaacc aatgtcagta gaagaagcag
     1501 tcatgcagat ggatctcctc ggacatgatt tctttgtctt tactgatgcg gaagataacg
     1561 aggttaaggt tgtttataag cgcaaagatg gcaactatgg tttgattgaa ccagagattg
     1621 gttagataga taaggccgga cgcagtccgg ctttttttgt tttataggca aaccttcttg
     1681 gggccttttc acaggtcaac atctgaacac tacgagaatt acttcatcta tatatatgat
     1741 gagaatccaa ctccctctgt gatataatat taacgtcgtt atactctggg agtttttccc
     1801 gaaaatcaca aggtgttgtt gggtatttag gtttgacaat cagggaggta tttgtcttga
     1861 ttaagtttat caagagtctt tttgacccag aaaaaaagga actggcccgg ttagctgaga
     1921 tcaccaaact ggtcaatgct agagaagcag aactcaagag cctgagcgat gatgagcttc
     1981 gggccaaaac tgaggagttc agaaagcgcc tcgcagatgg tcaaaccctg gatgacattt
     2041 tacctgaagc gtttgcagtt gtaagagaag catcagtgag aactttggga atgaggcact
     2101 ttgacgagca ggtcatgggc ggtattgctc ttcacgaggg aaagatctcc gaaatgaaaa
     2161 ccggtgaagg caagactctg gctgccacca tgccggtcta tcttaatgct ctcgagggta
     2221 aaggcgtcca cgtcgttacc gtcaacgact atcttgcccg cagagacgcc gactggatgg
     2281 gtgccatcta cagattctta ggaatgtccg taggctgtat tctccacgga cagactttcg
     2341 aagagagaaa aaaggcctac gcttgtgata taacctacgg aaccaacaac gagtacggct
     2401 tcgactatct cagagacaac atggtcctcc gcgcagagga catggtgcag agagaccttc
     2461 actatgccat catcgacgag gtcgacagca ttctcattga cgaagccaga acgccgctga
     2521 tcatttccgg aagcggcgag gaatctacag aactgtacag caagtttgca tcaatagtcc
     2581 ccagactgaa gcacgacgag gacttcacca tagatgaaaa ggccaagacc gtggccatta
     2641 ccgaaaatgg tgtggagaag gttgagaaga tcctgggcgt agaaaacctc tacgatcctg
     2701 agaatatcgg tcttacccac cacctgaacc aagctctaag agcccagttc atcatgaagc
     2761 gcgacgtaga ttatgttgtg aaagacggcg aggtaattat cgtcgacgaa ttcactggga
     2821 gactcatgcc cgggcgccgc tactccaacg gtctgcacca ggctattgag gctaaggaga
     2881 acgtaaaagt cgctgcagag aacaggacct tggcaagcat cactttccag aattacttcc
     2941 gcatgtacaa aaagcttgca ggtatgacag gtaccgcaaa gaccgaagcg gaggagttcc
     3001 tgaaaatcta cgggctcaga gtagtggaaa tcccaacgca caaaccgatg atccgtaccg
     3061 attaccccga cgtaatctac aagaccgaag ttgccaaata caaagcggtc atcgaagaga
     3121 taaaagagat gtatgccaaa aagaggccgg tcctggtggg aactatctcc attgagcgat
     3181 cagagctcct ttccgaactg ttgaaaaggg caggcattcc acacaaagtc ttgaacgcca
     3241 agaaccacga gcaggaagca gagatcgttg cccaagcagg ccgaatcggt gcagtaacca
     3301 tctctaccaa catggctggg cgcggtaccg atattatcct tggcggaaac cccgacttct
     3361 tggtcaaaga gaggcttaga aaagaaggtc tttcgccgga gatggtcagc gccgccagcg
     3421 aaaaggttcc cagcgagaat ttggcaaaca gcttaggcat cagcgttgat gagcttaaag
     3481 agctgcagag ccggtatgag gaaatgctcc gggaagctaa aaaggaactg gaagttgagc
     3541 accaaaaagt tgttgagctg ggcggtctcc acatcatcgg tacggaaagg cacgaaagcc
     3601 gcagaatcga caaccagctc agaggacgtg caggtagaca gggtgaccct ggatcttcca
     3661 gattctatat ctccctcgag gatgaactca tgagaagatt cggcggtgat cgggctctag
     3721 gtcttttgga gaagctgggc tggaccgaag atatgcccat cgagcacgac gccattagcc
     3781 gccagatcga gtcggcacaa aaacgcgtcg aagcgtggaa cttcaatatc cgtaagaacg
     3841 ttctcgagta cgaccaggtg ctgaataggc agagggaagc catctactcc cttagaaggg
     3901 gcttcctcct aggaggacag tcgcgccaga caatcgacag catgattgaa aacaccgtta
     3961 aaaacctagt ggagtatgct actgtcgaca ccgagcaggt tgcccagtgg ccgtgggatt
     4021 ccattctcag cagcttcagg gaactcatcg gcacttacga tcagggagtc agggaccttg
     4081 cagccaagct gcagcagtcc cacaaaaact cttccacaag agatgcttta gaagatatcg
     4141 tccagcagtt taccgacgaa gtgaaaaagg ccatggccca gcgagaagag gagatcggga
     4201 aggctttgtt ccaagagatc tcccgcgtag ttacgcttag aaacgtcgat caagcctgga
     4261 tggatcattt ggataacatg gatgacctca aagaaggcat cggcctaaga gcctatggcc
     4321 agatgaatcc tctcatcgag tatcagaagg agtcttcacg gctctttgaa gcaacgctta
     4381 tgagaatcga agaggagact attaggcttc tcttccacct caggctagcc aacgaagaag
     4441 aagtgaagag agcagaagat agggaaaaag ccaagctcag cgcacttcag agcaacagga
     4501 acgctgcgga agcacaggcg gcaccggtgc aagccaaaaa agaaccagga cgcaacgatc
     4561 cgtgcccctg cggcagcgga aagaaataca agaaatgctg tggtaaataa ggagtgaacg
     4621 tggtttgaat ttggaaatga ttcctgtcgt taaagacaga attaaacagg tagagaaaca
     4681 ggttaaagca atgggtgagt atctttgaaa ggtctaaagt agacgaaagg atcagcgctt
     4741 tagagcagga ggttttggct cctaacttct ggtccgacca aagacttgcc caaaagaaaa
     4801 ctaaagagct caacgaactg aaagagagca gggatactct gcagaagctc gctcgcgatt
     4861 tggaggactt aaaagtcctc ttggaccttg cggtgagcga ggaagatgga gataccctta
     4921 acgatgtgat tgcagaaagc gaaaggctgt tgaagtttgc cgagcgctac gagttggaga
     4981 tgctccttaa cggtccttac gattcttctg acgctctttt gactatccac cctggagcag
     5041 gcggaaccga atcacaggac tggggaagca tgttgtatcg catgtacatg cgctgggctg
     5101 aaatcaacga ctatacagct aaggtgttgg atttccagcc cggagacgaa gcgggaatca
     5161 aatcggtaac tatctccatt tccggaccct acgcctacgg ctacctgaag gcagaggccg
     5221 gggtgcaccg cttggtcagg atatctcctt ttgactctgc aggcagaagg cacacctctt
     5281 tctgctctgt agacgtgatt ccccagatct ccgatgctgg cgagattgag atcgatccca
     5341 aagatctcga gattgacacc taccgctcag gaggtgcagg tggacaacac gtaaacaaaa
     5401 ccgactcagc agtacggatc acccacattc ccacagggat agtggtccag tgtcaaaacg
     5461 aaagatccca gcacgctaac aaggaaacgg ccatgaacat gctgatgtcg aagcttgtag
     5521 ctctcaagtt gcagaagcag aaggaggagc tggaagctat aagaggcgag aaagccgaaa
     5581 acacgtgggg aagccagatt cgttcgtacg ttttctgccc ttatactatg gtcaaggacc
     5641 accggacggg acttgaaacc ggcaacgttg acgcagtaat ggacggtgcc attcaagact
     5701 ttatcgaggc ttacttgaga atgaagtagc agagggatca gaatgaaaaa acaaaggaag
     5761 ctgggtagat ggtttctgcc cagccttaca ctggttttac tcctgcttac catcgcgttt
     5821 tatttaccgg ttctagtcac agatcagctg accaaagcta ttgcagtgaa tctgtacagt
     5881 tttgacagct tgtccgtcaa acataaagcg agccctgctt ttctgttgtt ttttgggaaa
     5941 atcagggatc taaccatcga gggttctaac cttgaggggc agtacatcaa cctgtccaga
     6001 gttttcctgc aggtagacaa gctgaaagtg tccatgtggg acctactcgt ttacggcagg
     6061 ctgaacattt tggacagcga aggaagggcg gccctttcca tcagtcaagc agagctggac
     6121 aagtacctcc accgctttta tatcgatgat caatatcata gtatacgatt agatcttcta
     6181 gatgatggta taagaataag tggaacccta gatggcagag aattctatct cgattgcgtt
     6241 tttaccgttg aacaaaacag cgttttggca ctgagcccca agagaatggt ttcaacaaat
     6301 ggcagcaacc agaatctcat gaacagactt ggccaaatgg ccagctactc aatagatctg
     6361 ggagtttttg gatttcctct aattattgac gatgcgtctt ctagagatgg ctatctgtat
     6421 gtctttgcaa gtgtcagcaa ataaaggaag gaattaaaat actgccgtgg aaaactttta
     6481 taccaagggg gtgaccgaaa ggtgaaggtt ctggaaaaaa acacgcagaa tgaacttcag
     6541 gtagtcgtgt tcaaaatcaa agatgaaatg tttgctgcag atattcacca ggtaaaggag
     6601 attagaggcg tcgacaaagt caccaaggtg ccagatgctc ccgacgttct cgaaggcatt
     6661 attcatctta gaggtcagat ttacccgatc gtcaatctaa gtaaaagatt ccggaaagac
     6721 aaatatgaga ttactcccca gagtaggact atcatcatcg atctaaaagg ttcccgcttt
     6781 ggagtgattg tagacgaagt aactgaagtg atcagggtta agaaagaaga catacagagc
     6841 actccagata ttctcagcag ccaaaacggc tatttggaag gcgtaataaa aagagatgaa
     6901 cagttgattc tgtttgtaaa tctagagaaa atccttacgg ataaagaact aaacgaaata
     6961 aacaaaacga aagatctcta agctggagga agaaaatggc tattaagcta gatgttaatc
     7021 aaagggtgca ggtgagcaga accacatctg gagacgccaa aagagaatac tatcccagca
     7081 ggatcgaaga aattaccaat gatgcgattt ttattgcaga accactagat ggtactgttc
     7141 ctgtgtattt gcgcttggga gaagagatcg aggtgttctt caacaccgaa gcaggcactt
     7201 tccgattcac atccaaagtc ctgcagcgct tagagcgcaa catccgcatg gttaagatct
     7261 ccaagccaga gacttttacc aagagcaatc gcagggattt cttcagattg aaagtgttta
     7321 ttccgtttta ctttagagtg ttgcctgaag ccacgttcaa ttacggtcat ccgcataaac
     7381 aggaagacct gaaaaagaca attcttgaca agcagctggc tctagacgaa accggaaagc
     7441 aggaaggaat tgtcaaggat ctgagcggag gtggacttct cgctgccgtc tcggagaaat
     7501 ccggtcttaa gttaggagac catttggaaa tgtggttgcc tctagacaat gaaggcgtct
     7561 acgtttgggg ggacgtggtg cgaattttgc acaatcccga tgccactaag tacaacgtag
     7621 atgtcggcat tcacttcagc aagatcgagg aaaaagtccg agatcagatt atttcacacg
     7681 tgctttctct gcagagagag cttctgcaaa aaggtgcact agagtaagaa aacacgaagg
     7741 actgccgctt ggcagtcctc ttatctaacg gggggcgctg tatgaaaaag ggatggattt
     7801 ggattgtcgt tataggactt ctctgttcgg cttttgccat ctatcaaagg gtacgtatcg
     7861 aacaagcgga ccgaaccgtg cagctggtct tggatagtag gtccttagca gagtatgctg
     7921 cagacaaggg gatacccctg gaggcgctat atgcagaggt cgcagatctt gacgctttcg
     7981 acctggcaat ttctgaagtc aaactttggg aatcggtagc ctctagatcg acggtggtct
     8041 accccggttt ctacctactg ttttccagcc cggaaatgga agggacagac ccgcggaagc
     8101 tttacatcgc tgacgacact tctggaccga tcgcaggaag gctgagtccc ctaggggttc
     8161 cgttttttgc cgacgatgcg agactttggg agttccaaga ggagtaccct gttgatgtaa
     8221 accgaaggaa agaagccctt agagagccta ccggttcttt gtgggacgaa gcactttatc
     8281 ctgactactc atcccttgat cttgcccagt gctttggaat gcaggttgtg gcgcgaatcc
     8341 aaaatccata cgtgcataca ccagaaagtt taaaatatgc agtagaccag tgggatggcg
     8401 cggggaacct cgttatcttt gaagggaaag aagtcctcgg ctatccaggc aatctgaaaa
     8461 gtgcagcaga actcctcggc gatacgcctt ggggatttat cgagtttgcc aatcaatacg
     8521 gcgaaaagga tcttgccaga ctaaccaact acaatctgat cagggttcac agcatcaccc
     8581 ctggagagat gctgaagatc gatccgcaag tggccatgga gagatacctc agggcagtgc
     8641 gagaacgcgg agctcgagtt ctctacttga gaccctttcc caagctaggt cttgaggaaa
     8701 accttgcttg gtttgcaggt cttgagcaga acctgaaaga tgacggctac agattaggac
     8761 aggcagagcc caagccgttt tttaagagtt cgttcttgat tttcttacca gtgcttcttg
     8821 ctgtagtcat ctgcggtatt aggttaatgc tgcttctaga gtttagggaa accacttctt
     8881 tgttttctgg tctctttgct ctcgtcgctg tcgcttctgt ttatctaaaa gggtacacta
     8941 tactggcaag acaaatcacg gctttcggcg cagcggtgat cttccctaca cttgccgttg
     9001 gttttgtggc caagcagata ggggaaggaa aagcaagact ctggcagacc atgggcacgg
     9061 tactctttta caccgctgct gggagtatgc tgctttcagc agccctttgc gacctgcgct
     9121 ttgtgattaa aatagatcag tttttaggtg taaagctgat gcacatcatt ccgccccttt
     9181 tgaccttgtg gatctccatc aggagcatca gtaggggggc tttcggggca gcaggcttta
     9241 agagcaacat tagggcaact ctcagttctc tctggccgcc gaggctgggg cacatcattg
     9301 ttctggtgct tgttctcttt gctggttttg tctatattgg caggacaggc cacgactggg
     9361 ggctgccggt cttggagcta gaaacaaaaa tccgagtgtt tttggaggac ctttttgtga
     9421 tcagaccgag acttaaagag attttcatcg gtcatcccgc tttgttctta ggattgttta
     9481 ttgcttcttc ggtgctgatc cgaagactga agtggctttt gcctttgatt ttgactgtag
     9541 gctccatagg tgtaacgtcg gtcctcaaca ccttcagtca cgctcacaca ccatttctcg
     9601 tatctgtagt gcgcaccgga tggggttttg ccatcggaat gcttctcgga cttgtggctt
     9661 ttgggctact tagactgata gtaagggtag agaaccatgg gtaaagttct agtctttggc
     9721 tactacggtt ttggcaactt tggcgatgaa ctcctagttg cagcgatcaa agagcatttg
     9781 caggaccacg atcttacggt ggtcagcgga aatcccaaag agaccgaaaa aatccacgag
     9841 ctgaaggcca tctccaggaa agacttctgg aagaacccag gttcctacga catcctgctc
     9901 ttagggggag gcggcctttt gcagagtgta accagcaacc gctccctgct gtactacctt
     9961 gcagcaattc gctgggcaag agcaaaggga cttagcgtct acctgatggc ccaaggtcta
    10021 ggtccgttca agaaggggcc catttatccc ctggttgacc tggggatgag gtccttaaaa
    10081 ggtctcgaga tttccgtgag ggacaaaaaa tcaaaagacc ttgccgcaag ctttgggctt
    10141 gaagcagatc tcgtggcaga tctcgctttg acttacactg gattccccac aagaaagacg
    10201 ggagaaggga ccaagagagt catcggcatc gctcccaaag caggttttga tgtgaggcag
    10261 attaggtggc tggagtcttt cggagacgaa acaaccgtgc gcctctttgc ttttcataaa
    10321 gacgatcata gggtatgctt agaagtggag aaactcctga aggatgctca tactataaaa
    10381 atggaaaaaa cccaggatct tgagaatttc gctgatatcg actttctttg ggggatgagg
    10441 ctgcactcgc taattgttgc agcaaagatg ggcatccccg ccattggcat tagctacgat
    10501 cctaaagttg agcaattctg cagagaagtg ggctttgctt ggcacagccc ggagaccatg
    10561 ccgaaggagg agtggatcag gagtggcaat gcaacagaac tcatccaacg agcagaaaaa
    10621 gcctgggccc tgttcagaag ctacgccgag acccaagcaa atcgaactcc tggacattcc
    10681 cattgacgct gtggactttt ccggcgcatt agcaataatt ggcaggttta ttgccgagga
    10741 gagaaaaaat cccgcgctgg tctttactcc caacgcggag attttagtta aagctcatga
    10801 cgatgcggag ttcggcaggg ttttgaaaaa tgccgatcta gtcgttcctg atggaaccag
    10861 cctcttatgg gcatcgaagg tgttaaaaag gcctctggag accagggtga ccggcatcga
    10921 tctcatgcag cgccttctca cagaggcaga aaatgccggt tggtcggtgt acttcctcgg
    10981 agcgaaacct gatgtgatag tagaagcggc agcaagagtg aagcatctcc accccggtct
    11041 taaactggta ggctatcacc acggttattt cgatgatgat ttctccgtca tagagggtat
    11101 aaataaattg cgaccagata ttttgtttgt tgggatgggc gctcccaagc aggaactttg
    11161 gctgaccaga aatcgcgaag aactcaactg ccgcgtagcc cttggtgtag gcggaagctt
    11221 tgatgttctg tcaggaaggg tggatagagc cccgctctgg atgcagaaaa aaggtctgga
    11281 atggctgtac cgctacatca gagaaccttc aaggctcaaa aggagcatac tccttcctcg
    11341 atttgtccgt ttggtcctca aagaaaagtt cgggacctca aagggggaga caacgcagtg
    11401 acttctcgcc tttttaaaga gatcatgcgc atgcttgggg tgcttgcagg gactacggtc
    11461 ctggcacttt cgcttgtgct ttttctaatc cccaacaaaa tcgctccagg gggagttagc
    11521 ggactggcaa cagtagtcta ccacttgtca ggtctcagtg ttggactggt catgctgctc
    11581 atcaacattc ccctcttttt acttggcata aaaaaactgg gatttgtctt cggtctgcga
    11641 tcgttggtag ccactattta tctctctttt gccgttgata ttttgcagag gtacgtgagc
    11701 ccagttaccc aagatcccct ccttgcagct ctgtatggtg gtataataat gggagtcgga
    11761 ttgggtattg ccataaccaa cggtggaaca accggaggta cagaccttgc ggcagtactt
    11821 gtccacaggt ttctgggact ttctgtaggt gccacacttc ttgtgataga tgccattgtt
    11881 attgtaattg caggattttt cttcagttta gagctggcca tgtacagcgc tttagcgctt
    11941 tttgttgcta ctaagctgct cgatctcatg caggaaggtt ggcattacgc cagagcagct
    12001 tacattatct ccgacaagtc cgatgatata gctcaggcta tcatgaccaa aatggaaaga
    12061 ggcgtaaccg gattttacgg caaaggcatg tactccggca aagataaaaa catactgtat
    12121 gtaatctgtt ctcgttccga aatagcgacc ttaaaagact tggttcggac cattgataaa
    12181 gacgcattta tcgttattgg agaagctacc gaggttttag gcgaaggttt cagagccaat
    12241 aaaggtacaa atgagtgaaa tcgaggtgta aactatggac gataaaaaac ttgaagaact
    12301 caaggagata gccaaagagt ccagggtttg gattatcaaa tcccttgcag aagcgaagtc
    12361 aggtcatcca ggaggtagcc tctcctgtat tgatattctt acctatcttt acttccatgc
    12421 tatgaatgta gacccgacaa accctgacga tcccgatcgc gacaggtttg tgctatcgaa
    12481 agggcactgc gcgccagctc tttacacggt tttggcactt aaaggcttct tccccaaaga
    12541 agagcttttg aagctgcgga agctcggttc catactccag ggacacccag atgccgttaa
    12601 ggtgcccgga gtagatgctt ccacaggttc tttaggacaa ggtctctcca ttgcaaacgg
    12661 aatggcttta gctgggaagc tagatcgcaa agattatacg gtctacacca ttatcggtga
    12721 tggagaaact gaagaaggtc agatttggga agctgccatg accgcagctc actaccaact
    12781 tggcaacctc atagcttttc tggactataa ccacttgcag atcgacgggc gcatcgaaga
    12841 cgtcatgggc aacggcaact tcaccaaccg cttcaaagcc ttcggct
//
LOCUS       DAOSDA010000003        12645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_3349_length_12645_cov_3.405322, whole genome shotgun sequence.
ACCESSION   DAOSDA010000003 DAOSDA010000000
VERSION     DAOSDA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12645
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3349_length_12645_cov_3.405322"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..637
                     /locus_tag="PLW82_00135"
     CDS             11..637
                     /locus_tag="PLW82_00135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOJ45718.1"
                     /translation="MAQIRGRLLDGPTEAEFAAELDTAMRSEGAADVSFETIVASGPN
                     GAKPHHTPSDRRIAPGDLVVVDFVALVDGYHSDMTRTVAVGEVSAELKKIYDIVYKAQ
                     MASLEAVAVGKTGAEIDKVARDIIQEHGYGDYFGHSLGHGVGLDIHEGPRLSAASDTV
                     LEENMIITIEPGIYLPDVGGVRIEDLVVVEKTGARRLSLSSKDLIVLS"
     gene            651..1208
                     /gene="efp"
                     /locus_tag="PLW82_00140"
     CDS             651..1208
                     /gene="efp"
                     /locus_tag="PLW82_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOJ45719.1"
                     /translation="MISSNDIRPGVTLLLEGSVWVVVEFLHVKPGKGAAFVRTKLKNV
                     ETGQTLERTFRAGEKLERAQVSSREMQYLYEMEGNYYFMDTETYEQTFIPEDVIDNGK
                     WYLKENDVIIIQFYQDRPISVELPTYADLVVVETDPGLKGDTASGGSKPAKLETGVVV
                     QVPLFVQVGDKIRVDTRTNEYLSRV"
     gene            1252..1662
                     /locus_tag="PLW82_00145"
     CDS             1252..1662
                     /locus_tag="PLW82_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45720.1"
                     /translation="MSEVREKVAYLKGLLAGLGKLEDPASAKIWEAVLDVFDTVADEL
                     EILDDDLEDLGQYVSSIDDDLAEVEDNLYEDYVDDESDDTIAIECDTCGHEFSIPEYK
                     LYNDEDVVCPECNTSIYDDLDDDDDVEIGFSDEE"
     gene            complement(1730..2413)
                     /locus_tag="PLW82_00150"
     CDS             complement(1730..2413)
                     /locus_tag="PLW82_00150"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HOJ45721.1"
                     /translation="MPKRALLLLIVLCLSSLALYASGPLTILKSTLSFEREKITIYVE
                     YPLVSGTENPEVASKINSTIESDALGFFDYFRGIALAGVDEVMTAYAGHVSYEVVLNT
                     SNLLSINIIYYEFTGGAHGMTEMINYTFNTTTGERLKLEDLFKEGTNYVQAIIEEVVK
                     QIEKTPEMFFDDAVDVVRESPFNFYLAPDAFVAYYGLYAICPYAHGIPEFKIPYELLR
                     DYLLVPLGQ"
     gene            2494..2763
                     /locus_tag="PLW82_00155"
     CDS             2494..2763
                     /locus_tag="PLW82_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOJ45722.1"
                     /translation="MAENTKSPKKDIVNRLSRIEGQVRGIKGMIEEDKPCLDVLAQIW
                     AARAALHKAAELLMENYVKECLVKDQENFESSVEELVKAIKAIMS"
     gene            complement(2855..3832)
                     /locus_tag="PLW82_00160"
     CDS             complement(2855..3832)
                     /locus_tag="PLW82_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HOJ45723.1"
                     /translation="MINGTFFEFLHHNEKETTWNHQAAQFTDEMWAEKVDEMAEIGIE
                     DLVITFVALNYKTFYPSRIFPKWDIKAQDPLYAVLHAAERNKMRIFVGAGFYGNWTNP
                     WEAFTDMEVRRRTEEAIQELAENYGDFRSFYGFYWPNELGIGPDGHFPQVFIDHVREC
                     NGFAKRYLPKAKTLIAPYGTNMAIPDDLFVSQLKEMGIDFVAYQDEIGARKTKIEQLP
                     QIFSGLKEAHRKADIPLWVDMEIYEFEGEILVSKLLPGDFARIEKQIEALSPIVDRIL
                     VYQYQGIMNKPSTKVFTGVKESEKLYSDYVAKFNPASRQKFSKFPNRPV"
     gene            3967..4803
                     /locus_tag="PLW82_00165"
     CDS             3967..4803
                     /locus_tag="PLW82_00165"
                     /inference="COORDINATES: protein motif:HMM:NF040318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HOJ45724.1"
                     /translation="MLNYLAVNLKTHIGSLINSATEIRVRLGRPLTLRFFAQEVGVYM
                     GKVCPLEAAYIASERDIAETLDRITEASLYAFREQFKQGFLMLPGGHRVGITGEVSLA
                     ESGVYMQRHISSLNIRLAKDVPGAAAFLYPHLSCGPKHTVVVGPPGSGKTTVLRDLVR
                     FLSSSGLTVGLIDERGEIAASYKGLPGFAIGHTVDVLTGCPKREGFEILLRGMRPDVI
                     ATDEIGFGDDLESMKTASQMGVKVVATWHGTEPPKSFFEIGALLGPVPGTEPLFVGRL
                     DS"
     gene            4800..5282
                     /locus_tag="PLW82_00170"
     CDS             4800..5282
                     /locus_tag="PLW82_00170"
                     /inference="COORDINATES: protein motif:HMM:NF021090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HOJ45725.1"
                     /translation="MKILGQLVILFTGLILGMWGVRREEKYISLIEELQLGLSIVENE
                     ISFALRPLPEAFTHAAKSLKAAKVFFLDAARRAKDGETCLFNVDLLPLRDDEANILFE
                     VGIRLGRGLDREQIALILKAVSRLEESRQTCRERLKRVSPLWRWLSPLAALLIVLLSC
                     "
     gene            5295..5489
                     /locus_tag="PLW82_00175"
     CDS             5295..5489
                     /locus_tag="PLW82_00175"
                     /inference="COORDINATES: protein motif:HMM:NF018401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HOJ45726.1"
                     /translation="MQIGVIMQLVGLGIIAAVVHIVLKQAGKEDIALIVTIGAVVIGF
                     ILILDEISRLFSSIRLLFGL"
     gene            5503..5892
                     /locus_tag="PLW82_00180"
     CDS             5503..5892
                     /locus_tag="PLW82_00180"
                     /inference="COORDINATES: protein motif:HMM:NF018401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HOJ45727.1"
                     /translation="MAIWQVVGVGIITLVASLVLKKYLEEASLPVLLAGLLVILGLIF
                     EPLKLLWETFREFGHISGINFAYLELIGKILGTAFLAGLAADICRDCGESALAAKIEL
                     AGRIIIIVLTLPVLQAVLTGLLEVLPR"
     gene            5889..6962
                     /locus_tag="PLW82_00185"
     CDS             5889..6962
                     /locus_tag="PLW82_00185"
                     /inference="COORDINATES: protein motif:HMM:NF021088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HOJ45728.1"
                     /translation="MKWIALLLVVLSAMSSAAALDTLEIPTELERLLPDTIEPYSIEE
                     FLRDPSAEKVLSTLWQYLWKEVRLHARLMGQIILVAAIAAVLKEIGTSLSKAAAEAGF
                     LATYLMLVLLVVASLKEAANLTTSTIVLLASAVQVAVPLVGLFLAAGGELVTNATLSP
                     LILGLTAVVSVVVQRVVMPFALASGAIGLASSVAEKPRLLRLSGFFRWLGTLTLGFVN
                     AVFFGLITSLGLGSAAQDSLALRTAKWLMGTVPVVGGQIAGTTDLLQASTRAIQSAAS
                     TVGLLFVFFATLFPVLRLAALVLAYKLTTAVVEAVAETRFVSALETMEKTLTFFLGLI
                     VLTAVMFYIGLGTLAALGALILR"
     gene            6968..7180
                     /locus_tag="PLW82_00190"
     CDS             6968..7180
                     /locus_tag="PLW82_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45729.1"
                     /translation="MNEIWQTLAKLIGLCFLEEMSCLFIHDEKLGKTVRLVFGLLFLA
                     VLLGPLLNLFQIGDLLSQIDFEGLTK"
     gene            7180..7683
                     /locus_tag="PLW82_00195"
     CDS             7180..7683
                     /locus_tag="PLW82_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45730.1"
                     /translation="MLEKIKEVLQKIPWPLFVVGFVGVVLLIWGSLPEKKPAPVIEAT
                     LERPQIGEEEKLAQLLSSLGIGSVKVMITYEDEGKTHVAEDKSVETTYDGSGVRSVKE
                     STEVVRMKTASGETGLVLVQESPKVSGVVVTTSKPLSTAQKLRIIEAIRALWDLPLGR
                     IVVLGKE"
     gene            7691..8272
                     /locus_tag="PLW82_00200"
     CDS             7691..8272
                     /locus_tag="PLW82_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HOJ45731.1"
                     /translation="MKRAIGWFVLLLVLSTGITVWWNFGGREAVSSLRFSLPVFGGRV
                     DDIDYKKYENFDIPEDDPLLLDPKEISYVREEEDPEVVAVVASYDPLIEYRLARDIAR
                     ENARIGLERLANSGSVDASIELLNQTRNVSKEEELESILKAKGFGDTVITIEDNMVKV
                     IAKDLNKQKVEAIAEIIYNLTTFGKDQIVLTSI"
     gene            8436..8891
                     /gene="accB"
                     /locus_tag="PLW82_00205"
     CDS             8436..8891
                     /gene="accB"
                     /locus_tag="PLW82_00205"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOJ45732.1"
                     /translation="MDLRDVRDLIKLLDESNLTKLEIEDPNGFKLKLEKNCSEAASKS
                     VPVRGAESVLEDEQKLDNTISARDLDQELKTITSPMVGTFYRAPAPDAEPFVKEGSTV
                     DVGDTLCIIEAMKLMNEITSEYRGRIVKILVENGQPIEYNQPLFLLERV"
     gene            8907..10256
                     /gene="accC"
                     /locus_tag="PLW82_00210"
     CDS             8907..10256
                     /gene="accC"
                     /locus_tag="PLW82_00210"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOJ45733.1"
                     /translation="MFRKILVANRGEIALRIIRSAKEMGIKTVAIYSEADKESLHVQF
                     ADEAYCVGPAEQSKSYLNIPNIISSAIVSGADAIHPGYGFFAENAEFAEVCSDNGFRF
                     IGPSKEAIALLGDKAQAKNLMQEAGVPVIPGSPGCICSLEEAMAFAREVGYPVIVKAA
                     QGGGGRGMRVARNEGELKKAYDLARTEAEKAFESGEVYLEKYVENPRHIEVQILIDEL
                     GNGIYLGERECSLQRRHQKVLEESPSVAVSPELRQKMGEAAVRGALAAGYTNAGTMEF
                     LLDSQGNFYFMEMNTRIQVEHPVTEMVTGVDLIKEQIKIAAGQPLSLKQEDVVIKGHA
                     IECRINAEDPDKGFRPAPGPIQGFLLPGGAGIRVDTAAYAGYEIPPYYDSLFAKLIAW
                     GNDREEAICRMRRALDEFIVEGIPTTIPFHKKVLENEHFLQGDIDTAFIAKHILKDH"
     gene            10318..10722
                     /locus_tag="PLW82_00215"
     CDS             10318..10722
                     /locus_tag="PLW82_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOJ45734.1"
                     /translation="MGEQILVNTERSDLGQVRIADEVVGIIAGLAASEVEGVHSMSGG
                     ITGGIYDMFGRKNFSKGVKVEVGQVEVAVDIAIFLEYGYRVPDVATRVQENVKKAIES
                     MTGLTVVEINVHVQGIHFPQTQVPEEKETRVK"
     gene            10827..11363
                     /gene="amaP"
                     /locus_tag="PLW82_00220"
     CDS             10827..11363
                     /gene="amaP"
                     /locus_tag="PLW82_00220"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HOJ45735.1"
                     /translation="MNWLDKVLIFVASLVLAILGITLILLGLGVDFWNSFAGAHDYLA
                     TNALVSFLVGAVLCIIGFYLAGNLLGEKEEEVGIVAENEIGKVNIAERVIKAYAERAA
                     QSVEGVTGVKSKIKSQAEGLSIVLDLSTNTSVKTADTAKHVQERVMEYVSDSLGTKVT
                     SVEVIISSVEESKPHRVS"
     gene            11390..11617
                     /locus_tag="PLW82_00225"
     CDS             11390..11617
                     /locus_tag="PLW82_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014459855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HOJ45736.1"
                     /translation="MSIIKEIFDRLSPYKGRIIGVLFGLILAILVLSIGFLRALVVGL
                     FVALGFYLGKKADNQESLVDLWERLRRNRNG"
     gene            11610..12005
                     /gene="nusB"
                     /locus_tag="PLW82_00230"
     CDS             11610..12005
                     /gene="nusB"
                     /locus_tag="PLW82_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017727016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOJ45737.1"
                     /translation="MDESTKRRTKRERAVELLYQLELTGKSLDEVFSYADPPVLEDER
                     ALIVGVWEKRHEIDEMISRTSREWKLDRIGYVERNILRLALYEINYDDVAVPYAINAA
                     VELAKEYSGEEAGRFVNGVLGRAVREKSE"
     gene            12190..>12645
                     /locus_tag="PLW82_00235"
     CDS             12190..>12645
                     /locus_tag="PLW82_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HOJ45738.1"
                     /translation="MFEEMGPLADVQFGVTTRPRPVEGSYMPCFVVGESFVRALAAGR
                     GYLAISHQENHLWAGLYENRTLIGKPFIAVHLSGGTTEFLHVTWNDQNLQIKEVGQTT
                     DISAGQFLDRTGVQLGFPFPAGPYLEKIARQSDLRVGVTVKGTAISYSGP"
BASE COUNT         3166 a         2921 c         3470 g         3088 t
ORIGIN      
        1 ggaccgagcc ctggcccaga tccgagggcg cctcctcgac gggccgaccg aggccgagtt
       61 cgccgccgag ctcgacaccg ccatgcgctc cgagggggca gcggacgtga gcttcgagac
      121 gatcgtggcc tcgggcccga acggggccaa gccacaccac acgccgtcgg atcgtcgcat
      181 cgcgcccgga gacctcgtgg tggtcgactt cgttgccctg gtcgacggct accactccga
      241 catgaccaga acagtggcag taggtgaagt ctccgctgag ttgaagaaga tctacgacat
      301 cgtctacaag gcgcagatgg cctctcttga ggctgttgct gttggcaaaa caggagcgga
      361 gatcgacaaa gtagcaagag acatcatcca agagcacggg tacggagact acttcggtca
      421 cagcttaggc cacggcgtgg gtctggacat tcacgaaggt ccgaggctat ctgccgcatc
      481 agacacggtt ctagaagaga acatgattat taccatcgaa ccgggcatct accttccgga
      541 cgttggcggg gtaagaatag aggatttggt agtggtggaa aaaacgggag ccagaaggct
      601 ttcactgagt tcgaaagatt taatagtctt aagctaagga tgtggtatgt gtgatttcta
      661 gtaacgacat tagaccggga gtaactctac ttctcgaagg ttcggtttgg gttgtagtgg
      721 agtttctgca cgttaaaccc ggaaaaggtg cagcttttgt caggaccaag ttaaagaacg
      781 tagaaacggg ccagacctta gagcgcacat tccgtgccgg cgagaaactc gagagagccc
      841 aggtaagttc cagagaaatg cagtatctct acgagatgga aggcaactat tacttcatgg
      901 ataccgagac ttacgaacag acatttattc cagaggacgt tatcgataac ggcaaatggt
      961 atctcaaaga aaacgatgtc atcattatcc agttctacca ggatcggccg atctccgttg
     1021 aactacctac ttatgccgat ctggtagtag tggagacaga cccgggtctg aagggagaca
     1081 cagcatcagg aggttccaaa cctgccaagc ttgaaaccgg agttgttgta caggtacccc
     1141 tgtttgttca agtgggagac aagatacgcg ttgatactcg taccaacgaa tacctcagtc
     1201 gcgtctaggc tatcatataa ttatcagatt taggcaaagg agggctttgg agtgagtgaa
     1261 gtcagagaaa aggtggcgta cctcaagggt ctactggctg gtttaggtaa gctggaagat
     1321 ccggccagcg ccaagatttg ggaagctgtg ctggacgtct ttgacacagt cgccgacgag
     1381 ctcgagatct tagatgatga tctcgaggat ttgggccagt acgtaagttc catcgacgat
     1441 gatcttgcag aggttgaaga taacttatac gaagactacg ttgacgatga gagcgatgac
     1501 accattgcta tcgagtgcga tacctgtggc catgaattct ccattcccga atataagctt
     1561 tacaatgacg aggacgttgt ctgcccagaa tgcaacactt ccatttacga tgacctggac
     1621 gatgatgacg acgtagaaat tggtttttct gatgaagagt agatttgagt aaaagaaaaa
     1681 actcacagcg cgtctgtgag tttttttgcc ttctgaggca tatggggttt tactggccaa
     1741 gaggtaccag taggtagtcc cgaagtagct cgtaggggat tttgaactct ggtatcccat
     1801 gggcatatgg acagatggca tagagaccgt agtaggctac gaaggcatct ggagctaagt
     1861 agaagttgaa cgggctctct cgaactacat caacagcatc atcgaaaaac atctccggag
     1921 tcttttcgat ctgcttgaca acctcttcta taattgcctg tacgtagttt gtaccttctt
     1981 tgaagaggtc ctcgagcttt agcctttctc ctgttgtggt gttgaaggtg tagttgatca
     2041 tttcggtcat cccgtgggca ccgccggtga actcgtagta gatgatgttg atgctgagaa
     2101 ggtttgatgt gttcagcaca acttcgtagc tcacgtgtcc tgcatatgcc gtcatcactt
     2161 cgtctacacc tgctagcgca atacctcgga agtaatcaaa gaatcccaag gcgtcgcttt
     2221 cgatagtgct gttgatttta ctggcgacct ctgggttttc ggttcccgaa accaaagggt
     2281 actccacgta gatggtgatc ttttcccgtt caaaagacag agtgcttttg aggatggtta
     2341 ggggaccaga agcatacaga gcaagggaac tgagacagag aacaatgagg aggagcagcg
     2401 ctctttttgg catgtaatca ccccgaagtc agattttgcc tgatcaggca atggcagaac
     2461 tgaactggaa agcattggta aggaggcagg actttggcgg aaaacactaa gtcaccgaaa
     2521 aaagacattg tcaacaggct gagccgcatc gaaggtcagg tgcggggcat caaaggtatg
     2581 attgaggagg acaaaccctg cctggatgtg ctcgctcaga tctgggcggc acgtgccgct
     2641 ctgcacaagg ctgccgaact cctcatggaa aactacgtta aggaatgctt ggtaaaggac
     2701 caggagaact tcgagtccag cgttgaagaa ctggttaagg ctatcaaagc catcatgtcc
     2761 tgacttctgc cccgcagagc agtagatggc taaccggaaa actccataga gtgcgaacga
     2821 aaagaagcgg agggaaagcc ctgcgcttct ttttttacac aggcctgttg gggaacttcg
     2881 agaacttctg cctggaagct ggattgaatt ttgccacata atccgagtag agtttctccg
     2941 actccttcac gcctgtaaaa acttttgtgc tgggcttgtt catgatgccc tggtactggt
     3001 agaccaaaat ccgatcgacg atcggcgaaa gcgcttcaat ctgcttctca attctggcaa
     3061 agtctcccgg aaggagcttc gacacaagta tctcgccttc aaactcgtag atctccatat
     3121 ccacccagag ggggatatcc gcctttctgt gggcttcctt cagcccgctg aagatctggg
     3181 ggagctgttc tattttggtt ttccttgcgc cgatctcatc ctgataggct acaaagtcta
     3241 tgcccatctc cttcagctgg ctcacaaaca ggtcatctgg gatggccatg ttggtgccgt
     3301 atggagcgat aagcgttttg gctttaggaa ggtagcgttt ggcaaagccg ttgcactccc
     3361 gtacgtgatc gatgaagacc tgggggaagt gcccgtccgg tccaataccc agttcattgg
     3421 gccagtaaaa gccgtagaaa ctgcggaagt cgccgtagtt ttcagccagc tcctggatgg
     3481 cttcttccgt tctcctccgg acttccatat cggtaaaagc ttcccaggga ttggtccagt
     3541 tgccgtaaaa acccgcgccc acgaagattc gcattttgtt ccgctctgca gcgtgcagga
     3601 cagcatagag gggatcttgg gctttaatat cccacttggg aaagatcctt gagggataaa
     3661 aagtcttgta atttagtgcc acaaaagtaa tcaccaggtc ttcgatgccg atttcggcca
     3721 tttcatcgac cttctcggcc cacatttcgt cggtgaactg agccgcttgg tggttccatg
     3781 tggtctcttt ttcgttgtgg tgcaaaaact caaaaaacgt accgttaatc atttcgaggc
     3841 ctccctattc tatcagtttg gtatttgggc aaagcccggg caattcctgc accgatgtac
     3901 agcctgtcat aaaagagtcc gaaggttaat atgatgtagg gagattgtcc ggatgggaga
     3961 tttgccatgc tgaactacct tgcggtaaac ctcaaaaccc acattggcag cttgattaat
     4021 tcggccacag agatcagggt ccgtctaggt cgtcccctta cacttaggtt ttttgcccaa
     4081 gaggtaggcg tgtacatggg gaaggtctgc ccgctggagg cagcttacat cgccagtgaa
     4141 agggatatcg cagagactct ggaccgcatc acagaagcat ctctgtatgc ttttcgcgag
     4201 cagttcaaac agggcttctt gatgctgccg ggtggccacc gggtgggtat cacaggcgaa
     4261 gtcagcctgg cagagtctgg ggtgtacatg cagcggcaca tcagctctct taacatccgc
     4321 ttggccaagg atgttcccgg tgcagcggcc tttctgtacc cgcacttaag ctgcggcccc
     4381 aaacacacag tggtcgtagg tcccccggga agcggcaaga ccacggtact cagagatctg
     4441 gtcaggttcc tttcgtccag cggccttacg gtgggactta tcgatgaaag gggagagatc
     4501 gccgcttcgt ataaagggct gcccggtttt gccatcggac acaccgtaga tgtccttaca
     4561 ggctgtccca aacgggaagg ctttgagatt ctccttagag gcatgagacc ggatgttatt
     4621 gctacagatg aaatcggctt tggcgatgac ttagaaagca tgaaaactgc aagccaaatg
     4681 ggggttaaag ttgtggccac ttggcacggc acagaaccac cgaagagctt ttttgagata
     4741 ggcgcgctcc tcggcccagt tccggggaca gagccgctgt ttgttgggag gttggattca
     4801 tgaagatctt aggacagctg gtaatccttt ttaccgggct gattttgggt atgtggggcg
     4861 tgcgcagaga ggaaaaatac atcagcctaa tcgaggaact gcagctgggc ctgtccatcg
     4921 tggaaaacga gatctccttt gccctgaggc cccttccgga agccttcacc catgctgcca
     4981 agtcgttgaa agccgcaaaa gtcttctttc tcgatgctgc cagaagggcc aaagatggag
     5041 agacctgttt gtttaatgtg gatcttctgc ccctaagaga cgatgaagcc aacatcttgt
     5101 ttgaagtggg catcagactt ggtaggggcc tcgaccgaga gcagatcgca ctcatactaa
     5161 aagccgtttc gcgtctggaa gaaagcaggc agacttgtag agaaagactc aaacgggttt
     5221 caccactttg gcggtggctt tcgccgcttg cggcgcttct tatcgtactt ctttcatgct
     5281 aggaggaagg ggcagtgcag attggcgtaa tcatgcagct tgtgggtctt ggcatcatcg
     5341 cagcggtagt gcacatagtt ttgaagcagg ccgggaaaga agacatcgcc ctgattgtca
     5401 ccatcggtgc ggtagtaatc ggcttcattc tcatcttaga tgaaatcagc aggcttttca
     5461 gcagcatccg tctgctgttc gggctctaaa ggggaggata ccatggccat ctggcaggtg
     5521 gtaggtgttg gcatcatcac acttgttgca tctttggttc taaagaagta cctagaagag
     5581 gccagtctgc cggtgctcct tgcaggtctc ctagtgattt tgggcttaat ctttgagccc
     5641 ctaaagcttc tctgggagac ttttcgagag tttggccaca tatcggggat taactttgca
     5701 tatcttgaac tgatcggcaa gatcctgggc accgctttcc tggcaggtct tgcagcggac
     5761 atctgcagag actgcgggga gagtgctctt gcggcaaaaa tcgagcttgc aggcagaatc
     5821 attatcatcg tcctcactct gccggtcctg caggcggttt tgacaggcct tttagaggta
     5881 ctaccgagat gaagtggata gcgcttctcc tagtggtttt gtcagctatg tcttctgccg
     5941 ctgccctgga tactctagag atccccaccg aactggaaag acttctcccg gacaccattg
     6001 agccttactc cattgaagag tttttgcggg acccctctgc agaaaaagtc ctgagcactc
     6061 tctggcagta cctctggaag gaagttaggt tacatgccag gcttatgggc cagatcattc
     6121 tagttgctgc cattgcagca gttctcaaag agatcggcac ttccctaagc aaagcagctg
     6181 ctgaagctgg gtttcttgcc acctacctca tgttggtgct ccttgttgtt gccagtttga
     6241 aagaagctgc aaacctcact acttcaacta tcgtgctcct tgcctctgca gtccaggttg
     6301 cagttcctct ggtggggctt ttccttgcag ctggaggcga actggtgaca aacgccactt
     6361 taagcccgct aatcctcggc ctaaccgctg tggtaagcgt tgtggtacag agagtggtga
     6421 tgcctttcgc cctggcatca ggagctatcg gacttgccag tagcgttgca gaaaagcccc
     6481 gcttgctgag gctttcggga ttcttccgct ggctgggaac cttgaccttg ggctttgtga
     6541 atgcggtgtt tttcggcctg attacctctc taggccttgg cagtgccgct caggacagcc
     6601 tagcccttcg cactgcaaaa tggttgatgg gaactgtacc tgtggtgggg gggcagatcg
     6661 caggcactac cgaccttctg caggcgtcta ccagagccat tcagtctgca gcctctacag
     6721 tcggactctt gttcgtcttc tttgccacct tgtttccagt gctcaggctg gcagcactgg
     6781 ttcttgccta caagctcaca acggcagtag ttgaagctgt ggcagagacg cggtttgtaa
     6841 gcgcccttga gactatggaa aaaaccctga cgtttttcct cggccttatt gtgctgaccg
     6901 cagtgatgtt ttacatcggt cttggcaccc ttgctgcact aggcgccctg atcttgaggt
     6961 gaccgtaatg aacgaaatct ggcagactct ggcaaaactc atcggactgt gctttttgga
     7021 agagatgagc tgcttgttta ttcacgatga aaaactggga aagaccgtta ggctcgtctt
     7081 cgggctcttg tttcttgcgg tgcttcttgg cccgctcctg aaccttttcc aaatcggcga
     7141 cctgctttcc cagatcgact ttgaggggtt gaccaagtaa tgctcgaaaa gatcaaagag
     7201 gttttgcaga aaattccgtg gccgcttttt gtcgttggat ttgtgggggt agttctgctc
     7261 atctggggta gccttccgga gaaaaagccc gctcctgtca tagaagccac tctggaaaga
     7321 ccccagatcg gtgaggagga aaagcttgct cagcttttgt cgtcgttggg gatcggtagc
     7381 gtcaaggtga tgatcaccta tgaagacgaa ggcaaaactc acgtggcaga agacaaaagc
     7441 gtagaaacca catacgacgg cagtggagtg cgttcggtga aagaaagcac cgaagtggtg
     7501 cgaatgaaaa ccgcctccgg cgagacgggg cttgtcttgg ttcaggagtc cccaaaggtg
     7561 agcggtgttg tggtgaccac ttccaaacca ttatccacag ctcagaaact gaggatcatc
     7621 gaggcgatcc gtgctttgtg ggatttacct ctaggaagga tagtagttct cggaaaggaa
     7681 tgagcgtttg atgaagaggg caatcggttg gtttgttttg ctgcttgttc tcagtacagg
     7741 tattacggtg tggtggaact ttggcgggag agaagccgtg tcgagtctaa ggttttccct
     7801 tccggttttt ggcggaaggg ttgatgacat cgactacaaa aagtatgaga acttcgacat
     7861 tcccgaagac gatccgctgc ttttggatcc gaaggagatc tcctatgtcc gtgaagaaga
     7921 agaccctgaa gtagttgcgg tggtagcatc ctacgatcct ttgatcgaat acaggctggc
     7981 tcgagatatt gccagggaaa acgctaggat tggtttggaa aggctggcga attctggaag
     8041 cgtcgatgcc tctattgagc ttctcaacca aaccagaaat gtcagtaaag aagaggaact
     8101 agagagcatt ttgaaagcca agggctttgg cgacacggtg attaccattg aggacaacat
     8161 ggtaaaggtc attgccaaag acctgaacaa gcaaaaagtc gaggcaatcg ccgagattat
     8221 ctacaacttg acaacctttg gcaaggatca aatcgttctt accagcattt agaattactg
     8281 gaaacaggag gaaaagcttc ccttataacg aacttatata tgtagctact aacactaact
     8341 ttcccgtaac tgttttggta taataatgtc tactagtctt agatacgaaa gcaatccggg
     8401 cttttcgcca acactagtta taagggggct aacttatgga tttaagagac gttagagatc
     8461 tcatcaaact tcttgacgaa agtaacctga ccaaacttga gattgaagat ccaaatggat
     8521 ttaagcttaa actggaaaaa aactgctcgg aagcggcgtc taagtccgtt cccgttagag
     8581 gcgcagagtc cgttcttgag gatgagcaga agctcgataa cacgatctcc gcaagggatt
     8641 tagatcagga gctcaaaacc ataacttcac cgatggtcgg gactttctac agagccccgg
     8701 ctcctgacgc tgaaccgttt gtcaaggaag gttcaacggt ggacgtaggc gacacgctct
     8761 gcatcatcga ggccatgaag ctgatgaacg agatcacttc cgaataccgc ggaagaatcg
     8821 tgaagatcct cgtggaaaac gggcagccta tagagtacaa ccagccactg ttcttgctcg
     8881 agagagtgta aggggggtgg ctgtgattgt ttagaaaaat cctcgttgcc aaccggggag
     8941 agattgcact tcggatcatc cgctctgcca aggaaatggg cattaaaacg gtggccatct
     9001 actccgaggc agacaaggag tctctacacg tacagtttgc cgacgaagcc tattgtgtag
     9061 gccctgcaga gcagtccaaa agctacctga acatccccaa catcatcagc agtgccatcg
     9121 tctctggtgc tgatgccatt caccccggct acggtttttt cgcagagaac gccgaatttg
     9181 cagaagtctg tagcgacaac ggcttcaggt ttatcgggcc gagcaaagag gccattgccc
     9241 tgttaggcga caaagctcaa gctaaaaacc tcatgcagga agccggtgta ccggtgatcc
     9301 ccggctcgcc aggctgcatc tgcagcttag aggaggccat ggcttttgcc agggaagtgg
     9361 gttacccggt tatcgttaag gcggcgcaag gtggcggagg ccgaggtatg cgggttgcaa
     9421 gaaacgaagg cgagttgaaa aaagcttacg atcttgcacg caccgaagca gaaaaggctt
     9481 ttgaatcagg cgaagtctac ctggaaaaat acgtggaaaa tcctaggcac attgaagtgc
     9541 agattctcat tgatgaactg ggaaacggca tctacctagg ggaaagagag tgttccctgc
     9601 agagaaggca ccaaaaagta ttggaggagt cgccttctgt ggcagtgtcg ccggaactca
     9661 ggcagaagat gggcgaagct gcagtgcggg gcgcgctggc tgcaggatat accaatgcgg
     9721 gaaccatgga gtttcttcta gactcccaag ggaacttcta ctttatggag atgaacaccc
     9781 gtatccaggt ggagcatccg gtgaccgaga tggtgacagg cgtcgacctc atcaaagagc
     9841 agataaaaat cgccgcaggt cagcctcttt ctctgaagca ggaagatgtt gtgattaaag
     9901 ggcacgctat agaatgccgg atcaatgctg aagaccccga caagggcttc agacctgctc
     9961 ccggaccgat tcagggattt cttcttccag gtggagctgg catcagggtg gatacggcag
    10021 cttacgccgg ctacgaaatt ccgccttact acgattcgtt gtttgccaaa ctcatagcat
    10081 ggggtaatga tagagaagag gcaatatgtc gaatgaggcg ggcccttgat gagtttatcg
    10141 ttgaaggcat tcccaccacg atcccgtttc acaaaaaagt tctggaaaac gaacattttt
    10201 tgcagggaga tatagatacc gcgtttattg caaagcatat actcaaggac cactaattgt
    10261 cattttgctt ccgaattgat atacttttaa gtgaattagg tcgggaggtg tatattgatg
    10321 ggtgaacaga ttttggtgaa tactgaaaga tccgaccttg gccaagtgcg tattgccgat
    10381 gaagtcgttg gcattattgc aggccttgca gccagcgagg tagaaggcgt acacagcatg
    10441 agcggtggta taaccggcgg aatctacgac atgttcggac gcaagaactt ctccaaagga
    10501 gtcaaggttg aagtcggcca agtagaagta gctgttgata ttgcaatctt cttggagtac
    10561 ggctacagag ttcctgatgt agcaacaaga gtgcaggaaa acgtcaagaa agcaatcgaa
    10621 agcatgacgg gattgacggt cgtggagata aatgtccatg ttcagggtat tcatttcccc
    10681 cagacccaag tgcctgagga aaaggaaact agagtaaaat agacgcaaat acccctcccg
    10741 ttcgggaggg gtaaggcact ttaggttggg acaagcttgg aagttcaaag accttgatcg
    10801 catcttgcaa gttgggagga aagcagatga attggcttga caaagtcctc atttttgtgg
    10861 cgtctcttgt gctggcaatt ttaggtatta ccctgatctt attggggctc ggtgtggatt
    10921 tctggaattc ttttgctggt gcgcacgact acctggccac aaacgcgctg gtcagcttcc
    10981 ttgtgggagc agtgctctgt ataatcgggt tctaccttgc aggtaacttg ctcggagaaa
    11041 aagaggaaga ggttggcatt gttgccgaaa acgagatcgg taaggtcaat attgcggaac
    11101 gtgttattaa agcctacgcc gagcgcgctg ctcaaagtgt cgaaggcgta actggtgtga
    11161 agtcgaagat caagtctcaa gcagagggac tgtccatcgt tctggatctt tccaccaaca
    11221 ccagcgtcaa gactgcagac actgcaaagc atgtccagga gagggttatg gaatacgtct
    11281 ccgattctct cggtactaaa gttacaagcg ttgaagtaat tattagcagt gttgaggaga
    11341 gcaaaccgca cagagtcagc taaaaggttt tgtttggggg cttagttaga tgtcaattat
    11401 caaagagata ttcgatcgat tgtctcccta taaaggcaga attattggcg tgcttttcgg
    11461 cttgattttg gcaatacttg ttctaagcat aggttttctg agagctctgg tcgtcggact
    11521 ttttgtggcc ctcgggtttt acttggggaa aaaggccgat aatcaagaga gcctcgttga
    11581 tttgtgggaa aggttgagga gaaatcgcaa tggatgaaag cacaaaaaga cgcacaaaga
    11641 gagagcgagc agttgaactg ctctaccagc tagaactcac cggcaaaagc cttgatgaag
    11701 tctttagcta tgccgaccca cctgttttag aagacgaacg agcgctgatc gttggagtat
    11761 gggagaagcg ccatgagatc gacgagatga tctctagaac cagccgcgaa tggaagctcg
    11821 atcggatcgg gtatgtggag cggaatattc tgcgtcttgc tctttacgag ataaattacg
    11881 acgatgtagc ggttccttac gctatcaacg ccgcagtgga acttgccaaa gagtacagcg
    11941 gcgaagaggc tgggagattt gtcaacggag ttttaggacg agcagtccgt gagaaaagtg
    12001 aataagccgg caagttataa gaaaggcttt ctcggcatag atacaagtaa ctaccgcacc
    12061 agctgctgcc ttttggatga agctggaagg gtagtctatc agcgccgccc cttgctctta
    12121 gtgcctcagg gtaagctggg gttaagacag caggaggcgg tttttcagca tttaaaagcc
    12181 cttcctctga tgtttgaaga aatggggcca ttagcagatg tgcagtttgg cgtgaccaca
    12241 agaccgagac ctgttgaagg gagctatatg ccctgttttg tagtgggaga gtcctttgta
    12301 cgagctttag ctgcaggcag ggggtatctt gccatcagcc accaggaaaa ccacctatgg
    12361 gctggtcttt acgaaaaccg taccctaatc gggaaaccct tcattgcggt tcacctttcc
    12421 ggaggaacca ccgagtttct ccatgtcaca tggaatgacc agaatctcca aatcaaagag
    12481 gtaggccaga ccactgatat cagcgccgga cagttccttg acagaaccgg tgtccagctg
    12541 ggatttccct ttcccgcagg cccttacctt gaaaagatcg cccgacaatc ggatctgaga
    12601 gttggagtca ccgtgaaggg caccgccatt tcctactccg gtcct
//
LOCUS       DAOSDA010000004        11578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_3724_length_11578_cov_3.315196, whole genome shotgun sequence.
ACCESSION   DAOSDA010000004 DAOSDA010000000
VERSION     DAOSDA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11578
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3724_length_11578_cov_3.315196"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLW82_00240"
     CDS             complement(<1..676)
                     /locus_tag="PLW82_00240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ45739.1"
                     /translation="MKIGIFSDTYYPQINGVATSVLLLKEYLEKKGHEVYVFTTTDPK
                     APVHEERVARYPSIPFSNTGRRIAAVLYPAIERAFKRNDFDIIHTHTEFSIGILGRRL
                     ARSLEIPHVHTMHTIYEDYTHYIARWKVLTPVNRALARKYTASFCNSADRVIVPTEKT
                     KELLLSYGVSRKIEVIPTGLRLERFAKSAWKPEEVEKAREGLGLGKNDMAVIYIGRVS
                     KEKNLDE"
     gene            1069..2817
                     /locus_tag="PLW82_00245"
     CDS             1069..2817
                     /locus_tag="PLW82_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ45740.1"
                     /translation="MRLIKRFISYYKYELPLTLLDIFAASGIAVLDLLFPMITRKMMK
                     DFIPALDVASIFRYGLVLLLMYFVVLILHYIVDNYGHVAGMRIETRMRQDLFTHMQSL
                     DVEFFDNNRTGSLMSRIMNDLHEVSELAHHGPEDLLISAVMLVGATIYLMRINVPLTL
                     LIVAFVPLMIFFAIKTRKKMARAFSSIKEQTAVINSDIENSISGIRVAKSFANEDYEF
                     ERFNKANHSYRQSREGAYHHMAVFYSGLNFMTNLLNGAVLVFGGYLTYLGKIDLADLT
                     AYILFVSMFIQPIRKLTNFAQQYEQGMAGFKRFAELMETKPKIVDGENAVNLTNVKGR
                     VTFENVSFSYTGHAEVLRDISLEVEPGKTIALVGPSGGGKTTLCHLLLRFYQPQDGSI
                     KIDGIDLRDIKLSSLRQTVGLVQQDVFLFAGTIRDNILYGRPDATEKEIVEAAKKAEI
                     HDFISSLPNGYDTYVGERGIKLSGGQKQRIAIARLFLKNPPILILDEATSALDNVTEY
                     RIQDSLKKLSEGRTVFLIAHRLSTVKDADSIVVLEEGKIVEQGTHAELLKTSGVYAEL
                     YGSQHVADPEKAFLLI"
     gene            complement(3558..3731)
                     /locus_tag="PLW82_00250"
     CDS             complement(3558..3731)
                     /locus_tag="PLW82_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45741.1"
                     /translation="MLKKLRLALWLFVFLSVIAFGSEIWFRGFDLTSVSMDEIIDRES
                     AELKTVEQGIKEA"
     gene            complement(4429..5577)
                     /locus_tag="PLW82_00255"
     CDS             complement(4429..5577)
                     /locus_tag="PLW82_00255"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOJ45742.1"
                     /translation="MGRFIVWVLDSVGIGEMPDSYLYNDQGSATLQNIARERGGLYLP
                     NLERLGLGLIEDIFGVGRPGDPQAAYGKMAEQSKGKDTTTGHWEIAGLHLEMPFPTYP
                     NGFPEEVISRFEKEAGVKVLGNYPASGTVIIEELGDEHMRTQRPIVYTSADSVFQIAA
                     HIDVIPLERLYELCEIARGILVGEHGVARVIARPFAGEPGSFYRTKDRRDYSLPPTGE
                     TILDVLVASGVPVTAIGKISDIFTGRGISTSLSAKSNNETMSQLLFALDTVEEGLIFA
                     NSVDFDMVYGHRNDVEGYAQALEETDRYIPDVLRKLRIDDILVITADHGCDPTTQSTD
                     HSREYVPLLVYGIKPRNLGIRETFADVAQSIAEYFGVSYPRGKSFLGE"
     gene            complement(5630..6529)
                     /gene="xerD"
                     /locus_tag="PLW82_00260"
     CDS             complement(5630..6529)
                     /gene="xerD"
                     /locus_tag="PLW82_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOJ45743.1"
                     /translation="MWEGAISEYLNYLLVERGMAENTITSYRKDLQQFAKIMKEAGIA
                     DPLRVSEDDLYLYLAVLWKKMVTSTVARKVACIRGFFEYFRGNGKIKVNPAKNLEGIK
                     RRQHLPEFLSEEEVDRLLSIPGGDVLGIRDKAMLELMYATGMRVSELLSLNIGDIEFE
                     AGYVRTRGKGDKERIIPLGEVANEALQNYLRVSRPRLLKTPTDAFFVNARGDRLTRQG
                     FWKILKSYTEQAGIKKKISPHSLRHSFATHLLKNGADLRTVQELLGHADIATTQIYTH
                     LSQSRLRQVYVETHPRGKKRNLH"
     gene            complement(6549..7175)
                     /gene="spoIIM"
                     /locus_tag="PLW82_00265"
     CDS             complement(6549..7175)
                     /gene="spoIIM"
                     /locus_tag="PLW82_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOJ45744.1"
                     /translation="MRSKLGKKAFSTEVLWPYLLISMMLVLGLLFGVLATRVVHSDQR
                     IQLRMYLDSYIGFIGKERGLDKASSALLKEALNLNLLKTAAVMVLASLSIVGVPLIAV
                     IAFLRGFILGFGAGFLIQELHLKGGLIFLAGLLPQNLFIFPGLVILGGASVVFSAAIC
                     STLFGRKKRSLMQEVQEYFTKTAVGVLVILLGCFMEAFITPGFLKLLV"
     gene            complement(7233..8393)
                     /locus_tag="PLW82_00270"
     CDS             complement(7233..8393)
                     /locus_tag="PLW82_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HOJ45745.1"
                     /translation="MQLVCLPEKVLDMEIYADLHVHIGRSKGEAVKITASRDLTLQNA
                     LNYAKKVKGLDALGIVDCGSEAVLAEITELIGAGVLKEAAGGGLITDDGLLLVLGWEV
                     ELGDVHYLIYLPTIEALTELAKTLWPRTKNPRLSTQRFAISPQELRSVAESLGGLFFP
                     AHAFTPHKGFYGRLVDSLYEVFDSPFVAIEMGLSSSTEMIAGIRELDPTTFVANSDAH
                     SLQKIAREFNQLEVECLSFAGIKEALEKEKVIANYGLDPRLGKYFESGCLRCGMPGET
                     GSICSTCGGPIVAGVRPRLEELGGFGEGRNRPLYVKQIPLEMLPYIGPRTLEKLQALG
                     TEIAVLHKLPIEQIATATNPQVAEVISQARLTGEKKLKISFGKAGYYGRVEL"
     gene            complement(8371..>9151)
                     /locus_tag="PLW82_00275"
     CDS             complement(8371..>9151)
                     /locus_tag="PLW82_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HOJ45746.1"
                     /translation="NNLPREIKGKGHIIKLRYTPLQLKVYAEEEKQEMPKEVCCPVIL
                     AELHSMLLPACYGVWAKNPKLTVGYIMTDGGALPIDYSETVKTLKGSGYRFITYTAGH
                     AFGGDREAVNIYSALALASMECDVLIAGMGPGVVGTGSTYGTTAIEVGTGINAAASLG
                     GRPIVIPRIMQYDRRGRHQGLSHHTLTSLKIALAPAEVVFTHKRWADLDLRPHKVVYW
                     GFPHMDYGAFALRTMGRGYPEEAEFFQTAAAAGSYAASMLT"
     assembly_gap    9152..9161
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9162..9381)
                     /locus_tag="PLW82_00280"
     CDS             complement(<9162..9381)
                     /locus_tag="PLW82_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HOJ45747.1"
                     /translation="MEGSIVEIGLRLGKVTEVGGEGAILKVKVETDIGERASLVYTNL
                     TGPVAVGDTVVINTTAVDLNLGSGGADFV"
     gene            complement(9436..11541)
                     /gene="pnp"
                     /locus_tag="PLW82_00285"
     CDS             complement(9436..11541)
                     /gene="pnp"
                     /locus_tag="PLW82_00285"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOJ45748.1"
                     /translation="MARKTYTIDLEGKSLTAEFGNYAAQANGSVMVYFGDTVVLVTAC
                     MSKEPREGIDFFPLLVDYEEKLYAVGKIPGSWGRREGKPTETAVLNARLIDRPIRPLF
                     PDGFRNDVQIVATVLSVDNDYAPEVAATIGASLALLVSDIPFDLPMAGVRVGLADGKF
                     LLNPTIKEQENCDMYIMVSGTSEAITMVEAGASEISEEEALESIFFAHEYIKKICAWQ
                     ETIRQEIGKPKLEVPLATPDVQLQERIKELGLSKMSTALRVFDKQERAAAVDAVRAEI
                     LEDLLKEMGEEAFAEKEKEINSLIDELEANEVRRMITEEKIRPDGRALTEIRPIRCDV
                     GILPRAHGTGMFHRGQTQVLSVCTLGALHDVQLLDGLTEEESKRYIHHYNFPGFSVGE
                     VRPMRTPGRREIGHGALAERALLPVIPAEEEFPYTIRVVSEVLESNGSSSMASVCGSS
                     LALMDAGVPIKKPVAGIAMGLIKGQNDFTILTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKVTGVSREILKAALAQAKEARLFILDKMAEVIAEPRPELSPYAPRMVTMQIDPD
                     KIRDVIGTGGKTIRSIITETGVEIDIKDDGTVYIASVDLEAANKAVKRITELTKEVEV
                     GEVYEGTVTRIAKFGAFVEILPGKEGLIHISKLAKERVANVEDVVKVGDVVQVKVIEI
                     DQQGRINLSRRALLEPESK"
BASE COUNT         2824 a         3113 c         2633 g         2998 t
ORIGIN      
        1 tctcatccaa gttcttttct ttggagactc tgccaatgta gatcacggcc atatcgtttt
       61 tcccaaggcc cagtccttct cgggcttttt ctacttcctc cggcttccac gcagactttg
      121 caaacctttc caaccttaaa cccgttggaa tgacctctat cttccgagaa acaccgtagc
      181 tcagcaagag ctctttggtt ttttctgtag gcacaataac tctgtctgca gaattgcaga
      241 acgacgcggt atacttccgc gccaaagccc ggtttaccgg agtgagaact ttccacctgg
      301 caatgtagtg ggtgtaatcc tcgtagatgg tgtgcatggt atgtacgtga ggtatctcca
      361 gagacctcgc caacctccgt cctaaaatgc cgatggaaaa ctcagtgtga gtatggataa
      421 tatcgaaatc gttgcgctta aaagccctct cgatggcggg atacagcact gctgcaatgc
      481 gtcttcctgt gttggaaaac ggaatgctgg gatagcgcgc cacccgctct tcatgcacag
      541 gagccttcgg atcggttgtt gtaaagacgt acacttcatg cccctttttc tctagatact
      601 ccttgagcaa aagaacgcta gttgcaactc cgttaatctg cggatagtaa gtatcgctga
      661 aaatccctat tttcatcctc aatcccctac tcgcgtcata ttttgcaaaa atgatcgccg
      721 tatgcagagc ctcctctgca aagcacggga cttgtccccc ctacaagttc ttcgcctgtt
      781 ttccaatggg caagctgcct tgaaaagaca tgcgtcaagc tagaggctcc ttttttacac
      841 cttctatctg catcatacca caggtctcaa ggcaggtagt agagtctttt ccatctaaac
      901 gaaaagaaga cctttccttc atcgtgcgca gtcgcaagga ctaaacacaa ccgatgaaga
      961 agagtaatct aaacagagcg ccatgccgtt caatggtgtg ggtttggcct gcaccgtttt
     1021 ttttgaccta gatcgaacta tcaagatgca agaaaggaag tccgccgcat gagactcatt
     1081 aaacgcttta tcagttacta caagtacgag cttccgctca ccttgcttga catcttcgct
     1141 gcatctggga ttgcagtgct cgacctgctc tttcccatga tcaccaggaa aatgatgaaa
     1201 gacttcattc cagctctgga tgtcgccagc atcttcaggt acggtttagt cctgctccta
     1261 atgtattttg tcgtacttat tctgcactac atcgttgaca actacggtca cgttgcgggc
     1321 atgcgcattg aaaccagaat gcgccaggat ctcttcaccc acatgcagag cctggacgtg
     1381 gaatttttcg acaacaaccg caccggaagc ttgatgtcca ggatcatgaa cgacctccac
     1441 gaggtgagcg aactggccca ccacggtccg gaggatctgc tgatctcagc tgtaatgctg
     1501 gtcggtgcca ccatctacct catgagaatc aacgttccac tgactctgtt gatcgtcgca
     1561 ttcgtaccgc tgatgatctt tttcgccatc aaaaccagga aaaagatggc aagggccttt
     1621 tctagtatca aagagcaaac tgcggtcatc aactcggata tcgaaaacag cattagcgga
     1681 atcagagtgg caaaaagctt tgccaatgaa gactacgagt tcgaacgctt caataaggca
     1741 aaccactcct accgccagtc gcgggaaggg gcctaccacc acatggcggt gttctattcg
     1801 gggctcaact tcatgaccaa tctcttaaac ggagcggtcc tggtgttcgg cggttacttg
     1861 acgtacctcg gcaaaatcga tctggccgac cttaccgcct acattctctt tgtttccatg
     1921 ttcattcagc ccatccgcaa gctgaccaac ttcgcccagc agtacgaaca gggtatggcc
     1981 ggcttcaagc ggttcgcaga gctcatggaa acgaaaccca agatcgttga cggcgagaat
     2041 gcagttaatc tcacaaacgt aaaaggcagg gtaacctttg aaaacgtgtc cttcagctac
     2101 acaggccacg ctgaagtcct gagagatatc tccctggaag tggagcctgg caagaccatc
     2161 gctcttgtag ggccttcagg aggaggtaaa accaccctct gccatctatt gcttcggttc
     2221 taccaaccgc aggatggcag cattaaaatc gacggcatcg acctgaggga tattaagctc
     2281 tcatccctcc ggcagaccgt aggtctggtc caacaggatg tcttcctctt tgcgggaacc
     2341 attcgggaca atatcctcta cggcaggccc gatgccacag aaaaagagat tgtggaggca
     2401 gcgaagaagg cggagatcca cgacttcatc tctagcttgc ccaatggcta cgatacctac
     2461 gttggagagc ggggcattaa gctttccggc gggcagaagc agcggatcgc tatcgctcgt
     2521 ttgttcctga aaaacccgcc catactgatt ctcgatgaag caacatctgc cctggataat
     2581 gtcaccgagt accgcattca ggactcgctc aaaaagctca gtgaaggcag aaccgtcttc
     2641 ctcatcgccc acaggctttc cacggtgaaa gacgcagact ccatcgtagt tcttgaagaa
     2701 ggcaagatcg tggaacaagg aactcatgcg gagctcctaa agaccagtgg cgtctacgcc
     2761 gaactttacg gctcccagca cgtggcagat ccggaaaaag ctttccttct tatctaaaac
     2821 ccgagactca aacgcaagca gtagaatgat gctaccggtc aagtgggcaa aaaaccaggt
     2881 catagcttac accagccaca tcaactgagg caagaacgcg ggttgtgatg tcaagtctct
     2941 gaaaaactcg gacatccgca tgtaacaaaa ctgggcgtag tactctcacg cccaaagcat
     3001 atagaaacct ttttgcttcc aatcggatgg aaaacgtcca taaacgttga tagatcaacc
     3061 gtttatggac gttttattaa actacaacac agaaacacgt tgctagagca aaaagaatcc
     3121 taaaacacat catctacttc aactaaagtt gtgccgaaca actcaagtac caattcgtga
     3181 aacttcgtgt ctataatcct ttccactacc ggtaggataa tataagagat ttccaacttc
     3241 gaagatgacg gtaacttctc caacacgaca agtaaatcgc cttgtttaaa ctgaactatt
     3301 ccatcaccaa catcagttgg agatccgtac ttctaagaaa gttgatacga gagggctgaa
     3361 aacgctactt cactgtgtgt ctgtgaagtt attaaacctc tgtagaacct gtacttcaca
     3421 tagaacagtt cacgttcata aaagctgtat tctaattcta cgggcaaatc caaaattgat
     3481 gtttcaaatg taactctgtc aacatcaagc gagaccctta acccaccaat aggagtatca
     3541 ggaagtatat ctgaaaccta tgcttcttta atgccttgtt caactgtttt cagctcagca
     3601 gactctctgt cgattatttc atccattgag acggaagtca ggtcgaaacc tcgaaaccaa
     3661 atctcagacc cgaatgcaat tacggacaga aacacaaaaa gccacaaagc aagtctaagt
     3721 ttctttaaca tccgcataaa cctcccattg cttgttgacg tgtcctgtaa cacacttttc
     3781 aaactatccc ctgacgatgc cattaagttc gttttctgct gattcttgtt taagagagca
     3841 ccatatgtat tctacttctc agcaagattc gagatgactg gaacagcaaa cgattgaatt
     3901 agcgagttaa ggacttggct cacgctcctc aatcccctcc atgtaaaagt tcgaggaaac
     3961 gagagcctat caaacttttg taatggatcg tacgcaataa tgtgcttcta accttctgct
     4021 gcccactact atgccaacaa ccaaaactca cccccgatcc catttttttg atagttctat
     4081 attgattaca gtgtcccttc taatgatcgc cgaaaattcg gaatttgttg atatactcgc
     4141 aacaataaaa ccaattacgc tctgcagggt ggttttccga gaccggttga acggactcct
     4201 accgcacgat acaacttcct cccacttttg cagagcactt cctgattccc tcgttcggtc
     4261 tgcaacctct aaatgtgctc tatgtgtatg agtttcttgc tggctccttc gtcttcacta
     4321 ctagatcact ccttttggcc tgaggtgacg gcattaagta gcgatcaaaa aagaggcttg
     4381 cagcatcaat aaatgctgct cgcctctttt ctgcttcaaa ccttaaaact actccccaag
     4441 gaagctctta cctctggggt aggagacgcc aaagtactca gcgatagact gggcaacgtc
     4501 tgcaaaggtc tctctgatcc ccaggttccg cggcttgatc ccgtacacca aaaggggaac
     4561 gtattctctg gagtgatctg tgctctgagt tgttggatcg catccgtggt ctgcagtgat
     4621 caccaggata tcgtcgatcc tgagtttcct taaaacatcg ggaatgtagc ggtctgtttc
     4681 ttctagagcc tgagcatacc cttccacatc atttctgtgt ccgtagacca tgtcaaagtc
     4741 cacagagtta gcaaagatca aaccttcttc tacggtgtcc aaagcaaaaa gcagctggct
     4801 catagtctcg ttgttggatt ttgctgaaag ggacgttgat attccccttc cggtaaagat
     4861 gtcggagatc ttacctatgg ctgtaaccgg cactccactt gcaaccagca cgtcgaggat
     4921 ggtctctcct gttggcggca gggagtagtc cctgcggtct ttggtcctgt agaaactacc
     4981 gggttcacct gcaaaaggcc tggcgatgac tctggcaaca ccgtgctcgc caactagaat
     5041 gcccctggca atctcgcaga gctcatacag cctctctaag ggaatgacat caatgtgtgc
     5101 tgctatctga aaaaccgaat cggctgaggt gtagactatt ggcctttgag tccgcatatg
     5161 ctcgtcgcca agttcctcaa tgatcaccgt tcccgatgcg gggtagttgc caagcacttt
     5221 aacaccggcc tctttttcga accgggaaat cacttcttca gggaagccgt tcgggtaagt
     5281 cggaaacggc atttccagat gcaagcccgc tatctcccag tggccggtgg tcgtgtcctt
     5341 acctttggat tgctcggcca ttttgccgta tgcagcctgg ggatctccag gccttcccac
     5401 tccaaaaatg tcttcgatga ggcccaaacc taggcgctcc aaattcggca gatacagtcc
     5461 cccgcgttct ctagcgatgt tctgcagagt agcgcttccc tggtcgttgt acaaatagct
     5521 gtccggcatc tctccgatgc ccacgctgtc taaaacccaa acgatgaatc tgcccatgtt
     5581 caacctgcct ttctgcaaaa tcggtcttgc ttcaagttat ctgcaacagc tagtgcaagt
     5641 tccgcttttt ccctctggga tgagtctcga cgtagacctg acgcagcctg gactgactga
     5701 ggtgggtgta aatctgcgtg gtcgcaatgt cggcatgtcc caaaagctcc tgcaccgtgc
     5761 ggaggtctgc cccgtttttc aaaagatggg tggcaaacga atgccgcagc gaatgagggc
     5821 tgattttttt cttgattcca gcttgttctg tgtagctttt caggatcttc caaaaaccct
     5881 gcctggtcag cctgtcgcct ctggcattca caaaaaaagc gtctgtagga gtttttagga
     5941 gcctcggcct gctgaccctg aggtagttct gcagcgcttc attggccact tcgcctagag
     6001 gaatgatccg ctctttgtct ccctttcccc tagttcttac atacccagct tcgaactcaa
     6061 tatcaccgat gttcaagctg agaagctcgc tcaccctcat accggtggcg tacatcagct
     6121 ccagcatagc tttgtccctg atgcccaaca cgtcccctcc tggaatagac agaagcctgt
     6181 ccacttcttc ctcacttaaa aactctggaa ggtgttggcg ccttttgatc ccttccaaat
     6241 tcttagctgg attaactttg attttaccat ttcctcgaaa atattcaaag aagcccctga
     6301 tgcaggcgac ttttctagca actgttgatg tgaccatctt tttccacagc accgcaaggt
     6361 acaagtagag gtcgtcctcc gaaactcgaa ggggatcggc gatgcccgct tccttcatga
     6421 tcttggcaaa ctgctgcagg tccttcctgt agctggtgat ggtgttttcg gccatacccc
     6481 tctcaacaag caggtaatta aggtattcgg agattgcccc ttcccacatg tctaccacct
     6541 acctaatatt agaccaaaag cttgagaaat cccggtgtaa tgaacgcttc cataaagcaa
     6601 cccaagagaa taaccagaac tccaacggcg gttttagtaa agtactcctg cacttcctgc
     6661 attagggatc gcttcttcct gccaaagaga gtgctgcaga tcgcagcaga aaaaaccacc
     6721 gacgctcccc ccaagatcac aaggccaggg aagatgaaca ggttctgcgg aagaagccct
     6781 gcaagaaaga tcagtccacc tttgaggtgg agctcctgga ttaaaaaacc tgcgccaaag
     6841 cccaaaatga aacccctaag aaaagctatt actgctataa ggggcacgcc aactatcgaa
     6901 agagatgcca gaaccatgac cgctgcggtc ttgagtagat tcaagttcag cgcctctttc
     6961 agcagagcgg agctggcctt gtccagacct ctctccttcc cgatgaaccc gatgtagctg
     7021 tccagataca ttctaagctg gatgcgctgg tctgagtgta ctactctcgt tgccaagaca
     7081 ccgaaaagca gccccagaac gagcatcatg gaaatcagca ggtaaggcca gagcacttcg
     7141 gtgctgaaag ctttttttcc cagtttagac cgcatagtat cacctcaaga caacctatgc
     7201 ttgaggggtg aagtttagac tagggagaaa acctacagct ccactctgcc gtagtagccg
     7261 gctttgccaa aagagatttt cagcttcttc tcaccagtga gccttgcctg gctgatgacc
     7321 tctgcaactt gaggatttgt ggctgtggca atttgctcga tgggcagctt gtggagcact
     7381 gcaatctcgg ttcctagcgc ctgaagcttt tccagagtcc taggtccgat gtagggcaac
     7441 atctccaggg gaatctgttt cacatatagc ggtctattcc ggccttcgcc aaacccacct
     7501 agttcttcca ggcggggacg aacgcctgct acaatgggtc caccacaagt gctgcaaata
     7561 gagccagttt ctcccggcat gccgcacctc aggcagcccg actcaaagta cttacctagt
     7621 cttgggtcga gaccgtagtt ggcaattact ttttcttttt ccaatgcttc tttgattcct
     7681 gcaaaactca aacactctac ttccaattga ttgaactccc tcgctatctt ctgcagcgaa
     7741 tgggcatcgg agttagccac aaatgtagtg ggatctagtt ccctgatccc ggcaatcatc
     7801 tcagtagagc tagaaagccc catctcgatg gcgacaaaag gactgtcgaa gacttcgtac
     7861 agcgaatcga ctagtctccc gtagaagccc ttgtggggag tgaaagcatg ggcagggaaa
     7921 aaaagccctc cgagactctc tgcaacgctt ctcagttctt gaggagaaat ggcaaacctt
     7981 tgtgtggaaa gcctggggtt cttggttctc ggccagaggg tttttgctag ttccgtcaga
     8041 gcttcaattg tcgggaggta aatcaggtag tgcacgtccc caagttccac ttcccagcct
     8101 aaaaccagaa gaagaccgtc gtctgtgatt aagcctccac cggctgcctc tttaagcact
     8161 cctgcaccga tcagttctgt gatctctgca agcaccgctt cgctaccgca gtccacaatg
     8221 cccaaagcat caagcccttt aacctttttg gcgtagttca atgcgttttg tagcgttaga
     8281 tcgcgggatg cggtgatctt caccgcttcg cctttggatc ttcctatatg tacatgtaga
     8341 tcggcgtaaa tctccatatc aagcaccttc tcaggtaagc atactagctg catagctccc
     8401 agctgctgct gcagtttgaa agaactctgc ctcttccggg tagcccctgc ccatagtacg
     8461 gagggcaaac gctccgtagt ccatgtgggg gaacccccag tagaccactt tgtgagggcg
     8521 aaggtccaga tctgcccacc ttttgtgggt aaaaactact tcagcaggtg caagggcgat
     8581 cttcagcgat gtaagcgtgt ggtggctgag cccttggtga cgaccccttc tatcgtactg
     8641 cataattctg ggaatgacta tgggccgccc tccaaggctt gctgcggcgt taatcccggt
     8701 gcccacctct atggcggtgg tgccgtaggt actgcctgta ccgacaacgc caggtcccat
     8761 gcctgcgatc agcacatcgc attccattga ggccaaagcg agggcgctgt agatgttcac
     8821 cgcttcccgg tcgccgccaa aagcgtgacc tgctgtgtag gtgataaacc tgtaaccgct
     8881 ccctttaagg gtttttaccg tctcgctgta atcgataggc agggctcctc catcagtcat
     8941 gatgtagccc acagtgagct tcgggttttt cgcccaaacg ccatagcaag caggaagaag
     9001 catgctgtga agttctgcaa gtatcacagg acagcacact tctttgggca tctcttgctt
     9061 ttcctcttct gcatagactt ttagctgcag aggagtatag cgcaacttga tgatgtgtcc
     9121 cttacctttg atttcccttg gcaggttgtt tnnnnnnnnn naaacgaagt ccgctccgcc
     9181 acttccgaga ttgaggtcca cagcggtggt gttgatcacc actgtatcgc caactgcaac
     9241 gggacctgta aggttggtgt agaccagaga ggctcgctca ccgatatcgg tctctacttt
     9301 gaccttcaaa atggcaccct ctccgcctac ttccgtaacc ttccccaatc taagaccgat
     9361 ttccacaata gatccctcca cctctattgt gaacaaaaaa agggggagag taatctcgcc
     9421 cctggagcgt actatttact tgctttctgg ctctaacagg gctcttctag agagatttat
     9481 tctaccctgt tgatcgattt cgattacttt gacctgaact acatctccaa ctttgactac
     9541 gtcttcaacg tttgccaccc gctctttggc gagcttggag atgtgaatca aaccttcttt
     9601 acctggtaga atctctacaa aagctccgaa cttggcaatt cttgttactg ttccttcgta
     9661 aacttcaccg acttccactt ctttagtgag ctcggtgatg cgctttacgg ccttgttcgc
     9721 tgcttccaag tctacagaag caatataaac agtaccatca tctttaatgt cgatttccac
     9781 tccagtttca gtaatgatcg aacggatggt ctttccgcct gtaccgataa catcgcggat
     9841 cttgtcggga tcgatctgca tggtaaccat tctcggtgcg tatggcgaaa gctccggtct
     9901 gggttcggcg ataacttctg ccattttgtc caagatgaac agtctggctt ctttagcctg
     9961 tgctaatgcc gccttgagaa tctctctgct gactcccgtt accttgatat ccatctggag
    10021 agcggtgata ccgtctctgg ttccggcaac tttgaagtcc atatcgccaa agtgatcctc
    10081 gagaccttga atgtcggtga ggatggtaaa gtcgttttga cctttaatca gacccatagc
    10141 gattcctgca actggctttt tgatgggaac gcctgcatcc attagtgcga ggctcgatcc
    10201 gcaaacgctt gccatggaag aggaaccgtt agattcgaga acctcactca ccacgcgaat
    10261 ggtgtagggg aactcttcct ctgcaggaat tacaggaagt agtgcccttt cggcaagtgc
    10321 gccgtgaccg atttctctac ggcctggtgt acgcattgga cgtacctcac caacggaaaa
    10381 ccctgggaag ttgtagtggt gaatgtagcg tttgctctct tcttctgtca gtccgtcgag
    10441 aagctggaca tcgtgcagcg ctccgagagt acagactgaa agaacctggg tttgacctct
    10501 gtggaacatg cctgtcccat gagctctcgg caaaatgcct acgtcgcacc taatcggtct
    10561 gatttcagtg agtgctctgc cgtcgggacg gatcttttct tcagtgatca tgcgacgaac
    10621 ttcattggct tccaactcgt cgatgagaga attgatctcc ttctccttct cggcgaaggc
    10681 ttcttcaccc atctctttta gcagatcctc gagaatctct gccctgacag catccactgc
    10741 tgcggctctc tcctgcttgt cgaagacgcg gagagccgtg ctcattttgc tgagtccaag
    10801 ctccttaatt ctttcctgga gctgaacatc gggcgttgcc agcggaactt ccagcttggg
    10861 cttgccgatt tcctggcgaa tggtctcctg ccaagcgcag atctttttga tgtattcatg
    10921 ggcaaagaag atcgactcaa gggcttcttc ttcgctgatt tcgcttgctc cagcctctac
    10981 catggtgata gcttcgctgg ttccagaaac catgatatac atgtcgcagt tttcctgctc
    11041 tttaatggtg gggttgagga gaaacttgcc atctgcaagt ccaactctta cgccggccat
    11101 aggcaggtcg aaaggaatgt cgcttaccaa aagggcaaga gacgcaccga tggtagccgc
    11161 aacttccggc gcatagtcgt tatccacgga aagtacagta gcgacgattt gcacgtcgtt
    11221 tctgaaacca tccgggaaga gcggcctgat gggcctgtcg attaggcggg cgttcaagac
    11281 tgcggtctca gtcggttttc cttctcttct tccccacgaa ccggggattt tgcccacagc
    11341 atacagcttc tcttcgtagt ctacaagaag cgggaagaag tcgatgcctt ctcgtggctc
    11401 ctttgacata caggccgtca ccaagaccac tgtatcgcca aaatatacca ttacagaacc
    11461 gttggcctgt gctgcataat tgccgaattc agcggtaagc gacttgcctt ccaagtcaat
    11521 tgtgtaggtt tttcgtgcca ttaatttccc tcctgtgcta aaaaagggag cggtaccc
//
LOCUS       DAOSDA010000005        10388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_4240_length_10388_cov_3.062325, whole genome shotgun sequence.
ACCESSION   DAOSDA010000005 DAOSDA010000000
VERSION     DAOSDA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4240_length_10388_cov_3.062325"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PLW82_00290"
     CDS             <1..548
                     /locus_tag="PLW82_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOJ45749.1"
                     /translation="RDPKAFYDFYFHMLSEFANAKPNPAHLAIAKLEEAGFVKTVITQ
                     NIDELHQKAGSKKVLEVHGTTFAGYCLDCKKESPLAYSTEVPRCSCGGIIRPKVVLFG
                     DGMPDAYFKAEEEAHKADLVLVVGSSLTVSPACYLPLRAKEMAIINLEPTPFDSRAIF
                     VSHEKAEEELPRLLQCLDLAG"
     gene            618..1436
                     /locus_tag="PLW82_00295"
     CDS             618..1436
                     /locus_tag="PLW82_00295"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOJ45750.1"
                     /translation="MKKLLGAFLIVALTAFAGRFFFSPDSQKPAPVPAAEIMTQEATL
                     TVATYNIHHGADQTGALRLREIGTLLQDADIVFLSEVDRRYSTRSGFADQLLLLKEYT
                     GLSYHAYAPTLQRAGFLNLGGSYGIAVLSRYPITNAQVIYLPTSAFAEPRAALVVEVN
                     WNGRTVRVLGTHLAVQENDRNQQLAALARLQLDLDLVLGDLNAAPKSIASLADHFQLT
                     PANNQPTFPAENPSVQIDYIFYSSRFDLLSVHTSSYLYSDHLPVIAKLKPNYLN"
     gene            complement(1970..3232)
                     /locus_tag="PLW82_00300"
     CDS             complement(1970..3232)
                     /locus_tag="PLW82_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45751.1"
                     /translation="MRKKALFWFFIFVLAIVPIASAAYVDIYPNYVIIKDRWPAVIRS
                     GQTLQLEWELPESADLSSFAILDSDIDILSRIEAINKGSLVGKSVTVHTSAGSYTGVV
                     KEDGGRLVLINPRNDQIIAIINASAVVSIIPGDDTASKNTLKLEVKNSGSTPVEDVNV
                     RYFASGITWQVKYVLQVDGTEVVLKGEYEITNTTNTEIKDAVVRLIARQDPTYLYKTR
                     EVVLYAAAEASDAALPNVEHQGETAVYTLPGRITIPANSKLKLMLLKEDDLKLERTYK
                     LNYDMVTVEYKLEGISQALPAGQIQVFDGDILMSTQSIASIPQSGELVLNLGQTMDIA
                     GRTEQTNYTNVGELHTSEYAVTLKNNKDEKVTVEVVVNLPRDNARIVAQPGLEVKRTS
                     SYTAIIYVDVEAKTERTINYTVEYTTNR"
     gene            complement(3409..3978)
                     /locus_tag="PLW82_00305"
     CDS             complement(3409..3978)
                     /locus_tag="PLW82_00305"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOJ45752.1"
                     /translation="MGIIQALMARKWILYGFLAALILGIVYVVLPTKPNQTASAAETS
                     PNPGVFSGNLALDITVIDREGNEVSLSDFRGRPVFLNFFATWCGPCKYEMPFMEKIWR
                     EYQEMGSDLVYLVVDVAENQETVEKYYRDNFYTMPYFLDPNGKAFSKYLFRGIPGSIF
                     INRDGVIVARYNEALPEATIRKNIELITK"
     gene            complement(3996..4697)
                     /locus_tag="PLW82_00310"
     CDS             complement(3996..4697)
                     /locus_tag="PLW82_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOJ45753.1"
                     /translation="MEATQISIWLAFLAGLVSFFSPCVMPLIPAYIAYLAGVGAENRG
                     RLLRNSLGFVLGFTVVFVVLGASATLLGQFLLRHQFLLRKISGVIIIVFGLHLAGLIK
                     IGFLNFDTRKQVKTKEGKDAGFLQSFVFGLAFSSGWTPCIGPILGSILILASTTASLT
                     QGMFLLVIYAVGLGLPFVLTAVLIDRLQGRWSRLTKITVWLSRISGYLLIVLGILMYF
                     GVLQSLAGYLPAFGL"
     gene            complement(4700..5035)
                     /locus_tag="PLW82_00315"
     CDS             complement(4700..5035)
                     /locus_tag="PLW82_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45754.1"
                     /translation="MIISMSKKELGLLAVFLVLFAGSLYGIFRLEKTGDAIPVIAVEE
                     YLVHEGTTYQRTLSVVAVIDRKLTPMSAYYQGRPLYKETSAKVPDRLLWPLEEGVYAV
                     FVKVVRKGG"
     gene            complement(5200..5931)
                     /gene="nagB"
                     /locus_tag="PLW82_00320"
     CDS             complement(5200..5931)
                     /gene="nagB"
                     /locus_tag="PLW82_00320"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOJ45755.1"
                     /translation="MKYVISSTYDEMSIEAAKYVADAIKAKPDIVLGLPTGGTPVGMY
                     KELVRLHKEEGLDFSKVRTFNLDEYLGLDSDHPQSYRYFMETNLFSHVNIKKENIHFL
                     SGIAEDPNEECLRYEKAIADVGGIDLMILGIGTNGHIGFNEPANCLQANSHLVALAQS
                     TIEANARFFEKKEDVPTRAVTMGVGSILRSKEIILLASGKSKQDAIYGTTNGVVTLDL
                     PASLLQLHRDVTLFLDEEAAAKIKK"
     gene            6136..7275
                     /locus_tag="PLW82_00325"
     CDS             6136..7275
                     /locus_tag="PLW82_00325"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOJ45756.1"
                     /translation="MVKVGLEILLENELSTVSGKRVGLITTPTGVDSRLNSNVDLLLE
                     HGVRVTALFGPEHGVRGDAQAGESVQSGKDPKTGLPVYSLYGPTKKPTKEMLDDVDVL
                     IFDMQDVGCRYYTFLYTLAYAQEAAAEMGKEFVVLDRPNPLGGLTVEGNLVKGGFTSF
                     VGGYSLPIRYGLTIGEFSLYLERYHGVGKKPKVIMLSGWKRGMYFGETALPWVLPSPN
                     IPTVDTAFVYPGTCLFEGTNLSEGRGTTKPFEIIGAPWLDGFALADRLNSFELPGVIF
                     RPVFFTPTFSKHQGTGCQGVQLHITDYDNFAPVRTALFLLSEVFKYQESRFLETNGHC
                     FFDLIAGDKKVRENLTAKAGPQEIWEEMESENRSFVEKAKSILLY"
     gene            7293..8405
                     /locus_tag="PLW82_00330"
     CDS             7293..8405
                     /locus_tag="PLW82_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOJ45757.1"
                     /translation="MESLVYAEPASLGIDPEKFKEAEAVLKEEIEKGSAPGAVALVLY
                     QNKIAYHYAFGKRALVPEPEEITLDTIYDLASITKCVATTTACMMLMEQGLIGLEDPI
                     AKFFPDFAGKEEITIFHLLTHTSGLPAWLPLYQEARSKEDYRKALAESDLEHQPGAKV
                     VYSCLGFILLGLILEKVAGEELDAFLQKALFDPLGMEDTCFNPKKNFGPEKLLRVAPT
                     EATEDRGLLRGEVHDENAYCIGGVSGNAGLFSTAVDLAKFASMLLSGGVFQGRKILEQ
                     KTIELMTQNHTAPVTPNRGLGWLKKNPISSGGSLLTDASYGHTGFTGTSMWLDPVLNV
                     AIILLTNQVHPSRHQGGVAKLRSSFADAVVSALPKR"
     gene            complement(8480..9664)
                     /gene="trpB"
                     /locus_tag="PLW82_00335"
     CDS             complement(8480..9664)
                     /gene="trpB"
                     /locus_tag="PLW82_00335"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOJ45758.1"
                     /translation="MMLPDQKGYYGEFGGSFVPAEVQKVLDEIAQCYLECKDDPQFQK
                     EFEDLIRDYVGRPSVLYYAEKFSEKLGGAKVYFKREDLNHTGAHKINNTIGQVLLAKR
                     LGKKRIIAETGAGQHGVATATACALFDMECVIYMGEEDTKRQALNVFRMELLGAKVVP
                     VKSGERTLKEAVDEAFMDLVANYETTYYMIGSAVGPYPYPMIVRDFQSVIGREAREQI
                     LEREGRLPDYLVACVGGGSNAIGLFAPFFEDKEVKLIGVEPGGKGLDTPYHAATLTKG
                     KKGQIHGFKCYSLEDETGEPLPVYSIAAGLDYPGVGPEHSYYKEIGRAEYAVVTDQEA
                     LDAFKFLSKTEGIIPALESSHAVAHAMKLAPQLSKDQIIIVNLSGRGDKDVMQVAELL
                     NK"
     gene            9890..>10388
                     /locus_tag="PLW82_00340"
     CDS             9890..>10388
                     /locus_tag="PLW82_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HOJ45759.1"
                     /translation="MRRHLPFFLLLALLLLIIWDIPFVPKEIYEPHLESLYAADHEEE
                     NAVLPDAQTADVNPADNLIVSPLHLASFCPYCEAEPIIKPGDSSDDLPGILEFLNDRG
                     YYKGELTSHYTDEAVAAVKTFQLDNKLTVDGIIGAQTWQAIGSSFATLPMAKAKPETD
                     NLSIFV"
BASE COUNT         2608 a         2714 c         2406 g         2660 t
ORIGIN      
        1 accgagatcc caaagctttt tacgacttct atttccatat gctgtctgag tttgcaaacg
       61 caaagcccaa tccggcccat ttggccattg ccaagctgga agaagcaggt tttgtgaaga
      121 cggtaatcac gcagaacatc gatgagctcc accaaaaggc gggaagcaag aaggttttgg
      181 aggttcacgg cacaaccttt gcagggtact gtttggactg caaaaaggag tctccccttg
      241 cctactctac tgaagttcca aggtgcagct gcggaggaat catcaggcca aaggtggtac
      301 tttttggcga tggtatgcca gacgcctact ttaaagccga agaagaagcc cacaaagccg
      361 acttagttct cgtggtaggt tcgagcctta ctgtgagccc ggcctgctac ctacctctac
      421 gtgccaaaga gatggccatt attaacttag aacctactcc ttttgactca agggcaattt
      481 tcgtctccca cgaaaaagcc gaggaagaac tcccccgact attgcaatgc ctagatttgg
      541 caggataggc tgagcaggag gcacaagaag tttttgcgaa cttaaatatg gtactgaagc
      601 aaaggaagtg cctctaaatg aaaaaacttc taggtgcctt cttgatagtg gcattaaccg
      661 catttgccgg caggtttttc ttttcccccg acagccaaaa gcctgcacct gtgcctgcag
      721 cggagatcat gacacaagaa gcaaccctta ctgttgcaac gtataatatt catcacggtg
      781 ccgatcaaac gggagccctg cggctaagag agatcgggac actactccaa gatgcagaca
      841 tcgttttcct ttctgaagtg gatcggagat attccacaag atctggcttt gcagatcagc
      901 tgcttcttct gaaggagtac accggtctga gctatcatgc ctatgcccca acacttcaga
      961 gagcgggctt tttaaacctg ggcggttcct acggcatcgc agtgctcagc aggtacccca
     1021 tcacaaatgc ccaggtgatc tatcttccga ccagtgcgtt tgccgaaccc cgagctgctt
     1081 tggtggtaga ggttaactgg aacggtcgga ctgttcgggt cttgggaacc catttggccg
     1141 tacaggaaaa cgataggaac cagcagttgg cagcccttgc ccgccttcag ctggacttgg
     1201 acctagtgct cggcgaccta aacgccgcac cgaaaagcat tgcttcttta gccgatcatt
     1261 ttcaactaac cccggccaac aaccagccga cgtttcctgc tgaaaaccca tcagtacaaa
     1321 tcgactacat cttttacagc agccggttcg atctattaag cgtgcacaca tccagctacc
     1381 tttactcgga tcatctgccg gtaatagcta agctaaaacc aaattacctg aactagaaac
     1441 gctccgcatc tatatgagga gcgtttttta tcaggtttgc ttttaaggta agcgaagata
     1501 agaaccaaag cagctttgcc agctgccatc ggcgacgaac gttcttccag atccctttaa
     1561 ggtcccgaat tctgagactt gccctcggtt tggtttttca gtttcaacgg cgggtggttg
     1621 ccgacataag cgactggaaa ccgctacccg ttcagaaaga aggtgtaaat gtcgacatcc
     1681 acaatggtta gcctctacac ttcaacgcct tcaggaagat caacaacgta tgctcggtcg
     1741 tctgggcgta atccttacac ataaacagca tactgccatt tgaactggag tagcatctta
     1801 agcttaaaag aagagctaca aatgccagta ctagcaaacg cctcctcata ccccatgtcc
     1861 cttttatgtg tggggcactg ctggagcaaa tcccgccaag agcgaacttt cgatgtgaaa
     1921 acagcttgga atgctgaagt tctgcatcca agctgttttt tcgtgtaaat tacctgtttg
     1981 tggtgtactc gacagtatag ttgatggttc tttctgtctt tgcttctacg tctacataga
     2041 tgatggcggt gtagctggaa gtcctcttga cctcaagacc gggctgtgca actatgcgag
     2101 cgttatctct cgggaggtta accacaactt ctaccgtgac cttttcatct ttgttgtttt
     2161 taagcgttac ggcgtactca ctggtatgga gttctcctac gttggtgtag ttggtctgct
     2221 cggttctccc tgcaatgtcc atggtctggc ccaagttcaa aaccaactca ccagactgag
     2281 ggatacttgc tatgctctga gtggacatga gaatatcgcc atcgaaaact tgaatctgcc
     2341 ctgcaggaag tgcctggctg atgccttcga gtttgtactc tactgtaacc atgtcatagt
     2401 tcagtttgta cgttctctcc agcttaaggt catcttcttt gaggagcatc agcttgagct
     2461 tagagtttgc cggaatggtg attcttccgg gcagtgtgta caccgctgtc tccccttgat
     2521 gctccacgtt tggaagagca gcatctgatg cttctgcagc agcatacagg acgacttctc
     2581 tagttttgta aagatatgtc ggatcctggc gagcgatgag ccttactaca gcatctttga
     2641 tttcggtgtt tgtggtgttt gtgatttcgt attctccctt aagtacgact tctgtcccgt
     2701 ctacctgcag cacgtactta acctgccagg taatgcctga agcaaaatag cgtacgttta
     2761 catcttctac aggagtactt cctgagttct ttacctccag cttcaaagtg ttcttgctgg
     2821 ctgtgtcgtc accaggaatg atggagacta ctgcactagc gttgataatc gcgatgatct
     2881 gatcatttcg cggattgatg agaaccagtc tgcctccatc ctcctttaca acccccgtgt
     2941 acgatccagc tgaagtgtga accgtaacac ttttccctac caaagaccct ttattaatgg
     3001 cttctatccg tgaaagaatg tcgatgtcgg aatcgaggat ggcaaacgaa ctcaggtcag
     3061 cgctttccgg aagctcccac tccagctgta gggtctgtcc tgacctgata accgcaggcc
     3121 accgatcctt gatgattaca tagtttgggt atatatccac gtaagcggca gatgcaatcg
     3181 gtacaatcgc caaaacaaat atgaagaacc aaaacagagc ttttttccgc atctaagatc
     3241 acctctctaa aattgttatc tacttccaga atactacctg caaaatccgg aagcagaacc
     3301 gtcttttatt gtattttcct ttctcgctta ttagacgaac ctctgagctt tttcgctccc
     3361 aaaagcaaaa aacagatttt agcatcagcc aaaatctgtt ttataagctt acttggttat
     3421 gagttcgata ttcttgcgga tggtagcttc cggcagcgct tcgttgtagc gggctacgat
     3481 cacaccatct ctgttgatga agatgcttcc gggtatgcct ctgaaaaggt atttagaaaa
     3541 agccttgccg tttggatcca ggaaatacgg catggtatag aagttgtccc ggtagtactt
     3601 ctccaccgtc tcttggtttt ctgctacatc caccactagg taaaccaagt ccgatcccat
     3661 ctcctgatac tccctccaga tcttctccat aaagggcatc tcatacttgc agggaccgca
     3721 ccaggtggca aaaaagttca ggaagaccgg ccttccccga aagtcgctga gggagacttc
     3781 attaccctcc cggtctatca ccgtgatgtc taaagctagg ttccccgaaa acacccctgg
     3841 atttggcgac gtctcggcag cgcttgccgt ctgattgggt tttgtgggaa gtaccacata
     3901 tacaattcct aaaatcaaag ccgccaaaaa gccgtacaga atccacttcc tagccattag
     3961 tgcttgaata atccccaaaa aaatccctcc gctctctata gcccaaacgc cggcagatag
     4021 cctgcaagcg actggagtac tccaaaatac atcaatatcc caagtacgat taataagtac
     4081 cctgaaatcc ggctaagcca aaccgtgatt ttggtaagtc ttgaccacct gccctgcagc
     4141 cgatcgatga gcaccgctgt cagaacaaat ggaagcccta accccacggc gtagatcacc
     4201 aggagaaaca tcccctgtgt cagggaagca gttgttgagg ccagaatcaa tatggatccg
     4261 aggataggcc caatgcacgg cgtccaaccg ctgctgaaag caagaccaaa gacaaaactc
     4321 tgcagaaaac ctgcatcttt cccttctttt gtcttcacct gttttctggt gtcgaaattg
     4381 agaaagccaa tcttaatcaa gcctgcaaga tgcagcccga atactataat gattacgcca
     4441 gaaatcttcc tgaggaggaa ctgatgcctc aaaaggaact gccccaagag tgtggccgag
     4501 gctcccagta ccacaaacac cacggtaaac cccagaacaa atcccaagga gttgcgcaaa
     4561 agcctgcccc ggttttcggc accgactcct gcaagatatg cgatgtaagc aggaataagg
     4621 ggcatgacac agggagaaaa aaacgagacc aaccctgcta aaaaagccag ccatattgag
     4681 atctgcgttg cttccatagc tatcctccct tgcgcacgac cttaacaaac accgcatata
     4741 ccccctcttc aagaggccaa agcagccgat ccggcacttt agcgctggtt tctttgtaaa
     4801 gaggccgccc ctgatagtag gcagacatag gtgtaagctt tctatctatt acagcaacta
     4861 ccgacaaagt cctttggtaa gttgttccct cgtgcaccag gtattcttcc acagcaataa
     4921 ctggaatggc atccccagtt ttctccaaac ggaagattcc gtacagcgaa ccggcaaaca
     4981 gtaccaggaa aactgccaga agccccaact cttttttgct catagatatg atcatcactt
     5041 ttcacctcca ttatacccca tgggggtatt tttgaaaaca gctagtttcc tccctccact
     5101 aaactgtatt cggtgcttcc gcattttctg cagatttccg cgaattctcc ttaccccata
     5161 aaaaaacgcc gcccttatcg ggcagcgtct cgaagaatac tatttcttga ttttagctgc
     5221 agcttcctca tccaagaaca gggttacgtc gcgatggagc tgcaggaggc ttgcaggaag
     5281 atccagtgtg actacgccgt ttgtggtgcc gtagatggca tcctgcttgc tctttccgct
     5341 tgcaagaaga atgatctctt tagatctcag gatggaacct acacccatgg ttacggcgcg
     5401 ggttggaacg tcttctttct tttcgaagaa cctggcgttg gcctcgattg tcgactgggc
     5461 aagggcaacc aggtggctgt ttgcctgcag gcagtttgcc ggctcgttga agccgatgtg
     5521 tccgttggtg ccgatgccga gaatcatcag gtcgattcca cctacatctg cgatagcctt
     5581 ttcgtaacgg aggcactctt cgttgggatc ttcagcaata ccgcttaaaa agtggatgtt
     5641 ttccttcttg atgttcacgt gggaaaacag attggtttcc atgaagtaac ggtagctctg
     5701 gggatgatcg ctatcaaggc cgaggtactc gtcaaggttg aaggttctaa ccttactgaa
     5761 gtccaaaccc tcttctttgt gcagccttac tagctccttg tacataccca ctggggttcc
     5821 acctgtggga aggcccaaaa caatatccgg cttagcttta atagcatcag ctacatactt
     5881 tgcagcttcg atgctcatct catcgtaagt actactaatt acgtatttca agtcgactct
     5941 ctcctttttc gtgtttccaa tccttgttat tctcctaagt aagacccact tcctgcaaat
     6001 tgtgcaaatt gtggcaccct cacacctgct tgtcagatct ggattgtcag gaaaactact
     6061 taaattccat accgcaccac ataggaaatt cataattatc ggagaacacc ttttcacaga
     6121 agggaggttc caccgatggt caaagttggc ttggaaattc ttctggaaaa tgaactgagc
     6181 acggtgtctg gcaaaagagt cggtctcatt acaaccccta caggcgtaga cagcaggctc
     6241 aactccaacg tggacctgct tttggagcac ggagtccgcg ttactgctct tttcggtccg
     6301 gagcacgggg taaggggtga tgctcaagca ggcgagtcgg tccaatcggg caaagatccg
     6361 aagaccggac tgcccgtcta ctccctctac ggtccgacca aaaagcctac caaagagatg
     6421 ctcgacgatg tagacgtttt gatttttgac atgcaggatg tgggctgccg ctactacacc
     6481 ttcctgtaca ctctggcgta tgctcaagaa gctgcagctg agatgggcaa ggagtttgtg
     6541 gttttggatc gcccgaaccc cctgggagga ctgactgtag aaggaaactt ggttaaggga
     6601 ggctttacct cgtttgtagg cggctattcc ctgcccatac gctacgggct tactatcggc
     6661 gagttcagcc tttatcttga aagataccac ggagtcggga agaagcccaa agtgatcatg
     6721 ctctccggtt ggaagcgggg aatgtacttt ggcgaaactg ccctgccttg ggtgctacct
     6781 tcgccgaaca ttcccaccgt tgacacggct ttcgtgtacc ccgggacttg cctttttgaa
     6841 gggaccaatc tctcagaagg caggggcact accaagccct ttgagatcat cggcgctccg
     6901 tggctcgacg gctttgccct tgcagatagg ctaaacagct tcgaacttcc cggcgtgatt
     6961 ttcagaccgg tgttcttcac cccaaccttc tctaaacacc agggaactgg ctgtcagggc
     7021 gtccagctgc acatcacaga ttacgacaac tttgcgccgg ttagaactgc cctcttcctc
     7081 ttatcagagg tctttaaata ccaggaatcc cgttttcttg aaacaaacgg ccactgcttc
     7141 ttcgatctta ttgcaggaga caagaaagtc agagagaatc tcaccgccaa agcaggaccc
     7201 caagagatct gggaagagat ggaaagcgaa aacaggtctt ttgtggaaaa agccaaatcc
     7261 atcctgcttt actaaacttg aggagatagc ttatggaaag tctagtttac gcagagccgg
     7321 catctttagg catcgatccg gaaaagttca aagaagccga agctgtgctt aaagaagaaa
     7381 ttgaaaaggg atctgctcca ggtgctgttg ccctggtgct ctaccaaaac aagatcgcct
     7441 accactatgc tttcggaaag agggccttag ttcccgaacc ggaagagata acccttgaca
     7501 ccatttacga ccttgcctcc atcaccaaat gcgtcgccac cacaactgcc tgcatgatgt
     7561 tgatggagca gggcttgata ggtttggaag accccatcgc caagttcttt ccagattttg
     7621 caggtaagga agagatcacc atcttccacc tgctcaccca cacttccggg cttcctgctt
     7681 ggctgccttt gtatcaggag gcaagaagca aagaggacta cagaaaagct ctggctgaaa
     7741 gcgatctgga acaccagcct ggggcaaaag tggtttacag ctgtttgggt ttcattctct
     7801 taggcctaat cctggaaaag gtcgcaggag aagagttgga cgcgtttttg caaaaagcgc
     7861 tctttgatcc tttgggtatg gaagacacgt gcttcaatcc caagaaaaat ttcggacccg
     7921 aaaaactcct gcgagttgct cccacagaag ctaccgaaga tagggggctt ctgcgaggag
     7981 aagtccacga cgaaaacgcc tactgcatcg gaggtgtgag cgggaacgcc ggtctcttct
     8041 ccacagccgt ggatctggca aagtttgctt ccatgctgct ttccggcggg gtgttccaag
     8101 gaagaaagat ccttgagcaa aaaaccattg agttgatgac ccaaaaccat accgctccag
     8161 ttacgcccaa tagaggcctc ggatggctga agaagaaccc gatctccagc ggcggcagcc
     8221 tcctgacaga tgcaagctac ggccacaccg gctttacagg aacatcaatg tggctcgatc
     8281 ccgtccttaa cgtggccatc atcctcctaa ccaaccaggt ccatccaagt agacaccagg
     8341 gcggagtggc caagcttaga tcgtcctttg ctgatgcagt tgtctcggcg ctgccaaaac
     8401 gctaaagaaa gcaaaagcac ccttgctgtt tctcaaaggt ctttaaggcc ttacgggaaa
     8461 cttggggtgc ttcttttttt tacttattca aaagctctgc aacctgcatt acgtctttgt
     8521 cccctcttcc ggacaggttg acgataatga tctgatcttt agaaagctgc ggagccaact
     8581 tcattgcatg ggcaacagcg tgcgagcttt caagagcggg aatgatgccc tcggttttgc
     8641 tgaggaattt gaatgcatcg agagcttcct ggtcggtaac tactgcatac tctgcccgac
     8701 cgatttcctt gtagtaggag tgttcgggac caactccggg atagtccagg cctgcggcga
     8761 tggagtacac cggcaggggt tcgcctgtct cgtcctccag ggagtagcat ttgaagccat
     8821 gaatctggcc ttttttccct ttagttaaag tcgctgcgtg gtagggtgtg tccagaccct
     8881 ttcctccggg ttctaccccg atgagcttta cctctttatc ttcaaagaac ggtgcaaaga
     8941 gtccgatggc gttgctccca ccgcctacgc aggcaacgag atagtcaggc agacggcctt
     9001 cgcgctctag gatctgctct ctggcttctc taccgatgac cgactggaag tctctaacaa
     9061 tcatgggata cggataagga cctaccgccg aaccgatcat gtagtacgtt gtctcgtagt
     9121 tggcaacaag gtccatgaac gcttcatcta cggcctcttt cagggttctt tcaccagact
     9181 taaccggaac cactttggcg cctagaagct ccatgcggaa gacgttaagc gcctggcgct
     9241 tggtatcttc ttcacccatg tagatgacgc actccatatc gaaaagagca caggctgtag
     9301 cggtagcaac accgtgttga ccggcacctg tctccgcgat aattcgcttt ttaccaagcc
     9361 tttttgccaa taggacctga ccgatggtgt tgttgatctt atgtgcacca gtgtggttca
     9421 ggtcttctct tttgaagtaa accttcgctc ctccgagctt ttcggagaac ttctctgcgt
     9481 agtagaggac gctgggtctg ccaacgtagt ctctaatgag atcctcaaac tccttctgga
     9541 attggggatc atccttgcac tcgaggtagc attgggcgat ctcatctagg accttctgca
     9601 cctcggcggg cacaaaagaa cctccgaatt caccgtaata gcccttttgg tcaggtaaca
     9661 tcatagtaga cataaaaaac ctccttgact agagtcaaag agccattagt aacaataagt
     9721 tcactctgct cagctaaact cagctcggct cgtcttgacc ctgtgtaatg aaacttaaag
     9781 ttaggtattc aaccgaattt gtaaatctcc tgcaattatt tggttaactt atgtttttta
     9841 tgaatagtat tcgtttatct tctcaaaata agtctaggga ggtgagacta tgcgccgcca
     9901 cttaccgttc tttctattgt tagcgctttt actcctaatt atttgggaca tcccctttgt
     9961 tccaaaggaa atttacgaac cccatctgga gagcttatat gctgccgacc atgaggaaga
    10021 aaacgcggtt cttcccgacg cccaaacagc ggacgtcaat cccgctgaca atctcatcgt
    10081 ctctccgttg catctggcaa gcttctgccc gtactgcgaa gcagaaccta ttatcaagcc
    10141 cggtgacagt tcagatgacc tgcctggcat tctggagttt ctcaatgata gaggctacta
    10201 caaaggcgag ctgacgtctc actacaccga tgaggcagtg gctgccgtta aaaccttcca
    10261 gctcgacaac aagctgaccg tcgacggcat cataggcgcc cagacctggc aggccatagg
    10321 cagctctttt gccactttac ctatggcaaa agccaagccc gagactgaca atctttctat
    10381 tttcgtcg
//
LOCUS       DAOSDA010000006         9949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_4462_length_9949_cov_3.731554, whole genome shotgun sequence.
ACCESSION   DAOSDA010000006 DAOSDA010000000
VERSION     DAOSDA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4462_length_9949_cov_3.731554"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PLW82_00345"
     CDS             <1..446
                     /locus_tag="PLW82_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOJ45760.1"
                     /translation="RRAFFDFDDTTVVTRLIEGNFPPYERVIPTSHTTKVKVDRRTIV
                     DAVDRIAVMVRGGSSLAVRLTVADNSLTINSGEMEYGSAKEQVYVEKEGEDLEIKVDY
                     RFLFEGLKAFTGESVEMMFKGPDSAILFKSKEMPGYIYLMMPLNF"
     gene            472..690
                     /gene="yaaA"
                     /locus_tag="PLW82_00350"
     CDS             472..690
                     /gene="yaaA"
                     /locus_tag="PLW82_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014423158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HOJ45761.1"
                     /translation="MEPVDVVIKTETIKLEQFLKWAGVVQTGGEAKLLIQDGYVYVNG
                     EEELRRGRQLKEGDLVEIEGSGKFKVAK"
     gene            705..1817
                     /gene="recF"
                     /locus_tag="PLW82_00355"
     CDS             705..1817
                     /gene="recF"
                     /locus_tag="PLW82_00355"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOJ45762.1"
                     /translation="MLLSKLSLEKFRNYKSASLTFGEGLNFLIGNNAQGKTNLLEAIF
                     YLCHLSSPRTSNDNDLILFGSEEAKVYGEVKTELGTYELEVVIPEKGRKKASVDGIVQ
                     SRYTDFQGHLTCVYFSPDDLKLVKGGPSERRRFMDLEISQVNPYYRHALNQYNKVLFQ
                     RNNLLKEGNFDPELLDVFSQQLVEAGSLIIEKRSSMLTRLNLLSKLLQRRLTELKESL
                     SLKYEPSFPIEGDVKNAFHKALKEAFPEEKNKGYTLIGPHRDDFSMYIDDIDVRTYGS
                     QGQQRTVVLALKLAELEFLKSESGEYPILLLDDVFSEIDDIRRRKLFEIVRQNRVQTF
                     ISTANPKDLEDIVGEQKRVFEIKRGCAGVKHDATFG"
     gene            1801..2049
                     /locus_tag="PLW82_00360"
     CDS             1801..2049
                     /locus_tag="PLW82_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOJ45763.1"
                     /translation="MLHLGENVVIPHSKVVAILDIESLGDSEANLEFLQTAKEEGFVR
                     NITKKAKSLVITTENIMYLSPISSVTLKSRFEKPYAEF"
     gene            2130..4067
                     /gene="gyrB"
                     /locus_tag="PLW82_00365"
     CDS             2130..4067
                     /gene="gyrB"
                     /locus_tag="PLW82_00365"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOJ45764.1"
                     /translation="MPENNKNSHYNEEQIRILKGLEPVRLRPGMYIGDTSMRGLHKLV
                     DEVIDNSIDEALAGFCDTIIVKLHKDGSVSVKDNGRGIPVGINKEVGKSTLEVILTHL
                     HAGGKFGGENSAYKYSGGLHGVGVSVVNALSEWLVAEVYLDGNIYQQKFARGIPQGDI
                     KIIGTTDDNGTYIAFKPDNTIFQNTTFDPKVLLERCRELAFLNKGLKIIFEDEIHDRR
                     EEFFYEGGIASFVSFLNTGKTPLHEDIIYFSAEKDEVIVEVAMQYTDEYSEISYSFVN
                     NINTSEGGTHLTGFRSALTKTINDYSRKVGLIKENEDNLTGDDVREGLTSVVSIKLQN
                     PQFEGQTKGRLGNAEVRGIVDTICTERLSIFFEENPSAARIIVEKALAALRAREAARK
                     ARELTRRKNALEVGTLPGKLADCSRKDSEGTEIYIVEGDSAGGTAKQSRNREFQAILP
                     LRGKILNVEKHGEDKILGNEEIKAMIRAFGTGYGDTFDISNSRYERIVIMTDADVDGA
                     HIRTLLLTFFYRYMRPLIEEGRVFAAQPPLYKIEERRDRADKWLKYAWSDEELEKYKA
                     EFEEAGKTYYIQRYKGLGEMDADQLRETTMDPKTRVMVRLTLEDAEEADRIFTTLMGE
                     EVEPRRAFIEEHALEVTDLDV"
     gene            4094..6523
                     /gene="gyrA"
                     /locus_tag="PLW82_00370"
     CDS             4094..6523
                     /gene="gyrA"
                     /locus_tag="PLW82_00370"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOJ45765.1"
                     /translation="MALEPSERIINIDINQEMKKSYINYAMSVIVDRALPDVRDGLKP
                     VHRRILYTMHQLGLGPRSQYKKSARIVGDTMGKYHPHGDSAIYDAMVRLAQDFSTRYP
                     LVDGHGNFGSVDGDPPAAMRYTEARLSEMALEMLRDIEKDTVDFIPNFDGEEMQPTVL
                     PSRIPNLLVNGASGIAVGMATNIPPHNLGEVIDGLVAIIDNPEITIQDLMNYVKGPDF
                     PTGGIILGRQGIRKAYLEGRGSIKVRAKAEIEEMRGGKFRIVISELPYTVNKANLIEK
                     IADLVRNKKLDGITDLRDESDKQIRVVIEVRKDVNPKILLNQLYKHTKMQETFGAIML
                     ALVDGQPKVLNLKEMMVEYLKHQEIVTTRRYKYDLEKAEHQAHIREGLLIALDNIDEV
                     IRIIRESPSDKEALDTLMATFGLTEIQGNAILDMRLRRLTSLESEKVMQEYKELQETI
                     AEFKSILGDRTKLMAIVKDDLLSIKSKYADKRRTHISAQSGTLDVEDLIAEEDVVVMI
                     TKQGYIKRLPLNTYRSQRRGGRGVIGMQTKDEDYVEHIFVANTLDTMLFFTTKGLVHQ
                     LKTYQIPDASRQSRGTSVVNLLQIDKEEIITAVISIKDFEEEADLMMITKQGRIKRTE
                     LKEYDTRLKRGLIAMKLEEDDELVSVVKVKDDDQVFIATSDGKAIRIEVPDVRCMGRA
                     TTGVKGIELSRRDFVVSMDVVKPGESYVLTITENGYGKRTRIDEYRTQGRGGKGLINM
                     KVNKKTGNVVGSRMVYGNEEEEVIIATREAIVLKTSVDRISVQGRSTQGVLVMRPEPG
                     DKVVAVALSEE"
     gene            complement(6577..7164)
                     /locus_tag="PLW82_00375"
     CDS             complement(6577..7164)
                     /locus_tag="PLW82_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45766.1"
                     /translation="MKRWVFVLLAVLVVSFSAFAAEKLLFEDDFEDELDLTMWFPLSD
                     EWQSGEGALVFPNVSFGDIFGGEEDWENYAVECEILPIEFGTYGSIRIFVRSNELWNG
                     YGVSFHTGGFMVHRFEGNWDIHTIFANETSPTFLAGVPFKARVEVYRTTLKVYINDEL
                     VWEGEDEEDWYFEGMFGFRADNSAVEIRNLKVYQL"
     gene            complement(7350..8747)
                     /locus_tag="PLW82_00380"
     CDS             complement(7350..8747)
                     /locus_tag="PLW82_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-glucosamine 2-epimerase"
                     /protein_id="HOJ45767.1"
                     /translation="MKALALITPVVLALSHATPAKAFQRGIQIDPGFAYYQGRTEESI
                     AEELKLNGFTIVHYFITNENNINVKLVEELKKAGLEVWALVLGNGSYSVANFPSNWES
                     WQMKHIKTSFPGFYHFCMNNEEYREWKKASLARVIQSAPFDGVELAESYLPEVNGFST
                     GGYACVCDTCLEIFREKWQDEAPEFVDQRDPRYYRRVPETYEKWIEFRVYTVSNFLND
                     IVNGKGGLREARPDIIVSTWSLGIDSGRRSVEMMRELQGLDDYDIVKTVRPDRHTIQT
                     HWPDWTKANLPPDYIKAYEPFAEPVREHFPEVELMLQTDIGSQEQMRRSDEWMEEFYK
                     VAEELGYHSVMSYEYHLGLGMYQNPPTLKMVTRLSGNRLQLSFDRRLDFFSSTDMSKF
                     TFLNHDSAYASMVVVDGNRIILLLGGVDDSEEFDLDIGEIESATDVLLLKGYPRNGKV
                     VGTIHVPPAGESYLL"
     gene            complement(9156..9695)
                     /locus_tag="PLW82_00385"
     CDS             complement(9156..9695)
                     /locus_tag="PLW82_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ45768.1"
                     /translation="MKIVNMTQLNETQIMQAAQILTDSIPIGWPTLQDALDEINELLV
                     PENTLLAAVENGDVIGWGGILPQYNGNVFELHPLAVRIDKRKQGTGRVIVTALEDEAR
                     KQGGLTIFVGADDEKDNGETSFANVDLFDDFPGKIKSFSSGTHQSGFYLKLGYKIIGV
                     MPDANGVGKPDIFLGKRLR"
BASE COUNT         2935 a         2268 c         2296 g         2450 t
ORIGIN      
        1 cgagaagagc gttcttcgat ttcgacgaca caacagttgt cacgaggctc attgaaggca
       61 acttcccacc gtatgaaaga gtcataccga cgagccacac aactaaagtt aaagtagata
      121 gaagaactat cgtagatgct gtagatagaa ttgcggttat ggttcgcggt ggaagctcgc
      181 ttgcagttag actaacagtt gcagacaact ctctcactat caactctgga gagatggaat
      241 atggtagcgc caaagaacag gtctacgtgg aaaaagaagg cgaagatctg gaaatcaaag
      301 tggactacag atttttgttc gaaggattaa aagctttcac cggcgaaagt gtagagatga
      361 tgtttaaagg tccagactcg gcaattctct ttaagtctaa agagatgccg gggtacattt
      421 acctcatgat gcctcttaac ttctagccaa aacttgaaag gtggcttttt tatggagcct
      481 gtagatgttg taattaaaac ggagaccatc aagttggagc agtttcttaa atgggccggt
      541 gtggtgcaga ccggtggaga agcaaagctc ctcattcaag acggctacgt ttatgttaac
      601 ggtgaagaag aactgagaag gggaagacaa ttaaaggaag gcgatcttgt agaaatcgaa
      661 ggaagtggta agttcaaggt cgcaaaataa gggagtggct tgtattgctg ctttcaaagc
      721 ttagtcttga aaaattccgc aactataaat cagcgtcgct tacatttgga gaaggactta
      781 actttctaat cggcaataac gcacagggta aaaccaatct tttagaagct attttttatt
      841 tgtgccatct ttcttcaccg cgaaccagca acgacaatga tctgattttg ttcggcagcg
      901 aagaggccaa ggtctacggc gaggtgaaaa ccgagctggg tacctacgag ctggaagttg
      961 tcattcccga aaaaggaaga aaaaaggcat ctgtagacgg catagtacag agccggtaca
     1021 cggattttca aggacactta acttgtgttt acttttcacc tgacgatctt aaactggtta
     1081 aaggcggtcc cagcgaaaga aggcggttta tggatctgga gattagccag gtgaatccat
     1141 actaccgcca tgccttgaac cagtataata aagtgctttt tcaaagaaac aacctgctta
     1201 aagaaggtaa ctttgatccg gagcttctgg acgtgttttc ccagcagctc gtggaagcgg
     1261 gatctttgat cattgaaaag cgcagttcaa tgcttacaag gctgaacctt ctctccaagc
     1321 tcttacagcg acgtcttaca gagctcaagg agagtctttc attaaaatac gaaccttctt
     1381 ttccaatcga aggagacgtt aaaaacgcgt tccataaagc tttaaaagaa gcatttccag
     1441 aagaaaagaa taaaggttat actttaatag gaccccaccg cgatgacttc agcatgtaca
     1501 tcgacgatat tgacgtcaga acttacggct cacaaggaca gcagcgcaca gtagtgctgg
     1561 cacttaagct tgcagagctg gagtttttga aaagcgagtc gggcgaatac cccattcttc
     1621 ttcttgacga tgttttttca gaaatagacg acataagaag aaggaaacta tttgagatag
     1681 tcagacaaaa tcgagttcaa acatttatct caacggcaaa tcccaaagac ttagaagata
     1741 tagtcggaga gcaaaaaaga gtttttgaga tcaaaagagg atgtgcgggg gtgaaacacg
     1801 atgctacatt tgggtgagaa cgtggttatt cctcacagca aagttgtggc aatattggac
     1861 attgaaagtc tcggcgactc tgaggccaac ttggagtttt tgcagactgc aaaggaagaa
     1921 ggttttgtaa gaaatataac aaaaaaggca aaaagtctcg tgataaccac agaaaacatt
     1981 atgtacttgt ccccgatatc atctgtgact ctcaagtcaa ggtttgaaaa gccctatgcc
     2041 gaattttaaa cagggaggtg aactaactgg ttgcgagtaa gtctcatcct tatagtttcc
     2101 atgctatgag cgataaacca gtagaaaata tgccagagaa caataaaaac tcccattaca
     2161 acgaggaaca aatacgaata ctcaagggat tagagcctgt tagacttcgc cccggtatgt
     2221 atattggcga tacatcgatg cgaggacttc acaagttagt tgatgaggtc attgacaaca
     2281 gtatcgatga agctttagca ggattttgcg ataccatcat tgtgaaattg cacaaagatg
     2341 gttctgtttc tgtaaaggac aatggcagag gtattcctgt cggcattaac aaagaagtcg
     2401 gcaagtctac tttggaggtc attctcacgc acctccatgc tggaggtaag tttggcggcg
     2461 aaaacagcgc ttacaaatac tccggaggtc tgcacggcgt aggtgtttcg gtagttaacg
     2521 ctctttcaga gtggcttgtt gctgaagtat acctagatgg caatatctat caacagaagt
     2581 ttgcccgcgg cattccgcag ggcgacatca agatcatcgg cacaacagac gacaatggta
     2641 cctacattgc tttcaaaccc gacaatacaa tcttccaaaa cacaactttt gatcccaagg
     2701 tgctccttga acgctgccga gagcttgcgt tcttaaacaa agggctaaag atcatctttg
     2761 aagatgagat tcacgataga agagaagagt tcttctacga aggcgggatc gcatctttcg
     2821 tcagcttctt aaataccggc aagacccccc ttcacgaaga cattatctac ttctcagctg
     2881 agaaagacga agtgatcgtt gaggtggcaa tgcagtacac cgacgaatac tcggagatca
     2941 gctattcatt cgtaaacaat atcaatacat ctgaaggcgg tacgcatctg acgggcttta
     3001 gaagcgccct tactaaaacc atcaacgact attcgcgaaa agtcggcttg attaaggaaa
     3061 acgaggacaa tcttaccggc gatgatgtca gagaaggact taccagtgtt gtctcgatca
     3121 agttgcagaa tccccagttt gaaggccaga ctaaaggccg cttgggtaac gccgaagttc
     3181 gcggcatcgt agacaccatc tgtactgaac gtctctcaat tttctttgaa gaaaacccgt
     3241 ctgctgcgag aatcatcgta gaaaaggcac ttgccgctct cagagcaaga gaagccgcaa
     3301 gaaaggctag agaactaaca agaaggaaaa acgctctgga agttggtact ctacctggaa
     3361 aacttgccga ctgttccaga aaagattccg aaggaactga gatctacatc gttgagggag
     3421 actccgctgg aggtaccgca aaacagagcc gcaaccgcga gttccaggct atcctacccc
     3481 ttaggggtaa gattctgaac gtggaaaaac acggcgaaga caaaatcctg ggcaacgaag
     3541 agattaaagc gatgattcgt gccttcggaa ccggctacgg tgacactttc gatatcagca
     3601 actcccgcta tgaacgcatc gtcatcatga cagatgctga cgttgacggt gctcacatca
     3661 gaacacttct tctgaccttc ttctaccgct atatgagacc gctcattgaa gaaggtagag
     3721 tttttgcagc tcagcctcct ctctacaaaa tcgaagagag aagagacaga gccgataaat
     3781 ggctgaagta tgcctggagc gatgaagagc tcgagaagta caaggcagag ttcgaagaag
     3841 caggcaagac ctattacatt cagcgctaca aaggtcttgg tgaaatggat gcggatcagc
     3901 taagagagac aaccatggat ccaaagacca gagtgatggt gcgccttacc ttagaggacg
     3961 ctgaagaagc agatcgcatc tttacaaccc ttatgggtga agaagtagaa ccgcgccgcg
     4021 cgtttatcga agaacacgct ctagaggtta ccgatcttga cgtttaacca gatgcagaaa
     4081 gaggtgataa accttggctt tagagccctc ggaaaggatt attaatattg acataaacca
     4141 agagatgaag aaatcgtaca tcaactacgc tatgagcgtt atagttgatc gtgcacttcc
     4201 cgacgtccgc gatggcttaa aacctgttca ccggcgcatt ctctacacca tgcaccagct
     4261 gggtttagga cctagatccc agtacaagaa gtcggcgaga atcgtcggag acaccatggg
     4321 taaataccac cctcacggtg actctgcgat ttacgatgcc atggtgagat tggctcagga
     4381 cttttccacc agatatcccc tggtagacgg ccacggtaac ttcggctctg ttgacggcga
     4441 tccgccagcg gctatgcgtt atacagaagc tcggctgtca gagatggctc tggaaatgct
     4501 gcgtgatatc gagaaagaca ccgttgactt tattcccaac ttcgacggtg aagagatgca
     4561 gcctacggtt ctgccatctc gcatacccaa tcttttagtt aacggagcat ctggtattgc
     4621 cgtcggtatg gcaaccaaca tcccacccca caacttaggt gaggttattg atggtttggt
     4681 tgcaattatc gacaaccccg agattactat ccaggacctg atgaactacg taaaaggtcc
     4741 agacttccca acaggcggta tcatcttggg acgtcaaggc ataagaaaag cctatctaga
     4801 aggcaggggc tccattaagg ttagagcgaa agctgaaatt gaagagatgc gcggcggtaa
     4861 attccgtatc gttatttcag aattacccta taccgtaaac aaagcaaacc tcatcgagaa
     4921 gatcgcagac ttggttcgca acaagaaact cgacggaatc accgatcttc gcgacgagtc
     4981 cgataagcaa attagggtcg ttattgaggt cagaaaagat gtcaatccaa agatcctact
     5041 aaatcaactt tacaaacaca ccaagatgca ggaaaccttc ggcgcgatca tgctggcact
     5101 agttgacgga caaccaaagg tactaaacct caaagagatg atggttgagt acctcaagca
     5161 tcaggaaatt gtcacgacca ggcgctacaa gtacgacttg gaaaaggcag aacaccaagc
     5221 tcacatcaga gaaggtttgc tcatcgcact ggataacatc gatgaagtta tcaggattat
     5281 tcgcgagtct ccttcagaca aagaagctct agatacgttg atggcaactt tcggcttgac
     5341 cgagatccag ggcaatgcca ttttggacat gcgcctcagg agactgacaa gccttgaaag
     5401 cgaaaaggtc atgcaggagt acaaagaact gcaggagacc attgccgaat tcaagagcat
     5461 tttaggcgac cgcaccaagc tgatggccat cgtcaaagat gacctcctca gcatcaaatc
     5521 caagtacgct gacaagagac gcacgcacat ttcggcacaa tcaggtacat tagatgtaga
     5581 agacctgatc gccgaagaag acgtagtggt catgatcacc aagcagggct acatcaagcg
     5641 tctgcctctt aacacctaca gaagtcaaag acggggagga cgcggggtca tcggaatgca
     5701 gaccaaagat gaagactatg tagaacacat ctttgtagct aatacgctgg ataccatgct
     5761 gtttttcacc accaaaggtc ttgtgcatca gctgaagacc taccagatcc ccgatgcgtc
     5821 gagacagtca aggggcacct ctgtggtcaa cctcttgcag atcgataaag aagagatcat
     5881 caccgcagtc atctccatta aggactttga agaagaagcg gatctgatga tgatcaccaa
     5941 gcagggtagg atcaaacgga ctgagctgaa agagtacgat acaagactca agcgaggcct
     6001 cattgccatg aaacttgaag aagacgacga gctggtctct gttgtcaagg tgaaagacga
     6061 cgatcaggtc tttatcgcaa cttccgacgg aaaagctatc aggatcgagg tgcccgacgt
     6121 caggtgcatg ggcagagcca ccaccggcgt aaaaggcatc gagctttccc gcagagactt
     6181 tgtggtttca atggacgtgg tcaagccggg cgaatcatac gttttgacca taaccgaaaa
     6241 tggttacggc aaacgtactc gcatcgatga gtaccggaca cagggcagag gtggcaaagg
     6301 cttaatcaac atgaaggtca acaaaaagac cggcaatgtt gtcggttcca gaatggtcta
     6361 cggcaatgaa gaagaagaag taatcattgc cactagagag gctatcgtcc tcaagaccag
     6421 tgttgacaga atatccgttc agggcagaag cactcaaggc gttcttgtga tgcgtcctga
     6481 accaggagac aaagtagtag cagtagcctt atcggaagag taaaaagacc aaaacaggca
     6541 taaaaaaagg gggtgctttc gcaccccttt cgttttttac agctgataga ctttcaagtt
     6601 gcggatttca acagcagagt tgtctgcgcg gaagccgaac ataccttcga agtaccaatc
     6661 ttcttcatct tcgccttccc agaccagttc gtcgttgatg taaactttca acgtggttct
     6721 gtaaacctca actcttgctt tgaagggaac acctgccaaa aacgtcggag aagtttcatt
     6781 ggcaaatatt gtgtggatat cccagttgcc ttcgaatctg tggaccataa aaccgcctgt
     6841 gtggaaggat acgccatagc cgttccagag ttcattggag cggacgaaaa tgcggataga
     6901 accgtaagtg ccgaactcaa tgggcagaat ctcgcactct acagcgtagt tctcccagtc
     6961 ttcttctccg ccgaaaatgt cgccaaagct tacattagga aacaccaagg cgccttcacc
     7021 ggactgccac tcatcagaca gagggaacca catggtaagg tccagttcgt cttcgaaatc
     7081 gtcctcaaaa agtaacttct ctgcggcaaa ggccgagaag ctgacaacta gtactgctaa
     7141 aagtacaaac acccaacgtt tcatgaaaaa ataaccccct ttagttattc cttccacata
     7201 gataatttta tctggataca gacaatctcc ttctgtaaaa ttactggaag cccagaaaat
     7261 tctggttttg gagaaaagag tggtttatag gggacacaaa aaagacaaga accacctaaa
     7321 acggtccttg tctttgtaat gttgttagtt tacagcagat agctttcccc tgcaggagga
     7381 acgtggatcg ttcccactac cttgccattt ctcgggtaac ccttcagaag cagtacatct
     7441 gtagcgcttt cgatttcgcc gatgtccaga tcaaactctt ccgaatcatc aacaccgcca
     7501 aggagaagaa tgattctgtt gccgtccaca acaaccatgg aagcataagc ggaatcgtga
     7561 ttaaggaaag tgaacttgct catatcagta gaactgaaga agtccaacct gcggtcgaag
     7621 gagagctgta gacgattgcc ggatagcctt gtaaccatct tcaaggtagg cgggttctgg
     7681 tacataccca atcctaggtg gtactcgtag ctcatgacgc tgtggtagcc tagctcttct
     7741 gcaactttgt agaactcttc catccactca tcgctgcgac gcatctgctc ctgagaaccg
     7801 atatcggtct gcaacatgag ttctacttca gggaagtgtt cccggacagg ttctgcgaaa
     7861 ggttcgtagg ctttaatgta gtccggcggg agattggctt tagtccagtc gggccagtgg
     7921 gtctggatgg tgtgcctgtc aggtctaaca gtcttgacga tgtcgtagtc atcaagacct
     7981 tggagttcac gcatcatttc aacgcttctt ctgccgctgt ctatgcccag actccacgtc
     8041 gaaacgataa tatccgggcg ggcttctctg agaccgcctt tgccgttgac gatatcgttc
     8101 aggaagtttg acactgtgta aactctaaac tcgatccact tctcataagt ttcaggaacc
     8161 cttctgtagt acctgggatc ccgttggtcc acaaactcag gagcctcgtc ctgccatttt
     8221 tcccggaaga tctctaagca ggtgtcgcag acgcaggcgt agccgccggt tgaaaatcca
     8281 ttaacctcgg gcagataaga ctctgccagt tccacgccgt cgaaaggcgc actctggatc
     8341 accctagcaa gagaagcttt cttccactct ctgtactctt cgttgttcat acagaagtgg
     8401 tagaatcccg ggaagctggt tttgatgtgc ttcatctgcc agctttccca gtttgaaggg
     8461 aagttagcca ccgaatatga gccgttgcca agaaccagtg cccaaacttc caaaccggct
     8521 tttttcagtt cctctaccag tttcacgttg atgttgtttt cattagttat gaagtagtgc
     8581 actatagtaa agccgttgag ctttagctct tcggcaatgc tttcctcggt tctaccctgg
     8641 tagtaggcaa aacctggatc gatctggatg ccccgctgaa aagcctttgc aggtgttgca
     8701 tgagacagag ccaaaacaac aggtgtaatc aatgctaaag ctttcatcaa gtatttactc
     8761 cacctcttaa acatcggatt ttgcaaagcc atgtcttcac ccctctagat ccttctttat
     8821 tcagctattc cccagactgg attgatttcc tccaaaaaca ccactgcacg ttgaaatgaa
     8881 gcatttatca gtcattttcg gcagacaaaa aagaaaggtt tccagacaac atcgcgtcag
     8941 gttaaacagc tgcgcgatca gttactcgtc taaaacctct catttttagg tgttttcggt
     9001 tttctatagt gtcttacttc ttaaaatacc atttgacgat gttgttctag atgacacaaa
     9061 aagcttgaag ttcacatctg aatgagttca gatgaaagta tgaatgaagc ggcaaaagcc
     9121 gccccattca tacatgctta tctcgctatt ttacattacc gtaagcgttt acctaaaaag
     9181 atatccggtt ttcctactcc gtttgcatca ggcattaccc cgataatctt atatcctagc
     9241 tttaggtaaa aaccggattg atgagtacca gaagagaagc ttttgatttt tcctggaaag
     9301 tcatcaaata aatcaacgtt tgcaaaggac gtctcaccat tgtctttttc atcatctgcc
     9361 cctacgaata ttgtcaaacc accctgtttt ctcgcttcat cttctaatgc tgttacaatt
     9421 accctgccag taccttgctt gcgtttgtca atgcgaacag ccagcggatg cagttcaaaa
     9481 acatttccat tgtactgtgg gagtatacca ccccaaccga tcacatctcc attttcaaca
     9541 gcagcgagca acgtgttttc cgggaccaga agttcattta tctcgtccag agcatcctga
     9601 agcgtcggcc agccaatcgg aatactatca gtcaagattt gtgcggcctg cattatctga
     9661 gtctcgttaa gttgagtcat gtttacaatc ttcatgtgga aaacctccag ttttttttag
     9721 ataagcatcg aatcaaatca acctggagga cttcgtgctc tagcatatac ttcagattaa
     9781 caaggatatc tgatacaaag catcatttca ccccgattca acacgtcttg ttgaattgat
     9841 tatattccgc taatagagtt ttttcaacaa attgatttgt tatcttttct accaacagtt
     9901 gagtccgtat aattgtgtaa aattggtttc cccatgaaat aaaagaagc
//
LOCUS       DAOSDA010000007         9481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_4742_length_9481_cov_3.202949, whole genome shotgun sequence.
ACCESSION   DAOSDA010000007 DAOSDA010000000
VERSION     DAOSDA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9481
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4742_length_9481_cov_3.202949"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PLW82_00390"
     CDS             complement(<1..493)
                     /locus_tag="PLW82_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ45769.1"
                     /translation="MEGKVAVVTGGSRGIGLEIAEVFLQSGAKVAVLASSADSVAKAL
                     AHLQERYASPVMGVACNVADLEEVTAAMGKVEAELGPIQILVNNAGITRDALFLRMGE
                     DKWDEVISVNLKGVFNCTKAVFRSMVKNRYGRIVNVTSVVGITGNVGQANYAAAKAGV
                     IGFT"
     gene            complement(499..1404)
                     /gene="fabD"
                     /locus_tag="PLW82_00395"
     CDS             complement(499..1404)
                     /gene="fabD"
                     /locus_tag="PLW82_00395"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOJ45770.1"
                     /translation="MSEEKTAVVFPGQGAQYVGMGSSFFAADSEAREVLAQCSEGSGM
                     DLEKLILEGPEELLAQTAVTQPAIFAVSMAIYRVLKSRTGLVPDFAAGFSLGQYSALA
                     AAGAFDIKKGAALLKHRGSFMQDANGGAMAAVIGMEPESIKEVLNQVDGYVDIANYNS
                     PGQTVISGTESAVGEAVEALLKKGAKKAVPLKVSGAFHSKLMEGAASRFKEVLAGETF
                     GKTQFPVIGNETAREIEDVGFSLYTQLVSPVRWQDSIEYLRERGVTRIIEVGPGKVLS
                     GLIRRIDRSLEVVSVEEIDDLEKLR"
     gene            complement(1416..2399)
                     /locus_tag="PLW82_00400"
     CDS             complement(1416..2399)
                     /locus_tag="PLW82_00400"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOJ45771.1"
                     /translation="MNNTSQIGVLGLGAYVPERVLTNHDLEKMIDTSDEWITTRTGIK
                     ERRIASEGEASSDLGAKAALKALEDAKVDPAEIDAIVVATLSPDHIFPATACIIQEKI
                     GAVKAAAFDLSAGCSGFVYALAVAYGLVQTGLEKVLVIGAETISRFVNWQDRSTCVLF
                     GDGAGAVVLGRVERGGILGFDLGADGSGHSILNIPVGGSREPITPENLAQNRHYLYMV
                     GSEVFRFAVRTVPRTSRKALKKAGLEVGDVDWLIPHQANTRIIDAAVNLMGIESEKVV
                     VNLDKYGNTSAASIPIAWAEAAEDGRIKRGDKLLLVGFGAGLTWASMVLEY"
     gene            complement(2403..3386)
                     /gene="plsX"
                     /locus_tag="PLW82_00405"
     CDS             complement(2403..3386)
                     /gene="plsX"
                     /locus_tag="PLW82_00405"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOJ45772.1"
                     /translation="MKIALDAMGGDYAPEATVEGAVLATQLWGLETVLVGDTERIKPL
                     LEKAGTEMDIVHADETIGFDEDPAKAVRKKKNASIVVAAKLVASGGADALVAAGSTGA
                     AMAASLLIYGRQKGIGRPAIAFPMPTVQGMCLVLDGGANPGATAENLRDYGLIGSIYM
                     EKVMGVDRPKVGLLNIGEEEIKGTPEVVEAYQLLKNEKINFIGNIEGKDITAGAADVV
                     VCDGFVGNIVLKYTEGLANGLMSLIKDAMTANLKGKIGGLLVKGNLKSLKKKLDYEEY
                     GGTPLLGLKKLTIISHGRSSARAIANAIRVASEASSKNIIESIDEALEKRV"
     gene            complement(3383..3961)
                     /gene="fapR"
                     /locus_tag="PLW82_00410"
     CDS             complement(3383..3961)
                     /gene="fapR"
                     /locus_tag="PLW82_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HOJ45773.1"
                     /translation="MSRLPKKERQSLLLEKIKEDPFLTDEILAEKFKVSIQTIRLDRL
                     ELGIPEVRQRTKNLAQKAYSQVKSLKSKEIIGELIDLNLGKDGISMLEATEEMALSNT
                     GVVRGHFIFAQANSLAVSLVDSEVALTGSATVRYHRPVRVGERLIAKAKVREIKKNRY
                     LIDVETRIEDEKVFTGEFLAFALSGEGQEVES"
     gene            complement(4235..4408)
                     /gene="rpmF"
                     /locus_tag="PLW82_00415"
     CDS             complement(4235..4408)
                     /gene="rpmF"
                     /locus_tag="PLW82_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOJ45774.1"
                     /translation="MAVPKRRVSTTRKAKRRTNWKLRTPSINVCPNCGEPRLPHRACT
                     ACGTYKKPAAAAK"
     gene            complement(4427..4966)
                     /locus_tag="PLW82_00420"
     CDS             complement(4427..4966)
                     /locus_tag="PLW82_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOJ45775.1"
                     /translation="MRDLIRDLRISVANLDSGDDRSFEFFLPQDAWQIPDDQVEPRAP
                     LEIEGNIVHAGDFFVLKVNVKTRLKMECTRCLSDFEQDFVLDIEEELKKADSYDTLSD
                     ELEDEEESYSTFSGQEINLGEIIVDHMVLGLPFQRLCDENCKGLCPHCGQDLNKGRCD
                     CEEEKIDPRLQALKNLLNE"
     gene            5367..5861
                     /locus_tag="PLW82_00425"
     CDS             5367..5861
                     /locus_tag="PLW82_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45776.1"
                     /translation="MAQEDKEWVQIVSERTKERVLDVMIQRAVLTGSTRIQVTYDDLS
                     KLSGLSKGAIFKAVKALEAENKIKKLPSRARRIPNTYEIYTNVEIPEPKASEIVDLPE
                     YLEMTRSLKAKLRRLEQENYELKQILFPDAKKAPDIVSKSQITDDTVMLVFKIPKNSE
                     FEGF"
     gene            complement(5943..6161)
                     /locus_tag="PLW82_00430"
     CDS             complement(5943..6161)
                     /locus_tag="PLW82_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45777.1"
                     /translation="MVYKDSDELKKILERFAGSGWDLIEAPAKLYLEGSGDKEKLVSA
                     IRQADQECGSCGCEYDAMYKRALELLQG"
     gene            complement(6200..6832)
                     /locus_tag="PLW82_00435"
     CDS             complement(6200..6832)
                     /locus_tag="PLW82_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOJ45778.1"
                     /translation="MKEAVYTSIKSDATIEFIEKKSRFIGHARPVQDVEEAERFIDSI
                     KNLHPSATHNVWAYRLGISGQIQKFSDDGEPSQTAGMPTLDVILKQDITQVVIVTTRY
                     FGGILLGAGGLVRAYSKAAADAIAAAGVVSYGRREQYRFSVAYSEWGALQNHCEKRSW
                     TLKDIDYAEHVTCTAVIKEGEAEDLSAFLQDLTSGRVSPQFVSSIYLPID"
     gene            6999..8177
                     /locus_tag="PLW82_00440"
     CDS             6999..8177
                     /locus_tag="PLW82_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOJ45779.1"
                     /translation="MKDWNLSLLANLIRKNPKISRAELTALTNLSPTTVSSLVAELID
                     DGLVRETGVRDSQSGRKPILLEFDPEGRIGIGVSINHDGIKVALVNLLGEPLYIFEGR
                     ASLEHEKAVLAQVEQGIEECIAHAKEQNLQDRIYGIGIGVLGLVEGTKSRYLLGNTWH
                     SIDLAPIVEKHPKFYFSIENDINAMALGEVWFGQGKEAKHLLYVYLGYGLGVGVIIDG
                     KLYRGAFGSAGELGHVSVSHGDFKCRCGLTGCLGVIADRAHVMLRIKRAIEEGTSTSI
                     SPDNLTFESFLDAAKTGDPFCNELLDELISNLSRAIISAVNLFDPGLVIIGGFFSDPK
                     HPASSRLISTIQNNSLASYRDDFRILASNFGHNSGVIGAAAAVLKNVFDLQLTQEQIS
                     "
     gene            complement(8200..8751)
                     /gene="lepB"
                     /locus_tag="PLW82_00445"
     CDS             complement(8200..8751)
                     /gene="lepB"
                     /locus_tag="PLW82_00445"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOJ45780.1"
                     /translation="MLKHIRDWLITILEAAALTIFLLVFVAQSFLVEGYSMEPTLENG
                     ERVMVEKVSYRFRDPKRGEIIIIKNPLKRRDIYIKRVVGLPGDTIEVKNGQIFINGIL
                     LEEDYVLEPFNSWYSGTFFVPEGHVFVLGDNRNHSDDSRRIGPIPYGDVIGRVMFVYW
                     PFDKFEFLEPPKALANLDQSFDN"
     gene            8795..>9481
                     /locus_tag="PLW82_00450"
     CDS             8795..>9481
                     /locus_tag="PLW82_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45781.1"
                     /translation="MRPLQLPSYVQEIVNVLNQAGHEAFVVGGAVRDYLLGEIPHDYD
                     IATSATPKEVIALFPRTTSYGMKFGSVTVLAKRPVEVTTFRREGRYMDARRPLDVSFS
                     KKIDDDLLRRDFTINAIAYSPKTGIYDPLCGIGDLELKIIRAVGDPVERFREDALRIL
                     RAVRFLAKLGPPWQIEEKTLEAMAASSHLVKKLSKERITDEIVRIASSRFASYGLEAL
                     EKILETAFGHP"
BASE COUNT         2317 a         2550 c         2172 g         2442 t
ORIGIN      
        1 tagtaaagcc gatgacgccc gcttttgccg cggcgtagtt ggcctgaccc acgttgccgg
       61 tgatacctac aactgaggtt acattgacaa tgcgtccgta gcggttttta accatcgatc
      121 ggaaaaccgc tttggtgcag ttgaaaaccc ctttcaggtt gacgctgatc acctcatccc
      181 acttgtcctc acccattctg agaaacagcg catcccttgt gataccagcg ttgttaacga
      241 gaatctgaat cggaccgagc tctgcctcga ctttacccat ggcagcggta acctcttcca
      301 gatcagccac gttgcatgct acacccatca caggagaggc gtatctttcc tgcaggtgcg
      361 ccaaggcctt tgctacagaa tcggcacttg acgccaaaac cgcaactttt gcaccgcttt
      421 gcaggaaaac ctctgcaatc tccaatccta ttcccctaga accgccggtg acaacggcta
      481 ctttcccctc catctgtctc acctcagctt ttccaagtca tctatctctt caacggacac
      541 gacctcaagg gatctgtcga ttctacgaat cagaccgctg aggaccttgc cggggccgac
      601 ttcaataatt ctcgtaacgc ctctctcgcg caggtactca atggaatcct gccagcgaac
      661 cggcgatacc agctgagtat ataagctaaa acctacatct tcgatctctc tggcagtctc
      721 gttgccgatg actgggaact gggttttgcc aaaggtttct ccggccaaaa cctccttgaa
      781 cctggaagcg gcaccctcca tgagtttgga gtgaaaagct cccgatacct tgaggggtac
      841 tgcttttttt gcgccctttt tgagcagagc ttctaccgct tctcctacgg cgctttcggt
      901 cccagaaatc accgtttgtc ccggagagtt gtagtttgca atatctacat acccatcaac
      961 ttggttcaaa acctctttaa ttgactctgg ctccatgccg ataactgcgg ccatagctcc
     1021 gccatttgcg tcctgcatga agctgcctct gtgcttcagc agcgcggcgc cttttttaat
     1081 gtcaaacgca ccagctgcag caagagcaga gtactgcccc aagctgaagc ctgcagcaaa
     1141 atcgggaact aggccggtgc ggctttttaa cacccgatag atagccatcg atacggcaaa
     1201 aatcgcaggt tgggtaactg cagtctgggc cagaagttct tccggacctt cgagtatgag
     1261 tttttccaga tccattcccg acccttcaga gcactgggct agtacctccc tcgcctcgct
     1321 gtccgctgcg aagaacgagc tgcccatacc tacgtactgg gctccctggc cgggaaacac
     1381 tacagcagtt ttttcttccg acatcgtccg acctcctagt actcgagaac catagaagcc
     1441 caggtgagac ctgcgccaaa gcccaccaga agcagtttat cgcctctttt gattctccca
     1501 tcttctgcgg cttctgccca ggcgatggga atcgaagctg cagacgtatt gccgtattta
     1561 tccagattca ccactacctt ttcactttct atccccatga ggttcacagc tgcgtcgatg
     1621 attctggtat tggcctgatg gggaatgagc caatctacat ctccaacttc caagccggct
     1681 tttttcagcg cttttctgct ggttcggggc acggtcctta cggcaaagcg aaagacctct
     1741 gagccgacca tgtagaggta gtggcggttt tgcgcaaggt tttctggagt tatcggctct
     1801 ctagaacctc ctacaggaat gttcagtatg ctgtgcccgc tcccgtcggc tcccagatcg
     1861 aagcctaaaa tcccgcctct ttctactctg cccagaacca ccgcacctgc accgtcgcca
     1921 aacagcacgc atgtggagcg gtcctgccag ttgacgaagc ggctgatggt ctcggctccg
     1981 attaccagga ctttttccaa gccagtctgg accaagccgt aagctactgc aagagcatag
     2041 acaaagccgg aacagccggc actgaggtca aaagcagcag ctttcaccgc tccaatcttc
     2101 tcctggatga tacaagcggt agcgggaaag atgtgatccg ggcttaaagt cgcaacaact
     2161 atggcatcga tctctgcagg atcgactttg gcatcctcta gagctttcag cgctgcttta
     2221 gctcctagat cggaggaagc ttctccctca gaggcaatgc gccgctcttt gatgccggtc
     2281 ctagttgtaa tccattcatc agatgtatct atcattttct ccaaatcgtg gttggttaag
     2341 accctctcag ggacataggc tcccagtccc aggacaccta tctgcgaggt attgttcatg
     2401 aactaaaccc tcttttctag tgcctcatca atgctctcaa tgatattttt actggatgct
     2461 tcacttgcta ctctaatggc gttggcaatg gccctagcac tggatcttcc atggctgatg
     2521 atggttaact tcttgagacc gagaagagga gttcctccgt attcttcgta gtccagtttc
     2581 tttttcaaac ttttgaggtt ccctttgaca aggagccctc caattttgcc tttgaggttt
     2641 gcggtcatcg catctttaat cagagacatc aatccgttgg ccagtccctc ggtatatttc
     2701 aggacgatgt tgcccacaaa accgtcgcag accaccacat cagcggctcc tgcggtgata
     2761 tctttgcctt cgatgttgcc gatgaagttg atcttctcgt ttttcaatag ctgataggct
     2821 tcgactacct ccggtgtgcc cttaatctct tcttcaccga tgttcaaaag cccgactttc
     2881 ggccggtcca cgcccatgac tttttccata tagatgctgc cgatgagccc gtagtcccgc
     2941 aggttttctg ctgtcgctcc cggatttgca cctccatcta gaaccagaca catgccctga
     3001 actgtgggca tgggaaaggc gattgcaggt ctgccgatac ctttctggcg gccgtagatg
     3061 agcaggcttg cagccatggc tgctccggtg cttcctgctg ctacaagtgc atcagcaccg
     3121 ccacttgcaa caagctttgc agccaccact atagatgcgt ttttcttctt gcgcacagct
     3181 ttcgcaggat cttcatcgaa gccgatcgtt tcatcggcat gaacgatatc catctccgta
     3241 cctgctttct ccagtagggg cttgattctc tcagtatcgc ctacgagaac cgtttcgaga
     3301 ccccaaagct gtgttgccaa cactgcccct tctacagttg cctccggggc atagtctccg
     3361 cccatggcat cgagagctat tttcatgatt ctacctcctg tccttcacca gacagtgcaa
     3421 atgccaaaaa ctcacctgta aagacctttt catcctctat tctagtctca acatctatga
     3481 gataacgatt ctttttgatc tcacggacct tcgctttggc aatcagccgt tcaccaaccc
     3541 tcaccggtct gtggtaccgc acggtggcag aacctgtgag ggcaacttcg ctatccacaa
     3601 gggaaactgc caaggagttg gcttgcgcaa aaataaaatg acctctcaca acgccggtat
     3661 tgcttagggc catctcttcc gtagcctcga gcatgctaat accgtctttg cccaggttca
     3721 ggtcgataag ttccccaata atctccttgc tctttagaga tttcacctgc gaataggctt
     3781 tttgagccaa gttcttcgtc ctttgcctta cttcaggtat gcccagctcc agtcggtcca
     3841 gccttatggt ttggattgat accttgaatt tttctgcaag aatctcatct gttagaaaag
     3901 gatcctcttt gattttttcc aacagaagag attgccgctc tttcttcggc agccgcgaca
     3961 tggacgctcc ctccttttta gtgacctcgt actaatatct agtgttatta taaccgatag
     4021 atgccgtcaa gacaagaact tcatgccgga aggtacaagc tgtcaagtgg tggcattccc
     4081 ctttaggttt ggcgggggca aaaccatttc ctgcttgcaa gaagaccgag aaaggttctc
     4141 ccagtctgaa agcacgcttg tctcggtctc gtgaggccag gtcacagagg tttaaaataa
     4201 aaaaaccgag cgacaagctc ggtacctacc tccgttattt tgctgcggca gcaggctttt
     4261 tatatgtccc acaagctgtg caggcgcggt gcggcaacct gggttcacca cagttcggac
     4321 aaacgttaat tgatggagtt ctaagcttcc agttcgtacg tctttttgcc ttacgtgtag
     4381 tcgaaactct cctctttggt actgccatgt tctacacctc ctagggctac tcgttaagta
     4441 agtttttcaa agcctgcaat ctaggatcaa tcttttcctc ttcgcaatcg cagcgccctt
     4501 tgtttaagtc ctgaccgcaa tgaggacaga gtcctttgca gttttcgtcg catagtcttt
     4561 gaaacggcaa gcccaaaacc atgtgatcga cgatgatctc gccgagatta atctcctgac
     4621 ctgaaaacgt cgaatacgac tcttcttcgt cttcgagttc atctgacaga gtatcataag
     4681 aatcggcttt tttcaactct tcttcaatat ccaaaacaaa atcctgctcg aagtcgctca
     4741 aacaacgggt gcattccatt ttgagtctgg tcttcacatt taccttcaaa acaaaaaaat
     4801 cacctgcgtg cacaatgttt ccttctatct caaggggagc acgaggctca acttgatcat
     4861 caggtatttg ccacgcatct tgaggcagga aaaactcgaa tgacctgtca tcaccagaat
     4921 ctagattagc aacgctaatc ctaagatctc gaatcagatc ccgcatacta tctggcacct
     4981 cactaataat gctgcagaaa aaacacttgt acttgttcga agcaatggtc aatagagagt
     5041 tcagactgtt tcgggcagtt acctacttat tatagccaac tgctacttag tcgtcaagga
     5101 aaattacttg gcaacgaatc tgtgctcacc cttatcatta tacacagcgt ctcaatctgc
     5161 tgcaagcatc ttccgggcaa gtggaggtct aaggctgcac ttaatatagg caaaatcgcc
     5221 tcagtttact ccaactcata tatcatacaa tgctataacc ccaaaaggct gatattaaac
     5281 tgaattaacc gttaatattt tccgttaact agcatattat atattactcc gattaaacac
     5341 accggaatat gaaaggaaga ataagtatgg ctcaagagga caaagagtgg gttcagattg
     5401 tctcggaaag aaccaaggag cgggttttgg atgttatgat tcagcgggcg gtgcttaccg
     5461 ggagcaccag aatccaagtt acctacgacg acctgtcgaa gctttccggc ctttccaaag
     5521 gggccatttt caaggcggtc aaagctctag aagcggaaaa taagattaag aagctgccgt
     5581 ctagagctcg gaggattccc aacacctacg aaatctacac caacgttgaa atccccgaac
     5641 ctaaagcatc cgaaatagta gacttgcccg agtacttaga aatgaccaga tcgctgaaag
     5701 cgaaactgcg caggctggaa caggagaact acgaactgaa gcagattctc ttccccgatg
     5761 ctaaaaaagc cccagatatt gtctccaaat cgcagattac agacgatacg gttatgttgg
     5821 ttttcaagat tcctaaaaac agcgaatttg aaggatttta atgttactct cttcctttaa
     5881 aaactcgcct tccggcgagt tttttcatat ggttctttct gccactacaa cattttgcag
     5941 cgctagcctt gtaaaagctc gagcgctctt ttatacatag cgtcgtactc gcacccgcag
     6001 cttccgcact cctggtctgc ttgcctgatg gcagagacca gtttctcctt gtcaccgctt
     6061 ccttcaaggt agagtttcgc gggagcttcg atcaaatccc aaccggaacc ggcaaagcgc
     6121 tctagtatct tcttcaactc gtcggagtct ttgtacacca tctctcacct cacaagtccg
     6181 aacttatatg caaggacagc taatctatgg gcagataaat gctagagaca aactgcggcg
     6241 acactctacc tgaagtaagg tcctgtagga aggctgaaag gtcttctgct tctccctctt
     6301 tgatcactgc ggtgcaggtc acgtgctctg catagtcgat gtcttttaga gtccagctgc
     6361 gcttttcgca gtggttctgc agggcacccc actcgctgta ggccacgctg aaccggtact
     6421 gctctctcct cccgtagctc acgactcccg cagcagcgat ggcatcagca gccgctttac
     6481 tgtaggctct gactaaaccg ccggcaccga gtagaatccc cccaaagtac ctagtagtta
     6541 caattacaac ttgggtgatg tcctgcttta aaatcacatc cagcgtgggc attcccgccg
     6601 tttgagacgg ctcgccatcg tcggaaaact tctggatctg gccggatatg cccagcctgt
     6661 acgcccaaac gttgtgggtg gcactaggat ggaggttctt gatggagtcg ataaaccgct
     6721 ctgcttcctc cacgtcctgt acgggacgag cgtggccgat aaagcgggac tttttctcta
     6781 taaactcgat tgttgcgtcg cttttgatac tggtataaac agcttctttc aatgccttca
     6841 ctccacagta aattgttttt ataccaagct ttaccaaagg tagggcaaat aggcccaaaa
     6901 ggaatttccc ggaatttata gaaatcacaa agaaaagccg ttagtccctt atgaaagctg
     6961 gtgatgtcgt gcaatacaga actggtagtc agcaacttat gaaggattgg aatctatccc
     7021 ttttagcaaa cctcatcaga aaaaacccga agatctcgcg ggcagaactc actgcgctta
     7081 ctaatctatc tccaacaacg gtctccagct tagttgcaga gttgattgac gatggattag
     7141 taagggagac tggggtgcgc gactctcagt caggaagaaa gcccattctc ttggagtttg
     7201 atcctgaagg ccgcatcgga attggcgtct ccatcaacca tgacggcatc aaagtggcat
     7261 tggtcaatct gttgggagag cccctttaca tcttcgaagg tagagcaagt ctggagcacg
     7321 aaaaagcagt cctcgctcag gtggaacagg gaattgaaga gtgcattgcc catgccaaag
     7381 agcaaaacct ccaagaccgc atttacggta tcggcatagg ggtcttgggc cttgtggaag
     7441 gcacaaaatc cagatatctc ctgggcaaca cctggcacag catcgatctt gcaccaatag
     7501 tggagaagca ccccaagttc tacttcagca ttgaaaatga catcaacgct atggccttgg
     7561 gggaagtctg gtttggccag ggaaaggaag caaagcatct tttgtacgtt tacctcggct
     7621 acggtctggg tgtaggcgta atcatcgatg gaaagctcta ccgcggcgca tttggtagcg
     7681 ccggagaact cggacacgtc agtgtttccc acggagactt caagtgtcgc tgcggcctca
     7741 ctggctgctt gggggttatc gctgacagag ctcacgtgat gctgagaatc aagagggcta
     7801 tcgaagaggg aacgtccaca tcgatctctc cagataacct cacctttgag agcttcctgg
     7861 acgctgcaaa aactggggat ccgttctgca acgagctgtt ggacgagctg atctccaacc
     7921 tctcgagggc aattatctcg gcagtgaacc ttttcgatcc gggtttggtc atcatcggcg
     7981 gcttcttctc cgatccgaag catccggcat ccagcaggct tatcagcact atccagaaca
     8041 actccctggc cagctacagg gatgacttca ggattcttgc atcaaacttt ggccacaact
     8101 ccggcgtcat cggcgcagct gctgcggtgt tgaaaaacgt gttcgatctg cagctgacac
     8161 aagagcagat cagctaagat aaagaggggg tacccctctt taattatcga aactttggtc
     8221 gagatttgct aaagccttcg gcggctctaa aaactcgaac ttgtcaaagg gccagtagac
     8281 aaacataact ctgccgatga catccccata gggaatcgga ccgatcctgc ggctgtcatc
     8341 gctgtgattg cggttgtctc ctaagacaaa cacgtgacct tcgggaacga agaaggtgcc
     8401 cgagtaccat gagttgaaag gctctagaac gtaatcctct tccagaagga tgccgttgat
     8461 aaaaatctgg ccgtttttca cctcaatggt gtcgccggga agccccacta ccctcttaat
     8521 gtagatatcc ctgcgcttca gcggattttt aatgatgatt atctctcccc gttttgggtc
     8581 gcgaaagcgg tagctgacct tctccaccat taccctttct ccgttctcca gcgtcggttc
     8641 catagagtag ccttcaacta agaacgactg ggcaacgaag actaagagaa atatggtaag
     8701 agctgcagct tcaaggatag ttatcagcca atctcgtata tgcttaagca taggaacaac
     8761 ctcctaggct gaagaaaatt gcgtaaatca ggcggtgaga cctttgcaac tgccttcgta
     8821 tgtacaggaa atagtaaatg ttttgaacca ggcgggacac gaagcctttg tagttggcgg
     8881 cgctgtgaga gattaccttc ttggggagat cccccatgat tatgatatcg caaccagcgc
     8941 tactccgaaa gaagtaatcg ctttgtttcc aagaactaca tcctatggca tgaagttcgg
     9001 ttcggtaacg gtccttgcca agcggccggt agaagtgacc accttccgca gggaagggag
     9061 gtacatggat gctaggcgcc ctttggatgt cagcttctca aaaaagatcg acgacgacct
     9121 attaaggcgg gatttcacca tcaatgccat cgcctattcc ccgaagaccg ggatctacga
     9181 tcctctatgc gggataggcg atttagagtt gaagatcatc cgcgctgtag gcgacccagt
     9241 agagcggttt cgcgaagatg ccctgcgaat cctcagggct gtaaggttct tagccaaact
     9301 gggccctccc tggcagatcg aggagaaaac cttagaggct atggcagctt cttcccactt
     9361 ggtaaaaaag ctaagcaagg agcggatcac cgatgagatc gtaaggatcg cttcaagtcg
     9421 attcgcatcc tacggattgg aagccctgga gaagatctta gagaccgcat ttgggcaccc
     9481 c
//
LOCUS       DAOSDA010000008         8952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_5103_length_8952_cov_3.303023, whole genome shotgun sequence.
ACCESSION   DAOSDA010000008 DAOSDA010000000
VERSION     DAOSDA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5103_length_8952_cov_3.303023"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLW82_00455"
     CDS             <1..229
                     /locus_tag="PLW82_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOJ45782.1"
                     /translation="NQIGQGKYLIDIQREDVLPGDLIFFEGYGHVGLAISHYEFIHAT
                     TYSKPVVQVSEIDCPIWRDRIIQYSRYRLEK"
     gene            complement(248..1264)
                     /locus_tag="PLW82_00460"
     CDS             complement(248..1264)
                     /locus_tag="PLW82_00460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ45783.1"
                     /translation="MKVLLLNHYFRCGQSVHVEMLAKALARRGFDVSIYFNNNFRYLR
                     SRTAIIEAFRLQLNALAEAGVNVLGSLPKKVSQDTIIHAHSALTWRKGLELAKASGCP
                     FAVTIHGTGLERYTDVMQQADLVLSVAEVFAAPYRSFAENIIVLGNAVELDVHKPQKK
                     HTTKVMLTYLGRYADSKRVGLIALNNAIKTLRKDRPVEARVIGNIPRGIFDEVNYIKQ
                     LGWQTDRAKWLGDSDIVIGTGMAIREGMAAANVGYVMGKFIDGIVQPDLLPPVPEFTG
                     RESKVWPSPRTIVSDLAPLIENREALRLLQYKSYEFAKKYFDFEYFLDRIIDYYQGAF
                     EKKK"
     gene            complement(1428..2813)
                     /gene="pepV"
                     /locus_tag="PLW82_00465"
     CDS             complement(1428..2813)
                     /gene="pepV"
                     /locus_tag="PLW82_00465"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HOJ45784.1"
                     /translation="MLNKLQGEIKKLMPEMIESISEMIKIPSVEDPKTQSPEKPQGEG
                     CYRALMKMEEISRELGFSFKNVGNVAGHAEWGSGDEIVAVLGHLDVVPAGTGWDDDPF
                     SGKVEDGYIWGRGALDDKGPTIACLYAQKALMNLGFKPKKRIRTIVGTAEETNWKCMT
                     AYFNSEEIPTYGFTPDGQFPIIQSEKGILHLEIHGPLTDKFRAFAGERANVVPNRAFA
                     KVPAGLKVEAPLEEVKEADLAEPEALLGPSPEGNFKFVWAKGLEAHGSTPQLGHNAII
                     DLFIGLDKMGVQHPMVQFFAKVGHSPDGAALDIDFEHEIAGKLSMNPGVLRVTQDQCK
                     VTIDIRYPYGVTIEEIVERVKRHLVPGLEVKPLSVGGDTTKAPLLVENDHPLIQNLQK
                     AYEEVTGEEAKLLAIGGGTYAKAIPNTVAFGPEFPGEPMLAHKANERLSLESLEKMTL
                     IYAYAIYLLSK"
     gene            complement(2874..5984)
                     /locus_tag="PLW82_00470"
     CDS             complement(2874..5984)
                     /locus_tag="PLW82_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ45785.1"
                     /translation="MRIFEVGIENFGLFNDCTIHLSPKMNIIQGENEAGKSTLLTFVR
                     WVLFGFARNEKLVPFSGGDPSGYLLVDLDGKECVIRRQGKSLKGELSIVVDGKNLTGK
                     EAEHFLAKLLGSITDNVYKNVFAFGLGELQQISTVEDDQEVSSFLYSSGLYPGKLPLS
                     AITKELEDKSKALYSPSRHAKNPLINQELRSWEEIEDKLGEFKNDPEKYNSCLKEIEE
                     TGKELTHISERIRTLQEKRDWLGVLSKGRPTWSKIQEIKTLMELIEVDDEFPAESLGQ
                     VEKLKAEIEECENRLLALNADLSTHKEELNALVLDEKILENAAEIEFLSGELSRFTEW
                     KSGVQGLQDEITALEESLEKQIVGRLGNGWNLDKLKEEERTFSYEINDTVRKTEERFK
                     DQEFAIKQAGEKIEAADTKKRTLDQEEETLQEELQRLDVIDNFPEIKDAFETYRHLAP
                     SRAFLKGQIEAQKEPKAKPKKLSPVLFLIPAVLLAVLSGVFGLKANWPLAVAGATGAA
                     FSIGLQIVASRRYKKALVAWQEQESKRLEELEKLLDEMKKIDAKLELAKEVLPSSASS
                     DQANLIDLIQKHIEAEEDKWQKREELQRKLGHLATEKAKCEDERKAAEAALKEALKLE
                     EESRSQWEAFLRENDLPQMEPLNILDWLSAYRELKTRVDELRQKAVQKNAVQANIKQF
                     TEKAENLALRLGKNLENVEIDVVSWKKELSENNDTLLKKERLETEVKRIETQIKIEAE
                     KKKGKSEELDGILEKAKVSTVEEFRTLAKRKTDKDELRKQLALHQSNLISLVSGEESR
                     QKLLADLASFSEAELQKDINDTDLALASARTEQNQASEKLGRLQEQRSQLETGRQTEE
                     LLRQKASTKQKLSEYAKQWSQLVICRYLIEKARATYEREKQPGVLKTASTYFEKITNG
                     NYIKVMSPLTEQRFEVLSRDRKVKGTNELSRGTLEQLALCLRLGLILEFAKHQTPLPL
                     VIDDILVNFDPNRAANTLRILADLESMHQVLFFTCQTHLAGLCEDNNIEFKLTVLKNG
                     KVVSSA"
     gene            complement(5981..7183)
                     /locus_tag="PLW82_00475"
     CDS             complement(5981..7183)
                     /locus_tag="PLW82_00475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOJ45786.1"
                     /translation="MFRFIHAADLHLGNSGPSTSGFSKQWADLIYKATTTAFSNLIDK
                     AIELNVDFVILAGDVLDTANPNLNSQLNLFAGLNKLSRAGIQVFMAGGNHDPLDLKKA
                     QTTLPEGVHLFGERAETIPFVKGGVTLAEITGISFPVAKVSDDLSRQLVPGTKGFQIA
                     VLHSNVKGAGKKENYAPSKLEYLTQSGFDYWALGHVHTRAVLSEKPHVVYPGTTQGTH
                     VNETGEKGCYLVEVENDSVSLQFIPTSAYTWEKLELEAPKTLNELTELLEGLDELASG
                     AKKVYRLKLTGMTPLYETLITDLKDLEERFKEGKHYVLESVELDILPDVDLEEVKLEE
                     SLPGEFLREAERLMALGVSSELKEQLAPLYDRLGGILDDLDEEEVKSIINGSIALGIE
                     LFWRGESR"
     gene            complement(7441..8091)
                     /locus_tag="PLW82_00480"
     CDS             complement(7441..8091)
                     /locus_tag="PLW82_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45787.1"
                     /translation="MKKKLLGLLVVVLLLSAGAFAEVIYSQDFEDNKMPDDFLVVEGS
                     WRVLNGQLIGESPNSSVQGRVVFGPVMDSFVYTVDVTILNVVESTRWFSIFFRSTEDG
                     SAPYHMFTIRQGASAHNGTELAFRHPQGYWDVRRTKPYERDFGFRETFTLTVAVDGDL
                     FLYFINGELQFAAYENGYRDEGIFGLHVNGCRVAFDNIKIEKYEHSKFRQYEREAY"
     gene            8292..>8952
                     /locus_tag="PLW82_00485"
     CDS             8292..>8952
                     /locus_tag="PLW82_00485"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOJ45788.1"
                     /translation="MTETIRIGIVGFGNLGKGALQALKYSSDMEIVGIFTRRDPKSVK
                     VDGHVPVYHMDDVQDFVDKIDVMLLCGGSATDLPEQGPVLAKLFNTVDSFDTHARIPE
                     YFAKMDEASRSGGKTSIISVGWDPGLFSIMRAFTEAILPEGDTYTFWGKGVSQGHSDA
                     IRRVPGVKAGIQYTIPLDDALEQVRSGSRPVLSTREKHRRECFVVAEDGADKAKIEET
                     IK"
BASE COUNT         2067 a         2323 c         2019 g         2543 t
ORIGIN      
        1 aaaccaaatc ggccagggca aatacctcat agacatccaa agagaagacg ttctcccagg
       61 agatctcatc ttctttgaag gctacggcca cgtcggcctt gccatttccc actacgagtt
      121 catccacgcc acaacctaca gcaaacccgt tgttcaagtg tcagaaatcg actgcccgat
      181 ctggcgagat agaatcattc agtacagcag atatagatta gaaaagtaga acctgcgccg
      241 gcaggttcta ctttttcttc tcaaatgccc cttggtaata atcaataatc ctatccagaa
      301 aatactcaaa gtcaaagtac ttcttggcaa actcataact tttatactgc aaaagcctta
      361 acgcctcgcg attctctatc agcggagcta gatcgctcac gatagtcctc ggagacggcc
      421 acaccttcga ctcgcgtccg gtaaactcgg gaaccggggg aagcaggtcc ggctggacta
      481 tgccatctat gaatttaccc attacataac ctacgtttgc tgcagccatc ccctctcgga
      541 ttgccatacc ggttccgatc acgatatccg aatctcccag ccacttggct ctgtctgtct
      601 gccagccaag ctgcttgatg tagttgacct catcgaagat tccccgggga atattgccaa
      661 tgactctcgc ttctaccggg cgatctttcc taagagtctt gatggcattg ttaagcgcga
      721 tcaagcccac acgcttactg tcggcgtatc tgcccaggta tgtgagcatg accttggtgg
      781 tgtgcttctt ttgtggttta tgaacatcca gttcaacagc gttccccaga actatgatgt
      841 tttcagcaaa actcctatac ggtgcagcaa acacctctgc cacagacagc accaagtctg
      901 cctgctgcat aacatctgtg taacgctcca gtccagtgcc gtggatagtt acagcaaagg
      961 gacaaccgct tgcctttgca agttccaaac cttttctcca cgtgagagcc gagtgtgcgt
     1021 ggattatggt atcctgcgac accttcttcg gtagggatcc aagtacattt actccagctt
     1081 ctgcaagtgc gttcaactgc aacctgaaag cttcgataat cgccgtacgg gacctgagat
     1141 acctgaagtt gttgttgaag tagatgctaa catcgaagcc ccgcctggca agggctttcg
     1201 cgagcatttc tacatgtaca gattggccgc agcggaaata gtggttgagc agcaaaacct
     1261 tcatacactc acctccgtta aatcagtcta tgcggaggca ggttttcgtg tcgctaaagg
     1321 tcctgtgttc taaagcccca tttcatctgt gtttgaagaa atagcacttc gagtcataaa
     1381 ccgcagtaaa aaagagaccc ttttacgagc ctctctttac atctgtatta cttgcttaaa
     1441 agataaatag cgtaggcata gattagtgtc attttctcca aagactctag actgagcctt
     1501 tcgttagctt tgtgggcaag catcggctct cctggaaact ctgggccaaa tgccacggtg
     1561 ttggggatgg ctttggcata ggttcccccg ccaatagcca gcaacttggc ttcttctccg
     1621 gtaacttcct cataagcctt ctggaggttt tggatcagtg ggtggtcgtt ctccaccaaa
     1681 agtggtgcct ttgtggtatc tccgccaacc gataaaggtt ttacctccaa accgggaaca
     1741 aggtgccgct taactctctc tacgatctct tcgatggtca cgccgtaagg atagcggatg
     1801 tcgatagtga ctttgcactg atcttgagtt acccgcaaaa ccccagggtt catcgaaagc
     1861 ttgcctgcga tctcatgctc gaaatctatg tcaagagccg caccgtcggg gctgtggcca
     1921 actttggcaa agaactgaac cattggatgt tgaacaccca ttttgtccaa gccgatgaaa
     1981 aggtcgatga tagcgttgtg tccgagctgt ggtgttgaac catgagcttc tagacctttc
     2041 gcccaaacaa acttgaagtt accttcaggt gaaggtccaa gaagtgcttc tggttcggca
     2101 aggtctgcct ctttgacctc ctcaagtgga gcctccactt tcaggcccgc aggtactttg
     2161 gcaaaagctc tgtttggcac tacgtttgcc cgctctcctg caaaagctct gaacttgtct
     2221 gtaagaggtc catggatctc tagatgcaga atgccttttt cgctttggat aatcgggaac
     2281 tgcccgtcag gggtaaagcc ataggtaggg atctcctcag agttgaaata ggcggtcata
     2341 catttccagt tggtttcctc tgcggtgcct acgatggtcc tgatgcgctt tttaggtttg
     2401 aagcccagat tcattaatgc cttctgggca tacaggcagg caatggtcgg tcctttgtca
     2461 tcgagggcac ctcttcccca aatgtagccg tcttccactt taccgctgaa aggatcgtcg
     2521 tcccaaccgg tacctgcagg aaccacgtcc aggtgtccca gaaccgctac gatctcatcg
     2581 ccgcttcccc attcagcgtg tcctgctacg ttccccacgt tcttgaacga gaatcccaac
     2641 tctctcgaaa tctcttccat cttcatcaga gctctatagc aaccctctcc ctggggtttt
     2701 tctggagact gggtcttggg atcttccacg ctgggaatct ttatcatctc actgatgctt
     2761 tcaatcatct ctggcatcag ttttttgatt tccccttgca gtttgttgag catctagtat
     2821 cacctcttca tgaattgcac ctaagcaaca tctaaaacaa ctaataccct gcatcacgct
     2881 gagctaacaa ctttaccatt cttaagaaca gttaacttga actcaatatt attatcctca
     2941 caaagccccg ctaaatgagt ctggcatgtg aaaaacagca cttggtgcat ggattctaag
     3001 tccgccaaaa tacggagcgt gttcgcagcc ctgtttggat cgaagtttac gagaatgtca
     3061 tcaatcacca gcggaagagg cgtttggtgt ttggcaaact ccaaaatgag ccccaaccgg
     3121 aggcagagcg ctaattgctc taaggtaccc ctgctgagtt cgttggttcc cttcaccttc
     3181 cgatctctgc tgagaacctc aaacctttgc tctgtgagag gggacatcac tttgatgtag
     3241 tttccgtttg tgatcttttc gaagtaggtg gacgcggtct tcaaaactcc cggctgtttc
     3301 tccctctcat aagttgctcg agccttttct atgagatacc tacagattac tagctgcgac
     3361 cactgtttgg cgtactcgct gagtttctgt ttggtcgatg ctttttgcct tagaagctct
     3421 tccgtttgcc tgccggtctc tagctgactt cgctgttcct gcagtctgcc caacttttcg
     3481 ctggcctgat tctgttcggt ccttgcggaa gctagagcaa gatctgtatc gttgatatct
     3541 ttttggagtt ctgcttcgct gaaagaggca agatctgcaa gaagcttttg tcttgattcc
     3601 tcgccggaaa ctagtgaaat aagattggac tggtgcagag ccagctgctt cctcagttca
     3661 tctttatccg tctttctctt tgccaaagta cggaactctt ctacagtgct gaccttggct
     3721 ttctcgagaa ttccgtcaag ttcttcgctc ttgcccttct ttttctctgc ttcgattttg
     3781 atctgggttt cgatcctttt tacctcggtc tcgagcctct cttttttcaa cagagtatcg
     3841 ttgttttcac taagttcctt tttccatgat acgacatcta tctctacatt ttccagattt
     3901 tttccaagtc ttagagcaag gttttccgct ttctccgtaa actgttttat gttggcttgg
     3961 acagcgtttt tttgcactgc cttctgccta agttcatcta ctcgggtctt gagttccctg
     4021 tatgcgctga gccagtccaa aatgttaaga ggttccattt gcggaaggtc gttttctctc
     4081 aggaaagcct cccactggct gcgggactct tcttctaact ttagggcctc ctttaatgca
     4141 gcttcagcgg cttttcgctc atcttcgcac ttcgctttct ctgtggcaag atggccaagt
     4201 tttctctgca gttcttcgcg cttttgccat ttatcttctt cagcttcaat atgcttttgg
     4261 attaaatcaa tgaggtttgc ttgatcgcta ctggcactgc tgggcaggac ttcttttgct
     4321 aattccagct ttgcgtcgat ctttttcatt tcatccagca gcttctccag ttcctccagt
     4381 cgcttgcttt cctgctcctg ccaagctact aaggctttct tgtaacgtct gctagctaca
     4441 atctgcagac cgatgctaaa tgcagcacct gtcgccccag ctactgctag tggccagttg
     4501 gccttcagtc cgaacactcc agatagaacc gccaaaagca cagccggtat caggaacagg
     4561 accggactta gttttttagg ctttgccttt ggctcttttt gcgcttcgat ctgccccttt
     4621 aaaaaagctc ggcttggtgc aagatgcctg taggtttcaa aggcatcctt gatttccggg
     4681 aagttgtcta ttacatctaa acgctgcagt tcttcctgca gtgtttcttc ctcttggtca
     4741 agggttcttt tcttagtgtc tgcggcctcg attttttccc cagcctgctt gatggcaaac
     4801 tcttggtctt tgaatctctc ctcagttttc cttaccgtat cgttgatctc gtaggagaag
     4861 gtgcgttctt cttctttcaa tttgtccaga ttccagccgt taccgagcct tccgacgatc
     4921 tgcttctcca aagactcttc cagagccgtg atctcatctt gaagaccctg gactccagac
     4981 ttccactctg tgaacctgga aagctcaccg ctcaaaaact cgatttctgc tgcgttttcc
     5041 aagatttttt catcaaggac aagtgcatta agctcttcct tgtgtgtgga aagatcggcg
     5101 ttaagcgcaa gcaggcggtt ttcacactct tcgatctccg ctttaagctt ttctacttga
     5161 ccgagggatt ctgccgggaa ttcgtcgtct acctcgatca gttccataag ggttttaatc
     5221 tcctggattt tgctccatgt aggcctccct ttgctgagaa ccccaagcca gtcgcgcttt
     5281 tcctgcagcg ttcggatcct ctcgctaatg tgggtaagct ccttccctgt ctcttcgatc
     5341 tcttttaagc agctgttgta cttttcagga tcattcttaa actcgcccag cttgtcttcg
     5401 atctcttccc aagagcgcag ctcctggttg atcaaggggt ttttggcatg ccgcgaaggc
     5461 gaatacagtg ctttgctctt gtcttccaat tctttggtga tcgcagacag aggaagcttt
     5521 cccggataga gaccgctgga gtaaaggaag ctgcttacct cttgatcgtc ttccactgtg
     5581 ctgatctgct gcagctcgcc cagcccgaag gcaaagacgt tcttgtagac gttgtcggtg
     5641 atggagccta ggagttttgc caggaagtgt tccgcttctt tgccggttag gtttttcccg
     5701 tccacaacaa tcgaaagctc gccttttagg ctttttccct gtctccgtat gacacactcc
     5761 ttaccgtcaa gatccactaa aaggtacccg ctagggtcgc caccgctaaa gggaacaagt
     5821 ttttcgtttc tggcaaaacc aaaaagtacc cagcggacga aggtgagaag tgtactcttc
     5881 cctgcctcat tctcgccttg aataatattc atctttggag aaaggtgaat ggtacagtcg
     5941 ttgaaaaggc caaagttttc gatgccaact tcaaagattc tcatcggctc tcacctctcc
     6001 aaaacagttc tatgcccagt gcaatgctac cattgatgat cgactttact tcttcttcgt
     6061 cgagatcatc taaaatgccg cctagccggt cgtagagggg ggcaagctgt tccttgagtt
     6121 ccgagctcac gccgagagcc ataagtcttt ctgcttctct aagaaactcc ccgggaaggc
     6181 tttcctcaag cttaacctct tcgagatcga cgtcaggcag aatatccagc tcgacggact
     6241 ccagaacgta gtgcttccct tccttaaagc gctcctctag atcttttaag tcggtgatga
     6301 gggtttcgta caggggagtc atgcctgtga gcttcaggcg atagaccttc ttcgcgccag
     6361 aggctaattc atctaagccc tccaggagtt ctgtaagctc attaagggtt ttgggcgctt
     6421 caagttctag cttttcccaa gtgtatgctg atgtgggaat gaactgcaga gacaccgaat
     6481 cgttttcaac ctcaaccaaa taacaaccct tttcgccggt ttcgttgacg tgggttccct
     6541 gggtcgttcc cggatacact acatggggct tttcagacag aacagctctt gtgtgcacat
     6601 ggcccagtgc ccagtagtca aagccgctct gggtcaagta ctccagcttg gacggagcgt
     6661 agttttcctt ttttcccgct cccttaacgt tggagtgcaa aacggcgatc tggaaacctt
     6721 ttgtgcccgg taccagctgc cggctgagat catctgaaac cttggcaaca gggaagctga
     6781 tgccggttat ctccgcaaga gtcacgccgc ccttaacaaa cgggatggtt tctgcccttt
     6841 cgccaaacag atggacaccc tctggaagag tggtctgagc ttttttcagg tccaaaggat
     6901 cgtggttgcc acctgccata aagacctgga tccccgcacg gctcagtttg ttcaatccag
     6961 caaaaaggtt tagctgcgag ttcaggttgg ggtttgccgt gtccaaaacg tcgcctgcca
     7021 gaatcacgaa atccacattc agttcaatgg ctttatcgat gaggttgctg aaggcagttg
     7081 tggttgcttt gtagatcaag tccgcccatt gtttggaaaa gcccgacgta ctcggaccgc
     7141 tgttacccaa atgaaggtct gctgcatgaa taaaacggaa cattcctctt cctcctgggt
     7201 tatgtattaa ttaagtgatt ctttaagtcg gcttctatcc ctctattttc cattccagcg
     7261 cacacaggct ggagaagaat taaggggccg agctgcaagg gaatgagaaa cataaaatgc
     7321 ccgatgtctc tataagctag gtggacagta agtgtcgaca cctacaaaaa aacgcctata
     7381 tttatcaggc gatgataaat ataggcatcg gcgattcaga tccaaaagtc gcgttttgaa
     7441 ttagtaagct tctctttcgt actgtctgaa cttagagtgt tcgtatttct cgattttaat
     7501 gttatcgaaa gcaactctgc agccgttgac atggaggcca aagatgcctt catcccggta
     7561 tccgttttcg taggctgcaa actggagctc accgttgatg aagtagagga atagatcccc
     7621 atcaactgca actgtgagcg taaatgtctc tctgaagccg aagtctcttt cgtacggttt
     7681 ggtcctcctt acatcccagt acccttgggg atgtctgaag gcgagctccg taccgttgtg
     7741 agcactagcg ccttgtctga tggtgaacat gtggtaaggt gcagaaccgt cttcagtcga
     7801 tctgaagaag atcgagaacc agcgggtgga ctctactacg ttaaggatgg tgacgtctac
     7861 ggtatagaca aaactatcca ttacaggacc aaaaaccacg cgtccttgaa ctgagctgtt
     7921 cgggctttca ccgatgagct gtccgttgag cactcgccaa gatccttcaa caactagaaa
     7981 atcatcgggc atcttgttgt cttcaaagtc ctggctgtag ataacctctg caaaagcccc
     8041 agcagaaaga agcaaaacca ctactaaaag ccctaatagt tttttcttca tgtgtataac
     8101 ctccctcacc ctataatccc aatagggaaa ttcttcattt tttcgaattt acctgcaagt
     8161 ttatcccaaa tgccataaat accgtgaaga aaaaagcaca gtatacaaaa gaccgaaatc
     8221 ttgcgcgtct acggagaaaa agataacatg gaagaaaggt ttttcattct gtctttttgg
     8281 aggagatcat catgacagaa accattcgca ttggcatcgt tggtttcggt aatctaggca
     8341 aaggagcact gcaagcgctg aagtacagca gcgatatgga gattgtgggg attttcaccc
     8401 gccgtgatcc taaaagcgtc aaagtagacg gacacgttcc ggtatatcat atggacgatg
     8461 ttcaggactt tgttgacaaa atcgacgtta tgctcctctg cggaggttcg gcaacagacc
     8521 tgcccgagca aggaccggta ctagcaaagc ttttcaacac cgtcgacagc tttgacaccc
     8581 acgctagaat acccgaatac tttgccaaaa tggatgaagc ttccagatct ggtggaaaaa
     8641 ccagcatcat ctctgtaggt tgggatcctg gtcttttttc catcatgcgt gcctttaccg
     8701 aagctatcct gccagaaggc gatacctaca ccttctgggg caagggagta agtcaaggcc
     8761 actccgacgc catcaggcgc gtgcccgggg tgaaagcggg gattcagtac accatacctc
     8821 tggatgatgc cctcgagcag gttcgaagcg gctcaaggcc tgtgctttct acaagagaga
     8881 agcaccgccg ggagtgcttt gttgttgcag aagatggcgc agacaaagct aagattgagg
     8941 aaaccatcaa aa
//
LOCUS       DAOSDA010000009         8930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_5118_length_8930_cov_3.199549, whole genome shotgun sequence.
ACCESSION   DAOSDA010000009 DAOSDA010000000
VERSION     DAOSDA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8930
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5118_length_8930_cov_3.199549"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLW82_00490"
     CDS             complement(<1..757)
                     /locus_tag="PLW82_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOJ45789.1"
                     /translation="MNEEKVLYKIASELSTTYMRTAEPRKLLPNGDDVIAVLTEIQYL
                     LYPQYFAGVSGEMDDQVCYLERLGVLYWRLTRLIHSSIAERCKPECKISIEGCEELAE
                     SREKALAIICQLPEIKRLLDLDAEAAYKGDPAARNIDEVILAYPGFYAIFVHRVAHEF
                     RKLGLDLMARIMSEYAHSKTGIDIHPGATIGESFFIDHGTGVVIGETTEIGKNVKIYQ
                     GVTLGALSFPKDHDGNIIKGQKRHPTIEDNVTIY"
     gene            complement(790..3525)
                     /locus_tag="PLW82_00495"
     CDS             complement(790..3525)
                     /locus_tag="PLW82_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45790.1"
                     /translation="MVRRSFMALIAVLVFANFAASQEIDLLDFAAPQVVIQGFLDPDT
                     STIAGTEKIQLTNTGESPLTEIVFLLPANLGSEPNHYLSELFGSKGYYKNFDSSHTYV
                     SGVRVNGKPAEFSLEEGPSVLQTYSLDSVKLIVQLGEILPGEETSVEIDFLTKIPYKR
                     GDFGLSANGIFYLNGRFYPELLNPWDPLKRTVKRYEADFLLPSTLVVASNADYQYETS
                     SGGIYKKVSLRYDYPVFDLAFAAGPGLFVKETTAGGVELYTYYLKDHEKQAVFFMQTA
                     ADVLQYYGENFGPYPKKRLTIVEGSALDVGAKAQGMIIVPESYYRYYNAGSSSADGIL
                     SAKVAADLAYSWFAHVSSTLDLWEGTESAWLNDAIRNYAASAYFDSRGLGEEVTFWDR
                     LGFSMLKPDYSMDFGKNLELAYLEAVRFQGRDEPVTREYASLENPEVWETQIATKGYL
                     LLRTLESFGGKERIFQLFKRTSELGGVGSFEVVTGERFYALAEEVFSDQGFPRFWQAF
                     VLSDAQEDLEILSAEQVESSGGYRINLEIGSNGAVFWPVEVLLEYRDGSSETVLFDLT
                     ENTLTVWSNSPLKRATVDPRGLVLDGERLNNSYPRKANFVIMSEISPDSTTYSLAPVL
                     EIRPEENTVNAGFALGYYNRIYNQLQFGFYYSTGQNRFLSEIEYLRNLPSGQLSFGAL
                     LSDQKPEEVGLGFEIRQKVKKNTGYAAYHNIDWFKGSFSLTYYPELNDYNLSLVAAYD
                     FSLLQGRNVTSTFDLSKRGGRLSIEVESIKAGPFAGFIRTRLNGQTSFQSPLKRAVTT
                     MHDVPEKTGDHALTFSASLSVPLILDKELFAIGPLVWEDAEGRLSVVAGNAWQKGSFA
                     LKATQAYLKGELLLGFNFLAKPFAVRISMARDLLGPNVYSYISLSSRFYREVLGK"
     gene            complement(3645..4373)
                     /locus_tag="PLW82_00500"
     CDS             complement(3645..4373)
                     /locus_tag="PLW82_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOJ45791.1"
                     /translation="MKVDVKLSPLAAEARTHLDATVIVVDVLRASTTIITALANGARN
                     VVPVLTPDEAATLKLRYDSANVLLGGERGGVKIQGFDLGNSPLEYTEETVSGKTIVFT
                     STNGTEAIGKTTSAQTVLIGAYLNLEAVARFAVELGKDVIIMCAGNQGSFALEDAACA
                     GAFCMAISRLTSVEESDSARAARMIASSFDTVIDTFHASAHGRNLVNLGFSEDLEYCA
                     RFNTTDVIPEYYNGHLIIRQKKSH"
     gene            complement(4401..5360)
                     /locus_tag="PLW82_00505"
     CDS             complement(4401..5360)
                     /locus_tag="PLW82_00505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOJ45792.1"
                     /translation="MERKKVLITRQLPGDAVEKLKEECDLLCDPPSDQMPRNQLLELI
                     PEADALICLLSETIDREVISRAPKLKVISNYAVGYNNIDVAFATERKIYVTNTPDVLT
                     EATADLAWALLLAAARRVVEGDEMVRQNRFTGWAPDLLLGLDVAYKTLGVVGLGRIGL
                     AVARRAKGFGMRVLYWSKNRKEQWEEQFGLEYQPLDTLLQTADFVSLNVALTPETHHL
                     IGEKEFSLMKNTAILVNTARGPVVDEAALAAALREKKIWAAGLDVYENEPEVHPDLLK
                     LDNVILAPHVGSGTIDTRAKMAEMVVSNVLMALKGQRPTNAIN"
     gene            complement(5520..6224)
                     /locus_tag="PLW82_00510"
     CDS             complement(5520..6224)
                     /locus_tag="PLW82_00510"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOJ45793.1"
                     /translation="MIKYGFLAGLILLPIILVVVAIIYGGYIAVVVSIAKTNPELAEA
                     TAATGETVQISVANYLKPLLQSWKAVLIGIVPGMLATGVLGSAFAHFYAKGIKRDKPF
                     ALFFAWGLLFAIFLIYALFKIQLAWYFNIFLAGFLAGVIGYTLVAFWRAMYTRFMFRY
                     SQGVLMNIFLALEEAMDSDPNLRRVYIIDWDREAGKVTVGGAVDNLKRKEKVEEIMRS
                     VEGVKDVENRCRHRYE"
     gene            6820..7014
                     /locus_tag="PLW82_00515"
     CDS             6820..7014
                     /locus_tag="PLW82_00515"
                     /inference="COORDINATES: protein motif:HMM:NF019199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOJ45794.1"
                     /translation="MAKDVKCEVDTCHYWAQGNKCNAEEILVNYNFQTTGRSDVEFGM
                     IEKKNTTDATCCETFRPKAK"
     gene            complement(7089..7955)
                     /locus_tag="PLW82_00520"
     CDS             complement(7089..7955)
                     /locus_tag="PLW82_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOJ45795.1"
                     /translation="MGYRVLEKKDLAPGIYSLKVEAPLIAKKAQPGNFVILRYKYTGE
                     RLPFTLVSWDPDEGWVQLIIQEVGKSTREMAQLTAGDEILDLLGPLGKPYPMQKYSRP
                     VVGVAGGLGAAPLYPILVGLQKMGNQIITITGARNKELFILREELQQISREVLVATDD
                     GSEGRKGFVTDVLKDLLDKEQVGQVIAIGPVPMMAAVSRVTKQYNTPTMVSLNALMVD
                     GTGMCGGCRMTVGGKTKFTCVDGPDFDGHQVDFDEIFRRQRFYRNQEVESLEIFEHAC
                     RLDSAIAKLEDK"
     gene            complement(7989..8402)
                     /gene="ndk"
                     /locus_tag="PLW82_00525"
     CDS             complement(7989..8402)
                     /gene="ndk"
                     /locus_tag="PLW82_00525"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOJ45796.1"
                     /translation="MEKTLVIIKPEGVKRGLVGECISRFEKKGLAIKQLAVKQIPAEL
                     AKIHYAHLADKPFFQGIIDAFTASEVVLMILEGECAVAVVRKMIGATNPLEAEPGTIR
                     GDFGGALPYNIIHASDSVESAEIEIKRFFSDSWQD"
     gene            complement(8408..>8930)
                     /locus_tag="PLW82_00530"
     CDS             complement(8408..>8930)
                     /locus_tag="PLW82_00530"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOJ45797.1"
                     /translation="QDKKNLPQGIIRQASDIIQKALTFLPEFREANNILTYSSLPWEV
                     DTEFVWEYKNTKNLIFPKVDVEKKVLRLFQVHSKEDLMPGVKGILEPVGGVEVDPEVP
                     DFIIVPGVAFDRRGNRLGHGAGYYDKLLKHSLGLKVGVVLERYCFNENIIPVEAHDVP
                     VDAVITEAGVFWR"
BASE COUNT         2213 a         2353 c         1963 g         2401 t
ORIGIN      
        1 catagatcgt cacgttgtct tcgatagtgg gatgacgttt ttgccctttg atgatattgc
       61 cgtcatgatc tttaggaaag cttaaagccc ccaaggttac cccctgatag atttttacgt
      121 tcttgccgat ctctgtggtc tcgccgataa ccactccggt accgtggtcg ataaagaagc
      181 tctcgccaat ggttgctcca ggatgaatat caataccggt cttggaatga gcgtactcgc
      241 tcataatcct tgccatgaga tccaacccga gctttctgaa ctcgtgtgct acacgatgga
      301 cgaagatggc gtaaaagccc ggataggcta aaatgacttc atcgatgttt cttgctgcag
      361 gatcaccttt gtaggcagct tccgcatcca aatccaggag ccttttaatt tcaggcagct
      421 ggcagatgat cgccagggct ttctcccttg actcagcaag ttcctcgcat ccctcgatgc
      481 tgattttgca ctcgggcttg caccgctctg caatggagct gtgaatcagc ctagtaagcc
      541 tccagtataa gacacctagt ctttccagat agcagacttg atcgtccatc tctccgctta
      601 cgcctgcaaa atactgaggg tacagaaggt attgtatctc ggtcaagaca gcgatgacat
      661 catcgccatt tggcagaagc tttctcggct ctgcagtgcg catataagtt gttgaaagtt
      721 ctgatgctat tttgtacaga accttttctt cattcattta gattctgccc tcctatatct
      781 acttaagcct cacttaccca aaacttcccg gtagaaacga gaagacaggg agatatagct
      841 atagacgttt ggtcccagca ggtcccttgc catgcttatc ctcaccgcaa agggttttgc
      901 caggaagtta aagccgagaa ggagttcccc cttaagatag gcttgggtcg ctttcaaagc
      961 aaagcttccc ttttgccagg catttcccgc cacaacagaa agcctacctt cagcatcctc
     1021 ccagaccaat ggaccgatgg caaatagctc tttatctaaa atcagcggaa cggaaagcga
     1081 cgcgctgaaa gttaatgcat ggtctcctgt tttctcgggt acgtcgtgca tggttgtgac
     1141 cgctcttttc aaaggactct gaaaagacgt ctgcccatta agcctagtcc tgataaaccc
     1201 tgcaaaaggt ccggccttga tagattctac ctcgatggac agcctgcctc cacgtttaga
     1261 caggtcgaac gtagaggtca cgtttctccc ctgaagcaat gaaaagtcgt aagctgccac
     1321 gagactcagg ttgtagtcgt tcagctctgg gtagtaagtc agactgaagc tccctttgaa
     1381 ccagtctata ttatgatatg ctgcgtatcc cgtgtttttc ttgaccttct gcctgatttc
     1441 aaaacccaag cccacctctt caggcttctg gtctgaaaga agcgccccaa aagacagctg
     1501 cccgctgggc aggtttcgca ggtattcaat ctccgacaag aacctgtttt gtcccgttga
     1561 gtagtagaag ccaaactgca gctggttgta aatgcggttg tagtagccga gcgcaaaacc
     1621 tgcatttacc gtgttttcct caggcctgat ctccagcacg ggagccaagc tgtaggtggt
     1681 gctgtcaggg ctgatctcac tcattataac aaagttggct tttcttgggt agctgttgtt
     1741 caaccgctcc ccgtcgagga caagtcccct aggatcgaca gtggctctct tcagaggaga
     1801 attgctccaa acagtgaggg tgttctcagt aaggtcgaac aacaccgttt ccgaagagcc
     1861 atccctatac tcaagaagca cctccacagg ccaaaatacc gctccatttg aaccgatctc
     1921 caaattgata cggtatcctc cgctagactc tacctgctcg gcactgagta tctccaggtc
     1981 ttcttgggcg tcgctaagta caaaagcttg ccaaaatctc ggaaaaccct gatcgctaaa
     2041 gacctcttct gcaagagcat agaagcgctc gcctgttaca acttcaaagg agcccacgcc
     2101 tcccaattca gaggtccgct taaagagctg aaagatgcgt tccttacctc cgaagctttc
     2161 caaagtccgt agcaacaaat aacctttagt tgctatctgg gtttcccaga cttccgggtt
     2221 ttctaaagaa gcatactccc tagttacagg ttcatctctg ccttggaagc gcaccgcttc
     2281 taagtacgca agttcaaggt ttttgccaaa atccatagag tagtctggct ttagcatact
     2341 gaaacctaaa cggtcccaaa aggtaacttc ttctcccaga cccctactgt cgaagtaagc
     2401 ggatgcggca tagttcctaa tcgcgtcgtt aagccaggca ctttcggtac cctcccagag
     2461 atctaaggtc gaacttacat gggcaaacca ggagtatgcc aaatctgctg caacttttgc
     2521 acttaagatg ccgtctgcac tgctgcttcc tgcattgtag tagcggtaat agctttccgg
     2581 aacaatgatc attccctgcg cttttgcacc cacatcgaga gcagatccct ccacgatggt
     2641 caggcgcttt ttggggtaag ggccaaagtt ttcaccgtag tactgcagaa catcagctgc
     2701 agtctgcata aagaagactg cctgcttttc gtggtcttta aggtagtagg tgtagagctc
     2761 tacccctccg gcggtggttt ccttaacaaa aagcccggga cctgcagcaa aagctaggtc
     2821 aaaaacggga taatcgtatc taaggctgac tttcttgtag atgccccctg aagaggtctc
     2881 gtactgatag tccgcgttgc ttgccaccac cagtgtggac ggcaggagga aatcagcttc
     2941 ataccgtttt accgttctct ttaaaggatc ccatggattt agaagttctg gatagaaacg
     3001 cccgttcaaa tagaaaatac catttgcgct gaggccaaaa tccccccgtt tgtaagggat
     3061 tttggtgagg aaatctatct ccaccgaggt ttcttcccct ggtaggatct cgccgagctg
     3121 aacaatcagc tttaccgagt ccagggagta ggtctgcaaa acagaaggtc cctcctctaa
     3181 ggagaactct gctggctttc cattgacccg gactccgctt acgtaagtat ggctgctgtc
     3241 gaagtttttg tagtaccctt tagagccaaa aagctcgctg agatagtggt ttggttcaga
     3301 gccgaggttt gccggaagca aaaacacaat ctctgtgagg ggggattccc cagtgttagt
     3361 caactggatc ttctcagttc ctgcgatggt gctggtgtca ggatccaaaa aaccctgaat
     3421 aactacctga ggagctgcaa aatccagaag atcgatctct tgtgaggccg caaagtttgc
     3481 aaaaacgagc acggcaatca gtgccataaa actgcgtcta accactttcg actcctcccc
     3541 agtcccttct catatgtgaa aaatccagcc tacctcggca aaaatgctca ggtaagctga
     3601 gaaaacggcc tgtgttttca ttgtagtata aaacaaaaga agcctcaatg agacttcttc
     3661 tgcctgatga ttaagtgtcc gttgtagtac tccggtatca catctgtggt attgaacctg
     3721 gcacagtact ccagatcttc actgaagcct aggttcacta agttccgccc gtgtgcactg
     3781 gcgtggaagg tatcgatgac cgtgtcaaag ctggaggcta tcatccttgc agctctggca
     3841 ctatccgact cttccaccga agtcagcctg gatatggcca tgcagaaagc tccggcacaa
     3901 gctgcatctt ccaaagcaaa gctgccttgg ttccctgcgc acataatgat cacgtctttc
     3961 cccagttcga ccgcgaacct tgcaactgct tccaagttca agtaagcacc gattagcacc
     4021 gtttgagcgc tggtagtctt tccgatagct tcagtgccgt tcgtcgaagt aaagacgatg
     4081 gtcttgccgc taactgtctc ctctgtatac tccagcggcg agttgcccag gtcaaaacct
     4141 tggatcttta ctccgcctct ttctccgcca agcagtacgt tggccgagtc gtatctcagc
     4201 ttcaaggtag ccgcttcatc gggagttaag accggaacga cgtttcttgc gccgtttgcc
     4261 agtgctgtaa tgatcgttgt tgaagcccga agcacatcta ctacaatcac tgtcgcatct
     4321 agatgggtcc ttgcttccgc cgctaagggt gaaagcttaa catccacttt catcttgcaa
     4381 tcaacctacc attctcccac ttaattaatt gcattcgttg gtctttgacc tttaagggcc
     4441 atgagtacgt tggagacaac catttctgcc atttttgctc tagtatctat ggtgccgctt
     4501 ccgacgtgtg gtgccagtat cacattgtcc agtttcaaaa ggtcgggatg tacctctggt
     4561 tcgttttcgt atacgtccaa accggcagcc cagatctttt tctctcttaa ggccgctgcc
     4621 agagcagctt catctaccac cggtcctctt gccgtattta ctaatatagc agtatttttc
     4681 atcagtgaaa actctttttc tccgatcaga tggtgagtct ccggagtaag agcgacgttg
     4741 agggacacaa aatctgcagt ctgcaggagg gtgtctagag gctgatactc cagaccgaac
     4801 tgctcttccc actgctcttt gcggttcttg gaccagtaga ggacacgcat accgaaacct
     4861 ttagctctcc ttgccacagc aagtccaatt ctgccaaggc caacaacgcc tagggtcttg
     4921 taagcaacat ccaaaccaag gaggagatct ggagcccagc cggtaaaccg gttctgcctc
     4981 accatttcgt ctccttcaac cactcttcta gccgctgcca aaagcagtgc ccaagcaagg
     5041 tctgctgtgg cttcagttag cacatctggt gtgtttgtta cgtaaatctt tctttcagta
     5101 gcaaatgcca catcgatgtt gttatatcct acagcgtagt tgctgatcac cttcagcttg
     5161 ggagccctag agatgacttc tctgtcgatg gtttcgctta gaagacagat cagggcgtcg
     5221 gcttcgggaa tcagctccaa tagctggttt cttggcattt gatcactagg tggatcgcac
     5281 aataaatcgc attcttcttt cagcttttct actgcatctc ctgggagctg tctggtgatc
     5341 agaacttttt tcctttccac tagtcccact ccttcgtaga atcactaaat cttagtttac
     5401 cacatctaga gtcgggctgc atgcattatc agctgaaaaa gatgaagacc ttgtatgtag
     5461 ggcttcttca gaaaaaagaa agatagcccc cagtggagct atcttcactt tccattattc
     5521 tattcgtagc ggtgtctgca gcgattctct acgtccttga caccttccac gcttctcata
     5581 atctcttcta ctttttcttt acgcttgagg ttatctacag ccccaccaac agtgacttta
     5641 ccagcttcgc ggtcccagtc gatgatgtaa actcttctga ggttgggatc gctatccatg
     5701 gcttcttcta aagcaaggaa gatgttcatc agaacgcctt ggctgtagcg gaacatgaac
     5761 ctagtgtaca tcgctctcca gaacgccaca agggtgtagc cgattacgcc tgccaagaat
     5821 cctgcaagga agatattgaa gtaccatgca agttgaatct taaacagcgc atagatgagg
     5881 aagattgcaa acaacaaccc ccaagcaaag aacagagcga aaggtttatc cctcttgata
     5941 cctttggcgt agaaatgtgc gaacgccgag cccaaaactc cagttgcaag catgccaggg
     6001 acaataccga tcagaactgc cttccagctt tggagaagcg gtttcaaata gttggcaaca
     6061 ctaatctgaa cggtttcacc tgtagcagcg gttgcttcag cgagttctgg gttggttttt
     6121 gcaatactta caacaactgc tatatagcca ccgtatataa ttgccacgac caccaggata
     6181 attggcaata aaataagacc tgccaagaat ccatacttga tcatctcgga cggttctgaa
     6241 gtagataaca ccaatttcgt tttctcgtct tcatacaaac tagcatctaa atcgcgagcc
     6301 aaaaataaca cctccaccga tatggactac tagctataga ttcgcctctt ggagagaata
     6361 tcctccacaa ttaggttctc ttcacgaatt tatagctctg tcaattaatg catgtagttc
     6421 ttctagcaca gaccgcctgc attgggaaag aaagtttctc ctgcacgagc ctgcatcata
     6481 ggacctgcag cgatcgcttt ttcagccact tcgacgtttc ttgcaagttg aagtgcaagc
     6541 atttaccggg caaaccttta agctaccatg gtaccttttg gaaggtttca cactgcaaat
     6601 gaggtttcgt tggactatcg gacctgagat ctgcgaacct ctctcttcga aagcctttgc
     6661 tgcaaagcct ctgcctagag gctctgtcca cagaactaag caatctacca cagggagtca
     6721 cctcagtaga ttgctctttg acaattatac atcaaaaaaa acgtgccgtc agcatacatc
     6781 ttgtgctttg gggcagatta taagacgagg tgacttttca tggccaaaga tgtaaaatgc
     6841 gaagtcgata cctgtcacta ctgggcgcag ggaaacaagt gcaacgcaga agagattctc
     6901 gttaactaca acttccaaac caccggtagg agcgatgtgg agtttggcat gatcgaaaag
     6961 aaaaacacca cagatgcaac ctgctgcgaa accttccgcc caaaagcaaa gtgatctaaa
     7021 catgtgaaag gactggcctc caagccagtc ctttttcaaa tgtatttcgc tctgcactac
     7081 ctgctctttt acttgtcttc aagctttgcg atggctgagt ccagtctgca ggcatgctca
     7141 aatatctcca aagactctac ctcttgattg cggtagaaac gctggcgcct gaagatctcg
     7201 tcgaagtcca cctgatggcc gtcaaaatcc ggaccgtcga cgcaggtgaa cttggtcttg
     7261 cctccaacag tcatcctgca acctccgcac ataccggtgc cgtcaaccat aagggcgtta
     7321 agacttacca tagtcggggt attgtactgt ttagtgaccc tgcttacagc ggccatcatc
     7381 ggtactggtc cgatagcgat gacctgacct acctgctctt tgtccaggag gtccttcagc
     7441 acatcggtaa caaagccctt cctgccttcg ctgccgtcat cggtggcaac aagcacttct
     7501 ctggagatct gctgcagttc ctccctgagg atgaacagtt ctttgttcct tgcaccggtg
     7561 atggtgatga tttgattccc cattttctgc aaaccaacta aaatcgggta aaggggagcc
     7621 gctccaagtc ctcctgcaac ccctacaacc ggtctgctgt atttctgcat gggatagggt
     7681 tttcccagag gaccgaggag atccaggatc tcatcgcctg cagttagctg tgccatctcc
     7741 cttgtggact tacccacttc ctggatgatc agctgcaccc agccctcatc tggatcccag
     7801 ctgaccaggg taaaggggag tctttccccg gtgtacttgt atcttaaaat cacgaaattc
     7861 cccggttggg cttttttagc gattagaggc gcttccactt tcaagctgta gattcccggt
     7921 gctaagtcct ttttctctaa aactctatat cccacaatta caccttcttc acgcgttatc
     7981 tcaagctctt aatcctgcca gctgtcgctg aaaaaccttt tgatctcgat ttctgcactc
     8041 tccaccgaat cggacgcgtg gatgatgttg tacggaagag ctcctccgaa atctcctcta
     8101 atggttccag gttctgcttc aaggggattg gttgcaccga tcattttccg cacaactgcc
     8161 acagcacatt cgccttccaa gatcatcagc accacttcag atgctgtaaa agcatcgatg
     8221 atgccctgaa aaaaaggttt atcagccaga tgagcgtaat ggatcttagc cagttctgca
     8281 ggtatttgct ttacagcaag ctgtttgata gcaagccctt ttttctcaaa tctgctgatg
     8341 cattcgccga caaggcccct ttttacacct tctggtttga tgataactag cgttttttcc
     8401 atcttaacta cctccaaaac actcccgctt cggtaataac cgcatcaacc ggaacgtcgt
     8461 gcgcttctac aggtatgatg ttttcgttaa agcagtacct ctctaaaact acaccgactt
     8521 taagtcccag cgaatgtttc aagagtttgt cgtagtaacc ggcaccgtga ccgaggcgat
     8581 tgcccctgcg gtcgaaggcc actccgggaa cgataatgaa atcggggact tccgggtcga
     8641 cctccacccc acctactggt tccagaatgc ctttaactcc gggcatcaaa tcctctttgg
     8701 agtgaacctg aaacagtctg agcacttttt tctcaacatc tacttttggg aaaatcaaat
     8761 ttttggtatt cttatattcc cagacaaact cagtatcgac ctcccaggga agcgagctgt
     8821 aggtaaggat gttgttcgct tctctaaact ccggaaggaa tgtcaaggcc ttttgaataa
     8881 tatcacttgc ctgacgtata attccttgag gtaagttctt tttgtcctgg
//
LOCUS       DAOSDA010000010         8657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_5324_length_8657_cov_3.218438, whole genome shotgun sequence.
ACCESSION   DAOSDA010000010 DAOSDA010000000
VERSION     DAOSDA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5324_length_8657_cov_3.218438"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLW82_00535"
     CDS             complement(<1..313)
                     /locus_tag="PLW82_00535"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HOJ45798.1"
                     /translation="MELIYIISPIVGAVIGYLTNWIAIKMLFRPLKKIYIGPIALPFT
                     PGLIPKERQRIARSIADAVGQNLLSPDLLASEAVSEDKLHLIEGFLDKKLIDLKTSDK
                     TL"
     gene            complement(455..1735)
                     /locus_tag="PLW82_00540"
     CDS             complement(455..1735)
                     /locus_tag="PLW82_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ45799.1"
                     /translation="MKKRFAILLVILAVALGVNAVTLEVWWTRPNEIAQVIKDMANEY
                     FTPQTGIELNFTAMNYDDLWSKAMLALAAGDTPDLASFGSEWPVEFGMRGGLVDLRAE
                     HPEEFAEIEKKLYPGQMRSLEFMGTAFGIPVEFGFGLTYYRTDIFAENGWEYPTTWDE
                     VRALLPKFQANGMNLACISQYLLPDFQSVMTFIWQHGGVYVNDDGVSSGLNTPEAIAG
                     FKELMELFTVYKIPQAPVPMNTFASGEYPLVVGGNWNYSAVDQGFPQIRGKWSVGLTP
                     GTVRSDGFLSHSIYNGPLPIGIFKNSKYQKEGWEFIKWFMQDEVQAEYGRRVMQVMPS
                     GFFFSANMNVASKIDYFPEDHVKVMYEQSYHSMAPRYGLGSVVSQRHVTNAAMSVILQ
                     GTDPEKALLEAHDLVQAEMKRKQVEFARFISRLK"
     gene            complement(1798..3609)
                     /locus_tag="PLW82_00545"
     CDS             complement(1798..3609)
                     /locus_tag="PLW82_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOJ45800.1"
                     /translation="MKNELFLPMTLKEAQQWGWDSLDIVLVTGDAYVDHPSFGTALLG
                     RYLVSLGYKVGVIAQPNWRENEDFLKLGRPKLFFGVTAGNLDSMVANYTAANKRRRDD
                     AYAPGRKAGLRPDRATIVYSNKLKELFPDTPIVLGGIEASMRRIAHYDYWQNSVRRSI
                     LFDAKADLLVYGMGEKQIAAIAENFLMGLGVEACFGLRGTVHKEKDPYISKDTVVLPS
                     FAAVQDKKEFGRAYKLFYNELDPFEGRVVVQEQQDGWYAVVNPPEKPMTAEELDYIYG
                     LPFQRRAHPSYADQGGVPAVKTTQFSITTHRGCPGECYFCSLALHQGRVVQSRSEKSI
                     LQEAETIASDPEFRGTISDAGGPSANLYGTTCKLQARRGVCKHRSCMTPKICPNLEID
                     QQSYVKLLQNIAKVPSVKRVFVQSGVRFDAALKDAGFMKELIRNHVSGQLKVAPEHVS
                     SRVLRQMNKPAHPVYQEFCRKYSQISLDLGKEPYVIPYLISSHPGSTLNDAIELALYI
                     KKQGRFFEQVQDFIPLPLTVSGTMWYTGENPFTGEKVHIPDEEEKKMQRALLQFQDRR
                     NWPLVRKALRKAKREDLIGTGKNCLVPGETKSSAKKR"
     gene            3980..4324
                     /locus_tag="PLW82_00550"
     CDS             3980..4324
                     /locus_tag="PLW82_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45801.1"
                     /translation="MFDFLYNRRIPDGFRIPKWILKEYFGIEEEQLQVETNLILEKLT
                     QQIDDLSDDDHSPIAIWLAIQIMADQISETLSDSLEIYHKLAQGELTAREAIALTRLK
                     QIEREFFEEDDE"
     gene            complement(4354..5241)
                     /locus_tag="PLW82_00555"
     CDS             complement(4354..5241)
                     /locus_tag="PLW82_00555"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOJ45802.1"
                     /translation="MERERPFFRAVILPSEAGWKVKDVLKRHYHFSTSLIRNVKNRGE
                     LRINSQTSYFSYEVKAWDVVEVYLAVETKVTPEFSKIEVVYEDSDVLVVNKPAGMVMH
                     PRKRYVSGTLANYIAGHLVQKGERPSSYLVNRLDKETSGLVLAAKNPLAAVILSEEIG
                     EMEKHYLALVHGRVHSEAGVIDLGIVDPKEGVRREVGEDGRKATTKYEVLMRYTDHTL
                     VKAVLLSGRTHQIRVHFSSIGHPLVGDEVYGEGDEGLGCTFLHACHMAFPHPRTGTIC
                     QFDAELPVELKEYLNELHR"
     gene            complement(5309..5794)
                     /locus_tag="PLW82_00560"
     CDS             complement(5309..5794)
                     /locus_tag="PLW82_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOJ45803.1"
                     /translation="MNRNVVWLLVIAVVVVGLVVYFGSRVDDGVLDQKPEDIKEDVKD
                     RTEDLGKDLEDVGERLFDDGKYVGRSDADDRGNYGYIELTIKDGKIKEADYTEYQAPN
                     EEDPKDEDYPYKLVFDAIDKLEEELVRVQDPDKVDDVAGATGTSEKFRAAAKNALEKA
                     R"
     gene            complement(5833..6168)
                     /locus_tag="PLW82_00565"
     CDS             complement(5833..6168)
                     /locus_tag="PLW82_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOJ45804.1"
                     /translation="MDINLTTGQVKKLMAGKKESLLIVDVREPHEYAEGHIPGSILVP
                     LGDVAAHIDRFPKDKTILFVCRSGRRSLEAANLFLENGYTEVFNYQGGMLAWNGAVER
                     GLYKGNSEG"
     gene            complement(6396..7538)
                     /locus_tag="PLW82_00570"
     CDS             complement(6396..7538)
                     /locus_tag="PLW82_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ45805.1"
                     /translation="MRGKGNKPRYLLVKEGIVKDIKTGNYQVGEKLPGERKIAYQYNV
                     SQMTANRAIQELVQEGYLEREMGVGTFVVSRDPASEVPAKINIVLLGDFIRNQNDYEK
                     TLQTVHYLARDVYMGTMLRNLMEVGRFHKCEFSIITASTSFELEDYVARADRMRERFV
                     IVNPLYNWQECLDRIGRSGIPIVAINASWENMGVPFVDADNYLGAQMAISHLGELGHK
                     DILVFYSWPETANTQQRIKGAKDKMTELGIPYTEDQFININAIGIWALKLIQEKLEVV
                     LRRRRVTAIFAAGFELALAANSALKNLNISIPDEISLVGFDDSPAAIYLEPPLTTIKQ
                     PFYEIAEAAIDILINPENKSSKLIPAELILRRSTGLARKASTNPFP"
     gene            7720..>8657
                     /locus_tag="PLW82_00575"
     CDS             7720..>8657
                     /locus_tag="PLW82_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ45806.1"
                     /translation="MKKVVVLLLCLVISFSALAKERTLTIWAAWASEWVRIFQEMTDR
                     YFTPETGIEVEIMPLPWDSFEQKFLLAAATGDVPDVGLTGGLGPADLGVRGAVVDLKA
                     RFGQEVDELTSQFFPGLMRSLTFYDSVFGMPMIYGSYNLFYRTDILEELGLDIPNTWD
                     EVRAMIPKLQANDTNFAVAYGHQSTADFSMFLFQRNGDWYTPDLTKSALDTPEAIMAF
                     REFTDLFTKQNIPKEIQPYFQPFRTGDIAIQIGPTFDYANLQIAAPELKGKWGMSMVP
                     GTPNADGELNRAAYIGTMGIMIFEKSRDIPGAWEWV"
BASE COUNT         2094 a         2222 c         2006 g         2335 t
ORIGIN      
        1 caagagtttt gtcgctagtt ttcaggtcaa ttagcttctt gtccaaaaac ccttcgatca
       61 ggtgcagctt gtcctcagaa accgcttcgc ttgccaaaag atctggactg agcaggtttt
      121 gcccaactgc atctgcaatg cttctggcaa ttctctgcct ttctttggga atcaagcccg
      181 gagtaaaggg tagtgctatc ggaccgatgt agatcttttt cagcggtcgg aaaagcattt
      241 taatggcgat ccaatttgta agataaccta taactgcccc aacaatcggg ctgattatgt
      301 aaatcagctc catctgagcc acctctttta ccaggtcgaa tatctatact atgagtatat
      361 ccctggacaa gctataaaaa cattttaaca ccaaaacgct aaactccata gggcgggggc
      421 aaataaaaaa ggtggagctt cgctccaccg aagtttactt gagtctgctg atgaagcggg
      481 caaactcgac ttgcttgcgc ttcatctctg cttgaactaa atcgtgggcc tcaaggaggg
      541 ccttttcagg atcggttcct tggaggatta cgctcatcgc agcattggtg acgtggcgtt
      601 ggctcactac agaacccaac ccgtagcggg gtgccattga gtgataggac tgttcataca
      661 tcacttttac gtggtcttcg gggaaatagt cgattttgct tgccacgttc atgttggcag
      721 agaagaaaaa tcccgaaggc atgacctgca taacccttct gccgtactct gcttggactt
      781 catcttgcat aaaccactta atgaactccc agccttcctt ctgatacttg gagtttttga
      841 agataccgat tggcagcgga ccgttgtaga tagagtggct gaggaacccg tctgaacgca
      901 ccgttccggg ggtcagacct acgctccact taccgcggat ttgagggaag ccttggtcaa
      961 ctgcggagta gttccagttg ccaccgacaa ccagtgggta ctctccggag gcaaaggtgt
     1021 tcatcggtac aggtgcctga ggaatcttgt aaactgtgaa gagctccatc agttccttga
     1081 agccggcgat ggcttcgggt gtgtttagac cactggagac gccgtcgtcg ttgacgtaaa
     1141 cccctccgtg ctgccaaatg aaggtcatga ccgactggaa gtccggaagc aggtactggc
     1201 tgatacaagc aaggttcatc ccgttcgctt ggaactttgg caggagagct ctgacttcgt
     1261 cccaggttgt aggatactcc cacccgtttt cggcgaaaat atcagttctg taataggtaa
     1321 gaccaaatcc gaactctaca ggtatgccaa aagccgttcc cataaactcc aaagaccgca
     1381 tttgcccggg gtagagtttt ttctctatct cggcaaactc ttcgggatgt tcggctctca
     1441 ggtccaccaa acctccgcgc atgccgaact ctaccggcca ctccgaacca aatgaagcaa
     1501 gatctggagt gtcgcctgct gccaaggcca gcatggcttt gctccaaaga tcgtcgtagt
     1561 tcatagccgt aaagtttagt tcaattccgg tttgaggcgt aaagtactcg ttggccatat
     1621 ccttgatcac ttgagcaatc tcgttgggtc tggtccacca gacctccagt gtgactgcgt
     1681 taacccctaa agcaactgct aatattacga gtagaatagc aaagcgcttt ttcatgcttc
     1741 atccccctag ttttgagtat cactgtatat ttcgagaagt cccgcttatt acctgcatta
     1801 gcgttttttt gcactgctct ttgtttcacc tggaacaaga cagtttttcc cagttccgat
     1861 aaggtcctct cttttggctt tcctgagagc tttccgaact agcggccagt ttcggcgatc
     1921 ctgaaactgt agaagagctc tctgcatctt cttctcctct tcatccggaa tgtgtacttt
     1981 ttcccctgta aatggattct ctccagtgta ccacatggtc ccggacacag tgaggggaag
     2041 agggataaaa tcttggacct gttcgaagaa ccggccctgt tttttgatat acagtgcaag
     2101 ttcgatggcg tcgttcagcg tgcttcctgg atgggatgag atcagatagg gaatgacgta
     2161 aggttctttg ccaagatcga gggagatttg gctgtacttc ctgcagaact cctggtaaac
     2221 agggtgagcc ggtttgttca tctggcgcag tactctgctg gagacgtgtt cgggggcaac
     2281 ttttagctgt cccgacacgt ggtttctgat gagttctttc atgaagcctg catccttcaa
     2341 agctgcgtca aaccgcacac cggattgaac aaacactctc tttacgctcg gtacctttgc
     2401 aatattctgc aggagcttta catacgattg ttgatcgatt tccaagttgg ggcagatctt
     2461 tggcgtcata caggacctgt gcttgcagac acctcttctt gcctgcagct tgcaggttgt
     2521 tccgtagaga ttggcagaag gtcctccggc atcgctgatg gttccccgaa actccggatc
     2581 gctggcgatg gtttctgcct cctgaagaat ggacttttcg ctcctggact ggaccactct
     2641 tccctgatgc agggccagcg aacagaagta gcattctcct ggacaccctc tgtgcgttgt
     2701 gatgctgaac tgagtggtct ttaccgccgg cactcctcct tggtctgcgt atgaagggtg
     2761 ggctctcctt tgaaacggta gcccgtagat gtagtccaac tcttcagcag tcatgggctt
     2821 ttccggagga ttaacaaccg cataccagcc gtcctgctgc tcctggacta cgactcgtcc
     2881 ttcgaacggg tcgagctcgt tgtaaaacaa cttgtaagcc ctgccaaact cttttttgtc
     2941 ttgaacagct gcaaaagacg gcagtaccac agtgtctttg gagatgtatg ggtctttttc
     3001 tttgtgtacg gtaccgcgca agccaaaaca tgcttcaaca cctagaccca taaggaagtt
     3061 ctctgcgata gcggcaatct gcttctctcc catcccgtaa acgaggagat ctgctttggc
     3121 atcgaagaga atagacctgc ggacggagtt ttgccagtag tcgtagtgag caatacgccg
     3181 catactagct tcaatgccgc ctagcacaat aggggtatcg gggaagagct ccttcagctt
     3241 gttgctgtag acgattgtcg ctcggtcggg ccgaaggccc gcttttctgc ccggagcata
     3301 agcatcgtct cggcgccgct tattggcagc ggtgtagttt gccaccatgg aatctaggtt
     3361 gcccgccgtg acgccaaaga acagcttggg tctcccaagc tttagaaaat cttcattctc
     3421 gcgccagttc ggttgcgcaa taacgcctac tttgtagccg agactaacca aataacgtcc
     3481 gagaagtgcg gtgccgaagg aaggatgatc aacatacgca tcacctgtca caagcacgat
     3541 gtcgaggctg tcccagcccc attgttgggc ttctttaaga gtcatcggca aaaagagctc
     3601 gtttttcatt tcatgcctcc tgtgccccgc ctggtgtgct gtagcctttt tcccaggtaa
     3661 ggcataaatt atcctgacga ccggaagacg atctcgacca aggttaactt tttgcagaat
     3721 aaaaaggtaa atctagcact attaagaagt atataataag gtcactcaca cggcgagagc
     3781 ggctttcaga cagcagcttc agccgtaaac agcaaggtct ccagcatatt tcatttaaat
     3841 tttccctgca gtaaaaatcg tttaatcgta atcataagcc agggtttttt tcttgacagt
     3901 atatgaagat tattttaaaa taggcgaagt actccgaagt ccaacgtgag tctccgagta
     3961 caagaaaggg ggatgccttg tgtttgattt tctttataac cgtcggattc ctgatgggtt
     4021 caggataccc aaatggatcc tcaaggaata ctttggtata gaggaagaac agctacaagt
     4081 ggaaaccaac ttgattttgg aaaagttaac gcaacagatc gacgatctct ctgatgacga
     4141 tcactccccc atcgctatct ggctggcgat acagattatg gcagatcaga tttccgagac
     4201 tttaagcgat tcgctggaga tctaccacaa gctggcccaa ggagagctga ccgcaaggga
     4261 agcgatagcc ctgacaaggt taaaacagat tgaacgcgag ttctttgaag aagacgacga
     4321 gtaggcgcgg ccccagatgg ggccgctgtt tttttacctg tggagctcgt taaggtattc
     4381 tttaagttct acagggagtt ctgcgtcaaa ctggcagatt gtaccagtcc ggggatgggg
     4441 aaacgccatg tgacaagcgt gcaggaacgt acacccaagc ccttcgtctc cctcaccgta
     4501 tacctcatct cccacaagtg gatgtcctat tgaactgaag tgcaccctga tctggtgggt
     4561 cctgccgctt agtagaactg cttttactag agtgtgatcg gtgtaccgca tcaggacttc
     4621 gtacttggtg gtggcttttc tgccgtcttc tcccacctct cgcctaacac cttcttttgg
     4681 atcgacaatg cccagatcga tgaccccagc ttcactgtga actcttccgt gaaccaatgc
     4741 caggtaatgc ttttccatct cgccgatctc ttctgacaga atcaccgctg ccagcgggtt
     4801 tttggctgca agaaccaaac ctgacgtctc cttatccaaa cggttcacta agtagctgct
     4861 aggacgttcg cctttctgaa ctaagtggcc cgcaatgtag ttagctaaag tccctgaaac
     4921 gtacctcttc ctaggatgca tgaccatgcc cgcaggcttg ttgactacca aaacgtcgct
     4981 gtcttcgtag accacctcaa tcttggaaaa ctccggagta actttggttt caacggccaa
     5041 atacacctcc accacatccc atgccttcac ctcataggag aagtaagagg tctggctgtt
     5101 tatccgcagt tctccacggt ttttaacatt ccgaatgagg ctggtggaaa agtgataatg
     5161 ccttttaaga acatctttta ccttccatcc tgcctcgctt ggcagtatga cagcgcgaaa
     5221 aaacggtctt tctctttcca aaatccatca ctctttccag atgtctccat atatgcaaag
     5281 acgaaagaac cctttgcaga gcaaagggtt atctggcttt ttcgagagca ttctttgcag
     5341 cagctcggaa cttctcactg gtgccggttg ccccggctac gtcgtccact ttatctggat
     5401 cctggactct aaccagctcc tcctcaagct tgtcgatagc gtcaaacacc aacttataag
     5461 gatagtcttc gtctttagga tcttcctcgt taggtgcttg atactcggtg taatcagcct
     5521 ctttgatctt gccgtcttta atggtcagct cgatgtagcc gtaattgccg cgatcatcgg
     5581 catcgctcct accgacatac ttaccgtcat caaacagcct ttcccctacg tcttccagat
     5641 ccttgcctaa atcttctgtg cggtccttga cgtcctcttt aatgtcctcg ggtttttggt
     5701 cgagtacacc atcatcgact cttgagccaa agtagaccac caaaccgacc actacaactg
     5761 ctataacaag tagccatacc acattgcgat tcaacgagga tccactcctt aaaagaatct
     5821 tctgtggtta tgctaccctt ctgagttgcc tttatacaga cctcgctcca ctgctccgtt
     5881 ccatgccaac atcccaccct ggtagttgaa tacctcggta taaccgtttt ccaggaacag
     5941 attggcagct tccagacttc ttctcccgga cctgcaaacg aaaagaatag ttttgtcttt
     6001 agggaaccga tcaatgtgag cggctacatc gcctaaagga accaatatgg agccggggat
     6061 gtgcccttcg gcatactcgt gcggctcccg cacgtccacg atcaaaaggc tctccttttt
     6121 gcctgccatc agttttttaa cctgacctgt tgtaagattg atatccataa aacagcttgt
     6181 ccaacctttc ttgagagtgc tgcaactctt gcttcaacgt tttgccagaa actgtcagag
     6241 agattgagaa tattggaacc ctcttaacaa cacaatacta acaaggcacg ttaaaaggct
     6301 aaatcactct gcttcttagc ctagctgcca aaaaaggttc gtgagggctc ctcatgaacc
     6361 tttttcccta ttaaaaagca ggacaaacac tcagtttatg gaaagggatt agtgctagct
     6421 tttcttgcca atccagtaga tctgcgaaga ataagctctg ctgggattag tttagaactc
     6481 ttgttctcag ggttgatcag tatgtcgatt gccgcttctg caatttcgta aaacggttgt
     6541 ttgatggtgg taaggggcgg ctccaaatag atcgctgcag gagaatcgtc aaagccaacc
     6601 aaagaaatct cgtccgggat ggatatgttg aggttcttta aagcactgtt tgcagcaagt
     6661 gccaactcaa agccggctgc aaatatggca gtaacccttc tcctccgcaa taccacttcc
     6721 agtttttcct gaatcagctt aagagcccag ataccaatgg cattgatatt gatgaactgg
     6781 tcttcggtgt agggtatccc cagttcggtc attttatctt ttgcaccttt aatacgctgc
     6841 tgagtgttgg cagtttccgg ccaagagtaa aaaacgagga tgtctttgtg gcctagctcg
     6901 ccaagatggg agatcgccat ttgagcgccc aagtagttgt ccgcgtctac gaaaggcact
     6961 cccatattct cccagctggc attgatggca actataggaa tacctgatct gccgattctg
     7021 tcgagacact cctgccagtt gtacagagga ttgacgataa cgaaccgctc tctcatccgg
     7081 tctgctcttg ccacatagtc ttccagttca aaagatgtag acgctgtgat aatggaaaac
     7141 tcgcacttgt gaaaccgtcc cacttccatg agattgcgca gcatcgtgcc catataaacg
     7201 tctcgagcta ggtagtgcac agtctgcagc gtcttttcgt aatcattttg gttgcggatg
     7261 aagtcgccca agagcactat gttgattttc gcaggaactt cacttgccgg gtctctggag
     7321 acaacaaacg ttccaactcc catttctctt tcgagataac cttcctggac cagctcttgg
     7381 atggctcgat tggccgtcat ctggctgacg ttatactgat aggcgatttt ccgttctcca
     7441 ggaagtttct cccctacctg atagttccct gtctttatgt ccttaacgat gccctcttta
     7501 accaataaat acctaggctt gtttcctttt ccgcgcattc tcaggggctt acctcctcca
     7561 cctgttatag aacaatcaat cgtacatcgt gggactccta ctcttttcaa ttatatgtta
     7621 tttacgggaa aattcaaggt aaccgtagat tattggaaaa ttaggttttt atatcaccta
     7681 ttttttcaca taaaacccaa aaacgaagga ggatctgatg tgaaaaaggt tgttgtgttg
     7741 ttgctgtgtt tagtgattag cttttctgct cttgccaagg agaggaccct taccatttgg
     7801 gctgcttggg catccgagtg ggttaggatc tttcaggaga tgaccgaccg ctacttcacc
     7861 cccgaaaccg gtattgaggt agaaatcatg cctctgccct gggacagctt cgagcagaag
     7921 ttcctgcttg cagcagcaac tggcgacgtt cccgatgtag gtctgacagg ggggcttgga
     7981 cctgcagacc tcggcgtccg cggggcagtt gtggacctaa aagctcgatt tggccaagag
     8041 gtcgatgagc tgaccagcca atttttcccc ggactaatgc gttctcttac tttctacgac
     8101 tcggtttttg gcatgccaat gatctacggc agctacaatc tattctaccg cacagacata
     8161 ctagaagaac taggactgga tatccccaac acctgggacg aagtccgcgc catgataccc
     8221 aaactccagg caaatgatac gaacttcgcg gtggcctacg gccaccaaag cactgctgac
     8281 ttctctatgt tcctcttcca gcgaaacgga gactggtata cgcctgatct gaccaaatct
     8341 gcgttggata ctcctgaagc aatcatggcc ttcagagagt ttaccgacct ctttaccaag
     8401 caaaacatac ctaaagagat ccagccctac ttccaacctt ttagaactgg cgacatcgcc
     8461 attcaaatcg gaccgacatt cgactacgct aacctgcaga tcgcagctcc agagctgaag
     8521 ggcaagtggg gaatgtccat ggtaccagga acgccaaatg cagacggcga gctgaataga
     8581 gccgcttaca ttggcaccat gggaatcatg atcttcgaaa agagccgcga cattccagga
     8641 gcttgggagt gggtcaa
//
LOCUS       DAOSDA010000011         8516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_5429_length_8516_cov_3.558445, whole genome shotgun sequence.
ACCESSION   DAOSDA010000011 DAOSDA010000000
VERSION     DAOSDA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5429_length_8516_cov_3.558445"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /gene="ftsH"
                     /locus_tag="PLW82_00580"
     CDS             complement(<1..1735)
                     /gene="ftsH"
                     /locus_tag="PLW82_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOJ45807.1"
                     /translation="MIFAIASLATRTEQPQELAYSDLIDRIENGSIRQFTFIGESYAT
                     GTYVDAKNKEVRFETTLLPGAAATLLDKLTEESIDVKIEPEQTIPWWVSFLPNIFLIL
                     LMVVLWVIFFNQLQGGGNRTLSFGKSRAKMHSESAVKVTFADVAGVDEAKQELAEIVD
                     FLKNPKKYSSMGAKIPKGVLLVGPPGTGKTLLGRAVAGEAGVPFFNISGSDFVEMFVG
                     VGASRVRDMFETAKRHAPCIVFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGF
                     EPNTGIIVLAATNRPDVLDPALLRPGRFDRQVVVDLPDVKGREEILQIYMRNKPLAKD
                     VNAETLAKMTPGFTGADLENLMNEAAILATRKNLREITSVEVEEAVDRVTMGPPKARK
                     IMSEKDRKIFAYHEAGHALIGKLISGGDPVHKVTIVGRGLAGGYTLTLPEEDRQTRTK
                     TDLTNYIMYGLGGRVAEEIVFGDVSTGAQNDLARISKIARRMVTEWGMSEKLGPVVFG
                     KNEEQVFLGRDLGHVRNYSEEIASLIDSEVKGLIDECYRKTKELLSENRETLDRMVEA
                     LLEKETLNREEI"
     gene            complement(1846..3264)
                     /gene="tilS"
                     /locus_tag="PLW82_00585"
     CDS             complement(1846..3264)
                     /gene="tilS"
                     /locus_tag="PLW82_00585"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOJ45808.1"
                     /translation="MLYDAFKKELLQLLAMVESEKARKGIAVAFSGGSDSVALLHLLL
                     RFGKEQQLPIYAVHVEHGIRGQTSLDDADFAKSFCRKLAVPLEVVHIDVPGYVAKNSG
                     VSVEEAARTLRYAALKQKAGDFGVSAICFGHHMDDQVETVLLNILRGTGVAGLRGIPR
                     FRRDGGVCYLRPLLGFSKQKILAYCKDWNLEFAQDETNLEMDYTRNRVRLELIPYLET
                     YNPSVKAALHRLSQAAGETEDFLDEKVSEISSRIIRGEKYIGLDLTDYDKMPLVLQKK
                     VLRSLVKELTGNFPDHAQTVLLEEIAQKREGSSYHQLTGQLVASREYDLLYLATCFES
                     LPEPKFLFSEDENGLYSYKDWHLEVVARDGKPVLGSMPFCETVDGDKLSLPLDVRCRK
                     PGDVFHPLGAKGAKKLKDFFIDLKIPKRVRDRVPLVVDAKGNIIWVVGFRISDLVKVD
                     ENTEKYITLRIVSSCHLDSPVL"
     gene            complement(3460..5751)
                     /locus_tag="PLW82_00590"
     CDS             complement(3460..5751)
                     /locus_tag="PLW82_00590"
                     /inference="COORDINATES: protein motif:HMM:NF039380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ45809.1"
                     /translation="MSVVLTRQSTVYRRLSQAKRVAALPYGLRRGLSFLGLVFLGVVT
                     IKGVYGIALGILAHVYSVSYAARNRMPEARSIMLGGLIGILIDSRSLFPLLYWIALWI
                     VLEIFVLKVKPKYKNIKVMTVMASILYPVLIIIGKKLAFPLVTYALYGTLCAVLLANN
                     YKSLVILQERDRVWDNEEVISLIMSSVIFGSSVCVIEVFGLCVGQIVMWLLVAASGML
                     GGPLWGVVAAALVSFSGSLLEYSHCLIMLGAFTGVISMMKGYAAVLIALTFVLIVMNR
                     NLSAVGPEIIVAEICLPFLLRKMQPLKFSVPSFVPPENTESKALELKARIEKFSETLE
                     ELSKVFRGIPIEKAEEGDKTISSLIEAIVAKVCRSCIQRGSCWERDFYTTYANMFDLL
                     VESEESPSGESICEKARRWCSQPKEITDSTLSLLKMRGLDIVWQKRLIESKGVVANQL
                     KGLASVVNNLSNQLELLEKDKHCAEVSSFELKRKGIKFDSIECCRFEHGVTVAISVMK
                     QDGNCLDKEVRGIISRVTGQDCIIERHVCKDGFCELYLVPRYMYDVNVYIRRKPAAGY
                     AICGDSCEEVSLKKGRHLAVLSDGMGVGPEAAIQSSTVVSFIEKFISLDVPSTETLKL
                     VNSVSYLSCEEDTFATVDLVLIDKYTGEVRMSKLGSAQSYVKRGRRVEAITSNSVPVG
                     MLSSIKIVEKHCQFRPKDILVVVSDGVINEGAKRYEDPRWLEKLLINVDGSADEIGDE
                     IFSKLPMDNRDDLTVMVLELKLI"
     gene            complement(5941..6660)
                     /locus_tag="PLW82_00595"
     CDS             complement(5941..6660)
                     /locus_tag="PLW82_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ45810.1"
                     /translation="MRYRARSLFLVVLVFVLVATGILAEMSVSEADELILTKDVANIK
                     KAIEALEKIIAEDPKNAEAHITIAKAHLYLGDRVQESKLEIFEAGKSYAEKATELDPK
                     SPDAYYWLAALTGRVGQTRGILQSLFMINPMKEALDTAIEIDPNYAAAYYVLSLLYTE
                     APGWPVSIGNKQKALEYAQKAVDLAPDDIEFNVHLAKALINNKRTKEAVAVLNRVLEM
                     PHIDTEPEYKEEARELLQKHK"
     gene            7093..7803
                     /locus_tag="PLW82_00600"
     CDS             7093..7803
                     /locus_tag="PLW82_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ45811.1"
                     /translation="MKPNIIPLTISMPFGDSENIVHPVLLWDNESLILVDCGFRGSLP
                     LIEKELEKHELKVTQITGLVLTHHDHDHMGAARALKNINPDLLVYASEKEAPYISGQE
                     KPLRLRQAEELQEKLPPEKQAFGKAFCELLRSIEPVPVDIVLSDNEMLRWCGGCQVLL
                     TPGHTPGHLSLYMTEANAVLTGDAFALENNRPVIANPQFTLDMEQASKSMEKLLALDA
                     DTHYCYHGGVYSSVEGSR"
     gene            complement(8263..8338)
                     /locus_tag="PLW82_00605"
     tRNA            complement(8263..8338)
                     /locus_tag="PLW82_00605"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8303..8305),aa:Lys,seq:ctt)
     gene            complement(8340..8416)
                     /locus_tag="PLW82_00610"
     tRNA            complement(8340..8416)
                     /locus_tag="PLW82_00610"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8380..8382),aa:Met,seq:cat)
BASE COUNT         2249 a         2207 c         1770 g         2290 t
ORIGIN      
        1 caatctcttc cctgttgagg gtctccttct cgagaagagc ttccaccatg cggtcgaggg
       61 tctcgcgatt ctctgacaag agctctttag tctttctata gcactcgtcg attaaaccct
      121 tgacctcaga atcgatgagc gatgcgatct cttctgagta gttccgcaca tgcccaaggt
      181 ctcttcccaa aaagacctgt tcctcatttt tgccaaaaac aactggacca agcttttcac
      241 tcatacccca ttctgtcacc atacggcgtg cgatcttact gattcgtgca aggtcgtttt
      301 gtgcaccagt acttacatcg ccaaacacaa tctcttcagc aactctgcct ccgagaccat
      361 acatgatgta gttggtgaga tctgttttgg tccgtgtttg tctgtcttct tcagggagag
      421 tgagggtgta gccgccggct aatcctcggc ctacaattgt taccttgtga acaggatcac
      481 caccagagat cagtttgccg atcaaagcgt gacccgcttc atgataagca aagatcttgc
      541 ggtctttttc cgacatgatc ttccttgcct ttggaggacc catcgtcact cggtccactg
      601 cttcctcaac ttcaacactg gtaatctctc tgagattctt cctagttgca aggatagcag
      661 cttcattcat gaggttctct aaatcagcac cagtaaatcc aggtgtcatc tttgccagtg
      721 tttcggcatt aacgtctttg gctagtggtt tattccgcat gtagatttgg agaatttctt
      781 cacggccctt tacgtctggg agatcaacaa caacctgtct gtcgaaacgt ccaggtctga
      841 gtaaagcagg atccagtacg tcaggacggt tggtagctgc aagtacgata attcccgtgt
      901 tcggttcaaa accgtccatc tcaacaagca attggttcag agtctgctca cgctcgtcgt
      961 gtccaccgcc gagacctgcg cctctctgac gtccaactgc gtcgatttca tcaatgaaaa
     1021 cgatacaagg agcgtgtctt ttcgcagttt caaacatgtc cctgactcgt gatgcgccaa
     1081 caccaacaaa catctctaca aaatcagaac cgctgatgtt gaaaaacggc acaccggcct
     1141 caccagcaac agcgcgtcct aaaagtgttt taccagttcc gggtggtcct acaaggagaa
     1201 cacctttggg gattttcgcc cccatgctcg agtatttctt aggatttttc aagaagtcga
     1261 cgatctcagc aagctcctgc ttagcttcgt caactccagc aacgtcagca aaggtgactt
     1321 taaccgcgga ctcgctgtgc atttttgcac ggctcttgcc gaaggaaagt gtcctgttgc
     1381 ctccgccttg cagttgatta aagaaaataa cccacaaaac caccataagc agtattaaga
     1441 atatattagg aagaaaactc acccaccatg gtatggtttg ctcgggctca atttttacat
     1501 cgatgctctc ctcagtcagc ttgtccaaca gcgttgctgc ggcccctgga agcagtgtcg
     1561 tctcaaaacg gacttcctta ttcttagcat cgacgtaggt acctgtagca taagattcgc
     1621 cgataaaagt aaactggcga atcgatccgt tttcaattcg atctatcaga tccgaatacg
     1681 ccaactcctg aggctgctcg gttctcgtcg ctaggcttgc tatagcaaaa atcatgagga
     1741 ctgctataag tacatatatc agtaagcttt taaaaatgcg gttcacaaaa cgccctcctc
     1801 ccagagacag cccatagctc tctaccaggg aattattcag agtaattata gcacagggct
     1861 atctaagtga caacttgaga caatacgtaa agtgatgtat ttttctgtat tttcatcgac
     1921 tttaactaaa tcactaatac gaaaaccaac aacccagatg atgttccctt tcgcatccac
     1981 caccagaggg actctgtccc tcactctttt agggattttc agatcgataa agaaatcttt
     2041 gagcttttta gcccctttgg ctccaagggg atgaaacaca tcgcctggct ttctgcatct
     2101 cacatctagg ggcagtgaaa gcttatcccc atcaacggtt tcgcagaaag gcatgctacc
     2161 caagacgggt ttaccatctc tggccaccac ttctaagtgc caatccttat aagaatatag
     2221 accattttca tcttcgctaa acaaaaactt gggttccgga agggactcga aacacgttgc
     2281 aagatagagc aaatcgtact ctctggaagc aactaactga cctgtaagtt ggtgatagct
     2341 actgccctcg cgcttttgtg caatctcctc cagcagaacc gtttgtgcgt gatccgggaa
     2401 gttacctgta agctccttca ccaagcttct cagaactttt ttttgcaaga caagcggcat
     2461 cttatcatag tctgtcagat ctaggccgat atatttctcg ccgcgtatga tcctagaact
     2521 aatttctgaa accttttcat ccaaaaagtc ctcggtttcg cctgcagctt gactcagcct
     2581 atggagcgcc gctttgaccg atggattgta agtctccagg tagggaatga gctctagtcg
     2641 gacgcggttg cgggtgtaat ccatctctag attggtttcg tcttgggcaa actccaggtt
     2701 ccaatccttg cagtaagcga gaatcttctg ctttgaaaaa cccaagagtg gtctcagata
     2761 gcagacccct ccatcccgcc tgaaccgcgg aattccccgt aaacctgcga ctcccgtccc
     2821 tcggagaata ttaaggagaa cggtttccac ttggtcatcc atgtggtggc cgaagcaaat
     2881 agcggaaaca ccgaagtccc cggccttctg cttaagagct gcgtaccgca gagttctcgc
     2941 tgcctcttcc acagagactc ccgagttttt tgcaacataa cccggaacat caatgtgtac
     3001 gacctccaag gggactgcaa gtttcctaca gaagcttttg gcaaagtccg catcgtccaa
     3061 tgacgtttgg ccccttatac cgtgctctac atgcactgca taaatgggta gttgctgttc
     3121 tttgccaaac cgcaaaagga ggtgcagcaa tgctactgaa tcgctgccgc cggaaaacgc
     3181 aacagcgatg cccttcctcg ctttttccga ttcgaccatt gccaaaagct gcagcagctc
     3241 ttttttgaag gcatcataaa gcataagttc acatcctcac aaaccatcac aggctgcatg
     3301 tgtgaaactg aatgtttgga aaaccaaacg ctaatttaat gataccatga ttctgcagat
     3361 tccttgcact gaatttgact aatttcgctt ttacttgacg actttaatac tgtgctaaag
     3421 gcaaaaaaag ccccagggta gacctgggac tgctctttct cagattaact tcagctctaa
     3481 gaccatcaca gtcagatcgt ctcgattgtc catcggcagc tttgagaaga tttcatcgcc
     3541 tatttcatct gccgatccat cgacatttat caatagcttt tccagccatc taggatcctc
     3601 ataccgcttt gcaccctcat taattactcc atcactgaca acaacaagga tgtccttggg
     3661 acggaactga cagtgctttt ctacaatctt gatcgagctg agcattccaa ccggcacgct
     3721 gttactggtg atcgcttcaa cccgtctccc ccgcttgacg taggattgtg cagatcctag
     3781 ttttgacatc ctcacttctc cggtgtattt gtcaattaag acgagatcaa cagtagcaaa
     3841 tgtgtcctct tcacagctca aatagctcac ggagtttacc agctttaacg tctcagtgct
     3901 tggcacatct agagaaatga acttctcgat aaaggaaact actgtactgg actgaatggc
     3961 agcttccggt cctacaccca taccatcaga caaaaccgcc aggtggcgac ctttctttag
     4021 agagacctct tcacagctat cgccgcagat tgcatagcct gctgctggtt tccgcctaat
     4081 gtaaacgttc acgtcgtaca tgtaccttgg taccagatac agttcgcaaa agccgtcttt
     4141 gcagacgtgt cgttcgatga tacagtcctg ccctgttacc cgggagataa tgcctcgcac
     4201 ctctttgtcc aagcagttac catcctgttt cattacgctt atggcaactg taactccgtg
     4261 ctcaaagcgg cagcactcaa tggaatcaaa cttaatgcct tttcttttca gctcgaagct
     4321 ggaaacttca gcacaatgct tgtctttttc cagaagttcc aactgattgc tgaggttgtt
     4381 aactacacta gcaagtccct tcagctgatt agccactacc cctttgcttt ctatgagccg
     4441 cttctgccag acgatatcca atccccgcat cttaagaagc gatagagtgg agtcggttat
     4501 ctctttaggc tggctgcacc accttcgggc tttttcgcag atgctctcgc cagatggact
     4561 ctcttcagac tcaactagta gatcaaacat gttggcgtag gtcgtataaa aatccctctc
     4621 ccagcaagag cccctttgga tgcaagacct acagaccttc gccacgattg cttctatcaa
     4681 agaagaaatc gttttgtcgc cttcctcggc tttctcaatg ggaatgcccc ggaacacttt
     4741 tgacagttct tccagtgttt cggagaactt ctcgatcctg gctttgagtt ctaaagcctt
     4801 ggattcggtg ttttccggtg ggacgaagga agggactgaa aacttaaggg gttgcatctt
     4861 cctcagtaag aaaggtaaac agatctctgc cacgataatc tctgggccaa cagcgctgag
     4921 attcctgttc ataacgataa gtacaaaggt caaggcgatc agtactgcag cataaccttt
     4981 catcatggag attacaccgg taaacgcgcc tagcatgata agacagtggc tgtattcgag
     5041 gaggctacct gaaaacgaaa ccaatgccgc tgcgactact ccccaaagag ggccccccaa
     5101 catgccgctt gcggcgacta acagccacat cacaatttgc ccaacgcaca agccgaaaac
     5161 ctcgatcacg cagacgcttg acccaaagat caccgatgac atgatcagtg aaataacctc
     5221 ttcgttatcc caaactcgat ctctttcttg caggataacg agactcttgt aattgtttgc
     5281 caataagacg gcacacaacg ttccatagag agcatacgtg accagtggaa aggccagctt
     5341 cttccctatg atgattagta caggatagag gatagacgcc ataaccgtca taacttttat
     5401 gtttttgtat ttaggcttga ccttgagcac aaagatttcc aaaacaatcc aaagagcgat
     5461 ccagtacaag agtggaaaaa gtgaccggga gtcgatcaaa atcccaatta acccaccgag
     5521 cataatactc cttgcctcag gcatgcggtt ccttgctgca taactgacgc tgtagacatg
     5581 ggctagaatc cctaaggcaa ttccgtacac tccctttatg gttacgaccc ccaaaaaaac
     5641 taaccccaag aaactcaacc cacgccgcaa accgtacggt agagctgcca cccgtttagc
     5701 ctgacttagc cttctgtaaa cggtgctctg tcttgtaagc accacactca tccttctgcc
     5761 tccatacacg ttcctccttg gaatactccc ccacttttat actgtatact aattcacctt
     5821 cgaaatttgt cgaaaatctt cgctccaaat gcgaaaagca atccacttgt ggtggattgc
     5881 tcttcaaatc acttaaggct aagaatcatc tatcgctttt tcgttcggag tatcgcttct
     5941 ctacttatgt ttttgcaaaa gctctctcgc ttcttctttg tactccggtt cggtgtcaat
     6001 gtgtggcatc tcaagtaccc tattcagcac tgctaccgct tctttggtcc tcttattgtt
     6061 gatcagtgct ttagccagat gaacattaaa ctcaatatca tctggagcca gatccacagc
     6121 cttttgggcg tactccagag ccttctgctt attgccaata cttaccggcc atcccggagc
     6181 ttcagtgtaa agtaggctca agacataata cgctgcagca tagttcggat caatctctat
     6241 cgcggtgtcc agagcttctt tcatcggatt gatcatgaaa aggctttgta ggatccctcg
     6301 cgtctgcccc actctcccgg ttaaggcagc cagccagtag taggcatcag gagacttcgg
     6361 atccagttca gtcgctttct ctgcgtaact cttgccagct tcaaaaatct ccagtttact
     6421 ttcttgaact ctgtccccta gatacagatg ggctttggcg atggttatgt gggcctctgc
     6481 attcttcggg tcttctgcga ttatcttctc caatgcttcg atggcttttt tgatgtttgc
     6541 cacgtctttg gtaagaatca gctcatcagc ctcagaaacg gacatctctg ccagaatgcc
     6601 tgttgcaaca agcacaaaca ctaatactac taaaaacaag ctgcgtgcac gataacgcat
     6661 tttcaacacc ccaatcccta agattagcag ctactatgag ttgctaataa cagttctcca
     6721 tatttcaggt tgtccctgca aaaaaatcca actttttcct ttcctgccgg gatgttgctc
     6781 cttcctaaaa accgaaagtg cctaagcagg ttttcaagaa agcgaaaaga acaatggaga
     6841 taccgttgcc catgctgttc gttaatgttg tatctcagct gcatgagttg taagcgtatg
     6901 ggtttcgaaa agagggttaa ctaagattgc tattccaatg acggtacggt taagatgcgc
     6961 taggtcgcct acaagtcttc gagcatcaga ctgctccacg ttagaaacct ctgacgctta
     7021 aagagcaact gtcaaataac agtaccatcg gttcaagttt gtctcaagaa cagaatggag
     7081 tgagtgttaa ctatgaagcc gaacatcata ccccttacga tctcgatgcc gtttggagac
     7141 agcgaaaaca ttgtgcaccc agtcctgctc tgggacaatg aatcgcttat ccttgttgac
     7201 tgcggttttc ggggatctct tccgttaata gaaaaagaac tggaaaaaca tgagttaaaa
     7261 gtaactcaga tcacaggtct tgtccttacg catcatgacc atgatcatat gggtgctgca
     7321 agagcattga agaatatcaa tcccgatctt ctggtatacg cctctgaaaa ggaagcgcca
     7381 tatatttccg gacaagaaaa acccttgaga ttgcgtcaag cagaagagtt acaagagaag
     7441 cttcctccgg aaaagcaggc tttcggtaaa gcgttttgcg agctactgcg cagtattgaa
     7501 ccagttcccg ttgacatagt tctttctgat aacgaaatgc tccgctggtg cggaggctgt
     7561 caggttttgc taacgccagg gcatacacca ggccatcttt cgctatacat gacagaggca
     7621 aatgcggttt taactggaga cgcttttgct ctcgagaata acagacctgt tattgcaaat
     7681 ccccagttta ccttagatat ggaacaagca tccaaatcta tggaaaaact actagcttta
     7741 gacgcagaca cccactactg ctatcatggc ggagtttatt ccagcgtcga aggctcacgg
     7801 taacatctga cagatcagct cccggtgatc tggtaatgct ttataccaag ctcttgttac
     7861 ggacttcttt ctgacattcg tgatcctagg tattggaaga tgggtactgt ttgaccttct
     7921 tggtaaacac gattcttatc ggtgggatgt tgacgaaact gtgtttttaa ccatgataaa
     7981 cgaagcaaaa gactcgtttt cccttcagct atgcagtaca ttgaagaatt tgagagtaag
     8041 atgcaaaaag aacaagaatg atgtcgtgta cgaacattaa gtacttttgc cattgcatgg
     8101 acaaaccaca aagacaatcg gcgtgatgag cacataccgc acaaaacctt cgaacctcgc
     8161 agcgctatac ttcaaaatac ggaaaactca catacacccg ctaattttag cttaggcaaa
     8221 ataaaaaaag accgcaaatg cggtcttttc aacttatgta aatggtgcgc caccggggac
     8281 tcgaacccag gacccgctga ttaagagtca gctgctctac caactgagct agtggcgcat
     8341 ggtagcggcg actggaattg aaccagtgac actgcgggta tgagccgcat gctctgacca
     8401 tctgagctac gccgccaaac tgtgctgcta cttgcagccg agacaattac tattataaca
     8461 aaatggacct taccgtcaag tctttttaca aaaaaagaag gcttccagag atggaa
//
LOCUS       DAOSDA010000012         8474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_5453_length_8474_cov_3.515619, whole genome shotgun sequence.
ACCESSION   DAOSDA010000012 DAOSDA010000000
VERSION     DAOSDA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8474
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5453_length_8474_cov_3.515619"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2436)
                     /locus_tag="PLW82_00615"
     CDS             complement(<1..2436)
                     /locus_tag="PLW82_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ45812.1"
                     /translation="MIFPKKSGGTKAIGNMEHCELIMENLQIGQMLAKTGSWTLELGK
                     EGKFFFSDEVCNILECAPETLEHSFDAFFAYVHPDDLEKVNAAFQRALEGKEYEIEYR
                     IVTQSGREKHVLEKTKTLYDKEGKPEKLIGIIQDLTKQKMIELHLAMVNKNMLEAQTI
                     SGIGTWTYDVARNEFYGTEEMFRIFGVDETEFKNDFRSVVELVHPNDQAKIFDALEKH
                     LAGHSCQIEFRIPQTDGTMKYVVGRAKPVFDENGSVAGIIGTVQDITETKNLQHRLEK
                     SQIILSQAQELAHIGSWEYDIAKDRITWSEEARRIYGITAFYNTLQEFLEYVHPEDVS
                     IIEELMQDPPDKPAVIEFRFIRPDGSVRHISEKVKFVYDNDKPVLVYGAIRDVTQRKL
                     LQESLQTKQTELAKISKRHEALVKEATDVFEVIDPDGTITYISPAVEKVLRCKVEDRL
                     GKKIYDFYDFKKAKRLARMVQHSLEHPRQVFTGTVIYTTPDNENIHIEYHMKNLLDDP
                     DIEGIVINFSDITRKVALQRSLLHSSYHCEETGLPNIKHFREYLKVRCRMAKKKQNKF
                     AVLILKVVGLDEIDYLLGDVIKQKLVRDIVYERLSPLLFTEKVFISRYSDDHFAIILK
                     EPDAECFEIMANKLIDLFAPAFEVGKYQLDAAANIGIAVFPDDGDDPETLETMAKIAL
                     HRARQEGKNTFKFYSSDLDIQNYKESVLRNDLHLAVDRNQLLVQFQPIVDLRTDEIIA
                     AEALVRWDHPEWGIVPPGEFIYLAEETGLITDIGSWVLKEVCREYKRLLDKGLPPIKM
                     SINVSGMQFLETDF"
     gene            3337..4419
                     /locus_tag="PLW82_00620"
     CDS             3337..4419
                     /locus_tag="PLW82_00620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOJ45813.1"
                     /translation="MSSSRIKFSLEQSMAISLVRIIATFMIIACHIFQGYDHMLAWWF
                     NVGVQVFLFMSGYLLAQSSYKTKYEYFSKRFLRIMVPYWILLLLVIPVYHFLGVKVNI
                     ARADVPYYFTGLIGLRRLFDVGLDHLWFMALIIICYVASMILHDVKDRLNELREVPFY
                     IVLALIIGFLPYLESIALLPRTYAPWVATFILGFVISYRYKGRIPIVFGIALTAATLY
                     TTGLRIYNENFVRWNRLPRDFVPWSKFLLGSFIFVLLYVIFKRVNFKAMPKLSKVIKV
                     IDSYTYEIFLVHQIVILGSLSLLYLTTSKGVNILIILGITTVGAVVLKWLCNLAYDRL
                     LPLLGRLPIVKRAKEDEMTSQEQASV"
     gene            4750..6123
                     /gene="rsxC"
                     /locus_tag="PLW82_00625"
     CDS             4750..6123
                     /gene="rsxC"
                     /locus_tag="PLW82_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOJ45814.1"
                     /translation="MPKTFRGGVHLEYHKELTSSRPLQEVEASDVVIIPLKMHVGEQC
                     KPLVKKGDRVLVGQKIGDTDAYVGAPVHSSVSGTVQKIDTALLPSGLRGPAIFIENDG
                     LYEVDPSIRPKGLIEDLTPRQITDIIREAGIVGLGGACFPTHVKLCVPEGKHVEYLLV
                     NAAECEPYLTCDHRTMLERTEDLFYGIKAAAKACGADHVIICVEDNKPDAIEIMQNYC
                     DGQCQVVVLETKYPQGGERQLIKAVLNREVPSGTLPYEVGVVVINVGTAVAIAEAIKT
                     GMPLIKRAVTVTGTIVKNPGNFIVRIGTTMDKLRDVVDGTTEPPSRIIYGGPMMGNTI
                     HELDEPVVKGTSGVVVLSRDESPLIGEPDPCISCARCVDHCPAYLMPNILERMVAENK
                     LDKAKKLGLQDCIECGTCSYVCPSKRHLVQWFRVGKMELAAQRRREEDKAKAKETGKA
                     VSTGGGM"
     gene            6125..7036
                     /locus_tag="PLW82_00630"
     CDS             6125..7036
                     /locus_tag="PLW82_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOJ45815.1"
                     /translation="MATSELLTVASSPHIRDKTSVEKIMFSVALALTPAALVGVYVFG
                     WHALWVLLTSIGAAIAAEVIWQKLRGQRLRVYDGSALVTGLLLGMNLPPTAPLWMAAL
                     GSVFAIIIGKQVYGGLGANPFNPALVGRVFLTASFPALMTKWAFPAGYVDAFGQATPL
                     ASSVEVPMSKLFLGTIPGCIGEVSAIALLIGGLYLLIKRYIDYSIVGGILGTVVVLSL
                     IMGVDPLFHLLSGGLLLGAFFMATDYVTSPVTPRGRWIFGIGIGLLVMLIRQFSSMPE
                     GVSFSILLMNAAVPLINRATRPRIYGR"
     gene            7043..8110
                     /locus_tag="PLW82_00635"
     CDS             7043..8110
                     /locus_tag="PLW82_00635"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOJ45816.1"
                     /translation="MKKNSIFKMVVVLVVISVASGLVLTLANSKLGPIIAARESDKMR
                     IVAQELLPEGATITKVTRYTELASLVSSARTAAGLSATWTWDPNAAPVETTVKEPEPA
                     PAVEPVIDMDAWAAFFTAVEEPQEPANPWAEYGIELVSVDNTPKELSKTWEDFGVRFA
                     SVDNTPQELEKEVPVISWADFGLEVATVSTTPEPEVSVPTFSWEDFGLEAPVVVSPTP
                     SASEAPAEEEPEEEAPSFEIYQAVQGGKLVGVAIVSKAQGYGGDVVIMVALDGTLESI
                     LGIRVLQQKETAGLGTLITEPKFTSQFEGKATGNPFEVGVDVDGVSGATVSSRAVTTA
                     IKQGLMNLEQFLKDGTVKEGA"
     gene            8112..>8474
                     /gene="rsxE"
                     /locus_tag="PLW82_00640"
     CDS             8112..>8474
                     /gene="rsxE"
                     /locus_tag="PLW82_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOJ45817.1"
                     /translation="MAKTNYVKEFLKGLWEEHPTFVMLIGLCPVLATSTSLKNALAMG
                     ISTTAVIVLSNILISLVRNFIPDEVRLPSYIIIVATFVTLVDLFLNAYFPSIYQVLGI
                     FIPLIVVNCIVIGRAEAFA"
BASE COUNT         2047 a         1975 c         2125 g         2327 t
ORIGIN      
        1 aaagtctgtt tctaaaaact gcattcccga aacgttgatg gacatcttta tcggcggtag
       61 ccccttatcc aggagacgtt tgtactcgcg gcatacctct ttcaataccc aactgccgat
      121 gtcggtgatg aggccggttt cttcagcaag atagataaac tcccctggag gaactatgcc
      181 ccattccggg tgatcccacc tgaccagcgc ttctgctgca atgatctcat cggtccgcag
      241 atcaactatg ggctggaact gaaccaagag ttgattccta tcaacggcaa ggtggaggtc
      301 gttccggagc accgactcct tgtaattctg aatgtccagg tccgaagaat agaacttgaa
      361 ggtgttcttt ccttcctgac gggctctgtg gagggctatt ttcgccattg tctccagagt
      421 ttctggatcg tctccgtcat cgggaaagac cgctataccg atatttgctg cagcatccag
      481 ctgatattta cctacttcaa acgcaggggc aaagagatcg atgagtttat tggccatgat
      541 ctcgaagcat tcggcatcgg gctctttgag gatgatggca aagtggtcgt cggagtagcg
      601 gctgataaac accttttctg taaatagcag gggagacagc ctttcgtaaa cgatgtctcg
      661 gactagcttc tgtttgataa catcgcctaa cagatagtcg atttcatcca agccgaccac
      721 tttcagtata agcactgcaa acttgttttg cttcttcttg gccatcctgc agcgcacttt
      781 tagatactct cggaaatgct tgatgttcgg cagaccggtt tcctcacagt ggtaggacga
      841 gtggaggagg ctccgctgca gggcaacttt gcgggtaatg tcgctgaagt tgatgacgat
      901 tccctcaatg tcagggtcgt ctaagagatt cttcatgtga tactcgatgt ggatgttctc
      961 gttatcaggt gtagtataaa ttacggttcc ggtaaatacc tgccgtgggt gttccaaact
     1021 gtgctggacc attctagcaa ggcgtttggc ttttttgaag tcgtagaagt cgtaaatctt
     1081 cttgccaagc cggtcttcca ctttgcagcg gaggactttt tccactgcag gactgatata
     1141 cgtaatcgtt ccgtcgggat cgataacttc gaagacgtcg gttgcttctt tgaccagggc
     1201 ttcgtgcctc ttgctgatct tagccagctc cgtctgcttt gtttggagac tttcctgaag
     1261 gagctttctt tgggtaacat cgcggatcgc tccgtagaca agcacgggtt tatcgttgtc
     1321 ataaacaaac ttaactttct cgctgatatg tctcacagat ccatcgggtc taataaacct
     1381 aaactcgata acggccggct tgtctggggg atcctgcatc agttcttcga tgatgctcac
     1441 gtcttcagga tggacatact ctaagaattc ctgcagagtg ttgtagaaag cggtgattcc
     1501 gtagattctt cttgcttctt cagaccaggt tatccgatct ttagcgatgt catactccca
     1561 gctgccgatg tgagctaatt cctgtgcttg ggaaaggatg atctggcttt tttccaatcg
     1621 gtgctggagg tttttggttt ctgtaatgtc ctgaactgtg ccgatgatgc cggcgacact
     1681 accgttttcg tcaaagaccg gcttggctct gccaactaca tacttcatgg tcccatctgt
     1741 ctggggaatg cgaaactcta tctggcagga atgtcctgca aggtgctttt ccagtgcatc
     1801 gaagatcttt gcctgatcat ttgggtgaac caattccacg acgcttctaa agtcgttttt
     1861 aaactccgtc tcgtccacac cgaagatgcg aaacatctct tctgttccgt aaaactcgtt
     1921 tcttgctaca tcataggtcc aggtaccgat tccgctgata gtttgggctt ctagcatgtt
     1981 cttgttgacc atggcaaggt gcagttcgat catcttctgc ttggtgagat cctggatgat
     2041 gccgatgagc ttctccggtt tgccttcctt gtcatagagg gttttggtct tttccaaaac
     2101 gtgtttttcc ctaccgcttt gggtgacaat ccggtactca atctcgtatt ccttgccttc
     2161 tagtgccctt tggaaagcag cgttgacttt ttccagatcg tccggatgca cataagcaaa
     2221 gaaggcatca aatgagtgct ccaacgtttc tggcgcgcat tcgaggatgt tgcacacctc
     2281 gtcggaaaag aagaacttac cttcctttcc aagttctagt gtccaacttc cagtctttgc
     2341 aagcatttgg ccaatctgga gattttccat tattaactcg cagtgctcca tgtttccaat
     2401 tgccttggtg ccgccactct tttttggaaa aatcatgata ccccacctca tgaagtaata
     2461 tgagtgcaga tgcacgagct gcagaagggg gggggagagc ttcgagacca tggtacctac
     2521 tctcggagaa gctgcagcta tgtagagctg tcacgggtat acgttaaaga taattgttac
     2581 taataactta gattcgttcg ggaaatctct cttatagact aaggatttcg acattcacgt
     2641 tgcactgacc tgtattgtta tttattttac cagtcctctt taccgatgga ttctttagtc
     2701 ttttggtaca cagatgtaca gttaatgaga gtacttgatc aacctgttcc actactactc
     2761 agaagtcgct accaacttac atgaaggtct ctgtttctta cgacagatac gccaactacc
     2821 agcgccagaa tacagcggat cgaacagctt taccttctgt acttgaccac tacgctgaaa
     2881 gattgctgtt tgctgagata aatggatctc ttgcccctgt gttaaagaga ctatgcagac
     2941 cgatacgcga taatatcgca gatattgtga gggacactct gccggagcag cttcgccagt
     3001 acactaaggg ttttacagaa acctggattc cattcaatgc tggtgttttc tttttgaacg
     3061 ctatacaact ctggcatgat caagattcct gatgcggaag accggacacc tattgcttgt
     3121 tggatatttg agagggagta aacctaaaag ctgagtctcg acctatggca gtccacttaa
     3181 aggtgggctg ctttcttgtt ctcctgcacg ttctctttcg ctgagtgcca ttcagctgca
     3241 cgaaaaagcg aacttttcat ttcccaacca aacaaggaac tccgctcttc tctgagaaaa
     3301 tagatatgaa agactagggg aaaggggtct agaaagatga gcagcagtag aattaagttt
     3361 tctttagagc agagcatggc catatctttg gtaagaatca tcgcgacttt catgatcatc
     3421 gcttgccata tcttccaggg ctacgaccac atgctcgcct ggtggttcaa tgtgggtgtt
     3481 caggtgtttt tgtttatgtc tgggtatctc cttgcacagt cttcctacaa aaccaagtac
     3541 gagtacttct ccaagaggtt cctcaggatc atggttccct actggatcct gcttcttctg
     3601 gtaattcccg tataccactt tctcggggta aaggtaaaca ttgcaagagc cgatgttcct
     3661 tactacttta cagggctgat tgggctccgc aggttgtttg atgtcggtct cgatcacttg
     3721 tggtttatgg cactgatcat tatctgttat gtagcttcaa tgattttgca tgacgtaaag
     3781 gacaggttga atgagctccg agaagttcct ttttacatcg ttctggcact gataattggc
     3841 tttctgccct accttgaaag catcgccctt ctaccgcgca catacgcgcc ctgggtagca
     3901 acctttattc tgggctttgt catctcctac cgctacaagg gcaggattcc gattgtcttc
     3961 ggcatagccc ttaccgctgc cacactctac actacggggc tgcggatcta caatgagaac
     4021 tttgtccgct ggaaccgact tcccagggat tttgtacctt ggtccaagtt ccttctgggt
     4081 agtttcatct tcgttctgct ttacgtgatc ttcaagcgag ttaacttcaa agccatgccg
     4141 aagttgagta aggtgataaa ggtcattgat tcctacactt acgagatctt tctcgtgcac
     4201 cagatcgtga ttttgggttc gctgtctctt ctgtatttaa cgacaagcaa aggcgtgaat
     4261 attctcatca ttttggggat cacgaccgtc ggtgcggtgg tgctcaagtg gctgtgcaac
     4321 ctggcctatg acagactcct gccgcttctc gggagacttc ccatcgttaa gagagcgaag
     4381 gaggacgaga tgacctcaca ggaacaggcg agtgtataga gactactgct tcctgatagc
     4441 taccggtaga gcgggtagct ttcgggaagt ttttctttgc atttattgag caccgtttct
     4501 atatgaaaac ttgaaagaat gtaaaattga aaattcttag ccttgacaat catttgacaa
     4561 agagcctgtc ctggtgtaaa tttttgagcg tggccgcagg gcttaatcta tggtttaagc
     4621 aggatatact tggcggttag tagaaagaca atactgtatg cacagaagga aataaggtgg
     4681 catgaagtgc cgccgttttt gagtgccgtt tattttttgt cttgtatatt gaattacggg
     4741 ggtgattata tgccaaagac gttcagaggt ggagtccatc tggagtacca taaggagctc
     4801 accagcagca ggccactgca agaggttgaa gcatctgacg tagtgattat tccactcaag
     4861 atgcatgttg gcgaacagtg caaacctctg gtcaaaaaag gcgatcgggt cttagttggg
     4921 cagaagattg gcgacactga tgcgtatgtt ggcgctccag tacacagcag tgtatctggc
     4981 acagtacaga agatcgatac cgcactacta cccagtggtc ttagaggacc ggcaattttc
     5041 attgaaaacg atggattgta cgaggtagat ccatccataa gaccaaaagg acttattgaa
     5101 gacctaacac ctcgccagat tacagacatt atccgcgaag ccggcattgt cgggcttggc
     5161 ggagcatgct tccccacaca tgtgaagctg tgcgttcccg aaggtaagca cgtcgagtac
     5221 cttttggtca acgctgcaga gtgcgagcct tatcttactt gcgaccacag aaccatgctc
     5281 gaacgcacag aggatctgtt ctacggcatc aaggctgcag ctaaagcctg cggcgcagac
     5341 cacgtgatca tctgcgttga agacaacaaa cccgatgcca tagagatcat gcagaattac
     5401 tgcgatggcc agtgtcaggt tgtcgttctc gagaccaagt accctcaggg tggcgagaga
     5461 cagctgatta aagctgtgtt aaatagagaa gtgccttcag gcacgcttcc atacgaagta
     5521 ggggttgttg tgatcaacgt cggtaccgct gtggcaattg ctgaagctat caaaaccggc
     5581 atgcctctta ttaagagagc cgtaaccgtc acaggtacca ttgtcaagaa ccctggaaac
     5641 tttatagtgc gcattgggac aaccatggat aagctgcggg acgttgttga cggaacaaca
     5701 gagccgccaa gccgcataat ttacggtgga ccaatgatgg gtaataccat tcacgaactg
     5761 gacgaaccag tggtcaaggg gacatctggc gtcgtggtac tcagccgcga tgagagtccg
     5821 ttgatcggag agccagatcc ctgcattagc tgtgcaagat gcgtagacca ctgccctgca
     5881 tatctcatgc ctaacattct cgagagaatg gttgctgaaa acaaactgga caaggccaaa
     5941 aagctcggtc tgcaggattg catcgagtgc ggcacctgtt cgtacgtttg tccttccaag
     6001 cgccacttag ttcagtggtt tagagttggt aagatggaac tagctgctca aagaagacgc
     6061 gaggaagata aagcaaaggc aaaggagaca gggaaagcgg taagcacagg gggaggtatg
     6121 tagtatggca accagtgaac ttctaacagt cgcatcgtca ccccatatta gagataaaac
     6181 aagcgttgag aagatcatgt tctctgtcgc gcttgcactt acgccagctg ctctggtcgg
     6241 cgtatacgta ttcggctggc atgcgctgtg ggttcttctc acatcgatag gtgcggccat
     6301 agctgcagaa gtcatttggc agaagctgag aggtcagcgt ctgagagttt acgatggttc
     6361 tgcgctggtt acaggtctgc tcctgggcat gaaccttccg ccaactgctc ccctgtggat
     6421 ggcagccctc ggaagcgttt ttgccatcat catcggcaag caggtgtacg gaggactcgg
     6481 agccaacccg ttcaaccccg cactagtggg cagagtgttc ctcaccgcat cttttccggc
     6541 tttaatgact aagtgggctt tccctgcagg ttatgtggat gcctttggtc aggcaacacc
     6601 acttgcaagc agcgtagaag tcccaatgtc taagctgttt ttgggaacta tcccgggatg
     6661 tatcggggaa gtttcggcga ttgccctgct tatcggtgga ttgtatttgt taattaagag
     6721 atatattgat tacagcattg tcggcgggat tctcggtaca gtagtcgtgc tttccctgat
     6781 tatgggagta gatccgctct tccatcttct tagcggcgga ctgcttcttg gcgcattctt
     6841 tatggccaca gactacgtta ccagtccggt tactccaaga ggaaggtgga tctttggcat
     6901 cggcattggt ctgttggtga tgctgatcag gcagttttcc agcatgccag agggcgtaag
     6961 cttctcaatt cttttgatga acgctgcagt tcctctcatc aaccgtgcca caagaccccg
     7021 gatctacggg aggtgacaag tgatgaaaaa gaacagcata tttaagatgg ttgttgtgct
     7081 ggttgtgatc tccgttgcat ctgggttggt ccttactctt gcaaacagca agctcggtcc
     7141 catcatcgca gctcgggaga gcgacaagat gagaattgtg gcccaagagc tgctgccgga
     7201 gggtgcaacg attaccaaag ttacaagata caccgaactt gcatcgcttg ttagcagtgc
     7261 aagaacagct gcaggtctca gtgctacatg gacatgggat ccaaacgcag cacctgtaga
     7321 gactactgtt aaagagccag aacctgcccc agcggtagag ccggtgattg atatggatgc
     7381 ctgggctgcg ttctttaccg cggtggaaga gccgcaggaa ccagcaaatc cttgggcaga
     7441 gtacggcata gaactagtta gtgtagataa tacccccaaa gagctcagca aaacatggga
     7501 agactttggc gtcaggtttg cttcagtgga taacactcca caagaactgg aaaaagaagt
     7561 tcctgttatc agttgggcag acttcggtct agaggtagct actgtttcca ctacacctga
     7621 acctgaagta agcgttccaa cgttcagctg ggaggacttc ggtttagaag ctcctgttgt
     7681 cgtttcgcca actccatctg ctagtgaggc tcctgcagag gaagagcctg aagaagaagc
     7741 accaagcttt gagatctatc aggcagttca gggtggaaag ctggtgggcg ttgctatcgt
     7801 ttctaaagct caaggttacg gcggagacgt tgtaatcatg gtagctctag acggaacgct
     7861 agagagcatc ctcggtatcc gcgtactgca gcagaaagag actgcaggtc tcggtaccct
     7921 gattacagaa cctaagttca cttctcagtt tgaaggcaag gcaacaggca atccgtttga
     7981 agtcggtgtg gatgtggacg gcgtcagcgg tgctaccgtt tcgtcgagag ctgtgaccac
     8041 agcgatcaag cagggcctga tgaatcttga acagttcctg aaagacggaa cagtaaagga
     8101 gggtgcatga tatggcaaaa accaactacg ttaaagagtt tcttaaagga ctctgggaag
     8161 agcacccaac ttttgtgatg ttaattggtt tgtgtccggt tctagcgaca agtacttcgc
     8221 tgaagaacgc acttgccatg ggcatatcga caactgcggt tatcgtactg tccaacatcc
     8281 tgatatcgct ggtaagaaac ttcatacctg atgaagtgag actgcccagc tatatcatta
     8341 tcgtggcaac cttcgttacg ctggttgacc tattcttgaa cgcctatttc ccttcaatct
     8401 accaggttct cggtatcttc attcctctga tcgttgttaa ctgcatcgtc atcggacgtg
     8461 ctgaagcttt tgct
//
LOCUS       DAOSDA010000013         8447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_5478_length_8447_cov_3.517398, whole genome shotgun sequence.
ACCESSION   DAOSDA010000013 DAOSDA010000000
VERSION     DAOSDA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5478_length_8447_cov_3.517398"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLW82_00645"
     CDS             <1..225
                     /locus_tag="PLW82_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45818.1"
                     /translation="FHVSARKHFDAANCDIFYTREVLVQIAGRAGRTVACPTGDVVFL
                     AKKPNKPMKDAKAEIIALNEMARERGEILC"
     gene            315..611
                     /locus_tag="PLW82_00650"
     CDS             315..611
                     /locus_tag="PLW82_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45819.1"
                     /translation="MDKLPPPKRIARNAFVVFTDEIEIPPDLVPAIAASRFYMLGGKT
                     DWVLQKEDGGLSSGVAWHLRVPLVEEPHPHTLVVDLRWKNARLLDSLQYLFVFI"
     gene            695..2551
                     /locus_tag="PLW82_00655"
     CDS             695..2551
                     /locus_tag="PLW82_00655"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOJ45820.1"
                     /translation="MREQRTDIMEERRFAVSVNWLKPEAEEGANRNGLIYVSDGQMYI
                     LEPLGLGSPAVLVPGQTLRMYVDGVQVNKPISLYEGQQLEVVVPHKEPSRALLLQVSQ
                     NGLEAELRAELEDGFEYGLRDSPHTVRLSLEAELKHTKNPQRFTKKEVLEFLQNRGIT
                     YGLIEQNIDYFVETQDGNPVVVAKGKPPILPQDARIDFKVRFETERILAQEDEVRVDF
                     RQRFFIPAVNEGDLLAEVIPGKPGEPGIKVTGEEIPVKEPREIEVTVGKGAMLSPDSK
                     YVYAAKSGRPICQRYQISVEPIFVVEGDVDLKQGNVAFTGDVLIKGNVEPNFEVSSGG
                     SINIQGTVSKASVRSRADLVIGGNAICSTIIAASSQTPYPELIKLYAQIAHTLRKVVA
                     AVNQVKVASALRNIVQSDGQFVRQVIDLKFGELPLVVKRLQDVFIDPEDDLQETLQEI
                     NQELADTLLGHGPRKIEDVNELNEMVRKLVYITRELEVRSRHTYSNIVLPYAQNCQIE
                     ATGTVNVLRGCYYSNIVAGKGIIFGKESFFRGGSMVVFEGDITAGTIGSPAGAKTEIT
                     IVKKGVLTAREVFFNVQVSINMRRYTFSRSYRDVKLYVNNEGKLEFEGLKIE"
     gene            2569..2985
                     /locus_tag="PLW82_00660"
     CDS             2569..2985
                     /locus_tag="PLW82_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45821.1"
                     /translation="MKVKNCPQCGSLFIDVGFRVCERCLKYENEQFEIVKEYLQKNAG
                     VTVEQISEATGVSRQLILQFIKAGRLSGSITKLAGPQVRCERCDKPILEGRICAQCQS
                     ELTEKLRTSSKKDDNDKIPSKVRFLKRRKEEHERGI"
     gene            3091..3381
                     /gene="flgM"
                     /locus_tag="PLW82_00665"
     CDS             3091..3381
                     /gene="flgM"
                     /locus_tag="PLW82_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOJ45822.1"
                     /translation="MKINNTNIGNAAQLYKAQQEAIEKSNKKAELGEEASKSKKTDEV
                     ILSKEAKELAKSSQSEREEKLARLKAEIQAGTYQPELEDIADSIIKHLFGAR"
     gene            3446..3994
                     /locus_tag="PLW82_00670"
     CDS             3446..3994
                     /locus_tag="PLW82_00670"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOJ45823.1"
                     /translation="MQRSQTELETICGLLQEKQMVVAAQITLHEKLIENAKLKLEAIQ
                     NRDIEKLEELTRWDQAVLEEVAKWEDRLEKVIKALATWYRVDIKDFNLSGLLALLNEE
                     PEEVRAFVPELARENSILKEHVETLKELNEKNTFLLRRSLAVINFSLETILGKKPETG
                     SYDEKGRRSKDTKKHSIIDRQV"
     gene            4017..5423
                     /gene="flgK"
                     /locus_tag="PLW82_00675"
     CDS             4017..5423
                     /gene="flgK"
                     /locus_tag="PLW82_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOJ45824.1"
                     /translation="MGSSFFGLEILRSSLQAQRLGLDVTAHNIANANTPGYSRQVVQM
                     RAATPFPAPGMSAGRVGQLGSGVEVAEVQRMRNKFVETQIQKETQTLGYWETKQRNVW
                     QIELIFNEPSDTGLSNSISRYWEAWQRLAGEAEDMGHRAPVAQSAAELSFAFQQTRNQ
                     LTALQKDIDQSVAIEAQKLNRLAEELARINRQIARVDTTGQAPNDLLDRQDQILREMA
                     QIANITVLEGRQKTVTVQISGSTLVEHDLVNKIDIIEPTSPGDLTKLVWSKGQAPVRI
                     TNGSIAALLETRDEILVSVIGDLDKLARTIIDETNKLHRSGYGLNSSTGLNFFEGTDA
                     MTIKVMDNIMADPSNIAASVDPASPGNGDNALAIANVFTSPILEGNTVTLNGFYDGMI
                     SRLGVMGLEATRNVENQSSLVQHLYGEQDQYSAVSLDEEMANMIKYQHAYSAAARMIS
                     TIDEALEIIISRMGVVGR"
     gene            5431..6330
                     /gene="flgL"
                     /locus_tag="PLW82_00680"
     CDS             5431..6330
                     /gene="flgL"
                     /locus_tag="PLW82_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HOJ45825.1"
                     /translation="MRVTQTSVIDNLINNINNNYKRLSRVQEQLSTGKSILLPSDDPA
                     AVGTVLRTKRSINEAEQFISNAEKGASWLDLADSALAEATSVLHRAKELLVRGANGTN
                     SDAERNIIATEVDELLEHLVSIANSNIGGRHIFAGQKTLEKPFTYDKSTRTITYNGDS
                     KQIMVEIAPGISETISVPGIEIFGKADTPGDDGIFAMLIKASDALKNGDDLHPHIGGF
                     DKAIESLLQRRAFIGTKSQGMSLIKERLEEQKINLNNLLSETYYVDVAKASIELMAAA
                     NVHTMALKVGAKIIQPSLVDFLV"
     gene            6469..6822
                     /locus_tag="PLW82_00685"
                     /pseudo
     CDS             6469..6822
                     /locus_tag="PLW82_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
     gene            6834..8201
                     /gene="fliD"
                     /locus_tag="PLW82_00690"
     CDS             6834..8201
                     /gene="fliD"
                     /locus_tag="PLW82_00690"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOJ45826.1"
                     /translation="MNLLGVGSGLDLTSIIDQMMEIERIPITRYETKRGEYTSMQSLW
                     RELNTRLSALSNSLNGLKLQSDFDKMKVTSADEAVVTATANANVVQGNYKVKVIQLAK
                     AEIAMSEKVDPEDAEELLQGEIRISINGKVVVLEETEEPRTLLDVAKAINKTEDIGVV
                     ATVVDGRLVLEAKNTNEEIKFFEGEEENAFLKQIGLLKEEGEGYKTNILQERQGSIIE
                     LNGITIARDKNVIDDAVEGLKLTLKKETNEAVNLSVAADVDSIIAKVKAFVDEYKKVQ
                     AYFDTHTSFTKGADGESNNTGKLFGDSTISNLKSRLRSMVTGVVEGLPEDSIKMLSQV
                     GIQIDRSGNMTLDESKLREVLTEKPDQVKELFTKPETGLTARLQEFIKGYTTSQGIID
                     GKINYLADRIKDANKSIERLEARLEMRKESLIRQYTALDSALASLYSQGDWLATQLTL
                     MTNQK"
     gene            8226..>8447
                     /locus_tag="PLW82_00695"
     CDS             8226..>8447
                     /locus_tag="PLW82_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HOJ45827.1"
                     /translation="MKAYDPYAIYKKTKIETSSPAELVSLLYDEAIKCCKLASSAIEG
                     KKMDIAHTNLLKVQDILDELSYAVDTEKGG"
BASE COUNT         2593 a         1839 c         2138 g         1877 t
ORIGIN      
        1 ttccatgtca gcgcccggaa gcacttcgac gccgccaact gcgacatctt ctatactaga
       61 gaagtcttgg ttcaaatagc aggtagagcg ggaagaacag ttgcatgtcc caccggagac
      121 gtggtgtttc tggcaaaaaa acctaataaa cccatgaaag acgcgaaagc agagatcatt
      181 gctttgaacg agatggccag ggaaagaggg gaaattctgt gctgaaactg ctcagggaaa
      241 cgctcgccgg ccaaagatgc cttctctgca acaagccgcg ggggcttcgt gccatttcag
      301 tgtgccttcg gtgcttggac aagctgccac cgccgaagcg tattgccaga aatgcctttg
      361 ttgttttcac tgatgaaata gaaatccctc cggaccttgt tccggcgatc gctgccagtc
      421 gtttttacat gcttggggga aaaaccgact gggttttgca gaaggaagac ggaggtttgt
      481 cttctggggt tgcgtggcac ctgcgggtac ctctagttga agaaccccat ccccacaccc
      541 tggtggttga cctccgctgg aaaaacgccc gccttttaga ttcactgcag tacttgtttg
      601 tttttatata aatgcgtctt tagtcccagt tatttcagcc aggaaggtat aggaatttgc
      661 accaagaaat atgtaaatgt cgaggtgagt ggccttgaga gaacagagga cagacatcat
      721 ggaagaacgg cggtttgccg tgagtgttaa ctggcttaag ccagaagccg aagagggagc
      781 aaaccgaaat ggccttattt acgtctcgga tggacagatg tatattttgg agcctcttgg
      841 cttgggcagc cctgctgttc tagtgccagg ccaaactcta cgtatgtatg ttgacggagt
      901 gcaggtaaat aaacctatct ctttatatga aggtcaacag ttggaagttg ttgtacccca
      961 taaagagccc agcagagctt tgctgttgca agtatcacaa aacggacttg aggctgagct
     1021 tagggctgaa ctagaagacg gctttgagta cggtctgaga gatagtccgc acacagtaag
     1081 gctaagtttg gaagccgagc tgaagcacac aaagaacccc cagcgcttca ccaaaaaaga
     1141 agtgctcgag tttctccaaa acaggggtat cacctacggg ttgattgaac agaacataga
     1201 ttatttcgtt gaaacgcaag acggaaatcc cgttgttgta gccaagggga aaccacctat
     1261 tctacctcaa gatgccagaa tcgatttcaa ggtgagattt gagaccgaaa gaattctcgc
     1321 tcaagaagac gaagtcagag tcgatttccg ccagcgcttt ttcatccctg cggtcaatga
     1381 aggcgatttg cttgcagaag taattccggg aaagcccggc gagccaggga ttaaggttac
     1441 cggtgaagag attccagtga aggaacctcg agaaatcgaa gtaactgtag gaaaaggcgc
     1501 aatgctttct cctgattcca aatacgttta cgctgctaag tcaggtcgtc ctatctgtca
     1561 gcggtatcag atttccgttg agccgatctt tgtagtagaa ggcgatgtag accttaagca
     1621 gggaaacgtg gcttttacag gagatgttct gattaaaggc aacgtggaac ctaacttcga
     1681 agttagctca ggtggcagta taaacatcca gggaaccgtt tctaaagcca gtgttcgcag
     1741 tcgcgccgat ctggtgatcg gaggcaatgc catctgcagt acgattattg cagcatccag
     1801 ccaaactccc tacccagagc ttatcaaact ctatgctcag attgcccata cgctgagaaa
     1861 agttgtggct gcggtgaatc aggtaaaagt cgcaagtgcc ctcagaaaca ttgtgcaaag
     1921 cgacgggcag tttgtgcgtc aggtcatcga tttgaagttc ggggagcttc cccttgttgt
     1981 caaaagactg caggatgttt tcattgatcc agaagacgac cttcaggaga cgctgcaaga
     2041 gatcaatcag gaacttgctg acacactcct cggtcatgga ccgagaaaga tcgaggacgt
     2101 gaacgagctg aacgaaatgg tcaggaagct ggtgtatatt accagagaac tggaggtaag
     2161 aagccggcac acctactcca acatcgtgct gccctatgca caaaactgtc agatcgaagc
     2221 tacaggcacg gtaaatgtac tcagagggtg ctactacagc aacatcgtgg caggaaaggg
     2281 tattatattt ggcaaggaaa gcttcttcag aggaggttcg atggtggtct ttgaaggaga
     2341 catcacagct ggaaccatcg gatcacctgc aggggccaag acagaaataa ctatcgtaaa
     2401 aaaaggtgtt ttaactgcta gagaggtatt cttcaacgta caggtctcta ttaacatgag
     2461 gcgctatacc ttttctcgga gttatcgcga cgtaaagctt tacgtcaaca acgaagggaa
     2521 actagagttc gaaggtctaa agatcgaata gacaaggagg ggagggcttt gaaagtcaaa
     2581 aattgcccac agtgcggcag tctctttatt gacgtgggtt ttcgtgtttg tgagcgttgc
     2641 ttaaagtacg aaaacgaaca gttcgagatc gtcaaagagt atctgcaaaa aaacgcagga
     2701 gttaccgtag agcaaatctc ggaagctact ggtgttagcc ggcagctgat tctgcagttc
     2761 atcaaagcag ggagactgtc aggcagcatc acaaagctcg ccgggcctca ggtcaggtgc
     2821 gaacgatgcg ataaaccgat tttggaaggc aggatttgtg cccaatgcca gtcggaactt
     2881 actgaaaagc tcagaacttc ctcgaaaaag gatgataacg acaaaatccc ctcaaaagtg
     2941 cggttcttaa agaggcgaaa ggaagagcat gagagaggta tttaaggata ttcgggtata
     3001 gcgtccaata ctggttgata tcacggtttt atatatagcg caatctcgcg atctacataa
     3061 tgaataagac cgtaaaggtg gagtagacct atgaagatca acaacacgaa tataggtaat
     3121 gctgctcaac tttacaaggc gcagcaggaa gctatagaaa aaagcaataa aaaggctgag
     3181 ctaggtgaag aagcctcaaa gagcaaaaaa accgatgagg ttatcctttc taaagaagca
     3241 aaagaacttg ccaaatccag ccaatccgaa agagaagaaa aactggcaag attaaaagct
     3301 gaaatccagg ctggtactta tcaacccgaa ctagaggata ttgctgacag cataataaaa
     3361 catttgtttg gtgctagata agtagaaaag agaaggttgc cacctagcaa cttttctctt
     3421 tttttggtaa ttggaggttt acctaatgca gagatctcaa actgagctgg agaccatttg
     3481 cggtctgctc caggaaaaac agatggttgt tgcagctcaa atcacactgc acgaaaagtt
     3541 aattgaaaac gcaaaactca agttagaagc gattcagaat agagatatcg agaagcttga
     3601 agaactaaca agatgggacc aggccgtatt agaagaagtg gcaaagtggg aagacagact
     3661 ggaaaaggtt attaaggctt tggccacatg gtacagagtt gacatcaaag attttaacct
     3721 atccggtctt cttgcacttt taaatgaaga gccggaagaa gtgagagctt tcgttcccga
     3781 gctagctagg gaaaactcca ttctcaaaga acacgtagag acccttaaag agctcaacga
     3841 aaaaaacact ttcctgctcc gccgcagctt ggctgtaatc aacttcagcc tggaaaccat
     3901 tttgggcaaa aaacccgaaa caggtagtta tgacgaaaag ggacgtagga gcaaagatac
     3961 aaaaaaacac tccattatag acagacaggt ctaagctagg tttaggggga attaccgtgg
     4021 gatcttcctt ttttggttta gaaattctgc gcagttcgct gcaagcacaa agactgggat
     4081 tggacgtaac agctcataac atcgccaacg ccaacacacc tggctactcc aggcaggtag
     4141 tgcagatgcg tgctgcaact ccttttcctg ctcctggaat gagtgcagga agggttggtc
     4201 agctcggtag tggtgttgaa gttgccgaag ttcaaagaat gcgcaacaag ttcgtcgaaa
     4261 cccagattca aaaagaaacc caaactcttg ggtattggga aaccaaacag cgtaacgttt
     4321 ggcagataga gttgatcttt aacgagcctt cggacacagg tctatccaac agcatcagcc
     4381 gctattggga ggcttggcag agactggcag gagaagctga agatatgggt cacagagccc
     4441 cggttgcgca aagcgcagct gaactgtcat ttgccttcca gcaaacccgc aatcaactga
     4501 cggcgctaca gaaggacatc gatcagagcg tcgccattga agctcagaaa ctcaataggc
     4561 ttgctgaaga gctagctaga atcaacaggc agattgccag ggtagatact acagggcagg
     4621 ctcctaatga cctactagac agacaggacc aaatcctgag agaaatggca cagattgcaa
     4681 acatcactgt actcgaggga cggcagaaga cggtaacggt gcagatcagc ggaagcacct
     4741 tagtagagca cgatcttgta aataagatcg atatcattga gcctacatca ccgggagatc
     4801 tcaccaaact ggtctggtcc aaaggtcagg cacctgtacg aatcacaaac ggcagcattg
     4861 ctgcgctttt ggaaaccagg gacgagatac tagtgtcggt aatcggagat ttagataagc
     4921 tagcacggac catcatcgac gagaccaaca agttgcatcg aagtggctat ggtctgaact
     4981 ccagcaccgg cttgaacttc ttcgaaggaa ccgatgccat gaccatcaag gttatggaca
     5041 acatcatggc cgaccccagc aatattgctg cttcggtgga cccggcatct ccgggaaacg
     5101 gcgacaacgc cctagctatt gccaacgttt tcaccagtcc gatcttagag gggaataccg
     5161 taactttaaa cggtttttac gatggaatga tctccagact cggcgtcatg ggcctggaag
     5221 caacaagaaa cgtagaaaac caatcgtcgt tggttcagca tttgtacgga gaacaggatc
     5281 agtactccgc agtgtcccta gacgaagaga tggcgaacat gatcaagtat cagcacgcct
     5341 atagtgctgc ggcaagaatg atctccacca tcgatgaagc cttagagatc attatttccc
     5401 gcatgggcgt agtagggagg tagaagctag atgagagtaa cacaaacctc agttatcgat
     5461 aatttaataa acaatatcaa caacaactat aaaaggctct cgagagtcca ggaacagctg
     5521 agcacaggca agtccattct tctaccttca gatgatccgg ccgcagtagg tactgtcctt
     5581 agaaccaaac ggtcgattaa tgaagctgaa cagttcatca gcaacgcaga aaaaggcgct
     5641 agctggctgg atctagcaga cagtgcttta gcagaagcta cctctgttct ccacagggcg
     5701 aaagagctct tggtcagagg agcaaacggc acaaattccg atgctgaaag aaacatcatt
     5761 gccacagagg tggacgagct cctcgaacat ctggtgagca tcgctaactc gaacatcggt
     5821 ggacgccaca tttttgcagg tcagaagact ctagaaaaac catttactta cgacaagagc
     5881 actcgtacaa taacctacaa cggcgacagc aaacagatca tggtggagat agctccaggg
     5941 atctctgaaa ccattagcgt accgggtatc gagatcttcg gcaaagctga caccccagga
     6001 gacgacggca tctttgcgat gctgatcaaa gcttcagatg ctttgaaaaa cggcgatgat
     6061 ctgcatcctc acatcggcgg cttcgataaa gctatcgaaa gccttctgca gaggcgggca
     6121 tttatcggca ctaagtctca gggtatgtcc ctaattaaag agcgcctgga agagcagaaa
     6181 atcaacctca ataacctttt gtccgagacc tactatgtag atgtagccaa agctagcatt
     6241 gaactgatgg cagctgcaaa cgtccacact atggctttga aagttggtgc caaaattatt
     6301 cagccttcgc ttgtggactt tctagtgtag gagttaagcc gcttttgctt aaaagcggct
     6361 tgtgtgcctt tattcagtaa attggttaca tatacctatt taaaaacatc ttaatcacac
     6421 gatttattta ttaggcttca gactctaggt gtttcggagg tgacagagat gggaatcgaa
     6481 cttaagagag taagtataga tcacacgctg caaaaatctg tagagaagac ttttgacaag
     6541 aagctggttc ccgaacaaag cgaaaaacct gtggacgctc agaagcaaga agagtccaaa
     6601 cttgatcagt cgattcaggt tgcagacgga ctggacactc tggcagaggc gttcaacaaa
     6661 ggtatcagat tccaggtaca tgaggatctc gacagagtat atatagaaat cgtcgacaga
     6721 tcaacaggcg aagtaataaa gtaaatacct cctgaagata tgttgaagat cgcagccaag
     6781 ttccaagagt ttatcggttt gatttttgat gagaaagtat aggaggtgcc agaatgaatc
     6841 ttctcggcgt aggatcaggt cttgatctca cttccataat tgaccagatg atggagatcg
     6901 aaaggatccc cattacgcgg tatgaaacca aacgaggcga atacacgtcg atgcagagcc
     6961 tgtggaggga actcaacaca agactttctg ctctgagcaa ttccctcaac ggtctaaaac
     7021 tccagtcgga tttcgataag atgaaggtta catcagcaga cgaagctgtg gtaaccgcaa
     7081 cagccaatgc caatgttgtg cagggcaatt acaaggttaa ggtgattcag ctggccaagg
     7141 ctgaaattgc catgtctgaa aaagtcgatc cggaagatgc ggaagagctt cttcaagggg
     7201 agattagaat cagcatcaac ggcaaagtcg ttgtgttgga agagaccgaa gaaccgcgga
     7261 ccctgctgga tgtagccaaa gctatcaaca aaacagaaga catcggcgtt gtagcgacgg
     7321 tagtagacgg cagactggtc cttgaggcca aaaacaccaa cgaagaaatc aagttttttg
     7381 aaggtgaaga ggagaacgct ttcttgaagc agatcggtct cttgaaagaa gagggcgaag
     7441 gctacaagac aaatatcctc caggaacgcc agggttccat aatcgagcta aacggcatta
     7501 ctattgcaag agataagaac gtcatcgacg atgccgtgga aggtctcaag ctgacgctga
     7561 aaaaagagac caatgaagct gtgaatctga gcgttgccgc cgatgtagat tccataattg
     7621 ccaaagtcaa ggcattcgtt gatgaatata agaaagtcca ggcatacttt gatacacata
     7681 cctcgttcac caaaggcgca gacggtgaga gcaataacac cggcaagctc tttggcgaca
     7741 gcaccatcag caatctgaaa tctagattga ggtctatggt gacaggtgtg gtcgaaggtc
     7801 tgcccgaaga cagtataaaa atgctgtctc aggtcggcat tcagatcgac agatctggga
     7861 acatgaccct cgatgaaagc aagctgagag aagtcctgac cgaaaagcca gaccaggtga
     7921 aggaactgtt caccaagccg gaaaccgggc ttacagctag gttgcaggaa ttcatcaagg
     7981 gttacaccac ttcccaggga atcatcgacg gcaagatcaa ctacctggcg gatcgcatta
     8041 aggacgctaa caagagcatt gagcgcttgg aagcaaggtt agaaatgaga aaagaaagcc
     8101 ttatcaggca gtacactgcg ttggattcag cgcttgcaag tctgtattct caaggcgatt
     8161 ggttggccac acagcttacg ctgatgacta accagaaata actgtgcgaa aatgggggat
     8221 tttaagtgaa agcctatgat ccatacgcga tctacaaaaa gacaaaaatc gaaacatcaa
     8281 gccccgcaga acttgtaagc ctcctgtatg acgaagcaat taagtgctgc aaactggcat
     8341 ccagtgccat tgaaggaaag aagatggata ttgcgcacac caatcttctc aaagtccagg
     8401 acattctgga tgagctttcc tatgccgtag acaccgaaaa aggtggc
//
LOCUS       DAOSDA010000014         8166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_5715_length_8166_cov_3.493281, whole genome shotgun sequence.
ACCESSION   DAOSDA010000014 DAOSDA010000000
VERSION     DAOSDA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5715_length_8166_cov_3.493281"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PLW82_00700"
     CDS             complement(<1..688)
                     /locus_tag="PLW82_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOJ45828.1"
                     /translation="MEKNPKDGREQKKLVLVGNPNVGKSVVFNYFTGIYVDVSNFPGT
                     TVEISSGTYKDYLVMDTPGIYGVSSFNDEEKVARDVILEADVIVNVVDGVHLERDLFL
                     TLQLADMGIPMVVAVNMMDEVERQKIEIDIKALEHRLGVPVIPIIAAQKVGMEELAEK
                     LESAKPGKKDKKIGQLISQFKKDNLQDAKILLALEADIEIEGIADKREEIYFERRKQV
                     DELCKQIIKYK"
     gene            complement(660..926)
                     /locus_tag="PLW82_00705"
     CDS             complement(660..926)
                     /locus_tag="PLW82_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOJ45829.1"
                     /translation="MDRDSQLQPGQKAKLQNLPSGLVKVQAIRLGLTPGTEVTCVCQI
                     NKGPVVISKNFQQIAIGQELAKQIEVETTVGKVHSDGKKPERWA"
     gene            1092..1607
                     /locus_tag="PLW82_00710"
     CDS             1092..1607
                     /locus_tag="PLW82_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOJ45830.1"
                     /translation="MVSIVHNIDILTNLVDRFVLPEALKQDPNQLSPSQFKILSYLYT
                     HLNVRPRDLAYALNITSPAATYALQKLTSDGFVEMESSTTDKREKTASLTEKGVEAVE
                     TITTAKERLILGMFEAMNPDDQESLLRGLKEFLSSAGVKFGPKQLCLKCGFKHSPKCV
                     LSQGRGQTMED"
     gene            1669..2868
                     /locus_tag="PLW82_00715"
     CDS             1669..2868
                     /locus_tag="PLW82_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOJ45831.1"
                     /translation="MSELIDNNRKKQEQMLKKIILELHAGKSVDEVKEKFKRLIKAVG
                     PNEIASLENALMQEGISEEEIKSLCDVHLSIFEDSLILPEESPKTEGHPLEVLKQENR
                     KLEEIASELRTILAGDLNQESSAKWQALHRELMKVDDHYQKKEHLLFPYLEKKGVYGP
                     PSVMWMIHDEIRDELKGIAKVHEESPGDLAALNSVKEAALAVIKKIEDMIGKEEKVLF
                     PLVTEKFDANDWEEIKQSMPEFTEGLVEVPQDYKEKEQEAASITAEGIVDLGSGKFTI
                     KELAAFLNSLPLDITFVDKNREVRYFTEGKERIFARPRTVLGRKVEKCHPPESVHIVD
                     RIVNELESGKRESADFWINLKGKMIYIRYLPVRTAYGEFLGVLEITQDITEIQKLTGE
                     KRLLDEE"
     gene            complement(2940..3680)
                     /locus_tag="PLW82_00720"
     CDS             complement(2940..3680)
                     /locus_tag="PLW82_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOJ45832.1"
                     /translation="MAAAFWASVLAGLATGLGALPIVFIKKFSHRLRDVLLAFAAGVM
                     VAASAFNLIVPALNEGSIFSVVSGILFGALILGLFERYTPNIDYEKLFGTGDKVAAKR
                     AMLMIVALALHNLPEGLAVGVGFASEAPGMGFALAIAIGVQNAPEGLVVAASLLDSKL
                     SVWKMLGIATLTGLVEPIAAMLGLALVSYVQAIIPFALAFAAGAMLYVVFREMIPESH
                     GHGYAEASTASFILGVVFMIAVNYIFGV"
     gene            complement(3714..4139)
                     /locus_tag="PLW82_00725"
     CDS             complement(3714..4139)
                     /locus_tag="PLW82_00725"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOJ45833.1"
                     /translation="MLKVGVKAPDFVLDSTKGIVHLANINGKVLLVFYPKDDTPLCTK
                     QLCAARDNLTEFLSLNVNVIGVNYGSLESHQKFSAKYDLDFPLCVDTDKKVAKLYEAA
                     TEEGDIIRTVYIVDEDKNIVYAQQGAPTTAELMEALNSF"
     gene            complement(4142..4723)
                     /locus_tag="PLW82_00730"
     CDS             complement(4142..4723)
                     /locus_tag="PLW82_00730"
                     /inference="COORDINATES: protein motif:HMM:NF018084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1054 family protein"
                     /protein_id="HOJ45834.1"
                     /translation="MSSNDFITTKELEIFVIPTFAERMERIRSEISPKLELLGSSLAD
                     GIAKGKGTLLHFHVAKHARRSVNPPDETWVAFSPSARGYKNYAHFAVGVGYFGAFVKL
                     MAKADAQEKTALADAILDMGSPRKIWLQKGPDVVEVKREDVVEAIEKKSYGLETGKVL
                     ESVPGDSFDDWYHWALAEIDALWPLYQYVRKKV"
     gene            complement(4878..6179)
                     /locus_tag="PLW82_00735"
     CDS             complement(4878..6179)
                     /locus_tag="PLW82_00735"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ45835.1"
                     /translation="MHIYYFMPKELLERELVQAKEEGKDISGISADLDGEEALKAVDE
                     LQNRPVSKDFPFDEPDTLDDIRAKRPHHVAKPALALNDEVLLDKILGAWLGRSSGCWL
                     GKPWEGFGMAKGKDGIKNVLVEANRYPMNDYLDFDYSDEFMQKHQLDPSLKGYKYRDF
                     DGMPEDDDLNYTVIGLDIIKRHGHDFTAVDVANTWMMHLPVFHTYTAERIAYKNFLNL
                     ISPPESGFYRNPYREWIGAQIRADFWGYVNPGNPERAAEYAYRDACISHTKNGIYGEM
                     FVAAAIAAAFVEDSIEAVIEAGLNQIPENCRLAAAIREFMEFAKTEPDFEKGLEYIYE
                     KYGFYHPVHTINNALIVVAGLVYGNLDFSKTISIAVMGGWDTDCNGATAGSILGALLG
                     AKKIPEKWIDPINDTLYTGVQGYNKVKISDLAKETLDLIKK"
     gene            complement(6183..6731)
                     /locus_tag="PLW82_00740"
     CDS             complement(6183..6731)
                     /locus_tag="PLW82_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ45836.1"
                     /translation="MTRDSLFHKIFKNVFGRSEEVFEPRIREFNVKTDLEPILSWQED
                     LFTTNFAGFQMNPYFLKEQRRRLSEAALRPFEHGIFVLEHIPGKIGGFIWCKIYDTNE
                     YGIFGSVEQIYLIPELRGKGFGKKLMEKGEEYFAERGAQSIKLLVTISNSAAVSLYQS
                     LGYKATRWEMQKDVKRTQMNGG"
     gene            complement(6792..8042)
                     /locus_tag="PLW82_00745"
     CDS             complement(6792..8042)
                     /locus_tag="PLW82_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45837.1"
                     /translation="MESLYIQFNTDKECEEFLCQLLGGEKEPLAKKAVNTIKVRSTWY
                     DPSMYKEVLPNRDLSSFLKNKGVHSIKLINSLEGKVGSWIVLNLDNQAAPIKNRVFQL
                     YRGKITTVKSYDEETLVDELAAALEGGELTEADDLLLKLCNQNPWFLKNEENRKLALE
                     LAEALFDKCEFRKALDWYMRLFQEDSSNPELYLGIGSIYMLLNDLESALNYFLSGLDL
                     NPGHVLLCYNACLILDRIGEVEIALEEVEVALEVNPSSALLHKLAGDLYFKGTDDLDK
                     VLEHYKQAVFLISHNEPSDVLIKLLNNYSLALAEAGDPDLAEKMLHESYGLDETREDT
                     LINLSAFYGFYKYEYDLAIEYANKALEVNLDSGKAYHNLGLAYLAKGNWDRACFCLYK
                     ARKLLPKDYTPIYNAISQLHAERP"
BASE COUNT         2175 a         1971 c         1772 g         2248 t
ORIGIN      
        1 atttatactt gatgatttgc ttacataact cgtctacttg ctttctgcgc tcgaaataga
       61 tctcctcgcg tttgtccgcg atgccctcta tctcaatatc agcttctaat gcaagtagaa
      121 tcttggcatc ttggaggttg tctttcttaa actgactaat gagctgtccg atctttttgt
      181 cctttttgcc aggttttgca gactccaact tctctgccag ctcttccatg cccactttct
      241 gcgctgcaat aatcgggatc acaggcacgc ctaagcggtg ctctagagcc ttgatatcga
      301 tctcaatttt ttggcgttca acttcatcca tcatgttgac tgcaactacc atgggtattc
      361 ccatgtcagc cagctgtaac gtcagaaaca gatctcgctc cagatgcacg ccatccacaa
      421 cgttgacgat aacatctgct tctaaaataa catccctggc aactttctct tcatcgttaa
      481 acgaagaaac gccatagatg ccaggtgtgt ccataacaag atagtcttta taagtacctg
      541 aagagatctc aacagttgtc ccagggaagt tggaaacgtc tacgtatatg ccggtgaaat
      601 agttgaaaac aaccgactta cctacattgg ggttgccaac aagtactaac tttttctgct
      661 cacgcccatc tttcgggttt ttttccatct gagtgcacct tcccgactgt tgtttctact
      721 tcaatctgtt ttgctagctc ttggccgatt gcaatctgct ggaaattctt actgataaca
      781 actggtcctt tgttgatctg acagacgcag gtcacttccg taccaggtgt aaggccgagt
      841 ctaatggcct gaactttaac taaaccgctt ggtaggttct gtaatttggc tttctgtcct
      901 ggctgtagtt gagaatctct gtcaataagc gaaaaacagg gataaccaga cctttagtga
      961 gttttcctgc accaaccacg ccgcctgagc ctttgacaag tatttgacaa aaaccctact
     1021 gcacttgcag gtcttgcaaa gaaagcctaa catataatta agtaaattaa ttgttttaag
     1081 gagatggaac aatggtatct atagttcata acatagacat actcaccaat ctcgttgatc
     1141 gctttgtact tcctgaagct ttgaaacaag atcccaacca actgtctcct tcccagttca
     1201 aaatcctctc ctatttatac acccatctca atgtacgtcc tagagacctt gcatatgcac
     1261 ttaatatcac ttcccctgca gcaacttatg ctctacaaaa gctaactagc gacggcttcg
     1321 tcgagatgga atcgtctact acagataaac gcgaaaaaac ggcgtcgcta acagaaaagg
     1381 gcgttgaagc tgtggagacg ataaccactg caaaagaacg actcattctg ggcatgttcg
     1441 aggcaatgaa cccggacgac caagaaagcc ttctccgcgg ccttaaggag tttttgtcca
     1501 gtgcaggagt taagtttgga ccaaagcagc tgtgtttgaa gtgtggcttc aagcactctc
     1561 caaagtgtgt actcagccag ggacgtggtc aaactatgga agactaagtc aacgtcaagt
     1621 gggtatatgg gttgtggtag ctcatttatc ttagtgaggg gtgtgaacat gagcgagtta
     1681 atcgataaca accgaaaaaa acaggaacag atgcttaaaa agatcattct cgaacttcat
     1741 gccggtaaaa gcgtggatga agtaaaagaa aagtttaagc ggttgattaa agctgtgggg
     1801 cccaatgaaa ttgcttcact ggagaatgcc ttgatgcaag aaggaatcag tgaagaggag
     1861 atcaagagtc tgtgcgacgt tcacctgagt atcttcgagg acagcctgat tcttccagaa
     1921 gaatcgccaa aaacagaagg ccatccgtta gaggtcctga aacaagagaa ccgcaaactt
     1981 gaagagattg cctcggagct acggacaatt ctggctgggg atctgaacca ggaatcttct
     2041 gcaaaatggc aggctctcca cagagaactg atgaaagtcg atgaccatta tcagaagaag
     2101 gaacacctcc tgttccccta cctcgagaag aaaggtgtct acggacctcc aagtgtaatg
     2161 tggatgattc acgacgagat cagggatgag ctcaagggca ttgccaaagt acacgaagaa
     2221 agtcctggcg atttggctgc cctaaacagc gttaaggaag ctgctcttgc ggtaatcaaa
     2281 aagatcgaag acatgatcgg caaagaagaa aaagtactct tccctctggt gacagagaag
     2341 tttgacgcaa acgattggga agagatcaaa cagagcatgc ccgagttcac agaaggtctt
     2401 gtggaagttc cacaagacta caaggagaaa gaacaagaag ctgcctccat caccgcagaa
     2461 ggcattgtgg acctcggaag tgggaagttt acaatcaaag aactggccgc attcttgaac
     2521 tctctgccac tggacattac tttcgtcgac aaaaacagag aagtccgcta ctttaccgaa
     2581 ggcaaagagc gcatttttgc ccgtcctagg actgttttag gtaggaaggt cgagaaatgc
     2641 catccaccgg aaagcgtcca tatcgttgat cgaatcgtta acgaacttga aagtgggaaa
     2701 cgcgagtcag cggatttttg gatcaacctg aagggcaaaa tgatctacat cagatacctg
     2761 cctgtacgta ccgcctacgg ggagttcctc ggagtactcg agatcaccca agacatcact
     2821 gagattcaaa aactcaccgg cgaaaaacgc ttgctggatg aagaataaac ctatgcctaa
     2881 aaagacatcc cactcaggtc ttctttccct gagtgggatg tcttctgttt agtaagggct
     2941 tatactccaa aaatgtagtt aacagcgatc ataaacacta caccaagtat gaacgaagct
     3001 gttgaagctt ctgcgtatcc gtgtccgtga gattcaggaa tcatttccct aaagactacg
     3061 taaagcatag cacctgcagc aaacgcaagt gcaaacggga ttatcgcctg gacgtaggat
     3121 accagggcca gtcccagcat ggctgcgatt ggttctacaa gccccgtgag cgtggctatt
     3181 cccagcatct tccagacaga aagcttggaa tctaagagcg atgctgccac taccaatcct
     3241 tccggtgcat tttgaacgcc aatcgctatg gccagggcga aacccattcc aggtgcctct
     3301 gatgcaaaac ccacacccac tgcaagacct tcaggcaggt tgtgcagtgc cagggcaaca
     3361 atcattagca tcgccctttt tgcagccact ttgtcgcctg taccgaaaag tttctcatag
     3421 tcgatattcg gagtatacct ctcaaaaagt cccaagatca gtgctccgaa tagtattcct
     3481 gaaactacgc tgaaaatcga accttcgtta agtgccggaa cgattaagtt aaaagcacta
     3541 gctgccacca taacccccgc agcaaaagcc aggagaacgt ctctcaatct atgagagaac
     3601 tttttgataa agacaattgg taaagctcct aatcctgttg caagacctgc aagaacactt
     3661 gcccaaaacg ctgctgccat actcccacct ccacctatag gggcgcatct acattagaag
     3721 ctgtttagcg cttccataag ttctgccgtt gtaggcgctc cttgttgtgc ataaacgatg
     3781 tttttgtctt catctactat atatactgta cgaatgatat ctccttcttc tgttgctgct
     3841 tcgtagagtt ttgctacctt cttatcggtg tcgacgcaca gcgggaagtc caagtcgtac
     3901 ttggcagaaa acttctggtg gctttccaga gatccgtagt ttactccaat aacgttaaca
     3961 ttcaagctaa ggaactcagt aaggttgtct cgagccgcac agagctgctt tgtgcaaagt
     4021 ggagtgtcat ccttaggata aaagaccagt aaaacctttc cattaatgtt agcaagatga
     4081 acaataccct tagtgctatc cagcacaaaa tcgggggcct tgactcctac ttttaacatc
     4141 attacacctt ctttcttaca tactggtaaa gaggccaaag tgcgtcaatc tcagctagag
     4201 cccaatggta ccaatcatcg aaactgtctc ctggaacgct ttcaagcacc ttgcccgttt
     4261 ccaaaccgta gctttttttc tcgatggctt ctaccacatc ttcacgtttt acttctacga
     4321 cgtcaggacc cttctgcagc caaatctttc ttggcgaacc catgtccaaa atggcgtctg
     4381 ccagagctgt tttctcctga gcatcggctt tggccatgag tttgacaaac gcaccgaagt
     4441 agccaacacc tactgcaaaa tgggcatagt tcttgtaccc ccttgcagat gggctgaatg
     4501 ccacccaagt ttcatcagga ggattcaccg atcttctggc gtgcttggca acgtggaagt
     4561 gcaacagagt acccttccct ttagcaatac catctgcaag actgctgccg agaagttcaa
     4621 gtttcggcga tatctcagac ctgattctct ccatcctctc tgcaaatgtc ggtatgacga
     4681 atatttctaa ctcctttgtt gtgataaaat cgttggagct caatttcctg cagcctcctt
     4741 ttgatagtaa aagacgtctg tcttatgatg gtaatacccc atattcccgt cttttaacta
     4801 gtcttcccca tcgctcgcta gatgcaaaaa ggagtcgctt aaactgcgtg cagcgcttaa
     4861 gcggctcctc tattttgcta ttttttaatc agatcgagag tctcttttgc caggtccgag
     4921 atcttgacct tattgtaacc ttgaacgccg gtgtaaagag tgtcgttaat cggatcgatc
     4981 catttttctg gaatcttttt ggctccaaga agtgcgccta agatggagcc tgcagttgca
     5041 ccgttgcagt ccgtgtccca tccacccata actgctatgg agatggtttt ggagaagtcc
     5101 aagttgccgt agacaaggcc tgcaaccact atcaatgcgt tattgatggt gtgtactggg
     5161 tgatagaagc cgtatttttc gtagatatac tcgaggccct tttcaaaatc cggctcagtc
     5221 ttggcaaact ccataaattc cctaatggcc gcggccaaac ggcagttttc tggaatctgg
     5281 ttgagccctg cttcaatgac ggcctcaatt gagtcctcta caaatgctgc tgctattgca
     5341 gctgcaacaa acatctctcc ataaataccg tttttggtgt gggaaatgca ggcgtctctg
     5401 taagcgtact cagcagctct ctctggattt cccgggttaa cgtagcccca gaagtccgca
     5461 cgaatctggg caccgatcca ttctctatat ggattgcggt agaaacccga ctctggagga
     5521 gagatcagat tgaggaagtt cttgtaggca attctctctg ctgtataggt atggaataca
     5581 ggcagatgca tcatccaagt gttggcaaca tctactgcag taaagtcatg cccgtgtctt
     5641 ttgatgatat ccaaaccgat gacggtgtag ttgaggtcgt catcttccgg cataccatcg
     5701 aaatctctat acttataacc cttaagtgaa ggatccaact ggtgtttttg cataaactcg
     5761 tcggagtaat cgaaatccaa gtagtcgttc atcggatagc gattggcttc tacaaggaca
     5821 ttctttattc cgtctttgcc cttagccatg ccaaaacctt cccaaggttt tccaagccag
     5881 cagccgctag atcttcccaa ccaagcgcca agaatcttat ccagcaaaac ttcatcgttc
     5941 agggctaggg caggtttcgc tacatggtga ggtctctttg cgcggatgtc atcgagagtg
     6001 tccggttcgt caaagggaaa gtccttcgag accgggcggt tctgcagctc gtccactgcc
     6061 ttcagtgctt cttcgccatc tagatcagct gaaattccag agatgtcttt cccttcttct
     6121 ttggcctgaa ccaattctct ttccaggagt tccttaggca taaaatagta gatgtgcact
     6181 gtttatcctc cgttcatttg ggttctttta acgtctttct gcatttccca tctggtagcc
     6241 ttgtagccga ggctttggta aaggctaacc gcagcggagt tggagatggt aaccaacagt
     6301 tttatgcttt gcgctccgcg ttccgcaaag tattcttctc ccttttccat aagcttcttg
     6361 ccaaatcctt ttccgcgaag ctctggaata aggtaaatct gttcaacaga accaaagata
     6421 ccgtactcgt tggtatcgta tattttgcac caaataaagc cacctatttt tccagggatg
     6481 tgctctagga caaaaatgcc atgttcaaaa ggacggagtg ccgcttcgct aagtcttctt
     6541 ctctgttctt tcaggaagta cggattcatc tgaaaccctg cgaagttggt ggtaaaaagg
     6601 tcttcttgcc aacttaagat cggctccaaa tccgtcttaa cgttgaactc acgtatcctt
     6661 ggttcgaaga cttcttcgct cctcccgaaa acgtttttaa agattttgtg gaacaggctg
     6721 tctcttgtca ttttcgtcgc tagtagagtg cttctagagc ttactccttt cccagctaaa
     6781 gccaaggata attatggtct ttctgcatgt aattgggaaa tggcattgta aataggagtg
     6841 tagtcctttg gcagaagctt tcttgcttta tagaggcaga aacaggctct atcccaattg
     6901 cccttagcca gataggcaag tccaagattg tgataagcct taccagagtc gagattaacc
     6961 tccagggctt tattagcata ctcgatggcc agatcgtact cgtacttata aaaaccataa
     7021 aaggctgata gattaatcaa ggtgtcttcc ctggtctcat cgagaccgta ggattcatgc
     7081 agcatctttt cagcaagatc cgggtctccg gcttctgcca gagcaagact gtagttgttc
     7141 agaagcttga tcaatacatc agacggctcg ttgtgcgaaa tcaaaaacac cgcttgtttg
     7201 tagtgctcta gcactttgtc aaggtcgtcg gtacccttaa aatagagatc tccagcgagc
     7261 ttatggagga gtgcactact tggatttacc tccagtgcca cttccacttc ctctaaagcg
     7321 atctcgactt cgccaatgcg atccaaaatc aaacaggcgt tgtagcacag aagaacgtgc
     7381 ccagggttta aatccaaacc gctgaggaag tagttcagtg cagactccaa gtcatttaag
     7441 agcatatata tcgaacctat acctaggtac agttcgggat tgctagagtc ttcctggaaa
     7501 aggcgcatat accagtccag agctttgcga aactcgcatt tgtcaaaaag agcttcagca
     7561 agctccaaag ccagcttcct gttctcctcg tttttcaaga accagggatt ttggttgcag
     7621 agtttgagca aaaggtcgtc ggcttcggtg agctctccgc cttccagcgc agcagctaac
     7681 tcatctacta aggtttcttc gtcgtaggac ttaaccgtgg tgattttgcc cctatatagt
     7741 tgaaaaaccc tgttcttgat aggagccgcc tggttatcta gattaagcac aatccagctg
     7801 ccgaccttac cttccaggga gtttatcagt ttaatcgaat gcacgccttt attcttaaga
     7861 aaactgctta agtctctatt tggtagaact tctttgtaca tcgacggatc ataccaggtg
     7921 gagcgaactt taatggtgtt tactgctttc tttgcaagag gctctttttc gccgcctaga
     7981 agctgacaaa gaaattcttc gcattcttta tctgtattga actggatata aagactttcc
     8041 aagctttctc acctctatcc attatctaag tatatgcggt ttcccgaaat tagcaagtaa
     8101 attttcccag ccatgtggtt atcttgtgat agtgtcaggg ctgagatgtt ttgggaatat
     8161 gtcatc
//
LOCUS       DAOSDA010000015         7863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_5978_length_7863_cov_3.056609, whole genome shotgun sequence.
ACCESSION   DAOSDA010000015 DAOSDA010000000
VERSION     DAOSDA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5978_length_7863_cov_3.056609"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLW82_00750"
     CDS             complement(<1..473)
                     /locus_tag="PLW82_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ45838.1"
                     /translation="MKVGIISFAHLHADAYADALKQIPYVEFAGIYDEDADRGKAKAE
                     QFGVHHFTSLEEMLATDIGAVIICSANADHCYYTLKAAEAKKHVLVEKPIATNVEDAN
                     KMIDACKKNNVKLQVAFPVRYNPSMIEAKKIVESGKLGRILAIKGTNNGSMPGGW"
     gene            complement(486..1478)
                     /locus_tag="PLW82_00755"
     CDS             complement(486..1478)
                     /locus_tag="PLW82_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012685434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ45839.1"
                     /translation="MKPALKVGLIGGGTMGSTHAEAYRVIENAELVAVADLVPERAQA
                     IASRTGATIRSAAEIIKDPSIDVIDVCVPTPLHRDFVVAAAEAGKHVFCEKPLARTLE
                     EAEEMLEACKKAGVKLGVGHVVRFFPEYIDAYDRVHRGEIGEVATVRTTRGGFPLPTA
                     WKDWYANYEWSGGLVLDMIIHDFDYLRWLLGPVERVFAKSTSGRDFNRLEYALVTLKF
                     KSGAIAHVEGSWVNSGGFHTRFEIAGTKGLMDYDSRKANPINLTVNTTGGMPGVPVPT
                     RPWVESPYTTEIKNFIDALQGKEGKIVDGNEGYESLRIALAALESIETGKAVSL"
     gene            complement(1530..2018)
                     /locus_tag="PLW82_00760"
     CDS             complement(1530..2018)
                     /locus_tag="PLW82_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOJ45840.1"
                     /translation="MELQSLLQSIHNTLLDKGATLAAAESLTGGEISSLLTNTPGSSG
                     YFLCGICAYANSAKEKLLGVSASTLEKHGAVSRECAAEMLGGIIKAAGSDYGISTTGI
                     AGPTGDTPTKPIGLVYIAVGSKDSFVCREFKFTGKRMDVKNQAVYHALDMLNDFLNAG
                     KS"
     gene            complement(2042..2995)
                     /locus_tag="PLW82_00765"
     CDS             complement(2042..2995)
                     /locus_tag="PLW82_00765"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOJ45841.1"
                     /translation="MKQVTKPTRVVIVGTGFVGSSFAYALAQAGLVSEIVLIDIDRKK
                     AEGEAMDINHGIEFVAPVKVWAGDYSDVKDADVIVITAGVNQKPGETRLDLTRTNSRI
                     TKDVCSEIVKYTKDAIIVMTANPVDILTYVTLKETGYPPNQVIGSGTLLDTARFRFLL
                     GQHLGVDPRSVHAYIIGEHGDSELPVWSSATVGGVPINKFCHKHPPDVCQATLNGIFE
                     EVKNAAYEIIERKGATYYAIGWGLMRIVQAIVKDQRSVLTVSTLLDNYLGISDVCLGI
                     PVVVSRSGIERVVELELSEDEYVKLNESANVLKAMQREIGY"
     gene            3153..5456
                     /locus_tag="PLW82_00770"
     CDS             3153..5456
                     /locus_tag="PLW82_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ45842.1"
                     /translation="MRRILGLILLVSVLSLAAFAYEDLRYGVAVLGTNAGNAEEQAAY
                     RKVADAVWDFVDWELISVDVIDGDTLPLEVLDGYKVLIVPSLVGIDEELTAALVAVAE
                     DGGKLIVAADTAEGVPAEFFDHFGFKVSTGGGTKAKFADANGIVEDKFNVNLNSTVSV
                     LPEKDTKVIATFESEEPAVLLSDKALIIAGNGFNAVANNHNLKDLVIEAIYTFGGDRI
                     LSGFSPLGLDEWRSLISEAKTSVRYARSNIRSSENQFRYPSEEIYEKYNKAEKAAELM
                     DYAYDTGNVLRISPYWQIAAKLAEEVIALNQPVRKYEARAVWMDEGTIATAGNPKRLR
                     ETIRTFAEAGFNMLLPEVVSHGAAIFKNDFGIQDPKYWNWEEDPLTVIVDEAHKLGME
                     VHVWTWVFCAGYRHKFGPMLELHPEWAEQDEAGRVFSNWEYGTAWLNPSNPDVVNFLT
                     DLFAYIVTEYDVDGLHLDYIRYTKDDIGHFGLSPDSRAAFKAEHGFDPATVRVNSAEW
                     KLFNQWRENNVTKFVKHMSETVKAINPNLKISAAVGPDPEYARSHILQNWQNWAQNGI
                     LDFIVTMAYTTDNGVLRTNTTKGLENTKNSVYMYPGLGIWVNTPQNVLSQTQLTRQLG
                     TTGVAMFSTIHLLNAPEKLEVLKKGPFREPAVIPHAEPVKALRGLIEEGADLYKLAGR
                     DEEAESLMKLAASIEENPDDLVGLMKRVAGELDVFLNVLNEERTARDLTSVQYESLSS
                     ALLAAWRITQINIYQNTERDWIEPTPR"
     gene            5615..6274
                     /locus_tag="PLW82_00775"
     CDS             5615..6274
                     /locus_tag="PLW82_00775"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HOJ45843.1"
                     /translation="MSFLLVFVLILVPGCFSSTPQAPPPAKVGFWVQNKQDNAVFVEV
                     PEFMYQTRSEGGTVQVSAAGFFPYQAKGEEIAVLTPDPQFWLAAIMWSEARGQSLEGQ
                     AAVGQVVLNRTRDPRFNNTIVDVIFESSRSNSGRSIYQFSPVIDGQIYLAFLDPNFGS
                     FLDLAEKILAGELLNPALQSALGFFNPEKVQAYDPYKKNWVWKQPVIGQIDSHVFFTV
                     P"
     gene            complement(6399..7274)
                     /locus_tag="PLW82_00780"
     CDS             complement(6399..7274)
                     /locus_tag="PLW82_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45844.1"
                     /translation="MWVLGLVFLLCFSGVADANAGLFQKTAKNAVLHIPWDESKTVTW
                     EFNQKDWGTWNVFGVSVNKEPISARSGTDWEYVYLGRPTTTSAWYEWMGGNHQNEILK
                     TLDFIVDGKKVQDGTYKVSESLVIVETTDLVFPADNMIVGSVVRRYTIEVGSPNRMDF
                     AQSTTWHTDMYMDRAYVCMLPIYKRHGRFFKMGNVEDSFVDGIRTDANKGLAPVTETF
                     LYGDMGYGMIAGIKDLSAVDEYKYSKWGAFVWDLSADHVKLYYPRAYEMGPRFVEAGS
                     VWESESYYIVVEVNE"
     gene            complement(7452..7658)
                     /locus_tag="PLW82_00785"
     CDS             complement(7452..7658)
                     /locus_tag="PLW82_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45845.1"
                     /translation="MMHPKRQMLVGGITLFAGWFLLLAMVIELIPNIVWLSVVSYALS
                     LIGFMIGILGVVSLIQIRKRNDRD"
     gene            complement(7660..>7863)
                     /locus_tag="PLW82_00790"
     CDS             complement(7660..>7863)
                     /locus_tag="PLW82_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter ATP-binding
                     protein"
                     /protein_id="HOJ45846.1"
                     /translation="DPDVENLRTFKEVPTGEPPSLMNPPKGCRFHPRCSKKIKGLCDV
                     EIPPEFQVDDEHSVACWLYKDGK"
BASE COUNT         1992 a         2067 c         1786 g         2018 t
ORIGIN      
        1 aaccagccgc cgggcatgct gccgttgttg gtacccttga tagccaggat tctgccgagt
       61 tttccgcttt caacaatctt tttcgcttca atcatcgaag gattgtagcg tactgggaaa
      121 gctacctgca gcttgacgtt gttcttcttg caggcgtcaa tcatcttgtt tgcatcttca
      181 acgttagtag cgataggttt ctctactaga acatgctttt tcgcttctgc agctttaaga
      241 gtgtagtaac aatgatctgc gttagctgaa cagataatga ccgccccgat atcggttgca
      301 agcatctctt ctaaggaagt gaagtggtga acgccgaact gctctgcttt ggcttttcct
      361 ctgtctgcat cctcgtcata aatacctgca aattctacat atgggatctg cttcagtgcg
      421 tcggcatacg cgtcagcatg aagatgagca aaactgataa taccaacttt cattgcctac
      481 acctcctata acgaaactgc tttgccagtc tcgatcgatt caagagcagc taaagcaatt
      541 cttagtgatt cgtagccttc attgccgtcg acaatcttac cctctttacc ttggagggca
      601 tcgatgaagt tcttaatctc agtggtgtat gggctttcta cccatggtct tgttggcaca
      661 ggaactcctg gcataccacc ggtggtgttt actgtgaggt tgatcgggtt ggctttgcgg
      721 ctgtcatagt ccatcaagcc cttggtgcct gcgatttcga atctggtgtg gaatccgccg
      781 ctgttgaccc agctgccctc aacatgagca atagctcctg atttaaactt caaggttacc
      841 agagcgtact ccagacggtt aaagtcacgt ccagatgtgc tcttggcaaa tactctttca
      901 actggtccga gcagccatct caggtagtca aagtcgtgga tgatcatatc cagaaccaga
      961 ccgccgctcc actcgtagtt tgcataccag tctttccatg ctgtaggcaa tgggaaaccg
     1021 cctcttgtag tgcgtacagt agctacctcg ccgatttcac cgcgatgtac cctgtcgtaa
     1081 gcatcgatgt actcagggaa gaagcgtact acgtgtccaa cgccgagctt aacacctgcc
     1141 ttcttgcagg cttcgagcat ctcttctgct tcctccaacg ttcttgccag cggcttctca
     1201 cagaaaacat gcttgccggc ttcagcagca gcaaccacaa aatcgcgatg cagaggagtt
     1261 ggaacacaga cgtcaataac gtcgatgcta ggatccttga taatctccgc tgccgatctg
     1321 atagttgcac ctgttcttga agctatagcc tgagctctct caggaactaa atctgcgact
     1381 gcaaccagtt ctgcgttttc aattacgcga taggcttcgg cgtgagttga acccattgta
     1441 cctccgccga ttaatccgac ttttaatgct ggtttcaaaa acttcacccc cagattttgt
     1501 ttaagctttg ttgttagttc tgtaaaatgt taactttttc ctgcattgag gaaatcgttt
     1561 aacatgtcca gagcatgata aacagcttga ttcttgacat ccatcctttt tccagtgaac
     1621 ttaaattctc ggcagacaaa actatccttg ctacctacgg caatataaac aagccctatg
     1681 ggctttgtcg gtgtgtctcc agttgggcct gcgattcccg tggtgctaat gccgtagtca
     1741 gaacctgcgg ctttaatgat accgccgagc atctctgcgg cgcactccct gctaacagct
     1801 ccgtgttttt ctagagtgct ggcacttacg cctaaaagct tctccttagc agagttggcg
     1861 taggcacaaa taccacagag aaagtaccca gagcttccgg gagtattggt gaggagactg
     1921 ctgatctcgc caccggtcag agattctgcc gcagcaaggg tcgctccttt atcaagaagc
     1981 gtattgtgaa tgctctgcaa gagactctga agttccaaag gtaaacacct tctccgctaa
     2041 tttagtagcc gatttcacgc tgcatggcct taagcacatt agctgactcg ttgagcttga
     2101 cgtactcatc ttcactgagt tcaagctcta ctactctttc gatacccgac ctggaaacca
     2161 ctacagggat gcccaggcag acatcgctga tcccgaggta gttgtccagg agggtggaca
     2221 cggtcaggac cgatctttga tccttgacta tcgcctggac tattcgcatc aggccccagc
     2281 cgatggcgta ataggtcgca cctttgcgtt cgatgatttc atatgccgcg ttcttgactt
     2341 cttcgaaaat cccgtttaga gtcgcctggc agacgtcagg aggatgcttg tgacagaact
     2401 tgttgatggg gactccacca actgttgctg acgaccagac aggcagctcg ctgtcgccgt
     2461 gttctccgat gatgtatgcg tgaacgctgc ggggatcaac tcccaagtgc tgacccaaaa
     2521 gaaagcggaa tcttgccgta tctagcaagg ttcccgaacc aatgacttga ttgggcggat
     2581 agccggtttc ctttaaggtg acgtaggtca ggatatccac cggattggct gtcattacta
     2641 ttatagcatc tttggtatat tttacaattt cagagcagac gtctttcgta atgcggctgt
     2701 tggttctggt tagatccagc ctggtttcgc cgggtttctg attcaccccg gcggtaatca
     2761 cgataacgtc tgcatctttg acatcggagt agtcaccagc ccagaccttt accggagcaa
     2821 caaactcaat accgtgattg atgtccatgg cctcgccttc ggctttcttg cgatcaatat
     2881 ctatgaggac gatctcggaa acaagtcccg cctgtgcaag agcgtaggca aaactcgaac
     2941 cgacaaatcc cgtgcctaca ataacaactc gagtcggttt tgtgacctgt ttcattttag
     3001 ttcacctctt taactatttg taccacttta ctacctcttt taacctgccc tctaaaagaa
     3061 agttaaaata catagatcca tctcctaggc aggtaaaacc aacctgaagt agaaaaagta
     3121 gagatgaaaa caactagcga aaggagatta aaatgagacg aatcttagga ctaattcttc
     3181 ttgtaagcgt actcagcctg gcagcttttg cttacgaaga cctacgctac ggcgtagccg
     3241 ttttaggcac aaacgctgga aacgctgagg aacaagccgc ctacaggaag gttgcagatg
     3301 ccgtctggga cttcgtagat tgggaactta tctccgttga tgtcatcgat ggggacaccc
     3361 tccctcttga agtactagat ggttacaagg ttctcatcgt tccctctctt gtgggaatag
     3421 atgaagagct cacagctgct ttagttgcag ttgccgaaga cggaggaaag ctgattgttg
     3481 ccgcggatac cgctgaaggt gttccagccg agtttttcga ccatttcggt ttcaaagtga
     3541 gcaccggtgg cggaaccaaa gcaaagtttg ccgatgcaaa cggtattgtc gaagacaagt
     3601 tcaatgtcaa tctgaactct acagtttcgg ttctgcctga aaaggacacc aaagtcatag
     3661 caacctttga atctgaagag cctgctgtac tgctttcaga caaggctttg atcatagctg
     3721 gaaacggttt taacgcagtt gcaaacaacc acaatctcaa agacctcgtc attgaagcta
     3781 tttacacctt tggtggcgac cgcattcttt caggattttc accgctcggt ctagatgaat
     3841 ggagaagctt aattagcgaa gcaaaaactt ctgttagata tgcccgctcc aacatcagat
     3901 cgtctgaaaa ccagtttagg tatccatctg aagagattta cgagaagtac aacaaagctg
     3961 aaaaagcagc agaactcatg gactacgctt acgacacagg caacgttttg cgaatctctc
     4021 cttactggca aattgctgct aagcttgcag aggaggtaat agccttgaac caaccagtgc
     4081 gtaagtatga agctagggct gtctggatgg acgaaggcac catcgccacc gccggcaacc
     4141 ccaagagact cagagaaacc attcgcacat ttgccgaggc cggcttcaac atgctccttc
     4201 ctgaagtcgt ctcgcacgga gccgcaatct tcaaaaacga tttcggcatc caagacccca
     4261 agtactggaa ttgggaggaa gatcctctca cagttatcgt cgatgaggca cacaaacttg
     4321 gcatggaagt tcacgtatgg acctgggtgt tctgtgcagg ttacagacac aagttcggtc
     4381 cgatgctcga actccatcca gaatgggcag agcaggatga agcaggccgg gtattctcca
     4441 actgggagta cggaaccgcc tggctcaacc catctaatcc agatgtagta aacttcctca
     4501 ccgatctgtt cgcctacatc gtcaccgagt acgatgttga cggattgcac cttgactaca
     4561 tccgctacac caaggacgac atcggtcatt tcggtctgtc accagacagc agagcagctt
     4621 tcaaggccga acacggtttc gatcctgcta ccgtaagagt gaacagtgcc gagtggaagc
     4681 tcttcaacca gtggcgcgaa aacaacgtca ccaagttcgt taagcacatg tccgaaacgg
     4741 ttaaagccat caatccgaac ctcaaaatct ccgcagctgt cgggcccgac ccagagtacg
     4801 ccagaagcca cattctgcaa aactggcaga actgggctca aaacggcatc ctcgacttca
     4861 tcgtcaccat ggcctacacc accgacaacg gcgttctccg aaccaacacc actaaaggcc
     4921 tggaaaacac caaaaacagc gtgtatatgt acccaggtct cggcatttgg gtaaacactc
     4981 ctcaaaacgt tctcagccaa acccaactga cccgtcaact aggtacaacc ggtgtggcaa
     5041 tgttctcgac catccatctc ctgaacgcac ctgagaaact tgaggttctc aagaaaggcc
     5101 cgttcagaga gcctgcagtt attcctcacg ccgaaccagt taaagctctg agaggtctca
     5161 tcgaagaagg tgctgacctt tacaagctag ctggacggga tgaggaagct gaatctctaa
     5221 tgaagcttgc agcatctatc gaagagaatc cagatgacct agtaggcctc atgaagagag
     5281 tcgccggtga gcttgacgtc ttcctcaacg tattgaacga agaaagaacc gcccgcgacc
     5341 ttaccagtgt tcagtatgag tccctaagca gcgcacttct tgccgcttgg agaataactc
     5401 agattaacat ctatcagaac acagaaagag attggattga gccaacacca agataagtag
     5461 caaactgacg agattcctta actgctgcag agttttctgc agcagttttt tgcacccctt
     5521 tgtagcggcg cgtttttgga aggtcctgcc actttttccc atctcttgga aaacctgact
     5581 gcgctggtta tgattgcagt aggaggtgtt ttctgtgagc tttctactgg tttttgtact
     5641 tatcttagtc cccgggtgtt tttccagcac cccacaagca ccgcctcctg ccaaggttgg
     5701 gttttgggtc cagaataaac aggataacgc ggtgtttgta gaagtgccgg agtttatgta
     5761 ccagactcgg tccgaaggcg gaactgtaca ggtctcagca gcaggctttt tcccctacca
     5821 agcaaaaggt gaagaaatcg cagtactcac accggatcct cagttctggc tggctgccat
     5881 catgtggtct gaagcacgag gacagagtct cgaaggtcag gctgctgttg gacaggttgt
     5941 tttgaaccgc acccgagatc cccgcttcaa caacaccatc gttgatgtaa tcttcgaatc
     6001 gtcaaggtcc aactcaggaa ggagcatcta ccagttttcg ccggtgatcg acgggcagat
     6061 ctatctcgcg tttctcgacc cgaacttcgg atcgtttctt gatcttgccg aaaagattct
     6121 cgcaggcgag ctgctaaacc ctgcacttca aagcgccctc gggtttttca acccggagaa
     6181 agtacaggcc tacgatcctt acaaaaagaa ttgggtttgg aagcaaccgg tgataggtca
     6241 aatcgacagc cacgtcttct tcacagttcc ctaaaggtgc tagcgtctcc ctgctaaact
     6301 ccagagacca tcccccatct ctcgcaggag ttttgcgcaa aatagccgca catagaaaaa
     6361 gggctctgat aatctcagag cccaattttt atcagattct actcgttgac ttcaacaacg
     6421 atgtaatagc tttcgctttc ccaaacagaa cctgcctcta caaacctcgg tcccatctcg
     6481 taagccctag gatagtacag tttgacgtgg tctgctgaca agtcccagac gaatgccccc
     6541 cacttgctgt acttgtactc gtcaacagca ctgaggtctt ttatgcctgc gatcatgccg
     6601 tagcccatgt cgccgtagag gaacgtctca gtaactggtg ccaggccttt gttagcatcg
     6661 gttctgattc catcgacaaa gctgtcttct acgttcccca tcttgaaaaa gcgcccgtgt
     6721 ctcttgtaga tcggcagcat acaaacatag gcccgatcca tgtacatatc cgtgtgccag
     6781 gtagtgctct gtgcaaagtc catcctattg ggactgccaa cctcaatggt gtatctcctt
     6841 accacgctgc cgacaatcat gttatcggcg gggaagacca ggtcggtagt ttccacaatc
     6901 accagacttt cagaaacctt gtaggtgcca tcctgtactt tcttaccgtc tacgataaag
     6961 tccaaagtct tgaggatctc gttttgatgg tttccgccca tccactcata ccaagcgctt
     7021 gtcgtcgtgg gtcggcccaa gtaaacgtac tcccagtcag ttcccgaacg tgcggagatc
     7081 ggttccttat tgaccgacac cccgaacacg ttccatgtac cccagtcctt ctgattgaac
     7141 tcccacgtaa cagtcttgct ctcgtcccaa ggaatatgca aaactgcgtt ctttgcagtc
     7201 ttttggaaca aacctgcgtt ggcatctgcc acgccgctga agcaaaggag aaaaactaat
     7261 cccaaaaccc acatagcaaa tcctcctatc ggaacttgaa ttaagtaagt gaatctttac
     7321 agcgacaact gattctgcac tgtattagac ttctgctact tacgctccat tccttttatc
     7381 ctgcttaaag cttttaaaaa aggctgcttt actcctctta gatggagtaa agcagccaat
     7441 tgcttgcaaa cctagtcgcg atcgtttctc tttctaatct ggatcaggct aacaacgcct
     7501 agaatgccga tcataaaccc gatcagcgac aacgcgtaag aaacgacgct gagccaaacg
     7561 atgtttggta tcaactctat aaccattgca agaagcagga accacccggc aaacagagtg
     7621 ataccgccaa caagcatctg gcgttttgga tgcatcatat tactttccgt ccttgtacag
     7681 ccagcacgct accgaatgct cgtcatcaac ctggaactct ggaggtatct ctacgtcaca
     7741 gagccctttg atcttcttcg agcaacgagg atggaagcgg cagcccttcg gtggattcat
     7801 caaacttgga ggttcaccag ttggaacctc tttgaaggtc ctcaggtttt ccacatcggg
     7861 atc
//
LOCUS       DAOSDA010000016         7708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_6116_length_7708_cov_3.509343, whole genome shotgun sequence.
ACCESSION   DAOSDA010000016 DAOSDA010000000
VERSION     DAOSDA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6116_length_7708_cov_3.509343"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLW82_00795"
     CDS             <1..317
                     /locus_tag="PLW82_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOJ45847.1"
                     /translation="KEDQYINALARKRAEHMLKTGTISHVIPDLGSPWTSLTTYKISY
                     KNASENLQYGARSVVEAHMYLMNSHSHKTNILRKDFNSVGIAVIPNGKGGYLQVQLFA
                     ER"
     gene            complement(427..1341)
                     /locus_tag="PLW82_00800"
     CDS             complement(427..1341)
                     /locus_tag="PLW82_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOJ45848.1"
                     /translation="MKKQACTLAVVQWKLSMEDYSSVEAFAEKLGATMEEIRTKSREN
                     LLVAFPEDLVTFGIFTELKAAGIEEDSIQEALKKYVKKKFVRLLPHRVKMGTWLGAVF
                     ESSKKLMEEYFEVFREAAEKHNAYVISGSGLVADGKDVFNESRGFGPGDAKIIQRKVN
                     LIELEGEKGLGLTPASLAQVGLWSTELGRIGIAICYDGFFAEIREKLDGADIIVQPSA
                     NPGPWTKEQQEDWLNGAYQMVQTATRVVLNPMLTGKALGLEFYGQSSILVRGEERGLG
                     YLNTDPKPHFVQVAKSPDEGDVLIWDLD"
     gene            1507..2016
                     /locus_tag="PLW82_00805"
     CDS             1507..2016
                     /locus_tag="PLW82_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOJ45849.1"
                     /translation="MRRITTIAMFTAVICVMTFVYVPTPWGVPITGQTLAVLVLGLIL
                     SPKDAAISVALYLLLGTVGLPVFSGARSGIGMLLGPTGGYLWSFLIGAVALSWLKQKG
                     RRVLGAIVFTLIVYFLGCLQLKITGDMTFFEAVKLGMLPFIPGDLVKIALALATAKKL
                     EPTIGDSPA"
     gene            complement(2023..3219)
                     /locus_tag="PLW82_00810"
     CDS             complement(2023..3219)
                     /locus_tag="PLW82_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ45850.1"
                     /translation="MHSVLRSTKLLFFSYYLAFGAYGPYLTRYMTKIGLSGKQIGLLQ
                     GLTSLLAFILPPLWGFLTDARYSKRQSFTLALLGMAVGLICYYFSLSFWPMLFATLVF
                     AVFNAPAIPLLDSIALDAVDRAKSYYGNVRMFGSLGYAVSASLMPYLYNHLQNPGSIF
                     PAAVVFLLLALFMFYHMPKEGIAERQGALKSYRSMTRDFGVLIKTPAFLALVLVAFIQ
                     RIGLVPYYNFLTVYLEGLNATNFIVGYAWAVAIVGEMVVLFNSEKLIRRFGEKNLLLF
                     SLIATIIRWIVYAKVQLPWVIFGLQWIHGFSFAAMHAVCVKLVDKLAPKGMQVSAQSL
                     YSAFYFGLGGFIGSLLIGALFDYFGGSGSITVTMSSLFLFSALASVIAFVIGFAFIKV
                     NRQEQS"
     gene            complement(3270..3638)
                     /locus_tag="PLW82_00815"
     CDS             complement(3270..3638)
                     /locus_tag="PLW82_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOJ45851.1"
                     /translation="MTKQRKLILNILRSTKSHPTADWIYQEARKEIPNISLGTVYRNL
                     KILVQAGEILEMDFGSGYSRYDGNPTNHYHFKCRICGRMEDVELETIESLNRIVEESL
                     KAKIENHRLEFYGVCNDCLD"
     gene            complement(3711..4514)
                     /gene="mutM"
                     /locus_tag="PLW82_00820"
     CDS             complement(3711..4514)
                     /gene="mutM"
                     /locus_tag="PLW82_00820"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOJ45852.1"
                     /translation="MPELPEVEVIRRFLNRNIVQEKIDSLDILYDRSVEFEGDFNASV
                     LCGQRICFVDRKGKYLHLGLDRNDLVIHLRMTGQLYFEPEREPKDHIRWVMNFQSGRK
                     LYFRDVRKFGRLVVHPKGAPKFCKNAEIGFEPWEMPLALWFDELKTERAIKAVLLDQH
                     ILAGVGNIYADEALYQSGIHPERKASSLDQHEANNLLMAVRVVMEKGIRFGGTTFRDY
                     LGGDGKKGSMQDHLSVYQKTGSKCRKCSGPISRIKVAGRSSHFCPRCQK"
     gene            complement(4680..5423)
                     /gene="lgt"
                     /locus_tag="PLW82_00825"
     CDS             complement(4680..5423)
                     /gene="lgt"
                     /locus_tag="PLW82_00825"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOJ45853.1"
                     /translation="MHPVLFSIGGLDIYTYGVFVTLGLFVGLLLLEKQAKKRGLDVDL
                     VSRFAFWGIVAAIVGARIGWVVQYWPMYRGNWWAILNVREGGLAFHGGLILTIIVGFY
                     FVLRYKISLAKLSDAAALPLALGYAIGRWGCFFNGCCWGTPTEVPWGVYSRFAEGLRH
                     PVQIYDSLLTLALFFYLIWRSKSTKFGGQLFLETLMGFAIVRFVVEFFRDSVKFNNWL
                     NQTQVLMLGLFIVPLIIQIINLRRVKSRK"
     gene            complement(5439..6374)
                     /gene="trxB"
                     /locus_tag="PLW82_00830"
                     /pseudo
     CDS             complement(5439..6374)
                     /gene="trxB"
                     /locus_tag="PLW82_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458751.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
     gene            complement(6423..7424)
                     /locus_tag="PLW82_00835"
     CDS             complement(6423..7424)
                     /locus_tag="PLW82_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOJ45854.1"
                     /translation="MKRYLTFNKLIVLLFVGLIVSVIVASSLGAVKLPGKWIIGKLLG
                     REVEGPSYWETIFWQIRLPRVVGAVLVGAVLALSGTAFQGVLRNSLADPYILGVSAGA
                     SLGAAIASLLPITSVYGFSFAAFLGALAVTLLVYTISTNGLVRRVQDLVLAGVAVGSV
                     ASSMVSVIMVLNRASMDKIVMWMMGSFSGFTWSNVKMLLFSLVIGGWILHSKSRVLNA
                     LLIGEEAAWNLGINVTRETYIVLTTASFLAASAVSVTGVIGFVGLMVPHIIRLIIGPD
                     HKYLLPASVLGGGLLVVVTDCIARTVIAPTELPVGVITSLLGGPFFLYLLRLNRRNL"
     gene            complement(7399..>7708)
                     /locus_tag="PLW82_00840"
     CDS             complement(7399..>7708)
                     /locus_tag="PLW82_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45855.1"
                     /translation="NDPLMTAGKGTLWDDVITKAGGINVGASALGAFPVFNEEDLIAQ
                     NPDLIILTNFNLEEAMQRPVYASFRDRLLEINPSLYTFPTKRLWDGLEELNEALFNLQ
                     "
BASE COUNT         1986 a         2128 c         1698 g         1896 t
ORIGIN      
        1 tcaaggagga ccagtacatc aacgctcttg ccagaaagag agccgagcac atgctcaaga
       61 cggggactat ttctcacgtc atcccagatt tgggctcacc ttggacaagc cttacgacct
      121 acaagatctc ctacaagaac gccagcgaaa acctgcagta cggagcaaga agcgtagtag
      181 aagctcacat gtaccttatg aacagccaca gccacaaaac caatattctc cgcaaggact
      241 tcaacagcgt gggtatcgcc gttattccta acggcaaagg cggctacctg caggtacagc
      301 tgtttgcaga aagataaacg gaaccgccac agttagtgcg gtctaaaaaa ccaccgtgca
      361 ggtgtaaagc acggtggttt tttctgatct tctctttgct catccccaaa aagcaggcag
      421 acctcgctaa tcgagatccc agatgagcac atctccctca tctggactct tggcaacctg
      481 aacaaagtgg ggcttgggat cggtgttgag gtatccaaga cctctttcct ccccccgcac
      541 cagaatggag ctctggccgt aaaactccaa ccccagtgct ttccctgtga gcatcgggtt
      601 cagcactact ctcgttgcag tctgcaccat ttggtatgca ccgttcagcc aatcttcctg
      661 ctgctcctta gtccagggcc cgggattggc agacggttga acgattatgt ctgcgccatc
      721 tagcttctcc ctgatctccg caaaaaaccc atcatagcag atggcgatgc cgattcgtcc
      781 gagttctgta ctccacaaac cgacctgtgc caaagatgca ggtgtgagtc ccagtccttt
      841 ttcaccttca agctcgatga gattgacttt tctttgaatg atcttcgcat ccccagggcc
      901 gaagcctcgg gactcgttga acacatcttt gccatctgca accagtccac tgccggagat
      961 gacataggcg ttgtgttttt ccgcagcttc cctaaacact tcaaagtact cttccatcag
     1021 cttcttagaa ctctcaaaca ctgctcccag ccacgtgccc atcttcaccc ggtgaggcag
     1081 caggcggacg aacttcttct tcacgtactt tttcagcgct tcttggatgc tgtcctcttc
     1141 aatccctgcc gctttaagtt ccgtaaagat gccaaaggtc acaaggtctt ctggaaacgc
     1201 tacaaggaga ttctccctac tcttggtcct gatctcctcc atcgtggcac cgagcttctc
     1261 tgcaaacgct tccacagaag agtaatcttc catacttaac ttccactgca caactgccag
     1321 cgtacatgcc tgtttcttca tctaaccttc cacccccatt tgaaagactg atcaggcata
     1381 gctctagaga catggaaagc tgcaagcgtt ttaccgcagc agaattgtta gcctgcacat
     1441 tttcctggtt gacaatttcc aggccgactc ctacaattgc acttaaagga ggtttttaac
     1501 ttgtctgtgc ggagaatcac cactatagca atgttcacag cagtaatctg cgtaatgacg
     1561 ttcgtctatg ttcccacacc ctggggcgtg cccatcacag ggcagaccct cgcggtgctg
     1621 gtcttgggac tgattctaag tcccaaagat gcggcaattt ccgtagcgct atatctgctt
     1681 ctcggcactg ttggccttcc tgttttcagc ggcgcgcgga gcggaatcgg aatgctgctc
     1741 ggccccacag ggggttacct ctggagcttc ctcatcggcg ctgtggcctt gagctggctg
     1801 aagcaaaaag gaagaagggt ccttggtgcc attgtcttta cactgatagt ttattttctc
     1861 ggctgcctac agctgaaaat cacaggagac atgaccttct tcgaggcggt caagttggga
     1921 atgctcccct ttattccagg agacctagtg aagatcgcgc tagccctggc aacagccaag
     1981 aagctagaac ccactatcgg agacagccct gcctgaactc cgttagctct gctcctgtcg
     2041 gtttactttg ataaaagcaa aaccaatgac gaaagcaatt acactggcaa gagcggaaaa
     2101 caagaagagt gaactcatag taactgttat acttccagag ccaccgaagt agtcgaaaag
     2161 cgcaccgatc aggagggacc cgataaagcc tccaagaccg aagtagaacg ctgagtagag
     2221 gctctgggcg cttacctgca tgcccttagg tgccagtttg tcgaccagtt tgacgcagac
     2281 agcgtgcatc gcggcaaagc taaagccgtg aatccactgc aagccgaaaa tcacccaggg
     2341 tagctgcacc ttcgcataca caatccagcg aatgatggtc gcaatgaggg aaaacagcag
     2401 caggtttttc tcgccaaagc gcctaatcag cttctctgag ttgaacagga ctaccatctc
     2461 tccgacaata gccactgccc aagcgtagcc cacgatgaag ttcgtagcgt tcagtccttc
     2521 tagatacacc gtcaggaagt tgtagtaggg aacgaggcct attctctgga tgaacgcgac
     2581 cagaaccagc gccaagaaag ctggggtttt gatcaatact ccgaagtccc gagtcatgct
     2641 tctatagctt ttcaaagcac cttgcctttc tgcgatgcct tctttgggca tgtgataaaa
     2701 cataaatagg gcaagtagca aaaaaacaac cgctgcaggg aaaatgcttc ctgggttttg
     2761 caaatggttg tagagatagg gcatcagcga tgcagagacc gcatatccca gtgacccaaa
     2821 catccgcaca ttgccgtagt agcttttggc tctgtctact gcatctagcg caatgctgtc
     2881 gagaaggggt atggccggag cgttgaacac agcaaacacc agagtcgcaa atagcatcgg
     2941 ccagaaactt aagctgaagt agtagcagat aagaccaact gccatcccca atagagctag
     3001 agtaaaagac tgccttttgc tgtatctggc atcagttaaa aaaccccaaa ggggagggag
     3061 aatgaaagcc aaaagggacg ttaaaccctg gagaaggcca atctgctttc ccgaaagacc
     3121 gatcttagtc atgtaacgtg tcaggtaagg gccatatgca ccgaacgcta gatagtagga
     3181 aaagaacaag agcttcgtcg atctcaaaac gctgtgcatg tctacacttc cttatgtaac
     3241 aaagaggcca taggcctctt ttcgtatgtc tagtccaaac agtcgttgca tacaccgtag
     3301 aactctagtc tgtggttttc aattttggcc ttcaaagatt cttcaacaat cctattcaga
     3361 ctttcgatag tctccagttc cacatcttcc atccgtccgc agatgcggca cttgaaatga
     3421 tagtggttgg tgggattgcc gtcgtagcgg ctgtagccgc tgccaaaatc catttccaaa
     3481 atctcaccgg cttgaaccaa gatctttaag tttctatata cagtgcccag gctgatattg
     3541 ggtatctctt ttcgcgcttc ctggtagatc caatctgcgg tggggtgaga ttttgtactg
     3601 cggagtatgt tgagaattaa cttccgctgc tttgtcatgc gtatagactt ttgcttgttc
     3661 actaagactt gcctcctaca cgaaacaatc tccggatgta atcgcgatct ctatttttga
     3721 cacctagggc agaaatgact ggatcgtcct gctactttga ttctgctaat tggtcctgaa
     3781 cactttctgc atttggagcc ggttttttga taaacagaaa gatggtcctg catacttcct
     3841 tttttgccgt ctcctccgag gtaatcccga aaggtggttc cgccaaacct tatacccttt
     3901 tccatgacca ccctaactgc cattaatagg ttattggctt catgttggtc taagctggag
     3961 gcttttcttt cagggtgaat gccgctttga tacagcgctt catcggcata gatgttgccc
     4021 actccggcaa ggatgtgttg atccaagagc acagctttaa tcgctctctc ggtcttcagc
     4081 tcgtcaaacc agagagcaag tggcatctcc catggctcaa aacctatttc agcattttta
     4141 cagaacttgg gcgcgccttt ggggtggacc actaggcgtc cgaacttccg cacgtcacga
     4201 aaataaagct tcctgccgct ttgaaagttc atgacccacc tgatgtgatc ttttggttcc
     4261 cgctccggtt cgaagtacag ctgtcccgtc atacgcaggt ggatcacaag atcgtttcgg
     4321 tccaatccca aatgcaggta cttccccttg cgatcaacaa agcagattct ctgaccgcac
     4381 agaacactgg cattgaagtc gccttcgaac tccactgatc tgtcgtacag tatgtctaaa
     4441 gagtctatct tttcttggac tatattacgg tttaagaacc gcctgattac ttctacttct
     4501 ggtagttctg gcatatcaaa gctgcaaagc tcctactttg tagcagccca cctccgttcc
     4561 catcactact atagcacata tcttttacac gttttatata aacaattttg gagttttcgt
     4621 aaaaactcca aaatagacgc ttatatcttg aaaaaattca tccagtgggt ttggcctttc
     4681 tacttgcggg acttcaccct tctgaggttg attatctgaa taatcaaggg cacgataaac
     4741 aggccgagca tcagtacctg agtctggttc aaccagttgt taaacttcac cgaatcacgg
     4801 aaaaactcca ccacaaagcg gacaatggca aaacccatta gcgtctctag gaaaagctga
     4861 ccgccaaact tggttgactt actccgccag atcagataga agaacagggc aagggtaagc
     4921 agggagtcgt agatctgcac cggatgccta agcccctcgg caaacctgct gtacactccc
     4981 caaggcacct cagtaggtgt tccccaacag cagccgttaa aaaagcatcc ccaccggccg
     5041 atggcatagc caagagccag cggcagtgca gctgcatccg agagttttgc cagggagatc
     5101 ttgtagcgaa gcacaaagta aaatcccacg atgattgtga gtatcaggcc tccgtggaag
     5161 gccaagccac cctctctgac attcaggata gcccaccagt tccctctgta catcggccag
     5221 tactgcacca cccagccaat acgggcgcct acaatcgcag caactattcc ccaaaaggca
     5281 aatctgctta caagatcgac atcgagacct ctttttttcg cctgtttttc taaaagaagc
     5341 agacctacaa aaagtcccaa cgtcacaaag acgccatagg tgtaaatgtc aagtccacct
     5401 atggaaaaca gaactggatg cattttatta ccaccggcct attccttaga ttccaagtag
     5461 tgcagagccg acatggcagc tatagctcca tctgcggtcg ctgtaaccag ctgcctgagc
     5521 acggtatctc ttacgtctcc tgccgcaaac actcctggta tgtttgttct caaatgctcg
     5581 tcagtctcaa tatatccctc cggcgtacgc ttcagcgaat ctgggaagaa ctcgacgttg
     5641 ggtatttgcc caatgtagat gaaaacccca tcagtgttca agacgctttc actgggctcc
     5701 ccttttgcct ctttctcaag attcttgact cttactcccg tcacggagat atcgccaagg
     5761 atctcttcca ctacggtgtt ccatacaaag ctgatcttct cattggcaaa cgctcttcgc
     5821 tgtgcagctg catttgctct cagcttgtcg cgacggtgaa caatagtcac cttcctggca
     5881 aatctggtga ggtataggcc ttcctctact gctgcgtctc ctccaccaac aaccacaaca
     5941 tccttatcct taaagaaggc accgtcgcag gtggcgcagt agcttactcc tctgccgcgg
     6001 aacttgtttt ccccgggaac tcccagcttc cgcggttcag atcaggtgac aagaatcaca
     6061 gttttgccca ggacagtggt gctacccact tcgatcttct tcagttcgcc ctggagatcc
     6121 atggcagtaa tctctccgtt gaggaactct gcccccatgt tctgcgcctg ctcaaacatt
     6181 gccatggcca gatctgggcc ggtcgttcct tgaggaaaac ccgggtagtt ggcaatctca
     6241 aacgttgttg ctacctgccc tccaggcatg cctctttcaa tgatcagggt ctttagggct
     6301 gcacgagcgc cgtatatggc tgctgtcatt cctgcaggtc ccccgcctat gataactaga
     6361 tcgtatgttt ccatatcaac gcctccctct ttattccttt accctctatc tcccgacttc
     6421 aatcacaggt tccttctgtt aagccgtaaa aggtacaaga agaacggtcc cccgagaagg
     6481 gaggttatca cacccaccgg aagctctgta ggtgcaatca cagttcgggc tatacagtcg
     6541 gttacaacaa ccaacaaacc tcctccaagt acactggcag gtagtaggta tttgtgatcc
     6601 ggtccgataa tcagtctgat gatgtgagga accatcaagc ctacaaaacc aataactcca
     6661 gtgacactca ccgctgatgc tgcaagaaac gatgccgttg taagcacaat ataagtctcc
     6721 cttgttacgt taatgccaag attccaagca gcttcttcac cgatcaatag tgcgtttagc
     6781 actctcgatt tggagtgaag tatccagccg ccgatcacaa gtgagaagag aagcatcttt
     6841 acattgctcc aagtgaaacc ggaaaagctc cccatcatcc acatcacaat cttgtccatt
     6901 gaagcccgat tcaaaaccat aatcaccgac accatgctcg aggcaaccga cccgacagct
     6961 acaccggcaa ggaccagatc ctgcaccctc cgcaccaggc cgtttgttga gatggtgtag
     7021 acaagcaaag taacagccag agcccctaga aacgctgcaa acgagaaacc atagacgctt
     7081 gttatgggca aaagcgaagc tatggccgca cctaaactgg ctccagccga tacacccaat
     7141 atataaggat cggccaggga gttgcgcaaa actccctgga atgccgttcc cgaaagagct
     7201 aaaactgctc ccactaaaac tgctcctaca acccggggca gcctaatctg ccagaagatc
     7261 gtctcccagt aactagggcc ttccacttcc cggcccagga gcttaccaat aatccacttg
     7321 cccggaagct tcaccgctcc caaactagaa gcgacaataa ccgacacgat caaccctaca
     7381 aacagcagca caatcagctt attgaaggtt aaataacgct tcattcagtt cctcaaggcc
     7441 gtcccaaagc ctctttgttg ggaaggtgta aagagacggg ttgatctcta gcagtcggtc
     7501 tctaaaggat gcgtaaaccg gtctctgcat ggcttcctct aaattgaaat tggtaagaat
     7561 gatcaggtcg ggattctgcg cgattagatc ttcctcgttg aacaccggaa atgcacctag
     7621 tgcagaagct ccaacgttta tcccaccggc tttagtgatc acatcgtccc acagggttcc
     7681 cttcccagcg gtcattaaag gatcgttc
//
LOCUS       DAOSDA010000017         7432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_6416_length_7432_cov_3.135014, whole genome shotgun sequence.
ACCESSION   DAOSDA010000017 DAOSDA010000000
VERSION     DAOSDA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6416_length_7432_cov_3.135014"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLW82_00845"
     CDS             complement(<1..840)
                     /locus_tag="PLW82_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ45856.1"
                     /translation="MPNVAKQLQQQPGQGEPVIRVEDLHTWFEIRRMGFFKAGDVRAL
                     DGVSFELYQGETISIVGESGCGKSTLAKTILGIHHPTKGKIFFQGKDLSTLDKAGWKN
                     YRASVGYIQQDPYGALPPFMTVKRILEEPMKINGITDKKEQARRIRQVLEEVKLAPIE
                     DFLPKFPHMLSGGQQQRLVIARAMILGPKLLIADQPVSMLDASVRVEILQLLQGLQRR
                     HNLGIIYITHDLSTVRYFSERIFSMYGAKIIEKSSTQALLKEPLHPYTRALFEATSDP
                     DVEN"
     gene            complement(833..1816)
                     /locus_tag="PLW82_00850"
     CDS             complement(833..1816)
                     /locus_tag="PLW82_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ45857.1"
                     /translation="MSKVLLDVRNLKLYFRTRSGPVQAVDNVSFQLEEGKTLAVVGES
                     GCGKTSLTKAILRLLPRNVETYEGEIIFEGEDIMKYNEDKFRKEVRWSKIAMVPQASM
                     NSLNPVLRIEDQLMEPLLLHDRTMTKKKALDRVKEVFNIVGLPTDFLRRYPFELSGGM
                     RQRATIAMALAANPKLVVLDEPSSALDLLTQANLMNVLKRIKQEYKTTFILITHDIGT
                     ASELADEIAVMYAGEMVEYSSAEYFYTDAHHPYSRKLMASVPTLREDKELQFIPGQPP
                     SLINPPSGCRYAARCEKRFEKCSEHPCTVELEHGAKVQCWLYDETGEVKHA"
     gene            complement(1820..3082)
                     /locus_tag="PLW82_00855"
     CDS             complement(1820..3082)
                     /locus_tag="PLW82_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ45858.1"
                     /translation="MVCLAIYAVFAIPYEQAVKLWRGDDKVWADTPRNARPVWFNWFY
                     KEKQPVTINLNSAKDPSLKSVQDLGGGMSMVDVVFEFDYNYDGFPSEISLFFTSKFNS
                     ARPNVDITWITPDGREIPMSNMALSASNTYTISQDNALSRRLRGVAPEKGLFADPNDP
                     SKVVKGTYKMVIEGLVFEEGADYDAKLVVYGKLHGLAGTDHRRRDITIALLWGTPVAL
                     SFGLLAAVGSSLITMMLAAAAVWFGGWVDAVIRRINEIVMILPLLPILIMVGLFYSRS
                     IWAILGVVILLGIFGSSILSYRSMFLQVKQSGYIEAARAYGASSGRIIFRYMIPKVIP
                     VLIPGFVTQVPSYVFLEATLAVLNLGDPLLPTWGKLLNDAYSEGALFTGHFYWVLQPA
                     ILLVLTGLGFAMLGFALDRIFNPRLRGL"
     gene            complement(3160..4287)
                     /locus_tag="PLW82_00860"
     CDS             complement(3160..4287)
                     /locus_tag="PLW82_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ45859.1"
                     /translation="MANVETAEKQTVQKTQRTSGNLGRIAKYTLMKGITLLITVVIGV
                     YLAILIANGGGYVDKMREGQIREQVSLQLAGNPEMAYLSQAERQQIYEQTVQIRIRQV
                     GLDQPFMVRSFRYLWNGLTLNLGNAEYLLSDSGSKQVRNILGERLTPTLLLMASGQML
                     LFFTSVFFALFLSRRYGSVIDRIVVAMAPTSAAPAWFYGIFLILVFAALLGWLPFGGM
                     IDAPPPQNPWLRAVSVLKHMVLPVGAIVLSSIFLTSYYWRTFFLIYSSEDYVDMAKAK
                     GLSDRAIERRYVLRPTLPTIITSFALTLIGLWTGAIVLETVFGWPGIGRLTQSAINMF
                     DTPVIVGTVVIYAYLLALTVFLLDIIYALVDPRVKLGGGEK"
     gene            complement(4387..6846)
                     /locus_tag="PLW82_00865"
     CDS             complement(4387..6846)
                     /locus_tag="PLW82_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ45860.1"
                     /translation="MFKRRNIAILAVMLLVLAVSVFAEQRMGTWVDEVIIVEEPNVTT
                     AVSRLKAGDLQIWASGSSDINAFNAVKEDPNLDYLQVFGSYSEITFNPVGPKFNDGRL
                     NPFSSRKIREATNMMIDRDYIAQEIYNGLAVPKHNLLNTSFVDYSRVVETARSLEVKY
                     AFNLEKAKEIIHEEMVAMGATLVNGYWQYEGKDIELIVLARSEDERKELGEYFAEQLG
                     KVGFKTKVEYRTGAEASPIWLLGDPWQGQWHVYTGGWSAPVVYRDQGHIFNQMYTRRT
                     MSQPLFQALEPIPELDEISDKLYRKEFTTMEERNALYSRALELAYQDSPRIFVVDQTD
                     FIPKRKELAITADLAGGVSGTSLWPMTIRRGDQIGGTIRMGSNQVLVEPWNPVGGSNW
                     AQDQLAIRATFDRGFLVDPYTGNYHPLHIERVDVTVKEGLPVSKSPDSDWVNLEFAEE
                     IQVPSDAWAEWDPVNKRFITVAEKYPEGLTATRKSVVYYRPETFSEVKWHDGSPYTVG
                     DMLLAYVLDWDRAYEASEVFDESAVPGLQTALASFRGLKIVSLDPFIYEYYTESWSLD
                     AEQNISDLFAVYYNYGNAPWHTLALGMLAEINGELAFTKSKADQINAEWLGYNTGPSI
                     PILAKYLDQAIATNYLPYAEFLSEFIPEEEIALRYKNAKAWYEAKGHFWIGNGPFYLE
                     KAYPTERMVHLKRFEDYWLPAGYFDIFEEPRIAEVEVLGPRRVNAGTQASFQVDISFK
                     GEAYRLEDIQEVKYIVLDAAGNVAFSGYGTGYMDGYYEIVLSPEDTAKLPVGTNTLEV
                     IVLPTLVGGATFGSHTFVTLP"
BASE COUNT         1945 a         1795 c         1719 g         1973 t
ORIGIN      
        1 gttttccaca tcgggatcgg acgtggcttc aaaaagcgct ctagtgtaag gatgcagagg
       61 ttctttgagt aaagcctgtg tcgacgactt ctcgataatc tttgcaccgt acatggagaa
      121 aattctctcc gaaaagtatc ttactgtgga aagatcgtga gtgatataga taatcccgag
      181 gttgtgcctt ctctgaagac cctgcagaag ctgaaggatc tcaactctta ccgaagcgtc
      241 caacatggat acaggctggt cggcgataag cagctttggt ccgagaatca ttgccctagc
      301 aataaccaga cgctgttgct gacctccgct gagcatgtga ggaaactttg gaaggaagtc
      361 ttcaatagga gcaagcttaa cttcctcgag aacctgtctg attctccggg cttgctcttt
      421 cttgtctgta ataccgttaa ttttcatggg ttcttccaag atacgtttta cagtcatgaa
      481 cggaggaaga gctccataag gatcctgttg gatataacca acgcttgcac ggtagttttt
      541 ccaaccggct ttatctagag tactcaggtc cttaccctgg aagaagatct tgccctttgt
      601 agggtggtga atccccaaaa tggtcttggc taaagtactc ttaccacaac cgctttcacc
      661 aacgattgat attgtctcgc cttgatacag ttcgaaggaa acaccgtcca atgcgcggac
      721 atcgccggcc ttaaagaagc ccatgcggcg aatttcaaac caagtatgaa ggtcttccac
      781 gcgaataacc ggttcaccct gtcctggctg ttgttgtagt tgcttagcta cattaggcat
      841 gtttcacctc tcccgtctca tcatataacc agcattgcac ttttgctccg tgttctaact
      901 cgaccgtgca gggatgttca gaacacttct caaaacgttt ttcacaccga gctgcataac
      961 ggcaacccga aggtggatta atgagagatg gtggttgacc aggtatgaac tgtagttctt
     1021 tgtcttctct taacgtaggc acactggcca ttagcttgcg ggaatatggg tgatgagcat
     1081 ctgtgtagaa gtactcagca gagctgtact caaccatttc tccggcgtac ataacagcta
     1141 tttcatcagc tagttcacta gccgtaccta tatcgtgtgt gatcagaata aatgtggtct
     1201 tgtattcctg cttgatcctc tttagaacgt tcatgagatt ggcctgagtt aacaggtcaa
     1261 gagctgaaga tggttcatca agtacaacca gtttggggtt ggctgctagc gccatagcaa
     1321 tggttgccct ttgacgcata ccaccactta actcaaacgg atagcgacgt aagaagtctg
     1381 ttggcagacc aacgatgttg aaaacttctt ttactcggtc tagtgccttc ttcttcgtca
     1441 tggtgcggtc gtgcagaagc agaggctcca taagctgatc ttcgatgcgc aaaaccggat
     1501 taagcgagtt catcgaagct tgaggaacca tcgcaatctt gctccaccga acttctttgc
     1561 ggaacttgtc ttcgttgtac ttcataatgt cttcgccttc gaagatgatt tcaccctcat
     1621 aggtttcgac atttcgggga agcagacgaa gaatagcttt tgtcaaggaa gtcttaccgc
     1681 aacccgattc acctactacg gccagggtct taccttcctc gagctggaaa ctcacattgt
     1741 ctacagcctg aaccggtccg ctgcgagtac ggaaatatag tttaagattt cgaacatcaa
     1801 gtagtacttt gctcattcgt tataaccccc tcaatcgtgg gttgaagatg cggtcaagag
     1861 caaatcctag cattgcgaat cctaatccgg ttaacaccaa aagaatcgca ggctgcaata
     1921 cccaatagaa gtgaccagta aagagcgcac cttctgagta agcatcgttg aggagtttac
     1981 cccatgtagg aagcaacggg tcgcccaggt tgaggacagc cagtgtagct tccaagaaga
     2041 catacgaagg tacttgcgta acgaaccccg gaattagaac ggggataacc ttaggaatca
     2101 tgtagcggaa gataattcta ccgctgcttg ctccgtatgc ccttgcagct tcaatgtagc
     2161 cggattgctt tacctgcagg aacatcgaac ggtagctgag aatgctcgag ccgaagattc
     2221 caagcaggat aactacacct aaaatagccc atatacttcg agaatagaac aaccctacca
     2281 tgatcagaat cgggagtagc ggtagaatca taacgatctc gttgattctg cgaataacgg
     2341 cgtcaaccca tccgccaaac caaactgccg ctgctgccag catcatggta atcagcgaag
     2401 aaccaaccgc tgccaagaga ccgaatgaca gcgccacagg tgttccccat aaaagggcaa
     2461 tggtaatatc tctgcggcgg tggtcggtac ctgccagacc gtgaagtttg ccgtagacca
     2521 caagctttgc atcgtagtcg gcgccttctt cgaagacaag gccttctata accatcttat
     2581 aggttccttt gacgaccttg ctcgggtcat taggatcggc aaacaagcct ttttcaggcg
     2641 ccactccgcg aagcctacgc gaaagagcgt tatcttgaga tattgtgtaa gtgtttgacg
     2701 ccgaaagcgc catattgctc atggggattt ccctaccgtc aggggtgatc catgttatat
     2761 ccacgtttgg acgagcactg ttaaacttcg aagtgaaaaa cagtgagatt tccgatggga
     2821 agccatcgta gttgtagtcg aactcaaata ctacatcgac catggacatt ccgccgccca
     2881 gatcctggac actcttcaga ctagggtctt ttgctgagtt caggttaatc gttacaggtt
     2941 gtttttcctt atagaaccag ttgaaccaca cggggcgagc attgcgaggt gtgtctgccc
     3001 atactttgtc atcgcctctc caaagcttta cagcttgctc ataaggaatg gcgaacacag
     3061 catagatagc taggcagacc atcaagaaga tgatggcgag tccagcgata gctgatggat
     3121 agtcttttag ttgcttgaag aagttcgcta taaccttcac tatttttcac ctccaccaag
     3181 ttttactcgt gggtcaacta aagcgtagat gatatcgagt aaaaacacag tcaaagcaag
     3241 taaataagcg taaataacaa ccgtaccgac aataaccgga gtgtcaaaca tgttgatcgc
     3301 cgactgagtc agtcttccga ttcctggcca gccaaaaacg gtttccaaaa cgatagcacc
     3361 agtccaaagt ccaatcagtg tcagagcaaa actggtgata atcgtaggta gagtcggacg
     3421 caaaacgtac cttctttcga tagcacggtc tgagagacct ttggctttag ccatgtcaac
     3481 ataatcctca ctcgagtaaa tcaagaaaaa tgtgcgccag tagtacgatg tgaggaagat
     3541 cgagctcagt acaatagctc ctaccggcag aaccatatgt ttgagcacgc tcacagcacg
     3601 cagccatggg ttttgtggcg gtggcgcatc aatcatacca ccaaatggta gccaacctaa
     3661 aagagcagcg aaaaccaaaa taagaaatat gccataaaac catgccggtg ctgcactagt
     3721 tggggccatc gcaactacga ttctgtcaat aacacttcca tagcggcgtg aaaggaacaa
     3781 tgcaaaaaat acactggtaa agaacagcag catctgccca cttgccatca atagcaacgt
     3841 tggcgttagg cgttctccga gaatgttccg aacttgtttt gagccgctat cgctgagtag
     3901 gtattcagcg ttacccaggt taagagtcag accgttccac aggtagcgga aacttctcac
     3961 cataaatggt tggtccagtc caacctgcct aatgcggatt tgtaccgttt gttcgtagat
     4021 ctgctgtctt tccgcttgag ataagtaagc catttccgga ttaccagcaa gctgcaatga
     4081 aacctgctcc ctgatctggc cttcacgcat tttatcaacg taaccgccac cgttggcgat
     4141 caaaatagcc aaatatacac ctatcacgac agtaatcagc agagttattc ccttcattag
     4201 ggtatatttt gcgatgcgcc ccaggttacc gctagtacgt tgtgtttttt ggacagtttg
     4261 cttttcagct gtttctacat ttgccattta catacacctc ccggtaatta cctggaacaa
     4321 tacaaacagg agggcgtgga cgccacgccc tccgcggatt caccatattg cttttttagt
     4381 tagcgattat ggtaaggtta cgaatgtgtg tgaaccgaag gtcgcgccac caacgagtgt
     4441 tgggagaacg atgacttcga gggtgttggt acctacaggc aacttagctg tatcctctgg
     4501 gcttagaacg atctcgtagt aaccatccat gtagccagta ccgtagccgc tgaatgcaac
     4561 gttgccagct gcatcgagca cgatgtactt gacttcttgg atatcttcga gtctgtaagc
     4621 ttcgcccttg aaggagatgt caacctggaa ggaagcctgt gtaccggcgt tcacacgtct
     4681 tggtccgaga acttcgactt cagcaatgcg tggctcttcg aagatatcga agtaacctgc
     4741 tggcagccag taatcctcga aacgcttcag gtgaaccatt ctctcggttg gatatgcctt
     4801 ctcgagatag aatggaccgt tgccaatcca gaagtgacct tttgcttcgt accatgcctt
     4861 agcattcttg tagcgcaacg caatttcttc ctcaggaatg aactcggaga ggaactctgc
     4921 gtatggcagg tagttggttg cgatagcttg gtcgaggtac ttagcaagga taggaatgct
     4981 tggaccggtg ttgtagccca gccactcagc attgatctgg tcggccttgg acttggtgaa
     5041 ggccagctcg ccgttgattt cggccagcat acctagagcc aatgtgtgcc atggagcgtt
     5101 accatagttg tagtaaactg cgaacaagtc ggaaatgttc tgctctgcat cgagcgacca
     5161 agactcagta tagtactcgt agatgaatgg gtcaagggaa acgatcttga gaccacggaa
     5221 ggatgcgagt gctgtctgga gtccaggtac tgcagactca tcaaagactt cactagcttc
     5281 gtatgcacgg tcccaatcca atacgtaagc gagcagcatg tcgccaacgg tgtatgggct
     5341 accatcgtgc cattttactt cgctaaaggt ctctgggcgg tagtaaacta cgctcttgcg
     5401 tgttgcagtg agtccttcag gatacttctc tgcaacagtg ataaacctct tgtttacagg
     5461 atcccactct gcccaagcat cgcttggaac ttgaatttct tcagcgaact caagatttac
     5521 ccagtcggaa tctggagact tggacactgg caatccttct ttaacagtta cgtccactct
     5581 ctcaatgtgc agtgggtggt agttgcctgt gtatgggtct accaagaaac cacgatcgaa
     5641 tgttgctctg atggccaact ggtcttgtgc ccagttagaa ccgccaacag ggttccaagg
     5701 ctcaacgaga acctgattgg atcccattct gatagtgccg ccaatctggt cgccgcgtct
     5761 gatggtcatc ggccagagcg atgtaccgga gacgccgcct gcaaggtcag cagtgatggc
     5821 gagctctttt ctcttcggaa tgaagtcagt ttggtcaaca acgaagattc ttggagaatc
     5881 ttgatatgcc agttcgagtg cacgggagta gagagcgttt ctctcttcca tagttgtgaa
     5941 ctctttgcgg tagagcttgt cggaaatctc gtcaagctca ggaattggct cgagtgcctg
     6001 gaagagaggc tggctcatgg ttctgcgtgt gtacatctgg ttgaagatgt ggccttgatc
     6061 acggtaaact actggagcag accagccacc agtgtatacg tgccattgac cttgccatgg
     6121 atcaccgagg agccagattg gtgatgcttc tgcgccggtg cggtactcga ctttggtctt
     6181 gaaaccaact ttaccgagtt gctcggcgaa gtactcacca agttctttgc gctcgtcttc
     6241 gctacgtgcg agaacgataa gttctatgtc tttgccttca tactgccagt aaccattcac
     6301 cagggtggcg cccatggcta ccatttcttc gtgaatgatc tctttggctt tttcaagatt
     6361 gaaagcatac ttgacttcga gagaacgtgc tgtttcaact acacgtgaat agtctacgaa
     6421 agaggtgttc aagaggttgt gctttggaac tgccaagccg ttataaatct cttgagcaat
     6481 gtagtcacgg tcgatcatca tgttagtagc ttcgcgaatc ttgcgtgagc taaacggatt
     6541 gagacgacca tcattgaact taggtccaac tgggttgaac gtaatctcgg agtaggatcc
     6601 gaaaacctgt aggtaatcga gattcggatc ttccttgact gcgttaaacg cgttgatatc
     6661 agatgaacca cttgcccaaa tctgcaggtc tccagctttc aatctggata cagcggtggt
     6721 tacgtttggt tcctcgacga tgatgacttc gtctacccaa gtacccatgc gctgctctgc
     6781 aaagacacta acagccaaaa ccaacaacat aactgctaga atagcaatgt ttctgcgttt
     6841 aaacaaacct agtcaccttc cttataagag aactgtattg acacaaaact aaatcataac
     6901 tctccgaccc caaacctcag agcgtcaccc ccttgaattt taggtcgtga tttacggttt
     6961 ttgcgtttca agtcaagtaa tggtcgttat aacactattc tatccaactc gcggatttcc
     7021 tgcttgtctt cgcgaattag acagccacca ctcaaaacgt aaaatccgct tacccttaat
     7081 cacaaaccca ctgccaaatc gaaaaagcta agttgtaatc gcccaatact acaccacatc
     7141 aatataccct aggttcaaca gatctcggag ctctcatact tacccaccct aggatataaa
     7201 gtaatattat ctttgttttg ggcaatttcc ttcttaacta attcgattct tgttcaaaaa
     7261 atccttgtaa tctactaact tcgtattttg catcggtctc aagagcccca aaacctgttt
     7321 ctaccttttt attattatgc aatataatgc tagccttggc aaatacaaat tttagttttt
     7381 ttgtggttat cttgtgatag tgtcagggct gagatgtttt gggaatatgt ca
//
LOCUS       DAOSDA010000018         7312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_6535_length_7312_cov_3.110514, whole genome shotgun sequence.
ACCESSION   DAOSDA010000018 DAOSDA010000000
VERSION     DAOSDA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6535_length_7312_cov_3.110514"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLW82_00870"
     CDS             <1..345
                     /locus_tag="PLW82_00870"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOJ45861.1"
                     /translation="NSPWLSSFAIGDIFTVPFSHSEGKLVLSKDQAEVLFEKGQVAFQ
                     YADFEGNPPMTSPMNPSGSEYAIEGIVSSDGLVLGKMCYSERLDDNLYKNIPGTKHQD
                     IFANAVRYFQGK"
     gene            368..1075
                     /locus_tag="PLW82_00875"
     CDS             368..1075
                     /locus_tag="PLW82_00875"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008425032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOJ45862.1"
                     /translation="MEKRKLLYEGSAKEVYLTEDPDLVILHFKDDATAFSGIKKAEIA
                     GKGIYSNKISGIIFERLEKKGIPTHFVRLLSDREQVCRKVSIIPLEVIVRNIAAGSMA
                     KRLGIPEGTRIENTIYELCYKSDELGHPLINDHHAVFLGAASYQELELIYELTTRINR
                     ELTELFAAVGVDLVDLKLEFGRAADGSILLADEISPDTCRFWDAKTGAKLDKDRFRRD
                     LGRVKEAYEEVLNRLTK"
     gene            1091..2521
                     /gene="purF"
                     /locus_tag="PLW82_00880"
     CDS             1091..2521
                     /gene="purF"
                     /locus_tag="PLW82_00880"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOJ45863.1"
                     /translation="MYRLFAGNLNEECGVLGVYGVENAKDLMYYGLHTIQHRGQEGAG
                     IVTVDKNGVMHRVKGMGLVTEVFNAENLRSLSGDMAIGHVHYTVGNKTGIENIQPFLF
                     HHYTGDFAICHNGSVVNSNELRRFLEDQGSIFQSTSDSEILAHLIKREQPTAITVNVI
                     LEALNKLEGAFAYLIMTRDKLYCCRDKHGLRPLSIGTLGSGYVVSSETCVFEVTGAKY
                     LRDVEPGEVVVLDREGLHSYNYSMYKRHYMCAMEFVYFSRPDSDIEGLNVHTFRKETG
                     KLIYRDLPVDDADIVIGVPDSSLSAAIGYAEASGVPYEMGLIKSKYIGRTFIQPSQEL
                     REKGVRMKLSAVPSIVKDKKVVLIDDSIVRGTTSKRIVRLLREAGAKEVHVRIASPQL
                     VNPCFYGVDTTSHDDLICRHHTDDEVCKMIGADSLAFLSVDALYEAGRRSELCTACFD
                     GNYPTQLYSSIEIESKKESEVVKGGK"
     gene            2511..3542
                     /gene="purM"
                     /locus_tag="PLW82_00885"
     CDS             2511..3542
                     /gene="purM"
                     /locus_tag="PLW82_00885"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOJ45864.1"
                     /translation="MANSYEKAGVNLEAGYEVVRRIKKHVASTSRLGVTSDIGSFGGL
                     FDLAALNVEDPILVAGTDGVGTKLKIAFAMDRHDTIGIDAVAMCVNDVLAQGAEPLFF
                     LDYIAIGKNDPAKVEAIVSGIAAGCRQANCALIGGETAEMPGMYHEGEYDIAGFATGV
                     VSRSKLIDGSKVAPGDVLLGLESSGVHSNGFSLVRKVVADRGLDLNQRYEDLDPEKTL
                     GEVLLTPTNIYVREVLAVLKEVEVHGIAHITGGGFDENLPRSLQPGQGIFVREKSFET
                     LPIFRFLEEHGQIGHREMFNIFNMGIGMVLILQEKDAPKAQEILKSFGRKSWVIGEVT
                     DKEGVIIEP"
     gene            3539..4126
                     /gene="purN"
                     /locus_tag="PLW82_00890"
     CDS             3539..4126
                     /gene="purN"
                     /locus_tag="PLW82_00890"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOJ45865.1"
                     /translation="MKRIAVFASGNGSNYEAIEKAFENHRYCRVELLVCDNPKAYVLE
                     RAKKYGRQVFVFNPKDYESKEHYEREILQQLQLHQIDLVCLAGYMRIVGSTLLDAYAK
                     RIINIHPSLLPAFKGKNGIKDAFEYGVKVFGVTVHYVSPEVDGGKIIAQRAIEYHGES
                     LEELEGLIHQIEHQLYPETIEKLVKEWENEGLNQC"
     gene            4104..5669
                     /gene="purH"
                     /locus_tag="PLW82_00895"
     CDS             4104..5669
                     /gene="purH"
                     /locus_tag="PLW82_00895"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOJ45866.1"
                     /translation="MKALISVSDKTGVIEFARELVGLGFEIISTSGTMKALKDAGIPV
                     TNITDVTGFPEILGGRVKTLHPKVHGGLLAKREDQEHLAELKENHIEFIDLVCVNLYP
                     FVKTIAKPDVTMDEAIENIDIGGPSMLRSAAKNYKYVTVVCDPTDYKLVLAEIKEYGN
                     TTAETRLRLSAKAFTHTADYDAAISTYMREKAGLPEVLRLRFEEKQALRYGENPHQKA
                     KFYREADDIGYSLATAVQIQGKELSYNNIQDANAALSIVREFGRDVPFVVGMKHMNPC
                     GAAIGTDLKDAWTKAYKADPVSIFGGIVATNQEIGREEAELMMYTIPEEGQEKTSLFL
                     EVILAPKFTDEAKTAFAQRKNLRLLEFRMEDVKEGKQYVSVNGGLLVQDQDLEIKELS
                     AEMAVTKVKPTEEQLADLFFAWKIVKHVKSNAIVVAKDGQTVGVGAGQMNRVGSAKIA
                     IEQAHAAGHTQGLVLASDGFFPFGDTALYAAENGVVAIVQPGGSIRDEESIKVCDEKG
                     VSMLFTGMRHFKH"
     gene            5669..6919
                     /gene="purD"
                     /locus_tag="PLW82_00900"
     CDS             5669..6919
                     /gene="purD"
                     /locus_tag="PLW82_00900"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOJ45867.1"
                     /translation="MKVLVVGSGGRCHAIIDALKRSTESVEVFAAPGNDGMRPMAELV
                     PIPVTEVEQLRDFAKENRIDLTIVGPEAALEKGIVNVFQEAGLKIFGPTKEAARIETS
                     KSFAKELMQKYGIPTAAFKTFTDYQDAAYYVESLGRLPVVIKYDGLAAGKGVVIAETL
                     EQAKKTLYDMLVKDVFGKASVVIEEYLEGVEFSFMCFVDRENVYPMVLAQDHKRAFDG
                     DEGPNTGGMGAYSGVPVISQEDVDEALERIMKPVAKALVEEGVPFQGVLYGGLIKTAD
                     GVKVIEFNARFGDPEAEVVIPRLKTDLLEVILNVVEGRKVETQWSDAYTVGIVLASKG
                     YPGRYETGHPIRGLDRVEGLVYHMGTKFDGKEYLTNGGRVLIVVGRGKTLREAQKKAL
                     ADCEKIQCDNLYYRRDIGFRSLSD"
     gene            7241..7312
                     /locus_tag="PLW82_00905"
     tRNA            7241..7312
                     /locus_tag="PLW82_00905"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7274..7276,aa:Val,seq:cac)
BASE COUNT         1904 a         1759 c         2072 g         1577 t
ORIGIN      
        1 aactcaccgt ggctgagctc ctttgcgatt ggcgatatct tcaccgtgcc cttttcccac
       61 agcgaaggga agttggttct ttccaaagac caggcagagg tgctgtttga aaagggacag
      121 gtggcgttcc agtacgcaga cttcgaaggc aatcctccaa tgacatcgcc catgaatccc
      181 tctggctcgg agtatgccat tgaaggtatc gtctcgtcgg atggtttggt actcggcaag
      241 atgtgctact ctgagcgcct agatgacaac ctgtacaaga acattccggg aacgaaacac
      301 caggatatct tcgcaaacgc tgtcaggtac tttcagggca aatagcagtt cgaccggagg
      361 cagactaatg gaaaaacgca agctactcta cgagggaagt gcgaaagaag tataccttac
      421 cgaagatccc gatcttgtga ttttgcactt caaggacgat gctacagctt tcagcggcat
      481 caagaaggcg gagattgccg gtaagggaat ctacagcaat aaaatctctg ggatcatctt
      541 tgaaaggttg gaaaaaaagg gcatacccac tcattttgtc aggctcctta gcgacagaga
      601 gcaggtgtgc aggaaggtca gcatcattcc tctcgaggtg atcgtaagga acattgctgc
      661 cggatcaatg gccaaacggc tgggcatccc tgaaggcacc agaatcgaaa acaccatcta
      721 cgagctgtgc tacaagagcg atgagttggg gcatcccctg attaacgacc accacgccgt
      781 gtttctcgga gccgcttcct atcaggagct cgagctcatc tacgaactta caactcgcat
      841 caatagggag ctcactgagc tttttgctgc agtgggagtg gaccttgtcg atctcaagct
      901 ggagttcggc agggcagctg acggaagcat tctccttgcc gatgagatct ctcctgacac
      961 ctgccggttt tgggacgcta aaaccggagc taaactggac aaggaccgct tccgccggga
     1021 tctcggcagg gtcaaagaag cgtatgagga agttttgaac cgtctaacta agtgacagaa
     1081 ggagagcgct atgtatcgct tatttgctgg aaacctaaat gaagagtgcg gagtcctcgg
     1141 cgtctacggc gtagaaaacg ccaaggacct gatgtactac ggtttgcata ccattcagca
     1201 ccggggtcag gaaggtgcag gtatcgtcac tgtggacaaa aacggcgtca tgcaccgggt
     1261 taaggggatg ggccttgtca ccgaggtttt caacgctgaa aacctcaggt ccctttctgg
     1321 ggacatggcc atcgggcacg tccactatac ggtggggaac aaaacgggga tcgaaaacat
     1381 ccagcccttt ttgttccacc actacaccgg agactttgcc atctgccata acggcagcgt
     1441 ggtgaactcc aacgagctca ggaggtttct ggaggatcag ggaagtatct tccagtccac
     1501 ctcagacagt gagattctcg cccacttgat caagcgggag cagccaacag ctatcactgt
     1561 gaacgtgatt ttggaggctt taaacaagct agaaggggct tttgcctatt tgataatgac
     1621 cagagacaaa ctctactgct gcagggacaa gcacggtctg cgtcctcttt ccatcggaac
     1681 tttgggcagc ggttacgtcg tctccagcga aacctgcgtt tttgaagtta caggtgcaaa
     1741 atacctgaga gacgtagagc caggcgaggt ggttgtccta gaccgggagg gactccacag
     1801 ctacaactac tccatgtaca agcgccatta catgtgcgcg atggagtttg tctacttctc
     1861 ccgtccggac agcgacattg aaggactcaa cgtccacacc ttccgcaagg agacagggaa
     1921 actgatctac agagatctgc cggtggacga cgccgatatt gtcatcggcg ttcccgactc
     1981 cagccttagt gccgccatcg gctacgccga agcatcaggg gtgccctacg agatggggct
     2041 catcaaaagc aagtacatcg gcagaacgtt catccagcct tctcaggagc tgagggaaaa
     2101 aggggtgcgg atgaagcttt ctgcagtccc cagcatcgtg aaggataaga aggtagtcct
     2161 catcgacgac tcgatcgtca ggggcaccac gtcaaaaagg atcgtcaggc tactcagaga
     2221 agccggggct aaagaggttc acgtgcggat tgcctcgccc cagctggtca atccctgctt
     2281 ctacggcgtg gacaccactt cacacgacga tctgatctgc cgccaccaca cagacgacga
     2341 agtctgcaag atgatcggcg cagatagctt ggcgtttctt tcagttgatg ctttgtacga
     2401 agcgggcaga agaagcgagc tttgtaccgc ctgctttgac ggcaactatc caacccagct
     2461 gtacagttcc attgagatcg agagcaaaaa ggagagcgag gtggtaaaag gtggcaaata
     2521 gctacgaaaa agccggcgtc aaccttgagg ctggatatga agttgtcaga aggatcaaaa
     2581 agcacgtagc gtcaaccagc cgccttgggg tcacaagcga tatcggcagc ttcggaggtc
     2641 tctttgacct tgcagctttg aacgtggagg acccgattct tgttgcaggg actgacggtg
     2701 taggcaccaa gttgaagatc gcctttgcca tggatcgcca cgacaccatt ggcattgatg
     2761 cggtagctat gtgcgtcaac gacgtgctag cgcagggtgc agagcccctc ttctttttgg
     2821 attacatcgc cattggtaaa aacgatcctg caaaggtgga agctatcgtg tccggtattg
     2881 cagcaggctg ccgtcaggcc aactgcgcgc tgatcggggg agaaactgcc gagatgccgg
     2941 ggatgtacca tgagggagag tacgatatcg ctgggtttgc caccggtgtc gtctcgcgaa
     3001 gtaagctgat cgacggctct aaagtcgctc caggagacgt gctcctcggg ctcgagtcgt
     3061 ctggggtcca ctctaacggc ttcagtttgg tgaggaaggt cgtcgccgat cggggccttg
     3121 acctgaacca gcgctacgag gatctggacc cggaaaagac cctaggagag gtactgctga
     3181 cacccacgaa catctacgtg cgggaagtgc tagctgtgct taaagaagta gaggtccacg
     3241 gtatcgccca tattaccgga ggcggttttg acgagaacct cccccgcagc ttgcagccag
     3301 gtcagggcat tttcgttagg gaaaagagct ttgagacttt gccgatcttc cggttcctgg
     3361 aagagcacgg ccagattggc catcgggaga tgtttaacat cttcaacatg ggcatcggta
     3421 tggtgcttat tctccaggag aaagatgcgc caaaggctca ggaaatcctt aaaagcttcg
     3481 gcaggaagag ctgggtgatt ggtgaggtca cagataaaga aggagttatc atcgaaccat
     3541 gaagcgcata gcggtgtttg catcgggcaa tggctcaaac tacgaggcca ttgaaaaagc
     3601 gtttgaaaac cacaggtact gccgggtaga gcttctcgtc tgcgacaacc cgaaagcgta
     3661 tgtcctagag cgcgcgaaaa agtatgggag acaggttttt gtcttcaacc caaaagacta
     3721 cgaaagcaaa gagcactatg agcgggagat tttgcagcag ctgcagcttc accagatcga
     3781 cctcgtctgc cttgcgggct acatgcggat cgtaggaagc actcttcttg atgcttatgc
     3841 taagaggatt atcaacatcc atccgtcgct gcttccagca ttcaaaggga aaaacggcat
     3901 caaagatgct tttgagtatg gggtcaaggt cttcggcgtc accgttcact acgtgtcgcc
     3961 ggaggtagat ggaggaaaga tcatcgctca gagagccatc gaataccatg gtgaaagtct
     4021 tgaggaactg gagggactga ttcaccagat cgaacatcag ctgtatccag agacaatcga
     4081 gaaactggta aaggagtggg aaaatgaagg ccttaatcag tgttagcgat aagaccggcg
     4141 ttatagagtt tgccagagag cttgtggggc tgggttttga gatcatctcg actagcggca
     4201 cgatgaaagc cctgaaagac gcgggtattc ccgtaaccaa catcacagat gttacgggat
     4261 ttccggagat actaggcgga agggtaaaga ccctgcatcc caaagtgcac ggcggccttc
     4321 tggcaaagag agaggaccag gagcacctgg cggagttgaa ggagaaccac atagagttca
     4381 tcgatttggt ctgcgtcaac ctgtatcctt ttgtaaagac gatcgcaaaa ccagacgtaa
     4441 ctatggatga agccatcgaa aacatcgaca tcggcggacc ttccatgctc cgttcggcag
     4501 cgaaaaacta caagtacgtt actgtagttt gcgatcctac agattacaaa ttggtgttgg
     4561 cagagatcaa agagtacgga aacaccaccg cggagacgcg gctgcgctta agcgctaagg
     4621 cttttaccca tacagccgac tacgatgcgg ctatctccac ttacatgagg gagaaggcag
     4681 gtcttcccga ggttctgagg ttgcgctttg aagagaagca agcccttcgc tacggcgaaa
     4741 acccccatca gaaggctaag ttctacagag aagcagatga catcggctac agcttggcga
     4801 ctgccgttca gattcaggga aaagagctct cctacaacaa cattcaggat gccaacgctg
     4861 cactgagcat cgtacgcgag ttcggacggg atgtgccctt tgtggttggc atgaagcaca
     4921 tgaacccctg tggcgctgca attggcaccg atctgaaaga cgcttggacc aaggcgtaca
     4981 aagccgatcc tgtgtccatc ttcggcggca tcgttgcaac caaccaggag atcggcagag
     5041 aagaggccga gctgatgatg tacactatcc cagaagaggg acaggaaaag acgagtttgt
     5101 tcttggaggt tattttggca cccaagttta ctgatgaagc caagacagcg tttgcacagc
     5161 ggaaaaacct gcgccttctg gagtttagga tggaagacgt taaagaaggc aagcagtacg
     5221 tctcggtaaa tggaggtctt ctggttcagg accaggatct tgagatcaaa gagctatcag
     5281 cagagatggc cgtaaccaag gtaaaaccta cagaggagca gcttgccgat ctgttctttg
     5341 cctggaagat cgtaaagcac gttaagtcca acgccatcgt ggtggctaaa gacggtcaga
     5401 ctgtaggcgt cggtgcagga cagatgaacc gcgttggctc ggctaagatc gccatcgaac
     5461 aagcccacgc cgccggacat acccaaggcc tggttctggc atccgacggt ttcttcccct
     5521 ttggcgacac ggccctatat gcagcagaaa acggcgtcgt tgcgattgtg cagcccggtg
     5581 gatccattcg cgatgaagag tctatcaaag tctgcgacga gaaaggcgta agcatgctct
     5641 ttaccggcat gcggcacttc aaacactgat gaaggtctta gtggttggca gtggcggaag
     5701 gtgccacgcc ataattgatg ccctaaaaag atccaccgaa tctgttgaag ttttcgctgc
     5761 accgggaaac gacggtatgc ggccaatggc agagcttgtt cctatccccg taacagaggt
     5821 agaacagctg agagactttg ccaaagaaaa ccgcatcgac ctgactatag ttggtcctga
     5881 agccgccctg gaaaagggca tcgtcaacgt gtttcaggaa gcaggattga agatattcgg
     5941 gccgacaaaa gaagctgcaa gaattgaaac tagtaagagc tttgctaaag agttaatgca
     6001 aaagtacggc attcctaccg cagcctttaa gacgttcacc gattaccagg atgccgccta
     6061 ctacgtcgag tctttaggac ggctgcctgt ggttatcaag tacgatggcc tagcagcagg
     6121 gaaaggcgtg gtcattgccg agaccttgga gcaggctaaa aagaccctct acgacatgct
     6181 ggtaaaagac gtcttcggca aggcgtctgt ggtcattgag gagtacctag agggagtaga
     6241 gttttccttt atgtgctttg tagatcggga aaacgtctat ccaatggttt tagcacagga
     6301 ccacaagcgg gccttcgatg gagacgaagg gccaaacacc ggcggcatgg gagcctacag
     6361 cggcgtgcct gttatcagcc aagaggatgt agatgaagcc ctagagcgga ttatgaagcc
     6421 agtggcaaaa gctcttgttg aagagggcgt gccgtttcaa ggcgtgctct acggagggct
     6481 gatcaagacc gcagacggcg ttaaggtcat cgagttcaac gcccgcttcg gagatcccga
     6541 agcagaggtg gtcattccca gactcaaaac tgaccttttg gaagtgatct taaacgttgt
     6601 tgagggcaga aaggtggaaa cccagtggag cgatgcctac actgtgggga tcgttctggc
     6661 cagcaagggc tacccaggtc ggtacgaaac aggtcacccc atcagagggc tagatagagt
     6721 tgaaggtctg gtctaccaca tggggaccaa gttcgacggc aaagagtacc tgacaaacgg
     6781 cggaagagtc ttgatcgtag tgggcagggg gaagactctt cgcgaagccc aaaagaaggc
     6841 tctggcggac tgcgagaaga tccagtgcga taacctctac taccgtaggg acatcggctt
     6901 ccggtccctt tcggactaag cggtacggct gcttcgaggg ccggatccgc tgcgcaaatg
     6961 cgattaacta tgtctaaaga agaagctgaa gacctggatt tgacaccggt cgttcagctt
     7021 tttttttgcc tcggtagagg agcgcgagaa aggcatgcac tgctgtactt gatggtcagg
     7081 ggcctgcgtt ttggaggggc tttctgtagc aagaaagtct ttaaatttcg ttaaattggt
     7141 agttatcatt ttgaaatgtt tgcggtataa ttacgtttag aaggagtgct taattaatgg
     7201 tcgcggcaag accgcaaagc agagcatcct ccagatttac gggcaattaa ctcagctggt
     7261 agagtgctac cgtcacatgg tagaagtcgt gggttcgagt ccctcattgc cc
//
LOCUS       DAOSDA010000019         7296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_6547_length_7296_cov_2.991161, whole genome shotgun sequence.
ACCESSION   DAOSDA010000019 DAOSDA010000000
VERSION     DAOSDA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6547_length_7296_cov_2.991161"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLW82_00910"
     CDS             complement(<1..198)
                     /locus_tag="PLW82_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45868.1"
                     /translation="MNMQALTEVSAIRKQTLRLKTVTPLLMHGDNNKQKAQTRGPSFK
                     GMLRYWWRAIQTESQGLLAAEI"
     assembly_gap    349..358
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<359..1475)
                     /gene="csx2"
                     /locus_tag="PLW82_00915"
     CDS             complement(<359..1475)
                     /gene="csx2"
                     /locus_tag="PLW82_00915"
                     /inference="COORDINATES: protein motif:HMM:NF021006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02221 family CRISPR-associated protein"
                     /protein_id="HOJ45869.1"
                     /translation="MGVHDYKPCYYKRGGYTTKKTCFAQVGLLEILTKEGHNIEEVVA
                     FATDEARAKNGDKLCAELRANGYENSCRFVKIVDKVTDDKIWDLFEAVYNEINPGDTI
                     FFDVTHGFRSFPITVLAVLYYSRVLDSIKIGGIYYGNFEAHEEIGEDMYAPLDELTDL
                     LAFLDWTYGIDTFLKTGSPRAIEPLVSTSESRREAATAEKSEESGSVLEALKGFTRSL
                     ETCKSISFQKHARNLKNTVRSSDLAGLPYCKPLEKLLDLVEKKMEPFTGDPIMDPYYA
                     VQWCIDHGLTQQAYTMLIEHVVTVLCLLTGENVSSTSKRLSVQRYLSEDELGRKLKEL
                     AEIRPRKRDAIMDLVEFFGRHSEIVESISNLKTGRNSL"
     gene            complement(2383..3636)
                     /gene="csx2"
                     /locus_tag="PLW82_00920"
     CDS             complement(2383..3636)
                     /gene="csx2"
                     /locus_tag="PLW82_00920"
                     /inference="COORDINATES: protein motif:HMM:NF021006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02221 family CRISPR-associated protein"
                     /protein_id="HOJ45870.1"
                     /translation="MLAINRLFSFIGTGRCLPCRYFKGEHVAQETPVVQLALLELLQK
                     EGHEIHEVVVFATDIAREKNGELLINEFQEKEVKTPYKLIPVVGSVTGDNIWDLFETV
                     YNEIQTGDTIYFDITHGFRSFPLAVLPMLHYSRELKKIDVGGIYYGNFEAGSTIEGVI
                     HAPLDELTDVLSFLEWTYGINTFLKTGSAEVISRLVNEEKKTNPLVSNCNTKVANSMQ
                     KFNRALETCRGISFQENAEELKAIVREAAQDTTGYHKLLRNLFNELDEKMAVFTSDKV
                     MDPFYAVKWCIDHGLTQQAYTLLNEHVITALCLLLNENCEDKNTRQIVSRAIHIISNK
                     DTRDSYEPSSEREGEIIKRTRALLGPHPEVISCLNTLKTYRNDLNHAEHVDSPAPSQR
                     FEIKIREIVVTLEPYFRYIQSTQKA"
     gene            4288..>7296
                     /locus_tag="PLW82_00925"
     CDS             4288..>7296
                     /locus_tag="PLW82_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOJ45871.1"
                     /translation="MTYAICLIFTDTAARRMLELKSRPQETYSTQQRDKAYEVLWEFV
                     EVGNPVQCIVQKARQDGHFEICIPSAKLVLRGAVEHDPKKFIIISVRSMDKRDAEAPW
                     NKTVTLQFYDERTIPSFVKGVDQSFWDEINALGSGKEFAARMTQQMEDWMHYLTILER
                     LAEQKQFSFQYDAFRSDLDWTEVEFKITKNESISGNQVKKLVNEKLHLEVSPNQPLPL
                     GTLIDYKNQTLYIELEDSVRRKILEGTFQIPESAFLVYKNRGTLTEIKRQRNAIKELI
                     TGRSRNKNLASLLFDLDEYFKDFISWQINTDDYDFINPNLDPYQKQAVVGALEAKDFY
                     LIKGPPGTGKTTVIAEICAQAARQGKKILVASQTNVAVDNVLEKFVGVPNIRAIRFKN
                     DQDYDEETDLFSPQNAVGQWLDRILAHSKKRWEEYESAPSLLKEIDHIFNTAKLYAAV
                     QERLSTFQTELSGVSQQLENNRLLIQENQGNLNYLNEMLRTADLLSTALSDRDLARFF
                     SLFRTWNDRELFQNRSATAFAEKMKLTITQMSKFFALGSHSLDSPYEVIQSALSSYLW
                     YKEQRQEVSFVISHLESAVQKLAFFPDVHKDYQVAQADLRERQNKYDRELAAYRANSE
                     KIEKTQSILLQLENLDTDPSHFQRWASWFAGSTNRLEDRAASQKLAIEKAPVLSNATG
                     EAKTRLADLTERRINNLSDSCLDYMEDRRNLQRALDEVRQIISSFEATDRELRAKVPN
                     PGFLSQTDSERLLRSKTKVNVINPSYPDARALANIKAELQQTIDGYLGKGLVGVASSV
                     WRTVSFGLSSLFDGQSMATKKEAFLKEGLMLCHGGKTFFSRLKAQWEEKERQSKENLD
                     RSTEQLASGSAVIVKETVERLIFEYREKISGFRVQQENQERVIQQLKDECLQLEQKSE
                     VILGTLDSIYAAGIAPLRAVGVWGTYPSLKKLVDALLQTSDYSQREKIYYELKQELQV
                     FLDLVRQLDSLDLSSELHPVIASAVREIESL"
BASE COUNT         1930 a         1699 c         1677 g         1980 t
ORIGIN      
        1 aatctctgca gcgagcaatc cctgggattc ggtttggatg gctctccacc aatagcgcag
       61 catgccttta aaagaaggtc ctctggtttg cgccttttgt ttgttgttat ccccatgcat
      121 gagtaaaggg gtaaccgttt tcaacctcaa ggtctgcttt ctaatagctg aaacctccgt
      181 taaagcttgc atgttcataa ggatcacctc tctttctttg catgtgtagt gttgcttcga
      241 aataaattcg ctttggcgta cctgaaaaac ggttccaaag ttttaaatat ctccgtgatt
      301 tccttatgga actcttcaga cgaaggctgc ttactggtgt attcagcgnn nnnnnnnntc
      361 aagctgttcc ggccggtctt caagttacta atactttcca ctatttccga atggcgtccg
      421 aaaaactcta ccaggtccat gattgcatca cgttttcgtg gacgtatttc agcaagttct
      481 ttgagctttc tccccaattc atcttcagag agatacctct gcacagacag ccgtttgctt
      541 gtactagaca cattttcgcc cgtcagcaaa cacaatacag ttacgacatg ctcaatcagc
      601 atagtgtagg cttgctgagt cagtccgtgg tctatgcacc actgcaccgc gtaatacggg
      661 tccataatcg ggtctccagt gaatggctcc atttttttct ctactaggtc cagcagcttc
      721 tctaaaggtt tgcaataggg aagacctgca aggtcggaag acctcacggt gttttttagg
      781 tttcttgcat gtttttgaaa gctgatgctt ttgcatgtct ctagagaacg ggtaaacccc
      841 ttaagcgctt ctaaaacgct gccgctttct tcgctttttt ctgcagttgc tgcctcgcgt
      901 cttgattcgc ttgtactgac caaaggctct atcgctctgg ggctcccagt cttgagaaac
      961 gtgtcgatac cataggtcca atccaaaaac gcaaggagat cggtgagctc atccagagga
     1021 gcatacatgt cttctcctat ctcttcgtga gcctcaaagt tgccgtagta gatgccgccg
     1081 atttttatac tgtccagcac acggctgtag tagaggacag ccaaaacagt gatggggaag
     1141 gaccgaaatc catgagtgac gtcgaaaaag atcgtatctc cgggatttat ctcattataa
     1201 acagcctcaa aaagatccca aattttgtcg tcggtaacct tgtccacgat ctttacaaac
     1261 ctgcaggagt tttcgtatcc gtttgctcgc agctcagcgc acaacttgtc cccgtttttg
     1321 gcgcgggctt catctgtagc aaacgccaca acctcttcga tgttgtgacc ttcttttgtc
     1381 aagatctcta acaagcctac ctgtgcaaaa caggttttct ttgttgtgta cccacctcgt
     1441 ttatagtaac aaggtttgta atcatgtaca cctataaacg aaaagacccg tttcgtaatg
     1501 gttacctcct ctctttcttt tgtgtccgaa gcccaatcct acctacctgc tactactgta
     1561 aggactctgt ccccaattca gtttgatgag attgagaaac cagaacggac gtctaaatct
     1621 actaggagaa aatgcgaagc atgcactagt ctctcaatcc ttttactacc ttgtgagaat
     1681 gtctctgtct ctgccgattc cgctctacgt ccctgtacct gaatgaccgc tcattttcaa
     1741 aagaggcaga ctgtggactt tctcgtttgt gttgaggaag gactcaggcc tccctgcgta
     1801 aactaatccg ctcatgaact gtactttgct taactacata ggtatttgcc tgtgcttgtc
     1861 cgaagctccc gacaaaggca cgaccaacag agccctatgc agtttgttcc gttaaaacaa
     1921 tatgtagagt gcgaatgcaa gtttcgttgc ttcggcttaa cctagttggg ttattaaaag
     1981 gacacctttt tttggcaagc ctgactttat tcgagcaggc cacctagagg cagagtactg
     2041 caccgttttg acggaaagaa ccgtcaccct ggttttccgc gagttttatg tgtctaagtg
     2101 tcccttttag aagtacatag catcaacgat gcttcgtgct ggttgcaagt tttcgctttg
     2161 ggttccgcac ttagaaaacc ctagccttca gtaccgttgg ttgcagaacc gagtcaagca
     2221 agcagcttga tcctgcgctg tatttaagca aaagggctac agccacacaa attgcggtgt
     2281 agcccttgtt cctgcttagt ccaacagcgt tctttttaaa ggcgtttgac taaccggatt
     2341 catgcggttt taatcgaaca acgttccgtg cgcgtttttg ctttaagctt tctgagtcga
     2401 ttgtatgtaa cgaaagtaag gttctaaggt aacaacgatc tctctaatct tgatctcaaa
     2461 tctctgagat ggtgcggggc tgtcaacgtg ttctgcgtgg tttaagtcgt tgcggtaggt
     2521 cttcagagtg tttaaacagg agataacctc tgggtgtgga ccgagcagag ctcgagtccg
     2581 tttgatgatc tccccttccc tctcagaaga gggttcgtag ctgtctctgg tatccttgtt
     2641 gctgattatg tggattgctc gactgacaat ctgccttgtg tttttgtcct cgcagttttc
     2701 gtttaaaagc aagcagagtg cggttattac gtgctcattg aggagcgtat aagcctgttg
     2761 ggtcaatccg tgatcaatgc accattttac tgcataaaac ggatccatta ccttgtcgct
     2821 ggtgaatacc gccatttttt cgtcgagttc attgaagagg tttctcagga gtttgtgata
     2881 gccagtggtg tcctgtgctg cttcccttac tatggctttt aactcctctg cattctcttg
     2941 gaaacttatg ccccggcatg tttccaaagc ccggttgaac ttctgcatgg agtttgcaac
     3001 tttcgtattg cagttgctta ccaatgggtt tgtcttcttt tcttcgttta ccagcctgct
     3061 gatgacctca gcactgcctg tcttaagaaa ggtgtttata ccataagtcc actccaagaa
     3121 agacaggaca tcggtcagtt catctagcgg agcatgaatc actccctcta tggtactgcc
     3181 ggcttcgaag ttcccgtagt agataccgcc tacatcgatc ttctttaact cgcggctgta
     3241 gtgcagcatg ggcaggactg caagagggaa agaccgaaag ccgtgggtga tgtcgaagta
     3301 gatggtgtct cccgtttgga tttcattgta cacagtctcg aagagatccc agatgttgtc
     3361 tcctgtcacg cttcctacaa cagggatcaa cttgtacggg gtttttactt ccttttcctg
     3421 aaactcgtta atcaacagct cgccgttttt ttctctggct atgtctgtag caaacaccac
     3481 aacttcgtgg atttcatggc cttccttttg cagtaactcc aaaagagcaa gctgaacaac
     3541 aggcgtctct tgtgcaacat gttcgccttt gaagtaccgg cagggtaagc atctacccgt
     3601 tccgataaac gagaacagtc tattgattgc taacaactcc ttccctgaca tttttgtgta
     3661 aaccttcagg aggtggcaga catcctcctg gctaagaacc caagttttgg gcttggttag
     3721 accctgtaaa cgcgaccttg ttctatgtac cagatttagg tgagtttgcc tgagtttctc
     3781 tgagccgaat gccccactca ccagactgct ttgctttttt attcaatatt cgcaggaaat
     3841 ctccttttct cattaaattt ttatattcat ctcactactg agaaaaaaac ccagaaagaa
     3901 cttgccgagt caatgtgggc aggtaagctc tgcagtttgc cgaaaactga tactagtagt
     3961 gtgtaagcat ctgttaaatt aggagtatac gagtacaaat cgacttacat aaatttccgt
     4021 aaagatccgt acagtttgag cgggcaatga gagaagtcgt ttactcttta ggatcttccg
     4081 agagtctata tccaaagatc cagtacctcc gtaaagagag acttggtaga gctcaaggta
     4141 ggagagtcgt gtatgcgaac gagtttaggt tctcgcaaaa attgcgttga ggaacaaccc
     4201 cgaaggtaca tttctaagtc cagctaggaa gctcaactcg tgtctcccgt ataaatgaaa
     4261 gattcaagaa aggaggcttg caacactttg acctatgcta tatgtttgat tttcactgac
     4321 actgcagcaa gaagaatgct agagctaaaa tcacggccac aagaaactta ttctacccag
     4381 cagagagata aagcctatga ggtcttgtgg gagttcgtag aagtgggcaa tccggtccaa
     4441 tgcatcgtcc agaaagcccg tcaagatgga cactttgaga tctgtattcc gtcggcaaaa
     4501 cttgtcctcc gaggagcggt ggaacacgat ccaaagaagt tcataatcat ttcagttcga
     4561 tcgatggaca aaagagacgc agaagctcct tggaacaaga ccgtgaccct ccagttttac
     4621 gacgagcgta cgattccttc ttttgttaag ggagtcgatc agagtttttg ggacgagatc
     4681 aatgctttag ggagcggaaa agagtttgca gccaggatga cccagcaaat ggaagattgg
     4741 atgcattacc tgacaatcct ggaaagactg gcagagcaaa agcagttttc gtttcagtat
     4801 gatgccttcc gctccgactt agattggacg gaagttgagt ttaagataac taaaaatgaa
     4861 tccatctcag ggaatcaagt aaaaaagctt gtaaacgaaa aattgcatct tgaggtaagt
     4921 ccaaatcaac cgctccctct tggaacgcta atcgattaca agaatcaaac tttgtacata
     4981 gaactagaag acagcgtccg ccgcaagatt ctcgaaggga ccttccagat cccggaatct
     5041 gcttttctgg tttacaagaa ccgaggaaca ctaacggaga ttaaacgcca gcggaatgct
     5101 ataaaagagc tgataacagg aagatctcgc aataaaaacc tggccagttt gctatttgac
     5161 ttagatgagt actttaagga tttcatctcg tggcagatca atacagatga ctacgacttc
     5221 atcaacccca acttagaccc atatcaaaag caagcggtgg ttggtgcttt agaagccaag
     5281 gacttctact tgattaaggg gcctccggga accggtaaaa ccacagttat cgccgagatc
     5341 tgcgcccagg cagctcggca gggcaaaaag atcttagtgg catcccaaac caatgttgct
     5401 gtagataatg tgctggaaaa atttgtaggt gtccccaata tcagggccat tcggtttaaa
     5461 aacgaccaag actacgacga agaaaccgat ctttttagcc ctcaaaacgc agttggacag
     5521 tggctggacc gcatcctcgc ccattctaag aagcgttggg aagagtacga aagtgcccct
     5581 agcttactta aggaaatcga ccatatcttt aatacagcca agctctacgc ggctgtccaa
     5641 gagaggttgt cgacgtttca aaccgagcta tctggggttt cccagcagct agaaaacaac
     5701 cgcctcctca tacaggaaaa ccaaggtaac ctcaactacc taaatgaaat gctgagaacc
     5761 gcagatcttc taagtactgc gttatccgac cgcgatctcg ccaggttttt ctccctgttt
     5821 agaacttgga acgataggga gcttttccaa aacaggtcag ctacggcctt tgcggagaag
     5881 atgaaactta cgatcaccca gatgagtaag ttttttgccc tggggagcca ttctttagac
     5941 agcccctatg aggttattca gtcggcttta agcagctacc tgtggtataa agagcagcgc
     6001 caagaagtga gctttgtgat ttcgcacttg gagagcgctg tgcaaaagct tgcgttcttc
     6061 ccagatgtcc ataaagacta tcaagtggcc caagcagact taagggagcg gcaaaacaaa
     6121 tacgatagag aactagccgc ttatagagca aactcagaga agatcgaaaa gactcagagt
     6181 atacttctcc agctagaaaa tcttgacaca gaccccagcc atttccaaag atgggcttcc
     6241 tggtttgctg gaagtaccaa ccggctcgag gatcgagcag catctcagaa gcttgccatt
     6301 gaaaaagccc ctgtgctgag caacgccact ggggaggcaa agaccaggct cgcagattta
     6361 actgaaagaa gaattaacaa cctaagtgat tcgtgcctag actacatgga agaccggaga
     6421 aatctgcaaa gagccttgga tgaggtaagg cagatcatca gcagctttga agctacggat
     6481 agagagttgc gtgccaaagt ccccaatcct ggtttccttt cccaaactga ctcggaaagg
     6541 ctcttaagga gcaagactaa agtaaatgtc attaatccgt cctatccaga tgccagggct
     6601 ctggccaaca taaaagccga gctgcagcag accatagatg gctatctagg caaaggtctt
     6661 gtaggtgtag caagcagtgt atggcgtacc gtctcgtttg gtctcagcag cttgttcgac
     6721 ggccaatcga tggcaacaaa aaaagaagcc tttctcaaag aaggcttaat gctctgccac
     6781 ggagggaaaa ccttcttctc gaggttaaaa gcccagtggg aagagaagga gaggcagtcc
     6841 aaggaaaacc tggatcggtc aacagaacag cttgcttcgg ggtctgctgt gatcgtgaaa
     6901 gaaaccgtag aacgtttgat ttttgagtac agagaaaaga tcagcggctt ccgtgtgcag
     6961 caggaaaacc aggaaagagt tatccagcag cttaaggacg agtgcttgca gctggaacaa
     7021 aagagtgagg tcattctggg tactcttgac tcgatctatg ccgctggcat tgctccccta
     7081 cgagcagttg gtgtttgggg cacatatccc agcttgaaga agcttgttga tgctctgctt
     7141 caaacttccg actactccca aagagaaaag atctactacg agttaaagca ggagctgcag
     7201 gtctttttag accttgtgcg gcaattagat agtttagact tgagcagtga gctgcatccc
     7261 gttatcgcca gtgctgttcg ggagatagaa tcactt
//
LOCUS       DAOSDA010000020         7256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_6593_length_7256_cov_3.393695, whole genome shotgun sequence.
ACCESSION   DAOSDA010000020 DAOSDA010000000
VERSION     DAOSDA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6593_length_7256_cov_3.393695"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..2099)
                     /locus_tag="PLW82_00930"
     CDS             complement(135..2099)
                     /locus_tag="PLW82_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOJ45872.1"
                     /translation="MLLLLMLAAVVYAADITPATRRLIPRPEKVENLDSFIDGRTVTW
                     YVGANTNVFPLAEYYSQFGGELVLADGKVPNAYFGTVEDLSTTPALASVLGEVPEDPE
                     GYYLEVTDERIVIIGRTDVGAFYGVQTLRQLTENNFLIVGAKIVDYPKIRYRAIMDDV
                     SRGPIPKLETMKQIVKTMSEFKLNMYSPYMEMQVLDGIQDESKTLTAEDYRELVEYAK
                     QYHVEVVPSLQTFGHCNGFLFLPEYAHLGNLPSGSSQLDPTNPEVYEFLEAQIAKWAE
                     LTDSQFINIGGDETWEITSGNSGMLVQQIGVEQVYLDHVLKVMEMVKNHGKTPLFWGD
                     MALAHPEIIENLPNDAIVLNWHYGGADSMPQRLEAFRSKGKTQWAAPGIDNWLRIFPF
                     YTSSNSNVMNFAKIGFEYGIEGLFTTMWDDDGDTLFGHNWYGLIFASEAAWKAGGLGT
                     SSINQRFDMAVFGEDLGIASIISALANTNYQTYYGYFNSSNLMFFKNPFADKEFYNLA
                     GSANQLLLVERRVLETLSNANPVRNGHILKTLGFAARRNGAFGRKILLAKEVIDTVTE
                     IEGTENTEVLRELAQKLFDFEIELRELYAEFEELYLEENKEYFLKELKQRYFTLRAYI
                     FTLASELENASYMRVVPSPEALGLPRATSR"
     gene            complement(2335..2425)
                     /locus_tag="PLW82_00935"
     tRNA            complement(2335..2425)
                     /locus_tag="PLW82_00935"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2389..2391),aa:Ser,seq:cga)
     gene            complement(2615..3073)
                     /gene="tadA"
                     /locus_tag="PLW82_00940"
     CDS             complement(2615..3073)
                     /gene="tadA"
                     /locus_tag="PLW82_00940"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003392733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOJ45873.1"
                     /translation="MNHEHYMDLARAGAKKAFEIGEVPIGAVVVRGDEVIALAHNHRE
                     TAKNALGHAELLAIDEACKKLGGWRLIGCTMYVTLEPCLMCAGAIINSRIERLVYATR
                     DPKAGAVDSLYDVLRDSRLNHQVEVIEGVLAEESSRLLKQFFQELRRAKG"
     gene            complement(3075..3896)
                     /locus_tag="PLW82_00945"
     CDS             complement(3075..3896)
                     /locus_tag="PLW82_00945"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOJ45874.1"
                     /translation="MKVLIAADMEGITGIADRSYVLYDGLNYELGRSLMESDVNAAIE
                     GAIAAGATEILVVDGHSGGKNIRITKLNPRAVLHSGSTRPFSMIAGYDKVDVAMFIGY
                     HAKLGTENAVLDHTYTSRLKLWINGIEAGESYMNALLCGNKNVPVALYSGDQALAEEV
                     RGFSPDSYQVVVKEAYGRSSAYSLHPEQSRILIRENAKKALEAWKAGAIKPVTTTEPV
                     EIKVQFIISEMAEQAAVLPGAERVDPLTVVSIQPTIELGIRAFVTMVALATAPVY"
     gene            4244..5539
                     /locus_tag="PLW82_00950"
     CDS             4244..5539
                     /locus_tag="PLW82_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45875.1"
                     /translation="MRWKIIDFATSALSDETERALEVYFDSQYKKPSDIPDEKMGPFL
                     DWYIHDYDIPDLGISVLEYFFENRAGLMDVERFLTSGWLASYNNAYQAIEVKPGYWIL
                     LQDIVTKQHYLVANPELSEMTPPWSIFVGRLVPLGEIFEFGFIHHTLPPTALSVLRDN
                     LISTYEYHTSGFSKRISYSVFAKELLSPNLPHFIPEIISIPDLNPKDALTDALLPYLE
                     LFNWICISFYVSIPDKKPESLNKLILSVREKNALNDLFDSWPNGLKASGKDLDTYLTW
                     LEFLDACLVIHYGKKPPYDFSSKTQLIAKRKATPAVFKPLSETRTKISQESEALEKIS
                     KILHEYMIGRYPAKQIQAAILLWQHYLGVASDIPEIRNPKTWAGAIEYCLSRLLAIPV
                     TQKSLAETYNVSAPSISRVYKIISKQIDLNSLDKNAFLS"
     gene            complement(5587..6279)
                     /locus_tag="PLW82_00955"
     CDS             complement(5587..6279)
                     /locus_tag="PLW82_00955"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007440376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HOJ45876.1"
                     /translation="MSVLDQLKGGLIVSCQAKEGSPFRMPVIMAAMAKAAEQSGAVGI
                     RANGYDDIKAIREAVKLPIIGILKKDYDGYDVYITPTFEDAKLVASAGADIIAIDATP
                     RNRPGGETLKDLIARIKAELNVLVMADCSTAEEGLEAAKLGADIVATTLSGYTEYSKH
                     TLDKGPDVEMLKVLVEKAGVPVIAEGRFNEPGDVENALRIGAHAVVVGTALTAPEWRM
                     KQFVEVTEPFRK"
     gene            complement(6307..>7256)
                     /locus_tag="PLW82_00960"
     CDS             complement(6307..>7256)
                     /locus_tag="PLW82_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOJ45877.1"
                     /translation="LSVIARLDGTILGAGLGGPANHLHEEGAKERCKKSIRDSLQGAM
                     KEAGLTEVAFEHVALGMTGGTPLMRSLVWEVLQDYVDEDHFQFEHDSVTAWAGGTGGK
                     PGVVVIGGTGSVALAVDSRGNRARIGGWGYIMGDEGSAYQIALMALSAATKGYEGRGP
                     ETILTKALLEYFNVDDLWDVHAKIYSGDMDRSDIARLSKLVGDGANKGDAVCLTILSH
                     AAHELAQLVIAAAKKVDFSDVSPVISTAGGVWKAGLPIWKPFELEVTAAIENAQVVEP
                     VYPPSIGALILALKGAGVEITTDVTDNIEKTRAIIERKK"
BASE COUNT         1803 a         1927 c         1557 g         1969 t
ORIGIN      
        1 tttgcgagcc aaaaacatta aacctaacaa aaaagggagc gttgctccct acttacttag
       61 tagttttgca acactccctc ttcatgtcaa tttttcttta atgtattact tggttttggc
      121 ctcagcattg ctttctacct gcttgttgct ctggggaggc ctagtgcttc cggggacgga
      181 acgacccgca tgtagcttgc gttttccaac tcggaagcca acgtgaaaat ataggcccgg
      241 agagtgaagt acctttgctt cagttctttc aaaaagtact ctttgttctc ttcaaggtac
      301 agttcctcaa actctgcata aagctctctc agttcgatct cgaagtcgaa gagcttttgc
      361 gcaagttcgc gcaggacttc cgtgttttcg gttccttcaa tctctgttac cgtgtctata
      421 acttctttag ccagcaggat cttgcggcca aacgcaccgt ttctcctagc tgcaaagcct
      481 aaagtcttta ggatatggcc attacgcacc gggttagcgt tggatagagt ttccaaaacc
      541 ctccgctcca cgagaaggag ctgatttgcg ctgcctgcta aattgtaaaa ctccttgtca
      601 gcaaaaggat tcttgaagaa catcaagttg ctgctattaa agtagccata ataggtctgg
      661 taattggtgt tagcaagggc agatataata cttgcgattc ccagatcttc gccaaagacc
      721 gccatatcaa aacgttgatt aatactgctc gttcccagcc caccagcttt ccaagccgct
      781 tctgaagcaa aaatcaaacc ataccagttg tgaccaaaca gcgtatcacc gtcgtcgtcc
      841 cacatggtag tgaagagtcc ctcaataccg tactcgaagc cgattttggc aaagttcata
      901 acgttgctgt tagaacttgt gtaaaacggg aatatgcgca accaattgtc gatgcccggt
      961 gctgcccact gggttttccc cttggaccgg aaggcctcaa gcctttgagg catactgtcg
     1021 gcaccaccgt agtgccagtt gagcacaata gcatcgttcg gtaaattctc gatgatttca
     1081 ggatgtgcaa gagccatgtc gccccagaaa aggggagtct ttccgtggtt tttaaccatt
     1141 tccatgactt tcagaacgtg gtcaaggtag acctgttcta caccaatctg ctgtaccaac
     1201 atgccgctgt ttccagaggt aatctcccag gtttcatctc cgccgatatt gatgaactgc
     1261 gagtcggtta gctctgccca tttagcaatc tgcgcttcta gaaactcgta gacctccgga
     1321 ttggtggggt caagctgcga gctgccggaa ggcaaattgc cgaggtgagc atactcaggc
     1381 aaaaacaaaa acccattgca atgaccaaaa gtctgaagcg aaggaacaac ctcaacatgg
     1441 tactgcttgg catactctac aagttctcgg taatcttcag cagtgagcgt cttactttcg
     1501 tcttgtatgc cgtcaagaac ctgcatctcc atgtaaggcg agtacatgtt cagtttgaac
     1561 tcgctcatgg tcttcacgat ctgcttcata gtctccagct tcggaatagg tcctcgggag
     1621 acatcgtcca tgattgctct gtagcgaatc ttcggataat cgacgatttt tgcccctact
     1681 atcaggaagt tgttttccgt aagctgacgc agagtctgta ctccatagaa tgcaccaaca
     1741 tcagtcctac ctatgatgac aatcctttcg tctgtcactt ccaagtagta tccctcggga
     1801 tcctcgggaa cttctccaag cacagaagct agagcgggag tcgtactcaa gtcctctact
     1861 gtgccgaagt aggcattggg aaccttcccg tctgccaaca caagctcccc gccaaactga
     1921 ctgtagtatt cagcaagcgg gaaaacattt gtgttagctc caacgtacca agtcacagtt
     1981 ctaccatcaa taaacgagtc cagattttca accttttctg gtcttggtat gagacgtctc
     2041 gttgccggag ttatgtctgc cgcatatacg acagctgcca acattaataa cagtagcaaa
     2101 gaaaactccc acctcttttt catttgtgac accatcctct ccctcggtct actattcacc
     2161 agctttcatt ctttccctct attgcgtgcc ctttgcagtg ctctctcaaa gaggccttac
     2221 agctatccat gcaacaacct cgagtatttg cagctgaaat ctgcgtttat ctcatattaa
     2281 caacaaaagc cagtctataa agactggctc ttttctatca atttggttta ataatggcgg
     2341 agaagggggg attcgaaccc ccgcgccggt tgcccgacct accgcatttc gagtgcggac
     2401 ccttcaacca cttgggtact tctccgtgca gattttgcga gtagggcacc cttgtttgcg
     2461 tctgtacccc tagattcaaa ttatactacg cctcagttgg tttttcaact agtaccaaga
     2521 catcaggtcc agtcctgtga gaaggacaca aacacccttt cagacgctgc caaaaacgga
     2581 tctgatattt gctgtgactc atacctgcac gcgattaacc cttcgctcta cgcagctctt
     2641 gaaagaactg cttgagcaaa cggctggact cttctgcaag cacgccttct atcacctcaa
     2701 cctgatggtt cagcctggag tctcgcaaga catcgtaaag cgagtcgaca gcgcctgctt
     2761 taggatctcg ggttgcataa accagacgct caatcctgct attgatgatc gctcctgcgc
     2821 acatgaggca tggttctaaa gtgacgtaca tggtgcagcc gataagcctc catccgccga
     2881 gttttttgca ggcttcatcg atggcaagta gctctgcatg accaagagca ttcttagcgg
     2941 tttctctatg gttatgtgca agagcaatga cctcatctcc tctaacaact acagctccga
     3001 tcggcacttc accgatctcg aaggcttttt tcgctccggc acgcgccagg tccatatagt
     3061 gttcgtgatt catactaata caccggagct gttgccagag caaccatggt aacaaaagcc
     3121 cttattccca actcaatggt gggttggatg gagacaacgg tcagtggatc gactctttct
     3181 gcccctggaa gaactgctgc ttgttcggcc atttcgctga tgataaactg cacctttatt
     3241 tccactggtt cagtagtggt tacgggtttg atagcgcccg ctttccacgc ctctagcgcc
     3301 tttttggcgt tttctcggat caaaatgcgg ctttgctctg gatggagcga gtacgccgac
     3361 gatctgccat aggcctcttt caccactacc tggtagctat cgggggaaaa ccctctgact
     3421 tcttccgcca aggcctgatc gccgctgtag agagcgaccg gcacattctt gtttccgcac
     3481 aagagtgcgt tcatatagct ttcccctgct tcgattccgt tgatccaaag cttcaacctg
     3541 gaagtgtaag tgtggtccaa aactgcattt tcagtgccta acttggcatg atagcctatg
     3601 aacatagcca catccacttt gtcataacct gcgatcatcg aaaacggcct ggtagaacct
     3661 gaatgcagaa ctgcccgcgg gttcagtttg gtgattctga tgttcttacc tccgctatga
     3721 ccgtctacca ccaggatctc ggttgctcct gcagctatcg caccctctat ggccgcattg
     3781 acgtcacttt ccataagaga tcgacctagt tcgtagttta agccatcgta caaaacgtaa
     3841 gacctatcgg cgatcccggt gatcccttcc atatccgcag caataagtac tttcatcctt
     3901 ttccctccaa aagcacagtg agtctttatt tgcaattctg ggaagactaa aaggtgtcgt
     3961 taacacctgc taaacaccgt taacctctta caggcgactt aagtcccgag aggtttcacc
     4021 tttaacttac atatttttct attagcagga ttgaacctct taaaactaga aattaactgt
     4081 gaatgatcat ccgagcgaga aaggtaccca aggtgggatc aagatgcaga attcttatca
     4141 atttaagtgc tcttgcggaa gcaacaaacg ctatatagat tgctgtctca ttaagcctga
     4201 ctcatgggac gaaatcctaa ataggcagaa gatagccaca tctttgaggt ggaaaattat
     4261 cgattttgcc acatcagctc tatcggatga gacagaaaga gcgttggaag tttatttcga
     4321 cagccaatat aagaaaccat cggatatccc cgacgaaaag atgggtcctt tcttagattg
     4381 gtatatccat gactatgata tcccagatct aggcatatcg gttcttgaat acttcttcga
     4441 aaaccgggca gggcttatgg atgtagagag gtttctcact tcaggatggc tggcaagcta
     4501 caacaatgct taccaggcca tcgaagttaa gcccggctat tggatactgc tccaggacat
     4561 tgtcacaaag cagcattact tggtggcaaa ccccgaactt tccgagatga ctcctccctg
     4621 gtccatattt gtagggagac tggttcccct cggggaaatc ttcgaattcg gattcatcca
     4681 ccatacacta cctccaaccg ctttgtcggt tctgcgagac aacctcattt ctacctatga
     4741 gtaccacacc agcggtttca gcaagcgaat cagttattcg gttttcgcaa aagagctcct
     4801 ttctcctaat ttgccgcatt ttataccgga gatcatctcc atccccgatc tcaatccgaa
     4861 agatgctctg acagatgcgc ttttgccgta tctcgagctc tttaactgga tctgcatatc
     4921 gttctatgta agcattcccg acaagaaacc cgagtctttg aataagctga tcttaagcgt
     4981 cagagagaaa aacgctctta acgatctttt cgacagttgg ccaaatggat tgaaagctag
     5041 cggcaaagat ctggatactt acctaacgtg gttggagttt ttagatgctt gccttgtgat
     5101 tcattacggc aaaaagcctc catatgactt cagcagcaag acgcagctca ttgccaaaag
     5161 aaaagcaact cctgctgtct tcaaacctct gagcgaaacc agaacgaaga tcagtcagga
     5221 gagcgaggct ctggagaaaa tctccaagat tctccacgag tacatgatcg gtcgctatcc
     5281 tgccaagcaa atccaggcag cgattctcct ctggcagcat tacttaggag tggcttccga
     5341 tatccctgaa ataaggaacc ctaagacctg ggctggagct atcgagtact gcctatccag
     5401 gctgttagcg attcccgtta ctcaaaaatc gttggcagaa acctataacg tatcggcacc
     5461 ttcgatatcc agagtctata aaatcatttc caaacagatc gacctaaact ctctcgataa
     5521 aaacgcgttc ttgagttaat cgaagtttcc gaaaaacaag aaaggcctgc ataagcaggc
     5581 ctttttctat ttcctgaacg gttcggtgac ttccacaaac tgcttcattc tccactctgg
     5641 agctgtgaga gcggtcccta cgactaccgc atgagcgccg attctcaagg cattttccac
     5701 atctccaggc tcgttgaagc ggccttcggc aataaccgga acaccggctt tttctacaag
     5761 cactttcagc atttccacat ctggaccctt atccaaagtg tgcttggagt actctgtata
     5821 accgctgagc gttgtagcaa cgatgtctgc accgagcttt gcagcctcaa ggccttcttc
     5881 tgcagttgag cagtcggcca taacaagtac gttaagttca gctttgattc tagcgatgag
     5941 gtctttcaga gtttcgccac ctggcctgtt tctgggcgtg gcatcgattg caatgatgtc
     6001 tgctccagct gaagctacaa gctttgcatc ctcaaacgta ggggtgatgt agacatcata
     6061 accatcgtag tctttcttca aaataccgat gataggcagc tttactgctt ctctaatcgc
     6121 ttttatatcg tcgtagccgt tagccctgat tcccactgca ccgctttgtt cggcagcttt
     6181 agccattgca gccatgatta ccggcatccg aaatggactg ccttcctttg cttggcagga
     6241 tacaatcaaa ccgcctttta gttgatccaa tacgctcact gaaaagactc ctctctgctt
     6301 aaaggtttac ttctttcttt ctataatagc acgagttttt tcgatgttgt ccgtaacatc
     6361 tgtggttatt tcaacacctg cacccttaag agccagaatc aaagctccga tagacggtgg
     6421 gtacactggt tcgaccactt gtgcattttc tatagctgct gttacttcta actcgaaagg
     6481 cttccagatc ggcagacctg ctttccaaac gccgccggca gttgatataa ccgggctaac
     6541 atcggaaaaa tccactttct tggcagcggc tattaccagt tgagccagtt cgtgagctgc
     6601 atgggacaga atggtaaggc atactgcatc acccttgttt gctccatcgc ccactaactt
     6661 ggacagtctg gcaatgtccg atctgtccat atcaccggaa taaatcttgg catgcacatc
     6721 ccagaggtcg tctacgttga aatactccag aagtgccttt gtgagaatcg tttccggtcc
     6781 tctgccttca taacctttgg tagcggcaga cagggccatc aaggcgatct ggtaggcgct
     6841 accttcgtct cccataatgt aaccccaacc gccaattcta gcgcggttgc ccctggaatc
     6901 tacagcaaga gccactgaac ctgtgccacc aataaccaca actccaggct ttcctccggt
     6961 acctccagcc caagctgtga ccgaatcgtg ttcaaactga aagtggtcct catccacgta
     7021 gtcttgcaaa acctcccaga ccaggctgcg cattaacggc gttccacctg tcatacccag
     7081 cgcaacatgc tcgaatgcaa cttctgtgag tcctgcttcc ttcattgctc cctgaagaga
     7141 gtccctgatg gattttttgc accgctcttt ggctccctct tcgtgaaggt ggtttgccgg
     7201 gcctccaagt cctgccccga gaattgtacc gtctagcctg gcaatgacgc tcagcg
//
LOCUS       DAOSDA010000021         7215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_6642_length_7215_cov_2.986173, whole genome shotgun sequence.
ACCESSION   DAOSDA010000021 DAOSDA010000000
VERSION     DAOSDA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6642_length_7215_cov_2.986173"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PLW82_00965"
     CDS             complement(<1..1073)
                     /locus_tag="PLW82_00965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ45878.1"
                     /translation="MEKEENLNVFASKHEPYWLSSIKREEHPGLSEDIDVDVAIVGGG
                     ITGITTGFLLKKAGLKVAVLEANRLFSGTTGHTTAKLTSQHGLIYDRLIATFGEERAK
                     QYAVANEEAIGFVEALVREKNIDCNFETLPAYIYTEDERYIERIKREVAAAETLGLSA
                     SFAEDLPLPFTVRGAVKFEGQAQFHPLKYLSALAREIPGDGSFIFENTLAVDVEDAKR
                     PVVVTKDGPKVTASFVVIASHFPFYDGLGLYFTKIYTDRSYVIAIEAEESLGEGVFLS
                     AETPVRSLRNIKDGRSELIIIGGENHKIGDQTGTNVHYRNLADFAKINFTVKKIHYRW
                     STQDCMTADGVPYIGFLTAKTPT"
     gene            1301..2359
                     /locus_tag="PLW82_00970"
     CDS             1301..2359
                     /locus_tag="PLW82_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ45879.1"
                     /translation="MSGKLKVGIIGVGGIAKGAHIPGYLNLSDKVEIYALCDIKPERA
                     EKRAKELGVEHVFTDFHDLVAMKEIDVVSVCTSNYAHAPATIAALEAGKHVICEKPMA
                     RNAQEAEEMVKAYKKSGKILTIGYQWRFDSDIQALKERIQYGEFGDIYFAKAIATRRR
                     GVPAHGDFLNLEKQGGGPLVDIGTHALDLTLYLMDNHDPEVVLGSSYNAIAKNGPLNN
                     WGNYTPDEYEVEDAAFGLVKMKDGATLSLETSWVMNITEDEIFGTEIYGSKAGAVFHY
                     GKPLTVVSEEYNRINVSQVVRRNLNISNYHAEIEHFIDCVINGKEPLVKPEEALAVMK
                     IQDAIYRSQETKEAVKIV"
     gene            2375..3463
                     /locus_tag="PLW82_00975"
     CDS             2375..3463
                     /locus_tag="PLW82_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ45880.1"
                     /translation="MQYRDFGNTGVKVSRLGFGGMRLPTENPDYAIELLREGFRLGIN
                     YVDTALYYLDGQSEVLIGKALKGFRDKVYVATKNPIEDASGANWRRRLETSLKKLDLD
                     YIDFYHMWAINWEKYTKLIDVKNGPMEEAFKAKEEGLIRHISFSFHDTPENLIKLIDT
                     GNFETMLVQYNLLDRSNEEGIAYAKSKGLGVAVMGPVGGGRLAAPSEEIKRLIPQGTK
                     SSAEMAFRFVFSNPNVDVALSGMSNLEMLRENVRIASMEEPLSQEEKDTLEEILREKQ
                     RLANLYCTGCNYCMPCPNGVDIPRNFTAMNYYRVYDLLDTAKDAYNKLKNEQKAAESC
                     IQCYQCEPKCPQKIEIVRQLEEVAKALA"
     gene            3623..4666
                     /locus_tag="PLW82_00980"
     CDS             3623..4666
                     /locus_tag="PLW82_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOJ45881.1"
                     /translation="MRKILVVAALLTVLVLGAQAEVLEMQKGWELVLENSATHRLAEI
                     DLEKAEIDHLRATAENLLDNLEANQKKADEAWKKANKTYRTAVQNLQVTYLENYVNMT
                     NLMNQLEIRKIRLKQAETAYENGLKKWEIKSISDSELRNLKLNLESAQSNLANTENQI
                     ELAKMDFAALVGSADWEFDFKLEFVPFAETLEDVLKDLPNLERVVQARDQLDTATANL
                     EEARTKGEAVLTIRERELEVEKAKINLKNTEEDQLKQLIRSYNTLKQSEKSYLNALDS
                     YEVEQKKYEVALKQHSAGVITDADLENSRIALMEQENSLRDSLLSYYKSYMSLQQLRE
                     RGEFSLEQFFADH"
     gene            4641..6002
                     /locus_tag="PLW82_00985"
     CDS             4641..6002
                     /locus_tag="PLW82_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOJ45882.1"
                     /translation="MSSSLQITKKLLCVLLVAFSLVALAVADEVKETISLQDVLEAKR
                     QSYQFAESTQAERSLEKTKASLYPNLSGSSRVTKAMGRDFQLSSSYTLSWNTPIGVNM
                     QLKFVPRPGAQDSAVSFSVSASVWPLMANSNGLVKLESGKKTLDRAERSDAQKLFNEE
                     LSLYQNYVKNQIQYEKLLMQEIDLKRRSDEYAAAEIEFQLGNITRSELEDKEIAYRSA
                     FYQYKSAQLNFERSWTEFCQSLGLSTDSVPERVVYDRIVYPDLVWDLERDIARAKEIS
                     LDVLKAKDALDEAKKAVEDSRYSFIDSSLGFSVNVKELGGNSTPSWSVNLSMGFNTND
                     IFFNFTYKENQERYQSAEENYLAALKQVETDITRMQEDWELMVYQTKTREMNFIKAKR
                     DLQAKKLQLEAGVIKESDYLDAERAAFERYLELLDQLVSLHVAANKAMFATGRTDRVV
                     IYQ"
     gene            5999..7099
                     /locus_tag="PLW82_00990"
     CDS             5999..7099
                     /locus_tag="PLW82_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ45883.1"
                     /translation="MKRKKLFILLVVLVVIAGGAGGVYYYLQQRKKPNQPFVDAFRMR
                     QNTVVATRGNIATYISSSGTLEPAANVTVAAETSGEIKELLVKKNDVVKEGDLLAVLE
                     NEQAELDYIIAKRDYEAALIDGDEITIRQKAIQLKNAELALQRTEIRAPISGTIVEVH
                     VDDKDRVASGTSIVQIVDMQNFKINLRISELEISDIKQDQNVIVTVSSLGNQTYPAKI
                     TNVGVLPVGQGDPVTYPVEVRLSASDDRLRPGMNCNIDIVTAQATDVVVVPVESVNRV
                     GGRSYVTVVDGDDTKVVEVQLGLSDGLNVAVLSGLEPYTTILRTNYSLYIDAAAELVG
                     ETQPQPGQSQNRGAPAVGVPQVQFRSITGGRF"
BASE COUNT         2076 a         1607 c         1905 g         1627 t
ORIGIN      
        1 atggtggggg tttttgctgt aagaaagccg atgtaaggaa cgccatctgc ggtcatgcag
       61 tcctgggtag accatctgta gtggatcttc tttactgtaa agttaatctt ggcaaagtcc
      121 gcgagattgc ggtaatggac attggttccg gtttggtcgc ctatcttgtg gttttcacct
      181 ccgatgatga tcagctcaga tctgccgtct ttgatgtttc ggagggaacg cactggggtt
      241 tcagcgctga ggaacacgcc ttccccaagg ctttcctcgg cttcgatggc aatgacgtag
      301 gatctatctg tgtagatttt ggtgaaatag aggccaagcc cgtcatagaa ggggaagtga
      361 gacgcgataa ccacaaagga tgcagtgact ttggggccgt cttttgtaac caccacaggg
      421 cgctttgcat cttcaacatc gacggcaagg gtgttttcaa aaataaacga gccatctccg
      481 gggatctccc gagccagagc ggacaggtac ttcaaagggt ggaattgggc ttggccttcg
      541 aacttcactg cgcctcggac tgtaaagggg agagggagat cctcagcaaa tgaagcgctc
      601 agcccgagag tctctgcagc tgccacctct cttttaattc tctcgatgta cctctcatct
      661 tcagtgtaga tgtaggccgg cagagtctcg aagttgcagt cgatgttttt ctccctaacc
      721 aaagcttcca caaaaccaat agcttcttca ttggctaccg catactgttt ggcccgctcc
      781 tcaccgaatg tggcaatgag tctgtcgtag atcaggccgt gttgtgaggt gagcttggct
      841 gtggtgtgtc cggttgtgcc gctgaaaagc ctgtttgctt ccaagaccgc aactttcaga
      901 ccggcttttt tcagtaaaaa gcccgttgtg attccagtga tgcctccgcc gacaatagct
      961 acatcaacat ctatgtcttc acttaaaccg ggatgctctt ccctcttgat ggaggagagc
     1021 cagtagggtt cgtgcttgct tgcaaagacg ttgagatttt cctccttctc cacgtttccg
     1081 cctcctttta cttctggagc catttaccaa tattcttagt tactttgctg tttttattct
     1141 gattagcgct tttaggagga ggtttgtcca aatggcagct ggcatgtttt cgccaaatgg
     1201 ggtacagtat taaggtggca aaagttcact ttccgagagg aaataggaaa ccttgtgagt
     1261 aaataatcag ttgaaaacgc actgaaagga gatcttgaaa ttgtcgggca aactaaaagt
     1321 aggaattatt ggagtaggcg gcattgctaa aggtgcccat attcccggat acttaaatct
     1381 cagcgataaa gtagaaatct atgccctctg cgatatcaaa ccggagagag cagaaaaaag
     1441 agcaaaggag ctaggcgtgg agcatgtctt caccgacttc cacgatctgg ttgccatgaa
     1501 ggaaattgac gtggtcagcg tttgcacttc caactacgct cacgctccgg caaccatcgc
     1561 cgctcttgaa gctggcaagc acgttatctg cgaaaagccc atggcaagaa atgctcagga
     1621 agcagaagaa atggtgaaag cttacaagaa gagcggtaag attctaacca tcggctacca
     1681 gtggcgcttc gattcggata ttcaagccct taaagaacgc attcagtatg gagagttcgg
     1741 agacatttac tttgctaaag ctatcgctac ccgcagaagg ggagttcctg ctcacggcga
     1801 cttcttgaac ctggaaaagc aaggcggagg tcctctggtc gatatcggca ctcacgcact
     1861 ggacttgact ctttatctta tggataacca cgatcctgaa gtggtcttag gttctagcta
     1921 caatgccatc gccaagaacg gtcctctcaa caactggggc aactacactc cagatgagta
     1981 cgaagttgaa gacgcggcct ttggtctcgt gaagatgaaa gacggtgcaa cgctgtctct
     2041 tgagaccagc tgggttatga acatcaccga agatgaaatc tttggcacag agatttacgg
     2101 ctcaaaagcc ggtgcagtct tccattacgg caaacctttg actgttgtaa gcgaagagta
     2161 caacagaatc aacgtcagcc aggtagtcag gagaaacctc aatatcagca actaccatgc
     2221 agagatcgaa cactttatcg actgcgtcat caatggcaag gagcctctgg tgaagcccga
     2281 agaggcattg gctgttatga agattcaaga tgctatctac aggtcccaag agaccaaaga
     2341 agcggtaaag attgtctaag taggaggatt ttgagtgcag tatagagact ttgggaatac
     2401 tggtgttaag gtgtccagac tcggatttgg cggcatgagg ctgcctactg agaatcccga
     2461 ctatgccatt gagctccttc gtgaagggtt taggctgggg atcaactacg tagacacagc
     2521 actgtactac ctcgatggac aaagcgaagt cttgataggc aaagccctca agggttttcg
     2581 ggacaaagtc tatgtggcta cgaaaaatcc tatagaagat gcaagcggtg ccaactggcg
     2641 gagacgcttg gagacttcgc tcaagaagct ggatttggac tacatcgact tctaccacat
     2701 gtgggctatc aactgggaga agtataccaa gcttatcgat gttaaaaacg ggccgatgga
     2761 agaggcgttt aaggctaagg aagagggctt gatccgccac atttcgttct cctttcacga
     2821 tacaccggaa aacctgatta aactcatcga tactggtaac ttcgaaacca tgctggttca
     2881 gtacaacctc ttggaccgaa gcaatgaaga aggcatagcc tatgctaaga gcaagggttt
     2941 gggcgttgct gtaatgggac ccgttggagg gggaaggctt gcagcaccat cagaagagat
     3001 aaagcggctc attccccaag gcacaaaaag cagcgccgaa atggctttta gatttgtctt
     3061 cagcaacccc aatgtggatg tggcactctc cggtatgagc aacttagaga tgctgcggga
     3121 aaacgttcgg attgcatcta tggaagagcc tttaagccag gaagaaaaag acacgttgga
     3181 agaaatactc agggagaagc agagactggc aaacctgtac tgcacaggct gcaactactg
     3241 catgccctgc cctaacggag tggatatacc gaggaacttc acggctatga actactacag
     3301 agtgtacgac cttctggaca cggctaaaga tgcctacaac aagctaaaaa acgaacagaa
     3361 agccgctgaa agttgcattc aatgctacca atgtgagcct aagtgcccgc agaaaattga
     3421 aatagtaagg cagttggagg aagtggcaaa agccttagcc taaactgcat agcaaaaaac
     3481 acccaagatc ttgggtgttt tttcttgaat ggagagctta aattgaacag atatcaagga
     3541 ttttgcaact aaaagggcaa gtttgtttat atgttgagaa agacggaggt gaattaggtg
     3601 tcagtttggt gacaccgatg ccatgagaaa aatccttgtt gttgcagcac ttcttacagt
     3661 acttgtgctc ggagcacagg cggaggtgct cgaaatgcaa aagggttggg aacttgtact
     3721 ggagaactcc gccacccaca gactagccga gatcgatctg gaaaaagccg aaatcgatca
     3781 tttgagagct acagctgaaa acctcctgga caatttggag gccaatcaga aaaaagcaga
     3841 tgaagcttgg aaaaaggcaa acaagaccta cagaacagct gtgcagaacc tgcaggttac
     3901 ctacttggaa aactacgtta acatgacaaa cctcatgaat cagttggaga tcagaaagat
     3961 ccgactgaag caggcagaaa ccgcttacga aaacgggttg aagaagtggg agatcaaaag
     4021 tatcagcgac tccgaactca gaaacctgaa gctgaacttg gagtcagcgc aaagcaattt
     4081 ggccaatacc gaaaaccaaa tagaactggc aaagatggat tttgccgcgc ttgtgggaag
     4141 tgcagattgg gagttcgact ttaaactgga gtttgtgccg tttgccgaga ctctagaaga
     4201 cgtcttaaaa gacctgccaa accttgagag agtggttcag gcaagagacc agctggacac
     4261 tgccactgca aacctggaag aggcaaggac taaaggcgaa gcggtactta ccattagaga
     4321 aagagaactg gaagtggaaa aagcaaagat caatctgaaa aacacagaag aggaccagct
     4381 caagcagttg atccgcagct acaacactct caaacagagc gaaaaaagct acctcaacgc
     4441 tttggactcc tatgaggtgg agcagaaaaa gtacgaggtg gctctcaagc agcacagcgc
     4501 cggcgtcata accgatgctg atctggaaaa cagcaggatc gccctgatgg agcaggaaaa
     4561 ctctctacgg gacagcctgc tcagctacta caaaagctac atgagcctgc agcagctaag
     4621 agagcggggg gagttcagtc ttgagcagtt ctttgcagat cactaagaag cttctctgcg
     4681 tgctgcttgt ggcgttttct ctagtggctt tggcagtagc agatgaagtt aaagagacta
     4741 ttagccttca agacgttctc gaagcaaaaa ggcagtcgta tcagtttgcc gagtcgactc
     4801 aagcagaaag aagcttggag aagaccaaag caagcctcta tcccaatctt tccggaagtt
     4861 ccagagttac gaaggccatg ggaagagact tccaactgag ctctagctac actttaagct
     4921 ggaacacacc tattggggtc aatatgcagc tgaagttcgt cccgagacct ggagcccaag
     4981 acagcgctgt gagcttcagc gtttccgcaa gcgtctggcc actcatggca aactcaaacg
     5041 gcctggtgaa gctggaatcc ggtaagaaga ctttagaccg agcagaaagg tcagatgccc
     5101 agaagctctt taacgaagaa ctttcactgt accagaacta cgtaaaaaat cagattcagt
     5161 acgaaaaact ccttatgcaa gagatagatt taaaacggcg gtctgacgag tatgccgctg
     5221 ccgagattga gtttcaacta ggaaacatca ccagaagcga gttggaagac aaggagatcg
     5281 cctaccgcag tgcgttctac cagtataagt cggcgcagct gaactttgag agaagctgga
     5341 ccgagttctg ccagtctctt ggtctcagca ccgactccgt tcccgagagg gtagtctacg
     5401 accggatagt gtatccggat ctagtctggg atctggaaag ggatattgcc agagccaagg
     5461 agatcagcct cgatgtcctc aaagccaaag acgccctgga cgaagcaaaa aaggccgtag
     5521 aggacagcag gtacagcttc atcgattctt ctttgggctt tagcgtcaac gtgaaagaac
     5581 ttgggggaaa ctccacacca tcctggtccg ttaacttgag catggggttc aacaccaatg
     5641 acatcttctt caacttcacc tacaaggaga accaagaaag ataccagagc gccgaggaaa
     5701 actacttggc tgctttgaaa caggtggaaa cggatattac ccgcatgcaa gaggattggg
     5761 agttgatggt ttaccagacg aaaacgagag aaatgaactt tatcaaagcc aaaagagacc
     5821 tacaggcaaa aaaactccag ctggaagcgg gagtaatcaa agaatcggac tatcttgatg
     5881 cagaaagagc ggcttttgaa aggtatctgg agcttctcga tcagctggtt agcttgcacg
     5941 tagctgcaaa caaggctatg tttgccacag gtcgcacaga tagggtggtg atctatcaat
     6001 gaagagaaag aagctcttca ttttgctggt ggttttggtg gtgattgctg gaggggcagg
     6061 tggagtttac tactacctac agcaacgtaa gaagccaaat cagccttttg tagatgcgtt
     6121 tagaatgagg cagaatacgg ttgtcgcaac gcgcggcaac attgccacgt atataagcag
     6181 ttccggaact ctcgagcctg cagccaatgt aactgtagct gcagagacca gtggggagat
     6241 caaagagctg ttggtgaaga agaacgatgt tgtaaaagaa ggcgaccttc tggcagtgct
     6301 ggaaaacgag caggcggagc tggactacat cattgctaaa agagattacg aagcagctct
     6361 catcgatgga gatgagatca ctataaggca gaaggctatc cagctgaaaa acgccgaact
     6421 ggcgctgcag agaacagaga tcagggcgcc gatctccggc accattgttg aggttcacgt
     6481 ggacgataag gaccgtgttg caagcggtac ctctatcgtg cagatagtgg atatgcaaaa
     6541 cttcaaaatc aacttaagga tcagcgagtt ggagatctca gatattaagc aggatcaaaa
     6601 cgtcattgta accgtttcgt ctttaggaaa ccagacttat cctgctaaga tcaccaatgt
     6661 aggcgtgctt cctgtaggac agggagaccc ggtgacctat cctgtggaag tacggctttc
     6721 cgcttcagat gaccgcctca gaccgggaat gaactgcaac atagacatcg tcactgccca
     6781 agcgacagat gtggttgtcg taccggtgga atcggtaaac agagtgggag gcagaagcta
     6841 tgtcacagtt gttgacggag acgacaccaa ggtggttgaa gtgcagctcg gcctttcaga
     6901 cggtttgaac gtcgcggttc tgtccggact tgagccttac accactattc tcagaaccaa
     6961 ttacagtctc tatattgacg cagcagcaga actggtaggt gaaactcaac cgcagcctgg
     7021 ccagtcccag aaccgaggag cccctgccgt aggcgtaccg caagttcagt tcaggtcgat
     7081 tacgggaggg aggttctagg ttgagcacgc agacagccat cgaagtacgg gacgttgtaa
     7141 gagtttacaa gctgggcagg gttgaagtgg ctgcccttaa gggcgtaagt ttcaaggtgc
     7201 ataaaggcga gatgc
//
LOCUS       DAOSDA010000022         7195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_6668_length_7195_cov_3.038095, whole genome shotgun sequence.
ACCESSION   DAOSDA010000022 DAOSDA010000000
VERSION     DAOSDA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6668_length_7195_cov_3.038095"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLW82_00995"
     CDS             <1..295
                     /locus_tag="PLW82_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HOJ45884.1"
                     /translation="RLRDYMFRTGELVRDVGTVLVGREAVEAGLVNAVGGLSDAIAKL
                     NELCGRKSAQQGFFADQFNYQPQRMRPPEYGPRPGVPQPVPPQFNPNPGGLYS"
     gene            295..486
                     /locus_tag="PLW82_01000"
     CDS             295..486
                     /locus_tag="PLW82_01000"
                     /inference="COORDINATES: protein motif:HMM:NF025401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlzJ-like family protein"
                     /protein_id="HOJ45885.1"
                     /translation="MFYTVLPLELIMEEEERTLEWKTVGTASLLVEPLGDGTYRLERI
                     EGAPLSWYRDYNPGDIIKL"
     gene            606..1439
                     /locus_tag="PLW82_01005"
     CDS             606..1439
                     /locus_tag="PLW82_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOJ45886.1"
                     /translation="MKKKTKGILFFVILAVIVVGAVIWLKWINRSRYIEAATDTLVRA
                     VEVDALCYRFEVPLYAPADGKIEQLVADNTRVPANTVVAMVGSYPVYAERAGTVSWRY
                     DGLEKLLIPGVIMVLKHDFLGDWDKPVKKVSSGDVVYKGDIVGKLVDNYSWYLIVYSN
                     VSVYRNEKVQVEIGGKIYEGVVQEVLSDNRFSVVINYYDSDVAGYRVLRKIKLYKEPL
                     RGVIIPQDLLIETDDGTYVKISYRNRERTQPVRVVGKWEDKVVVDGLPKGSLVVVSPA
                     R"
     gene            1451..2125
                     /locus_tag="PLW82_01010"
     CDS             1451..2125
                     /locus_tag="PLW82_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOJ45887.1"
                     /translation="MAIKENLLRIRENIEKTCLLCGRDPREVKLLAVSKTFPISSILE
                     AYEAGQRDFGESRVQEFVPKYQQLPDLSWHFIGHIQKNKLNKLLDTEVFIHSVDSLEL
                     AEKISEKALERQKVVKVLLQVNIDDDPSKFGFTSEGLLQNIEKILQLKGIAPTGLMTI
                     GALHKDPKETRASFAKLRGLRDALSEVTKHEFPELSMGMSGDYHEAILEGATIVRVGT
                     GVFGQR"
     gene            2147..2596
                     /locus_tag="PLW82_01015"
     CDS             2147..2596
                     /locus_tag="PLW82_01015"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOJ45888.1"
                     /translation="MSFMDKFLKLLGMHDEEQEESENTMGEEEIAFEEIGVTEMLDEP
                     RVSQSTTKTMRMVVLNCININDATQAVNNLKISRPVIVTLKDTTKEESQRIVDFISGA
                     VYALEGELEEISNDTFVFVPKNVVVDKAKCEELVKEDKSALFKDVEE"
     gene            2645..3439
                     /gene="proC"
                     /locus_tag="PLW82_01020"
     CDS             2645..3439
                     /gene="proC"
                     /locus_tag="PLW82_01020"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOJ45889.1"
                     /translation="MGKLGCIGYGAMGSALIAGFVSAGFSTREILVYDTSSTARDKAK
                     QQGFLIADDLGQLEECDYVLLAVKPQQLETVAASWKQERRDQVVISILAGVDTKTLRA
                     ALNREKIIRVMPNLPAQIGRGVIAVHFPGEISDDEKQFVLGLLQKTGKVFEIREELFN
                     AVTALSGSGPAFVLLILEAFAQAGVMEGLDFRTALDMAVETMVGTAEHLKAQKMHPAE
                     LRDMVTSPKGTTAAGLLVLEDEATRASIIKGIRAAAERSRQLQEGK"
     gene            3446..3688
                     /locus_tag="PLW82_01025"
     CDS             3446..3688
                     /locus_tag="PLW82_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOJ45890.1"
                     /translation="MGIIIGLLNYAVSALIWIILIRVILSWIRIDPYGNPVVRLIYEV
                     SEVLLAPVRRLLPPVGFLDFSPFVVILVLELIRRII"
     gene            3702..4490
                     /locus_tag="PLW82_01030"
     CDS             3702..4490
                     /locus_tag="PLW82_01030"
                     /inference="COORDINATES: protein motif:HMM:NF037631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HOJ45891.1"
                     /translation="MRINREALLKVTQDIDEKAVVARYIDLAEQVFADVLPKLGPFLN
                     LREQELCETVINQIEGVALRLDGGFRGAERKRGIVIPDFYGSDPVDAKLSALLIQGKL
                     PRDVSHRDFLGSILGTGIDRSRVGDIILVDGGAQVVVDRDIATYLIQNLAKVGSVNVE
                     VSEIDPEQIIFGEERVKEIKTTVSSLRLDSVAASGFGMSRTAMTREIKAGRVKLNWQE
                     VTSPSRDVSAGDIISIRGRGRVEVAEVGGTTRKGRIHLTLKRFL"
     gene            complement(4665..5321)
                     /locus_tag="PLW82_01035"
     CDS             complement(4665..5321)
                     /locus_tag="PLW82_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOJ45892.1"
                     /translation="MDVIELQELLQTLGIDPEYVDELVQHNLISQPLSESGDEPLFPA
                     AVVGEIQHIQGLKSLGYSLSDIAKILKVTDQKKAAKPQRARFYTVGQVASLLNLTKRT
                     LDFWVEKGVIKPSSTSKSGYRLFSEDAVRVISFVKDLQTIGFTLEQIKSVTDTLNENF
                     TPTGPETASAISLMQQKISDNEKSIKSLKAALKTIQKHARGLKIPHENLEKLDEEENN
                     "
     misc_binding    5463..5688
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5721..>7195
                     /gene="ileS"
                     /locus_tag="PLW82_01040"
     CDS             5721..>7195
                     /gene="ileS"
                     /locus_tag="PLW82_01040"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOJ45893.1"
                     /translation="MDYSKTLQLPANVFPMRANLPEREPQMQKAWDEQDIYFKVLKKN
                     EKGEKFILHDGPPFANGHIHIGHALNKTLKDIIVKYKALRGYYTPYVHGWDTHGLPIE
                     QAMIKEMKLNRHTIDPVEFREKCRDYAHHWIEVQKADMQRLGLWGDWENSYITLTPDY
                     EAVQIGVFGEMFKKGYIYKGLKPVYWCTSCETVLAEAEIEYGDHKSPSIYVAFDVVEG
                     KGSVEAGDAFVIWTTTPWTIPANLAIAVHPDFDYVKVKVGDKNYVVAEGLLENVVTEL
                     GFADYEVLAKFKGSDVEGIRCKHPLHDRDSVVILGTHVTLEGGTGCVHTAPGHGLEDF
                     EVGMEYGLPAFSPVDDRGYFTEEAEQYAGLSLDEGGKQVVKDLSENGHLLKMGWITHQ
                     YPHCWRCHNPIIFRATSQWFASVDGFREEALKAIDEVKWIPGWGKDRIANMVRERRDW
                     CISRQRTWGVPLPVFYCEDCGKELINDDTINAVRELFAKEGS"
BASE COUNT         1901 a         1535 c         1973 g         1786 t
ORIGIN      
        1 aaggctaagg gactacatgt tccgcacagg agagttggta agggatgtag gtacggtttt
       61 ggtgggacgg gaggcagttg aagcaggtct agtgaatgcc gtaggtggcc tcagcgatgc
      121 catagccaaa ctgaacgagc tttgcggaag gaaatccgca cagcagggct tctttgccga
      181 tcagttcaac taccagcccc aacggatgag accgcccgaa tatgggcctc gtcccggcgt
      241 gccgcagccg gtaccacccc agttcaatcc taatccggga ggtctgtaca gctgatgttc
      301 tacacagtcc ttccactaga actcatcatg gaagaggaag aacgaacact ggaatggaag
      361 actgttggga ctgcatcgct tctcgttgag cctttaggag atggcaccta tagactggag
      421 cgcatcgaag gcgcacctct atcctggtac agagactaca atcccggaga cattattaag
      481 ctctaacttg ccctctagag tttttggccg cctaggcggc ctttattatt gaaatggggt
      541 attgttattg tataattaaa tcaaacagat acggatttta ttctcaatta agcgatgtga
      601 gcacagtgaa gaaaaaaact aagggcatac ttttctttgt gattctggca gttattgtcg
      661 ttggtgccgt tatttggctg aagtggatca atcgcagtcg ctacattgaa gctgctactg
      721 acacgctggt tagagcggtt gaagttgatg ccctctgcta caggtttgaa gtgcccttgt
      781 atgcaccggc agacggcaag attgaacagc tggtggccga taacacccgt gttccagcaa
      841 ataccgttgt tgcgatggta ggttcgtatc ccgtctatgc agaacgggct ggcactgtct
      901 cttggcggta cgacggtctg gaaaaactgc tcatacccgg tgtgattatg gtcctgaaac
      961 acgatttttt aggtgactgg gataagccgg taaaaaaggt ctcctctggt gatgtggtgt
     1021 acaagggaga catcgtaggg aagttggtag acaactattc ctggtactta atcgtctact
     1081 ccaacgtaag tgtctacaga aacgaaaaag ttcaggttga aatcggcggc aagatctacg
     1141 aaggagtagt gcaagaggtt ctaagcgaca acagattcag cgtggtcatc aactattatg
     1201 acagcgatgt agcaggttat agagttcttc ggaaaatcaa gctttacaag gaacctctta
     1261 gaggtgtgat cataccacag gatcttttga tcgagaccga tgacggaaca tatgttaaaa
     1321 taagttatcg aaacagggaa cgcacccaac ctgttagggt tgtaggtaag tgggaggaca
     1381 aggtcgtagt ggacggtctg cccaaaggtt ccttagttgt agtttctcct gcaaggtaag
     1441 aggtgtgtaa atggcaatca aagaaaactt acttcgtatt agggaaaaca tcgagaagac
     1501 atgtctgctt tgcggtaggg atcccagaga agtcaagctg cttgcggtat ccaagacctt
     1561 cccgatttcg tcgattttgg aggcatatga agcagggcaa cgggattttg gtgaaagcag
     1621 ggttcaggag tttgtgccga aataccaaca gctccccgac ctcagttggc actttattgg
     1681 acacattcaa aagaacaagc tgaataagct actggacact gaggttttta ttcattcggt
     1741 ggatagtctt gaacttgccg aaaagatcag tgaaaaggcc ttggagcggc agaaggtcgt
     1801 caaagtgctt ctgcaggtaa atatcgacga tgatccttca aagtttggct tcacgtcaga
     1861 aggtcttttg caaaatatag agaagatttt acaattaaag ggaatagccc caacaggact
     1921 tatgactatc ggagctctgc ataaagatcc taaagaaacc agggctagtt ttgcaaagct
     1981 tcgaggatta agggacgctc ttagcgaagt aactaagcac gagtttcccg agctatcgat
     2041 gggaatgagc ggagattatc acgaagcaat acttgaaggg gcaaccattg taagagtcgg
     2101 gacaggggtt ttcggccagc gttagacaaa acggggaggt gttggtatga gctttatgga
     2161 caaatttctg aaactactgg gaatgcacga tgaagaacag gaagaaagcg aaaatacgat
     2221 gggcgaagaa gaaatcgcct ttgaagaaat cggggttaca gagatgcttg atgagccaag
     2281 agtaagccaa tcaacgacga aaaccatgag aatggtagtg cttaactgca taaacatcaa
     2341 cgatgcaacg caggctgtga acaacctgaa gatcagccgg cccgtgatcg ttacgttaaa
     2401 agataccact aaagaagaaa gccagcgtat tgtagatttc atttccggtg ccgtgtacgc
     2461 gcttgaaggc gaacttgaag aaatctcaaa cgatacgttt gttttcgttc ctaaaaacgt
     2521 tgtggtggac aaggccaaat gcgaagagtt ggtgaaagaa gacaagtcgg ctttgttcaa
     2581 ggacgtggag gagtaatagg aaaggcaggt gggacaggcg ctcttgcggc gcctgctttg
     2641 attaatgggg aaactaggct gtattggtta cggagccatg ggcagcgctc ttatagctgg
     2701 atttgtgagc gctgggtttt ccacaagaga gattttggtc tacgatacaa gcagcactgc
     2761 gcgggataag gccaaacagc agggctttct gatagcagac gatctcggtc agctggaaga
     2821 gtgcgattat gttcttcttg cggtaaaacc gcagcaatta gagacagttg ctgcaagctg
     2881 gaagcaggaa agacgcgatc aagtagtcat ttccatactt gcaggtgtgg ataccaagac
     2941 gttgagagcg gctttaaaca gagagaaaat aataagggtc atgccaaacc ttcctgcaca
     3001 aatcggtaga ggagtcattg cggtccactt tcctggcgag atatcagacg acgaaaagca
     3061 gttcgttctg ggcctcctgc agaagaccgg aaaggttttc gagataaggg aggaactgtt
     3121 caacgctgtt accgcactga gcggctccgg accggctttt gttcttctca ttcttgaagc
     3181 ttttgcacag gccggtgtta tggaaggact ggatttccgt acagcactgg acatggcagt
     3241 tgagaccatg gttggtacgg cagaacacct gaaggcacaa aagatgcatc ccgcagaact
     3301 tagggatatg gttacatcgc ccaaaggaac caccgcagct ggtcttttgg ttttagaaga
     3361 tgaagccact agggcatcta ttattaaagg gatcagagct gctgcagaaa gaagcaggca
     3421 gcttcaggag gggaagtaga ggtccatggg cattattata ggactactca attacgcagt
     3481 ctctgcattg atttggatta ttctgattag ggtaatcctc agctggatta ggatcgaccc
     3541 gtacggcaat cctgttgtaa ggttgatcta cgaggtcagc gaggtgttat tggcaccggt
     3601 gcggaggtta cttcctccgg tgggatttct cgacttttct ccgtttgttg taatcctcgt
     3661 actagaactt attagacgaa ttatctaagg caggcgattt tgtgcgcatc aatcgagaag
     3721 ctttgcttaa agtaactcaa gacattgacg aaaaagctgt tgtagctagg tatattgatc
     3781 ttgcggagca ggtttttgca gatgtactgc caaagctcgg tccgttcttg aatcttcgcg
     3841 agcaagaact ttgcgaaact gtcattaacc aaatcgaagg ggtagcgctc cgcctggacg
     3901 gtgggttcag aggagctgaa aggaagagag gcatcgtcat tccggacttt tacggatcgg
     3961 accctgttga tgccaagctg tcagccctgc tcatccaggg caagttgccc cgcgatgtga
     4021 gccacagaga ctttttaggt tccattttgg gaacgggaat cgaccgtagt agggttggcg
     4081 atatcatcct tgtggatggt ggcgcgcagg tggttgtgga cagagatata gccacttatt
     4141 tgatccaaaa cctggcaaag gtcggctcgg taaacgttga agtcagcgaa atcgatccgg
     4201 aacagatcat ctttggcgaa gagagagtga aagagatcaa aaccacagtg tcatctctaa
     4261 gactcgattc tgtggctgca agcggctttg gaatgtccag gacggccatg accagggaga
     4321 ttaaggccgg gcgggttaag ctcaactggc aggaagtaac atctcccagt cgggacgttt
     4381 ctgccggaga tattatctcc ataaggggaa gaggccgagt ggaagttgca gaagttggcg
     4441 gaaccaccag gaaaggcaga attcatctga cccttaagcg ctttttgtag gcaatggctg
     4501 gtggcctttg tctcttccgg aaagacgcgc agcctttctt cggaaccttt ggcagtggtt
     4561 aacagaacag ttaatgaaca aaaaaggagc agtgctcgct acttggtagc tctgcagtgc
     4621 tccttttctt gtatctgtct ttttttggaa gatccgcgta ttgctcagtt gttctcttct
     4681 tcatctagtt tttccaggtt ttcgtggggg atcttcaatc cacgtgcgtg tttttggatg
     4741 gtcttcaatg ctgctttcag cgacttgatg gacttctcgt tgtcgctgat tttctgctgc
     4801 ataagggaaa tggcggaggc tgtctccgga cctgtggggg taaaattctc gtttaaggta
     4861 tcggtaacgg attttatttg ctctaaggtg aatcctattg tctgtaggtc tttaacaaaa
     4921 ctgattaccc tgacggcgtc ttcgctgaac aagcggtaac cgcttttcga tgtggagctg
     4981 ggcttaatga cgcctttttc cacccaaaag tccagcgttc ttttggtcag gttcagcaag
     5041 ctagccactt gacctacggt atagaaacgg gctctctgcg gctttgcagc ttttttctgg
     5101 tctgtcactt tcagaatttt agcgatatca gagaggctgt aaccaaggct cttcagtcct
     5161 tgaatgtgct ggatttcccc gacaactgca gcgggaaaca gaggctcgtc cccgctttct
     5221 gaaagtggct gagagatcag attgtgctgc accaactcgt caacatactc ggggtctatg
     5281 ccgagggtct ggagcaattc ttgtagttcg ataacgtcca tgtgtaccca cctttactga
     5341 aacgcatacg ttacgctttc attataggcg ttcgctgttt ggtgtcaatt gtcctgcgaa
     5401 aattgaccac actccacttg attcgggttg aagaaaccct tataatagtt aataatattt
     5461 tctgcaaatc gatgaaggga acgagtagtc ggcgaaagct gtaagagagc cggggttagg
     5521 tgcgagcccg gtcagcagaa gttgatgaaa atcatccctg agtgtacggt tgaagtgaaa
     5581 gtaagccgtg ccggctgcca ccgttaaccg gccaagagag gacctgtagc atacgataca
     5641 ggtcaacttg ggtggtaccg cgggtagaaa tcttttcccg tcccttgtct gggacgggat
     5701 tttttttgga ggtaggataa atggattaca gtaaaaccct acagcttccc gctaacgtgt
     5761 ttcccatgag ggcaaatctg cctgaacggg aaccgcagat gcaaaaggcg tgggacgaac
     5821 aagatatcta tttcaaagtg ttaaagaaga acgaaaaggg cgagaagttc atcctccacg
     5881 atggccctcc atttgctaac ggccacattc acatcggcca tgcgctgaac aagactctta
     5941 aggacatcat cgtaaagtac aaagctttga gaggatacta cactccctac gttcacggct
     6001 gggatactca cggtctgcct atcgaacagg caatgatcaa ggagatgaag ctgaacaggc
     6061 acaccatcga tcctgttgag tttagggaga agtgccgtga ctacgcccat cactggattg
     6121 aagtgcaaaa agccgatatg cagaggctcg ggctctgggg agactgggag aactcctaca
     6181 tcactctcac tccggactat gaggcagtgc agatcggtgt attcggcgag atgttcaaga
     6241 agggatatat ctacaaaggt ctgaagccgg tctactggtg tacaagctgc gaaactgtac
     6301 tagcagaagc ggagatcgag tacggcgatc acaagtctcc ttcgatttac gtagcgtttg
     6361 atgtagttga gggcaagggt tctgtggaag caggagatgc ctttgtgatc tggactacca
     6421 caccctggac cattcctgca aacctggcca ttgccgtgca cccagacttt gactacgtca
     6481 aggtcaaagt cggtgataag aactacgttg ttgcagaagg cctcctggaa aatgtcgtaa
     6541 ccgaactggg ttttgcagac tacgaggttc ttgccaagtt caagggcagc gacgtggaag
     6601 gcatcaggtg caaacatcct ctccatgatc gtgactctgt ggtgatcctg ggaacccacg
     6661 ttacgctgga aggcggtacg gggtgcgtcc acaccgctcc tggacacggt cttgaggact
     6721 tcgaagtagg tatggagtac ggcttgcctg cattttcacc agttgatgat cgcggctact
     6781 tcaccgaaga ggcagagcag tacgcaggac tcagccttga tgaaggcggc aagcaggttg
     6841 taaaggacct ttccgaaaac ggtcatctcc tgaagatggg ctggatcact caccagtatc
     6901 cgcactgctg gaggtgccac aacccgatta tcttcagagc gaccagtcag tggtttgctt
     6961 cagttgatgg gtttagagaa gaagcgttga aggcaatcga tgaggttaaa tggatccctg
     7021 gttggggcaa ggaccgcatc gctaacatgg tcagagaaag acgcgactgg tgcatttccc
     7081 gtcagagaac ctggggcgta cctctgccgg tgttctactg tgaagactgc ggcaaagaac
     7141 tgatcaacga cgacaccatc aatgccgttc gtgaactctt cgctaaagaa ggttc
//
LOCUS       DAOSDA010000023         7195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_6669_length_7195_cov_2.956723, whole genome shotgun sequence.
ACCESSION   DAOSDA010000023 DAOSDA010000000
VERSION     DAOSDA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6669_length_7195_cov_2.956723"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PLW82_01045"
     CDS             complement(<1..736)
                     /locus_tag="PLW82_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ45894.1"
                     /translation="MKRKRILTLGLVVALAAFTFASSVEIAGTIDETNNIWGPEGSGA
                     LIAAETIVLTGDIVVPQGITLTIMPGARIVAQNVQNPNLSQNNLFSGLEIAVHGKVAA
                     FGTKEQPISFSGKLSEPGSWQGFFTLGSLTLQNVIITDAECAVTASDGSLQISSCTFR
                     GNNTAVRLWGWQNAKIQQSLIENNIDGISCSSTPATIQGNTIKDNNVGIRILLGESVP
                     VITENTFLSNREYHIYNLSVLDVKAYP"
     gene            complement(733..2262)
                     /locus_tag="PLW82_01050"
     CDS             complement(733..2262)
                     /locus_tag="PLW82_01050"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ45895.1"
                     /translation="MLVLLLALLLALPVSGYTDRPYYYLSEIKNTTDITYPVAIRQGA
                     KPPKQLSDGEVEKLVHEAMSLADFDSLLTEDVKTIMLKPNIVEPFESGSGVITDYRVV
                     QAAAVYIHERRPDIKIIVGEGAGGWVPAGYEWGAFPGAKAEDGFAVAGYRDMVAYLKK
                     KYPDLDIEIIDINIPAEDIVEVVPKVPFADDKYYIHRMVRDADLLVTIPVLKIIETCQ
                     VTLGLKNNVGIAAGVVYGWAKMRGFPYTKNAGGLRHTYPVIDEEIADLVQARVPDFVI
                     IDGIVGMEQDKTHIRRGIKKQANMIIAGRDPVAVDAAASRVMMLNPRDVEYITLCYYI
                     GLGSLEPIIVGDPLEEVAERWIKPDTRYSPRGHFGQGRRIFEVAGDSGEFSEPILFMD
                     TLINPWVLGDARRYTLRTGFELEETMDLELWVGSDCSMTVYLDGEPIYRHSGRRKHEL
                     PNDIVKLANVQAGTHEVEIILGQKGSFSLTIGEGLPRFMAGRDQFDGTTPLGLKWRWE
                     D"
     gene            complement(2280..3194)
                     /locus_tag="PLW82_01055"
     CDS             complement(2280..3194)
                     /locus_tag="PLW82_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOJ45896.1"
                     /translation="MYESRSTLFDYIAYYIDVYSEYPAFRVVLVFFDLFWQYLPYIIA
                     SILVSSLIARYLRPEKLQKTFTYNRFWSILLASLLGTISPLSSYALIPFAGSLLLLGF
                     PLGPVSAFIFSTPLMNPTIFILTLRGLGLKMALARTISTILIGIASGYLSEMIFRHVS
                     VLKEDPKFKLPKTKAFLPYVLSMAKYMSKAFFIALFLASLVRVFLKPEQVAGLLGPDK
                     PWSISVAALLGVPFYTCGGATIPVVSSLIDLGMSHSAGLAFFIAGPATNLPNVSAVLL
                     TFKWQYLVFYLLLTLLLAIGCGYVFSIF"
     gene            complement(3245..3523)
                     /locus_tag="PLW82_01060"
     CDS             complement(3245..3523)
                     /locus_tag="PLW82_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45897.1"
                     /translation="MDVKFYPQKPEAYRPLTIIYQGRIGDNAKIMRIHGGYGSTNWEE
                     VFDLPMSLWPDNGWTINIAVLPHKLLNIAFHDGEGNWEEPIQLPIISP"
     gene            3615..4601
                     /locus_tag="PLW82_01065"
     CDS             3615..4601
                     /locus_tag="PLW82_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ45898.1"
                     /translation="MVFKIKTFIKDLTVGQKIDDIFVIKGKQMVPYSSKPNEYYLKFT
                     LADASGEASGRIWQGVFSLNEVIEDGYPYRIRGSVVEFKGALQVNVDDLAKVAPEDVR
                     AEDFIAAGPYSTKDLDRRLTDLIESLPKSSGLTLLRKIFGDEKIRSSFLLWPAATSVH
                     HNWLGGLCQHSLEVAELCQFLARTYNLSMSLVTVGALLHDIGKLREYQFTGAAFTLTD
                     EGKLNGHMQLGLEMILPYLSGLDPIEAASIKHIILAHHGKKEWGSPVVPQTPEAYAVF
                     LADYTSSKLVRIKSVLEGVTSGWSDYDRFLETSLFSGQSDRGDLGSLFDDFE"
     gene            4747..5763
                     /locus_tag="PLW82_01070"
     CDS             4747..5763
                     /locus_tag="PLW82_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ45899.1"
                     /translation="MAKKIKIGVIGAGAIAEHAHLAGYNLIPDLCEITAICDINKERA
                     EEMAKKFNIPEVYTDYKEMLAKADIDAVSICTPNALHAPMTIDACNAGKHVLCEKPMA
                     VTVEECEEMIKAAEANNVQLMVGFTHRFYNYNQKAKELLDSGVIGKPFMARVRFAHEG
                     PYISWPAKSDWFFNKEMAGGGALLDMGVHALDILRFMIGEIKDISANVKTLVRDIEVE
                     DNAVCAVTFENGAMGYLEVGWSSKAGRLGIEIYGDDGSMVIDYETPIRVFIDKKKDIG
                     GPGWVDLTEYKGGGWDTEMKAFVEALIEGKPVPVDGHDGLVGVKLATAAYKSNAEGVR
                     VEVK"
     gene            complement(5827..>7195)
                     /locus_tag="PLW82_01075"
     CDS             complement(5827..>7195)
                     /locus_tag="PLW82_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ45900.1"
                     /translation="ITAEGTADQPIIFTSIYDDAHGGDTNKDGNLSKPQRGDWNRILL
                     SYARDSSFKYCHFYYGGGDSSYDCTVEVEEDKATFENCVFANNKGSQRGALFIEAADS
                     RTSVQSCVFYANEKPLVMAAGMSIDDSNTFHNPTKPTEKNEQNGIFISRFYLHIDGNV
                     TWGETSVPFVIPGQLDVTSGAVLNLDPGVALKFTKDGQIHVYQGIIKARGTAANPICF
                     TSIDDDSVLGDTLGDGAITPKPGDWKSVEVYDSPAFANEFVYCHFAYGGGNSSDDGTL
                     IIRYASAVIENCTFTSCNSSRTGALNLEKANPMTLATCTFQNNVIPVLIGGEISTENS
                     NTFTGNTKNGIFVSENANTVEGDISWLDTSVPYVVQGDFYIQERSTLTIGDGIIVKAF
                     PGIEICMDGDNIVRLGDATFTAYADDTRGGNTDGVSGTPLPWNGIYDYATGEYVSWPE
                     MYYVK"
BASE COUNT         1762 a         1743 c         1935 g         1755 t
ORIGIN      
        1 tgggataggc tttcacatct aggacgctga ggttgtagat gtggtactcc ctgttgctta
       61 aaaaggtgtt ttcggtgatc acgggaaccg attcgcccaa gaggatgcgg atgccaacgt
      121 tgttgtcttt gatggtgttg ccctggattg tagcaggagt cgaggagcag gagatgccgt
      181 cgatattgtt ttcgatgagg ctctgttgga ttttggcgtt ttgccaaccc caaagcctta
      241 cagcagtgtt gttccctcta aaagtgcaac tggagatctg cagggatccg tctgatgcag
      301 tcactgcaca ctctgcatcg gtgatgatca cattctgtag tgttaggctg ccaagggtga
      361 agaaaccctg ccacgaaccg ggttcagaga gctttccgga aaaggaaatg ggttgttcct
      421 tggttccgaa ggctgcgact ttgccgtgga ctgctatctc cagaccgctg aagaggttgt
      481 tctgggagag gttggggttt tggacgtttt gggctacaat tctggctccg ggcataatgg
      541 tgagggtgat cccctgaggc accacgatat ctccagtgag aacgatggtt tctgcggcaa
      601 tcagagcacc tgagccttca ggcccccaga tgttgttcgt ttcgtcgatg gtgccagcga
      661 tttccaccga gcttgcaaag gtgaaagcgg caagtgctac aacaagaccg agagttaaga
      721 tgcgttttct tttcaatctt cccatctcca tttcaagccc agtggggtag tgccgtcaaa
      781 ctggtctctt cctgccatga accggggcag tccctctccg atagtcaggg agaacgatcc
      841 cttctgcccc aagattatct caacttcgtg ggttcccgct tggacattag cgagcttgac
      901 tatatcgttt ggcagttcgt gcttgcgcct gcccgaatgt ctgtagatgg gttcaccgtc
      961 aaggtaaacg gtcattgagc agtcgctgcc aacccagagt tccaggtcca tagtttcctc
     1021 caactcaaaa ccggttctga gggtgtacct cctcgcatcg cctagcaccc aggggtttat
     1081 gagggtgtcc ataaagagga taggttcgga aaactcgccg ctgtctccgg caacctcaaa
     1141 gatcctccga ccctgtccga agtgcccgcg aggcgagtag cgggtgtcgg gtttgatcca
     1201 cctttcggcg acctcttcca gaggatcgcc aacgatgatc ggctcaagag aacccagtcc
     1261 gatgtagtag cagagggtaa tgtactcaac atccctcggg tttaacatca tgacgcggga
     1321 ggctgcagca tcaacggcaa ccggatctct accggcaatg atcatgttgg cttgcttttt
     1381 gatgccccgt ctgatgtggg ttttatcctg ttccataccg acgatgccgt cgatgattac
     1441 aaagtcgggg actcttgcct gtaccaggtc ggcgatttcc tcatcgatca ccgggtatgt
     1501 gtgcctgagg ccaccggcgt tttttgtgta ggggaaaccc cgcatctttg cccaaccgta
     1561 gaccacacct gcggcaatgc ccacgttgtt ttttagacct agagtgacct gacaggtttc
     1621 aatgatcttc agcactggga tggtgaccaa aagatcggcg tcccttacca ttcggtggat
     1681 gtagtacttg tcgtctgcga aaggtacttt gggcacaacc tcgacgatat cctctgcagg
     1741 tatgttgatg tcgatgatct cgatgtccag atcggggtac ttcttcttca ggtaggcgac
     1801 catgtccctg tagccggcga cggcaaaacc gtcctcagct ttggcaccgg ggaaggcgcc
     1861 ccattcatag cctgcaggaa cccagccccc tgctccttca ccgacaatga ttttgatgtc
     1921 cggtctccgc tcgtggatgt agacagcggc ggcttgaact acccgatagt cggtgatcac
     1981 gcctgaaccg ctttcaaaag gttccacgat gtttggcttc aacataatgg tcttaacgtc
     2041 ttctgtcagg aggctgtcga aatctgcaag gctcattgcc tcgtgaacta gcttttctac
     2101 ttcgccgtcg gaaagctgtt tcggtggctt tgccccctgt cggatagcta ccggataggt
     2161 gatgtctgtc gtgtttttaa tctcacttaa gtagtaataa ggcctgtcgg tatacccgga
     2221 gacgggcagc gcaaggagaa gagcaagcag cagaactaac atttaaatcc ccccaaaatt
     2281 caaaaaatgg aaaagacgta accgcaccca atagcgagaa gcagcgtgag caagaggtaa
     2341 aataccaaat actgccattt gaacgtgaga agcacggcgc tgacgttggg caagttagtt
     2401 gcaggtcccg caatgaaaaa ggccagaccg gcactgtggg acatccccaa gtcgataaga
     2461 gagcttacca cgggaatggt ggcaccaccg caggtgtaaa aaggtacgcc caaaagagct
     2521 gcaacagaga tgctccaggg cttgtccgga ccgaggagac cggccacctg ctcgggtttg
     2581 aggaagaccc tgaccaaact tgccagaaag agagctataa agaaggcttt gctcatgtac
     2641 ttggccatgg aaaggacgta ggggagaaag gctttggtct tcggcagctt gaacttaggg
     2701 tcttctttaa gaaccgaaac gtgccgaaag atcatttcgc tcaagtagcc gctggcgata
     2761 ccgattaaga tggtagagat ggtcctggca agggccatct tcagcccaag accgcgcaag
     2821 gtgagaataa agatggttgg gttcatcagc ggcgtactga agataaatgc agaaaccggt
     2881 cctaagggaa accccagaag cagtagcgag ccagcaaatg gtatcagggc gtagctggaa
     2941 agaggggata tggttcctaa tagggaagcc aagaggatgc tccaaaacct attgtaggtg
     3001 aaggtttttt gcagtttttc tggccttagg tacctcgcta tcaagctgct gaccaggatg
     3061 ctcgcgataa tgtagggaag atactgccag aatagatcga agaacaccaa aaccactcta
     3121 aaggctggat actcgctgta aacgtcgatg tagtaggcta tatagtcgaa caaggtggat
     3181 ctgctctcgt acatcgtaac cctcctttta ctaaagggtt accgttcaac aagaaagttc
     3241 ctccttacgg agaaataatc ggcagctgga ttggctcctc ccagttgcct tcgccgtcgt
     3301 gaaaggctat attcagaagt ttgtggggga gaactgcaat gttaatagtc cagccgttgt
     3361 cgggccaaag gctcatagga agatcgaaaa cctcttccca attggtcgaa ccgtatcctc
     3421 cgtgaatgcg cataatcttg gcattgtcgc cgattctgcc ctgatagata atggtcagcg
     3481 gcctgtaggc ctcgggtttt tggggataaa acttgacatc cataaaaaac acctccggtc
     3541 atagtctttc caaaatcgtt tgagatagac gtcaaaaata gagtatactg gaagaagaga
     3601 ctacttttgg aaaggtggtt ttcaagatca agaccttcat caaagatctg actgtaggtc
     3661 agaagattga cgatatattt gtgatcaaag ggaagcagat ggttccctat agcagcaaac
     3721 ccaatgagta ctacctgaag ttcactctag ccgatgcctc aggcgaggcc agtggcagaa
     3781 tctggcaggg agttttctct ctaaacgaag tcatcgaaga cggctatccg tacaggataa
     3841 ggggcagtgt ggtagagttt aagggcgccc tgcaggtaaa cgtggacgac ctagcaaagg
     3901 tcgcgccaga agacgtgaga gccgaagact tcatcgccgc cggtccctac tccaccaaag
     3961 atctggacag gcgcctcacc gacctcatcg aaagcctgcc taaaagctcg ggacttacgc
     4021 tgcttagaaa gatctttggc gacgaaaaaa ttcgctcgtc gtttctgctc tggcctgcag
     4081 caacttcggt gcaccacaac tggctgggtg gtctctgcca gcacagcctg gaggttgcag
     4141 agctctgcca gttcctggcc aggacctaca atctgtcgat gtctttggtg acagtgggtg
     4201 cccttttgca cgatatcggt aaactccgtg agtaccagtt taccggtgct gcctttaccc
     4261 taaccgacga aggcaagctc aacgggcata tgcagctcgg cttagagatg atcctgccat
     4321 atctctccgg gctcgatcca attgaggcag ccagcatcaa gcacatcatt ctcgcccacc
     4381 acggcaaaaa ggagtggggt tcgccggtag ttccccagac tccagaggct tacgccgtgt
     4441 ttcttgcaga ctacacctca tcgaagctgg ttagaataaa gtctgttctc gaaggggtaa
     4501 cctcaggttg gtccgactac gacaggttcc tggagaccag cctcttttca ggtcagagcg
     4561 acagaggcga tttaggcagc ctttttgacg actttgagta agactgttag gagttttgcc
     4621 tcaaaaaaga ccaggtcaaa cccgtttgag gggcttggcc aataaacaca agggtatgag
     4681 ttctagagaa gggaagaccc ttcgtttcta gaataaatta gtagaaacat caaggaggta
     4741 agaattatgg cgaagaaaat caaaatcggc gtaattggcg ctggagccat cgctgaacac
     4801 gcacatttag caggttacaa cttgatccca gatctctgcg agataacagc tatatgtgat
     4861 atcaataagg aacgggccga agagatggcc aagaagttca atataccaga ggtctacacc
     4921 gactacaaag agatgctggc caaagccgac atcgatgcag tatccatctg tactccaaac
     4981 gcactgcacg ctcctatgac catcgacgca tgtaacgcag gcaaacacgt tctctgcgag
     5041 aagccgatgg cggtaaccgt tgaagagtgc gaagaaatga ttaaagccgc tgaagctaac
     5101 aacgttcagc tcatggttgg cttcacccac agattctaca actacaacca gaaagccaag
     5161 gaactcctcg actccggagt tatcggcaag ccattcatgg ccagagtccg gtttgctcac
     5221 gagggtccat acatttcctg gcctgcaaag agcgattggt tcttcaataa ggaaatggca
     5281 ggcggcggcg ctctcttgga tatgggtgta cacgcactgg acattctccg cttcatgatc
     5341 ggtgagatta aggacatctc tgccaacgtc aagaccttgg tccgcgacat cgaagttgaa
     5401 gacaacgcag tgtgtgctgt taccttcgaa aacggcgcca tgggctactt agaggtagga
     5461 tggtcctcca aggccggtcg tttgggtatc gaaatctacg gtgacgacgg ttccatggtc
     5521 atcgactacg agacaccaat tagagtgttc atcgacaaga agaaggacat cggcggccca
     5581 ggttgggttg atctcaccga gtacaaaggc ggcggctggg atactgagat gaaggcattc
     5641 gttgaggctc tcatcgaagg caaaccagtg cctgtagacg gacacgatgg tctggttggc
     5701 gtaaagctgg ctactgcagc ttacaagtcc aacgcagaag gcgtgagagt agaggttaag
     5761 tagtttccgc aaacgtaaac aaagcaccct cgactgaggg tgctcttttt tgttatggca
     5821 aagtgcctac ttaacgtagt acatctccgg ccaggatacg tattcgcctg tagcgtagtc
     5881 gtaaatgccg ttccaaggca gaggagtacc ggatactccg tcagtatttc ctccgcgggt
     5941 atcgtcagcg taggctgtga aggttgcatc gccgagtctg acgatgttat cgccatccat
     6001 gcagatctca ataccgggga aggccttcac tataatgccg tctccaatag tgagcgttga
     6061 cctttcttga atgtagaagt cgccctgcac cacataaggt acgctggtgt ccagccacga
     6121 gatatcgccc tcaacggtgt tggcgttctc tgaaacgaag atgccgtttt tggtgttgcc
     6181 agtgaaggtg ttggagtttt ctgtgctgat ctctccgccg atgaggacgg gaatcacgtt
     6241 gttttggaaa gtgcacgttg ccaaagtcat tgggttagcc ttttccaggt ttaaagcacc
     6301 ggtccgtgaa ctgttgcaag aagtaaacgt gcagttttct atcaccgcag aagcgtagcg
     6361 gatgatcagt gttccatcgt ctgaggagtt tcctcctccg taggcgaagt gacagtagac
     6421 gaactcgttg gcaaacgctg gggagtcgta gacttcaacg cttttccagt ctccaggttt
     6481 gggagttatg gccccgtcgc ccagggtgtc tcccaggacg ctatcgtcgt cgatggatgt
     6541 aaaacagatg gggtttgctg cagttcctct ggctttgata attccttggt aaacgtggat
     6601 ttgtccgtct tttgtgaatt ttaaagctac accaggatcc agattcagca cagctccgct
     6661 tgtcacgtct agttgtccag ggataacaaa cggtacggaa gtctctcccc aagtaacatt
     6721 tccatcgatg tgaaggtaaa accttgagat gaaaattccg ttctgctcgt tcttctcggt
     6781 gggttttgtg ggattgtgga aggtgttgga gtcgtcgatg gacatgcctg cagccatcac
     6841 cagcggtttt tcgttggcat agaagacgca cgactgtact gaagtacgag aatctgcggc
     6901 ttcaatgaaa agagctcctc tctggcttcc tttattgttt gcgaacacgc agttttcaaa
     6961 ggtagcctta tcctcttcta cttccaccgt gcagtcgtag ctggaatctc cgccgccgta
     7021 gtagaagtgg cagtacttga aactggagtc tctagcgtag ctgagcagta ttctgttcca
     7081 atcaccgcgt tgaggttttg aaaggtttcc gtccttgttg gtgtcgccac cgtgggcatc
     7141 atcatagatt gaggtgaaga tgatgggttg gtctgcggtt ccttcggcag ttatt
//
LOCUS       DAOSDA010000024         7023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_6902_length_7023_cov_3.259472, whole genome shotgun sequence.
ACCESSION   DAOSDA010000024 DAOSDA010000000
VERSION     DAOSDA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6902_length_7023_cov_3.259472"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLW82_01080"
     CDS             complement(<1..742)
                     /locus_tag="PLW82_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ45901.1"
                     /translation="MTESIYYDVIITGGSTGGVAAALALLDYGRRVLITEETDWLGGQ
                     LTSQGVSAPDENQYIEYFGAPATYYAFREGVRNYYRKNFRLSDSAKYVARLNPGNCWV
                     SRIGYEPKVGVEVINQMLKKYLENGQLTVLYNAVPVEAEVENDYIKSVTYKLKDGSTK
                     TFSANLYIDASEIGDLLPVVGAEYVVGAEAKSETGEVHAPDTPQPKWVQSYTFPFVIE
                     YRPGENHVIPKPDNYEENKVKQPYTLDVD"
     gene            complement(786..1379)
                     /locus_tag="PLW82_01085"
     CDS             complement(786..1379)
                     /locus_tag="PLW82_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOJ45902.1"
                     /translation="MIWTLIVVLFAGVLAWTFKPKKEKVNVGDLWNDPSIFGPSVLTA
                     EQLDNYVKRINPNAPSLGKYYIEWGKYYGIRGDVAFAQAIHETGWFRFGGDVLPEQNN
                     YAGIGAVGGGARGAYFKTPEEGVIAQLQHLYAYASTEPLPGTQPNVDPRFHLVRRGSA
                     PTWPDLDGKWAVPGVGYGNRVLKIWRDMVVHYGLYRP"
     gene            complement(1473..2789)
                     /locus_tag="PLW82_01090"
     CDS             complement(1473..2789)
                     /locus_tag="PLW82_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOJ45903.1"
                     /translation="MANRLLTIALILLGVLVFSGCDFVKKPGTLIIKTDHEDLDVLLE
                     TANGQEVARGATTHSLDPGTYRVKAFANGYKTLSRTVSVSRGQTAIVNIDLEPLTKKG
                     YLSIDYDLPDGAIFLDDAFYGFTRAGAKPAIEVSPGQYNLKLTRRTFVYEEVITIKEN
                     EVTSVDVPDTITVKVVIDRDKGEVFYWHDKNPANLDYPVPKSVGEITKTTLLEFSEEG
                     IYWFYAKSPGRVFTAEMVVLSKEKLEVEVNLTSAPVEQVRKALASPLLGRTFLAPWEL
                     EDFVRYQKKANPHQIYIAKYYLFFGNELVISGDLAYAQSMHETAYFKFGGDVLPEQNN
                     YAGIGAVGGGARGHSFNTPEEGVLGQFQHLWAYATAKSAGEPVPVRKYSAEPVFWTAN
                     VDPRYHLVTRAKAPTLSLLAGSWATDQQYAHKILKIHNDLLDYLGY"
     gene            complement(2818..3585)
                     /locus_tag="PLW82_01095"
     CDS             complement(2818..3585)
                     /locus_tag="PLW82_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ45904.1"
                     /translation="MSQVGKPLRIAFLSDTHFVEADDFNYKQWQMALEKAKSTDPDIY
                     LVGGDITNSGSKKEAELFFASIKDINKPVLFVPGNHDIGNKVFEGVEGTITSERIAYY
                     RENFGPDFYAENHHGYQFIMLNSQLFQSGLPEEKEQMSFLKKQLEVEGPKIILIHNPL
                     FLNCVDEPGGNYWNAEPEVRKEILDLLAGKGVVAVLSGHIHKLIVNCTDILHLSCPPT
                     SFGVPEIHLGQGFVVLDVTPSNIHFQLYHVESLYEKP"
     gene            complement(4069..5217)
                     /gene="dinB"
                     /locus_tag="PLW82_01100"
     CDS             complement(4069..5217)
                     /gene="dinB"
                     /locus_tag="PLW82_01100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOJ45905.1"
                     /translation="MNRIIVHVDMDAFFAAIEIRDNPELRGKPVIIGGDPNSPRGVVS
                     TCSYEARKFGVHSAQPIAQAKRLCPHGIFLRGNMEKYAAVSRELMQIFDEFTPLVEPL
                     SIDEAFLDMTGCEHFYKGLFDMGSQIKGRIKERLDLTASVGIAHNKFLAKLASDKEKP
                     DGLTIVDDPITFLDTIPLRKMWGVGPSTYKKLESMGITDVPTLRNTPVDTLVRALGKQ
                     GFQLHKLSLGIDNRQVEPPHEAKSIGNEETFPEDISVSQVHKELLYLTDKAARRLRRQ
                     NLKCRTVTVKMRLPDFTTFEKSSTLPEPTADTDIIFQEVKKLAKPYKGPFRLLGVSLS
                     NLAAETSLSLFEEEQNKSKLWEVIDSLKDKGLDIKKAAILEDDPEAGK"
     gene            5253..6410
                     /locus_tag="PLW82_01105"
     CDS             5253..6410
                     /locus_tag="PLW82_01105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOJ45906.1"
                     /translation="MLLVFLKSNREAKVLNGYPNIYRDEIRKVEGVEEDGDICNVFSE
                     DFKFLGKGFYSKSNVSVRMLTLADEEIDEDFFRNRVEKAAQNRRGFGDSYRLIHGEAD
                     LLPGVIADKYRDFVVLQIRNVGMERYKPVLVSALAQVVSPKGIYERSDFLSSSSDILT
                     RNVGLLYGEEPPDELNITEHDLIYKVDIKTGQKTGFFFDQKDSRLFVRSITPNGGLAL
                     DAFSYTGGFALNMAKAGASQVIALDKDEDAVRLGRINAAANGLDNVEFINVRYEDYMQ
                     SYTGEPFDIIVLDPPSIIKKKEERKQGVEIFRNIVSLSVPHLKEEGVLGLCSCAYQID
                     LDLLIEAVRRSYSGSGKTLQVLGVTYQSGDHPWVLSYPESLYLKCLWVKVF"
     gene            6676..>7023
                     /locus_tag="PLW82_01110"
     CDS             6676..>7023
                     /locus_tag="PLW82_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45907.1"
                     /translation="MQVTIPFLQDYPIDVVLMALKPDGTTVVEPMVKKGTEFQAVMTI
                     DPGIWSLSVDLTYQGYILNSIVLAPEVLVEKDTKKLRFDLPDVVATDELFGEISVPDL
                     VKSGEKVTIKTTGT"
BASE COUNT         1699 a         1629 c         1781 g         1914 t
ORIGIN      
        1 aatctacgtc aagagtatag ggttgcttca ctttgttctc ttcgtagttg tcgggtttcg
       61 gtatgacgtg gttttcacca ggcctgtatt cgatgacaaa cgggaaggtg taagactgca
      121 cccattttgg ttggggagtg tcgggagcgt gtacctctcc tgtctctgat ttagcttctg
      181 cacctacaac atactctgca ccgactaccg gaagaagatc gccgatctca ctggcgtcga
      241 tgtaaagatt ggcgctgaag gtcttagtac ttccatcttt tagcttgtag gttactgact
      301 tgatgtagtc gttctcaact tcagcttcta caggtacggc gttgtataga acggtcaact
      361 gtccgttttc caggtatttt ttcaacatct ggttgataac ctcaacgcct acctttggct
      421 cataaccgat tctgctaacc cagcagtttc cgggattgag gcgggcgacg tatttcgcag
      481 agtcgctgag ccggaagttc ttgcggtagt agttacgcac gccttcccta aaggcgtagt
      541 aagttgccgg tgcaccgaag tactcaatgt actgattttc gtcgggagca gacacaccct
      601 ggctggtgag ctgtccgccc aaccaatcgg tctcttctgt gattagtacg cgtctgccgt
      661 aatctaacag cgccagtgca gctgcaacac cacctgtact gccacctgtg attattacat
      721 cgtaataaat gctttctgtc atgcgtgaca ctcctatatg agatattgat agaacagatt
      781 catgattaag gtctgtagag gccgtagtgg acgaccatgt cgcgccagat cttcaggact
      841 ctgttgccgt atcctactcc aggaaccgcc cacttgccat cgaggtcggg ccaggtgggg
      901 gctgaacccc gtcttaccag gtgaaagcgg ggatcgacgt tgggctgggt tccagggagt
      961 ggctccgtgg aagcataggc atataaatgc tggagctgcg caattacccc ttcttctgga
     1021 gttttgaagt aggcgcctct agcacctcca cctactgccc cgattccagc gtagttgttc
     1081 tgctccggca gaacgtcgcc gccgaaccta aaccaacctg tttcgtggat ggcctgagcg
     1141 aacgcgacat ctccacggat tccgtagtac ttgccccatt cgatgtagta tttcccaaga
     1201 ctaggtgcat taggattgat tctcttgacg tagttatcta gctgttctgc agtaagcact
     1261 gacggaccaa aaatcgaagg atcgttccag agatcgccaa cgttaacctt ctctttcttg
     1321 ggtttgaaag tccatgctaa cacccctgca aaaagcacca cgatcagggt ccaaatcaca
     1381 gttttctgca tgtacctctg ctgagttctt gtctagccca agactcagct ctggtcacct
     1441 cctagcaaag catatttacc gaagggtctc ggctagtaac cgagataatc caagagatcg
     1501 ttgtggatct tgagaatctt gtgagcatac tgctggtctg tagcccagct gcctgcaagc
     1561 aagctcagag taggggcttt tgcccttgtt acgagatggt agcggggatc aacgttagct
     1621 gtccaaaaca caggttctgc ggaatatttt cgcacaggta ccggctcccc tgcgcttttg
     1681 gctgtagcat aggcccacag gtgttggaac tgcccgagga ccccttcttc tggagtgttg
     1741 aaggagtgtc ctctagcgcc tccgcccact gcaccgatgc ctgcatagtt gttctgctct
     1801 gggagaacgt cgccgccgaa cttaaaatag gcggtctcgt gcatcgactg tgcgtaggca
     1861 agatctccgg agataacgag ctcattgccg aagaacaggt agtacttggc aatgtagatc
     1921 tgatgtggat tggctttctt ttggtaccgc acaaaatctt ccagctccca aggcgcaaga
     1981 aaggtccttc ccaaaagcgg cgaagcgagc gctttcctga cctgttccac aggggcggaa
     2041 gtaaggttta cctcaacctc caacttttct ttggagagca ccaccatttc agccgtgaac
     2101 acccgtcccg gtgatttggc gtagaaccag tagatgccct cttcggagaa ctctaagagg
     2161 gtagttttgg tgatctcacc tacagatttt ggcacaggat agtcgagatt tgcggggttt
     2221 ttgtcgtgcc agtagaagac ttcccctttg tcgcgatcga taaccacctt tacagtgatg
     2281 gtatcgggaa cgtcaactga agtgacctcg ttttccttga tggtaatcac ctcttcatag
     2341 acgaatgttc tcctggtgag ttttaggttg tactggccag gggacacctc tatcgccggt
     2401 tttgcaccgg ctctggtaaa gccgtagaag gcgtcatcta ggaagattgc tccatcaggt
     2461 aggtcatagt ctatacttag atagcccttt ttagttagag gttcaagatc tatattgacg
     2521 atagctgtct ggcctctaga gacagaaaca gttctgctta gagttttata cccattggca
     2581 aaggctttta cacgataggt gcccggatct agagagtgtg tggtggcacc tctagcgact
     2641 tcctggccat ttgctgtttc caagagaacg tccagatctt cgtggtcggt tttgatgatc
     2701 aaagtgcctg gtttcttcac aaaatcacac ccgctgaaaa ctagaacccc cagcaggatt
     2761 aacgcaatag tcagcagtct gtttgccata ggtcagctcc ttgaaagaaa gcttagctta
     2821 aggtttctca tacaaagact ctacatggta caattggaag tggatattgc ttggtgtaac
     2881 atctaaaacc acaaaaccct gtcccaagtg gatttctgga acgccgaagg acgtaggtgg
     2941 gcaggagaga tgcaggatgt cggtgcagtt tacgatcagc ttgtgtatgt gtccggaaag
     3001 caccgccaca acgcctttcc ctgccaatag gtccaggatc tctttgcgga cttcaggttc
     3061 tgcgttccag tagtttcctc caggctcatc tacgcagttc aggaagaggg gattgtgtat
     3121 caaaatgatt ttcggtccct cgacttccaa ctgcttcttg aggaaggaca tctgttcttt
     3181 ttcttctggc agcccgcttt gaaaaagctg ggagttgagc ataataaact gatatccgtg
     3241 gtggttttct gcatagaagt ccggtccgaa gttttctctg tagtaggcga ttctttcgga
     3301 tgtgatcgta ccttcaactc cttcaaacac cttgttgccg atgtcgtggt tgccaggcac
     3361 aaacagcacg ggcttgttga tatccttgat gcttgcaaaa aagagctcag cttctttttt
     3421 ggaacctgag ttggtgatgt cgccaccgac taagtaaatg tcgggatcgg tggacttggc
     3481 tttttctagt gccatttgcc actgtttgta gttgaagtcg tcagcctcca caaagtgagt
     3541 atctgagaga aaagcaattc taagcggttt tccaacttga ctcattttat ctgctccttt
     3601 ctggtagata agaccgatga tctggtttca tcgttagttc caggtctatt acaaccgcag
     3661 gcactgccgg cgggcaaaat cgcgtctgta ttttcttact cttagagagt tagcctttta
     3721 ttggtattgt ccttctgagg gcaagtctaa agctgagact ccacacagaa tgatcgcttt
     3781 aacctagaag gagtgccgga aagtaaaaaa ccccgctagc caactggatg agaccagggc
     3841 gagcgaggtg agctttattc gggaaacgca agaatcaaca gaatcaagat gagaaaaagc
     3901 gtaaaggccg tctgggcaag gtttttgact tgttggatgt tcatagaatc cctccgcaat
     3961 actttacgca tgaagtggaa gtattgcaaa tgaccgcagg actatttgaa aactcacgct
     4021 ggctggtcgg agatccctat ctactgccat tttgagcaaa cggcgttgct attttcctgc
     4081 ttccggatcg tcttctagga tagcagcctt cttgatgtcg aggcctttgt ctttcagtga
     4141 gtcaatgacc tcccaaagct tagacttgtt ttgctcttct tcaaagaggc tcaaagaggt
     4201 ttccgcagca aggttgctta aacttactcc taggagccgg aacggtcctt tgtagggttt
     4261 ggcaagcttt ttcacttctt ggaaaatgat atctgtgtct gctgtaggct cggggagggt
     4321 ggagctcttc tcgaaggtgg taaagtcggg aagacgcatt tttacagtta ccgtgcggca
     4381 cttcaggttt tgccgtctca gacgccgtgc agctttatct gtaaggtaca aaagctcctt
     4441 gtgcacctgg gacacggaaa tgtcctcagg aaaggtttct tcgttgccga tggatttggc
     4501 ttcgtgagga ggctccacct ggcgattgtc tattccgagg ctgagcttgt ggagctgaaa
     4561 gccctgctta cccagagccc gtaccagcgt gtctactgga gtgttcctta aggtaggaac
     4621 gtctgtaata cccatagatt cgagtttttt gtacgttgaa gggccaacgc cccacatctt
     4681 tcttaaagga atggtgtcta ggaaggtgat cggatcgtct acgatggtca gcccatcggg
     4741 tttttctttg tctgaggcaa gctttgccag gaacttgttg tgggcgatgc cgacgctggc
     4801 ggtaagatca agcctttctt tgattcttcc ctttatctgg gaacccatgt caaaaagacc
     4861 tttgtagaag tgttcgcacc ctgtcatgtc caggaaagct tcgtcgattg acagaggttc
     4921 tacaagaggc gtaaactcat caaagatctg catgagttct ctggaaactg cggcgtattt
     4981 ttccatgttg cctcgaagga aaatgccgtg aggacagagt cgtttggcct gggcgattgg
     5041 ctgtgccgaa tggactccga acttgcgggc ttcataagag caggtcgaaa cgacccctcg
     5101 cggagagttg gggtcgccac cgatgatcac gggttttccg cgaagttctg gattgtcgcg
     5161 gatttcaata gctgcaaaga acgcatccat atccacatgg acaatgatcc tgttcataga
     5221 aataccccca aaggagaaaa gaggtctaaa cgatgctact ggtatttctc aaatcaaatc
     5281 gagaggcaaa agtcttgaac ggctatccca acatctacag agatgagatc aggaaagtcg
     5341 aaggggtcga agaagacgga gatatctgca acgtcttcag cgaagacttt aagtttttag
     5401 gcaagggctt ctactccaaa tccaatgtga gcgtcaggat gcttaccctt gcagacgaag
     5461 agatcgacga ggatttcttc aggaacaggg tagaaaaggc tgctcaaaac cgcaggggtt
     5521 ttggcgactc ctaccgtctg attcacggag aagcagatct tctcccaggt gtgatcgccg
     5581 acaaatatcg ggattttgtt gttctccaga tacgcaatgt cggcatggag cgctacaaac
     5641 cggttttggt ttcggcactt gcccaggttg tcagtcctaa aggcatttat gaaagaagtg
     5701 attttttaag ctcctcttca gatattctca ctcgcaatgt gggactcctc tatggtgaag
     5761 agccgccaga tgaactgaac atcactgagc acgatctgat ctacaaagtc gacattaaaa
     5821 ccggacagaa gacggggttt ttcttcgatc agaaggactc ccgcttattt gttcgcagca
     5881 ttaccccaaa tggcggtttg gctttggatg ccttttccta caccggcggg tttgccctca
     5941 atatggcaaa agcgggagcg agccaggtga tagcccttga taaagacgaa gatgcagtta
     6001 ggctcggcag aatcaacgct gcagccaacg gcttggataa tgttgagttt atcaatgtcc
     6061 gttatgaaga ttacatgcag tcctatacag gcgaaccttt cgacatcatt gtcctcgatc
     6121 cgccttcgat tatcaagaag aaggaagaaa gaaagcaggg agtcgagatc tttcggaaca
     6181 tcgtgagttt gtctgttccg cacctgaaag aagaaggagt tttgggtttg tgcagctgcg
     6241 catatcaaat cgatctggat ctcttgattg aagcggtgcg ccggagttac agtggctccg
     6301 gaaaaaccct gcaagtactg ggggtaacct atcaatcagg agaccacccg tgggtcttgt
     6361 cttatccaga aagcctctat ctcaagtgtc tctgggtcaa ggtgttctaa agcttctgct
     6421 gcagtccgta acaggctgca gcagtttgat tttaaagcag tagagcgact ggagaaaaac
     6481 actaaaaatt ttattgaatt acacgattcg cgagaaggga ttgttaacaa ttcgatggat
     6541 agtggaaaca aatgattttt tcacttccat agatgggagg gagcgaaaat taaaccgaat
     6601 aagtctttta ttttactcat cttcgctgca gtgtttctgt ctggatgcat cagcttgttt
     6661 ggccataggg tggacgtgca ggttacgatc ccgtttttgc aggattaccc cattgatgta
     6721 gtgttgatgg ctttgaagcc agacggaact actgtagtgg agccaatggt gaaaaaggga
     6781 actgagtttc aagcagtgat gacaatcgat cccggtattt ggtcgttgtc ggtggacctt
     6841 acttatcaag ggtacattct caactctatt gttctcgctc cagaagttct cgtcgaaaaa
     6901 gacaccaaaa aactgaggtt tgaccttccc gatgttgtcg ctacagatga gcttttcggt
     6961 gaaatctccg ttcccgattt agtaaagagc ggcgaaaagg taactattaa aacaacagga
     7021 acc
//
LOCUS       DAOSDA010000025         7005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_6929_length_7005_cov_3.797842, whole genome shotgun sequence.
ACCESSION   DAOSDA010000025 DAOSDA010000000
VERSION     DAOSDA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6929_length_7005_cov_3.797842"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..780
                     /gene="jag"
                     /locus_tag="PLW82_01115"
     CDS             154..780
                     /gene="jag"
                     /locus_tag="PLW82_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOJ45908.1"
                     /translation="MIKVEQTAKTVDEAIEIVLSQYNVTRDDIKVTVVDEGKKGFLGI
                     GTKEAKIIVEIKEESEKKQERALEFVKSVVEKMGLNAEIDIKEEDGTLFVNIESPDVG
                     VVIGRRGETLDSLQYLTGLVVNKDSAQWTRVILDASGYRKRREESLVDLAKKKAQRVK
                     HTKRKLSLEPMNPQERRIIHTALQDDPQVTTFSEGEEPYRRVVIALKK"
     gene            942..2300
                     /gene="mnmE"
                     /locus_tag="PLW82_01120"
     CDS             942..2300
                     /gene="mnmE"
                     /locus_tag="PLW82_01120"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOJ45909.1"
                     /translation="MNKDTIAAIATPLGEGGIGIVRLSGNEAINIAQRISSLSLRELP
                     PRRMTLGFVVLEDTRLDRVLWVVMPGPHSFTGEDVVEIHCHGGLKVTELVLEAVIKSG
                     ARLAEPGEFSKRAFLNGKMDLAQAEAIVDVIRAKTATSLRVAFRVLEGHLSEKIEGIR
                     EILLSTLSTIEASIDYPEDVEEPDRDQLKDTLGSITEDLNELIRSYRKGKSLREGYLV
                     TIVGRPNVGKSSLLNHLAKEEKAIVTDIPGTTRDVVEVEINVGGVPVVFADTAGLRET
                     EDKVESIGVGKSKAYLSKADLVLWVLDSSEELTVEDRKIAEMLDGKDVIVILNKQDKP
                     SKISASEIERLVENTTILPLSLTEKTGLDSFMAELENRVSLGGAEAMGEVLITRVRHK
                     NALDNALDAIKHAIGFVPTSPLDLIAIDLRIAYSALGEITGHTVKEDVIDRIFSEFCI
                     GK"
     gene            2285..4177
                     /gene="mnmG"
                     /locus_tag="PLW82_01125"
     CDS             2285..4177
                     /gene="mnmG"
                     /locus_tag="PLW82_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOJ45910.1"
                     /translation="MYRKVGESLYDVIVVGTGHAGCEAALATARLGLKTLALTINKEN
                     IAAMDCNPSLGGPGKAHIVREIDALGGEMGKNIDATYIQLRMLNTKKGPAVQALRAQA
                     DKVKYSARMRSVMEHQENLTVKQGEVTALIFEEGRVAGVVTRTNREYRSKVVILTTGT
                     YLGGRVFIGQEINYESGPSGLRPATEFSKFLRDVMGLKLIRYKTGTSPRVDGRTIDYS
                     KLEEQPVDDVPFSFSYDSTEVRKDFVPCYITHTTVETHRIIEENLTKAAMYSGAITGV
                     GPRYCPSIEAKLVKFPGRTGHQVFIEPEGRYTTEKYLAGASTSLPESIQLEFLRTIPG
                     LENVEIVRPGYAIEYDAIDPTELDLTLELIRFPGIYCAGQINGSSGYEEAAAQGLIAG
                     INAYLKLEGKDPFILDRSEAYIGVLIDDLVTKGTDEPYRMMTSRAEYRLLLRQGNADL
                     RLTPRAREIGLISDERWEKFLSRKNAIEAEVQRLEETKVYPNAETNRILEELGSAALK
                     DVATLADVLRRPEMDYETLKALDPDREPLPSDVIYEVETEVKYKGYISRALSEVAEFK
                     KIESVLLDADLDYSKVKGLSSEAIEKLSAIRPRSVGQASRISGVSPADITALLIYLKG
                     RSSHVE"
     gene            4167..4889
                     /gene="rsmG"
                     /locus_tag="PLW82_01130"
     CDS             4167..4889
                     /gene="rsmG"
                     /locus_tag="PLW82_01130"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOJ45911.1"
                     /translation="MWNSQEKEAFLKTLEGSVPYLTEEKKGRLLTYAAYVVEQNEMIN
                     LTAITKPEEFAIKHIIDSLMVLEHVDLAGKTVIDVGTGAGMPGLIWACVCDDTEFLLL
                     DSLNKRIAFLERAILLLGLKNVRTVHARAEDAGRDKNLRGKFQVATARAVASLSVLLE
                     YCLPFVEVGGAFAALKGPDVDEEVRGSANALKVLGGEISEIASYDLPQGMGSRSLIMV
                     KKVAQTPKTYPRKAGTPNKTPL"
     gene            5511..6242
                     /locus_tag="PLW82_01135"
                     /pseudo
     CDS             5511..6242
                     /locus_tag="PLW82_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256505.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
     gene            6220..>7005
                     /locus_tag="PLW82_01140"
     CDS             6220..>7005
                     /locus_tag="PLW82_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOJ45912.1"
                     /translation="MSRQALGKGLGALLPPKAQDDVKTVEIAKVDPNPYQPRKVFDEA
                     SLQELADSIKEHGVLQPILVRRVEDRYQIVAGERRWRAAGLAGLTEIPAVVKEFDDRL
                     MSQVALVENLQREDLNPLEEAEAYQHLIDEFGMTQETIAESVGKSRTAVANTLRLLRL
                     TEFVKESVSRETISEGHARALLGLNDEKLQDEVCEKVIAEGWSVRATEDYVRRLNEGV
                     TEKTKATKGTKTNIFLQEISEKIGERLGTKVQIKPGKKVNKIEI"
BASE COUNT         2001 a         1464 c         1894 g         1646 t
ORIGIN      
        1 tttccctgcc atcattacgt ttatgggaat caacttccca gcaggatttt cgctttatat
       61 cctcatggtc tacattattt cttacattga agtgattgtg ataaactcat acttaaacaa
      121 gaagaaggcc agcgtaaagg ggggcgcgaa aaagtgatta aggttgagca aacagcaaag
      181 actgttgatg aagcaataga gatagtactg agtcagtaca acgtgacaag agacgacatt
      241 aaggtcacag tagtggacga aggcaaaaaa ggctttttgg gaatcggaac gaaggaagcc
      301 aagatcattg tggagatcaa agaggagtcc gaaaagaagc aagaaagagc cttggagttt
      361 gtaaaaagcg ttgtagagaa gatgggactc aacgccgaga tagacattaa agaagaagac
      421 ggaacgctct ttgtcaacat tgaaagcccc gatgtaggag tagttatcgg tcgccgcggc
      481 gaaaccttag attccctaca gtatctcaca ggacttgtgg ttaacaagga ttcggcccag
      541 tggaccagag tcatcctgga tgcctccgga tacagaaagc gcagagaaga gtctttggtt
      601 gatctcgcca agaagaaagc ccagcgcgtc aagcacacca agagaaagct ttcgcttgag
      661 ccgatgaatc ctcaggaaag aagaatcatc cacacagctc ttcaggatga tccacaggtt
      721 acaacattca gcgaaggcga agaaccgtat cgccgcgttg taattgcctt gaaaaaatag
      781 gaaggtgcgt gcccttttgg cgcgcaccct ctttttaatt atggagcttt tatttaatat
      841 ttaatttggt atatgtacta ttatagaagt aggcaaagaa ttgggttaaa gatccaattc
      901 tcttttcacg gtatagcaag cttctttacg gaggttggaa gatgaacaag gatacaatag
      961 ctgcaattgc tactcccctt ggagaaggcg gcataggaat agtgaggctg agtggcaacg
     1021 aagctatcaa tatagcccag cggatttcga gtttgagtct tcgagagctg cctccccgca
     1081 ggatgacttt ggggtttgtg gttttggagg acactcgctt ggaccgggtg ctgtgggttg
     1141 ttatgccggg acctcacagc tttactggtg aagatgtggt ggagattcac tgtcacggcg
     1201 gattgaaagt cacggagctc gtgctcgaag cggtgatcaa gtcgggagcc cgtttggcag
     1261 aaccaggtga gtttagtaaa agagccttct taaacggcaa aatggacctc gctcaagcgg
     1321 aagccattgt tgatgtgatt agggcaaaga ccgctacttc actccgagta gcttttcgcg
     1381 ttcttgaagg ccatctttcg gagaagatag aagggattcg ggaaattctc ctctctactc
     1441 tctcgacaat cgaagcgagc attgactatc cggaagatgt agaagagcct gacagagatc
     1501 agcttaaaga tactctaggc agcatcactg aagacctgaa cgagcttatt agatcatata
     1561 ggaagggaaa atctctccgc gaaggttatc tggtgaccat cgttggcaga cctaacgtgg
     1621 ggaaatctag ccttctgaat catttggcaa aagaagagaa ggccatcgtc acagacatac
     1681 ccggaactac aagagatgta gttgaagttg agattaacgt cggcggggtt ccggtggtct
     1741 ttgccgatac agcaggtctc agagagaccg aagacaaggt tgaatctatc ggtgtaggaa
     1801 agtccaaagc ctacctaagt aaagcagacc tcgttctctg ggttttggac agcagtgaag
     1861 aactcactgt tgaagataga aagattgcag agatgcttga tggcaaagat gttatcgtta
     1921 tcctgaacaa acaggataaa ccatcgaaaa tctctgcttc cgaaatagaa agattagtgg
     1981 agaacaccac aattctcccg ctgtcactaa ctgaaaagac cggactcgat tctttcatgg
     2041 ctgaactcga gaaccgagtt agccttggag gagctgaagc tatgggagag gttctcataa
     2101 ctagagtccg gcacaagaac gctttggaca acgctctaga tgctatcaag catgctatag
     2161 ggtttgtacc cacctctcct cttgacctga ttgctatcga tctgaggata gcgtatagtg
     2221 ctttgggaga gatcacaggg cacacagtta aagaggatgt tattgataga atatttagtg
     2281 aattttgtat aggaaagtag gtgagagctt gtacgacgta atagttgtag gaacagggca
     2341 tgcagggtgt gaagctgcac tggccactgc cagactgggt ttgaagaccc ttgcccttac
     2401 cataaataag gaaaacattg cagcgatgga ctgcaatcca tcgctcggcg gaccagggaa
     2461 agcccatatt gtaagggaaa tcgatgctct cggtggagag atgggaaaaa acattgacgc
     2521 aacctatatt cagctcagaa tgctgaacac caagaagggt ccagcggttc aagctctcag
     2581 ggctcaagcg gataaagtca agtactctgc cagaatgcgc tcggttatgg agcatcagga
     2641 gaacctcact gtgaagcagg gagaggttac cgctttgatc tttgaagaag gcagagtagc
     2701 tggagttgtg acaagaacca accgagagta tcgttctaaa gtagtgattc tcacaacggg
     2761 aacctacctt ggtggtaggg tctttattgg ccaggagatt aactacgagt cgggacctag
     2821 tggtcttcgt ccagccacag agtttagcaa gttccttagg gatgttatgg gcttgaagct
     2881 tattaggtac aagaccggta cgtctccgag agtcgacggg aggacaattg actacagcaa
     2941 gctggaagag cagcctgtgg atgatgtacc tttctctttc tcatacgaca gtacagaagt
     3001 tcgcaaagac ttcgttccct gctacatcac ccacacaaca gttgaaaccc accgtattat
     3061 tgaagaaaac cttaccaaag ctgctatgta cagcggggcg attaccgggg tcgggccgag
     3121 atactgccct tcgattgaag ctaaactagt taagtttccc ggtagaacgg gacatcaggt
     3181 tttcattgaa ccggaaggcc gttatactac ggaaaagtat ctagcgggag cttccacatc
     3241 cctacctgaa agcatacagc tggagtttct ccgcaccatt cccgggctcg agaacgtgga
     3301 aatcgtccgt cctggatacg caatagaata cgatgccatt gatcctacgg agcttgatct
     3361 cactctcgag ctaatccgtt tccctggaat ctactgcgcc ggacagatca acggcagttc
     3421 gggatatgaa gaagccgcag cgcagggtct aatagccgga atcaacgctt acttgaagct
     3481 cgaaggcaaa gatccgttta ttctcgatcg ctcagaggct tatattgggg ttctcatcga
     3541 cgatctggta actaagggaa cagatgaacc ctaccgtatg atgacaagta gagccgaata
     3601 tagactgctc ctccgtcagg ggaatgccga cttgagactt acaccgaggg cgcgggagat
     3661 cgggctgatc tccgacgaga gatgggagaa gttcttaagc aggaagaatg ctatagaagc
     3721 ggaagttcag cgccttgaag aaacgaaggt ctacccgaac gccgagacaa acaggatctt
     3781 ggaagagctc ggcagtgctg ccctgaagga tgtggcgact cttgctgatg ttctccggag
     3841 acctgagatg gactacgaaa cgctgaaggc attggatcca gatagagaac ccctaccttc
     3901 cgatgtgatt tacgaggtag agacggaagt taagtataaa ggttacatct ctcgagctct
     3961 aagtgaagtt gctgagttca agaagatcga gtcggttctt cttgatgcgg atttggatta
     4021 cagtaaagtt aaagggctct cttcggaggc aatagaaaag ctttctgcta ttagacctcg
     4081 atctgtggga caggcttcaa gaatcagcgg tgtttcacca gcagatataa cggcgcttct
     4141 aatctatttg aaaggaaggt cgtcccatgt ggaatagcca ggagaaggaa gccttcctga
     4201 agacgctgga aggttcggtt ccctatctga cggaagagaa gaaggggaga ctccttacct
     4261 atgcagctta cgtagtagag caaaatgaaa tgatcaacct tacggccatc acaaagcccg
     4321 aggagtttgc catcaaacac attattgata gtttgatggt gttagagcat gtggacctcg
     4381 caggcaaaac tgtgatcgat gtagggacag gcgccgggat gcccgggttg atttgggcgt
     4441 gcgtgtgcga tgataccgag tttttgcttc ttgattcgtt gaataaacga atcgcttttc
     4501 tagaaagagc cattttactt ctagggctta agaacgtcag aaccgttcac gcgcgggcag
     4561 aggatgccgg tagagacaaa aacctgagag gtaagttcca ggttgccaca gcccgtgcgg
     4621 tagcgagtct ttcagttcta cttgaatact gcctgccttt tgttgaggtc ggcggagcat
     4681 ttgcagctct aaaagggccg gatgttgatg aagaagtcag aggtagcgcc aatgcgttga
     4741 aggtcttggg cggtgagatt tccgagattg ctagttacga cctcccgcag ggtatgggaa
     4801 gtcgtagctt aattatggta aagaaagttg ctcagactcc aaagacctat ccccgaaagg
     4861 ctggaactcc caacaagact cccctttaag tgggaggcgg caggaagcaa ctttgcatta
     4921 gagaactata cttaatgaag acctaaaaga aaggaggcgg taggatggga aaagttatag
     4981 cagtcgtcaa ccagaaaggc ggcgtaggcg tgcgccgggc aaagtttgca taagcaatgt
     5041 ggacatgcca cttatagttt ttgctataaa ggctgactag acatccataa tgcgaccaat
     5101 tgcattagcg atgaagctct aggaacaagg caaactcggt ttgccgagcc tgagtcagac
     5161 tatgactacg tacaggaagc tgcttaaaac tcagtgaacg cctaaaactt gttgtcttgt
     5221 gcgacgggga cggcctactc cctatcgtat agcccctgtt tacataaggg aggacaacct
     5281 gggtatagca gcagtctaag gtctgatgta gtgtttatgc aaaaacccgg gaactgttat
     5341 tatccataca aagctatggt cggctggata ataagagggc ggagcactcg tagtagtctg
     5401 aggtagggaa agcctactac atggcgaagg ggtgcagggg tggaaaacat cccggagagc
     5461 cgtgtgaagc gaaagtttca cgcacggttc ggggagaggg gtaagcccta ctccacaaaa
     5521 gtacaacagc aattaatctg gcggctgctc ttagggaact ggataaacga gttttaatca
     5581 ttgataatga tccacagggc aatacaacga caggatttgg tatagacaag gatggacttt
     5641 ataagtgcac atacaacgtc ctagtggaag gcacaccagc aaaagatgtg gtggttgata
     5701 ttaatgggat ttccttgatt cccgctacca ttaacttagc tggagcagag atagaaatgg
     5761 tttcgttttt gtcgcgggag tttcgcttga aaaatgccat tagggacctt gtgccgcact
     5821 atgattatgt tctcatcgat tgtccccctt ctttaggact gctgactgtc aatgcgctgg
     5881 ctgcagctga acgaattatc ataccgatcc agtgcgagta ctacgcgttg gaaggtttaa
     5941 ctcaattgct cgagacggtc gagctggtga agtctaatat caatcctacg ttggcaatag
     6001 aaggcgtttt gcttaccatg tatgatccta ggaccaacct ggcgattcag gttgctgatg
     6061 aagttaagaa atacttcccc gggctcgtct ttgaaacgat cattccgcgt aatgtaagga
     6121 tcagcgaagc tccaagcttt gggctttcgg ttttggagta cgcttcagac tccaagggcg
     6181 cgattgccta cagagaactc gctcaggagg ttttgcagca tgagcagaca ggctctaggt
     6241 aaaggtctag gcgcgctttt gccgcctaaa gctcaggacg atgtcaagac ggtagagata
     6301 gctaaagttg atcctaaccc ctatcagcct aggaaagtct tcgacgaggc atctctccag
     6361 gaacttgccg attccatcaa ggagcacggt gttctccaac ccattctggt aaggcgagtg
     6421 gaagatcgct accaaatcgt cgctggcgag agaaggtgga gagctgcggg cttggccggt
     6481 ttgacagaga ttcctgcggt agttaaagag ttcgacgata ggctaatgag tcaagtcgcg
     6541 cttgtagaga acctgcagag agaggactta aatccgttgg aagaagctga agcatatcag
     6601 catcttattg atgagtttgg tatgacccag gaaactatcg cagagtccgt gggtaaaagc
     6661 cgtacggcag ttgcaaacac gctgcgtctt ttgcgattaa cagagttcgt gaaggaaagt
     6721 gtttcacgtg aaacaatttc tgaaggccat gcgagagctc tcctcggact gaatgatgaa
     6781 aagcttcaag acgaagtttg cgaaaaggtc atcgcagaag gctggagcgt aagggcaacg
     6841 gaagattatg tccgcagact gaacgagggt gtcaccgaga agaccaaggc cacaaagggc
     6901 acgaagacga acatctttct gcaagaaatc tcggaaaaga tcggtgagcg tttaggaact
     6961 aaggtacaga tcaaacccgg gaaaaaggta aacaaaatag aaatc
//
LOCUS       DAOSDA010000026         6954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_6990_length_6954_cov_3.578924, whole genome shotgun sequence.
ACCESSION   DAOSDA010000026 DAOSDA010000000
VERSION     DAOSDA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6990_length_6954_cov_3.578924"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PLW82_01145"
     CDS             complement(<1..853)
                     /locus_tag="PLW82_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ45913.1"
                     /translation="MLLELNVEDFALIDRLSLQFGPGLNIITGETGAGKSILLDAVSL
                     LLGARSTKEMIRTGSEKTRIEGVFDIGAVDGAKEICSEAGYDVSDDALIIVREVNLDG
                     RNRCYVNGRACTVSFLAELGKKLVDLHGQHAHQSLLYPENHLRLLDAFGGDELLRTKA
                     EYLKSYDAWQKLKRRLAQIINLKEERQKKKELLEYQLAELEKADLKAGEREILEAERS
                     RLKSADALYNLSYQSSQLLSNSGYDIDAFEILNPLRVVKENLEKLARIDEGTRAYLDE
                     AADCLYRL"
     gene            complement(867..1322)
                     /gene="argR"
                     /locus_tag="PLW82_01150"
     CDS             complement(867..1322)
                     /gene="argR"
                     /locus_tag="PLW82_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOJ45914.1"
                     /translation="MKAKRQALILELIAKEALDTQEQLVDRLKHMGIEVTQATVSRDV
                     KELRLIKVAVGDGRYRYAPPPDAFDKPEDIKRARRIFQEAVLSIDSSLNLVVIKTLTG
                     TAQGVAAVIDGMGISGVLATVAGDDSILVVVKPEEAVGTVIQELEALRS"
     gene            complement(1336..2214)
                     /locus_tag="PLW82_01155"
     CDS             complement(1336..2214)
                     /locus_tag="PLW82_01155"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOJ45915.1"
                     /translation="MKTQRSIAIIPHTGKPLALSVAGRTAELLEKHGAKVLVPREYAK
                     GDEMLKYASETALDEADLIVVVGGDGSILKASQEARKRRIPLLGINTGHIGFLTELEV
                     DEVEKYIRDIAEGNYEIEERILIKAQIHGKEEQSEVFYCLNDAVLSRGAAARLIYFDV
                     YVDDSFAGQYPADGIVVATPTGSTAYSLSAGGPLVDPEVSCVLLTPVCPYMLSARPVV
                     VGVQHKIKIRVSESDEDVLLSVDGILKGTLTEGEELIVEADEERLMFAKLNVRTFYDV
                     LRGRFQSCRNMNTPEN"
     gene            complement(2254..3039)
                     /locus_tag="PLW82_01160"
     CDS             complement(2254..3039)
                     /locus_tag="PLW82_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOJ45916.1"
                     /translation="MRLDQYLVAEGYFPSRSKAKKAIEDGKVLVDGKLAKASFLLSGS
                     ESIEVLPEEVQFVSRGGFKLLHALEVFGISVTGRRALDIGASTGGFTDCLLQRGAAQV
                     VAVDVGRDQLDESLKHDPRVISLEKTNIRELQPEAVGRFDIITIDVSFISLTKFADRL
                     PLFLRETGDVVALIKPQFEAGPENVGKGGLVKDPKVHLEVLENVIRAFADAGLYAWGA
                     AYSPIAGGSGNIEFLGWFRLQENTDPGYDLKSIVAEANMQVIR"
     gene            complement(3036..4925)
                     /gene="dxs"
                     /locus_tag="PLW82_01165"
     CDS             complement(3036..4925)
                     /gene="dxs"
                     /locus_tag="PLW82_01165"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOJ45917.1"
                     /translation="MSRLLDRLSSPSDLRGYSYHELNILAKEIRDEIIHTVSLNGGHL
                     APNLGVVELTIAIHRVFDSPRDKIIWDVGHQCYTHKLLTGRRDAFFTLRQLDGISGFP
                     KPEESIHDAFATGHSSTSISAALGMATARDLNGEDYKVVAVIGDGSITGGMAYDALNH
                     AGHLKKNLTVILNDNEMSIAKNVGALSRYLSDLRAAPGYSKLKSNVQSVLDKVPVVGK
                     PLARAIEKAKNTFKYMLVPGVFFEELGFTYLGPIDGHNIPLMCKVFSEASAIKGPVMV
                     HVITQKGRGYKPAEDVPSKYHGVGPFDVNTGKIKSGSAASFTSCFAEALLSEAEENPH
                     IVAITAAMTEGTGLLEFSQRYPERFFDVAIAEQHAVTFAAGLAKAGKKPVVAIYSTFL
                     QRAYDQIVHDVCLPNLPVVFAVDRAGLVGPDGPTHHGIFDLSFLRSIPNITVFSPKDG
                     RELADMLHTALRLDGPVAIRYPKHETSAISSGPRTFIVPGKAEVLSLGRHANIAAEGA
                     MVEVAVRVREKLAAEGFDVGVVNIRSIKPLDGELLCSLADKPLVTLENNSLEGGLGAA
                     VLEALNRAGSSAEVKCIGLPGKFIPSGGVQELHQRLGLDDESIAMNIRQFLGLLRVVP
                     EVASK"
     gene            complement(5182..5736)
                     /locus_tag="PLW82_01170"
     CDS             complement(5182..5736)
                     /locus_tag="PLW82_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45918.1"
                     /translation="MIVTFIAVLVVSFLLNAGADSLIASAGLVPSLLGLIVIILIGVV
                     FDMVGTAVAAAEEAPLHAMAGDKVPGAKQAIWLTRNADSVASLCNDVIGDICGTLAGA
                     AAATIAYRLSTTSYFLRVFMAAMAAALTIGLKRAEKSFALGKATDIIYFVGKMIFYFE
                     KATGMKITSTKKNNQKRKNRNGQH"
     gene            complement(6504..6947)
                     /locus_tag="PLW82_01175"
     CDS             complement(6504..6947)
                     /locus_tag="PLW82_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOJ45919.1"
                     /translation="MNIITVVWDNLAWRSAFIAWAIAQFLKGFTSIRPRGFSWKHFLG
                     TGGMPSSHSAFVSGLTTAVGILEGFGSTLFAACFVFSMIVMYDAAGLRRAAGKQAAIL
                     NKLIDELIHTGELKQARLKELLGHTPYEVFAGASLGILVAVLVCR"
BASE COUNT         1658 a         1898 c         1607 g         1791 t
ORIGIN      
        1 caagccggta taggcagtct gcagcctcat cgagataggc tcgggttccc tcgtcgattc
       61 tggcgagttt ttccaggttt tctttcacca ctcgcagtgg attgagtatt tcaaaggcat
      121 caatatcata tccgctgttg gaaagcaact gactggactg atagctcaag ttgtagaggg
      181 catcggcgct tttcaatctg cttctttccg cttcgaggat ttctctctct ccggctttca
      241 gatcggcctt ctccaactct gccagttggt actccaggag ttccttcttt ttttgccgtt
      301 cttcctttag gttgataatc tgtgccagac gccttttcag cttctgccag gcatcgtacg
      361 atttaaggta ctcggccttg gtcctgagaa gttcatctcc gccgaaggca tccagaagcc
      421 tcaggtgatt ttcgggatag agcagcgatt ggtgagcatg ctgaccgtgg aggtccacaa
      481 gctttttacc gagttctgct aaaaagctaa ctgtacatgc cctgccgtta acatagcagc
      541 ggtttctccc atcgaggttg acctcgcgaa caataatgag ggcatcgtca gaaacgtcgt
      601 agcctgcctc gctgcagatc tcttttgctc catctacagc accgatatca aagactccct
      661 cgatcctagt cttctcgctc cccgttctaa tcatctcttt agtagacctt gcaccaagga
      721 gaaggctgac tgcatcgagc aagatggatt ttcccgcacc ggtttcaccg gtgatgatat
      781 ttagccccgg accaaactgc agggagagtc tgtctattag ggcaaagtcc tctacattta
      841 gttccaaaag cacaagtttc acctgcctag cttcttaaag cttcaagctc ttggatcacc
      901 gttcccactg cttcttccgg cttgactaca accagaatcg aatcatcgcc ggcaactgtg
      961 gctaaaactc cggagattcc catgccgtca atgacagcag ccacaccttg tgccgtgcct
     1021 gtcagggttt taatcaccac cagattcagc gatgagtcga tgctgaggac cgcttcttga
     1081 aagatgcgcc gtgctctttt gatatcctct ggtttgtcga aagcatcagg aggcggtgca
     1141 taacgatagc gaccgtctcc aacggcaact ttaatcagcc gcagttcttt gacatcgcgg
     1201 ctcaccgttg cctgagtcac ttcaatcccc atatgcttta gacggtctac aagctgttct
     1261 tgggtatcaa gggcttcttt ggcaatcagc tccagaatca aagcttgtct tttggctttc
     1321 atctgctaac ctcccttagt tctctggggt attcatattc cggcagcttt ggaaccttcc
     1381 ccgcagaacg tcgtagaagg tcctaacatt cagcttggca aacatcaagc gttcttcgtc
     1441 agcctcgaca atcagctcct ccccttctgt cagagtgccc ttcaagatgc cgtcaactga
     1501 aaggagaacg tcttcgtcgc tctcgctgac tcgtatttta atcttatgct gcacgcccac
     1561 aacaactggc cttgccgaga gcatgtaggg acagacaggt gtaagcagaa cgcaggaaac
     1621 ctcgggatcc accagcggcc cgcctgcaga gagagagtag gccgtggagc cggtaggcgt
     1681 tgcaaccaca attccatctg caggatactg cccggcaaag ctgtcgtcta catagacatc
     1741 gaaatagatc aacctagcag cggctcctct ggaaagcacc gcatcgttca ggcagtagaa
     1801 gacctcgctc tgctcctctt tgccgtgaat ctgagctttg atcaggatgc gctcttcaat
     1861 ctcgtagttt ccttctgcaa tatcgcgaat gtacttttct acttcatcaa cttcgagttc
     1921 ggttaagaac ccgatgtgtc cggtgttgat ccccagaagg gggattcttc tttttcttgc
     1981 ttcctgggac gctttgagta tggaaccgtc gccgccgacg accacaatta ggtcagcttc
     2041 atctagagca gtctcgcttg catatttaag catttcgtcg cctttggcat actcccgtgg
     2101 caccagcact ttggcaccat gtttttccag gagctcggcg gtacgtcctg ccacacttaa
     2161 ggctaatggc tttcctgtgt gaggtataat tgcaatgctt ctctgagttt tcaagcgaga
     2221 cacctcctct tttgtattac cattcgccca atattatctt attacctgca tattagcttc
     2281 tgcaacaatg gactttaaat cgtaacctgg gtctgtattt tcttgcaacc taaaccaccc
     2341 taaaaattca atgtttccac tgccgccggc aattggtgag tatgctgccc cccaggcata
     2401 gagccctgca tcagcaaaag ccctgataac gttttccaga acttccagat ggactttggg
     2461 gtccttaacc agaccgcctt ttcccacatt ttcaggaccg gcttcaaact gcggtttgat
     2521 cagcgcaacc acatcgccgg tctctcttaa aaacaaaggc agcctgtcgg caaacttggt
     2581 tagggaaata aaggagacgt cgatagtaat gatatcgaac cttcctaccg cttccggctg
     2641 gagctctctg atgttagttt tctcaaggga gatcacccga ggatcatgct tcaaggattc
     2701 gtcgagttga tccctgccca catcaaccgc aacaacctgg gccgcaccgc gctgcagcag
     2761 gcagtcggtg aatccgcctg ttgacgctcc gatatccagg gcccttcttc ctgtaacgct
     2821 tattccgaag acctccagag cgtggagcag tttaaaccca ccgcggctga caaactggac
     2881 ttcttctggc aaaacttcga tggactcgga accgcttagc aaaaagctgg ctttcgccag
     2941 cttgccatct actaaaactt tcccatcttc tattgctttt ttggcttttg aacggctggg
     3001 aaagtagcct tccgccacta agtattgatc tagtctcatt tggaagccac ctccggcacc
     3061 accctcagaa gaccgagaaa ctgcctgata ttcatggcga tgctctcatc gtcaaggccc
     3121 agacgctggt gcagctcctg tacgcctcct gaaggaataa acttgcctgg aagaccgatg
     3181 cacttcactt ctgcgctaga tccggctctg ttcaaggctt caagaactgc agcacctaag
     3241 cctccctcca agctgttgtt ttccagtgtt acaaggggtt tatctgcgag agagcacaaa
     3301 agctccccat ctagaggctt gatggagcgg atgttgacca cgccaacatc gaagccctct
     3361 gcggccaact tctctctaac tctgacagct acctccacca tagcaccttc ggctgcaatg
     3421 ttggcatggc gccccagact gagaacttct gctttaccgg ggacgatgaa ggtccttggt
     3481 ccagatgaga tagccgaagt ttcgtgcttg ggatacctga tagccaccgg accgtcaagc
     3541 cggagggcag tgtgcagcat atctgcaagc tctcttccgt ctttaggcga gaaaaccgta
     3601 atgttcggta tggacctgag aaaagacagg tcgaaaatac cgtggtgggt tggtccgtcg
     3661 ggacctacca gtcctgccct atctacggca aacaccaccg gaagattggg caagcagaca
     3721 tcgtgaacga tctggtcgta tgccctctgg aggaaggtgg aatagattgc tactacaggc
     3781 ttcttgcccg ctttagccag accagctgca aaggtcactg cgtgctgttc ggcaatagcc
     3841 acgtcaaaaa accgctccgg atacctctgg gagaactcca gaaggccggt tccttcggtc
     3901 atagcggcgg taatagctac gatatgagga ttctcctcag cctccgacaa cagtgcctcg
     3961 gcaaaacagc tggtgaacga agctgcagat ccggacttaa tctttccggt gttcacgtcg
     4021 aaaggcccga ctccatgata cttggaaggt acgtcttctg caggtttata ccccctgccc
     4081 ttttgggtga taacgtgaac cataacggga cctttgatcg ccgacgcttc gctaaagacc
     4141 ttgcacatca gcgggatgtt atgtccgtcg atcgggccga ggtaggtaaa gcccagctcc
     4201 tcgaaaaaga cgccgggaac cagcatgtat ttaaaggtgt ttttggcctt ttcaatggct
     4261 ctggccagag gctttccgac aacgggaacc ttgtctaaaa cgctttgaac gttggacttt
     4321 aatttggaat agcccggtgc agctctaaga tcagacaagt accgggacag cgcaccgacg
     4381 ttcttggcaa tggacatctc gttgtcgttc agaatcaccg taaggttttt tttcaggtgc
     4441 ccggcatggt tcagagcatc gtaggccatt ccgccggtga tagagccatc tccgatcacc
     4501 gctacaactt tatagtcctc tccgtttaga tccctagcag ttgccatacc caaagctgcc
     4561 gagatgctgg tagagctgtg gccagttgca aaagcatcgt gaatggactc ttcaggtttg
     4621 ggaaagcccg agatgccgtc aagctgcctg agggtaaaga aagcgtccct tctccctgta
     4681 agcagtttgt gggtgtagca ctggtgccca acatcccaga tgattttgtc cctgggactg
     4741 tcaaacaccc tgtgaatggc gatggtcagc tctaccacgc ctagattggg agccaaatgc
     4801 ccgccgttta aagacacggt atgaatgatc tcatcgcgga tctctttggc cagtatgttc
     4861 aattcgtgat acgaataacc tcgcaaatcc gatgggctcg aaagcctatc tagaagtcta
     4921 ctcatttcga taccccaatt cctcgcggta ttgatctgtt ctctttttcc ttttgcactt
     4981 ctctcaatag tgtttcagaa tcccgcggat ttttgctttc acattcaggt tgctctttta
     5041 agtgaaagta tatcattaca caggtcccac ctgcaacaaa gcccaaaaaa acacaaaaac
     5101 cgagctgcgg cttcggctct ttgccaataa aagccgcaat agggcacagc tttgcagttg
     5161 tgcccgaaag ctctgcagtt actaatgctg accgttgcgg tttttgcgtt tttggttgtt
     5221 cttcttggta gaggttatct tcatacctgt agccttttcg aagtagaaga tcattttgcc
     5281 tacaaagtag atgatgtctg tggccttgcc cagggcaaag gacttctcgg ctcttttcag
     5341 gccaatggtt agtgctgcag ccatcgctgc cataaagacc cttaagaaat aactggtagt
     5401 ggataaccta taggcgatag tggcagctgc cgcaccggct agtgtgccgc agatgtctcc
     5461 gataacgtcg ttgcacagag atgccacact atcggcattc ctagtgagcc atatggcctg
     5521 ctttgcaccg ggaactttgt ctcctgccat ggcatgcagc ggggcttctt cagcagcagc
     5581 cacagcggtt ccgaccatgt caaaaactac accgatgaga atgatcacga tcaagcccag
     5641 tagggagggc acaagccctg cgctggcaat cagcgaatct gctccggcat tgagaagaaa
     5701 actgacgaca agtactgcga taaaggtaac aatcacgctt cgccaatggt ctgacaaaaa
     5761 ccatcactct ccgagttcat gaaataagga agaccataaa caacttttta ggttggaaca
     5821 aaactaaatg agaaaaaagc tgaagcctgg gcttcagctt cgggttttat gtatccaggg
     5881 aggtgaccac ttcttaggtt aattctatgg cttaaggtct agcaggattt cccaaaagcc
     5941 gcacttagtg tcgccacgta agcggttccc ctttaagggc ttttctgctt tgtcgccaga
     6001 agcagggcta gattaccact cagaccatac tttaatccct aagaagcgta ttacaacagc
     6061 ctaggagttt tcctcaacat cagttgtcta ctatctagcc tcttttgcag gagagatttc
     6121 aaacagcgcc gggccaccaa ctgtggatcc gccagtcagc ggcttgaacc tgttctccct
     6181 cttcccccac tcaaggcaag ctacactgca tgtaacattt gcggcacgtt aaccggctat
     6241 atcacattgc aggttcttcc taagccgaag gatcggccgt agctttcacc ctcgcactta
     6301 ggtctccgcg aagaagggtc aacgcccaca tgcctttgtg gatcgcccct cttcttaacc
     6361 cccagcataa gcccaagact gagcgtccca agccaacacc agggacttca tcgatgtgcc
     6421 ctttaacgga tttttaggcc cgtcttcgat aatagagctg atgactagga tactgtgtca
     6481 cctcaacctc tatatgcatt atatcatctg catactaaaa ccgcaaccaa gatgcccagg
     6541 gaagctccgg caaacacttc ataaggtgta tgtcctagaa gttccttcaa ccttgcttgc
     6601 ttgagttcgc cggtgtgaat aagctcatct attaacttat ttaatatggc agcctgtttt
     6661 cctgctgccc ttctgagtcc ggcggcatcg tacattacga tcatagaaaa cacaaagcac
     6721 gctgcaaaca acgtgctgcc aaagccttca agaataccaa cggctgtagt gagtcccgag
     6781 acaaacgccg aatgagagga aggcattcct cccgtgccta agaagtgctt ccagctgaac
     6841 cctctcggcc tgatggatgt aaaaccttta agaaactgtg ctatggccca ggcaataaac
     6901 gccgagcgcc aagccaagtt atcccatact acagtgataa tgttcaaaca gtcg
//
LOCUS       DAOSDA010000027         6948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_7002_length_6948_cov_3.919484, whole genome shotgun sequence.
ACCESSION   DAOSDA010000027 DAOSDA010000000
VERSION     DAOSDA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7002_length_6948_cov_3.919484"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLW82_01180"
     CDS             <1..222
                     /locus_tag="PLW82_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015592906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ45920.1"
                     /translation="NLLKLFSDINRLGQTVLMVTHSTKAASNASRVLFIKDGEIFHQL
                     HKGASSNEEQYQKIADTLTMIATGGIRHE"
     gene            215..2215
                     /locus_tag="PLW82_01185"
     CDS             215..2215
                     /locus_tag="PLW82_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ45921.1"
                     /translation="MSKFFYARLAINNIRRNAQTYVPYIVTAIGAIMMFVIIGMMTNN
                     TQLLKLRGGTTLRTILFFGTIVVGIFSLIFLFYTNSFLVKRRKKEFGLMNILGMDKRH
                     LARVMFLETLIVAVISITLGILSGVLLSKLMFLVLLKILNFEVQLGFEFDAYFTLFTA
                     MLFAGIFFLSLLNNLRQVQLANPIELVKGGQVGEREPKTNWPAFLLGLITLGSGYYIA
                     LTMENPSTTIPAFFGAVVLVIIGTYCLFTAGSIWFLQLLRKNKKFYYHPQHFPNISGM
                     IYRMKQNAVGLANICILSTMVLVMLSTTLSLYIGMEDVLRTRYRRNFELTASDIDSGA
                     VQRIRDAVDSVLAKRGKAPQDIISYRYLALTAKQEGSSFATRKNDLDMFSTALKYVYF
                     VPVEDYNALTNRSVVLEDNEALICSAREAYDYDSLSILGRTFTIKEKVDSFQGDGSSL
                     VFLYSSYYVVVKDMDTVQEMYHKYTAENELYRPTISYYFGFDVDANRSESLAIHDDLV
                     ASAKQAIAGSGYVVVESAENARDDFYSLYGGLFFLGIFLGVLFIGATVLIIYYKQITE
                     GFEDKHRFEIMQKVGMSRLEVKKTIKDQVLMVFFLPLIAAIIHIAFAFKPITKMLALF
                     NLTNIALFIWCTLGVILVFGLFYAVVYVLTARVYYQIVSAKE"
     gene            2415..2491
                     /locus_tag="PLW82_01190"
     tRNA            2415..2491
                     /locus_tag="PLW82_01190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2449..2451,aa:Arg,seq:cct)
     gene            2715..3092
                     /locus_tag="PLW82_01195"
     CDS             2715..3092
                     /locus_tag="PLW82_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ45922.1"
                     /translation="MDIIISNSSGKPIYEQITSQIKNLVITGELKPGDPLPSIRVLAK
                     ELRISVITTKRAYEDLERDGFIVSVPGKGTFVADQSIEMLRERQLKIVEEHLQTGVDA
                     AKMAGISLDELVEMLGILYGEEE"
     gene            3096..3947
                     /locus_tag="PLW82_01200"
     CDS             3096..3947
                     /locus_tag="PLW82_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ45923.1"
                     /translation="MENVLEVRKLRKKYDSFELQDISLDLPRGSIMGFVGQNGAGKTT
                     AIKLILNLIRADAGEIKIFGEKHNALDRKLKEQIGVVFDECNFPEYLTAKDIILVMKN
                     IYRSFQVERFDKLLDSLKLPKNKPIKEFSRGMKMKLSIAVALSHGAKLLILDEPTSGL
                     DPIVRDEVLDLFLEFIQDEDCAILVSSHIISDLEKVADYITFIHEGKIIFSENKDRLL
                     EEYGVLKCSHHDFEQIDKTKVLGYRENQFSVAALIRKKDVPKGFLVEQASIEEIMLFM
                     TRGERAS"
     gene            3944..4564
                     /locus_tag="PLW82_01205"
     CDS             3944..4564
                     /locus_tag="PLW82_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOJ45924.1"
                     /translation="MKGLLIKDFLILKNQGKLLALALLVYSIIAFSSGNADMVTTMLF
                     VVSVIMPVTAIAYDEKSRWERYALATALNRRDLVLSKYLFGGLCIVCGMLLGILFTLA
                     AEKGSAAVLLPRLRMLVVAACVGMVVQSVNLPIVFHFGVEKGRIAMLLVIAGTAALQF
                     YLGQVGIQVSGSLFSNMPLVLYVVIAAVSFGLSIVVSNLIYSKKEF"
     gene            complement(4789..5241)
                     /locus_tag="PLW82_01210"
     CDS             complement(4789..5241)
                     /locus_tag="PLW82_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOJ45925.1"
                     /translation="MKTAIIYYSKHHGNTKKLLDQIATQSDITLIDASTTLDADLSGY
                     DLIGFASGTYFSKLHQSVIEFAERNLPMEKKVFVISTYGGVKPRIHNLKKIISLRNGQ
                     FVGHYGCKGYDTYGLFKIIGGIAKGRPNAKDVARAVKFFNNLVERYGG"
     gene            complement(5447..6685)
                     /locus_tag="PLW82_01215"
     CDS             complement(5447..6685)
                     /locus_tag="PLW82_01215"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ45926.1"
                     /translation="MRLVSNPEPEAGGYKKDTIVELQAIPADNWKFAHWTGDIDGNSQ
                     VITVKMDSPKNIKAVFSKDTHFIYVIVQGNGKVEETVLAGRGVYEYGSVVELKAVPDD
                     GWLFSHWELDATGTTNPLVVYVDKSRQVRAVFKESSLRVPEQYKTIQAAINAAGPGYE
                     IVVSPGTYNENLDLTGKKNITIRGTNPDDPLTILATVIDGDKKGPCILIDQYADVTIS
                     GLTLTNGSGVPMSGDYAYGGGIYLGQYSKAVISNCAITGNTGSHGAGIYSNNSTLTLT
                     DTLISDNYASFTGAGIWMSNGQGANTISNCTIINNEASYGGGMYISFSSVSITDNDIG
                     GNTAAIGGAAMFATSANITFVDNNVTNNSSTTSDGVVSIAQSVKVKDKDGNEMTTEEE
                     ILAVNDFSDNTPGGVQFTES"
BASE COUNT         1818 a         1518 c         1634 g         1978 t
ORIGIN      
        1 aacctgttga aactgttttc cgatatcaac aggctaggcc aaacagtact gatggtgacc
       61 cacagcacta aggctgcgag caatgcaagc cgcgttctat ttatcaaaga cggagagatc
      121 ttccatcagc tgcataaagg cgcaagttcc aacgaggagc agtatcaaaa aatcgcagat
      181 accttgacga tgattgccac cggaggtatc cgccatgagt aagttcttct atgcgagact
      241 tgcaatcaac aacatccggc gaaacgctca gacttatgtt ccttacatcg ttaccgctat
      301 cggtgccatc atgatgtttg tgattattgg catgatgacc aacaatactc agttgttgaa
      361 gctgagaggc ggcaccacgc tccggacaat cctctttttt ggaacgatag tggtggggat
      421 cttctctctg atctttctgt tctacaccaa cagttttctg gttaagaggc gtaagaagga
      481 atttggtttg atgaacattc ttgggatgga taagcgccat cttgcaaggg tcatgttctt
      541 agaaactcta attgttgccg ttatttctat cactcttggg atactcagcg gcgtcctact
      601 ctcgaagttg atgttcttgg ttctattgaa gatccttaac ttcgaagtgc aactggggtt
      661 tgagtttgat gcttatttta cgttgtttac agcaatgctt ttcgcaggaa tctttttcct
      721 cagcttgctt aacaacctga ggcaggttca gttggcaaac cctattgagc tggtgaaagg
      781 tggccaggtt ggggaaaggg agcccaagac aaactggccg gctttcctct tgggactaat
      841 caccctcggc tcgggctatt acattgctct cactatggaa aacccgagta caaccattcc
      901 ggcctttttc ggtgctgttg ttttggttat catcggaact tactgcttgt ttactgcagg
      961 aagcatctgg ttcctgcagt tgctgcgcaa aaacaaaaag ttctactacc atccgcagca
     1021 cttccccaat atctctggaa tgatctatag gatgaagcag aatgctgtag gactggctaa
     1081 tatctgcatt ttgtccacga tggttctggt catgctttca actacgctgt ctctgtatat
     1141 cggaatggaa gatgttctaa gaaccaggta tcgtcgcaac ttcgagctga ccgcatctga
     1201 tattgacagc ggagcagttc aaagaatccg tgatgctgta gacagtgttc ttgcaaaacg
     1261 cggaaaagcc ccacaggata ttattagcta cagatatctg gccttgactg caaagcaaga
     1321 aggaagctct tttgcaaccc gaaaaaacga tctggatatg ttcagtactg cactgaagta
     1381 tgtgtacttc gttccggtgg aagactacaa tgcactcacc aatcggtctg ttgtactaga
     1441 agacaatgaa gccctgatct gttcggctag agaagcgtac gattacgata gtttaagtat
     1501 cttgggacga acatttacga tcaaagaaaa agtcgactct ttccagggtg atgggtccag
     1561 tctggtgttt ttgtacagca gctactatgt agtggtaaaa gacatggata ccgtccaaga
     1621 gatgtaccac aagtatacgg cggagaacga gctttaccga cctaccatat cttactactt
     1681 tggattcgac gtagatgcaa accgcagcga gagccttgca attcacgacg atcttgttgc
     1741 gagcgctaag caagccatag ctggctcggg ttacgttgtt gttgagtctg cagaaaacgc
     1801 tagagatgac ttctattccc tgtatggagg actgttcttc cttggaatct tccttggggt
     1861 gctgtttatc ggtgctacag tgctgatcat ctactataaa cagatcactg aagggtttga
     1921 agacaaacac cgctttgaga tcatgcagaa ggttggcatg agtcgtttag aggtaaagaa
     1981 aaccatcaaa gaccaagtgc ttatggtgtt tttccttccc ttaattgcag caatcatcca
     2041 catcgcattc gcgttcaagc caatcactaa gatgcttgca ctcttcaact tgacgaatat
     2101 cgctctattc atatggtgta ccttgggagt catcttggtc ttcgggctat tctacgcagt
     2161 ggtttatgtt cttacggcaa gagtgtacta tcagatagta agtgccaaag aatgaatttg
     2221 ggaaggaagc atagcagaaa cacagaaaga gaactagcaa gtgttttgct ggttctcttt
     2281 cgctcaaatc catgttccac agtgatcaaa caccagataa atcttaggtt ctggaacaga
     2341 tcatgaggtg gaatccatta cctacctatt tctttacact gatttgttta ttgtgctata
     2401 atattaagcg aagtggcccc gtagctcagt cggatagagc aacggtttcc taaaccgtgt
     2461 gtcggacgtt cgagtcgtct cggggccgcc atacataatg cctgctcgag acctgtttga
     2521 ggtctctttt ttgttgcggt tttgtcagaa gccatacgtc tgttggctga aatagaacag
     2581 acctgttact cgatggcggg cgtgaacagt attacgttca tttttcacac ggtatggaac
     2641 ttaaaacctc ttgacgaaca gtgtatagtg tatataatga atatatacac tatacctttt
     2701 aagggctgat tgaaatggac attattatca gcaattccag tggtaagcca atctatgagc
     2761 agatcacctc acaaatcaaa aacctggtca tcaccggtga attgaaacca ggtgatccgc
     2821 tcccgtccat acgagtgcta gccaaagagc tccgaataag cgtcatcacc acgaagcggg
     2881 cttatgaaga cctggaacgg gacggtttca tcgtgtctgt tccaggcaag ggcactttcg
     2941 ttgccgatca aagcatagag atgcttcgcg aacggcagct taagatcgtg gaggaacatc
     3001 tccaaacggg tgtagatgcc gcgaaaatgg ccgggatcag tttggacgag ctagtggaga
     3061 tgctgggcat tctttacgga gaggaagagt gacatatgga aaacgttttg gaagtgagaa
     3121 agcttcgtaa aaaatacgac tctttcgagc tgcaggatat cagcctcgac ttgcctaggg
     3181 gaagcatcat ggggtttgtt gggcaaaacg gagctggaaa aactactgca atcaagctga
     3241 tcttgaacct aattcgtgct gatgcgggag agataaagat ttttggagaa aaacacaatg
     3301 ccctggacag aaagttaaaa gagcaaattg gagtagtatt cgatgaatgt aattttcccg
     3361 aatacctaac agctaaggac attattctgg taatgaaaaa catctaccgt tccttccaag
     3421 tcgagcgttt tgacaaactg cttgactccc tcaagcttcc gaagaacaag ccaattaaag
     3481 agttttcccg cggcatgaaa atgaaactgt ccattgctgt agcactgtca cacggggcga
     3541 aactgcttat ccttgacgaa ccaaccagcg gtctcgatcc cattgttcgg gacgaagtat
     3601 tggacctttt tttggagttt atccaagacg aagattgcgc catacttgtt tcatcccata
     3661 ttatcagcga tttggagaaa gtcgccgact acattacttt tatccacgaa ggcaagatca
     3721 tcttcagcga aaacaaagat cggctgctgg aggaatacgg tgtactcaag tgctctcacc
     3781 acgacttcga gcagattgat aaaactaaag tgctcggtta tagagagaac cagttcagcg
     3841 tagcagctct gataagaaaa aaagacgtac ctaaaggttt tttggtcgaa caggctagca
     3901 tcgaagaaat catgcttttt atgacaagag gtgaaagagc gtcgtgaagg gtttgcttat
     3961 caaagacttt ctgatactaa aaaaccaagg aaaacttctg gccttggcgt tgctcgtcta
     4021 ctcaataatc gcgttttctt ccggcaatgc cgatatggtc acaacaatgc tttttgtcgt
     4081 gtctgtgatc atgccggtaa ctgcaattgc ttacgacgaa aaaagccgct gggagaggta
     4141 tgctctggca actgcactaa acaggcgcga tttggtgctt agcaagtact tgttcggcgg
     4201 gttgtgcatt gtctgtggga tgcttttagg catactcttt actctcgctg cagaaaaggg
     4261 gagcgctgca gttttgctac caagactcag gatgcttgtg gttgcggcct gtgtaggaat
     4321 ggttgtacag tctgtaaacc tgccgattgt gtttcatttc ggcgttgaaa aaggcaggat
     4381 tgccatgttg ctggttatag ctggcactgc agcactccag ttttacttgg gccaagtagg
     4441 catacaggta tccggttcac tgttttccaa tatgccacta gttctttacg ttgttatcgc
     4501 tgctgttagc tttggccttt cgatagtggt ttcgaacctc atctatagca aaaaagagtt
     4561 ttagaagagc tggtacattg tcaccagctt tcttatctaa aacgagatag acgggaagcg
     4621 cgcggaaaac atttaggttg acatgagacg agcgtctttt cagacgctaa acaacgagct
     4681 gtaaaaagga tccagatgaa taggttgagg cgtttccctt aaaaaatggc tgggttactc
     4741 acccggccaa aaagctactc cactgaagtt tcaagaaggt gcgtttggtt agccaccgta
     4801 cctttcaacc aaattgttga aaaacttaac tgctcgcgca acatctttgg catttggacg
     4861 gccttttgct atacctccaa tgatcttaaa cagtccatag gtgtcatagc ctttgcaccc
     4921 gtaatgcccg acaaactgcc catttctcag actaataatc tttttaaggt tgtgtatacg
     4981 gggctttacg ccaccgtacg tggatattac gaagaccttc ttttccatgg gcagatttct
     5041 ttcagcaaat tctattacgc tctgatgaag tttagagaaa taggtgcctg atgcaaagcc
     5101 tataaggtcg taaccggaaa gatcggcatc tagggttgtc gacgcgtcga ttaaagtaat
     5161 atcgctttga gtagctattt gatctaagag ctttttggtg tttccgtggt gcttggagta
     5221 atagatgata gctgttttca cctgcattcc cccattctcg atacggtcac ctgtgtcttg
     5281 tctctatcgc tcggttcccc ttttgcttgt acctattgta gcacgagatg agttgattgc
     5341 gaaggcaaag gcctcgacac ataaaggtca agctggtttt aaaagcgagg accccaatgc
     5401 actcacgatc attgaggtcc tcagaacatg aggaagtgtg ttggatctag ctttcggtaa
     5461 actgaactcc tccaggggta ttatcggaga aatcgttgac agccaaaatc tcttcttcgg
     5521 ttgtcatttc gtttccatct ttgtccttca cctttacgct ttgtgcgatc gagacaacac
     5581 catcacttgt cgtggaagag ttattggtca cgttattgtc gacgaacgtg atatttgcac
     5641 tcgttgcaaa catagcagcg ccgcctattg ctgcggtgtt tccgcctatg tcattgtcgg
     5701 tgatactgac tgaagagaac gagatataca tacctccacc ataagaggct tcattgttga
     5761 taattgtgca attggagatg gtgtttgcac cctgtccgtt ggacatccaa ataccagccc
     5821 cggtgaagct tgcatagttg tcgcttatta aagtgtcggt taacgtcaaa gtcgagttat
     5881 ttgagtaaat acccgcccca tgactacctg tattcccagt aattgcgcag ttgctgatca
     5941 cagctttgct gtactgtccg aggtaaatgc ctccaccata agcgtaatcc cctgacatgg
     6001 gaactccaga accgttggtt agggtaagcc ctgatatggt gacgtcggcg tactggtcga
     6061 tgagaatgca gggtcccttt ttatctccgt cgataaccgt tgccaaaatg gttagaggat
     6121 catctggatt ggtgcctcta atggttatat tcttctttcc tgtaaggtca aggttttcgt
     6181 tgtaagtgcc cggtgatacg acgatttcgt atcccggccc agctgcattg attgcagctt
     6241 ggatggtctt atactgttcg ggaaccctta gcgaggactc tttaaacaca gctcgtacct
     6301 gtctggactt gtctacgtaa accactaaag gattggttgt gcctgtagca tctagttccc
     6361 aatggctgaa aagccaacca tcatcgggaa ccgctttcag ttccacaaca gaaccgtact
     6421 cgtagacacc tctacctgca agcacggttt cctctacttt cccattcccc tgaactatta
     6481 cgtagataaa gtgtgtgtct tttgagaaca ccgctttgat gttcttcggt gagtccattt
     6541 ttacggtgat cacctgggag ttgccgtcga tgtcccctgt ccaatgcgca aacttccagt
     6601 tatccgccgg tattgcttgg agctcaacaa tagtgtcttt cttgtagcct ccggcttcag
     6661 gttcggggtt actgactagg cgtatgtact agaggaacga aacatcgttg tcgcttctta
     6721 agagcacgca gctttttggc cttgtccatt caagaatctt catgtgacac ggagattata
     6781 ctaaaataat cgtttgatat ttcagaaatt taaatagggc aaaagtcata tttgcggttc
     6841 tctggaaaat ggaagtgaag ttttgcacca taggacttgt tttcatgctc ttagcattgg
     6901 tggggggtta accagcttcg aataatcttc cacgaaagtt cctgaaac
//
LOCUS       DAOSDA010000028         6932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_7028_length_6932_cov_2.916243, whole genome shotgun sequence.
ACCESSION   DAOSDA010000028 DAOSDA010000000
VERSION     DAOSDA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7028_length_6932_cov_2.916243"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLW82_01220"
     CDS             <1..430
                     /locus_tag="PLW82_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45927.1"
                     /translation="TPVFEPRLLVVDEMSSVSVDMPLMIYGSVDGFVFIDFNKNGIFD
                     RDDIPLNFAQVLLNGSKNTLTNGSGRFIFDHIPLGTATLSVDPESLPPNTKAEEVQIV
                     LDEHRNDVYDLYIPVYVVENSSNNATNYLTNHKTTESEMN"
     gene            complement(526..1239)
                     /locus_tag="PLW82_01225"
     CDS             complement(526..1239)
                     /locus_tag="PLW82_01225"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOJ45928.1"
                     /translation="MEGKTSFVLILGVAMLLSALVLGLFFYNAQKDQSTVTVVGQAVQ
                     GFEADTVKWAITIEEETGPNDLALGYRQLEEGVRNLKEQLSSRGIDASEISLKTPYVY
                     KDYNYDLGVVTRLRFQQTVIVVTDELDVVEELAFNPVQLLEANVNIMNSNVEFYYSKI
                     DEMKKELVSLATRNAKERAEKMLEQTDVKLGKLISVRSGVFQITEPYSTEVSSLGIYD
                     TSTRKKQISVTASAVFAIK"
     gene            1436..2548
                     /locus_tag="PLW82_01230"
     CDS             1436..2548
                     /locus_tag="PLW82_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HOJ45929.1"
                     /translation="MEKQRISGEQLAFMVGAQLMGNVLLIDPASYAGHLGWVVSLTGG
                     LYALFLCWIYNSLSGKFPGISPTDYAVILFGDFLGKAVILSHFVLFMLSAMVSFNDFL
                     AFTNLGMPETPKIVIIICQAVVVMIALYLGLESIARTTVFIIVFSILSFVLSTVLALK
                     DMDFMLLLPLATDRPQEFWQSLFTVIGWPFSRLVLFLWFIPLVNNPKEAKKGLLRGVL
                     LGSSIVFFASLRNICIFGDGVTNQLFSAISFARNISIGANVMRVEIITSLTLNFIGFV
                     STTLFLYASVLCIAQMFNLRSHRTLILPLFVVVVLITSRLPLNIALLSWADRHFIRYF
                     LFIMGFVLPVIGVIRSFFVKEKQGPAKKNRSTKARR"
     gene            2553..4132
                     /locus_tag="PLW82_01235"
                     /pseudo
     CDS             2553..4132
                     /locus_tag="PLW82_01235"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
     gene            4129..5268
                     /locus_tag="PLW82_01240"
     CDS             4129..5268
                     /locus_tag="PLW82_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOJ45930.1"
                     /translation="MKKLLIALFCLILTFTSGCWSSYEIEELALASAVGVDYDGTKYN
                     LTIQIIKPEIVAEKTGTDPVTTIYSSGATMFDAIRNALSYSPKRLYWGYLQTMIFSER
                     ALEKGLACLIDFLSRDFEIRPIIWIAVTEKDPEELLKAKVSQDVPSGEGINAMIESQK
                     GLSKCVHSMLTQALICLGNEGRDFACALFGTTKMEDEEFMEIKGTALFRGEQYVGSID
                     TLESRGLLWLMDRIKSGIIVLKAPQDEKLALEILSSKTKIKTKVDGDKIGFEVTIEVV
                     SNLAEQTANLNPAHPDQWKELAEKQAQHVKDEAEKALRAIRNLGTDPVGFGKLLRAYH
                     PKVWYQVRDHWHDLLKTVPIAINVKSKLKSTGMILGDVAIYGSLE"
     gene            complement(5381..6664)
                     /locus_tag="PLW82_01245"
     CDS             complement(5381..6664)
                     /locus_tag="PLW82_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ45931.1"
                     /translation="MIRKLSFSVLVCVLMLAFSVGVIAEKITVWTWYDGTLGASFKQL
                     LQEDFVAKTGIEVELYTAPVADITTKLLLAVIGGDAPDVVMLYSNQVVELGVRGALHN
                     IVGMPELESTFEIMLPGLMRQLSYKSAVFALPGEVNWTWTYYRTDILQEVGAEVPNTW
                     DEMKSLSTKLKARDMDVYYDYQGDVAARSTGRLLPFVFQRGTDIYTEDGTASNLTDPR
                     VVEGFKELVSLFKDYKWPLENPGSTTFVDGATPIQIYQNWYYSVYLRYPQISGKWDIA
                     EIPGTVQSDGTIDRTNTGKMLTWSITSSSKNKEAAAKFLAWAVSPEFTSKFSQLGYDS
                     PEKWRLFFSTRDGLDRSGFPEEHMPMAQAALEKVKMQRSVVGGLVADRYIDFAFTTVV
                     LQNTSPEEALKTAAKDSTDEIQRKIKEFSRFIASL"
BASE COUNT         1689 a         1850 c         1606 g         1787 t
ORIGIN      
        1 caccccggtc tttgaaccca gacttctggt cgtagacgag atgagttccg tatctgttga
       61 tatgcctcta atgatttacg gcagcgtcga cggatttgtc tttatcgatt tcaacaagaa
      121 cggtatcttt gaccgcgacg atattcccct caatttcgcg caggttctcc tcaacggatc
      181 gaagaacacc ctgacaaacg gatcaggtcg gttcatcttc gaccacatcc ctttaggaac
      241 cgcaaccctt tctgtagatc cggaaagcct tcctccaaac accaaggcag aagaggtcca
      301 gatcgtcctc gatgagcaca gaaacgatgt ctacgatctc tacatcccgg tctatgtcgt
      361 cgaaaactcc tcaaacaacg cgacaaacta cttaacaaac cacaaaacca cagaaagtga
      421 aatgaactag aaggcagcca gccctctagt tcattttttg tccgcgtgct cgggaaaccc
      481 cgagtttttt tgtgtccgtt ttaagaagca aaaagcacct ctaccctact tgatggcaaa
      541 gacggcactg gctgttacac tgatctgctt cttcctggta gacgtatcgt agataccgag
      601 actggagact tccgttgagt agggttccgt aatctggaac acgccggagc gcactgaaat
      661 gagttttccc agcttcacgt cggtctgctc gagcatcttc tccgctctct ctttagcgtt
      721 cctagtcgct agagacacca gttccttctt catctcatcg atctttgagt agtaaaactc
      781 tacgttggag ttcatgatgt tcacatttgc ctctagaagc tgcaccggat tgaaagctag
      841 ctcttccact acatccagct cgtcagtgac gacgatcacc gtctgttgaa agcggagcct
      901 tgtgacaaca cctaggtcgt agttgtaatc cttgtaaacg taaggagtct tcaggctgat
      961 ttcagaagca tcaatccctc ttgaactcag ctgctctttg agattgcgca cgccttcttc
     1021 aagctgcctg tatcccagtg caagatcatt aggcccggtt tcttcctcaa tggtgattgc
     1081 ccacttgacc gtatctgctt caaaaccctg aaccgcttga ccgactaccg taacagtgct
     1141 ttggtctttt tgtgcgttgt agaaaaacag gcccaaaacc aaagcagata agagcatggc
     1201 tacccctaaa ataagtacaa aactagtttt gccttccacg ctgccacctc ctctaagcta
     1261 ctatagtttt cttcttcagg ttttatttcc ctctatgtaa aatcctggag tcctcgtccc
     1321 ctagttcttg gtactagggg ttttttcttt ccactaaagc ccgcttttta catactacca
     1381 ccacttgttg ttaaagataa aaaggaccct taaaggtggt gtaatcaagt tgctagtgga
     1441 aaaacagaga atcagcggtg aacagctggc ctttatggtg ggagcacagc tgatgggtaa
     1501 cgtgctgttg atagatccgg caagctacgc cggacatcta ggctgggttg tgtccctgac
     1561 aggaggtctc tacgcgctat ttctctgctg gatatacaac tccctctcag gcaagttccc
     1621 tgggataagc cctaccgact acgctgttat cctcttcgga gatttcctgg gcaaagcggt
     1681 catcctcagc cactttgtcc tctttatgct gtctgccatg gtcagcttca acgactttct
     1741 ggcttttacc aaccttggga tgcccgagac ccccaagatt gtgatcatca tctgccaggc
     1801 ggtggtggtc atgattgcac tctatttggg gcttgagtcc atcgccagaa caacggtgtt
     1861 catcatcgtc ttcagcatcc tgtcattcgt cctcagcact gtcctagcct taaaagacat
     1921 ggactttatg ctgcttcttc ccctagcaac ggatcgccct caagaattct ggcaatccct
     1981 gtttacggtg atcggttggc ctttcagtcg gctggtgctg tttctgtggt ttatccctct
     2041 ggttaacaac ccgaaagagg caaaaaaagg ccttcttcgg ggagtgctgc tgggatctag
     2101 catagtgttt tttgcatctt tgcgcaacat ctgcatcttt ggcgacggtg ttaccaatca
     2161 gctcttttct gccatttcct ttgcccggaa catcagcatc ggcgcaaacg tcatgcgcgt
     2221 cgaaatcatt acctccctaa ccctcaactt catcgggttt gtgtcaacta ccctgtttct
     2281 ttacgcaagt gtactatgca tcgcccaaat gtttaacctg agatcccacc ggacgctgat
     2341 cctgccgcta tttgtcgtag tggttctcat caccagtagg ctcccactga acatcgccct
     2401 gctatcttgg gcagacaggc acttcatccg gtactttctc tttatcatgg ggtttgtact
     2461 gcctgtaatc ggcgtgattc gctccttttt tgtcaaggag aagcaagggc ctgccaaaaa
     2521 aaacagatcg actaaagcca ggaggtgatc ccgtgctgcg ctggataaaa aacaagctgg
     2581 actcactgct gctctttcct caagaagacc ctgctccgaa actgccgtct cccacaaagt
     2641 ccctggacca gaagctaaac tacatcaaaa gccagtttag ggagagtccc gacgtctcca
     2701 ctagagagct gcgcataggt cccgggggta aaacccgtgc tgccctcttt tttgtggaag
     2761 gaatggtcga caaagaagcg ctcggaatca gagtgattcg ccccttagtt caagaggagt
     2821 tatataaaaa catcgaagat acgccactca gctacgtgga ggccatcgca aagcacctgc
     2881 tcctctcggt tgaagttaag accgaaaaaa agatgcatga gattgttgag tgcgtcctcc
     2941 aaggcgacac tgctctgttt atcgacgggt gcgaagacgc cttaatcctc gggcatcgca
     3001 aactgcaaac cagggccatc gaagaacccg attccgagcg aacaacccaa ggccctcgag
     3061 ctgggtttgt tgaggatgtc agaatcaaca ccgccctcct taggcagacc atctccagtc
     3121 ccaacttggt gttggagatg actacagtgg gcgtccaaac caaaactaag atctgcatcg
     3181 cctacctcaa agacattgtg cggccggaga tcgtggcaga ggtcaaaagg cgactgaact
     3241 cgtttaaaat cgacgccctg atcacctccg gcatgctcac agagctcatc tctgatgctc
     3301 cccactccat ctttcccgtt gtggcctaca gcgaaagacc ggacaaggtt gcaagcaaaa
     3361 tactctgcgg acgggttgcc attatcgtcg acggtacgcc ttttgttctc accgtccccc
     3421 acctttttat cgagaacttc caggtacctt cggactacgc cagcccctgg gtctttcaga
     3481 gttctgtgag gttgctgcgg ctgttctcgt ttttggtgag catcctcacc gtcccgttta
     3541 tgtagggatc acgtcgttcc accaggagct gttgcccaac aagctggcca tgaccattgc
     3601 cgctgccaga atggccgtac cgttttcggc gattgtcgaa gcactggttc ttgcattctt
     3661 cttcgagatc ctgcgggaag cttctctgcg gattccgggg gttgccggag gtgcggtcaa
     3721 cattgtaggt gccctggttg tgggagaagc tgcggtgaac gcaggcattg tcggggctcc
     3781 catggttatc gctatctccc tatctgctat cgctaacttc gttgtaactc cgctggcaga
     3841 gtcggcaact ctccttcgat acgtcttcct tctgctcgta ggttttatgg ggttctacgg
     3901 actcgcttat ggggtgatcg tcaccacact ctacctaacc tcactctact ccttcggcgt
     3961 cccctacctt tctcccctgt cgccggttat ccccgaccag ctcggcgaca ccgtcattag
     4021 aactcccatc tggaacaggg tgttccgtcc ccggcagttt gccaaagaca gaaaacgcgc
     4081 tgagttccgc ctcacccacc aaaaacagaa cactaaggat gagaaccatt gaaaaagctg
     4141 ttgatcgctc tcttttgcct tatacttact tttacgtctg gctgctggag ttcatacgag
     4201 attgaagaac tagccttagc ctcagctgta ggtgtcgact atgacggcac taagtataat
     4261 ctaaccatcc agatcattaa accggagatc gtcgccgaaa aaaccggaac cgacccggta
     4321 actaccatct attcctcagg tgcaactatg tttgatgcca tcagaaacgc cctatcatac
     4381 agtcccaaac gactctactg gggatacctc cagactatga tcttcagcga gcgagcattg
     4441 gaaaaggggc ttgcctgcct catcgacttt ctgtcaagag actttgagat taggcctatc
     4501 atctggattg ccgtcacgga aaaagaccca gaagaacttc taaaagccaa agtctcccag
     4561 gacgttcctt ctggagaggg catcaacgct atgatcgaga gccaaaaggg tcttagcaag
     4621 tgcgttcact ccatgctgac acaagctctg atctgcctgg gtaatgaagg acgggacttt
     4681 gcctgcgctc tctttggaac cactaaaatg gaagacgaag agttcatgga aatcaagggt
     4741 acggctctgt ttcgcggcga acagtatgtt ggcagcatcg atactctcga gtccagaggg
     4801 ctcctctggc tgatggaccg cataaaaagc ggcatcatcg tcctgaaagc tcctcaggat
     4861 gaaaaactcg ctctggagat cctatcttca aagaccaaaa tcaagaccaa ggtcgacgga
     4921 gacaagattg gctttgaggt gacaattgaa gttgtcagca atcttgccga gcagacggca
     4981 aacctcaatc cggcccaccc tgaccagtgg aaggaacttg ccgaaaaaca agcacaacac
     5041 gtaaaggatg aagcggaaaa agcgcttcgt gccatcagga acctgggtac agatccggtt
     5101 ggcttcggca agctcctcag agcatatcac cctaaggtct ggtaccaagt ccgggatcac
     5161 tggcacgacc tgcttaagac ggtgcccata gccatcaatg tgaaaagcaa attgaaatcc
     5221 accggcatga tcctcggtga tgtggcaatc tacggtagct tagagtaagc tatcacctgc
     5281 aaaaaagacg ggaagtctcc ccgtcttttt tattgcatag ttagttctgt ttttcggtta
     5341 aggaaaatct cctaacggag agtctccttg gaacgcgacc ctatagactt gcaatgaacc
     5401 tgctgaactc tttaatcttc ctttgtatct catcggtaga gtccttggct gctgtcttga
     5461 gggcttcttc agggcttgtg ttttgcagca ctacggtggt aaacgcgaag tcgatatagc
     5521 ggtcggctac taaccctcca acaacagacc tctgcatctt cactttttcc agagctgctt
     5581 gagccattgg catatgttct tccgggaaac cggagcggtc cagaccgtct ctggttgaga
     5641 agaagaggcg ccatttttcc ggcgagtcgt aaccaagttg cgagaacttg gacgtgaact
     5701 ccggagaaac tgcccaagct aagaacttag cagcagcttc tttatttttc gaagacgagg
     5761 tgatcgacca ggtcagcatc ttaccggtgt tagttctatc gatggtgcca tcgctttgaa
     5821 cggtgccggg aatctctgca atatcccact tcccgcttat ctgtgggtat cttaaataaa
     5881 ctgagtagta ccagttttga tagatctgga taggcgttgc accgtccaca aacgtagtac
     5941 taccggggtt ctcaaggggc catttgtagt ctttgaaaag gcttaccagt tctttgaagc
     6001 cttcgactac ccttgggtcg gttaggttgg aagccgtacc atcttcggtg tagatgtcgg
     6061 ttcccctctg gaagacaaac ggcagaagtc ttccagttga gcgggctgca acatcccctt
     6121 gatagtcgta gtaaacatcc atgtctctgg ctttcagctt agtggacagg gacttcatct
     6181 cgtcccaagt gttcggtact tcagcaccga cttcttgcag gatatctgta cggtagtaag
     6241 tccatgtcca gttgacttca cctgggaggg cgaaaaccgc actcttgtag gaaagctgtc
     6301 tcataagacc aggaagcatg atttcaaagg tagactcaag ttcgggcatc cctacgatgt
     6361 tgtggagagc accgcgaacg cccagctcta ccacttggtt ggagtagagc ataactacat
     6421 cgggggcgtc tccgccaata acagccaaaa ggagcttagt tgttatgtct gccacaggtg
     6481 ccgtgtaaag ttctacttct ataccggtct tggctacaaa gtcctcctgg agaagctgtt
     6541 tgaatgaagc gcctaaagtt ccgtcgtacc aggtccaaac ggtaatcttc tcagcaatta
     6601 caccgacact aaaggctagc atgagcacac aaaccagaac cgaaaaagac agcttacgga
     6661 tcatagttgt tcccccttta ggtactgtga gtttctatca agagcgttcg cgaagcaaaa
     6721 agcggctcct ccagaagggt tataattttt ttatttggtt gctccaagag aaatttagaa
     6781 gtgtttcata actccccgca gaactccaaa aaccacaccc tacaatctac tcatttccaa
     6841 atccactggc tgtcaaaaga tcagtatccc tagatgaact ctagcgtgta aagacgaact
     6901 cttcgctggg ctccgacagg tttccggcaa ag
//
LOCUS       DAOSDA010000029         6927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_7032_length_6927_cov_3.341676, whole genome shotgun sequence.
ACCESSION   DAOSDA010000029 DAOSDA010000000
VERSION     DAOSDA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7032_length_6927_cov_3.341676"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLW82_01250"
     CDS             <1..677
                     /locus_tag="PLW82_01250"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOJ45932.1"
                     /translation="LFFAEADLKEVSEIFKKRMDGERLTGKEVDQFKTYTLTRLAAMY
                     HKKDWAMQLHIGATRNNNTRMFRLLGPDSGYDSIANPLCADKLARLLDGMEVSGQLPK
                     TILYSLNANDNNVLAAMAGNFQSGELPGKVQFGAAWWFNDSLSGIEDQLETLANIGLL
                     SAFVGMLTDSRSFLSLIRHEYFRRILCNMLGSWVEEGKAPKDMKLLSTYVERICYLNA
                     KRYFSL"
     gene            706..2115
                     /gene="gndA"
                     /locus_tag="PLW82_01255"
     CDS             706..2115
                     /gene="gndA"
                     /locus_tag="PLW82_01255"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019378044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HOJ45933.1"
                     /translation="MAKQQIGVIGLAVMGKNLALNIESRGYSVAVYNRSPERTEEFME
                     NEAKGKNFAAAYSLQELADSLEKPRRIILMVKAGSPTDETIKALKPYLEPGDILIDGG
                     NTYFEDTIRRNKELSDAGIRFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPIFEAI
                     SAKVEGEPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAEAYAILKDLLGLSAEELHQV
                     FSSWNQGELNSYLIEITADIFKKIDDETGKPLVEVILDSAGQKGTGKWTSKNALDLGV
                     PLPLITESVFARFISALKEERVAASRILPGPDAKEFTGDKEAMIEAIRKALYMSKICS
                     YAQGFAQMREASEFYNWNLDYGKIAMIWRGGCIIRAQFLQKIKEAYDKSPDLKNLLLD
                     DYFNEIVREYHTALREVVAISVKHGIPVPAFSSAIAYYDSYRSENLPANLIQAQRDYF
                     GAHTYQRKDKEGIFHTQWF"
     gene            2325..3335
                     /locus_tag="PLW82_01260"
     CDS             2325..3335
                     /locus_tag="PLW82_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ45934.1"
                     /translation="MLKVAVIGLGDVSGIHLEAIRNSSEAELVAVCDIDESRKEAAPD
                     VNFYTDYLEMLDKEDLNCVHVCLPHYLHFPVTKACVEKGIHVYLEKPMTKDVYEGVEL
                     VEFCRKHPNVKVCVSLQNRFNDSFVEMQRIIDSEELGKVVGIKGLVVWNRPKAYYDVK
                     PWRGLMKYAGGGVMINQAIHTMDQMQVIGGEIESIRGTVDQLVNYGYEVEDTATAHIR
                     FKSGATGVFFATTTYTNNSSVELQVILEKGKLTIKDFMLFITDSDGNKQPIKEDAKLP
                     GTKFYYGASHNVLIGKFYKAIIENTDDYVHVEDGLITMEMIDAIYKSSNLKIPISMEV
                     YR"
     gene            3332..4159
                     /locus_tag="PLW82_01265"
     CDS             3332..4159
                     /locus_tag="PLW82_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ45935.1"
                     /translation="MSRAKICVQLYTLRREMARLGVYETLKKLHELGFTSVEVSALEM
                     TEANVSQLKKASEDFGMEIAALSAGENELKDLDKIVSDCKALGSRYVRVGMIPFRIMG
                     YKDKAMEFIRDLEDAAERLEKEGIKLYYHNHHYEFQKYDGAYLLDIMRERTEKLGFEL
                     DVHWIQRGGEDPIRIIKRFVGRVDLLHLKDYRIGPVSFTDEELKEQKVWDKLANIVQY
                     AELGEGNLDLQGIVETGLECGAKYLIIEQDETYDKDPFQSLKISKEALEKLGYGDLL"
     gene            4189..5457
                     /locus_tag="PLW82_01270"
     CDS             4189..5457
                     /locus_tag="PLW82_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOJ45936.1"
                     /translation="MGILEELLKDVPIPKVAKVRQVFDSEKLEDPEGELRLQLGLDKF
                     KGKIEPGAKVAIAVGSRGINKIAELTAVTVSFLKEQGAEPFIVPAMGSHGGATAEGQK
                     EVLAHLGITEESAGCRILSSMDVVQVGELPNGLPVYVDKFAYEADGIVVINRVKPHTA
                     FRGKVESGIMKMISIGLGKQKGAEATHQLGFKHMAEYVPMMANVILNKLPFLFAVATV
                     ENAYDQVAKVEVLLPEEIEEREPELLKLAKSLMPKLYFNQLDVLVIDEIGKNISGDGM
                     DPNITGRYPTPYAWGGPEVTKMVVLDLTKDSKGNANGVGTADFTTKRLADKMDFAATY
                     ANGLTSTVVGPTHLSTVLPSDKLAIQAAIKTSNILDFTKVRMVRIKNTLKLGEIEVSE
                     ALLEEVQKEPNLELVSELYELSFDEEGNLF"
     gene            5489..6259
                     /locus_tag="PLW82_01275"
     CDS             5489..6259
                     /locus_tag="PLW82_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ45937.1"
                     /translation="MKDMFDLTGKKAVAFGGNSTLGSAMAKVFAAYGAEVAIVGRNIE
                     TATQVAEEIVQDGGKAKAFQADVSVLSDITRVADEIKEWTGGWDILLNAPGKNSATPF
                     FDLTPEEWDDIMSVNLKGLVFTIQTFAKAMIEQKRKGSIINISSVSSGPPLSRVFTYS
                     VSKAGVNSVTQFLARELAPYGIRVNAIIPGFFPAEQNRRILDEARVDSIMRHTPMKRF
                     GEPDELQGAALYLASDKASGFVTGSLLRVDGGFGAMTI"
     gene            6311..>6927
                     /locus_tag="PLW82_01280"
     CDS             6311..>6927
                     /locus_tag="PLW82_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOJ45938.1"
                     /translation="MVLQDKYVIGLDLGTTSIKAVLFTTGGRVVMTTEVAVETLYPDP
                     LFSEQNPEEIDAKARKAVRNILQNPEVVDLGVEKNLIGIGISSAMHSLILTDKNYSPL
                     SNMLIWSDKRSRKQADSLLEEGTGFEIYRRTGTPIHPMTPLCKLLWMKETGYEAFRKA
                     AYVMTGKDYLAAKWSGRAVIDYGMASSTGLFDLQSLDWDDEALRL"
BASE COUNT         1927 a         1646 c         1869 g         1485 t
ORIGIN      
        1 tcctgttctt tgcagaagca gatctcaagg aagtctcgga gattttcaaa aagaggatgg
       61 acggagaaag actcacgggg aaggaagtag atcagttcaa aacctacacc ttaaccagac
      121 tagctgctat gtaccacaaa aaagactggg ctatgcagct ccacatcggg gctacgagaa
      181 acaataacac caggatgttc aggttgcttg gcccagacag cggttacgat tccatcgcaa
      241 atcccctctg tgcagacaag ctggcaaggc tccttgatgg catggaggta agcggtcagc
      301 tgccaaagac catcctctac agcctaaacg ccaacgacaa caacgttctt gcggcgatgg
      361 ccggaaactt ccaaagtggc gagctgccgg gtaaagtcca gttcggtgct gcctggtggt
      421 tcaacgacag cctctctggc atcgaggatc agctggagac cctggcaaac atcgggcttc
      481 tcagcgcttt tgtgggcatg ctcacagact ccagaagctt tctctcgcta attaggcacg
      541 agtacttccg cagaatcctc tgcaacatgc tgggatcctg ggtggaagag gggaaagccc
      601 cgaaggacat gaagcttctt tcgacctacg ttgagcgaat ctgctaccta aatgcaaaaa
      661 ggtatttctc tttatagatc tcatgaaaaa gtgaggaatt gaagtatggc aaaacagcaa
      721 attggcgtaa tcggtcttgc cgtaatgggc aagaacctcg ccctgaacat tgagtcgaga
      781 ggttattctg ttgcagtcta caaccgctcg cctgagcgga ccgaagagtt catggaaaac
      841 gaagctaaag gaaagaactt cgccgctgcc tacagcctcc aggagctggc agactctctg
      901 gaaaagcccc ggaggatcat cttgatggtt aaagcgggaa gcccgacaga cgagactatc
      961 aaagctctaa aaccctactt ggagcccgga gacatcctga tcgatggtgg aaacacctac
     1021 tttgaagaca ctatccgccg caacaaagag ctctcagatg cggggattcg cttcatcgga
     1081 acaggcgttt ccggaggcga agaaggagcc ctcaaaggtc cctcgatcat gccaggagga
     1141 cagaaagaag cctatgaact ggtagcgcca atcttcgaag ccatctctgc aaaagttgaa
     1201 ggagagccat gcgtcaccta catcggacct gacggagcag ggcactacgt aaagatggtt
     1261 cataacggca tcgagtacgg cgacatgcag cttatcgccg aagcctatgc cattctcaaa
     1321 gaccttttag gtcttagtgc cgaagaactg catcaggtct tctcctcatg gaaccaagga
     1381 gagctgaaca gctaccttat cgaaatcact gcagatatct tcaagaaaat cgatgatgaa
     1441 acaggcaagc ctcttgttga ggtgatcctg gactctgcag gccagaaagg tacagggaag
     1501 tggaccagca aaaacgcttt ggacctaggc gttcccctgc cgctcatcac cgagtcggtg
     1561 tttgcacgct tcatctcggc gctaaaagaa gaaagagttg ctgcaagccg gattctcccc
     1621 ggacctgatg caaaggagtt taccggcgac aaagaagcaa tgatcgaggc gatcagaaaa
     1681 gccctctaca tgagcaaaat ctgctcgtac gctcaggggt ttgcccagat gagagaggct
     1741 tccgagttct acaactggaa cctggactac ggcaagatcg ccatgatctg gcgtggcggc
     1801 tgcatcatca gagctcagtt cctgcagaag atcaaagaag cctacgacaa aagccccgat
     1861 ctgaaaaact tgctactcga cgactacttc aacgagatcg tcagagaata ccacacagca
     1921 ctccgtgagg ttgtagcaat ctcggtgaag cacggcatac cggttccggc gttttccagt
     1981 gctatcgcct actacgacag ctacagatcg gaaaatctcc ctgcaaacct cattcaagcc
     2041 cagagagact acttcggggc ccacacctat cagcgcaagg ataaagaagg aattttccac
     2101 actcagtggt tctagaccca ggagcgttcg tttagatagt aagcggcagt ttgctgcata
     2161 aactgccgct catttttgcg gaattatgct ggggttttga gaggattttt gagatagagg
     2221 agtaagtcta tcttggatac cgatctggct tgtttaacga gctagatgca aatacaaaac
     2281 acaataaaaa actgatgggc gcgtaacaaa aggagagacg aggcgtgtta aaagtagctg
     2341 taattggact tggagacgtt tctgggattc accttgaggc aatcagaaac agctcggagg
     2401 cagagcttgt ggccgtctgc gacatcgacg aaagcagaaa agaggcagct ccagatgtga
     2461 acttctacac cgactatctg gaaatgctag acaaagaaga tttgaactgc gtacacgtgt
     2521 gccttcccca ctacctgcac ttccctgtta ccaaggcgtg tgtggaaaag ggcatccacg
     2581 tgtacttgga aaaacccatg accaaagacg tctatgaagg agtggaactg gtagagttct
     2641 gcaggaagca tccaaacgtc aaggtctgcg tcagtttgca gaacaggttc aacgattcct
     2701 tcgtagagat gcagcgaatc atcgactcag aggagctggg gaaggttgta ggtatcaaag
     2761 gccttgtagt ttggaacagg ccgaaagcct actacgatgt caaaccctgg cgtggtctga
     2821 tgaagtatgc cggcggtggt gtcatgatta accaggccat ccataccatg gaccagatgc
     2881 aggttattgg cggcgagatc gaatccatca ggggtaccgt agatcagctg gttaactacg
     2941 gctacgaagt ggaagacact gcaacagccc acatcagatt taagagcggc gctaccggag
     3001 tgttctttgc caccactacc tatactaaca actcctctgt agagcttcag gtgatcctgg
     3061 aaaaaggtaa gctaaccatc aaggacttca tgctttttat tactgattcc gatggcaata
     3121 aacagcccat caaagaagac gcaaagcttc ctggcaccaa gttctactac ggcgcaagtc
     3181 acaacgtgtt gataggtaag ttctataagg ccatcattga gaatacagac gactacgtcc
     3241 acgtagaaga cggactgata actatggaga tgatcgatgc catctacaag tcgtccaact
     3301 tgaaaatccc tatttcaatg gaggtatata gatgagcaga gcaaagatct gcgtccagct
     3361 ctacacgctg cgacgggaga tggcaaggct cggcgtatac gaaaccttga agaagctcca
     3421 cgaactgggc tttacctcgg tggaggtatc ggctttggag atgacagaag caaacgtctc
     3481 ccagctcaaa aaagccagtg aagacttcgg tatggagatt gctgccctct cggcaggaga
     3541 aaacgagcta aaggatctgg ataagattgt atccgactgt aaagctctgg gctcccgcta
     3601 cgtccgagtt ggcatgattc cctttcgcat tatggggtat aaggataagg caatggagtt
     3661 tatccgggat ttggaagatg cggcagagcg tttggagaag gaaggcatca agctctacta
     3721 ccacaaccac cactacgagt ttcaaaagta cgatggtgcg tacctcttgg acatcatgag
     3781 ggaacgcacc gagaagctcg gtttcgagct ggatgtccac tggattcagc gaggcggcga
     3841 ggatccgatc aggatcatca aaaggttcgt tggcagagtg gatcttctgc acctcaaaga
     3901 ctaccgcatc ggacctgtct cctttaccga tgaagagctg aaggagcaga aggtctggga
     3961 taaactggca aacatcgtcc aatatgctga gctcggcgaa ggcaacttgg atcttcaggg
     4021 aatcgtagaa acgggactag agtgtggcgc gaaatacctc atcatcgagc aggatgaaac
     4081 ctacgataaa gacccgtttc agagcctcaa gatctccaaa gaagccctgg aaaagctcgg
     4141 ctacggcgat ctactttaaa agtactcttt agaaggagca gattttctgt gggcatatta
     4201 gaagagctgc tgaaagatgt gccgatccca aaagttgcaa aggttaggca ggtctttgac
     4261 agcgaaaaac tcgaagaccc agaaggcgaa cttcgcctcc agctgggtct ggataaattt
     4321 aagggtaaga tcgaacccgg tgcaaaagtg gccattgcag tggggagcag gggcatcaac
     4381 aagatcgccg aacttactgc ggtcactgta agctttctga aagaacaggg cgcagagccg
     4441 ttcatcgttc cggccatggg aagccacggc ggcgccactg ctgaaggaca gaaggaggtc
     4501 cttgcccacc tgggcatcac cgaagagagt gccggttgca ggatactctc ttccatggat
     4561 gtggtgcaag tgggtgagct acccaatggg ctcccggtat atgtagacaa gtttgcctat
     4621 gaggcagacg ggatcgttgt gattaacagg gtaaagcccc atacggcatt caggggcaaa
     4681 gtcgaaagcg gcatcatgaa gatgatcagc atcggactgg gcaagcaaaa aggcgccgaa
     4741 gctacccacc agctgggctt caagcacatg gcagaatacg tgcccatgat ggcaaacgtg
     4801 atcttgaata agctaccgtt cctctttgcc gttgccacag tggaaaacgc ctacgatcaa
     4861 gttgccaagg tggaagtgct gcttccagag gagatcgaag aaagagagcc ggaactcctc
     4921 aagttggcaa agagcttgat gccgaagctc tacttcaatc agctggatgt gctggttatc
     4981 gatgagattg gaaagaacat cagcggcgac gggatggatc ccaacatcac cggacgctat
     5041 cccaccccat atgcttgggg aggaccggag gtcaccaaga tggtggtctt ggatctaacc
     5101 aaagactcca aaggaaacgc caacggtgtg ggaactgctg actttaccac caagcgcttg
     5161 gcagacaaga tggactttgc agctacctat gccaacggcc tgacttccac tgttgtgggg
     5221 ccgacgcacc tttccacagt ccttcccagc gacaaactgg cgattcaggc agcaattaaa
     5281 accagcaaca ttttggactt taccaaggtg cgtatggtgc ggatcaagaa cactttgaag
     5341 cttggagaga tcgaggtttc agaagccctt ctggaagaag tgcagaaaga acccaacctg
     5401 gagctggtct cggagctcta cgaactctcc tttgacgaag aaggcaacct gttttagata
     5461 gcagtttact tttaaggagg cgaagcttgt gaaggacatg tttgatttga ctgggaagaa
     5521 agcagtagcc tttggcggaa acagcacctt aggaagcgcg atggcaaaag tctttgcagc
     5581 ctacggtgcg gaggttgcta ttgtagggag aaacatagaa actgcaacgc aggttgcaga
     5641 ggaaatagtt caagacggcg ggaaggccaa agcctttcag gccgacgtta gcgtccttag
     5701 cgacatcacc agagttgccg atgagatcaa ggagtggacc ggaggttggg acattctcct
     5761 gaacgctccc ggcaaaaaca gcgcaacacc gtttttcgat ctgaccccag aggagtggga
     5821 cgacatcatg tcggtgaacc tgaagggtct ggtgttcacc attcaaacct ttgccaaagc
     5881 aatgatcgag cagaagagaa agggcagcat catcaacatc tcttcggttt cgtcgggacc
     5941 gccgctgtcc agggtattta cctactcggt ctccaaggct ggggtaaaca gcgtaaccca
     6001 gtttttagca agggaacttg ctccctacgg tatcagggtc aatgcgatca ttcccggatt
     6061 cttccctgct gagcagaacc gtaggatctt ggatgaggcc agagtagatt cgattatgcg
     6121 gcacactcca atgaagcgct tcggtgagcc cgatgagttg cagggtgcgg cattgtatct
     6181 ggcgtcggat aaagcctcgg ggtttgtaac tggatctctc ctcagggtag acggcggttt
     6241 tggagccatg acaatctaaa gctgaaaagg ccttgagctc cggctcaaag ccttttttta
     6301 aggtaaggtg atggttttgc aggataagta tgtaatcgga ctggacttgg gaactacaag
     6361 catcaaggct gttttgttta ccacaggtgg cagagttgta atgacgacgg aggtagctgt
     6421 agaaaccctc taccccgacc cgctgttctc ggagcagaat ccggaggaga tagatgccaa
     6481 agcgaggaaa gctgttcgca acattctcca gaatcctgaa gtggtggact tgggcgtaga
     6541 gaagaatctc atcggcattg gcatctcttc tgccatgcac tcgttgatcc tgacagacaa
     6601 aaactacagt cccctctcca acatgctgat ctggtcggac aaaaggagta gaaagcaggc
     6661 agactccctt ctagaagagg gaacgggctt tgagatctac agaaggacgg gaacgccgat
     6721 ccatcccatg acgcctctct gtaaacttct ctggatgaag gaaacgggtt atgaagcgtt
     6781 caggaaagct gcttacgtga tgactggtaa agactatctt gcagccaaat ggtctgggag
     6841 agctgtaatc gactacggca tggcgtcttc aacaggactc tttgacctcc agagtttgga
     6901 ttgggacgat gaggccttga ggctctg
//
LOCUS       DAOSDA010000030         6546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_7536_length_6546_cov_3.541519, whole genome shotgun sequence.
ACCESSION   DAOSDA010000030 DAOSDA010000000
VERSION     DAOSDA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7536_length_6546_cov_3.541519"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /gene="frr"
                     /locus_tag="PLW82_01285"
     CDS             <1..485
                     /gene="frr"
                     /locus_tag="PLW82_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOJ45939.1"
                     /translation="TLRTGRANPALLDRVEVEAYGTKMPLNQVAIVSVPEPRMIVVQP
                     YDKSTINAIDKAIRIADLGFNPASDGNVLRIAIPPLTEERRKELVKVAKKICEDMRVA
                     VRNVRRDSNEALKKLEKDKEITEDDLKKGQNEVQKLTDDYIAKIDGILADKEKEIMEV
                     "
     gene            489..674
                     /locus_tag="PLW82_01290"
     CDS             489..674
                     /locus_tag="PLW82_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45940.1"
                     /translation="MERYLGRPLSDLLQREPNAPFVVKRLNAENTGREMILRIRRLDQ
                     VIEITTAFEVPTDTGLS"
     gene            694..1413
                     /locus_tag="PLW82_01295"
     CDS             694..1413
                     /locus_tag="PLW82_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOJ45941.1"
                     /translation="MPQRPALTVVPRHVAIIMDGNGRWAKKRGLPRVAGHREGAKTVR
                     MVVRTSGEVGVEVLTLYAFSTENWRRPEEEVSYLMNLLISYLKKELDELCRENVRLRA
                     IGDISNLPREVQQALLEAIQKTDNNTGMILNLALNYGGKDELVRAIKRIYRDVESGKF
                     PIDSVTPDLIDTYLDTASQPDPDLIIRTSGEMRLSNFLLWQGAYSELWFTDTLWPDFT
                     EEEYLKALVDYGKRGRRFGNV"
     gene            1426..2187
                     /locus_tag="PLW82_01300"
     CDS             1426..2187
                     /locus_tag="PLW82_01300"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOJ45942.1"
                     /translation="MKTRVLTAVVGIPLLLIVASLGGWWFFTLGLLAAVIALYEFHGF
                     AAGLGSNLSLSALILGGVASYLCVYFKLYNLLVVLFVALLILELFRTRSVLKNGSLLL
                     MGSVYAGGLFAFLPLLRDLSFGYIILLLLITWGSDTIAYFWGIKYGRNKLWPAVSPKK
                     SWEGAFGGLVGGLLGGLVAGLFGIVPTLYGALIGVGGSVVAQVGDLLESAMKRECGIK
                     DSGFILPGHGGVLDRCDSMIVLAPFLYLAISWILR"
     gene            2195..3355
                     /locus_tag="PLW82_01305"
     CDS             2195..3355
                     /locus_tag="PLW82_01305"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006556675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOJ45943.1"
                     /translation="MKTVAIIGSTGSIGTQALDVLRDLDHEFQVVALAANTSVDKLYA
                     QIGEFQPRLVAVQDPKAREELEARLTGSIAQVVGGDKPLETLVLEADADITIVSVVGA
                     AGLLPALAAARLGKRLALANKESLVLGGELLLAECSQHGTELIPIDSEHSAIFQCLLG
                     QDIDAVGKIVLTASGGPFLHVPAEKMRVAALEDALAHPTWDMGGKITIDSATMMNKGL
                     EVIEAHYLFGIDYDRIEVLVHPQSIIHSYVEYNDGAIIAQLGIADMRVPIQLALTYPQ
                     RKPRSERLNLVGKTLEFFAPDLEKFGCLRLAYEAGRAGGDRPVVLNAANEVAVEHFLK
                     RKITFGGIYEVVLETLERFPTRSASTLSELLEVDKLVREFAVGLIETGRWRT"
     gene            3343..4428
                     /locus_tag="PLW82_01310"
     CDS             3343..4428
                     /locus_tag="PLW82_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOJ45944.1"
                     /translation="MENIIVQLITIVLVFAVLVIVHELGHFLVAKALGIWVYEFSIGF
                     GPVLFKKKTKETQYSIRAFPFGGFVSVAGMDGAADAERGDVPPERLYTNKPAWGKVAT
                     IAAGPLMNIVFAMVISVLIIGMSGRQVPVVGQSVPGSPAERAGIQSGDEIWAVDNMNL
                     YNQGQFLQLIASSQGQSLQLKVKRDDTFLTFEVSPEFNEELGRSIIGVDGLTYKQIPL
                     NALNLLVSGWDNTKIMIVTLFRGLKAMITGKVEADVASPIGMVQIIGETASYMEVNFL
                     LGLLNVMYLAVFLSVNFAIVNLLPVPILDGGWILLIIIELIRRKPLTERQKAIAQMTG
                     LVFIAGLFLFATYSDFSRLGWLSFLER"
     gene            4436..5515
                     /gene="ispG"
                     /locus_tag="PLW82_01315"
                     /gene_synonym="gcpE"
     CDS             4436..5515
                     /gene="ispG"
                     /locus_tag="PLW82_01315"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014423756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOJ45945.1"
                     /translation="MKVREVKIGPLQLGGDAPVLIQSMTNTKTEDVKATLEQINALCE
                     VGCDFVRVAVPSKDTIKAFKEIVKSAPCPIVADIHFDWRLAVEAIEAGASKVRVNPGN
                     LGGLDKLREVARAASGRSAIRVGVNSGSIGRDYLAKIQSGEITKAEAMTRSAKDYVDF
                     LYDSGFYDVVVSLKASDIPTTLSVNRTFRQICDSPLHLGITEAGTAFSGTIKSAVGLG
                     VLLLEGIGETIRVSLTAPPTEEVKLAKEILKATGRRKMGPTIISCPTCGRTEIDLYTL
                     ATQVEKLTESIPEDITIAVMGCVVNGPGEAREADIGVAGGRGAGVIFVGGEIVEKVKE
                     EDLFERFTVHLERLLQEKKQKDASK"
     gene            5563..6195
                     /locus_tag="PLW82_01320"
     CDS             5563..6195
                     /locus_tag="PLW82_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ45946.1"
                     /translation="MKIAAIVPAYNEEDNISKVLVPLGFAKEQGYIHQVLVVCDGCED
                     RTADVAKSCGAEVLELNPNRGKGGAMLEGAMFTDADMLVFVDADLIGLTPEHISSLTK
                     PLLEGRAKMSMGIFGHGRFSTDAAQKVAPFLSGQRAIFREDFLKIPNLATSRYGVEIE
                     ITKYSKKHRWKVQRVVWDQMSQVMKEEKLGFFGGLKVRLRMYWEIIKASM"
     gene            6293..>6546
                     /locus_tag="PLW82_01325"
     CDS             6293..>6546
                     /locus_tag="PLW82_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45947.1"
                     /translation="MATYIIEPNEEKCRKLLPDLPVRIVRVVVDEERGRLEVDVRSHT
                     ELDFKTKQKIRDAIKSHIPEARTINVCCWLEDSDYTGDKP"
BASE COUNT         1709 a         1484 c         1823 g         1530 t
ORIGIN      
        1 gcacgttgcg cacgggcagg gctaatcctg cgctgctcga ccgcgttgag gtagaggcct
       61 acggtacgaa gatgcctctg aatcaggtgg cgatagtttc cgtgcccgaa ccgagaatga
      121 tcgtcgttca gccttatgac aaatccacca taaatgccat cgataaggcg attcgcattg
      181 ccgacttggg gttcaacccg gcatcagacg gcaatgtctt gagaatagcc attcctccgc
      241 tcacagaaga acgccgcaaa gagctggtta aggtggccaa gaagatctgt gaggatatgc
      301 gagttgcagt gcgcaacgta cgcagggaca gcaacgaagc actgaaaaaa ctggagaaag
      361 acaaagagat caccgaagat gatctcaaaa aaggtcaaaa cgaagtgcag aagttgaccg
      421 acgactacat tgccaaaata gacggcattc tagcagacaa agagaaagag atcatggagg
      481 tttaggtctt ggaacgctat ttgggacggc ctcttagcga cttgcttcaa agggagccaa
      541 acgctccctt tgttgttaag cgcttgaatg cagagaatac aggtcgggag atgatcctgc
      601 ggattcgcag gttggaccaa gtaatagaga taaccacggc tttcgaagtg ccgacagaca
      661 ccggtctttc gtagtctagt tagggggatt tttatgccgc agagacctgc attaaccgta
      721 gtaccgagac atgtggccat cataatggat ggaaatggca ggtgggccaa gaagcgaggt
      781 ctgcctcggg ttgccggcca ccgtgaggga gccaaaacag tcagaatggt ggttagaacc
      841 agtggcgaag tcggcgtcga agtccttact ttgtacgctt tttccaccga aaactggcgg
      901 cgtccagagg aggaagtcag ctatctgatg aacctgctca tcagctacct taaaaaggaa
      961 ctggacgaac tgtgcagaga aaacgtaagg ctcagggcta taggcgacat ttccaacctt
     1021 ccgcgggaag tgcagcaggc tttgctagaa gccatacaaa aaaccgacaa taacaccggt
     1081 atgattctga acctggcttt aaactacggc gggaaagacg aactggtgcg ggcgataaag
     1141 cgcatctaca gggacgttga atcaggcaaa tttcccatag acagtgttac acctgatttg
     1201 atagatacat acttagatac cgcatcccag cccgatcccg acctgattat ccgaaccagc
     1261 ggagaaatgc gcctatcaaa ctttttactc tggcagggag cctattcgga actatggttt
     1321 accgatactc tttggccgga ttttaccgag gaagaatacc taaaggcgct tgtagattac
     1381 ggcaagagag gtagacgctt tggaaacgta tgaggagggt tttctatgaa gacgagggtt
     1441 ttgacggcag tggtgggaat tcccctacta ttgatagttg caagtttggg gggctggtgg
     1501 tttttcaccc tggggcttct tgcggccgtc atcgcgctgt atgagttcca cgggtttgcc
     1561 gcaggtttag gcagcaacct aagcctttct gctctgattc taggcggagt tgcctcatac
     1621 ctttgcgttt atttcaagct gtacaatctg ctagtagttc tttttgtcgc ccttctgata
     1681 ctagagctgt tccgcacaag atcggtactt aaaaacggct ctctcctcct tatgggcagc
     1741 gtctatgcag gaggcctgtt tgccttcctg ccgctactaa gagatctgag ctttgggtac
     1801 atcatcctct tgctcttgat tacctgggga agcgatacaa ttgcctactt ttggggtatt
     1861 aagtacggta gaaacaagct ttggccggca gtttcgccga agaagtcctg ggaaggtgcc
     1921 tttggaggtc tggtgggagg actccttggc ggactggtcg ccggtttgtt cggcatagtc
     1981 ccgacgctct acggagctct cattggtgtt ggaggaagtg tcgttgccca agtaggcgat
     2041 cttttggaat cggccatgaa gagggagtgc ggcatcaagg atagtggttt catccttcca
     2101 ggtcacggcg gggttttgga tcggtgtgac agcatgattg tccttgcacc gtttctctat
     2161 ttggcaatca gctggattct gaggtgaact tttaatgaag actgtggcaa ttattggcag
     2221 caccggatca ataggcactc aagcattaga cgtactacgg gatctagacc atgagtttca
     2281 ggttgtagcg ttggctgcaa acaccagcgt agacaagctc tatgcacaga ttggagagtt
     2341 tcagccgcgt cttgtggctg tgcaggaccc aaaggccagg gaagaactgg aagcccgctt
     2401 aacgggctct attgcgcagg ttgtaggtgg ggacaagccc ctggaaaccc tagtcttgga
     2461 agcggatgcc gatatcacca ttgtcagcgt tgttggagca gctggtctct tgccagccct
     2521 tgcagctgca aggcttggta aaagacttgc actggcaaac aaggagtctt tggttctagg
     2581 tggagagctc cttttggctg aatgcagcca gcacggaacc gaactcatac ctattgacag
     2641 cgaacactcg gctattttcc agtgcttact tggtcaagat atagatgcag tggggaaaat
     2701 cgtccttact gcatcagggg gaccattctt gcatgtgcca gctgaaaaga tgcgagttgc
     2761 agcgctagaa gatgctctcg ctcatccaac gtgggatatg ggtggcaaaa tcaccattga
     2821 ttcagctaca atgatgaaca aagggcttga ggtcatcgaa gcccactacc tgtttggcat
     2881 agactacgat agaatagaag tgttggttca tccgcaatca ataatccatt cttatgttga
     2941 atataatgat ggtgcgatca tcgcccagct gggcattgcg gacatgcggg tgccaattca
     3001 gctggcgctg acctaccccc agagaaagcc caggtccgaa aggctgaatt tggtaggaaa
     3061 gaccttggag tttttcgcgc cggatctgga aaagttcggc tgcttaaggc ttgcgtatga
     3121 ggctggaaga gcaggtgggg ataggccagt tgtgttgaat gccgccaacg aagtggctgt
     3181 ggaacacttc ctcaaacgga agatcacctt tggcggcatc tacgaggttg ttctagagac
     3241 ccttgagcgt tttcccacaa gatcagcttc gaccctgtcc gaactgctcg aagttgataa
     3301 gctggtaaga gagtttgccg tcggtcttat agaaaccggg aggtggagaa cataatcgtt
     3361 caactgataa cgattgtttt ggtatttgcg gtccttgtga tcgttcacga actaggtcac
     3421 tttcttgtgg caaaggctct gggaatctgg gtttacgagt tcagtatcgg ttttggacct
     3481 gtactgttca aaaagaagac taaagagact cagtactcca tccgagcatt tccatttgga
     3541 gggtttgtca gcgtcgctgg catggatgga gcagcagacg cagaaagagg agacgttccg
     3601 cctgaaaggc tttacaccaa taagcccgct tggggcaaag tggccacaat tgccgctgga
     3661 ccgttgatga acatcgtgtt tgccatggtg atttcagtgc tgatcatcgg catgagcggc
     3721 aggcaagttc cggtggtagg acagtcggta cctggaagcc ctgcagaacg ggcgggcatt
     3781 cagtccggag acgagatttg ggccgttgac aacatgaacc tttacaacca gggccagttt
     3841 ctgcagctga ttgcctcaag tcaggggcag agtctgcagc tcaaggtaaa aagagatgac
     3901 accttcctga cttttgaagt tagtcccgag ttcaacgagg agctcggccg atccatcatc
     3961 ggcgtagacg gtttgacgta caagcagata ccgctgaacg ctttgaacct gttggtttcc
     4021 ggctgggaca acaccaagat catgatagtg accctattta ggggactgaa agccatgatc
     4081 accggaaaag tggaagcgga cgttgccagc cccatcggca tggttcagat catcggcgaa
     4141 acagcatcgt acatggaagt taacttcttg ctcggtctgc tcaacgtaat gtatcttgcg
     4201 gtgttcctct cggtgaactt tgccatagtc aaccttttgc cggtcccaat cctggacgga
     4261 ggttggatcc ttctgatcat catcgagttg atccgcagga aacctctgac cgagagacaa
     4321 aaagctatcg ctcagatgac cggtctggtc tttattgctg ggttgttttt gtttgcaacc
     4381 tacagcgatt tctcccggtt gggctggttg agcttcttgg aaagatagtg ataggatgaa
     4441 agttcgcgaa gttaagattg gacctctcca gcttgggggc gatgcccccg tgctcattca
     4501 gtccatgacc aatacgaaga cggaagacgt caaagccacc ttagagcaga tcaacgctct
     4561 gtgcgaggtt ggctgcgact tcgtccgcgt tgcagtccct agtaaagaca ccatcaaggc
     4621 gtttaaagag atcgtgaagt ctgcaccttg tccgattgtt gccgatattc actttgactg
     4681 gcgtttggcg gtagaagcca tcgaagccgg tgcaagcaaa gtccgggtaa accccggcaa
     4741 tctcggaggt ctggacaaac tccgggaagt tgccagagct gcaagtggca gaagtgctat
     4801 acgcgtagga gttaactctg gcagcatcgg cagggattac ctagcgaaga tacagtccgg
     4861 cgagatcacc aaagctgaag caatgaccag atccgccaag gattacgtgg atttcctcta
     4921 cgacagcggt ttttacgacg tggtggtatc gctaaaggca tccgatattc ccacgactct
     4981 tagcgtcaac aggacctttc gccagatctg cgacagccct ctgcatcttg gcataaccga
     5041 agccggtaca gcgttttcgg ggaccattaa atctgcagtt ggattaggag tactgcttct
     5101 tgaagggatc ggggagacta tcagagtgtc tctaactgca cctcccacag aggaggttaa
     5161 gttggccaaa gagattctca aagccaccgg acgcagaaag atgggaccaa ccatcatctc
     5221 ctgcccgaca tgcggcagaa cagagatcga tctttacaca cttgcaaccc aggtagaaaa
     5281 gctcactgaa tccataccag aggacatcac cattgcagtg atggggtgcg ttgttaacgg
     5341 acctggggag gcaagagaag cagacatagg tgttgccgga ggcagaggtg caggtgtaat
     5401 ctttgtgggt ggagaaatag tagagaaggt taaggaagaa gatctttttg aacggtttac
     5461 cgtgcattta gagcgccttc tacaggaaaa gaaacagaaa gacgcctcga agtaaacagt
     5521 ttgaggcagt ccgggaaaag aacaagaata gaggtgtgga ggttgaagat agcagctatc
     5581 gttccagcct acaacgagga ggacaacatc agtaaagtcc tcgtacctct tgggtttgca
     5641 aaagaacagg gctacatcca ccaggttttg gtcgtctgtg atggctgtga ggatcgcaca
     5701 gcagacgttg ccaaaagctg cggagcagaa gtcctggaac tgaatcccaa cagaggtaaa
     5761 ggcggagcaa tgctcgaggg agcaatgttt accgatgcgg acatgctcgt attcgtagac
     5821 gctgatctca tcggactaac cccggaacac attagcagtc taacaaaacc tcttcttgag
     5881 ggtagagcga aaatgagcat gggaattttc ggacacggaa gattttccac agacgcagcg
     5941 cagaaagtag cgccgtttct ttcaggtcaa cgggcgattt tccgtgaaga ttttttgaaa
     6001 atacccaacc ttgccacatc ccgctacgga gtagaaatcg agattacgaa gtacagcaaa
     6061 aagcacagat ggaaagtaca acgcgtagtg tgggatcaaa tgagtcaggt tatgaaagaa
     6121 gaaaaattgg ggtttttcgg cggcttaaag gtccggctga gaatgtactg ggagatcatt
     6181 aaagcttcta tgtaaaaccg aaaaacgctg tacggacgag agcactcgtg gcgcaggtca
     6241 tgagtgttct taattgtgaa agagtcgtcg tcttgcggag ggaggtgcac acatggcaac
     6301 ctacattatc gaaccaaacg aagaaaaatg ccgaaagctc ctaccggatc tccctgtccg
     6361 gatagtgcgc gtagtagtag atgaggaacg ggggcgcttg gaggtcgacg ttagaagcca
     6421 tacagaactg gacttcaaga ccaagcaaaa gatcagagac gccatcaagt cccacattcc
     6481 tgaagcaaga accattaacg tctgctgctg gcttgaggac tcagactata cgggagataa
     6541 gcctaa
//
LOCUS       DAOSDA010000031         6505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_7575_length_6505_cov_3.860155, whole genome shotgun sequence.
ACCESSION   DAOSDA010000031 DAOSDA010000000
VERSION     DAOSDA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7575_length_6505_cov_3.860155"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /gene="tig"
                     /locus_tag="PLW82_01330"
     CDS             <1..885
                     /gene="tig"
                     /locus_tag="PLW82_01330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOJ45948.1"
                     /translation="KCYSQLVAVDVRDTVEEGDHIIFDFKGFVDGEPFEGGAAENYAL
                     KIGSGTFIPGFEEQMVGKKLGEKSEITVTFPEDYHMEALQGKETVFEVKVNEIKKEVL
                     PELDDEFAKDVSDKETLAEYREQIKKDLDENFERISRNKLEDEILAKLLEATSFDPPP
                     SMVDMQAQQLLEEMKTNLAYQGISWEFYLQRTEQDEEALKENFKPRALHQVKNYFLLK
                     EIALKEGIEVSDEELDARIEELAEQFQMTKEQLREYYEKSGQLGSLRSEIETEKTLQF
                     LVDNAIIEEQVVDPKELS"
     gene            923..2170
                     /gene="clpX"
                     /locus_tag="PLW82_01335"
     CDS             923..2170
                     /gene="clpX"
                     /locus_tag="PLW82_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOJ45949.1"
                     /translation="MPKYSDDRNQLKCSFCGKTQEHVKKLIAGPGVYICTDCIELCNE
                     IIEEEFADGLDETLQSIPKPKEIKEILDEYVIGQEEAKRILSVAVYNHYKRITCSSKS
                     DDVELQKSNILLLGPTGSGKTLLAQTLAKILNVPFAIADATTLTEAGYVGEDVENILL
                     RLIQAADYDVERAERGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFIQIDTTNILFICGGAFDGLEKIIERRIGKRGLGFGGTIKATGEK
                     SIGEIFTQVMPEDLLKYGLIPEFVGRVPILCSLDALDEEALVRILREPKNALVKQYQK
                     LLEMDNVKLTFDDEALTAIARKAIERKTGARGLRAILEELMLDLMFEIPSDPDIKSVR
                     ITKEVVNEGVRPLVSKSQKEETA"
     gene            2284..3951
                     /gene="lonB"
                     /locus_tag="PLW82_01340"
     CDS             2284..3951
                     /gene="lonB"
                     /locus_tag="PLW82_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HOJ45950.1"
                     /translation="MNAFVQFLVILSMIFSIIVGIYFWNLLKAQQGSKTAVEKESQKE
                     MDKLLRLRSISLSEPLAEKTRPANFRELVGQEDGLRALRAALFGPNPQHVIIYGPPGV
                     GKTAAARVVLEEAKHSKGTPFLPDAAFVEIDATTARFDDRGIADPLMGSVHDPIYQGA
                     GPLGVAGVPQPKPGAVTKAHGGILFLDEIGELHPIQMNRLLKVLEDRRVFFESAYYSP
                     DNKNIPEHIHDIFQNGLPADFRLVGATTRSPEEIPPALRSRCTEIFFRALTEDEIARI
                     AEGALKKIGAKYDPEVIDVITQHAYSGRDAVNMVQIAEGLAFSEGKDRIRKEDVEWVV
                     TSGHYVPRPLVKASSERIVGAVNGLAVYGPHLGSVMDIEAKAFYLANGTGKLVVTGIV
                     EEEEIGQGYGQKLRRKSLVRSSLENVMTVLGYIIGEDLSSYHIHVNFPGGIPVDGPSA
                     GLALAALLYSAIKNEPVPNDYAFTGEVSVRGMVRPVGGVPAKIDAAVRAGVKKVFIPK
                     ANYKDYFEGLPLKVVPVAKVEEMLEEVFRSRALLFASAGSLVSKVNG"
     gene            3995..6367
                     /gene="lon"
                     /locus_tag="PLW82_01345"
     CDS             3995..6367
                     /gene="lon"
                     /locus_tag="PLW82_01345"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOJ45951.1"
                     /translation="MHSRNLEDSQKPITSLREEWPLMPLRGMLVYPGMVTPLEVGRER
                     SINALEQAMVSDRMMILAAQKEARVKEPEIDDIYEIGTLVEIKQLLKLPEGHIRILVE
                     GISRVRILSLVQSDPYYTANIEILTVDEKLDRETEALMRSASSLFEKYVKLSKKLPLE
                     VVASLDEISEPGKLADNIASQLLIRVEDKQQILETFDVKERLELLNVLLSKELEILEL
                     EKNISTRVRRQMDKSQRDYYLREQLKAIRQELGEDEDKYEELEEYRQKIEKLGLSPDT
                     KEKALKEVSRLEKMPPGTAEAVVVRNYLDWIIALPWKTETKDNIDIIKAESILDADHY
                     GLEKVKERILEFLAVKKLTKSMKGPILCLVGPPGVGKTSLAKSVARALNRKFVRFSLG
                     GVRDEAEIRGHRRTYVGALPGKIIQSMRDAKSKNPVILLDEVDKMSSDFRGDPASALL
                     EVLDPEQNNAFQDHFLEVPFDLSRVMFITTANNAMDIPQPLLDRMEVIEVPGYTEVEK
                     MQIALRHLMPKQLEAHGLKEGDVEISQTTVLAIIREYTKEAGVRNLERTLASLYRKAA
                     KEIVAGREKPIRITKNNLEKYLGVPSYTFGIAETEDKVGVATGLVWTPVGGDIVSIEV
                     SVVPGKGGLTLTGKLGDVMKESAQAALSYTRYRAEDLGIDPDFHDHKDIHIHVPEGAV
                     PKEGPSAGITMATALVSALSCKPVRWDVAMTGEITLRGRVLPIGGLKEKLLGAQRAGI
                     KTVLIPKANERDLKEVPKEILKGLKVIPVGHMDEVLKHALRVKEEITFND"
BASE COUNT         1821 a         1521 c         1782 g         1381 t
ORIGIN      
        1 aagtgctaca gtcagttggt tgcagtagac gttcgcgata ctgttgaaga aggcgatcac
       61 attattttcg acttcaaagg ttttgtagac ggcgagccgt ttgaaggcgg tgcagcagag
      121 aactacgctt taaagatcgg aagtggtaca ttcattcccg gtttcgaaga gcagatggtg
      181 gggaaaaagc taggtgagaa gtccgaaatc actgtgacct tccctgaaga ctaccatatg
      241 gaagccctcc agggcaaaga aaccgtattt gaagtaaagg ttaacgaaat caagaaagag
      301 gttcttccag agctagacga cgagtttgcc aaagacgtca gcgacaaaga aactcttgca
      361 gaatacagag agcagattaa gaaagatttg gacgagaatt tcgaacggat ctctcggaat
      421 aagctagaag acgagattct ggcgaaactg ctcgaagcca caagtttcga tccacctcca
      481 tcaatggtag atatgcaagc ccaacagctc ctcgaggaga tgaagaccaa tcttgcatat
      541 caggggatca gttgggaatt ctatttacag cgtaccgaac aggacgaaga ggctctcaag
      601 gagaatttca aacctagagc cctccatcag gtgaagaact acttccttct gaaggaaatc
      661 gctctgaaag aaggtatcga agtatccgac gaggaactcg atgccaggat cgaagaactg
      721 gctgaacagt tccagatgac caaagagcag ctccgcgagt actatgaaaa gagcggtcag
      781 ctcggcagcc tgcgctctga gattgagact gaaaagactc tccagttttt agtggataat
      841 gctataatag aagaacaggt ggtagatcct aaagagctat catagattgt caccaaaact
      901 ggagacagtg gggtgatcac aaatgcccaa gtacagtgac gatagaaatc agctgaagtg
      961 ttccttctgc ggcaagacgc aggaacacgt taaaaagctc attgccggtc caggtgtgta
     1021 tatctgtacc gactgtattg agctgtgcaa tgagattata gaagaagagt ttgccgacgg
     1081 actggacgag actctgcagt ccatccccaa acccaaagaa atcaaggaaa ttcttgatga
     1141 gtacgtcatc ggtcaggaag aggccaagcg gattctttct gtagcggttt acaaccacta
     1201 caagaggatt acctgcagct cgaagagcga cgatgtggaa ctccaaaaga gcaacattct
     1261 cctgctcggg cctaccggtt ccggtaagac gctgctggcg cagactctgg caaagatcct
     1321 caacgtgccc tttgccatcg ctgacgcaac gacgctcacc gaagcgggat acgtcggtga
     1381 agacgtggaa aacatcctgc ttaggctgat ccaggcagca gactacgatg tggaacgggc
     1441 cgagcgcggc attgtctata tcgatgagat cgacaagatc tcgcgcaagt cagacaatcc
     1501 ttccataacc agagacgtat cgggagaggg agtccaacag gcactcctga agattttgga
     1561 aggtactgta gccaatgtgc ctcctcaagg aggtagaaag cacccgcacc aggagttcat
     1621 ccagatcgat accaccaaca tcctgttcat ctgcggggga gcatttgacg gcttggagaa
     1681 gatcatcgag cgccgcattg gcaagcgcgg tctcgggttt ggcggcacca tcaaagccac
     1741 aggagagaaa tctatcggcg agatcttcac gcaggttatg cctgaagatc tcctgaaata
     1801 cggtctgatt ccggagttcg tgggcagagt gccaattctc tgctcgctgg atgctcttga
     1861 tgaagaggcg ttggtcagaa ttttgaggga gcctaagaac gcccttgtca agcagtacca
     1921 gaagctcctg gaaatggata acgtgaagct gaccttcgac gacgaagccc ttaccgctat
     1981 agcccgaaag gctattgaaa gaaagaccgg ggctcgcggt ctccgcgcca ttttggaaga
     2041 gctcatgctg gatttgatgt ttgagattcc atcggatcct gacatcaagt cggtgcggat
     2101 aaccaaagaa gtggtgaacg aaggtgtaag gccgctggtt tccaagagtc aaaaagaaga
     2161 gactgcataa aaagcaaatc tgagaactcc cgatttaggg agttcttttt ctttgcgaag
     2221 gaaaggttaa aggcatgagg catggatata ctaacaacgc agtaagtttt aggaggtctg
     2281 ccgatgaatg cctttgtgca gtttctagtc atactgagta tgattttttc gattattgtc
     2341 ggaatctact tctggaatct cttaaaagcc cagcagggaa gcaagaccgc agtggaaaaa
     2401 gagagccaga aagagatgga caagctgctg cggctcagat ccatatcgct ctcggaacct
     2461 ctagcagaaa aaacgaggcc tgccaacttc agagagctgg ttggtcagga agacggactc
     2521 agagcccttc gcgctgctct cttcggacca aatccccagc acgttatcat ctacggtcct
     2581 ccgggagttg gcaaaactgc agcagcaaga gttgttctgg aagaagccaa acactcaaaa
     2641 ggcacgccgt ttttgccgga tgcagccttt gttgagatag atgcgactac agctagattc
     2701 gacgatcggg gcatcgccga tccccttatg ggttcggtgc acgatcccat ctatcaggga
     2761 gccggccccc tcggggtggc aggtgtcccc cagccaaagc cgggcgctgt gaccaaagcc
     2821 cacggcggca tcttgttttt agatgagata ggcgagctgc atcccattca gatgaacaga
     2881 ctcctgaaag ttttagaaga ccggagggtg ttctttgaaa gcgcttacta ctctccagat
     2941 aacaagaaca tccccgaaca cattcacgat atcttccaaa acgggcttcc tgcagatttc
     3001 cgcctggtgg gcgcaaccac cagaagtcct gaggagatac cgccggctct ccgttcaagg
     3061 tgcacggaga tcttcttcag agctctcacc gaagacgaga tcgcaagaat cgccgaaggc
     3121 gccctgaaga agatcggcgc aaagtacgat cccgaagtga tcgatgtcat tacgcagcac
     3181 gcctactccg ggcgagatgc ggtgaacatg gtgcagatcg ccgaaggcct tgccttcagc
     3241 gagggtaaag acagaatcag gaaagaagac gtggagtggg tagtcacatc aggccactac
     3301 gtgccaaggc cgctggtaaa ggcgtcttca gaacgaatcg tcggcgctgt aaacggtctg
     3361 gcagtgtacg ggccgcacct gggctctgtg atggacatcg aagccaaggc cttctacctg
     3421 gcaaacggca caggtaagtt ggttgtaacc ggaatcgtcg aagaagaaga gatcggacag
     3481 ggttacgggc agaagctcag gcgcaagagc cttgtgcgct cctcgctgga aaacgtcatg
     3541 accgttttgg ggtatattat cggggaagac ctatcaagtt accacattca cgttaacttc
     3601 ccagggggaa ttcctgtaga cggtccgtcg gcagggctag cgcttgcagc tctactgtac
     3661 tctgccatca agaacgaacc ggttccaaac gactatgcct tcaccggcga ggtcagcgtg
     3721 agaggcatgg tccgtcctgt gggaggggtg cctgccaaga tcgatgcagc ggtcagggca
     3781 ggtgtgaaga aggtcttcat tcccaaagcc aactacaaag actacttcga aggccttccg
     3841 ctgaaggtag ttcctgttgc gaaagtagaa gagatgctcg aagaagtatt tcgcagccga
     3901 gcgctgcttt tcgcatctgc aggaagtttg gtcagcaaag taaacgggta atgtagagtt
     3961 aagaaatgga ggtggctgtt atatggaaaa agatatgcat tccagaaatc tcgaagatag
     4021 ccaaaagcct attacctcct taagggaaga atggccgtta atgccacttc gaggaatgct
     4081 ggtttaccct ggcatggtga ctccacttga agtgggtcgc gaaagatcca tcaatgcttt
     4141 agagcaagcg atggtttctg acaggatgat gatacttgca gctcaaaaag aagccagggt
     4201 gaaagagcct gagatagatg acatttacga aatagggacc ttggtagaga ttaagcagct
     4261 tttgaagctt cccgaaggtc acattcggat tttggtagag ggtatcagta gagtgcgaat
     4321 tctcagtctc gttcagagcg atccgtacta cactgccaac atcgaaatcc tgaccgttga
     4381 cgagaagctg gacagggaga cagaagccct catgcggagt gcttcgtcgc tctttgaaaa
     4441 gtacgtgaag ctttctaaga aacttcctct agaagtagtt gcgtccctgg atgagatctc
     4501 cgaaccggga aagcttgccg acaacatcgc atcgcagctt ttaatcaggg tagaggataa
     4561 gcagcagatc ttggagacct ttgacgtcaa ggagcgccta gaacttctta acgttcttct
     4621 ctccaaagag ctggagattc tcgaactgga aaagaacatc tccacccgcg tacgtcggca
     4681 aatggacaag agccaaagag actactacct cagagagcag ctcaaagcca ttaggcagga
     4741 gctcggcgaa gacgaagaca agtacgaaga acttgaggag tacaggcaga agatcgagaa
     4801 gttaggccta agtccggata ccaaggaaaa ggccttgaag gaagtctcca ggctggagaa
     4861 gatgccgcct ggaaccgccg aagctgtagt tgtgcgcaac tacctggatt ggataattgc
     4921 tttgccttgg aaaaccgaga ccaaagataa tatagatatt atcaaagcag agagtatcct
     4981 tgatgcagac cattacggtc tggaaaaggt taaggaaaga atcctggagt ttttggcggt
     5041 gaaaaaactg accaagagca tgaagggtcc aatcctgtgc ctagtggggc cacctggagt
     5101 aggtaaaact tcgctggcaa agtcggtggc aagagcccta aaccgcaagt ttgtgcgctt
     5161 ttcgttaggt ggagttcgcg atgaggccga gattagaggc cacaggcgga cctatgtagg
     5221 agctctaccc ggcaagatca tccagtccat gcgcgatgca aagtctaaga atcctgtgat
     5281 tcttctggac gaagtagaca agatgtcctc ggactttaga ggagatccgg cttcagccct
     5341 ccttgaggtc ctagacccag aacagaacaa cgctttccag gatcatttcc tggaggtgcc
     5401 tttcgatctt tcgcgagtaa tgttcatcac tacggcgaac aacgccatgg atataccgca
     5461 accacttcta gaccgtatgg aagttatcga agttccgggc tacactgagg tggagaagat
     5521 gcagatcgct ctccgccacc tgatgcccaa gcaactagag gcccacggac taaaagaagg
     5581 cgacgtggag atctcgcaaa cgacggtact tgccatcatt cgcgagtaca ccaaagaggc
     5641 cggcgtgagg aacttagaga ggactctagc tagtctctac cgcaaggctg caaaagaaat
     5701 cgttgcaggt agagaaaaac ccatcaggat cacgaaaaac aacttggaga agtacctggg
     5761 cgtaccttcc tacactttcg gcatagccga gactgaagac aaagtgggag ttgccacagg
     5821 ccttgtgtgg actcctgtgg gaggagacat cgtctctatt gaggttagcg tagtccctgg
     5881 aaagggcggt ttaaccctca ccggtaagct aggcgacgtc atgaaagagt cggctcaagc
     5941 ggctctcagc tacacccgct acagagcaga agatctgggt atcgaccccg acttccacga
     6001 ccacaaggac atccacatcc acgtacctga aggtgcagtt cccaaagaag gtccttccgc
     6061 gggcatcacc atggcgacgg ctttggtatc agccctcagc tgcaagcccg tacgctggga
     6121 tgttgctatg accggagaga ttaccctcag aggcagggtc ctgcctattg gaggactcaa
     6181 ggagaaactt ctcggagcac agagggccgg tatcaagacg gtcttgattc ccaaagccaa
     6241 tgagcgggat ctcaaagaag tgcccaaaga gatccttaag ggacttaagg tgatccctgt
     6301 agggcacatg gatgaggttc tcaaacacgc cttaagggtt aaagaggaaa taacgttcaa
     6361 cgactaagtt ttctgcgctc cccgtagata gcagcggaca ctggtccgct gcctatcttg
     6421 gggagttttt ttgtgctttg gaggggctta gtacaggtag agtctggtat aatcgaaggg
     6481 aattgatgaa tgcggaggaa ctgac
//
LOCUS       DAOSDA010000032         6503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_7583_length_6503_cov_3.407568, whole genome shotgun sequence.
ACCESSION   DAOSDA010000032 DAOSDA010000000
VERSION     DAOSDA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7583_length_6503_cov_3.407568"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /gene="pheS"
                     /locus_tag="PLW82_01350"
     CDS             <1..644
                     /gene="pheS"
                     /locus_tag="PLW82_01350"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOJ45952.1"
                     /translation="YTIADGREVEDDFHNFEVLNIPKDHPSRDMQDTFWISSDTLIRT
                     HTSSVQSRVMEKQDPPVRIIAPGRVYRCDDDVTHSPMFHQVEGLVVDKGITFGDLKWT
                     LTVFIKALFGQERQVRFRPSYFPFTEPSCEVDVSCFVCNGKGCRLCKNTGWIEILGAG
                     MVHPNVLKIAGYHTKVYTGFAFGLGIERIAMLRYGIDDIRLFYESDMRFLSQF"
     gene            657..3020
                     /gene="pheT"
                     /locus_tag="PLW82_01355"
     CDS             657..3020
                     /gene="pheT"
                     /locus_tag="PLW82_01355"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOJ45953.1"
                     /translation="MRVPIEWLKDYVDTDKDAETLADLLTKTGSEAEGIEVLGNRVEN
                     LVVGLIERIEKHPNADRLVVCQVNVGSETVQIVTGAPNVEEGMLVPVVLPGAVLADGT
                     KIKAAKLRGVESFGMLCSKEELGLEEKSEGILVLPQDEGIQPGQDVNELLKLGSPIVS
                     FGITPNRPDCLSMVGMAREVGIIEGKPLRLPRPEVSEVPSKAADVVKVTVKARDLCPR
                     YACRVIEDVKLGPSPLWMQQRLIAAGMRPINNIVDITNYVMLELGQPLHAFDADKIAE
                     GEIIVREAEENEVLKTLDGVERKLEKGMLLIADPTQPLALAGIMGGESSEITESTKRV
                     LLESANFNRGSIRRTSRKLGLRSEASSRFEKGLDPELVFLAADRAAELLEKYAGGKVY
                     SGTVVDGDYEAKETVVETTASFINRLLGLELEKSQIVEILTKVGLEVASNGDALTVKV
                     PSFRQDIEQEVDLAEEVARIYGLDNIPDSIPKGEIASSGLTQLQKVERILARELASIG
                     YSELITYSFLSEKRLEGLPGEPVRIANPLSEEQAVLRTSLLPSLLKAVETNYNRLQKD
                     VAFFEIAKIYLKSEGQALPEEKLMLGAALAGKKQGYFDAAPGDVDFYDLKGALDYLGS
                     RLRLTFDYAPSQRPRMHPGRCADVSLDGRVIGYIGQIHPDETEEPVYFLEIEVEPLTK
                     GLSEVVVYKGLTRFPAVFRDIALVGPMAVPAKELTEAIINAGGRHLVSVELFDLYTGP
                     QVGEGKKSLAYALMFQSEERTLTDSEIDEALKNIQAKVAEKDFIFRA"
     gene            3094..3369
                     /locus_tag="PLW82_01360"
     CDS             3094..3369
                     /locus_tag="PLW82_01360"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOJ45954.1"
                     /translation="MEKELKRVKIHLFGKDYIVRTDLPAERLEGMAVELDNKLEALRS
                     RYPNLTRTDVALLTALSLMEDLEKLREEKKELWELLGEATSKTPETT"
     gene            3403..3972
                     /locus_tag="PLW82_01365"
     CDS             3403..3972
                     /locus_tag="PLW82_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOJ45955.1"
                     /translation="MKNWLDWLFIGLGILAIWKGYVRGFIREVLEIIGIVVAFIAAIH
                     YAPQVAVFFTESWNMPPNLSAVLSYAVVLTGVAILVQLVIYFTAPFISSGVLGILDSS
                     VGGFFSLLKLALIVIIVLNLVVLGPLQFLKTPVMDSQVANYLLSITPELYDFMIDKFP
                     ENWAEQLETYKEQFMAPRQEIEVDPTRRI"
     gene            3975..5621
                     /locus_tag="PLW82_01370"
     CDS             3975..5621
                     /locus_tag="PLW82_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ45956.1"
                     /translation="MFEPNPHVAGALEELAFLLEITGGNPFKIRAYRQAARTLKSFPE
                     PLEDVRQLDGIKGIGAGIKGIVEEVLSGALPEELRELRDQVPAGIFDIARLPGLGPKS
                     LKTLMDSGITDLETLEEKAKDKSLAALPGFGQKTVERILESLEELKNHMDLHLGYDAL
                     LFARRLTDKICQFPGNKIIETQITGEVRRFAPQVTTVEIAVKTVPDFSEEDLAEFLLL
                     ESTGENGIYRHPEGYTVKLYFATDKDFGTVLLRTTGPEEFTSAFGELLDAPDEKTLFA
                     NKGFFFVPPELRHTAFASDYLRGSPPRLVELGDIKGDLHVHSNYSDGVATILEMAKKA
                     QSLGYQYIGISDHSKSLVIANGLDGERLLEQGREIDALCSAGDLDIWILKATEVDILK
                     DGSLDLDDDVLSKLDFVTASVHSSFRMTKTEMTERIIRAMENPFVRSIGHITGRLLGR
                     RPGYDIDWDRIIKEAKKTSTALELNASPERLDVPEEFLREVTSNGVKIVINTDAHSPE
                     GLMDMVWGVMTARRGLVEPHHVLNTYSAADFKSWLREGHK"
     gene            5766..6482
                     /locus_tag="PLW82_01375"
     CDS             5766..6482
                     /locus_tag="PLW82_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ45957.1"
                     /translation="MEEKLLVEMEDVTAGYPGHIAFRDVSIKVSSKEHVAIVGPNGGG
                     KSTMLKVMMGLLPPKAGVVKVCGQNPYKNRCVKKRVSYLPQQSTINSHFPALIEDIVL
                     LGLFGELGLVGRPTKEQNLRIEEALERVGLTNMIGEPFEHLSGGQKQRVLIARAFVGR
                     PSLILLDEPTTGLDVASQEAIIEIIQDLTENEGITIIAVTHDQIFLQKWAKRIIRIDG
                     RLVFDGPIEDYQGVTLNGYS"
BASE COUNT         1750 a         1506 c         1757 g         1490 t
ORIGIN      
        1 gttacaccat cgccgatggc cgcgaagttg aggacgattt ccacaacttc gaagtcctca
       61 acattccgaa ggatcaccca tctagggaca tgcaggacac gttctggatc tcttcggaca
      121 ctctgattag aactcatacc tcgagcgtgc agtcgcgagt aatggagaaa caggatccgc
      181 ctgttaggat cattgctcct ggaagagtgt accgctgcga cgatgacgtt acccactcgc
      241 ccatgttcca ccaagttgaa ggccttgttg tggacaaggg gatcaccttc ggtgatctca
      301 agtggactct caccgttttc atcaaagccc tgtttggaca ggaacgtcag gtaagattcc
      361 gtcccagcta cttcccgttc accgagccca gctgtgaggt ggacgtcagc tgctttgtct
      421 gcaacggtaa gggttgcaga ctttgcaaga atacaggctg gattgaaatt ctaggtgctg
      481 gcatggtaca ccccaatgtg ctgaagatcg caggctacca caccaaagtc tacaccggat
      541 tcgccttcgg tttgggtata gagagaatcg ccatgctccg ctacggcatc gatgatatca
      601 ggctattcta tgaaagcgat atgcgctttc tcagccagtt ttaggaggga gagaaaatgc
      661 gagtaccgat agaatggtta aaagactatg ttgatacaga taaagatgca gaaacgttgg
      721 ccgatcttct aactaaaacc ggttctgaag ctgaaggtat agaggttttg ggcaatagag
      781 tcgaaaacct ggttgtgggg ctgatcgaac ggatcgaaaa gcacccaaat gccgacaggt
      841 tggttgtgtg ccaggtgaac gtcggcagtg agaccgttca gatcgttacc ggagctccta
      901 acgtcgaaga aggcatgcta gttccggtgg tcctcccagg agcagtgctt gccgatggaa
      961 ccaagatcaa agcggctaaa cttcgcggag tagagtcctt cggtatgctg tgctccaaag
     1021 aagaactggg ccttgaggag aagtccgaag ggatcctggt tttgcctcaa gacgaaggaa
     1081 tccagccggg acaggatgta aatgagctgt taaaactcgg ttcaccaatc gtttctttcg
     1141 gcatcactcc caacagaccc gattgcttga gcatggttgg tatggcgcga gaggttggca
     1201 ttattgaagg taaaccactg cgcttgccac ggcctgaggt ttcggaagtt ccaagcaaag
     1261 ccgcagatgt ggttaaggtc acagtcaagg ccagagatct ctgtccgagg tacgcctgtc
     1321 gcgtcattga agatgttaag ttgggaccgt ctcccctgtg gatgcagcag aggctgattg
     1381 ctgcagggat gaggcctatc aacaacatcg ttgacattac caactacgtt atgcttgagc
     1441 taggtcaacc actgcacgcc ttcgacgcag acaagatcgc agaaggcgag atcatcgtta
     1501 gagaagctga agagaacgaa gtgctgaaga ccctagacgg tgtagagcgc aagctggaaa
     1561 aaggtatgct cctaatcgcc gatcccactc aacctcttgc ccttgcagga atcatgggag
     1621 gagagtccag tgaaatcacc gaaagtacca aacgcgtgct cttggagtct gcgaacttca
     1681 acagaggttc tattagaagg acctccagga agctcggtct tagaagcgaa gcatcttcca
     1741 ggttcgaaaa gggactcgat cccgagctgg tcttcttagc tgcagacaga gcggcagaac
     1801 tcctagagaa gtacgcaggc ggcaaggttt actcgggcac cgtcgtcgat ggcgactacg
     1861 aagcaaaaga aacggttgtt gaaacaaccg catccttcat taatagatta ttgggccttg
     1921 aattggaaaa aagccaaatt gtcgagattc tcaccaaggt aggcctggag gtggcctcca
     1981 acggagacgc tttaactgtc aaagttccaa gcttcaggca ggatattgaa caggaagtag
     2041 atctggcaga agaggtagca aggatctacg gcctggacaa tattccagac agcattccca
     2101 agggagagat cgcatccagc ggccttacgc agctgcagaa ggtcgagcgg atcctcgcca
     2161 gggaactagc atctatcggc tattcggagc tgatcaccta cagcttcctt tccgagaaaa
     2221 gactggaagg tcttcccggt gagccggtaa ggattgccaa ccccttaagc gaagagcagg
     2281 cggtcctcag aaccagcctt cttccgagcc ttctgaaagc cgtggaaacc aactacaaca
     2341 ggcttcagaa agacgtggca ttctttgaga tagcaaaaat ctatctcaaa tccgaaggtc
     2401 aagccctccc cgaagaaaag ctgatgctgg gagcagctct tgccggcaaa aaacagggct
     2461 acttcgatgc ggcccctgga gacgtagact tctatgacct gaagggcgcc ctggattatc
     2521 tcggcagccg cttgaggctt acctttgatt acgctccctc acaaaggccg cggatgcatc
     2581 ctggcagatg cgcagacgtc agcctagacg gcagagtcat tggctacatc ggtcagatcc
     2641 atcccgacga aaccgaggag ccggtctact tcctggagat cgaagtggag cctctgacga
     2701 aaggtctgtc ggaagtggtg gtctacaaag gccttacccg cttcccagca gtgttccgtg
     2761 acatagctct ggtaggtcca atggccgttc ctgctaaaga actgacagaa gctatcatca
     2821 acgccggagg caggcacctg gtttcggtgg agctgttcga cctctatacc ggtcctcagg
     2881 tgggagaagg caaaaagtct cttgcctacg cgctgatgtt ccaaagcgag gagaggactc
     2941 taaccgacag cgagatcgat gaagctctga agaacatcca agcgaaagta gcggaaaaag
     3001 acttcatctt tagagcttaa tgctataggg tcaaagacgt tccatgcagg aggttttact
     3061 cctgcatgga attattttta gggagggtgt aaaatggaga aagaactaaa acgagtaaag
     3121 atacacttgt ttggaaaaga ttatatagta agaacagact tgccagcaga aagacttgag
     3181 ggtatggcag tggaactaga taataaactg gaagcattaa gaagccgcta tccgaatctt
     3241 acgcgaaccg atgtagcgct ccttacggcg ctgtcgctca tggaagattt ggaaaagctt
     3301 agggaagaaa aaaaggagct ctgggagctc ttaggcgaag ccaccagcaa gacaccggaa
     3361 actacttgag tttaagggag ggtaagagct aagctcctaa gcatgaaaaa ttggttagac
     3421 tggttgttca ttgggcttgg aattctcgcc atatggaagg gttacgtacg gggatttatc
     3481 cgcgaggtcc tggagatcat cggtattgtg gtggccttta ttgccgcaat acactatgca
     3541 cctcaagtag ccgtgttttt caccgagtct tggaatatgc ctcctaatct atcggccgta
     3601 ctaagctatg ccgtagtatt gaccggagtt gctattttag ttcagctagt gatctacttc
     3661 actgcgccgt ttatcagcag tggagttctg gggatattgg actcatcggt gggtgggttc
     3721 ttctcgcttt tgaagctggc gctgattgtg attattgtgc tgaacctggt agtgctcgga
     3781 ccactacagt tcttgaaaac gccagtaatg gattcccagg tggcaaacta cctcctcagc
     3841 atcacgcctg aactgtacga ttttatgatc gataagttcc cggaaaactg ggcagaacag
     3901 ctggaaacgt ataaagaaca gttcatggcg ccacggcaag agatcgaagt ggatcccaca
     3961 aggaggatat agtattgttt gaacccaatc cgcacgtggc aggcgcactg gaagagctgg
     4021 cattccttct ggagatcaca gggggaaacc cgttcaagat cagggcttac aggcaggcag
     4081 cgcgaacctt aaagtccttt cccgagcctc tagaagatgt aaggcaactt gacggtatta
     4141 agggaattgg cgccggcatc aaggggatcg tagaggaggt cctcagcggc gcattgcctg
     4201 aagaattgcg agagctcagg gatcaagtgc ctgccggcat cttcgatata gcgaggcttc
     4261 cagggcttgg acccaaaagt ctcaagaccc taatggatag cggaattacc gatcttgaaa
     4321 cgctagaaga aaaagctaag gacaagtcgc ttgcagccct ccccggcttt ggccaaaaga
     4381 ccgtggagcg gattctagag agtcttgagg aactaaagaa ccacatggat ttgcatttag
     4441 gctacgatgc cctgcttttt gcacgccggc tgactgacaa aatttgccag ttcccgggaa
     4501 acaagatcat cgagacacag atcaccggag aggtcaggcg ctttgccccc caggtaacta
     4561 cagttgagat tgctgtaaaa acagttccgg actttagcga agaagaccta gccgagtttt
     4621 tgcttctgga aagtactggg gaaaacggaa tctacaggca tccggaagga tacaccgtga
     4681 agctgtattt tgccacagat aaagacttcg gtacggttct tctccggact acgggtcctg
     4741 aagagttcac ctcagctttc ggggagttgc ttgatgctcc cgacgagaaa accctttttg
     4801 caaacaaggg ctttttcttc gtccctccag agctgaggca tactgctttc gcttcggact
     4861 atcttcgggg ttcaccgcca aggctggtgg agctggggga tatcaaggga gacctgcatg
     4921 tccacagcaa ctactctgat ggagttgcca ccatcttgga gatggcaaaa aaagcccagt
     4981 ccctcggcta tcagtacatc gggatctccg accacagtaa gtccctggtt attgccaatg
     5041 gtcttgatgg agagaggctt cttgaacagg gaagagagat cgatgcactt tgcagtgcag
     5101 gggatctgga tatttggatt ctcaaagcta ctgaagtgga catccttaaa gacggcagcc
     5161 tggaccttga cgacgatgtc ctgtcaaaac ttgatttcgt aactgcatcg gttcactcta
     5221 gcttccgcat gaccaaaact gagatgaccg aaagaatcat tagagctatg gaaaacccgt
     5281 tcgtacggtc aattgggcat attacaggaa ggcttcttgg caggagaccg ggttacgata
     5341 tagattggga ccgcatcatc aaggaggcaa aaaaaacaag cacggctttg gagcttaacg
     5401 ccagtcccga aaggctcgat gtgcctgagg agttcctccg agaggtaact agtaacggtg
     5461 tgaaaatcgt gatcaacacc gacgctcact ctcccgaagg cctaatggac atggtttggg
     5521 gagtgatgac cgcaaggaga ggtttggtag aaccccacca cgttctgaat acttattccg
     5581 ctgcggattt caaaagttgg ctgagggaag gtcataaata gagccttaag cgcggtgctg
     5641 cgctttcaca ttgggtttca caaatctgtg gaaccccttt tcttactcag gagtaacggg
     5701 tatactttaa gtgaaaacta ttttcaatta gaacttcagg tctaatacta aagggatgat
     5761 ctactttgga agaaaaactg cttgtggaaa tggaagacgt taccgcaggt tatcctggtc
     5821 acatcgcgtt tcgagatgtg agcattaaag tgagttcgaa agagcatgta gccatagtcg
     5881 gcccaaacgg cggaggcaag agcaccatgc tgaaagtgat gatgggactg ctccccccga
     5941 aagccggtgt ggttaaggtc tgcggtcaga atccttacaa gaacagatgc gttaaaaaaa
     6001 gagtatcgta tctcccgcag cagagcacca tcaactcgca cttcccggct ttgatcgaag
     6061 atattgtgct gcttggactt ttcggagaat tgggattggt tggaaggcca accaaggagc
     6121 aaaaccttcg gattgaagaa gctttagagc gggtgggact gacaaacatg atcggcgagc
     6181 cgttcgagca tctgtctgga ggccagaagc agagagtgct tattgccagg gcttttgtgg
     6241 ggcgtccttc tttaatactc ttggacgaac ctactacagg tcttgacgta gcgtctcagg
     6301 aagccattat cgagattatt caagacctca ccgaaaatga gggcataacc attatagctg
     6361 taacgcacga tcagattttc ctgcaaaagt gggcaaaaag aatcataaga atcgatggca
     6421 ggcttgtgtt tgacggtcct atcgaagact accaaggagt gaccctgaat ggctatagct
     6481 gatgttctcg cttccatatt tga
//
LOCUS       DAOSDA010000033         6494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_7599_length_6494_cov_3.205156, whole genome shotgun sequence.
ACCESSION   DAOSDA010000033 DAOSDA010000000
VERSION     DAOSDA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7599_length_6494_cov_3.205156"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLW82_01380"
     CDS             <1..677
                     /locus_tag="PLW82_01380"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOJ45958.1"
                     /translation="LMQKEIEVMGKALANPERPFVAILGGAKVSDKIGVIENLLTKVD
                     TLLIGGGMAYTFLKAKGYEIGNSLLEEDKIDLAKELMEKAEKSNVKLLLPLDNVVADR
                     FAADAETQVVASTSIPAGWEGLDIGPKTRELFASEIKKAKTVVWNGPMGVFEMEPFAA
                     GTKAVAMALAECDGTTIVGGGDSAAAVEQLGFADKVTHVSTGGGASLEFLEGKELPGV
                     AALNDK"
     gene            836..1585
                     /gene="tpiA"
                     /locus_tag="PLW82_01385"
     CDS             836..1585
                     /gene="tpiA"
                     /locus_tag="PLW82_01385"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOJ45959.1"
                     /translation="MRTPIIAGNWKMNKTVDEALDLVRKLKESLKDVKDREIVVCPSF
                     VALYAVAKEIEGSNIKLGAQNCYYEDNGAYTGEVSPVMLKDIGVEYVVIGHSERRSYF
                     AESDDLVNKKAKALLKKGLKPIICVGESLEQREAGETENIVRKQVFRDLDGIEPYQLK
                     DVVIAYEPIWAIGTGKTATSEEANRVISLIRDTIKEMYSEKEASVLRIQYGGSVKPEN
                     ATELMTQSEIDGALVGGASLDAEAFEAIVKF"
     gene            1600..3123
                     /gene="gpmI"
                     /locus_tag="PLW82_01390"
     CDS             1600..3123
                     /gene="gpmI"
                     /locus_tag="PLW82_01390"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOJ45960.1"
                     /translation="MTKPVVLLIMDGWGINPKKDGNAVFLGNTPNLDRLSATFPTSRL
                     QASGEAVGIMEGQMGDSNVGHLNLGAGRIVYQDLVRLSKAVENGEFFQNPEINELMDH
                     VLAKGSALHLMGLVSPGGVHSHTNHLYALLQMAKDKGLEKVFVHAFLDGRDVPPASAK
                     AYLVELEEKMQEIGVGKIATISGRYYAMDRDNRWDRVEKTFNAIVYGKGREAASAVEA
                     LENSYKEDVTDEFVLPAVILERGKPTATVEPNDGVMFFNFRFDRARQLTRAFVDEDFQ
                     GFVRTKPDVKFLCMTRYDETIDAPFAFGPIENRNTLGEWLSKHGKRQLRIAETEKYAH
                     VTFFFNGLEEKPFELEDRMLVPSPQVATYDLRPEMSAYGITGKVIDAIESDKYDVIIL
                     NYANGDMVGHTGDLLAAVKAVEAVDSCVARVVDAVLKKDGAVLITADHGNCEQMVDYE
                     TGEPHTAHTLNEVPVILASNRDYKLRESGILADVAPTLLDIMGMEQAPEMTGKSLLR"
     gene            3167..4459
                     /gene="eno"
                     /locus_tag="PLW82_01395"
     CDS             3167..4459
                     /gene="eno"
                     /locus_tag="PLW82_01395"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOJ45961.1"
                     /translation="MTMIFDVRAREILDSRGNPTVEVEVALECGVVGRAAVPSGASTG
                     QFEAVELRDGDKSRYLGKGVENAVRNVNEKIGPEIIGMNATDQVAIDELMIKLDGTPN
                     KSNLGANAILGVSLAVAKAAAEACGLPLYAYIGGANAKELPVPMMNILNGGKHADNTV
                     DIQEFMIMPVGAKSFREALRMGAEVFHALKKVLGDKGYSTAVGDEGGFAPNLTTNEEA
                     IQVILEAVEKAGYKPGEDIVIAMDVAATEMFQEDGLYHFEGEGVTRNTDEMIAYYTDL
                     VNKYPIVSIEDALSEEEWDGWKKLTDALGKKVQLVGDDLFVTNTERLKRGIDMDVANS
                     ILIKVNQIGTLTETLDAIEMAKRAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGA
                     PSRTDRVAKYNQLLRIEEALGENVAIYRGIKTFYNIKK"
     gene            4574..4801
                     /gene="secG"
                     /locus_tag="PLW82_01400"
     CDS             4574..4801
                     /gene="secG"
                     /locus_tag="PLW82_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOJ45962.1"
                     /translation="MRVLTFLTVLDVIVAAVLIVVVALQPSKEAGLGALGGGSQMFAN
                     KGGGFEALLDKLTTWFAIAFMVISVLIALIS"
     gene            5006..5554
                     /locus_tag="PLW82_01405"
     CDS             5006..5554
                     /locus_tag="PLW82_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45963.1"
                     /translation="MLYLTREQLCEFYDVEGVQLEDALLREKQLGTIYYHESLLKELD
                     SGRYLPIVLPVMGEVLLASGSEKPPLLPFPVPRVVYESLAFDEEYLVCTLAHYKLAHV
                     SGDFSQNRFFDFKAYQYIIESILHDYDYLNPYKGHILYWIDLREELALLLQKLPLEGV
                     NDIVAFGRRIKVTKKTIGEKML"
     gene            5574..>6494
                     /locus_tag="PLW82_01410"
     CDS             5574..>6494
                     /locus_tag="PLW82_01410"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOJ45964.1"
                     /translation="MQRLKLIVILVLVVSHLSIASYFIDINIPEYKLRLYYEDELVNT
                     FPIAVGKSVTPSILGDFKIINVIKDPTWYPQGQEPVPPGKDNPLGPWWLGLDFPGYGI
                     HGNNSPDSIGKALSAGCIRMNNDDVTYLAGVIKKGTPVRLRYDVFVATYSRGQVQIAV
                     YPDIYRLGVATAENLRRFAANHGVIAESDDWTLTEFIKLSNSKFYPLPSPVDVWVNGK
                     KYKGFKIADQYAVPLAAFGDYVKYRNSNKTYYLDTQKVRSTASNKEFAYLSSLEEDFW
                     LGAEVGSGINFQIYEAYLNGQSLGRVLKKKE"
BASE COUNT         1787 a         1415 c         1696 g         1596 t
ORIGIN      
        1 ttttgatgca gaaagaaatc gaagttatgg gcaaagcact ggcaaacccc gagagacctt
       61 tcgttgctat ccttggcggt gccaaagtct ccgacaagat cggggttatt gagaacctgc
      121 tgacaaaagt agatactcta cttatcggtg gcggtatggc ttatactttc ttaaaggcca
      181 aaggatatga aatcggcaac tccctcctcg aagaggataa gatcgacctg gccaaagaac
      241 ttatggaaaa ggcagaaaag agcaatgtaa agctgctcct gccactggat aacgtagttg
      301 cagatcgctt cgcagcagac gcagagaccc aggtcgtcgc atccacctcc attccagcag
      361 gctgggaagg cctcgacatt ggtcctaaga cccgcgagct ttttgctagc gagatcaaaa
      421 aagccaagac cgtagtttgg aacggaccaa tgggcgtttt cgaaatggag ccatttgctg
      481 caggaaccaa agcagttgca atggcccttg ctgagtgcga tggcactaca atcgtaggcg
      541 gcggcgactc ggctgcagct gttgagcagc tcggctttgc cgataaggtg acccatgttt
      601 ctaccggcgg cggagcaagc ttggagtttt tggaaggtaa agaacttccc ggcgttgctg
      661 ctctcaacga caagtaaata caaccttatg gttttaagct atacctttta cccctctctt
      721 cagggagggg taaaatggtg tagtaattga tatggaaagc gctgctggtt ggctagcgct
      781 tcctaaagac atgttttttc gattttcgat tcgggtaaga gaaggaggga gaaatttgcg
      841 tactcccata atcgccggca actggaagat gaataaaaca gttgacgagg ctttagactt
      901 ggtgcgtaaa ttgaaagaat ctttgaagga cgtaaaagac cgggaaatcg tggtctgccc
      961 ttcgtttgta gctttatacg ctgttgctaa agagattgaa ggatccaaca ttaaactcgg
     1021 cgcacagaac tgttactatg aagataacgg cgcctacact ggtgaagtgt ctccggtaat
     1081 gctcaaagac attggcgtag agtacgttgt tatcggtcac tcagaaagac gctcgtattt
     1141 tgcagaaagc gatgacttgg taaataaaaa ggccaaagct ttgctgaaaa aaggtctcaa
     1201 gcccatcatc tgtgttggag aaagcttgga gcagagagaa gctggagaga cggagaacat
     1261 cgtacgcaaa caggtcttcc gcgatctgga cggtatcgaa ccttatcagt tgaaagacgt
     1321 ggttattgct tatgagccta tttgggctat tggtacgggc aaaactgcca caagcgagga
     1381 agccaataga gtgatttctc tgattcgcga caccatcaaa gagatgtact cagagaagga
     1441 agcaagtgtt ttgcgcattc aatacggcgg aagcgtcaaa cctgagaatg ccactgaact
     1501 catgactcaa agtgagattg acggagcgtt ggttggcggc gcaagtctgg atgcagaagc
     1561 atttgaagct attgtgaagt tttagtttgg aggtcactta tgactaaacc agtagtgcta
     1621 ctaattatgg acggctgggg catcaacccc aaaaaagatg gaaacgcagt ttttcttggc
     1681 aataccccaa atctagatag actgagtgca acctttccaa cgtcgagact gcaagccagc
     1741 ggcgaagcag taggcattat ggaagggcag atgggcgatt ctaatgttgg gcacctcaat
     1801 ctaggtgccg gacgcattgt gtatcaggac cttgtgcggc tatctaaagc tgtagaaaac
     1861 ggagagtttt ttcagaaccc cgaaatcaac gagctgatgg accacgtact ggcaaagggc
     1921 agcgcacttc atctcatggg tttggtttca cccggcgggg ttcactccca caccaaccac
     1981 ctgtatgctc tgctccagat ggcaaaggat aagggtctcg agaaggtttt cgtccacgct
     2041 tttttagatg gacgagacgt tcctcctgcc tctgccaaag cctaccttgt agagcttgaa
     2101 gaaaagatgc aggagatcgg cgtcggtaag atagccacta tctccggtcg ttactacgcc
     2161 atggacaggg acaatcgctg ggatcgagtt gagaaaactt tcaacgccat cgtgtatggt
     2221 aagggaagag aagctgccag cgcagtagaa gctttggaaa actcctacaa ggaagacgtt
     2281 accgatgagt tcgtgcttcc tgcagtgatt ctggagcgcg gaaagcccac agccacggtt
     2341 gagccaaacg acggagttat gttctttaac ttccggtttg atagggcaag gcagctaact
     2401 cgagcttttg ttgacgaaga ttttcaaggc tttgtcagaa ctaagcctga tgtgaagttc
     2461 ctgtgcatga ctcgctacga tgaaaccatc gatgcaccgt ttgcttttgg tccaatcgag
     2521 aacagaaaca ctttaggcga gtggctctcc aagcacggca aacggcagct tagaattgca
     2581 gaaaccgaga agtacgctca tgtcacattc ttcttcaacg gtttggagga aaagccattc
     2641 gagttggaag acaggatgct tgtgccgtct ccgcaggttg caacctacga tcttcgcccg
     2701 gagatgagtg cttacggcat aacaggtaag gttatagatg caatcgaaag cgacaagtac
     2761 gatgtgatca ttcttaacta cgcaaatgga gacatggtcg gtcatacagg cgatcttttg
     2821 gctgcggtta aagcagttga agcagttgat agctgtgtgg ctagggttgt agatgcggtt
     2881 ctgaaaaaag acggcgcagt tttgattaca gccgaccacg gcaactgcga acagatggtc
     2941 gactacgaga ccggagaacc ccatactgct catacgctga atgaagtgcc tgtgattctc
     3001 gcaagcaaca gagactataa gctcagagag tctggcatct tggccgatgt tgcgccaact
     3061 ttgcttgaca ttatgggcat ggagcaggca ccggagatga caggaaagtc gctcttgaga
     3121 tagactaacc tatagaaaac cctaatcagt caggaggtaa tttgttatga caatgatttt
     3181 tgatgtgcgc gctagagaga ttttggactc ccgcggaaat cctactgttg aagttgaagt
     3241 tgctctggag tgcggtgttg taggtagagc tgcagttcct tccggagctt caaccggtca
     3301 gttcgaagca gttgaactga gagacggaga caagagccgc tatttgggta aaggcgttga
     3361 gaacgcagtt agaaacgtaa acgagaaaat cggtcctgag attatcggta tgaatgctac
     3421 agatcaagtg gcaatcgacg aactgatgat caaactggac ggcacaccaa ataagagcaa
     3481 cttgggtgcc aacgccattt tgggcgtatc tcttgcagta gctaaagcag cagcagaggc
     3541 ttgtggtctc ccactttatg cctacattgg cggtgccaat gctaaagagc ttccagtgcc
     3601 tatgatgaac atcctcaacg gcggcaagca tgctgacaac accgtcgaca ttcaggagtt
     3661 catgatcatg ccagttggtg ccaagtcttt ccgtgaagcc ctgagaatgg gtgctgaagt
     3721 ctttcacgct ctgaagaagg ttctcggcga caagggctac agcactgccg taggtgacga
     3781 aggcggcttt gctccaaacc ttaccactaa cgaggaagcc atccaagtga ttttggaagc
     3841 cgttgaaaaa gcaggctaca aaccaggaga agacattgta atcgctatgg acgtagctgc
     3901 tacagagatg ttccaagaag acggtctata ccacttcgaa ggtgaaggcg taaccagaaa
     3961 caccgacgaa atgatcgcct actacacaga tctcgttaac aagtacccca tcgtttctat
     4021 cgaagacgct ctctctgagg aagagtggga tggatggaag aagctgacag atgcattggg
     4081 caagaaggtt cagcttgttg gtgacgacct gtttgttaca aacacagagc gcttgaagcg
     4141 cggaattgac atggatgttg ccaactccat cttgattaag gtcaaccaaa tcggcaccct
     4201 cacagagact ttggatgcaa tcgaaatggc caaacgtgca ggttacaccg ccgttgtttc
     4261 ccaccgttcc ggcgaaactg aagacaccac aatcgccgat atcgctgttg ctaccaatgc
     4321 cggtcagatc aagacaggtg ctccttccag aaccgaccgt gttgcaaagt acaaccagct
     4381 tctgagaatc gaagaagctt taggtgagaa cgtagcaatc taccgcggta tcaagacctt
     4441 ctacaacatc aagaaataat ggcactttcg ggaggtttgt tttagtggga tctagaataa
     4501 acctcccgtg ttttcctttc ctttcttgag agctgtacat tattgtggta gaatatcctc
     4561 ataattggag gtgatgcggg tgttaacttt cttaacagtg ctcgacgtta tcgtggcagc
     4621 agtacttatc gtggtcgttg cgctacagcc tagcaaagag gccggactcg gagcgttagg
     4681 tggtggctcg cagatgtttg caaacaaagg cggcggtttt gaagccctcc tagataaact
     4741 cacgacgtgg tttgccatcg cttttatggt cattagtgtt ttgattgcat taatatccta
     4801 agtcttagag gggtgaaaga aagctttcgc ccctttaaca tatcttgaaa ccttgcggtt
     4861 atactagaaa agcccagttt taggacccga cgcccaacaa aaaccgaacc tgttcttaat
     4921 atttttggtg gaggagcagt atacttttgt ggggttttga gtcaaatggc tttgcgacaa
     4981 tcaatcccaa ataggaggtc gcctagtgct atacctgaca agggaacagc tttgtgagtt
     5041 ttacgatgta gagggagttc agctggagga tgcgcttctc agagagaagc agcttggcac
     5101 catttactat cacgaaagcc tgctgaaaga acttgacagc ggtaggtatc tgccgatagt
     5161 actgccggta atgggtgagg ttttgcttgc cagcggcagt gaaaaaccac cgcttctgcc
     5221 gtttccggtg cctagggtgg tttatgagtc tctggcattt gatgaggagt acttggtctg
     5281 cacgctagcc cactacaaac tggctcacgt aagcggagac ttctcccaaa accggttttt
     5341 tgacttcaag gcatatcagt acattattga aagcattctg cacgactatg attacctaaa
     5401 cccctacaaa ggccacatcc tctactggat agacttaaga gaagaactcg cactgctatt
     5461 gcagaaactg cctttagaag gagtaaacga tattgttgcc ttcggaaggc ggataaaggt
     5521 gaccaaaaaa accataggag agaaaatgct ttaagggttc cggggtgaaa aacatgcaga
     5581 ggttaaagct tattgttatc cttgttctgg ttgtttcgca cttatctatt gccagttatt
     5641 ttattgatat taatatccct gagtacaagc tgaggctgta ctatgaagat gagctggtaa
     5701 acactttccc cattgcggtg gggaagtcgg ttaccccgtc tattttggga gactttaaaa
     5761 ttatcaatgt gattaaggat cctacatggt atcctcaagg ccaggaacct gttccaccgg
     5821 ggaaggacaa tcccttaggt ccctggtggc tggggttgga ttttccaggg tacggtattc
     5881 acggcaacaa ctctcctgat tctattggca aagcgctgtc tgcaggttgc atccgcatga
     5941 acaacgacga tgttacatat ctagcaggtg ttatcaagaa aggcacccca gtgagacttc
     6001 gctacgacgt ttttgttgcg acctacagca gaggacaggt tcaaattgca gtctatccag
     6061 acatctacag actcggggtg gccaccgccg aaaacctccg caggtttgca gcaaatcacg
     6121 gtgtgatagc cgagagtgac gactggactc ttacggagtt catcaagctt tcaaacagta
     6181 agttttatcc gttgccatcg ccagttgacg tctgggtaaa tggaaaaaaa tacaagggat
     6241 ttaaaatcgc cgaccagtac gctgtacctt tggcagcctt cggcgattac gtgaagtata
     6301 gaaacagcaa caaaacctac tatctggata ctcaaaaagt gagatcgact gcaagcaaca
     6361 aggagtttgc ctatttatcg agtctggaag aggacttctg gcttggtgct gaggtgggtt
     6421 ctggtattaa cttccagatc tacgaagcgt acttaaatgg tcagtcgtta ggcagagtgc
     6481 tgaagaaaaa agag
//
LOCUS       DAOSDA010000034         6411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_7724_length_6411_cov_2.613279, whole genome shotgun sequence.
ACCESSION   DAOSDA010000034 DAOSDA010000000
VERSION     DAOSDA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7724_length_6411_cov_2.613279"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1129)
                     /gene="floA"
                     /locus_tag="PLW82_01415"
     CDS             complement(149..1129)
                     /gene="floA"
                     /locus_tag="PLW82_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709421.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOJ45965.1"
                     /translation="MGVFGIFIIAIVLVVISAIFSFVPVGLWIAAIAANVPVSLSNLI
                     SMRLRRVPPSIVVDAFIQARKAGLSEITLADIENHYLAGGNVRDVINALIAARGADID
                     LPLERAISIDLAGRNVLQAVQMSVQPKVIETPPISAVAKNGIELIVKARVTVRANIDR
                     LVGGAGEETIIARVGEGIVTTVGSSESHEEVLENPDLISRTVLEKSLDSGTAFEILSI
                     DIADVDVGKNIGARLSMDQAAPEKNIAQAKAAERRFAAQALEQEMKAKTQEMRAKVVE
                     AEARIPEAIAEALKTGKLGVLDYYNLQNILADTKMRESIAQSDEPSEESK"
     gene            complement(1152..2444)
                     /locus_tag="PLW82_01420"
     CDS             complement(1152..2444)
                     /locus_tag="PLW82_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOJ45966.1"
                     /translation="MKRKIPRLVLAAVLFFSLALSAFADVYLIPVKGDIDLGVAGFVK
                     RVIREAERYKADLVIIEIDTLGGRIDAALEIRDSIINAKVPTATFISGRAWSAGALIA
                     LAGDELYMVPGSSIGSAETIPKDEKTISAVRREFKATAEARGKNGRIAEAMVDSDVEI
                     PDLSPKGKILNLTAREAVNHDIADGVVASFDELIQVKSLVYEDIVAKQPSRVENLVRF
                     LTDPVVSTLLLTIGFLGIIFEFFTQGWGAAGTVGLLSLTLFFGGRYLTGLAGLENVVL
                     FIVGVILMALELLVIPGFGIVGILGILGILTSIILSFENYTQAVWVLIAAFAFTALAV
                     KLLWKKLRKSPIMKKIILESALDEERGFTIASPEVKSLVGKTGTTSTVMRPSGLVEIE
                     GQRYTAQTPGDFLEKGTKIRVIEVRANRIVVEKVPEND"
     gene            complement(2450..3040)
                     /locus_tag="PLW82_01425"
     CDS             complement(2450..3040)
                     /locus_tag="PLW82_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOJ45967.1"
                     /translation="MKANEQATSRCDYTRLLLDDYVDGNLAPLVEREVKSHLVSCSSC
                     AEEAERLKKISDELSRLGTTTTDEVLLVVKKVQKAVRKDGRWCKKRRLKIAGAVAASI
                     AALLLWSTYFRPGPVSFEINGKDIAFHAMGKTVVVDASRLPGDLTVIGGDVYVTGEIE
                     GNVTSIDGRIVAKVEDGSVLSRVKRALIEIWQRIWR"
     gene            complement(3130..3693)
                     /locus_tag="PLW82_01430"
     CDS             complement(3130..3693)
                     /locus_tag="PLW82_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ45968.1"
                     /translation="MYSDQELVAKTLKGHKSAFEELVERYAKKVYNLSLRMTRDPADA
                     EDLTQEVFLRAYRSLNTYNPAYSFVNWILKIASNLCIDHLRRKGDVRDDSDELLSLEV
                     QKTHPDPESAALYSEQVSEIEKALMSLPPKYQLVMLLLYAEDLKYEQIAEILDEPIGT
                     VKTKIHRARKILKERFFSEREKDTKKA"
     gene            complement(3794..3973)
                     /gene="rpsU"
                     /locus_tag="PLW82_01435"
     CDS             complement(3794..3973)
                     /gene="rpsU"
                     /locus_tag="PLW82_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOJ45969.1"
                     /translation="MAEVKVGKNEDLDQALRRFKAKCRRAGIFAESRKHEHYEKPSVR
                     RKKKREEARKRGRSF"
     gene            complement(4024..4371)
                     /locus_tag="PLW82_01440"
     CDS             complement(4024..4371)
                     /locus_tag="PLW82_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOJ45970.1"
                     /translation="MADDCIFCKIADKTMPSDIVYEDDLVVAFRDIDPQAPVHILVIP
                     KKHISTVLTLTHEDKELVGHMFLVAASLSREQQIAQNGFRLVTNCNKDGGQTVFHLHM
                     HLLGGRSLHWPPG"
     gene            complement(4451..5737)
                     /gene="mtaB"
                     /locus_tag="PLW82_01445"
     CDS             complement(4451..5737)
                     /gene="mtaB"
                     /locus_tag="PLW82_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOJ45971.1"
                     /translation="MRTVAAYTLGCKVNQYDTDGVLSLFAKRGFTVVPFTEKADVYII
                     NTCTVTAESDKKSRQMVRRAREKNLDGIVCVMGCYTQTSSEAVKEIGADIIVGTAGRD
                     KIVDLVEAFARDKKQQVLVTEPDAFEDLPVTYHEKTRVYIKIEDGCENFCAYCKIPYS
                     RGRIRSRRPESVIEEVTRLRDSGVREVVFTGIHVGFYGKDLPGWNLARILRESLEIPG
                     IRIRLSSLDPHEVDDEIIELLEHPRFCRHLHIPLQSGNDKILKKMNRNYDTAYYRSII
                     SRLKDYAPLGITTDVIVGFPGETEEDFRKTYEFVEEMGFTRLHVFPFSHRENTPAYYM
                     PLQVPQKEKARRVKELIDLGASMMQSYHERQIGRTAEVVVEQETTLNGKRYFVGTSDD
                     YIRVYIAEPCTGLAKVKLDQTLPEESGMLGTLLETV"
     gene            complement(5734..>6411)
                     /locus_tag="PLW82_01450"
     CDS             complement(5734..>6411)
                     /locus_tag="PLW82_01450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOJ45972.1"
                     /translation="VVGDEVKHMHVLRLKVGDEVLLSDGTRLAKCLLTDLQKTRAQFE
                     LIAEVPSTEPPYEITLFQGLPKGDKLDLIVQKAVELGAAKIVPVQTSRTVVKWDEHKA
                     RSKVERLNKIALEAAKQSHRVVRPAVEELCSWQVFLDKWQEHGGLKLVFWEESTGDLR
                     DAFSETPEKVAILIGPEGGLSPEEIKELNAPTYTLGPRILRTETAPIAALACLMFGLE
                     MKGENRT"
BASE COUNT         1535 a         1711 c         1370 g         1795 t
ORIGIN      
        1 tcaaagacct ttttccagtc aggaacctgc tgctgcggac gtttttcttg gtggctcttt
       61 tttacactac tggaaaacga agcgactatt gagaatataa ccaccaacag ctttatgaga
      121 ctggatacat ccacggaatc accccgtttt atttgctctc ttcactaggt tcgtcagatt
      181 gcgctatgga ctccctcatc ttggtatctg ccaaaatgtt ttgcaggttg tagtagtcga
      241 ggacaccgag ctttccggtt ttcaaagctt cggctatggc ctctggaatc ctggcttcgg
      301 cttcgacaac cttggcccgc atctcttgag tcttggcctt catctcctgc tctaaagctt
      361 gagctgcaaa gcgcctttca gcggctttag cttgagcgat gtttttctct ggcgcggctt
      421 ggtccataga gagtctagca ccgatattct tgccaacatc tacgtcagca atatcgatgg
      481 aaagaatctc aaatgccgtt cctgaatcca agctcttttc caaaacggtc cgagaaatca
      541 agtcggggtt ttctagcacc tcttcgtggc tttcagaaga accaacggtg gtaactatac
      601 cctcaccaac gcgggcgata atcgtctctt cgcctgcacc gccgaccagt ctatcgatgt
      661 tagccctaac agtaactcta gctttaacga tcagctcgat accgttttta gcgacagcgg
      721 agatcggagg agtttcaata accttaggtt gaacgctcat ttgtacggct tggagaacgt
      781 ttcttcctgc aaggtcgata gaaatagccc gctcaagtgg aaggtcgata tcggcacccc
      841 gagccgcaat caatgcatta atgacatccc ttacgttacc gcctgcaagg tagtggtttt
      901 cgatatctgc cagtgtgatt tcggaaaggc cggcttttct cgcttgaatg aaggcgtcga
      961 ctacaatact tggcggtact ctcctaagac gcatgctaat caagttgctt aaagatacgg
     1021 gaacatttgc tgcgatagct gcgatccaaa gccccaccgg cacaaaggaa aagattgcag
     1081 aaatgacaac caacactatt gcgatgataa aaatgccaaa aactcccata cttcaccctc
     1141 ctcaaagtac ttcagtcgtt ctctggtact ttttcaacta caatacgatt agctctaact
     1201 tcaataactc tgatttttgt gcccttttcc aggaaatccc ctggtgtttg agcggtgtaa
     1261 cgctggcctt cgatttccac aagacctgac ggccgcatta cggtactggt tgttccggtt
     1321 ttgcctacca agctttttac ttctggagac gcaatggtga accctctttc ttcatcgaga
     1381 gccgattcaa gaatgatctt cttcataatc ggagatttcc tgagcttttt ccacaacagt
     1441 tttaccgcca gggccgtgaa ggcgaaggct gcaatcagta cccaaactgc ttgggtgtag
     1501 ttttcaaagc tcaaaataat gctggtaagg atgcccagga tccccaaaat gcccacaata
     1561 ccaaaaccgg gaataactaa aagttctaga gccatgagta tgactcccac gatgaacaga
     1621 acgacgtttt ccagaccggc aagtccggta agatagcgtc ctccgaaaaa gagagtcaga
     1681 cttaaaagac caacagttcc tgctgcaccc caaccttggg tgaagaactc aaaaatgatc
     1741 cctaaaaagc ctatagtcaa gagcagagtg ctcacaacag gatcggtgag aaatctcacc
     1801 aggttttcca cccgtgaggg ctgttttgca acaatatcct catataccaa acttttaacc
     1861 tgtatgagtt cgtcgaagct cgcaaccact ccatctgcta tatcgtggtt tacagcttct
     1921 ctggctgtta agttaagaat ctttcctttt ggactcaaat cgggtatttc cacatcgcta
     1981 tcaaccatcg cttcagctat tctgccgttt tttcctctgg cttcagctgt agccttaaac
     2041 tctcggcgta cagcagaaat ggtcttctcg tccttgggga tggtctctgc agacccgata
     2101 ctgcttccgg gaaccatgta aagttcatcc cctgcaaggg cgatcagtgc tccggcagac
     2161 caagcccttc cggagataaa cgtcgctgtt ggaacctttg cgttgatgat gctgtcccta
     2221 atttccagag ccgcatcgat cctgccgcct aaagtgtcga tctcaatgat gaccagatca
     2281 gctttgtacc gctctgcttc cctgatcacc cttttgacaa agcccgctac acctaagtcg
     2341 atgtcgcctt taaccgggat caggtacaca tcggcaaagg ccgagagtgc aaggctaaag
     2401 aacagtactg cggccaaaac cagacgggga atcttccgct tcaactaggt cacctccata
     2461 ttctctgcca gatctctatt agagcccgtt tcacgcggct gagtacgcta ccatcttcga
     2521 cttttgccac aattcgtcca tcgatggatg tgacattccc ttcaatctct ccagtgacgt
     2581 aaacatcgcc tccgatcact gttagatctc ctgggagacg ggaagcatca acaacgactg
     2641 tcttccccat agcgtgaaac gcaatgtctt ttccattaat ctcaaaagac acgggccctg
     2701 gtctgaagta cgttgaccag agaagcaaag cagctatgct cgctgcaact gcaccggcaa
     2761 ttttcaacct cctctttttg caccatctgc catcctttcg aactgccttt tgtacttttt
     2821 tcactaccaa caaaacttcg tcagtagtgg tagtcccgag gcgggacagt tcatctgata
     2881 tcttcttaag cctttcggct tcctctgcac aacttgagca actcactaga tgcgacttga
     2941 cttctctttc tactagcggg gccaggttac cgtccacata atcgtcaagt aaaagcctgg
     3001 tgtaatcgca cctggacgtt gcttgctcat tcgctttcac gttaggcgct gcgtatatgg
     3061 gctccgcgtt ggagcacgca gcctgaacct cctttcaaca agcacacttc cctacactct
     3121 ttagccaatt tacgctttct tggtgtcttt ttctctttcg ctgaagaagc gctctttcaa
     3181 gattttcctt gccctatgaa tcttggtctt caccgttcct attggttcgt ccaagatctc
     3241 cgctatctgt tcgtatttca agtcttctgc gtacagaagc agcattacca actggtattt
     3301 tggaggcagg ctcatcagcg ctttttcaat ctccgacact tgttcactgt atagggcagc
     3361 gctctctgga tcagggtggg ttttctgcac ttccaaactt aaaagctcat cggagtcatc
     3421 tcgaacatcc ccttttcttc tcagatgatc gatacaaagg ttggaagcga tcttaagaat
     3481 ccagttcaca aacgaatagg ccggattata ggtgttcaga gaacggtatg ctctaagaaa
     3541 cacctcttgg gtcaggtcct cggcatctgc agggtctcgc gtcattctga gactcaaatt
     3601 gtagactttt tttgcgtagc gctcaaccaa ctcctcaaaa gcggacttgt gaccttttaa
     3661 ggttttcgct acaagttcct gatcgctgta caagtcactt gcctacctcc ttctacggag
     3721 aatatgtaaa aaaggtttca gcaaaccaaa aaaacataca aaaaggcctg ccctgtgggc
     3781 aggctcctag ctactaaaac gaacgacctc tttttcttgc ttcttcacgc tttttcttcc
     3841 tgcgcacgct tggcttttca tagtgctcgt gcttgcgtga ttcggcgaag ataccagcac
     3901 gccgacactt agctttgaaa cgtcggaggg cttgatcaag atcctcgttt tttccaactt
     3961 tgacttcggc catactctcc ctcctccccg atgctactgt tcaacaacat gatcacccaa
     4021 ttcttatccg ggaggccaat ggagagaacg accgcccaaa agatgcatat ggagatggaa
     4081 gactgtttga cccccatctt tgttgcagtt tgtcactagc ctaaacccat tctgggctat
     4141 ctgttgttct ctagagagag aggctgcaac taaaaacatg tgaccgacca actctttatc
     4201 ttcgtgagta agagttaaga ctgtgctgat atgctttttg ggaatcacca agatgtgaac
     4261 tggtgcttgt ggatcgatat ctctgaatgc tacaacgaga tcatcttcgt agactatatc
     4321 gctgggcatg gttttatcag ctattttgca aaaaatgcag tcatcagcca cattgacacc
     4381 tcctctcgcg catgatcacc tttatttatt ctctttcccg ttttgaattc ctgcagaaaa
     4441 aagcatacct ttacacagtt tctaacaaag ttcccagcat tccgctttct tccggcaaag
     4501 tttgatccag cttgacttta gccaacccag tgcaaggttc ggcaatgtaa accctgatat
     4561 agtcgtcaga agttccgaca aagtagcgct tgccatttaa ggtggtttcc tgctccacca
     4621 ccacctcagc ggttctgccg atctgccttt cgtgatagct ctgcatcatg ctcgctccaa
     4681 gatcaatcag ttccttaacc cttctggctt tctccttctg gggaacctgt aggggcatat
     4741 agtaggcagg agtgttttct ctgtgggaaa acgggaagac gtggagccgg gtaaagccca
     4801 tctcttccac aaactcgtag gttttgcgga agtcctcctc tgtctcaccg gggaagccca
     4861 cgatcacatc ggtggtgatc cccagaggtg catagtcctt aagtcttgag atgatactcc
     4921 tataatacgc tgtatcgtaa tttctgttca ttttctttag gattttgtca tttccgctct
     4981 gcagaggaat gtgcagatgc cgacagaagc gaggatgctc taaaagctct atgatctcat
     5041 cgtctacttc atggggatct agtgaactca gtctgatcct gatcccggga atctccaaag
     5101 attccctcag aatccttgcc agattccacc caggcaggtc tttgccgtaa aagcccacgt
     5161 ggataccggt aaagactact tctctgacgc cggaatccct tagtctagtc acttcttcta
     5221 tgaccgactc aggccttctg ctccttattc tacctcggga ataaggtatt ttgcagtaag
     5281 cacagaagtt ctcacagccg tcttcgatct taatgtagac tctggttttt tcgtgatagg
     5341 taacaggcag gtcttcgaag gcatcaggct ctgtaaccaa aacctgctgc tttttgtctc
     5401 tggcaaaggc ttcgaccaaa tcgacgatct tgtcccttcc tgcagttcct acaataatat
     5461 ctgcaccgat ctctttcacc gcttcggacg acgtttgggt gtaacagccc atcacgcaga
     5521 caattccatc caggtttttc tccctggctc tgcgtaccat ctggcgggat tttttatcgc
     5581 tttctgcagt tacggtgcag gtgttgatta tataaacatc ggctttctca gtaaagggga
     5641 ctacagtaaa acccctttta gcaaagagac tgaggacacc gtctgtatcg tattggttta
     5701 ctttgcagcc aagggtgtag gcggcaactg tcctcaagta cgattctctc ctttcatctc
     5761 aagcccgaac ataaggcagg ccagcgctgc aattggcgcc gtttcggttc gcaggattct
     5821 cggcccgaga gtataggtag gagcgttcag ctctttgatc tcctctggag aaaggccacc
     5881 ttcaggaccg atcaaaatcg cgactttctc gggtgtttcg ctgaaggcat ctctaaggtc
     5941 tccggtgctt tcctcccaaa agaccaattt aagtccgccg tgctcctgcc atttatccag
     6001 aaaaacctgc cagctgcaca gttcctctac agcagggcga accactcgat ggctctgctt
     6061 agcagcttcc aaggcaatct tgttcagccg ctccacttta cttcgagctt tgtgctcatc
     6121 ccactttacc acggtgcggg aggtttgtac agggacgatt ttcgccgctc cgagttccac
     6181 tgctttctga acgatcaggt caagtttatc ccctttaggc agaccctgaa acagggtgat
     6241 ctcataggga ggctcggtag aaggaacttc ggctataagc tcaaattgag cccttgtctt
     6301 ctgtagatct gtaaggaggc actttgccaa cctagtaccg tctgaaagga gaacttcatc
     6361 gccgactttc agccggagga cgtgcatatg cttaacttca tctcccacaa c
//
LOCUS       DAOSDA010000035         6384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_7765_length_6384_cov_2.321062, whole genome shotgun sequence.
ACCESSION   DAOSDA010000035 DAOSDA010000000
VERSION     DAOSDA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7765_length_6384_cov_2.321062"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLW82_01455"
     CDS             complement(<1..243)
                     /locus_tag="PLW82_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOJ45973.1"
                     /translation="MERIGDYLYTFDGEGKEKHVPVIKAPEKVKAGEPFEVTVIVGDE
                     VPHPNTVEHHIKWIALFAKPEKGNIVHIATFDMGPTY"
     gene            complement(343..1281)
                     /locus_tag="PLW82_01460"
     CDS             complement(343..1281)
                     /locus_tag="PLW82_01460"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOJ45974.1"
                     /translation="MATTITANEAGQRLDRYLRKRFSSQALGDIYRLIRTGQVKVNGR
                     KAKAEYRLEEGDVLTLPDLNEERKEAFVNVPCDFEIVFEDENLLIVNKPAGLLVHPDK
                     TEMKRTLINQALAYLYRKGEYDPQSSSTFTPALCHRLDRNTSGLVVIAKTYPALQDMT
                     ELIRQKKVKKFYLCLVEGRLSKPGIVELKLEKDESKNQVKVARSGLEATTSYVPVEIF
                     EGYTLLEAEIVTGRTHQIRVHLSGIGHPLVGDLKYGKRAANEQFRRKYGLTRQFLHAY
                     KLILHSKGTSLEYLEGRTFSSDLPEDLQKVLILLED"
     gene            complement(1431..3023)
                     /locus_tag="PLW82_01465"
     CDS             complement(1431..3023)
                     /locus_tag="PLW82_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45975.1"
                     /translation="MKKIWTLSLLILLIIPVFSQDVSYNQYYNLYKNRVVRTEYAEKL
                     DVHSYVGEEPDAAPDEYFLQDKKPIILPKTGATWKVSVPESGLYTFFFEYLFYPANGE
                     TEKLTVVIDGQLPFAEAESVLPPRFFRNGVADREKPNRNSTVAPLPGIIWQKRYAGDT
                     SGMSDYPMKFYLTAGEHEITLIPQGGYWYLNSFEVAAYQAPPTLEEYRKLYPVEFTAA
                     IDTVITIDGAYYTYQYGEPDAIIYMDLEANPTRDYNALGRWRNPGNGVIWEFEVEETG
                     FYEIAFRYALGSIPLVEIPLGNMSQKGFVHSERMLLVDGKVPFQEAGLIVFNPTDGGR
                     SRYTNYWYTYSPTFNVKGSRLNFELFLEKGTHTIELIPTLQMRLPIINLLQEIFQRIE
                     KLESDYKEALGEDPNPNLSYPLKQETVDDIRALLSDVLLAIRRVANFSRNSRVESSLN
                     DVLYRLTNLALLTAIDTGDMVEITRIKENLRRTINNAYYTPLNLDAILLYSPRNEKII
                     RNERNDSFWGMLSNYFKYRVKI"
     gene            3305..4255
                     /locus_tag="PLW82_01470"
     CDS             3305..4255
                     /locus_tag="PLW82_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOJ45976.1"
                     /translation="MSKYFIGLDLGGTKIATGIVNSKDELLASVKVPTQAEDGWKIIT
                     ERMGDTIAEVMQKAGVKPTEVKAIGVCSPGALDIPNGIVIRSPNLRWFNVPLKKIIED
                     RFNIPTYFDNDANVAGLGENRYGAGVGSKHMLYMTISTGIGGGIIIDGKIYYGATFGA
                     GEIGHITLDKNGPLCGCGNHGCIEAIASGPAIARNAKERLHEHPESLLHHYTNITARE
                     VSEAAHAGDQYALSVVETAARNIGVVLAGLINTLNPDCVVIGGGVSNMGDILFKPLIA
                     EVKERAIPSFVENLKIVPAKLGGDVGVMGAIALAQSQGNV"
     gene            complement(4503..4937)
                     /locus_tag="PLW82_01475"
     CDS             complement(4503..4937)
                     /locus_tag="PLW82_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOJ45977.1"
                     /translation="MQTKLKEALHQKGFRVTPQRQIILELMMNLDGRHLTADEVYSLA
                     RERTDDISLATIYRTLELFVEMNLLRRFDSGDGVFRYEIDTSQSEHYHHHLICLECGK
                     IIEFNDDLLDELESRIAEKGFEIADHCLRFFGYCQDCKKKKR"
     gene            complement(5013..5363)
                     /gene="rnpB"
                     /locus_tag="PLW82_01480"
     ncRNA           complement(5013..5363)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLW82_01480"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5413..5997)
                     /locus_tag="PLW82_01485"
     CDS             complement(5413..5997)
                     /locus_tag="PLW82_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45978.1"
                     /translation="MKNVALKKTIVIFFAVLLFGVISSAAEMQVMEIPTAHTLRRNDG
                     NIVVWSNLSGFSARAYFSPVENLDFFVTLIGRPNYFQVFGGAKWQFLKEGDKLGALAI
                     GTDSIYVVMSYGLAPGTYGHIGVGAGDVSTVFAGLDYVVNLGSGAPPVKVMVEYNNGL
                     NFGFSAEVAPALSVGLAIKNLDRVSLGATVRFAL"
     gene            complement(6018..>6384)
                     /locus_tag="PLW82_01490"
     CDS             complement(6018..>6384)
                     /locus_tag="PLW82_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOJ45979.1"
                     /translation="TVDYLRSTYKDLNVKYFGPEMVVDDEISLEWARIPHFYLNFYVY
                     QYATGYSSAVALAMNILKVGTPAVEGYLDFLKAGSSKYPVDILAHAGVDMSTPKPIVK
                     ALEVFEQTLDELEDLAFSE"
BASE COUNT         1549 a         1625 c         1510 g         1700 t
ORIGIN      
        1 ataagtcgga cccatgtcga atgttgcgat gtgtacgatg ttgccctttt ctggtttggc
       61 aaaaagggcg atccacttaa tgtggtgctc tacggtgtta ggatgaggta cctcatcgcc
      121 aacaattaca gttacttcaa agggctctcc ggctttgacc ttttcaggtg ccttgattac
      181 cggaacatgc ttttcttttc cctcaccgtc aaaggtgtat aggtagtccc caattctttc
      241 cattctaaaa tcctcctcct cggtgtaata aacactacct ctctaatatc cagattttaa
      301 gaaaataaac attaaaatcc acaatacgtc ccaatcctaa cgctagtctt ccaggagtat
      361 cagcactttc tgcaggtctt ccggaagatc actggaaaag gtcctgcctt caagatactc
      421 cagtgaagta ccttttgaat ggagaattag cttgtaggcg tgaagaaact gcctggtcaa
      481 accgtacttt ctcctgaact gctcattggc cgcccgcttc ccgtacttca gatcgcccac
      541 taaagggtga ccaataccgg acaggtgaac gcggatctgg tgggtccttc ctgtaacaat
      601 ctctgcttct aaaagggtgt aaccttcaaa aatctctaca ggaacgtagc tggtggtggc
      661 ttcaagtcca cttcgggcca ctttgacctg gttcttgctt tcgtcttttt ccagcttcag
      721 ctctacaatt ccgggcttgg atagtctccc ttccaccaga cacaggtaga actttttcac
      781 cttcttctgg cgaatgagtt cagtcatatc ttggagagct ggataggttt tggcaattac
      841 caccaagccg ctggtgttgc ggtccagtct gtggcagagg gccggagtga atgtggagct
      901 tgattgggga tcgtactcgc cctttctata cagataagcc aaagcctgat tgatcagggt
      961 ccttttcatc tcggttttgt cagggtgaac caagagccct gcaggcttat tgacgattag
     1021 gagattctcg tcctcgaaga cgatctcaaa atcacaaggc acgttgacga aagcctcttt
     1081 ccgctcctcg ttgaggtctg gcagggtaag tacatctccc tcctccaagc ggtactctgc
     1141 tttggctttc cttccgttaa ctttaacctg gcctgtacgg atcaacctgt agatatctcc
     1201 tagagcttgg ctggaaaacc tctttctgag gtaccggtcc agccgctgtc cggcttcgtt
     1261 tgcagtgata gttgttgcca tattaactcc acagttcctt tcatcttctg acttcccatt
     1321 gggttccaag gattcttgtt accctttttc acggttccgc acacgctcca cttccgtaaa
     1381 acttgcgcat gcggttggaa aagcctgacc agaaggccag gcttcataaa tcagatcttc
     1441 actctgtact taaagtagtt ggagagcatt ccccagaaag agtcgtttct ttcgtttctg
     1501 atgatcttct cgtttctcgg tgaatacaga agaatggcgt caagattgag cggtgtgtag
     1561 taggcattat ttattgttct tctgaggttt tcctttattc tggtgatctc taccatatct
     1621 ccagtatcga ttgcagtaag gagcgctaga ttggtcagac ggtagagaac atcattcagg
     1681 gaagactcta ctcgggagtt tctggagaag tttgcaactc tccttatcgc cagcagtacg
     1741 tcgctaagga gtgctctgat gtcatcgacg gtctcctgct tcaaggggta gctcaagttg
     1801 ggattgggat cctcgccaag agcttcctta tagtcacttt caagcttttc gattctctgg
     1861 aagatctcct gcaggaggtt gatgataggc agtcgcatct gcagggtggg gatcagctct
     1921 atagtgtgag ttcccttttc caaaaacagc tcgaagttca gcctgctgcc cttgacgttg
     1981 aaagtcggtg agtaggtgta ccagtagttg gtgtagcgac tccgtccgcc gtctgtgggg
     2041 ttgaatacta tgagccctgc ttcctggaac ggtactttgc cgtctacaag cagcattctc
     2101 tccgagtgaa cgaagccctt ctgactcatg ttgccgaggg gaatctccac aagaggtata
     2161 ctgccaagag cgtagcgaaa cgctatctcg taaaaaccgg tttcttctac ttcaaactcc
     2221 catatgacgc cattgcccgg gttgcgccac ctgccgagag cgttgtagtc tctcgtgggg
     2281 tttgcttcaa ggtccatgta aatgatggcg tcaggttcgc cgtactgata ggtatagtat
     2341 gctccatcga tagtgatgac agtatcgata gccgcagtga actctacggg gtagagcttg
     2401 cggtactcct caagtgttgg cggagcttga tatgctgcca cttcaaaact gtttaagtac
     2461 cagtatccgc cctgggggat cagggtgatc tcgtgctcac ctgcggtgag gtagaacttc
     2521 ataggataat ccgacatccc cgaggtatct ccagcgtagc gcttttgcca gataatcccg
     2581 ggaagcggcg caacagtcga attgcggttg ggtttttcgc ggtctgcaac tccgttgcgg
     2641 aaaaaccttg ggggcagaac gctttccgcc tcagcaaacg gcagctggcc gtcgatcaca
     2701 acagtgagct tttctgtttc tccgtttgct ggatagaata ggtattcaaa gaaaaaagta
     2761 taaagccctg attcgggaac actcaccttc caagtagccc cggttttcgg taagataatc
     2821 ggcttcttat cttgaagaaa atactcgtct ggagctgcat cgggctcctc gcctacatag
     2881 ctatggacat cgagcttttc agcatactct gttctcacga ctctgttctt gtacaagttg
     2941 tagtattggt tgtacgatac atcttgtgaa aaaactggta taatgagaag gataagtaat
     3001 gaaagagtcc aaatcttttt catgtccgta atcgtcctcc ctccgacgat tacttcaccc
     3061 cccaatcgta aattgacgtc taccagatta gtttcagtat aattttctcg attccttcaa
     3121 aggaagacaa cctttttcat tgtattatta aacagtggtc aactgcgacc aatcatagta
     3181 taattgtgcg gaacagcatt gtggcagtgg atgggcgcca aaagagcttc tttggcggca
     3241 cgcaaaatca cagcccgaac gtttataatg cacatccccc ccatagatgc aaggaggcag
     3301 tagaatgtct aaatacttca taggactcga cctaggtgga acaaaaatcg ccacaggcat
     3361 agtcaacagc aaggatgagc ttcttgcgag cgtaaaggtc cccactcaag cagaagacgg
     3421 ctggaaaatc atcaccgaac gcatgggaga caccatcgcc gaggttatgc aaaaggccgg
     3481 agtaaagccc actgaggtaa aagccatagg agtgtgctcg cctggcgctt tagatattcc
     3541 caatggcata gttatcagat cgccgaactt gaggtggttc aacgtacctc tgaagaagat
     3601 cattgaagac cgtttcaaca tccccactta tttcgataac gacgctaacg tagcaggact
     3661 cggcgaaaac cgctacggag ccggagtagg ttcaaagcac atgctctaca tgaccatttc
     3721 caccggtatt ggcggcggga tcatcattga cggcaagatc tactacggcg caaccttcgg
     3781 agccggcgag atcggccata tcactctcga taagaacggt ccgctctgcg gttgcggcaa
     3841 ccacggctgc atcgaagcca tcgcatctgg acctgctatc gccagaaacg ccaaggagcg
     3901 cttgcacgaa catcctgaaa gcctgctgca ccattacacc aacatcaccg cccgcgaggt
     3961 ttctgaagca gctcacgctg gcgatcagta cgcactgagt gttgttgaaa cagcagcaag
     4021 aaacatcggc gttgttttgg ccggccttat caacacactt aatcctgact gcgttgttat
     4081 tggcggcggt gtttcaaaca tgggagatat cctcttcaag ccgctgattg cagaagttaa
     4141 agagcgtgct attccatcgt ttgtagaaaa cctcaagata gtgcctgcaa agctcggagg
     4201 cgacgttggc gtaatgggag ccatcgcgct agctcaaagc caaggaaacg tataagaaca
     4261 cgccaaagaa tctcttacac tgttttttcg atgaaccctc atcgaatact cttcttcgga
     4321 agattattaa gacagccaag aagtgttcat tagccgttca tcacggatac gtagaataga
     4381 gtaaaacaag cagcgtctcg aggcgctgct tgtttttgga ctgagacaac ttgcttgctt
     4441 ttgaatgcga aaaaccccac agagacttta cgtctcggtg gggtgtcttt cttcacctga
     4501 acttatcttt ttttcttctt gcagtcctga cagtagccaa aaaagcgcag gcagtggtcg
     4561 gcgatctcaa aacctttttc cgcaatacgg ctttccagct catctaaaag atcgtcgttg
     4621 aattcgatga tcttgccaca ttccaagcat attaggtggt ggtgatagtg ttccgattga
     4681 ctggtgtcaa tctcgtagcg aaaaaccccg tcacctgaat caaagcggcg cagaaggttc
     4741 atttccacga acagctcgag ggttctgtag atggtcgcaa gactaatgtc gtctgtacgc
     4801 tctctagcca gactgtaaac ctcatcagca gtaaggtgtc ttccgtcaag attcatcatc
     4861 agttccaaga taatctgccg ctgaggggtt acgcgaaaac ctttttggtg cagggcttct
     4921 tttaacttgg tttgcatttt atcaccccaa gatttggcat tgaaagggga aatacaaacg
     4981 acgccacaag aggtagcgtc gcttacatgt ttctcacaac caaccctata agccgggttc
     5041 tgtacccgct aaagcgggcg atgatcatct atctgggcta gcagttgcct actagctcta
     5101 gcggcctaac ccaagggaaa gacgggccat cttatctccc ttctatttgg ccttgctcca
     5161 ggtggggttt accaagccgt ttggtcaccc aaaacgctgg tgcgctctta ccgcaccttt
     5221 tcacccttac ctgccaatgg caggcggttt gctctctgtg gcactttcct tgaggtcacc
     5281 ctcactggat attatccagc accctgccct atggagcccg gactttcctc accgcatggc
     5341 ggcgcgatca tccaggttgg ttgttgaggc ctctttaaca gtatcattta ttgagttttc
     5401 tgtcaaactc acctacagcg caaatctcac cgttgcaccc aaactcaccc tatccaggtt
     5461 ctttattgcc agacctacgc tgagtgcagg cgccacttcg gcgctgaagc cgaaattgag
     5521 tccgttgttg tattccacca taacctttac cggaggcgct ccggatccca ggttcacaac
     5581 gtagtccaaa ccggcaaata ccgttgacac atcaccagct ccgaccccga tgtgtccgta
     5641 cgttcccggt gcaaggccat aggacatcac cacatagatg gaatcggtgc cgatggccag
     5701 ggctccgagc ttatctcctt ctttcaagaa ctgccactta gctccgccga agacctggaa
     5761 gtagttcggc ctgcctatga gggtcacgaa gaagtcgagg ttctccacag gggaaaagta
     5821 agctctggcg gaaaagcccg agagattgct ccagaccacg atgttaccat cgttccttct
     5881 caaagtgtgt gctgtgggga tctccatcac ctgcatctca gctgcactgg agataacacc
     5941 gaagagaagt acggcgaaaa agatgactat tgttttcttt aaggcaacat tcttcatgca
     6001 ataacccccg taaagtctta ttctgaaaac gccagatcct ccagttcgtc taaagtctgt
     6061 tcgaagactt caagcgcttt tacgatcggc tttggagtgc tcatatccac gccggcgtga
     6121 gctaggatat ccactgggta cttgctggac cctgctttga ggaagtccag gtacccttct
     6181 actgcaggcg tgcctacttt tagaatgttc attgccagtg ctaccgcaga cgagtatccg
     6241 gtagcgtatt gatagacata gaagttgagg tagaagtgag gaatgcgggc ccactccaga
     6301 ctgatctcat cgtctacaac catctccggt ccgaagtact tgacgttgag atctttatag
     6361 gtgctcctca gatagtcaac agtc
//
LOCUS       DAOSDA010000036         6290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_7909_length_6290_cov_3.039134, whole genome shotgun sequence.
ACCESSION   DAOSDA010000036 DAOSDA010000000
VERSION     DAOSDA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7909_length_6290_cov_3.039134"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLW82_01495"
     CDS             complement(<1..543)
                     /locus_tag="PLW82_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015891990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOJ45980.1"
                     /translation="MIGEILGNRYEIKKLIGEGGMALVYLAQDLLLDRPVAIKLLRSE
                     YSSDQEFVERFQREAKSAAKLCHPNVVNIYDVGETENSHYIVMEYIDGISLKDLIKKE
                     GMLSVEDSLKIAEQICSALDHAHKNLIVHRDIKPHNIMITKQKHVKVMDFGIARAVSS
                     ATITRTGVVLGSVHYFSPEQA"
     gene            complement(561..1253)
                     /locus_tag="PLW82_01500"
     CDS             complement(561..1253)
                     /locus_tag="PLW82_01500"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOJ45981.1"
                     /translation="MQVVAKSEKGLVRKTNEDRFLATESLLAVADGMGGHLAGDVAAQ
                     IAIEKLSEFTAEGDVLSNLKALFLLTNRTIFEQGKYNKEQEGMGTTLTVCCVQGSKGY
                     FGHVGDSRAYLVRDGQVRQLTQDHSYVNQLVVLGTLSQEEAKNHPQRNVLLRALGAQE
                     DIEVDLFEVDFQPGDIVLLATDGLVTDLDKEVLEEILLSEISLEEMADRLLEAALASG
                     GRDNVTVVLGQV"
     gene            complement(1270..2274)
                     /gene="rlmN"
                     /locus_tag="PLW82_01505"
     CDS             complement(1270..2274)
                     /gene="rlmN"
                     /locus_tag="PLW82_01505"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOJ45982.1"
                     /translation="MDFFSLTKQELEEYLLMRGLPKYRALQLLHWVYKKGITDTEEMT
                     NLPKSMKGEALGLSILPLKEELIQRSVDGTEKALLQLEDGEYIETVYIPDGLRATVCF
                     STQVGCAMGCSFCATGKSGFSRNLSSGEIVGQLYYWKYVRKLPVTNAVAMGQGEPLAN
                     WENFKKAIAIINDPDAFSLGARMITVSTCGIAPRIRELGRLPWQINLSVSLHAPTDEL
                     RSQLMPINNKYPLKELIEAVKEYIEATNRRVTFEYTVVPGLNDGPENARLLVGLLRGL
                     LCHVNVIPVNPLEGKGEDPSAAEEFVRLIKSQLNVTLRRSRGSDIDAACGQLKRKVFE
                     "
     gene            2306..2590
                     /locus_tag="PLW82_01510"
     CDS             2306..2590
                     /locus_tag="PLW82_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ45983.1"
                     /translation="MSKTYFITAYMEHLLKQGLKHEEVYVGDASRFLRYLLSTAPEDA
                     VQKFITASGTSPSYHRRLSSSLNRFLAFAQSHLDISSFSADVDSEDREAL"
     gene            complement(2544..3896)
                     /gene="murC"
                     /locus_tag="PLW82_01515"
     CDS             complement(2544..3896)
                     /gene="murC"
                     /locus_tag="PLW82_01515"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOJ45984.1"
                     /translation="MWYHYVGIGGIGMSALAAIMLSKGHKVTGSDLKENFQTEQLKNQ
                     GATIFVGHREENVEAPDVVVYTSAVPFDNPERVAARKKHIKEIHRSENLAEILNGHYG
                     IAISGAHGKTTTSGMTTYMLRQVGKDPTGIIGGYIPQLQGNFVVGMSDLVVAESDESD
                     GSFLNYYPHIGSVSSVDADHLENFQYNYEKLYEAYKKFVANIKDLAIICADEKATLDL
                     AEHAKAKVITYGLTAGDLRAYDIVEGPVTTFRVRLKDKDLGIFSLKLPGKHNVANSLV
                     AISVALELGLDMDAVKHALSEFSGIGRRFEIRYQVGDTLIVDDYGHHPKEIVATLDAA
                     RNRYPEKEIWLCFQPHRYSRTKLLWGEFIKALSHADKVVVLGIYAPPPEQPILGVSGA
                     ELADEVAKLGKSAFYAETLEDAAELVKEKAPKGALIIAMGAGSVTKLPDLLNLRLQRS
                     "
     gene            complement(3947..>6290)
                     /locus_tag="PLW82_01520"
     CDS             complement(3947..>6290)
                     /locus_tag="PLW82_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOJ45985.1"
                     /translation="NWQTVARFLGRNPFGIEDPIFHMDVSFYVFVLPLIQGLYVTAFA
                     VAVFTLLFTGVFYLATRSITFYKRRVYLGDRARLHLLLLIILIVALAVLGLQINSYEL
                     LRSPTGAVYGAGYSDVYGRLVVNRILQILCVLAVFVFALDLFRREYKWTVWTLLVLVA
                     VGILGQFAAPILQKVVVEPNELNKERRFIEHNIGFTRFAYGLDEVVSAQFEPALAFSS
                     DDWIDNTEVLDNVRLWDWRALEKSFSQLQELRYYYKMGDIDIDRYQVDGKTTQMALSV
                     REIDSNYLTGTARSWVNKHLVFTHGYGFVMSSIHGVTPQGIPEFLVRDIPPKGIIDVT
                     RPEIYFGERTNDYVIVKTRQKEMNYPSGEDNVYTTYQGDGGVKVGGLFGRLLFALKFG
                     TSKILFSSDITSDSQILYYRNITERVKRLAPFLILDPDPYAVTVEGGIYWLMDAYTTT
                     SYFPYSQPISGLGNYMRAAFKVVVNAYTGSVDIYLYDEADPIGRAYKNVFPNLIKPKS
                     EFPQEFAAHIRYPEQLFLAQAQILQTYHMTNPEVFFNKEDVWDVPQEIYQGQTVRMDP
                     YYTMVQFPGENRAEFVLMLPFTPRGKQNMVSWLAGRSDGDNYGELVLYRFPKDRVIYG
                     PMQIEARINQDAEISQMLNLWNQQGSSVQRGNLLVLPLNDNILYVEPVYLQASQGQIP
                     ELRLVVLSDGNNIIYGRTFREALDRIQSLVTDETALPEDGYKTDDFAETKDADYAELA
                     RLAWEAFLESEEALQSGPDWERYGRAQAKLRELLARLNAN"
BASE COUNT         1497 a         1649 c         1507 g         1637 t
ORIGIN      
        1 agcttgctct ggcgagaagt aatgcaccga accgagcacc acgccggttc tggtgatagt
       61 tgcagaactc acagctctgg caatgccgaa gtccatgacc ttgacatgct tttgtttggt
      121 gatcatgatg ttgtgaggct tgatgtctct gtgaacgatc aggtttttgt gggcatgatc
      181 aagagcagaa cagatctgtt ctgcaatttt caaactatct tcaacagaca gcatgccttc
      241 tttcttaatt aagtctttaa ggctgatgcc gtcaatgtat tccataacga tataatgact
      301 gttttcggtt tcaccaacat cgtagatgtt tacaacgttg ggatggcaga gtttggctgc
      361 tgacttggct tctcgttgaa accgctccac gaactcttga tccgaagagt actctgaacg
      421 gagcagcttg atagccactg ggcgatcgag aagcaggtcc tgggcaaggt aaactagtgc
      481 cataccgcct tcgccgatca gctttttaat ctcgtagcga ttcccaagga tctctccaat
      541 caagagactc atctcctatc tcaaacttgg cctagtacaa cagtgacgtt atccctgcca
      601 ccagaggcca aagcagcttc aagcagtctg tctgccattt cctctaaact gatttcacta
      661 agcagtattt cttcgagaac ttctttatcc aaatcggtta caagcccgtc tgttgccaga
      721 agaacgatgt cgccgggttg aaagtccact tcaaaaagat ctacctcgat atcctcttgg
      781 gcaccgaggg ctctaaggag aacattgcgt tgcggatggt tcttggcctc ttcctgcgaa
      841 agagttccca aaacaaccag ctggttgacg taggagtggt cttgtgtaag ctgacgaact
      901 tggccatccc gtacgagata cgctctgctg tctcccacgt gaccgaagta acctttgctg
      961 ccttgaacac agcaaacggt gagggttgtc cccatccctt cctgttcttt attatactta
     1021 ccctgttcga aaatagtacg gttagtgagc aaaaaaagag ccttcaggtt gctcaaaacg
     1081 tcaccctctg cagtgaactc cgatagcttt tcaatggcta tttgagctgc aacatcacct
     1141 gcaaggtgac cacccatacc gtcagccaca gccagcagag actctgttgc tagaaaccta
     1201 tcttcattgg tttttcggac caatcccttt tcacttttag ctacgacctg cactgtagtc
     1261 accagccttt tactcgaaca cttttctttt gagttgcccg caagctgcat ctatatcaga
     1321 gcctcttgag cgcctcaaag ttacattcag ttggcttttt atcagcctta caaactcttc
     1381 ggcagctgaa ggatcttcac ctttcccctc cagcggattt acgggaatta cattgacgtg
     1441 gcagaggagt ccacgcaaga gccccacaag gagccttgca ttttcggggc cgtcgttgag
     1501 accgggaacc acagtgtatt caaaggtcac tcgcctattg gtcgcctcta tatattcctt
     1561 gacagcctct attaactcct ttaggggata tttgttattt atgggcatca gctgggagcg
     1621 gagctcgtct gtcggtgcgt gcagagacac cgacaaattg atctgccacg gcaacctgcc
     1681 aagctcccta atccttggcg ctatgccgca ggtggacacg gtgatcatcc ttgcccctaa
     1741 actaaaggca tctggatcgt tgatgatggc tatagccttt ttaaagttct cccagtttgc
     1801 cagaggttct ccctgcccca ttgccacggc gttagtgaca ggaagcttcc tgacgtactt
     1861 ccagtagtag agctgtccca caatctctcc cgaggaaagg ttcctactaa aaccgctctt
     1921 ccccgttgcg caaaagctgc agcccatggc gcagcctacc tgagtggaaa aacacactgt
     1981 agctctcaaa ccgtcgggaa tgtagacggt ctcgatgtac tcaccgtctt caagctgaag
     2041 gagagctttt tccgtgccat ccactgacct ttgtatcagt tcctctttaa ggggcagaat
     2101 actaaggcca agagcctcgc ctttcatgga tttcggcagg tttgtcatct cttcggtatc
     2161 ggtgatgcct tttttgtaca cccaatgcag aagctgcagc gcccggtact tcgggagccc
     2221 ccgcatcagc agatactctt caagttcttg tttggtcaaa ctaaaaaaat ccaaaatcca
     2281 accccctttt gaggtgaaaa taatggtgag caaaacgtat ttcattacgg cctacatgga
     2341 acacctgcta aaacaggggc taaaacacga agaggtctat gtaggagatg ccagcaggtt
     2401 cctgagatat ctcctgtcca cggctccaga ggatgcagtg cagaagttca tcaccgcttc
     2461 cggaaccagt ccatcctacc acaggcgcct gagctcatct ctcaaccggt ttctggcttt
     2521 tgcccaaagc catctggata tctctagctt ctctgcagac gtagattcag aagatcggga
     2581 agctttgtaa cggaacctgc acccatagca atgatcaaag ctccttttgg ggctttttct
     2641 ttgaccagtt cggcagcgtc ttccaacgtt tcagcgtaga aggcgctttt tccaagcttt
     2701 gccacctcgt cggcgagctc tgccccgctt actccaagaa tgggttgctc aggaggaggt
     2761 gcataaatcc ccaaaaccac aactttgtca gcgtggctga gtgctttgat gaactcgccc
     2821 cacagaagct tggtgcggct gtagcggtgg ggttggaagc aaagccagat ctccttttcg
     2881 gggtagcggt ttctagcggc atccagtgtc gccacgatct ctttggggtg atgcccgtag
     2941 tcatcgacga tgagtgtgtc tccaacttga tagcggatct caaaacgcct gccaatcccg
     3001 gaaaactcac tcagtgcgtg cttcaccgca tccatatcca gcccaagttc cagggctacg
     3061 gatatagcca ccagcgagtt ggccacattg tgttttcccg gaagcttcag ggaaaatata
     3121 cccagatctt tgtcttttaa gcgcactcta aaagtcgtaa cagggccttc aacgatgtcg
     3181 taagctctga gatctcctgc agtgagcccg taggttataa cttttgcctt tgcgtgttct
     3241 gccagatcca gggttgcctt ttcgtctgca cagattatgg ccagatcctt aatgtttgcc
     3301 acaaactttt tgtaggcttc ataaagcttc tcgtagttgt attggaagtt ttccagatgg
     3361 tccgcatcaa cgctggagac agaaccgatg tggggatagt agttaaggaa gctgccgtca
     3421 gattcgtcgc tctcagcaac aacaaggtcg ctcatgccca caacaaagtt cccctgcagc
     3481 tgtggaatgt atcccccgat gatcccagtg gggtctttcc ccacctgccg gagcatgtag
     3541 gtggtcattc ccgaggtggt tgtcttgccg tgagctcctg atatggctat gccgtaatga
     3601 ccgttgagga tctctgctag gttttccgat ctgtggatct ctttgatgtg tttctttcta
     3661 gctgcgactc tttctggatt atcgaaaggt acagcagaag tgtaaactac aacatcgggc
     3721 gcttctacgt tttcttcgcg gtgtccgacg aaaatagtgg ctccctgatt tttcagctgt
     3781 tcggtctgga agttttcctt caggtccgaa ccggtgactt tatgcccttt agacagcata
     3841 atggcagcta gggcactcat gccaattccg ccaataccta cataatgata ccacatattt
     3901 tcacctcata ctgaagcccg gcaagtgccg ggctggaata actttatcag ttggcattta
     3961 gcctagctag aagctctctc agctttgctt gggccctgcc gtacctctcc cagtccgggc
     4021 cgctctgcag cgcttcttcg ctttctaaaa atgcctccca ggcaagcctg gcaagttctg
     4081 cataatctgc atccttagtc tctgcaaaat cgtcggtctt atatccatct tcaggcaaag
     4141 ctgtctcgtc ggtgaccagg ctttggatgc ggtcaagagc ttcccggaac gtcctgccgt
     4201 agatgatgtt gttcccatcg gagagcacca ccagtctgag ctcgggaatc tgtccctggc
     4261 tggcttggag gtagaccggt tctacgtaaa ggatgttgtc gttgagaggc agcaccagga
     4321 ggttgccccg ctgaacagac gagccctgct gattccaaag attgagcatc tgggagatct
     4381 cggcgtcctg attaattcgg gcctcaatct gcataggacc gtagataacc cggtctttcg
     4441 ggaacctgta caataccagt tcgccgtagt tatctccatc ggatcttcct gcaagccagg
     4501 agaccatgtt ctgcttgccc cttggagtaa acggcagcat cagaacaaac tccgctcggt
     4561 tttctcctgg gaactgcacc atggtgtagt acggatccat tctgacggtc tgcccctggt
     4621 agatctcctg gggcacatcc cagacgtctt ctttgttgaa gaaaacttcg gggtttgtca
     4681 tgtggtaagt ttggagaatc tgggcctgtg ccaggaacag ctgctccgga tagcggatat
     4741 gcgctgcgaa ctcctgggga aactcggact tgggtttgat cagattggga aacacgtttt
     4801 tataggctct gcctattgga tccgcttcgt cgtagaggta gatgtcgaca ctgccggtgt
     4861 aagcattgac gacgacctta aacgccgctc tcatgtagtt gcccagaccg gagataggct
     4921 gcgagtatgg gaagtagctt gtggtcgtgt aggcatccat caaccagtat atgccacctt
     4981 ctaccgtaac ggcataggga tccggatcca agatcaggaa aggtgcaagt cgcttgaccc
     5041 gctctgtaat gttgcggtag taaagaatct gggaatccga tgtgatatcc gaactaaaaa
     5101 ggattttcga cgtgccgaac ttcaaggcaa acaacaacct gccaaatagt ccgccgacct
     5161 ttaccccacc gtcgccctgg taggtagtgt agacgttgtc ttcaccactg gggtagttca
     5221 tctctttttg ccgggtcttc acgattacat agtcgtttgt tcgctcgccg aagtagattt
     5281 caggccgagt aacgtcaatg atgcctttag gcggtatgtc ccttaccaga aactcaggta
     5341 ttccctgagg agtcacgccg tgtatgctgc tcatgacgaa accgtaaccg tgagtgaaaa
     5401 cgaggtgctt gttgacccag cttctggcag tgcccgtaag gtagttcgaa tcgatctccc
     5461 gcacgctgag tgccatctgg gtagtttttc catcgacctg gtagcgatct atatcgatat
     5521 cgcccatttt gtagtagtag cggagttcct gaagctggga gaaggatttt tccagcgctc
     5581 tccaatccca cagcctgaca ttatccagca cttctgtatt atcgatccag tcgtcggagg
     5641 aaaaagccaa agctggttca aactgggcgg aaacgacttc atctaatcca tacgcaaaac
     5701 gggtgaaacc tatgttatgt tcgataaatc tccgctcttt gttcagctcg ttgggctcta
     5761 caactacctt ctgcagaatc ggcgcagcaa actgacctaa aatccccaca gcaacaagaa
     5821 ccaatagagt ccatacggtc cacttgtact ccctccgaaa gaggtctagg gcaaaaacaa
     5881 agaccgccaa gacgcagagg atctgcaaaa tcctgttgac cacaagtctg ccgtaaacgt
     5941 cgctgtagcc cgcaccgtaa acagcacctg tgggagagcg gagaagttca tagctgttga
     6001 tctgcagccc tagcaccgca agcgccacaa tgaggatgat gagtagaagc aaatgaaggc
     6061 gcgctcggtc gcctaggtac acccgacgtt tgtaaaaagt gatggacctt gttgctagat
     6121 agaaaactcc cgtaaacagc aaagtaaaca ctgcaactgc aaaggctgtg acatacaagc
     6181 cctggattaa aggcagcacg aagacgtaaa acgacacgtc catatgaaag atgggatctt
     6241 cgattccaaa aggattgcgc ccaagaaacc ttgctacagt ctgccagttg
//
LOCUS       DAOSDA010000037         6277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_7937_length_6277_cov_3.421890, whole genome shotgun sequence.
ACCESSION   DAOSDA010000037 DAOSDA010000000
VERSION     DAOSDA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7937_length_6277_cov_3.421890"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLW82_01525"
     CDS             complement(<1..774)
                     /locus_tag="PLW82_01525"
                     /inference="COORDINATES: protein motif:HMM:NF019931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HOJ45986.1"
                     /translation="MPKWKKLAKQLGFLEPTSCSLSQLESIAKHLDGRENYLDPHVLK
                     NFADFRFPEKKFPNGKFAISLALPYYHKPIFNSMGEYPSFSIYCQGKDYHFVIKYLLQ
                     EYLLDLKKAYPGHFFEGYCDTGPILDRAVALASGLGYLGFNTCIIHEARGSFVFLATV
                     LTDLPLDEGESLGPCQRCGACIKACPTSAMRKPYLVDNNICISALTQTKGFLTYEQME
                     MIKGHIFGCDICQLVCPHNLGIPSSSMITPFYKLQNPSFM"
     gene            812..1357
                     /locus_tag="PLW82_01530"
     CDS             812..1357
                     /locus_tag="PLW82_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOJ45987.1"
                     /translation="MNRQDYLALMKSQVKNANLQKHMLAAEAILRELAPRFGGDSEEW
                     GLAGLLHDIDYEQTADTPEQHGLLAAELLQEYNLPEHVIYAIKAHSGMLELKTDLDKA
                     LYAVDPLTGLIVAAALIHQDKKLASIDTQFVMNRFGVKSFARGANREQIKSCTSLGLD
                     LEEFISIGLEAMQKISDELGL"
     gene            1375..2313
                     /locus_tag="PLW82_01535"
     CDS             1375..2313
                     /locus_tag="PLW82_01535"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ45988.1"
                     /translation="MSELVIRRGELKDVAGIVDVHCSELDGTWYSFKGGVREEVEGPT
                     SLSLYERWLNGGSWMSPELCAVYINNLLLEGHIPLVAEQNGLIVGELELFFGWESDGN
                     LYAHIGVVQVHKDHQRKGIGALLIKEAINYARKRGAYYLTVRPREGLKDYYKNLGFGE
                     WLKTVVFTAEASPYVARITWEEDNTYFNKKKFSRFIMGQEQSSAQIWQIFHRNQFALP
                     EFTREPIHTGRASTDGQEILAIFYPHVYCGDMANVYAWSDTDIRAEHIWTLLTLAYHF
                     GYARVRSLLIEEHFERLKSKLPLEEMERQEIFRIEL"
     gene            2331..2999
                     /locus_tag="PLW82_01540"
     CDS             2331..2999
                     /locus_tag="PLW82_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOJ45989.1"
                     /translation="MRLSWQDLFEKLPFPVEEEYPWAILDGLDRFFARFTGYNAHTWL
                     LQNKDKAGTVEIRGNVYIADDAQIESGATIIGPAYIGPKSVIRHGAYLRGEVYLSEGV
                     IVGHATEVKRSLLLKNCSASHFNYVGDSVLGANVNLGAGVKISNFKNDGSVIFVGGSD
                     TGMTKVGAFIGNGSKLGCNAVTAPGTVIGKNCAVYPLAFVRGVVPDNSIVKLRQELDI
                     ISRS"
     gene            3046..4053
                     /locus_tag="PLW82_01545"
     CDS             3046..4053
                     /locus_tag="PLW82_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOJ45990.1"
                     /translation="MKQKKAITWILVAALVILAAWQIWLRFPNETYVVQQDSRLLMDT
                     VITITVVAKEGVAQETLDRGFEELRRVDTLTNRYIGDSELSKLNKERTLAVSKDLLHL
                     IEISEEYRHKSGNAYDARLGRLVDLWGFGSNRRSVPSEVQIAKVLPSGDIEINNSTVT
                     LPADVILDLGAVAKGYAIEQARNVMKRTAKAGLIVGGGSSVACWGEHPEKRPWRIGLR
                     HPRDTNKLLGVIELEDGWSLGTSGDYERYFIQDSKRYHHILDGRTGYPGQDVYATSVL
                     TKSSTLADILSTLLFVLGPEDGKAFLNENGFDEIGVIWVMAPGEIVTYEKGVKLPELS
                     F"
     gene            4105..6045
                     /locus_tag="PLW82_01550"
     CDS             4105..6045
                     /locus_tag="PLW82_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ45991.1"
                     /translation="MRLSKLGEKRRILLMLICFIAASVMLWVRLFLLQVVRAEELGTK
                     ALSQRLRPIPVDGARGDILDRNGILLAGSEELASVYAIPAEVPDKEKNAELLGRVLDL
                     EPEVVLERLQKRAAAVWLKKRITKEEELQIRELGIRGIGVVEAPKRVYPYGSLAAHVI
                     GISGIDNQGLEGLELVYDRYLTGERGSFMVERDASGREVPGATKVYEEPKKGKTLRLT
                     IDASFQAIAEKALAKRIEETQATSGLVIFVDVTNGEILAMASWPTFDPENYQDYPQAN
                     RRNIAVTDLYEPGSTFKIVTAAAAIEEGIATVSRRFFDPGYIVVSGWRVRCWRAGGHG
                     SQNLTEALENSCNIAFATLGMELGSKLINYAKKYGFGKKTGIDFPGEATGILYSPGPD
                     VPLVTWANIGFGQSISITPLQLVSFAAAVANGGVLYVPHLVKEIEGEGAFEPKGTRIL
                     DEATAEILREMMFSVVENGSGRTAQMEGYTAGGKTGTAQMVVDGRYSHTSVIASFVGI
                     APAENPAIAGIIAIHEPRTMQYGGQVAGPVFAEIVAEMLPALGIEPVPEELVTNPWGG
                     PNKRVAKAIVPVPDVIGKSVLEAQNIMRSSSLMPEVVGQGTKVLDQEPKLGQSLKEGS
                     KVYLFVDTETILEGPPIVDAAN"
     gene            6117..>6277
                     /locus_tag="PLW82_01555"
     CDS             6117..>6277
                     /locus_tag="PLW82_01555"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016740849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOJ45992.1"
                     /translation="MQEERFAKVGLTFDDVLLIPAESYVLPRDVDLKTRVTRNISLNI
                     PLLSAGMDTV"
BASE COUNT         1679 a         1328 c         1710 g         1560 t
ORIGIN      
        1 cataaaggat ggattttgca gcttgtaaaa aggtgttatc atagaactag aaggaatgcc
       61 taggttgtgg ggacagacca gctggcagat gtcgcagccg aagatgtgtc ctttaatcat
      121 ttccatctgt tcataggtga gaaacccttt ggtctgggtc agggcggaga tgcagatgtt
      181 gttgtctacc agatacggct ttctcatagc actagtggga caagccttga tacacgcacc
      241 gcatctttga cacggcccaa gagactcccc ttcgtccaga ggcagatctg tcagtaccgt
      301 tgcaaggaat acaaatgatc cgcgcgcttc atgaatgatg caggtgttga agccgagata
      361 cccgagccca gatgctagag ctaccgctct atcgaggatc gggccagtgt cacagtaacc
      421 ctcaaagaaa tgccccggat aggccttttt cagatccaga aggtattcct gcagtagata
      481 cttaatgaca aagtggtagt cttttccttg gcagtatatg gaaaaagatg gatattcccc
      541 catagagttg aagattggtt tatgatagta gggtagcgca agggatattg cgaacttacc
      601 attggggaac tttttttccg gaaagcgaaa atcagcgaag tttttaagaa catggggatc
      661 tagatagttt tctctgccgt ctaaatgctt ggcgatggat tcaagctggc ttaacgagca
      721 gcttgtgggt tccagaaaac caagttgttt ggcaagtttt ttccatttcg gcacagtatc
      781 ccctcctagt ttctacaggg agtgaatgaa gttgaatcgc caggattact tagcgttgat
      841 gaaaagccaa gttaaaaacg ccaatttgca aaagcacatg ctagctgcag aagcaattct
      901 cagagaactc gctcctcgtt ttggcggtga cagcgaagag tggggtcttg ccggcttgct
      961 ccacgatatc gactacgagc agacggcgga cactccagag cagcacggct tgttggctgc
     1021 cgagcttcta caggaataca acctgccaga acatgtcatc tatgccatca aagcccattc
     1081 gggtatgttg gagctaaaaa ccgacctgga taaagccctg tatgcagtcg atccccttac
     1141 cgggcttatc gttgcagcgg ctttgattca tcaagacaag aagcttgctt caatagacac
     1201 gcagtttgtt atgaaccgtt ttggggtgaa atcatttgcg agaggtgcaa acagggagca
     1261 gattaagtcg tgcacttctc taggtcttga tctggaggag tttatctcta ttggtcttga
     1321 agcaatgcag aagatctctg acgagctcgg actatagata tggtaggtgg acatatgagc
     1381 gaattagtga ttcgcagagg cgagctcaag gatgttgccg ggattgttga tgtccactgt
     1441 tcagagttgg acggcacctg gtacagcttt aaaggcggag tgcgagaaga ggtcgaggga
     1501 ccgacttctc tttctctgta tgagcgctgg ctgaacggcg gctcgtggat gagtcccgaa
     1561 ctctgcgcgg tatatattaa caatttacta ctagaaggtc atataccttt agtggccgag
     1621 caaaacgggt tgatcgttgg agaactggag ctgtttttcg gttgggaaag cgacggtaat
     1681 ctctatgcgc atatcggggt tgttcaagtt cataaagacc accagcgcaa gggtattggg
     1741 gctttgctca tcaaagaggc gatcaactac gcaagaaaac gcggtgccta ctacctgacg
     1801 gtaaggccca gggaaggttt aaaagattat tacaaaaacc tcggttttgg agagtggctg
     1861 aagacagttg tctttaccgc tgaggccagt ccatacgttg ccaggatcac gtgggaagaa
     1921 gataacacct acttcaacaa gaaaaagttc tcgcgcttca ttatgggaca ggagcagtcg
     1981 tctgcacaga tctggcagat tttccaccgt aatcagtttg ccctgcctga gtttaccaga
     2041 gaaccgattc acactgggag agcgagcaca gacggccaag agatattggc tatattttat
     2101 ccgcacgttt attgtggaga tatggccaat gtttatgctt ggtccgatac ggacattaga
     2161 gcggaacata tctggactct tctcacattg gcctatcatt ttgggtatgc tagagtaaga
     2221 agcctgctta ttgaagagca ttttgaaagg ttgaagagca aactcccact cgaggagatg
     2281 gaacggcagg aaatctttcg tatcgaactg taaatggagg ttttactttc atgcggcttt
     2341 cttggcagga cttgtttgaa aagctgcctt ttcctgtaga agaagagtat ccctgggcta
     2401 ttctagacgg actggatcgc ttttttgcaa ggtttaccgg ctacaatgca cacacttggc
     2461 tgctgcaaaa caaggacaag gcaggcacgg tagagattag aggcaatgtt tacattgcag
     2521 atgatgctca aattgagagc ggtgcaacga ttatcggtcc ggcatatatt ggacctaaat
     2581 ctgttattcg ccatggggca tacttgagag gcgaagtata cctcagcgaa ggcgttatcg
     2641 tgggacatgc cacagaggtc aaacgctccc tccttcttaa gaattgcagc gcttcacact
     2701 tcaattacgt tggcgatagc gttttgggtg ccaacgtcaa cctcggtgcg ggtgtaaaga
     2761 tctccaactt caaaaacgat gggtctgtga tttttgtcgg cggtagtgat acgggcatga
     2821 ccaaggttgg tgcttttatt ggcaatggca gcaagctggg ttgcaacgca gtaactgctc
     2881 caggtacggt tattggtaag aactgtgctg tctatccttt ggcttttgtt cgaggtgttg
     2941 ttccggataa ttctatagtg aagttaaggc aagaacttga tataatcagt aggtcataaa
     3001 tgcgcctaat ctgaaaggag ttgaacacct ctaaggtagt cgacaatgaa gcaaaaaaaa
     3061 gctattactt ggattttagt tgcagcactg gtaatacttg ctgcatggca gatttggctg
     3121 cgttttccta acgaaacata cgtggtacag caggacagca ggctgctcat ggataccgtc
     3181 attactatta cagttgtagc taaagaaggt gtcgcacaag aaactttaga cagaggtttt
     3241 gaggagctgc gccgggttga taccctcacc aatcgctaca ttggtgatag cgagctttcc
     3301 aaactgaaca aggaaaggac gcttgcggtc agcaaggatc ttttgcacct aattgagatt
     3361 tcggaagagt accgtcataa aagcggcaat gcgtatgatg ctcgtcttgg tcgcttggtt
     3421 gacctttggg gttttggttc gaacaggaga agcgtacctt ccgaggtgca gatcgccaaa
     3481 gttctgccgt ctggagacat tgagattaac aacagcaccg taactctccc tgctgacgtc
     3541 attttggact tgggagcagt agctaagggt tatgccatag aacaggcaag aaacgttatg
     3601 aagcgaactg caaaagccgg cctgattgta ggagggggaa gttctgttgc ctgttggggt
     3661 gaacatcccg aaaagaggcc ttggcgtata ggcctgcggc atccaaggga tactaataag
     3721 ttgcttggag taattgagct tgaggacggc tggtctctcg gtacatcagg tgactacgaa
     3781 aggtacttca ttcaggattc caaaaggtat caccacatcc ttgacggcag aacgggatat
     3841 cccggccaag acgtctatgc cacaagcgtg ctaaccaaaa gcagcacgct ggcagatatc
     3901 ctttcaacgc tgttgtttgt cttaggtccc gaagatggca aagcgttctt gaacgaaaat
     3961 ggttttgacg aaatcggcgt gatctgggtt atggctccgg gagagatcgt cacatacgaa
     4021 aaaggcgtga aactgccaga gctgagtttt taatatcata cgagaaagct gataaccgta
     4081 cacttctaca ggaggtgtac ggttatgcgt ttgtctaagc ttggagaaaa aagaagaatc
     4141 ctcctcatgc tcatctgctt catcgccgct agtgtaatgc tttgggtgcg gctgtttctt
     4201 ctgcaggtag taagagctga ggagttaggc accaaagctc tttcacaaag gcttcgaccg
     4261 atccccgtcg atggagcaag aggcgacatc ttggatagaa atggtattct tcttgcaggt
     4321 tcggaggaac tggcatcggt atacgcaatt cctgctgaag ttccggacaa agaaaaaaac
     4381 gcggagctat tgggaagagt cttggactta gagccagagg tagttttgga gagactccaa
     4441 aaaagagctg cagcagtttg gctgaaaaaa cgcatcacca aagaagaaga gttgcaaata
     4501 agagagttgg gtattagagg cattggtgtt gtagaggcac ctaagcgtgt gtacccctac
     4561 gggagtcttg cggcgcacgt catcggcatc tccggtatag acaatcaagg tctcgaaggc
     4621 cttgagctgg tgtacgatcg gtatctcacc ggagagcggg gcagcttcat ggtggaaaga
     4681 gacgccagtg ggagagaagt gcctggggca accaaagtct acgaagagcc gaaaaaggga
     4741 aaaaccctac gcttaaccat cgacgcttct tttcaagcaa tcgctgaaaa agctcttgcc
     4801 aagaggattg aagagaccca agccacatcg ggccttgtga tcttcgtaga tgttacaaac
     4861 ggagagattc tagccatggc ttcatggcct actttcgatc ctgaaaacta ccaggattat
     4921 ccccaagcta acaggcgaaa tattgccgtc acagaccttt acgaaccggg atctacgttt
     4981 aagatcgtca ccgccgcggc tgccatagaa gaaggcatcg cgacggtaag ccggaggttt
     5041 tttgatcccg ggtatattgt ggtttccggg tggagagtta ggtgctggag ggcaggagga
     5101 catggtagcc aaaacctaac ggaagcattg gaaaacagct gcaacattgc ctttgccaca
     5161 ttaggtatgg agcttggaag caagctgatc aactatgcta aaaagtacgg ttttggtaag
     5221 aagactggta ttgattttcc tggagaagct actggcattc tctacagccc cggacctgac
     5281 gttcctttag tgacttgggc gaacatcggc tttggtcaat cgatcagcat cacgcctctc
     5341 cagcttgtga gctttgcagc agcggtcgcc aacggtggtg ttctgtacgt tccccatctg
     5401 gtcaaggaaa tcgagggcga aggggctttc gaacccaagg gaaccagaat cttggacgaa
     5461 gccacagcgg aaatactaag agaaatgatg ttttccgttg tggaaaacgg gtccggcagg
     5521 acggcgcaga tggaaggcta cactgcagga ggcaagaccg gtactgctca gatggtggta
     5581 gatggccgtt acagccacac ctcagtgata gcgtcgtttg tgggcattgc accagccgaa
     5641 aatcctgcaa tcgcagggat aatcgccatc catgagccga gaactatgca gtacggaggg
     5701 caggttgcag gaccggtctt tgctgagatt gtcgcggaga tgctgcctgc actgggtatc
     5761 gagccggtac ccgaggagct ggtcaccaat ccgtggggag gaccaaacaa aagggtagcg
     5821 aaagcaattg ttccggtacc ggatgtaatt ggcaaaagcg ttttggaagc gcaaaacatc
     5881 atgcgcagca gttccttgat gccggaagtt gtcggacagg ggacgaaagt tttggaccag
     5941 gaacctaagc taggtcaatc tcttaaggaa ggatctaaag tctatctttt tgttgataca
     6001 gagacgattt tggagggtcc tcctatagtt gacgctgcaa actaggtctg atataataac
     6061 cacaatttac aggtgcagtt acgattttcg cacaataaaa aacgaggtgg aattgcttgc
     6121 aagaggaacg ttttgccaaa gttggattga cgtttgacga tgttttgctg atacctgcag
     6181 agagctacgt tctgcccaga gatgttgatc tgaagacgag ggttactcgc aacatctcgc
     6241 tgaacatccc actacttagt gcaggtatgg atacggt
//
LOCUS       DAOSDA010000038         6223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_8018_length_6223_cov_3.207361, whole genome shotgun sequence.
ACCESSION   DAOSDA010000038 DAOSDA010000000
VERSION     DAOSDA010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8018_length_6223_cov_3.207361"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /gene="rplB"
                     /locus_tag="PLW82_01560"
     CDS             <1..309
                     /gene="rplB"
                     /locus_tag="PLW82_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777325.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOJ45993.1"
                     /translation="TLRLPSGEMRMILETCRATVGQVGNVEHENMKIGKAGRSRWFGK
                     RPHVRGVAMNPVDHPHGGGEGRAPIGHSSPLTPWGKPTLGYRTRKKKPSDRLIVRRRR
                     "
     gene            357..641
                     /gene="rpsS"
                     /locus_tag="PLW82_01565"
     CDS             357..641
                     /gene="rpsS"
                     /locus_tag="PLW82_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOJ45994.1"
                     /translation="MSRERKQPYICKKLLKRVEEMNKTGEKKVIKTWSRSSTIYPELV
                     GHTFAVHDGRKHVPVYVTEDMVGHKLGEFAPTRTFRGHSGTAERASALKK"
     gene            660..1001
                     /gene="rplV"
                     /locus_tag="PLW82_01570"
     CDS             660..1001
                     /gene="rplV"
                     /locus_tag="PLW82_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOJ45995.1"
                     /translation="MEAKATARMIRMSPRKVRQVIDAIRGKSLQEAQQILKVTPRRAT
                     YYIGKVLNSAAANAENNYNMDRDLLYVKKAYVDEGPTMKRYRFHSRGQVDTIFKRTCH
                     ITVVLSEKKEG"
     gene            1006..1641
                     /gene="rpsC"
                     /locus_tag="PLW82_01575"
     CDS             1006..1641
                     /gene="rpsC"
                     /locus_tag="PLW82_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOJ45996.1"
                     /translation="MGQKTHPLGMRLGIIKDWDARWYAEKDYAALVQEDLMIRKTIKK
                     RFMSAGIARVEIERAANMVKITIHTAKPGMVIGKGGALVDEFRKELEKKTGRQISINI
                     VEVKKPDLNSQLVAENIASQLERRISFRRAMRQAKQRAMKAGALGIKIAVSGRLGGAD
                     IARTEWTPEGSVPLQTLRADIDYGFAEAYTTYGMIGVKVWIYKGEVLPEKK"
     gene            1666..2097
                     /gene="rplP"
                     /locus_tag="PLW82_01580"
     CDS             1666..2097
                     /gene="rplP"
                     /locus_tag="PLW82_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOJ45997.1"
                     /translation="MLIPKRVKYRKVQRGRMKGKAIRGSQVQSGEYGLQALEPAWITS
                     NQIEAARVAMTRSIKRGGKVWVKIFPDKPVTKKPAEVRMGSGKGSPEYWVAVVRPGRV
                     MFEIAGVPEDVAREALRLAAHKLPIKTKFVSREKAGDANES"
     gene            2087..2287
                     /gene="rpmC"
                     /locus_tag="PLW82_01585"
     CDS             2087..2287
                     /gene="rpmC"
                     /locus_tag="PLW82_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOJ45998.1"
                     /translation="MKASEIRDLNDAELRKQLGDLKEELFNLRFQLATGQLDNVKRIS
                     EVRKSIARVKTVIKERELQAKK"
     gene            2320..2589
                     /gene="rpsQ"
                     /locus_tag="PLW82_01590"
     CDS             2320..2589
                     /gene="rpsQ"
                     /locus_tag="PLW82_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOJ45999.1"
                     /translation="MASEATREKNPKTRIGEVVSDKMDKTVVVEVVRLYSHPLYSKRV
                     KTTKRYKAHDENNECKVGDKVRIEECRPLSRDKHWKVIEIVERAK"
     gene            2623..2991
                     /gene="rplN"
                     /locus_tag="PLW82_01595"
     CDS             2623..2991
                     /gene="rplN"
                     /locus_tag="PLW82_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOJ46000.1"
                     /translation="MIQQESRLTVADNSGAREILCIRVVGGTGRRYASVGDEIIATVK
                     EATPGGNVKKGEIVRAVIVRTRERVGRPDGSYIRFDDNAAVIVDTQKNPRGTRIFGPV
                     ARELREKEYMRIISLAPEVL"
     gene            3044..3367
                     /gene="rplX"
                     /locus_tag="PLW82_01600"
     CDS             3044..3367
                     /gene="rplX"
                     /locus_tag="PLW82_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009753881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOJ46001.1"
                     /translation="MNMQKIHVKKGDTVVVLSGNDKGKSGKVLRVDHKKGRVVVENLN
                     LVKKHQRPDRENPKGGIIEIPAPMNSSKVMVVCPNCGQATRIKKELQGDKKVRICKKC
                     DKAID"
     gene            3399..3941
                     /gene="rplE"
                     /locus_tag="PLW82_01605"
     CDS             3399..3941
                     /gene="rplE"
                     /locus_tag="PLW82_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOJ46002.1"
                     /translation="MPRLKDFYQTEVSKAMMEKFGYKSTMEVPKLVKVVVNMGVGEAK
                     EDPKALDAALKELGAITGQKPVITRAKKSVAAFKIRQGMAIGCKVTLRGDRMYDFLDR
                     LLSIGLPRIRDFQGISPKGFDGRGNFTLGIKEQIIFPEVSLDEVSSIRGMDISVVTTA
                     QTDEEAFELLKLLGMPFKSA"
     gene            3986..4171
                     /locus_tag="PLW82_01610"
     CDS             3986..4171
                     /locus_tag="PLW82_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOJ46003.1"
                     /translation="MAKKSMRLKAAKPNKFAVRQHNRCNICGRPRAYMRKFGMCRICF
                     RKLALEGKLPGVRKASW"
     gene            4223..4621
                     /gene="rpsH"
                     /locus_tag="PLW82_01615"
     CDS             4223..4621
                     /gene="rpsH"
                     /locus_tag="PLW82_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOJ46004.1"
                     /translation="MITTDPVADMLTRIRNAGAVYHEKVDIPTSKLKLEIARILKEEG
                     YIKDYRLITENNHQMIRVFLKYGDKKRPVIAGLKRISKPGLRVYAGANEIPKVLGGLG
                     TAIISTSKGVMTDRDARKLNVGGEVLCFIW"
     gene            4667..5209
                     /gene="rplF"
                     /locus_tag="PLW82_01620"
     CDS             4667..5209
                     /gene="rplF"
                     /locus_tag="PLW82_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOJ46005.1"
                     /translation="MSRIGLKAIKIPSGVEVKLDGSVLTVKGPKGTLTYEITEGIDTV
                     IEDGTIHFARQNESLKAVHGLTRSLVNNMIVGVTQGFSKTLIIQGVGFRATKDGKNLV
                     LNVGYSHPVVMEPYEGIEIEIPEPTKVVVKGIDKQKVGEFAAKIRKVREPSVYRTERG
                     NKGIIYEGEVVRSKAGKTGA"
     gene            5274..5636
                     /gene="rplR"
                     /locus_tag="PLW82_01625"
     CDS             5274..5636
                     /gene="rplR"
                     /locus_tag="PLW82_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOJ46006.1"
                     /translation="MALTRREARMRRHMRSRNRISGTPERPRLAVFRSNRHIYAQVID
                     DVSGTTLVAASTLSPELRDVLDYTGNIDAAQKVGELIAKKAQEKGISKVVFDRGGNLF
                     HGRVKALAEGAREAGLEF"
     gene            5663..6163
                     /gene="rpsE"
                     /locus_tag="PLW82_01630"
     CDS             5663..6163
                     /gene="rpsE"
                     /locus_tag="PLW82_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009791312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOJ46007.1"
                     /translation="MAQIDPNKLELKERVVSINPVSKTVKGGKNRSFRALVVVGDGQR
                     YVGVGLGKAREVTDAIRKGVDEAKKNLIEVPAVNGTIPHPITQRYGGAQVLLRPASEG
                     TGVIAGGAVRAVLELGGIQNVLSKSLGSPNAINVAMATLEALKNLRTREDIARLRGID
                     VERLAE"
BASE COUNT         1905 a         1302 c         1670 g         1346 t
ORIGIN      
        1 actttgcgtc tgccttcagg tgagatgaga atgatactgg agacctgtcg tgccacagta
       61 ggtcaagttg gcaacgtgga acacgaaaac atgaagatcg gtaaagcagg tagaagccgc
      121 tggttcggta aacgtccaca cgttcgcggc gttgctatga acccagtcga tcacccacat
      181 ggtggtggcg aaggccgtgc tcctatcggt cattctagtc cccttactcc ttggggcaaa
      241 ccgacactcg gttaccgcac caggaagaag aagccttctg atcgcttaat cgtcagaaga
      301 agacgctagg gtgcgttgtt atgtacttca gatattgaag gggggtcgtg gcaagcatgt
      361 caagagaaag aaaacagcct tatatttgta agaagctgtt gaagcgggtt gaagaaatga
      421 ataagactgg tgagaagaaa gtcattaaga cttggtctcg cagttccaca atatatccag
      481 aattagttgg ccacacattt gcagtgcacg acggtcgaaa gcatgtccct gtttatgtta
      541 ctgaagatat ggtagggcat aagttgggcg agttcgcgcc aacacggact ttccgggggc
      601 atagcggcac agccgaacgc gcgtcggccc tcaaaaaata gcgagagggg gatagcttta
      661 tggaagctaa agctactgct cgcatgattc ggatgtcgcc aagaaaggta cgtcaagtaa
      721 ttgatgcaat ccgtggtaag agtcttcaag aagctcaaca gattttgaag gttactccga
      781 gaagggctac ttactacatt ggcaaagtcc tgaattctgc ggcggcaaat gcagagaata
      841 attacaacat ggatcgtgac ttgttgtatg taaagaaggc ctatgtggat gaaggtccta
      901 caatgaagag atacagattc cactccagag gccaggtaga tacaattttc aagagaacat
      961 gccacattac agtggtacta agcgaaaaga aggagggatg aacagatggg tcaaaaaacg
     1021 catcccttag gtatgagatt gggaatcatt aaggactggg atgccaggtg gtatgctgaa
     1081 aaggactacg ctgctctcgt tcaggaagac ttgatgatta gaaagaccat caagaagaga
     1141 ttcatgagcg cgggaatagc aagagtcgag attgaaagag cagccaacat ggtaaagatc
     1201 actatccaca ccgcaaagcc aggtatggtt atcggcaaag gcggtgcgct ggtagatgag
     1261 ttcagaaaag aactcgaaaa gaagactggc agacagatct ccataaacat cgtggaagta
     1321 aagaaacctg acttgaactc gcagcttgtg gctgagaaca ttgcaagtca gctggaaaga
     1381 agaatctcct tccgccgtgc catgcgtcag gctaagcaga gagcgatgaa ggctggagcg
     1441 ttgggtatca aaattgcagt ttctggtcgt ttaggcggag ccgacatcgc cagaacagaa
     1501 tggactcctg aaggttcagt tcctctgcag acccttcgtg ccgacatcga ttacggattt
     1561 gccgaagcct acaccactta cggaatgatc ggcgtcaagg tatggatata caaaggagaa
     1621 gtccttcccg aaaaaaaata aggagtcgca aggaggcgag aggcaatgct tataccaaag
     1681 cgtgttaagt accgtaaagt acagcgcggc cgtatgaagg ggaaagccat ccgcggctca
     1741 caggtacaat ctggtgagta cggtttgcaa gcattagagc cagcatggat cacttcaaat
     1801 cagattgaag ctgcccgtgt tgccatgaca agatcgatca aacgcggcgg taaagtctgg
     1861 gttaagatat tccccgacaa acctgttacc aagaagcctg ctgaagttcg catgggtagc
     1921 ggtaaaggat ccccagagta ctgggtagcc gttgtgcgtc ctggtcgtgt aatgttcgaa
     1981 attgcgggtg ttcccgaaga cgtcgctaga gaagcattga gactggcagc tcataaactg
     2041 ccaattaaga caaagttcgt atctagagag aaggcaggtg acgcaaatga aagctagtga
     2101 aatcagagat cttaatgatg ctgaactcag aaagcagctt ggtgatctca aagaagagct
     2161 attcaacttg cgtttccagc tggcaacagg tcaacttgac aatgtcaaga gaatttcaga
     2221 agtacgtaaa tctattgcca gagtaaaaac agtgattaaa gagcgagagt tacaagctaa
     2281 gaaataacgc cccagataga gagaaggagg ggagttctat tggctagtga agctaccaga
     2341 gaaaagaatc caaaaaccag aatcggcgaa gtggtaagcg acaaaatgga taagaccgtt
     2401 gtagttgagg ttgtaagact ttactcacat cctctctaca gtaagcgcgt aaagaccaca
     2461 aagcgctata aagcacacga cgaaaataac gagtgcaaag ttggcgataa ggtgcgcatt
     2521 gaagagtgcc gcccgctcag cagagataag cactggaagg ttattgagat cgtagagaga
     2581 gcgaagtaat agcaacttgt atgtaagaga ggaggggtag agatgattca acaagagtcg
     2641 aggttgaccg tggctgacaa ctcaggagca cgcgagattc tctgcatcag agttgtaggc
     2701 ggtactggac gcagatacgc aagcgtaggc gatgagatca ttgccaccgt caaagaagca
     2761 actccaggcg gaaacgtaaa gaagggcgag atcgtacgcg ctgttatcgt gagaactcgt
     2821 gagcgtgtcg gaagacctga tggcagctat atccgttttg acgacaatgc agcagtaatt
     2881 gtcgataccc agaagaatcc tcgcggtact cgtatctttg gcccagttgc cagagaactc
     2941 cgtgagaagg aatacatgcg tatcatctcc ctagccccgg aggtactcta aggtccagca
     3001 ccggggacaa taaagctcac taaagggggg aacaagcaca gccatgaaca tgcaaaagat
     3061 tcacgttaaa aaaggcgata ctgttgtcgt actgtccggt aatgataaag gtaagagcgg
     3121 caaagtactt cgcgtagatc ataaaaaagg tagagttgtt gttgaaaacc tcaacctggt
     3181 aaagaagcat caacgtcccg atcgtgaaaa tccaaagggc ggaattattg agattccagc
     3241 tccaatgaac agtagtaaag ttatggttgt ctgccccaac tgtggtcagg ccacccgtat
     3301 caagaaagaa ctccaaggtg ataaaaaagt acgtatctgc aagaagtgcg ataaagcaat
     3361 cgactgagtt tgcacttggg aaaggaggtt gaactcccat gccacgtctt aaagatttct
     3421 atcaaacaga agtatccaag gccatgatgg aaaagttcgg ctacaagagc acaatggaag
     3481 taccaaaact ggtcaaagtt gtggtcaaca tgggcgtagg tgaagccaag gaagatccaa
     3541 aggctttgga tgctgcactg aaagaactag gcgctatcac aggtcagaaa cctgttatca
     3601 ctagagcgaa gaagtccgtt gccgctttca aaatccgcca gggcatggca ataggctgca
     3661 aagttactct aagaggagat cgcatgtacg atttcttaga cagactgctt tctattggtc
     3721 tgccccgcat tcgtgacttc caaggcatct cacccaaagg atttgacggt cgcggtaact
     3781 tcacgttagg aataaaagaa cagatcattt tcccagaggt cagtttggac gaagtaagct
     3841 ccatcagagg catggatatt tccgttgtca caacagctca gactgacgaa gaggcctttg
     3901 aactgttaaa acttctaggt atgccattta agagcgcgta actgtggctg cctaggccac
     3961 tactactcat agaggaggga agagcgtggc caaaaaatca atgagactta aagctgcaaa
     4021 acccaacaag tttgcagtaa gacagcacaa tcgctgcaac atctgcgggc gaccacgtgc
     4081 atatatgcgt aaattcggaa tgtgccgcat ctgcttccgc aagctagctc tagagggcaa
     4141 attgccagga gttcgcaagg ctagctggtg acatgtggca taatccttgc tgaatttttg
     4201 ttaatggaag ggggatgcta ggatgatcac gactgatccc gtagctgata tgctgacgag
     4261 aatccggaat gctggcgctg tttaccacga aaaagttgat attccaacct ccaaactgaa
     4321 gctggaaatt gcccggatcc tcaaggaaga gggctatatc aaggactatc gacttataac
     4381 agaaaacaat catcaaatga ttagggtctt tctcaagtac ggagataaga aaagaccagt
     4441 catcgcagga ctgaagagaa taagcaagcc aggtctgcgc gtttacgccg gtgccaacga
     4501 aatccccaag gttcttggtg gactcggaac tgctattatc tcgacttcaa aaggcgtaat
     4561 gaccgataga gacgccagaa aactgaatgt aggtggagaa gtactctgct ttatttggta
     4621 gaaacgcaga aacggttgaa ttctgctgca aggaggtgtg gttgctttgt cacgcatcgg
     4681 actaaaggca attaagattc ccagcggtgt cgaagtgaag cttgacggtt cggttctgac
     4741 cgtaaaggga ccgaagggca cgctcactta cgagataact gagggtattg atacagtaat
     4801 tgaggacgga acaattcatt ttgctcgtca aaacgagagt ctgaaggcag ttcacggttt
     4861 gacaagatcc ctggtaaaca acatgatcgt aggcgttacc caaggattct ccaaaacgtt
     4921 aatcatccaa ggtgtcggtt ttagggccac caaagatggt aagaacctgg tactcaacgt
     4981 aggttattcc cacccagtgg tgatggagcc ctatgaaggt atcgaaattg aaatccccga
     5041 gccaaccaag gttgtggtta agggtatcga caagcagaag gttggagagt tcgccgccaa
     5101 aatcagaaag gttcgcgagc cttctgttta cagaactgaa cgcggcaata aaggtattat
     5161 ttacgaaggc gaagtcgtgc ggagcaaagc cggcaaaacc ggtgcttaat agaccggctg
     5221 cctaggcaaa atgagaaaaa ttatctctac cgtacgaaag gagtgagtgt ctcgtggctt
     5281 taactcgtag agaggctaga atgcgtcgcc atatgcgttc tagaaataga atatctggta
     5341 ctcctgaaag accaagactt gcggttttcc gcagcaatag gcatatctac gcacaagtaa
     5401 tcgatgatgt ttctggcact actttggtag cagcttcgac tttgagccca gaactcagag
     5461 atgtgctaga ctatacaggc aacatcgacg ctgcccagaa ggttggcgaa ctgatagcca
     5521 agaaagccca ggaaaaaggc atcagcaaag tggtctttga ccgcggcggc aacctgttcc
     5581 acggcagggt aaaagccctg gccgaaggtg cgagagaagc gggtctcgaa ttttaagaac
     5641 acgggcaaag gagggaaaga atttggcaca aatagatccc aacaaactgg agctaaaaga
     5701 acgtgttgtt agcatcaacc cagtttctaa gactgttaaa ggtggtaaga accgcagctt
     5761 cagagccctg gtagtcgttg gtgacggaca gaggtatgta ggcgttggcc tcggtaaagc
     5821 cagagaagtc accgatgcga ttcgcaaggg tgtggacgaa gccaagaaga atctgatcga
     5881 agttccagcc gtcaacggta ctatacctca tccgatcacc cagcgttacg gaggtgcgca
     5941 agttctgctc agacctgcat ctgaaggtac cggagttatt gccggtggag cagttcgcgc
     6001 cgtattagag ctcggtggta ttcagaacgt gctgagcaag tctctcggct cccccaacgc
     6061 aatcaacgtt gcgatggcaa ctttggaggc cttgaagaat ctcagaacga gagaagatat
     6121 cgctcgctta agaggcatag acgtagaaag acttgcggaa taaatataga tgctaaagtt
     6181 tgaagcaagg aggtggcaat atgcaacttc atgagctaag ccc
//
LOCUS       DAOSDA010000039         6168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_8103_length_6168_cov_3.165712, whole genome shotgun sequence.
ACCESSION   DAOSDA010000039 DAOSDA010000000
VERSION     DAOSDA010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8103_length_6168_cov_3.165712"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /locus_tag="PLW82_01635"
     CDS             <1..1665
                     /locus_tag="PLW82_01635"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOJ46008.1"
                     /translation="VSIDVLVTIAVIGAFAIRNFEESAVVTFLFLFGAYLEQRTLNKT
                     RSAIKELTEMAPEVALKLMEDGEFAEVDVDDVDIGDVLLVKTGAKVPVDGTVVAGEGS
                     VNQASITGESIPVLKEQGSKVFAGTILDNGTLQIVAERVGEDTTFGRIIELVEEAQDS
                     KSEAERFIDRFAKYYTPAVLVLAFIVWLFSKDVELAITILVLGCPGALVIGVPVSHVA
                     GIGNGARHGILLKGSEVISDFSRVDTFVFDKTGTLTVGEPEVAYKEYYGEEVDRALSY
                     LASIERESDHPLAKAVLEEIGETQLYPVENTQVVKGGGIIADVDGHSVIVGNMSLMER
                     EQVELDSKMRLRVAELEQSGHSLVLTAVDRQLKVVMGVRDQVREGVKEDLRKLKKLGV
                     KHLVMLSGDNQGTVDAVSRELGLTEAHGNLLPEDKSAYVKKLMESGRIVAFVGDGVND
                     SPSLAYANIGIAMGSGTDVAIETSDVVLMNSDFSLLPVALGLAKAISRNMKQNIVIAV
                     GVVLVLLFSLIFGSWMNMSIGMLVHEGSILLVILNGMRLLRYGLKR"
     gene            1746..1973
                     /locus_tag="PLW82_01640"
     CDS             1746..1973
                     /locus_tag="PLW82_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOJ46009.1"
                     /translation="MKTATIQLETLTCPSCMQKIEGAIKNLDGVAKDTVNVMFNSSKV
                     KLEFDEDKIALEDITNAIIKVGYEVKSAKSK"
     gene            2217..2904
                     /locus_tag="PLW82_01645"
                     /pseudo
     CDS             2217..2904
                     /locus_tag="PLW82_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
     gene            3173..4978
                     /gene="ftsH"
                     /locus_tag="PLW82_01650"
     CDS             3173..4978
                     /gene="ftsH"
                     /locus_tag="PLW82_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOJ46010.1"
                     /translation="MDKLRKPRKSLLFYYGIFILILAIFNFMVLPWILERQIIEVDYR
                     TFIKMTEENQIGQVQIQQNRILFTDKENTMVFKTGLMDDPNLVERLYKSDAVFSGQIV
                     EQMSPIMSILLSWVLPMVIIFVVGQYFAKKIMDKTGAGNALTFGKANAKVYVQSEEGI
                     KFSDVAGADEAKENLAEIVDYLHNPQKYREIGASMPKGVLLVGPPGTGKTMLAKAVAG
                     ESNVPFFSIAGSEFVEMFVGLGASKVRDLFKQAKEKAPCIVFIDEIDAIGKKRDGSVG
                     GNDEREQTLNQLLTEMDGFEENTGVIILAATNRPEALDPALTRPGRFDRRIPVELPDL
                     KGREAILKVHARKIKLAEGVDFEKVARMASGASGAELANIINEAALRAVRENRMLVTQ
                     DDLEASIEVVIAGYEKKNAILSDKEKWAVAYHEIGHALVAAKQTNSPPVQKITIIPRT
                     SGALGYTMQVDEGDKYLMTKEDIESRIATFTGGRAAEEVALGTMTTGASNDIEQATRL
                     ARSMITRFGMSEEFGMVAMETVTSQYLGGDTSLVCSAETQAKIDRATADIIRRQYEKA
                     KRILEENKEKLDELAKYLYEKETITGAEFMEILGS"
     gene            5054..5773
                     /locus_tag="PLW82_01655"
     CDS             5054..5773
                     /locus_tag="PLW82_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HOJ46011.1"
                     /translation="MLTLWLFLPLGAIFVFIGLAVHVFKWYSMISGYNTMSKEQKANV
                     DVEGLSKAVGIFCYVNGGLFLLTGILSALGVKVSVFIPFGVLLVSTPHFVSSVRKYDK
                     NTETQKAAKGLNWTMMITLVLVAVLFFFATRPTNINVGDEGIQITGLYGGTYTWESIE
                     SVSLEDTLPKIEMRTNGSKVGSHLKGYFRTTEFGQVKMALDAAKPPFIYMKTDGKMFI
                     FNAGDSEKTRQLYEEIADRLQ"
     gene            complement(5965..>6168)
                     /locus_tag="PLW82_01660"
     CDS             complement(5965..>6168)
                     /locus_tag="PLW82_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46012.1"
                     /translation="IRACMDSLEKAGKIQNTFINPFYRKPPWEIKVEREGKKIGVINP
                     FREEGLDRLHPGIRAGEQKESKF"
BASE COUNT         1691 a         1336 c         1648 g         1493 t
ORIGIN      
        1 gtaagtattg acgttctggt aaccatcgcg gttataggag cgtttgctat aagaaacttt
       61 gaagaatctg ccgtagtcac atttctcttc ctctttggtg cttacttaga gcagcgcact
      121 ttgaacaaaa ctcgttctgc tatcaaagag cttacagaaa tggcaccaga agtagcactc
      181 aagttgatgg aagacggcga gtttgccgaa gttgacgtgg atgatgtaga tatcggcgac
      241 gtacttttag tgaaaaccgg agccaaagtt cctgtagacg gcaccgtcgt agcgggagaa
      301 ggaagcgtca atcaggccag catcacggga gagtccatac cggtcctcaa agagcagggc
      361 tccaaagtct ttgccgggac tatcctggac aacggcaccc tgcagatcgt agctgagaga
      421 gtaggcgaag acactacctt tggcaggatc attgaactgg tagaagaagc tcaggattcc
      481 aagtcagagg ccgagcgctt cattgaccgc tttgccaagt actacactcc agcagtgctg
      541 gttctggcat ttatcgtttg gctcttttcc aaagatgtag aacttgcaat taccatactc
      601 gtgctcggct gcccgggtgc attggtcatc ggcgttccgg tttcacacgt agcaggcatt
      661 ggcaacggtg cgcgccacgg tattctgctc aagggcagcg aagttatcag cgacttcagt
      721 agagtagata ctttcgtctt tgacaagaca ggcaccttaa cagttggcga acctgaagtt
      781 gcgtacaaag agtactacgg agaagaagtt gaccgagccc taagctacct tgccagcatc
      841 gaaagagagt cggaccatcc gctggctaaa gcagtgctcg aggaaatcgg cgagactcag
      901 ctgtatcctg tggaaaacac ccaagttgta aaaggcggcg ggatcattgc cgatgtggat
      961 ggccattcgg taattgtggg gaacatgtcc cttatggaaa gagaacaagt ggaactggat
     1021 tctaagatgc gcttgagagt cgctgaactg gagcagagtg gccactcgct ggtgcttaca
     1081 gcagtggacc ggcagctcaa agtggttatg ggcgtccgcg atcaggttcg cgagggagtt
     1141 aaagaagatc tgcgaaagct aaaaaaacta ggcgtgaagc atttggtcat gctctcagga
     1201 gacaaccagg gtactgtcga tgcagtgagt agggaactag gactaactga agcccacggc
     1261 aatttgcttc cggaagacaa gtcggcgtat gttaaaaaac taatggagtc cggccgtatc
     1321 gttgctttcg ttggcgatgg ggtaaacgat agcccgtctc tggcttatgc taacattggc
     1381 atcgccatgg gcagcggtac ggatgttgct atcgaaacgt ctgatgtggt tctgatgaac
     1441 tcggatttta gtctgcttcc ggtggctcta ggccttgcga aagccatatc ccgaaacatg
     1501 aagcagaata ttgttatcgc tgtaggggtc gttttggttc tcttgttcag cttgattttt
     1561 ggaagctgga tgaacatgtc catcggcatg ctcgttcatg aaggcagcat tctgcttgtg
     1621 atcctcaacg ggatgaggct cttgcgctac ggtttgaaaa ggtagaagcg attcttgtag
     1681 aagttttcca ggtggaaaac tggatataat gaaagtggaa ataactgagg agaggggact
     1741 gcacaatgaa aacagcaacc attcaattgg agactctgac atgcccttcg tgcatgcaga
     1801 aaattgaagg agcgataaag aaccttgatg gggttgccaa agacactgtc aatgtaatgt
     1861 ttaactccag taaggtgaag ttagagtttg atgaagataa gattgcactc gaagacatta
     1921 caaacgcgat tatcaaggtt ggttacgaag taaaaagcgc aaaatccaag tagacagaga
     1981 aggtgcgtcc ttagtgggcg cacctttcac gttttaagat ctgcaaaaaa gagactgggt
     2041 agtttgtatt atccttgaga attcacgttc cggtgttttt cttgtgcgcc attctcatgt
     2101 tataataatt gcatgtaaaa gcggtataaa ggcagttgta gagtgacgtt acttgaggcc
     2161 tggtggtgca cctctcacga aatacatagc tgttactgga gaaaggggac tggctcgtga
     2221 aaaagatctt tgagggtaaa actaaggacg tctatctctt agacgatggc aattaccggt
     2281 tgaaattcaa agacgatgtt acaggtgaag gcggggtctt tgatccaggc gcaaatactg
     2341 taggacttac catcgaaggt atcggcaaga gaaatctcga ggtttcagtg aggttctttg
     2401 aaatactgca gaaggcaggt atcaaaaccc attacattgg agccgatatt gaaaatggaa
     2461 cgatggacgt gaagccggca aaatcgccgc ggtcaaccat ggcgacccgg gcgccgcttt
     2521 aaggcagtag ggagtttcct gcgccgttac ggcgggtatg tgaacgaagg cgacgatctc
     2581 gacggatacg tagaagctac tctgaaggac gacagaagag gagacccctt ggttacctca
     2641 gaaggtctag aagtccttgg cattatgtcg caggagcagt ttgcccagat caaggcggaa
     2701 accagaaaaa tcgcagacct gatccgcagt gaaatcggga aaaagggact ggagctctac
     2761 gacattaagt tcgagtttgg cctagatgca gacggcagtg ttatgcttat tgacgagatc
     2821 tcatcgggca atatgcgcgt ctataaaggc ggcacgatca tggaccctct ggatcttacg
     2881 gcaacgcttt tgtccgaacg gtagaagtgt tctagcaatg cctttgtgac gatggagaac
     2941 gctacgatcg acacttaacg acgcactctg caggatggtc ctttcagagt gctttttttc
     3001 tttcgctggg agcaggtgga aaggcgtgaa gtgtttaccg cgttatttgt tttgatttga
     3061 gaagggtttt gccccgcata atcgtgaatt tattcatgta caaagttgta catcgaatgc
     3121 aataacaacc cctgctcgca tgccgagctt taagcaaaaa ggagtagaag tgatggataa
     3181 gctaagaaag ccgagaaaat cgctgctttt ctactacggc atcttcatac tgattctggc
     3241 gattttcaac ttcatggtcc tgccctggat tttggaaagg cagatcatcg aggttgacta
     3301 tcgcactttc atcaagatga ccgaagaaaa tcaaatcggt caggttcaga ttcagcagaa
     3361 tcggatactg ttcactgaca aagaaaacac tatggtgttc aagacaggtt tgatggatga
     3421 tcccaaccta gtggagcggc tttataaatc tgatgctgtg ttttccggtc aaatagtgga
     3481 acagatgtcc ccaataatga gcattttgct ttcgtgggtt ttgcccatgg tgatcatttt
     3541 cgtggtaggt cagtattttg ccaagaagat catggacaaa accggagctg gcaacgcact
     3601 gacctttggc aaagctaatg ccaaagtgta cgttcagtct gaagagggta tcaagttcag
     3661 cgatgttgca ggtgcagacg aagcaaaaga gaatcttgct gaaatcgtgg actacctaca
     3721 caatccccag aaatacaggg agatcggtgc ttccatgccg aagggagtgc ttctcgttgg
     3781 ccctcccgga accggtaaga ccatgctggc aaaagcagta gcaggcgagt ccaacgtgcc
     3841 gttcttctct attgccgggt cggagtttgt agagatgttt gttggccttg gcgcttcgaa
     3901 agtgagagat ctcttcaagc aagctaaaga aaaagctccc tgcatagtat ttatcgatga
     3961 gatcgatgcc attgggaaaa agcgcgacgg ctctgttggc ggcaacgatg agagggagca
     4021 gaccctcaat cagcttctca ccgagatgga cggttttgaa gagaacacag gtgtgatcat
     4081 tctcgcagcc acaaaccgtc cagaggctct cgatcctgct ctaacccgcc cgggaagatt
     4141 tgacaggcgt attcctgtag agcttcccga tctgaaaggc cgcgaagcca tcttgaaggt
     4201 ccatgcaaga aagattaagc tggccgaggg agtagacttt gaaaaagtag cccggatggc
     4261 atctggtgca tctggtgcag aacttgccaa cattatcaac gaggcagccc tgcgagcggt
     4321 ccgggaaaac cgcatgctgg ttacccagga tgaccttgaa gcgagcatcg aagtggtcat
     4381 cgcggggtac gaaaagaaaa acgctatctt gtccgataag gagaagtggg cagtggctta
     4441 ccacgaaata ggtcacgctc tggtggcagc aaagcagacg aactcgcctc cagtgcaaaa
     4501 gatcacgatt ataccccgca cttccggagc ccttggctat accatgcagg ttgacgaagg
     4561 cgataagtac cttatgacca aggaagatat cgaaagcagg atagctacct ttacaggggg
     4621 acgggcagca gaagaagttg cactgggcac catgactaca ggtgcgtcca acgatatcga
     4681 acaggcaaca aggcttgcca gaagcatgat cacccgcttt ggcatgagcg aagagtttgg
     4741 catggtagcc atggagactg taaccagtca gtatttaggc ggtgacacgt ctctagtatg
     4801 ctcagcagaa acacaggcaa agatcgacag agctaccgcc gatatcatcc gcagacagta
     4861 cgaaaaggcc aaaagaattc tcgaagaaaa caaagagaaa cttgatgagt tagcgaaata
     4921 tctatatgaa aaagagacga taaccggcgc ggagtttatg gagatactgg ggtcgtagcc
     4981 tcgagaaagt ggtatggcag atcggcagta ggtaaaagac aagaccgccc ggtctttgag
     5041 gaggttgaag tgcgtgttga ctctatggct cttcctgccc ttaggtgcta tctttgtgtt
     5101 catcggtttg gcggttcacg tctttaagtg gtattctatg atctctggat acaacaccat
     5161 gtccaaagag cagaaggcaa atgtcgatgt agagggtttg tctaaagcag tgggcatctt
     5221 ctgctatgtt aacggcggac tgttcctgct tacaggcata ctttcagcgc tcggcgtaaa
     5281 agtgtcggtg ttcattcctt ttggcgttct gctcgtttca acaccgcact tcgtcagttc
     5341 tgtccgcaag tacgacaaaa acaccgaaac gcaaaaagct gccaaaggcc tcaactggac
     5401 tatgatgatc actttagtcc tggtagcagt tctgttcttt ttcgctactc gtcccacaaa
     5461 catcaatgtc ggcgatgaag gtatccagat tacgggcctt tacggaggaa cctacacctg
     5521 ggaatccatc gagtcagtaa gtctagaaga cactctcccc aagattgaga tgcgcacgaa
     5581 cggttccaaa gtgggctcgc atctcaaggg ttacttcaga acgaccgagt tcggccaggt
     5641 caagatggca ttagacgctg cgaaaccgcc tttcatctac atgaagacag acggcaaaat
     5701 gtttatcttc aacgccggcg actccgagaa aacccgtcag ctttacgagg aaatcgcaga
     5761 tagactccaa tagtctccta accaatatca tcggtgtcat gaaagttgtt atagcaaaga
     5821 ctgtagagat gcttgacgac tcggtgcagg tacagctcaa gaaggaaaag agctgagtta
     5881 ggagaaactt ccttaacgaa aagaaacacc atgaaaaaca ggcagtactc gttacccata
     5941 ggagacgaag tactgccttt tatattagaa cttgctttct ttttgttcgc cggctctaat
     6001 tccgggatgc aggcggtcaa gaccttcttc cctgaagggg ttgataacgc caattttctt
     6061 accttcacgt tctactttga tttcccaggg cggcttgcgg tagaacgggt tgatgaatgt
     6121 attttggatt tttcctgctt tttccagtga atccatacaa gctctgat
//
LOCUS       DAOSDA010000040         6129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_8169_length_6129_cov_3.437932, whole genome shotgun sequence.
ACCESSION   DAOSDA010000040 DAOSDA010000000
VERSION     DAOSDA010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8169_length_6129_cov_3.437932"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..121)
                     /locus_tag="PLW82_01665"
     tRNA            complement(35..121)
                     /locus_tag="PLW82_01665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(86..88),aa:Leu,seq:taa)
     gene            complement(183..1538)
                     /locus_tag="PLW82_01670"
     CDS             complement(183..1538)
                     /locus_tag="PLW82_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HOJ46013.1"
                     /translation="MKQRVADQLGRKPQGKYWIHKTCPWGYPIVLKNHPYEGAISPTL
                     YWLSCPYLVKFVSGLEAEGMVGEYNQRIIEDPEFKRKMENAHKSYSKERSELIEPDIV
                     LDDKVLSKLRTSGVGGSESWDGAKCLHMHLGHFLANGENPIGEEVWEMVTPWKAEDCP
                     ENCPPVPTAGCKPLAVIDAGSNTCRLLIAGFYPQPLKLPIISEGEDGYLQAMLTEVIT
                     TEAGRDIETGREKTRWAVERFLSLIEEFGAELVGGVATGIWRHVGTPHDKLKVISGRD
                     EANFSYLGAIGSLDLKGEVAVCDLGGGSLEIVRGTGTDVRELDSFDVGFWSVAKALNL
                     QKSSLSEVDAQRIRDYVKEKLGSIEPVDQMVFIGGTATTLAGLKQELDEYDAFRIHGF
                     EIDPDFPFDVDNLPSWAAERKASIAIGAEIIKAVLELAGKSSAVVSDLGLMAGILCSS
                     Y"
     gene            complement(1589..2017)
                     /locus_tag="PLW82_01675"
     CDS             complement(1589..2017)
                     /locus_tag="PLW82_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003217901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOJ46014.1"
                     /translation="MSIEVGSVVEGKVTGITSFGAFIDLGNGQTGLVHISEVADDYVQ
                     DINNYLKINDVVKVRVLSMKDGKIGLSIRKVDSVNKERSRRSSNGNTKNQGQPDKNFE
                     SKLLKFLKDSEARQKDLKKNQDSKRGGRGSARISANNNLY"
     gene            complement(2100..2459)
                     /locus_tag="PLW82_01680"
     CDS             complement(2100..2459)
                     /locus_tag="PLW82_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOJ46015.1"
                     /translation="MARKRFKPNLLQLCLLIFLLVSFAWNMYKIVTAQKMINQLKNEI
                     EKVQKTVSDIRYKQQLVEEEIARWNDPKVIEEFARKELGFVRPGEVTYMFSEGVQPAT
                     DSDVAKRSSSSREGIQN"
     gene            complement(2483..2860)
                     /locus_tag="PLW82_01685"
     CDS             complement(2483..2860)
                     /locus_tag="PLW82_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46016.1"
                     /translation="MLGAIWGFVWEFLEALALGGRKIAVLLVVFMLPISAVLWYISNN
                     GALRYYVPISVAVGILVFSLISRQTIGATIALAVKRIIGTIWLILNIVWIIFKFSLES
                     VGLLTYAIVRKISISKSKAAVIN"
     gene            complement(2967..3254)
                     /gene="yabP"
                     /locus_tag="PLW82_01690"
     CDS             complement(2967..3254)
                     /gene="yabP"
                     /locus_tag="PLW82_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HOJ46017.1"
                     /translation="MAIEERKVGQLKNHQVVLKNRQELNIEGVVSVESFDDQEVVLET
                     EEGLLVIRGENLNVSQLNIDAGKLNVTGSVRILEYLGVSGQKSGFFSKFFR"
     gene            complement(3303..4223)
                     /locus_tag="PLW82_01695"
     CDS             complement(3303..4223)
                     /locus_tag="PLW82_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOJ46018.1"
                     /translation="MKRVLIAVCLAVCFLAAGCGKKDTLSKEPTITLYITKTGEKKTM
                     PLEEYLYGVVAGEMLPNWPKEAYAAQAIIARTFTMEFLERGGVKDKYGTDLSDDITET
                     QAYNPDAISDVIKAAVDETKGLVAKHQGNYIKGWFHAFSGGQTTTAKVGLGYKNPEPP
                     YIKVVDDPGTEYAPPDNRNWTASYTYAELTGLLKEKGYVNFDVSGVKIGGTNDQGRVT
                     EFVVTGDGTSKTVPGAEFRIALDSTKLKSIWVTGIEETNSGVVFSGKGYGHGVGLSQW
                     GAYALAQKGSSAEDIVKYFFNDVDIVQAYK"
     gene            complement(4262..5569)
                     /gene="mazG"
                     /locus_tag="PLW82_01700"
     CDS             complement(4262..5569)
                     /gene="mazG"
                     /locus_tag="PLW82_01700"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOJ46019.1"
                     /translation="MIKVVGLGIGNLDDLSRKAASLLQSTSRLITTASWHPVFSSLDK
                     AHIEVWDGFSPEEITSRLNQVGEGEDVVLAVSGDPALDHSYLRGIKNKLELLGAPSLL
                     ALLELPEAFLQLDAESLKSAEDFGAKVVRVHPGSWEQIKKALPQDADVVCVDKLQVGK
                     GKVEQIYIEDGYVFIPGYVAVKKTSAIDALLTTMRKLRAPDGCPWDRIQTFESLKPYL
                     VEEAYELWDAIDSGDMDLVTEELGDVLLQVVFYAAIAEEEGLFDFEDVARVVNEKLIH
                     RHPHVFAEVVADTPEKVMANWERLKKEEKGKEDRVSILDGVPKHLPGLQKAAKVQKKA
                     AKVGFDWDGIEPVWQKVSEELQELSEAKTPEEREEELGDVLFAIVNLSRFLKIDAEVA
                     LSKTIAKFQKRFRYIEKHADRPLDEMTLEEMDVYWEKAKEEDK"
     gene            complement(5569..>6129)
                     /locus_tag="PLW82_01705"
     CDS             complement(5569..>6129)
                     /locus_tag="PLW82_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46020.1"
                     /translation="MLEEALQQRKEGQVEAEEAFEPQINITLDGYLPSSYIQNEGQKI
                     EMYKKINAVSSRRELMDIEDELLDRYGTLPEEAENLLWFCHLKLIAHELLIEEIVEQR
                     DVARGRKSLVVRFRPDAPLDAGRIQKVWEKYKTSIGFIHGKAPQIKVHRWDAKDWNAR
                     RDLLDILEILMGAKEVTKKQESGVLH"
BASE COUNT         1440 a         1683 c         1267 g         1739 t
ORIGIN      
        1 aaaagggggt tgaatccttg ttgccccaaa aaaatggtgc gaggagaggg agtcgaaccc
       61 tcacggccta ttaggccagc ggattttaag tccgctgcgt ctgccattcc gccaccctcg
      121 cacattacac aaaacattct actacattcg ccatggatgg tcaattggca tttcaacctt
      181 tgtcagtagc tagaacataa aattcctgcc atgagcccca gatcactaac aaccgctgaa
      241 gattttcctg caagctccag cacggccttg ataatctcag caccaatggc aatacttgcc
      301 ttcctctccg ctgcccaaga gggcaaatta tctacatcga atgggaaatc cggatcaatc
      361 tcaaatccgt gtatccggaa ggcatcgtat tcatcgagtt cttgcttcaa acccgcaagc
      421 gtagttgctg ttccaccaat aaacaccatc tggtccacag gctcgatgct tcccaacttc
      481 tccttgacgt agtccctgat tctctgtgca tcgacctcag aaagactgga cttttggagg
      541 ttcagagctt tcgccactga ccaaaaaccc acgtcaaagc tgtcgagttc acggacatca
      601 gttccagttc cacggacaat ctcaaggctt cctcctccga gatcacacac tgcaacctca
      661 cccttcaagt ctaaagagcc tatggctccc aaataggaga agtttgcttc atcacgacct
      721 gagatgacct tcagcttatc gtgaggagtg ccaacgtgtc gccaaatgcc ggtcgcaact
      781 ccccccacaa gctcggctcc gaactcctct attaaactca gaaagcgctc cactgcccac
      841 cgcgtctttt ctcttccggt ttcaatgtct cgccctgcct ctgttgtgat aacttcagtc
      901 aacatggcct ggaggtagcc gtcttcgcct tcgctgataa tcggaagctt tagcggttgg
      961 ggataaaaac cagcaatgag caatctacac gtattacttc cagcatcgat caccgccaaa
     1021 ggcttgcaac ctgctgtagg aactggcggg cagttctcag ggcagtcttc tgctttccag
     1081 ggagtaacca tctcccacac ttcctccccg atggggtttt ctccgttagc taggaagtgt
     1141 ccgagatgca tgtgcagaca ttttgccccg tcccagcttt ccgatcctcc aactcccgac
     1201 gtcctcagct tactaaggac cttatcatca agaacgatgt ccggttcaat gagctctgag
     1261 cgttctttgc tgtaagactt gtgagcattt tccatcttcc tcttgaactc aggatcttct
     1321 attattcttt gattgtactc gccaaccata ccttctgctt ccagtccgga gacgaatttc
     1381 actaagtacg gacacgacag ccaatacaaa gttggcgaga tcgccccttc atagggatgg
     1441 tttttcagga ctatgggata gccccatggg caggttttat gaatccagta cttgccctgt
     1501 ggttttcgac ctaactgatc tgctacgcgc tgtttcattt tctcctccca caaaaaaagg
     1561 cacccaatcg cggatgccct ttagttcttt agtaaagatt gttatttgcg ctgatgcgag
     1621 ccgaccctcg tccaccacgc ttagaatctt ggtttttctt cagatctttt tgtctagctt
     1681 cactgtcttt caaaaacttg agaagcttag attcaaagtt tttgtcaggt tgaccttgat
     1741 tcttggtgtt gccattgcta ctacggcgag agcgttcctt gtttacgcta tcaaccttgc
     1801 gaatagaaag accgattttc ccatctttca tggacaaaac tcgtactttg acaacatcgt
     1861 ttatctttaa gtagttgtta atgtcttgga cgtaatcgtc cgcgacctct gagatatgaa
     1921 ctagccctgt ttgaccgttt cctagatcaa taaatgctcc aaaacttgtg attccggtaa
     1981 ctttaccttc tacaacgctc ccgacctcaa tggacatact agaagggact cctcctcaaa
     2041 aatattaagc gatatgcttt ttcatagtat attataccag cagcaatgtc aaagtcaact
     2101 cagttttgaa tgccttcacg gcttgagctg ctgcgtttag cgacatcgct gtcggttgca
     2161 ggttgtacac cctcgctaaa catgtaggtt acttcgcccg gtctgacgaa ccccaactct
     2221 ttcctggcaa actcctcaat cacctttgga tcgttccacc tggctatttc ttcttcaacc
     2281 aactgctgtt tatacctgat atcactgacg gtcttttgaa ccttctcgat ctcgttctta
     2341 agctggttaa tcatcttctg ggcggtaacg atcttgtaca tattccaagc gaagctaaca
     2401 aggaggaaga ttaacagaca aagctgcagt agatttggtt taaatctctt tctggccatc
     2461 tgtaaccccc tcccctctgt ttttaattaa tcacggctgc tttcgacttg gaaatgctga
     2521 ttttcctgac tatagcataa gttaataaac cgacactttc aagagaaaac ttgaaaataa
     2581 tccaaacaat atttaatatt agccaaatag tgcctattat cctttttact gccagagcaa
     2641 ttgtggcgcc gatagtctgc cttgagatca acgagaaaac caaaataccg actgcaacgc
     2701 taatgggcac atagtacctc agggcaccat tgttgctgat gtaccagaga acagcagaga
     2761 tcgggagcat gaacacaacc aggaggactg cgattttcct ccctccaagg gcaagagctt
     2821 ccagaaactc ccagacaaat ccccatattg caccgagcaa caaaagatac ccgatagaaa
     2881 tcagctgcga ggccaaaggc atcattatgg aaaactccct cctaactagc tctccatact
     2941 atgcctttgg ccctattagt gctactctat ctgaaaaact tgctgaaaaa ccccgatttc
     3001 tgtccgctga ctccgaggta ctccagaatc cttaccgagc cggtaacgtt cagctttccg
     3061 gcatcgatgt tcagctgact aacgttgagg ttttctcctc tgatcacaag gaggccctct
     3121 tcagtttcca ggaccacctc ttgatcgtca aaactctcta cactgacaac accttcaatg
     3181 ttcagttcct gacgattctt tagaacgacc tggtggtttt ttaactggcc gacctttctt
     3241 tcttcaatag ccataatctc tccccctcac tcaagcttat tacagaggga ggacaggtat
     3301 gactatttat atgcttgcac tatgtcaacg tcattgaaaa aatacttaac aatatcttcg
     3361 gccgagctgc ccttctgtgc aagtgcatag gctccccatt ggctgagccc cacgccatgg
     3421 ccgtagcctt tccctgagaa caccacacct gagttggtct cttcaatgcc cgtgacccag
     3481 atagatttca gcttggtaga gtcgagagca atgcgaaact cagctccagg aacggttttg
     3541 gatgtgccgt ctccagtcac cacaaactcc gtgacccttc cctggtcatt ggttccgccg
     3601 atctttactc cagacacatc gaagttaacg tatcccttct ctttcaaaag tccagtgagc
     3661 tctgcgtaag tatagcttgc ggtccagttg cggttgtccg gtggtgcgta ttcagttccg
     3721 ggatcatcca ccaccttgat ataaggaggc tctgggttct tatatcccaa acccacttta
     3781 gcagtggtag tctgtccgcc ggaaaacgcg tgaaaccatc ccttgatgta gtttccctgg
     3841 tgcttcgcca caaggccttt ggtttcatca acagcagctt taattacatc ggatatggca
     3901 tctggattgt aggcttgagt ttcagtgatg tcgtctgaga gatcagtgcc gtacttgtcc
     3961 tttactccac cacgctcaag aaactccatg gtgaaggttc ttgcgattat ggcctgtgca
     4021 gcataggcct ctttaggcca gtttggcagc atctcgccgg caacgacgcc atagagatac
     4081 tcctccagag gcatcgtctt cttttctcct gtcttggtga tatacagtgt aatagtcggc
     4141 tcctttgaaa gcgtgtcctt tttgccgcat cctgcagcaa gaaaacagac cgcaaggcag
     4201 actgctatca atactctttt catgaatacc cctccaagag gtattctgca taatcttcgc
     4261 cctacttatc ctcttctttg gccttttccc agtacacgtc catctcttct aaggtcattt
     4321 catctaacgg cctatctgca tgtttttcga tatatctgaa cctcttctgg aatttggcaa
     4381 tggtcttact tagtgccact tcggcatcga ttttcaaaaa ccgcgaaagg ttgacgatgg
     4441 caaaaaggac atcgcctaac tcttcttctc tttcctccgg cgttttggcc tcggacagtt
     4501 cttgcagttc ttccgagacc ttctgccaca ccggttcgat accgtcccag tcgaagccca
     4561 ctttggctgc cttcttctgt acttttgcgg ccttttgtaa ccctggcagg tgtttgggta
     4621 cgccgtccag gatggaaact cggtcttcct tgcccttttc ttcctttttt aagcgctccc
     4681 agtttgccat gactttttcc ggggtatctg cgacgacctc ggcaaaaacg tgggggtgcc
     4741 tgtggatcag cttttcattg accaccctag ctacgtcctc aaagtcgaac aagccttctt
     4801 cttcggctat ggccgcgtag aacacaacct gcagtaaaac atcaccaagc tcctcggtga
     4861 caagatccat atctcctgaa tcaatggcat cccatagttc gtaggcttct tcaacaagat
     4921 acggtttgag cgattcgaag gtctgaatcc tgtcccacgg acagccgtcg ggagctctca
     4981 gctttctcat ggtagttaga agagcatcta tggcagaagt cttcttgact gcaacatatc
     5041 ctgggatgaa aacataaccg tcttcgatgt agatctgttc tacctttcct tttcccacct
     5101 gaagcttgtc cacacagacc acgtctgcat cttgcggcag ggcttttttt atctgctccc
     5161 aactccctgg atggaccctg acaactttgg ccccaaagtc ttctgcgctc ttcaagcttt
     5221 ctgcatccaa ttgaagaaat gcttcaggga gctccaatag cgcaagaagc gacggagctc
     5281 ctagaagttc tagcttgttt ttaatccctc gcagatagga atggtcaagg gctgggtcac
     5341 cggacactgc gagaaccaca tcttctccct ctccaacttg gttcaatctg ctagtgattt
     5401 cttctggcga aaagccgtcc caaacttcaa tatgggcttt atccaaactt gaaaaaacag
     5461 gatgccagct tgctgttgtg atgagacgag atgtactctg cagcagggaa gctgctttcc
     5521 tgctgaggtc gtccagattg cctattccaa gtccaactac ttttatcatt aatgcagaac
     5581 cccgctttcc tgcttttttg tcacttcttt cgcacccatc aagatctcca aaatgtcgag
     5641 gagatcgcgt cttgcgttcc agtctttggc atcccaccgg tggaccttga tctgaggcgc
     5701 tttcccgtgg ataaagccga ttgaggtctt gtatttctcc cagacctttt ggattctgcc
     5761 cgcatccagc ggagcatcag gtctgaagcg aactactaaa ctcttcctgc cccgggcgac
     5821 atccctctgc tcaacgatct cttcaataag gagttcatgg gcgatgagct tcaggtggca
     5881 gaaccagagc aagttctccg cttcttcggg gagagtgcca tagcggtcga ggagctcatc
     5941 ttcgatgtcc ataagttctc tgcgggaaga aaccgcattg atctttttgt acatctcgat
     6001 cttctgacct tcgttttgga tatagctgct ggggagatac ccgtcgaggg tgatgttgat
     6061 ttgcggctcg aaagcttctt ctgcttcgac ctgtccctct tttctctgct gcagcgcttc
     6121 ttcaagcat
//
LOCUS       DAOSDA010000041         5988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_8407_length_5988_cov_3.167706, whole genome shotgun sequence.
ACCESSION   DAOSDA010000041 DAOSDA010000000
VERSION     DAOSDA010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8407_length_5988_cov_3.167706"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..94
                     /gene="rrf"
                     /locus_tag="PLW82_01710"
     rRNA            <1..94
                     /gene="rrf"
                     /locus_tag="PLW82_01710"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            220..837
                     /locus_tag="PLW82_01715"
     CDS             220..837
                     /locus_tag="PLW82_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46021.1"
                     /translation="MKKFLILLTILVLIAQPLMAASFNATHKSILEAKLEKLLEIDGN
                     DVRSMTLAAIALKEYLNLVAENLILLSEDEANNAVVEDLYVDFLFGRELYNTLVARVT
                     EMVQEDVWHLELDPPLHVSPDLQQGAAAYSVYLVEIARGLYPESYEKVHEAILNYLPI
                     AYQNRLFLLSVDAPTQDEIARILLSEETAQLSLDLQKTISALSGR"
     gene            1038..1274
                     /locus_tag="PLW82_01720"
     CDS             1038..1274
                     /locus_tag="PLW82_01720"
                     /inference="COORDINATES: protein motif:HMM:NF022223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor G inhibitor Gin"
                     /protein_id="HOJ46022.1"
                     /translation="MARCLICGSEVDSILVIGKHAICGNCEDAILNRNPNDKEYEELL
                     AVFKPLSQEILASAEKTAETTEQGTIEREIKDEE"
     gene            1264..2703
                     /locus_tag="PLW82_01725"
     CDS             1264..2703
                     /locus_tag="PLW82_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46023.1"
                     /translation="MKSKKVLDQTKTPFLDAVERYVEKGLVPFHTPGHIQGRDILERA
                     SNLYSQPLLYDLAEVDYPEGAHDYEIARKEAEILLADLYGMERSFFLINGTTEGIHAL
                     FMAFFSQDGTQKKILLPRNSHRAAVGGLILTGAKPVWVYPEIDSELGLATVCRTEDWL
                     EHTDVDGAFHLYPNYYGLCQNLDEQIISGVYNLVDEAHGPHLRFSERLPKSAIDSEIT
                     ASVQSAHKILTALTQSSWLHVKSKDAILAIERALWLIESTSPNFILWSSLDAARALEA
                     KNPDRWDQALDRVLTFREKLKNIPGLTLFEPGVNHPEVHSFDPFKLVVSGRKAGLNGF
                     ALRDELRLRGIVPELAEPGFVLFLLTPAHTQKDLGFLFDALQHIFKGRKPVEGNTEQV
                     FFYPRLNAHLTPKEAYFAESRKVSLRDSAGLVSVEMITPYPPGIPLLMPGEEITKEVI
                     ELIQYYKELGMQLRGFEDEKAEFIRVVAK"
     gene            2705..4045
                     /locus_tag="PLW82_01730"
     CDS             2705..4045
                     /locus_tag="PLW82_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ46024.1"
                     /translation="MTQKVTNDLDVFLGVLPKRISEVLEKDPELDELLEVVLDLGRLP
                     EARFPDRFVYLGNAVVTRQELSEVISKVGEFGSDNRAGIERTLHRISSLRNRRGEIIG
                     LTCRVGRAVMGTIDIISDIIKKGQSILLLGKPGVGKTTMLREIARELAERKRVVIVDT
                     SNEIGGDGDIPHPGIGRARRLQVPRHDLQYQVMIEAVENHMPEVVVIDEIGNAYEAEA
                     ARTIAERGVMLIGTAHGLSLKNLVSNPALVDLVGGVQTVTLSDEEARRRGTQKSIQER
                     KQEPTFEIVIEIVSREELIVIEDVGQAIDKLLMGSTYPQEIRRRTPDGIVKEQEFEKT
                     KKRHREFRNIYPYGISRNKLQVAISELKAPMNVVRSIKDADIVLTLKGQYTRLPADIK
                     EAEFKGLPVKVIRSNTAIQIMNALAEIAPPNGDRQIIDKMVESGRKALGRNTDA"
     gene            4075..4737
                     /gene="tmk"
                     /locus_tag="PLW82_01735"
     CDS             4075..4737
                     /gene="tmk"
                     /locus_tag="PLW82_01735"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOJ46025.1"
                     /translation="MAGLFLSFEGIDGCGKSTQADLCFRALQEMNIPAVKTREPGGEP
                     VAEKIREILLYSKDEIAHSTEVLLYAASRAQHVARVIRPSLEAGKVVVCDRFVHSSLA
                     YQGYGLGIDIDAIWNINYFAMGGTFPDLVFLFDIDLEEARSRSKARENSSGREISSNN
                     EINNREDRIEKRDLVFYCKVRLGFLELAKNDSRIIVLDGREPIDILHGKVMKAVTRAL
                     NV"
     gene            4784..5116
                     /locus_tag="PLW82_01740"
     CDS             4784..5116
                     /locus_tag="PLW82_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOJ46026.1"
                     /translation="MKLVVAVVQDQDAPLLLDKLSEEKIRATKLASTGGFLKQGNTTL
                     LIGVNENLVDTVLDIIRRTCSSKEQLITPVVSDFSRSVPDFIPQPVSVKVGGATVFVL
                     DVHHFEKV"
     gene            5124..5573
                     /locus_tag="PLW82_01745"
     CDS             5124..5573
                     /locus_tag="PLW82_01745"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HOJ46027.1"
                     /translation="MGVKIGPKSGMIRTDVELKESTKLKVSNTDFLDLLKRDSGKNTQ
                     QELEKLLQEIDSLGAALAEDLSLKTLKRYREAIKQFISYAIKGSIEVLDQSGIDIYGS
                     PRSYKIIRKVDDSLEQLAQAILKRHEAQIEIVARVSEIRGLLVDLMG"
     gene            5641..>5988
                     /locus_tag="PLW82_01750"
     CDS             5641..>5988
                     /locus_tag="PLW82_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ46028.1"
                     /translation="MFERVVGQESSKRILTAAVENDGISHAYLFHGPKGVGKTTMAVE
                     LAMVLNCEERNGCGVCSSCRRIKEGIFPDVKMVAPDGPSIKIGEIRELQNFIAMSSFE
                     GRFKVGIIQDAEKL"
BASE COUNT         1732 a         1316 c         1534 g         1406 t
ORIGIN      
        1 cccacctgtt cccattccga acacagaagt gaaacgcttc agagccgatg gtactacagt
       61 ggagacgctg tgggagagta ggtcgttgcc ggatcttttt ttacccctca ggtttactcc
      121 tgaggggctt tttccagcga ccttgcgtcc aatttctcca catttttgca gggtttaggc
      181 gagttttatt gaaattgcca agagcggagg tgtacaccga tgaaaaagtt tttgatccta
      241 ctgaccatac ttgtcctaat agcccaaccg ctcatggcgg cttcatttaa cgctacacac
      301 aagtccatcc tcgaagccaa gctggaaaaa ctccttgaga tcgatggaaa cgatgtaagg
      361 agcatgacgc tagctgctat agcactgaag gagtacttga acctagtagc agaaaaccta
      421 atccttttgt ctgaagacga agcaaataac gccgtcgtgg aagatcttta tgtggatttt
      481 ctgttcggca gggagctata caacacatta gttgcgagag taacggaaat ggtgcaagaa
      541 gatgtttggc acctagaact ggatcctccg cttcacgtaa gccccgatct ccagcaaggt
      601 gctgcagcct atagcgttta tcttgtggag attgctagag gcctttaccc tgaaagctac
      661 gagaaggtac acgaagcgat tttgaattac cttcctattg cttaccagaa tcgcttgttt
      721 ctcttgtctg ttgacgctcc tactcaggac gaaatagccc gaattctgct ttctgaggaa
      781 actgcacagc tgtctttaga tctgcagaag acgatcagcg cactatctgg taggtagcgt
      841 tttctctaca ccttatgcgg tagctgctgt actttgccat ctcagatcct cggtgaagcc
      901 gacgcttgcc tatccactct ggttgatggg gaccgaaaac cggtcccttt cttgtttata
      961 gtgtagaggg gaaaagaata agcgaagtcg taatatttaa gtgaatacta agtacgaagg
     1021 aaggtaggtg ttttctaatg gcaagatgtc tgatatgcgg cagcgaagtc gatagcattc
     1081 ttgtcattgg aaagcacgct atctgcggga actgcgaaga cgctatctta aaccgaaacc
     1141 ctaacgacaa ggagtacgag gagctccttg ctgttttcaa gcctctgtct caagaaattt
     1201 tggcttcggc ggagaaaacc gctgaaacca cagaacaagg taccatagaa agagagatta
     1261 aagatgaaga gtaagaaggt tttagaccaa accaagactc ctttcctgga tgcagtggaa
     1321 aggtatgtag aaaagggttt agtgcccttt catacccctg ggcacataca gggaagagac
     1381 atcctagagc gtgcaagcaa cttgtacagc caaccgctcc tatacgatct tgcagaagtg
     1441 gactacccag agggagccca cgactacgaa atagcaagaa aagaagctga gatactcctt
     1501 gcagatctct acggcatgga acgttctttc tttcttataa acggcaccac agagggcatt
     1561 cacgctttgt ttatggcctt cttttcccaa gacggaactc agaagaagat tctccttcct
     1621 agaaacagcc acagagcagc tgtagggggg ctaattctca ctggagcgaa gcccgtttgg
     1681 gtgtaccctg aaatcgactc cgaactgggc cttgccacag tgtgcagaac cgaagattgg
     1741 ctggagcaca cagacgttga cggagcattc cacctttatc ccaactacta cggattgtgt
     1801 cagaacctgg atgagcagat cataagcggg gtatataacc tagtagacga agctcacggt
     1861 ccccacctta gattttccga gaggctgcca aagtctgcta tagacagtga gataaccgct
     1921 tcggtccaga gcgcacacaa gattctgact gcacttaccc agagttcatg gctgcacgta
     1981 aaaagtaaag atgcaatcct tgccatcgaa cgagccctgt ggctcatcga aagcacaagt
     2041 ccaaacttca tcctctggtc gtcgttagat gcggcaagag ctcttgaagc aaagaatccc
     2101 gaccgttggg atcaggcttt agatcgcgtt ttaaccttta gagaaaaact gaaaaatata
     2161 cccggactta cgctgtttga gcctggagtc aatcaccctg aagttcacag cttcgatcct
     2221 tttaaactag tggtaagcgg cagaaaagct ggcctaaacg gatttgccct tagagatgaa
     2281 ctgagattga ggggaatagt gccggaactt gccgagccgg gttttgtgct ttttcttctc
     2341 actccggctc atacccagaa agatctaggt ttcttgtttg atgctctaca acacattttc
     2401 aaaggcagaa aacctgtaga ggggaacaca gagcaggtgt ttttctatcc gaggttgaac
     2461 gcacacctga ccccaaaaga ggcttatttt gcagaaagta gaaaagtgtc cttacgagat
     2521 tcagcaggtc tggtgtctgt ggagatgatc acgccctatc ctcctggcat accgcttttg
     2581 atgcccgggg aggagattac taaagaagtc atcgaattga tacaatacta caaagagctc
     2641 ggaatgcaac tccgcggttt tgaagacgag aaagcagaat ttataagggt ggtagcgaag
     2701 taaaatgacg caaaaggtca caaatgactt ggatgtgttt ctcggcgtac tgccaaaaag
     2761 aatcagtgaa gttctggaaa aggatcccga actcgacgag ctccttgagg tagtgctgga
     2821 tcttggaagg ctccctgagg cacgctttcc cgaccggttt gtctacctgg ggaatgctgt
     2881 tgtaaccaga caggagcttt ctgaggtcat atctaaagtt ggcgagtttg gttccgacaa
     2941 cagggcaggg atcgaaagga ccctgcatag aatatccagt ctgagaaacc gcagaggaga
     3001 gattatcggt cttacctgcc gagtcggtcg cgcagttatg gggacaatcg atattatttc
     3061 cgatataata aaaaaaggtc aatccatact gcttttggga aaacccggtg tgggtaagac
     3121 gaccatgctc cgagagatag ctagagagct ggcggaaaga aagcgtgtag ttatagtaga
     3181 cacctccaac gaaatcggtg gagacggcga catcccccat ccgggaatcg gcagagctag
     3241 gcgtctgcag gtacctaggc acgatctgca gtaccaggtt atgatcgaag cagtggagaa
     3301 ccacatgccg gaagttgtgg tcatcgatga gatcggaaac gcctacgaag cagaagcagc
     3361 aaggaccatc gcagaaagag gcgtgatgct catcggcact gcccacgggt tgtcgctgaa
     3421 aaacctggtt tccaatccgg cgttggttga tctagtcgga ggagtccaaa cggtcactct
     3481 cagcgacgaa gaagcccgta gaagagggac ccagaagtct attcaagaaa gaaaacaaga
     3541 gccaaccttt gagattgtaa tcgagattgt cagtagagaa gaactgatcg ttatcgaaga
     3601 cgttggccaa gcaatcgata aactactgat ggggagcacg tatccccaag agattcgcag
     3661 aagaacacct gatggtatcg taaaagagca ggagttcgag aaaaccaaga agcgccacag
     3721 ggagtttaga aatatctatc cttacggcat cagccggaat aaactccagg ttgccatctc
     3781 ggagctgaaa gctccgatga atgtggtgcg aagcatcaaa gatgccgata tagtattaac
     3841 ccttaaggga cagtacacca gactgcctgc ggacattaaa gaggcagagt tcaaaggatt
     3901 gcctgttaag gtcattagat ccaacaccgc catccagatt atgaacgctc ttgcggagat
     3961 cgcccctcct aatggtgaca ggcagataat agacaaaatg gtggaaagcg gcaggaaagc
     4021 gctaggcagg aatactgatg cttaaagttt agaagaggat gtgaatacaa taaagtggct
     4081 gggttatttt taagttttga aggcattgac ggttgtggca agagtacgca ggctgatctc
     4141 tgtttcaggg ccttgcagga aatgaacatt cctgcggtaa aaaccagaga accaggggga
     4201 gaacctgtag ctgaaaaaat tagggagatc ctcctctatt ctaaagatga gatagcacac
     4261 tcaacagaag tcctgcttta tgccgcttcc agagcccagc acgttgcaag agtcattcga
     4321 cctagcctgg aagccggcaa ggtcgtagtc tgcgacagat ttgtgcattc ttccctagct
     4381 tatcagggat acggcctcgg catcgacatc gatgccattt ggaatatcaa ttatttcgcc
     4441 atgggcggaa cgtttcccga tctggtcttc cttttcgata tcgatctgga agaagcaaga
     4501 agcaggtcga aagcacgcga aaactcctca ggcagggaga taagctcgaa taacgaaata
     4561 aacaatagag aagatcgaat tgaaaaaagg gatctggtct tctactgcaa ggtgagactg
     4621 ggattcctcg aactggcaaa aaacgacagc cgcattattg tactagatgg aagagaaccg
     4681 atcgatatcc tccacggaaa ggtgatgaaa gcagttaccc gcgccttaaa cgtttgaaga
     4741 aggtttggtt tttacgatgt caagaccaaa ggaggttggg ataatgaagc ttgttgttgc
     4801 agtggtacaa gatcaggatg ctcctctgct tctcgataaa ctgtcagaag aaaaaatcag
     4861 ggctacaaag cttgcaagta caggcggctt tcttaagcag ggcaatacga cgctcctgat
     4921 tggtgtaaac gaaaacttgg tagacaccgt tctggatatt attagaagga cctgttcgtc
     4981 caaagagcag ctgatcacac ctgtggtaag tgacttcagc cgttctgttc ctgattttat
     5041 tcctcagcct gtctcggtaa aggtaggcgg agctacggtg ttcgttctcg acgtccacca
     5101 cttcgaaaag gtgtgaggta gcattgggcg taaaaattgg acccaaaagc ggcatgatcc
     5161 gcaccgatgt tgagctgaag gaaagcacca agctcaaagt ttccaacacc gacttcttgg
     5221 acctgttaaa gagggattcc ggtaaaaata cccagcaaga actggaaaaa ctgctgcagg
     5281 agatcgatag ccttggtgcg gctcttgcag aagacttatc gctgaagacc ctgaaaaggt
     5341 acagggaagc catcaagcag ttcatctcct atgccatcaa aggatcgatt gaggttttag
     5401 atcagtcagg catcgacata tacgggtcac cgcggtctta caagatcatc agaaaagtgg
     5461 atgattcttt agaacaactt gcacaagcca ttttgaaaag gcacgaagct caaattgaaa
     5521 ttgtggcgcg tgtaagcgag attcgcggtc ttttagtcga cctaatgggt taaaacgagg
     5581 ctgcagagca gcctcatttc tgtaaggaaa gatcagtact aggcaaagag ggtagtctct
     5641 gtgtttgaaa gagttgttgg acaagaaagc agcaaaagaa tccttactgc tgctgtggaa
     5701 aacgatggca tcagccacgc gtaccttttt catggaccaa aaggtgtagg taaaaccacc
     5761 atggcagtgg aactggcaat ggttttaaac tgcgaagaaa gaaatggttg cggcgtttgc
     5821 agcagttgta ggagaatcaa ggagggaatc ttccccgacg taaagatggt tgctcccgac
     5881 ggaccttcca tcaaaatcgg cgagattcgc gagcttcaga actttatcgc gatgagtagt
     5941 tttgaaggca gattcaaagt tgggatcatc caggatgcag agaaactc
//
LOCUS       DAOSDA010000042         5923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_8533_length_5923_cov_3.247103, whole genome shotgun sequence.
ACCESSION   DAOSDA010000042 DAOSDA010000000
VERSION     DAOSDA010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8533_length_5923_cov_3.247103"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..284)
                     /locus_tag="PLW82_01755"
     CDS             complement(150..284)
                     /locus_tag="PLW82_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46029.1"
                     /translation="MPLVFYEALVLGGGIAWARMTGTGPAVLLGKTFVILSILYPLVY
                     "
     gene            760..1035
                     /locus_tag="PLW82_01760"
     CDS             760..1035
                     /locus_tag="PLW82_01760"
                     /inference="COORDINATES: protein motif:HMM:NF019488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HOJ46030.1"
                     /translation="MLKLWQALHKKLELPSELLEREPKVAFYPRQKRVVIDNLSRIVK
                     YESGEVVVSTASGNVVLTGRNLRLNVLLPDSLEVVGELHKLEVSGNG"
     gene            1028..2131
                     /locus_tag="PLW82_01765"
     CDS             1028..2131
                     /locus_tag="PLW82_01765"
                     /inference="COORDINATES: protein motif:HMM:NF018591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HOJ46031.1"
                     /translation="MAKAVFMVVGDDIEGFLSGTEGLKLTHLKRSGRALYGKCAYRDL
                     ERVRSLAQNHNLEFKVLMQTYRPIGYFVIAVLVTGLLLGFFWSSRAWTFMYNSNDEAL
                     IDEVHAYLWERGVRPGTRTDSFTFDELEDQILQSFPQLNYVSLKKDGIVLKIEMSVRP
                     PQIEEARQENLYAAYGGIVRSTSVLLGESLVKPGDVVYKGQLLVRGNPYAEGSIIGET
                     VVELKKTVDTRRFYREETREKYWNFHIRVGGLVYDFGFKKPELSEFEVWTVTERRLGW
                     AGSRRQFVEVTLTEYIKNRVSFDIITIEEATRLAEAEVEELFETLFSGCKVFDKQIET
                     ELGKLEVNAVLRARVELDLTQERLGESIDGTNP"
     gene            2103..3074
                     /locus_tag="PLW82_01770"
     CDS             2103..3074
                     /locus_tag="PLW82_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOJ46032.1"
                     /translation="MKVLTEQIHRVVSVNDINTMKILMGPADSNWRILEKNFRGRIMS
                     RGSEIHMMGAKEDIERMEEVLAYLIAKAEKQQELSEQETSVAFLTTNQKTEYEADPIQ
                     ITARGKAIRPKTPGQQRYVEAIKENEVVFAIGPAGTGKTYLAMAMAVAALKRKEVDHL
                     ILCRPAVEAGENLGFLPGDLQDKVDPYLRPLYDALYDILGAEVVKRYMEKGIIEVAPL
                     AYMRGRTLNDAFIILDEAQNTTKEQMKMFITRLGFGSKMVITGDLTQIDLPKDKYSGL
                     MHVAKLLVGIPSIEFVYLTEKDVVRNPLVQKIILAYEKLENSLDSES"
     gene            3089..5242
                     /locus_tag="PLW82_01775"
     CDS             3089..5242
                     /locus_tag="PLW82_01775"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOJ46033.1"
                     /translation="MKTEPKPKGKKSKLPSLENKSVKSDIKAFFQKLWEYLKRDETIR
                     RLLGIGTTLIMCVIFLGSVYEERLNLNVGDVVTRDIVATHEVLDYTAWEEIQREVERS
                     VSKTASEDLSNYRVEKAYLFSAEEDFQEIIQIISDNRTEEDAKARDIGMVQNLIKTQT
                     KLDVSPSVIKNLLQISEEDLNLLRTMGLETISQVMAEGVSTTTLPDAKEKVAQLFLTA
                     DVDLGFEVLTSASEVVQAVIRPNLQLDSDKVNKLIKDAVEEARASAPTIKKGQIIVRE
                     GDVLTSKDLMILDALNMVKRSWNWQRALGIVTVTLLIAGVFYYYLYSFYRNLENKYFV
                     LYCTIILVVAVLGKLLTLIDWPYAIYFFPASAATMLLTLLVNPTIGVFTAVLYSLMTA
                     FFAEFAIPPVLMSLFGAVVGVYCVKGATQRGSLMQAGLLVGVTNALSMIACGLILQET
                     SLMVMSFVGLINGIVSAVLTIGLLPYLELIFDVASPMRLLELSNPNNPLLRRLSLEAP
                     GTYHHSIVVGNLAEAAAEAIGADPLLTRVGALYHDIGKLARPYFFVENQRGENPHNKM
                     SPNLSRMVILNHVKEGLELAKEHKLPKFITDFIVEHHGTSTVQHFYEKAKETDQNPVL
                     LEDFSYPGPKPQSKETAILMLADSAEAAVRSMTQPTYDKIQSMIKSIAKAKLNDGQLS
                     RSTLTLREIEIITEVMSRQAAGIHHSRLKYPGQKL"
     gene            5251..5706
                     /gene="ybeY"
                     /locus_tag="PLW82_01780"
     CDS             5251..5706
                     /gene="ybeY"
                     /locus_tag="PLW82_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOJ46034.1"
                     /translation="MELYVENEQEKLQVEKRTIDALHKAMNIASNLHSLEDSTVNIIL
                     VDNAKIREINREYRGVDRETDVISFALNDFEVTFPWEREELGDIYISVEKALEQSREY
                     GHSFDRELVYLAVHGLLHLLGYDHLEEAEKKEMRQEEEKIMAEVGLLRE"
     gene            5731..>5923
                     /locus_tag="PLW82_01785"
     CDS             5731..>5923
                     /locus_tag="PLW82_01785"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOJ46035.1"
                     /translation="MIDLNWKKHSLYDSFNCAIRGVIQAVKMERNLRIHLLSAALAIG
                     LGVILDLSRLEFVVLFITIG"
BASE COUNT         1661 a         1336 c         1532 g         1394 t
ORIGIN      
        1 gtaggcaggt acaagtaagt ggtgcatgcg tggaacactt agtgaacagg tgcaggcaaa
       61 aaaatcaggc ctacgcatat ggccatatag actaaaggga agaccgctgc gaaagggtgt
      121 ctctattttt tgtaaaggaa aacgcatgtc tagtagacga gcgggtacag gatggaaaga
      181 atcacaaagg tcttacccaa aagaactgca ggtcccgtac ctgtcattct cgcccacgct
      241 atcccgccgc ctaaaacgag tgcttcataa aacacaagtg gcattcagga aaacaccaaa
      301 aggtaaaaag cccaaccttt gcggtctgcg gtttgcgcgt tccgcttgtc tatcaacagc
      361 accaaaacgg gcacgagcca aaggaagtgc cagacccaaa gaccgatata cagttttgtc
      421 agctcctctg cgcaccttct tggtgtccgc aaaaaacaga ttggtgtcct tccttttacc
      481 ccgatggttt ttcctgcacg accaagttcc ttcaaaacaa atagaggaga actgcagcag
      541 tgggcagtac agatgtttag gacaccgacg agggagtctt ccgcgacact cttttcactc
      601 tatttgcata aatggttttt tggaccgttg tcgaagtatt cttgtgacga ggtgagaaag
      661 tacataatag gcactgggaa aaacaggaat ccggaggatt tcgtatcttc taacttccct
      721 agatttcgaa tagatttagt atctgaggga ggggtgagga tgctcaaact ttggcaggcg
      781 ttgcacaaaa aattggaact accatcagaa cttttggaaa gagagcccaa agttgcattt
      841 tatcccaggc aaaagcgtgt tgttatagac aatctcagcc ggatcgtaaa gtacgaaagc
      901 ggcgaagtgg tagtgagcac agcttcgggg aacgtggttc tcacaggtag gaatctaagg
      961 ctgaacgttc tcctgcccga ctctttggag gtggtagggg aactccataa actagaggtg
     1021 agtggaaatg gctaaagcag tgttcatggt agtaggagat gacattgaag gcttcctttc
     1081 cgggacagaa ggcttaaaac tcacccatct caagcggagc ggcagggcgc tttacggcaa
     1141 gtgcgcctac cgcgacttgg aaagagtcag aagtcttgcc caaaaccaca atttggagtt
     1201 taaggttctc atgcagacct acagaccaat cggctacttc gtgatagctg ttcttgtcac
     1261 cggacttctc ctaggctttt tctggtctag tagggcttgg acattcatgt ataatagtaa
     1321 tgatgaagcc ctgatcgatg aggtacatgc ctatctttgg gaaaggggag tccgtcccgg
     1381 aaccaggacc gacagtttca ctttcgatga gttggaagac caaatccttc agtctttccc
     1441 gcagttgaac tacgtcagcc tgaaaaagga cggcattgtg ctgaaaatcg aaatgagtgt
     1501 gaggccgccg cagatagagg aggctcggca ggaaaacctc tatgcggcat acggtggtat
     1561 tgtgcgctct actagcgttc ttttaggcga aagcctggtt aagccgggcg atgtggtcta
     1621 taaaggccag cttcttgtga gaggtaaccc ttatgccgaa ggcagtatca tcggggagac
     1681 agtggtagag ttaaaaaaga ccgtagacac ccgtcggttt taccgagaag aaaccagaga
     1741 aaaatactgg aacttccaca ttagagtcgg cggtctcgtc tacgacttcg gcttcaaaaa
     1801 gcccgagctc agcgagtttg aggtttggac cgtaaccgag cgccgtttgg ggtgggcagg
     1861 aagcaggaga cagtttgtcg aagtaacttt aaccgaatat atcaaaaatc gtgtctcctt
     1921 tgacataatt actatagaag aggcgacccg ccttgcggaa gctgaggtcg aggaattgtt
     1981 cgagaccctt ttctctgggt gcaaggtttt tgacaagcag atagaaacgg agctcggcaa
     2041 actcgaagtt aacgcagttt tacgggcaag agtcgagtta gatttgacac aggaaaggct
     2101 cggtgaaagt attgacggaa caaatccata gagttgtatc tgttaatgat ataaacacca
     2161 tgaaaatcct catgggtcca gcagactcca attggaggat cctggagaaa aacttcagag
     2221 gcagaatcat gtctcggggc agcgagatcc acatgatggg tgccaaagaa gatatagaaa
     2281 gaatggaaga ggtccttgct tacctcatag ccaaagctga aaagcagcag gaactcagcg
     2341 aacaagagac gtctgtagcg tttcttacca ccaatcaaaa aactgagtat gaggcagacc
     2401 ccattcagat taccgctcgg ggaaaggcca tcaggccgaa gactccagga cagcagagat
     2461 acgttgaggc cattaaggaa aacgaagtgg tttttgccat cgggcctgct ggaacaggta
     2521 agacctattt ggctatggcc atggctgtag ctgccttaaa gcgcaaagag gtcgatcatt
     2581 taatcctttg cagacctgct gtggaagcag gcgagaatct cggctttctg ccgggagatc
     2641 tccaagacaa ggtcgatccc tacctcagac ctctctacga tgccctgtac gatattctcg
     2701 gagcggaagt ggtcaagcgc tatatggaaa aaggaatcat cgaagtggct ccgctggctt
     2761 acatgcgggg caggacctta aacgatgcct tcatcatact ggatgaagct cagaacacta
     2821 ctaaagaaca gatgaagatg ttcatcacca gactgggttt tggttcaaaa atggtgatta
     2881 ccggagacct aacccagatc gacctgccaa aggacaagta ctctggtctc atgcatgtag
     2941 caaaactgct cgtaggtatt ccgagcattg agtttgtcta cctaaccgaa aaagacgttg
     3001 tgcgcaatcc gttagtgcaa aagataatcc tagcctatga gaaattggaa aattctctgg
     3061 acagcgaaag ctgagtgggg aggagactat gaaaactgaa cctaaaccta aaggcaaaaa
     3121 gtcgaagttg ccgtctttag agaataagag cgttaaaagc gatatcaaag cgtttttcca
     3181 gaagctatgg gagtacttaa aacgcgatga gaccatcagg cgtcttttag gcatcggaac
     3241 aaccctgatt atgtgcgtga tctttctcgg atctgtttac gaagaaagac ttaacctcaa
     3301 tgttggcgat gtggtgacgc gagatatcgt tgccacccac gaggtactcg actataccgc
     3361 ttgggaagag atccagcggg aagtcgagcg cagcgtatcc aaaaccgcaa gtgaagacct
     3421 gtccaactat agggtggaga aggcgtatct cttttctgca gaagaggact tccaggagat
     3481 cattcagatt atcagcgaca acagaacaga agaagatgcc aaagccagag acattggcat
     3541 ggtgcagaac ttgatcaaga ctcagacaaa actggatgtc tctccgtcgg tgatcaagaa
     3601 ccttctgcag atctccgaag aggacttaaa cctcctgcgc accatggggc ttgaaactat
     3661 cagccaggtg atggcagagg gtgtatccac tactacgctg cctgatgcca aggagaaggt
     3721 tgcacagctc tttttaacag cagacgtgga tctggggttt gaagttctga ccagcgcatc
     3781 agaggtagtt caagcggtga tcaggccaaa cctccagcta gacagcgata aagtaaacaa
     3841 gctcattaaa gatgctgtag aggaagcccg cgccagtgca cctaccatca agaagggtca
     3901 gatcatcgtt agagaaggcg acgtgcttac cagtaaagac ctgatgattc ttgacgcatt
     3961 gaacatggta aagcgcagct ggaactggca gagggcattg ggaatagtca ctgtcacact
     4021 gcttatcgcc ggcgttttct attattactt atactctttt tatcggaact tagaaaataa
     4081 gtattttgtt ctctactgta cgatcatttt ggttgttgcc gtgttgggta agctccttac
     4141 actgatcgat tggccgtatg ctatctactt cttcccggca tcggcagcca caatgcttct
     4201 tactcttcta gtgaatccaa caatcggcgt gtttacagct gttttgtact cgctgatgac
     4261 ggcgttcttt gcagagtttg ccatacctcc ggtgctcatg agcttgtttg gggccgtggt
     4321 tggtgtctac tgcgtcaagg gcgcaaccca aagaggcagt ctaatgcagg caggcctgct
     4381 tgtgggcgtt accaacgctc tttcgatgat cgcctgcggt ttgatactgc aggagaccag
     4441 cctcatggtg atgagctttg tgggtcttat caacggcatc gtctctgccg ttctgaccat
     4501 cgggcttttg ccttacttgg agctcatctt cgacgttgct tcgccgatga ggcttttgga
     4561 gctttctaat cccaacaatc cgctccttag aaggctttca ctggaggcac cgggtaccta
     4621 tcatcattcg attgttgtag gcaacttggc ggaggcggca gctgaagcca tcggtgctga
     4681 cccgctcctc accagagtgg gtgcattgta ccacgacatc ggcaagctcg cgcgtcccta
     4741 cttttttgtg gaaaaccaaa gaggggagaa cccccacaac aaaatgtcac cgaacctttc
     4801 acgcatggtg attttaaacc acgtcaaaga aggtttggaa ctggctaagg agcataaact
     4861 gccgaagttc atcaccgact tcatcgtaga acatcacggt acatctacag tacagcactt
     4921 ctatgagaag gcgaaagaaa ccgatcagaa tcccgtgctg ctcgaagact tcagctatcc
     4981 cgggcccaag cctcagtcca aggagactgc cattctcatg ctggcagact cagctgaagc
     5041 ggcagttcgt tccatgactc agccaactta cgacaagatc caatcgatga tcaaaagcat
     5101 tgccaaagct aaactcaacg acggtcagct cagcagatcg acgctcactc taagagaaat
     5161 cgaaattatc accgaggtaa tgagccgtca agcggcaggt atccaccaca gcaggctcaa
     5221 gtacccaggt cagaagctgt agggattaaa atggaactgt atgttgaaaa cgagcaggaa
     5281 aaactgcaag ttgaaaagcg aactatagat gcgttgcaca aggctatgaa tattgcatcc
     5341 aatcttcata gccttgaaga ttccacggtg aacattatac tggtagataa cgccaagatt
     5401 agggagatca atagagagta caggggagta gaccgggaaa ctgacgtgat ctcgtttgcc
     5461 ctgaacgact ttgaagtgac ttttccctgg gaaagggaag agcttgggga catctacatc
     5521 tccgtagaga aggctttaga acaaagccga gaatacggcc actcttttga cagggagctg
     5581 gtgtaccttg cggttcacgg ccttctccat cttttagggt acgatcatct ggaagaagcg
     5641 gagaaaaagg aaatgcgaca ggaagaagaa aaaatcatgg cggaagtagg gcttctgcgg
     5701 gagtagattg cctatcccga acctaaggta gtgatcgact tgaattggaa aaagcactct
     5761 ttatacgaca gctttaactg cgctattcgc ggtgtaatac aggcggtcaa gatggaacgc
     5821 aacctcagga tccatctgct ctctgcggct ttggccatag gtttgggcgt gatccttgac
     5881 ctgtcgagac ttgagtttgt cgtcttgttc atcactatcg gta
//
LOCUS       DAOSDA010000043         5914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_8542_length_5914_cov_2.871138, whole genome shotgun sequence.
ACCESSION   DAOSDA010000043 DAOSDA010000000
VERSION     DAOSDA010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8542_length_5914_cov_2.871138"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1355)
                     /locus_tag="PLW82_01790"
     CDS             complement(72..1355)
                     /locus_tag="PLW82_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46036.1"
                     /translation="MKGKYKWLLCLILASFLVTAVSAQKITVWTWYDAGLGQILRDLV
                     KNEFTPRTGIEVEIVTVPIGDMTNKLLLAYLGGDAPDVVELYSNTVVDLGIRGALLNL
                     NTFPDVKEVKADFNPMLLPALQYKSALYAIPGEVNWTWTYYRADILSDIGVEAPVTWD
                     DVKELSIKLKAREMDIYYYYQGDPSALIVGKYLPFSFQRGTDIYSADGTASTLDSPEN
                     IEAFKELTSLHTDYKLPLEDTIYTTFVNGKTPVQILQNWYYHAFEVTAPHLAGMWNIS
                     LFPGTKQRDGSIDHTNTGKMLVWSIVSSSKQKEAAWEFVKFINSSETTVKWMNRAYES
                     NDKARLFFSNMNSIEHAVFPEEHIALAITALETCRMQTAVIGGNIANRYVDFAFNKVV
                     LQGDDPEAAIKQAAKESTLEIQKKLKEFDRFIKDL"
     gene            1668..2576
                     /locus_tag="PLW82_01795"
     CDS             1668..2576
                     /locus_tag="PLW82_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46037.1"
                     /translation="MRKVIAASSVYKSYRKDPVIKDLSFTVSEGEIVGLLGPNGCGKT
                     TTVRLLNGVISSDQGSIEVFGLKPEESGEDIRRLSGVLTESAGLYPHMSGRANLEFFA
                     DLYGVEDKGRVDQLLKDFGLFEHKDKKVGSYSTGMKKRLGIAKALLHNPKLLFLDEPT
                     NGLDPEGARDLLVYIKKLNEEKGVTIFLCTHILNHAENLCDRFLFMADGTIIEEGTLA
                     EVEARYLKEFLVEVETDIAEEKLPEELKASFSSRDGNKLVFKVASKEHIPQLLAKLLE
                     VGPVYTSRILGRDLETMYFKIREAKR"
     gene            2573..3406
                     /locus_tag="PLW82_01800"
     CDS             2573..3406
                     /locus_tag="PLW82_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ46038.1"
                     /translation="MNRKAVLAIAKKDITSITSSVQLFLPIIVVPLIILILVPGGLLI
                     AARYVDVTETTNMGPILKLLDSLPAGNLKTALDSFTDINQKMLFLFLNYFFAPLFLLI
                     PLMAASIVSANSFAGEKERRTLESLLFAPVDIFSLFLGKVLAAFALSMVMAVVGFILY
                     GLVVNGLGYAIFHRLIFPEPNWWVLIFFVAPSFSFLAILFNVIVSAKVKGFQEANQMS
                     VFVILPVLALVISQITGALLISSKVLWVIGIVTLLLSVFVLRQIVKHFDRNKLFESQI
                     F"
     gene            3462..3932
                     /locus_tag="PLW82_01805"
     CDS             3462..3932
                     /locus_tag="PLW82_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HOJ46039.1"
                     /translation="MLYTCPYGRLGAVRKGIKPAELVKLDGLCLRCLKETDPDLHFFD
                     LGRTFYLVYGDLLEETLKSPCAREVLLSLGYGKDLEACLAALRERFAAGCPPEIGFFL
                     GYPVKDVLGFMGIPGFTFAKTCGWQMHNPVEASEVVYRRYTKAKYRALLPKCRV"
     gene            complement(3977..4828)
                     /locus_tag="PLW82_01810"
     CDS             complement(3977..4828)
                     /locus_tag="PLW82_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46040.1"
                     /translation="MKRLIVAFVSGSLKTGRSTIAANLANYLRKTTSVTLLDSDIEQV
                     ERAVLLPPEWSAGSEITVLFPSHDLSKCQHCLQCTTICEAGVFKDYKEKIILLPELCN
                     NCGKCLEYCKSQAVKFTEYTIGRIQEAKLDNLTVIEVRVNDEIVKASSLFYHTKDYLN
                     PLDVILVDAAPLISEYACCSTMGADYFVIVTGSGESHLAQLKETIKVFKDYEKPYGVL
                     VNLSDSEVSPTAEYCRENDIPLLGVLPLDEKLQTGKTPRDKLADEPEWQKLFESLWHR
                     ILEEIAQ"
     gene            complement(4839..5612)
                     /locus_tag="PLW82_01815"
     CDS             complement(4839..5612)
                     /locus_tag="PLW82_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOJ46041.1"
                     /translation="MQKLELNKDSSIKYVIAVMSGKGGVGKSSVSGLLAASLQKKGYK
                     VGVLDADITGPSIPKIFGVTENLLSVGGKVYPAETDLGVKVVSVNLILESEDDPVIWR
                     APLLNSTISQFWTDITWGELDYLIVDLPPGTADVSLTVLGMLPVTGVVMVSSPQELAQ
                     MVVRKALKMAAKMSVPLLGLVENMSYAICPHCGEKHEIFGPSKGRQLAEMFDIRFLGS
                     LPLDPKLTELADQGRIESYTLPNDLDLATLVDRAVRTLA"
     gene            complement(5724..>5914)
                     /locus_tag="PLW82_01820"
     CDS             complement(5724..>5914)
                     /locus_tag="PLW82_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46042.1"
                     /translation="LVGRVDFKDDPLALEADAELIDQYFDVFVPTCIYHPKKMEHIHS
                     LLGEKVNYLFIGETIEVY"
BASE COUNT         1460 a         1452 c         1471 g         1531 t
ORIGIN      
        1 cctcaaagaa cgagacacgc tagacttcta acaaaaaaga ggaagggcgc gcccttcctc
       61 tcaacgttac cttatagatc tttaatgaag cggtcgaact ccttcagttt cttctggatt
      121 tctagagtgg actccttggc agcttgcttg atggctgctt ctggatcgtc gccttggaga
      181 acaaccttgt taaaggcgaa gtcgacgtag cggttggcaa tgtttccgcc aataactgca
      241 gtctgcatac ggcaggtctc cagggctgtt atagccaggg caatgtgctc ttctgggaac
      301 accgcatgct caatggagtt catgttgctg aagaacagcc ttgctttatc gttgctttcg
      361 taagcacggt tcatccactt gacggtggtc tccgacgagt tgatgaactt cacgaactcc
      421 caagctgcct ctttctgctt agaagaggag acgatggacc agaccagcat cttaccggtg
      481 ttggtgtggt cgatagaacc gtcccgctgc ttggtgcctg ggaagaggga gatgttccac
      541 attcctgcaa ggtgaggtgc tgtaacctca aaggcatggt agtaccagtt ctggagtatt
      601 tgtacaggag tcttgccgtt aacaaacgtc gtgtagatcg tgtcctcaag cggcagtttg
      661 tagtcggtgt ggagcgatgt cagctctttg aaagcttcga tgttctcagg agaatccaga
      721 gttgaagctg tgccgtctgc tgagtagatg tcggtgcccc tctggaaaga gaacggcagg
      781 tacttgccga cgataagggc agatggatcg ccctgataat agtagtagat gtccatttca
      841 cgggctttca gcttaatgct gagctctttc acatcatccc aggttacagg tgcctctaca
      901 ccgatatcgc tcaggatatc tgcacggtag taggtccagg tccagttgac ctcaccggga
      961 atagcgtaca gtgccgactt gtactgcaga gcaggaagca gcatagggtt gaagtcagcc
     1021 ttgacttctt tgacatctgg aaaggtgttc agattgagca gcgctccccg gatgcccaag
     1081 tcaactacgg tgttggagta gagctccaca acgtcaggag catctccacc gagatatgca
     1141 agtaaaagct tgttggtcat atcaccaata ggtacggtga cgatttcaac ttcgattccg
     1201 gtccgtggag taaactcatt tttaaccaga tctctcagga tttgtccgag tcctgcgtca
     1261 taccaggtcc agacggtaat cttttgggca gaaacagcag tcacaagaaa tgaagcaaga
     1321 ataagacaga gaagccattt gtactttcct ttcacctttt tctcccccta tcgccgattt
     1381 tttgtgataa tataagtagc taggttcttt tctagctcct tacaccgaaa aaagagacaa
     1441 agaccgtact cccactggaa ggatctggct taactagaat ctttaccctg ttccagcgga
     1501 gtgcccaaag tgcgcgtttt aaactcttca cgcgcacagc caccctataa caatatttag
     1561 gctttgcgta aaaaatcctg ctttaaagcc ttcggtcaag agaagtcttt cggtgcagtg
     1621 agctggaaaa agcctgtaga aaacagcaaa cacaggaggt gcagcgattg agaaaagtaa
     1681 tagcagcgtc tagtgtctac aaatcgtatc ggaaagatcc ggtaatcaag gacttgagct
     1741 tcacagtgag tgagggagaa atcgtcggtt tactcggacc aaacggctgc ggtaagacca
     1801 caacagttag actcctaaac ggcgtgatct cgtcggacca ggggagcatt gaagtcttcg
     1861 gcctgaagcc cgaagaaagc ggagaagaca ttaggcgtct ttctggagtt ctcacagaaa
     1921 gcgccggctt atacccccac atgagcggca gagctaacct ggaatttttt gccgacctct
     1981 acggcgttga ggacaaaggc agagtagatc agctcctcaa ggatttcggg cttttcgagc
     2041 acaaagataa gaaagtgggc agctattcta ctggcatgaa aaagagactc ggcattgcca
     2101 aagccttact tcacaacccc aaactcctct ttctcgatga gcccacaaac ggtttggatc
     2161 cagaaggtgc cagagacctt ttggtgtaca tcaaaaagct caatgaggag aagggagtca
     2221 ccatctttct ctgcacccac attttgaacc acgccgaaaa cctctgcgac agattcctct
     2281 tcatggcaga cggaaccatc attgaagagg gtactctagc agaggtagaa gcccgctatc
     2341 tgaaggaatt cctggttgag gttgagacgg acatcgcaga agagaagctc cctgaggagc
     2401 tgaaagcgag cttctccagt agagatggaa acaaactggt cttcaaggtg gcaagtaaag
     2461 agcacattcc ccagctcctt gcaaaactcc tggaggtagg tccggtatac acttccagga
     2521 ttctcggtcg tgacctggag accatgtact tcaagataag ggaggcgaaa agatgaatag
     2581 gaaagcagtt ttggccatag ccaaaaagga cataacttcc ataacttcaa gcgtgcagct
     2641 ctttctcccg attatcgtag tgcctctgat catcttgatt ttggttcccg gaggactcct
     2701 catcgccgcg cgctacgtgg acgtgacaga gaccaccaac atgggaccga ttctaaagct
     2761 tctagacagc ctccctgcag ggaacctgaa gaccgcttta gatagcttca ccgatatcaa
     2821 tcagaagatg ctattcctct ttctgaacta tttctttgcc ccgctgtttc tcctcattcc
     2881 cctcatggca gcaagtatcg tctcggcaaa cagctttgct ggagaaaaag aaaggcgcac
     2941 tttggagagc ctcctctttg cgccggtaga tatcttctct ctgtttctag gaaaggtttt
     3001 ggccgctttt gccctgtcaa tggtcatggc agtggtggga ttcatccttt acggcttagt
     3061 ggtaaacggt ctgggttatg ctatcttcca ccggctgatc ttccccgagc ctaactggtg
     3121 ggtgctgatc ttcttcgtcg ctcctagctt cagctttctg gcaattctct ttaacgtgat
     3181 cgtctcagct aaggtcaagg gttttcagga agcaaaccag atgagcgttt tcgtgatcct
     3241 accggtactt gccctggtca tctcccagat tacaggagcg ctgctcatta gctccaaagt
     3301 tttgtgggtg atcggcatcg tcacgctgct tttgtcagtc tttgtgctca ggcaaatagt
     3361 gaagcacttt gaccgcaaca aactcttcga aagccagatc ttctaactta cgacacctgc
     3421 attagttcct tcaagatggc tactctaaac aaggggtgcc tatgctatac acctgtccgt
     3481 acggtaggct tggggcagtg cggaagggca ttaagcctgc agaactggta aagttagatg
     3541 ggctttgcct ccgctgcctt aaagagaccg atccagatct acacttcttt gatctgggga
     3601 gaacgttcta tcttgtctac ggagatcttc tggaagagac cttgaaaagc ccctgcgcta
     3661 gggaggttct gctttctcta ggctacggga aagacctcga ggcatgtctt gccgctctcc
     3721 gggagaggtt tgccgcaggc tgccctccgg agatcggctt tttccttggc tatccggtta
     3781 aagacgtctt gggatttatg ggcattccgg gatttacctt tgcgaaaacg tgcggttggc
     3841 agatgcataa cccggtagag gcctctgagg tcgtctacag acgctacacc aaagccaaat
     3901 acagagcact gctccctaag tgcagggtat aaaaagagaa ctgcgacggc agttcttttt
     3961 ttgtgcagcg tgctgcttat tgagctattt cctctagtat ccgatgccag aggctttcaa
     4021 aaagcttttg ccattcgggc tcatctgcga gtttgtcgcg gggagtcttt cccgtttgca
     4081 gcttttcgtc gagtgggaga acccctagaa gaggtatgtc gttttccctg cagtactctg
     4141 ctgttgggct gacctctgaa tccgaaaggt taactaagac gccgtagggt ttttcgtaat
     4201 ctttgaaaac cttaatggtt tctttgagct gcgcaagatg gctctcgccg cttcctgtga
     4261 cgataacgaa gtagtctgcg cccatggtgg agcagcaggc atactcagat atgagaggcg
     4321 cggcgtccac taagatcaca tctaaaggat tcaagtaatc tttcgtgtgg tagaagagag
     4381 agctagcctt tacgatctca tcgttcaccc tcacttcgat cacagtgaga ttgtccagtt
     4441 tggcttcttg gattctgcca atagtgtact cggtgaactt caccgcttgg ctcttgcagt
     4501 actctaagca ctttccgcag ttgttgcaca gttcaggaag gagaatgatc ttctccttgt
     4561 aatccttgaa aacaccggct tcgcaaatgg tcgtgcactg caagcagtgc tggcatttgc
     4621 tgagatcatg agatggaaaa agcacggtga tctcgctgcc tgcagaccac tcagggggaa
     4681 gaagcacggc tctttcaact tgctcgatat ccgaatccaa caaggtgaca gaggtagtct
     4741 ttcttaggta gtttgctaga ttcgccgcta tggttgagcg gccggttttg agacttccgc
     4801 ttacaaaagc cacaatcaac cgtttcacat ccttcggact acgccagagt tctcacagcc
     4861 ctgtcgacaa gagttgcaag atccaggtcg tttggtaatg tataggactc aatcctgcct
     4921 tgatctgcaa gttcggtgag cttggggtca aggggtagag agcctaggaa cctgatatca
     4981 aacatttcgg cgagttgcct gcctttggaa ggtccaaaga tttcgtgctt ttcgccgcag
     5041 tgtggacaga tggcatagct catgttctca acaagtccaa gtagaggaac actcattttg
     5101 gcagccattt tcaaagcctt gcgcacaacc atctgtgcca gctcttgagg tgaggaaacc
     5161 atcaccacac cggtaacagg cagcatacct aaaaccgtaa gcgaaacgtc ggccgttcct
     5221 ggagggaggt ctacgatgag gtagtccagc tcgccccagg tgatgtcggt ccaaaactga
     5281 gaaatggtgc tgtttagaag cggtgccctc catataacgg gatcgtcttc actttcgaga
     5341 atcaggttca ccgacaccac tttgactccc agatctgtct ctgctgggta gactttgccc
     5401 cctacagaaa gcaggttttc ggttacaccg aagatcttgg gaatggaagg tccggtaata
     5461 tcggcatcta aaacgccgac cttataacct tttttctgga gcgatgccgc aagaaggccg
     5521 cttacagagg attttccaac gccgcccttg ccgctcatca cagcgattac atacttgata
     5581 ctgctgtctt tgttcagctc taacttttgc attgtttggg ctgtgcccat cacctcgatt
     5641 gtaattagct agattctcca ggggcaaaaa aatctccgcc caaatactaa tctcaacaga
     5701 agagtaaatg cgcgatccct ttatcaatat acctcaatcg tttcgccaat aaacaagtag
     5761 ttaacctttt cgcccagcaa gctgtggatg tgctccattt tcttcggatg gtagatgcag
     5821 gtaggtacga acacgtcaaa gtactgatcg atcagctcag catcggcttc taaggctagg
     5881 ggatcgtctt tgaagtcgac ccttcccacc aaca
//
LOCUS       DAOSDA010000044         5906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_8552_length_5906_cov_3.646043, whole genome shotgun sequence.
ACCESSION   DAOSDA010000044 DAOSDA010000000
VERSION     DAOSDA010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8552_length_5906_cov_3.646043"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /gene="dnaE"
                     /locus_tag="PLW82_01825"
     CDS             complement(<1..1495)
                     /gene="dnaE"
                     /locus_tag="PLW82_01825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOJ46043.1"
                     /translation="MFVHTHVHTDGSLQDGLSTVEEIIKKAAEDKQPAVAITDHGNMF
                     RETKAHKLGKEYGVKVISGCELYLSRGKKGEKVPKPYHLVALAQNEKGYKNLIRLVSL
                     AQQQFYYRPQIYWEDLEEFHEGIIFQSACLGGTEPQLILAGLREEAKQEALRRDELFG
                     RGNYYLELQDHGMPEQLIVNRELLEISKATGIPVVASNDSHFTERDQAELHRVLVCLQ
                     WGITLDEYAVRAEESFSNEHYFKTAREMQEIFREVPQAIKNTLVIAEKCEFEMKFGEL
                     HLPEFPLEGGSIHEYFRRQVQLGLEQRYGKNPSREVLERTEFEMKVIEQMGYESYFLI
                     VSDFINWAKKNGIPVGPGRGSAAGSIVAYLLGITEVDPLVYNLLFERFLNPDRIGMPD
                     IDVDICQRRRPEVINYVKSKYGEDHVGQIITFGTFGARGVVRDIVRVLGLPYSLGDKI
                     AKSIPAALGMTIDKALEGELGQLCKDDPEIDRVVRYAKGLEGHARHAS"
     gene            complement(1700..3334)
                     /locus_tag="PLW82_01830"
     CDS             complement(1700..3334)
                     /locus_tag="PLW82_01830"
                     /inference="COORDINATES: protein motif:HMM:NF027473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4855 domain-containing protein"
                     /protein_id="HOJ46044.1"
                     /translation="MLKLVVVAIMLLTSIVSANEAFEFQGFDAWYLQQKDWVKTDNTL
                     SWPHVSWAEARLGEFQWTDYDLTFTVVPEEYGKDGEVRLYFRQTKPFHTYGLHIREDV
                     IFVSRFDSTVENYRILEQADGVGVKQGEEAKIRLVCKESDFYVYLNDELILEIYSIKY
                     KSGGIAFWAENTKFTVKDFSITGVRDETKVSTDMGFFPQNDPRSGGVNHMMLIYNNSG
                     YNWMMIDALPYVSYIAPLMGNYELKVEDYFFDTFLFLALTAPDGHAFDSPSRGTPAKK
                     EQWQWFIDKIFHENNQLSAFEKAYEFTNDALGGDVKGKIFIMIPNPMVEIKDFGDVDG
                     TGSLNFSWKDAEKAKEHRFKAVKWYIDEVLARWEAANYQNLELLGFYWVEEWIHYSNP
                     GELELIQMTSDYIHELGYKHTWIPYFTSSGHNRWEEAGFDFIMHQPNHMFRADSTIDR
                     FVHTTNHALKYGQGLEIEAEGNSVTRDPVKRKKFLDYLRAGVTLNWQKEAVHGYYQGV
                     NYFGSCAISDDPKAREIYDLVYQYVKGIFDEPLGTK"
     gene            complement(3457..5157)
                     /locus_tag="PLW82_01835"
     CDS             complement(3457..5157)
                     /locus_tag="PLW82_01835"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOJ46045.1"
                     /translation="MDLVKTSPRDPLNSEISISELGLSTRAVNALMSVGLDTLEKVSS
                     KTYEELLKIPRIGQVTAAEITRKVHRTMRDTGKPTKGSLAAIMDLDLLVLALDRRAFR
                     SLTNAGISTVGELIALDKKTLLTFKGIGEKTCELIDRALQHFLKQREEGDIAIANLGL
                     DNRTLNALSKNGIFYVSQIANMSREELASLSGIGTTSLNNLLTRYTLVTGKTIASSRE
                     KYKLDHTCESKTPLEKLDLPSRAYNALKNSGFSFVEEIINLKDSDFLSIKNIGERSLK
                     AILHELERFNGRGSAMREVKSLDDCIYLLETVLSDRQRDILNRRLAIWAKYPETLESI
                     AKDYGLSRERVRQIEKKARKKLFAASNIKTISPLLEELRGILRSFGGVCSINYIAAEL
                     LKKFKARRANTVTVIPLFLGLAGCRELSEGLWALDGITKDQIDAICERAESIIVEKGH
                     LDLDDLVDSIATAFAETEKSLIRAVVASSKKLVVAANGKVQLSERILASKSRVDYAKV
                     VLRRLKRPAHYREITELINEMLPEKNRYSPKSIQVALGSSKDMVRVQVGTYALREDVN
                     "
     gene            5623..5844
                     /locus_tag="PLW82_01840"
     CDS             5623..5844
                     /locus_tag="PLW82_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOJ46046.1"
                     /translation="MKEKVVAILDRIRPSLQADGGDVELVDVSDDGIVKVRLVGACAG
                     CPMSTLTLKNGIERILKENVPEVQSVLAV"
BASE COUNT         1413 a         1449 c         1303 g         1741 t
ORIGIN      
        1 gtgatgcatg ccttgcatga ccttccagac ctttagcgta acgtactacg cgatcgatct
       61 ctggatcatc cttgcaaagc tggcccaact cgccttctaa agctttgtcg atagtcatac
      121 ctaaagcggc tggaatcgac ttggcgatct tgtcgcccaa actgtaagga agacctaaaa
      181 cccttacgat gtcgcgaaca acgcctcttg caccaaatgt tccaaaagta atgatctgac
      241 ctacatggtc ctctccgtat ttgcttttga catagttaat aacttccggt cttcttctct
      301 gacagatatc gacgtctatg tcaggcatgc caatgcgatc gggattaagg aagcgttcaa
      361 agagaaggtt gtaaaccaaa gggtcgactt cagttatgcc caacagataa gctacgatag
      421 aaccagctgc tgagcctctt cctgggccta caggaatgcc gtttttcttg gcccagttga
      481 tgaagtcgct gacgatcaga aagtacgatt cgtaacccat ctgctcgatg accttcatct
      541 cgaactcggt gcgctcaagc acttccctac tggggttttt accgtagcgc tgttccagac
      601 caagttgaac ctgtcttctg aagtactcgt ggatgcttcc gccctccaga gggaactccg
      661 gcaggtgcaa ctcgccgaac ttcatctcga actcgcattt ttccgctata accaaagtat
      721 ttttgatggc ttggggtact tccctgaaga tctcttgcat ttccctagca gtcttgaagt
      781 aatgttcgtt ggagaaagat tcctctgctc ttaccgcata ttcgtctagg gtaatacccc
      841 actgcaggca aaccagtacc ctgtggagtt ccgcttgatc tctctcagtg aagtgcgaat
      901 cgttagatgc aacaacagga atgccggtag ccttactgat ttccaggagc tctctgttaa
      961 ctatcagctg ctctggcatg ccgtggtcct gcagctcgag atagtagttg ccacggccga
     1021 aaagctcatc tcgcctaagg gcttcctgct tggcttcttc cctgagacct gccaaaatca
     1081 actggggttc ggtgccgccg agacacgccg actggaagat tatcccttcg tggaactctt
     1141 ctagatcctc ccaatagatc tggggtctgt agtagaactg ctgttgtgca aggctaacta
     1201 gcctaatcaa attcttatag cccttctcat tttgggctaa agcaaccaaa tgatagggtt
     1261 tggggacctt ctcccctttt ttacctctag ataagtaaag ctcgcatcca ctaatcacct
     1321 ttactccata ctctttgccc agcttgtggg ctttggtttc gcggaacatg tttccgtgat
     1381 ctgtgatagc cacagctggt tgcttgtctt ccgctgcctt ttttatgatc tcctcgacag
     1441 tactcagtcc gtcctgaagg ctgccgtctg tatgcacgtg tgtgtgaacg aacatcctga
     1501 tcccgccttt tcgagctttc cttaatacat tcccattatc gtttcgtctg aaaaacccat
     1561 ttcagttctt tcaatcgcag cgaagtagaa tgtaacaaac atcctactgg caaacttatt
     1621 ctccttttac acccgattcg ccttcaacat ctaaaacagt gccgcatcaa aaaagagccc
     1681 atgcgggctc tttttgcgct tatttcgttc ctagcggttc gtcaaatatt cctttaacat
     1741 actggtagac caggtcgtag atctctctag ccttcggatc gtctgagatc gcgcaggaac
     1801 cgaagtagtt tacgccttgg tagtagccgt ggacggcttc tttctgccag ttgagggtga
     1861 cgcctgctcg aaggtaatca aggaacttct ttctctttac cggatctctg gtgacgctgt
     1921 tgccttcagc ctcgatctcg agaccctgtc cgtacttcaa cgcgtggttt gtggtgtgta
     1981 caaacctgtc gatggtagag tcggctctga acatgtggtt tggctgatgc atgatgaaat
     2041 caaagcctgc ttcttcccaa cggttgtgac cggaagatgt gaagtacggg atccaggtgt
     2101 gcttgtaacc tagttcatgg atataatcgg aggtcatttg gatcaactct aactcgcctg
     2161 gattgctgta gtgaatccac tcttcaaccc agtagaagcc cagaagttct aagttctggt
     2221 agttagcagc ttcccatctt gcaagaacct cgtcgatata ccacttaact gctttaaagc
     2281 ggtgttcttt ggctttctcc gcatctttcc aggagaagtt gagggaacca gttccgtcga
     2341 catcgccgaa atccttgatc tcaaccatcg gattggggat catgatgaag atcttaccct
     2401 taacgtctcc gcctaaagca tcgttggtaa actcatatgc tttttcgaaa gccgaaagct
     2461 gattgttctc atggaagatc ttgtctatga accactgcca ctgttctttt ttggctgggg
     2521 tacctctaga cggagagtcg aacgcatgac catctggtgc tgttagcgcc aagaacagga
     2581 atgtgtcgaa gaagtagtct tccactttca gctcgtagtt gcccatcaac ggtgcgatgt
     2641 aggaaacgta tggaagtgca tcgatcatca tccagttgta tccgctgtta ttgtagatga
     2701 gcatcatgtg attgacgccg ccactgcggg gatcgttctg agggaagaat cccatatcag
     2761 tagagacttt ggtttcatcc cttacacctg ttatggagaa gtccttaacg gtgaacttag
     2821 tgttttctgc ccagaaagcg ataccgccac ttttgtactt gatgctataa atctcaagaa
     2881 tgagttcatc gttcaaatac acataaaaat cgctttcctt acaaacaagc ctgatctttg
     2941 cttcttcgcc ctgctttact ccgacaccat cggcttgttc cagaattctg tagttctcta
     3001 cagttgaatc gaaacggctc acgaaaatga cgtcttctct gatatgcaga ccgtatgtgt
     3061 gaaacggctt tgtctgtctg aagtacagtc tgacctcgcc atccttaccg tactcttctg
     3121 gtactacggt aaaggttaga tcatagtctg tccactggaa ctctcccaat ctggcttctg
     3181 cccagctgac atggggccag gaaagagtat tgtctgtttt gacccagtct ttctgctgca
     3241 aataccatgc gtcaaaccct tgaaactcga atgcttcgtt ggcgctgacg atgcttgtga
     3301 gtagcattat tgcaacaacc actagtttta acaaacttat ccaccacctc aacagttttt
     3361 cttcaacaac tttctccact cacctgaaat ttacctttag agaatggtaa aaaagtagga
     3421 ataagagaga acctcctatt cctggaaata cgttatttaa ttaacatctt ctctgagagc
     3481 ataggtgcca acctggaccc ttaccatgtc cttcgacgag cccaacgcta cttggataga
     3541 tttcggcgag taacgattct tctctggcag catttcgttg atcagctcgg tgatttccct
     3601 gtagtgcgct ggacgtttca accttctgag taccactttg gcgtagtcca ctcggctttt
     3661 tgaagcgagt attctctccg aaagctgcac tttaccgttt gctgcgacta ccagtttttt
     3721 agaactggca acaaccgccc tgatcaaact cttttcggtc tcggcaaatg ctgttgcgat
     3781 cgagtccacc agatcatcta ggtcaagatg tcctttctca acaatgatag actccgctct
     3841 ctcgcatatg gcatctatct gatctttggt tatcccgtcc aaagcccaga gtccttctga
     3901 cagttctctg caacctgcta gaccaaggaa aagcggaatc acggtcacgg tgttcgcccg
     3961 tcttgctttg aatttcttta agagctctgc agcaatgtaa ttaatactgc agactccacc
     4021 aaaacttcta aggatacctc taagctcctc taacaggggc gagatagttt tgatattcga
     4081 tgctgcaaac aactttttgc gagctttctt ttcaatctgc ctaacccttt ctctggagag
     4141 accgtagtcc tttgcaatac tctcgagggt ttcagggtac ttagcccaaa tcgccagtct
     4201 cctgtttaag atatctcttt gccggtctga aagtacggtt tcgaggaggt agatgcagtc
     4261 gtccaaagac ttcacttccc gcattgcgct tcccctgcca ttgaaccttt ccagttcatg
     4321 caaaatagct ttaagcgatc tttctccgat atttttgatg ctgaggaaat cggagtcttt
     4381 gaggttgatg atctcctcaa cgaaggaaaa gccgctgttt ttcagagcgt tgtaagccct
     4441 gctggggaga tccaacttct caagaggggt tttgctttca caggtgtgat ccaacttgta
     4501 cttctctctg ctgcttgcaa tggttttgcc ggtcaccagg gtgtaacggg tgagcagatt
     4561 gtttaaggaa gttgtcccaa taccgctaag gcttgcaagc tcttctctgc tcatgttggc
     4621 tatctggctc acgtaaaaaa tgccgttttt gctaagagca ttgagagttc tgttgtccag
     4681 tcctaggtta gcgatagcga tgtctccctc ttctctctgc ttcaagaagt gttggagagc
     4741 tctgtcgatg agttcgcagg tcttttcgcc gatacccttg aaggtgagta gggttttttt
     4801 gtcgagagct atcagctcac ctacggtgga aatgccggca tttgtcaaac ttctaaaagc
     4861 cctgcggtct aacgccagaa ccaaaaggtc aagatccatg atcgctgcta gagaaccttt
     4921 agtcggtttc ccagtgtcgc gcatcgttcg gtgaaccttt ctggttatct cagcagccgt
     4981 aacttgtcca attcttggga tcttgagtaa ttcctcatag gtcttgctgg agactttctc
     5041 cagcgtgtcc agcccaacac tcattagagc atttactgct ctagtcgaca gtcccagttc
     5101 agaaatggag atttccgaat ttagtggatc acggggtgaa gttttgacaa gatccattcc
     5161 gactaccacc ttacgttggt ttagcaaatg agcggaactt tcgtttaacc cttaatttct
     5221 ctaattgcaa gattattcct ttacgatttc cacaccaaga ttagggatat gtcaaaatcg
     5281 taggatcttt ttggcttcac ttcttaagtt ctcaaaattg catcatcctg aagggtgcag
     5341 ttatggagta tggagtgaag tcacatataa aagcttaacg ctagaccctc tccactgccg
     5401 cgataccttt tgtccaggtt tcgtgcgatg attgctaaaa gaaccagttg gtactttctg
     5461 cctaatgtgg gtaaccacag acgcttagac acttgatggg cacttagttt aaacttgaca
     5521 aaggcgacca aactacttag aattaaacca tggtcgatag tagagaaagt ttgtttctgc
     5581 aatactattc acatagacga atttgcttga ggaggttttt tagtgaaaga gaaagtagtg
     5641 gccatcttgg atagaattcg tccaagcctt caggcagacg gcggcgacgt tgaacttgta
     5701 gatgtaagcg atgacggtat cgtcaaagtc agactggtag gtgcttgtgc tggttgcccg
     5761 atgtctaccc ttactctgaa gaatggtatc gaacgcattc tgaaggaaaa cgtgcctgaa
     5821 gtacaatctg ttttggctgt ttaagacata aaaagggagt gttgcaaaac tactaattaa
     5881 gtagggagca acgctccctt ttttgt
//
LOCUS       DAOSDA010000045         5872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_8618_length_5872_cov_3.651711, whole genome shotgun sequence.
ACCESSION   DAOSDA010000045 DAOSDA010000000
VERSION     DAOSDA010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8618_length_5872_cov_3.651711"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLW82_01845"
     CDS             <1..298
                     /locus_tag="PLW82_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46047.1"
                     /translation="PGAFYMPTNRSAIMQMHMDKDILTLFCDALEEGSRSIYGLVAPR
                     NRRRYLQMAVQKCIFNGEDPEKAIVEAAKEHNAEIVKKQQEYDRFIKKLLDAKR"
     gene            complement(514..1446)
                     /locus_tag="PLW82_01850"
     CDS             complement(514..1446)
                     /locus_tag="PLW82_01850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOJ46048.1"
                     /translation="MENSIARLGPGLDQVDASVLIPTYNRKEILRKVLSGLFKQETQY
                     SFEIVIVDDGSTDGTFESLLDLDPPVAMQVIRIEHKGPSGARNAGLKISRGELVIFVD
                     SDLYPCPEFVEAHIKAHTADNLIGHGPVIHTDDLENPTDAEKKITDIARAFFATGNAS
                     IYRRHLFEAGLFDEDFNEYGWEDLEFGLRLRKRGLKKIEVPEAKGYHYKPKLVVADLH
                     KWKQRERERGHTAVLYYRKHPVYRVRLQTMISPFWLGVDRLISLGGWTEKPATEEYLK
                     KVEKQGRHLWLRFLVRLITLHSYFEGIREAQREN"
     gene            complement(1501..2460)
                     /gene="gmd"
                     /locus_tag="PLW82_01855"
     CDS             complement(1501..2460)
                     /gene="gmd"
                     /locus_tag="PLW82_01855"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOJ46049.1"
                     /translation="MRALITGITGQDGSYLAELLLDKGYEVFGLVRRLSTKNYWRIEH
                     ILDKIELIEGDITDSGSLLLALKKADPDEVYNLAAQSFVGTSWNQPVLTAEVTGIGAL
                     RLLEVILQYKPRIKFYQASTSELFGLAKEVPQKETTPFHPRSPYGVAKAFAHYMTQNY
                     RESYGMFACSGILFNHESPRRGDEFVTRKVTKAVAKIALGKQDKLVLGNLDARRDWGY
                     APEYVEAMWRMLQADCPRDYVIATGKTYSVRDLAEKAFQVVGLDYRDYVVTDERLIRP
                     AEVPLLVGDSSLAKAELGWEPKVGFEEMIAEMVQVDLEREKAK"
     gene            complement(2508..4922)
                     /locus_tag="PLW82_01860"
     CDS             complement(2508..4922)
                     /locus_tag="PLW82_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOJ46050.1"
                     /translation="MGKTLKLILVFCVFLTSICLAEAEEPSWIWFPDAGSVKGEPRYF
                     RTVFELDEIPYSLLARVVVNHETVVYINGERMRTIYPEEILGSVELVDYVVVGKNVLA
                     VEIKPKPDEEIKPLLMRVVDIDDNVVLGTGSTWRAANKECEGWLNTDFDDSNWAKVVA
                     SPTMQKPELSSAELLAMMGQFAPEVDEKVLQNASVWLTHSAEFVFTTDAPKEEFVMPI
                     VTGTNQIAAMQLAIRSGEAIGALEVRFTDLYSEDGKSVISKDNFKYSFVETWHMTNNS
                     TQTPANLLIRAPQDMPDILSDKKVINVPKDYTQSIYLQFYVPKGTPAGIYSGSVILDN
                     LGAEREVPVEIEVLPVELSDETNLKVTIWYQVDYIVTYNKVTKYSEEFWRILEYYAKT
                     MAEHRQNMFWLPIGTVNLRYNEEGQLTGNFETFDRWAEIFFKHGFKYIELSHFGGRVG
                     DWNSDFGPYRLVAYDAGLKTTREVPVSEFAYLLQEHLREKGWLEIAYAHVADEPTGQN
                     YRSWIDLALEIKKGAPDLKLMEAIHYTYLSDYLDIAIPQLDYFDQNRTRLLKDQEDGK
                     IELWFYTCWLPQGNYPNRLLDYPLYKTRILHWANFLYDAPGYLHWGYNWWNAFEVGYA
                     PGDGWIVYPTETAMVPSVRYEAMREGIEDYEYLLLHAKKTGEIAKKLGVEIDEKYRAK
                     EIARTVMTSLTRYTDDPLELLQARERLLREIAEMDQGPLCLVVTSVEESTRLTEPTEV
                     ELTVYAEDGCSVFVGGRIANEVSPGKFTATVVVTPEKPTVVVEVVLGNQIKTIERNYS
                     NWRIIN"
     gene            complement(5183..>5872)
                     /locus_tag="PLW82_01865"
     CDS             complement(5183..>5872)
                     /locus_tag="PLW82_01865"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ46051.1"
                     /translation="HYVIKDEKNYAFLRAGQFGMYYSEQDRSGHPHSHCDQLGVVLYL
                     DGLEVLTDPGTYRYNQSDFERRIMKDESYHSTFLVDDVHQGIYTSSFSYAHTVDGNGR
                     ANGRTIIGELEIGGVKAVRKVTLNQGECEVEDEYRILDGREHQLQIFFCLSPRFTPVE
                     VDQTQVLLQHIGTRKYLALKHDLGLMPEVYPGYVSREYNQAEPNQRLAFKTTLKQDLK
                     VNFKFRFVSKD"
BASE COUNT         1364 a         1549 c         1329 g         1630 t
ORIGIN      
        1 accaggtgca ttttacatgc caacaaaccg ctcggctatt atgcagatgc atatggacaa
       61 agacatccta acgctgtttt gcgacgcttt agaagaaggt tctcgctcta tctacggttt
      121 agttgcaccg agaaaccgca ggcgttatct acagatggca gtgcaaaaat gcattttcaa
      181 cggtgaagat ccggagaaag ccatagttga agcggcaaaa gagcataatg ccgaaatcgt
      241 caaaaagcag caagagtacg acagatttat caagaagctt cttgacgcca aacggtagac
      301 ttccaacact caaaggtcca tcgttttaaa agggtattat gagttcaaaa aagcgaccta
      361 cacccttcat ggttgtaggt cgtttgcttt tgacaggaga ccgatactac aattaaactt
      421 tctcaaatta acctgtcctg ttacattctt atcggtttaa cggttgactt tttccgatac
      481 atcctgtgtt catacttgat ccagacccta gcgtcagttc tccctctgag cttccctgat
      541 tccttcaaag tagctgtgaa gagtgatcaa cctcacaagg aagcgcaacc agagatgtct
      601 accctgtttt tccacctttt taaggtactc ttcagtagca ggtttttcgg tccagcctcc
      661 aagggatatt aagcggtcca ctcccaacca aaaaggcgag atcatcgtct gaaggcgcac
      721 tctatagacc ggatgcttcc ggtagtagag tactgctgtg tggcctcttt ctcgctctct
      781 ctgcttccat ttgtggaggt ccgctacaac tagtttgggt ttgtagtggt agcccttggc
      841 ctcgggaact tctattttct tcaagcccct ttttctcagt ctaaggccaa actccaggtc
      901 ttcccagccg tattcgttga agtcttcatc aaacaaaccg gcttcgaata ggtgccttct
      961 gtagatcgaa gcgtttcctg tagcgaaaaa agccctggcg atatcggtga tctttttctc
     1021 ggcgtctgta gggttttcca gatcgtctgt atggattacg ggaccgtggc cgatcaagtt
     1081 gtctgcagtg tgagctttta tgtgcgcttc cacaaactcc ggacagggat agaggtcgct
     1141 gtctacgaag attacgagct ctcctctgga gatcttaagc ccggcatttc gggcaccgct
     1201 tggacctttg tgctcgatcc taatcacctg catagccaca ggcggatcca aatccagcag
     1261 actttcaaag gtcccatcag tcgatccgtc atcaacgatg acgatctcaa agctatactg
     1321 agtctcttgt ttgaacaaac ccgacaggac tttacgcagg atttctttgc ggttgtaagt
     1381 tggaatgagc actgatgcgt ccacttgatc caggccagga ccgagccttg ctattgagtt
     1441 ttccaccaat ctgcacctct tttagtcatg tcaataaaaa agacaggaag actcctgcct
     1501 ttattttgct ttttctcgtt ccaaatccac ttggaccatt tcggcaatca tctcttcaaa
     1561 accgaccttc ggctcccaac ccagttcagc tttggccaga gagctgtctc ctacaaggag
     1621 cggtacctct gcaggtctga ttaatctctc gtcggttact acataatctc tgtaatccag
     1681 tcctactacc tgaaaagcct tctctgctaa atcccttacc gaataggttt tacctgttgc
     1741 gatcacgtaa tctctcgggc aatctgcttg gagcatccgc cacatagctt ccacatactc
     1801 cggagcgtag ccccagtcgc gccttgcatc taggttgccc aaaaccagct tgtcctgttt
     1861 gcccagggcg atctttgcca ccgctttagt aaccttgcgg gtgacaaact cgtctcccct
     1921 tctcggggat tcatggttaa acaggattcc cgagcaggcg aacataccgt acgattccct
     1981 gtaattctgg gtcatgtagt gggcaaaggc ttttgccacg ccgtacggac ttctcgggtg
     2041 gaaaggtgtg gtctctttct gaggaacctc ttttgcaaga ccgaaaagct ccgatgttga
     2101 tgcctggtaa aacttgattc taggtttgta ctggaggata acctccagca gtctcaacgc
     2161 accaatgccg gtgacttcgg ccgtgaggac cggctggttc caagatgtcc ctacaaacga
     2221 ctgggctgcc aggttgtaaa cctcatcagg atcggctttt ttcagagcta gaagcagcga
     2281 tccagagtcg gttatatctc cttctataag ctcaatcttg tctaaaatat gctcaatacg
     2341 ccagtagttc ttagtgctga gcctccgaac aaggccaaag acttcatacc ccttatccag
     2401 caggagttct gcaagatagc tgccgtcctg ccctgttata ccagtaatta aagctctcat
     2461 gttaccacct tttaagagcg gcttgccgca tgggcaagcc ggctttttta gttaattatt
     2521 ctccagttgc tgtaattcct ttcgatggtc ttaatttggt tgcccaaaac gacctccact
     2581 accactgtgg gtttctctgg agttaccaca acagttgcag taaatttgcc tggcgagacc
     2641 tcatttgcaa ttctgccacc cacaaaaaca ctgcaaccgt cttcagcata gactgtcagt
     2701 tccacttctg tgggttctgt aagccgcgta ctctcttcca ctgaagtcac aacgagacaa
     2761 agtggaccct gatccatctc ggcgatctct ctaagtagcc tttcccgcgc ttgcagcagc
     2821 tctagaggat cgtcggtata tctggtgagc gatgtcatca ccgtgcgggc gatctctttg
     2881 gcacggtact tctcgtcgat ttccacgccg agcttctttg caatctctcc agtctttttg
     2941 gcatggagca ggaggtactc gtagtcctca atgccttctc tcatggcttc ataacgcacc
     3001 gatggaacca tggcggtttc cgtgggatag acgatccaac cgtcgccagg ggcgtagccc
     3061 acctcaaacg cgttccacca gttgtaaccc caatgaaggt accctggtgc gtcgtagagg
     3121 aagtttgccc agtgaagaat cctcgtcttg tacagaggat agtccaggag cctgttgggg
     3181 tagttcccct ggggaagcca gcaggtgtaa aaccaaagct cgatcttccc atcttcctgg
     3241 tctttcagca acctggttct gttctgatcg aagtagtcaa gctgcggaat agcgatatcc
     3301 agataatcgc tgaggtaggt gtagtggatg gcctccatca gcttcaagtc cggagcacct
     3361 tttttgatct ctaaagccag atcgatccac gacctgtagt tttgcccagt cggttcgtcg
     3421 gctacgtgag cgtaagcgat ctccaaccaa cctttttctc tgaggtgctc ttgaagtaga
     3481 taagcaaact cggaaactgg aacttcccta gtggttttta ggcctgcatc ataggcaacc
     3541 agtctgtagg gaccaaagtc gctgttccaa tcgccgactc tgccgccaaa atggctcaac
     3601 tcgatatact taaagccgtg cttgaagaag atctctgccc agcgatcgaa ggtctcgaag
     3661 ttgcctgtca gctgtccttc ttcattatac cttaggttca ccgttccgat ggggagccag
     3721 aacatgttct gcctgtgctc tgccatggtt ttggcgtaat actcgagaat tctccagaac
     3781 tcctccgagt acttggtcac tttattgtaa gttacgatgt agtctacctg gtaccagatg
     3841 gtgactttca gattggtttc gtctgaaagt tccacaggaa gcacttcaat ctccaccgga
     3901 acctctcttt cggctcccaa gttgtcaagg atgacgctgc cactgtagat ccctgcaggc
     3961 gtacccttcg gaacgtaaaa ctgcaagtag atgctttggg tgtagtcttt aggcacgttg
     4021 atgacttttt tatcgctgag aatatccggc atgtcttgag gcgcccggat taggagattt
     4081 gccggcgttt gagtggagtt attggtcatg tgccaagttt ccacgaagct gtacttgaag
     4141 ttgtctttag agatgacgct ctttccgtct tcgctgtaga gatctgtaaa gcgcacctcc
     4201 aacgcaccga tagcttcacc agacctaatc gccagctgca tcgcggcaat ctggtttgtg
     4261 ccggttacaa tcggcataac aaactcctct ttaggagcat ctgtggtgaa gacgaactcc
     4321 gcgctgtggg ttaaccacac tgaagcgttc tgcagcactt tttcgtccac ctccggtgca
     4381 aactggccca tcattgccag aagttcagcg ctggaaagct ccggcttctg catggtaggt
     4441 gaagcaacta cctttgccca attggagtcg tcgaaatcgg tattcagcca accttcgcac
     4501 tccttgtttg cagcacgcca ggtgcttccc gtgcctaaaa ctacgttatc gtcgatgtcc
     4561 accacccgca taagcagagg cttaatctct tcatccggct taggcttgat ctccactgcc
     4621 aagacgttct tacctactac cacataatct accagctcga cgctgcccaa gatctcttct
     4681 gggtagatgg tccgcattct ttcaccgttt atgtagacta ctgtttcgtg attaactacg
     4741 actcttgcta atagagaata gggaatttca tccaactcga acacggttct aaagtatctg
     4801 ggctcgcctt tgacgctgcc tgcatctgga aaccaaatcc aactgggctc ttctgcttct
     4861 gcaaggcaga tggaggtcaa aaacacacaa aagaccagaa tcaacttcaa ggtttttccc
     4921 attatactcc ctcccctatg tctcactgaa aaagttcata acatgactac gatttccttc
     4981 atattctctg caggaagttt ccagccctta cccccttaac gtccaggtct acagaatctg
     5041 ccttctcttt gtctgaaact tggcgctctg cagcccttct ttgcgcgtct ttctccgacc
     5101 tttaagtcca gaaaaaagtg ctgcgctttt cccagcaagg aaagggcagc actgtttttg
     5161 tgtttagaag aataataaat tattagtcct tggaaacaaa gcgaaacttg aagttaacct
     5221 taagatcctg tttgagcgtg gttttgaatg ccaagcgctg gttgggctct gcctggttgt
     5281 actctctact tacatacccc ggatagactt ctggcataag gccgagatcg tgctttaatg
     5341 ccagatactt tctggtgccg atgtgttgga gcaagacctg tgtttggtct acctccacag
     5401 gcgtaaagcg agggcttaaa cagaagaaga tctggagttg gtgctcccta ccgtctaaga
     5461 tccggtactc atcttccact tcgcactcac cttgatttaa ggtaaccttc cgtactgcct
     5521 tcacgccgcc gatctccagc tccccgatga tggtgcgtcc atttgccctg ccgtttccgt
     5581 ctacggtgtg ggcgtagctg aagctggaag tgtaaatgcc ctgatgaaca tcatcaacta
     5641 agaatgtgct gtgataggac tcatccttca tgattctacg ctcaaagtcg ctttggttgt
     5701 agcggtaggt accgggatcg gtcagcactt ctaatccgtc taaatagagc acaactccca
     5761 gctgatcgca gtggctgtgg ggatgccctg aacggtcctg ctctgagtag tacataccga
     5821 actggccggc tctgaggaag gcgtagttct tttcatcctt gatcacataa tg
//
LOCUS       DAOSDA010000046         5866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_8628_length_5866_cov_3.880743, whole genome shotgun sequence.
ACCESSION   DAOSDA010000046 DAOSDA010000000
VERSION     DAOSDA010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8628_length_5866_cov_3.880743"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="PLW82_01870"
     CDS             <1..1532
                     /locus_tag="PLW82_01870"
                     /inference="COORDINATES: protein motif:HMM:NF025841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HOJ46052.1"
                     /translation="GSRESMRGLSAGFKTVLLVALCALIFAPEAFAFEISGINQDTSS
                     APWLFLEGANLVFTESGFRTAQVATARVDILYNFEDFDLTFEAKVEEYGLYGSIMVLL
                     HQGDNYAGYSVAIDRNRATVSRRNGFWNDYQWLKNINGALKLGTWHEVRLTCKDQVYT
                     YYLDGTKMGEFEDKDRVYTKGSIDIRIMNSLAEIRNIRVTAEKDPEPKTANIVKIDLT
                     PNRLPNPNALITGTFLQLDYTNTRDQEGSWKDELKAMKDAGLDLLIVPAYSGSTGPVY
                     SVMDELFAEANRQGISIMIGTFSNHSYYWTDLSKKQLSETLEKTKAEITDLYNRYGDN
                     PAFYGWYITDEIDEKDLIPSKFDLTTWYYSEQVNHIKSLTPEKPIMLSPSYESNTSPK
                     WWEEKYDKFLEVVKIDILGPQDSVGAKRSTPEMVSTFFQAFKNACDKHGVRLWADVEI
                     FDIDVWLPADIDLVKRQLFAVEPFVEKVVIFEFNHYMNPYKNNNTKKLYENYLQWLSS
                     F"
     gene            complement(1608..1931)
                     /locus_tag="PLW82_01875"
     CDS             complement(1608..1931)
                     /locus_tag="PLW82_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46053.1"
                     /translation="MYNQIARRGGGLSNLESTRTLECERGFPELEGNCVISLVADSGK
                     RSHRDSTIVLQIKNESQLRDFYRAITKGGKVEFELQEISSGRFQAIFSDALGVTWNLS
                     YLKPK"
     gene            complement(2176..2916)
                     /locus_tag="PLW82_01880"
     CDS             complement(2176..2916)
                     /locus_tag="PLW82_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOJ46054.1"
                     /translation="MSKIEKLVGLLQRLNSGEKIEDVQKDAEDFLNQISATDLSIAEQ
                     QLIDNGMDAEDLRGLCVIHMQMLEGEVERMTASLDEGHPLAIMIQEHDVLLKTLEELE
                     ALNATLQEKPLTPELQEELLRLAVTLTGAELHHLREEDALFPELERRGISGPPTVMRL
                     EHDDLRPKKHKLEVLAREAMEMDQEEFRHHLAALSRHIIFNLRDHIFKENHILYPAAV
                     ETITEREVWSKIEEECDKIGYTPFTKGK"
     gene            complement(3069..3797)
                     /locus_tag="PLW82_01885"
     CDS             complement(3069..3797)
                     /locus_tag="PLW82_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOJ46055.1"
                     /translation="MAERNDTIQHRSGCLQCHGVFCGERVPIFRSLEEAEFAAIAKLI
                     IHKDYDKGEIIFHEGDNFPYLAIVREGLIKTFKYTAEGREQILGMFEKGDFVGEINLF
                     SGRPCSVSCSAIQKTGLCLIPRDSFQALIKEHSQIMFHILEELAGRVDDLETLVQQLG
                     TQNTDARLTFLLVRWGERHGRRHGEDILVELPLSREGLANYIGVSRETVSRKLSQLKE
                     EGLIDLIGNRKVLIKDLDSLRERA"
     gene            complement(3944..4585)
                     /locus_tag="PLW82_01890"
     CDS             complement(3944..4585)
                     /locus_tag="PLW82_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46056.1"
                     /translation="MKDWLVLGLIFLLLVSLPAGAKWQLMVSPAQEGLREFCMIGYSW
                     DVEPFTVALHLGASMNGEAYLSEMNSYTDESLGHYMEVYGGLLIPAVNLRIEKLDYQK
                     RVVPFFQIRYARDLVRVGGGDLDIPWLPRGDAEKLELDEIESSLIYEFFRVGGGVRLK
                     VSDSFSILGEYGIDVRLVKFDQQGYEKKTLVFDERIRGVCPNTYGGVTLVWQW"
     gene            complement(4659..5672)
                     /locus_tag="PLW82_01895"
     CDS             complement(4659..5672)
                     /locus_tag="PLW82_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46057.1"
                     /translation="MLKIDNLVKNYPDFRVSVDFSVSKGEFFSLLGPSGCGKTTTLRL
                     IAGLEYPDSGSILLEGEDITKLPPQKRRFGLVFQDYALFPHLNVEKNILYGIAKENKA
                     YQQKRLQELLELFRLEYLQKRSVRNLSGGEQQRVALARALAPNPQVLLLDEPFAALDP
                     SLRESLREELKQLQERLGLTIVFVTHNQEEALSLSHRIAIMEKGIITEIARPFEVYTK
                     PKDRDSAAFFGRTNFVKLKVTGGAVQWGTISLLLEEPITDGEYLFSVRPEYIGEGKGV
                     EGVIVSSSFLGFGVLYAADTAYGVLYFLEFAPSTIKSKGSRVELGLSVKNLAPIREEE
                     QDS"
     gene            complement(5656..>5866)
                     /locus_tag="PLW82_01900"
     CDS             complement(5656..>5866)
                     /locus_tag="PLW82_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOJ46058.1"
                     /translation="MALSLGDLTGVMLVGEGRFVTFTIALYRLIGHYSFPEGTALGTL
                     VSILFIILFVFIESGGGKKSNAQDR"
BASE COUNT         1518 a         1524 c         1313 g         1511 t
ORIGIN      
        1 aaggaagcag agagagtatg cgaggtttat cagccggctt taagaccgta ctactagttg
       61 ctttgtgtgc tttgatcttt gctccagaag cctttgcctt cgagatttcg ggaatcaatc
      121 aagataccag cagcgctccc tggcttttct tggaaggagc caacttggtt tttacagaaa
      181 gtggcttccg cactgctcaa gtagccactg ccagagtgga catcctctac aacttcgaag
      241 acttcgacct cacctttgag gcaaaagttg aagagtacgg tctttacggc agcatcatgg
      301 tcctccttca ccaaggagac aactacgctg gctactctgt ggctatcgac aggaatagag
      361 caacagtatc aaggagaaac ggcttctgga acgactacca gtggttgaag aacatcaacg
      421 gagccctcaa actcggtacc tggcacgaag tgaggctcac ctgcaaggac caagtctata
      481 cctactacct tgacgggacc aaaatgggcg agttcgaaga taaagacaga gtctacacca
      541 aaggctccat cgatatccgc attatgaact ctctggctga aatcagaaac atcagggtta
      601 cggcagaaaa agaccctgag cctaaaacgg ccaacattgt taaaatagac ctaacgccca
      661 atcgcctgcc aaaccccaat gcgctgatta ccggaacctt cctccagctg gactacacca
      721 acaccagaga ccaagaaggc tcctggaaag acgagttaaa ggccatgaaa gatgcagggc
      781 tggatctttt aatcgtacct gcatactccg gctctaccgg tccggtgtac agcgtcatgg
      841 atgagctgtt tgcagaagcc aaccgccagg gcatctccat catgatcggt acgttctcca
      901 accacagcta ctactggacg gacctgtcca agaagcagct tagcgaaact ctcgaaaaga
      961 ctaaagcaga gatcaccgat ctctacaacc gctacggcga caatccagcc ttctacggct
     1021 ggtacatcac cgatgagatc gatgaaaagg atcttatccc gagcaagttc gatctcacca
     1081 cctggtacta cagcgaacaa gtaaaccaca ttaagtccct gacaccggag aaacccataa
     1141 tgctctctcc gtcatatgaa tcgaacacct ctcctaaatg gtgggaggaa aaatacgaca
     1201 agttcctgga agtggtgaag attgacatcc tcggccccca agacagcgtt ggagcaaagc
     1261 ggtctacgcc ggagatggtc tccactttct tccaggcgtt caaaaacgct tgcgacaaac
     1321 acggcgttcg tctgtgggcc gatgtggaga tctttgatat cgacgtctgg ctcccagcag
     1381 atattgatct ggtgaaaagg cagctgtttg ctgtagaacc gtttgtagag aaggtggtta
     1441 tctttgagtt caaccactac atgaacccat acaaaaacaa taatactaag aagctgtacg
     1501 aaaactatct ccaatggttg agcagcttct aagaaaacgc caaaaagagc taaggtgaaa
     1561 accctagctc tttcttctgt acaagcttga aaaacgttta cacataatca cttaggtttt
     1621 aagtagctta aattccaagt tactcctaac gcgtcagaaa aaatcgcctg gaatctccct
     1681 gaggagatct cctgtagttc gaactccact ttcccgcctt tggtaatggc tctgtagaag
     1741 tcccgcagtt gactttcgtt tttaatctgc agcactatgg tagagtctct gtgggacctt
     1801 tttccagagt ctgcaacaag agaaatgacg cagttgcctt caagttcagg aaagcctctt
     1861 tcgcactcta aagtccgtgt gctctctagg ttgcttaacc caccgcctct tctcgctatc
     1921 tggttgtaca ttctttgcct cactctcctt cgcgccgcag taaaggtaga tccattgtag
     1981 atcggatatc ccttagtata tgcaggacga aggcaaatta taccaaaccc ggcttaataa
     2041 ttttcggcct gaaagcagta tttagaagtt tgacgcaaag tccctaaatc tcggcgaatt
     2101 cttccgtcta ttctgcaggc tgttcgccaa aaaaggccct ggactccacg tggagccagg
     2161 gccgattatt tccagctact tacctttggt aaaaggggtg tagcctattt tgtcgcattc
     2221 ttcttcgatt ttggaccaga cttctctttc ggtgatggtc tctactgcag cgggataaag
     2281 gatatggttt tctttgaaga tgtgatctct aaggttgaag ataatgtggc gtgaaagagc
     2341 cgctaagtgg tgtctgaact cttcctgatc catctccata gcttcccgag ccaaaacttc
     2401 cagcttgtgc tttttcggac ggagatcatc gtgctcgagc ctcattaccg taggaggacc
     2461 tgaaatacct cttctttcca gttcagggaa aagggcgtct tcttccctta ggtggtgcag
     2521 ctctgctccg gtcaaagtta ccgcaagcct aagcagctct tcctggagtt ccggggtaag
     2581 cggcttttct tggagagttg cattcaacgc ttctagttcc tctagggtct tcagaagaac
     2641 atcgtgctct tgaatcataa tggctagtgg gtgaccctcg tcgagactag cagtcattct
     2701 ttctacttcg ccttcaagca tctgcatgtg gatcacgcac aagccgcgaa ggtcctctgc
     2761 atccattccg ttgtcaatga gctgctgctc ggcaatggag agatctgtag cgctgatctg
     2821 gttcaaaaaa tcttcagcat ctttctgcac atcttctatt ttctcaccgg agtttagcct
     2881 ttgcaaaagg ccaaccagtt tttcgatttt gctcatgcaa aacgctcctt tcaagttcaa
     2941 ggttttcgct cagaatacaa agcgtattaa cgcaaaactc ggggtttgtt gatgctttaa
     3001 atttacctaa cctgaaagga taaatctgtg acagctgtca caaaaagagc ttgaggcaga
     3061 aaatttcctc aagctctttc tcttaaacta tccaggtctt tgatgagcac cttgcggttg
     3121 ccgataagat cgattaaccc ctcttccttg agctggctta gctttctgct tacggtctcc
     3181 cgagacacgc cgatgtagtt tgccagtccc tcgcgggaaa gaggaagttc cactaaaatg
     3241 tcttctccat gacgtcttcc atggcgctct ccccaacgga ccaaaagaaa ggtgagcctg
     3301 gcgtcggtgt tttgggtccc cagctgctga accaaagtct ccaaatcgtc aacccggccg
     3361 gccagctcct caagaatgtg aaacatgatc tgagagtgct ctttgatcag tgcctgaaaa
     3421 ctatccctgg gaattaggca gagaccggtc ttttggatgg ccgaacagct cacgctgcac
     3481 ggtctaccgg aaaagaggtt gatttcgccg acgaagtctc ccttttcaaa catacccagg
     3541 atctgctccc ggccttcggc tgtatactta aaggtcttga tcaatccttc tcgcacgatc
     3601 gcaagatacg ggaagttgtc cccttcgtga aagatgatct cgcctttgtc gtagtctttg
     3661 tgaataatca gctttgcgat tgcggcaaat tccgcctctt ctaagctgcg gaaaatcggc
     3721 acccgttcgc cgcaaaaaac cccatgacac tggaggcatc cagatctatg ctgaattgtg
     3781 tcgtttcttt ctgccatgga ctcactcctc atcttcagca gcgttttgtt attgttaatt
     3841 cagtaaaaag cagaacattt gctggaggtc tttcctaatg catagcctag atctgtttaa
     3901 ttatagacgc aggcttctgt tcctctttta aggagtaact tacttaccac tgccaaacca
     3961 gagtcactcc tccatacgtg ttagggcata cccctctgat gcgctcatca aacactaggg
     4021 tcttcttctc gtagccctgt tggtcgaact tcaccaatct aacgtctatt ccgtactcgc
     4081 ccagaatact gaacgagtcc gagaccttga ggcggacacc gcctcccact cggaaaaact
     4141 catagattag gctggattct atctcatcca gctcaagctt ttccgcgtct cctctaggta
     4201 gccagggaat atcgagatct ccgcctccta ctctaaccaa atccctagcg tacctgatct
     4261 ggaaaaacgg cacaactctc ttttggtagt ccagcttttc aatgcgtagg ttaaccgctg
     4321 ggatgaggag accaccgtaa acctccatgt agtggcctaa agattcgtcg gtgtagctgt
     4381 tcatctcaga caggtaggcc tcgccgttca tacttgcgcc aagatgcagg gcaacagtaa
     4441 acggttccac gtcccaactg tacccgatca tgcagaattc tcgaagtcct tcctgagctg
     4501 gagacaccat cagctgccac ttagcgcctg ctggtagact caccagcagc aaaaaaataa
     4561 gccccaatac caaccagtct ttcacaaaaa ctcctcctct ggtttaatat ggtgcttatt
     4621 ctaggtgttg ttcttcgaat atattttgcc gatctacctc aagaatcctg ctcctcttct
     4681 ctgatcggag caaggttctt caccgaaaga cccagttcaa ctctggatcc tttagatttg
     4741 atggtagacg gtgcaaactc cagaaagtac agaacaccgt aagccgtgtc tgcagcatac
     4801 aggaccccaa agcccaaaaa gctgctggaa acgatcactc cttcgacccc tttaccctcc
     4861 ccgatgtact ccggtcggac ggaaaagaga tactcgccat ctgtgattgg ttcttctaga
     4921 agaagactaa tggttcccca ctggactgca ccacctgtaa ccttaagctt gacaaagttc
     4981 gttctgccaa aaaaggcagc cgagtccctg tctttaggct tagtgtagac ctcaaagggc
     5041 ctagcgatct cggtaatgat tcctttttcc atgatggcaa tacggtggga aaggctcaaa
     5101 gcttcttctt ggttgtgggt tacgaaaact atagtcaagc ccaatctctc ctgaagctgc
     5161 ttcaattctt ccctcaaaga ctcccgcaac gacggatcta aagcagcgaa aggttcgtcg
     5221 agaagaagga cttggggatt gggagcaaga gctctagcga gagctactcg ctgctgctcg
     5281 ccacctgaga gattccggac cgaccttttc tggaggtatt ccagacgaaa gagctccaat
     5341 agctcctgca atctcttttg ctggtacgct ttgttctctt tagcgattcc gtaaagaatg
     5401 ttcttttcta catttagatg ggggaagaga gcgtagtcct gaaagaccaa accaaacctg
     5461 cgtttttgtg gcgggagttt ggtgatgtcc tcaccttcaa gcagaatgct tccgctgtca
     5521 ggatactcca gtccggcaat gagcctcaag gtcgtggttt tgccacagcc actcgggccg
     5581 agaagggaaa agaactcgcc ttttgacacg gagaagtcta cagaaactcg aaagtccggg
     5641 tagtttttta ccaaattatc gatcttgagc attgcttttc ttccctcctc ctgactcgat
     5701 aaacacaaac aagatgataa agagaatgga cactagcgta cctaaagcag tgccttcggg
     5761 gaagctatag tggccgatga gtctgtaaag agcgatggtg aaggtgacga accgaccttc
     5821 tcctaccagc atcacaccgg ttagatcgcc cagagaaagg gccata
//
LOCUS       DAOSDA010000047         5854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_8656_length_5854_cov_3.429729, whole genome shotgun sequence.
ACCESSION   DAOSDA010000047 DAOSDA010000000
VERSION     DAOSDA010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8656_length_5854_cov_3.429729"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLW82_01905"
     CDS             complement(<1..183)
                     /locus_tag="PLW82_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46059.1"
                     /translation="MRKFVFILLCLLMTASAFALDLGSVELEQSSGTSQGGTKTHFSP
                     GSVGGAITPSKTTLAAL"
     gene            complement(732..1259)
                     /locus_tag="PLW82_01910"
     CDS             complement(732..1259)
                     /locus_tag="PLW82_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46060.1"
                     /translation="MKKNLFVRAVFLAFFALFLGGCVVIVKPIEYHPEKLITKDLAKA
                     VIWENYSDIMRCFSSDWVKVVSGRQIRYWDQEDAADFFYFEPNPQLRGTTRFDFTLLR
                     VDFDVYPDVARVYGELYMQGLRPYQGPGSIATFKFKGKIEFTVKRLYNDKWVITGLDL
                     SNFELDEGPIQTHSL"
     gene            1373..2197
                     /locus_tag="PLW82_01915"
     CDS             1373..2197
                     /locus_tag="PLW82_01915"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOJ46061.1"
                     /translation="MRLGLHVSISGKLTRAFDEGRNSRSEALQIFTASPRMWKSAQIS
                     EEAAAEFRAEKNDFSPIVVHSSYLINLGSKSPEIREKSLQALLEEVERCKMLGADFLV
                     LHPGGGDENAVTYIAEGLSKALDSAGGVKILLENVAGEGSKVGKNFHELKAIIDETTN
                     GERLGVCFDTCHAFAAGYDLRRKDVWLKLHEEIDATVGFAAVKLVHLNDTKGELGSNL
                     DRHANWTEGKLGVETLKIIRDDPRFSSVPGILETPGDLAARTADLKLCRELLNFEG"
     gene            2187..4124
                     /locus_tag="PLW82_01920"
     CDS             2187..4124
                     /locus_tag="PLW82_01920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOJ46062.1"
                     /translation="MKGKVEEFFLNILPEKMPGYEARHVQLEMAIGIAEAFTSAHKAI
                     IEAGTGTGKSLAYLVPSFFTDKDSRVAISTGTKNLQEQLFKKDLPFLARIWQEVYGEE
                     ISFMYVQGRSNYICLTRLRNTLIPEDDRALLSKWIKETEDGNINELTGRLHPETVRNI
                     VSDPDYCLSQKCPFRTDCFITRLRVKAQRTKILVVNHHLLLTDLMIKDSVGADQNSIL
                     PALSHIVLDEAHHLEEAATECFGEEVSFDALHGLRNQVINNRELQRQDIELKTRIEQE
                     LWDAVPHINRAKELGETMDREDTAWGMQTDAALVAGNIRQHYREAAQLLAEYGDTSVE
                     AEALSRKVNRVVRSLDAFLDDKNDRVSWLDRQGFHSVLLSVREALRRILFSAVEPVAL
                     TSATLSTSGNFRYIKSRLGLDSGFTPRELILPSLFDYSKVLLAIPTDLLNPNSEKDIE
                     RIADHLREIVPRIKGGSFILCTSFKMVRIVKEKLQDLEGINLYVHGDKNAQELIASFR
                     ADGRGVLIGVDSFWEGVDVPGDALKAVFITKLPFPVPSEPLFAARQRLVEERGGNSFR
                     ELSLPAALLKLRQGFGRLIRRQSDEGLVVIYDSRALYKSYSRDVFQSLPKCREWRGKL
                     DEMAAFIENDNYLRTTLFQDS"
     gene            4147..4971
                     /locus_tag="PLW82_01925"
     CDS             4147..4971
                     /locus_tag="PLW82_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HOJ46063.1"
                     /translation="MLFALGFPSLAVEHVVGPGENLTQIAKLYNTEVAQILSVNGITD
                     ADKIWVGQKLIIPNNKLVIKLDEVGTKESQERPQNTYQSYVHLSYSILPSGYNGQEHV
                     CVVGAEDTVDEIAKRYGVSADLILTRNGLSAEQELRPGQILFVPTDTFKLIYSTDKEV
                     ELLARVISAEARGENLEGQIAVGAVILNRVKDPRFPNSITEVIFQKGQFEVVSNGTYL
                     TVPVTEQARIAAREALRGRDPTNGALFFYNPRIAKNGWYFETRVKAVEIGNHVFTY"
     gene            5021..5719
                     /locus_tag="PLW82_01930"
     CDS             5021..5719
                     /locus_tag="PLW82_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ46064.1"
                     /translation="MFIIIRRSQLLFILIAAVLVVLLLLFVIIPRAVLSSSGSLPIFK
                     GDPNKKAVAFCINVAWGNEYIPSILDTLKTHDVKATFFFVGTWVQENPDLGRTIAEAG
                     HEIANHGYSHVHPKKLSKSSIQKLITDNEAVLLKEFGRASKVFSPPYGEFNSKIVDAA
                     AEIGYKTVMWTLDTIDWQNPDAATFLGRIVPKLENGALILMHPTEVTAKNLGTLIAEI
                     KSRGYDIITVSEIL"
BASE COUNT         1564 a         1443 c         1474 g         1373 t
ORIGIN      
        1 taatgctgca agggtggtct tcgaaggggt gattgctccg cccacagatc caggagaaaa
       61 gtgggttttt gtaccgcctt ggctagttcc acttgactgc tccaactcaa cggacccaag
      121 gtccagcgca aacgcactag ctgtcatcag aaggcataat aatataaaaa cgaatttacg
      181 cattgctaaa cctccctgac ctatcccagc ctgtccaagc tgggcacaac ctcgcccgga
      241 ccagcagaaa acgaatcgct atagtccagg taatcccggg cgttttccgt ttcagctgaa
      301 gagagtccga gatctaacca ggtcttgcct tttttcagag gcttgtcttt tggctgtcct
      361 gtccaaagaa gcagttcctc tggaagctac gtttttgcag cttgtccgaa gacccaaccg
      421 gcgaggcaac tacaaagctg atagtctgcc aaacaactgc ttccaaaggc ttagcgcagc
      481 taagcagcgt cttggaagtt ctcccagggg aacttgtcta atctcacttt gcagatagag
      541 tatgcggcat ctggaagatt atgcacattc tagaaagatt tttttaggta gtaatgaatt
      601 accggtaaat taaacgggga agccttatgg caagcggaaa acctccgctc gttcttgcac
      661 tccgaaagct ccctgggcaa aaaaaaagag ccctttaggg ctctgtatat cggtagaggc
      721 agagatatgc cttacagaga atgagtttgt attggtcctt catcgagttc gaaattggat
      781 agatcaagac ctgtgatcac ccacttgtcg ttgtacaggc gttttacggt gaattcgatt
      841 tttcctttga acttaaacgt tgcgatgctg cccggaccct ggtagggtct tagcccctgc
      901 atatacagct cgccgtacac tctggcaacg tcgggataga cgtcgaagtc caccctcagg
      961 agggtgaagt cgaacctagt ggtgcccctt aattggggat tgggttcgaa gtagaagaag
     1021 tctgcagcgt cctcttgatc ccaatacctg atctgacggc cgcttacaac cttaacccaa
     1081 tctgatgaga agcagcgcat aatgtcactg tagttttccc atattaccgc tttggccagg
     1141 tctttcgtaa tgagcttttc cggatgatac tcgatcggtt tgacgatcac cacacagccg
     1201 cctaaaaaca gcgcaaagaa cgcgagaaaa accgcccgta caaaaaggtt ctttttcata
     1261 cagaccaact ccaatccgat aaccatattt ttgcaggagt accaacgcga gaagccgaaa
     1321 agggtcgaga gtaccatttt tatgaaagga gaggccactt ggcctgaaag ctatgagatt
     1381 aggactgcac gtttcaatat cgggaaaact caccagagct tttgacgaag gcaggaattc
     1441 taggtctgaa gctctgcaga tatttacagc aagcccaaga atgtggaaaa gcgctcaaat
     1501 ctccgaagaa gcggcagcgg agtttagagc cgagaagaac gatttcagcc ccattgtagt
     1561 tcactcttcc tacctgatca acctgggttc caaaagcccc gagatccggg agaagtctct
     1621 gcaagccctg ttggaggagg tagagcgctg caagatgctt ggagcagact ttctggttct
     1681 tcacccaggt ggaggcgacg agaacgcggt aacttacatt gccgaaggac tgtctaaagc
     1741 tttagactct gcaggcggcg taaagatact tttagaaaat gtagcaggtg aaggcagtaa
     1801 agtcggcaag aacttccacg agctgaaggc tattatcgat gaaaccacaa acggagagcg
     1861 ccttggcgtc tgttttgaca cctgtcacgc ttttgctgca ggatacgatc tccgcaggaa
     1921 ggacgtgtgg ctcaagctgc acgaagagat agacgctaca gttggctttg cagctgttaa
     1981 gctcgttcac ttaaatgata ccaaaggcga gctgggtagc aacttggaca ggcatgccaa
     2041 ttggacagaa ggtaaactgg gagtagagac gctgaagatt atcagggacg atccaaggtt
     2101 cagttctgtt cccgggatcc tcgagactcc aggagacctt gccgcaagaa cagcagacct
     2161 caaactctgc agggagttgt tgaactttga agggtaaagt cgaagagttt tttctgaaca
     2221 tactgcccga aaagatgccc ggctacgagg cgcgccacgt ccagctggaa atggccatcg
     2281 gcatcgccga ggcttttacc agcgctcata aagcgatcat tgaagcaggg accggcacgg
     2341 ggaagtccct tgcctatctt gtgcccagtt tctttactga taaagactcc cgcgttgcca
     2401 tttccaccgg caccaagaat ctgcaggagc agctgtttaa gaaagacctg ccctttcttg
     2461 cccgcatctg gcaggaggtc tacggtgaag agatttcctt catgtatgta caaggccgca
     2521 gtaattatat atgcctgacc aggttgcgaa atactttgat tcccgaagat gacagagcac
     2581 tgctttctaa gtggatcaag gaaacagaag atggcaacat caacgaactg acaggaaggc
     2641 tccaccctga gacagttagg aacatagtct ccgacccaga ctactgcctg agccagaagt
     2701 gtccttttag aaccgactgc ttcatcacaa ggctgagagt caaagcccaa agaaccaaga
     2761 tcttggtggt aaatcaccac ctgctgctca cagacctgat gattaaggat tctgtaggtg
     2821 ccgatcagaa ctcgatcctg cccgctcttt cgcacatcgt tttagacgaa gcccatcatc
     2881 ttgaggaagc cgctacggaa tgctttgggg aagaagtatc ctttgatgct ctccatggtc
     2941 tgagaaatca ggtgatcaac aaccgtgagc tgcaaaggca ggatatcgaa ctgaaaaccc
     3001 gaatcgaaca ggaactctgg gatgcagttc ctcacatcaa cagggctaaa gagctcggag
     3061 agacgatgga cagggaagac acagcatggg gcatgcaaac cgatgctgcc ctggttgctg
     3121 gaaatatcag acagcactac agagaagcgg ctcagctcct tgcagaatac ggcgatacct
     3181 ctgttgaagc ggaagctctc agcagaaagg taaacagagt tgtaagaagc ctcgatgcct
     3241 tcctagatga caagaatgac agagtcagct ggctggacag gcagggattc cacagcgttc
     3301 tcctcagcgt acgtgaggcg ctccgcagaa ttttgttttc agcggtggag cctgtagcgc
     3361 tgacttctgc aactctttcc accagcggaa actttaggta catcaagtcg cgtcttggtt
     3421 tggatagtgg ctttacacca cgagagctga ttttgccgtc ccttttcgac tactcgaaag
     3481 tccttcttgc cataccgaca gatctcctca atcccaacag cgagaaggat attgagagaa
     3541 ttgcagatca cctcagagag atagtaccga ggatcaaggg aggaagcttt atcctctgca
     3601 cctcgtttaa aatggttagg attgtcaaag agaaacttca ggacctcgag ggcatcaacc
     3661 tgtacgtcca cggcgacaaa aatgcccagg agctgattgc ctctttccga gccgacgggc
     3721 ggggagtgct catcggcgtc gacagcttct gggagggtgt ggacgtgcca ggagatgcgc
     3781 taaaggctgt cttcattacc aaactgccgt ttcccgttcc ctccgaaccg cttttcgccg
     3841 caaggcagag gctcgtggaa gagcggggcg gtaactcctt tcgtgaactc tcccttccgg
     3901 cagctctcct aaagctgcgg caggggtttg gcaggctcat ccgccgccag agcgacgaag
     3961 ggttggtagt gatctacgac tcgagagccc tttacaaaag ctacagccgc gatgtgtttc
     4021 agtcattgcc aaaatgcaga gaatggaggg gaaagctaga tgagatggcg gcattcattg
     4081 agaacgacaa ctacctgcgc acaacgctct ttcaggacag ctagagtctg gttgttctgt
     4141 gcagctttgc tctttgcttt aggttttccg agcttagccg ttgaacacgt cgttggcccg
     4201 ggagaaaact taacacagat cgcaaagctg tacaacaccg aagtcgctca gattctgagc
     4261 gtaaacggta tcaccgacgc agacaagatt tgggtaggcc aaaagctcat catccccaac
     4321 aacaagctgg tgatcaaact cgatgaagtg ggaacgaaag agtcgcagga aaggccgcag
     4381 aacacctatc agtcctacgt tcatctttcc tacagcattc ttccttctgg ctacaacggc
     4441 caagaacacg tctgcgttgt aggagcggaa gacactgttg atgagatagc caaacgctac
     4501 ggtgtttccg cagatctcat tcttacccgt aacgggctca gcgccgaaca ggagctgaga
     4561 ccgggacaga ttctttttgt tccaacagat accttcaagt tgatctactc cacagacaag
     4621 gaagtggaac ttttagcgcg cgttatttct gctgaagcta gaggagaaaa cctcgaaggt
     4681 cagattgcag tgggcgctgt aattctcaac agggtcaaag accccagatt tcccaactcc
     4741 attaccgagg tgatctttca gaagggccag tttgaggttg tctccaacgg cacctatctc
     4801 actgttccgg tgacagagca ggcccgcatt gcagccagag aagcactgcg cgggcgggat
     4861 cctacaaacg gagcgctctt tttctacaac cccagaatag ccaagaacgg atggtacttt
     4921 gaaaccaggg ttaaagccgt tgaaatcggc aaccacgtct ttacttatta agcataaggc
     4981 gagctcagca ggcatactct ctcttaggag ggatatgcct gtgtttatta ttataagacg
     5041 ttcccagctt ctgtttattc tgatagctgc ggtcctggtt gttctccttt tgctttttgt
     5101 cattatacca agagccgttc tgtcgtcttc tgggtctctt ccgatcttta agggagatcc
     5161 caacaaaaag gcagtggcct tctgcatcaa tgttgcctgg ggcaacgaat acatcccgtc
     5221 tatcttagac acgttaaaaa cgcacgatgt gaaggccaca tttttctttg taggcacgtg
     5281 ggttcaggag aaccccgatc tggggagaac cattgctgaa gccgggcatg agatcgccaa
     5341 tcacggctac tcccatgtcc acccgaaaaa gctttctaaa agcagcattc agaagctgat
     5401 caccgacaac gaagctgtgc tcctcaaaga gttcggcaga gcctcgaagg ttttttctcc
     5461 cccttacgga gagttcaata gcaaaattgt cgatgcagca gcggaaatag ggtataaaac
     5521 agttatgtgg actctggaca ccatagattg gcagaatccc gatgcagcga cttttcttgg
     5581 ccgcatcgtc ccgaagttgg aaaacggtgc gctgattctc atgcatccta cggaggtcac
     5641 tgcgaaaaat ctaggaacac tcattgctga aattaagtcc agaggatatg atattattac
     5701 cgtaagtgaa atcctgtaat ataatggaag ttcaccaaca actcgggagg tggagacaag
     5761 tctctgactt gtagctgatg attgaaaaaa gcgtattgcc aaatggccta actatcttaa
     5821 cagaatccct accgcacctg cgctctgtgt ctgt
//
LOCUS       DAOSDA010000048         5749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_8869_length_5749_cov_2.540745, whole genome shotgun sequence.
ACCESSION   DAOSDA010000048 DAOSDA010000000
VERSION     DAOSDA010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8869_length_5749_cov_2.540745"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLW82_01935"
     CDS             complement(<1..519)
                     /locus_tag="PLW82_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46065.1"
                     /translation="MKTMQSKEKNQREYSLRKQERQKEHSVKKSIFIGFCIGIIMPLI
                     PVFIYFVVLILPRLVTYLYFRVSGGDYQTELLIVQRFTRNWGSLLTVIAAITGLFGAW
                     GAVNGYLRKLSFRDPRALKYHSKGIVPCTVFAAGLFIFLVLNAKLNVFGITFLSVLFG
                     LVGGIAFMGYYTF"
     gene            611..1318
                     /locus_tag="PLW82_01940"
     CDS             611..1318
                     /locus_tag="PLW82_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HOJ46066.1"
                     /translation="MPQGLIVLIRSTFSFFTLFVLTRVMGRRQISQLNFFDYVNGITI
                     GSIAATLAIDISRESLPTFIGLLAYTFWVLFLNLLEEKNRTLRRIINGKSVIVIQNGK
                     ILENNLREYGYTIDDLKMILRTQGAFNLADVEYAILEANGRISTLLKSQVRPATPKDF
                     GIDTEYQGIPIDLISDGKIIADNLNLVGLTEDWLRGKLSELRLKPEDVFYAELDTSGK
                     LYVDAYRDPHPPKEVKG"
     gene            1321..1665
                     /locus_tag="PLW82_01945"
     CDS             1321..1665
                     /locus_tag="PLW82_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46067.1"
                     /translation="MNRTTLGLIVIFVVIVGLVLLIEYVPDKNFSQRFHTLSLAIDSE
                     AYEIAHMEYAKIRDTWGRKKFLYGLENSVERIEEIDLTLERLGFYLSSRDRTNALLET
                     KLLAFHWRQLAE"
     gene            1747..2505
                     /locus_tag="PLW82_01950"
     CDS             1747..2505
                     /locus_tag="PLW82_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOJ46068.1"
                     /translation="MEYTIKKLASLAGITTRTLRYWDSIGLLKPKRITSSGYRIYGKE
                     EVDRLQQILFFRELAFSLDEIKELLANEDFDHLNALREHKLRLLEERNRLNTLIDTVE
                     KTISEKEGRLTMTDKEKFAGFKREMIDENEKKYGKEARKLYGDEAVEKSNQKLLNMSE
                     EQYKELTQLTEEILTKLVEAKHQGDPSSPLAQEVAALHKKWLTFFWPSYSKEAYRSLA
                     QTYVDDPRFTAYYDAKESGCAEFLKDAIFIYTRD"
     gene            2616..3044
                     /locus_tag="PLW82_01955"
     CDS             2616..3044
                     /locus_tag="PLW82_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ46069.1"
                     /translation="MGDLRLFGQFPDLFDAFKSELLPRIGSFTYPPIDVTETADELVI
                     RAEVPGIDPKNLDVEIGESAIVLRGKADEAREVTSQGYRRLERRSGSFERMISYPIPV
                     LHQEAKASLRNGLLEIKIPKDKTKYRGMRKLAIEFDQNQN"
     gene            complement(3095..3916)
                     /locus_tag="PLW82_01960"
     CDS             complement(3095..3916)
                     /locus_tag="PLW82_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46070.1"
                     /translation="MKVAKCYPEKGMDCKSIVQKAKNLGLDGLAVSAISGSCAHFKTE
                     KMRHSGFALRELRDAVQAEGLQFGLIFPFFHDEHVWNKSPERRASLPEKDLAWYRPLC
                     PVYPGLFEERLSLFLEATCEIKPDFVGLDFFRFPLFWEGIGESMEVKTCSCESCSADG
                     RRSEVIREYAEVIMEKIEGLRACVHLVPFTIGEVQEATGQDPQALAGAGLVLSPMLYH
                     KLLKREESFVGEALKLLESFQVWPSVELGSAFFEDTSGTLQGYEKVIYFYFDLKK"
     gene            complement(3918..4478)
                     /locus_tag="PLW82_01965"
     CDS             complement(3918..4478)
                     /locus_tag="PLW82_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ46071.1"
                     /translation="MRSIIVGITGGSGSGKTTLAKKLVEYLGPDIVTRVPLDNYFFPN
                     ASTNNAPEAVDFSKVHAQLDALLRGEEVVTTRGNLLTPNEIYIVEGHLVMCDPDLVQR
                     LDLSIYVDLDPEERLLRRIQRNVGFGMELQGIIDWFRKDVKHNFKRHVEPQKELCDLI
                     VWGEITERRVEILAELILRIKEIGRE"
     gene            complement(4481..>5749)
                     /locus_tag="PLW82_01970"
     CDS             complement(4481..>5749)
                     /locus_tag="PLW82_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46072.1"
                     /translation="GLFDRDPQGTPFAGSGTKIRGENYGKEGMMWRSQSIEEFGQREI
                     ERVRKKHPERLLLHGLEMNLPEGGHAGVFINSSDGSALARFEYIFDTCDRDLSLNLPK
                     HNRDGEGAIRAVRYLAERHKDQSFFIVNHPHVYKAFTPRLIRSLNDAAPEVVVGFEGI
                     PGHQYRRARGYYLYEKGEDFRFYRLYGGADYMLAKVGGLWDSLLGEGRRFFVFSGSDF
                     HAAGDDLDPYPGEYSKTYLYLEHLTPEDLIASVRGGRGFVVLGDIIQGLDFTLQSEEI
                     AGLGDELQTHDGKVLLEIKVAVKDPLTLHHIDVIEGEVGERFLPESPGYDSAENGSAQ
                     VVLRIERRDLCEIEPGVFQYRARLNLRFNKGYLRLRGTNLPPNTDFETDPDGNPLIDD
                     HSTRRNTKEIAQNDCWFYTNPIFYKEKKRC"
BASE COUNT         1488 a         1549 c         1337 g         1375 t
ORIGIN      
        1 aaaggtgtag taacccatga aagcaatccc accaactaaa ccgaaaagta cgctcaaaaa
       61 ggtaataccg aacacattga gtttagcgtt caaaacgagg aaaataaaca gacctgcagc
      121 gaacacagtg caaggcacga tgccctttga atggtactta agtgcccttg ggtccctaaa
      181 gctgagcttt cgcagataac catttaccgc accccatgca ccaaaaagcc cggtgatagc
      241 agcaattacc gtaaggagac taccccagtt cctggtgaac ctttggacta tcagaagctc
      301 tgtttgatag tcgcctcctg aaacccgaaa gtacagatag gtgaccagcc tcggcaaaat
      361 cagcacgaca aagtagataa aaaccggaat cagaggcatt ataatgccaa tacaaaagcc
      421 aatgaagatg ctctttttca ccgagtgctc cttttgtctt tcctgctttc ttaaagaata
      481 ctccctctgg tttttctctt tcgattgcat ggttttcacc ttccttcctg ccatcggcga
      541 agtactagcc acagcaaact tctattattc cttccgaatc aggtcatcat aagatgtggg
      601 aggtagttat atgccgcagg gactaatcgt gctgatccga tcgacttttt ccttttttac
      661 cttgttcgtc ctaaccagag tcatgggccg aaggcagatc tcacagctaa acttcttcga
      721 ctatgtaaat ggaatcacca tcggttctat tgctgcaaca cttgccatag acatttcccg
      781 cgagtcactc cccacattca tcggtcttct cgcctacacc ttttgggtcc tgtttctcaa
      841 cctgctcgag gagaaaaacc gaaccctacg ccgaattatc aacggcaaga gcgtcatcgt
      901 cattcaaaac ggcaaaatcc tggagaacaa cctcagagag tacggttaca ctatcgatga
      961 cttgaagatg atcctcagga ctcagggagc cttcaacctt gccgatgtgg agtacgccat
     1021 tttggaggca aacggcagga tttccacact tttaaagagt caggtgaggc cggcaacgcc
     1081 taaggacttc gggatagaca ccgagtacca gggaatccct atagacttaa tctccgatgg
     1141 gaaaatcatt gcagacaacc ttaacctagt aggattgact gaggattggc tcagggggaa
     1201 gctttctgag ttgaggttaa aacctgaaga cgtgttctac gctgaactgg acacctcagg
     1261 aaaactctac gttgacgctt accgcgatcc ccaccctccc aaggaggtta aaggctgaag
     1321 atgaacagaa cgacactagg actcatcgtg atttttgtgg tgattgtcgg actggtcctt
     1381 ctcatcgagt acgtaccgga taaaaacttc tcccaaagat tccacaccct gtctctagct
     1441 atcgacagcg aagcctatga aattgcccac atggagtatg ccaaaatccg cgacacctgg
     1501 ggcagaaaga agttcctcta cggactggaa aacagcgtcg aacgcatcga agagatcgac
     1561 ctcactttag agcgccttgg cttttacctg agcagccgcg accgaacgaa tgcgctgctc
     1621 gagaccaaac tcctcgcctt ccactggcgt cagcttgcag aataactaag gtttttgcct
     1681 tagttatttt tttcttgcct ttgacgtagc gtaaaggttt atagttgagg tgaaaggagg
     1741 ttttgcatgg aatacaccat caagaaactt gcaagtcttg ccggaatcac caccaggaca
     1801 ctgcgctact gggacagcat tggactctta aaacccaaac gcatcacctc atcgggctac
     1861 cgtatctacg gcaaggaaga agtagatagg ctccagcaga tcctcttctt cagagagctg
     1921 gccttcagcc tcgacgaaat caaggaactc cttgcaaacg aagacttcga tcacctcaac
     1981 gcgttgcggg agcataaatt acgtctgtta gaagagcgaa acaggctaaa cacgttgatt
     2041 gatacggtag aaaagaccat ttcggaaaaa gaagggagac taactatgac tgataaagaa
     2101 aagtttgccg gcttcaagcg cgaaatgatt gacgaaaacg aaaagaagta cggtaaagaa
     2161 gcccgaaaac tttacggaga tgaagctgtg gagaagtcca atcaaaagct cctgaacatg
     2221 agcgaagagc agtataagga actcacccag ctcactgaag aaatcctcac aaaactcgtg
     2281 gaagccaaac accagggcga tccgtctagc cccctcgccc aagaagtagc tgcactgcac
     2341 aaaaagtggc ttaccttctt ctggcccagc tactctaaag aagcctacag aagcctggca
     2401 cagacctacg tcgacgatcc gagatttacc gcgtactacg atgccaaaga atccggctgc
     2461 gcagagttcc tgaaggacgc aatcttcatc tacaccaggg actagtctgc gaaaaccccg
     2521 ggactcctct acagatattt actccggttt cataacagaa gtatcaccag ggcataccta
     2581 tattagagaa aaccacttta ccaggaggag tatccgtggg tgatttgagg ctttttggac
     2641 agttcccaga tctctttgat gcctttaaaa gcgaactcct gccgcgaatt ggctcgttca
     2701 cctaccctcc tatcgacgtt accgaaaccg cagatgagct ggtgatcaga gctgaagttc
     2761 ccgggatcga ccccaagaat ctcgatgtgg agatcggcga aagcgcaatt gtgctcaggg
     2821 ggaaggccga tgaagccagg gaagtcacct ctcagggcta cagaaggctg gaaaggcgct
     2881 caggcagctt cgagaggatg atctcctacc ccatcccggt tctccatcaa gaagccaaag
     2941 cctcacttag aaacggtctc ttggagatca aaatccccaa ggacaaaacc aagtatcgcg
     3001 gcatgaggaa acttgccatc gagttcgacc aaaaccaaaa ctaaaagctg ccttctaggt
     3061 gtctgcctag aaggcttttt aaaagcaaag tcccctactt cttcagatcg aagtagaagt
     3121 agatcacctt ttcgtagccc tggagggttc cagaggtatc ctcgaagaac gccgaaccca
     3181 gctctacaga aggccaaacc tgaaagctct ctaggagctt caatgcctca cctacgaagc
     3241 tttcctccct ctttaaaagc ttgtggtaca gcataggcga aagtacaagg cccgcaccgg
     3301 ctagagcttg gggatcctga ccggtggcct cttgcacctc gcctatagta aagggcacca
     3361 gatgaacgca agcacggagc ccttcgatct tttccatgat gacttcggcg tattccctga
     3421 tcacctcaga tctgcgcccg tcagcgctgc agctttcgca gctgcaggtt ttaacctcca
     3481 tgctctcgcc tatcccctcc caaaacagcg gaaagcggaa gaagtccaga cctacaaaat
     3541 ccggcttgat ctcacaggtt gcttcgagaa agagggaaag cctctcttca aaaagcccgg
     3601 ggtacaccgg gcatagaggg cggtaccagg cgagatcctt ctcaggaaga gatgctctcc
     3661 tttcggggct cttgttccag acgtgttcat cgtggaagaa aggaaagatg agaccgaact
     3721 ggagcccttc ggcctgtacc gcgtctctca gctcccgcag agcaaatccg gagtggcgca
     3781 ttttttcagt tttgaagtga gcacagctcc ccgagatcgc tgaaaccgcc agcccgtcca
     3841 gaccaaggtt ttttgccttc tggactatgg atttgcagtc catgcctttt tcgggatagc
     3901 actttgcaac cttcacttta ctccctacct atctctttaa ttctgagaat cagctctgcc
     3961 agtatctcca ctcgtctttc agtgatttct ccccagacga tgaggtcaca gagttctttc
     4021 tgaggctcta cgtggcgttt gaaattgtgt ttaacgtctt ttctgaacca atcgatgatc
     4081 ccctgaagct ccattccgaa accgacgttg cgctggattc tccggagtag cctttcttca
     4141 ggatctaaat ccacatagat gctgaggtca agcctctgca ccagatccgg atcgcacatc
     4201 acaaggtgac cttcaactat atagatctcg ttcggcgtaa ggaggttccc tcttgttgtc
     4261 acaacctcct ctcccctaag gagcgcgtcc aactgggcgt ggaccttcga aaaatccacc
     4321 gcttccggag cgttgtttgt ggacgcattg gggaagaagt aattgtcaag tgggactctg
     4381 gtcacaatgt caggtcccaa atattcaact aacttctttg ccagggtggt cttcccggac
     4441 ccgcttccac ctgtaatgcc aacgatgata gaacgcattt tcagcacctc ttcttctctt
     4501 tgtagaatat cgggttggtg taaaaccagc agtcattttg ggcgatctcc ttggtgtttc
     4561 tgcgcgtgct gtgatcgtca attaaggggt taccatcagg gtctgtctca aaatcggtgt
     4621 ttggaggaag gttggtgccc ctgagcctca ggtagccctt gttgaagcgg agattaagcc
     4681 gcgctctgta ctgaaagact ccaggctcga tctcacaaag atcccgcctt tctatcctta
     4741 agaccacctg ggcagatccg ttctcggcac tatcgtaacc tggactttcc ggcaagaacc
     4801 tttctcctac ctcgccctca atgacgtcga tgtggtgaag tgtcaagggg tcttttaccg
     4861 ctactttgat ttcaagaagc acttttccgt cgtgcgtttg cagctcatct cctaggccgg
     4921 caatctcttc gctctgcagg gtaaagtcca ggccttggat aatatctcca agaacaacaa
     4981 agccccggcc gccccttacg ctagcgataa ggtcttctgg tgtcaggtgt tctaggtaca
     5041 ggtaggtctt gctgtactcc ccaggatagg gatccagatc gtcacctgca gcgtggaagt
     5101 cggagccgga aaagacaaaa aacctcctcc cctctcccag gaggctgtcc caaaggcctc
     5161 cgacttttgc cagcatgtag tctgctccgc cgtagaggcg gtagaacctg aagtcctcac
     5221 ctttttcgta gaggtagtaa ccccttgctc tgcggtactg gtgccctgga atcccttcaa
     5281 accccactac aacctctggc gctgcatcgt taaggctgcg aatcagccta ggcgtaaaag
     5341 ctttatagac gtggggatgg ttgacgatga aaaagctctg gtctttgtgg cgttcggcca
     5401 gataccgcac ggctcggatg gcgccttcac cgtctctgtt atgcttgggc aagttcaaac
     5461 tgagatccct atcgcaggta tcgaagatgt actcgaacct ggcaagagcg cttccatcac
     5521 tgctgttgat gaaaactcct gcatgtccgc cttcagggag gttcatctcc aaaccgtgaa
     5581 ggaggagtct ttctggatgc tttttcctca cccgttcgat ctctctctgc ccaaactcct
     5641 ctatggactg agatctccac atcatgcctt ccttgccgta gttctccccg cggatcttcg
     5701 tcccgctgcc ggcaaaggga gtgccctgag ggtctctatc gaacagacc
//
LOCUS       DAOSDA010000049         5661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9025_length_5661_cov_3.161613, whole genome shotgun sequence.
ACCESSION   DAOSDA010000049 DAOSDA010000000
VERSION     DAOSDA010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9025_length_5661_cov_3.161613"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /locus_tag="PLW82_01975"
     CDS             complement(<1..1736)
                     /locus_tag="PLW82_01975"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ46073.1"
                     /translation="MLKLLNGLDKRTVEFIRLKLQDSNLEIISIADNFTRGIVEFFGL
                     RRSVRDLLLGVDPWTGHIWPSKHYKELFFDPGHSPGDVKFLWEFMKHNFLPALGKAYR
                     LTGDASYAKSGVEFIKEWLKSVEDERGASWAGHPHICQRITNWLFFFELIEGSEAVGI
                     SEKQEIDSYLNKQWRLLEKEYERPANNHRLISICFVIMGRLYNNSASDLEFWLGELKS
                     TTETLFLPDGGFSEQSTSYHRFSLESLLLASQGLKNAGYEIPSWLQDAVERSLVYFDA
                     VLEPSGQPPVFGDNSDEIFIVREWPEKFWDMEYLFAFGRSLGFNVRKPWITPPSLFWI
                     IGLGKVEPGGKPEQPLQAAFPDSGHFVLRDGEGSYVFFRAGSFGLHIPGETWWAHSHS
                     DQLGLVAYLEGIEILTDPGTYRYNENDFERMIMKDESFHSTVMIKGVKQAKYSSSFIY
                     KDALDAKGTLEDRVISGVLETEDFRIQREVQLEDRKLVITDRILARPGSEAEWYFALS
                     PNLSPVELEPGKVVLQEIGSLKKVTLSISEEVSLEVLPGYVSYLYNRALPSQRLHFGL
                     KVVGVKVVKFVFA"
     gene            complement(1822..2334)
                     /locus_tag="PLW82_01980"
     CDS             complement(1822..2334)
                     /locus_tag="PLW82_01980"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOJ46074.1"
                     /translation="MESIRHEFLQLAKDLGAVDAVIFELDDIVFDPRTLVKCMYGCAD
                     WGKRHTCPSRPGSLKPWEYEQIFKRYSWGLIVHTHDLKLSQEISYKLESEAFVRGYYF
                     AFSLSDCALCKECTAGFGKDCVHPKKARPAFHSVGIDVFKTVRKFGLPLETLKDETEQ
                     QNWYSAVFIE"
     gene            2514..3803
                     /locus_tag="PLW82_01985"
     CDS             2514..3803
                     /locus_tag="PLW82_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOJ46075.1"
                     /translation="MLKKRLLLSLTIVMLIACFAQAKTLEIWYVGWTNEFKAVAQNII
                     DADFVAKTGVDVNIQVLSWSDYYNRYLLALASRDTPDIFVLGTETVDFGIRGGLMDLA
                     KFKPEEFKEKEAQIFGSLMGPFSFKGTRFGVPVSIGAITAAYRADILTDLGMSVPKTW
                     DDIKQWQPKALAQGHVFGLYYGSVQYDSMWGAYTLITQNGGQFFNPDGFSSALDKPES
                     IKGFQEYISLFKDHNFPLAGAGLTPFTTGEWLMLTDGLWLYPNLLQHAPELRGKWVMD
                     LVPGTIQEDGSIHHGSYVSSSVMGISPYAKNPEEAWEFIKWFSSEEIQTKVSNAILQQ
                     VPGSMWLPANKNALQKVEIGEDAKAAIFGQLTHSEPVPYAINVNVLYRYVTFAIDKSV
                     LQNVDPKEAVLEAAKEMNEEMARRQREYSRLLATLGN"
     gene            complement(3897..4766)
                     /locus_tag="PLW82_01990"
     CDS             complement(3897..4766)
                     /locus_tag="PLW82_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOJ46076.1"
                     /translation="MSKSKAQLSLVFISIVWGLSFLVLSDGVEFLAPETLLFWRFLTS
                     AAVLFLIKKVTKNKKKLPWKEGLIAGVLFAVGYLSQTQGILYTSPSVAAFLTGLLVVF
                     TPIFEGIVRRKAPRINVWISSFLALFGTYLLVNGGAEAELIGVILQITCAVFFALHVV
                     YIGSKPNVDPIGFVTVQSLFVAAVCLVLAGFNGTLMLPGQGISAVLYLGILASALGLT
                     VQVKAQKVIPPSEASIIFALEPVFATLFSALILSERITLPSFIGMILIFSATIIVDLN
                     GKLRKRGEVKKAT"
     gene            complement(4763..5500)
                     /locus_tag="PLW82_01995"
     CDS             complement(4763..5500)
                     /locus_tag="PLW82_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOJ46077.1"
                     /translation="MTSNVAIETVDLKKNFGSLEVLKGISLTVKKKEVVCVIGPSGSG
                     KSTFLRCLNLLEKPTSGSVFIDGVDITSKDCDIDSVRANMGMVFQNFNLFPHRRVLDN
                     IILAPMKVKKLQRDEAEEIAINLLAKVGLKDKAKSYPDELSGGQKQRVAIARALAMQP
                     EVMLFDEPTSALDPEMIGEVLEVMKALAFDGMTMIVVTHEMGFAKEVSDRVLFMDEGL
                     VLEDDTPQNIFNNPQNPRTQAFLSKVL"
BASE COUNT         1485 a         1440 c         1285 g         1451 t
ORIGIN      
        1 gcaaaaacaa actttacaac ctttacgcca actaccttaa gcccaaagtg cagtctctga
       61 ctgggaagag ctcgattgta caggtaggag acgtagcctg gaagaacctc tagagagact
      121 tcttccgata tgcttagcgt caccttcttt agagaaccaa tctcctgtag aaccactttc
      181 cccggttcca gttccacggg gctgagattt ggactgaggg caaagtacca ctccgcttcg
      241 cttccaggtc ttgccagaat cctgtcggta atcaccagct tacgatcttc aagctgaacc
      301 tcccgctgga tacgaaaatc ttccgtctcg agaactcccg agatgacccg gtcttctagc
      361 gttcccttgg catcaagagc gtccttataa ataaatgagc tgctgtattt agcttgtttt
      421 actcctttga tcatcactgt ggaatgaaag ctttcgtctt tcatgatcat ccgctcgaaa
      481 tcgttttcgt tgtagcggta agttccgggg tctgtgagga tttcgatccc ctctaggtaa
      541 gcaacaagac cgagctggtc cgagtggctg tgagcccacc aggtttcgcc gggaatgtgc
      601 aggccaaagc tgcccgctct aaagaagaca tacgaccctt cgccgtctcg tagaacaaaa
      661 tgcccagaat cgggaaaggc tgcttggagg ggctgttctg gtttgccacc gggttcaact
      721 tttcccagtc cgataatcca aaacagtgac ggtggagtga tccaaggctt tctaacatta
      781 aagcccagcg atcttccaaa ggcaaagagg tactccatat cccagaactt ctcgggccac
      841 tccctaacga taaagatctc atcggagttg tcgccgaaaa caggaggctg tccgctaggc
      901 tcaaggactg catcgaaata cacaagactg cgttctaccg catcctgcag ccagctgggg
      961 atctcataac ctgcgttttt taacccttgg gacgcaagaa gcagactctc taacgagaat
     1021 cgatggtagc tggtgctctg ctcggaaaag cctccatcgg gcaaaaagag agtttcggtg
     1081 gtactcttca gctcaccgag ccaaaactcc aaatcggaag ctgagttgtt gtacagcctg
     1141 cccatgatca caaagcatat ggagatcaac ctgtggttgt ttgccggcct ttcgtactct
     1201 ttttccagaa gccgccactg tttgttgaga tagctgtcga tctcctgctt ttcactaatg
     1261 cccactgcct ctgaaccctc aataagttca aagaaaaaca gccagttagt gatacgctgg
     1321 cagatgtgag ggtgtcctgc ccaactggct cccctttcat cctcaacgct cttcaaccat
     1381 tctttaatga actctacccc agactttgcg tagctcgcat cgccggtaag cctgtaagct
     1441 ttgccaagag caggaaggaa gttgtgtttc atgaactccc acaggaactt tacgtcccca
     1501 ggagagtgtc cagggtcgaa aaacagctcc ttgtaatgct tgctaggcca gatgtgaccg
     1561 gtccaggggt ctactcccag caggaggtcg cgaacacttc ttctcagtcc gaaaaactcc
     1621 acgatgccgc gcgtaaagtt gtcggcaatg gagatgatct ccaagtttga gtcttggagt
     1681 ttcaatcgga taaactctac cgtccttttg tccaatccat ttaacagctt aagcaaatca
     1741 ctacctccga acttattcta agttgtggtt tgtaaaaaat cttctactca tcttgaacaa
     1801 gactttgtgc ccttgggagt tttactcgat aaacaccgca gagtaccaat tctgctgctc
     1861 agtttcatcc ttcaaagtct caagaggcag gccaaacttg cgcaccgtct tgaacacgtc
     1921 gatgcccacg ctgtgaaaag ccggcctggc ttttttgggg tgcacgcagt ccttgccaaa
     1981 tcctgcagtg cactctttgc acagggcgca gtcgctgaga gaaaaagcga aatagtagcc
     2041 ccggacaaag gcctcgcttt cgagtttgta cgatatttcc tggctgagtt tgagatcgtg
     2101 agtgtgaacg atgagccccc aactgtacct cttgaaaatc tgctcgtact cccaaggttt
     2161 taatgatccg ggcctcgacg gacaggtatg ccttttcccc cagtctgcgc aaccgtacat
     2221 acatttcacc aaggtcctcg ggtcaaagac aatgtcatcc agttcaaaga tcactgcgtc
     2281 taccgcacct aagtcttttg ccagttgtaa aaattcgtgt ctgatgcttt ccattgctag
     2341 ggcctccatc aaatttaata ctcacgtatt tctgtctttt cccggaaagt cctccagaca
     2401 aacatttgat gggatgagtc gtccgtttcc cgaaggaaaa aagaggttag taaagaagta
     2461 cttgtatggt tttaatcatc tttcatcatc acatctttag gaggcgttat ttaatgctca
     2521 aaaaaagact ccttttgagt ctaacaatcg ttatgttgat tgcctgtttt gcccaggcaa
     2581 aaactttaga gatctggtat gttggctgga ctaatgaatt taaagctgtg gctcaaaaca
     2641 tcattgatgc cgattttgtg gcaaaaaccg gcgtcgatgt caatattcaa gttttgagct
     2701 ggtctgatta ctacaacagg tacctgcttg ctctagcaag ccgtgatact ccagacatct
     2761 tcgttttagg tactgagact gtggactttg gtattcgcgg cgggttgatg gaccttgcca
     2821 agttcaagcc cgaagagttc aaggaaaagg aagcccaaat cttcggttct ctcatgggtc
     2881 cgttctcctt taagggcaca agattcgggg ttcccgtttc catcggtgcc atcactgcag
     2941 cctaccgcgc agatatccta actgacttag gcatgagcgt tccaaagacc tgggatgaca
     3001 tcaaacagtg gcagccaaag gcactcgctc aaggacacgt cttcggtctc tactacggtt
     3061 ctgttcagta cgactccatg tggggcgcct acacccttat cacccaaaat ggcggtcagt
     3121 tcttcaatcc ggacggtttc tccagtgctc tggataaacc cgagtccatc aaaggtttcc
     3181 aagagtatat cagcttgttc aaagaccaca acttcccgct tgcaggtgct ggtctcactc
     3241 cattcaccac aggcgagtgg ctgatgttga cagacggtct gtggttgtac cccaaccttc
     3301 tacaacacgc tccagaactt agaggtaaat gggtcatgga tctagttccc ggcacgatcc
     3361 aggaagacgg ctccatccac cacggttcgt atgtcagctc cagcgtaatg ggcatttccc
     3421 catacgccaa aaaccctgag gaagcttggg agttcattaa gtggttctct tctgaagaaa
     3481 ttcaaaccaa ggtgtccaat gccattttgc aacaggttcc aggaagcatg tggctgccag
     3541 caaataagaa cgctctgcaa aaagtagaga tcggtgaaga cgccaaagct gcaatcttcg
     3601 gtcagctcac ccattcagag cccgtaccgt atgccatcaa cgtcaacgtt ctgtaccgct
     3661 atgtaacctt tgctatcgac aagtccgttc tccagaacgt agatcctaaa gaagcggttt
     3721 tggaagcagc aaaagaaatg aacgaagaaa tggcaagaag acagcgtgag tactccaggt
     3781 tgctggcaac cttaggcaac taaaagcaag atatgtaata aacccgatca agcaaaaaag
     3841 ctggcgaaag ccagcttttt ttacatacta agaagcattt gtctaatgaa atatgtctat
     3901 gtcgcttttt tcacttcacc gcgtttgcgc aacttgccgt tgagatcaac gatgatggta
     3961 gcactgaaga tcagaatcat tccgataaac gacggcagcg tgattctttc gctgaggatc
     4021 aatgcagaaa aaagcgttgc aaagaccggc tccaaagcaa aaatgatgct ggcctcgctt
     4081 ggcggaatga ccttctgtgc ttttacctgg acggtgagtc ccaatgctga tgccaggatt
     4141 cccaaataca acaccgcgct tatgccctgg cctggtagca tcagagtccc gttgaagcct
     4201 gccaaaacca aacagaccgc tgctacaaaa agcgattgta cggtaacaaa accgattgga
     4261 tccacgttgg gcttcgagcc gatataaact acgtggagag caaaaaacac cgcacaggtg
     4321 atctgcaaga taacgccgat gagctctgct tccgctccgc cattaaccaa aagataggtc
     4381 ccaaagagag ccaggaagct gctgatccag acattgatcc taggagcttt gcggcgaacg
     4441 atgccctcga atatcggtgt aaaaacaact aatagaccag ttaaaaatgc tgctacagat
     4501 ggactggtgt agagtatgcc ctgggtctga ctgagatagc cgacggcaaa aagaacccca
     4561 gcgataaggc cttccttcca ggggagtttc tttttgtttt tggtgacctt cttgatgaga
     4621 aatagtactg ctgcggaggt aaggaaccgc caaaaaagca gcgtttctgg tgccaaaaac
     4681 tctaccccgt cgctgaggac cagaaaactt aatccccaaa caatggaaat gaaaaccagc
     4741 gaaagctgag ctttagattt actcatagca ctttcgatag aaacgcctga gttctgggat
     4801 tttggggatt gttgaagatg ttctgcggcg tgtcgtcttc aagaaccagt ccttcgtcca
     4861 taaaaagcac cctatcggaa acctctttgg caaaacccat ttcatgtgtt actacgatca
     4921 tggtcatgcc atcaaaggcc agggctttca taacttcgag aacctctccg atcatctcgg
     4981 ggtccaaagc cgaggtaggc tcgtcaaaga gcattacttc cggctgcatt gccagtgccc
     5041 tagcaattgc gaccctctgt ttttgtcctc ccgacagctc atcgggatag ctcttggctt
     5101 tgtctttcaa gcctactttc gctaagagat tgatggcgat ctcctctgcc tcatctctct
     5161 gaagcttttt caccttcatc ggtgccaaga tgatgttatc cagtactctt ctgtggggga
     5221 acaagttaaa gttttgaaaa accataccca tgttggcgcg aacactgtcg atatcgcaat
     5281 cttttgaagt aatgtcaact ccgtcgataa aaacagaacc ggaagttggt ttctcgagga
     5341 gattcagaca cctgagaaag gtgcttttcc cggaaccaga tggcccaatg acgcagacca
     5401 cttccttttt cttgacggta agtgagatcc cttttaagac ctccagactg ccaaagttct
     5461 ttttcagatc caccgtttcg atagctacgt tacttgtcat tggttccgag cctcctctct
     5521 aagaagctta ccagcttcga aatactaaac gtcataacta agtaaatgat cgatgcggca
     5581 atgagaatat ccaacggcct gtagtctctg gtgtagatga gttgtgcttt acgcatcagt
     5641 tcttctaagg caatgacgct a
//
LOCUS       DAOSDA010000050         5568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9200_length_5568_cov_3.339017, whole genome shotgun sequence.
ACCESSION   DAOSDA010000050 DAOSDA010000000
VERSION     DAOSDA010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9200_length_5568_cov_3.339017"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PLW82_02000"
     CDS             complement(<1..669)
                     /locus_tag="PLW82_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46078.1"
                     /translation="MKRAFLTCILLISILSTASLAKTLEIWITGWTNEFASIAEDLVA
                     TEYTPKTGVDVNITPVGWQDGNKITLAIISNDAPDIVTAGVELGVRGSLIDLRETFGQ
                     EFEELEARLFPAITQQTHFAGTRFAIPQNISLMNAVYRTDLLAEMGMEIPVTWDDVKQ
                     MRPKLNANGMEMAFHYGSPDYGGLWGAYTLITQHGGNFYKTDGFTSAMDETESIRGFT
                     EYIEL"
     gene            complement(751..2031)
                     /locus_tag="PLW82_02005"
     CDS             complement(751..2031)
                     /locus_tag="PLW82_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46079.1"
                     /translation="MRKRGLVLLLVLSLLSVAAFAKTLEVWIVGMTNEQARIAEDIIA
                     TDYMPITGVEVNITPIAWADQNKITLAIISNDAPDIVTGGVELGVRGSLIDLRKTFGD
                     EYEELEARLFPAITQQTHFAGTRFAIPQSISLMNAVYRVDLLAEMGMEIPVTWDDVRQ
                     MRPKLNANGMDMVFHYGSPDYSGLWGAYTLITQHGGNFYKTDGFTSAMDEPESIRGFT
                     EYIELYTVHGMPQSVTGITQFRSGELVMMIDGYWAYTNLLNSAPEIAGKWEAALIPGT
                     LRADGTINHGTFTGGTHLSIPVTTKDKQAAWDFIKWFLSDDVQRKFVDSIITRIPGSM
                     MIPSSMDALYNLQGLPGNIARVIHAQINESVPVPYVPTASVLHRYINFAIQACLQQGA
                     DPREEAIKAAQEMNKEMDRRRLEYKRFLDQLGSS"
     gene            2718..4934
                     /locus_tag="PLW82_02010"
     CDS             2718..4934
                     /locus_tag="PLW82_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ46080.1"
                     /translation="MKKGLSSTKLTTKIALMNAVIVLLAVALGAFFSVRSLTSNITDL
                     LHKDALSTASFVNAEIETILLPKTALVETLATTLAVQSFQYDHVIDPALTLMESNSDI
                     TRFYLVNAMDSTSLILSRTGDRVYVDTSADAFGAKEQDWLLDTIFEQRTIFTTPQKVL
                     GSDSLAFTISTPVRNQSGQLAGVLAVDVSLADFHDIMQSITVGSNGYVMVLDSSGFVL
                     YHPDESKLLDDATAYMGTRGEVAANMVQRKSGKGEIEIDGVEYLVYYSPINLTGWSVA
                     TCLPKLELNRPMRQAITQTVITSAIILVGSAAIMVPLVRHLVRPLSMMVRQLREIASG
                     RGDLTRRLEVKNNDEVGMLASTFNDFLDTLANIIGQVQQSAQEVLRGTKELALATEQQ
                     AKTNEQIAMVIGQVAEGSQNQSQDIHRGQEAMHQLVSAIEQIAAGARSQAVQIDNVVN
                     LTQSMIEMLNGTVKAVDEITVKEKDNVKQAKRGLEIVNLVVENMAEVQNEIDETLQNS
                     TLLEQGSQQIGEIVRVINDVADQTNLLALNAAIEAARAGEHGRGFAVVADEVRTLSDR
                     VRNSSNEIGAIIQQLIKTIGDTVVNITKSTELINKGSELANKAQASLSSIVSSATESG
                     IALDGVANTNAELMTKSKEVEEAMMSIIAISQENSSAAEQMSADSSEVFRLIEAVAAV
                     SEENAASSEEVAASAEEQSASSEEVFAQADALRSIAENLGSIVANFKTNSVLEQEE"
     gene            complement(5070..>5568)
                     /locus_tag="PLW82_02015"
     CDS             complement(5070..>5568)
                     /locus_tag="PLW82_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46081.1"
                     /translation="AGKWQAGLIPGTRRPDGTINHGTFTGGTHFAIPTTTKDKQAAWD
                     FIKWFLSAETQRKFTDAIIIGIPGSLMVPSSMEALYTLEGLPREIAHVIHEQIDQSAA
                     VPYAPTAGVLYRFVNFAIQSCIQTGADPAEEAKKAAEEMNREMDRRKVEYKRFLDQLA
                     KEYSS"
BASE COUNT         1437 a         1317 c         1404 g         1410 t
ORIGIN      
        1 aagctcgatg tactcggtaa aacccctgat ggactcggtc tcatccatag ctgacgtgaa
       61 accatcggtt ttgtagaagt tgcctccgtg ctgggtgatc aaagtgtagg ctccccacag
      121 tcctccgtaa tcagggctgc cgtaatgaaa cgccatttcc ataccattgg catttagttt
      181 aggtctcatc tgtttaacgt cgtcccaggt caccggaatc tccatgccca tctctgcaag
      241 caggtcggtt cggtagacag cattcattag actgatgttc tgcgggattg cgaatctcgt
      301 accggcgaaa tgggtttgtt gggtgatcgc tgggaagagt ctggcttcca actcttcgaa
      361 ctcctggcca aaagtctctc taaggtcgat tagagaccct ctcacgccca gttctacacc
      421 ggcagtaacg atatctggtg cgtcattgga aataatggcc aaggtgattt tatttccgtc
      481 ctgccacccg acgggagtga tgttaacgtc cactccggtt ttgggagtgt actcagtagc
      541 aaccaggtct tctgcaatac tggcaaactc attggtccaa cctgtaatcc agatctccag
      601 agtttttgcg agagacgctg ttgacagaat ggatataagt aaaatgcagg ttaaaaacgc
      661 ccttttcata tacaccctcc tacagtgttt gttgccaaaa gaagacgaac actacaaaaa
      721 gagaccgcga agcggtctct cctgtcaagt tcaactgctt cccagttgat ctaagaagcg
      781 cttgtattca agtctccttc gatccatttc tttgttcatt tcctgagcgg ctttgatggc
      841 ttcttctcta ggatcggctc cttgctggag acaggcttga atggcaaagt taatatatct
      901 gtgaagcacg cttgcagtag gaacgtaggg aactggaact gactcgttaa tctgagcgtg
      961 gatcactctt gcgatattgc caggcaggcc ttgaagattg tagagcgcgt ccatagaact
     1021 tgggatcatc atggaacctg gaattctggt aatgatggag tccacaaact tcctctgaac
     1081 gtcatctgac aggaaccact tgatgaagtc ccaagctgct tgcttatcct tcgtggtcac
     1141 gggaatagag agatgagtgc ctccggtaaa cgtaccgtgg ttgatggtgc cgtcggctct
     1201 cagtgtgccg ggaatcaaag cagcttccca cttaccagcg atctctggag cactgttcaa
     1261 aaggtttgtg taagcccagt agccgtcgat catcatcacc agttcgccgg acctaaattg
     1321 ggtgatgccg gtaacagact gaggcatgcc gtgcaccgtg tagagctcga tgtattcggt
     1381 gaagcctctg atagactccg gctcatccat ggccgatgtg aagccgtcgg ttttgtagaa
     1441 attgcctcca tgctgagtga tcagcgtata ggcaccccaa agaccggagt agtctgggct
     1501 gccgtagtgg aagaccatgt ccataccatt ggcgttcagc ttaggccgca tctgtctgac
     1561 gtcgtcccaa gtcaccggaa tctccatgcc catttcagca aggaggtcta cgcggtatac
     1621 ggcattcata aggctgatgc tttgcgggat ggcaaatctc gtcccagcaa aatgcgtctg
     1681 ttgagtaatc gctgggaaga gtctagcttc cagctcttca tactcatcgc cgaaggtttt
     1741 tcttaggtcg atcaaagaac cccttactcc aagttctaca ccaccggtaa cgatgtccgg
     1801 tgcgtcattg gagatgatag ccaaggtgat cttgttttga tcagcccagg caatgggagt
     1861 gatgttcacc tccacccctg ttatcggcat ataatctgtg gcaatgatgt cttctgcaat
     1921 acgtgcctgt tcattggtca tgcctacgat ccagacttct aacgtcttgg caaaggctgc
     1981 gaccgatagt agagatagaa ccaaaagcaa aactaaaccc ctttttctca caaaacccct
     2041 cctaaaatat gtaattactt ataaaaggtt ggatgttttt gccaattttc ctgtcacact
     2101 gaaacaattt cgatttttga ttgatcatta acgtagaaga gagagcgggt gctgatgcat
     2161 cgacgcgcca aagcaaaaca acgttagatt ctgcgcatct gcacagattt ttggacatat
     2221 gctcctctat ccgtcattta tgaatagtac agggagttgg agccttttga acatgttgcg
     2281 gaaaatgtgc gataattact ctggaggttt tcatatcttc tcatccgctt tatccaaacc
     2341 accttcatga acattcaacg cttcaatgtg tttgcactgg cattaacaag cgccatatac
     2401 ttgaggttga ggtatatgtg cttttgcccg caagacatgg cgaagcataa gcgtgatcgg
     2461 agaccggggg aagaagatcg aagtcgcggt cttttttttg ttgaccctga ctaccttatt
     2521 tttccagggt cttcacctga gtgttgatca tgtacctaga tgagcgttct aaaggctggt
     2581 tcaccgactt gcctcgtttt gcaaggaaca gggtatctga ggttttcctg tctgctgcac
     2641 ggataaaagc accggggggg gcggtaccga tattcgaaaa ccttcataca accaaataaa
     2701 ccaggaaggt gaagcgaatg aagaaaggcc tttccagcac gaaactcacc acgaaaatcg
     2761 ctttgatgaa cgcagttatc gttcttttag ctgtggcttt aggcgccttt ttcagcgtgc
     2821 gaagcctcac aagcaatatc acggatctcc tacacaaaga tgctttgagt acagcttcct
     2881 ttgtaaatgc tgagattgaa accattctgt tgcctaaaac cgcactggta gagacccttg
     2941 ctaccacttt ggcagtacag agttttcagt acgatcacgt tatcgatcca gcacttaccc
     3001 tcatggaatc aaactccgac attaccaggt tttacttggt caatgctatg gattcgacat
     3061 ccctgattct ttcgagaacg ggggataggg tttatgttga cactagtgcc gatgcttttg
     3121 gtgctaaaga acaggattgg ttgctggata ccattttcga acaaaggacc atcttcacaa
     3181 caccgcaaaa ggtgttgggg tctgattcac ttgcttttac catctctacg ccggtaagaa
     3241 accagtcggg acagttggct ggggtgcttg ccgtagacgt atcgcttgct gatttccacg
     3301 acataatgca gagcataact gtaggcagca acggctacgt tatggtgctc gacagctcag
     3361 gttttgtgct ctatcatcca gatgagagca agctgctgga cgatgcaact gcttacatgg
     3421 gtacacgcgg agaagttgct gcgaacatgg ttcaaaggaa gtctggcaaa ggcgagattg
     3481 agattgacgg cgtggagtat ctggtctact acagtccgat caatctgaca ggatggtctg
     3541 tggcaacttg cttgcctaag cttgaactaa accgtccgat gcgccaagcg attacgcaga
     3601 ccgtcataac atccgccatc atccttgtgg gttctgcggc aatcatggtt ccgcttgtaa
     3661 ggcatttggt gcgccccctc tctatgatgg tacgacaact aagagagatc gcctcaggaa
     3721 ggggagacct cacaagaagg cttgaagtga agaacaacga cgaagtgggc atgctcgctt
     3781 cgacgtttaa cgactttttg gatacccttg ccaacatcat tggccaggta cagcaatctg
     3841 ctcaggaagt tctccggggg acaaaggaac tggctctagc cactgaacag caggcaaaga
     3901 ccaacgaaca gatcgccatg gtcatcggtc aggttgcaga aggttctcaa aaccaaagcc
     3961 aggatatcca ccgtggtcag gaagccatgc atcagctggt ttccgctatc gagcagatag
     4021 ctgctggagc tagatcgcag gcggttcaaa tcgacaatgt ggtgaacctg actcaatcta
     4081 tgatcgagat gctaaacggc actgtaaaag ctgtagatga aatcaccgtt aaggaaaaag
     4141 ataacgtcaa acaggcaaaa agaggactgg aaatcgttaa cttggttgtg gagaacatgg
     4201 ccgaggtgca aaacgaaatc gacgaaactc tgcaaaactc cactcttctc gagcagggtt
     4261 cccaacagat cggtgagatt gttagagtta tcaacgatgt agctgaccaa accaacctcc
     4321 tggctctaaa tgctgccatc gaggctgcaa gggccggcga gcacggtagg ggatttgccg
     4381 ttgttgcaga tgaggtaaga actctctcag atcgagtgag aaactcgtcc aatgaaatcg
     4441 gtgccatcat tcagcagctt attaaaacca tcggcgatac tgtggtgaac atcaccaaga
     4501 gtacagagct catcaacaaa ggcagcgagc ttgctaataa agctcaagcc tcgctcagca
     4561 gcatagtcag ttcagccacc gaatcgggca tcgccttgga cggtgtggca aacactaacg
     4621 ccgaactcat gacaaagagt aaggaagtgg aagaggcgat gatgagcatc attgccattt
     4681 cccaggaaaa cagttctgct gcagagcaga tgagtgcaga cagctcggaa gtcttcagac
     4741 ttatcgaagc ggttgctgca gttagcgaag aaaacgctgc aagcagcgag gaagtcgcag
     4801 cttcggcaga agagcagagc gcaagctctg aagaggtgtt cgcacaagcc gatgctttga
     4861 ggtctattgc cgagaactta ggaagcatcg tagcgaactt caagaccaac tcagtcctag
     4921 aacaagagga gtaaactgca gaaaagtctt tggtgtagaa acccacgtga aaaaagaaaa
     4981 aagccccctt tttacaggga gcgtgtgtat agaaacaagc tgcaggaacg ccaagtggcg
     5041 acctgcagct tgtttcgttg gattgctttt taactagagt attcttttgc cagctgatcc
     5101 aggaaacgct tgtactcgac ctttctgcgg tccatttctc ggttcatttc ctctgcagct
     5161 tttttagctt cttctgcagg atctgcaccg gtttggatgc aggactggat ggcaaagttc
     5221 acaaagcggt agagaacacc agccgttggt gcataaggca ctgcggcgga ctggtcgatc
     5281 tgttcatgga tgacgtgggc aatctctctg ggcagacctt ccaaggtgta cagagcttcc
     5341 atcgagcttg gaaccatcaa ggaacctggt atgccaatga tgatggcatc ggtgaacttt
     5401 cgctgggttt ctgcagacag gaaccatttg atgaaatccc aagcagcttg tttgtctttg
     5461 gtggtagtcg gtatggcaaa gtgcgttccg ccggtaaagg taccgtggtt gatggtgccg
     5521 tccggtcttc tggttccggg gattagaccg gcttgccact taccggca
//
LOCUS       DAOSDA010000051         5463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9423_length_5463_cov_3.279216, whole genome shotgun sequence.
ACCESSION   DAOSDA010000051 DAOSDA010000000
VERSION     DAOSDA010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9423_length_5463_cov_3.279216"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /gene="rsfS"
                     /locus_tag="PLW82_02020"
     CDS             <1..353
                     /gene="rsfS"
                     /locus_tag="PLW82_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOJ46082.1"
                     /translation="GGVLVEAKKIVDLVVRAAEEHKGIDLEILDVSQLTIVADYFVVV
                     SGRSTLHVDALTKAILDEIEANGVPVKNREGSAEGGWVLIDLYDVLVHIFSEEKRQYY
                     SLEKLWQSAPKVES"
     gene            354..1046
                     /locus_tag="PLW82_02025"
     CDS             354..1046
                     /locus_tag="PLW82_02025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ46083.1"
                     /translation="MGKVFNAVYDQFMSQRPYTKYARCYLQACEAKTTDRILEAGCGS
                     GLFLMALNRLGYNPEGLDFDEAMLELAQKRVENKLYHGDIREFCSEEKWDVIFLPLDV
                     LNYFSRSGMKKAFKNLARNLNPHGVIVFDVHTKAHLKKVHGAQSRERLSDAQYIWRSR
                     GRGEYIHYYITCSKQGYHESYKLEQRVHSPREILRAAAYAGFRKKQRCRAWDLGRIDR
                     RTTRQLFVLQKV"
     gene            1064..2824
                     /locus_tag="PLW82_02030"
     CDS             1064..2824
                     /locus_tag="PLW82_02030"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOJ46084.1"
                     /translation="MNRYNYLLTERKWQERWKAQTAFDSEHKQKLIAGNTIPDSLPLH
                     MGHARGLVALDVLVRFYQMHGYSVSWSLGLPFLAFPEASEGSELVLSNLKQAQQSLLQ
                     IGLHCSFEDPEAVQRKVYEFSRWFFLAMWQNGLVYGRPSGDSCPFCGNVFSAEVVSKG
                     ICTQCGSSAFDWYADFSPYAETLQSDLLVSSQSDGMAVRPWRISGQSSVPIPLILCPT
                     CGVVAEDVVTRSSGSECGPDGSFGFVGDEALCPICGRVAAKETKTLDRSFVECITLLY
                     LAGSKEGIDGDYRNVPGSFDIFAGSISWARKDYLHLRSLTSLMHDGDEGCKIEPVKRL
                     VVVGHTKFSSEGAKRDADLQRMLKIYGADTCRWHLMFYAPLDRDITWSDEAIEGSYRF
                     ICRVWRLVHAVVVASEANKAKAVRANDEQENMLKGSLCRTAQEIAEITNGSTRLNFVC
                     SLLMTLANQIEEYMKSREGDLDLELLLDAASILVKMLYPFIPHAAAELWEVLGGSEEG
                     LVFGWPDLEMDFQCSRNIEIVVQINGKKRAMISVSSSASREEIEKAAIANPRIQQLIS
                     DSEVHKIVVVPGEVVNIVAG"
     gene            2914..3384
                     /locus_tag="PLW82_02035"
     CDS             2914..3384
                     /locus_tag="PLW82_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ46085.1"
                     /translation="MVLTGREKRFLALISLVITLFSVCELGLTRYRITLAKSLEDEIE
                     VGVVLTSERSMADEQDSNLFGEGEPKGEPTDESAAVTKAEKEEEVDEKKPKLNINTAT
                     FEELLDLPGIGKVLAQRIIEYRTEKGKFLALDELMEVRGIGEKLLERIKPYLTI"
     gene            3419..>5463
                     /locus_tag="PLW82_02040"
     CDS             3419..>5463
                     /locus_tag="PLW82_02040"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOJ46086.1"
                     /translation="MFGIILGFHSSLPKVTYVLVLTCSILGYFLALRIPSSCYWILLI
                     LAGALLAQPIKIHGGMLENYTKVDLCFYVEEVKGSSDEGYKARILSEEGRFYLYSNIP
                     IYPGEVMQVRTELRPVSPARNPGEFCFHTYLKGQGLRGTADLSFDWQVIKRKGSLKSG
                     FYRLLYSMRDRGKTRFSGLDHGSLYSGLVFGTSIDDDLVVPSREAGTSHFLVVSGLHV
                     GYMAKLVGSLPLFGFEKVAIVPFLIFYAFLAGGTPSVWRAVLAYFVAKEFKSRMLDRV
                     AFTLFFMLLFHPSWLFSASFQYSALAVFGISLTENWPIWGPLRISLGASFMVLPLSLL
                     KNRKMNPFGVPLSIVLGPLIAVMMVLSLVYLVLPVGLLRSVLDCLGLLFLRVNEAAAR
                     FTFPVSGFNRTEAVLFWALLLFFWHEGRILPQKRLLLRRAGAAVLIVCAYFSAGFLLE
                     AAKPVELYFFQILDGDAALVRLPYGRGVLIDTGSKIDTFDGAERILNPALLSLGVRRV
                     DTVYLTHRHLDHVGGLDNLVFCDKIMIGNELWDQKAALMEKAPVYLAQDVVFPCGTKV
                     KVVDLGRPGQDPNDNSLVQLVEVLGWRILFTADREMDGLEVLRELAIPADVVKVPHHG
                     SPGSLHLDWMKTTKASVAIFTGGKYGRPDPAVVEAWQRLSAETFVTKTGGAVRIRFYP
                     "
BASE COUNT         1329 a         1199 c         1525 g         1410 t
ORIGIN      
        1 aaggaggcgt gctggtggaa gccaaaaaga ttgtggattt ggtagtacga gcagcggaag
       61 aacacaaagg aatagacttg gagattttgg atgtgtctca gctcactatc gttgccgact
      121 actttgtcgt agtctcagga cgttctacgc tgcatgtaga cgcattaact aaagcaattc
      181 tagatgaaat agaagcaaac ggcgttcccg tgaaaaatcg agagggcagt gcagaaggcg
      241 gctgggtatt gatcgacctt tacgatgtac ttgtgcatat attttctgaa gaaaagcggc
      301 agtactacag ccttgagaaa ctctggcaaa gtgctccgaa ggtggagagt taagtgggta
      361 aagtcttcaa tgcggtttat gatcagttca tgtctcaaag accatacact aagtacgcca
      421 ggtgctatct acaggcctgt gaagctaaaa caacagatag gatcttggag gccggctgtg
      481 gttccggcct ttttctcatg gcactgaatc gcttgggcta caaccctgaa ggtttggatt
      541 tcgatgaggc tatgttggaa cttgctcaaa aaagagtaga gaacaagctc taccatggag
      601 atatcaggga gttctgtagt gaagaaaagt gggatgtcat ctttcttcct ctggatgtat
      661 tgaactactt cagcaggtcc ggtatgaaaa aggcatttaa gaatctagca agaaacctca
      721 atccccacgg tgtgatcgtc tttgacgttc acaccaaagc ccatttgaag aaggttcacg
      781 gtgcgcaaag cagggagaga ttaagcgacg ctcagtatat ctggagaagt cgagggcggg
      841 gagagtacat ccactactat ataacttgca gcaagcaagg ctatcacgaa agctacaagc
      901 tagagcaaag ggttcacagc cctagggaga tccttcgtgc tgcggcctat gcaggcttca
      961 gaaaaaagca aaggtgcaga gcatgggatt tgggaagaat tgaccgacgc acaaccagac
     1021 aacttttcgt tttacaaaag gtttagaaga ggtgcaggca atcatgaatc gttacaacta
     1081 tctgctcaca gaaagaaagt ggcaggagag gtggaaggcg cagacagcat ttgactccga
     1141 acacaagcaa aagctgatcg caggcaacac gattcccgat tccctgccgc tacatatggg
     1201 tcatgccaga ggccttgttg cactggatgt tctcgttagg ttttatcaaa tgcacgggta
     1261 tagcgtatca tggtctcttg gcctgccttt tcttgccttt ccagaagcga gtgaaggcag
     1321 tgaacttgtg ctgtcaaacc tgaagcaagc gcagcagagt ctgcttcaga tcggacttca
     1381 ctgcagcttt gaagacccgg aggctgttca acggaaggtg tacgagtttt ctcggtggtt
     1441 ctttttagcg atgtggcaaa atggtctcgt gtatggcagg ccgtctggag attcttgccc
     1501 gttctgcggc aacgttttca gcgctgaagt cgtatcaaag ggcatctgca ctcagtgtgg
     1561 cagctccgct tttgattggt atgcagactt cagcccgtac gctgaaactc tacagagcga
     1621 cctcttggta tcttcacaaa gcgatggtat ggcagttcga ccttggcgca tttctggtca
     1681 gagcagcgtt cccattcccc tgatcttgtg ccctacttgc ggcgtggtgg cagaagatgt
     1741 tgtcacccgc tcatctggct cggagtgcgg gcccgacggg tcttttggct ttgtcggaga
     1801 cgaggctctc tgcccaatat gcggaagggt agctgccaaa gagacaaaga ctctggaccg
     1861 gtcatttgtg gagtgcatta ctctcctcta tcttgcaggg agtaaggaag gtattgacgg
     1921 agattaccgg aatgtgccag gcagcttcga catttttgca ggcagcatca gttgggccag
     1981 gaaagactac cttcatctca ggtctcttac ttcactcatg catgatgggg acgaaggctg
     2041 caaaatcgaa ccggtgaaaa ggctagtggt agtgggacac accaagttca gctcggaagg
     2101 tgccaaaagg gatgcagacc ttcagaggat gctgaagata tacggtgctg acacatgccg
     2161 atggcacctt atgttctatg caccgctgga tagggacatt acttggagcg atgaggccat
     2221 cgaaggttcg taccgcttca tctgcagggt ttggcgtctg gtacacgcag tggtggttgc
     2281 ttccgaagcc aacaaggcca aagcagtcag agcgaacgac gagcaggaaa acatgttgaa
     2341 aggatctctg tgccgaacag cacaagagat tgcagagatc acaaacggca gcacccgtct
     2401 gaacttcgtc tgcagcctcc tgatgacctt ggcaaaccaa atcgaggagt acatgaagag
     2461 cagagaagga gatctggatc tggagctgct tcttgatgct gcttccatcc tggtaaagat
     2521 gctttatccg tttatccccc atgcggcagc ggagctttgg gaagtgctcg gaggtagcga
     2581 agaggggctg gttttcggct ggccggattt ggagatggat tttcagtgtt ccaggaacat
     2641 cgagatcgtg gttcagataa acggaaaaaa gcgagcaatg atttcggtgt cttcgagcgc
     2701 cagccgagaa gagattgaaa aggcggctat agcgaatccg cgaatacagc aactcatttc
     2761 ggacagtgag gtgcataaga tagtggtggt tccaggcgag gttgttaata ttgtagcagg
     2821 ataaagacaa ctcttgcaga aaactaaaag tgcaacaggg agtttcatca gtgagatgaa
     2881 gcttcctttt tttattatct ggaggggttt tgcatggttt taacaggtcg ggagaaaagg
     2941 tttctcgcac tgatatcgct tgtgattact ctgttttccg tctgtgagtt aggtctaaca
     3001 agataccgca tcactctggc caaaagccta gaggacgaga ttgaggtggg agtagtcctt
     3061 acaagtgaga gaagcatggc agacgaacag gactcgaatc ttttcggaga aggagaaccg
     3121 aagggagagc caactgacga aagcgctgca gtgaccaagg cagagaaaga ggaagaagtt
     3181 gatgaaaaga aacccaagtt gaacattaac actgcaactt ttgaagagct tctggatctg
     3241 ccgggcatcg gtaaagtttt ggctcagcga attatcgagt accgcactga aaagggtaag
     3301 tttctcgcgt tagatgaact tatggaggta cggggtattg gagagaaact gctggaaagg
     3361 attaagccgt atcttacaat ctaatctggg tttctggcct ttggcttgcg tatgtcttat
     3421 gttcgggata atcttaggtt ttcacagttc tctaccgaag gtcacgtatg ttttggtgct
     3481 tacctgcagc atactgggat atttcctcgc tctgcggatt ccatcaagct gttactggat
     3541 tctcctgatt ctcgcaggag cgcttcttgc acagcccatc aaaattcacg gagggatgct
     3601 ggagaattac acaaaagttg acctgtgctt ttacgttgag gaggtcaaag gttcttctga
     3661 tgaagggtac aaagcgagaa ttctctcgga agaagggagg ttttatctct acagcaacat
     3721 tcccatctac cccggcgaag tcatgcaggt gcgcaccgaa cttagaccgg tatctcctgc
     3781 gcgaaatcca ggagagttct gcttccacac ctatctcaaa gggcaggggc tcagaggcac
     3841 cgccgacttg agcttcgatt ggcaggtgat taagcgcaaa ggaagcttga agagcggttt
     3901 ttaccgattg ctgtactcta tgagagatag gggcaagacg cgcttttcgg ggcttgatca
     3961 cggcagtcta tacagtgggc tggtttttgg caccagtatc gacgatgacc ttgtagtacc
     4021 atctcgggag gcaggtacct cccacttcct agtggtctca gggctccacg tgggctacat
     4081 ggcaaaactt gtgggaagcc ttcccctttt cggctttgag aaggtagcaa tagttccatt
     4141 tctgatcttc tacgcctttc ttgctggagg gactccctct gtatggaggg cggtgttggc
     4201 ttacttcgtc gccaaggagt ttaaatcgcg aatgctggat cgggtagctt tcactctgtt
     4261 ctttatgctt ctttttcatc cgtcttggct tttttcggct tcgttccagt acagcgctct
     4321 tgcggttttt ggcatatctc ttacagaaaa ctggcctatc tggggtccgt taaggataag
     4381 tctgggagcg tccttcatgg tcttgcctct atcgctcctg aaaaatcgta agatgaatcc
     4441 gttcggtgtg cccctcagca tagtgctggg gcctttgatc gccgttatga tggtgctgtc
     4501 tctggtttat cttgtactac ctgttggact tctccgcagc gttttggatt gcttgggact
     4561 gttatttctc cgtgtaaatg aagctgctgc aaggtttacc tttcccgttt ccggtttcaa
     4621 taggacggag gcggtgctgt tttgggcgct tttgctgttt ttctggcatg aaggccgcat
     4681 actgcctcaa aaaaggttgc ttttgcgcag agcaggtgca gcagttctca ttgtttgtgc
     4741 ttacttcagt gccggattct tgctggaagc ggcaaagccc gttgaactgt acttctttca
     4801 gattttagat ggcgatgccg ctctggtccg cttgccgtat gggcggggcg tccttatcga
     4861 cacaggttct aagatcgata cctttgacgg agcagaaagg attctgaacc cggcccttct
     4921 ttccctggga gttcgccgag tcgacacggt ctatctgacc catcgccatt tggaccatgt
     4981 aggtggttta gacaatctgg tcttctgtga taagataatg ataggtaatg agctttggga
     5041 tcaaaaggcc gcgcttatgg aaaaagcccc tgtgtacttg gcacaggacg tggtcttccc
     5101 ttgcgggacc aaggttaagg tagtagatct gggaagaccc ggccaggatc cgaacgacaa
     5161 ctcgctggtg cagctagttg aggttttggg gtggaggatt ctctttaccg ccgacagaga
     5221 gatggacggg ctggaggttc tccgggaact ggccattcct gcagatgtgg tcaaggtgcc
     5281 tcatcacggg tccccagggt cgctgcacct tgattggatg aagactacta aggcttcggt
     5341 tgccattttt actggaggca agtacggacg acccgaccct gcggtggtgg aagcttggca
     5401 aaggctctct gcagagacct ttgtcaccaa aacaggtgga gctgtgagga tccgcttcta
     5461 tcc
//
LOCUS       DAOSDA010000052         5448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9465_length_5448_cov_3.287780, whole genome shotgun sequence.
ACCESSION   DAOSDA010000052 DAOSDA010000000
VERSION     DAOSDA010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9465_length_5448_cov_3.287780"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLW82_02045"
     CDS             <1..619
                     /locus_tag="PLW82_02045"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOJ46087.1"
                     /translation="YCVGFDTAVHNAVEAIHNVRATCDSHKEDIVVEVMGRDSGWIAA
                     VAGLTAGAEFVFVPEMNFDANLLVDKLKARHAQGFLSNIIVIAEGVTEFAHAKIESGV
                     DAFGNKMLSGIGFGLRDYLQEKLGKKTRAIVLSYIQRGGRPTSYEVFMDLNFGRTAVN
                     LLVDGKTNLMVAYKQGTLQPVDLKLARGKQPVREDILKMVKELTL"
     gene            735..1694
                     /locus_tag="PLW82_02050"
     CDS             735..1694
                     /locus_tag="PLW82_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOJ46088.1"
                     /translation="MDMNHALILAGGLGQRLWPISRKQSATKEGVDIHSQTMLELTIS
                     RVSQWVPRQRIWVVSSRSIETYGCRLIKEPFRKNTAAAIAWGLAQILHTDKEAFVGIF
                     PVDHLISPWEQVTAALEKAKRAGTITALGIKQSKEEASKQYGYIKMEEGLELGKVVDF
                     AEKPTRPEEYIEAGYLVNSGIYAAPARKLLLEIGRFLPSHFAAARARDVAAFARLRPI
                     SIDKGVMEKTDRLFAYPMKVLWEDIGTWKNYLKYAGEKGSFVKFNSDSSLVYSEGDLV
                     VLSGVQDTVVVVKDGIVFVMHKDAEKDMGKVLEEVRRSYPGFA"
     gene            complement(1789..2283)
                     /locus_tag="PLW82_02055"
     CDS             complement(1789..2283)
                     /locus_tag="PLW82_02055"
                     /inference="COORDINATES: protein motif:HMM:NF020022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ46089.1"
                     /translation="MTKKTTLYILCGLPAAGKSTLCQLRQGRVITADIIRKGIVGSLG
                     DSDHDEFVWETALNAAEYFLQFGDVYYDATNSTRARRKPFVDKAKELGKDVVCVWVAT
                     PLEVCIKQNQRRAKSNPDSPPDWVIEKVAGVFEPPTLDEGFAKIEKWLPAPSGMRAQF
                     IYKT"
     gene            complement(2280..3053)
                     /locus_tag="PLW82_02060"
     CDS             complement(2280..3053)
                     /locus_tag="PLW82_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ46090.1"
                     /translation="MATVIGLILLFLLFIVWGFVTANSLRYKMITIRAEGWPEKSTRI
                     LFMSDLHTSGRGFRIKRTIKLAKEAPCDIVCITGDIIVRRVRQEERVVQALKEIANGR
                     PTFLVWGNNDYNPEIDRAKLYEELQKANINVLVNERVKVGNLTIGGLGDPFWGKDDIE
                     KTLAEDVDLLLSHTPSPIFEVKKRAKLMLAGHTHAGQFCSIDGTPLMPELRKKEAFAY
                     GLSYHNGTHIYTTSGIGTTRLPLRFFCHPELVLLEVRPK"
     gene            complement(3041..4147)
                     /locus_tag="PLW82_02065"
     CDS             complement(3041..4147)
                     /locus_tag="PLW82_02065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ46091.1"
                     /translation="MQLLIWLLLFFAVYPYAFYPLILKLISLLRASCVPKDLYSEENL
                     PKATLLITAYNEEEFLEAKIQNSLKLDYPDLEIVVVSDGSTDRTNEILASFPEVKSVI
                     LPKRSGKPTALKAGLEVAGGEIICLSDVSALYEENCLKLLVAPLLSPEVGATGGVLSF
                     RKHNAATPGEVTYSSYESKLRALETEALGTIVLPGTFYAVRRRDMVLPPEDIIADDFY
                     VTCSLLERGLKLIQVDKAMAFEQASHDTSGEFIRKSRIIAGGMQTLARFPKLLFGKWG
                     FFLVSHKVLRWGAALFGGMHYLLLAFSGLCPWLFAAESVLLLLGIISTFLDLAGVKTP
                     KIMALLRHFVVVNAAVYWGIIGLFSGRQTVKWQR"
     gene            complement(4158..5333)
                     /locus_tag="PLW82_02070"
     CDS             complement(4158..5333)
                     /locus_tag="PLW82_02070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ46092.1"
                     /translation="MEKKQILITTIGKFPHVGGKSVHIETLTRSLPKASNGKFSPKVL
                     SFSDLGPFVQTFCSLPRVFKDIPLDFVLRRYFLKKHLGKEILSQVPLFLVQESSSFQL
                     AEYAGKPFILTVHGSSCDEYASSKAIPFGGRSYKKLLQEEIEAAKRAAFVITVDTNLK
                     NQILERSHIDGSKVTVLPNTVDTDVFSPEMNKKAQKEALGLNPSDFVTVVVRRLVPKN
                     GVQFAILALAKLQQKNVKLVVAGDGPERKSLEELVAQLHLEDQVVFLGAIQHHRTPEL
                     LRCADLCLIPSVPDQNVVEATSISALEAMATALPVVASGIGGLKELLEGGAGVLVPPA
                     DPEALAAAWKNIIDNPVFGESLGVKARAKVLQEYSLDKWIGTYIDLIERTIEASTGR"
BASE COUNT         1396 a         1322 c         1403 g         1327 t
ORIGIN      
        1 ctactgtgtt ggctttgata cagctgtcca caacgcagtt gaagccattc acaacgtcag
       61 ggccacctgc gattctcaca aagaagatat cgtggtcgag gttatgggca gagacagcgg
      121 gtggattgct gcagttgcag gcctaacggc aggtgcagag ttcgtcttcg taccggagat
      181 gaactttgat gcaaatcttc ttgtggacaa gctcaaagcc cgccacgctc aaggttttct
      241 cagcaacatt attgtgatcg cagaaggagt aaccgaattt gcccacgcca agattgagtc
      301 cggcgtggac gccttcggca acaaaatgct gagcggcatc ggctttggcc tgagagacta
      361 ccttcaggaa aaactgggca agaagactag ggcgatcgtt cttagctaca tccagcgggg
      421 tggacgcccc acatcctacg aagttttcat ggacctgaac ttcggcagaa ctgcggttaa
      481 cctgcttgtc gacggaaaaa ccaatctgat ggtagcctac aaacagggaa ctctgcagcc
      541 tgtggatctg aagcttgccc ggggcaagca gcctgtgcgt gaagatattc tcaagatggt
      601 aaaggaatta accctttaat aatgtaagta acagttcaag cgacatctcg cttgaactgt
      661 ttttttaagt actgattatg aagctctgca aagccttacc gctcaggcgg aggcaagggg
      721 ctttagagat ggggatggat atgaatcacg cactgatttt agccggaggg ctgggtcaaa
      781 gactctggcc tattagtagg aagcaatctg caaccaaaga gggagtagac attcactccc
      841 aaacgatgct ggaactaacc atatcccggg tctcccagtg ggttccgaga cagcgcatct
      901 gggtggtttc ttccagaagc atcgaaactt atgggtgccg gctaatcaaa gagccgttta
      961 gaaaaaacac agctgcagct attgcctggg gtctggcaca gatactgcat actgataaag
     1021 aggctttcgt ggggattttc cctgtggatc acctgatttc tccttgggaa caggtcacag
     1081 cggctttgga aaaagcaaaa agagcaggaa ctattacggc tctcggtatc aagcaatcca
     1141 aggaagaagc ctcaaagcag tacggttaca tcaagatgga ggaaggcttg gaactgggca
     1201 aagtggtgga ctttgcagag aaacctaccc gtcctgaaga gtatatagaa gcagggtatc
     1261 tggtaaacag cgggatctac gcggcaccag cgcggaaact acttctggaa attggaaggt
     1321 ttcttccaag ccactttgcc gccgctaggg ctcgagatgt ggcggcattt gccagactca
     1381 gaccgatatc tatagataag ggagtcatgg aaaagacgga ccggcttttt gcatatccga
     1441 tgaaagttct ttgggaagac atcggcacgt ggaaaaacta cctgaagtac gcaggggaga
     1501 aagggagctt tgttaagttc aactcagaca gcagtctggt gtactccgaa ggcgatttgg
     1561 tggtgctgtc gggagtacaa gacacggtgg ttgtggtaaa agacggtatc gtttttgtca
     1621 tgcacaaaga cgctgaaaag gacatgggca aggttctaga agaggtccgc agaagctatc
     1681 caggctttgc ctgatctgat gtgcagagtt tatggcaagc cagaaaaaca gggttcaagc
     1741 agaaaacaaa cagggtgcag cccacgatcg tttccagctg caccctgttt atgttttgta
     1801 gatgaactga gcccgcatcc ctgagggcgc aggcagccat ttttcgatct tggcaaaccc
     1861 ttcgtccagt gtgggaggtt cgaagactcc cgcgaccttc tctatgaccc agtctggagg
     1921 tgagtcggga ttggacttcg cccttctttg gttctgcttg atgcaaacct caaggggagt
     1981 tgcaacccac acgcacacaa catctttccc aagctcctta gccttgtcca caaacggctt
     2041 tctccgcgct cttgtggagt ttgttgcatc gtagtaaacg tcgccaaatt ggaggaagta
     2101 ctcggcagca tttagagcgg tctcccagac gaactcatcg tgatcagagt cgccgagact
     2161 ccccacaatg ccctttctga taatgtctgc ggtaatcacc cggccctggc gaagctggca
     2221 taaagtgctc tttcctgctg caggcagacc gcaaagaatg tatagagtag tcttctttgt
     2281 catttaggcc tgacctccaa gagcaccagt tcgggatggc agaagaaccg gagggggagt
     2341 ctggtggtgc cgatgcccga tgtggtgtag atgtgggtgc cgttatggta ggagagaccg
     2401 taggcaaaag cttctttctt ccgcagctcg ggcattagag gcgtgccgtc aatactgcag
     2461 aactggcctg cgtgggtgtg tcctgccagc atcaacttag cccgcttttt cacttcaaag
     2521 atgggcgaag gcgtatggga cagcaggaga tccacatctt cagcaagggt tttttcaata
     2581 tcgtcttttc cccaaaaggg gtcgcccaag ccaccgatag ttaggttgcc aaccttaacc
     2641 cgctcgttta ccagcacatt gatgttggcc ttttgcagct cttcgtagag ctttgcccta
     2701 tcgatttccg ggttgtagtc gttgttaccc caaaccagaa acgtcggtct gccatttgca
     2761 atctctttta aagcctgcac aacccgctct tcttgccgca cccgccgcac gatgatgtcg
     2821 cctgtgatgc agacaatatc gcagggagct tccttggcca gcttgatcgt gcgttttatt
     2881 cgaaaacctc tgcccgaagt atgcaggtcg gacataaaga ggatgcgggt ggacttttcc
     2941 ggccagcctt cggcgcggat ggtgatcatt ttgtacctta gagaattagc cgtaacaaat
     3001 ccccaaacaa tgaacaagag gaaaagcagt atgagtccga ttaccgttgc cattttaccg
     3061 tctgcctccc actaaatagt ccaatgatac cccaatacac tgctgcgttt acaacgacaa
     3121 agtgcctgag aagcgccatg attttgggcg tcttaactcc tgccaaatcc aagaaagtac
     3181 taatgattcc caaaagcaaa agtacgctct ctgcggcaaa gagccaaggg cagagaccgg
     3241 agaaggccaa aaggaggtag tgcatgcccc cgaaaagagc agcaccccac cgcaagacct
     3301 tgtgggaaac taaaaagaag ccccatttgc caaaaagcag tttcggaaaa cgggctagag
     3361 tctgcatacc gccggcaatg attctggact tgcggataaa ctctcccgat gtgtcgtggc
     3421 ttgcctgttc gaaagccatg gctttgtcca cttggatcaa cttaagccct ctttccagaa
     3481 ggctgcaggt gacgtaaaag tcgtcggcaa tgatgtcttc cggagggagt accatgtccc
     3541 tgcgccgtac ggcgtagaac gtgccgggca gcacaatggt gcccagagct tcagtttcca
     3601 atgctcgaag cttactctca taactgctgt aggttacctc gccaggagtg gctgcattat
     3661 gctttctgaa ggaaagaacg ccacctgtag ccccaacctc cggggaaaga agcggtgcaa
     3721 caagaagctt caagcagttt tcttcataga gtgccgatac atcggaaagg cagatgatct
     3781 cgcctccggc aacctctaga cctgccttaa gagctgttgg cttgccgctg cgcttaggga
     3841 gaattaccga ttttacctct gggaatgacg ccagaatctc attggttcta tcagtagagc
     3901 cgtcggagac gacaacgatc tccagatcgg gatagtcgag cttcagtgag ttctggatct
     3961 ttgcttcaag aaactcttct tcgttgtaag cggtgataag aagtgttgct ttaggcaaat
     4021 tttcttctga gtagaggtct ttcggaacgc aggaggccct cagcaacgaa atcagcttca
     4081 aaatcagcgg atagaaggca tagggataca cagcgaaaaa aagcaaaagc caaatcaaga
     4141 gctgcattca tctatcccta ccttcctgta cttgcttcga ttgttctttc tataagatcg
     4201 atatacgtac ctatccattt atccaaagaa tactcctgta aaaccttcgc tctggctttt
     4261 acacctaaag attccccaaa gacggggttg tcgattatgt ttttccaagc agctgccagt
     4321 gcttcggggt ctgcaggtgg aacgaggact cccgcacctc cttctagaag ctctttcaac
     4381 cctccgatgc cgctggcaac aactggaagt gccgtcgcca tggcttcgag ggcagagata
     4441 gaagtggctt ccacgacatt ttggtcggga accgaaggaa tgaggcagag atctgcacac
     4501 ctgaggagct ccggagttct gtggtgttga atggcgccga ggaaaactac ttgatcttct
     4561 agatgaagct gagctaccag ctcttctaag gactttcttt ccgggccgtc gcctgccaca
     4621 accagcttga cgtttttctg ctggagtttt gccagagcca aaatggcgaa ctgtacgccg
     4681 tttttcggta ctagcctgcg aacaactacc gtaacaaaat cgcttgggtt tagacccaag
     4741 gcttcttttt gtgctttttt gttcatttct ggagagaaaa cgtcagtgtc aacggtgtta
     4801 ggcagtaccg tgactttact accatctata tgagaacgct cgagaatttg attcttgaga
     4861 ttggtatcga cggtgatcac aaaggcagct cgcttggccg cttcgatctc ttcttggagg
     4921 agctttttgt agcttctacc gccaaaaggt atggccttac tgcttgcata ctcgtcacag
     4981 cttgaaccgt gaacagttag aataaacggc tttcctgcat actctgcaag ctggaaagac
     5041 gagctttcct gcactagaaa gagcggcacc tgcgacagaa tctctttacc caaatgcttt
     5101 ttgagaaagt accttcttag gacaaaatcc agcggaatat ccttaaagac tctgggcaga
     5161 gagcaaaagg tctgcacgaa aggcccgaga tcggaaaagg agagcacttt agggctaaac
     5221 ttcccatttg atgccttagg cagcgatctg gttagagttt caatgtggac cgactttccg
     5281 ccaacatggg ggaattttcc aattgtggtt atgaggattt gctttttctc cacttagtcc
     5341 acgctacttt caaaccttta ataaatccga tcatgtccgc aaaatctatg gtgaaccgca
     5401 atgggagggt gtaaatactc agacacttgc tgaagtcttt ggccagac
//
LOCUS       DAOSDA010000053         5439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9486_length_5439_cov_2.820579, whole genome shotgun sequence.
ACCESSION   DAOSDA010000053 DAOSDA010000000
VERSION     DAOSDA010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9486_length_5439_cov_2.820579"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /gene="pyrG"
                     /locus_tag="PLW82_02075"
     CDS             <1..1131
                     /gene="pyrG"
                     /locus_tag="PLW82_02075"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HOJ46093.1"
                     /translation="AIRQMKSDVGRDNCLYIHVTLVPYIKSAEELKTKPTQHSVKELR
                     SIGIQPDVIVCRSDRPLGHKIREKIALFCDIDKDAVIPNLDAETIYDVPLIFAEEGLD
                     EIVIKKLQLNCSDHDLIEWRQMVEKIKNPKHAVRIAIVGKYVALPDAYLSVVEALKHG
                     GIANDTHVEIKWIDSEKLEEDGFINEALADVDGILVPGGFGHRGVEGKILAAKYAREH
                     KLPYLGICLGMQTAIIEYSRNVLGFAGANSSEFDTNTPYPVIDLLPDQDPAKLGGTMR
                     LGNYPLEVVPGTKLAEIYNNQNVLERHRHRYEVNPNFVEQLKAAGLVVSGVWKEGHLV
                     EAIELKDHPWFVATQYHPEFKSRPTSPHPLFASFIAAALGSK"
     gene            1228..1752
                     /locus_tag="PLW82_02080"
     CDS             1228..1752
                     /locus_tag="PLW82_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46094.1"
                     /translation="MKTSLKLVTFAIAAMFLLCIDGSAQTETSSVTKAILTGVVTEGG
                     SIYLRYRTPMEELVISEVTEGTKILLEDVEISAEQLKTLVESDEFSAWIWLSGRQATK
                     VMLAKNVYQIRIKAIDPALKRLCSVDGRWYYVEHDVAIHRLVAGELVNVHLSFDGTLA
                     KIVPAEPSIAKDKM"
     gene            2173..3231
                     /locus_tag="PLW82_02085"
     CDS             2173..3231
                     /locus_tag="PLW82_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOJ46095.1"
                     /translation="MPDKLRPKGLLIVGIALIILLGSGSYLVVRAFLGYPLFWENNIG
                     GGNEEDLLYLLDNMDYLASDTSDFVADTARISYTPPAASAAERIEDAVPEVSQAAEEE
                     EPVQTPLSAWDLVKVHVVEKGESLWSIARKYDIDVDTIIGSNELKSYDTINIGQKLTI
                     LPFKGVTHKVEKGDSLWSIAQRYGASVDEIIETNELVSQNIQVGQTLLIPGGKLSEAE
                     KERRLLIARGGIPEFIRPVSGGWISSRFGMRWGKMHQGVDLAVSTGTPVKAAASGTVT
                     QAGNAGSYGLLVIIKHANGYETRYAHNSRITVKVGQYVKQGDIIAYSGNTGNSTGPHL
                     HFEIRVNGKAVDPLNFIK"
     gene            complement(3417..4688)
                     /locus_tag="PLW82_02090"
     CDS             complement(3417..4688)
                     /locus_tag="PLW82_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ46096.1"
                     /translation="MPKKSSISFDATSLTYNMVYMNADGEFIDSELPAAVRSGNEFFI
                     THDFIPLPEGSSLIYLPGRLPVAYVDEEFLSVESPDEDIYPLCALLPAGYTRLFLPAY
                     ENSVDAPILPLFGYAAVAIKDGEFFVAAKRTDDPVKWNPLNYPQDKLEEGVSYLKEEM
                     PENRLVEHLAHCALEYHCLTASNIFLNRWEGGIPTSPTCNAGCLGCISLQESECCPSP
                     QERIAFRPTPEEIAEIAIYHLEKGDEPIISFGQGCEGEPLLASSTIKKAIEKIRMTTK
                     KGIINMNTNAFSKDRLLSLREAGLDSIRISMISNIEETYNLYHNPCGYSLNDVRTAGR
                     AAKSAGLFISLNLLTIPGLNDAPTELRAWEELLAEGWVDLVQIRNLNIDPDWLFAKIP
                     LKEKGLGILHFIDAISKYTKVGNFSHYELKK"
     gene            4800..5438
                     /locus_tag="PLW82_02095"
     CDS             4800..5438
                     /locus_tag="PLW82_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOJ46097.1"
                     /translation="MATKPFDYKKEYKELYQPKTIPQLVDVPEMNFIMVDGRGDPNDS
                     PLFQEAVEILYGISYTIKMSSKKGMEPEGYFDYVVPPLEGLWWIDGTEFSFTDRENWL
                     WTLMIRLPEFVTSDVFAWAKAETKKKKPELGVDKARFETFKEGLCVQIMHIGPYSTEP
                     ESMAKVKEFLEANGLVETFTEGGKHHEIYLSDPRKANPEKMKTVLRHPVRKV"
BASE COUNT         1470 a         1230 c         1420 g         1319 t
ORIGIN      
        1 gctatccggc agatgaaaag cgatgtaggt agggacaact gcctgtacat ccacgtaacc
       61 ttggttcctt acatcaaatc ggctgaagaa ctgaaaacca agccgacaca gcacagtgta
      121 aaagagctcc gcagcatcgg tatacagccc gatgtgatcg tctgccgctc tgacagacct
      181 ctcggtcata agatcagaga gaagatagcc ctattctgcg atatcgataa agatgcggtt
      241 attcccaacc tagatgcaga gaccatctat gatgttccgc tgatctttgc tgaagaaggg
      301 ctcgatgaga tcgttatcaa gaaactccag ctgaactgct ccgatcacga tctaatcgaa
      361 tggcgccaga tggtggagaa gattaaaaac cccaaacatg ccgtaaggat tgccatagtg
      421 ggcaagtacg tggcgctgcc agatgcctac ctgtcggttg ttgaagcttt gaaacacggt
      481 ggaattgcta atgacaccca cgtggagatc aagtggatag attcggagaa gctggaagaa
      541 gacggattca ttaacgaggc acttgccgac gtcgatggca tattggttcc aggtggtttt
      601 ggccaccggg gcgtagaagg taagattctg gctgccaaat acgccagaga gcacaaactg
      661 ccatatctcg gtatctgttt aggtatgcag actgccatta tcgaatacag ccgcaacgtt
      721 ctgggctttg ctggagcaaa cagctctgag ttcgacacca atactccgta tccggtgatc
      781 gatcttcttc ccgatcagga tcccgccaag ctaggcggca ccatgagatt gggcaactac
      841 cctctggagg ttgttccagg aacgaagctt gccgaaatct acaacaacca aaacgttttg
      901 gagcgccatc gccacaggta tgaggtcaat cccaactttg tggaacagct caaagctgca
      961 ggtcttgttg tcagcggtgt atggaaggaa gggcatctgg tcgaggccat cgagcttaag
     1021 gatcaccctt ggtttgttgc cacccagtac cacccagagt tcaagtcccg tccgacaagc
     1081 ccccatccgt tgtttgccag cttcattgct gcagctctag gaagcaagta gattaaaaaa
     1141 ctagaaaaaa ttgccttaga acgcgtataa ttttatgctt ccctacccag aatcagatta
     1201 gctgattcgt gagggaggca ttttttcatg aagacgtctc ttaaattggt aacttttgca
     1261 atagcagcga tgtttttact gtgcatagat ggaagtgccc aaactgaaac ttcttccgtg
     1321 accaaagcga tccttaccgg agtagtgaca gaaggcggat cgatctacct gcgatacagg
     1381 actcctatgg aggaactggt gatctccgaa gtcacagaag gaaccaaaat cctcctggaa
     1441 gatgtggaga tctctgcaga gcagctgaaa acccttgtgg aaagcgatga gttttctgcc
     1501 tggatttggc tctctgggag acaggcaact aaagtgatgc tggcaaaaaa tgtgtatcag
     1561 atcagaatca aggccatcga tccagctctg aagcggctct gctcggtaga tggaaggtgg
     1621 tactacgtcg agcacgacgt agcaatccac cgcctagttg caggggagct ggtgaacgtc
     1681 cacctgtcct tcgacgggac ccttgcaaaa atcgtaccgg cagaacctag cattgctaaa
     1741 gacaagatgt agctaaaata gccttgttta acagggctat tttttttgtg ccctaacttc
     1801 gcttttttgg cgagggtgca aagcgcgact agcgtacact ttaccttttt ggcattttgt
     1861 tatatagtca gaagagaact ggtttctatg ggagcaagag ccaagtagag ctggagaacg
     1921 aaggcttggg cgcatttgca gtacataggc agctcaatcg gcctcttttg cctttttgga
     1981 gcacgatttc aaggcagaag taaaccgtgg gaatatagaa gcagccgaac gccggaccct
     2041 gggccggcta cttaagacta gcatttactg acttgggtta tacttttttc agagggatgg
     2101 aaaggatatt ctgcgtgaaa agagaacact tactttatgg gggaaacaaa gaatacttag
     2161 gtgggatgac taatgccaga taaattaaga ccaaagggtc ttcttatcgt cggcattgcc
     2221 ctgattattc tccttggatc aggctcatat ttggtggtta gagccttttt gggctacccg
     2281 ctgttttggg agaataacat tggtgggggt aatgaagagg atcttctcta tttattggat
     2341 aacatggatt atttggcgtc cgatacatcc gattttgtcg ccgatactgc taggataagc
     2401 tacacgccac ctgcagcatc agctgcagag aggatagagg acgcagttcc cgaagtctcg
     2461 caagcagcgg aagaagagga acctgtacag acgccactct ccgcatggga tttggtaaaa
     2521 gtccacgtgg tggaaaaagg tgaaagcctt tggagcatcg ctaggaagta cgatatcgac
     2581 gtggacacca tcatcggttc taacgagctg aagagctatg ataccatcaa catcgggcag
     2641 aaactcacca tcctaccttt taagggtgtt actcataagg tggaaaaagg ggatagcctt
     2701 tggagcatag cccaacgtta tggggcatcc gtagacgaga ttattgaaac caacgaactt
     2761 gtaagccaaa acattcaagt cggtcagacg ctgcttattc caggtggcaa gctatcagag
     2821 gcagagaagg aaagacgact cctcattgca aggggtggta tccccgagtt catcagaccg
     2881 gtttccggcg gatggatctc ctctcgtttc ggtatgcgct ggggtaagat gcatcagggc
     2941 gtggatcttg cagttagcac aggtacgcct gtcaaagcag cagcttctgg aaccgttacg
     3001 caagctggaa atgccggaag ctacggtctt ctggtgatta ttaagcatgc caacggttat
     3061 gaaactcgct acgcccacaa cagccgtata acggtaaagg taggccagta cgtcaaacaa
     3121 ggcgatatca ttgcctactc cggtaacaca ggaaactcca caggtccgca ccttcacttt
     3181 gaaatcaggg tgaacggcaa agcggtggac ccgttgaact tcataaaata gcagtttagc
     3241 gtgctatgct tgcatagcac gcttttgctt tcttgtgtac aggtccatgt tttctaagag
     3301 gaggggaggc ctccggcgaa aacaaacaga gtaatagtac gaaaaaaagc agaccaccgc
     3361 aaggtggtct gtgggttttc taggggaact agcttgtagg agttgtttgg aaacagttac
     3421 ttcttcaact cgtagtgaga gaagttgccg actttggtgt acttggagat ggcatcaata
     3481 aagtgcaaaa tgccaagccc cttctcctta agagggattt ttgcaaagag ccaatcgggg
     3541 tcgatgttta ggttccggat ctgtaccaag tccacccagc cttctgcaag cagctcttcc
     3601 catgcacgaa gctcggtagg agcgtcgttc agacctggga tggttaaaag attcagagag
     3661 ataaacagcc cggcactctt tgcagctctt cccgccgtgc gcacgtcgtt taagctatat
     3721 ccgcaggggt tgtggtagag gttgtaggtc tcctcaatat tggagatcat actgatgcga
     3781 atagagtcca gacctgcttc tctaaggctt aataggcggt ctttagagaa ggcattggtg
     3841 ttcatgttga taatgccctt tttggtagtc atcctgatct tctcgatggc ttttttaatg
     3901 gtgcttgatg caaggagagg ctctccttcg caaccctgac cgaacgagat tatcggctca
     3961 tctccctttt ctagatggta gatggcgatc tcggcaatct cttcaggagt aggtctgaaa
     4021 gctattctct cctgaggtga cgggcagcac tccgactcct gcagggaaat gcagcccaga
     4081 cagcctgcat tacacgtagg agacgttgga attccgccct cccaacgatt gaggaagatg
     4141 ttcgaagcag taaggcagtg gtattctaaa gcgcagtgag caaggtgctc taccaagcgg
     4201 ttctccggca tttcctcttt aaggtaggac acaccttctt ctagcttgtc ctgggggtag
     4261 tttagcggat tccattttac tggatcatct gtcctctttg ctgctacgaa aaactcgccg
     4321 tccttgatag ccactgcagc gtagccaaag agcgggagga tgggagcatc tacagagttt
     4381 tcgtaagcag gtaggaagag cctggtgtat cctgcaggaa gaagagcgca gagagggtaa
     4441 atatcttcat cagggctttc aacagacaaa aattcttcgt ctacgtaggc aacgggcaac
     4501 cgtcccggaa gatagatcag gctactgcct tctgggaggg ggataaagtc gtgtgtaatg
     4561 aaaaactcat tcccacttcg taccgcagca ggcagctcgc tgtcgataaa ctcgccatct
     4621 gcgttcatgt aaaccatgtt gtatgtcaaa ctcgtggcgt caaaactaat actcgatttc
     4681 ttaggcataa ctctacctcc agagggtaat tgtaaaggag tagtgcatac ggtctctgta
     4741 aatagtatca atagtctggc tgctctggca agcacttcca aaattgagga gggaatcctg
     4801 tggctaccaa accgttcgac tacaaaaaag aatacaaaga actttaccag cccaaaacta
     4861 tcccacagct ggtggacgtt cccgagatga acttcatcat ggtagatgga aggggcgatc
     4921 ccaatgatag tcccttgttt caagaagcag tggagatcct gtacggcatc tcgtatacca
     4981 ttaagatgtc cagcaaaaaa ggcatggagc cggagggata ctttgattac gttgttccgc
     5041 ctctagaggg gctgtggtgg atagacggca cggagttttc ttttacagac agagaaaact
     5101 ggctgtggac cctaatgatc cgcctacctg agtttgtgac cagtgatgta ttcgcctggg
     5161 caaaggcaga gacgaaaaag aaaaagccgg agctgggtgt ggataaagcc agatttgaga
     5221 cgtttaaaga gggtttgtgc gttcagatca tgcacatcgg cccctacagc accgaacctg
     5281 agtcaatggc gaaggtgaag gaatttcttg aagccaacgg tttggtggag acctttacag
     5341 aaggtgggaa gcatcacgag atctacctca gcgatccccg aaaagcaaac ccagaaaaaa
     5401 tgaagaccgt tctgcgccat ccggtaagaa aagtttagg
//
LOCUS       DAOSDA010000054         5422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9524_length_5422_cov_3.238122, whole genome shotgun sequence.
ACCESSION   DAOSDA010000054 DAOSDA010000000
VERSION     DAOSDA010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9524_length_5422_cov_3.238122"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PLW82_02100"
     CDS             <1..938
                     /locus_tag="PLW82_02100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOJ46098.1"
                     /translation="ICNLETVLAGPDRGYSGYPDFNAPDEIAVAIKEAGFDVVSLANN
                     HSLDKGPSGIYRTIDYLRSIGLIPIGSIRSEEEKDDILLLEVGGISLAVLAYTYGVNS
                     PYPTENLVSIIDEEKIAGDIAKAKDMGAELVAVSIHWGTEYKREPNQAQKNLAEKMIE
                     FGADIILGSHPHVIQPFDKVEVALPSGETREGYVIYSLGNFISNQNLQTLNMKYVDSG
                     VILTFTIEKDPETGEISIPAIGYTPTWVHKFFKDGKDHYRIVIVEDAIKEYESGQPGD
                     LSEADYLRLKEVLEETREQFRSLDLELAVSTEQDE"
     gene            1186..2769
                     /gene="gpmI"
                     /locus_tag="PLW82_02105"
     CDS             1186..2769
                     /gene="gpmI"
                     /locus_tag="PLW82_02105"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOJ46099.1"
                     /translation="MQQKRPVVMVIMDGIGINNDDFGNAVKSAYMPTLEHLLGPCPNI
                     QIQAHGTAVGLPSDDDMGNSEVGHNALGCGQIYAQGAKLVNESIASGRLFTSQTWQTI
                     VTNCLEHNSTLHFIGLLSDGNVHSNIAHLKAMLIRAKEEGVKRARVHVLLDGRDVGAT
                     SALKYIEDIENLLSELNDGDFDGRIASGGGRMKITMDRYEANWEMVRLGWETHVLGIG
                     RQFPNATEAIETYRTELGVIDQDLPAFVVAEDGKPIGTIEDYDSVILFNFRGDRAIEL
                     SRAFEEEHFDKFDRVRYPKVIFAGMLQYDGDLKIPRRYLVTPPDIQHTLSEHLIKYNL
                     KQYAVSETQKFGHVTYFWNGNRIEKFDEVLEVFEEVPSDKVPFEERPWMKAAEITDKL
                     IEALESGKYSFLRANFPNGDMVGHTGNFAAAKIAAETVDLCLARIVKAVDRTGAILIV
                     TADHGNSEEMYEKKGADGSIKPKTSHTLNPVPFIIYDKKEKRELKDGKFGLANVAATI
                     TELLGLKKHEAWEESMLKS"
     misc_binding    3577..3759
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3812..4786
                     /gene="trpS"
                     /locus_tag="PLW82_02110"
     CDS             3812..4786
                     /gene="trpS"
                     /locus_tag="PLW82_02110"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOJ46100.1"
                     /translation="MAKVFSGIQPTGIIHIGNYLGAIKNWVELQEEHDCLFSIVDLHS
                     LTVPYDPKGLGDRIKDALATNIAAGLDPEKATIFVQSMVPEHTELTWLLTTITPVGNL
                     ERMTQYKEKSEKQGLLSGLLCYPILMAADILLYKADLVPVGEDQIQHLELTRVIARKF
                     NSTFGEYFPEPQPKLSKATRVMALNDPTSKMSKSIPGSYIALTDDPEEIRTKVKRAVT
                     ASDVEGEVSPGVANLFTLLEAFAPKDVYNKFAKDLEDGNLKFSELKPALAEAIVEELT
                     PIREKRQELLAKPKFLEEVVAAGADRARREAKQHMQEIKELMGLNLTF"
     gene            complement(4921..>5422)
                     /locus_tag="PLW82_02115"
     CDS             complement(4921..>5422)
                     /locus_tag="PLW82_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOJ46101.1"
                     /translation="DGYLIQTDAAINPGNSGGPLINIRGEVIGINTAVNSAAQGIGFA
                     IPINTAKKVVDQLIEHGRVIRPYLGIYYYSEISPAQAEALGLPDTHGILVGEVIKDGP
                     ADKAGMKARDFIRSINGKRVESDKDFEEILSNAKVGDELIVDVIRDGKYVSLKIVLGA
                     RPTTTQ"
BASE COUNT         1507 a         1281 c         1380 g         1254 t
ORIGIN      
        1 ccatctgcaa cttagagacg gtccttgcgg gaccggatag gggctactca ggatatccgg
       61 acttcaatgc tcctgacgaa attgctgtag ctatcaaaga ggcaggtttt gatgtggtgt
      121 ctttggcaaa caaccattct ctagataagg gaccgtcggg catctacagg actatcgatt
      181 acctaagaag cataggtctg atccccattg ggagcattag gtctgaagaa gagaaagacg
      241 acatcctgct tctggaagtt ggtgggatat ctcttgcggt tttggcctac acctatggtg
      301 tcaattcacc ctaccccaca gagaatcttg taagcatcat cgatgaggag aagatcgcag
      361 gagatatcgc aaaggctaaa gacatgggag ctgagctagt tgcagtcagc atccactggg
      421 gcaccgagta caagagagag cctaaccaag cgcagaagaa cctggcagag aagatgattg
      481 agtttggcgc cgacattatc ttgggaagcc atccccacgt catccagccc tttgataagg
      541 tcgaagtcgc tttaccctca ggagaaacta gagaggggta cgtcatttac tccctgggaa
      601 acttcatctc caaccagaac ctccagacgc tgaacatgaa gtatgtggac tcgggagtaa
      661 tactgacatt caccatcgaa aaagacccgg agacaggaga gatctccatt cccgcaatcg
      721 gctatacccc aacctgggtg cacaagttct ttaaagacgg aaaagaccac tacaggatcg
      781 tgatcgtgga agacgctatc aaagaatatg agtcgggaca gccaggcgac ctttctgaag
      841 cggactacct caggctgaaa gaggtgcttg aagaaaccag ggagcagttc cgcagccttg
      901 atttagagct agcagtctct acagagcagg atgaatagac ctagataggc agatggaacg
      961 tacttttacg gcagaacctc tgttgtggat atcctgttga cttgtggact aattggctct
     1021 gggttttggg tttaaactaa gtagcggcga tcagtggagt ttacaaacct ttctgcgtgg
     1081 tatatttaga tagtggaagt cctaatgtaa aggatcatgc gtagtacggt aaagtaaaca
     1141 ctttttgtgt acaaatagag atgaaaacga tttggaggct ttaaaatgca acaaaaaaga
     1201 ccagttgtta tggtgattat ggatggaatc ggaataaaca atgatgattt cgggaacgcg
     1261 gtgaaatcgg cgtacatgcc aacccttgag cacctccttg ggccctgccc caacatacag
     1321 atccaagccc acggtactgc tgtagggcta ccgtctgatg acgatatggg aaacagcgaa
     1381 gttggacata acgcccttgg ctgcgggcag atctacgctc aaggcgcaaa gctggttaac
     1441 gagtccatcg cgtccggtag gctgtttacc tcgcagacct ggcaaaccat cgtcacaaac
     1501 tgtctggaac ataactccac actgcacttc atcggtctgc tctccgatgg caacgttcac
     1561 tccaatattg cccacctcaa ggcaatgcta attagagcta aagaggaagg tgtcaagaga
     1621 gcccgtgtcc acgtgctcct agacggtcgc gacgtcggcg ccacctctgc cttgaagtac
     1681 atcgaagaca ttgagaacct gctttcagaa ctcaatgacg gagacttcga cggccgcatc
     1741 gcctccggcg gtggcagaat gaagatcact atggacaggt acgaggccaa ctgggagatg
     1801 gtaaggctgg gctgggagac ccacgttctg ggtatcggca gacagttccc aaatgccaca
     1861 gaggccattg agacttatag aacagaacta ggcgtcatcg accaagacct ccctgctttt
     1921 gtcgtcgctg aagacggaaa gcccatcggc accatcgaag actatgacag cgtgatcctc
     1981 ttcaacttca gaggcgatcg tgccatcgag ctcagtaggg cgtttgaaga agagcacttc
     2041 gataagttcg atagagtaag ataccccaag gtgatctttg caggaatgct gcagtatgac
     2101 ggcgatctga agatccccag acgctatctt gtaactcctc ctgacattca gcacactctc
     2161 tccgaacacc tgatcaaata caacctcaag cagtatgcgg tctcggaaac gcagaagttt
     2221 ggtcatgtca catacttctg gaatggcaac agaatcgaga agttcgatga ggtgctggag
     2281 gttttcgaag aagtaccatc ggataaggta ccctttgagg agcgtccgtg gatgaaggca
     2341 gctgaaatca cagacaagct gatcgaggct ctggagtccg ggaagtactc cttccttagg
     2401 gctaacttcc ccaacggcga tatggtaggg cacaccggaa actttgctgc agcgaaaatc
     2461 gcagcagaaa cagtagatct gtgccttgca agaatcgtca aggctgtaga tcgcacaggt
     2521 gcaattctga tcgtaaccgc cgatcacgga aactcagagg agatgtacga gaagaaggga
     2581 gccgacggca gcattaagcc aaaaacctcc cacaccttga acccggtgcc gtttatcatc
     2641 tacgacaaaa aagaaaagcg cgaactgaaa gacggcaagt tcggtctggc aaacgtagca
     2701 gctactatta cagaactttt gggattgaag aagcacgagg catgggagga aagcatgctg
     2761 aagtcataga tatttcgcag gagctcatgt aaattgcggt tcttgcacaa aaaggcaacc
     2821 ccgaccctga aaagggctag gggttgcttt gtattcagct accagatttt gtacaagtcg
     2881 tactgtcagt gctgaacagc aacgccttag gaaagcggtt aaacggtctg gtgttgctgt
     2941 agaagttgag tacacatgtg caaagaacaa agctctcaaa ggcaaaaacc ggagtatctc
     3001 gaaatgcgta ttaagtgcta gcgaacccgt tggccatcgc gataagtcgg tgatgctgct
     3061 atgcatattt acaggagtgt cgtctagtgc ttaacgacaa tgtcaataat gttcaagctt
     3121 tacggaacat tgaggcgctg atgaaatacg aaacaaggga aaaggtgagc atagaaatgc
     3181 acacaactat gcgttaccag ataacaaaac cgttgggcgg tcagcaggaa gaaccactta
     3241 cttgttctcc tgtcgcgaac tatctgggaa ccctttattt tataatcgtc cgaaataatc
     3301 ctattgagaa taactcgctg agtgttgtct ttcactgcaa gaacaagccg gaatcttcct
     3361 ctctgttttt ttcctagctc tttcaacctt tctctgctaa caagactcag ttcaacgttg
     3421 cttacaggtg tgagttggag gacagccata cctttcaccc tatattccgc ccttggttgc
     3481 aacttgtttg tctaaccgtg cgtcgatgac agctgcaatc ttgatttttc gcctgctgca
     3541 ggatataata atcgaagata accttgaatt ttgcttcaca aggctatgaa gggatgaagt
     3601 accaggttaa gcctgttaca gagagctggg gtagctgaaa accagcacag gatacctgcg
     3661 aaatgggccc cggagcttta agggagttgc cctgtacagc aacaaagagt ggccggattt
     3721 ggcaattagg gtggtaccgc gggacctctc gcccctttgt taggagcgag aggctttttt
     3781 attagcattt taagtggaga ggtgatttag aatggctaaa gtcttttcag gtattcaacc
     3841 cacaggcatt attcatatag gcaactactt aggggcgatc aagaattggg tagagctgca
     3901 ggaagaacac gactgcctct tttccatcgt agatctgcac tcccttacag tcccctacga
     3961 ccctaaaggg ctgggagata ggattaaaga tgctctggcc accaacattg cggcaggact
     4021 cgatccagaa aaggccacga tctttgtgca gtcgatggta ccggaacaca ctgagctgac
     4081 gtggctcctt actactatca ctccagtagg caatctggag cgcatgaccc agtacaaaga
     4141 gaagtccgag aagcagggac tactttcagg gctcctctgc tatcctattc ttatggcagc
     4201 agatattctg ctgtacaagg cagacctggt tccagtaggt gaagaccaga tccagcactt
     4261 ggagctgaca agggtcatcg ccaggaagtt caactctacc ttcggtgagt acttccccga
     4321 gccccagccc aagctgagta aagccaccag agtcatggct ctaaacgatc caaccagcaa
     4381 gatgtcaaag agcattccag ggagctacat cgccctcact gacgatccag aagagatcag
     4441 aacgaaggtc aagcgggcag ttaccgctag cgatgtagaa ggtgaagtca gcccaggtgt
     4501 agcaaacctg ttcacgctcc ttgaagcttt tgcaccaaaa gacgtctaca acaagtttgc
     4561 caaagaccta gaagatggca atctgaaatt ctccgaactg aaacccgctc tcgcagaggc
     4621 tattgttgaa gaactcacgc ccattagaga gaagcgtcag gaactcctgg caaaacccaa
     4681 gttcctggaa gaggtagttg cagcaggtgc tgaccgcgct agaagagaag caaaacagca
     4741 catgcaggag atcaaagaac ttatgggttt aaacctcacg ttctaagaaa aagcatacga
     4801 ggattcatta aggcttccca cagagcaaag ggaagccttt tagttatggt tgggcagggc
     4861 agaaaaagcg ggctctgtct agcaaagccc gcttggtaag ttcgatattt actttagcag
     4921 ttattgagtt gttgttggac gtgctcctag aacgattttc agcgagacat acttgccgtc
     4981 gcggataacg tccacgatca actcgtcacc gactttagcg tttgagagga tctcctcgaa
     5041 gtctttatcc gactcgaccc gtttgccgtt gatcgaacgg atgaagtccc gtgccttcat
     5101 gcctgcttta tctgcaggac cgtctttaat cacttcgcca actaggatac cgtgggtatc
     5161 gggaagacca agagcttctg cctgagcagg gctgatctcg ctgtagtagt agatacccag
     5221 gtaaggcctg atgactctac cgtgttcaat cagctgatct acaacttttt tcgccgtatt
     5281 gataggaatt gcaaatccga tgccttgagc ggccgagtta actgcagtgt tgatgccgat
     5341 aacctcacct ctgatgttga tcagcggtcc gccgctgttt ccggggttga tcgcagcatc
     5401 ggtctggatt aaatacccgt cg
//
LOCUS       DAOSDA010000055         5421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9529_length_5421_cov_2.828364, whole genome shotgun sequence.
ACCESSION   DAOSDA010000055 DAOSDA010000000
VERSION     DAOSDA010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9529_length_5421_cov_2.828364"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..901)
                     /locus_tag="PLW82_02120"
     CDS             complement(146..901)
                     /locus_tag="PLW82_02120"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOJ46102.1"
                     /translation="MKRLYIILVLLMLPLAVCALSPIPGTNVHDFAGILSNEHLLDSR
                     IEAMYQRTGVEMAVVTVPDLGNESIEMYAVKLFEDWGIGKKGEDKGLLLLISLNPREM
                     RIEVGYGLEGALPDSFVGVNLQKYVGPALANNDINGLIEFVAILEERIIAQGDAVPRV
                     AGETEGISRYETNDGPNPLAGLILFVIIVALVLLDFRFTGGMIVGAILHSLLRGGSYR
                     GGSYRGGSYRGSSGRNRYGGGGRSGGGGASGKW"
     gene            complement(951..1514)
                     /locus_tag="PLW82_02125"
     CDS             complement(951..1514)
                     /locus_tag="PLW82_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOJ46103.1"
                     /translation="MKKGTIILLVVLGVILLIGISFVSSYNSLVSLNESVDAQWGQVE
                     NQLQARYDKIPNLVNTVKGYASHEQEVFESIANARARLAGAGTVEEQVAASNQLESAL
                     ARLLVVVENYPNLKADAQFNQLMYELSGTENRIAVERMRYNEKVQEYNSRVKRFPTVL
                     IANMFGFGPRTYFEAAAGAEVAPRVEF"
     gene            complement(1595..2074)
                     /locus_tag="PLW82_02130"
     CDS             complement(1595..2074)
                     /locus_tag="PLW82_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOJ46104.1"
                     /translation="MLVDAFVEIPRGSQNKYEFDVKKGHFVLDRVLYGSVHYPGEYGF
                     IPNTIAEDGDPLDILVLSNVPTFPGCMVKARVLGALEMVDDKGRDVKILAALDNDPRY
                     DELQTLKDVPHHILKEIEHFFQIYKQLEGKETAVDGWRDLDFALKVIEESRNNFAAL"
     gene            complement(2156..2231)
                     /locus_tag="PLW82_02135"
     tRNA            complement(2156..2231)
                     /locus_tag="PLW82_02135"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2195..2197),aa:Glu,seq:ctc)
     gene            complement(2235..2308)
                     /locus_tag="PLW82_02140"
     tRNA            complement(2235..2308)
                     /locus_tag="PLW82_02140"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2274..2276),aa:Gln,seq:ctg)
     gene            complement(2667..3407)
                     /gene="mtnP"
                     /locus_tag="PLW82_02145"
     CDS             complement(2667..3407)
                     /gene="mtnP"
                     /locus_tag="PLW82_02145"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOJ46105.1"
                     /translation="MKVGIIGGSGVYDAELFQTTADKITTPYGEVEFERGVFGGEEIF
                     FLPRHGKGHSVPPHLINYRANIAAFKMLGVERILATSAVGSLRAELVPGTFVILNQFL
                     DFTKRRALLFDETQVKHLDFTEPYCRQINAILKEELQSLGIKNAEGCYVCTEGPRYET
                     PAEVKMFAQLGGDVVGMTNVPEVTLAKEAEICYSCICTVTNLGAGISEEPLEHEKVVE
                     VMKANSKNLKKLLLKAIPRLQQEPKTCK"
     gene            complement(3404..4384)
                     /locus_tag="PLW82_02150"
     CDS             complement(3404..4384)
                     /locus_tag="PLW82_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOJ46106.1"
                     /translation="MKELLKKLTQACGPSGSEQCIREIIKSELPNYETRTDALGNLIV
                     EKKGPKSKIMFSAHMDEIGIIVTHIDKEGFLRFDFIGGVNPFYLPGERVQFQNGTLGV
                     IGVEKLDKIADLQKSNLYIDIGVESKEEAMKLVKIGDTACFTSPFIDLGNRLVSKAMD
                     NRVACALLIKLMQELPETDNHIVAVFTVQEEVGLRGAKTAAYGIDPDYGLAIDVTATG
                     DTPKAMTMDVSLGKGAAIKVKDNSLLAHPYVKDKLVELAEANNIPYQMEVLEFGGTDA
                     GAIHLNREGVPSGVISVPTRYVHSRSEMVDVRDLDAALALAKAFAKADLK"
     gene            complement(4381..5406)
                     /locus_tag="PLW82_02155"
     CDS             complement(4381..5406)
                     /locus_tag="PLW82_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOJ46107.1"
                     /translation="MFLKELSEARGVSSREHEVRDLIREYITPHVDEVFNDSLGNLYG
                     VKGKDKPGPKVMLAAHMDEVGLMITTIEKNGLLRFQPVGGIDPRVLVSKPVYIGKDKV
                     VGVIGAKAIHLQRPEERTQVLGFDDLYIDIGAVDDDSAKKLVKPGDVATFATSFGEMG
                     DLVVGKAFDDRAGCTALVELTKLEFNCPVYFVFTVQEEVGLRGAGPAAYRVGPDVCLV
                     LEGTTASDVAGTDEEGYSTTVGAGPAVSVMDRSVIAHPKVVGRLFRLAEEHGVPIQNK
                     RTITGGTDAGAISLSREGVPTAPIAVPCRYIHSPVTVMSKSDLEHMIKLVELFVHDIA
                     DKGVDFE"
BASE COUNT         1282 a         1462 c         1205 g         1472 t
ORIGIN      
        1 taatggctcc cttattttag gaaacctatt ttacaccatt atacggactc aactttgact
       61 agaaacaact ttgggaattt actgtaattc attgtggtga taagggcaaa aaaaactagc
      121 ctatgtttgt gcataggcta acaaattacc actttcctga tgcccccccg ccaccgctgc
      181 ggcctccacc tccatagcgg ttccttccag aagagcctct gtaactgcct cctctgtagc
      241 taccaccacg ataggagccg cctctgagca gcgaatggag tattgcccct acgatcatac
      301 cgccggtaaa ccggaaatcc agtaaaacta gggcaacaat gatcacaaac aagatcaacc
      361 cggctagagg gttcggaccg tcattagttt catatctgga aatgccttct gtctccccag
      421 ccacgcgagg aactgcatcg ccttgtgcga tgatgcgctc ttctagaatt gcaacgaact
      481 caatcagtcc gttgatgtcg ttattcgcca gcgccgggcc tacgtacttc tgcaggttta
      541 cacctacgaa gctgtcgggc agagcaccct ccaaaccata acctacttcg atgcgcatct
      601 cgcggggatt tagggagatt agaagaagga gtcctttatc ttcacccttc ttgccaattc
      661 cccaatcctc gaacagctta actgcataca tctctatcga ttcatttccc agatcgggaa
      721 cggttacaac cgccatttcc acaccagtac gctggtacat agcttctata cggctgtcca
      781 gaaggtgttc gttactcaga ataccagcaa aatcatgaac atttgtaccg ggaatcgggg
      841 aaagagcaca gactgccaaa ggcagcatca gcaggactaa gatgatgtaa agtcttttca
      901 taggttgaga aagtagcctt cccttctccc tccatcctta atatcgctat ctaaaattcc
      961 actctcggtg ccacttcggc gccagctgct gcttcgaaat acgttctcgg accaaagccg
     1021 aacatatttg ctatcagtac agttggaaaa cgctttacgc ggctattgta ctcctgcact
     1081 ttttcattat agcgcattct ctctacagct atacggttct ccgttccgct cagttcgtac
     1141 atgagttggt tgaactgggc atcagccttg aggttaggat agttctctac taccactaga
     1201 agtcttgcca aagccgattc tagctgattt gatgctgcaa cctgttcttc aacagttcca
     1261 gctccggcca gtcttgcgcg agcattggca atggattcga aaacctcctg ctcatggctg
     1321 gcataaccct ttacggtgtt taccaggttg ggaatcttat catatcttgc ttgaagttga
     1381 ttttccacct gtccccactg agcatcaaca gactcgttta atgaaaccaa ggagttgtag
     1441 ctgctcacaa acgaaatacc gataagcaaa ataacaccga gtactacaag gagaattatt
     1501 gtaccttttt tcatgatgtt ggcaaaatcg ccctttcctc ccatctgtta ttgatgtttc
     1561 cacctgcttc tactatatgt ttctaggttt tgtttcacaa tgctgcgaag ttatttctcg
     1621 actcttcaat aactttcaga gcaaagtcca gatcccgcca gccatcgacg gcagtctctt
     1681 tcccttcgag ctgtttgtag atctggaaga agtgctcgat ttccttcaat atatggtgcg
     1741 gtacgtcttt tagcgtctgt agttcatcgt agcgggggtc gttgtccaaa gccgcgagaa
     1801 ttttgacatc tctcccttta tcatctacca tttccaaagc cccaagcacc ctggccttga
     1861 ccatgcatcc cggaaacgtt ggtacgtttg atagaactag aatgtctaaa ggatctccat
     1921 cttcagcgat tgtattggga atgaaaccgt actccccggg atagtgaacg gaaccgtaaa
     1981 ggaccctgtc caaaacaaaa tgacctttct tcacatcgaa ctcgtacttg ttctggcttc
     2041 ctctgggaat ctcaacaaaa gcatcgacta acacatctct gcccccttcg gagaaaatcc
     2101 tttagaattt gccaaaaagc ctgcaaactt tcagtttgca ggcttttcat tgctatggta
     2161 gccccaaggg gatttgaacc cctgtcaccg ccgtgagagg gcggcgtcct aggccactag
     2221 acgatggggc catttggctg gaggagaagg attcgaaccc tcgccggcgg ggccagaacc
     2281 cgctgtccta ccactagacg atcctccaat tacctcagcg gcaactctta ttctacttca
     2341 tagcactgaa accgtcaagt ccttttagag aacttttctg ctttttagcc tgcctcttag
     2401 ccatttttta tgaaaaacct acaacgccta gtttgtcccc acaaacagct atgttatcca
     2461 aaagcccaca gtgctactcc atgagttttt gaatccattg ttatgtacgg gaaaaggttc
     2521 tttcggggag atgccctgcc tgtgcctaat aactctgtaa agggcagatc ccaggccagc
     2581 ttttatagat ctgccctcta ttaacctaaa ttcctttgcg tagatcaggt ttgccctgct
     2641 tagaagtggg tcttggttga atccttttac ttgcaggttt tcggttcctg ctgcaggcgg
     2701 ggaattgcct tcagcagaag ttttttgagg tttttggagt ttgctttcat cacctcaacc
     2761 accttctcgt gctcaagggg ctcttcagag attcccgctc cgagatttgt cacggtacag
     2821 atgcagctgt agcagatctc cgcttccttg gccagcgtga cctcaggcac attggtcatg
     2881 cccacaacgt ctccgcctag ttgggcgaac atcttaacct ccgccggggt ttcgtaacgc
     2941 ggcccttctg tgcagacata gcaaccttcg gcgtttttga taccgagact ctgcagttct
     3001 tctttgagta tagcgttaat ctgcctgcag taaggttcgg tgaaatccaa atgcttcacc
     3061 tgagtttcat cgaagagaag ggctcggcgt ttggtaaagt ccagaaactg attgaggatg
     3121 acaaacgttc ccgggaccag ttccgctcgt aaagatccca ccgcagaagt ggccaggatc
     3181 ctctctacac cgagcatttt gaaagctgcg atgtttgccc tgtagttgat caagtggggc
     3241 gggacgctgt ggcctttgcc gtggcgggga agaaagaaga tctcttcccc gccaaaaact
     3301 cctctttcaa attcaacttc gccgtaaggc gtagttatct tatctgcggt agtctgaaag
     3361 agttcggcat cgtagactcc tgagcctccg atgatgccta ctttcatttg agatcagcct
     3421 tggcaaaggc tttggcaaga gctaaagctg catcaaggtc tctgacatcg accatttccg
     3481 acctgctgtg aacgtagcgg gtgggaacgg aaatcacccc tgacggaacg ccttcacggt
     3541 tgaggtgaat cgctccggca tcggttcctc cgaactcgag gacctccatt tgatagggga
     3601 tgttgttggc ttctgcaagc tcaaccaact tgtctttcac gtatggatga gccagaagcg
     3661 agttgtcctt caccttgata gcggcgcctt tccccaagga cacgtccatg gtcatggctt
     3721 ttggagtatc tccagttgca gtgacatcaa ttgccaggcc gtaatctggg tcgataccgt
     3781 atgccgctgt cttggcacct ctcaaaccca cttcttcctg gactgtgaac actgcaacaa
     3841 tgtggttgtc ggtctctggt agctcctgca tgagcttgat caatagcgca caagcaactc
     3901 tgttgtccat ggctttggag accagtctgt tgcccagatc gataaaaggc gaagtgaagc
     3961 aggcggtgtc gccgattttc accagcttca tggcttcttc tttactctcc accccgatat
     4021 cgatgtagag gtttgatttc tgcaaatcgg cgattttatc cagtttttcc acgccgataa
     4081 cgcctaaagt cccgttttgg aactgcactc tttctcctgg gagatagaag gggtttactc
     4141 cgccgataaa gtcaaagcgc aaaaaccctt ctttatcgat atgggttacg attatcccga
     4201 tttcgtccat atgcgcagaa aacataatct tgctcttagg tccctttttc tcgacaatca
     4261 agttgcccag cgcatcggtt ctggtttcgt agtttggcag ctcggatttg ataatctcgc
     4321 ggatacactg ctcggaacct gaaggaccgc aagcttgcgt tagtttctta agcagctctt
     4381 tcattcaaag tcaacccctt tatctgcaat atcatgcaca aacagctcta cgagcttgat
     4441 catatgctcc aagtcggact tgctcatcac ggtaaccgga gagtggatat atctgcaggg
     4501 aacggcaatt ggagcggtgg gcacgccttc ccggctgagg gagatcgcgc ctgcgtctgt
     4561 gccgcctgtg atagtccgct tgttttggat cggtacaccg tgttcttcag caagcctaaa
     4621 cagtctgccc acaactttgg gatgagcgat aacgctgcgg tccataaccg aaactgcagg
     4681 accagcaccg actgttgtgg aatagccttc ttcatctgtg ccggctacat cagacgcggt
     4741 ggttccctcc agaaccaaac agacatcggg acccactctg taagctgcag ggcctgctcc
     4801 tctgagaccg acttcttcct gcacggtaaa gacgaagtaa accgggcagt tgaactccag
     4861 ctttgtcagc tcaaccaatg cagtacagcc ggctctatcg tcgaaggctt tacctaccac
     4921 caagtccccc atctctccga aagaagtagc aaaagtcgcc acatcgccgg gtttcacaag
     4981 cttcttggcg ctgtcatcgt ctactgcgcc gatgtcaata tagagatcgt caaaacccag
     5041 gacctgggtt ctttcttcag gtctctgcag gtgaatggct tttgcgccaa taacaccaac
     5101 taccttatct ttgccgatgt aaacgggctt ggaaaccaaa acccggggat ctataccgcc
     5161 caccggttga aaacgcagaa gtccgttttt ttcaatagtg gtgatcatca gccccacttc
     5221 gtccatatgg gctgcgagca tgactttggg tcctggttta tcctttccct taactccgta
     5281 caagttcccc agagagtcgt tgaagacttc atccacatga ggcgtgatgt actctctgat
     5341 caggtctctc acttcgtgct ctctgctcga gacgccccgt gcctcactta actcttttaa
     5401 aaacatggca atccctccac a
//
LOCUS       DAOSDA010000056         5397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9593_length_5397_cov_3.502621, whole genome shotgun sequence.
ACCESSION   DAOSDA010000056 DAOSDA010000000
VERSION     DAOSDA010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9593_length_5397_cov_3.502621"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLW82_02160"
     CDS             <1..310
                     /locus_tag="PLW82_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46108.1"
                     /translation="GGLIDVFDLEDINKEALAKGLRPAEFKPVILGITKASLATESFL
                     SAASFQETTRVLTDAAIKGKVDTLIGLKENVIIGKLIPAGTGMERCRRIEIMPEEESI
                     "
     gene            428..679
                     /locus_tag="PLW82_02165"
     CDS             428..679
                     /locus_tag="PLW82_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOJ46109.1"
                     /translation="MPLEQLKTASKKAVGTKQVLRAIEKNAASLVFVAQDADADIIGK
                     INTSCQMHGVEIKPVKSKTQLGQVCGIDVAAATAAILKD"
     gene            703..1122
                     /gene="rpsL"
                     /locus_tag="PLW82_02170"
     CDS             703..1122
                     /gene="rpsL"
                     /locus_tag="PLW82_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003559489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOJ46110.1"
                     /translation="MPTISQLIRKGRKSKSKKSASPALLFVQNTLKGREIEIPKGAPQ
                     KRGVCTKVSTMTPKKPNSALRKVARVRLTNGIEVSSYIPGEGHNLQEHSVVLIRGGRV
                     KDLPGVRYHIIRGAMDTAGVQDRRQARSKYGTKRPKE"
     gene            1162..1632
                     /gene="rpsG"
                     /locus_tag="PLW82_02175"
     CDS             1162..1632
                     /gene="rpsG"
                     /locus_tag="PLW82_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOJ46111.1"
                     /translation="MPRRGHVPKRSVLPDPIYKSTVLSQFINKLMLDGKKGVAESIMY
                     GALDIVAAKSGQDPLEVLNKALENVMPVVEVKPRRVGGATYQVPVEVPANRRMALAMR
                     WMIGIARTRGEKTMAERLAAEILEAADNTGGSVRKKEDTHRMAEANRAFAHYRW"
     gene            1859..3925
                     /gene="fusA"
                     /locus_tag="PLW82_02180"
     CDS             1859..3925
                     /gene="fusA"
                     /locus_tag="PLW82_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ46112.1"
                     /translation="MPREFPLEKTRNIGIMAHIDAGKTTTTERILFYTGRVHKIGETH
                     EGAATMDWMVQEQERGITITSAATTCNWLGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VAVFCAVGGVEPQSETVWRQADRYHVPRIAFINKMDRTGADFFRVVKMMHDRLAANAV
                     PIQIPIGSEDSFVGMVDLITNQAIIYEDDLGTKLDAEPIPEEMADLVAEKRQELIEAI
                     VETNEELMMRYLDGEEFTAEELKAALRAACCRNEIVPVLCGSAFKNKGVQKLLDAIID
                     YLPSPIDVPDIVGKNEAGEEVTIRSSEDEPFAALAFKIMSDPFIGKLCYFRVYSGSLE
                     AGSYVYNSTRGRKERVSRLVYMHANHRQEVAKVHAGDIVAGVGLKNTTTGDTLCDENR
                     VVILESMTFPDPVIDIAIEPKTKNDQDKLSQALQRLAEEDPTFHVSTNEETGQTIIAG
                     MGELHLEIIVDRLLREFRVGANVGTPQVAYKETITKAARAEGKFIRQSGGRGQYGHVV
                     LELRPNEHGKGFVFENKIVGGVVPKEYIGPVEEGIKEAMLSGVLAGFPVVDVIVSLVD
                     GSYHEVDSSEMAFKIAGSMGFKEGVAKANPVLLEPVMKVDVTTPDDFLGDVIGDLNSR
                     RGRIEGMESRPGGQEVHSYVPLAEMFGYVNQLRSLTQGRATYVMQFSHFEPVPASIAE
                     TIINKG"
     gene            3953..5146
                     /gene="tuf"
                     /locus_tag="PLW82_02185"
     CDS             3953..5146
                     /gene="tuf"
                     /locus_tag="PLW82_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOJ46113.1"
                     /translation="MGKAHFERTKPHVNIGTIGHVDHGKTTLTAAITKYLGQKGYAQV
                     RKYDEIDNAPEEKERGITINTSHVEYETDNRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKADMVDDPELIELVEMEVRDLLNQ
                     YEFPGDDTPFVVGSALKALESDDPNDQWSQAIGKLMDIVDEYIPQPERAIDKPFLMPV
                     EDVFTITGRGTVATGRVERGTIKVGEQVEIVGLRETRATVVTGVEMFRKILDVAEAGD
                     NVGILLRGIERKDVERGQVLAKPGSITPHTKFEAQVYVLKKDEGGRHKPFFSGYRPQF
                     YFRTTDVTGTITLPEGVEMVMPGDNITMTVELLAPIAMEENLRLAIREGGHTVGAGVV
                     SKILK"
     gene            5237..>5397
                     /gene="rpsJ"
                     /locus_tag="PLW82_02190"
     CDS             5237..>5397
                     /gene="rpsJ"
                     /locus_tag="PLW82_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOJ46114.1"
                     /translation="MKQKIRIRLKAFDHVVLDQSARKIVETAETTGAKVSGPIPLPTE
                     KNIYTVLRSV"
BASE COUNT         1522 a         1237 c         1372 g         1266 t
ORIGIN      
        1 tggaggtttg atcgatgtct tcgatctaga ggacatcaac aaagaggcac tggccaaagg
       61 actcagacct gcagagttca aacctgtaat cctcggtatt accaaagcct cgctggcaac
      121 agagagcttc ctgtcggctg cttccttcca ggagaccacc agagtactca ccgatgctgc
      181 tatcaaaggc aaagtggata ctctcatcgg tctcaaggag aacgtcatta tcggtaagct
      241 cattccggct ggtaccggta tggaacgctg ccgtcgcata gaaataatgc ccgaagaaga
      301 aagcatttaa ctactttgac taacttttga ctctagaggg tgtcaatgaa attagcaatt
      361 gacaccccta gggtcaagat gctatacttc aatagtacgc cataaattgg ggaaggaggg
      421 ccttggcatg ccgttggaac aactaaagac ggctagcaag aaggcggtag gtactaagca
      481 agtgcttagg gctatcgaga aaaacgccgc tagtctggtg ttcgttgccc aggatgccga
      541 tgccgacatt ataggcaaga tcaacacgtc gtgtcagatg catggtgtcg agatcaagcc
      601 tgtaaagagc aaaacccaac taggccaagt atgtggaatc gatgtagcag ctgcaacagc
      661 agctatactt aaagactgag ttcactggaa ggaggtggca agatgccaac aatcagtcag
      721 ttaattcgca aaggtcgcaa gtctaagagc aagaaaagcg cttcaccagc tctccttttt
      781 gtgcaaaaca ccctaaaggg aagagagatt gagattccta aaggtgctcc gcagaagagg
      841 ggcgtctgca caaaggtttc cacgatgact cctaaaaagc caaactcggc tttgagaaag
      901 gtagctcgtg tacgtctcac caacggtatt gaagtaagct cttatatccc tggcgaaggc
      961 cacaacctgc aggaacactc tgtagtactt atcagaggtg gacgtgtaaa ggaccttccc
     1021 ggagtccgtt atcatataat tagaggcgca atggacactg ctggcgttca agatcgccgt
     1081 caggcccgtt cgaagtacgg aacaaagaga ccaaaagaat aataaagacg ctatcttcaa
     1141 tcgcaagggg gtgtagtgaa gttgccaaga cgtggacacg tacccaagag gagcgtcctg
     1201 ccagatccaa tttacaaaag cactgtttta agtcagttca ttaataaatt gatgctcgat
     1261 ggtaagaaag gcgtagccga atcgattatg tatggtgcct tagacatcgt tgcagcaaag
     1321 tctggtcagg atcctctcga ggtattgaat aaagctcttg aaaacgtaat gccggttgtt
     1381 gaggtaaaac ctcgtcgtgt aggcggtgcc acctatcagg ttcctgttga ggttcctgcc
     1441 aatcgtcgca tggctttagc tatgagatgg atgattggta tcgctagaac gcgcggagaa
     1501 aagactatgg cagagagact ggctgctgag attctcgaag cggcagataa caccggtggt
     1561 tctgttagaa aaaaagaaga cactcatcgc atggccgaag ccaacagagc gtttgctcac
     1621 taccgttggt agccatctac tgactaattg gtttttgcag accgaaacct gcacttacca
     1681 ctggcggtct cccgaccaaa tgcgtagcta ataactacgg attgtacgca tactagattt
     1741 gcttttctat gagctcggtc ggtctaggga cataagtcgc acccttattc agtcaaccgt
     1801 cagaagtaca agacttggcc tagctttggt gagactcagt agtaaggagg gaaagtgggt
     1861 gccaagagag ttcccactcg aaaaaacacg taatattggg attatggccc atattgatgc
     1921 cggaaagaca accacaacag aaagaatcct gttttataca ggcagagtcc ataaaattgg
     1981 ggaaacccat gagggtgccg ccactatgga ctggatggtg caggagcagg aaagaggaat
     2041 taccatcact tctgccgcta caacttgtaa ttggttgggt catcgcatca acattattga
     2101 tacacccgga cacgttgact ttacagttga ggtagaaagg tcgctccgcg tactcgacgg
     2161 agcagttgcc gtgttctgtg cggtaggagg cgttgaaccg cagtccgaaa ccgtgtggag
     2221 acaggcagat cgctatcacg ttcctcgtat agcgttcatt aacaaaatgg atagaaccgg
     2281 tgcagacttc ttcagagttg tgaagatgat gcacgatagg ctggcagcaa atgcagtgcc
     2341 aattcagata cccatcggca gcgaagattc gtttgtcggt atggtggatc tgatcaccaa
     2401 ccaggctatc atctatgaag atgacctggg aaccaaactc gacgccgagc ccattcctga
     2461 ggaaatggcc gaccttgttg cagaaaaacg ccaagaactg atcgaagcaa tcgttgaaac
     2521 caatgaagaa ctgatgatgc gctacctcga cggcgaagag tttaccgcag aggaacttaa
     2581 agctgctctt cgtgctgcct gctgcagaaa tgaaatcgta cctgtcttgt gcggctctgc
     2641 tttcaagaac aagggtgtac agaagctact cgatgcaatt atcgattatc tcccgtcgcc
     2701 aattgatgtt cctgacattg ttggcaaaaa cgaagctgga gaagaagtaa ccatcagatc
     2761 atccgaagac gaaccatttg cagcattggc cttcaagatc atgagcgatc cgtttatcgg
     2821 caagctctgc tacttcagag tgtactcagg ttctcttgag gccggttcct acgtatacaa
     2881 ctccacgaga ggacgcaaag agcgtgtcag ccgtttggtt tatatgcatg ctaaccacag
     2941 gcaagaagtt gccaaagttc atgctggcga cattgtggcc ggtgtgggtt tgaaaaatac
     3001 aaccacaggc gataccctct gtgacgaaaa cagagttgtc attctcgagt cgatgacctt
     3061 ccctgatccg gttattgaca ttgccatcga accaaagacc aaaaacgacc aggataaact
     3121 gtctcaagct cttcagcgtc tggctgaaga agatccaacc ttccacgtgt caactaacga
     3181 agaaaccggt cagacaatta tcgcaggtat gggcgaactg cacctggaga tcattgttga
     3241 tagactgtta cgcgagttta gagttggggc caatgtgggt actcctcaag tagcctacaa
     3301 agaaaccatc accaaggctg cgcgcgccga aggcaagttc attagacagt ctggtggacg
     3361 cggtcagtac ggtcatgtgg tactggaact cagaccaaac gaacatggca aaggttttgt
     3421 ctttgagaac aagatcgttg gtggcgtagt tcctaaggag tacattggac ctgtagagga
     3481 aggcatcaaa gaggccatgt tatcaggcgt tttagcagga ttccctgtag tagacgtcat
     3541 agtatcactg gtggatggct cgtaccacga agttgactca agtgagatgg catttaagat
     3601 agccggctct atgggcttca aagagggagt tgctaaagcg aaccctgtgc ttctcgaacc
     3661 tgtaatgaag gttgacgtta ccactcccga tgacttcctc ggcgatgtaa ttggtgatct
     3721 gaatagccgt agaggaagaa tcgaaggaat ggaatctaga ccgggaggac aggaagttca
     3781 cagctacgtg cctctggccg agatgttcgg ttatgtcaat cagctccgtt ctctgactca
     3841 gggacgtgct acttacgtaa tgcagttttc tcactttgag ccagttccgg ctagcatagc
     3901 tgagacaata ataaacaagg gttaacaccc acaaagcaag gaggctaaaa aaatgggtaa
     3961 agcacatttt gaacgtacaa aacctcacgt taacattggt accattggtc acgttgacca
     4021 tggtaagaca accttaactg ctgctattac caagtatctt ggtcaaaagg gttatgcaca
     4081 ggtaagaaag tacgacgaaa ttgacaacgc tccagaagag aaagaacgcg gtattactat
     4141 caatacttcg cacgttgagt acgaaaccga caatagacac tacgctcacg tagactgccc
     4201 gggtcacgct gactacgtta agaacatgat caccggtgca gctcagatgg acggtgccat
     4261 tctggttgtt tctgcagccg atggtccaat gccacagacc agagagcaca tcctgctcgc
     4321 tcgtcaggtt ggcgttccag ctatcgttgt cttcctcaac aaggctgaca tggtagacga
     4381 tcctgaactc atcgaactcg ttgagatgga agttcgcgat ctgctcaacc agtacgagtt
     4441 ccctggcgac gacacaccat tcgttgttgg ttcagctctc aaagcacttg aaagcgacga
     4501 cccgaacgat cagtggagcc aggctatcgg taagctcatg gacatcgtag acgaatacat
     4561 tccacagcca gagcgcgcta tcgacaagcc attccttatg cctgttgaag acgtattcac
     4621 aattacaggt cgcggtaccg ttgctaccgg tcgtgtagag cgcggaacaa tcaaagttgg
     4681 cgaacaggta gaaatcgttg gtctgagaga gaccagagct actgtcgtta cgggtgttga
     4741 aatgttccgt aagatccttg atgttgctga agctggtgac aacgtaggta tccttctccg
     4801 cggtatcgag cgtaaagacg ttgagcgcgg ccaggtattg gcaaagcccg gctccatcac
     4861 cccacacacc aagtttgaag ctcaagtcta cgttctgaag aaggatgaag gtggacgtca
     4921 caagccattc ttctccggtt accgtcctca gttctatttc agaacaactg acgttacagg
     4981 taccatcacc cttcctgaag gtgtagaaat ggttatgcct ggtgacaata ttactatgac
     5041 tgtagagcta cttgcaccta tcgctatgga ggaaaacctg cgtctcgcta ttcgcgaagg
     5101 cggacacacc gttggcgccg gtgttgtaag taagattctt aagtaatagt tccttcacga
     5161 ggtagcgact aagtcttacc tttgtcgcta ccgttttttt gggaccttga caatagtcat
     5221 aaaaggaggg aacacagtga aacagaagat aagaattcgc ctcaaggcgt ttgatcatgt
     5281 tgttttggat caatcagcac gcaagatcgt ggagacagca gagacaactg gtgccaaggt
     5341 ttctgggcct attcctctgc caactgagaa aaacatctat acagttcttc gctctgt
//
LOCUS       DAOSDA010000057         5351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9696_length_5351_cov_3.086858, whole genome shotgun sequence.
ACCESSION   DAOSDA010000057 DAOSDA010000000
VERSION     DAOSDA010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9696_length_5351_cov_3.086858"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLW82_02195"
     CDS             complement(<1..370)
                     /locus_tag="PLW82_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOJ46115.1"
                     /translation="MLSAKEQLELLERGTAQIISREELLQKLERSVATGKPLREKLSV
                     DPTRSDIHLGHTVPLQKLRQFQDLGHTAVLIIGDYTAMIGDPSGKSSTRPRLSYEEVL
                     ANAKTYQDQVFKILIPEQTEV"
     misc_binding    complement(416..621)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            953..3463
                     /locus_tag="PLW82_02200"
     CDS             953..3463
                     /locus_tag="PLW82_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOJ46116.1"
                     /translation="MSKKKQKQPKKKRKVLKIILVIMLIIIGIVGGFSATLIASVLKT
                     VPKLEDIKFSPRLASSIYDRNGELIQRLYTENRIWVSIDDIPQQMKDAIIAIEDKNFY
                     KHHGVDPIAITRSIYLNIKTRNLTAYGGSTITQQLAKNAFLTQEKKLIRKIKEAIWAI
                     QIERRYTKDEILETYLNEIWFGHGAYGVEAASYLYFGKSVKDLSLEETAMIAGVTNNP
                     GLWSPRYDMEAAKERRNTVLRRMWEEGYITEEVYRETIQKPIVLKVEEPNNKKVASYF
                     VDQVIQYLLKKFDVDTVYGGGLKVYTTLDLNMQRIAEETLLSSLPNGGVDKNGLAQPQ
                     GALITLDPRTGEVLAVVGGRGQDKLNRAYQTYRQPGSAMKPFIYATALAQGYTPATIM
                     VDEPVEYRVPSGDLWTVRNYHLDYKGPMTLRYALEMSINVVAVKLLDQIGIENTVKTV
                     QNLGITSLVTTGAVNDKALAPLALGALTKGVSLVQMAAAYGAFANQGIYCEPYFISRI
                     EDPYGKVLEENHVKPKAVLDEKVAYVLTEMLKGVVTDGTAKQANIGRPSAGKTGTTDN
                     YYDAWYVGYTPDLVTAIWVGEDFPSEMVYGGIRYGSWDTARIWGAYMKKITEGREVLD
                     FPRPDGIVEIEICTKSGLIAQNNCPEDTVRKEVFIRGTEPKTVCTLHDEEPAAKNRNL
                     ISLDMNWDVEVPESQPKEVPEEKSTNLLDTLLGIFTGGSKSSGKKDEEPADEPVTKQE
                     ETPAAPTTESSTATKINIPPVTNQVVVPQSPEPPKSTGEASQGTTSGEPDYLVTVEIC
                     ADSDKLATPACPASKVHRIKFFRGTEPKEECDVHGGIQ"
     gene            3602..5047
                     /gene="gltX"
                     /locus_tag="PLW82_02205"
     CDS             3602..5047
                     /gene="gltX"
                     /locus_tag="PLW82_02205"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOJ46117.1"
                     /translation="MCDVRVRFAPSPTGDPHVGNIRTALFNYLYARHTGGKFIIRIED
                     TDQVRLKEGSDQAIFDSLRWLGITWDEGPDIGGPYGPYRQSERLDLYKKHALELVEKG
                     MAYYCFCSAERLENMRKLQEAAKKAPMYDRKCLGLTKEEVEEKIAAGEPYVIRLKVPR
                     DGTTEFDDLIRGRVSFENSVIDDQVLIKSDGFPTYHMACVVDDHYMKISHVIRAEEWI
                     SSTPKHIILYEAFGWTPPKFAHAPMVLAPDRSKLSKRHGATGVKEFRELGYLPEALAN
                     FLAFLGWAPQDEREIFSLEELAKEFEIERVNKSGAIFDMNKLEWYNGYYIRHIETRDL
                     AQRVLPFLEKAGMQVKFDDYLVNCVALVQERMKTLAEAPELLDFFYELPEYDPELLIP
                     KKSDRETTKLALEKCYQVLKGLEDWSHDPMEEQLRQASTDLGIKSGQLLWPLRAAMTG
                     KSASPGAFEVTLVLGKEEALKRLENAISKLA"
     gene            complement(5059..>5351)
                     /locus_tag="PLW82_02210"
     CDS             complement(5059..>5351)
                     /locus_tag="PLW82_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46118.1"
                     /translation="ESLPELRHLCAQVGRDHLDHWLILRGEPPRLHEIADDPIGIQDL
                     AVGGKDLQKLFGESPQLGEVLERLAYLVREDPSLNTREKLLQLASDQKEPGK"
BASE COUNT         1446 a         1286 c         1404 g         1215 t
ORIGIN      
        1 ctacctcggt ctgttcggga atcaggattt taaacacctg atcctgatag gtcttggcgt
       61 tggccaggac ttcttcgtaa ctgagcctag gccttgtgct ggacttacct gaaggatcgc
      121 cgatcatggc agtgtagtcg ccgatgatca aaacggcggt gtggccgaga tcctgaaact
      181 gtctgagctt ttgcagaggc acggtgtgcc ccagatggat atctgacctg gtagggtcga
      241 cgctgagttt ctctcgaagc ggtttccctg tggccacaga tctctccagt ttttgcagga
      301 gttcttctct ggaaataatc tgggcagttc ctctttctaa gagttccaac tgttctttag
      361 cggataacaa cacgaatacc tccctcaacg cgtagtaaat aaaaaaaccg tcccccaaag
      421 ggacggcaga ttaccgtggt accaccctaa ttgccaaacg gccactttgt cctgataacg
      481 gcaggtttcc cggagctggc tacttacaag ttcaccagcc cagcccgagg atgtacttcg
      541 cctaaaccgg ctgctgattt tcaccaacca tcagctctct aaaagcccaa ttcagactac
      601 tcttcctgtc attgcttttg ttgtttttaa ttttagtgaa ataacttgtc taagtcaata
      661 ggcaggggtt ttggggatgc tatttttccg cctctttcag gcaaagctaa actcggaagg
      721 agatcgtcac ctccatttgc aaactggtcc taatagctat atactagttt agatggaaat
      781 aaaaggcggc agggttggcc tacaggtaac ctggacgccc aacaatactc ttttaagata
      841 ggaacatgtt caggagagtg tctttttgat aaacgaaacg agcaacaagg atttcgcagc
      901 ttcgtttgcg aacttattct gtagaatata gccgttgagg caggtgtata tgatgagcaa
      961 aaaaaagcag aagcagccga agaagaagag aaaagtccta aagataatat tagtcattat
     1021 gctgatcatt attggtatag ttgggggctt ttcggcaact ttgatcgcca gtgtcctcaa
     1081 aaccgtacct aaacttgagg atattaagtt cagcccgaga cttgcctctt ccatttacga
     1141 tcgcaacggc gaacttattc aaaggctcta cacggaaaac cgcatttggg tttcaattga
     1201 tgacataccc cagcagatga aagacgccat tattgctatt gaagacaaga acttttacaa
     1261 gcatcacggc gtcgatccaa tcgccatcac ccgctcgatc tatttgaaca tcaagacgcg
     1321 gaacctaacc gcttacggcg gcagtactat tacgcagcag ctggcaaaga acgctttcct
     1381 aactcaggag aaaaagctga tcagaaagat caaggaagcg atctgggcca ttcaaattga
     1441 acgcaggtac accaaggatg agatcctgga gacctacctc aacgaaattt ggtttgggca
     1501 cggagcatac ggcgtggaag cagcttcgta cctgtatttc ggaaagtccg taaaagacct
     1561 cagtcttgaa gaaaccgcta tgattgccgg ggtcaccaac aacccgggac tgtggtctcc
     1621 gcgatacgat atggaggctg ctaaagaacg cagaaatacg gtcctccgca ggatgtggga
     1681 ggaaggttat atcactgagg aggtctaccg cgaaaccatc cagaagccta tcgtccttaa
     1741 ggtagaggag ccgaacaaca agaaagtcgc ctcttacttc gtagatcagg ttattcagta
     1801 cctgctgaag aagttcgacg tagacacggt ttacggaggc ggtctgaaag tctataccac
     1861 cctagatctg aacatgcaga ggattgcaga ggagacgctc ctttccagtc tacctaacgg
     1921 gggagtagac aaaaacggct tggctcagcc tcaaggcgcg ctgattacac tcgatcccag
     1981 aaccggcgag gttttggcag tggtaggggg aagaggtcag gataagctca accgcgccta
     2041 ccagacttac agacagcccg gttctgccat gaagccgttt atttacgcca cagccctggc
     2101 ccaaggttac acccctgcga caatcatggt cgacgaacct gtggagtaca gagtaccctc
     2161 cggtgatctg tggacggtgc gcaactacca cctagactac aaaggcccta tgactttacg
     2221 ctatgctctg gagatgtcta tcaacgttgt tgcggtgaag ctgttggatc agatcggcat
     2281 cgaaaacaca gtcaagaccg ttcagaacct cggcatcacc agccttgtga ctaccggggc
     2341 cgttaacgat aaggcgctgg cacctctggc tcttggagct ctgactaaag gagtttcttt
     2401 ggtgcagatg gcagcagctt acggagcttt cgccaatcag ggtatctact gcgagcccta
     2461 cttcatcagc aggatcgaag atccttacgg gaaggttttg gaggaaaacc acgtcaaacc
     2521 taaagcggtc ctcgatgaaa aggtcgctta tgtgctgacg gagatgctga aaggcgtcgt
     2581 taccgatgga accgccaagc aagctaatat tggcaggcct tccgcaggca aaacaggaac
     2641 aaccgacaac tactacgatg cctggtatgt aggctacaca ccggacctgg taacagctat
     2701 ctgggtagga gaggacttcc ccagtgaaat ggtctacggc ggcatcaggt acggcagctg
     2761 ggatacagca cgcatttggg gcgcctatat gaagaagatt acagagggta gagaggttct
     2821 ggatttcccg agacctgacg gaattgtgga gattgagatc tgcaccaaat ccggactgat
     2881 tgcccaaaac aactgccctg aagatactgt gcgcaaagag gtgtttatca gggggacaga
     2941 gcccaaaacc gtatgcacct tgcacgacga ggagccggct gcgaaaaacc gcaatttaat
     3001 ctccttggat atgaactggg atgtagaggt tccggagtcc caacctaaag aggtgcctga
     3061 agaaaagagc acaaaccttc tggatacgct gcttggcatc tttacaggag gatccaagtc
     3121 gtctgggaaa aaagacgagg agcctgcaga tgaaccggtg acaaaacagg aagaaacgcc
     3181 tgcagcaccg accactgaat cctccactgc cactaagatc aacattccgc cagtgacaaa
     3241 tcaggttgtg gttcctcaaa gccccgagcc tccaaaaagc actggggaag caagtcaagg
     3301 tacaaccagc ggagagccag attacctggt aacggtggag atctgtgcag actcagacaa
     3361 actggcaact cctgcctgtc cggcgtctaa agttcatcgc attaagttct tcagaggtac
     3421 agaacctaaa gaagagtgcg atgtccacgg aggtattcag tagacagatt agcttggagt
     3481 ttttgcagca gttgtgtaaa atagataaag agcaaaggcc gatttggcct ttgcttctcc
     3541 atgttaacag actcgtaaaa gcttacagat atggaagtgg aatttggaag gagacaaaac
     3601 tatgtgtgac gtcagagttc gatttgcacc ctcgccaaca ggcgatcctc acgtgggcaa
     3661 catcagaaca gccctgttca actacctgta cgccaggcat acaggaggca agttcatcat
     3721 ccgcatcgaa gacaccgacc aggtaaggct caaagaaggc agcgatcaag cgattttcga
     3781 tagtttgcgt tggctgggca taacctggga cgaaggaccg gatattggcg gtccttacgg
     3841 tccgtatcgt caatcggaaa ggttggatct ctacaaaaag cacgcactag aactggtgga
     3901 aaaaggcatg gcctactact gcttctgctc agctgaaagg ctggagaaca tgcgcaagct
     3961 gcaggaagca gccaaaaaag caccgatgta tgatcgcaag tgtttggggc ttaccaaaga
     4021 agaggttgaa gagaagattg cagctggtga gccttatgta atcaggttga aagttccccg
     4081 cgacggaact acagagtttg acgatctgat tagaggcaga gtctccttcg aaaacagcgt
     4141 catcgacgat caggtgctga ttaaaagcga cggttttccg acctaccaca tggcctgcgt
     4201 tgtggacgac cactatatga agatctccca cgttatccgt gctgaagagt ggatctccag
     4261 cacgccgaag cacatcatcc tgtacgaggc attcggctgg actccaccga agttcgcaca
     4321 cgctcctatg gtgcttgcgc cagatcgcag caagctgagt aaaaggcacg gcgcaacggg
     4381 agttaaggag ttcagagagt tgggctacct tcccgaagca ttggcaaact tcttggcgtt
     4441 tttaggttgg gcgcctcaag atgaaaggga gatcttctcc ctggaggagc tcgctaaaga
     4501 gtttgagata gagcgcgtca acaagtccgg tgccattttt gatatgaaca aattggagtg
     4561 gtataacggc tattatatca ggcacattga aacgagggat ctagcacagc gcgtcctgcc
     4621 gtttttggaa aaagccggca tgcaggtaaa gttcgacgac tatctggtga actgcgttgc
     4681 gctggttcaa gaacgcatga aaactttggc agaggcacca gagctactgg acttcttcta
     4741 cgaacttccc gagtacgatc ccgaactgct catccccaaa aagagcgaca gggagactac
     4801 caagctcgct ttggagaagt gctaccaagt gttaaagggc cttgaagatt ggtcccatga
     4861 tccaatggaa gagcagctta gacaggcttc aaccgacctg ggcatcaagt ccggtcagct
     4921 gctttggcct cttagggcgg ctatgacagg caagtctgca tcaccaggcg cctttgaggt
     4981 aaccctggtt ttgggtaaag aagaggctct gaagcgcctg gaaaacgcta tttcgaaact
     5041 tgcttaagaa aaagagccct atttaccggg ctctttttgg tctgaagcca actgcaggag
     5101 cttttcgcgg gtgtttaaac tggggtcttc tctgacgagg tatgccagtc tttcgagtac
     5161 ttcgccgagc tgtggacttt ctccgaagag cttctgcagg tcctttccac ccactgcaag
     5221 atcttgaatg ccgatggggt cgtctgcaat ctcgtggagc cttgggggct cgcccctgag
     5281 aatcagccag tggtcgaggt ggtcacggcc tacctgggca cacaggtggc ggagttcggg
     5341 aaggctttcg g
//
LOCUS       DAOSDA010000058         5344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9715_length_5344_cov_2.721119, whole genome shotgun sequence.
ACCESSION   DAOSDA010000058 DAOSDA010000000
VERSION     DAOSDA010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9715_length_5344_cov_2.721119"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLW82_02215"
     CDS             <1..456
                     /locus_tag="PLW82_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46119.1"
                     /translation="GTLQADGTINHAVPTSSFGYVIPKYNQNQAKKDAAWEFLKWYTS
                     DETLAKMQKGFFEGPDRWVLAFGTRDSFQSAPFYEEHRELINEVVAAGRSMTSIPGGY
                     STYRYIDFAYNQVVLQGIDPKEAMEQAAKDSTAELERKRKEFARFLVDM"
     gene            571..1845
                     /locus_tag="PLW82_02220"
     CDS             571..1845
                     /locus_tag="PLW82_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46120.1"
                     /translation="MKKVLAVWVLILLVGVVCSAVKIEFWMVGWSNEMANVAKSIIDT
                     EFTPQTGIEVTIVPLAWGDYYQKSLLAIASRDTPDIFSLGSEIADFALRGGLIDLAAF
                     KPEEYAELEKGIFTSVMGPFSFKTARFALPIDVSGYIAVYRTDLLADLGMGIPETWDD
                     IRSWQPKVLANGMTLAYTNFEDMWGAYTHITQNGGQFFGSDGFSSALDREESVEGFVN
                     YIELFPVHQLPKVLVGPEPFIRGEQLSYADGLWVYGTLSQSAPTILGKWQVGLIPGTR
                     KDGKVNHGSFAGSTLLGISAFSNKMEEAWEFLKWFLSADVLQRIANGIMASSNGWIWL
                     PANKEAMSNVNLPVDVRDIYYRQIDESYPVPYAVNALVQYRFVTLAIQKCVTQGADPR
                     EEILKAAEDMNEEMARRKREYSRFLESLQIGG"
     gene            1944..2765
                     /locus_tag="PLW82_02225"
     CDS             1944..2765
                     /locus_tag="PLW82_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46121.1"
                     /translation="MKRSSYLLVLMLVWVVAAFSASAATLSARVVLPDGSGAPFATVT
                     IGDKVFTASETGKVEITYDGEKTALVKWLELEEEIAIGATMKFSNRLYYGAPYDANDF
                     VTWGPDIWEITESAMYCPGGLGNEAQIFLDDFYDNFVMRAKFSAEAESQWFYLRFMIR
                     GVEPGFNGYGVTVAMHPADQSSFARFNGSWDNYDVITEDIFIGPAMDPGVEDEIVVFA
                     KDEHIVVYLRNAGQKDYMKVVDHIDDDPGAFFDGGVGILNSFTVVEITEFSVFRY"
     gene            complement(2799..3284)
                     /locus_tag="PLW82_02230"
     CDS             complement(2799..3284)
                     /locus_tag="PLW82_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HOJ46122.1"
                     /translation="MFSDIKPRAFAHVRGGPLRPEITGTVWFLTAPEGGTYVAAEIKG
                     LPPYSPASPSRPPIGPFGFHIHEGSCCVVGDVNNPFLSAGPHYNPDGQPHGNHAGDLP
                     NLFGMDGTAVMIFFTNRFKPADVVGKTVIIHESPDDGTTQPSGNSGRRLACGEILEFR
                     N"
     gene            complement(3376..3972)
                     /locus_tag="PLW82_02235"
     CDS             complement(3376..3972)
                     /locus_tag="PLW82_02235"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOJ46123.1"
                     /translation="MIMRFSQIVYVALVYFVASIPFSFLVAKALTNTDIREFGDGNPG
                     AYNAWRYASKPAGYVGFTLDFLKGSLPIVIVKKLWPQSELMLFFTAVSSVSGHAWSWY
                     LHFHGGKCLAVSAGVLLTLLDKYMPLFWACVLGALRFLGVSPGGSVLITLCTANVHTY
                     FAVTSWYLRLTVLCISLIVLYKHKDVLFPNRSEAYMDV"
     gene            4175..>5344
                     /locus_tag="PLW82_02240"
     CDS             4175..>5344
                     /locus_tag="PLW82_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HOJ46124.1"
                     /translation="MRRVSLVFVVFVLLCTITYGSTSHPIVKGTFIQEWLTVHWTDEM
                     WDSELEYLKEVGIEFLIVAPTGFGIGDGNLRTIYPSKKPNWTDYYPGVDLLGNTLRAA
                     ERHGMQVVIGLNMDDRWWQIGVKDPQWLEGQVELGNQLAEEIYNLYYADYRDTICGWY
                     FVFEIDNLMVQQKNAVEVLAKAFNTHLDFLTELDPGLFFMWSPYMNSALATAESYGRF
                     WTELFREVRFREGDIFAPMDCVGGGGTTLENVARWFAEFRKAVDTKPGLLLYSNAENF
                     DHTDWTSATVGRFISQLQLVDEYVDGFINFAYPHYYSPNIIDHGFHKTYLTYVQTGEV
                     ESQPPTPPRSIQAVEMPDGSVFLIWEGGEDDVGIAGYLVYRDGRFVGKTQTPRKNA"
BASE COUNT         1329 a         1314 c         1470 g         1231 t
ORIGIN      
        1 ggtacactgc aggctgacgg tacgatcaac cacgccgtgc caacatcgtc atttggctac
       61 gtaattccta agtacaatca gaaccaggca aagaaggacg ctgcttggga gttccttaaa
      121 tggtatacct ccgacgagac cctagcgaaa atgcagaagg gcttcttcga aggaccggac
      181 cgctgggtac tggcgttcgg tactagagac agcttccaga gcgcaccgtt ctacgaagag
      241 caccgcgaac tcattaacga ggtcgttgca gcaggacgaa gcatgacctc tattcccggc
      301 ggctattcca cctaccgcta tatcgacttt gcctacaacc aggtggttct gcagggtatt
      361 gatcctaagg aagcaatgga gcaggctgca aaagactcca ccgctgaact ggaaagaaag
      421 aggaaggagt ttgcccgctt cctagtagat atgtaacagt aagcaaggct gcgtaacact
      481 tggttatgcg gccttgcttt cattgctctt tgcagcggta cataacccgt ctccaaccag
      541 gagacctaat acagtaaagg gggttttatc atgaaaaaag ttttagcagt ttgggtgtta
      601 atactgcttg tcggtgtcgt ctgctcagct gtaaagatcg agttttggat ggttggctgg
      661 agcaacgaaa tggccaatgt ggccaagtcc attatcgaca ccgagttcac gccacagacg
      721 gggatcgaag taaccatcgt gccccttgct tggggcgact actatcagaa gagccttttg
      781 gctatagcaa gccgcgacac tcccgacatc ttcagtttgg ggtcggagat cgcggacttc
      841 gcactgaggg gcggcctcat cgaccttgca gcttttaagc ctgaggagta cgccgaactg
      901 gaaaaaggga ttttcacctc ggttatggga ccattctcgt ttaagacagc acgctttgca
      961 cttcctattg acgtcagcgg ctacattgca gtctacagaa cggaccttct ggcagatctg
     1021 ggaatgggca tccctgagac ctgggatgac ataagaagtt ggcagccgaa ggtcctggca
     1081 aacggcatga cccttgccta caccaacttt gaagatatgt ggggtgccta tacccacatc
     1141 acccagaacg gaggccagtt cttcggttcc gacggtttct ccagcgcttt ggatcgggaa
     1201 gagtcggttg aagggttcgt caattacatc gagctgttcc cggtccacca gcttcccaaa
     1261 gtccttgttg gtccggagcc gtttatcagg ggagagcagc tgagctatgc cgacggcttg
     1321 tgggtttacg gtacgctgag ccagtctgct ccgaccattc tgggtaagtg gcaggtaggg
     1381 ctgatccctg gaacccgcaa agacggtaag gtgaaccacg gctcctttgc agggagcacc
     1441 cttcttggca tctcggcgtt ttccaacaag atggaagaag cttgggagtt tctcaagtgg
     1501 ttcctctcgg cagacgttct ccagcgcatc gccaacggca tcatggcctc atccaacggg
     1561 tggatctggc ttccggcaaa caaagaggct atgtctaacg tgaatctgcc ggtggatgtc
     1621 agagatatct actacaggca gatcgatgag agctatccgg ttccctacgc ggtaaatgcg
     1681 ttggttcagt accgcttcgt tacgctggcc attcaaaaat gcgtcaccca aggagccgat
     1741 cctcgggaag aaatcctgaa agccgcggag gacatgaacg aagagatggc cagacgcaag
     1801 agagagtaca gcaggttcct cgagtctcta cagatcggcg gttaacatct gtagaagaac
     1861 acccccagac agaaggaaaa cccagaaaaa cgcggaacta gtaatgtgac gcactattat
     1921 ataacagtca gaggggtgta gaaatgaaaa gaagcagtta cttgttggtt ttgatgttgg
     1981 tttgggtcgt tgcagcattt agcgccagtg cagctacact gtcggcacga gttgttcttc
     2041 ccgacggtag cggtgctcca tttgccacgg tgaccatcgg cgataaggtc tttaccgcat
     2101 cagagacagg taaggtggag atcacttacg atggcgagaa gaccgcttta gtcaagtggt
     2161 tagaacttga agaggagatc gccattggtg caactatgaa gttctccaac aggctctact
     2221 acggtgctcc atacgatgca aacgacttcg ttacttgggg tcctgacatc tgggagatca
     2281 ccgaatcggc aatgtactgc ccaggtggac tgggcaacga agcacagatt ttcctggacg
     2341 acttctacga caatttcgtg atgagagcga agttttctgc tgaagcagag agccaatggt
     2401 tctacctccg ctttatgatt agaggtgttg aaccaggctt caacggctac ggtgtaaccg
     2461 ttgccatgca tcctgccgac cagtcttcgt tcgcccgctt caacggtagc tgggacaact
     2521 acgatgtgat aaccgaggat atctttatcg gtcctgctat ggatccaggc gtagaggatg
     2581 agattgtagt gtttgcaaaa gacgagcaca ttgtggttta tctcagaaac gcagggcaga
     2641 aggactacat gaaggtagtg gaccacatcg acgatgatcc cggagccttc ttcgatggcg
     2701 gagtcggtat tctcaacagc ttcaccgttg tggagatcac cgagttttcc gtgttcagat
     2761 actaggatag agaaagggag agcgctattg ctctcccttt aatttctgaa ctccaaaatt
     2821 tcgccgcacg ctagccgacg tcctgagttg ccgctaggct gagtggttcc atcgtccggg
     2881 ctttcgtgaa tgatcaccgt tttgccgaca acatccgccg gtttaaacct gttggtgaaa
     2941 aagatcatga ctgccgttcc atccatgccg aagaggttgg gtaggtcccc agcatgattg
     3001 ccgtgaggct gaccatcggg attgtagtgg ggtcctgcgg acaagaaagg gttgttgacg
     3061 tcgcctacaa cgcagcagga tccttcatga atgtggaacc cgaaaggtcc gataggcggc
     3121 cgcgatggcg atgccgggct ataggggggc agacctttaa tctctgcggc aacgtaggtg
     3181 ccgccttcag gagcggtcag aaaccaaacg gttccggtga tctccggccg cagaggacca
     3241 cctctcacgt gggcaaacgc tctcggtttg atgtctgaaa acaactaaag catctccctc
     3301 gttttttaat gcgcagtaca cattatgcaa cggcaggaga aaaaggtata agagcttgag
     3361 aaaagcctcg tgagtctata cgtccatata cgcttcggag cggtttggaa acaggacgtc
     3421 tttgtgtttg tacagtacga ttagtgaaat gcacagtacc gtaaggcgca gataccaaga
     3481 ggtcactgca aagtacgtgt ggacgttagc agtgcagagg gtaatcagaa cgctgcctcc
     3541 gggagaaacg cctaaaaacc ggagtgcacc gaggacgcat gcccaaaaca gaggcatgta
     3601 tttgtctaga agcgtcagaa gcacaccagc agacaccgcc aggcactttc ccccatggaa
     3661 gtgtaaatac cacgaccatg cgtggccgct cacactggac accgctgtaa aaaacagcat
     3721 cagttccgac tgaggccaga gttttttaac gataactatg gggaggctcc ctttcaagaa
     3781 atccaaagtg aaaccgacgt atcccgccgg cttgctggcg taacgccaag cgttgtaggc
     3841 acccggatta ccgtcgccaa actccctgat gtccgtgttg gttagagctt tggcgacaag
     3901 aaagctgaag ggaatcgaag ccacaaagta gaccaaagcc acgtaaacga tctgggaaaa
     3961 acgcataatc atttcaccgg cctccatttt ctcttagagg tttcactacc tacattagtt
     4021 agcagtagga aaattccttg tccgattgtg ggtttttgta caaaggcatg ttcctctctt
     4081 agtgtggagc ttgaccggta tgttttacgg ctttcgaagg ccaaagcaga gcgaaaaatc
     4141 gatacagacc gcgttttccg aggaggggtt ttctttgcgc agagtttctt tagtttttgt
     4201 cgtgtttgtt ttgctgtgca ccattacata cggaagcact tctcacccca tcgtcaaggg
     4261 gacgtttatc caagagtggt taacggtcca ctggaccgat gagatgtggg atagcgaact
     4321 ggaatacctc aaggaagtgg gcatcgagtt tctgatcgtt gctcccacag gttttgggat
     4381 cggcgacggg aacctgcgga ccatctaccc tagcaagaag cccaactgga ccgactacta
     4441 cccaggagtg gatctcttgg gaaacaccct gagagcggcc gaaaggcacg gaatgcaggt
     4501 tgttattggt ctgaatatgg acgaccgttg gtggcagatt ggcgtcaagg atcctcagtg
     4561 gttggagggg caggtggagc ttggcaacca gcttgctgag gaaatctaca acctatacta
     4621 tgcagactat cgggatacga tctgtggttg gtacttcgtt tttgagatcg acaacctgat
     4681 ggtgcagcag aaaaatgcag tggaggtgct ggctaaagcc ttcaacaccc atctggattt
     4741 tctcaccgaa ctggatcccg gcctgttctt catgtggagt ccctacatga actcggcact
     4801 ggcaacggcg gaaagctacg gcagattctg gaccgagctc ttcagagaag tgcgcttcag
     4861 agagggagac atcttcgctc ctatggactg cgtaggagga ggggggacaa ccttggagaa
     4921 cgttgcccgc tggtttgcag agttccgcaa agcggtggac accaagccgg ggcttttgct
     4981 gtattccaat gccgaaaact tcgatcacac ggactggact tcggcaactg tggggcggtt
     5041 cattagccag ctgcagctgg tagatgagta cgttgacggt ttcattaact tcgcctatcc
     5101 ccactactac agcccgaaca tcatcgatca cggttttcac aagacctacc tgacctacgt
     5161 ccagaccggc gaagtggagt cccaaccgcc aactccgccg cgctccatcc aagctgtgga
     5221 gatgcctgac ggatctgtct ttctcatctg ggaaggtgga gaggacgacg ttggcatcgc
     5281 tggatatctg gtttacagag acggcaggtt cgtgggcaag acccaaactc ccaggaaaaa
     5341 cgca
//
LOCUS       DAOSDA010000059         5325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9751_length_5325_cov_3.187287, whole genome shotgun sequence.
ACCESSION   DAOSDA010000059 DAOSDA010000000
VERSION     DAOSDA010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9751_length_5325_cov_3.187287"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1088..2155)
                     /locus_tag="PLW82_02245"
     CDS             complement(1088..2155)
                     /locus_tag="PLW82_02245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ46125.1"
                     /translation="MRRNWQIKLLAIALLFSLLLVTGCTKQVFKFDIPESEIPTITVL
                     AYHRILDGGPSFIDVSTSMFDNHLTWLEQNGYNVISYDEFIDFLDGKGNLPEKPVLIT
                     FDDGYKETYTNAMPILAKHEMPAVLFIYSNRMKDYSFDGGLSVTELREMQRKGWSIQA
                     HTVTHADLSLFSPHAKASAMYEMAQNKKDIESKLGTEVTGLAYPYGAYTPEVEQWAQE
                     AGFKAAFLIEEGPNTYDDIHPYRIKRHMLYQKDTLEDFARKVTSSPLQVIRTVPEQAQ
                     VIDYRPATVIADVVLPEGVEVVKVYADIESEDLSARFDAEKQRVIVDTASVGRGHHNM
                     TIKLLGSDGRTYVYGWSYVIQ"
     gene            complement(2193..3134)
                     /locus_tag="PLW82_02250"
     CDS             complement(2193..3134)
                     /locus_tag="PLW82_02250"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ46126.1"
                     /translation="MFLYEKLVSDVSDFTVDVEKPADFDVFWEETIRLAKDKLEFTLE
                     QVPFPLKARVFKANLVGYGDTEIKGWYLLPENEDSTYPLVLTFHGYRGYASSPFYFAQ
                     YLLNGMAVLAFDLRGQVGQSGDEGYASGHVPGWMNQGLDSPYDCYYRRLLVDCMRMVD
                     MAKSLPDVDASRIAVKGASQGGGLALAVAALCDVALCVAGVPNMCYMEYGVLNSSSSL
                     KEVAEYLKLYPEKAPAYFTTLRYFDNVNLADRIKCPVLVSVGMQDPVCLPKTVYAAYN
                     KIMAPKQILAYPFSGHDGGGALFEQAAMEFILEHFNL"
     gene            complement(3254..3976)
                     /locus_tag="PLW82_02255"
     CDS             complement(3254..3976)
                     /locus_tag="PLW82_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46127.1"
                     /translation="MGAIRDRKVVAVDVRDQEVIFDVNLTEFNSGAWAKAVWVNGKGN
                     VFAPGSDVPEIYVFDSDLMWLETYYIDGFGITDCEGAVADDHGNIYASDRSGIGGIYK
                     IRHEDGDLDAKWGQGGWADVGHVRLPYLKGDAIYAVDHTTSILYKIDLATGKVTTLAN
                     LAPSGFAVAVDDNGVIYVAHYSNPSVAVSVIENGKVTEYSPSAFGLTSNVGGIAVTKD
                     GSTLYLVEEQSGIGGLVLGFTK"
     gene            complement(3989..4114)
                     /locus_tag="PLW82_02260"
     CDS             complement(3989..4114)
                     /locus_tag="PLW82_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46128.1"
                     /translation="MKRFAVVLLMFLIVALPVFAWKNVLEVHVGETARGVALSPD"
     gene            4371..5186
                     /gene="cdaA"
                     /locus_tag="PLW82_02265"
     CDS             4371..5186
                     /gene="cdaA"
                     /locus_tag="PLW82_02265"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOJ46129.1"
                     /translation="MAALLKNITLLDIIDILLVAFVVYRVMLLIKGTRAVSLLKGIAV
                     VFAVSVLSEFLGFRTVNWLIQQLVTLFLVALPIVFYPELRKVLERLGRGVLKEERFGL
                     FDRGQSQKAIGEIIRAVEKFSETATGALIVLKRDTGLLEYIETGTLIDSMVSAAALET
                     IFFPNTPLHDGAVIIEGDRIVAAGCLLPLSENPEISKELGTRHRAAVGLSEVSDAIVI
                     VVSEETGAISLAEDGKLNRYVTPKQLQERFLAQSSKASERFWPFGREGKQRES"
BASE COUNT         1360 a         1265 c         1325 g         1375 t
ORIGIN      
        1 ccgttccatc cgaagaagtt cgaaccctca ctggagcctg tagacaaaga cgccgtgctg
       61 gagaagtaac agtttcagtt aggacacgtt ccttggtttg tagtgatctt cctcatcgta
      121 aactgcgcgt tttgctgcag tctatacctt tacctgttcg aaaacgcggt tatataaaga
      181 agcaaggtag cgttgcaaaa ctacttgata agtagtagca acgctacctt actttattgc
      241 tggatactgt gcctttagtt taagtgccta tgaagaacct cgcgcaatcg gaagcataag
      301 aattgtgcat ttggaagttt gacaagaatg gaaactgctc aaaagaaaac ggtaagccaa
      361 ggccgtggct aactgttttt tcagcttgaa gtctcgttga gcaggggaag ttgcatcttc
      421 tcaagctttg tttgctctag cggtatcccc acagatgttg caccgcaggt gtcgtgccag
      481 atagtagcag accgaggaat gaatgctgcg gcttaggacg aagctttttg tgaacctgca
      541 tcgataggca gtcaaaaaac agggctaagt ctatctggcc tggtttaatt aaaacatgag
      601 accggctacc ttgttaatgt gagccacgcg tcctccgtta ccggagcggg atgggtaggg
      661 tgttttccca gagtcaacat taggagggtg tgagttgaaa tggaaaagtt cttgttcaca
      721 gcggtcattt ggctttgcgc gagcttgtat tgttaacggt tttgtacctt cgcgttaact
      781 ggattaacgt tggtccgtac cgcaaggaag caagtctgat ttacaaaacc caacatgaga
      841 ccttacaaag tatatctgag atgctgctga actcgaggtt gaaggctgtt gctccgtatg
      901 ttctcacctc tgtttcggta ctttataaca gaataatgga actacaaaag gcacgtttag
      961 attctatgta gccttgaaga aagcatgaga aaaccgctga gtaagtcagt gaccattaaa
     1021 acactgcgaa aaaacgccct ttacctattt tagtaggcaa agggcttgtt tgctttagat
     1081 gactgcgtta ttgaattaca tacgaccagc cgtagacgta ggttctgcca tcgcttccta
     1141 gaagcttaat ggtcatgttg tgatgaccgc gacctaccga ggcggtgtcg acaattaccc
     1201 gctgtttttc ggcatcaaat cgagcggaca ggtcttcaga ttcgatatct gcgtaaactt
     1261 ttaccacttc aacgccctct ggcaggacca catcagcaat gactgtagcg ggtctgtagt
     1321 caatgacctg agcttgctcg ggaacggtcc ggatcacctg caagggagaa ctggtcacct
     1381 ttctggcgaa gtcttcaagg gtgtccttct ggtagagcat gtggcgcttg attctgtaag
     1441 gatggatgtc gtcataggtg ttcggacctt cttcaatgag gaaggcagct ttgaatcctg
     1501 cttcctgggc ccactgttct acctcgggag tatatgcccc gtagggatag gccaatcccg
     1561 ttacctcagt tcccagtttg ctttcgatat cctttttgtt ctgggccatt tcatacatcg
     1621 ccgatgcttt agcgtgcggt gaaaacaggc tgaggtcagc atgggttacg gtgtgtgcct
     1681 gaatcgacca gcccttccgc tgcatctctc tgagttcggt aacagaaagg ccaccgtcga
     1741 aagagtagtc cttcatgcga ttggagtaga tgaaaagaac tgcgggcatt tcatgtttgg
     1801 ctaggatcgg catagcgttg gtgtaggtct ctttgtagcc gtcgtcaaag gtgatgagga
     1861 ccggtttttc aggaaggttg ccttttccgt ccagaaaatc gataaactcg tcgtaggaaa
     1921 taacgttgta accgttttgc tcaagccatg tcaggtggtt gtcaaacatg gacgtggaaa
     1981 catcgataaa tgaaggccca ccgtctaata ttctgtgata tgcaagaacc gtaatggttg
     2041 ggatctcgct ttcggggatg tcgaacttaa agacttgctt tgtgcaacct gtaacaagca
     2101 gcaagctgaa aagtagcgcg atggcaagca gcttaatctg ccaatttctt ctcatagttg
     2161 ttcactccta tattgttagt cttaggacaa acctacaggt taaagtgctc gagaataaac
     2221 tccatggctg cctgttcgaa caaagctccg cctccgtcgt gtccgctgaa cggatatgca
     2281 aggatttgtt tcggtgccat aatcttgttg taggcagcat aaacggtctt aggtaggcac
     2341 acaggatcct gcattcctac agacaccagc acagggcatt tgattctgtc tgccagattg
     2401 acgttatcaa aatatctcaa tgtggtgaag taagcaggtg ccttttccgg atacaacttc
     2461 aggtactcgg ctacttcttt gagcgaactg ctggagttta gcaccccgta ctccatgtag
     2521 cacatgttgg gaactcctgc aacgcacaag gccacatcgc acaaggcagc aactgccaga
     2581 gccaggccgc ctccttgaga tgcacctttc actgcgatgc ggctggcatc gacatctgga
     2641 aggcttttag ccatatcaac catccgcatg cagtccacaa gaagcctgcg gtagtagcag
     2701 tcgtacggac tgtctagccc ctgattcatc caaccgggaa cgtggccgct ggcataccct
     2761 tcatcgccac tttggccaac ctgtcccctc agatcgaaag ccagtacggc catgccgttt
     2821 aaaaggtatt gggcaaagta gaatggcgag gatgcgtaac ctctgtagcc gtgaaacgta
     2881 agcaccagcg ggtaggtgct gtcttcgttc tccgggagta gataccatcc tttgatctca
     2941 gtatcaccat acccaactag gttagctttg aacactctgg cctttagagg gaaaggaact
     3001 tgctctagag tgaactccag cttgtccttt gccaggcgaa ttgtctcctc ccaaaaaacg
     3061 tcgaaatccg ccggtttttc cacatctacc gtgaaatctg atacgtcgct tacaagtttt
     3121 tcatacagaa acatatgagg acctccctaa gataacccct tggggaacta cttacgtaaa
     3181 ttaggcagta gcaaaaaaaa gaccttcttt tatttacata aaagaaggtc tttttcagat
     3241 tttacattac agactacttg gtgaagccca gaaccaaacc gccgataccg gactgctctt
     3301 caacgaggta cagcgtggag ccgtctttgg ttacggcaat accgccaacg ttggaggtca
     3361 gaccgaaagc ggaaggtgag tattcggtaa ccttaccgtt ttcaataacc gatactgcaa
     3421 cgctggggtt ggagtagtgg gcaacgtaga tgacgccatt gtcgtccact gctactgcaa
     3481 atccacttgg tgccagatta gccaatgtgg taaccttgcc ggttgccaag tcaatcttgt
     3541 agaggatgga ggtggtgtgg tcaacagcat agatagcatc gccctttagg tatgggagac
     3601 gaacatgacc gacgtcggcc catccaccct gaccccactt ggcatccaaa tcgccatcct
     3661 catggcgaat cttgtagatg ccaccaatgc cactgcgatc ggaagcgtag atgttgccat
     3721 gatcgtcggc gacggcacct tcacagtcag tgatgccaaa accgtcgata taatatgttt
     3781 cgagccacat taaatctgag tcaaacacgt agatttccgg cacgtcgctg ccaggtgcaa
     3841 agacgttgcc tttgccattg acccatacag ctttagccca agcgccggag ttaaactcgg
     3901 tgagatttac atcgaaaata acctcttggt ctctaacgtc aactgcaacg acttttctgt
     3961 cgcggatcgc gcccacatag agcgtttttc agtctggact aagtgctaca ccccttgcag
     4021 tttcacctac atgaacttcc aaaacatttt tccaagcaaa aactggtaat gctacgatga
     4081 ggaacataag tagaacaact gcaaaccttt tcatgataaa acctcccttg taactaagtt
     4141 actgattaag cgttcgttat ggatttttca actcctgcta catgcaacgg gaaacttaac
     4201 acaaaaatct gattggggaa aatataggga ttatagggaa agactaagga ggcggcaact
     4261 tatcgtgcat gctagagata ttggacgatt ttaccgaaaa attctatctt tgccccatgc
     4321 tgcatggtaa gataaagaaa agaacaggca tcttcaggga gggtagacag atggcagctc
     4381 tgcttaaaaa catcactctg ctcgacatca tcgatattct tcttgtggcc ttcgtggtct
     4441 accgagttat gcttctcatc aagggcacaa gggcagtttc gctgctcaag gggattgccg
     4501 tggtctttgc tgtaagtgtc ctcagcgagt ttttgggatt tcgcactgta aactggctga
     4561 ttcaacagct agtaacgctg tttctggtcg ctctgcctat cgtcttctat cccgagctgc
     4621 gcaaggtttt ggaacggttg gggcggggag tattgaagga agagcgcttc ggcttgtttg
     4681 acaggggaca gtctcaaaaa gccattggcg agattatccg cgctgttgag aagttttccg
     4741 agaccgctac gggagctctg atcgtcctga aaagggacac aggacttctg gaatacatag
     4801 aaaccggaac cctaatcgat tcgatggtca gcgcagctgc cctggagacc atcttctttc
     4861 ctaacacccc tctacacgat ggggccgtga ttatcgaggg tgatagaatc gtggctgcag
     4921 ggtgcctcct gcccttatcg gaaaaccccg agatctccaa agaactcggt acccgccacc
     4981 gggcagcggt gggactgtcg gaggtcagcg atgccattgt gatcgtggtc tcggaggaga
     5041 ccggtgccat atctcttgcc gaagacggca agctcaaccg ctacgtcacg ccaaaacagc
     5101 tgcaggagcg cttccttgcc cagagcagca aggcgtcaga gcgcttctgg ccctttggac
     5161 gggagggaaa gcagcgtgaa agttaaaatc aaaagcagtg atgtctatct gcgggttctt
     5221 tcgctggtca ttgcggtgat cttctggctg tttgcaaagc aaaacgtgca gatccccaac
     5281 atctccgaaa ggcagcaata ccagaaggtt atgaagaaag tggtc
//
LOCUS       DAOSDA010000060         5313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9779_length_5313_cov_3.094903, whole genome shotgun sequence.
ACCESSION   DAOSDA010000060 DAOSDA010000000
VERSION     DAOSDA010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9779_length_5313_cov_3.094903"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLW82_02270"
     CDS             complement(<1..394)
                     /locus_tag="PLW82_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HOJ46130.1"
                     /translation="MTGAFVAVRTVILYFIILVAMRLMGKRQMVQLQPYELVTAIMIA
                     ELAVLPIGNVNMPLTHAIYPIALLGVLEFLISTISLKSEKVRTWIAGRPTIIIENGHI
                     RENVLRALRYNLDELMGQLRNSGYSDVND"
     gene            504..1532
                     /locus_tag="PLW82_02275"
     CDS             504..1532
                     /locus_tag="PLW82_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ46131.1"
                     /translation="MKLRVGVIGTGAIAKYRHLPEYAAREDVEIVALCDIIKEKADKL
                     AVRYGVESVYTDYKQMIENEELDAVSVCTPNYLHAEMSIFALNHGLHVLVEKPMATSL
                     DEANAMIEAAKKQERVLMVGQNQRLAPMHVLAKEIIESGMLGKVNTFRTVFGHAGPEH
                     WSKTGKWFFDKKQAFAGSLADLGVHKMDLIQFILGDVDEVACFIDTLEKKDCGVDDNA
                     VAVLRLKNGIMGTVETSWTYKPAEENGTTFFCEKGTLKMAVEPDAPLVAYFANPGRGR
                     CVFEVPKMQTNEEQFSTGVIDGFVDTIQGKKENPVPGEEGRKALKVVLACLESAASNR
                     KVKIEEVK"
     gene            1535..2497
                     /locus_tag="PLW82_02280"
     CDS             1535..2497
                     /locus_tag="PLW82_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ46132.1"
                     /translation="MKLGLFNVLFSHLSWEEALDKMKAFGIEAVEVGTGNYPNDAHCK
                     PADLLKDKDARMKFVKAAEDRGIMISALSCQGNPLNPNKELAEKHHNTWRNTVELASE
                     LEIPVVCAFSGCPGDSPDAVRPNWVTCAWPNEYSETLKWQWEEVAIPYWQKEAEFARS
                     KGVKIAFEMHPGFLVYNPRTLLKLREAAGDNVGANFDPSHLIWQGIDPVEAIRYLGNI
                     IYHFHAKDTYLDKANIAKNGVLDTASYTNLRDRAWTFRSVGYGIGEKPWRDIITALRL
                     VGYDYVLSIEHEDPLASVDKGLKKSAELLKSIILAEPPAQAWWI"
     gene            2695..3441
                     /locus_tag="PLW82_02285"
     CDS             2695..3441
                     /locus_tag="PLW82_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46133.1"
                     /translation="MRRLLAVVLTLVFLGAFALAEDPFEGLELKLAYDFDFWDEDDTT
                     VGTSEDAYRFITDSQAGVSVLFSGVRKGAYKDEGKIEGLAFKHESIWEAGKPKNYVAF
                     VLHQRHDFNPYGYDEFTGAKYFTFYVDLSGYSKDFEVYPMIYEQDIRDDGSRAGISCL
                     AVKGGATFYMKSADGTITSGKTLENWVKIPKGFVGRVYMPLDAYVPIWGTADENGKFD
                     GLEVIKYRFSIIDIGAKGEYVLFDEFGFLL"
     gene            3503..4783
                     /locus_tag="PLW82_02290"
     CDS             3503..4783
                     /locus_tag="PLW82_02290"
                     /inference="COORDINATES: protein motif:HMM:NF028444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HOJ46134.1"
                     /translation="MKGLIVLLLIALMCGGTAVAKDKAKPKITGAFWQLHSGFFGRGE
                     AYFEEQVELMHKAGIDTIIIQDTAGDNSTTYARSNQVKIKVDGPFAPGTYELLVRKLP
                     VTISEIKSDAQLSYEIVSPEPSPFLGDFGYKLLDGDYSIYGSVGWLEDGESTEVRINI
                     ELKDTANYLDIHFLTLNGNIEREEVLFAGKTGMLTLVNDELTQILEAAEKRGMSVWLG
                     TKFSGTWWGDDFDGGKEIEDNIKTAEALERYYGHYKCFVGYYIPHEFRPDWRPQYNIK
                     FYKELAERLKAYNKPIAIAPFFRVEMPFAAHEKFWREFLQEVDIDVLMLQDGVGSDIS
                     RIEKILPHYEMVQKVAKENGIEFWSDLEVFHVEVPDGPAQPADIKKVTRQLETQAPYV
                     DRIVIFDWLHYMSPLAGDKAQALYEGYLEYLGGE"
     gene            5198..>5313
                     /locus_tag="PLW82_02295"
     CDS             5198..>5313
                     /locus_tag="PLW82_02295"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOJ46135.1"
                     /translation="MKLKTDIEIAQEAKLLPIEEVAKKIGIDESKLEKYGPY"
BASE COUNT         1483 a         1201 c         1392 g         1237 t
ORIGIN      
        1 tatcattgac atcgctgtag ccggagtttc tcagctgccc catgagctca tcgaggttgt
       61 agcgcagggc acgcaaaacg ttttcgcgga tgtgtccgtt ttcaataata atggtagggc
      121 ggccggcgat ccaggtccgt accttttcgc ttttcaggct gattgtggag atcaaaaact
      181 ctagaacgcc taagagagca atgggataga tcgcgtgagt caggggcatg ttcacgtttc
      241 cgatgggcag gacagcaagt tcggcgatca tgatggcagt taccagttcg tagggttgaa
      301 gctggaccat ctgccttttc cccattaacc tcatagccac taggataatg aagtataata
      361 ttactgttct taccgcaacg aaagctccgg tcaagaaaac aacctccctc agggctattt
      421 tctcctttag gggtgcaggt tattatttgc aggtggagaa ttgtaccttt ggaagtccaa
      481 tggaaaaagc tggaggtatg tctatgaagt tacgcgtagg tgtaattggt actggtgcca
      541 ttgcaaagta tcgtcatctc cctgaatatg cagcaagaga agatgtggaa atcgttgcac
      601 tttgcgacat tatcaaggaa aaagctgata aactggcagt tagatacggc gttgaaagcg
      661 tctacaccga ctacaaacag atgattgaaa acgaagaact ggatgcagta agcgtctgca
      721 ctcctaacta cctacacgcc gagatgtcga tttttgcatt aaaccatggt ttgcatgttt
      781 tggtggaaaa accgatggct acaagcttgg acgaagccaa cgcaatgatc gaggctgcga
      841 aaaagcagga gcgcgttttg atggtaggtc agaaccagcg ccttgcccca atgcacgtcc
      901 tggcaaaaga gatcattgaa agcggtatgc tgggcaaagt caacacgttc agaactgtct
      961 tcggtcacgc aggaccagag cactggagca aaacaggcaa atggttcttc gacaaaaagc
     1021 aggcttttgc cggttccctg gcagaccttg gcgttcacaa aatggacctt attcaattca
     1081 tactaggcga tgttgatgaa gtggcctgct tcatcgatac tttggagaaa aaagactgcg
     1141 gcgtcgacga caacgcagta gctgtcctcc gcttgaaaaa cggcattatg ggcactgtgg
     1201 agaccagctg gacctataaa ccagccgaag agaacggaac caccttcttc tgcgagaagg
     1261 gtaccctcaa gatggcagta gagccagatg ctcccctcgt agcttacttc gcaaatcccg
     1321 gccgcggtag atgcgtcttt gaagtaccta agatgcagac caacgaagag cagttctcta
     1381 ccggagtcat cgatgggttt gtagacacca tccaaggcaa gaaagaaaac ccagttcccg
     1441 gagaggaagg cagaaaggct cttaaagtag ttctcgcctg ccttgagtcg gcagcttcca
     1501 accgcaaggt taaaatcgag gaggtaaaat aaaaatgaag ttaggcctct ttaacgtttt
     1561 gttcagccat ctgtcctggg aagaggctct ggacaagatg aaggcgttcg gcatcgaagc
     1621 tgttgaggtc ggaacgggaa actatcctaa tgatgcacac tgcaagccag cagacctcct
     1681 caaagataaa gatgctcgca tgaagttcgt gaaagccgct gaagacagag gcatcatgat
     1741 cagcgccctc agctgtcaag ggaaccctct gaaccccaat aaagagcttg ctgaaaagca
     1801 ccacaacact tggagaaaca cagtggagct cgcaagcgaa ctggagattc ctgtagtttg
     1861 cgccttctcg ggctgccccg gcgactcacc tgatgcagtg agacccaact gggtaacctg
     1921 tgcttggcct aacgagtaca gcgagactct caaatggcag tgggaagagg tagccatccc
     1981 ctactggcaa aaagaagctg aatttgccag atcaaaaggc gtcaagatcg cttttgagat
     2041 gcaccctgga tttttggtat acaacccaag aactcttctg aagctccgcg aagctgcagg
     2101 agataacgtc ggtgccaact tcgatccatc gcacctcatt tggcagggaa tcgatccggt
     2161 agaggccatc cgctaccttg gaaacatcat ctatcacttc cacgccaagg acacctacct
     2221 ggataaagcc aacattgcca agaacggcgt cctcgataca gcatcctata ccaaccttag
     2281 agatcgtgct tggaccttcc gttcggtagg ttacggcatc ggtgagaagc catggcggga
     2341 catcatcacc gccctcagac tggtgggtta cgactacgtt ctcagcatcg agcacgaaga
     2401 cccactggca tctgtggaca aaggcttgaa gaagtctgca gaactcctaa agagcatcat
     2461 ccttgccgag ccacctgcac aggcttggtg gatttagcaa tttgcgccct accccaaaag
     2521 gtagggccct ttttgtgtgc tttttccctg tgtgtaaagg caagactcct cataaaagca
     2581 tgataaacct agctagcaag cagacatttg ctcatcagat tctgtgaagg aaggtctctt
     2641 ccagaacaga actttaagat tgcaggaaaa tgccagttaa agggaggttt tctgatgaga
     2701 cggcttcttg ctgttgtttt gacgttggta ttccttggag catttgccct tgcagaagat
     2761 cctttcgaag gacttgagct caagcttgct tatgactttg acttctggga tgaagacgac
     2821 acaacagttg gaacctctga agatgcatac agattcatca ccgattccca ggctggagtg
     2881 agcgtgctct tttccggcgt gcgaaaaggc gcttataaag atgaaggcaa gatcgaagga
     2941 cttgctttta agcatgagtc catctgggaa gcgggaaaac ccaaaaacta cgtagcgttt
     3001 gtactgcatc agcgccacga cttcaatccc tacggctacg atgagtttac aggtgccaag
     3061 tacttcacct tctatgtgga cctttcaggt tacagcaagg actttgaagt ctatcctatg
     3121 atctacgagc aggacatccg tgatgacggc tcgagagcgg gtatctcgtg ccttgcagtt
     3181 aagggcggag caacgtttta catgaaaagc gccgatggca ccattacatc agggaagacc
     3241 ttggaaaact gggtgaagat ccccaaaggt ttcgtgggca gggtatacat gccccttgat
     3301 gcctatgtac cgatctgggg gactgcagat gaaaacggca agttcgacgg ccttgaagtg
     3361 atcaagtaca gattctccat catcgacatc ggtgcgaaag gggaatacgt acttttcgac
     3421 gagttcggct ttctccttta aaactcagta taagtagtgt agtagcagaa agaccagagc
     3481 acacttgcag gaggggaaac aggtgaaggg gttaatcgta cttcttttga ttgcactcat
     3541 gtgtggcgga acggctgttg caaaagataa agcgaaacca aagatcaccg gagcgttttg
     3601 gcagcttcat tctgggtttt tcgggagagg cgaagcatac ttcgaagagc aagtggagtt
     3661 aatgcacaag gcgggtattg acactatcat cattcaggat acggcgggag acaactcaac
     3721 aacttacgcc agatccaatc aggtaaagat aaaggtggac ggaccgtttg cgccgggaac
     3781 ctatgaactt ttggtgagga agcttccggt taccattagc gagatcaagt ctgatgctca
     3841 gctttcttac gagattgtct cgccagagcc atcgcctttt ttgggggatt tcggctacaa
     3901 gctgctcgat ggcgactact cgatctacgg ttctgtgggc tggctagaag acggcgagtc
     3961 tactgaggtg cgaatcaata tagaactaaa agacactgca aactacctgg acattcattt
     4021 tctcacccta aatggaaaca tagaaagaga agaagtgctt tttgcaggaa agaccggcat
     4081 gcttacactg gtgaacgacg agcttacgca gatcctcgaa gctgcagaga agcggggcat
     4141 gagtgtgtgg ttgggaacca agttctccgg cacttggtgg ggagatgact tcgatggagg
     4201 taaggaaatc gaagacaaca tcaaaaccgc agaagctttg gagaggtact acggccacta
     4261 caagtgtttt gttggctact acattcccca cgagttcagg cctgactggc gtccccagta
     4321 caatatcaag ttctacaagg agctagcaga gcgcctcaag gcctacaaca aacctatagc
     4381 tatagccccg ttcttccgcg tagagatgcc gtttgcagct cacgagaagt tttggcggga
     4441 gtttctgcag gaagtggata tcgacgtctt aatgcttcaa gacggggtag gctcggatat
     4501 cagccgcatt gagaagattc tcccccatta cgagatggta caaaaggtag ccaaagaaaa
     4561 cggtatcgag ttctggtcgg atcttgaggt ctttcacgtg gaagttccag acggaccggc
     4621 gcagcctgct gatatcaaga aggttacccg ccaactggaa actcaagccc cgtatgttga
     4681 tcggatcgtt atcttcgact ggctgcacta catgagtccg ctagcaggcg acaaagcaca
     4741 agccctatat gaaggttatc tggagtactt gggaggagag tagggttaga ggtgtaaagc
     4801 aaagaagttt tggaaatgtt ctcctccgaa ggagcggtac agcacctcag ggcttacgaa
     4861 gtataaaact cgttaaaagg tttttcccac gaatagtagc acactaaagc accaaccggt
     4921 tacggattgg tgcttttcat cagttttgtt ctagctacag gatggaagac catcaagtta
     4981 agagcagtag atctttaccc aaagattttg cttccgagag gtttaatgga gcaggagttg
     5041 gcgtagaaag tcgagctaaa aactggttgc gatgggtttt agtagaaagg tgcagaaatc
     5101 ctcattgtta ctgctccgag aatacaggag ttcaattgcg agctggagaa atctgacact
     5161 aaatgcgaag tgtttggtaa aaaacagcaa gggagagatg aagttgaaga cggatattga
     5221 gattgctcaa gaggcaaagc tcctgcctat agaagaagtg gcaaaaaaga tcggtatcga
     5281 tgagtccaaa ctggaaaagt acggccctta caa
//
LOCUS       DAOSDA010000061         5268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9889_length_5268_cov_3.214080, whole genome shotgun sequence.
ACCESSION   DAOSDA010000061 DAOSDA010000000
VERSION     DAOSDA010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9889_length_5268_cov_3.214080"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLW82_02300"
     CDS             complement(<1..1171)
                     /locus_tag="PLW82_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ46136.1"
                     /translation="MKTLKFGLKALSLVLVPVLLSSSLYADDFVSRKVAEFQGDVENI
                     RKELETAKTQMKRLDYGKIEGILAEICAKLDSSLQPEKTEHEATVILAEVNGLLRDIR
                     PMMEESLPVETRGIWVDNVTMRQLRSRKDVEEFFADMASINVNLVIVDAFNEGAATYP
                     SKVAPEHPESQMYGGDKLRDIVEIAHSKNIEVHALITTFGIGRAGIAHFLDEKMSWLD
                     KHKDGKYDGTGGTYWLSPALPEVREYLLSILKELVGEYNVDGVQLDYVRYDFDFGYHE
                     YSRELFQKLFGIDPLDIKFEGDQNNFILFKTQFVTSFVERAFFELKAIKPDVLLSAAA
                     LSPYTWYKLTLGQDWAGWAYNRNISFVCPMSYTADLVNYQNLALSELRTLKGTSYA"
     gene            complement(1587..2636)
                     /locus_tag="PLW82_02305"
     CDS             complement(1587..2636)
                     /locus_tag="PLW82_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HOJ46137.1"
                     /translation="MSTMKAFVYHGPEKMSLDIVPKPKIQKPTDAIVKVTTSTICGTD
                     KHIRHGGLPEVEPGRIIGHEFCGIVEEVGPAVTRFKPGDRVAVSCVTQCMECYYCRRG
                     MYSQCVDGSWIFGYMIDGCQADYVRVPYADSGMHLIPEELEDEDVLFVGDILSTGYFG
                     AENGKIQPGDTVAVFGSGPVGMCAMATARLFGPATIVAVDIDDSRLEFAKKNGWADVG
                     LNPNKVDVPQALRDMTEGRGADVTIEANGFEPTFKGAINSIRPGGTVSIIGVFEKPQI
                     VEMNKLWIKNITIAMGLVNANRIPELINLIKAGKIDMRPLITHTLPLSQVAEAYDIFE
                     ERRDGAIKVVLKADA"
     gene            complement(2798..3217)
                     /locus_tag="PLW82_02310"
     CDS             complement(2798..3217)
                     /locus_tag="PLW82_02310"
                     /inference="COORDINATES: protein motif:HMM:NF021568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HOJ46138.1"
                     /translation="MVSGELLQSQGKLDERARRKLQHELKIYIEQYFKSKLGKGSDHT
                     RVTIWEDLVVIRGEGFLTDPERFLVETPEGLRIVNSGRMQVCKQHAQDNVPYIENLLT
                     AKVLHQSFLVDAEKDFWMHVLVLDRTVTQESSAKHNR"
     gene            3625..4506
                     /locus_tag="PLW82_02315"
     CDS             3625..4506
                     /locus_tag="PLW82_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOJ46139.1"
                     /translation="MYFATFVQNGKEAVGVLSKEQKKVTELSSILGRRVGSLLEFIEE
                     ASDRDVETIRDYVEKGTDEGASLESVNLLAPIPVLKRNAFCLGLNYKEHVKESQSVRS
                     LDKLPEHPVFFSKAAIDVIGTGMEIDSHPDLTSEVDYEVELAVVIGKAGKNIPEESAY
                     DHIFGFTILNDITARDIQRRHFQWFKGKSLDTFTAMGPYLVHKSVFDLPLELDVKSFV
                     NGELRQNSNTKHMIFSIPRIISELSQSLTLMPGDVISTGTPEGVGMGFKPPKYLKRGD
                     VVECYVEGIGTLRNVVR"
     gene            4713..>5268
                     /locus_tag="PLW82_02320"
     CDS             4713..>5268
                     /locus_tag="PLW82_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ46140.1"
                     /translation="MLLKRIKGNYVFRGVAEVQYKPCYLQLKKEEFDEKIEAGKKHLT
                     QCTLCPRECGVDRTKELGYCQAGVEVKVSSFGPHFGEERELVGRRGSGTIFFGHCNLR
                     CVYCQNHQLSFGGEGSKYSVEQLAEMMLIVQDYYLCSNINLVSPTHYVPQILEAVYLA
                     SQKGLELPIVYNCGGYEKVDTLKLL"
BASE COUNT         1284 a         1206 c         1393 g         1385 t
ORIGIN      
        1 aggcatatga ggtgccctta agggttcgga gctcgcttaa tgcgaggttc tggtagttga
       61 caaggtctgc ggtgtaggac atagggcaga caaagctgat gttgcggttg taggcccagc
      121 ccgcccaatc ctgacctagt gtcagcttat accaggtgta cggtgacaga gccgctgcag
      181 aaaggagtac atccggttta atggctttca gctcgaagaa tgccctttct acgaagctgg
      241 taacaaactg agttttgaag aggatgaagt tgttctggtc tccttcgaac ttgatgtcca
      301 gtggatcgat accgaaaagc ttctggaaaa gctcccttga gtactcgtga tagccgaaat
      361 cgaagtcgta gcgcacatag tccagctgaa cgccgtcgac gttgtactcg ccgactagtt
      421 ccttcaaaat ggagagcagg tactctcgga cctctggaag agccgggctt agccagtagg
      481 ttcctccagt tccgtcgtac ttgccgtctt tgtgtttgtc cagccaggac attttctcat
      541 cgaggaagtg agcaatgccc gcacggccga tgccgaaggt ggtgatcagt gcgtgaactt
      601 cgatgttttt ggagtgagcg atctccacga tatccctcag cttgtcgcca ccgtacatct
      661 ggctttcagg gtgctccggt gcaaccttgc tggggtaggt ggcagcgcct tcgttgaagg
      721 catcgacgat aacaagatta acgttaatcg aggccatatc tgcaaagaac tcttctacat
      781 ccttacggga gcggagctgc ctcatggtca cgttgtccac ccaaattcct cttgtttcaa
      841 cgggaagcga ctcttccatc ataggcctga tgtccctgag gagtccattt acctctgcta
      901 ggatcacggt agcttcgtgc tcggtctttt ccggctggag ggaagagtcg agcttggcgc
      961 aaatctccgc aaggatgcct tcgattttgc cgtagtccag acgcttcatc tgagttttgg
     1021 cggtctctag ttctttgcgg atgttttcaa catcgccttg gaactctgca actttccgcg
     1081 agacaaagtc gtcggcgtag agcgaagaag aaagcagcac cggcaccaac actagagaca
     1141 gggctttgag accaaactta agagttttca aaggtttaac ccccttcttg gaattgatga
     1201 atgaggtgtg tttccggttt tgtggatgcg aaggtttatg tatgtaaccg ggctgttaga
     1261 ccgatcgggc gtttacgtac tgcctaagtt ttcacaccta cagttctcag cagaaaggag
     1321 ttttccttcg ctaaacttgt tttttgttcg gaaaattccg agtggaagag ggaagtgcga
     1381 gatataagct ttattgaggt tggatttgga acttccggag acagagcgtt ttttcggaca
     1441 aacccttgct ctatacagct atgctttgct ttggaggcct gcaaaaaagg gccccctctg
     1501 tggtaaacag tcgtcttggc gtaactgtct agcgcaaagg gagcctatag gaacggaaca
     1561 aaagatccgg cgatttcact ttgaagctag gcgtctgcct tcagcaccac tttgatggca
     1621 ccatcgcggc gctcttcgaa gatgtcgtag gcttcggcta cttgagacag aggcagcgtg
     1681 tgcgtgatca gtggcctcat gtcgatcttt cccgctttga tcaggttgat cagttcgggg
     1741 atgcggttgg cgtttaccaa gcccatggcg atggtgatgt ttttaatcca gagcttgttc
     1801 atttcgacga tttggggctt ttcgaagacg ccaatgatgg aaacagttcc tccgggtctg
     1861 atgctattga tcgcaccttt gaacgttggt tcgaaaccgt tggcttcaat ggtgacgtct
     1921 gcaccgcggc cttcggtcat gtcgcgcagt gcttggggaa cgtccacctt gttggggtta
     1981 aggcctacgt ctgcccagcc gttctttttg gcaaactcca agcgggagtc gtcaatatcc
     2041 acagccacga tagttgcagg accaaagagg cgtgctgtgg ccattgcgca catgcccaca
     2101 ggaccggaac cgaagacggc tacggtgtcc ccaggctgga ttttgccgtt ttcagcacca
     2161 aagtaaccgg tggaaagaat gtctcctaca aacaggacat cctcatcttc cagttcttct
     2221 ggaatcaggt gcatgcccga gtcggcgtag gggactctga cgtagtcagc ctggcagccg
     2281 tcaatcatgt agccgaagat ccagctgccg tctacgcact gggagtacat acctctgcgg
     2341 cagtagtagc attccataca ctgggtaacg caggaaaccg caactctgtc gccgggctta
     2401 aacctggtta ccgcagggcc gacttcttca acaatgccgc agaactcgtg tccgatgatt
     2461 ctgccgggct ccacttccgg gagaccgccg tggcggatgt gcttgtcggt tccgcagata
     2521 gtggaagttg ttacctttac aatggcatcg gtgggttttt gaatttttgg tttgggcaca
     2581 atatcaagac tcatcttttc gggaccatgg taaacgaaag ctttcattgt tgacatacaa
     2641 tcaccctcct aagtcgtcag ctttccttgc agtaagataa gtcaaagccg tggtgccttt
     2701 tttatccctc ctttctctca aaaaaataga ccgagtctgt ggctttctgg ccagagactc
     2761 ggtctatctc agggcaacgg cttcgtttcc cttcaatcta ccggttatgc tttgcagagc
     2821 tttcctgggt tactgtgcgg tcgaggacta gaacgtgcat ccaaaagtct ttttctgcat
     2881 cgactaaaaa cgattgatgg agtacctttg cggtaagtag gttttctatg tagggtacgt
     2941 tatcttgggc atgctgttta cacacctgca tgcgccccga gttgacaatg cgcagacctt
     3001 caggtgtttc gacgaggaat ctttccgggt ctgtaaggaa gccttcgcca cggatgacta
     3061 ccagatcttc ccatatggta acccgggtat ggtccgatcc tttgccgagt ttggacttga
     3121 agtactgttc aatatagatc ttcagctcgt gctgtagttt tcttcgtgcc cgctcatcca
     3181 gctttccctg actctgtaga agttcaccag acaccatttt tcgtctcctt ccttagggaa
     3241 cttaccagag taccaaagtg cggaaaaaaa caaaaccagt atttgatggc ccgagaaaaa
     3301 cacgagctgc caaatactgg tattttagcg agccacctaa cggtttctcc ctaaaccacc
     3361 ttgactccga acattgaaaa gattgtcttt ttttatcata gtgcaataga atctgaatgt
     3421 caacatctcg gagtgatacg gacaagctga atgctccaaa accctcctat gcttgcagga
     3481 tagttggtct gtagagtgaa tcttaaccgg taactgcagc gttttaacgt agagccgttt
     3541 ggtcttttat gtagatgaga gaagcgcgca aagcgggtgt tgtttgaaaa agagaaatga
     3601 tgcaattaat tgaggtgaga ggcagtgtat tttgcaacct ttgttcaaaa tggtaaagaa
     3661 gcagttggtg ttctgagtaa ggagcaaaag aaagtgactg agttgtcgtc gatacttggg
     3721 agacgagtag ggtcgctgtt ggagtttata gaggaagctt ccgatcggga cgtagagacg
     3781 attagagact acgttgaaaa aggtactgac gaaggcgctt ctttggaatc ggtaaatctc
     3841 cttgcgccca ttccggtgct caagcggaat gctttctgcc ttgggctgaa ctacaaggaa
     3901 catgtgaagg agtcccagtc ggttcggagc ttggacaaac ttccggagca tccagtgttc
     3961 ttcagtaaag cagcgattga tgttatcggt acggggatgg agatcgactc ccaccccgac
     4021 cttactagtg aggtcgacta tgaagtagag ctggcggtcg ttattgggaa agcggggaag
     4081 aacattcctg aagaatcagc ctacgaccac atcttcggct tcactattct aaacgacatc
     4141 accgcacggg atattcagag gcgtcacttc cagtggttta aggggaaaag cctggatacg
     4201 tttaccgcta tgggaccgta tctagtacac aaaagcgtct ttgaccttcc actggaactg
     4261 gatgttaaat cgtttgtaaa cggagagcta aggcaaaact ccaacaccaa gcacatgatt
     4321 ttttcgatac cgaggattat cagcgagctg tcccagtcgc taactttgat gcctggagac
     4381 gtaatctcca ctggaactcc tgagggagtg gggatggggt ttaaaccgcc gaagtatttg
     4441 aaaagaggcg atgttgtgga gtgttatgta gaagggattg gaacgctgag gaatgtggtg
     4501 aggtaactta tggcgtgcaa gaagtgccaa agcataggca cgatgtggcg gtgagaacgc
     4561 acgacttggg ttctaaggca ttgcaacggt gattgagcca gacacatgtt tttagccagt
     4621 tactagccaa acgatttggc agaagtgcgt tttcacaatc ctttctgtta tacgtcaaca
     4681 taggtttctt acgtaaagca ggaagaactg ctttgctctt gaaacgtata aaaggcaatt
     4741 atgtcttcag gggggtggct gaagtgcagt acaaaccctg ttatcttcag ctgaagaaag
     4801 aagagtttga cgaaaagatc gaagctggta agaagcatct gacgcaatgc actttgtgtc
     4861 cgagagaatg cggagtagat cggacaaagg agttggggta ctgccaagca ggtgtggaag
     4921 ttaaagtgtc tagcttcggt ccgcacttcg gagaagaaag ggagcttgtc gggagaagag
     4981 gctcaggaac cattttcttt ggccattgca atctccgctg cgtttactgc caaaaccacc
     5041 aactaagctt tggcggagag ggttctaagt actctgtaga acagctggca gagatgatgc
     5101 taatcgttca agactactat ctctgctcaa acatcaacct ggtcagtccc acccactacg
     5161 ttcctcaaat tctagaagca gtctatcttg catcccaaaa gggattggaa ctacctatcg
     5221 tctacaactg tggcggttat gaaaaagtag acacgttgaa gctacttg
//
LOCUS       DAOSDA010000062         5251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_9929_length_5251_cov_3.760393, whole genome shotgun sequence.
ACCESSION   DAOSDA010000062 DAOSDA010000000
VERSION     DAOSDA010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9929_length_5251_cov_3.760393"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLW82_02325"
     CDS             complement(<1..850)
                     /locus_tag="PLW82_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOJ46141.1"
                     /translation="MRVLIYTRVSTQGQKEGYSLESQKSACLKKAEELSAEVIYHLED
                     TYPGAELERPALNQLRELVQSRQIDAVICLDPDRLSRNLADLLLLTSEMDRSNVELVF
                     VNFTWESTPLGRLFLEMRGAIAEFEHALIRERTLRGKRQKAREGRLPSFVEPYGYRFN
                     PKDDCLNVVQREAAVVSYIFREYNRGCNSGRILAEKLNKQRIPAPSGAKWHPSTVLRI
                     LSNRTYLGVLDVGGIEIEVPKLIGEEDFAKAQEIKALNRRRSLRRTKKEYLLQNLLTC
                     SLCGSSL"
     gene            complement(961..2214)
                     /locus_tag="PLW82_02330"
     CDS             complement(961..2214)
                     /locus_tag="PLW82_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ46142.1"
                     /translation="MTVAISSATELIEKRLDGHNKRVAYIAYRLAAQLEMAFDDLIDV
                     IISGLIHDVGIIGIKNTASLFELDKEDILHAEVGYRLLRTIPELSTIALIVRGHHGEY
                     QRVAEREPRREVFLSQLLSLADRVDVLLDYESDPLSQIDGVLEALDRRKGTRFHPEIV
                     EAFAELAKVEAFWFDLFTMNFVDRLVSIANERSLYLDDDGLLRFGRFFSHVIDFRSHF
                     TSTHSSGVAAIAVFLAEKSGFRGRELTLMKIAGYLHDVGKLAVPNYILEKDGYLTKDE
                     LNVIKSHTYYTKVILEKIRGFEEVALWAALHHQKLDGSGYPFHLKGEEIPLGARIITV
                     ADIFTALNEDRPYRKGVSENETAMILTEMAQKGWIDAAIVNLAISHYSEIRDLCLREQ
                     KLAGEKYRDFWGVSTKTGMVHLQSE"
     gene            complement(2513..4150)
                     /locus_tag="PLW82_02335"
     CDS             complement(2513..4150)
                     /locus_tag="PLW82_02335"
                     /inference="COORDINATES: protein motif:HMM:NF027473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4855 domain-containing protein"
                     /protein_id="HOJ46143.1"
                     /translation="MFLLVLLLTFLVSVGVNAEPTNLALGKPYTINIEAESRYRDFGN
                     LEATDGKRGLNSIFNAAWQGHAQKEYRILVLDLGATYEIDRVEMGFIQDQPSNVWVPN
                     SLSLAVSDDGINWQVVNEEIYEGLSSENQTAIRTIAANQATGRYVAVFFGVNFWVFFD
                     EIEVYGNPVSLGNDGESNLANVDFDALYRGFLSTVNVDYFKAVEGDLMRVSEETGWAH
                     DILLVYDQASGIVWNEHYALPYVAYLDEDLQPKDWFFDTYLFLALTAPGGYNFAAYSV
                     TDKTHWDWWLDRVFADGQGFDAFNKAVITVGQALGDEEYKAKVIVMIPYPTELPSLPF
                     DPHNPGKLNPDVVGMEEAARNRLEVVKEYIDEVLTRWNEKNYSRLELIGFYWLSENLD
                     SAGFDRFYLPDIGAYVRTCGLKFYWIPWYMAPGYREWRELGFDAVMMQPNYMFTDIPN
                     KSRFVFAAAEAKKYQLGLEFESSSKILRSAEDRELYYDYLRAANTLGYGNDILRGYYQ
                     DVTLYYQAAHSKDPEVRRIYDATYEFVKGTYTGKFED"
     gene            complement(4211..>5251)
                     /locus_tag="PLW82_02340"
     CDS             complement(4211..>5251)
                     /locus_tag="PLW82_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOJ46144.1"
                     /translation="TASNGTELAFRHPNGNWDVRRTAAYKERFAIGKTYRVKVAVTGN
                     YYFYFINDELMFAAYETGFRESGIFGLHVNGCRVAFDNIVIESYNSADYKEFEEMVAK
                     EIRPILPRIVAHRGNSSVAPENTLAAIQSAIDVGADMVEIDVYPTKDGEIVLLHDGTL
                     DRTTNGTGSVSNYTLAELKELDAGSWKSLRYRGEKIPTLREALELAKDKIRVVIELKT
                     TGQERKVLEIIDELGMRDQVAIISFHTTAVRTFRQIAPDIPAAILIGSASSYAQIENI
                     AKQANTTILDLSYSLIDKDSAVYFIERGYSLWAWTVNNEAVMERMAECGVVVLTTDEP
                     AAALKLLRTSNK"
BASE COUNT         1225 a         1371 c         1222 g         1433 t
ORIGIN      
        1 taagagacga accgcacaga ctgcaggtaa gcaggttttg caaaaggtac tccttctttg
       61 tcctgcgcag cgatcgcctg cggtttagtg ctttgatttc ctgggctttg gcgaagtctt
      121 cttcgcctat gagcttcggg acctcaattt caatccctcc tacgtcaaga acgcccaggt
      181 atgttctgtt cgataagatg cgtagaaccg tcgacggatg ccatttggca cctgaaggag
      241 cagggatcct ctgcttgttc aacttctctg caaggatcct gccgctgtta cagcctctat
      301 tgtactccct aaagatgtag gaaacaactg cagcttcccg ctggaccacg ttcaaacagt
      361 cgtctttggg gttgaaccgg taaccgtagg gctctacaaa actcggcagg cggccttccc
      421 ttgccttttg gcgttttccc ctcaaggtgc gttcacggat tagtgcgtgt tcgaactcgg
      481 caatagcgcc cctcatctcc aaaaagagcc tgccaagagg tgtagactcc caagtgaagt
      541 tgacaaacac cagctccaca ttggaacgat ccatctcgct tgtcagaagc agtagatctg
      601 caaggttccg gctgagcctg tcgggatcaa ggcagatgac agcatcgatc tggcgactct
      661 gcaccaactc ccgcagctgg ttcaatgcag gtctttctag ctccgctccg gggtaggtat
      721 cttctagatg gtagatgact tcagcgctga gctcctcggc cttttttaag cacgctgact
      781 tctgactttc caagctgtag ccttccttct gtccctgagt gcttaccctt gtgtagatca
      841 ggaccctcat ggaaatcctc ctcaagacgc agatctgtaa agagagacca ggcttttagt
      901 gcctggtctt cgttttccgt ttttatccag gtgatttgca tacgatcccc cgataaaaac
      961 ctattcactt tgcagatgta ccataccagt ttttgtgctc acaccccaaa agtctcggta
     1021 tttttctcca gctagcttct gctccctgag gcagagatcc ctgatctcgc tgtagtggct
     1081 gatcgccaaa ttgacaatag cagcgtcaat ccagcctttc tgtgccatct ctgtgagtat
     1141 catagccgtc tcgttctccg agacgccttt tctgtaaggc ctgtcttcat taagagctgt
     1201 aaagatgtcg gcaacggtga taatccttgc acctaagggt atctcttccc ctttaaggtg
     1261 gaatgggtag ccggagccgt cgagtttctg gtggtgcagc gctgcccaaa gtgcgacttc
     1321 ctcaaagccc ctaatctttt ccaagatcac cttggtgtag taggtgtggc tcttgataac
     1381 gttcagttca tctttggtta ggtagccgtc tttctccaag atgtagttgg gtacagccag
     1441 cttccctaca tcgtgaagat acccggcaat cttcatcaga gtcaattcac ggcctcgaaa
     1501 tccgcttttt tcggccaaaa acaccgcgat ggctgcaact ccgctggagt gggttgaggt
     1561 gaagtggctc ctaaagtcaa tgacatggct gaaaaaccgg ccgaatctta acaggccgtc
     1621 gtcgtctaag tagaggcttc tttcattggc aatgcttacg agcctgtcca caaagttcat
     1681 tgtgaaaagg tcgaaccaaa aagcttctac cttcgctagc tcggcaaagg cttctactat
     1741 ttccggatgg aacctcgtgc cttttctgcg gtcgagggcc tccaaaactc cgtcgatctg
     1801 actgagagga tcgctctcat agtccagaag gacatcgaca cggtctgcca agctcagcag
     1861 ctgggacaaa aaaacctccc gcctaggttc cctctctgca accctttggt actcgccgtg
     1921 atgacctctg acgatcaacg cgatggtaga tagctccggt atagtcctca gcagcctgta
     1981 cccaacttca gcatgaagaa tgtcttcttt atccagttca aagagactag ctgtattttt
     2041 tataccgatg attcccacat cgtggatcaa gccactaata ataacgtcta tgaggtcatc
     2101 aaaagccatt tccagctggg ctgccagccg ataggcaata taggccactc tcttgttgtg
     2161 tccgtcgagc cgtttttcaa taagctcagt tgcactagaa atcgccacgg tcatatctcc
     2221 caatggcaaa acgaaacttg tttcgatcgt caacgcctcc tgcacattcc atcaagtatc
     2281 gatcatttga tcatcgccgg caatttagaa catcgcgtat cttgctttat ttactactat
     2341 cgtttcggag atttgtcctg cactagaaaa ccagaaaacc tctgtagatt gttaaaatag
     2401 gcaatcttac ccagaaaagc aaatatgagt ggatgtgatt ttactcacac ccaccaaatt
     2461 gcagacaagc aaaaactctc ggactacgta gaagtctaga agttctgccc ggttaatctt
     2521 cgaattttcc cgtgtaggtt ccctttacaa attcgtaggt tgcatcgtat attcttctaa
     2581 cttcagggtc cttgctatga gctgcctggt agtagagagt tacatcctga taatagcctc
     2641 tgagaatgtc gttgccataa cctaaggtgt ttgcggctct gaggtagtcg tagtagagct
     2701 ctctgtcttc agcgctgcga agtatctttg acgaagactc gaattccaga cccagctggt
     2761 actttttggc ctcagctgca gcaaacacga acctggactt gttgggaatg tcggtaaaca
     2821 tgtagttcgg ctgcatcatt actgcatcga agcccaactc tctccactct ctgtaacctg
     2881 gagccatgta ccagggtatc cagtagaact tcaggccaca cgtccgtaca tatgccccaa
     2941 tatccgggag gtaaaacctg tcaaaacctg cggaatcgag gttctctgaa agccagtaga
     3001 acccgatcag ttcaagtctg ctgtagttct tctcattcca ccgtgtaagg acttcgtcga
     3061 tgtactcttt gaccacctca agcctgtttc tagctgcttc ttccatccca acgacgtccg
     3121 gattcagttt tcccgggttg tggggatcga aaggcaagct gggtagctct gttggatagg
     3181 gaatcatcac gatgaccttg gctttgtact cttcatctcc cagtgcttgc ccgacagtga
     3241 taaccgcctt gttgaacgca tcaaacccct gtccatcggc aaacacccga tccagccacc
     3301 aatcccagtg ggtcttatcg gttacggagt atgcagcgaa gttgtaccct ccaggagcag
     3361 tcagagccaa gaaaaggtag gtgtcgaaga accagtcctt tggctgcaga tcttcatcta
     3421 ggtaagcgac atacggcagg gcgtagtgtt cgttccaaac gatgccagat gcttgatcgt
     3481 agaccagaag aatgtcgtgg gcccaaccgg tctcctctga tactcgcatc aggtctcctt
     3541 caactgcttt aaagtagtcg acgttaacag tgctcaaaaa tccccggtac agtgcgtcga
     3601 agtcgacgtt tgccaggttt gattcgccgt cgttgccgag ggagacggga tttccgtaga
     3661 cttcaatctc gtcaaagaag acccaaaagt tcacaccgaa aaacactgcc acgtacctac
     3721 ctgtagcttg attagcggcg atggtacgga tagctgtttg gttttccgaa gacaggcctt
     3781 cgtagatctc ttcgtttacc acctgccagt ttatcccatc atccgaaacc gctagagaca
     3841 ggctatttgg cacccagacg ttggatggct gatcctgaat gaaacccatc tccactctgt
     3901 cgatctcgta agttgccccc agatcaagca ccaaaatgcg gtattccttc tgggcatgtc
     3961 cttgccaggc tgcgttgaag atgctattta ggcccctttt accgtctgtg gcttctaggt
     4021 tcccgaagtc tctgtacctg ctttctgctt cgatattgat ggtatatggc ttgcctagtg
     4081 ctagattggt aggttcagca ttgaccccga ccgaaaccag aaacgtcaaa aggagtacta
     4141 gtaaaaacat tcgttaacct cctgaaaatg gtgataatca gcacattggg cgggtgatcc
     4201 cgcccaatgt ttatttgtta gaagttctta aaagttttag cgccgctgcc ggctcgtcgg
     4261 tggtcagtac gactacaccg cactcagcca ttctttccat tacagcctcg ttgtttacag
     4321 tccatgccca gagggagtag cctctttcaa tgaagtagac tgcagagtct ttatcgataa
     4381 ggctgtaact gaggtcgagt atggtggtgt ttgcctgttt ggcaatgttc tcgatctgag
     4441 cgtaggagct ggcacttccg ataagaattg cagcagggat atctggggca atctgacgga
     4501 acgtgcgcac tgctgtggtg tggaacgaga ttattgccac ctgatctctc atgcccagct
     4561 catcgatgat ctcgagcacc ttccgctcct gtccggtggt cttcaactca attactacac
     4621 gaatcttgtc tttagcaagt tccaacgctt ctctgagagt aggaatcttc tcccctctgt
     4681 acctcaagga tttccaggaa cctgcatcta gctctttgag ttctgctaga gtgtagttgg
     4741 atacgctgcc ggtaccattt gtggtgcgat caagggttcc atcgtggagg aggacgattt
     4801 caccatcttt agttggataa acgtcgatct cgaccatatc tgctcctaca tcgatagcgg
     4861 actggatggc agccagggtg ttttcaggag caactgagga gttccccctg tgggcaacga
     4921 ttctaggcag tataggcctg atttcttttg ccaccatctc ttcgaactct ttgtaatctg
     4981 cgctgttgta gctttctata acaatattgt caaaagcaac tcggcagccg tttacgtgga
     5041 gaccgaaaat gccgctttct ctaaagcctg tctcataagc tgcaaacatc agctcgtcgt
     5101 taataaagta gaagtagtag ttcccggtga ccgcgacttt gacccggtag gtcttaccga
     5161 tggcaaagcg ttctttgtaa gcggcagtcc tcctgacgtc ccagtttcca ttgggatgtc
     5221 tgaaagcaag ttcagtaccg ttagatgccg t
//
LOCUS       DAOSDA010000063         5133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_10185_length_5133_cov_3.083891, whole genome shotgun sequence.
ACCESSION   DAOSDA010000063 DAOSDA010000000
VERSION     DAOSDA010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10185_length_5133_cov_3.083891"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PLW82_02345"
     CDS             <1..392
                     /locus_tag="PLW82_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46145.1"
                     /translation="NSSKKKQQAWEFIKWITSEEVQLAISNRVMAQIQGAMFLPSNRG
                     AALKLDVDPVVASSWDKALHEATSSVYGLVAPRHRRRYLQMAAQKSILMGVDPETAMR
                     EAADEHNSEIARKQKEYDRYIKQLLGK"
     gene            528..>943
                     /locus_tag="PLW82_02350"
     CDS             528..>943
                     /locus_tag="PLW82_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46146.1"
                     /translation="MKKTLILALVLMLSVLSLAKPLEVWLTGLTNEELSIFREIATDY
                     TKQYGVEVVFTNLSWSDFENRFILAAASGEAPDIGGMGPLFAPELGLRGAVIDLKATF
                     PDFDEVVANLPPGFFTALTYKGAVFGIPYNANITVA"
     assembly_gap    944..953
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <954..1805
                     /locus_tag="PLW82_02355"
     CDS             <954..1805
                     /locus_tag="PLW82_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46147.1"
                     /translation="NDLGISSINTWEEMKKVLPKAQAQGSNMALQFGLTENVYADLNM
                     FMWQRGADDYTPDLSRSGFDTPESIAAFKEYVSFYVDYHIPKEIPFFQAFSTGELFYM
                     LQYQNMYTNLKIAAPQIAGKWSMVQVPGTYQGDTLVRSASAGGAALTIFKTSKMKNEA
                     WNFIKWITSPRIQAEFSVRVMNQIPGSIFIPANREAVLNIDMPKEDLELLLKAADEGK
                     ISTYGLVAPRLRRRYLQFAAQKAILQGEDPEKAIREAADEHNTEIKKKEVEYARFIAN
                     LLKEQGK"
     gene            complement(1845..2897)
                     /locus_tag="PLW82_02360"
     CDS             complement(1845..2897)
                     /locus_tag="PLW82_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46148.1"
                     /translation="MFAFIGLILILLVVFPTHTAAFVPEGAIEAQELYVKSLQKDAGY
                     LTMAPTDARINPYFNNIALWAVVDNPDYTDVVKKWAMWYVEHINDPDKLGVRGSIYDY
                     TVVAGGEVKPTEDYDSSDSYAATFLTLMRRYFDATNDSAFLKELEPALDLVASAIYAT
                     MDPKDGLTYAKDNYRIKYLMDNVEVWQGLKDWAYLLEHVFARTDDAKKATADAAKIYD
                     SLQKFWKGSAFAYAKDEVGRLFGSDTTVFYPDMTAQLMAVALEFTTLEQTEIIWKNFN
                     EKFPKWVLLQHPSSFPWTMMVYAGLKAGDLERVQTYINAVELTYGSMGFPWPWYSSEA
                     GWYLLTLKELPAIETE"
     gene            complement(2942..3841)
                     /locus_tag="PLW82_02365"
     CDS             complement(2942..3841)
                     /locus_tag="PLW82_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOJ46149.1"
                     /translation="MTKKVQGYMWLLLTIFLFSTYEIVGHTLTSSTTTMFVNAFRFLL
                     GGLVLLPFALSYMKKHDVKLTQKDFFRLLVLGLLNAVLSMNLLQLSFKFAPASTSAIL
                     FSTNPLVVAIFSSLLLREKPSRSQVFGLFLGVIGSIFVTGSFEASNIVGPLLALSAAV
                     LFGLYTVAGKDLTMRLGSLVVNSFSFTAGGIINWSLLALKSEPLWQVAGDNWLRLLYL
                     GVFVTGLAYFAYFTGLSHLDTTAGSSVFFVKPVFASILAWIFLSEPITWNKAVGIVII
                     LLAMGFVIARPQKTAASRKEAEA"
     gene            3945..4271
                     /locus_tag="PLW82_02370"
     CDS             3945..4271
                     /locus_tag="PLW82_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46150.1"
                     /translation="MRFENEGGKATFNGKEIKNPFVRLLFGIVCVLVSALILSVILAV
                     LLPVFGRSIDLTVLHYIALVLGVLVIIPTILAGSLVTGIVSGSIELFSSSKSKEKKQT
                     DVKKKK"
     gene            4465..>5133
                     /locus_tag="PLW82_02375"
     CDS             4465..>5133
                     /locus_tag="PLW82_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ46151.1"
                     /translation="MSQSKETKYTVKRGDTLKSIALENNLTVEAILNANPDIADPEDL
                     RSGQSIVLPLLNTSGYSSGDYYTVKVGDTLESIAKEHGTAVDAILLKNPKLIDTDSVE
                     AGETIEIPKAHASQIPPPPATCSGRYYTVVAGDSLYLIAQRNNITLDALLAANPQITD
                     PARISVGQVICVPGTTPSCEGSLYTIRAGDTLFSLAQRYNTTVDAIIRANPGIDPNNL
                     RIGQV"
BASE COUNT         1395 a         1210 c         1249 g         1269 t
ORIGIN      
        1 tcaactcttc gaagaagaag cagcaggcct gggagttcat caaatggatt acctctgaag
       61 aggttcagct ggccatttcc aaccgtgtta tggctcagat ccagggtgcg atgttcctgc
      121 cgtccaacag aggtgctgct ttgaaactgg acgtcgaccc tgtagtggca tcttcttggg
      181 ataaagccct tcacgaggct accagctccg tctacggttt ggtggctcca agacaccgca
      241 ggcgctactt gcagatggca gctcaaaaga gcatcttgat gggcgttgat cctgagaccg
      301 caatgcgcga ggccgcagat gagcacaact ccgagattgc cagaaagcaa aaagagtacg
      361 acagatacat taagcagctt ctgggcaagt aataatctgt tcaccaaggt gccgggaatg
      421 cccggcacct ttttttctac tgtgctccag ataaggaaac agaaggagaa gagaagattt
      481 agaagaatct attaaggggt tttgatttcg aaacggaggg atgttttgtg aagaaaacgc
      541 taattcttgc tcttgtcctc atgcttagtg ttttatcact tgccaagcct ctggaggttt
      601 ggctaacagg acttaccaac gaggaactga gcatctttag agagatagca acagattaca
      661 caaagcagta cggagtggaa gttgttttta ccaatctcag ctggtctgac tttgaaaacc
      721 gtttcattct tgcagctgcc tctggcgaag ctcctgatat tggcggtatg ggcccactct
      781 ttgctccgga gctcggtctt agaggtgccg ttatcgatct taaggcaacg ttccccgact
      841 tcgatgaggt agttgccaac ttgcctccag gcttttttac cgccctcacc tacaaaggag
      901 cggtgtttgg cattccgtac aatgccaaca ttactgtggc attnnnnnnn nnnaacgatc
      961 ttggcatatc ctccataaac acatgggagg aaatgaaaaa ggtactcccc aaggctcaag
     1021 ctcaaggcag caatatggca ctgcagtttg gcctcacgga aaacgtttac gcagatttga
     1081 acatgttcat gtggcaaaga ggcgctgacg attacacccc tgatctgtcg cgttcggggt
     1141 tcgatactcc agagtcaatc gcagctttta aagagtatgt cagcttttat gtagactacc
     1201 atatccccaa agagattccg ttcttccaag cgtttagcac cggagaactc ttctacatgc
     1261 tgcagtatca gaatatgtac accaatctga agatcgcagc accgcaaatc gcaggcaagt
     1321 ggagcatggt acaggtcccc ggaacctacc aaggagatac cctggtaaga tctgcatctg
     1381 caggaggtgc agcgctcacc atcttcaaga cttccaagat gaagaatgaa gcttggaact
     1441 tcattaaatg gatcacatca ccaagaatcc aagcggagtt ttctgtcagg gttatgaacc
     1501 agattcccgg ttcgatcttc attcccgcaa accgcgaagc agtgctgaac atcgatatgc
     1561 ccaaagaaga tctagaactt cttttgaagg ctgctgacga aggcaagatt tcaacctacg
     1621 gtctcgttgc tccaaggctc cgccgccgct acctgcagtt tgcagcgcag aaagccattc
     1681 tccaaggaga agatccggag aaggctatcc gagaagctgc agacgagcat aacacagaaa
     1741 tcaagaaaaa agaggttgag tacgccaggt tcatcgcgaa cctcttgaaa gaacagggga
     1801 aatagacgat taaagggcta acctttacgg ttagccctag ttttttactc agtttctatt
     1861 gcaggcagtt ctttaagagt gaggagatac cagccggcct ctgatgaata ccaaggccag
     1921 gggaagccca tgctgccgta ggtgagttca actgcgttga tataggtttg aactcgttcc
     1981 agatcgccag ctttgagtcc ggcgtagacc atcatagtcc agggaaagct tgatggatgc
     2041 tgcagcagta cccacttagg gaacttttcg ttgaagttct tccagatgat ctcagtctgc
     2101 tccagagtgg taaattccaa tgccactgcc atgagctgtg cggtcatatc ggggtagaag
     2161 acggtagtat ccgagccaaa cagcctgcct acctcgtctt tggcatatgc aaaggctgat
     2221 cccttccaaa acttctgcag gctatcgtaa atcttagctg catctgcagt tgcttttttc
     2281 gcgtcgtctg tcctagcaaa cacatgctct aggaggtatg cccagtcttt caacccctgc
     2341 cagacttcga cgttgtccat caggtacttg atccggtagt tgtccttggc gtatgtcaga
     2401 ccgtctttgg gatccatggt ggcgtagata gcactggcaa caaggtccaa agcaggttcg
     2461 agttctttaa ggaaagcgga atcgtttgtg gcatcgaagt agcggcgcat caaggtcaga
     2521 aatgttgcag cgtaagagtc ggacgagtcg tagtcctcag taggcttgac ctcgcctcct
     2581 gctacaacgg tgtagtcgta gatgctacct cttaccccga gcttgtctgg gtcgttgatg
     2641 tgttcaacat accacatggc ccactttttc accacatcgg tgtagtcggg attgtctact
     2701 acagcccaga gtgcgatgtt gttaaaatac ggattgatcc tggcatcggt tggtgccata
     2761 gtaaggtacc ctgcatcttt ctgcaggctc ttcacataca gctcctgggc ttcaatcgct
     2821 ccttctggga caaatgcggc agtgtgggtg gggaagacta caagcaagat aagaatgagt
     2881 ccaataaagg caaacatcgg gcatcctcct ttttcagtgt tttgccggtc acctgcttgg
     2941 ttcaagcttc agcttcttta cgggaagcag cagttttttg tggccgagct ataacaaagc
     3001 ccatggcaag cagaataatc actataccta cggctttgtt ccaggtgata ggctcgctca
     3061 agaagatcca tgcaagaatc gatgcaaaga ctggtttgac aaagaagacg ctggatcctg
     3121 ctgtggtgtc cagatgggaa agacctgtaa aataagcaaa gtaggccagc ccagttacaa
     3181 acacgcctaa gtacagaagt ctcagccagt tatcaccggc aacctgccag agaggctcgc
     3241 tttttagggc tagtaaactc cagttgatga taccacctgc ggtaaaggaa aagctgttta
     3301 ccacaagact tccgagacgc atggtgaggt ctttaccggc tactgtataa agtccgaaca
     3361 aaaccgctgc gctaagggct agaagtggtc cgactatgtt agacgcttcg aaacttccgg
     3421 taacgaaaat tgaaccaatg acgcccaaaa acagaccaaa gacttgagac ctgctcggtt
     3481 tctccctgag gagcagggaa gagaagatgg caacaacaag agggtttgtg ctgaaaagga
     3541 tagcagacgt ggatgcaggc gcaaatttga aactcagctg caggaggttc atggagagca
     3601 ccgcattcag aaggccgagc accagcaggc gaaagaagtc tttttgcgtc agcttcacat
     3661 cgtgcttttt catgtaagac agagcgaagg gaaggagtac caagccgccc agaagaaacc
     3721 ggaaagcatt cacaaacatc gtcgtggtag agcttgtcag agtgtgcccc acaatttcgt
     3781 aggtgctaaa gagaaagatc gttagaagta gccacatata cccttgcact ttttttgtca
     3841 cgtccgatca cctttctgta gtattattaa gataaacttt tgtctcggga tttttgtagg
     3901 aattaagctt ttgtggaaga ataatgcaaa tggaggtgct tttgatgcgt tttgaaaatg
     3961 agggtggaaa agcaaccttt aatggtaagg agataaaaaa tccatttgtc agattgcttt
     4021 tcggcatcgt ctgcgtactg gtctcagctc ttattctatc agttattttg gccgtgctgc
     4081 tacctgtttt cggtagatca atcgatttga cggtgcttca ctacattgct cttgtccttg
     4141 gcgtcttggt gatcattccc accatccttg cgggaagtct ggtaacggga atcgtatccg
     4201 gctctataga gttgttttca tcgagcaagt ccaaagagaa aaagcaaaca gacgtaaaga
     4261 agaagaagta gcctggagtt tgcaggtttc agactctttc tgccaagaca ctaacaagtc
     4321 ccatgcgctt ttcagccatg ggtttttttt atggccaagc aggtgataaa cactacaaaa
     4381 ttagtacatc tgtcacagag acttctagca ctagatacct ctttcctacg taatgtatag
     4441 ggaaccagaa aagaggtgga tttcatgtct caaagcaaag aaacgaagta taccgtgaaa
     4501 agaggcgaca cccttaagtc aatcgcctta gaaaacaatc ttaccgtaga agccatctta
     4561 aacgccaatc cagatattgc cgaccccgaa gacctgagat caggtcaaag catagttctg
     4621 ccacttttaa acaccagtgg ctactcttct ggtgattatt acactgtcaa ggtgggagat
     4681 actttggaat ctatagcaaa ggaacacgga actgcggtcg atgccatcct gctgaagaac
     4741 cccaagctga ttgacacaga ctcagtagag gcaggagaaa ctatagagat tcccaaggct
     4801 cacgcatcgc agattcctcc tccgcctgca acctgcagcg gacgctatta tacggttgta
     4861 gcaggcgaca gcctttacct catcgctcaa agaaacaata taaccctgga tgcactgctt
     4921 gccgccaatc cccagatcac agatcctgcg cggatctcgg taggtcaagt gatctgcgtt
     4981 ccagggacta caccttcatg cgaaggatcc ctatacacta tcagagctgg agacactctg
     5041 tttagtctgg cgcaaagata caacactact gtagatgcaa ttattcgggc aaaccccggc
     5101 attgatccaa acaatttacg tataggtcag gta
//
LOCUS       DAOSDA010000064         5118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_10218_length_5118_cov_3.708078, whole genome shotgun sequence.
ACCESSION   DAOSDA010000064 DAOSDA010000000
VERSION     DAOSDA010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10218_length_5118_cov_3.708078"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLW82_02380"
     CDS             complement(<1..210)
                     /locus_tag="PLW82_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46152.1"
                     /translation="MGRIQFRICALRVSQKLETANKMNKGRLLSDQRREGGSRWRLFV
                     RISAEDTSFCPESRDAADPTGGDSVC"
     gene            complement(318..1643)
                     /locus_tag="PLW82_02385"
     CDS             complement(318..1643)
                     /locus_tag="PLW82_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46153.1"
                     /translation="MRKTAYLLIALMLLSVGVFAAAPEKVEQVVVNLILFDGSLWKNT
                     ISPQEYKTFYMMANADNILVPTNTKVYFWPITQEYMADFYDLNEEIEGTMVVSRGNRV
                     IARIEKEEYAFAYPNGYYTGSPQLVRGEEAHKASAKYKDEVNAYYQRVMEYQQALIDY
                     QVALEKFWENPEAYRGRESEIPKEPKIPEYPTYYATEVQRGYIVNLEPGTYTIEIEGM
                     PETKRTAIAFTPRRQGVGYDIRPEQQWTVPRPSNDRSETVYVYGKQTIYVTPYKGIEV
                     NHFMYEKLKKLPSELSGRGGELVYKWMALEYLNGGTLEVYKDGELISTSDFKQWYVDQ
                     TAGAALGYNIIEFDPKVQGERPPSFEGFRVQLEGSGRYSFRLVDKDGNVVQESERLIR
                     SIDSRSRFFAVILPIVPILIGGLIWVWRASLSATDTGTDKTSKLEVAKS"
     gene            complement(1640..2512)
                     /locus_tag="PLW82_02390"
     CDS             complement(1640..2512)
                     /locus_tag="PLW82_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ46154.1"
                     /translation="MNRFRAVAIRAIAGRELRSTLFNMGIYVAIALGLIVASFMIYTY
                     VTGIMEYGMAVMPSSKTETRYYSELPQSPEPMLVPLQWTVYIGAIYITLSASLSISRE
                     RDQGTLEVLFYGPVDSLSFVIAKFVEQLLSFGVVLLFYIAYFVVMSAISKFALGVGFL
                     KMIILSLFLAGCMASFGMFLSSMTRKVKTSVILFLVFVITFLAVPMIHQFVMVQYTST
                     MKGAVSVARHVTSTLNFLVSWISPFAYLGRGTTAIILGSTSELIKSLFFSLIYTVVFL
                     TASIVIFNRKGVRR"
     gene            complement(2509..3453)
                     /locus_tag="PLW82_02395"
     CDS             complement(2509..3453)
                     /locus_tag="PLW82_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46155.1"
                     /translation="MIKTHNLTKEFTTTVAVKDLELNVDNGEIYGFLGPNGAGKTTTI
                     MMLLGLEKPTAGSIEVFGKPLDKNYFEIKRKVGVVSENQYFYGEMTAWEYLNFFGRLY
                     EVDEREKKIKALLERVKLYHRRNEMLLGYSHGMKQKIAIARALLHDPDLLILDEPQNG
                     LDPYGIRDIRDIIGEENAKGRTVLISSHILSEIEQLCTRVGILSKGRLVAEGPLDIVK
                     TKVQPDTTLIVEIENLTDAIKDKLASEPYVKKMQVEENKLILSVDREGDYRAQVARII
                     NENSGLVVGMQTKTYSLEEAFIKITESSLESLIKEGTA"
     gene            complement(3605..4216)
                     /locus_tag="PLW82_02400"
     CDS             complement(3605..4216)
                     /locus_tag="PLW82_02400"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019393819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOJ46156.1"
                     /translation="MAANSTCRLWKVGAKLLIPLLILGSCLTIIIFTFDLDSRSSAML
                     LNSFEHSAPKDLERTEDESGAPAIELPHPYDLIIATATEYGVDPALVLAVADIESNYK
                     VDAMSHKGAIGVMQLMPATARELGVNPYILEENIEGGVRYLAKLLAKYGDVKLALAAY
                     NAGMGIVDRYGGVPPFRETQNYVKKVLSRYGEYSDLVRLALRS"
     gene            4508..>5118
                     /locus_tag="PLW82_02405"
     CDS             4508..>5118
                     /locus_tag="PLW82_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ46157.1"
                     /translation="MFFLIISLCIMLTIQTHAFIPERPAILIHGIGFKGGLTWDGIPL
                     LRYLNLQNRGMTDFLVESGGYTLGKDLFLYNYDTLDPIEHIASEFAVFLDKHLEETGT
                     EEVDLICFSMGGLIARTYLNGKENPPVRNLITIGTPHRGSYWVEVAEQVKPQSGSWFF
                     RTLEDFLAKNDGSGASLDNDIYSFLDTVYRLSDYPSLGQMDPEG"
BASE COUNT         1291 a         1276 c         1146 g         1405 t
ORIGIN      
        1 gcaaacacta tcacctcctg tgggatcggc ggcatctcgg ctttccgggc agaagctggt
       61 atcttctgcg cttattcgaa cgaacaatct ccagcgcgag ccaccttctc ttctttggtc
      121 tgaaagaagc cttcctttgt tcattttatt ggcggtttcc aatttttgac ttacccttaa
      181 ggcacagatt ctaaactgga tccttcccat gcatgtacct tctctagtag cagcgtccgg
      241 tttcagccaa ccaaaagcaa aacctactgc aaactaaaaa aggggcgttt gacagtgact
      301 gtcaagcgcc ctcttcgtta actttttgca acttctaact tcgatgtttt gtctgtacct
      361 gtatcggttg cggaaaggct tgcgcgccag acccagatca ggccgccgat caaaatagga
      421 acgatgggca gaatcaccgc aaagaagcgg ctgcgggaat caatagatct gattaaacgc
      481 tcgctctcct gcaccacgtt gccgtctttg tcgacgaggc ggaaggaata cctaccgctt
      541 ccctccagtt ggactctaaa gccttcgaaa gatggaggtc tttcgccttg cactttgggg
      601 tcgaactcga tgatgttgta gccaagtgca gcgccagctg tctggtccac ataccactgt
      661 ttgaagtcgg atgtggagat aagctctccg tccttataaa cctctaaagt tcctccgttt
      721 agatactcca aagccatcca cttgtacacc agctcgccgc ctctgccgct gagctcgcta
      781 ggcagcttct tcagcttctc gtacataaag tggttgacct cgatgccttt gtaaggagta
      841 acatagatcg tctgcttgcc gtagacgtag accgtttcac tgcgatcgtt gctgggtctt
      901 ggaaccgtcc actgctgctc aggtctgatg tcgtagccta ctccctggcg tcttggcgtg
      961 aacgctatcg ccgttctctt ggtttccggc ataccttcaa tctcaatggt ataagttccg
     1021 ggttcaaggt tgacgatgta tcctctctga acctctgttg catagtaggt tgggtactct
     1081 gggattttgg gttctttggg aatctcactc tcacggccac ggtaggcttc ggggttttcc
     1141 cagaacttct ccagagctac ttggtagtcg atcaaagcct gctggtactc cattaccctc
     1201 tggtaatagg cgtttacctc atccttgtac tttgctgaag ctttgtgagc ttcttcaccg
     1261 cgtacaagct gcgggcttcc agtgtagtag ccgttaggat aggcaaaagc atactcttcc
     1321 ttctcgattc tggcgatgac tctattacct ctagatacaa ccatagttcc ttcgatttct
     1381 tcgttcaggt cgtagaagtc ggccatgtac tcttgcgtga tcggccagaa gtagaccttg
     1441 gtgttggtag gaaccaagat gttgtctgcg ttggccatca tgtagaaggt tttgtactcc
     1501 tgcggagaaa tggtgttttt ccaaagtgag ccgtcgaaaa gaatcaggtt aacaactacc
     1561 tgctctactt tttcaggggc agctgcaaaa acgccaacag acaaaagcat tagcgctatt
     1621 aaaaggtatg cagtctttct catctccgca ccccttttct gttgaagatc acgatgctgg
     1681 ctgtgagaaa taccactgtg tagatcagcg agaaaaacag cgacttaata agctcagatg
     1741 tgctgccgag aatgatggct gttgtacccc gacccaaata ggcaaatggt gaaatccagg
     1801 ataccaagaa gttcagggtc gaagtaacgt gtctggctac ggaaacagca cctttcatgg
     1861 tgctcgtgta ctggaccata acaaactggt gaatcatggg aaccgcaagg aaggtaatga
     1921 caaacaccaa aaagagaatg accgaagtct taaccttgcg ggtcatgcta gaaaggaaca
     1981 taccaaacga agccatgcac cctgcaagaa acagcgacag gatgatcatc ttcaagaatc
     2041 ccacgccaag tgcgaacttg gagattgccg acatcacaac gaaataggcg atgtaaaaca
     2101 gcagcaccac gccgaaagac aaaagctgct caacgaactt ggcaatcacg aacgacaggc
     2161 tatctaccgg tccgtagaac aacacttcta gagttccctg atccctctca cgggaaatgg
     2221 acagggatgc ggacagggta atgtaaatag cgcctatata cacggtccac tgcaggggaa
     2281 ccagcatcgg ttctggagac tgtggcagct cgctgtagta gcgagtctcg gttttgctag
     2341 atggcataac tgccataccg tattccatga tacctgttac ataggtgtag atcataaacg
     2401 atgccactat aagtcccaaa gctattgcta cgtatattcc catgttaaag agggtggagc
     2461 gcagctcacg ccctgcaatg gcacgaatag ctactgcacg aaatctgttc atgctgtccc
     2521 ctccttaatc agtgattcta ggctgctttc tgtgattttg atgaaggctt cttccaagct
     2581 gtaggtcttg gtctgcatcc caaccacaag gccgctgttt tcattgatga tcctggcaac
     2641 ctgagctctg taatcgcctt cacggtccac tgaaaggatc agcttgtttt cttctacctg
     2701 cattttctta acgtagggct cggaggccag cttgtcttta atggcatctg tcaggttttc
     2761 gatttccaca attaatgtgg tatcgggctg taccttcgtt ttgacaatat ccaaaggccc
     2821 ttcagcaacc agtctgcctt tgctcagtat gccaactctc gtacacagct gctcaatctc
     2881 agacagaatg tgtgaagaga ttaagaccgt tctgcctttg gcgttttctt caccgatgat
     2941 atcgcgaata tccctaatgc cgtacggatc gagaccattt tgcggttcat caaggatcag
     3001 cagatcggga tcgtgcagca aggctctggc gatagcgatc ttctgcttca taccgtgaga
     3061 gtagcccagc agcatctcat tgcgtcgatg gtaaagctta actctttcga gcagagcttt
     3121 tatcttcttt tctctttcat ccacttcgta aagacgccca aagaaattca aatactccca
     3181 tgcggtcatt tccccataga agtactgatt ctctgagaca acaccaactt ttctcttgat
     3241 ttcaaagtaa tttttatcga gaggtttgcc aaaaacctca atagaccctg cggtgggctt
     3301 ttccagaccg agaagcatca taatagtggt agtttttcca gctccgtttg gccccaaaaa
     3361 cccgtagatc tcaccgttat caacattaag ttcgagatct ttaaccgcaa cggtcgtcgt
     3421 gaactctttc gtaaggttat gcgtctttat caagtgtcgt ccctcccatc catatcaatt
     3481 tggagccatc cccatctcta ttgctcttgt ttttgattca acaagtcgca atggtgttcc
     3541 tcttttatag acgtagaagg gttctcaaag tttgagaaaa aaaagaaaag ctagcttttt
     3601 tttgctagct tctcagtgca agtcttacca aatccgaata ctcaccgtat ctcgataaaa
     3661 ctttctttac atagttttgt gtctcgcgga aaggtggaac ccctccgtag cgatcgacta
     3721 ttcccatgcc tgcgttgtat gcagctagag ccagtttcac atcaccgtac tttgccaaca
     3781 gcttggcaag gtacctgact ccgccttcga tgttttcttc cagaatgtag ggatttaccc
     3841 ccaattccct agcggtagcc ggcataagct gcataactcc tatcgcgcct ttgtgagaca
     3901 tagcatccac cttataattg gactcgatat ctgcaactgc cagtactagt gccggatcga
     3961 caccgtattc tgtagctgtg gcaattataa ggtcgtatgg atggggcagc tcaattgcag
     4021 gtgcaccaga ctcatcctct gtgcgctcta aatcttttgg agcagaatgc tcaaacgaat
     4081 taagtaacat agctgaagat ctagagtcca aatcaaaagt aaagatgata atcgttaggc
     4141 acgagcccaa gattagtaga gggatgagta gcttggctcc gaccttccaa aggcggcagg
     4201 tactgtttgc tgccaagttt atgacctcct tatgggaaaa acccatatat gtcaatgttg
     4261 atctagcgaa ctggtaaata tgggtacttc gacttacttg taagattgta cttgactaaa
     4321 ctttttctgt caagtattgc ctcttcttac aatttttctg aaataaacct gtaaatgtaa
     4381 tacctccaca gggaaaaact aaacatctca atcagactgt aggtcccgct tccttgtcgc
     4441 acccacaagg gtgcctgtat tgacgggcta cttctatgct attagacttg acttaagagg
     4501 tgatagcatg ttcttcctaa ttatttcgct ctgcattatg ctaacgatac agactcatgc
     4561 gttcatcccg gaaagaccgg ccatcctcat tcacggcatc gggtttaaag gcggtctcac
     4621 ctgggatggt atacctcttc tgcggtattt aaacctccaa aacagaggta tgaccgactt
     4681 tctagtagag tcaggaggat atactcttgg aaaggatctc ttcctctaca actacgacac
     4741 ccttgatcct atcgaacaca tagctagcga gtttgccgta tttctcgata aacatctgga
     4801 agaaacgggc acggaagaag tggatctcat ctgcttcagc atgggcggtc ttattgcaag
     4861 gacatatctc aacggcaagg aaaaccctcc tgtaagaaat ctgatcacca tcggcacgcc
     4921 ccaccgtggg tcgtactggg tggaagttgc cgagcaggtg aagccccagt cgggcagctg
     4981 gttttttcga acacttgagg attttcttgc aaaaaacgat ggatcgggcg catctttaga
     5041 caatgacatc tacagctttc tcgatacagt ttaccggctc agcgactatc cttctctggg
     5101 acagatggat ccagaagg
//
LOCUS       DAOSDA010000065         5072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_10362_length_5072_cov_3.305561, whole genome shotgun sequence.
ACCESSION   DAOSDA010000065 DAOSDA010000000
VERSION     DAOSDA010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10362_length_5072_cov_3.305561"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3057)
                     /locus_tag="PLW82_02410"
     CDS             complement(<1..3057)
                     /locus_tag="PLW82_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46158.1"
                     /translation="MALKTPTKYVHPAVKAVFITLGIVLALLILVNAGLIVAFNQSSI
                     SDTLRWQIITTLEELTGRHVDIGAIGPDIFNRIVIEDLVIGPSLQEGIDEGLKDTNQD
                     FFTVGRITIEYNLLELIKNNFDYLKAVEAVVLYKPTVSFEFVEELGRWNFDDLFSRSQ
                     GPVSFSIPEGLPVSATQGTAKVLGFPLFSRDEDIEGRKLALNAEVVSGNKIRIEIDGE
                     VSFPVEGWRASQWPDQWLPQGKTHTAFYPVRGTGEIDLTNGTWSVKLTATNVELTDFN
                     YWLPPNLGVDIHRGKGDLELNLVFNQGFVDFSGILQVKNAGLNYFLVPFAIERASGTI
                     RFDSQGIQSIEAVGYGGDTTVTATGYTAGGWNDPRVYVEMHTEKGNLKPFAGYLKEGF
                     YRQIAKIKDGELPPVAQYELPASKPSLLWLEEFSVDGFFDGELVLSGYLSQLVFEARA
                     KIYETTIEHPQLYLPLKNVSGFLVYEDGNVTIDSFTGNLDSGIVNIAGSVEDFFGRPY
                     LNLKVGAKNLNIKQPAESYLNLKVEGLAGLQMDLAGFLDQSFALFEATISPGSVEGFN
                     HEGFTAAGRVANGILEIDDLKGSIMGSSVLATGALILPEDIASVLYPHQTLKGDSFLE
                     VNIQGLAPREAANALPALLEKLPIDPSRIEGEVDISLLARVSDWNLDEADVVGNVESR
                     SLAYDDIRAFNISGGFFMKKDHIGIESIQAEIAPATVLVSGTVPLVTGRDLDLNLKVN
                     TFEPASLAALYPILNDVGGRIDLNLQVGGSLEAPLLTGSVDWLNPSFREVRLKRIFGT
                     MKGNPDLEVIDLTSMSVETLSGKTHSVSGHINTKNQLSAGLRVSVKDQEISEFLEPFD
                     LEQYASGPVSFDLTLDGTFDNPFLKLELYQGPSNIYGFAARDINLTASYQNGLVLITR
                     ARGQVDEGSFSIFGTADLNKLSADLQINVERFPVAALPIPDEYREYVAGGLLSYRGKA
                     SGTLASPEIRGEAVIRDGVFLGERVDRLGVLFEIKGGDLSITNSEIK"
     gene            complement(3103..4770)
                     /locus_tag="PLW82_02415"
     CDS             complement(3103..4770)
                     /locus_tag="PLW82_02415"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HOJ46159.1"
                     /translation="MLITITIFGANFPLEDVKPGLTGVGKTVFYGTEIEEFPVEIIGV
                     IPGEGLVENYIMIKVGGKALELGGVSEGMSGSPVYIDGLLLGAISHGFDDIGHAYGLV
                     TPIEYMEQILTISRGIKPEIRKIDSITAKPLSGRLFASGFGRRSQEFLSKQLGTPLYI
                     TVGPFSGTNPFIPQDMAPGSALGVQLVSGDVSVGSYGTLTKIYDDGRFLGFGHTILNR
                     GSTSFLATSVFVHATLQGQPTSYKLASLTGPVGTITEDRGAGILGSFGKMPNMVPVRV
                     VVRDEDLGRTVVTRAEVIPDNTLFGRLAASTVLEGFDRGLDRVGAGTSKVRFTVKGEG
                     LPYPVVRTNYFYTYSDIAALSLNEIAEVLDLLLYNDYYDIKISEVFVEADFTEEKHTA
                     VIVSATPTKKQVYPGEELQIEVQIRPFRGEYQTLNVVLPIPEDLPAGRVQVMVRPGMS
                     IAIYGEPAPSVTEKLELQSEERVGLPVSQEALDWEKVLQEFMNREKNNQIVVEFYPPY
                     SDIISDEISTDEALSPIQVRLDTEYVMEGDAAWELEILEKPKDSQAD"
BASE COUNT         1267 a         1342 c         1163 g         1300 t
ORIGIN      
        1 cttgatttcg gagttggtta tagacaggtc ccctccctta atctcaaaga gaacacctag
       61 tcggtcgaca cgttcgccca ggaagacgcc gtctctaatc accgcttctc ccctgatctc
      121 cggcgaagct aaagtaccgc tggctttccc tctgtagctt aaaagtccgc ccgccacata
      181 ctccctgtac tcatcaggaa tcggcagggc agctacaggg aatctctcaa cattgatctg
      241 caggtccgcg ctcagcttgt ttagatcggc agtgccgaag atggaaaagc tgccttcatc
      301 gacctgccct cttgctctgg tgatgagcac cagtccattt tggtagctag cagtaaggtt
      361 aatgtccctt gctgcaaagc cgtagatgtt cgatgggcct tggtaaagtt cgagcttgag
      421 gaaggggttg tcaaaagtcc cgtccaaggt aaggtcgaat gacaccggac cagatgcata
      481 ctgctccaga tcaaagggtt ccaagaactc tgaaatttcc tggtccttga cgctgacccg
      541 tagacctgca ctgagctggt ttttagtgtt gatatggcca gagacgctgt gggtcttccc
      601 gcttaaagtc tccaccgaca tactggttaa atctataacc tcaaggtcag ggttgccctt
      661 catagttccg aagatgcgct tcaagcgcac ttcccggaag ctcggattca gccaatctac
      721 acttccggtg agaagaggag cttctaaaga tcctcccacc tgaagattga ggtcgattct
      781 tccgcctaca tcgttcagaa tggggtatag agctgccaaa gaagcgggct caaaagtgtt
      841 taccttaagg ttcaaatcca gatcgcgccc tgtcaccagc gggaccgtac ccgagactag
      901 gacggtagca ggtgcgattt ctgcttgaat gctttcaatg ccgatgtggt ccttcttcat
      961 gaaaaatccg ccgctgatgt tgaatgcccg gatgtcgtca taggccaggc ttctggactc
     1021 tacattgccc actacatcag cttcgtcgag attccaatct gagacccgag caaggagcga
     1081 aatgtccact tccccttcga tcctcgaagg atcgatagga agcttttcta aaagcgctgg
     1141 cagcgcattt gcagcttctc tcggagccag accttgaata ttgacctcaa ggaagctgtc
     1201 tcctttcagg gtctggtgcg gatagaggac actagcgata tcttcaggaa ggatcagagc
     1261 gcctgtcgcc aggacgcttg aacccataat gctgcccttg aggtcgtcga tctccaaaat
     1321 accgtttgca acgcggcctg ctgcagtaaa gccttcgtgg ttaaagccct ctacagaccc
     1381 gggagatatc gtggcttcaa agagcgcaaa cgattgatct aagaatccag caagatccat
     1441 ttgcaaacct gcaagacctt cgactttcag gttgaggtaa ctttcagccg gttgtttgat
     1501 gttcaggttt ttggcaccaa cctttaggtt gaggtatggc ctgccgaaga aatcctccac
     1561 acttccggcg atattcacta ttccactgtc aaggttcccg gtaaacgagt cgattgtgac
     1621 attaccatct tcatagacca aaaaaccact tacattcttt agtggtaaat acagctgagg
     1681 atgttcaatt gtggtttcat agatcttggc tctggcctca aacaccagct gggaaaggta
     1741 tcccgaaagg accagttcac catcgaagaa cccgtctacc gaaaactcct ccaaccacag
     1801 gagggatggc tttgaagccg gaagttcgta ttgagcaaca ggcggaagtt cgccgtcttt
     1861 gatcttggcg atctgcctgt agaagccttc tttcagatag cccgcaaagg gtttgaggtt
     1921 ccccttttcg gtgtgcatct ctacgtacac gcgggggtca ttccagccgc ctgctgtata
     1981 accggtggca gtaacggtag tgtcccctcc gtatcccaca gcttcaatgc tttggattcc
     2041 ttgactgtcg aaacgtatgg tccccgatgc tcgctctatg gcaaaaggca ctaagaaata
     2101 gtttagccct gcatttttta cctgcaaaat gccgctaaag tccacaaatc cctggttgaa
     2161 gaccaggttc agttccaggt cacccttacc tctgtgaata tccacgccga ggtttggtgg
     2221 aagccaatag ttgaaatcag tcagttccac attggtggct gtcagcttga cactccaagt
     2281 tccatttgtt aaatcaatct cacctgtgcc tcttacaggg taaaacgccg tgtgggtttt
     2341 gccttgcgga agccactgat cgggccattg ggacgctctc cacccttcca cagggaagga
     2401 gacctcccca tcgatttcaa tacggatttt gttccctgaa acgacttctg cgtttaaagc
     2461 caacttgcgg ccttcaatgt cctcatcacg ggaaaacagg ggaaatccca aaactttcgc
     2521 agttccctga gttgccgaaa ccggcaaacc ctcgggaatg gaaaaagata caggtccttg
     2581 tgacctgctg aatagatcgt caaagttcca cctacccagc tcctcaacaa actcaaagct
     2641 gacggtgggt ttgtaaagga ctacggcctc tactgctttg agataatcga agttgttttt
     2701 gataagttct aaaagattgt attcgatggt gattctgcct acggtgaaaa agtcttggtt
     2761 tgtatcttta agaccttcat ctataccttc ctgcagcgaa ggacctatca ccaggtcctc
     2821 gatgacaatg cggttaaaga tgtccggacc gattgcacca atatccacat ggcgacctgt
     2881 aagctcttcc aaagtggtga taatctgcca ccttaaggtg tcgctgattg agctctgatt
     2941 gaacgctaca atcagcccgg cgttgaccag aatcagcaaa gccagaacaa tacctaaagt
     3001 gatgaacacg gccttgaccg caggatgaac gtatttagtt ggagttttta gtgccatgtc
     3061 ctaccggctg cgaccggatt cacctcctta agtgcgaggg agctaatcag cctgggaatc
     3121 cttcggtttt tccagaatct ccagttccca agctgcatcc ccttccataa catactcggt
     3181 atccagcctt acttgaatag gcgaaagagc ctcatctgta gagatctcgt cgctgatgat
     3241 atcgctgtat ggagggtaaa actctaccac tatttgatta ttcttctctc tattcataaa
     3301 ctcttgaagt actttttccc agtccaaagc ttcctggctt acagggagcc ctacgcgttc
     3361 ttctgactgc aattccagct tttcggttac cgaaggcgcc ggttctccgt agatggcaat
     3421 agacatcccc gggcgaacca taacttggac tcttcctgca ggcaggtctt ccggtatcgg
     3481 gagcaccaca ttgagagtct ggtactcgcc tctgaacggt cgaatctgaa cttcaatctg
     3541 cagctcttcg ccgggatata cctgcttctt ggtaggagtt gcgctgacga ttactgcagt
     3601 atgcttctcc tcggtaaaat ctgcttccac aaagacctcg gagatcttga tgtcatagta
     3661 gtcattgtag agcaggagat ctagcacctc tgcaatttcg ttcaaactta gggctgcaat
     3721 atcagagtag gtgtagaagt agttggtgcg taccaccgga tagggaagcc cttcaccttt
     3781 tacggtgaac cgcactttag atgtgccggc tcccactctg tcgagacctc ggtcgaagcc
     3841 ttcgaggacc gtgcttgcag ccagccgccc gaagagggtg ttgtcgggga tgacttccgc
     3901 tctggtgacc acagttctac ctagatcttc atctctgacc acaactcgca caggcaccat
     3961 gttcggcatc ttcccaaacg atcccaagat gcctgctccg cggtcttcag tgatggtgcc
     4021 tacaggaccg gttaaactgg caagcttgta tgaggttggc tgcccctgca aagttgcgtg
     4081 aacgaaaact gatgtagcca ggaaagacgt gcttccccgg ttcaaaatag tatggccgaa
     4141 gcctaaaaat cggccatcgt cgtagatttt tgttagggtt ccgtagctgc ccacagagac
     4201 gtcaccagac accagctgca cgcctaaagc cgatccggga gccatgtcct gaggtataaa
     4261 aggattagtg ccactgaagg gtccaacggt aatgtacagc ggtgtgccta gctgctttga
     4321 aagaaactcc tggcttctcc ttccgaaacc gctggcaaaa agacgcccgc ttaagggctt
     4381 tgcagttatg gagtcgatct tccgtatctc cggcttgatg ccccgagata tcgtcagaat
     4441 ctgttccata tactcaattg gggtaacaag accgtaagca tggccgatat catcgaaacc
     4501 gtgactgata gcgccaagga gcaaaccgtc gatatacacc gggctcccgc tcattccttc
     4561 agacacgcca ccaagctcta gggctttacc tccaacttta atcataatat agttttccac
     4621 caaaccttca ccgggaataa ctccgatgat ctccacagga aactcctcga tttctgtgcc
     4681 gtagaaaacc gtctttccta caccggtaag accgggctta acatcctcaa gaggaaaatt
     4741 cgccccaaaa atcgtgattg ttataagcaa aaataaaaac agtactctcc atcttttcaa
     4801 ttcaaacacc tcatttacca aaaagcattg gacaaatctt tcctctttct actatcaaac
     4861 aagccagcct agtaactcaa cgctacatga acaatgagaa ctggccaaaa accctgttta
     4921 cagcgaaaaa taccagtcta gttaatactt gtgtatttct tttactagta ttccctttcc
     4981 tcctttactg gcaaacaaac atctttccag aacagcgaaa aaggggctgt tgcacaatta
     5041 gccgattaag ctgattgtgc ggcagccttt tt
//
LOCUS       DAOSDA010000066         5019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_10521_length_5019_cov_3.268533, whole genome shotgun sequence.
ACCESSION   DAOSDA010000066 DAOSDA010000000
VERSION     DAOSDA010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10521_length_5019_cov_3.268533"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLW82_02420"
     CDS             complement(<1..488)
                     /locus_tag="PLW82_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOJ46160.1"
                     /translation="MNSKDFIIVTGLSGAGKSLAADVLEDLGYFCVDNLPPLLIPKFA
                     ELVAGSSSEIQKAALVSDVRGGRFFDHLLQALESLERMGIKYEILYLEASDEVLIKRY
                     KESRRRHPLASAIESGSIVQSLELERAKLWSIKERASKVIDTSALSTSGFKEVLSNWL
                     EK"
     gene            complement(534..1469)
                     /locus_tag="PLW82_02425"
     CDS             complement(534..1469)
                     /locus_tag="PLW82_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOJ46161.1"
                     /translation="MKKTGYIAIILMIIVLVAPVHALKRVWSSDRLLYDAVLGVETVK
                     GSPDRLLVWGKNYERAEAKVALVEIKNQKINVIWESQNFYERGSNLMCAVGSFSSADQ
                     EVIVLAENLWKLYKIESGGLKEIASGTGITGVMEVTSGDVDGDGRTELIMTGVHELLR
                     DTVDKKIVVYKYQNGKLAKVYETKGLGNIRALTSFDFTGKGKADIVAEVGLGYKQGEI
                     TVLQDYKQIVTSGLRPQPVFALSHVDGRLIVGDDSGVVTIYRLVTGSTPRFEQVDSAS
                     SVGWGLVDVASGDFLGRKAKQIAVISSPTGITIFE"
     gene            1667..2440
                     /locus_tag="PLW82_02430"
     CDS             1667..2440
                     /locus_tag="PLW82_02430"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOJ46162.1"
                     /translation="MYSNSAQRPDQAKAIHPLIYKGPESELPSGYNKDCLIAMPRDPY
                     WLFFYWDFAEQTWQKIAEYKNHVCLLKISSEGTEITSVPVSISAKSWYVNANSPGLSL
                     TAHLGFETDNGFLPILTSNTVCMPRDSISSKTDELWLFIDELYRSEYFGTIGGSPLFW
                     QVEGNQKAQYAASPMPPAKLKEGEDLHHYLVVDTDLIVYGKTTPGSALIQNGQAVPVD
                     CNGNFEIRLKLCEGLHEIELVSFAQNGQVKTKNITVKRG"
     gene            2445..4016
                     /locus_tag="PLW82_02435"
     CDS             2445..4016
                     /locus_tag="PLW82_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOJ46163.1"
                     /translation="MAKGYVAIILHAHLPYVRDLSPDGALEEDWLYEAIIETYVPLIK
                     VLERLEAERLPVRLGVSLTPPLLNMLSDSVLMQRFKKRLDVLIEMAAEEVKRTKNSYP
                     FTETAKLYQKILTDTKTRIDSSENYIISRFAHFQDVGLVEILASCATHGYLPLMDVVP
                     ESQRAQIKQGVREYRRFFGKDPLGFWLPECGFTPGVDKYLADESLRYFLTDTHGVLKA
                     DTRPRYANFAPIYTTNAVAAFGRDIESSKQVWSATEGYPGDFDYRDFYRDIGYDLDYE
                     YLKPLLEPIGIRKMTGFKYYRITGKSDYKEPYVPEHARYKARLHAANFVFNREAQVRY
                     LSSVMDRPPIIVAPYDAELFGHWWFEGPIFLEEVLRLTCKSPTLELVTPSDYLKKHPT
                     NQVATPSSSSWGYNGYNEVWLERSNDWIYPHLHEASTRLNLLAKKNEPNLLKTRILNQ
                     AARELLLAQSSDWAFIMKTGTVSQYAVKRTKEHLRNFHDLLGLCDGAPVSLDKLQQLE
                     ERNAIFPEIDYRIFA"
     gene            complement(4127..>5019)
                     /locus_tag="PLW82_02440"
     CDS             complement(4127..>5019)
                     /locus_tag="PLW82_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46164.1"
                     /translation="PSFRLELKGAAVDGGFYAFSEGEYVTIRFFDAEGCYLDTARQRK
                     IENLLRSGDSLNPDTEPGKIYYNPSQFTIYLNRYQDFVVPKDTVCDSPAGRILLDQIK
                     SKEPVGLKAQISASGEHCRIWDEKGHELSQAELEAASVYLSLKRSPKNIKVPLGATSS
                     LEEIASEFGGHIERTRPGLCYLDESVIPWHDAFRFLTLICTNRDEPLYRILEKLPKKT
                     QIVRELSCPLEERSKAMLKFASSYGTSSQNEAGLYYKDDRSFLYLKPDERKPVLRLTV
                     EGESMELAEELVGRILDLIK"
BASE COUNT         1309 a         1339 c         1108 g         1263 t
ORIGIN      
        1 tctttttcca accagttcga aagaacttcc ttaaaaccgg atgtgctaag agctgatgtg
       61 tcgatgactt tgcttgctct ttcctttatg ctccacagct ttgctctttc gagttctaag
      121 ctttgcacaa tgcttccgct ttcaatggcg cttgccagag gatggcgccg tctgctctct
      181 ttgtatcgct tgatcaaaac ttcatctgag gcttccaaat acagtatttc gtacttaatc
      241 cccattcttt ccaggctctc caatgcctga agcagatggt caaagaaccg gccgcctcta
      301 acgtcactga caagagcagc cttttgtatt tcactgcttg aacctgcgac cagctcggca
      361 aacttcggga ttaaaagagg gggaaggttg tccacgcaaa agtagcccag gtcttcaagc
      421 acgtctgcag ctagagactt cccggctcct gaaagccccg tcacaatgat aaagtctttg
      481 ctgttcaaac cggttccctc ctacgcacaa tcccccagaa cttaccctca agtctactcg
      541 aagatagtaa tgccggtcgg agaactgatc actgcaatct gcttggcttt tcggcctaaa
      601 aagtctccgc tggcaacatc cacaagaccc cagcctacag agcttgccga gtctacctgc
      661 tcaaaacgcg gggtgctgcc tgtcactagc cggtaaatgg tgacgacgcc gctgtcgtcg
      721 cccacgatga gccttccgtc tacatggcta agggcaaaaa ccggctgcgg tctgaggccg
      781 gacgttacga tctgcttgta gtcctgcaaa acggttattt caccctgctt gtagcccagc
      841 cccacctcgg caactatgtc cgctttgcct ttgcctgtaa agtcaaagga cgtcaatgcc
      901 ctgatgttgc caagaccttt ggtttcgtag actttagcca gtttcccgtt ctggtactta
      961 tagaccacaa tcttcttatc tactgtgtca cgtaaaagtt catgaacacc tgtcataatc
     1021 agttcggttc tgccatcacc gtctacgtca ccggaggtca cttccatgac accggtgata
     1081 ccggtaccag aggctatttc tttcagccca ccggactcga tcttgtagag cttccacagg
     1141 ttttccgcaa gaacgatcac ttcctggtcc gctgaggaaa aactgccaac tgcgcacatc
     1201 aaattagagc ctctctcata gaagttttgg ctctcccaaa tgacgtttat tttttggttc
     1261 ttaatctcaa ctaaagctac tttagcttct gctcgttcgt agttttttcc ccatactaac
     1321 agtctgtcag gggatccttt aacggtctct actccgagca cagcatcgta cagcaaccgg
     1381 tcggaagacc agaccctctt caaagcgtgt acaggtgcaa ctagtacaat aatcataaga
     1441 atgatagcga tgtaaccggt ctttttcatt ttactaacct caagaaccac cgttttcaat
     1501 cctttcaacc acaaatgcta tgaataagca gtacttttca cattcttcct tgcatgatgt
     1561 ttttcctgca ccgagcggca ccgtcaagca taaatcacct acccttagac tcataataga
     1621 ttagagatgc gatgcaaata cgacctagcg taaaggaggc agggctgtgt attccaattc
     1681 ggcacaaaga cctgatcaag ccaaagctat tcatcccctt atatacaaag gccccgaatc
     1741 tgaactgcct tcagggtaca acaaagactg tttgatagcg atgcccaggg acccctattg
     1801 gttattcttt tactgggatt ttgctgaaca aacatggcaa aaaatagcag aatataaaaa
     1861 tcatgtttgt ctgctcaaga taagctccga aggcacagag ataacaagtg tccctgtttc
     1921 gatttcagct aaaagttggt acgttaatgc aaattctcca ggtttatctc tcacagcaca
     1981 cctggggttt gaaacggaca atggttttct gccgatcttg acgtcgaaca ctgtctgcat
     2041 gcctagagat tcaatctcga gcaaaaccga cgaactctgg ctttttatcg acgaactcta
     2101 ccgcagcgag tacttcggaa caatcggcgg atcaccgctt ttttggcagg ttgagggcaa
     2161 tcagaaagct cagtatgctg caagtccaat gcctccggca aagctcaaag aaggagaaga
     2221 cctgcaccac tatcttgtgg tagacaccga cctcatagtt tacgggaaaa ccactccagg
     2281 ttcggcactg atccaaaacg gacaggcagt acctgtggac tgcaacggga acttcgaaat
     2341 acgcttgaag ttatgcgaag ggctccacga aatagagctt gtttcttttg cccaaaacgg
     2401 acaggtcaaa accaaaaaca ttacggtaaa aagaggttga taaagtggct aaaggctatg
     2461 tggctattat actacacgcc catctcccct atgttaggga cttaagtccc gatggcgcac
     2521 ttgaagaaga ctggctctat gaggcgatca tcgagacata cgtaccgctg ataaaggtcc
     2581 tggaaagact agaagcagaa agactccctg taaggttggg agtatcgctc acaccacccc
     2641 ttttgaatat gctttccgac tctgtactta tgcaacgctt caaaaagagg ctggatgtgc
     2701 tcattgaaat ggcagctgaa gaggtaaaga ggaccaagaa ctcctatcct ttcactgaaa
     2761 ctgccaaatt gtaccaaaaa atcctgacag acactaaaac ccggatcgac agttcggaaa
     2821 actacatcat ttcccgcttt gcccactttc aagacgttgg actggtggaa atccttgctt
     2881 cctgcgcaac tcacggttac ctgcccctaa tggacgttgt acccgagtct cagagggctc
     2941 agatcaagca aggcgtgcgt gagtaccgca ggttcttcgg caaagaccca ctagggtttt
     3001 ggcttccaga gtgtggcttt acccccggcg tcgataagta cctggcagat gagagtctcc
     3061 gctactttct caccgacacc catggcgtcc tcaaagcaga cacaaggcct cgctatgcga
     3121 acttcgcacc gatctacacc actaatgccg tggctgcatt cggcagggat atcgagtcgt
     3181 ccaagcaggt ctggtctgcc accgaaggtt accccggcga cttcgattac agggacttct
     3241 accgggatat cggatacgat ttggattacg aatacctgaa acctctttta gagccaatcg
     3301 gtatcaggaa gatgaccggt ttcaaatact accggattac cggaaaatcc gactacaaag
     3361 agccttatgt ccctgagcat gccagataca aagcaagact ccacgcggca aacttcgtgt
     3421 tcaacaggga agctcaggtt agatatctca gttctgtaat ggacagaccg cccattatcg
     3481 ttgctcctta tgacgcagag ctgttcggtc actggtggtt tgaaggccct atcttcctgg
     3541 aagaagtact cagactcacg tgtaagtcac ccacccttga actggtcact ccttctgact
     3601 accttaaaaa gcatcctaca aaccaagtgg ccactccttc ctcttcgagc tgggggtaca
     3661 acggttacaa cgaggtctgg ctggaacgct cgaacgattg gatctatcct catctgcacg
     3721 aagccagcac gaggttgaac cttctggcaa aaaagaatga gcccaacctt ttaaaaacga
     3781 gaattctcaa ccaagcagcc agagaactcc tccttgctca aagctcagat tgggctttta
     3841 ttatgaagac cggtaccgta tcccagtatg cagtgaagcg aacgaaagaa cacctgagaa
     3901 acttccacga cctgcttggt ctctgcgatg gagcccctgt tagcctcgac aaactacaac
     3961 agttggagga acgaaacgcc atcttcccag agatcgacta ccggattttt gcctaaagca
     4021 aacgccactc cccgttccct gtagcagcct tgagcaagcc agagaacaac tgcaaactca
     4081 aatagtaaag tgacgagaaa gctgttgcac cacgtgcaac agctttctat ttaataagat
     4141 ctagtattct tcccaccagt tcctcggcga gttccatgct ttctccttca acagtgaggc
     4201 ggagcacagg ctttctctcg tcgggtttta ggtagagaaa cgacctgtca tctttgtagt
     4261 ataggccggc ttcgttttgg gaactggtgc cgtagctgga tgcaaacttc agcattgctt
     4321 tactgcgttc ctccaggggg cagctcaact cccgcacaat ctgcgtcttt ttcggtagct
     4381 tctccaaaat cctgtaaaga ggctcgtctc tgtttgtaca gatcaacgtg agaaacctaa
     4441 aagcatcgtg ccacggaatg accgattcat ccaagtagca gagtcccggt cgtgtacgct
     4501 cgatatgccc cccaaactcc gacgcgattt cttccaagct gctagttgcg cccaaaggca
     4561 ccttgatgtt cttaggagaa cgctttaaag atagataaac gcttgcagct tcaagctctg
     4621 cttgcgagag ttcgtggcct ttttcgtccc aaatcctgca gtgttctcct gacgcagaga
     4681 tctgtgcttt caaaccgaca ggttctttgg acttgatctg gtccagaaga attctccctg
     4741 ccggagaatc gcagaccgtg tccttgggta cgacaaaatc ctgatagcgg tttaggtaga
     4801 tggtaaactg gctgggattg taatagatct tccccggctc ggtatcgggg tttaagctgt
     4861 ctccggacct caataggttc tcgatcttcc gctggcgtgc agtatcaaga tagcaccctt
     4921 cagcatcaaa gaagcggatg gtgacatact cgccttcgct gaaagcataa aaccctccgt
     4981 caacagcggc gcctttcagc tcaagccgaa aagatggta
//
LOCUS       DAOSDA010000067         4965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_10683_length_4965_cov_3.369654, whole genome shotgun sequence.
ACCESSION   DAOSDA010000067 DAOSDA010000000
VERSION     DAOSDA010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10683_length_4965_cov_3.369654"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..2005
                     /locus_tag="PLW82_02445"
     CDS             380..2005
                     /locus_tag="PLW82_02445"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ46165.1"
                     /translation="MLNNRGLKLLLVVLILSTSLCASTISVDQYKDKVLGGWLGQMIG
                     NIFGLPTEGHFITAMGPTNLTYYQSIPNSAFADDDTFNDLVWLFALEKYGLDLTSLDL
                     AEEWKIQLPAGSMACANWKARLNIEEGIMPPLSGHPSYNEYNLYIDAQIEADIWGLIS
                     PGMPDVGTDYASLMGELMAYSDGEYGGRFIAALYSLAFIEDDIPTMINKALEFIPKES
                     RYYEVIQAAIKAWETYPDSYRRARSEVYRNYYKAGQTPSSGWVIADVNGAMVVLALLY
                     GQGDFDQTLALAVQFGLDNDCNAATAGGILGAFIGAENIPDRWKNPLNNLYYNNVLRQ
                     APSYLLISDIADRTAKIGLANVEKRGGTVDLENNTITLPAYKVVSPEYNEPWSDDKVA
                     GNMLRGWEDGWEMANLGWDMSPGIRPVYEGRTNVFSSHPLDQKSPFYFYRTETLPADK
                     ITVLKTGVTSFNGDAHPTHFEADWELRVYVNDELIFSKIIERIDKKIVWYDLEVSLAE
                     YAGQTVTIRVENYPNNWSFEAGYWDYLRLEQID"
     gene            complement(2115..4289)
                     /locus_tag="PLW82_02450"
     CDS             complement(2115..4289)
                     /locus_tag="PLW82_02450"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ46166.1"
                     /translation="MPKLVVYYPPYPALEFKPDYIPRTDKDTITVEGQTDSDCLVWVG
                     EKPADVFPDGTFRAVVSLEEGENIICIKAQSSKTGEAVILQRRIIRDTVPPLLEIFEP
                     KDGAFVNTGRVTVSGKTEPKSRLFINGAVHPLLEDGSFRTEVSLTEGENFIYVAVFDE
                     KLPNMEMQVIQVNLDTVAPFLDIEFPDIDYIATEDYITVRGSTEPFATVAVTQHERKV
                     QADKDGKFSLRLPLDEGRNYFYFDVQDLAGNEISERVRIEKDTTKPQVREYKPTGILA
                     YSPGDILNLTLTASEARCEVTFSIGDKGPFPMTEVTSGNYSGVYRFQSDDIGEDMYIE
                     VTLTDKAKNTTTRRLTRVTVYDPAKPVVAQILAPNNPVSLYSGPSTEYDRVGMIKTIA
                     KVEVTARVGDYYRVSPAKTYAAWIHQSNLRFLEEGTAPSKPVISNITASKEGDWTNVT
                     FTMSEPAFYTITPLVHEPALLVTFYNTTSGLYHMAWRKDADFVKLITPMQITDDILQY
                     RIDLKGNTIYGYDVSVAGNNLNLRIKSKFTPFLEGKRITIDPGHGPDSGAVGPTGLLE
                     RDLNLEIALKLKELLEDAGCRVYLTRDKDYNYAPELSDRVAFAERYQTDLFVSIHNNA
                     ASNRSASGTESYYYTPYSHELARLSIKYMNEHQGSEYRFFAHRSFAVIRQWTMPAILT
                     EGDFVSNYSIEVWQKSGDFSTKNAEAIFMAIKEFVETFGFLD"
     gene            complement(4509..>4965)
                     /locus_tag="PLW82_02455"
     CDS             complement(4509..>4965)
                     /locus_tag="PLW82_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ46167.1"
                     /translation="KEKGCKVILDPAPAAKLSNEVLQMVDIITPNSTELQTLTGLPVD
                     TDEEAIVACRKLKEATSATVIMTRGSNGGLVIDGQGERFFPAYKVDAVDTTAAGDCFN
                     GSLAAALARGLSLDEAIDLASRASAVSVTREGAQPSLPTIDEVKAFFGL"
BASE COUNT         1225 a         1234 c         1172 g         1334 t
ORIGIN      
        1 atttctttac aaggaattcc agaacaggtc acctccccca agtttcaaac cgtcaaatgt
       61 taagccttat aacttcctta tgcgagaatg agaatccttg caaagtgctt tttataattg
      121 tatcaaaaac atggctccct tcaagcaaaa ttattatttt ttcaagctcg cagtgttagc
      181 tgtggcttgc ttcttgtatc ctcgttcgaa gccctcacct cttgttaaat ctttgacgac
      241 caactaccac tcggtgtcta tctggtaatt tatactgcag atcattccgt ttgtaagctc
      301 cgtttacttc aaaggaaatc aggagttttt tgtggaaaga gtttgaggaa acgcctactt
      361 aaactatagg gggtatatca tgctaaacaa taggggtttg aaactactac tagttgtcct
      421 aatcttaagt accagcttat gcgcctcgac catctcggtt gaccagtaca aagacaaagt
      481 tctaggcggc tggcttggac agatgattgg caacatcttc ggtctgccta ctgaaggtca
      541 ctttattacc gccatgggac ctacaaatct tacctactac cagtccattc cgaattctgc
      601 gtttgcagat gacgatactt ttaacgactt ggtatggttg tttgctttag aaaagtatgg
      661 tcttgatctt accagcttag accttgcaga agagtggaag atccagcttc ctgcaggttc
      721 aatggcctgc gccaactgga aagctaggct taacatagaa gagggcatca tgcctcctct
      781 gtcaggccat ccgtcctaca acgagtacaa cctctacatc gacgcccaaa tcgaagccga
      841 tatctggggt ttgatttccc cgggcatgcc cgatgtggga accgactatg cctcgctgat
      901 gggtgaactg atggcatact ctgacggaga gtacggcgga cgcttcatcg ctgctttgta
      961 tagcttggcc tttatcgaag atgacattcc aaccatgatt aataaagctc tggagttcat
     1021 tcctaaagag agccgctact acgaggtaat tcaagctgcc atcaaagctt gggagactta
     1081 cccggacagc tacagacgcg caagaagcga agtctaccgc aactactaca aagccgggca
     1141 gaccccttca agcggctggg tcattgctga tgtcaatggg gctatggtag ttctcgctct
     1201 cctctacggc cagggcgatt tcgaccagac gttggcactt gcggtgcagt tcggtttgga
     1261 caacgactgc aacgctgcca cagcaggtgg aatcctcggt gcgttcatcg gggcagaaaa
     1321 catcccagat cgctggaaga atccgcttaa caacctctac tacaacaacg tcctaagaca
     1381 ggctccatct tacctcctca tctctgacat tgccgacagg accgctaaaa tcggattggc
     1441 aaacgtcgaa aaacgcggag gtactgttga tctcgagaac aacaccatta ctctgccggc
     1501 ctacaaggtc gtatctcctg agtacaatga gccttggtcg gacgacaaag tagccggaaa
     1561 tatgctgcga ggctgggaag atggctggga aatggctaac ctcggatggg atatgagtcc
     1621 cggcatcaga ccggtttacg aaggcagaac caatgtcttt tccagccatc cgctggatca
     1681 aaaatcgcct ttctacttct acagaaccga aactctccca gcagataaaa tcactgtact
     1741 caaaacaggc gttaccagct ttaacggcga tgcccatccc actcacttcg aagctgattg
     1801 ggagcttaga gtgtacgtga acgatgaact gatcttctcc aagattatcg aaagaatcga
     1861 caaaaaaata gtttggtacg atttagaggt ttcgctcgcc gagtatgcag gacagaccgt
     1921 gacaatcagg gtagagaact atcctaacaa ctggtctttt gaagcaggat actgggacta
     1981 cttgaggctt gagcaaatag actagtaccg aaaaaaataa gggagtgctg ccagcttttc
     2041 caagctgtgc tgaagcactc ccttttttgc gtggaatttc gctaccagtc tggcttacga
     2101 caaccaggat tgggttagtc taggaatcca aaggtttcaa caaactcctt gatggccata
     2161 aagatcgctt cagcattctt agtagagaag tccccggact tctgccacac ctcaatgctg
     2221 tagttgctga caaagtctcc ttctgtaagg atggccggca ttgtccactg gcggattacc
     2281 gcaaaagacc tgtgtgcaaa gaagcggtat tccgatcctt ggtgttcgtt catgtacttg
     2341 atggacagcc tggctagctc gtggctgtag ggggtgtagt agtaggattc tgtgccgctt
     2401 gcagatctgt tggatgcagc gttgttgtga atgctgacaa acagatccgt ctggtatctc
     2461 tctgcaaaag ccaccctgtc ggataattcc ggtgcatagt tatagtcttt gtctctagtc
     2521 aggtagactc tgcaccctgc atcttctaga agttccttca gcttcagggc aatctccagg
     2581 ttgaggtctc tttccaaaag cccggtaggt cccacagcgc cggaatcggg accgtgtccc
     2641 gggtcaatag tgatcctctt gccttcgaga aatggcgtga acttgctttt gatccgcaga
     2701 ttcaggttgt ttcctgcaac gctcacatca taaccgtaga tagtgttccc cttaaggtcg
     2761 attctgtact ggagaatgtc atctgtaatc tgcatcgggg tgatcagctt cacaaaatct
     2821 gcgtcttttc tccatgccat gtggtaaagg ccgcttgtgg tattgtagaa ggttacaagc
     2881 aaagccggct cgtgaaccaa aggtgtaatg gtgtagaatg caggctccga catagtgaag
     2941 gtgacgttgg tccagtcgcc ttcttttgaa gccgtaatgt tgctgatcac aggttttgaa
     3001 ggtgctgtgc cttcctcaag gaagcggagg ttgctctggt ggatccacgc tgcataggtc
     3061 tttgctggag aaactctgta gtaatctcca actctggccg taacctcaac cttggcaatg
     3121 gtcttgatca tccccactct gtcgtactcc gttgacgggc cgctgtaaag gctcacagga
     3181 ttgttaggcg ctaagatctg agctaccact ggttttgcag gatcgtatac tgtaacacgg
     3241 gtgagccggc gtgtcgttgt gtttttcgct ttgtcggtaa gcgttacttc gatatacata
     3301 tcctcgccga tatcatcgct ctggaagcgg tagactccgc tgtagttccc agaggtaacc
     3361 tctgtcatgg gaaacggacc cttgtcccct atagagaagg tcacctcgca tcttgcttca
     3421 cttgcagtta gagtaaggtt caggatgtct ccaggtgaat acgccaagat ccccgtaggt
     3481 ttatactccc tgacttgggg tttggttgtg tctttctcga tgcgaacacg ctcggagatc
     3541 tcgttccctg ctaagtcctg gacatcgaag tagaagtaat ttctcccttc atcaaggggc
     3601 agccttagag agaatttccc gtccttatct gcttgaactt tgcgttcgtg ttgggtaacc
     3661 gctactgtgg caaaaggttc tgtactaccg cggacggtga tgtaatcttc agtggcaata
     3721 tagtcaatat ctgggaactc aatgtccaag aaaggcgcca ctgtatccag atttacctga
     3781 attacttgca tctccatatt gggcagcttt tcatcaaaga cagcaacata gatgaagttc
     3841 tcgccttctg tcaggctgac ttcggtgcga aacgagccat cttcaagaag cggatgcaca
     3901 gctccgttta taaacagacg acttttcggt tcggttttcc ccgaaaccgt tactctaccc
     3961 gtgttcacaa acgctccgtc tttaggttcg aagatttcca gtaacggtgg cactgtgtct
     4021 ctaataatgc gccgttgcag aatcaccgct tcgcctgttt tgctgctttg agctttgatg
     4081 cagatgatgt tctccccttc ctccaagctt acgacagctc gaaaggttcc atctgggaag
     4141 acgtctgcag gtttttctcc aacccaaacc aagcagtccg aatcggtttg cccttcaacc
     4201 gtgattgtgt ctttgtctgt tcgaggaatg tagtcaggtt tgaactccag tgccggatag
     4261 ggcgggtagt agacaacgag ttttggcact gcggctgcag tcaaaaccga tagtgacaaa
     4321 aagagcgtta agcacaacca aaatctcaat ctgacccgca tattcccaac tccttccatc
     4381 gttgtagttc aagaaacagc gatttttacc ttttcttcac taaaaaaacg acggcacgaa
     4441 aatcttagga tttcctgccg ccgtgctttg gctgtgatat tcaacgtata tgaacttaac
     4501 ataactaact aaagaccgaa gaaggccttc acttcatcta tcgtaggtag agatggctga
     4561 gctccttctc ttgtcaccga aacggccgaa gctctgctgg caagatcaat agcctcgtca
     4621 agggagagtc cccgtgccag tgccgcagcc agactgccgt tgaaacagtc tccggcagca
     4681 gtagtgtcta cagcatccac cttgtaggca ggaaagaatc tctcgccctg accgtcgatg
     4741 acaagcccgc cattgcttcc tcttgtcatg atcacggtag cacttgtagc ttccttcagc
     4801 tttctacacg caacaatagc ttcttcatcg gtgtccacag gaagaccggt caaagtctgg
     4861 agctcggtac tgttaggcgt gatgatatcc accatctgaa gaacctcatt gctgagtttt
     4921 gcagcaggcg ctgggtccaa aatcaccttg caaccttttt cctta
//
LOCUS       DAOSDA010000068         4960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_10696_length_4960_cov_3.428746, whole genome shotgun sequence.
ACCESSION   DAOSDA010000068 DAOSDA010000000
VERSION     DAOSDA010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10696_length_4960_cov_3.428746"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLW82_02460"
     CDS             complement(<1..377)
                     /locus_tag="PLW82_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46168.1"
                     /translation="MSAPTKEQKVKSVLTVYRNRDPVYGYRYVADPYSGCSQGCTYCP
                     QQDQTVEISINAAEVLSRQLAKKPRETVGIGDKCDPYMTLEKHYLITHDLLQVLAVQG
                     FPVHIVTRSTLVLRDRKILQEIAT"
     regulatory      complement(669..770)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(779..1576)
                     /locus_tag="PLW82_02465"
     CDS             complement(779..1576)
                     /locus_tag="PLW82_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ46169.1"
                     /translation="MKLPKFYLRRIWRTIMEFEMLEKGDKVVVGLSGGKDSLFLLYAL
                     KVIQTNSKFPFELAAATIDLGFGEINEPYLREYCERLSVPFYLEKTDIAAIVEEKAEE
                     KSPCSTCSFYRRGALVKVMQREGKNVLALAHHQDDAVETFLMSLLYSGQIKTFLPTTY
                     LDKSGIRVIRPLVYLTEAEVKGGLKYAEAKPVKNPCPYSGNTQREYVKNLLKTLIRDN
                     PNVYTHLLSAMREGKHIELWPKVKSRQEMHELYLRYMGAVDRDDYNS"
     gene            complement(1714..1872)
                     /locus_tag="PLW82_02470"
     CDS             complement(1714..1872)
                     /locus_tag="PLW82_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOJ46170.1"
                     /translation="MEKWVCSLCGWVYDPAVGDPDGGIEPGTPFEDLPDDWVCPLCGA
                     PKEEFERE"
     gene            complement(2028..3311)
                     /locus_tag="PLW82_02475"
     CDS             complement(2028..3311)
                     /locus_tag="PLW82_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46171.1"
                     /translation="MRKTVLMLAIILIVSVVGAAVEITAWITYGGLMGQILQELIEED
                     FTPSTGIKVNYQPYMVDSAFFQKALLALATGDAPDVMTLGAAQVIDLAIRGAILDLSE
                     FPDFDEAIADIYPGVMTALTFKDHVFGVPFEMGWTQAFYRTDIFSDLGISVPNTWDEF
                     KAIIPKIQAQGKDPWLSTIGNTADGHVRAFVPFIYQRGADLFTEDGLKSNLDSPEALE
                     AFKLFTSFYTDYKLPMELPEVQAFMSGDSPYMVTLNWIYAQIIRTQPQLQGKWTIAQA
                     PGTVGKDGKLDRTVPISAFAFAIPKSNKDEKVQAAWEFLKWIASDRVQKEFQKRIYES
                     PEEWYLVFGTRGTANSEIFPELERAVIDAALSESRGFTPVVGGYQTYRYLSFAFSRVV
                     MQNADPEAALLEAAKESNLELSRKQKEFERFVSKL"
     gene            3457..3711
                     /locus_tag="PLW82_02480"
     CDS             3457..3711
                     /locus_tag="PLW82_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HOJ46172.1"
                     /translation="MLFIVLGFIFGRKLKIGANATSRISSGILLLMLGALGYKVGSNP
                     EVVKNLSALGLNALVICMFTVSGSLLFVWLYLLGRKSRDS"
     gene            3701..4309
                     /locus_tag="PLW82_02485"
     CDS             3701..4309
                     /locus_tag="PLW82_02485"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HOJ46173.1"
                     /translation="MTVKIIAATVFGGIIGFIYPKYSSLVDPVISVFLLITMFFVGIE
                     FGRDKEFFKHLRESASFLVYPILIALGSLFGAAAAGLILPTGVLETVTVGSAFGWYSL
                     SAPLIGNLVSEELGATAFLANLSRELFTFILAPLLASSKLKGDDIKALTFAPAGATSM
                     DVTLPVILKAAGPKTAIAAFISGAVLSMLAPVLLQFFISLLI"
     gene            complement(4306..4896)
                     /locus_tag="PLW82_02490"
     CDS             complement(4306..4896)
                     /locus_tag="PLW82_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOJ46174.1"
                     /translation="MDKRLFLVGLIEGDLQRKALLLQKLFAERYGLYKSSLPQLHITL
                     EIVEEQSNLEFIQTAVMEAAGELERFPVRATGFACFPPPYKSVGLLIEKSREIERAAE
                     TLREHLAERGIESVRPFGTQFIYHLTLANTFLADNPWNEKEFLEACSIVDRVKAPLSL
                     VGTVNEIAIWRPLKDAEEMVVSSFALSEAGAVSKLR"
BASE COUNT         1217 a         1236 c         1108 g         1399 t
ORIGIN      
        1 gtggctatct cctgtaggat cttccgatcc ctgagtacta atgtgctgcg agtaactatg
       61 tgcacaggaa acccctgaac agcaaggacc tgcaacagat catgagtgat caaatagtgc
      121 ttttccaatg tcatgtaggg atcgcatttg tcgccgatcc caactgtctc gcgaggtttt
      181 ttcgctaatt gcctgctcaa cacctctgct gcattgatgc tgatttcaac agtctgatcc
      241 tgctgaggac aataagtaca cccttgagag caacctgagt acggatctgc tacatatcga
      301 tagccgtaaa ccggatctct attgcggtag acagtaagga ctgatttgac tttttgttct
      361 tttgtcggtg cactcattta taagcctccc ctctaccgca caattgttcg ttagactaaa
      421 gtctctctgc atgtaatatc ttaacacaga ctcggggaac attaaaggaa tgacaccata
      481 caatccgatg aaaaaagctg caggtttgcc tgcagcttaa ctccgattcc acgtagttat
      541 gattagccgt tccacctcct gttactttcc tccctcgcga agatgaagga gaaacatcac
      601 agtctgaacc ttcatagctt caaaccgggt tggcagaccc gtacgctata aagttgggta
      661 ggtgtacaga ctaaatgctg ctccctcata gtcaagcggt ttgcggccac ttggtagaga
      721 ctcccaagcc atattcctgg gattatacga ggaataccat aactacatca actttatttt
      781 aactgttata gtcatccctg tcaactgcgc ccatataccg gaggtaaagc tcgtgcatct
      841 cctggcggct tttgacttta ggccagagtt cgatatgctt cccctcccgc atagctgaaa
      901 gtaggtgggt ataaacattg gggttgtccc ggatcagggt ctttaagagg ttcttcacat
      961 actccctctg ggtgttcccc gaataaggac aggggttttt cacaggcttg gcttcagcat
     1021 acttcaggcc gcctttaact tccgcctctg taaggtagac taaaggccga atgactctga
     1081 tgccgctttt gtccagatat gtggtaggaa gaaaggtttt gatctgtccc gagtacaaca
     1141 ggctcataag aaacgtttcc acagcatcgt cttggtggtg tgcaagggct agcacatttt
     1201 taccctcacg ctgcatcact ttgaccaaag caccgcgcct gtaaaagctg caggtgctgc
     1261 agggactttt ttcttcggcc ttttcttcta cgatagctgc aatatctgtc ttttccaagt
     1321 agaagggcac agaaagtctt tcgcagtact ccctgagata tggttcattg atctcgccaa
     1381 aacccaaatc gattgtggct gcagccagtt caaaagggaa cttcgagttg gtttggatga
     1441 ccttcagagc atacaggaga aacagactgt ctttcccacc ggaaagacca acaacgactt
     1501 tatcgccttt ctccaacatc tcaaattcca taatcgtccg ccagattctg cgaagataga
     1561 acttaggaag cttcatagtt acacctctgt acagttagca taggaatatt ttagcggaac
     1621 tgggtgtaaa agcaacacaa aaatgggtgc ctgctgctgc agtcacccat ttttgtgtac
     1681 cacgttttaa gtacgatgtt ggagtctatc ctcttactct ctttcgaact cctctttcgg
     1741 tgcgccgcag agcggacata cccaatcgtc tggaagatcc tcaaaaggtg tgcctggttc
     1801 aataccaccg tctggatcgc ctactgctgg atcatatacc cacccgcata atgagcaaac
     1861 ccatttttcc atgtgacatc ctccttaact taaaatatct gttacctttt gattataccc
     1921 gaaaaatcaa aacataaacc ttattaaggt gaaaacccta tatttccctt tctacagcct
     1981 aatgttaaat tctgaaaact cccccggggt cgcgggggag tttattttta cagtttagag
     2041 acaaacctct cgaattcctt ctgctttctg gaaagttcca agttggattc tttagctgct
     2101 tccagaagag ctgcttctgg atctgcgttc tgcattacca ctctcgaaaa ggcaaagctg
     2161 aggtaacggt aggtttgata tccgcccacg actggtgtga atccgcggga ctcactgagg
     2221 gcagcgtcga tgactgctct ctcgagttct gggaagatct cggagttggc agtacctctg
     2281 gtaccgaaca ctagatacca ctcttctggc gactcataga tccgtttctg gaactctttt
     2341 tgtactctat cggaagcaat ccatttaagg aactcccaag cagcttgaac tttttcgtct
     2401 ttgttgctct tgggaatggc aaaggcaaat gctgagatag gaacagtacg atccaacttg
     2461 ccgtccttac ccacagtacc tggggcctga gcaatagtcc acttgccctg cagctgaggc
     2521 tgagtcctga taatctgagc gtaaatccag ttcagagtca ccatgtaggg cgagtcaccg
     2581 ctcatgaaag cctgaacctc tggcagctcc atgggcagtt tgtaatcggt ataaaacgaa
     2641 gtgaagagtt tgaatgcttc caaggcttca ggcgaatcca gattcgactt cagaccatct
     2701 tccgtgaaga gatccgctcc tctttgatag atgaacggaa cgaaagcacg aacatgaccg
     2761 tcagcggtgt tgccgatagt ggacagccat ggatccttgc cctgagcttg aatcttaggg
     2821 attatcgcct taaactcatc ccatgtattt ggcacgctga tgcctaaatc actgaaaata
     2881 tcagtgcggt agaaagcttg agtccaaccc atctcaaaag gaacaccgaa aacgtggtcc
     2941 ttaaaggtta gagcggtcat gactcctggg tagatatctg caatagcttc atcaaaatcc
     3001 gggaattcgg acaggtccaa aatagctccg cgaatagcca ggtcgataac ctgagctgct
     3061 cctagagtca taacatccgg cgcatcaccg gtggcaagtg ccaaaagcgc tttttggaaa
     3121 aacgctgagt ctaccatata aggttggtag tttaccttga tccctgttga cggagtgaag
     3181 tcttcttcaa tgagttcttg aaggatttgc cccataaggc ccccgtaggt aatccatgct
     3241 gtgatctcaa cagcagctcc tactacgctt acgataagta tgattgctag cattagaacc
     3301 gttttgcgca aaattaaccc cccctagcaa atttcgttta taatagaagt attatctatc
     3361 gttaggagtt ttcctgcaaa cgctgctctg ctcgtactca ttccgtgaaa aaaatctgct
     3421 gatcggggtg gtttcgtgaa ctttctgctc ctttgtatgc tttttattgt cttgggattc
     3481 attttcggga gaaagctaaa gatcggtgcc aatgccacca gccgcatctc cagtggcatt
     3541 ctcctgttaa tgcttggagc cttaggttat aaggtaggtt caaaccctga ggttgtaaaa
     3601 aacttgagtg cacttggctt gaacgctcta gtgatctgta tgtttacagt ttcaggaagt
     3661 cttctgttcg tttggttgta tctattgggg aggaagagtc gtgacagtta aaatcatcgc
     3721 agcaaccgtt tttggtggta tcattggatt catttatccc aaatactcgt cattagtgga
     3781 ccctgtgatc tccgtttttc tgcttataac gatgtttttt gttggtatcg aattcggccg
     3841 ggacaaggaa tttttcaagc atcttagaga gagtgcttcc ttcctcgtgt accccatact
     3901 tatcgccttg ggcagtctct ttggtgcggc ggctgccgga ttgattctgc caacgggagt
     3961 cctggaaacg gtgacagttg gctctgcatt tggctggtac agcctatctg cgcctttaat
     4021 cggcaatctg gtgtctgaag agctcggcgc tacagccttt ctagcgaatc tctccaggga
     4081 gcttttcacc ttcattctcg cgcctctctt ggcaagttca aaacttaaag gtgatgacat
     4141 aaaagcgctg acctttgcgc ctgctggtgc aacaagcatg gatgtcaccc ttcctgtgat
     4201 tttgaaagct gcaggaccga aaaccgccat tgcagctttc atttctggag cggtcctgtc
     4261 catgttggct cccgtgctcc tacagttttt catctccttg ctaatctagc gcagcttcga
     4321 caccgcacct gcttctgaca gggcaaatga acttaccacc atttcttcag cgtctttcaa
     4381 aggcctccag atagcgatct cgttaacagt tccgacaaga ctgagaggtg ctttgactct
     4441 gtcaacgata ctgcacgctt ccagaaactc tttttcattc catgggttat ccgccaagaa
     4501 agtgttcgcc aaagtcaggt ggtagatgaa ctgggttccg aaaggccgca ctgactctat
     4561 ccccctctct gccagatgtt ctctcagagt ctccgctgcc cgttcgattt ccctgctttt
     4621 ttcaatcaaa agccccactg acttgtacgg cggaggaaag caagcaaagc ccgttgcacg
     4681 gaccgggaat ctttcgagtt cacctgcagc ttccatcacc gcagtctgga tgaattcgag
     4741 gttgctttgc tcttcaacta tctctaaggt aatgtgcagc tgaggtaagc tacttttgta
     4801 aagcccgtat ctttctgcaa aaagtttttg cagaagaagg gcttttcttt gcagatcgcc
     4861 ctctatgagg cccaccaaaa aaagccgctt atccatgggc tcgcctccct tcttccaact
     4921 agtctgccct gaggagagtg ctttacccac atgtggagca
//
LOCUS       DAOSDA010000069         4934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_10787_length_4934_cov_3.621029, whole genome shotgun sequence.
ACCESSION   DAOSDA010000069 DAOSDA010000000
VERSION     DAOSDA010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10787_length_4934_cov_3.621029"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PLW82_02495"
     CDS             complement(<1..1015)
                     /locus_tag="PLW82_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ46175.1"
                     /translation="MLKWQKDLVIIGGGPAGLAAAIAARDEGIEDLIVLERHSELGGI
                     LNQCIHNGFGLHYFKEELTGPEYAERFIEELQKKNVPFLTDAMVISLSPDKKLLVASK
                     EHGLVEIEAKAVVLAMGCRERTRAAITIPGTRPSGVFTAGTVQALINIEGLMPGRRAV
                     IVGSGDIGLIMARRLTLEGAQVLAVVEIMDYPGGLNRNIAQCLEDFDIPLYLSHTIAD
                     IQGKDRVEKVLVAPVDENRQPILDKAFPLECDTLVLSVGLIPENELSLEAGVELDKTT
                     GGPIVSEYLETTTSGIFACGNVLQVHDLVDNVTAESIRAGKMAANYVKSGTLPACGTE
                     TKAG"
     gene            complement(1036..2505)
                     /locus_tag="PLW82_02500"
     CDS             complement(1036..2505)
                     /locus_tag="PLW82_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ46176.1"
                     /translation="MTEKTQRKKFDVLIIGGGVVGTAVARSLAKLKLKTALVEKEVEL
                     AFGTTKANSGIVHAGFHNEPGSLKAILCVKGCDMFPQVCEELDVPFKENGVLMVATSD
                     RELPLLHKFYEQGIQNGVRDLELIDRDRVFELEPNLSRDIVAGLFAPHGGIVSPFELA
                     IYQGVNAEKNGVEIFLDAAVENVVDRGNYIEVATKKGTFEAALVVNCAGLFADDIAQM
                     VGDTSFAIRPRKGEEYLLDKRVGNLVTSTIFPLPSENSKGILVIPTAHGNLMIGPTAL
                     EMQEREDLSTTQEGLEVIYQSASKLVKGISRRDIIASFAGVRAASDRGDFVIEFSKVA
                     PRLLNVAGIESPGLTAAPAIGEMVLEMIKDWCKDRGIPVEENHDFNPRLPKRVRLQDI
                     DDPRLEEMIAKDRRYAHAVCRCELVSEAEIVEAIQNGARTLDGIKLRTRAGMGRCQGG
                     FCTPKVLQIMARELNVPITSITKRGGDSVIAPYKTKELI"
     regulatory      2926..3025
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3109..3906
                     /locus_tag="PLW82_02505"
     CDS             3109..3906
                     /locus_tag="PLW82_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOJ46177.1"
                     /translation="MKKAKLYVVVFAVLILTATVFAKDVIKVGVNPGPHEELMEIVKG
                     ILAEKGIELEIISFADFITPNLALADGSIDVNSYQHEPFLLQFNKDRQTDLVKLAPTI
                     VFPISIYSQKVKSLSELKDKAIIAIPNDPTNAGRVLMLLEKAGLIKLKSGVGIQATVF
                     DIIDNPKNLQFRELEAAQIPRALPDVDAAAINTTYAVSAGLDPVNDPIYTEDKDSAYV
                     NILAVKRERLNDPLLQELVKAYHSKEVADYVEEKYKGSLVLGFTVPQ"
     gene            complement(4036..4725)
                     /locus_tag="PLW82_02510"
     CDS             complement(4036..4725)
                     /locus_tag="PLW82_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOJ46178.1"
                     /translation="MIGMGTLINSAAIVLGGLSGLVLGARLQEKTSETVMQALALVTI
                     FLGIETAFKGENMVAILLGLVVGGMVGEALQLETHLENLGKYVEGKLKAGKGNFAAGF
                     VTATILYVVGPMAILGSIEDGIGFGYKILLTKSALDGLSSIAFGASLGIGVPFAAVPV
                     FIYQGAITLIASFFGDVASASVIAAVSGCGGLLILAIGLNMLKLTKIRVTNLLPGIVF
                     APIFTWLLGLL"
     gene            complement(4805..>4934)
                     /locus_tag="PLW82_02515"
     CDS             complement(4805..>4934)
                     /locus_tag="PLW82_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOJ46179.1"
                     /translation="YKHTGQPLEEIQKNTERDNFMSAEEALKFGLVDGVMTERPKV"
BASE COUNT         1162 a         1293 c         1187 g         1292 t
ORIGIN      
        1 caccggcttt ggtttcggtg ccgcaggctg gaagagtgcc gcttttcacg tagtttgcgg
       61 ccattttgcc tgctctaatg ctctctgcag taacgttgtc cacaaggtcg tggacctgaa
      121 gcacgttgcc gcaggcgaag atgcccgaag tggtggtctc caagtactca gaaacgattg
      181 ggcctccggt ggttttgtcc agctcgacgc cggcctcaag cgacagctca ttttcaggga
      241 tgaggccgac agaaagcacc aaagtgtcgc actccagggg aaatgcctta tccaaaatcg
      301 gctgcctgtt ttcgtcgaca ggtgcaacca agaccttttc cacccgatcc ttgccctgga
      361 tatctgcaat ggtgtggctg aggtagaggg ggatgtcgaa atcctcgagg cactgggcga
      421 tgtttctgtt tagcccacct ggatagtcca tgatctccac tactgcaagc acttgtgctc
      481 cttcaagagt taggcgcctt gccatgatca gtccgatgtc tcctgaaccg acaataaccg
      541 ctcgcctgcc cggcatcagc ccttcgatgt taatgagagc ttggacggtg ccagcggtga
      601 agactccgct aggtcttgtt ccgggaatgg tgatcgctgc tcgagttctt tccctacagc
      661 ccatggcaag aacaactgct ttagcctcga tttctacaag gccgtgttcc ttagaggcca
      721 ccagcagttt tttgtcggga cttaaactga ttaccatagc gtctgtaagg aagggtacat
      781 tctttttctg tagttcctcg atgaagcgtt cggcatactc cggacctgtg agctcttctt
      841 tgaagtaatg caggccaaaa ccattgtgaa tacattggtt gagaataccg ccaagttccg
      901 agtgacgttc gagaacaatc aggtcttcga tcccttcgtc tcttgcggcg atggctgctg
      961 caagtcctgc tggtccgccg cctattatca ctaagtcttt ttgccattta agcattttct
     1021 tggggctcct tccaactaaa tcagttcttt cgttttgtag ggtgcaatca ccgagtcccc
     1081 gcctcgtttg gtaatgctgg tgatggggac gtttaactct cttgccataa tctgcagaac
     1141 tttgggagtg cagaacccgc cctggcagcg ccccatgccc gctctggttc tcagcttgat
     1201 gccatcgaga gtcctggctc cgttttggat ggcttcaacg atctccgctt cggaaaccag
     1261 ctcgcagcgg cagacagcat gggcatagcg tcggtctttg gcgatcatct cctcaagtct
     1321 gggatcatct atatcctgca agcgtactcg tttagggagg cgcggattga aatcgtgatt
     1381 ctcttctaca ggaatcccgc gatctttgca ccagtccttg atcatctcca gcaccatttc
     1441 gccaattgcc ggtgctgccg taaggcccgg agactctata cctgcaacgt taagcaacct
     1501 aggtgcgacc ttggagaact cgataacaaa gtcgcctctg tcgcttgcgg ctctgactcc
     1561 tgcaaaggaa gcgataatgt ctcttctgct gatccccttg accaacttag atgcagactg
     1621 gtagatgacc tccagaccct cttgggtagt ggagagatct tcgcgctcct gcatctccaa
     1681 agctgtaggt ccgatcatga ggtttccgtg ggcggtaggt atcaccagaa tgcccttgga
     1741 gttttctgaa ggtaggggga agatggtgct ggtgacaaga ttaccaaccc ttttatccaa
     1801 taggtactct tcaccttttc taggcctgat ggcaaagctg gtatctccca ccatttgcgc
     1861 aatgtcgtca gcaaacagac ctgcgcagtt aactaccagt gccgcttcga aggtgccctt
     1921 cttggtagcg acttcaatgt aatttccgcg atctacaaca ttttccactg ctgcgtcgag
     1981 aaagatttcc actccgtttt tctctgcatt tactccctgg tagatggcca gctcaaaggg
     2041 ggagacgatg ccgccgtgag gggcaaacaa gccggccaca atatctctcg aaagattagg
     2101 ttccagctca aaaacgcgat cgcgatctat taactcaagg tcgcgcacac cgttttggat
     2161 tccctgttcg tagaacttat ggagaagagg gagctcccga tcgcttgtgg cgaccatcag
     2221 cacaccgttt tctttaaagg gtacatccag ttcctcgcag acctggggga acatgtcgca
     2281 tccttttaca cagaggatgg ctttcaggct ccctggctcg ttgtgaaagc ctgcatgaac
     2341 aataccgctg ttggccttgg tcgtgccgaa ggccagttcc acttcctttt ccaccagtgc
     2401 tgttttcagt ttcaacttgg ccagacttct ggccacagca gtgccaacaa caccaccgcc
     2461 gatgatcagg acgtcgaact tcttacgctg agttttctcg gtcatctttt accacctcgt
     2521 ctctgttatt ttttctacaa cctccacctg tttacctttg gaaaaaaact ataacaacac
     2581 ccctgtgcag gggttctaga gatcgctctt ttcacctcaa gctacagaaa ccctgactgg
     2641 tgcatagctt agagtttccg tctgcaaggt tttttgtgat agggaaatag gtttacaaca
     2701 aaagcttacc acaggttttt tatctgggaa actcaatgca tttttacccg gttaggtgtc
     2761 gtcttagagt agccaagcaa agggttttgt tgaaaatgaa atcctttcct agcggcaatt
     2821 ccgaaggtcg tagagaaggc gggcgttttt tttcattttc gcaaaaacat cttgacaagt
     2881 ctccgcttat tgtgatagat ttacagttaa caaattagct gtaggctctt atccagagtg
     2941 gcggagggac tggcccgatg aagcccggca acctgcaatg caaggtgcca aatcctgcga
     3001 attctcggaa ttcggaagat aagagggagt agacatcgtt gcttcctctc ttgaggaagc
     3061 tttttttcta ccctggatag agtgtagagc accaatggag gtacaaacat gaaaaaagct
     3121 aagttgtatg ttgttgtttt cgctgttttg atccttacag ctaccgtttt tgccaaagac
     3181 gtgatcaagg tgggcgtcaa ccccggacct cacgaagagc tgatggagat tgtaaaagga
     3241 atactggcag aaaagggcat cgaacttgag atcatatcct tcgccgactt catcacgccc
     3301 aatctagctc ttgccgacgg ttccatcgat gttaacagct accagcacga accgttcctc
     3361 ctccagttca acaaagacag acaaaccgac ctggtgaagc ttgcaccgac cattgttttc
     3421 cccatcagca tctattcgca gaaggtaaaa tccttaagcg aactaaagga caaggccatc
     3481 attgccattc ccaacgatcc aaccaacgcg ggcagggtct tgatgcttct ggaaaaagcc
     3541 ggcttgatca agctgaagtc gggagttggt atccaagcta cggttttcga tattatcgat
     3601 aacccgaaaa accttcagtt cagagagttg gaagcagcac agatccccag agctctacct
     3661 gatgtggatg cagctgctat caacactact tacgcagtct ctgccggttt ggatcctgtt
     3721 aacgatccca tctacaccga agacaaagac tcggcgtatg taaacatatt ggccgtaaag
     3781 cgggagcgcc taaacgatcc tctccttcaa gagctggtga aggcttatca ttccaaagaa
     3841 gttgccgact acgtagagga gaagtacaaa ggctctctgg ttctaggttt cactgtccca
     3901 caataaaagc tgtgcgaagc tgatgccttg acacggctgt gacaaaaaag gagcagttca
     3961 aaagcgactg ctcctttttt atgagggaaa cggatccgtt acttttgacg cccaaatctt
     4021 ggaatccgtt gcctcttaca atagtcctaa aagccaggta aagatcggtg caaacacgat
     4081 gcccgggagg agattggtga cccggatttt tgtcagcttc agcatgttca gccctatcgc
     4141 caggatcagc agcccaccgc atcccgaaac tgcagcaatg acgcttgcag aagctacgtc
     4201 tccgaagaag ctagcgataa gggttattgc accctggtag atgaaaaccg gtactgcagc
     4261 aaagggcacg cctatcccca gggatgctcc gaaggctatg gaggacagac cgtcgagggc
     4321 agatttggtt aggaggattt tgtagccaaa gccgatgccg tcttctatgg agccgagaat
     4381 ggccataggc cctactacat agagaatggt cgctgtaaca aaacctgcgg caaagttgcc
     4441 ttttccggct ttaagcttac cttctacgta cttgcctaag ttttccagat gggtttccag
     4501 ctgcaaggct tctcccacca ttcctcccac aaccagtccc aagagaatgg ctaccatgtt
     4561 ttcgccttta aatgccgttt ctataccgag aaaaatcgtc accagagcca gtgcctgcat
     4621 gacggtttcc gaagtctttt cttgcagtct tgccccaagc acaaggccgg aaagtccgcc
     4681 aagcacaatg gctgccgagt tgatcagcgt tcccatacct atcattctag tcaccttcta
     4741 ttcccattct ggtgcggcgt acataaaaac agcgcctcgc acggaggcgc tgtagagccc
     4801 atctttatac tttcggcctt tcggtcatga ctccatctac aagaccgaat tttagtgctt
     4861 cctctgcgct catgaagttg tctctttctg tgtttttctg gatctcttcg agaggctgac
     4921 cggtatgctt gtag
//
LOCUS       DAOSDA010000070         4891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_10885_length_4891_cov_2.925352, whole genome shotgun sequence.
ACCESSION   DAOSDA010000070 DAOSDA010000000
VERSION     DAOSDA010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10885_length_4891_cov_2.925352"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2099)
                     /locus_tag="PLW82_02520"
     CDS             complement(<1..2099)
                     /locus_tag="PLW82_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOJ46180.1"
                     /translation="MFLLIFILAFSSAATELYRLGPGDQLIITVWERPDLRTDVVVGP
                     DGRIAVPLAGQLHVDGYTLEEVEKALVERLQVYLLDPLVTVQVAKYRTFRVQVLGNVA
                     RPGFYNLEYGSTLLDALAAAGGPLQTADLEHIKVGDLEINAANILQNPSLDMELTAGT
                     TISVPAAKPVLVLGAVKNPGTYSLPLSTADATVFDVIALAGGLTLNADENQVRVERYG
                     KETVYEGALKEIGDKMVQPGDTLVVAEKRQVVISGKVLRPGSYQIASGMGVADAIALA
                     GGLLQDASQEVTIYKDSETVVTSVYDRTPLTGGESLVVGTSTNMVLVLGEVVKPGNYA
                     VAQANTLLEAISLAGVPTNFADLRQVTVGERTVDLAKVLRDPSLDVTIGSGTIIFVPK
                     TSPVLVLGAVKNPGTYLPTGEQTLLEIIAQAGGLLDTADDSAISLQRGGEETVGSLTR
                     LGSMVLQNGDVITIPERQQIIVAGQVVRPGAYEVRKGLTVSGALALAGGLQQDAAKTL
                     TVVKDGVSREVPVDSMEVLTGGESLFVGISYQRVLVLGEVARPGSFVWHEGLTVWDVL
                     ALAGGQTESSDLQSAFITRNGEILPVDWSKNEPLRGGDILEIPKWNRQVLVMGEVARP
                     GTYTVERNQKLLDVIGLAGGLTQKADYELVVRKDDQFKTINIASALANPADPANVVIS
                     GGEVIFVGEARSTVLVFG"
     gene            complement(2270..3187)
                     /locus_tag="PLW82_02525"
     CDS             complement(2270..3187)
                     /locus_tag="PLW82_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46181.1"
                     /translation="MKRRLTILLVSLILVLPVLTAAAELPPTHQALAEIEVMLYGGAS
                     DGPLVNRLNAIEQDLFGVTKSGAFVQRLASAYNFITGESLTEGSIVLKLNVIEWALFQ
                     KLSTGSIVSRIGEIESQFYGQLSAGSVFERINRISNDIFPTNSFNVETVKVSSGTVVR
                     VRINIALSSEDTKPGTVIPYTVAEDVVINERLVIPAGAQGRGTVINVTPLGSMGKHGT
                     VEISWGSLTAIDGTRIDVGMTEKSIAAFNTSYSDLKASASVAGGVVITVEGTAPGFMS
                     PGKALILPEGTRFPIEVKYEARVGGVMFN"
     gene            complement(3203..4453)
                     /locus_tag="PLW82_02530"
     CDS             complement(3203..4453)
                     /locus_tag="PLW82_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46182.1"
                     /translation="MTRKLNTALIIILFVALTCVGLASGQDQEKDHEFKVLIGSGLLN
                     ADEAFGVLRAENSSPYELASLVGRAWTRVSGTKTDSLSARIVIDVPSIVKKLPELKNQ
                     YSNFTLETLESLMGLTYKYRSELSDLGYGFYFGRFAVYQGQDNLTLYGLWDEDREVDL
                     ILTEMVKPFVASYTNEIADWARFRLEYVSLSEVNKRLAGEIQLGNLALGGALLANNDE
                     SYRIEAALRFGSVQLGAGVTAGADIQGNRVFTLPDLGFSFKLGEFHLATYNAPLYGYV
                     QPNRSQNREDSLFSPPNLREQIEVPQVTSLVVGIPINEKAVLRAGLLYGGVNDQEKGI
                     AITKGASAGITFDILQANVQADYQYYSRDNEEESNTARLKIGYGITDSTYINLAYRLV
                     TSSEKLTGKTADENLATAEISIQF"
     gene            complement(4548..>4891)
                     /locus_tag="PLW82_02535"
     CDS             complement(4548..>4891)
                     /locus_tag="PLW82_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46183.1"
                     /translation="VDSVGRVVLQKIVPSNELIAEIEGISKGKRITVVMGNGTTARRY
                     LPLVEELDLPVELVEEGYSTLEAKELYFRENGYGWQVILPKGLRSVRRPLDDYAARIL
                     VDRYLNSRKNM"
BASE COUNT         1198 a         1318 c         1084 g         1291 t
ORIGIN      
        1 ccgaagacca gcaccgttga tcttgcctcc ccaacaaaga ttacctctcc gccgcttata
       61 acaacgtttg caggatccgc tgggttagcc agagcactag cgatgtttat ggttttgaac
      121 tgatcatctt ttctgaccac tagttcgtaa tcggcttttt gtgtgaggcc tccggcaagg
      181 ccgatgacat caagcagttt ctggtttctc tccaccgtat aggtgcccgg gcgagctacc
      241 tcacccatca ccagaacctg acggttccac ttgggtatct caaggatgtc tccgccacga
      301 agcggttcgt tcttcgacca atcgaccggc aaaatttcac catttctcgt gatgaaggcg
      361 ctctgcagat cgctggactc ggtctgtccg cctgcaaggg caagaacgtc ccaaacagtg
      421 agtccctcgt gccagacgaa actgcccggc ctggcaactt caccgaggac tagaactctt
      481 tgataggaga ttcccacaaa gagtgactct ccgccggtga ggacttccat ggaatccaca
      541 ggaacttctc tgctgacgcc gtctttgaca acagttagag tctttgcagc atcctgctgc
      601 aaacctcctg caagggctag agcacctgag acggtcagcc cttttctaac ctcgtaggca
      661 cctgggcgca caacctgtcc ggcaacgatg atctgctgcc tttcaggaat agtaatcaca
      721 tctccatttt gcagtaccat ggaaccgagt cttgtgaggc tgcctactgt ctcctcaccg
      781 ccgcgctgca gggagattgc actgtcgtcg gcagtgtcaa ggagtccacc agcttgagct
      841 attatttcaa gtagggtttg ttctccagtg ggcaggtagg ttcccggatt cttgaccgct
      901 cctaaaacca gaacgggact ggttttcggc acgaagataa tcgtcccgga accgatcgta
      961 acatccaggc tggggtctct taaaaccttc gccaaatcca cagtcctttc cccaactgta
     1021 acctgtctca ggtcggcgaa gttggttggt acaccagcta gggatatggc ttcgagtagg
     1081 gtgttcgcct gtgctacagc gtagtttcct ggctttacaa cctctcccaa aaccagcacc
     1141 atgttggtgg atgtccccac aaccagcgac tctccgccgg taagaggggt tctatcgtaa
     1201 accgaagtaa cgacggtttc actgtctttg taaatggtta cctcttgaga tgcatcctgc
     1261 agcaaacctc ctgcaagtgc tatggcatct gctactccca ttcccgaagc tatctggtag
     1321 gaacctggtc tgagcacttt cccggaaatg actacctgtc tcttttcagc cactaccagc
     1381 gtatcgccgg gttgaaccat cttgtcgccg atctctttga gggcaccttc ataaacggtt
     1441 tccttcccgt atctttcaac cctgacttgg ttttcatcgg cgttgagagt aagtccgcct
     1501 gcaagggcaa taacatcaaa tactgtggca tcagcggtcg aaagaggaag actataagta
     1561 ccaggattct tcaccgcacc taacaccaga actggctttg cagctggtac gctgatggta
     1621 gttccggccg taagttccat gtctaggctg ggattctgca ggatatttgc agcgttaatt
     1681 tctagatctc caaccttaat atgttctaag tcagcggtct gcagcggtcc tccagcagct
     1741 gctaatgcat ccaaaagggt gctgccatac tccaagttgt aaaagccggg tcttgcaaca
     1801 ttgcccaata cctgtactct gaatgtacgg tacttggcaa cctgcacagt caccaaagga
     1861 tccaaaaggt agacttggag cctctctaca agagctttct caacttcttc tagcgtatat
     1921 ccatcaacgt gtagctgtcc tgcaagagga acggcgatac gcccatcagg accaacaacc
     1981 acatctgtcc gcaggtcggg tctttcccaa acggtaatga tcagctgatc acctggtccc
     2041 aatctgtaca gttcggttgc tgctgagcta aacgctaaaa tgaatattaa taagaacaat
     2101 aatccatgtc gaatcgatct cttgtgcatc gtcctacctc caaaagcgtc ctcaagagtt
     2161 gtccaaaagc ttacttctta ttgtttccac atttccctgc caatccctct gcacaaacca
     2221 aataaaagca aaaatagagc cgttttgaac ggctctatgc ttgtttcttc tagttaaaca
     2281 tgacgcctcc gactctcgct tcgtacttga cctcaattgg gaagcgcgtt ccctcaggta
     2341 agatcaacgc tttgcctgga gacataaaac caggagctgt tccttctact gtgatcacaa
     2401 ctccacctgc aactgacgcg gatgccttta ggtcggagta gcttgtgtta aatgcagcta
     2461 tggacttttc agtcatgcca acgtcgatgc gagttccgtc gattgccgta aggctacccc
     2521 aagatatctc cacggtaccg tgcttaccca tgctgcctaa cggtgtcaca ttgattaccg
     2581 ttcctctacc ttgagcacct gcaggaatca ccagccgctc attaatgacc acatcttcag
     2641 ctactgtgta cggaatcacc gttccgggtt ttgtatcctc agagctcagt gcgatgttta
     2701 ttctaaccct aacgactgta ccgctggaaa ccttaacggt ctctacattg aaactgttgg
     2761 tcggaaagat atcgttactg atcctgttga ttctttcaaa tacgctgcct gcagacagct
     2821 ggccgtagaa ctggctctca atctcaccta tacggctgac gatggatccg gtggaaagct
     2881 tttgaaagag agcccactca attacgttga gttttagaac tatggaacct tcagtaaggc
     2941 tttctccggt gatgaagttg tacgctgagg ctaagcgctg gacgaacgca ccagacttgg
     3001 tgacgccgaa cagatcctgt tcaatggcgt tcagtctatt taccagaggt ccgtcgctag
     3061 ctccgccgta aagcattact tctatttcag caagagcctg gtgagtaggt ggaagttcag
     3121 cggcagctgt caagaccggt aaaaccaaaa tcaaagatac caaaagaata gtcagccttc
     3181 ttttcattga agttcctcct ctttaaaact gaatactgat ctctgctgtg gctaaatttt
     3241 catcagctgt ctttcctgtc aacttctccg aagaagtaac taaacgataa gcaagattga
     3301 tataagtgct gtcggtgatg ccgtagccaa tcttcagacg tgccgtgtta ctttcctctt
     3361 cgttgtctct gctgtagtac tggtagtctg cctggacgtt ggcttgcagg atatcgaaag
     3421 tgattcctgc ggaggcacct tttgtaatag ctataccttt ctcctggtcg ttaactcctc
     3481 cgtagagcaa tcctgctctt aaaacagctt tctcatttat tggaatgcct acgactaaag
     3541 aagttacctg aggaacttca atttgttcgc gtaagtttgg aggggagaac aaactgtctt
     3601 ctcgattctg actcctattg ggctgtacgt acccgtagag gggggcgtta taagtagcca
     3661 aatgaaactc tccaagtttg aaggagaaac caaggtcagg cagagtgaac accctgtttc
     3721 cctgaatgtc tgctccggca gtcaccccag cccctaattg aacagaacca aatctaaggg
     3781 ccgcttcaat acggtaggac tcatcattgt tagcgagcaa agctcctccc agagctaagt
     3841 ttcccaactg aatctcgcct gcaagcctct tgtttacttc agaaagagac acgtactcca
     3901 atctaaaacg agcccaatcg gcaatctcat ttgtgtagct tgcaacaaat ggcttcacca
     3961 tctcggtgag aatgagatcg acctctctat cttcatccca caatccatac agcgtcaggt
     4021 tgtcctgacc ctgatagaca gcaaacctcc cgaagtaaaa gccgtaaccc aaatcgctga
     4081 gttcgctgcg atacttgtaa gtcagaccca tcagcgactc taaagtctca agggtgaagt
     4141 ttgagtattg gttcttcaac tcagggagct tcttgacaat ggaagggaca tcaataacaa
     4201 ttctagcgct gagactgtcg gttttagtac cgctgaccct ggtccaagct ctccccacga
     4261 gacttgcaag ttcatagggg ctgctgtttt ccgctcgcaa tacaccaaaa gcttcatcgg
     4321 cgttgagcaa tcctgagccg atcagcacct taaactcgtg atctttctct tggtcctggc
     4381 cagaagctag accgacgcaa gtcagtgcaa caaaaagaat gattataagg gcggtattta
     4441 gttttctggt catttcgcgc caccctcttt gttatcaaga atacaaatgg taatgttata
     4501 tcttctcgta ggaagaagat tattccttcc tgacgaaacg gtgcgactca catgtttttg
     4561 cggctgttta gatacctatc taccaggatt ctggcagcat agtcgtctag aggacgcctt
     4621 acgctccgca atcctttagg caggatcacc tgccaaccgt agccgttttc tcggaagtag
     4681 agttcctttg cctcgagcgt cgaataaccc tcctcaacga gttcaaccgg gagatccaac
     4741 tcttcgacaa gaggcagata ccgccttgct gtagtgccgt ttcccatcac aactgttata
     4801 cgcttcccct tacttattcc ctcgatctca gcgatgagct cattagaagg cacgattttc
     4861 tgaagcacta cacggcctac actgtccacc a
//
LOCUS       DAOSDA010000071         4880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_10906_length_4880_cov_3.414301, whole genome shotgun sequence.
ACCESSION   DAOSDA010000071 DAOSDA010000000
VERSION     DAOSDA010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10906_length_4880_cov_3.414301"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1134)
                     /locus_tag="PLW82_02540"
     CDS             complement(118..1134)
                     /locus_tag="PLW82_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ46184.1"
                     /translation="MKILQTQNLKKHFPIRSGVFLQITGYVKALEKVDFEISEGETIG
                     IVGESGCGKSTLGKTLVKIYEPTAGSITYFDPTGKVHDITKGLRKDVRSIFRKDVQMI
                     FQNPYDSLDPRMTIRDIIREPIETHRLFNTSQAMDDYVRELLTKVGLDPGYGKRYPHE
                     FSGGQRQRIAIARSISVKPRLLICDEPTSALDVSVQSQIINLLKQIQRENQMAFIFIS
                     HNLDLVYHMSDRILVMYLGNIVESAPSTAIFDSPAHPYTQALMSAIPSWNPTERKLKD
                     VKLEGEPPSPINPPHGCPFHPRCQYRFDACSTVKPDPVMVSPGHICACHLHAEKQKDE
                     KIVS"
     gene            complement(1106..2122)
                     /locus_tag="PLW82_02545"
     CDS             complement(1106..2122)
                     /locus_tag="PLW82_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46185.1"
                     /translation="MTDAKRVLTIQNLRTYFKTPNGLVKAVDDVSFHLDKGEILAIVG
                     ESGCGKSVTAQSILGLIKVPPAQMSGSIVFKGKELIGSSADEYRKIRGMHISMIFQEP
                     MSAFDPLYTIGQQLMEVAKAHMPWSESEAKERIIDSLKKVHIPEPEKRYNEYPHEMSG
                     GMLQRIMIAMALLTSPEIIIADEPTTALDVTIQAQVLNLMQELQESYNGSIIFITHDL
                     GVVAEVADRVLVMYAGKIVEKASVYDLFANPLHPYTSGLLTSRVKREYKKEGKDLPYI
                     KGYVPKADEFSQGCRFNTRCPYKTEKCEKETPPEIEATSGHTVACFLYEGSDSNENLA
                     NTKP"
     gene            complement(2119..2988)
                     /locus_tag="PLW82_02550"
     CDS             complement(2119..2988)
                     /locus_tag="PLW82_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ46186.1"
                     /translation="MKRSRDPVTFQVVFTEDTTKSVPIKFFIRGEEYRFLGLFKTNLH
                     LFGVEATPDKTMLLLFGADRFGRDVFSRVLVGGRVSMTVGLLGTFLSVCIGAIVGALS
                     GFYGGKVDVLIQRFIELLRSFPRIPLWLALAVIVPPSWPSTWVYFGIVTVLSLIGWMG
                     VARVMRGLVLSLKEKEFVLAAKVIGVSDMKIIIKHLIPNTLSYLIVVSTLSIPGMILG
                     ESSISFLGLGIKEPMTSWGLLLKQAQKFSELEAHPWLMIPGIFIMIAVLSFNFVGDAL
                     RDAVDPYRTVEKL"
     gene            complement(3058..3210)
                     /locus_tag="PLW82_02555"
                     /pseudo
     CDS             complement(3058..3210)
                     /locus_tag="PLW82_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(3226..4194)
                     /locus_tag="PLW82_02560"
     CDS             complement(3226..4194)
                     /locus_tag="PLW82_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ46187.1"
                     /translation="MWAFIVRRILIMIPMMLLASIICFIIIELQPGDFVSQYLDNPRI
                     SPEQIRLLRERLALDKPAYIRYFNWIKGIVTKWDFGYSFAYERPVGELIFERMGWTVT
                     IALLTILFQWILAVPMGIYSALKPYSPGDYIMTVTGFIGISIPDFFFALVLMFFMLKM
                     GSTSIGGLFSSRFIGAPWSFAKFVDLLKHIWMPLLVVGFSGLAGLMRVMRGNMLDVVG
                     SPFITSLRAYGLPEKKIRRHAIKNALNPMVSIAGSELPNVFSGTIITAIVLNLPTMGP
                     FFYDALLNHDQYLVMAFLMFIALITQIGNLLADVALALLDPRIRMS"
     gene            complement(4269..>4880)
                     /locus_tag="PLW82_02565"
     CDS             complement(4269..>4880)
                     /locus_tag="PLW82_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ46188.1"
                     /translation="PEKAKAILDQLGLKDTNKDGIREFADGKPVEFTLTVQSSPQDYQ
                     DIALIFKEDVEKAGIKVNLQILESSLTGNMFGAGSFEAGIRAFGNQPDLELRKAIWQP
                     GTQLYYWHYSVLDKESLVAITDKMFDWEVRLYELFDLGSITTDVEKRKLVYDEVQEIY
                     HDVLPVIFVSKEMNLWGASKSLGNVYQDAEGVVYFSTYTCYKK"
BASE COUNT         1332 a         1127 c         1112 g         1309 t
ORIGIN      
        1 gatttgaaac ctgaagttat tgctgcataa tgtccaaagt taggggtttg aaccggtaaa
       61 ttcatgtacc agattcagtt gaaaacggat tttgtccaga agcttttaac acagctttca
      121 gctcactatc ttctcgtctt tttgcttctc tgcgtgtagg tgacaagcac agatgtgccc
      181 aggtgaaacc ataactgggt ccggcttaac cgtactgcaa gcgtcaaagc gatactggca
      241 ccttggatgg aaaggacaac cgtggggcgg attgatcgga ctaggcggct ccccttcaag
      301 cttgacgtcc ttaagtttgc gttctgtagg attccagctt gggattgcag acattaaagc
      361 ctgagtgtag ggatgagcgg gactatcaaa gatggcagtt gaaggtgccg actcaacaat
      421 gttacctaag tacattacca agatgcggtc gctcatgtgg tagacaagat cgaggttatg
      481 agagatgaag ataaaagcca tctggttttc tctctgaatc tgctttaaga gattaatgat
      541 ctgactttgc acggaaacat ccaacgctga ggttggttca tcgcagatta aaagccttgg
      601 ttttactgag attgaacggg caattgcaat acgctgcctc tgacctccag aaaactcgtg
      661 agggtaccgt ttaccgtagc ctggatcaag cccgacttta gtaagaagtt cacgaacata
      721 gtcgtccata gcctgacttg tgttaaacaa cctatgggtt tcaataggtt cccggatgat
      781 atcgcggata gtcatgcgcg gatcgagaga atcataaggg ttttgaaaga tcatctggac
      841 atcctttcgg aagatcgagc ggacgtcttt gcggagtccc ttagtaatat catggacttt
      901 tccggtagga tcaaaatagg taatgctgcc tgcagtgggc tcgtagattt tcactaaagt
      961 cttgccaaga gtgcttttgc cgcatcctga ctcgcctaca ataccgatgg tttcaccttc
     1021 acttatttca aaatcaacct tttccaacgc tttaacataa ccggtgattt gaaggaacac
     1081 tcctgatctg atagggaagt gcttcttaag gttttgtgtt tgcaagattt tcatttgaat
     1141 cactcccttc atataaaaag caggcgacag tgtgtccgga agttgcttca atctccggag
     1201 gagtttcctt ctcacatttt tcagttttgt aaggacatct ggtgttaaaa cggcagccct
     1261 gagagaactc atctgcttta ggtacatagc ctttgatgta gggaagatcc tttccctctt
     1321 ttttatactc acgtttgacc cgtgaagtta aaagaccgga ggtataaggg tgcaggggat
     1381 ttgcaaagag gtcgtagacc gaagcttttt ccacaatctt tcctgcatac attacgagga
     1441 cgcggtcagc aacttccgcc acaacaccaa gatcatgagt gataaagatg atcgagccgt
     1501 tgtagctttc ctgaagctcc tgcattaagt tcagaacttg cgcctggata gttacatcaa
     1561 gtgctgtggt aggctcatca gcaatgatga tctccggact tgtgagaagg gccatggcga
     1621 tcatgattcg ctggagcata ccgccgctca tctcatgtgg gtattcgtta tagcgcttct
     1681 ccggttcagg gatatgcact tttttcagtg agtcaatgat tctttctttc gcttcagact
     1741 cgctccaagg catgtgagcc ttcgctacct ccatcagctg ctgacctatg gtgtagaggg
     1801 gatcaaaagc tgacatcggt tcttggaaga tcatgctgat gtgcatgcct ctgattttgc
     1861 gatactcatc ggcactagac ccgatcaact cttttccttt aaaaacaata ctaccgctca
     1921 tttgcgcagg cgggaccttg atcagtccta agatagactg agctgtgacg cttttgccgc
     1981 agcccgattc gcctactatc gctaagatct cacctttatc aagatggaag ctcacatcat
     2041 cgacggcctt cacaaggccg tttggagttt taaaataagt cctcaggttc tgtatggtta
     2101 gaacacgctt tgcatctgtc atagtttttc aaccgtcctg tagggatcaa ctgcgtctct
     2161 tagagcatcg cctacaaagt taaacgagag cacagcgatc atgatgaaga taccgggtat
     2221 catcagccaa gggtgagctt ccagttcact gaacttctga gcctgcttga gaagcagtcc
     2281 ccaacttgtc ataggctcct tgatccctaa acccagaaac gatatcgagc tttcaccgag
     2341 tatcattcct ggtattgata aagtagagac tacaatcaga tagcttaagg tattgggtat
     2401 taagtgcttg attattatct tcatatcgga aacaccaatg acctttgcag ctagtacgaa
     2461 ctccttttcc ttcagactga gtaccaaacc tcgcataact ctggcaactc ccatccagcc
     2521 gatgagcgaa agaacagtga cgataccgaa atatacccag gtgctcggcc agcttggcgg
     2581 gacgataaca gctaaggcaa gccagagcgg tatcctcgga aacgatctta agagctcgat
     2641 gaacctctgg attagtacat cgactttacc gccgtaaaac ccggatagag ctccgacaat
     2701 tgccccgata caaacactga gaaatgtccc taggagacct acggtcattg agacccgacc
     2761 tccgaccaaa actcgagaga acacgtctct gccgaagcgg tctgctccga atagtagcag
     2821 catagtcttg tcgggagttg cctccacccc aaagaggtgg agattggttt taaacaagcc
     2881 taagaaacgg tactcttcac cacggatgaa aaacttaatc ggtaccgatt tggtagtgtc
     2941 ttcagtgaaa acgacctgga aggttacggg gtcacggctc cttttcattt catagatatg
     3001 tggtccgatc catttgcctt cctgatctct aaacctgatc tttgaaggtg gagcatatac
     3061 gaagttagta tgtgtttcag taaagttgta cggagctata aaatcagcga agagaaccaa
     3121 aagatacaaa atacagagta ctatcagacc gaaaacacct aaccggtgct taaaaaagct
     3181 ttgtaaaacc cgttttttag ttccttccat gattccacct ctttcctagc tcattctgat
     3241 tctgggatca agcagtgcaa gtgctacatc ggcaagcaga ttgccgattt gtgtaatcag
     3301 ggcaatgaac attagaaagg ccatgaccag atactgatca tgattgagca gagcatcgta
     3361 gaagaatggt cccatggtag gtaggtttag aacgatagca gtgatgatcg taccactgaa
     3421 aacatttgga agctcgcttc ctgcgatact gaccattgga ttgagagcgt ttttgatcgc
     3481 gtgacgcctg atctttttct caggcagccc gtaagctctt agagatgtga taaacggtga
     3541 acccacaaca tcaagcatgt tcccgcgcat gactctcata agacctgcaa gtccgctgaa
     3601 gcccactacc agaagaggca tccaaatatg cttcagtaaa tccacaaact tggcaaaact
     3661 ccaaggtgct ccaataaacc gggaagaaaa caacccgccg attgaggtgg agcccatctt
     3721 cagcatgaaa aacattaaaa ctaacgcgaa gaaaaagtcc ggaatggata tgccgataaa
     3781 cccggtgaca gtcatgatgt agtctccagg cgagtacggc tttaaggccg agtagattcc
     3841 cataggcact gcaagtatcc attggaagag gatagtaagc agtgctatag tcactgtcca
     3901 gcccattctt tcgaagatca gttcgccaac tggtctttca taggcaaagg agtaaccaaa
     3961 gtcccactta gtaacaattc ctttaatcca gttgaagtat ctgatgtaag ctggtttgtc
     4021 gagagcgagt ctctcacgga gcagtctgat ctgttcaggt gaaatcctcg ggttatctaa
     4081 atactggctg acaaaatctc caggttgcag ctcgataatg ataaaacaga ttattgatgc
     4141 taaaagcatc atggggatca tgatcaggat tcgtctgact ataaatgccc acatcaagtc
     4201 ctcacctcta aaaaccaaaa cttgcaaaga gaggggaaag ccggatgctt tcccccattt
     4261 aacccggctt atttcttgta gcaggtatag gttgagaagt aaactacacc ttctgcatcc
     4321 tggtagacgt tgccgaggct ctttgaagcg ccccagagat tcatctcttt ggatacaaag
     4381 atgaccggga gaacatcatg gtagatctcc tgaacttcat cgtagactag cttgcgtttt
     4441 tccacatcgg tggtgataga accaaggtcg aacagctcat agagtctgac ttcccaatca
     4501 aacatcttat ctgtaatagc tactagactc tctttgtcga gtacggagta atgccagtag
     4561 taaagctgag ttccgggctg ccagatagct tttctcagtt cgaggtccgg ctgattaccg
     4621 aaagctctga tacctgcttc gaagcttcct gcaccaaaca tgtttcctgt cagtgagctt
     4681 tccagaatct gcaagttgac tttaatacct gctttctcaa catcttcttt gaagatgaga
     4741 gcgatatcct ggtagtcctg aggtgaactc tgaactgtaa gagtaaactc cacaggctta
     4801 ccatcagcaa actctctgat accgtctttg ttggtgtcct taagcccgag ctgatctaag
     4861 atggctttgg ctttttcagg
//
LOCUS       DAOSDA010000072         4862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_10967_length_4862_cov_3.293530, whole genome shotgun sequence.
ACCESSION   DAOSDA010000072 DAOSDA010000000
VERSION     DAOSDA010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10967_length_4862_cov_3.293530"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLW82_02570"
     CDS             complement(<1..241)
                     /locus_tag="PLW82_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46189.1"
                     /translation="MGKRSLVLLVLLLVGSMCLAAAAESGELILPDFLAGKVPAFVDD
                     FDNLDNWIEEEDFEFNQWAITTKGFLKVQDGCDNFC"
     gene            complement(348..1550)
                     /locus_tag="PLW82_02575"
     CDS             complement(348..1550)
                     /locus_tag="PLW82_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46190.1"
                     /translation="MKKVCTILCIISILSLGVFAKSVWELGLGLTEIEDTYYGSFSFR
                     PDIKVGKLGLGLSLTLHFNAQGLRLEDWQDNGNFSITKSLARVVRYVSWAELGDPVYF
                     RLGELSNVYVANGLIMDGYRNLSAYEIRSDIRKLGVDLAGDAGILGAHVMVNDALKPE
                     LFVLHPYVRPLYFVDGIPGFLKNTSVGVVYARDTRPAYKMPYAYGLDLTMPLLRNFVF
                     YFHTAELANNSRGHAVGARSNLGPLYLRGEYRVYGEDFRPAVYDWQYEDYGTAMVLQS
                     PKANGYMAEAGFSLLQDNLYFNIRYDNMMIEGQDSVPTLTGRAVIGSDLFAAVTGRKG
                     QLEASYSQLNFVKIGDFTNQRTKIDAAVSLELYRGLMGRYVYTVTFTENSTPVRFQAF
                     EVVLGGGF"
     gene            complement(1980..2552)
                     /locus_tag="PLW82_02580"
     CDS             complement(1980..2552)
                     /locus_tag="PLW82_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009590461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOJ46191.1"
                     /translation="MDKTIPVGVSARHVHLSQKDLEILFGEGYQLTPKKPLSQPGQYA
                     AEETVEVVGPKRSFPTVRILGPVRGQTQVELSLTDCFTIGVTAPVRESGDIKGSGSVT
                     LVGPKGQVTLEEGVIVAGRHIHMHPDDAKRFGVTDKQRVRVKTEGERAMIMENVIVRV
                     RDDFALEMHIDTDEANAAGLANGATVTIID"
     gene            2728..3075
                     /locus_tag="PLW82_02585"
     CDS             2728..3075
                     /locus_tag="PLW82_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46192.1"
                     /translation="MSLIVGDYLGTREDIEAKIGTFPHRIEPVGLRTPDQVPPGYDPL
                     GFARLVGSLYLTMPAIGANMIGSTTVDRSLATDPPEKPIGRFHYAVKVPRERLDEFVR
                     FFEERGEVHLIRY"
     gene            complement(3065..3409)
                     /locus_tag="PLW82_02590"
     CDS             complement(3065..3409)
                     /locus_tag="PLW82_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HOJ46193.1"
                     /translation="MRQAYSILWGLFAALVLYLASSAVLGLVSYLGWFELGKLGYDIL
                     HYLIVAGACFVAARKAADRGWLVGLLISLCIALFALLMQYSLGRPIMDQLVRSAITVA
                     VGTIAGALGVNI"
     gene            complement(3458..4297)
                     /locus_tag="PLW82_02595"
     CDS             complement(3458..4297)
                     /locus_tag="PLW82_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOJ46194.1"
                     /translation="MVRNLLVIALVLLMSFAAVAEERYLEFTHYSEVLGKEKKFAVIL
                     PPDYTTEKAYPVLYMLHGAGGDYLDWHKPDGKLYELSRNYQMIIVTPDVETSWLVDSH
                     IKPESKYETYMIQEFLPYIEANFSIIDSYRARGIMGLSMGGHGAMMLALKYPDIFGVA
                     SSTSGVLDIRMLPNTAGKKDVLGEPSQYPEEWNNASANYLAQNLRNMRRKPRIMFDVG
                     LGDYVYDTNMAFHKTLKEQGVDHIFRLYPGTHSWGYWVARLPEHLAFHAENLRSPQIV
                     WDE"
     gene            complement(4529..>4862)
                     /gene="fusA"
                     /locus_tag="PLW82_02600"
     CDS             complement(4529..>4862)
                     /gene="fusA"
                     /locus_tag="PLW82_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779226.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ46195.1"
                     /translation="MAFKKGFMEARPIILEPIMSVEVTVPDEYMGDVMGNLNKKRGKI
                     LGMDPGDGTQVVKALVPMSEMFRFAIELRAMTQGWGTFVSTFDHYEELPAQLAEKVIA
                     EKQQSEGN"
BASE COUNT         1227 a         1316 c         1107 g         1212 t
ORIGIN      
        1 tgcagaaatt atcacatcca tcttggacct tcaagaaacc tttggtggtg attgcccact
       61 ggttgaactc gaagtcctct tcctcgatcc agttgtcgag gttgtcgaaa tcgtccacaa
      121 aagccggcac cttgccagca aggaaatccg gaagaatcag ctcaccggac tcggctgcag
      181 cagccaagca catactccca acgagaagca gtaccaacag caccaaactt cttttgccca
      241 ccaaaaaacc ccctttatag aatgttgagt catttctggc taagttagac ttcgttgtca
      301 aaactctaac tcctttagcc tgtgtaaaat aaagggggga tgcctgtcta gaatcctccg
      361 cctagaacca cttcaaaggc ttggaatctg acaggggtgc tgttctcggt gaaggtaacg
      421 gtgtacacat agcggcccat gagcccacgg tagagctcta agctaaccgc tgcgtcgatc
      481 ttcgttctct ggttggtaaa atcaccgatc ttcacgaagt tcagctggct gtagcttgcc
      541 tccagttgcc ccttgcgtcc ggtaacggca gcaaacaggt cagatccgat gacggcacgt
      601 cctgtaaggg ttggtaccga atcctgccct tcgatcatca tgttgtcgta gcggatgttg
      661 aagtacaagt tgtcctgcag taacgagaaa ccggcttcag ccatatagcc atttgccttt
      721 ggcgactgga gcaccattgc tgttccgtag tcctcgtact gccagtcgta aactgcaggt
      781 ctgaagtcct cgccgtaaac tctgtattct ccacgcagat agagaggtcc aaggttggat
      841 ctagctccga cagcatgtcc acgggagttg tttgccagtt ccgcagtgtg gaagtaaaag
      901 acaaagttcc ttaaaagcgg catggtcaaa tccaaaccgt aagcatacgg cattttgtaa
      961 gctggtctgg tatctctggc gtagaccaca cctacagaag tgttcttcaa aaagccggga
     1021 atgccgtcaa caaaatacag gggtctgacg taaggatgaa gcacgaacag ttccggcttc
     1081 agagcatcgt ttaccattac atgagctccg aggatgcccg catctcccgc caggtcaacg
     1141 cctagcttcc tgatatcgct tctaatctcg taggcggaca gattgcggta accgtccatg
     1201 atcaaaccgt tagccacata aacattgctg agctctccca acctgaagta cacgggatca
     1261 ccgagctctg cccaggaaac atagcgcact actctggcaa gtgatttggt gatgctgaag
     1321 ttaccattat cctgccagtc ctccaggcgt aatccctgag cattgaaatg caacgtgagc
     1381 gaaagcccga gaccgagttt gccgacttta atgtccggac ggaagctgaa cgaaccgtag
     1441 taagtatcct caatctcagt gagaccgaga cccaattccc aaacggattt agcaaaaacg
     1501 cctaagctca agatactaat gatgcaaagt atggtgcaga ccttcttcac tgatagcccc
     1561 cccccttaag ttcgcgtgca gttgttttta ctattggcta attgaaacta taataccttc
     1621 ttcctctaac tttcaaactt gtctttattt gccacttgag acctaaagac tacctccggg
     1681 ttactggcgt tttaaacaaa cataagtagg gcaacgccct acttatgttt gatctagact
     1741 agtcagctga ctatcaaaaa tatccagttt gcactgatcc tcttcgatgg accagtgttt
     1801 acagtagcta caactggcat cttctgcccc ttgggtttct atgaaacccc tcagcatctg
     1861 catgcttggt gcatactcac tgcaggttga tgcaactgca atgcgctgct gctcactaga
     1921 caataagaca cccctgcagc tattatttcc ctttcttcac ggaaaaaatc aactgagcct
     1981 tagtcgataa ttgtcaccgt tgcgccgttg gccagtcctg cagcgttagc ttcgtcagtg
     2041 tcgatgtgca tctccagtgc gaagtcatca cgtacgcgga cgattacgtt ttccatgatc
     2101 atggcacgtt cgccttcagt tttcacgcgg actctctgct tgtctgtgac accgaagcgt
     2161 ttggcatcgt cgggatgcat gtgaatatgc cttcctgcaa caataactcc ctcttcgagg
     2221 gttacctggc ctttaggacc aaccagagta acgcttccag agcccttaat gtctcccgac
     2281 tctctgacag gagcggtgac gccgatcgtg aagcagtcgg tcaaagacag ctctacctgg
     2341 gtttggcctc taacaggacc taagatccta actgtaggga agctccgctt gggacctaca
     2401 acttccactg tctcttcagc agcatactga cctggctgtg aaagcggctt tttgggagta
     2461 agctggtagc cctctccaaa aagaatctcc aagtccttct gtgataaatg aacatggcga
     2521 gcactgacgc ctactggaat agttttatcc attggttttc gtcctttcat ctatagtaaa
     2581 tatcttaaaa tgcggcaaac tctcaaagtc tataagagag atttttccaa taccacaaac
     2641 ttttattcta ggtttttcat caggtctcct gcctcacgca tgcaagaatg ctcctgaata
     2701 tgcaacacta ctactggggg tgagaaattg agtttgattg tcggtgacta cttgggcacc
     2761 cgtgaagata tcgaagctaa gatcgggact ttccctcaca ggatagagcc ggtaggcctc
     2821 agaacgccgg atcaagtacc accaggttac gatcccttag gatttgctag actggttggc
     2881 agtctatacc tgaccatgcc tgcaattggg gccaacatga tcggcagcac tactgttgat
     2941 cgcagtctag ctacagatcc cccggaaaaa ccgattgggc gctttcacta cgccgtaaaa
     3001 gttcccaggg aaaggctgga tgagttcgtc aggttctttg aagaacgggg tgaagtacac
     3061 ctgatcagat attgacgccc aaggcacctg ctatggtacc caccgccact gtaatggcag
     3121 aacgcaccag ctgatccatg ataggtcttc ccaggctgta ctgcatcaac agtgcaaaca
     3181 aagcgatgca aagagatatc agcaatccta caagccagcc cctgtcagca gctttccttg
     3241 ctgccacaaa acaagctccg gcaacgataa ggtagtgcag aatgtcataa cccagcttcc
     3301 ccagttcgaa ccagcccagg taagagacca gccccaaaac cgcagacgaa gctaagtaaa
     3361 gcacaagagc ggcaaaaagt ccccacaaaa tagagtaggc ttggcgcata aatccacctc
     3421 ctaaggtaga tttattacgc caacctacta attattatta ttcgtcccaa acaatttgcg
     3481 gcgatctgag gttttctgcg tggaaggcca ggtgttccgg gagccttgcc acccagtatc
     3541 cccaagagtg ggtcccggga tagagccgga aaatgtgatc tacaccctgc tctttcaggg
     3601 tcttgtggaa agccatattg gtatcgtaga cgtagtcgcc gaggccaaca tcgaacatga
     3661 tccgcggctt gcggcgcatg ttgcgcagat tctgcgctag gtagttagca gaagcgttgt
     3721 tccactcctc tggatactgt gacggctcgc ccaaaacatc cttctttcct gcggtattag
     3781 gcagcatgcg gatgtccaag actccgctcg tagaactggc aacaccgaag atgtccgggt
     3841 actttaaggc cagcatcatt gcaccgtgtc cgcccatgga aaggcccata atgccccgtg
     3901 ccctgtagga gtcgatgata gagaagttag cttcaatgta cggcaggaac tcctggatca
     3961 tgtaggtttc gtacttagat tcgggcttaa tgtggctgtc aaccagccaa gaggtctcta
     4021 catccggcgt aacgataatc atctggtagt ttctcgagag ctcgtagagc ttcccatcag
     4081 gcttgtgcca gtccagatag tcgccaccgg caccgtgaag catgtataga acaggataag
     4141 ctttttccgt tgtgtagtcc ggcggcagaa tcaccgcaaa cttcttctcc ttacccaaaa
     4201 cctcagagta gtgggtgaat tcgaggtagc gctcttctgc aactgcagca aaactcataa
     4261 gcagcactag cgcaatcaca agcaagtttc tgaccacctt ttatcccctt cccttttgtt
     4321 tttaggcttc agatcctaga tcttatttga tgtctgtccc aaagccaaac cgggctttcg
     4381 gcccaccaaa tacatataac ccatttaagc tggagccaaa ccccgaaact ccgctggcac
     4441 ggaacgtaaa ccgtaccttc tccagggcac ctccgctcag aaaaaactgc tgcctgtggc
     4501 agcagtttcc cttctatctc gcttctcgtt agttgccctc gctctgctgt ttttcagcaa
     4561 tgaccttttc tgccagctga gccgggagct cctcgtaatg atcgaaggta cttacgaagg
     4621 ttccccaacc ctgagtcatg gctctcagtt cgattgcgaa cctgaacatc tcgctcatcg
     4681 gcaccagagc ttttaccacc tgggtgccat ctcccggatc cataccgagg atcttgccgc
     4741 gcttcttgtt caagttgccc ataacatcgc ccatgtactc atcgggtacc gtaacttcaa
     4801 cagacataat cggctcgagg atgatcggtc tggcttccat aaagcccttc ttaaaggcca
     4861 tg
//
LOCUS       DAOSDA010000073         4854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_10991_length_4854_cov_3.020421, whole genome shotgun sequence.
ACCESSION   DAOSDA010000073 DAOSDA010000000
VERSION     DAOSDA010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10991_length_4854_cov_3.020421"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PLW82_02605"
     CDS             <1..628
                     /locus_tag="PLW82_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ46196.1"
                     /translation="DFIAKCAASGVKVAIGHTAANTHEIQAAAEAGASLSTHLGNGAH
                     PVLPRHPNYIWDQLAEDRLWLTLIADGFHLPPAVIKVFLEVKKEKAILVSDAVSFAGC
                     KPGVYESLIGGKVELTEEGRLQMVSDPRLLAGSAATLLDTVEFMARTGLSTFEQAWEM
                     ASLRPYRFLDEESFGIEEGAPADLVLGRFCDGRFRVQTVIKNGEVIDL"
     gene            787..1482
                     /locus_tag="PLW82_02610"
     CDS             787..1482
                     /locus_tag="PLW82_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HOJ46197.1"
                     /translation="MLISKKDLLAETGISYGQLYRWKRQGLIPDEWFIKQSTFTGQET
                     FFPKKQILARINAIQRLKDQYSLEELAKMLSPEISERMFSHRDLEIIEEVENGLIPLF
                     FQVFEKKSFSFIEVLFLIAISTFYKKHDLELVDVENLCLGAKDYLDEIKQTDFLCLLL
                     SHDGGYFTVIQDENVPVFIDKRIKKVDEIRLNEVSSNMKLKYRKSFNFEFDEERETNE
                     QKITLMKPGEVLT"
     gene            1479..2312
                     /locus_tag="PLW82_02615"
     CDS             1479..2312
                     /locus_tag="PLW82_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46198.1"
                     /translation="MTIALGDKYFEGTYKRIFAAGDIELVNSKIGSIYAAGDVDIKRS
                     EVKKVRAAGDVNGEEVTLGSAKVVGDIELKGVCTAKDFVAFGDVSAEYLECKCLRFGF
                     PKAKTNNAPSLSGFYKAETFELVMPLTLSCEYEFQNIIATGEMRAKAEIVCTNFYGLA
                     KIFAPAVNAEEVFILTSDGTEIGEVAGTKVTVSKRFEPDRAFRKLPKTLAVRDADSRG
                     AIIKIGSIEGDKVSVENVCADSISGIDVEIGDLCVVEEVAYKNSIRISEKAVVGRIVR
                     L"
     gene            2309..3460
                     /locus_tag="PLW82_02620"
     CDS             2309..3460
                     /locus_tag="PLW82_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOJ46199.1"
                     /translation="MRKERIEGIGSIVGGEYESIEAEGLAKLKGDVKAETFTIEGLFK
                     SKGRIEAKTLKIEGVHRAFRDIKAEEVIIDGILKLKRASLMADRIKCDGVITCTEEIN
                     TDKFDFEGVCSIASLVGDQIKIRYARSSGDKISIDGKFARLISSYFGRKLDAEKCIVD
                     SVECTSLTASGTKFKTVRAHSVQLSDYCEVDVLYVDGEAEIDSTCVVRKIIKESAQVI
                     VKEEPKVASPTLTKIMDLYKEGKINADEAEKMIEALKELPKGGGGSLGRMVAGGLPWA
                     DDGKLRVVAFLGHELLEKSAPESKTIEVTYNGEAVDVDVYGSLSCGDVEGNVSAGGSV
                     KCSNVGGDVKAGGSITCGNIDGNVNAGGSVTCSEVTGEIRAGGGVRVGK"
     gene            3420..>4854
                     /locus_tag="PLW82_02625"
     CDS             3420..>4854
                     /locus_tag="PLW82_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46200.1"
                     /translation="MRLEPVVESEWESEGLLVEASLVQNRKYKFYDFVLTPLKVSPIL
                     TLIKIFNQVLLSLIPALRILVTADFIDTAMKIFSGEAARVEIYRPLVYFMLFIAYANL
                     NNHLMNFVNLKLEMNLNRAYRSQIAEKRAKLEYRHIEDNDTWDLISRTCQDPVGRILG
                     GFNNLLSAAGIVIQVFSILSVLVAQDPWAGVTIVLISVPLFALAIKGGKATYKASVEA
                     EKHMRRANYLQSLLQSRENVEERALFGYVDSVNEKWLGKYEESRIINTKVRLKYFIRM
                     KGSSLITVFLSIAITGVLLAPLSQGIITVGMFMGLVIGTLNLIQMMSWQLSWTMSELA
                     RNREYLKDLTKFSELSETDGALELPVDKNSVVFENIEFRNVSFKYPGTDRYILKNFNL
                     RLEKDLHYAFVGINGAGKTTVTKLITGLYDNYEGEILLNGRELRTYSQAELKALFAVV
                     YQDFAKYYIPLKDNIALGNVLNFNDQEV"
BASE COUNT         1313 a         1049 c         1350 g         1142 t
ORIGIN      
        1 ggatttcatc gccaagtgcg cagctagcgg agttaaagtt gcaattggcc acaccgcagc
       61 aaacacccat gagatacagg ctgcagcaga ggccggggct tccctttcca cccatctagg
      121 aaacggcgct cacccggttc tgcccagaca ccccaactac atctgggacc agctggcaga
      181 agatcggctc tggctgacac tgatcgccga tggattccat ctaccgcctg cggtaatcaa
      241 ggttttcctg gaagtgaaaa aggagaaagc gattttggtt agcgatgctg tgagctttgc
      301 cggatgcaag cctggagtgt acgaaagcct aatcggaggg aaggtcgagc tcacagagga
      361 ggggaggctc cagatggtgt cggatccaag gctccttgca ggatccgctg caacgctcct
      421 ggacactgtg gagtttatgg caaggaccgg tctgtccact ttcgagcaag cctgggagat
      481 ggcaagcctc cgaccgtacc gcttcttgga cgaagagtct ttcggcatcg aggaaggagc
      541 gccagcagat ctcgtcctcg ggaggttttg cgacgggaga ttccgtgttc aaacagtgat
      601 caaaaacgga gaagtgatag atctttagaa cgcccgacat cgggcgtttt tccttttaaa
      661 ggtgcgtatt cgttgccaat agaggtttgg gagaattttg caaaaagttt aaaatgaccc
      721 ttgcctagcg taacaatgtt atgttatagt gaacgtaaca aaacattgtt acgttaggtg
      781 agtgctatgt taatttctaa aaaagacttg ttggccgaaa cggggatctc ttacggtcag
      841 ctgtaccggt ggaagaggca ggggctgata cccgatgagt ggttcatcaa gcagtcgact
      901 ttcaccggtc aggagacctt tttccccaag aagcagatac tcgcgcgcat caacgccatt
      961 cagcgtctga aggatcagta ttcgttagaa gagctggcca agatgctgtc gccggagatc
     1021 tcggaaagga tgttttccca cagggatctg gagatcattg aagaggtaga aaacggcctg
     1081 ataccgctgt ttttccaggt gtttgagaaa aagagtttca gctttatcga ggttctcttt
     1141 ctcatcgcta tctccacctt ctacaaaaag cacgatctag agcttgtgga tgtggagaac
     1201 ctctgccttg gtgccaagga ctacctagac gaaatcaagc agacagactt tttgtgtctc
     1261 ctgctcagcc acgatggcgg ctacttcact gtaattcagg atgaaaacgt accagtcttt
     1321 atcgataagc ggatcaagaa ggttgacgag attcgcctga acgaagtgtc cagcaacatg
     1381 aagctgaagt acagaaagag cttcaacttc gagttcgacg aagagcggga aacaaacgaa
     1441 cagaagataa ccctcatgaa gcccggggag gttttaacat gaccattgca cttggagata
     1501 agtactttga aggcacctac aagcgtatct ttgctgcagg ggacatcgaa cttgtaaaca
     1561 gcaaaatagg ttcgatctat gctgcaggag atgtggatat caaacggtca gaagtgaaaa
     1621 aggtccgggc tgcaggcgac gttaacgggg aagaagtgac gcttggttcg gccaaagttg
     1681 taggcgatat agagcttaaa ggagtctgca cagccaaaga ctttgtggct tttggggacg
     1741 tgtctgcaga atatctggag tgcaagtgcc ttaggttcgg gtttcccaaa gctaaaacca
     1801 ataatgcgcc tagcctttcc ggtttttaca aggccgaaac gttcgaactt gtgatgcctt
     1861 tgacgctgtc ctgcgagtac gagtttcaaa acatcatcgc caccggagag atgcgggcaa
     1921 aggcggaaat cgtttgcacc aacttctacg gtctggcaaa gatttttgct ccagctgtta
     1981 acgcagaaga ggtgttcatc ttgacttcag acgggaccga gatcggcgaa gttgcgggca
     2041 ctaaagttac cgtatccaag cggtttgagc ccgatcgggc ttttaggaag ctaccgaaga
     2101 ctttggccgt tcgagatgcc gattccagag gagcaattat taagatcggt tctattgaag
     2161 gggacaaagt gtccgtagaa aacgtgtgtg ctgattcgat ttccggtata gacgtggaga
     2221 ttggcgacct gtgcgtcgtg gaagaggtcg catacaaaaa ttccatcagg atttcagaga
     2281 aagcggttgt tgggaggatc gtgagactat gagaaaagag cgaattgagg ggatcggttc
     2341 gattgtcggc ggtgagtatg aatccatcga ggctgaaggt cttgccaaat tgaagggcga
     2401 tgtgaaagcc gagaccttca ctatcgaggg gctctttaag tcgaaagggc ggatcgaagc
     2461 taaaaccctg aagatcgaag gggtgcacag ggcctttaga gacatcaagg ctgaagaggt
     2521 tatcattgac gggattttga agctgaaacg ggcatctctc atggcagata gaatcaagtg
     2581 cgacggcgtg atcacctgca ccgaagagat caataccgac aagttcgact ttgagggagt
     2641 ctgttccatt gcatctttgg taggcgacca gataaagatc cgctatgcga gaagttcagg
     2701 ggataagatt tccatcgatg ggaagtttgc cagattgata agctcctact ttggcaggaa
     2761 gctagatgcc gagaagtgca ttgtggacag cgtagagtgc acgtcactca ccgcatctgg
     2821 aacaaagttc aagactgtta gagcgcacag cgtacagctt tccgactact gcgaagtgga
     2881 tgtgctctac gtcgacggtg aagctgaaat cgacagcacc tgtgtggtgc ggaagatcat
     2941 aaaagaaagc gcccaagtga tagtaaagga ggaaccgaag gtggcaagtc caaccctcac
     3001 aaagatcatg gatctttaca aagaaggtaa gatcaacgct gatgaagcag agaagatgat
     3061 cgaagcactg aaggaactgc caaaaggagg aggcggttct cttggacgta tggtagcagg
     3121 aggtctgcct tgggccgacg acggcaagct ccgggttgtg gcctttttgg gacacgagct
     3181 tctggagaaa tccgctccgg agagcaaaac cattgaggta acctacaatg gagaagctgt
     3241 cgatgtagat gtctacggca gtctcagctg cggagacgtt gaagggaacg tgtccgcagg
     3301 agggtcggtc aaatgcagca atgttggcgg agatgttaaa gctggaggta gtattacctg
     3361 tggcaatata gatgggaatg tgaatgctgg aggtagcgtc acctgttcag aagtgacagg
     3421 tgagattaga gccggtggtg gagtcagagt gggaaagtga gggtttacta gtggaagcaa
     3481 gtcttgttca aaatcgcaaa tacaagtttt acgacttcgt tcttactccc ttgaaggtaa
     3541 gtcctattct cacgttgatc aagatcttta accaggtttt gctctcgctc attcctgcgc
     3601 tgagaatctt ggtaactgca gatttcatcg acacggctat gaagattttc agtggagaag
     3661 ccgccagggt cgagatctac aggccacttg tctacttcat gctgtttatc gcctacgcca
     3721 atctcaacaa ccatctgatg aattttgtca acctgaagtt ggagatgaac cttaacaggg
     3781 cttacagaag ccaaatagcg gagaaacggg caaagctcga gtaccggcac atcgaagaca
     3841 acgacacctg ggatcttata agccgcacct gtcaggaccc ggtaggcagg atccttggag
     3901 ggttcaataa tctgctcagt gctgcgggta ttgtgattca ggtcttttcg attctgtctg
     3961 tactggtggc ccaggatccg tgggcgggag tcactatcgt tctgatctcg gtgcctcttt
     4021 ttgctctggc cattaaaggc ggaaaggcca cctacaaagc cagcgttgaa gcagagaagc
     4081 acatgaggag agccaactac cttcaaagcc ttttgcagag cagagaaaac gtggaagaac
     4141 gggctctgtt tggctacgtt gacagtgtca acgagaagtg gctggggaag tacgaagagt
     4201 cgagaatcat caacactaaa gtccgtctta aatacttcat cagaatgaaa ggctctagtc
     4261 tcatcacggt ctttctttca attgcgatta ccggcgtgct ccttgcacct ctttcacaag
     4321 gaatcatcac cgtcggtatg tttatggggc ttgtgatagg aacgctgaac ctcatccaga
     4381 tgatgagttg gcagctctcc tggaccatga gcgagctggc cagaaaccgc gaatacctta
     4441 aggatctcac caagttttcc gagctgtcgg agaccgacgg tgcgctggaa cttcctgtag
     4501 acaaaaattc cgtggttttc gagaacatcg agtttaggaa cgtttccttt aaatacccgg
     4561 gaaccgatag atacattttg aagaacttta acttgaggct ggaaaaggac ctgcattacg
     4621 ctttcgtggg aattaacggc gcaggcaaaa ctacggtgac aaagcttatt accggtctgt
     4681 acgacaacta tgaaggggag attctcttaa acggtagaga gctgagaacc tacagccagg
     4741 ctgagctgaa ggcgttattt gccgtagttt atcaggactt tgccaaatac tacattccgc
     4801 tcaaagacaa catcgcctta ggaaatgtgc tgaacttcaa tgatcaggaa gtgc
//
LOCUS       DAOSDA010000074         4823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11095_length_4823_cov_2.836409, whole genome shotgun sequence.
ACCESSION   DAOSDA010000074 DAOSDA010000000
VERSION     DAOSDA010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11095_length_4823_cov_2.836409"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..775)
                     /locus_tag="PLW82_02630"
     CDS             complement(53..775)
                     /locus_tag="PLW82_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46201.1"
                     /translation="MKRFLVAVLVVAFLALAVSAVDNRFVRDDIPLKRLVGPGGIYMQ
                     LSQYNSNYSIPSIVGYEVELAVFTPEFEDQIRTVPVAFMKVFVVIDDVITEILNRVPK
                     IPENGYLVVGHGRAGSVFMEQFKVGDRVKVEDYEPVYDFAEYPKFVVLPDGTKLEIDA
                     WNRHRDTDEIIIYNTDFADRTYTNEWGIEIAVERGEVVEIRKQLNTEPLDIPRKGFVI
                     SVHGKNIPYFADIVEYDYIELE"
     gene            complement(923..1936)
                     /locus_tag="PLW82_02635"
     CDS             complement(923..1936)
                     /locus_tag="PLW82_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOJ46202.1"
                     /translation="MLQKLTAVPSPTGYTKQISAYLVEVLKDLGYSPEVSNKGAVLCP
                     VGGEGSGLLLSAHVDTLGLMVRALKSNGRLRFTKIGGFHEATIENENCVVHTRCGKSY
                     SGTVQLLEPATHVNRELGKQERSDETMEIVIDEKVSSKEDLEKLGISAGDFISLDPRT
                     TVTPSGYIKSRHLDDKASCAVLLTLAKYLKDQGVTPKRAVYLLFTTYEEVGHGASSFV
                     PGDVVDFVSVDMGAVGDDLTTDECKVSICAKDSMGPYDYDLTGDLISTAKRLGLNFAV
                     DVYPNYGSDAGAALRAGFDVRHGLVGPGVSASHGYERTHREGLENTLKLLLGYVLQAG
                     EVK"
     gene            complement(1998..3491)
                     /gene="glpK"
                     /locus_tag="PLW82_02640"
     CDS             complement(1998..3491)
                     /gene="glpK"
                     /locus_tag="PLW82_02640"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOJ46203.1"
                     /translation="MAQYILALDQGTTSSRAIVFDEGGRIVSEARKEFTQIYPKPGWV
                     EHNAVEIWGTQIGVAYEAIAKANTAPSQLQGIGITNQRETTVVWERSTGRPIANAIVW
                     QCRRTAAICEELKKQGLSEIVRRKTGLEIDAYFSATKIKWLLENIPGAREKAQKGELC
                     FGTIDTWLIWNLTKGRVFVTDYSNASRTMLFNIHTLSWDEELCEIFGVPMSLLPEVKP
                     SSYIYGETDSSILGAAIPIAGCAGDQQAALFGQCCTEPGEAKNTYGTGCFMLMNTGDK
                     AIESQNGLLTTIAWKLGDQVTYALEGSIFIGGAAVKWLRDELRMIKTAAETEDYALEV
                     EDTGGVYFVPAFVGLGAPHWDMYARGTMVGLTRGTRKEHFIRAVLESIAYQSYEVVKA
                     MEEDSKLKLKALRVDGGASANNFLMQFQADILNVPVQRPKVLEITALGVAYLAGLATG
                     YWQNTGEIRNKCGCHEDIVPSMEEDRRAQLIKGWKKAVERAKGWAEE"
     gene            complement(3533..4093)
                     /locus_tag="PLW82_02645"
     CDS             complement(3533..4093)
                     /locus_tag="PLW82_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46204.1"
                     /translation="MVKDSRLYSSEIVSKVRLNAAKMEQYRDGRVFNEIELVLGFSSY
                     VYWLAALFRLDEKGQDDFLSRASETAREIALDFVAMLKAKPELLEAVQKVTPFNPDIE
                     LVWGREKATHNHWKFSLTRAIILITRQLVYATVDFIASDEKEASADSLYFLAFSELAD
                     LFQEAVVWASYVIGVEHAQELFNLEG"
     gene            complement(4109..4735)
                     /locus_tag="PLW82_02650"
     CDS             complement(4109..4735)
                     /locus_tag="PLW82_02650"
                     /inference="COORDINATES: protein motif:HMM:NF039337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HOJ46205.1"
                     /translation="MNLYRLKLSEMIDGMKAGECPFCFIDALFYKWYFKRLIEGVLDE
                     DYKEEWTKGGGFCPEHTKMLLKQGSATGHAILYQYLLEDWKERWTQGDFDCPVCAKIA
                     KDLADSKKVFAWGLKNDAEFLEEYKKSSGICRGHFVSVYGELDEDLKKLVVEVQERAF
                     ERLKENLELLLRQHDYNYDGPKNAESLRSWKKAARYYIGGLDKQYKAR"
BASE COUNT         1068 a         1374 c         1094 g         1287 t
ORIGIN      
        1 cttttcgcga aagccaactt gcaactatct gagaacgtcc ctctttttga ctttactcaa
       61 gttcgatgta atcgtattcc acaatgtctg caaagtatgg gatgttcttg ccgtgtaccg
      121 agatgacaaa accctttcgg gggatatcga gaggttcggt gttcagctgc tttctaatct
      181 cgacaacttc tcctctttct accgcaatct caatgcccca ttcgttggtg taggttctgt
      241 cggcaaagtc ggtgttgtag atgatgatct catctgtgtc gcggtgcctg ttccaggcat
      301 cgatctccag tttagttccg tcgggcagaa cgacaaactt cggatactca gcaaaatcgt
      361 agaccggttc gtagtcttcg accttaactc tgtcgcctac tttgaactgc tccataaaga
      421 ccgaacctgc ccggccgtgc cctactacca gatagccatt ttcagggatt tttggcactc
      481 tgttgaggat ctcggtgatc acatcatcga tcacgacaaa caccttcatg aacgcaacag
      541 gcacagtcct gatctgatcc tcaaactcag gcgtaaacac cgcaagctcc acttcgtacc
      601 cgacaatgct gggaatggag tagttgctgt tgtactggga aagctgcatg tagattccgc
      661 cggggcctac cagtcgcttc agtgggatgt cgtctctgac aaacctgttg tccaccgctg
      721 ataccgctaa cgccaagaac gccacaacta gcactgcaac gagaaaacgc ttcataagct
      781 tgcgcaatac tgcgcttccc tccttccaaa gtgcccttgc tccgggtttt gggctcaata
      841 cccaacttca gcggcgccgg tctttttcct tttcctgacc tacctactca cgtactccct
      901 ccaacctgct gaaaacgcta aactacttaa cttctccggc ctgcagaaca tacccaagaa
      961 ggagctttag agtgttttcc agcccttccc tgtgagtacg ctcgtacccg tgagacgcgc
     1021 tcaccccggg cccaacaagc ccgtggcgga catcaaaacc cgctctgagg gcagcacctg
     1081 cgtcagagcc gtagtttgga taaacgtcca ctgcgaagtt cagtcccaac ctttttgccg
     1141 tgctaatcaa atcgccggtc aggtcgtagt cgtacggtcc catggaatct ttggcgcaaa
     1201 tggagacttt gcattcatct gttgtcaggt catcgcctac tgcacccata tccaccgaga
     1261 cgaagtcgac cacatcacca ggtacaaacg atgaagcccc gtggcctact tcttcgtagg
     1321 tggtaaacaa aagatagacc gctctctttg gcgtaacgcc ttggtctttg agatactttg
     1381 ctagggtaag caaaacagcg cagcttgcct tgtcgtccaa atgacggctt ttgatgtaac
     1441 ccgacggcgt cactgtggtt ctaggatcca aagagatgaa gtctcctgcg ctgatgccca
     1501 atttttccag atcttccttg gaggagacct tttcgtctat aacgatttcc atggtctcgt
     1561 cgctacgctc ctgcttgccc aactctcggt tgacgtgggt tgcaggctcc aaaagctgca
     1621 ccgttcccga gtaggacttg ccgcatctgg tatgcactac gcagttttcg ttttcaatag
     1681 ttgcctcgtg aaatcccccg attttggtga aacgcagcct gccgttgctt ttcagagctc
     1741 tcaccatgag ccccaaagtg tccacatgag ccgaaagcag aagtccgctt ccctctccac
     1801 ctaccggaca gagtactgct cctttgttgc tcacctcagg gctgtacccg aggtctttca
     1861 acacctctac taagtacgcc gagatctgtt ttgtatagcc cgtagggctg ggaacagccg
     1921 taagcttctg cagcatctct acagtatagt taagataact ctgatctaac tttaaagcat
     1981 ccactctaat ccactcctta ctcctctgcc caacctttag ccctttccac ggcctttttc
     2041 cagcccttga tcagctgggc tcgtctatct tcttccatac tcggtacaat gtcctcgtgg
     2101 catccgcatt tgttcctgat ctctccggtg ttctgccaat agcctgtggc aaggccggca
     2161 agatacgcca ctcccaaagc agtgatctcc aggactttag gcctttgcac cggcacgttt
     2221 aagatgtccg cttggaactg cataaggaag ttgttagcgc ttgctccccc gtccactctg
     2281 agggctttga gcttcagctt agaatcttcc tccatggctt tgacgacttc gtaggactgg
     2341 taggctatgg actccagaac tgcacggata aagtgttctt ttctcgttcc tctggtcagc
     2401 cctaccattg tccctctggc gtacatgtcc caatggggag ctcccaagcc cacaaaagcc
     2461 ggaacaaagt agacgccacc ggtatcctct acctccaggg cgtagtcctc tgtttctgct
     2521 gcggttttta tcatccgcag ctcatctctc aaccacttca ccgcagcgcc gccgatgaaa
     2581 atgcttcctt ccagagcata ggtcacctgg tcccctagct tccaagcgat ggttgtaaga
     2641 agcccgtttt gcgattcaat agctttgtca ccggtgttca tgagcatgaa gcaaccggtg
     2701 ccataggtgt tcttggcttc ccccggctcg gtgcagcact gcccgaataa cgctgcctgc
     2761 tggtcgcctg cacagcctgc gatgggaata gcagctccca aaatgctgga gtcggtttcg
     2821 ccgtagatgt aagaagaagg ctttacctcg ggcagaagtg acatgggaac gccgaagatc
     2881 tcgcagagct cctcatccca cgacagagtg tggatattga acagcatggt tctcgatgcg
     2941 ttggagtagt cggtgacaaa aaccctgcct ttggtaagat tccagattag ccacgtgtcg
     3001 atagtgccaa agcacagctc gcccttttgg gccttttccc gtgctccggg aatgttttcc
     3061 aggagccatt tgattttagt tgcggaaaaa taagcatcga tctccaaacc ggttttcctc
     3121 cgcactattt ccgacaagcc ctgctttttc agctcctcgc agatggcggc ggtccttctg
     3181 cactgccaga cgatggcgtt ggctataggc cggccggtgc ttctttccca tacaactgtg
     3241 gtttctctct gattggtgat accgattccc tgaagctgcg acggtgcagt gttagcttta
     3301 gcaattgcct cgtaagctac accgatctgc gtaccccaaa tctccactgc attgtgctcc
     3361 acccagccgg gtttgggata aatctgtgta aactcctttc gggcttcaga tacaatcctt
     3421 ccgccttcgt caaagacaat tgctcgagaa ctagtggttc cctgatctaa cgccagaata
     3481 tactgtgcca cccaaatccc ccttttctaa ccaacaggca gagcgcctgc gtttatcctt
     3541 caaggttgaa aagctcttgg gcgtgttcaa cgcctatgac gtagcttgcc caaaccactg
     3601 cttcctggaa cagatcagca agctcggaaa atgctaggaa atacaaactg tccgcagaag
     3661 cctccttttc gtcggaagcg atgaagtcca ccgttgcata gaccagctgt ctggtgatga
     3721 ggatgatggc gcgtgtcagc gaaaacttcc agtggttgtg cgtggctttt tccctgcccc
     3781 agaccagctc tatgtcgggg ttaaacggag taaccttttg aacggcttcg agaagttcgg
     3841 gttttgcttt gagcatggca acaaaatcca gggcaatctc ccgagcagtc tcagatgctc
     3901 tggagagaaa atcatcctgt cccttttcat ccagacggaa aagagccgcc agccagtaca
     3961 cataggatga aaaaccgaga acaagttcga tttcgttgaa aacgcgacca tctctgtact
     4021 gctccatttt agcagcattc aagcgtactt tgcttactat ttccgagctg tagaggcgtg
     4081 agtctttaac catagccgtt cctcctgctc atctagcctt gtattgtttg tcaagaccgc
     4141 cgatgtagta gcgagcggct ttcttccagg aacgcaggct ttctgcgttt ttgggtccat
     4201 cgtagttgta gtcgtgctgg cgcaggagga gttcgagatt ttcctttagt cgttcgaagg
     4261 ctctctcctg aacctccact accagtttct tcagatcctc gtctagttct ccgtaaacgc
     4321 ttacaaagtg tccgcggcag atccctgacg acttcttgta ctcctcaaga aactccgcgt
     4381 cgtttttcag cccccaggca aagacctttt tgctgtctgc aagatctttt gcgatcttag
     4441 cgcacacagg gcagtcgaaa tcgccctggg tccacctttc cttccagtct tccaaaagat
     4501 actggtacaa gatggcatga ccggtagccg atccctgctt caacagcatc ttggtgtgct
     4561 ccgggcagaa acctccgccc tttgtccatt cttccttgta gtcttcgtct aaaacacctt
     4621 cgataaggcg cttgaaatac catttgtaaa acagtgcatc gatgaagcag aaggggcatt
     4681 ccccagcttt catgccgtcg atcatttccg atagtttcaa tctatagaga ttcaacaaac
     4741 acttcctcct taagctctac cagtcatttt tgccataaca gctttagcaa taggtttgtc
     4801 tgcaggagcc aggttgtagt cca
//
LOCUS       DAOSDA010000075         4800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11169_length_4800_cov_3.090832, whole genome shotgun sequence.
ACCESSION   DAOSDA010000075 DAOSDA010000000
VERSION     DAOSDA010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11169_length_4800_cov_3.090832"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLW82_02655"
     CDS             <1..326
                     /locus_tag="PLW82_02655"
                     /inference="COORDINATES: protein motif:HMM:NF025020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HOJ46206.1"
                     /translation="LFYVKKDLPSLDGLKVSRPGWFLGQIKPMRFEPSQALIMALKAH
                     QVKRKVVLDLKDQRLNAYLKGQTFSADGPAGWVGVFLGDFPLGWGKITGGTLKNHYPK
                     GWRMT"
     gene            326..1033
                     /locus_tag="PLW82_02660"
     CDS             326..1033
                     /locus_tag="PLW82_02660"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOJ46207.1"
                     /translation="MERLDKILAHLGTGTRKEVRSLIRAGLVTVDDTVITDPGYKIDP
                     EHVKIEVDGQELAYLRHIYIMMNKPSGYITATEDRFQSTVLDLIDPSDWRRDLFPVGR
                     LDKDTEGLLLLTNDGALAHMLTAPKYKVPKKYYFETVEEIDEKRLRTLLEGVDLGDFI
                     AKATSVEKLGPKEGVLVIEEDKFHQVKRMCAALGYTIVYLERTEFGPLFLDRSLPRGG
                     YRYLTEEELASLKEAVR"
     gene            1066..1617
                     /locus_tag="PLW82_02665"
     CDS             1066..1617
                     /locus_tag="PLW82_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ46208.1"
                     /translation="MEQKDNVEQKHPDDFKDLYDRYVKKINDYLLRMSGDPLVAEELT
                     QETFYRAFTNLRSFKGDKVGAWLFRIAHNLYIDEYRQRKHFAGAEMLDIQPAIYPGDL
                     PEKHLESVETQKRVQQILQKLPENYRSILLLRDHLGLSYDDIAETIGKSSSTVKVTLF
                     RARAKFRELYTAFEQKGGNKVER"
     gene            1607..2635
                     /locus_tag="PLW82_02670"
     CDS             1607..2635
                     /locus_tag="PLW82_02670"
                     /inference="COORDINATES: protein motif:HMM:NF025164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti sigma factor C-terminal domain-containing
                     protein"
                     /protein_id="HOJ46209.1"
                     /translation="MRDKCDVVQDLLPIYLDGELREGTKRFVEEHLKECSTCRQVLED
                     SFEGEKLFREVIQDSRIDAMSARKMWYKSMLKYLVKPAFWVFMIALIGVIIFATIVSR
                     PNLLNYEILTTAASAEEMLHPGFFKASLNRESINALNFNLEKFSSPSDNIRTTYQYRV
                     SLVNDSLIFERLYSFEDSSVELYYPDVATPKPAMMDPTIVAEMLEPTLFTLSFNRFVT
                     LQELEVLFESSDIEVLWLGIASRNLHDTTKWLSFWDNWGLPVYYLQSNIQRRKNAVED
                     EMRKRVKSFTDYGKYLAGIDPYLSQRLEYVNENGIQVYGVVVRTKGPVMLDWLEKYPL
                     VRYFRRMS"
     gene            2669..3634
                     /locus_tag="PLW82_02675"
     CDS             2669..3634
                     /locus_tag="PLW82_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ46210.1"
                     /translation="MTQAEVQEVKQFCEKVKDNVEKVIIGKSKVIDQVLVALLCAGHV
                     LTEDVPGTGKTMLARSLAISLGLEFSRIQFTPDLLPSDVTGVSVFNQKTSEFEFKEGP
                     IFAQIVLADEINRATPRTQSALLECMEEKQVTTDGKTRKLPKPFLVIATQNPVEYEGT
                     FPLPEAQLDRFLMKISLGYPTENEENEILVRLQYTHPVETIESVADGSSLVRMQELVK
                     KTFVEESVREYIVRLVHATRNHSDVSIGASPRGSLALYKTAQAYATIKGREYVIPDDV
                     KYLAPLTLSHRIIIKPESSLRGKTPEKVLEEILETVPVELESLKS"
     gene            3709..>4800
                     /locus_tag="PLW82_02680"
     CDS             3709..>4800
                     /locus_tag="PLW82_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOJ46211.1"
                     /translation="MPYWRALIWIAGISALLFKGPFIYFMFYLLLLVWLIPPMLLRSS
                     EKHLRITREIETDAVFFGERMKVKIKVQNASRFPLVWLKIREACPRQIGPKMRSSVSW
                     VVSLKPGETKTLEYHIFGGKRGAYVIGPIKLELGDIFGLYKRELELQLFDFVTVYPKV
                     VPIEELGLTSTQPIGNLKHPQKIYEDPLKIAGLRDYKPGDSPKHISWKATARQEKLMV
                     REYEATMTLDTMILVDLDSRDYVSATYENAKELAISVAASITAHLTRKRQAIGLATNG
                     VPMAEASAHSDTRILDDEPLENIMPKKGQGRQFLEILSRIEGTSSGESFVGLCQRMKA
                     KLPWGTTVVMIVPRDTDELMRFAQSLIESG"
BASE COUNT         1370 a         1073 c         1219 g         1138 t
ORIGIN      
        1 atctgttcta tgtaaagaaa gatcttccgt cactagacgg gctgaaggta tcccgtccag
       61 gctggttttt aggccaaatc aaaccgatgc ggttcgaacc atctcaggcc ttgattatgg
      121 cattaaaagc ccatcaggta aagcggaagg tagtgctgga tctaaaagat cagaggttga
      181 atgcatacct caaagggcag acattcagcg cagacggtcc ggcaggctgg gttggtgtat
      241 tcttaggcga ctttccttta ggatggggaa agatcacagg aggtacactg aaaaatcact
      301 atcctaaagg atggaggatg acataatgga gcgtttggac aaaatcctcg ctcatttagg
      361 taccggtacc agaaaagagg tgcgctccct aataagagca gggcttgtaa cagttgacga
      421 taccgtgata accgatcccg gatacaagat cgatcctgaa catgttaaga tagaagttga
      481 tggccaggag ctggcctatt tgcgccacat ttacatcatg atgaacaaac cttcgggata
      541 catcaccgca actgaagatc ggtttcaaag tacggttctg gacttgatcg atccgtcaga
      601 ttggcgcaga gacctgtttc ctgtgggcag actggacaaa gatacagaag gccttctgct
      661 cctaacaaac gacggagcgc ttgcgcatat gcttacagcg cccaagtata aagtgcctaa
      721 aaagtactac ttcgaaaccg tggaggaaat cgacgaaaag cgcctgagga ctcttctcga
      781 aggcgttgat ttaggcgatt ttatcgccaa agcaacaagt gtggaaaaac tcggcccgaa
      841 agaaggagta ctggttatcg aagaagacaa gttccaccaa gtaaagcgaa tgtgtgcagc
      901 actgggatat acgattgtgt acctagagag gacagagttc ggtccgctct ttttagatag
      961 aagccttcca agaggcggat accggtacct tacagaagaa gaattggctt ctctcaaaga
     1021 agcagttagg taaattgact cagctcgggt gaagggggtg acatcatgga gcagaaggac
     1081 aacgtagaac agaaacatcc agacgatttc aaagatcttt atgaccgcta cgtcaaaaag
     1141 atcaacgatt acctgctccg gatgtcagga gaccccttgg ttgccgagga gctgacccag
     1201 gagacgttct acagggcatt taccaacctg aggtctttca aaggtgacaa agtgggcgca
     1261 tggctgtttc gcatcgccca caacctttat atcgacgaat accggcaaag gaagcacttt
     1321 gccggagcgg agatgctcga catccaacct gcaatctatc caggggatct tccggagaag
     1381 caccttgaat ctgtagagac ccaaaagcgg gttcaacaga tcctgcaaaa actgccggaa
     1441 aactacagat cgatcctgct ccttagagac cacctgggat tgtcttatga cgatatagcc
     1501 gaaaccattg gaaaatctag ttcaacggtt aaggtcacct tgtttcgtgc aagagccaag
     1561 ttcagagaac tgtacacagc ctttgaacaa aaaggaggga acaaagttga gagataagtg
     1621 tgatgttgtc caggaccttt tgccaattta cttagatgga gagcttcgcg aagggaccaa
     1681 gcgttttgta gaagaacacc ttaaagaatg cagcacatgc aggcaggtct tggaagactc
     1741 ttttgagggc gaaaagctgt tcagagaggt tattcaagac agccgcattg atgccatgtc
     1801 tgctagaaaa atgtggtaca aatcaatgct taaatatctt gtaaaacctg cattttgggt
     1861 gttcatgatt gctctcatcg gcgtgattat ctttgctacc atcgtttctc gtccaaactt
     1921 gcttaattat gaaatactga ccactgctgc atcagcagag gagatgctgc acccgggatt
     1981 tttcaaggct agccttaatc gcgaaagcat caacgctctg aacttcaacc ttgagaagtt
     2041 ctccagtcct agcgacaaca tccgcaccac ctatcagtac cgcgtaagtt tggtgaatga
     2101 ttcgctcatt tttgaaagac tgtattcgtt cgaggacagt tcagttgaac tctattaccc
     2161 cgatgtggca acgccaaagc cggctatgat ggatcccacc atcgttgcag agatgctcga
     2221 gccgactctg tttaccctca gcttcaaccg cttcgtcacg ttgcaggaac tcgaagtcct
     2281 ctttgaaagt agcgatattg aggttttgtg gttgggcatt gcatcgcgga atctccacga
     2341 taccactaag tggttgagct tttgggataa ctggggcctt cccgtctact acttacaaag
     2401 caacatccag cggcggaaaa acgcagtcga ggatgaaatg cgtaagcggg taaagtcgtt
     2461 taccgattac ggcaagtatt tggcaggaat tgatccctac ctgtcgcaaa gattagagta
     2521 tgtgaatgaa aatggcatcc aggtttatgg agtagttgtt cggaccaaag gtcctgtgat
     2581 gttggattgg ttggagaagt acccccttgt ccgctacttt aggcggatgt cttaaagtct
     2641 ttgatatgga gggataactg tggagaaaat cacccaagca gaagtgcaag aagtgaagca
     2701 gttttgcgaa aaagtgaagg acaatgtgga aaaggttatt atcggcaagt cgaaagttat
     2761 cgatcaagtt cttgttgcac ttctctgtgc gggacacgtc cttaccgaag atgttcctgg
     2821 aacaggaaag acgatgctag cacgcagcct tgccatcagc ctcgggctgg agttcagccg
     2881 cattcagttt accccagacc ttcttccatc tgacgtgact ggagtttcgg tgttcaacca
     2941 aaaaacctca gagtttgagt tcaaagaagg tccgatcttc gcacagatcg tgcttgctga
     3001 cgagatcaac cgcgccacac ccagaactca gtcggcgctt ttggagtgta tggaagagaa
     3061 gcaggtaact acagatggca agactcggaa actgccaaag ccgtttctgg ttattgcaac
     3121 ccagaaccct gtggagtacg aaggtacgtt ccctctccca gaagcccagc ttgacaggtt
     3181 cttgatgaag atcagcttgg gctaccctac agaaaacgaa gaaaacgaaa ttctcgtaag
     3241 acttcagtac acccatccgg tggaaaccat cgagtccgtt gcagatggaa gcagcttggt
     3301 acgcatgcag gaactggtga aaaagacgtt cgtagaggaa tcagtgcggg agtatatcgt
     3361 tcgtttagtt cacgcaacca gaaatcacag cgacgtctcc atcggcgcat cgcctagggg
     3421 ttcccttgcc ctgtacaaaa cagctcaagc ttatgccacc attaagggta gagagtacgt
     3481 catacccgac gatgttaagt accttgctcc gttaacattg agccatagaa taattatcaa
     3541 acccgaaagc agcctgagag gaaagacacc agagaaggtt ttagaagaga ttctggagac
     3601 cgtgcctgta gagctcgaaa gtctaaaatc ctaaactggt aatcttgaga gaaaggagta
     3661 gcaaccagag gtaatgccag tgtatcgcct ctgttgcacg tgcaaaaagt gccctactgg
     3721 agagccttaa tctggattgc gggtatttct gccttgcttt tcaaaggccc gttcatctat
     3781 ttcatgttct acctgctcct tttggtctgg ctgataccgc ccatgcttct gagaagcagc
     3841 gaaaaacatc tgcggattac cagagagatt gaaaccgacg cagtcttctt cggcgagcgt
     3901 atgaaggtca agatcaaagt gcaaaacgcc agtagatttc ccttggtctg gctgaagatc
     3961 agagaagcct gtcccagaca gatcggacct aaaatgagat ccagtgtgag ctgggtggtc
     4021 tctctgaagc caggcgaaac caaaacgctg gaataccaca tcttcggcgg taaaagaggg
     4081 gcatatgtaa tcgggccgat aaaactggag ttaggagata ttttcgggct ttacaaacga
     4141 gaacttgaac tgcagttgtt tgattttgtt acggtctatc caaaggtagt tcccatagaa
     4201 gagctggggc tcacttccac tcagcctata gggaatttga aacatcctca aaaaatctat
     4261 gaagatcctc ttaaaatcgc cgggctcaga gactacaagc ctggagattc gccgaagcac
     4321 atttcctgga aagctacggc aagacaggaa aagcttatgg tacgtgagta tgaagccacg
     4381 atgacactag atactatgat cctggttgac ttggacagca gagattatgt ttctgccacc
     4441 tatgaaaacg ctaaggagct ggcgatcagc gttgctgctt cgattacagc gcacctcaca
     4501 agaaaaaggc aggcaattgg tctggccacc aatggtgtgc caatggcgga agcatcggct
     4561 cattcggaca ccaggatcct tgatgatgaa ccgctggaga acatcatgcc caagaaaggt
     4621 cagggtaggc agttcttaga gattctctcc cgcatcgaag gtacaagcag cggagaatcg
     4681 tttgtcggtt tgtgccaaag gatgaaggca aagcttcctt ggggtaccac agttgttatg
     4741 atcgtcccaa gggatacaga cgagttgatg agatttgccc agtcgctgat tgaaagtggt
//
LOCUS       DAOSDA010000076         4767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11273_length_4767_cov_3.305815, whole genome shotgun sequence.
ACCESSION   DAOSDA010000076 DAOSDA010000000
VERSION     DAOSDA010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11273_length_4767_cov_3.305815"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PLW82_02685"
     CDS             complement(<1..760)
                     /locus_tag="PLW82_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ46212.1"
                     /translation="MRKKLLLLALVLLLSIASSAVKIEVWIVGWSNEHERTAENLIDR
                     EFTPITGIEVDILPLGWGDGDKVILAIISGDAPDIITSNVVELGIRGSLLDLKKTFGE
                     EYDWLEAQLFPAITKQVHFEGTRFGLPQNVSVMNACYRTDILAEMGMEIPETWDDVRA
                     MLPKLQANGRDAGFFYGSPSSNAIWGAYTLITQHGGNFFMPDGFTSAMDLPESIRGFV
                     EYIELYTKHNMPKAGAGITQFRTGEWVMFIEGYWT"
     gene            1307..1579
                     /locus_tag="PLW82_02690"
     CDS             1307..1579
                     /locus_tag="PLW82_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46213.1"
                     /translation="MRGKVRKIGLIVLLVCVLVGVAVLLNATSLGNKAASSALRAQGG
                     VMDTNKYNYIRDKMCLSWTVFGGIGFGLGGLGLLLLGYVYYKELES"
     gene            1659..2009
                     /locus_tag="PLW82_02695"
     CDS             1659..2009
                     /locus_tag="PLW82_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HOJ46214.1"
                     /translation="MANERIFNMAFAKVYPLLVQKAERKGRTKAEVDTVIFWLTGYDE
                     EGLQAQLEKNVTCETFFNEAPQMNPNAEKIKGVICGYRVEEIEDPLLQKIRWLDKLVD
                     ELAKGKPMEKILRS"
     gene            2215..2583
                     /locus_tag="PLW82_02700"
     CDS             2215..2583
                     /locus_tag="PLW82_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ46215.1"
                     /translation="MELRVIDQRFSICKLKELSQAVLTDEFSFAARTDEEISLVCSTN
                     SVPKDALKCDHGWKAFRIEGELDFALVGVLAKIAGLLAENGIPIFAVSTYNTDYILTK
                     EGVFPKAISILEQNGYTIKT"
     gene            complement(2613..3503)
                     /locus_tag="PLW82_02705"
     CDS             complement(2613..3503)
                     /locus_tag="PLW82_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOJ46216.1"
                     /translation="MLDHRLQTFLTLCETGNYTKTAEILNMTQPAVSQHIRYLENHYQ
                     VVLVSGKGKHFALTEEGKALREYAKTLSANSERIMFLLHRIKDQTKPMNFGATLTIGE
                     YMMPPIITRLLQEEPEISVSMYVENTDILLHMLWEGKIDFALLEGHFNHSQFEYKVIS
                     QERFVGVCSPQNPIASRNNDIRELLDQTLILREPGSGSRDILEQALYNQNLSTDDFRH
                     TIEVGNLNVIKDLCHHNMGISFLYWEAVKREISHGYLQTIPMPDFEITHPFSFVYLKN
                     SPDRSSIEYWFNKIMSLRLG"
     gene            3616..4623
                     /locus_tag="PLW82_02710"
     CDS             3616..4623
                     /locus_tag="PLW82_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HOJ46217.1"
                     /translation="MLKAKAPGILLSAGVAVVAMFLASLVPGDIIGATVMALLVGMAL
                     NPLLNKYKQLDEGVSYTARMILRIGIILMGVNLNFAEVISVGKYSLFLMLFTMATAFG
                     AGNLIGRLFGMNWKLTNLLSVSTAICGGSAVAAVGPVIKAKDEDIAYAISSTFIFDVL
                     TVVVIPWIGMALGMSTAGYGLWVGTVVNDTSSVVAAGYAFSEAAGHISVIVKLTRTLF
                     IIPYVLIFSFITERLEARSAGVSGRVPINWKKIFPYFIVLFLVVVALRSTGLIPTAIV
                     PALSTTSKFCMVMALAAIGLKTSFGEIRDIGFKPMILGFIVDTLVVLVSMGVQIATGK
                     F"
BASE COUNT         1252 a         1018 c         1183 g         1314 t
ORIGIN      
        1 aggtccagta tccctcaatg aacattaccc actcgccagt tctgaactgg gttattccgg
       61 cgcctgcttt tggcatattg tgttttgtgt agagttcgat gtactcgacg aagcccctaa
      121 tggattcggg caagtccatt gcggaagtaa agccgtctgg catgaagaag tttccgccgt
      181 gctgggttat aagcgtgtag gcaccccaaa tagcgttgga agagggactt ccgtagaaaa
      241 agcctgcgtc cctaccgttt gcctgcagtt tgggcagcat tgcgcggaca tcgtcccatg
      301 tttcggggat ttccatgccc atctccgcaa gaatgtctgt gcgatagcag gcattcataa
      361 cgctgacgtt ctgaggtaag ccgaacctgg tgccttcaaa gtgtacttgc ttggtgatag
      421 ccgggaaaag ttgtgcctcc agccaatcgt actcttcacc gaaggtcttc ttgagatcaa
      481 ggagcgaacc ccggattccg agctcaacaa cgttggatgt gataatatcg ggggcatcgc
      541 cggaaatgat ggccaaaatg actttgtcac catctcccca tcctagtggc aagatgtcga
      601 cttcgatacc agtaattggc gtaaactccc gatcgatgag attttccgca gttctctcgt
      661 gttcgttgct ccatcctaca atccagacct caattttaac agccgaactg gcgatagaaa
      721 gaagaaggac aagggccagg agaagtagtt ttttccgcat aacatcacct cgtttatata
      781 tatatctagt gccaatatta ggaaggtcgg caaaaactcc ttctcggatg aatgaaaaag
      841 attgaatatg ccaaacatac cgtagcaatt gccaactttt tggaaaatac gcatatctgc
      901 actactctgt gggattctcc ttgtcatttt tgttactaaa aactactaac actacttagc
      961 gtagctggac tgttgaacag tttatacgtg cgggaaaacc tttgatactt cggtttttcg
     1021 caattccgac tctaatcaac ctgacggttg gctgttccct ggtttttttg aaaaaagaga
     1081 aaggtttttt aacgcaagtg cataaaatct tttttgtcag ccgaaattcg ctgcttagct
     1141 gagggttttc ggtcaaacct ctgcaggata cctgttggca atggtaagtc agtgagggat
     1201 ctaacaagcg gttttaaacc gtatctcttt tggcgatagg ctaggcttag ccggtatcta
     1261 tatggcctga agcgtttgtt aagggcacaa gcgaggagga atcatgatgc gtgggaaagt
     1321 aagaaaaatc ggacttatag tgcttttggt ctgtgttcta gtaggagtgg ctgtgttact
     1381 caatgccaca agcttaggaa acaaagccgc aagttctgct ttgagagcac aaggcggggt
     1441 catggacaca aataaataca actacatacg tgacaaaatg tgtctcagtt ggaccgtttt
     1501 tggcggcatt ggttttggac tgggcggttt gggattacta ctcttgggat atgtgtatta
     1561 caaagaactg gagagctgaa gtaaaacttt tggtctagtt aaaacaacat ctgaagcaca
     1621 atataaacac gaaatcgtac gaagggaaat ggtacttcat ggcaaatgaa cgcatcttta
     1681 acatggcttt tgctaaagtc tatcctctgc ttgttcaaaa agcagagcgc aagggacgca
     1741 cgaaagcaga agtcgatact gtaatctttt ggctcaccgg atacgatgaa gaaggtctgc
     1801 aagcccagct tgaaaaaaac gtgacctgcg agacgttttt caacgaagct ccgcagatga
     1861 accccaatgc agaaaagatt aaaggggtga tttgcgggta ccgcgttgag gagatcgaag
     1921 accctctgct gcagaagatc aggtggctgg ataagctagt tgatgaactg gcgaaaggca
     1981 aacctatgga gaagatcctc cggagttaac gccagaaagt gcttaacaga agaaaaactc
     2041 tcctggcttt gccgggagag tttttactag ttgtggcgag cttggtcctg gtctcacagc
     2101 caggtggtag gagtagagaa gcctttccgc ttgtgagaga ccgaaagaaa gcggtgctga
     2161 aagtaaacgt cagtttagag aagagctaga aaccatttac tgaggtggtg tagggtggag
     2221 cttagagtca ttgaccagag attttccata tgcaagctta aagagttgtc gcaagcagtg
     2281 ttaaccgacg agttttcttt tgcagccagg actgatgaag agatttccct ggtctgcagt
     2341 acaaactcgg tgccaaaaga tgcgttaaaa tgcgatcacg gctggaaagc tttcagaatc
     2401 gaaggcgaac tggattttgc gctggtgggc gtattggcca agatcgccgg tctcctagct
     2461 gaaaacggca tcccgatttt tgcagtatca acatacaaca ccgattatat tttgaccaaa
     2521 gagggagtct tcccaaaagc gatcagtatc ctcgagcaaa acgggtacac catcaaaacc
     2581 tgatgcctct ctggagctct ggcaaagtac tttcagccta acctcagaga catgattttg
     2641 ttgaaccagt actcgatgct cgaccggtca ggagagtttt taagatatac aaaactgaac
     2701 ggatgggtga tctcaaaatc cggcattggt attgtctgga gatatccgtg tgatatctcc
     2761 ctttttactg cttcccagta caaaaaactg atgcccatat tgtggtggca gaggtccttg
     2821 ataacgttga ggttgcctac ttcgatggtg tgtctgaagt catctgtgct gaggttttga
     2881 ttgtagagcg cttgttcaag tatgtcacgg gatccgctgc caggctctct aagtataagg
     2941 gtctgatcca ggagctcccg tatgtcattg ttgcgagaag caatgggatt ttgaggtgaa
     3001 cacacaccta cgaacctctc ctgagaaatc actttgtact caaattggct gtggttgaag
     3061 tgtccctcta agagggcaaa gtcgattttc ccctcccaaa gcatgtgcag gaggatatcg
     3121 gtgttttcaa cgtacatgga aacactgatt tccggttctt cttgcagcag tcgggttata
     3181 ataggcggca tcatgtactc tccaatggtg agggttgcgc cgaagttcat aggttttgtc
     3241 tgatctttaa ttcggtgaag caaaaacatg atacgttcag aattcgcact tagcgttttg
     3301 gcatactccc gcagcgcttt gccttcttct gtgagggcaa agtgcttccc cttaccggag
     3361 accaacacca cctggtagtg gttttccagg tagcgaatgt gctgcgaaac tgcaggctga
     3421 gtcatgttga ggatctcggc tgtttttgtg tagtttcccg tttcgcagag agtaagaaac
     3481 gtctgcaatc tgtggtccag catgggagac ctccttataa aaacaagtta tacaaatata
     3541 atatatcata attagaattt atgatactca gatgatatat tcaacatagt gttcatttct
     3601 tctaggaggt gcagtatgtt gaaagccaaa gcacctggaa tactcctaag cgccggtgtt
     3661 gctgtagtgg caatgttttt ggcaagctta gttccaggtg atatcatcgg ggcaacggtc
     3721 atggcgcttt tggtcggcat ggcgctgaat cctctcttga acaagtacaa gcagctcgac
     3781 gaaggagtca gctacacagc aaggatgatt ctccgaatcg gtatcatact catgggtgtt
     3841 aacttaaact ttgccgaggt cattagtgtc gggaaatatt cgctcttttt gatgctcttc
     3901 accatggcta cagcatttgg tgcagggaac ctcattggaa ggttgttcgg gatgaactgg
     3961 aagctgacga accttctttc ggtaagtaca gccatctgcg gaggttctgc agttgcagcg
     4021 gtaggtccgg tgatcaaagc taaagacgaa gacatcgcct acgcgatttc ctcaacgttc
     4081 attttcgacg ttctcacagt ggttgtaatt ccctggattg gaatggcttt gggaatgtca
     4141 actgcagggt acggactttg ggtcggcacc gtggttaacg atacttcgtc agtagttgct
     4201 gcaggatacg cgttttctga agcagcaggg cacatttctg tgattgtcaa actcacaaga
     4261 accctgttta tcatccccta cgtcctcatc ttttcattta ttaccgaacg tctggaagcc
     4321 cgttctgcag gagtcagtgg aagggttccc atcaattgga aaaagatctt tccgtatttt
     4381 atcgtgttgt tcttagtagt tgtggctctc cgcagcacag gtctgattcc gacagctatc
     4441 gtaccggcac tatcgactac ctcaaagttc tgcatggtta tggctcttgc cgctattgga
     4501 ctcaaaacca gcttcggaga aatcagagac attgggttta aaccgatgat tctcggcttc
     4561 atcgttgaca ctctcgtagt gcttgtatcg atgggcgtgc agatcgctac aggcaaattc
     4621 taggaagata gaacgttaac tcgttttgtg aaggacaatc cctaaaacct caaattgtta
     4681 aaagataaaa ccgtttgttt tgcagctgac aaaacaaacg gttttttgat ccttaacgaa
     4741 ccgactctct tcttagtggt acttgaa
//
LOCUS       DAOSDA010000077         4757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11309_length_4757_cov_3.264568, whole genome shotgun sequence.
ACCESSION   DAOSDA010000077 DAOSDA010000000
VERSION     DAOSDA010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11309_length_4757_cov_3.264568"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PLW82_02715"
     CDS             complement(<1..1239)
                     /locus_tag="PLW82_02715"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOJ46218.1"
                     /translation="MTTTVETSSIPFDAASERRVLGIVMQYPDLMDRVVSNLKEAHFY
                     LPENRLIFKRINELYREGVKLSSTALSRKLIQNGDMDEPSQFLKGLMDSFISRSELEP
                     SILSIKEEYAKRRILEATDEIAKLITGKSDLDLGAVQAKAQQLIFEATSIEADASNEV
                     KDLLEVLERCYTNLVARSEGTKPSGLTVGYPSVDAITTGFKNKDLIILAGRPSMGKTA
                     LALNWSMNIAKRGKPVLIFSLEMDDESIGDRFVISELFSYRTEDGSPIVTSFEYQTKL
                     DEEKMKYAAEAFNDLYRLPIKIVDKRGLTVDEIKAKARQVKAEEPGLSLIIIDYLQLI
                     KPRDNSKTSWALQVGEIVRELRDLAGELDLPIILLSQLNRGVELRENKRPLMSDLRDS
                     GNIEEFADVVCFVYRDEYYYP"
     gene            complement(1223..1972)
                     /locus_tag="PLW82_02720"
     CDS             complement(1223..1972)
                     /locus_tag="PLW82_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOJ46219.1"
                     /translation="MIKYTGANYLNIPQTLIEKYQPVIGPVAAMVWINLLYLSKEQVS
                     QWEEPLLALTGLAKEELITQLEILEEADLIEVPEKAESGVKVLLKEPKADLPEAETAA
                     AELIETKEPAAPQDPALDENKKKLEALYDYYHERIGLMSAMDFPLLNEWMEVRGMSPE
                     LIAKAIEVTSENAQFPSMKYLDGVLKNWYNAGIRTVSDLEKNKLRQPKGDKPAKLSSE
                     AAAVPQKPIYKDVDLEKVRKWKELFKDDYNG"
     gene            complement(2003..3151)
                     /locus_tag="PLW82_02725"
     CDS             complement(2003..3151)
                     /locus_tag="PLW82_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46220.1"
                     /translation="MKKGLLLLLILFIIFPASSFAEPLGEIGFNTSAYMEEVEKLADK
                     WEKDQEFFLNSAAYRLFISEGVLIHGMGNSPNFRFGKTLTYYNYSDPTIFFLPPEHKG
                     GILSLFAFYHGDRKTDEVNITLGDKSYVLTPQRATDEYLFNAYLQKRPVWDPGIALSV
                     YVPQGTRYVKLKADSVEVLFARVGFLELSRKAAVLPEMDREPTATVPLSVSNAPAASS
                     TVPTETKDAVPTDLVTAPEQPGGLAVRDNWFRFTSNVEKFYLDYPQDWSIVTSQGQSI
                     LELTSPSGTQKVVVTKDALFSQTLKGFADQVANAIGMERINQISQEAQGRLRRTARVS
                     LGDRAFYLDVLFVKQGDYVFTITAISPEENKDDYGKEVETILSSFTLL"
     gene            3511..>4757
                     /locus_tag="PLW82_02730"
     CDS             3511..>4757
                     /locus_tag="PLW82_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46221.1"
                     /translation="MRRTALLLAIVLTLSALVAAVEITAWVTYGGIMGAILQELIETD
                     FTPKTGIKVNYQPYKIDTAFFQKALLALASGDAPDVMTLSAPQVIDLAIRGAIVDLTE
                     FPDFAEAVKDIYPGAMRSLTFKEHVFSVPFELGWTIAFYRTDVFSDLGISVPSTWDEF
                     KAIIPKIKAQGKEPYLSTISNDPEGHVRAFFPFIYQHGSDIFTEDGLRSNLDSPEALD
                     AFKNFTSFFTDYRLPVELPEIQAFSDGTSPYMVALNWIYAYLVKGQAQLLGKWTIAQA
                     PGTIRSDGTLDRTVPISSYAFAIPKNSNSEKVRAAWEFLKWIASDEVQREFQKRVHES
                     PEGWYLVFGTKGTANSEIFPENVRAVVDASLTESLGAMPVVGGYQTYRYISFAFSKVI
                     TQNADPEQALLEAARESNLELAR"
BASE COUNT         1093 a         1163 c         1198 g         1303 t
ORIGIN      
        1 gggatagtag tactcatctc tgtacacaaa gcagaccacg tcggcgaact cctcgatgtt
       61 gcctgagtcg cgaaggtcgc tcatgagcgg tctcttgttt tctctgagtt ctacgccgcg
      121 gttcagctgg gaaaggagaa tgatgggaag gtccagctct cctgcaaggt cacggagttc
      181 tctgacgatc tcgccgacct gaagtgccca gctggttttg ctgttatctc tgggcttgat
      241 cagctggagg tagtcgatga taatcaggga cagtccaggc tcttcggcct tcacctgcct
      301 ggctttggct ttgatttcgt caacggttag accgcgcttg tcgacaatct tgatcggcaa
      361 gcggtacagg tcgttaaaag cctctgcggc gtacttcatc ttctcctcat cgagctttgt
      421 ctggtactca aacgaggtta caatgggaga accgtcctcg gtgcggtagg agaaaagctc
      481 gctgataaca aatctgtcgc cgatagattc gtcatccatc tctagactga atattagcac
      541 aggtttgccc cgtttggcta tattcatgga ccaatttagg gctaaagccg tcttacccat
      601 agacggacgg cctgccagaa tgatcaggtc tttgttcttg aagcctgtgg ttatggcatc
      661 aactgaagga taccccactg taagccctga aggctttgtg ccttcagatc tggctaccag
      721 gttggtgtag cagcgttcga gtacctctag gaggtctttg acttcgttag aagcgtcagc
      781 ttcgatgctt gtagcttcaa agatcagctg ctgggctttt gcctgaactg caccgagatc
      841 caggtcgctt ttcccggtga tgagctttgc gatctcatcg gtagcttcta ggattcttct
      901 tttagcatac tcctctttta tagacagaat ggaaggctct agctccgatc tgctgataaa
      961 agagtccatg agccccttca gaaattggga cggttcatcc atgtccccgt tttggataag
     1021 tttcctggaa agggccgtgc tagacagctt gaccccttcc ctgtacagct cattgatcct
     1081 tttgaagatg agccggtttt cgggaaggta gaaatgagct tctttaaggt ttgagaccac
     1141 ccgatccatc agatcgggat actgcatcac aatgccgagc acccgtctct ccgacgcggc
     1201 atcaaacgga atcgagctgg tttcaaccgt tgtagtcatc tttaaacagc tccttccact
     1261 ttcttacctt ttctaaatcc acgtctttgt aaatgggctt ctgcggcacc gcagcagcct
     1321 cgctggaaag cttcgccggt ttgtcgcctt tcggctggcg cagcttgttc ttttccaaat
     1381 cgcttacagt tctaatgcct gcattgtacc agtttttgag aactccatct aggtacttca
     1441 ttgaggggaa ctgagcgttt tcgctggtca cttcaatggc tttagcgatg agttcggggc
     1501 tcatacccct aacttccatc cactcattga ggaggggaaa atccatggcg gacatcagtc
     1561 cgatcctctc gtggtagtag tcgtaaagcg cttcgagctt ctttttgttc tcatctaatg
     1621 ctggatcttg gggagccgca ggttcttttg tctctatgag ctctgcagct gcagtttctg
     1681 cttcaggcag atctgccttg ggctccttga gaagcacctt gaccccgctt tctgccttct
     1741 ctggcacctc tataaggtct gcttcctcta aaatctccag ttgggtgatg agttcttctt
     1801 tggcaagacc tgtaagggcg agtagtggct cttcccactg ggagacttgc tcttttgaaa
     1861 gatacaggag attgatccaa accattgctg caaccggtcc aataacaggt tggtactttt
     1921 ctattaaagt ttggggaatg ttgagatagt ttgcgccggt gtatttgatc attgggctgc
     1981 cctcctttgg acctgaagca tattaaagca gcgtgaagga agaaaggatg gtctctactt
     2041 ccttcccata atcgtcttta ttttcttcag ggctgattgc cgtaatggtg aagacgtaat
     2101 cgccctgctt gacaaacaaa acgtcgagat agaaagccct gtctcccagg cttactcttg
     2161 cagttcgcct taggcgtcct tgagcttctt gggagatctg gttgatcctc tccatgccga
     2221 tggcattggc cacctgatcg gcaaagccct tcaaggtctg gctgaagagc gcatccttgg
     2281 tcaccacgac cttttgagta cccgagggag aggtgagttc gagaatcgac tgcccttggc
     2341 tcgttacaat agaccaatcc tgtggatagt ccaggtagaa cttctccacg ttggaggtaa
     2401 atctgaacca gttgtcccgg actgcaagtc ctccgggctg ctctggagct gtaaccaggt
     2461 cggttgggac tgcgtctttg gtttctgttg gaacggttga gctggctgcc ggtgcgtttg
     2521 ataccgaaag cgggactgta gctgtaggct ctctgtccat ttcagggagc actgcagctt
     2581 ttcttgaaag ctccagaaag cccactctgg caaacaaaac ttccacagag tctgccttaa
     2641 gttttacata gcgggttccc tggggaacgt acacgcttaa agcaataccc ggatcccaca
     2701 ctggtctttt ctgcaggtag gcgttaaaca gatactcatc ggttgctctt tggggggtga
     2761 gaacgtagct tttatctccg agtgtgatgt ttacctcatc agtttttcga tctccgtggt
     2821 aaaaagcgaa caaggatagt attcctccct tgtgttctgg cgggaggaag aagatggtgg
     2881 gatcggagta gttgtagtag gttaaggttt tgccgaagcg gaagttcggc gagtttccca
     2941 taccgtgaat gagaacgcct tcagaaataa acaagcggta tgcggcggag tttaaaaaga
     3001 actcctgatc cttttcccat ttgtcagcaa gcttctctac ttcttccatg taggcggacg
     3061 tgttgaaacc aatctcgcct aatggctcgg caaagctact tgcaggaaag ataataaaaa
     3121 ggatgagtaa aagcagaaga ccttttttca tgtaaccccc tcctctgttt acttctgcaa
     3181 tgagggctgt ttaccttgta gcgaaacttt tggatcgtaa tgacacggca agttctaaat
     3241 gcgatagtta cggtcttttg taatttaatt gtactcttac ctttaaaaaa attaaactcg
     3301 ccctcccccc tcttgaagga gacctaatgg caaaagccac ctagatgtac gtcagagtac
     3361 atcaatctgg ttacaagaca ctgccagaag tccaactagg caaatcagcg gttgttaagt
     3421 tcctgacacg gtttctccgg attctgcagt ttgcagggaa aatttacagc agcgtcccaa
     3481 aatatacaaa actactaggg ggggttaatc ttgcgcagaa cagctctttt gttagctatt
     3541 gtactaactt taagcgcatt agttgctgca gttgagatta cagcatgggt tacttacgga
     3601 gggatcatgg gggcgatcct tcaagaactg atcgaaacag actttacacc gaaaacaggc
     3661 attaaggtga attaccagcc gtacaagatt gacacggctt tcttccagaa agcactgctg
     3721 gcgctggcat caggagacgc accagatgtc atgaccttga gtgctcctca agttattgac
     3781 ctggccattc gcggtgctat tgtggactta accgagttcc cagattttgc tgaagcggtt
     3841 aaggatatct atccgggagc tatgaggtcg ttgaccttca aagaacatgt gtttagtgtg
     3901 ccgtttgagt tggggtggac cattgccttc taccgcactg acgtcttcag cgatctgggt
     3961 atcagtgtgc cgagcacctg ggacgagttc aaggccatta ttcccaagat caaggctcaa
     4021 ggcaaggagc cgtatctttc caccatcagc aacgatcctg aaggccatgt gcgagcgttt
     4081 ttcccgttca tctatcaaca tggctcggac atcttcaccg aagacgggct ccgttccaat
     4141 ctagattctc ctgaagcgtt agatgcgttc aagaacttca cttcgttctt caccgattac
     4201 cggctgccgg tggaactgcc ggagatccaa gctttctcag acggcacttc accgtacatg
     4261 gtggcgctga actggattta cgcttaccta gtgaaaggcc aggcccaact gctgggcaag
     4321 tggaccatcg cccaggcacc aggcaccatc agaagcgacg gcaccttaga tcgcacagta
     4381 cctatctctt cctatgcatt tgctattccg aaaaatagta acagcgaaaa ggttcgcgct
     4441 gcgtgggagt ttctaaagtg gatagcttcc gacgaagtgc agagggagtt ccagaagaga
     4501 gtccacgagt ctccagaggg atggtacttg gtctttggaa ctaagggtac cgcaaactct
     4561 gagatcttcc cggagaatgt acgggctgtg gttgatgcgt cgctcactga gtctttagga
     4621 gccatgccgg ttgtgggcgg ctatcagact taccgctaca tcagctttgc cttctccaaa
     4681 gtgatcaccc agaacgcaga tccagagcaa gctctgctcg aggctgccag ggagtccaac
     4741 ttggaacttg caaggaa
//
LOCUS       DAOSDA010000078         4732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11389_length_4732_cov_3.367543, whole genome shotgun sequence.
ACCESSION   DAOSDA010000078 DAOSDA010000000
VERSION     DAOSDA010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11389_length_4732_cov_3.367543"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLW82_02735"
     CDS             complement(<1..455)
                     /locus_tag="PLW82_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46222.1"
                     /translation="MKRKVGVLVVLLLLCFTLAANAVAKELMIWLVASTEQAKLIKEL
                     TDNHWTPQTGVEVLFSTMTWDDALSRIYLAMASGDVPDIAQMGSTWQCEFGLRGGIFD
                     LAQAYPEETQEIMDRMYPGALTPIKFRGTTWGVPASLGNMTTYVRDDIFA"
     gene            756..1475
                     /locus_tag="PLW82_02740"
     CDS             756..1475
                     /locus_tag="PLW82_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46223.1"
                     /translation="MKRILLLMVVFLSLQCSAALIITPAIVEGVAGDFPETLTVDIYN
                     QGQEAVVLEVEVFQLNQKPSGVIAQGPSGNTRLTPERTTVVVEGAGKGEISFARDNAE
                     DSLYEVAIIKQTSLTQSTIAQAIAVPFILKKGSSEVFASLDSVNYDRETGIWEVIVNN
                     KGPEHFKAGITLAVQGESGPNTGSVQGIVLPGVARVFTLELPLDSSKGEGQLEVELGN
                     SSQTYCIRIDSNGAVECIEQK"
     gene            1472..4429
                     /locus_tag="PLW82_02745"
     CDS             1472..4429
                     /locus_tag="PLW82_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46224.1"
                     /translation="MSFVVAVVVVLSLLGCASSGELLDINLSGAPNSCGSQVVESINL
                     FVDSEGDWELAVSAEELRAQAEGREVVLPPDRLSVRVNDGPWVKLQKTGQLLLSGSGP
                     SGVKLEYRFDPLWEDSCHGEVYDTRLTYDLSSGRLFASFCSPGSAAPGDSVKIVFARE
                     KLEKAEVQVEKDGEVVFQQAVTNAKQVQIPTKHPNGTFWGSGRYDYQVSSFGAMITKG
                     SFSVCDLPERGAIRSATVKSEEEILFVEGSIVPTAVQEGSRAVLSLDICNLSAEPLED
                     ILVEITLPEGAVWFANSERRWTKAYKELAGLEKTHISLDFAVFPSAKKTEYACEVKVS
                     VTQDDGRHIVAAKSLAFQVKRGLLSEPGIVVGEVPDAVYFADGTKVVPQDGYFSLQLE
                     PGSYGVLLGDRPYVLNVSRYSERTTLAVGDRKKLGLIELKADISKSPAFGVKGFCQTD
                     HLQLAFVYPYKNFQNYHHTSYPSWGTKQRILERTPWQMNFSRGDFHFTAGTVYSSSPL
                     EGPFSSAALVGAEVGLAPGNGLQLQAVGGFHLEKLVEEEFLADGTTGPYLLKSAPTQG
                     TVKVIVESRNSRNQVVKTSTWDFYVDGKSVRLKKPLAESDQLGLKNYLVVSYVSETEK
                     RSDKFFGGGHVGFRQGESGLFLYRHLQDEGIMGTLSYKGLTIGGNLGSNAQEVSLAVT
                     GSTARHQLQLASTDGIGLAYKFDYHHNFDAGILISGKTGEAISKGELAFDFENSLFKA
                     TLRQKLIKEKEQTRDFSGEYAFYSGSLQWPKNLTWSISLEKFNGRKSESFSTRMMYKL
                     LGFSGYVEGTWRFADGRPSVFVNLSHDKVPLTVNAEWEHISPEFSLLEVCYDTKAFWT
                     KLSLCAEKAGLKRTLGVTVVPKEGLLYLGLSGDDLKTEYHLPLSRTGFKLSVSGNLDT
                     KILVMASELDVALSQRSHIGVSGAWESGLNNSFRLEGFYRFKTDNLQLETRLSERETG
                     VYLTYLIPLGRSS"
     gene            4496..>4732
                     /locus_tag="PLW82_02750"
     CDS             4496..>4732
                     /locus_tag="PLW82_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46225.1"
                     /translation="MKKWLLLAVLLCVAVGVRADFFTQQVTAKIEPMIQIEWPDAEFS
                     FGDLVAGPNETPVMPLKVISNIPYELELSTESICL"
BASE COUNT         1231 a         1042 c         1261 g         1198 t
ORIGIN      
        1 gcaaagatgt cgtctcgcac gtaagtagtc atgttgccta aggatgccgg aactccccaa
       61 gtggttcccc tgaacttgat cggagtcagt gctccagggt acatacgatc catgatttct
      121 tgagtttcct cagggtacgc ttgggctaag tcgaatattc cgccgcggag tccgaactcg
      181 cactgccagg tggatcccat ctgggcgata tccggtacat ctcccgaggc catcgctaga
      241 tagattctgg aaagtgcgtc gtcccaggtc atggttgaaa agagcacttc aacaccagtc
      301 tgcggtgtcc agtggttgtc ggtaagttct ttgatgagtt tcgcttgttc agttgatgcc
      361 acaagccaaa tcatcaactc tttggccacg gcatttgctg ctagcgtgaa gcagagaagt
      421 agcaaaacga ccaaaacccc tactttgcgt ttcacagtga atccccctca caaatggttt
      481 ttcagagggg tcttcccctc tgacttctga ttggttaaca gatgaatgat ttgtaaaaat
      541 ggtccataaa aagatttctt gccttatgta aaacttcctt ttttctctga cgaattacaa
      601 acgaatctaa aaaaagaagg acatatctag aaaacgtaga aatagctaaa tacatattag
      661 tcccggttaa gccaggacaa caaagatgcg catcgagaaa gggctcttcg ataccgaggg
      721 gccctttctt cttccgggag aaaggagtta ctttcgtgaa gcggattttg cttttgatgg
      781 tagtgtttct ttctctccag tgtagtgcag cattgatcat tacaccggca attgttgaag
      841 gtgttgctgg tgacttccca gagaccttga cagtggatat ctacaaccag gggcaagaag
      901 cagttgtttt ggaagtagag gtgtttcagc taaatcaaaa accgagcgga gtgattgctc
      961 agggcccgtc cggcaatact cggcttactc cagaaaggac cacagtggtg gtagaaggag
     1021 caggcaaagg tgagattagc ttcgcccgag acaacgctga agactctctg tatgaggttg
     1081 cgataatcaa acagactagt ctcacacaaa gcacgatcgc tcaagcgatt gcggtgcctt
     1141 ttatcttgaa aaagggaagc agtgaggttt tcgcaagcct ggatagcgtt aattatgatc
     1201 gggagaccgg gatttgggaa gttatcgtca ataacaaggg tccggagcat ttcaaagccg
     1261 gaattactct tgctgtgcaa ggagagtcgg gaccaaatac cggctctgtc caggggatcg
     1321 ttcttcctgg agttgccaga gtatttacat tggaacttcc gcttgattcc tctaaaggtg
     1381 aggggcagtt agaggtagag ctaggaaaca gcagccagac ctactgcatt cgcatcgatt
     1441 ccaacggcgc tgtagaatgc attgagcaaa aatgagtttt gttgttgcag ttgtcgtagt
     1501 tctttctctt ttgggttgtg cttcgagcgg agagctgctc gacataaacc tatcaggtgc
     1561 ccctaacagc tgcggcagcc aggttgtcga atctataaat ctttttgtag atagcgaggg
     1621 agactgggaa cttgcagtat cggcagaaga actccgagca caagccgagg gcagggaggt
     1681 agtgcttcct ccagataggc tcagtgtgag ggtaaacgat gggccatggg ttaagcttca
     1741 gaagaccggc caacttcttc tatctggcag cgggccttct ggagtcaagc tcgaataccg
     1801 cttcgatccc ctatgggaag actcatgtca cggagaggta tacgacacac gcctaacata
     1861 cgatctttct tccggtaggc tttttgcatc tttctgttca ccaggaagcg cagctcccgg
     1921 agatagtgta aagatcgttt ttgcccgtga aaagctggaa aaggcagagg tccaggtaga
     1981 gaaagatggc gaggttgtat ttcagcaagc tgtaactaat gccaaacagg tgcagatccc
     2041 cacaaaacat cccaacggca cgttttgggg aagcggcagg tacgactatc aagtatcatc
     2101 ttttggcgcg atgataacca agggaagctt cagtgtttgt gatctacctg agcggggtgc
     2161 gatccgcagt gcaaccgtaa aaagcgaaga agagattcta tttgttgaag gctctatagt
     2221 tcccaccgca gttcaagaag gaagtcgagc ggtcctgagc cttgacatct gcaatctctc
     2281 agctgaacct ttggaggata ttttggtaga gatcacgctg cctgaaggtg cagtttggtt
     2341 tgcaaatagc gagcgtcgct ggacaaaggc atacaaggaa cttgctggct tggagaagac
     2401 ccatatttct ttggactttg ccgtttttcc ctctgccaaa aaaaccgaat acgcttgtga
     2461 ggttaaagtc agcgttactc aagacgatgg caggcatatc gtcgcggcga agtccttggc
     2521 tttccaagta aagcgaggtc tactcagcga gccgggaata gtggtaggag aggttcccga
     2581 cgcggtttat ttcgcggatg gtactaaagt tgtgccgcaa gacggatact tttccctgca
     2641 gctagaaccg ggaagttacg gcgtcttgtt gggagaccgg ccgtacgtcc taaacgtttc
     2701 gcgctattcc gaaaggacaa cccttgcagt gggagatagg aaaaaattgg ggttgataga
     2761 gctgaaagcg gacatctcta aaagccctgc ttttggggta aaaggctttt gccagacaga
     2821 ccacctgcag cttgcgtttg tctatccgta caagaacttc caaaattatc atcatacctc
     2881 ttacccctca tggggaacga aacagaggat acttgaaaga acgccctggc agatgaactt
     2941 ctctcgtggc gactttcact tcaccgcagg cacagtctac tcttcttctc ctttagaagg
     3001 ccctttttca tcagctgcac tagtgggagc agaagtgggt ctggctccgg gaaatggctt
     3061 gcagctacaa gccgttggtg gctttcatct ggaaaagctg gtggaggagg agtttttggc
     3121 agacgggacc acaggtcctt acttgttgaa gtctgcaccg actcaaggta cggtaaaggt
     3181 gattgtagag tctagaaaca gccgtaacca ggttgtgaag acttccactt gggacttcta
     3241 tgtagatggg aagtccgtaa gactaaaaaa gcccctagct gaaagcgatc agttgggtct
     3301 gaaaaactac cttgttgtgt cctacgtttc cgagacagaa aaacgcagcg ataagttttt
     3361 tggcggagga cacgtcggat ttagacaggg tgaaagcggc ctttttctct acaggcacct
     3421 gcaggatgaa gggatcatgg gcactcttag ctacaaaggc ctgactattg gtgggaatct
     3481 gggaagcaac gcacaggaag tttctttagc ggtgacaggt tctactgcgc gccatcaact
     3541 gcagctggct agtaccgatg gaattggact tgcttataag ttcgattacc atcataattt
     3601 tgatgcaggc attttgatat ccggtaaaac tggcgaagct ataagtaaag gtgagttggc
     3661 gttcgatttt gaaaacagcc tgtttaaagc cacgctgagg caaaagctga ttaaagaaaa
     3721 ggaacagact agagacttct ctggggagta cgccttttac tcaggctccc tgcagtggcc
     3781 aaaaaacctt acttggagca ttagcttgga aaagtttaac ggtagaaaaa gcgagagctt
     3841 ttccaccaga atgatgtata agcttctggg tttttccgga tatgtcgaag gaacttggag
     3901 gtttgccgat ggtagaccta gcgtttttgt aaacctctct cacgacaaag tgccacttac
     3961 tgtaaacgca gagtgggagc acatcagccc ggagttctcc ttgctggaag tctgttatga
     4021 cacgaaagcg ttctggacca aactttctct ctgcgccgag aaggccgggt tgaagagaac
     4081 ccttggagta accgttgtgc ccaaagaggg gcttctgtat ctggggctct ctggagacga
     4141 cttgaagacc gagtaccacc taccgctttc gaggaccggc ttcaagttat ctgtatcggg
     4201 gaatctggac accaagatcc tggtaatggc atcagagcta gacgtcgcgc tttctcagcg
     4261 gagccacatt ggcgtttccg gggcttggga gtctggacta aataacagtt tccggttgga
     4321 gggtttctat agattcaaga ctgacaactt acagctagag acacgcctta gtgaaagaga
     4381 gaccggggtt tatctcacct atctcattcc tcttggacgg agtagttagg gagattccca
     4441 gcagaaatca ctaaccggca ctaatcaatc tctagaaagg atgagttgtt aggcaatgaa
     4501 aaaatggttg ttgttagcgg ttttactgtg cgttgcggta ggagtcagag ctgacttttt
     4561 tacgcagcag gttacagcca agatcgaacc gatgattcag atcgagtggc ccgatgcgga
     4621 gttcagcttt ggagatcttg ttgcaggtcc aaacgagact ccggttatgc cactgaaggt
     4681 gatctctaac atcccatacg agttggagtt gtccacagag agcatctgtc tc
//
LOCUS       DAOSDA010000079         4730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11400_length_4730_cov_3.302674, whole genome shotgun sequence.
ACCESSION   DAOSDA010000079 DAOSDA010000000
VERSION     DAOSDA010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11400_length_4730_cov_3.302674"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..>271
                     /locus_tag="PLW82_02755"
     CDS             68..>271
                     /locus_tag="PLW82_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46226.1"
                     /translation="MLDMDVQIGRVESDFELQNRVTRPGQVVNQNSIPDGYLQGSGRF
                     DIPDDKLTIKVVSGNLFIKEAETN"
     assembly_gap    272..281
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            453..734
                     /locus_tag="PLW82_02760"
     CDS             453..734
                     /locus_tag="PLW82_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46227.1"
                     /translation="MNMVLIVTMVVLFLVVLGIFTTYVEYKKRKLYYPSSVAYRTFLA
                     RGVDSVSVGFALYLGLYTLGRGLWLLPGLITLFAGNIYLIFYLLGKEGK"
     gene            736..1641
                     /locus_tag="PLW82_02765"
     CDS             736..1641
                     /locus_tag="PLW82_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOJ46228.1"
                     /translation="MRRFLLVILILLQVVIIWEIYATVKDADFELDFARIFDGGGVVM
                     NLSNVKHEEPFNQSFQAAKQVRLEGFVGDVDIVYADTSDIIFEGKKFLSHTTLDMEAF
                     DSTHIEYKIVNGTAILRWEKPRMFGGTVGFTGVVTVPRSVERLDVEVELGKVVSKGGR
                     NSLSIRANLGDVRVDGHEGSVVINADLGAVRLKDVHPGDVLRIDADLGEIDYSGSLAT
                     NTDIRASLGSVTLRLPEDTWTNIDASVDLGSLNSDFPFTNYWQSGSKTRIDGVLGEHR
                     PHAPNGKLTIRANLGSVNIRKVAMW"
     gene            1641..2009
                     /locus_tag="PLW82_02770"
     CDS             1641..2009
                     /locus_tag="PLW82_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46229.1"
                     /translation="MMLPVVLTLIVAGFITVIVVISSYFSYKEKEMLVKHGVDPEGVR
                     KIAYKEEYLEPITKLTSLRSAVTLIAVGGALTIGLGLIGVGPWLLGGLIPLFLGIGRL
                     ILYYILPDDKQEGKDENKDA"
     gene            2317..2910
                     /locus_tag="PLW82_02775"
     CDS             2317..2910
                     /locus_tag="PLW82_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOJ46230.1"
                     /translation="MSLKSQSHSVKWTRNLESLIDLYKKQKPVLEEIKLELKEIDGEL
                     EHELAVLLSDIDEKITGISQTSPELRSSLKNSGSFAVCELEENTACLAVFLEAAKATS
                     DAEKLQRGVCVRQCLKRVDYCLDQLLLFLSEPFRDLTSREQEILVNLAEGLSNEEIAE
                     KLYISIKTVKNHVSSIYRKTGFRERAKLVLAARSILG"
     gene            3001..4326
                     /gene="rimO"
                     /locus_tag="PLW82_02780"
     CDS             3001..4326
                     /gene="rimO"
                     /locus_tag="PLW82_02780"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOJ46231.1"
                     /translation="MAVALIHLGCPKNLVDSEVLLGYLSDEFTITDSLASADVIVVNT
                     CGFIESAKEEAIDTIFQAAEFKQDRCRALIVTGCLAQRYGRVLLDEIPEIDGILGVEE
                     LPRIREVVHKALSGQRPLCIERTGYLVSKGPRIRSTPSFSAYIKVAEGCLNQCAYCAI
                     PLIRGKQRSRPLEQIVEEAKSLVASGVREITLIAQDTTGYGLDLSSGDRLDVLIGRLS
                     DELPEDAWLRTLYLYPTRLTPQIISAMSSSKQFCRYMDLPLQHISEKVLKSMGRPFQK
                     EKTDELIRLIREKMPDAALRTTFLVGFPGETDADFAELMEFVKEIRFDHVGVFAFSPE
                     EGTKAYEMKPVVPKEIAQERADLIMKVQQEISREKNAARIGQVLEVLCTGVSEESELV
                     LTGRTRFQAPEIDGVTYFGIPDAEVKPGDVVNVKITQALEYDLAGDVII"
     gene            4380..>4730
                     /gene="pgsA"
                     /locus_tag="PLW82_02785"
     CDS             4380..>4730
                     /gene="pgsA"
                     /locus_tag="PLW82_02785"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOJ46232.1"
                     /translation="MNVPNLLTFLRIVLVPLFMVLALKDMPYGDFLAALVFSLAAITD
                     FFDGHIARKNKLVTKLGALIDPVADKLLISGALLCLVQLGRVHALLAMVVLAREFAVM
                     GLRLIAASSGVTIKV"
BASE COUNT         1277 a         1008 c         1241 g         1194 t
ORIGIN      
        1 caagcgttcc gagttcagaa gtgcgtctgg cgacctcacg ttgattctac cgagtgatac
       61 gatctgcatg ctcgacatgg atgtgcaaat cggccgtgta gaaagcgatt tcgaactaca
      121 aaacagggtc acccgacctg gtcaggtagt aaaccaaaac agtatccccg atggttacct
      181 gcaaggtagc ggaagattcg acataccaga tgacaaactg acgatcaagg tagtctcagg
      241 caacctcttc attaaagagg cagaaacgaa cnnnnnnnnn ngaactagtg tgtgttcact
      301 ggttcttggc ataaattggt tgttgtgaaa gcgatgatct atagctggtt gtacgcgagg
      361 ctgtttttgg ttagttgtgt ttttaggtgg atcaagtgca cggttacact ggctgatcca
      421 ccgcattttg tgaaaaggtg aggggatagg cgttgaacat ggtgctcatt gttacaatgg
      481 tggtgctgtt tttggtagta ctagggatct ttaccaccta tgtagagtac aagaagcgta
      541 agctgtatta tccatcaagc gttgcttaca gaacgtttct agccagaggt gtggattcag
      601 taagcgtagg ttttgccctg tacctagggc tttacacgct gggcaggggg ttgtggctcc
      661 ttccggggct tataaccctg tttgcgggca acatctatct aatcttctac ctcctgggaa
      721 aggagggtaa atgaagtgag aaggttttta ttggtaatcc tgatacttct gcaggttgtc
      781 atcatttggg agatctacgc tacggtcaag gatgccgact tcgaactgga cttcgctcgc
      841 atctttgatg gtggtggggt agttatgaac ctttccaacg tgaaacatga ggaaccgttc
      901 aatcaaagct tccaagcagc gaaacaagtt cgcttggaag gttttgtcgg cgatgtggac
      961 attgtctatg ccgacacttc cgacatcatc tttgagggca agaagtttct ctctcacact
     1021 acgctggata tggaagcttt cgacagcacc cacattgagt ataaaattgt gaacggcact
     1081 gcgatcttaa ggtgggaaaa gccgcgcatg ttcggcggaa ccgtaggctt tacaggggtt
     1141 gttacagtac cgagatctgt tgagcgtctc gatgttgaag ttgagttggg taaggttgtc
     1201 tcaaaaggag gccgtaacag cctgtccatc agagccaatt tgggcgacgt aagagtagac
     1261 ggacatgagg gatcggtagt catcaatgca gatctcggcg cggtaaggct taaggacgtg
     1321 caccctggag atgtactgag aatcgacgcg gatttggggg agatcgacta ctcaggttct
     1381 ctagccacaa acacggacat tagagcctct cttggaagcg ttacgctgcg tttgcccgag
     1441 gatacctgga cgaacatcga cgcatcggtg gatctaggta gcttgaacag cgactttcca
     1501 ttcactaact actggcaaag cggttcaaag acaagaattg atggcgtgtt aggtgaacac
     1561 agacctcatg cgccaaatgg aaagctgact atcagagcga atctcggctc tgtgaacatt
     1621 cgaaaggtgg cgatgtggta atgatgcttc cagttgtact gacacttatt gttgcggggt
     1681 ttattacagt tatcgtcgtc atctccagct acttttccta caaagaaaaa gagatgttgg
     1741 tgaaacacgg tgtggatcca gaaggtgtga gaaagatcgc atataaggaa gagtacctag
     1801 aaccgatcac caagcttacc agccttcgtt ctgcagtaac gctgattgct gtaggtggtg
     1861 cccttaccat cggtttgggt ctcattggcg tgggaccgtg gcttctcgga gggctcattc
     1921 cgctgttttt gggcatcggc cggttgattt tgtactacat cctgccagat gacaaacaag
     1981 aaggtaaaga cgaaaacaaa gatgcataga aatctaagaa ggatgccgct ttgggcatcc
     2041 ttttttgtgt cctcttgaca aaaaccacca aaaaggtgca aggagaacac cacggttttt
     2101 cgagctgtgg attcaaccca caaaggccaa agatacagac agtcctacag caaagcgtcc
     2161 tccttcagta ctaaatttcg tgagcagtat gcaaactgtg gctttaaact tttgcaactc
     2221 atattgtata acaagcgcca aaccaaataa aaaccactcg tagaaacagc aagtaaccgc
     2281 tttctttata gacctcttga aggagggcgt agcagaatgt ctttaaaaag ccaaagtcac
     2341 agcgttaagt ggaccaggaa tctcgaatct ctgattgatc tatataaaaa acaaaaacca
     2401 gttttagaag aaataaaact ggagttgaaa gagatagatg gggagttgga gcacgaactc
     2461 gccgttcttt tgtctgacat tgatgaaaaa ataaccggca tttcccagac cagtcccgag
     2521 cttcgaagct ccctgaaaaa ctcaggaagc tttgcagtat gcgagctgga agaaaacact
     2581 gcctgccttg cggtgttttt ggaagcagca aaggccacat ctgatgcaga gaaactgcag
     2641 aggggagtct gcgtcaggca gtgcctaaaa cgcgtcgact actgcttgga tcagctgctt
     2701 ttgtttttga gtgaaccttt tcgcgatctt accagcagag aacaggagat cttggtaaat
     2761 cttgcagaag gcctaagcaa tgaagaaatt gcggaaaagc tctacatcag cattaagacc
     2821 gtaaagaacc acgtaagcag catctatcgt aaaaccggtt ttagagaaag agcgaaatta
     2881 gtccttgcgg caagaagcat cttggggtaa ccttgaagaa tggagcgtac tcagccacca
     2941 ggcccgctat gtggtataat ttagataatc gatgcacaag gagtgttttt cttgcacaaa
     3001 gtggctgtag ctttaattca tctgggatgc ccgaaaaatc tagtggactc agaggtcctt
     3061 ttaggatact taagcgatga gtttaccatc accgattctc tagcgtcagc cgatgtcata
     3121 gtagtgaata cctgcggctt tatcgaaagc gctaaagaag aagcaatcga cacgatcttt
     3181 caagcagccg agttcaaaca ggaccggtgc agagcattga ttgtcacggg gtgtctggct
     3241 cagcgctatg ggcgcgtact tcttgatgag atacctgaaa tagacggtat attaggtgta
     3301 gaggagcttc ctcgcattag agaagtagtc cataaagcgc tctcaggcca gaggcctctt
     3361 tgcatagaac ggacaggcta tctggtatca aaagggccga gaattaggtc gactccaagc
     3421 ttttccgcct acatcaaagt agccgaagga tgcctcaatc aatgcgccta ctgtgccatt
     3481 cccctcatcc ggggcaagca gcggagcagg ccgctggagc aaatcgttga ggaagcaaag
     3541 agccttgttg ccagtggcgt aagggagatc acccttattg cccaagacac taccggttat
     3601 gggctggatc taagtagcgg agacaggctg gatgttctta ttggaagact ttcggacgag
     3661 cttcctgaag acgcgtggtt gcggaccctt tacctctatc ccacgaggct cacaccgcag
     3721 atcatctccg ccatgagctc ttccaagcag ttctgcaggt acatggattt acccctgcag
     3781 cacatttccg aaaaggtctt gaagagcatg ggcagacctt ttcagaagga aaagaccgac
     3841 gagctgattc ggctaatcag agagaagatg cccgatgcag cacttaggac aacctttctg
     3901 gtgggctttc ctggggagac cgacgccgac tttgccgaac tgatggagtt tgtcaaagag
     3961 atcaggttcg accacgttgg agtatttgcg ttttcaccgg aagaaggcac caaagcctac
     4021 gaaatgaagc cggttgttcc caaagagatc gctcaggaaa gagcggatct gattatgaaa
     4081 gttcagcagg agatctccag ggaaaagaac gctgccagaa tcggtcaggt gttagaggtt
     4141 ctctgcacag gagtgagcga agaaagcgaa ctggtgctca caggtagaac caggtttcaa
     4201 gcgccggaaa tcgacggcgt aacctatttc ggcattccag atgcagaagt aaaaccaggt
     4261 gatgtagtta acgtcaagat aactcaagct ctagagtatg atttagctgg agatgtgatt
     4321 atctagtata atagtattgg atgacctgta ggaggtgcag cctagattag gctagtgatg
     4381 tgaatgtacc caacttatta acgttcctgc gtattgtatt ggttccgctg tttatggtct
     4441 tggccttaaa agatatgccg tatggcgatt ttctcgctgc tttagtgttt tccttagctg
     4501 cgataaccga cttcttcgac ggccatattg cccggaagaa caaattggtt accaagctgg
     4561 gagcgctgat cgatcctgta gctgacaagc ttttgatatc tggggcttta ttatgtttag
     4621 ttcaactagg cagagtccat gctcttttgg ctatggttgt tctcgccaga gaatttgcgg
     4681 ttatggggct tagactgatt gcagcatcca gcggtgtgac gatcaaagtc
//
LOCUS       DAOSDA010000080         4729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11405_length_4729_cov_3.398160, whole genome shotgun sequence.
ACCESSION   DAOSDA010000080 DAOSDA010000000
VERSION     DAOSDA010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11405_length_4729_cov_3.398160"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /locus_tag="PLW82_02790"
     CDS             complement(<1..1800)
                     /locus_tag="PLW82_02790"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF000907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HOJ46233.1"
                     /translation="MKVVVAEHAGFCFGVERALKKAETAIQEHQTNVYTLGPLIHNPQ
                     VIARLQENGAKIVDSVAEVEDGTVIIRSHGVDPKIIEEAKVKGLNVVDATCPYVKRLH
                     EQAKLLKEQGYAVVIVGEKDHPEVQGLLGTLNHEATVVLGPEDLKDQRFDKVGVVAQT
                     TQSHKTFRDCVAYLVDTAREIRVFPTICQATESRQNSAVKLAGEVDVMIVVGGKTSGN
                     TRRLYELCLEVLPRVYHIERPDELEADWFKPTDTVGITAGASTPDWVIEGVVERMSEM
                     EKQEMQDVMPAAEESSEENQAVMMEELEKSQLKKGEVLEVTVVSFDKDGVYVDVGQKE
                     EGFIPLRELSYRYIESPDSAVSIGQKINVVVDRVEDGRIILSKKDADRETAWEFLTEA
                     KAEGKVFSAQVVEKVKGGLLVDIGVRGFVPASHVSRQFVEDLDQFVGQTMDFKVLELE
                     EERKNVVLSHRLVEEEELEKKKQEVFDSLEAGQIVDGVVRRITDFGAFVDIGSGVEGL
                     LHVSEMAWSRVDHPSDVVSEGDKIKVKILALDKDKDRISLGLKQTNADPWSLVESKYA
                     VGEIVEGTVTRVVDFGCFVKLEDGVEGLVHISQL"
     gene            complement(1797..2417)
                     /locus_tag="PLW82_02795"
     CDS             complement(1797..2417)
                     /locus_tag="PLW82_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ46234.1"
                     /translation="MIYAFLKAIARVLFRIFGKLKVEYRMPIPEGGVILACNHISDLD
                     PPVLAAASTRPVYFLAKKELFQNRLVGAILRGFKAYPLDRSKGDLAAIKRALGLLKEE
                     KVLGIFPEGTRVKGAQLGKPHNGAAFLAVQAKVPIVPVAISGTGPGFRGLFLGKVRMS
                     IEFLEPITVEQIEELVKKGDLDKHQKLGMISNEIMDRIGQALEGKM"
     gene            complement(2429..3082)
                     /gene="cmk"
                     /locus_tag="PLW82_02800"
     CDS             complement(2429..3082)
                     /gene="cmk"
                     /locus_tag="PLW82_02800"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOJ46235.1"
                     /translation="MALPVITIDGPAGAGKSTVAKKVAQELGLTYLDTGAMYRAVTLL
                     ALRRGVDVNDDAKMKELVRDIYLDLSGGRIYLNGEDVSEEIRRPEVSESVSLVARLGS
                     VRERMTALQREIASRGGVVLDGRDVGSVVFPEADCKIFLTASVEERARRRYLELQNKG
                     IAISFDEVKQNIVERDKIDSERDIAPLTIPEGAHIVDSTGLTIDEVVQEIVRKCRTD"
     gene            complement(3087..4391)
                     /gene="aroA"
                     /locus_tag="PLW82_02805"
                     /pseudo
     CDS             complement(3087..4391)
                     /gene="aroA"
                     /locus_tag="PLW82_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
     gene            complement(4394..>4729)
                     /locus_tag="PLW82_02810"
     CDS             complement(4394..>4729)
                     /locus_tag="PLW82_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ46236.1"
                     /translation="FLDVFQTLCAADHLTSYLQEACSIREKLPFKGRGVVGEIFSLVI
                     KVEDKPGAIGEVTSILAGRHINIKEIEILKQREGETGPLRLGFATAEKALEAYSLLKE
                     LGYTLETRW"
BASE COUNT         1074 a         1362 c         1040 g         1253 t
ORIGIN      
        1 cagctgggag atgtgtacca ggccttcaac gccgtcttca agcttcacaa agcagccgaa
       61 gtcgactact ctggtgacgg taccttcaac gatctcacca actgcatact tagattcaac
      121 caaagaccag ggatcggcat tggtctgctt gagaccgagg gaaatgcgat ccttatcctt
      181 atcgagtgct aagatcttga ctttgatctt atccccttcg gaaacaacgt cgcttggatg
      241 atctacgcga ctccaggcca tttcgctgac gtggaggagc ccctcaacgc cgctgccgat
      301 atcgacgaaa gcaccgaaat cggtgatgcg tctgacaacg ccgtcaacaa tctgccctgc
      361 ctcgagacta tcgaagacct cttgcttctt cttctcgagt tcttcttctt ccaccaatct
      421 gtgcgaaagc acaacgtttt tgcgctcttc ttcaagttcc aagaccttga agtccatcgt
      481 ctgaccgacg aactggtcta ggtcttcgac gaactgacgg gacacgtgac tggcaggaac
      541 aaaacctcta acgccaatgt caacaagcag tccgcccttg accttttcca ccacttgtgc
      601 agaaaagacc ttgccttctg ccttggcttc tgtaaggaac tcccatgcgg tttctctatc
      661 ggcatccttc ttggaaagaa taatccttcc gtcttccact ctgtctacca ctacgttaat
      721 cttttgtccg attgacactg cactatctgg agactctata tagcgatagc ttagttctct
      781 aagtggaatg aagccttctt ctttctgacc tacgtcgaca tagactccgt ccttgtcgaa
      841 gcttacaaca gttacttcga gaacttcgcc ttttttcagc tggcttttct caagctcttc
      901 catcataact gcttggttct cttctgaact ttcctctgct gcaggcatta cgtcctgcat
      961 ttcttgtttt tccatctcgc tcattctctc gacaaccccc tcaataaccc agtcgggcgt
     1021 ggaagcacca gcggttatcc ctacagtgtc tgtaggtttg aaccagtctg cttcaagctc
     1081 gtctggcctt tctatatgat acactctcgg gagcacctct agacagagct catagagcct
     1141 tctagtattc ccgctcgtct tgccgcctac cacaatcatg acgtcgacct ctccagccaa
     1201 cttgacggcg gagttctgtc ttgattctgt agcttggcat atcgtaggaa aaacccggat
     1261 ctcccgagct gtatcgacta aataagctac gcagtctctg aaagtcttgt gagactgtgt
     1321 agtctgggcc accacgccaa ccttgtcaaa cctctgatct ttaaggtcct ccggacctag
     1381 tactaccgtc gcttcgtgat tcagcgttcc gagcagtccc tgaacctcag ggtgatcctt
     1441 ctctccaaca atgaccacag catagccctg ctccttcagg agctttgcct gctcgtgtag
     1501 tcttttgaca tacggacatg tggcatcaac aacattaagg cctttaactt tggcctcttc
     1561 aataatcttt ggatcgacgc cgtgagacct gataatgacg gtcccgtctt caacctctgc
     1621 cacagaatcc acaattttcg caccgttttc ctgcaaacgg gctataacct gcggattgtg
     1681 tatcagaggg ccgagtgtgt agacgttagt ttggtgttcc tgtattgctg tttctgcttt
     1741 cttcagcgct ctttccacgc cgaagcaaaa acctgcatgc tctgctacga caactttcac
     1801 atcttaccct ccaaggcctg gccaatccga tccatgatct cattacttat catacccaac
     1861 ttttggtgtt tatccaagtc gcctttttta actaactctt cgatttgttc aacggtaatc
     1921 ggctctagga actcaataga catccttact tttcccaaaa aaagacctcg gaaacccggt
     1981 ccggtccctg agattgccac aggaacaatc ggaacttttg cctgaactgc taggaaagct
     2041 gcaccattgt gaggtttccc cagctgtgca ccttttaccc ttgtgccttc tgggaagata
     2101 ccaagaacct tctcttcttt gaggaggcct agagctcttt taattgccgc cagatcacct
     2161 ttagaccggt ccagcgggta ggctttaaaa ccccggagta tagctccaac caagcggttt
     2221 tggaaaagct ctttttttgc aagaaaatag accggcctgg ttgatgcagc agctagaacc
     2281 ggtggatcta agtcgctaat gtggttgcat gccagaatga ctccaccttc tgggatcggc
     2341 atcctgtact caaccttcag cttgccgaaa atcctgaaca aaactcgagc aatagccttg
     2401 agaaaagcat aaatcataag cggtactcct aatctgttcg acattttcta acaatttcct
     2461 gcaccacttc gtcaattgtt agtcccgtcg aatctacaat atgcgcacct tccggtatgg
     2521 tcaaaggtgc tatgtctctc tcagaatcta ttttatcacg ttcgacgatg ttttgcttga
     2581 cttcgtcgaa actgatagca atccccttat tttgaagctc caaatagcgc ctcctggccc
     2641 gctcctccac gcttgccgta aggaagatct tgcagtcggc ttcgggaaag accacgctgc
     2701 ctacatctct gccgtcgaga actaccccgc ctctagaggc gatttccctc tggagagcgg
     2761 tcatcctttc ccgcacagac ccaaggcgcg ctacaagaga tacgctctcg gaaacctcag
     2821 gccttcggat ctcttctgaa acgtcctcac cgttgaggta gatcctgccg ccgctaagat
     2881 caaggtagat gtcccgtaca agctctttca tctttgcatc atcgttgaca tccaccccgc
     2941 gtctgagggc tagaagagtc actgctctgt acatcgctcc cgtgtcgaga taggtcagcc
     3001 ctagttcttg ggcgaccttc ttggcgacag tgctctttcc agctcctgca ggtccgtcta
     3061 tggtaataac tggtagcgcc acgctatcac tcctcgatct gtgcaccaag cctcttcagt
     3121 tcgctgtaaa actgcggttg tgagacctcg gcgcattctg catttagaac cgtgattcct
     3181 tcggggctgt tgagtccggc aacgcatgca gccatcgcca ggcggtggtc gaacttcgga
     3241 tcgtaggttc ctccttcaaa ctcgcctggg cggccgtaga tctccaaggt aaggttctta
     3301 ccctcacctt ttatggcgca ctctcttcca agaagccgca ccaactcggc gatacccgaa
     3361 aggcgatccg actctttgac cttcaactcc gaaacgccgc agatggtagt tgtgccttct
     3421 gcagctgcag caagaaccgc aagaagcggg atctagtcga tgagcagtgg aacttcgacg
     3481 ctttctacaa cggttccgca aaggttctcc gatgcaacct tgatcctgcc aatgggctca
     3541 acatcttcgg tctcatctac caccaaagac gcccccatcc gttcgaggac tttaagcagc
     3601 cccgttcgcg ttggattcag gtttacatcg tctaaaataa tttcgctgcc ctccccgaga
     3661 agacctgcac cgatgaaaaa ggctgccgaa gaaaaatctc ctccaacctt cattttgatg
     3721 cccgaaagct tccgtccggg ctcgatgatg atctctgagc cctggcgggt gatctcggca
     3781 cccatgtggg cgaggatcct ttcggtgtga tctcgggacg gaatgggctc gattaccctg
     3841 ctttgacctt cactatggag ggaggcaaga atcacagcgg atttgagctg ggcagaggcc
     3901 acaggcagag tgaagtcagt gccttttagg tctcccccga caacggcaat aggcgcaaga
     3961 ccaccgcgcc ttgtgtgaat cacagctccc atgtcccgca ggggctcggc aattcttgcc
     4021 ataggccggt tgttcaacga gtggtctccg tacaaaaccg caaagatcct gctcggtgca
     4081 agaagcccca ttaaaaggcg catcaaagtg ccggagtttc cgcagtaaat agggcgatcc
     4141 ggctctttca aacctgcaat tccaggtcct ttagcaaaca acgtgtcgtt ttccaagcga
     4201 tactccaaac ccaaacaggc agcagcagac agagcagacc tgggatcgag agcactgctg
     4261 atcttctcga aaacgcactc ctcttctgta agcagactga acagaatcag acgttgggat
     4321 atggacttat cgctttccgg tctgattgct ccctgcagag gtttttggct ttttttaact
     4381 tttaggtcca tatctaccac ctagtctcta atgtgtagcc cagctctttc agcaggctgt
     4441 aggcctcaag cgctttttct gcagtggcaa agcccagcct gagaggtccg gtttctcctt
     4501 ccctctgctt taaaatctca atctctttaa tgttgatgtg cctaccggca aggatcgatg
     4561 ttacctcacc gatcgcacct ggcttgtctt ctaccttgat caccaaggag aagatctcgc
     4621 caacaacccc tcgtccttta aacggcagct tctcacggat gctgcaggct tcttggagat
     4681 aggacgtaag gtgatctgca gcgcagagtg tctggaaaac gtcaagaaa
//
LOCUS       DAOSDA010000081         4697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11507_length_4697_cov_3.486859, whole genome shotgun sequence.
ACCESSION   DAOSDA010000081 DAOSDA010000000
VERSION     DAOSDA010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11507_length_4697_cov_3.486859"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLW82_02815"
     CDS             complement(<1..771)
                     /locus_tag="PLW82_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOJ46237.1"
                     /translation="MEFSEIINKHYQSAVELRRELHRFPELSREEKETTRRIRDFLVS
                     KGFSTKEVELNGAKVQVSQPLETGLVVRIKRGEGGPVVAFRADIDALPVHEETGLSFA
                     SENPGRMHACGHDGHTAVLALTLLSFVEDTSWEGEVRGIFQPAEELYGGAERMVAAGA
                     LDGADYVYALHIWPEVPEGIVASRPGTIMASNDRFSVVLKGKSGHGAAPHLAKDPIVC
                     ACSLVTSLQSIVSREIAPWDGAVLTIGSICAGTAYNIIP"
     gene            complement(797..1972)
                     /gene="menC"
                     /locus_tag="PLW82_02820"
     CDS             complement(797..1972)
                     /gene="menC"
                     /locus_tag="PLW82_02820"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HOJ46238.1"
                     /translation="MSYLDERIKIDGIELYQVAIPLLVPFEISGGRSYNRQSIIIKMT
                     SGDLVGYGESAPFDAPFYSSETISSVKAMLHEWLIPKVVGKEFTSIEELNSVLAEGIR
                     GNNFAKAGLENAYWDLVAKKNNLRLVDAIKIKLAQMGVDQKHLEYKEYIESGVSVGIP
                     GDRSLDTLKTWVADYVEEGYRRIKIKIKPGWDIEPARAAREVIGPDFLFWVDANSSYE
                     LENSEVFREMDQLGCTFYEQPLNHDDILDHAALAKNVKTPICLDESLKSFRVGRQVIE
                     LGGIDVWNIKVQRIGGLLEGLKLYKLAVENNIKLWGGTMPESGVGAMPILALASFNGF
                     VYPADVEASARWYGMGKDLIEIEMDKKGRIYLPEGLGIGTEPNWQIMEKYGRKIASY"
     gene            complement(1985..3211)
                     /locus_tag="PLW82_02825"
     CDS             complement(1985..3211)
                     /locus_tag="PLW82_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46239.1"
                     /translation="MVKPRYFLPQEFEVSEEFQKALNEDYNRLIEEPESHIKDRYNID
                     VSSFYGPFKIKNPFGKASGQLSTNKGQVMADIEGGLGFTVLKTVIAEDQDKKAEMDEW
                     KIHAPKMVVEKLVSKNGEEGYSVVWKGRGWEKSLDDYLDFFDQCLAVAGEYPIIPSVK
                     YHLPGPSEEYKLSEYTYTTQKLLEVWKKHKNEDMVMEKDFSPTFAGTDVAGQKDMIIS
                     WLQDVPRLIKQSVDEPIHLGVKMMNATLEDEFQRELVRKFLGKDSSWLADFAICFNRL
                     FDHNKEFEGKVGVAYGGYDLSDRNLWALEEVLDDKPSRQVSATGNICSGKMMLEYALR
                     GAVNGQMHTFFQIPQSEYAPIDASRTRKAVHELLFNPQKGLVAGLIYIREKLGKGTEP
                     FTFLEVTDLNPYVLVK"
     gene            complement(3205..4155)
                     /locus_tag="PLW82_02830"
     CDS             complement(3205..4155)
                     /locus_tag="PLW82_02830"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ46240.1"
                     /translation="MALVLDRCLYPAVPVTFRKDGTIDYDALKKYAEWMDTQPITGIA
                     VWAHTGRGLMLSEEDREKVYKVYRSALSSDKKIICGVGSRNFTSEEEFIADAKKMGEH
                     AKALGADGILTYAPSFFRGKDNQDELIVKYHEALNSLGLDMVLFYLYEAAGGISYSLD
                     VLRKLFALENAVAIKMATLDSVMTYQDVSNLIAKEFPNKALITGEDRMFGYTMMRGGR
                     GALVGIGSAFTKLQKALIDAYYDQDYKAFVELTVLVDKLAEVTFIKPMEGYIERMLYI
                     CVLQGLVPESAAWDPYGPGVSEEERAAIREVVQELEGLGW"
     gene            complement(4237..>4697)
                     /locus_tag="PLW82_02835"
     CDS             complement(4237..>4697)
                     /locus_tag="PLW82_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46241.1"
                     /translation="SSEIFELSGAKVQFSAAALANTRYLHSHWGGHGRYTTGSEFLGD
                     DLGPDALKLGGILNLRTGGFSSSLSLAYEWHGPGHLDEYWTTIGRDEAYKTFFLSEIV
                     EKKLRAELAVGKSLTDSLQLQADLGVVYTTPKGKPATANFSGGVGLAVLF"
BASE COUNT         1057 a         1304 c         1076 g         1260 t
ORIGIN      
        1 gggaatgata ttgtatgcag tgcctgcaca gatgctgccg atggtcaaaa ccgcaccatc
       61 ccaaggagct atctctcggg atacgatgct ctgaagagat gtgacgaggc tgcaggcaca
      121 gacgatagga tcctttgcca ggtgtggcgc tgcgccatga ccgctcttcc ccttcaggac
      181 tacgctgaag cggtcgtttg aggccataat agtcccaggt ctgcttgcca caataccctc
      241 aggaacctca ggccagatgt gcagggcgta aacgtaatcg gctccatcca aagctcctgc
      301 tgcaaccatc ctctctgcac cgccgtagag ttcttctgca ggttgaaaga ttcccctaac
      361 ctctccttcc caggatgtgt cttcaacgaa ggaaaggagc gtgagtgcta gaaccgccgt
      421 gtgaccatcg tgcccgcagg cgtgcattct gccggggttc tccgacgcaa agctaagccc
      481 cgtctcttca tgcacaggta aggcgtcgat atccgctctg aaggccacca ccggtccgcc
      541 ttctcccctc ttaattctga ccacaagacc ggtttcgagg ggctgagaga cttgaacttt
      601 ggcaccgttt agctccactt ctttagtaga gaagcctttg gacaccaaaa agtccctgat
      661 ccgcctggtg gtttcctttt cctctcgtga aagctcggga aacctgtgca gttcccttct
      721 gagctcaacg gcactttgat aatgcttgtt gattatctcg ctaaactcca tgttcacacc
      781 tgcttcaaga ggtagtttaa tagctggcga tctttctacc gtatttttcc ataatctgcc
      841 agttgggttc ggttccgatt cccaaacctt caggcaggta gattcttccc tttttgtcca
      901 tctcgatctc tataaggtcc ttgcccatac cgtaccatct ggcactagcc tcgacgtcgg
      961 ctgggtaaac aaaaccattg aacgaagcga gagcgaggat cggcatggct ccaactcccg
     1021 actcgggcat ggtgccgccc cacagtttga tgttgttctc tacggctagc ttgtagagct
     1081 tcaaaccctc taagaggccg ccgattctct gaactttgat gttccaaaca tcaatgccgc
     1141 caagttcaat aacctgcctt cctaccctaa aagactttag agactcatcg aggcagatcg
     1201 gggtcttgac gttctttgcc agagctgcat ggtccaggat gtcatcgtgg tttagcggct
     1261 gctcataaaa ggtgcagccc agctggtcca tttcccggaa aacctcggag ttttccagct
     1321 cataggaaga gtttgcatct acccagaaca agaagtccgg cccgataacc tcacgggctg
     1381 cccttgcagg ttcaatatcc cagccgggct taatcttgat tttgattctt ctatagcctt
     1441 cctccacata gtctgccacc caggtcttca gagtgtcgag actccggtcc ccggggatac
     1501 ccactgaaac gccggactcg atgtactctt tatactcaag atgcttctgg tctacgccca
     1561 tctgagccag tttgatcttg atggcatcta ccagacgcag gttgttcttc ttggccacaa
     1621 gatcccagta ggcattttcc aaaccggctt tagcgaagtt gttgcccctt ataccttctg
     1681 ccagaacgct gttcagctct tcgatggagg tgaactcctt gcctacaacc ttcggtatga
     1741 gccactcatg gagcattgcc ttaaccgacg agatggtctc gctggagtaa aacggtgcgt
     1801 caaaaggcgc cgattctccg tagcccacca ggtcgccgga ggtcattttg ataatgatgc
     1861 tctgcctgtt gtagcttctt ccaccggaga tttcaaaggg taccaggagc ggaatggcca
     1921 cctggtacag ctctataccg tctatcttga tcctttcgtc taaataactc atcgtttgcc
     1981 acacctactt taccagtaca tatggattga gatctgttac ttcgaggaag gtgaaaggct
     2041 cggtgccttt ccctagtttt tcgcggatgt atatcagtcc tgcaaccaga cccttttggg
     2101 gattgaaaag caactcgtgc actgcttttc tggttctgct tgcatctatc ggagcatact
     2161 cactttgagg tatctggaaa aacgtgtgca tctgtccgtt taccgcaccg cggagagcat
     2221 actcaagcat catcttcccg gaacagatgt tgcctgttgc agagacctgc cgagaaggtt
     2281 tgtcatccaa gacctcttcc agagcccaga ggttgcggtc gctgaggtcg tagccgccgt
     2341 atgctacacc gactttgccc tcaaactcct tgttgtggtc gaacagcctg ttgaagcaga
     2401 tggcaaaatc tgcaagccag gagctgtcct tgcccaggaa ttttctgacc agctcccgct
     2461 gaaactcatc ttccaaagtt gcgttcatca tcttcacgcc caggtgaatg ggctcgtcca
     2521 cagactgctt gatgagcctt ggcacatcct gcaaccagga gatgatcata tccttctgtc
     2581 ccgccacatc ggtgccggca aacgttggag agaagtcctt ctccatgacc atgtcctcgt
     2641 ttttgtgctt tttccacact tccaaaagct tttgagtagt ataggtgtac tcactcagct
     2701 tgtactcttc agacgggcct ggcaggtggt actttacgct ggggataatt ggatattctc
     2761 ctgccacagc aagacactgg tcgaagaagt ccaggtagtc gtcgaggctc ttctcccagc
     2821 ctctgccctt ccacacaacc gagtagccct cttctccgtt tttggagacg agcttttcca
     2881 ccaccatctt gggtgcatgg atcttccatt catccatctc ggccttttta tcctggtctt
     2941 cagcgataac ggttttcaga accgtaaagc ccagaccgcc ttcgatatcg gccataacct
     3001 gccccttgtt tgtagaaagc tgacctgaag ccttgccaaa agggtttttg atcttgaacg
     3061 gcccgtaaaa gctggacaca tcgatgttgt acctgtcttt gatatggctt tcgggctctt
     3121 caatcagtct gttgtagtcc tcatttagag ctttttgaaa ctcttccgag acctcaaact
     3181 cctgaggcag gaagtaccga ggctttacca ccccaatccc tccaattcct gtacaacctc
     3241 tctgatggct gctctttctt cttcactaac ccccggtccg taaggatccc aagcggcgct
     3301 ttcaggcaca agaccctgaa gcacgcaaat gtacaacatc ctctcgatgt agccttccat
     3361 aggtttgatg aaggttacct ctgccagttt atccacgaga actgtaagtt ctacgaaagc
     3421 cttgtaatcc tgatcgtagt aggcgtcaat caaagctttc tggagcttgg tgaatgcaga
     3481 accgatacct acaagagcac cccttccccc acgcatcatg gtgtagccaa acatgcggtc
     3541 ttcgccggtg atcagggctt tgttggggaa ctcctttgcg attaagttgg atacgtcttg
     3601 gtaggtcatg accgaatcta aagtcgccat cttgatagct actgcgtttt ccagagcaaa
     3661 cagtttacgc agaacatcta ggctgtagga gattcctccg gcagcttcgt agaggtaaaa
     3721 caataccatg tcaagaccta gtgaattcaa agcttcatga tacttaacga tgagttcgtc
     3781 ttgattatcc ttccccctaa aaaagctcgg cgcatatgtg agaatgccat ctgctccgag
     3841 agctttagca tgctctccca tcttttttgc atcggcgata aactcttctt cactggtaaa
     3901 gttgcgggaa cctacgccgc agatgatctt tttgtcggag ctaagagcac ttctgtagac
     3961 tttgtagacc ttttcgcgat cctcttccga gagcatcagc cctcttcctg tgtgcgccca
     4021 aacggcaata cctgtaatgg gctgtgtgtc catccactcg gcgtacttct tcagcgcatc
     4081 gtagtcgatg gttccgtctt ttctgaatgt tacaggtacc gctggataga gacatctgtc
     4141 aagtactaaa gccattttac acataacacc tctttacact gatattgcta aacaaaaaag
     4201 cgttttctct tttttgtgat ccactatcct catctgttaa aacaaaacag caagcccaac
     4261 tcctccggaa aagttagcgg ttgcaggttt tcccttggga gtcgtgtaaa caacgcctaa
     4321 atctgcctga agctgcagac tgtccgtgag gctttttcca actgcaagct cggctcgcag
     4381 cttcttctcc acgatttcgc tgaggaaaaa ggttttgtag gcctcatccc ggccaatggt
     4441 agtccagtac tcatcgaggt gaccgggacc gtgccactcg taggctaggc tgagactcga
     4501 tgaaaaaccg ccggttctta ggttcaggat tccaccgagt tttagagcat ctggacctaa
     4561 atcatcaccg agaaactcgc ttcccgttgt gtagcgccca tgtcctcccc agtgggaatg
     4621 caggtaccgg gtattagcca gcgctgctgc agaaaactgg accttcgctc cggaaagttc
     4681 gaagatctcg gaactgt
//
LOCUS       DAOSDA010000082         4690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11532_length_4690_cov_3.030636, whole genome shotgun sequence.
ACCESSION   DAOSDA010000082 DAOSDA010000000
VERSION     DAOSDA010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11532_length_4690_cov_3.030636"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLW82_02840"
     CDS             complement(<1..323)
                     /locus_tag="PLW82_02840"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOJ46242.1"
                     /translation="MQKRTLSILIIAVLTLILLDGLFGFSKKLYSRYTATFGSTIHNE
                     NVAAEPPPEEDQPEPIRITLAAAGDIMVHLPQIKAAYNAETGTYEFTECFADIKELVQ
                     SVDLA"
     gene            complement(556..813)
                     /gene="spoVG"
                     /locus_tag="PLW82_02845"
     CDS             complement(556..813)
                     /gene="spoVG"
                     /locus_tag="PLW82_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOJ46243.1"
                     /translation="MHITDVQVRRVDEAGRMKAIASVVFNDVFVVRDIRVIEGDDGRF
                     LAMPSRKLPNGEYRDVAHPITADMREQLTKLVLEKLAELDA"
     gene            complement(1008..1772)
                     /locus_tag="PLW82_02850"
     CDS             complement(1008..1772)
                     /locus_tag="PLW82_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOJ46244.1"
                     /translation="MTKFDVVILAGAPNRGSLTEVSSATWEALVTVASRPMVEYVIDT
                     MLTLENVGKVVLVGPEELRETSPSISFTLCKDSLVDNLKAGIDALGADKSPYTLIVTS
                     DIPLISTEAVNDFIDQCLKHRDAKAFYPLVSREVNEASYPEVQRTYFPLKEGTFTGGN
                     IMLLDPNVVETHFELIRQLTDLRKSPAKIVKLLGLRFLVKFITKSLSIEEIEQRAEEI
                     LGFKAKAIISTYPEIGIDVDKPSDLYLMENLFAETH"
     gene            complement(1853..2533)
                     /locus_tag="PLW82_02855"
     CDS             complement(1853..2533)
                     /locus_tag="PLW82_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ46245.1"
                     /translation="MGKLGPIQLDNYQPLRELVFEAIREAIINGTLRPGERLMEEALA
                     EELGVSRTPVREAIRKLELEKLVIMVPRKGAYVADLTKSDVKEVFEIRRALEGLAAQL
                     AALRATDEQLEKLERYLVRIGEAIDREDIEQTIEIDEMFHDVLYKAAGNDRLEEMINN
                     LREHIQRYRSTSLAHPGRMKQAFVEHRKRVEAIGKRDSKLAKKLAEEHIANAESSLME
                     AIFQRGEK"
     gene            complement(2575..3558)
                     /gene="ispE"
                     /locus_tag="PLW82_02860"
     CDS             complement(2575..3558)
                     /gene="ispE"
                     /locus_tag="PLW82_02860"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOJ46246.1"
                     /translation="MSNSIQVNAKINLCLDVKSKREDGYHEIESLFHSIGVSDTLTIK
                     KADKKTLVSNNRQMPLNEKNLVLKAITKLEEELGTELNCAFNLEKRIPISAGLGGGSA
                     DAAGALILANHLFDLRLSLEELAGVAAKVGADVPFMLYGGSCLVSGIGDVIERSTPAP
                     SWGVLLIKPPFGIRTKSAYDLLDASEDLLHFDPTKLEQAIERRNYHEAKKNLGNTFTK
                     PIVEQCPSLSIIMETLDELGIGAVSLSGSGPTLFALTEDSGIIEELEAKLEKKIPNWQ
                     WTENRLPYRAERRSEPGDFRPLVIRGRDTKPWIHFARLTDFSWQIENASSL"
     gene            complement(3595..4254)
                     /locus_tag="PLW82_02865"
     CDS             complement(3595..4254)
                     /locus_tag="PLW82_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46247.1"
                     /translation="MDERERQRRELTKERELELFYTKTSPKRLRLAGLISGVLMPWIL
                     AAIVLLSLVVVNLYDYMMWRQWEFDPSDLFNETKVGLIVVTIILGVIGFIAAGAFPME
                     KVRFFKTDIYFSRPFSYFVIGFFGLLVPFLVIMWIMDGDFSILGFLSVFVISFVVALV
                     AYPYWKALFNKIIYYVSDYDAVGQVVQEMIEARAKQNVSRQEQEETYRRFLDEMRKHR
                     K"
     gene            complement(4269..>4690)
                     /locus_tag="PLW82_02870"
     CDS             complement(4269..>4690)
                     /locus_tag="PLW82_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOJ46248.1"
                     /translation="VKQGGTIINGDRVLEAATKNLTKVVYFGKLQDLLDIQLIWLELD
                     PTKNVVLSSDFTLDLTHQNATKGFAAKHLVESCGLKMEEVLAIGDNYNDMDLLEEAGF
                     SVAMADAPPEVRQVADAVTGRAAEGGFAEAIYKYVLG"
BASE COUNT         1083 a         1187 c         1061 g         1359 t
ORIGIN      
        1 atggcaagat caacactctg aactaactct tttatatctg cgaagcattc ggtgaactca
       61 tatgtaccgg tttctgcatt gtaggctgct ttgatttgag ggagatgtac cataatatcg
      121 ccagcagcag ctaaggtgat tctaatcggc tcgggctgat cttcttcagg aggtggttct
      181 gcagccacat tttcgttatg gatggtgctc ccaaaggtgg ccgtatacct tgaataaagc
      241 tttttgctaa atccgaacag tccgtccaag agaatcaaag ttaagactgc gatgatcaag
      301 atagataggg ttcttttctg catgatgttc acctcgaacc tcgtctgcta tgtgaagaaa
      361 gcttgtccct acaagggtgc aggaagcttt taaaaagcgg caggtttaaa ctgggtttaa
      421 accaagaaag tcgccagatg agctaacact atctacaggt tatccacaga actctcgaac
      481 cctgctaaac tgcgactaaa aaaggagcgc ataagcgctc ctatagtagg cattaagtat
      541 gccggtttga tgtacttagg catctaattc agccagcttc tcgagtacta atttcgtaag
      601 ctgctctctc atgtcagcgg taatcggatg ggcaacatct ctatactctc cgtttggcag
      661 cttacggctt ggcatggcta aaaaccttcc gtcgtcaccc tctatcactc tgatgtccct
      721 tactacaaac acatcattga aaacaactga ggcgatagct ttcatccgcc cagcttcatc
      781 aactctgcga acttgtacat cggtgatatg caccctttta ttccccctga gctgggttgc
      841 tgcacatagt taattattta acataagtcc gttttttcct tctgttttta tgccattttt
      901 gtcgatttcg acgcgttttt tggcttttgt gcgagaggga aaagagaaca ctgagaccgg
      961 gtgccgcttc ttgaatgcaa aaaagctgcc acgaggcagc ttcaaactca atgcgtctct
     1021 gcaaacaggt tttccattaa gtaaaggtcg ctcggtttat caacatcgat gccgatctcc
     1081 gggtaggtgc tgatgatagc tttggcttta aatcccagaa tctcttcggc tctctgctct
     1141 atttcttcta tggagagtga cttggtaatg aacttcacca ggaacctcag acctaagagc
     1201 ttcacgattt tcgcagggct ctttctcagg tctgtgagct gcctgatgag ctcaaagtgg
     1261 gtctctacaa cgtttggatc gaggagcatg atgtttccgc ctgtgaaagt gccttctttc
     1321 aaggggaagt atgttctctg tacttcggga taggaagctt cgttgacttc tctagagacc
     1381 agtggataaa aggccttagc gtccctatgt ttcaggcact gatcgataaa gtcgttgact
     1441 gcctcggtgc tgatcagcgg aatgtcgctg gttacgatca gtgtgtaagg tgatttgtca
     1501 gctccaagtg cgtcgatccc tgctttgagg ttgtcaacca gtgagtcttt gcagagggta
     1561 aacgaaatgc ttgggctggt ttctctgagt tcttcaggcc ccacaagcac aactttcccc
     1621 acgttttcaa gggttaacat ggtatcgatg acgtactcaa ccatcggccg actggcaact
     1681 gtgaccagag cttcccatgt agcactgcta acttctgtca aggaaccccg gtttggagcc
     1741 ccagctagga ttacaacgtc aaatttagtc acttttgcgg cgacagtctg tcgccacacc
     1801 tccctttccc ttgacaagaa tagagttgga atcaccttag ggacattata acttactttt
     1861 cccctctttg aaatatcgct tccatcaggc tgctctcggc attggcgatg tgctcttctg
     1921 ccagcttttt ggccagcttg gagtctcttt tgccgatagc ttctacccgc ttgcggtgct
     1981 ccacaaaggc ctgcttcatg cgccctggat gggcaagaga cgttgacctg tacctctgga
     2041 tgtgctctct gagattgttg atcatctcct caaggcggtc gtttcccgcg gctttgtaca
     2101 gtacatcgtg gaacatctca tctatctcta tagtctgttc aatgtcttct ctgtcaattg
     2161 cttcgccaat ccttaccaag tagcgctcga gcttttccaa ctgctcgtcg gtggctctga
     2221 gagctgcaag ctgggctgcc agaccttcga gggctctgcg gatttcaaag acctctttaa
     2281 catcgctttt ggtaagatcc gcaacgtagg cgcctttcct cggtaccata atcaccagtt
     2341 tttccagttc cagctttcta atggcctctc ttaccggcgt gcggctcacg ccgagctctt
     2401 cggccagcgc ttcctccatc agtctttcgc cagggcgcag cgtgccgttg ataatggctt
     2461 cgcgtatagc ttcaaaaacc aactctcgca aaggttgata attatctaat tggataggtc
     2521 ctaattttcc cataagctct tgaaagcact aatgtgcctc ctaaatgcct cctttcacag
     2581 tgaagatgca ttctctattt gccaactaaa gtctgttaat ctcgcaaaat gaatccaggg
     2641 ttttgtatcc cggcctctaa tgacaagtgg cctaaagtct ccaggttcag atcttctctc
     2701 tgctctatat ggcaacctgt tttcggtcca ctgccagtta gggatttttt tctccagttt
     2761 ggcttctaat tcttctatta tgccagagtc ttctgtcagt gcaaagaggg ttggaccgct
     2821 tcctgaaaga cttacagctc ctatacccag ctcatctagg gtttccatga tgatgctaag
     2881 agatgggcat tgctccacaa tgggttttgt gaaggtgttt cccaggtttt tctttgcttc
     2941 atggtagttt ctacgttcaa ttgcctgttc tagtttcgtc ggatcaaagt gaagcaggtc
     3001 ttctgatgca tctagcagat cgtaggctga tttggttctg atgccaaaag ggggcttgat
     3061 aagcaaaact ccccaggagg gtgccggtgt cgacctttcg atgacatctc caatcccgga
     3121 gacgaggcac gatccgccgt agagcatgaa gggtacatct gcccctacct tcgctgccac
     3181 acctgccagc tcttcgagag acagcctcaa gtcgaagagg tggtttgcca ggatcagggc
     3241 acctgctgca tcagcagatc ctcctccaag ccctgcagat atggggatcc gtttctccaa
     3301 gttgaaagcg cagttcagtt ctgtgcctag ctcttcctcc agtttggtta tagcttttag
     3361 caccaggttt ttttcgttta acggcatctg cctgttgttt gagaccagcg tttttttatc
     3421 tgctttctta attgttaatg tatcggaaac tccgatggaa tgaaataggg attcgatctc
     3481 atgataccca tcttccctct tgcttttcac atccaaacag aggttgattt tggcattgac
     3541 ctgtattgag ttagacatag ataacccttt cttcaaagca agtgcgccct gcgcttactt
     3601 gcggtgtttt cgcatctcgt ctaaaaagcg ccggtaggtc tcttcctgct cctgtcttga
     3661 gacgttttgc ttagctctag cttcaatcat ctcctggacg acctgaccga cggcatcgta
     3721 gtcgcttacg tagtaaatga tcttgttgaa cagcgccttc cagtacgggt aggccaccag
     3781 tgcaaccaca aaactgatga caaagacgct caaaaaacca aggatcgaga aatcgccgtc
     3841 catgatccac attataacca aaaacggtac aagcaggcca aaaaagccga tgacaaagta
     3901 gctgaatggc ctggaaaagt agatatccgt tttgaaaaac ctcacctttt ccatggggaa
     3961 agctccggcg gcaataaagc caattacgcc caggatgata gttacaacta taagtcccac
     4021 tttggtctcg ttgaaaagat cgctaggatc aaactcccac tgccgccaca tcatgtagtc
     4081 gtataggttt accacaacaa gcgacaggag gacgatagct gccaagatcc agggcatgag
     4141 cactccggag attaggcctg cgagcctgag tctttttgga gaagtcttgg tatagaagag
     4201 ctcaagctct ctctcttttg tcagctctct tctctgtctt tccctctcat ccatgaagac
     4261 acacctctct agcctaagac gtatttgtag atggcttctg caaagccgcc ttctgcggct
     4321 ctgccggtta cggcatcggc tacctggcgc acctcaggtg gtgcatcggc catggctaca
     4381 gaaaagcccg cttcctccag aaggtccatg tcgttatagt tatcgccaat tgccagcact
     4441 tcttccattt tcagcccgca gctttccacc aggtgctttg cggcaaaacc ctttgtggca
     4501 ttttggtggg tgaggtcgag agtaaagtcg cttgaaagaa ccacgttttt tgttgggtcg
     4561 agttctaacc atatcaactg aatgtccaaa agatcctgga gcttgccgaa gtagaccact
     4621 ttggtcaggt tctttgttgc tgcctctagc accctatctc cattgatgat ggttcctccc
     4681 tgctttacca
//
LOCUS       DAOSDA010000083         4667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11601_length_4667_cov_3.365351, whole genome shotgun sequence.
ACCESSION   DAOSDA010000083 DAOSDA010000000
VERSION     DAOSDA010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11601_length_4667_cov_3.365351"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLW82_02875"
     CDS             <1..433
                     /locus_tag="PLW82_02875"
                     /inference="COORDINATES: protein motif:HMM:NF026064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOJ46249.1"
                     /translation="ETGTFEQTVNEIRTKVAFATYAPITLISAVTKQRVLKLLEMIQE
                     VDAEARKLVPTSRVNEIIRDAIRMQEPPSDKGVRLRIYYGLQTGTQPPRFTLFVNDPN
                     LMHFSYERYLENRFREAFGFAGSRVLLSYKARTDTEAYDKR"
     gene            453..1049
                     /gene="plsY"
                     /locus_tag="PLW82_02880"
     CDS             453..1049
                     /gene="plsY"
                     /locus_tag="PLW82_02880"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOJ46250.1"
                     /translation="MLVVLSALIGYLVGSIPFGLLLCKYVFGIDIRQYGSGNIGATNV
                     QRNTNTLYGFVVLTLDLLKGFLAAWIGYALAGHNGAILGGITAVVGHNWSLYLKFSGG
                     KGVATTAGMALFLFPKGFLVLVPLWVLMVALTKYVSLASVTAALAMPIVVYFFGYPPK
                     LVAISVLAAAFAIYRHKSNIERLLNKTESKFSLKKKSS"
     gene            1199..1798
                     /locus_tag="PLW82_02885"
     CDS             1199..1798
                     /locus_tag="PLW82_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HOJ46251.1"
                     /translation="MAKVLYIKANPKQASNTFLISDSFIKAYKEKNPADQITTLDLYR
                     EGIDFLRERDLGDMGDPEKLEDRNHRVFKYAYQFLEGDKYVIAAPFWNLSFPAILKAY
                     IDYICISSLTFKYTEEGPVGLLAGRKAIYITTRGGNYSSEKTRALDQGEPYLKTILGF
                     LGITDFTVLSADCLDVIGVDKDEILRAAIREAENLAQKF"
     gene            1972..3453
                     /gene="spoIVA"
                     /locus_tag="PLW82_02890"
     CDS             1972..3453
                     /gene="spoIVA"
                     /locus_tag="PLW82_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HOJ46252.1"
                     /translation="MEATAVFRDIVARTRGDIYIGVVGPVRTGKSTFIKRFVDLMVLP
                     LIKDDQLKKRTVDELPQSGSGRMIMTTEPKFVPDEAVTIELQEGMKASLRLVDCVGYT
                     VTGALGYFDDEGLRLVRTPWSEEEISFEKAAEIGTRKVIQDHSNIGLVVTTDGSITDL
                     PRESYIDAEERVIRELKELGKPFIVLLNSTVPRDEKTIALAEELSARYEVSVVPVDCL
                     NLTQPQIDAIISSLLLEFPLREIAVDVPSWVSELSYDHKLREKLEEAIFEHIAGISKA
                     RQVGWSKEKILADVSELESVVTRELDLGRGRARIELNVKAEVFYEVLEEITGFAVEDE
                     ASLFSLLIELGSSKKEFDKVAAAMQEVRSKGYGIVRPGKEDIVLEPPESVKQGNRFGI
                     RLRASAPSYHIIKADIYAEVTPFVGTDKQGEEFVSQLESEFSENPSAIWEKDFLGKNL
                     SEMMLGGIEGKLYRMPFNAQEKLQETLERIINDGSGGLICIIF"
     gene            3611..3886
                     /locus_tag="PLW82_02895"
     CDS             3611..3886
                     /locus_tag="PLW82_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOJ46253.1"
                     /translation="MTKGELVGNIAEKAGVSKKDAEKVLNAVLSSITEALASGRKVQL
                     VGFGTFEVRHREARTGRNPRDPGKTIHIPSRDVPAFKPGKALKEAVK"
     gene            4182..>4667
                     /locus_tag="PLW82_02900"
     CDS             4182..>4667
                     /locus_tag="PLW82_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOJ46254.1"
                     /translation="MKEARMGVAVLGLGTVGIGVVDILSKNRAMIFNRSGVDLEVRKV
                     LVSNPNKKREIDLAKDMFAKDIEEILQDDEIDVVVELIGGLSPAKEYIIKALEAKKAV
                     VTANKAVLAHHAAELFAVARKNNVPLYYEASVGGGIPLITPLTESLVGSNITSVMGII
                     NG"
BASE COUNT         1281 a         1074 c         1227 g         1085 t
ORIGIN      
        1 agagacaggc acctttgaac agactgtaaa cgagatcaga acgaaagtag cgtttgcaac
       61 atatgctccc attacgctga tctcggcagt aaccaaacag agagttctca aactcttaga
      121 gatgattcaa gaagtggatg cggaggccag gaagctggtt cccaccagca gagtcaatga
      181 gatcatccgc gatgccatta ggatgcagga gcccccatcg gacaaaggtg tcagactcag
      241 aatctactac ggcctgcaga caggtactca accaccgagg tttactctgt ttgtcaacga
      301 tccaaatctt atgcattttt cctacgaaag gtatttggag aaccgcttca gagaagcctt
      361 tggttttgca ggctcgagag ttttgctttc atacaaagct cgaactgata ccgaagctta
      421 tgataagcga tgaaagggga agcatatagc agatgctggt tgtcctcagc gcactaattg
      481 gctatctcgt gggttcgata ccctttggtc ttctactctg caagtacgtt tttgggatcg
      541 atatcaggca gtacggaagc ggcaatatag gcgcaaccaa cgtgcaaagg aatacaaaca
      601 ctctctacgg tttcgttgtg ttaactctag accttttaaa gggtttttta gcggcctgga
      661 taggctatgc ccttgccggc cataacggtg ctattttagg cggtataact gcggttgttg
      721 ggcacaactg gtcgctgtac ttgaagtttt caggaggcaa aggcgtcgcc accactgcgg
      781 gaatggctct gttcctgttt cccaagggct ttttggtgct ggtgccactg tgggtattaa
      841 tggttgccct caccaagtac gtgtctctgg cgtctgtaac agcggccctg gccatgccga
      901 tcgtggtgta ctttttcggc tatccgccaa agcttgttgc aatcagcgtt ctggctgcag
      961 cttttgccat ctacaggcat aagagcaaca tcgaaaggct gttgaacaaa accgaatcga
     1021 agttcagctt gaaaaagaaa tccagctaat tcaaaggcca ccttaagttc aggatggcct
     1081 ttttttttgc cataaaggcc attggggact ttttattaaa gtctttgaat gtgtgagagc
     1141 cctgttagta ctggcccgta ttgggcaccc taaagggaga ctggaaggag aagtggcaat
     1201 ggcaaaggtt ctctacatta aggctaatcc caaacaggcc tcaaacacgt ttctcatctc
     1261 agacagcttc ataaaagcgt acaaagagaa aaatcctgca gaccaaatca ccaccctaga
     1321 tctctacagg gaaggtatcg attttctcag ggagagggat cttggtgata tgggcgatcc
     1381 ggaaaagctc gaagacagaa accacagggt cttcaagtac gcgtatcagt ttttggaggg
     1441 ggacaagtac gtcattgcgg cccccttttg gaatttgagc tttcctgcga ttctcaaagc
     1501 ttacatcgac tacatctgca tctcaagcct gacgtttaag tacaccgaag aaggtcctgt
     1561 aggtctcctt gcagggagga aggccatcta cattaccacc agaggaggga actactcgtc
     1621 cgaaaagaca agagcccttg accaagggga gccctacctc aaaaccatac tgggctttct
     1681 ggggatcacc gactttacgg tgctcagcgc agactgcctc gatgtaatcg gcgtagacaa
     1741 agatgaaatc ctccgagcgg caataaggga agcagaaaac ctcgcgcaga aattttagct
     1801 tcttgaaagc caaggggtct tactttctta cctaagtaat ataaatagtc atcagatgtt
     1861 ttatctgagg actatttttt tattgggaag ttctgttctg cttgtatcat gttctgcata
     1921 acttttaaga gagaggcctt tgttgataac ttcttaggga gggaagaaaa gatggaagcc
     1981 acagctgtgt ttcgagacat cgtagcccgc acccggggcg atatctatat cggagttgtc
     2041 ggcccggttc gcaccggaaa atcaacgttc atcaagagat tcgtggatct gatggttctg
     2101 cctttgatca aagacgacca gctgaaaaag cgtaccgttg acgaactgcc ccagagtggc
     2161 tccggcagga tgatcatgac cacagagccc aagttcgtgc ctgacgaagc ggtaaccata
     2221 gaactccagg aggggatgaa agccagcttg cgccttgtag actgcgtcgg ctacaccgtg
     2281 accggcgctc tgggctactt cgatgatgaa gggcttcggt tggttcgcac cccgtggagc
     2341 gaagaggaga tcagctttga aaaagcggca gagatcggca ctcgaaaagt catccaggac
     2401 cactccaata ttggcctagt ggtgacaaca gacggttcca tcaccgacct gccgcgggaa
     2461 agctacatcg atgcagaaga gcgggtaatt cgggagttaa aggaactagg taagccgttc
     2521 atcgtgctcc ttaactccac ggtccccaga gacgaaaaaa ccattgccct tgctgaggag
     2581 ctatctgctc gctacgaggt cagtgtggtg ccggtagact gcctcaacct cactcagccg
     2641 caaatcgacg ccatcatctc cagtctgctt ttggagttcc cgctcaggga gattgccgtg
     2701 gacgtcccca gctgggtgag cgaactttcc tacgaccaca agctcagaga gaagctggaa
     2761 gaagcgatct tcgagcacat cgccggcata agcaaagcca ggcaggtggg atggtccaaa
     2821 gaaaagattc tagcggatgt ttccgaactg gaatcggtag tcaccaggga gctggacctc
     2881 ggccgcggaa gggccagaat cgagctgaac gtcaaagcgg aagtgtttta tgaagtcctt
     2941 gaagagatca cgggctttgc tgtggaagac gaggctagcc tcttcagcct cttgatcgaa
     3001 ctgggatcct cgaagaaaga gttcgacaag gtggccgctg ccatgcagga ggtcagaagc
     3061 aaaggctacg gcatcgtcag gccaggaaaa gaagacattg tcttggagcc acctgagtca
     3121 gtgaagcagg gaaacaggtt cggcatcagg ctcagagcat cggcgccgtc gtaccacatc
     3181 atcaaagcag acatttatgc cgaagttact ccgtttgtgg gcaccgacaa acaaggtgag
     3241 gagtttgtca gccagctgga gtccgagttt tccgaaaacc catcggccat ttgggagaaa
     3301 gatttcttgg gcaagaacct ttcagagatg atgctcggag gcatcgaagg caagctctac
     3361 cgcatgccgt tcaacgctca ggagaagctg caggaaaccc tagagcgcat cataaacgac
     3421 ggcagtggag gcttaatctg catcattttc taggaagagc acaagctctt cctagttttt
     3481 tattggaaaa agaaggaaat attgtagttt atccgaattt ataaccaaga ccacctaaca
     3541 aggaggctac tagggacgtg agttccgagg ggttaggtaa aatttggagg tgaacgatat
     3601 gtccaaaggc atgactaagg gtgagttggt tgggaacata gcagaaaagg ctggggtatc
     3661 caagaaagat gcagaaaaag tattgaacgc tgtgttatca agcatcaccg aagctttggc
     3721 gagtggtaga aaggttcagt tagttggttt tggtactttc gaagtacgtc atcgcgaagc
     3781 tagaaccggc cgtaacccac gtgacccagg taaaactatt cacattccat cacgtgacgt
     3841 ccctgctttc aagcccggca aggctctgaa agaagccgta aagtagctcg aatagctgac
     3901 gaaaaagaga ggctgtccat aagttggtca gccctcttat atttttaaaa acctataggc
     3961 ggttttcgta cgttggagct tgcaggaaac gaaaaactgc agtctttttc tcgttaaaaa
     4021 ctggttttct cgtgcaactc gggcaatttt ctacgaatcc gtttaccaaa tcccttcttc
     4081 atggtagaat aggaaaaacg ttaaattttt gctaagaata tcacaaagga accatagagg
     4141 aaaggtggtg gggacagcac cgtcggtgct gtccgggcaa attgaaagaa gcaagaatgg
     4201 gagtagccgt acttggactt ggaacagtcg ggatcggcgt cgtagacatt ctctccaaaa
     4261 atcgggcgat gatcttcaac cgcagcggcg tggacttgga agtaagaaag gtcctggtga
     4321 gcaatcccaa taaaaagcgg gagatcgatc tagccaaaga catgttcgcc aaagatatcg
     4381 aagagatact ccaagacgac gaaatcgacg tggttgtgga gttaataggc gggctttctc
     4441 ctgccaaaga atacatcatt aaggcattag aagcgaaaaa ggcggttgtt accgccaaca
     4501 aagcggttct tgcccaccac gcagcagaac tgttcgctgt tgccagaaaa aacaatgtcc
     4561 ctctgtacta tgaggccagt gttggcggag gaattccgct gattaccccc ctcaccgagt
     4621 ccctcgtagg cagcaacatc accagcgtca tggggatcat caacggt
//
LOCUS       DAOSDA010000084         4624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11750_length_4624_cov_2.855329, whole genome shotgun sequence.
ACCESSION   DAOSDA010000084 DAOSDA010000000
VERSION     DAOSDA010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11750_length_4624_cov_2.855329"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLW82_02905"
     CDS             <1..840
                     /locus_tag="PLW82_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ46255.1"
                     /translation="EGLEIVLSTAWGADKGAAATISGQVDIGFFGPEAAIYIYNQGAK
                     DYLIAFAQLTAMDGSFLVARDMEKEFSWADIAGKTVIGGRPGGVPQMTLEYVLKKYGY
                     TLKGDVDLVTNLAFTATSGAFESGMGDYIALFEPTATLLELNGKGKIVASLGLEGGPL
                     PYTVYHARKDYFEKNPDIIQRFTNAILKGQLWVATHSAEEIAEVIYSYFPEIEKDHLV
                     AVVDRYKAQNSWNPSLLLSRDAFERLLTIMIEAGELDRRVPYEAIVNNKHAIKAIETV
                     TLD"
     gene            852..1133
                     /locus_tag="PLW82_02910"
     CDS             852..1133
                     /locus_tag="PLW82_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ46256.1"
                     /translation="MAKVSLQDVSLAYYGQNGATEAVQNVSINIEEREFVALVGPSGC
                     GKSSVLSMVAGLVRPTSGKIFLDGEEVTKPSSKIGYMLQQDHLFRQMQI"
     gene            complement(1130..2245)
                     /locus_tag="PLW82_02915"
     CDS             complement(1130..2245)
                     /locus_tag="PLW82_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOJ46257.1"
                     /translation="MKVLLYARVSTEAQTQKHSLDAQLNALWEFAKEMGFEVAGEYVD
                     RGISGRSDEREAFLAMIRHATTPGANIGAILVHKLDRFSRNREDAIVYKALLKKHGVS
                     VLSSTEPVEDSPAGLLVEGILETIAEFYSRNLAQEVKKGQTEAAKKGQNQSEPPLGYR
                     SVGGRLSVKPAEAEIVKFIFERYLQGISYRKIAGEVEAKFHKSLHPSTIAYILRNEAY
                     IGIRIWNKRQKDGSIRPKSDWVVKEQSHTAIIEKDLFFKVQKLLKQRSKPKSEHLLSG
                     LCQCMICNSPLHSQMQRGKVRLRCPEKLCPMGSVAEEKVAEAVFTDLELLLHHASPLG
                     KPDLSGCNQEVRRLVLLSSIEKILIGRTALEIHYLSY"
     gene            2327..3154
                     /locus_tag="PLW82_02920"
     CDS             2327..3154
                     /locus_tag="PLW82_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46258.1"
                     /translation="MLKAVIDLGSRAARILVADVQRSSFEVHYSRGYLTLLGQGIDVL
                     SLKRLNQALRHFANITQKMNISPENVLAFGTEVFRQNPGLKKQVTRYFPGFEIISGEE
                     EAIYSFAAGTLSQKNLEENQRVLVIDQGGGSLELSLGYRFEDVLVVERAASFPRYGTF
                     NFPVDYRSFSEELVLNFPTKEELEEQRPRGLQSIIGMGSVVTQLAFVLSARSEYRIEE
                     VNGFVIHREDLTRRVRKQQSLRAFVDVLDHYGFDQMQVSGWGTRHGALFSDRILVRK"
     gene            3319..3828
                     /locus_tag="PLW82_02925"
                     /pseudo
     CDS             3319..3828
                     /locus_tag="PLW82_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            3815..>4624
                     /locus_tag="PLW82_02930"
     CDS             3815..>4624
                     /locus_tag="PLW82_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ46259.1"
                     /translation="MFMNETAVKSAGLIAYERAMRRRAVLKTAAQILLLALLIALWEV
                     GVQKGWLNGFIFSSPSKIWLQLAKMIRSGELFYHLWVTTYETVIGFSLGTLIGVVVAV
                     VLWLFPFVSKVLDPYLIVLNSIPKVALGPIFIVWMGSGTNAIITMALAISVIVTIMMV
                     HTGFKETDPDKIKLLQSFGATRVQVLKKVVLPSALPSIIGALKVNVGLSLVGIITGEF
                     LVSQAGLGFLIVYGGQVFQLTLVMTSIIVLMVFAALLNVLVNFLEKFLFKNL"
BASE COUNT         1121 a         1067 c         1253 g         1183 t
ORIGIN      
        1 gagggtttgg agattgttct ttcgaccgca tggggtgccg acaaaggagc agctgcaact
       61 atttctggcc aggtagacat cggcttcttt ggacctgaag cagctattta catctacaat
      121 cagggtgcca aagactatct gattgccttt gcccagctga ctgctatgga cggttcgttc
      181 ctggttgcaa gggacatgga aaaggagttt agttgggccg atattgctgg caaaaccgtt
      241 attggcggta gacccggcgg cgtgccccag atgactttgg agtacgttct gaagaagtac
      301 ggttacaccc tcaagggaga tgtggacctg gtaaccaact tggccttcac ggcaacttca
      361 ggtgcttttg aatcaggcat gggcgactac attgccctat ttgagccaac agctacactt
      421 cttgagctaa acggcaaggg caagatcgtc gcttctctgg gcttagaagg cggtccattg
      481 ccttacactg tctatcacgc tcgtaaagac tactttgaga agaaccccga tatcatccag
      541 cgttttacca acgctatctt gaaaggccag ctttgggttg ccacccattc cgctgaggaa
      601 attgctgagg tgatctacag ctacttcccg gagatcgaaa aggatcacct ggtggccgtt
      661 gtagatagat ataaagccca aaacagctgg aaccccagtc tgctgctcag cagagatgca
      721 ttcgagcggt tattgaccat catgatcgaa gcaggagagc tcgatcgtag agtgccgtat
      781 gaggcgattg taaacaacaa gcatgccatc aaagccatcg agacagtgac cctcgattag
      841 gagggatggc gttggcaaag gtttcgctac aggacgtaag tctggcctac tacggtcaaa
      901 acggagctac tgaagcggta cagaacgtga gcatcaacat tgaggaaaga gagtttgtgg
      961 cgctggtcgg tcccagtggc tgcgggaaga gcagcgtgct gtcgatggta gcgggcttgg
     1021 ttcggcctac ctcgggaaag atctttttgg acggggagga agtcaccaag ccctcgtcca
     1081 aaatcggcta catgttgcaa caggaccacc tctttagaca gatgcagatc tagtaggaaa
     1141 ggtagtggat ttccaaagcg gtccgtccta tcagaatctt ttcaatgctg ctgaggagaa
     1201 caagcctcct aacctcctgg ttgcagccgg aaagatcggg ttttcccagt ggtgatgcgt
     1261 ggtgaagcag aagctctaag tctgtgaaaa ccgcctctgc tactttttct tctgcaacag
     1321 agcccatggg acagagcttt tccggacacc gaagccgaac tttccctctt tgcatttgcg
     1381 aatgcagagg gctgttgcag atcatgcact ggcaaagtcc cgacaaaagg tgctcgctct
     1441 taggcttaga cctttgtttg agaagctttt ggactttgaa gaaaaggtct ttttcgatta
     1501 tagcggtgtg gctttgttct ttcacaaccc aatcggactt aggccttata cttccgtctt
     1561 tctgccgttt gttccaaatg cggatgccga tgtatgcttc gtttctcagg atgtacgcaa
     1621 tagttgaggg atgcaaactt ttatggaatt tagcttctac ctctccggcg attttgcggt
     1681 aagagattcc ctggaggtag cgttcgaaga tgaacttcac gatctctgct tcagcaggtt
     1741 ttacactcaa tctgccgccg acagaccggt accccagcgg tggctccgac tggttctggc
     1801 cttttttcgc agcttccgtt tggccctttt ttacttcctg agccaggttc ctagaataga
     1861 attccgctat ggtttcaagg atgccctcaa ctagaagccc tgccggcgag tcttcaacag
     1921 gttcggtgct gctgaggacg ctcacgccgt gctttttcag caaggccttg tagactatcg
     1981 cgtcttcacg gttgcggctg aagcggtcga gtttgtggac gagaattgcc ccgatgttcg
     2041 ccccgggggt tgttgcatgg cgaatcatgg caagaaacgc ttctctttcg tcggatcgcc
     2101 cggaaatccc cctgtctacg tattcgccgg caacttcaaa gcccatctct ttggcaaact
     2161 cccacaacgc attaagttgt gcatcgaggg aatgcttctg cgtctgggct tcagtagaga
     2221 ctcttgcata aagcagaacc ttcacgcaaa tcacccctta gagcatatga aacatgcagg
     2281 aaatgagaac taataatcta aataagaaat gaaatggggt ggttgtgtgc tgaaggcagt
     2341 tatcgatcta ggaagcagag cagctcgcat tcttgtagcg gacgtccaac gcagctcttt
     2401 tgaagtgcat tactcccgcg gttaccttac tctactgggt cagggaatcg acgttctttc
     2461 actgaaaaga cttaatcaag ctctgagaca cttcgcaaac atcacccaga aaatgaacat
     2521 ttcccctgag aacgtgctgg ccttcggcac ggaagtgttc aggcaaaacc cgggcttgaa
     2581 aaaacaggtc actcgctact ttccagggtt cgagatcatt tccggtgagg aggaagcgat
     2641 ctacagcttt gccgcaggga ccctgtctca aaaaaacctc gaagaaaacc agcgggtgct
     2701 ggtgatcgac cagggtggag gcagcttgga gctcagtctg ggctaccggt ttgaggacgt
     2761 tcttgttgtg gagagggcgg caagctttcc gcgttacggc acgtttaact tccctgtcga
     2821 ctacaggagc ttttccgaag agttggtctt gaacttccct acaaaggagg agcttgaaga
     2881 gcaaagacct cgaggtctac agagcattat aggaatgggt tctgtggtca cgcagctggc
     2941 ttttgtcttg agcgcccgct cggagtacag gatcgaggag gtcaacggct ttgtgataca
     3001 tagagaagac cttaccaggc gcgtgcgcaa acagcagagc ttgcgggctt tcgtcgatgt
     3061 gctggaccac tacggatttg accaaatgca ggtaagcggt tggggaacca gacatggtgc
     3121 actgttcagc gatcggatct tggtcagaaa atagaagctt tctgaaacct ctcttggcat
     3181 agacttaaag ggaggttttt ttatggaggt aaagtctaaa tatcctaaga ccgttgaacc
     3241 ccttctactt aggttggtgc agatactaaa ctcagggaag aaaaacgcgt cggcagaaaa
     3301 gatcagtcgt cgaaagagct ccacctcttt ccatggcgaa ccatctggca gaacgccacg
     3361 ttgggattag aagtggctgg aataaaaggc aggcatgagg ccgtacgtga gatgctggta
     3421 aaatatggcc tcggtggttt tgaacaccac tatccccgag aactctcagg agggatgcgt
     3481 cagagggtgg cattgattag gactttggct atcgaccctg aggtgctgct cttagacgaa
     3541 cctttttcag cactggatta tcaaaccagg ctcatgttgg aagaagaagt tgcagcgatc
     3601 ttgaggcagg agaaaaaaac ggttcttctg gttactcacg acatctccga ggcggttgcc
     3661 atgagcgata gggtcatcgt gctttccaaa cgcccgggaa tggtcaaaaa ggagttcaag
     3721 atagagttgg gaggaacttt cgacgatccg gattttagac ccagggcatc caagaggttt
     3781 ggcgagttct tccagttgat ttggcaggag ctggatgttt atgaatgaaa ctgcagtaaa
     3841 aagcgccggg ctgatcgcct acgaaagggc tatgaggaga cgggcagtgc ttaagactgc
     3901 agctcagata ctgctcttag ctctgctgat cgccctatgg gaagtgggag ttcagaaggg
     3961 atggctgaac ggatttatct tttcatcgcc ttcgaagatc tggttacagc ttgcgaaaat
     4021 gatcaggtcg ggagagctct tctaccacct gtgggtcaca acctacgaga cggtgatcgg
     4081 cttcagtttg ggaactctta tcggtgtcgt tgtcgcagtc gtactctggc tgtttccttt
     4141 tgtcagcaag gtgttggatc cgtatctaat cgttctaaac agcataccta aagtcgcttt
     4201 aggaccgatc tttatcgtgt ggatgggaag cggaaccaac gcgataatca ccatggccct
     4261 agccatctcg gttattgtga cgatcatgat ggttcatacc gggttcaaag aaaccgatcc
     4321 agacaagatc aagctattgc agagcttcgg cgcaaccaga gttcaggtgc tgaaaaaagt
     4381 cgttcttccg tcggctttgc cgtcaattat aggagcactg aaggttaacg ttgggctttc
     4441 tcttgtgggt attatcacag gtgagtttct ggtttcccag gcaggactgg ggtttttgat
     4501 agtctacgga ggtcaggttt ttcaactgac tttagtgatg acttcgatca tcgtgctgat
     4561 ggtatttgct gcactcctca atgtgctggt gaatttcctg gagaagtttc tcttcaaaaa
     4621 tttg
//
LOCUS       DAOSDA010000085         4599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11844_length_4599_cov_3.102773, whole genome shotgun sequence.
ACCESSION   DAOSDA010000085 DAOSDA010000000
VERSION     DAOSDA010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11844_length_4599_cov_3.102773"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..802)
                     /locus_tag="PLW82_02935"
     CDS             complement(158..802)
                     /locus_tag="PLW82_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOJ46260.1"
                     /translation="MDLTVWGTFLSAFLIGFSGAMMPGPMLGVTISGSLRKGFIAGPL
                     VVLGHGILELLLVFAMAFGLSAFFENPTVAGLIGLIGGLFLAWMGYGMIKSGLQKTVS
                     LSKTQSERKWEKNLVLAGVLVSITNPYFIMWWATTGIESVRQAYTLGILGVLLFFVGH
                     ILSDLTWYSAVSFAMHKGKSLMSDKVYQGLIVVLGVFLLAFAVKFAVGGWQMLF"
     gene            1052..3688
                     /locus_tag="PLW82_02940"
     CDS             1052..3688
                     /locus_tag="PLW82_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HOJ46261.1"
                     /translation="MKKIVIGILAHVDAGKTTLSESMLYLSGQIRKLGRVDKKDSFLD
                     MHELERQRGITIFSKQTAFQVGDTQFTLLDTPGHVDFSAEMERTLQVLDYAILVISGA
                     DGVQAHTSTLWRLLTLHKIPTFVFVNKMDQIGAERESLLVQLKKQLDERCIPFEEAPT
                     ASFFEELALCDEGMMHAYLETNRIETGLIKRAIQGRKVFPCFFGSALKLDGVEPFLRS
                     LDLYTTAPVYGKEFGAKVFKISRDDQGNRLTHLKVTGGRLQVKSVLSNGQWEEKINQI
                     RIYSGEKYEVVGEVEPGMICAVTGLTQSKPGEGLGIEKDSSAPVLEPVLSYRVILPEG
                     CDHRAFLPKLQEIEDEHPELRIHWNSELQEIQAQIMGDIQIEILKSLIHTRFDVEVQF
                     DTGRILYKETIANTVEGVGHFEPLRHYAEVHLLLEPGKRGSGLLFASACSEEVLGKNW
                     QNLVLTHLKEKKHIGVLTGSEITDMKITLISGRAHNQHTQGGDFREATYRAVRQGLKE
                     AESILLEPYYSFRLEVPTASVGKAMTDVEQMHGTCEILEHGNEFSVLVGTAPVSTMRN
                     YHREVLAYTNGRGRLFCNLDGYQPCHNAQEVIAEIGYDSESDLENPTGSVFCANGTSF
                     LVSWNEVKTYMHLEPYFKAPHGRGDQSISKSPKKEQDSSTDDDYYYLGLVNPKQATKA
                     RELRRRPSREPMKDPLEAVSKEEKPKENYLLVDGYNIIYAWPELREQAENENMEGARM
                     GLLNALSNYQAIKNDHIIVVFDAYRTQRSTEDVVDYGNIRVVFTKEAQTADQYIEKFA
                     YDNQKKYNIKVATSDGLEQIIIRGMGSSLVTPNELKDEIDQTLERSEQAYRDKQGTTR
                     TSLKDALSEQALKHIEGLEESE"
     gene            complement(4175..>4599)
                     /locus_tag="PLW82_02945"
     CDS             complement(4175..>4599)
                     /locus_tag="PLW82_02945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOJ46262.1"
                     /translation="GVKRGEYGFSGGEWKPAIPFLLIYTAAFLFSGLTAERWLFLLYA
                     VLYSGFQEEFFYRGVMQPLFTAATKKPVWGIGLSVGLFALLHIPDFVFRVYPTVPMAL
                     SGVASTALFGGLMAYGAYRTGILWPWVLIHALSNVVGF"
BASE COUNT         1321 a         1168 c         1089 g         1021 t
ORIGIN      
        1 ctgcaatgta cagcctgaac ttgacgcagc ttcgcacaca acctcataaa agcaaaaaga
       61 ccgctatcgc ggtctttctt atgttcaaat agatgcgcaa gcgacaaacc gagataccgc
      121 tattccgtga gtaaagcgct cttgcggtat tagacttcta aaatagcatc tgccagccgc
      181 ctacggcaaa cttcacggca aaagccaaga ggaaaactcc taacacaacg atgagacctt
      241 gataaacctt gtcgctcata aggcttttac ccttgtgcat ggcaaaggac acggcactgt
      301 accaggtgag atccgaaagg atgtgcccca caaagaaaag cagaactcct aagatgccaa
      361 gggtgtaggc ctgtctcacc gactctatgc ctgtggtcgc ccaccacatg atgaagtagg
      421 ggttggtgat actcaccagg actcctgcaa gaaccagatt cttctcccac ttccgctcgc
      481 tctgggtttt cgagagggag acggtcttct gcagaccaga cttgatcatg ccataaccca
      541 tccaggccag gaacaacccg cctataaggc cgattaatcc ggcgacagtc gggttttcaa
      601 aaaaggcgct gaggccaaag gccatggcaa acaccagcag gagctccagg atgccgtggc
      661 cgagaaccac tagagggcct gcaataaaac ccttcctgag gctgccacta atggtgacgc
      721 caagcattgg tcctggcatc atggcccctg aaaagccgat caagaacgcg ctcaaaaatg
      781 tgccccaaac cgttagatcc atatcgaatc agtcactttc cgcagtttat tttccagtat
      841 atgatggatt tcggattctg tccagccgag taaagcgttt ctcggcggaa tgcgtcgttt
      901 ttctcaagaa atccgattct ttcctcttct gtaattactt gcctcagttt gcccacgcgt
      961 gaccgatcca agctgtggac cttcggtcga ttgtaagaac cttaagttac tggtatcatt
     1021 taaaggaatt cagcagaggg aatgaaccga gatgaaaaag atcgttattg gaatactagc
     1081 acacgttgat gctgggaaaa ccaccctttc agaaagcatg ctctatctga gcggccaaat
     1141 caggaaactg ggtagagtcg acaaaaaaga ctcctttttg gacatgcatg aactggaaag
     1201 acagcgaggg atcaccatct tctccaagca gacagcattt caagtgggag atactcagtt
     1261 cacgcttttg gacacccctg ggcatgtgga tttctcagct gaaatggagc gtactctgca
     1321 ggtcctagat tacgccattc tagttattag tggtgcagac ggagtccaag cccatacctc
     1381 tactttgtgg cggcttctta ccctacacaa gatacctaca ttcgtttttg taaacaaaat
     1441 ggatcagatc ggtgcagaaa gagagtccct tcttgtgcag ctgaaaaaac agcttgatga
     1501 aaggtgcatt ccttttgagg aagctccgac agccagcttc tttgaggagc ttgctctgtg
     1561 cgacgaaggt atgatgcacg cctatcttga aacaaaccgc atcgaaacag ggctgatcaa
     1621 gagggctatc caaggacgca aagtcttccc ctgtttcttt ggctctgcct taaagctaga
     1681 tggagttgaa ccctttctcc gcagccttga cctgtacaca acagctcctg tctatgggaa
     1741 ggagttcggt gcaaaggtct tcaaaatctc ccgagacgac cagggtaacc gcctcactca
     1801 cctgaaggtc acgggagggc gtttgcaggt gaagagcgtc ctctccaacg ggcaatggga
     1861 agaaaagatc aaccaaatcc gcatttactc aggtgaaaag tacgaagttg ttggtgaagt
     1921 agagccggga atgatctgcg ctgtaacggg tctgacccaa agcaaacccg gagaaggtct
     1981 cggtattgag aaagactcgt ccgctcctgt cttggagcca gtgctctctt accgggtaat
     2041 tctaccggaa ggctgcgacc acagagcttt tctacctaaa ctacaagaga tcgaggatga
     2101 gcatcccgag ctgagaatcc actggaacag tgagctgcag gagatccaag cccagatcat
     2161 gggcgacatt cagattgaga ttttgaagag cttgattcat acacgttttg atgttgaggt
     2221 gcagttcgat acaggaagaa tcctctataa ggaaaccatc gccaataccg tggaaggcgt
     2281 tggtcacttc gagcctttga ggcactacgc tgaagtccat ctccttctag aacctggaaa
     2341 acggggaagt ggtttactct ttgcaagcgc ctgcagcgaa gaggtcctgg ggaaaaactg
     2401 gcagaacctg gttcttacgc accttaaaga aaagaaacac ataggcgtcc tcacaggttc
     2461 ggagatcacc gatatgaaga tcactttgat ctccggccga gcccacaacc agcatacaca
     2521 aggcggagac ttcagagaag ctacttacag agcggtgcgg cagggtctga aagaagcaga
     2581 gtccatcctc cttgaaccct actactcctt ccgcctggag gtgccgacag catcggttgg
     2641 aaaagccatg accgacgtgg aacagatgca cggaacctgc gaaattctcg agcacggcaa
     2701 tgagttctca gtccttgtgg gcactgcccc tgtatctacc atgcgcaatt accacaggga
     2761 ggtgctggcc tataccaatg gacggggcag gctcttctgt aatttagatg gttaccagcc
     2821 ctgccacaat gcccaggagg ttatcgcgga gatcggctac gactcagaaa gcgatctgga
     2881 aaaccccaca ggttctgttt tctgcgcaaa cgggacaagc tttctggtga gttggaacga
     2941 ggtaaagacg tacatgcacc tcgagcctta tttcaaagct ccacacggca gaggagatca
     3001 gagcatctcg aagagtccca aaaaggagca ggatagttct accgacgacg attactacta
     3061 cttagggcta gtaaacccca agcaggcaac aaaagcccgg gagctaagac gacgaccttc
     3121 acgtgaaccg atgaaagatc ctttggaagc cgtttccaaa gaagaaaagc ccaaggaaaa
     3181 ctacctcctt gtcgatgggt acaacatcat ctatgcgtgg cccgaactta gagagcaagc
     3241 ggaaaacgaa aacatggaag gggcaagaat gggtctgctt aatgccctca gcaactacca
     3301 agcaataaaa aacgaccata ttattgtggt ctttgacgcc taccgcacgc agcgctctac
     3361 cgaagatgtt gttgactatg gcaacatccg cgtggtcttc actaaagaag cgcaaacagc
     3421 tgaccaatat attgagaagt ttgcttacga taatcagaag aagtacaaca tcaaagtagc
     3481 cacgtcagat ggcttggagc aaatcattat caggggtatg ggttctagtt tggtgactcc
     3541 aaatgaactc aaagacgaga tcgaccagac ccttgaacgc tccgagcaag cttatcgcga
     3601 taaacaaggc acgactcgca cttcccttaa ggatgcattg tcagaacaag cgctgaagca
     3661 tattgaggga ttggaagaaa gcgagtaaca cgctgtaagc aactccttta acttaaaccc
     3721 tagcatgagc tgccgaacta aaccctcttt ccttagatgc cggcttcccg acaatagcaa
     3781 ctactttacg cgtcaacttt ttgtatcatt cagaaaggat gcattccttc tttgaacgga
     3841 ggtttgcggt tggatgcgcc tacctttaag cagagtaagt ttgttgatca accgaaaaac
     3901 aacgcccaag tatgtgtaga gaccctatag gtgagtacct cgcttaatca cttaaaagcc
     3961 tgacagtgct tttgtcaaaa cctaatccaa ataagaatag cgagtcccct cttacgatga
     4021 ctttgtcaat ctaggcgaca acactaatgt ttgagcgacc attacaaaaa gccggtgtac
     4081 ccgttcagat acaccggctt ctcttatttt ctaccactta agttctccag atccacccat
     4141 tagcttggtc atctcttcga tccgcactaa caacctaaaa gcccaccaca ttacttaggg
     4201 catggatcag cacccaaggc cagaggattc cagtacggta tgctccatag gccatcaaac
     4261 ccccaaacag cgctgtactc gccacgccgc taagagccat gggaacagtg gggtaaactc
     4321 ggaaaacaaa atcggggatg tgaagtaggg cgaacaaacc taccgaaagg cctatccccc
     4381 aaacgggttt ctttgtggca gctgtaaaca aaggctgcat cacaccacgg taaaagaact
     4441 cttcttggaa tccagagtac aagacggcat acaaaagaaa cagccagcgt tccgcagtta
     4501 agccgctaaa aagaaatgcc gctgtgtaga taagcagaaa tggaatggca ggcttccatt
     4561 ctcctcctga gaatccatac tctccccgct ttactccta
//
LOCUS       DAOSDA010000086         4596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11855_length_4596_cov_3.781326, whole genome shotgun sequence.
ACCESSION   DAOSDA010000086 DAOSDA010000000
VERSION     DAOSDA010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11855_length_4596_cov_3.781326"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>973
                     /gene="hflX"
                     /locus_tag="PLW82_02950"
     CDS             <1..>973
                     /gene="hflX"
                     /locus_tag="PLW82_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOJ46263.1"
                     /translation="AVTVGRMIQNRPAPDTLTYFGEGKVQELVDFVVANEIDLIIADD
                     EISPVQVDQLSELSGVKVLDRTSLILDIFAQRARSKEGQLQVELAQLKYTLPRLKGSR
                     KNLSRLGGGIGTRGPGETKLEVDRRVLRHKIAQLRREIDEISKNREVQRKKRSENEVP
                     QVALVGYTNAGKSSLLKTLTGSDTYIEDQLFATLDPLVRRLELPNKIQILLSDTVGFI
                     RKLPHDLVAAFRATLEELKHASLLLHVVDSTGIKYEAQIKAVESTLKSIGCNTPTLLV
                     FSKADLLSAEERQFITTMYPKAVLCSSKTGEGLDVLLEEIIKTAKLGK"
     assembly_gap    974..983
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1153..1962
                     /locus_tag="PLW82_02955"
     CDS             1153..1962
                     /locus_tag="PLW82_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ46264.1"
                     /translation="MKVGCSSWSFHRTFAKKELTFKEWIRLCAEHYRLDGVELLAPHF
                     ESDEPEYLLEIKTLCTELGLTISCVSATNNFTVASQEELDREIEHVKGFVDVASILGA
                     PLVRIFAGVSSQLTDDEVWANVVAAIRECAEYALEHGIMLALENHGGFMDDQILRIIE
                     DVDMENFKVTMDLGNFGTDPNIIYKAMENVAPHTIFVHAKCMEFTEDYLEKTLDYKRI
                     MKILDDAGFNGFLSIEFEGPGEEKEEVGKAVAMLRNLTGAIHKEQVDKLVK"
     gene            2006..2908
                     /locus_tag="PLW82_02960"
     CDS             2006..2908
                     /locus_tag="PLW82_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOJ46265.1"
                     /translation="MGKFYYGGQAVMEGVMMRGPKTISIAVRKEDKSLIVHKEDVTSI
                     GERFPILKKPFIRGTVSLVESLVIGMKALTFSANQFASEDEQEELSPKEMVLTLGLSF
                     GLAILLFVVLPAFLTRLIGQYMQSTLLINFVEGLIKLVLFLLYIVGISFLSDIKRFFQ
                     YHGAEHKVINAYEAGEELTVENVRKHSRFHMRCGTNFITIVLILNIFIFTLVTGGARL
                     AFFPRVGAHLLLIPVVAGVSYEVLRFLGKEDAPNWAKALARPGLATQIITTREPDDSQ
                     IETAILALKEVMGADQSSNEKAIV"
     gene            2978..4045
                     /gene="prfA"
                     /locus_tag="PLW82_02965"
     CDS             2978..4045
                     /gene="prfA"
                     /locus_tag="PLW82_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOJ46266.1"
                     /translation="MLDKLEAIEQLYNELTEKMADPKLAQDRQEYQKVGKRRSELEEI
                     VEAYHRYKDIQNQIEGAKELLRETTDPELRELAEVELEELEETLASTESELKMLLLPK
                     DPNDEKNVIVEIRAGTGGDEAALFAGNLYRMYSHYAEGRRWKLEVLSGNATELGGFKE
                     IIFMIEGKGAYSRLKYESGVHRVQRVPETESGGRIHTSTATVAVLPEAEEVDVYIDPN
                     DIKIDVYRSSGPGGQSVNTTDSAVRITHIPTGLVVSCQDEKSQHKNRDKAMKILRARL
                     KEMAEMEARSKEAQERKSQIGTGDRSERIRTYNFPQGRVTDHRINLTLYRIEEILNGD
                     LDEIIDALSLADQEERLKEME"
     gene            4047..>4596
                     /locus_tag="PLW82_02970"
     CDS             4047..>4596
                     /locus_tag="PLW82_02970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HOJ46267.1"
                     /translation="MPQLLEILNSAGEYLQKKGSPSPRLDAEVLLADILGMQRIDLYA
                     KFDRPLDQKELALYRKAIARRANMEPVAYIIGRKEFYSREFTVTPDVLIPRPDTEVIV
                     EKVISYAKSFGIESPEILDLCTGSGAIAITLKLEIPSSQVTATEISEKALEIAWKNAA
                     KLGADVSFVQGDLYAGIDKQFDI"
BASE COUNT         1297 a         1088 c         1180 g         1021 t
ORIGIN      
        1 gcagtcactg ttggaagaat gattcaaaac cggcctgcac ctgacacact gacctacttc
       61 ggcgagggca aagtccagga gttggtagac tttgtggtgg caaacgaaat agacctcatc
      121 atagccgatg acgagatatc tcctgtacag gtcgatcagc tttcagagct ttcaggagtc
      181 aaagtgctag accgcacttc cctaatactg gacatcttcg cccagcgagc aaggtcaaaa
      241 gaaggccagc tccaggtgga acttgcccaa ctcaagtaca cgctgcccag acttaaaggc
      301 agtaggaaaa atctctccag gcttggcggc ggtatcggca ccagagggcc cggcgaaacc
      361 aaactagaag tagaccgcag ggtgctcaga cacaagatcg cccagctcag gcgggagatc
      421 gacgagatca gcaagaaccg cgaagtgcag agaaaaaagc gcagcgaaaa tgaggttcct
      481 caagtggctc tggtagggta tactaatgca ggcaagtcat cgcttcttaa aacccttaca
      541 ggatcggata cctatattga agaccagctc tttgctactt tggatcctct agtccgcaga
      601 cttgagcttc cgaacaagat ccaaatacta ctgagcgaca ccgtagggtt tatcaggaaa
      661 ctccctcacg atcttgtagc agcgtttagg gcaactctgg aagaactgaa gcatgcatcg
      721 ttgctcctgc acgttgtaga ttccaccggc atcaagtacg aagctcagat caaagctgta
      781 gaatccacgt tgaagtctat cggctgcaac acgccaactc tcctggtctt ctccaaggca
      841 gatttgctgt cagcagaaga gcggcagttc atcaccacta tgtacccaaa agcagtgctc
      901 tgttcatcaa agaccggaga aggtttagat gttttgcttg aagaaataat aaaaaccgcg
      961 aaactaggga aggnnnnnnn nnngctgaat ctgaagctag cgtacagcga tctccatctt
     1021 ttggaccgcc tgtataaaga cagtgaagta aaaaacgtag agtatcaaga agaaggttta
     1081 aaggtcactg ctgtagtcaa tatgtcttta gcagagagcc ttttgccgta tattgttgag
     1141 gaggaataag aaatgaaagt aggttgcagt tcctggtctt ttcaccgcac ttttgcgaaa
     1201 aaagagctca cttttaaaga gtggatccgt ctctgtgcag agcactaccg cttggacggc
     1261 gtagagcttt tggcacctca ttttgaatcg gacgaacctg agtacctctt ggagatcaag
     1321 actttgtgta ccgaacttgg tctgacaatc tcctgcgttt ccgctacaaa caactttacc
     1381 gtagccagcc aagaagaact ggatcgtgag atcgagcacg tcaagggctt tgttgatgtt
     1441 gcttctatcc ttggcgcgcc actggtaagg atttttgcag gagtttccag ccagctcact
     1501 gacgatgaag tctgggctaa tgtggttgct gctatcagag agtgtgccga gtatgcactt
     1561 gagcacggaa tcatgctcgc tctcgagaac cacggcggtt tcatggacga tcagatcctc
     1621 cgcatcatcg aagacgtaga tatggaaaac ttcaaagtta caatggacct tggtaatttt
     1681 ggcacagatc ccaacattat atacaaggca atggagaacg ttgcgccgca tactatattt
     1741 gtacacgcca aatgtatgga gtttacagag gactacctcg agaagactct cgactataag
     1801 agaatcatga agatcctgga cgatgcaggc ttcaacggct ttttgtccat cgagtttgag
     1861 ggaccgggag aggaaaaaga agaggtagga aaagctgttg ctatgctccg caacctgacc
     1921 ggtgctattc acaaggagca ggtcgacaag ctggttaagt aatggctcag cccaaagtgg
     1981 ctgactaaag aaaggggtga accggatggg caaattctac tacggtgggc aagcagttat
     2041 ggaaggcgtc atgatgcgcg gacctaaaac catcagcatc gccgtacgca aggaagacaa
     2101 atcgcttata gttcacaaag aagatgtcac gtcaatcggc gaaaggtttc ccattctcaa
     2161 aaaaccattt atcagaggca cggtttcttt ggtggagtcc ctggtcatcg ggatgaaagc
     2221 cctgaccttc tcggccaatc agttcgcttc agaagatgag caggaagagc tctccccaaa
     2281 agaaatggtc ctcacactag gtctttcctt cggtcttgcc atactacttt ttgttgtcct
     2341 gccggccttt cttactcggc tgattgggca gtatatgcag agtactctgc tgatcaactt
     2401 cgtagaaggg ctcattaagc tggtgctttt ccttctatat attgtcggga tttcgttttt
     2461 gtccgacatt aaaaggtttt tccagtatca cggtgcagag cacaaagtga tcaacgctta
     2521 cgaggcaggg gaagaactta ccgtagaaaa cgtcaggaag cattcccgct ttcacatgag
     2581 gtgcggcact aactttatca ccatcgttct aatcctcaat atcttcatct tcaccctggt
     2641 taccggggga gcacgtctgg cgtttttccc aagggtagga gcgcatctac tcctgattcc
     2701 cgttgttgcg ggagtctcct atgaggttct gagattcctc ggcaaggaag atgcgccgaa
     2761 ttgggcaaaa gcccttgcac gtccgggcct tgcaacacaa atcataacca ccagggagcc
     2821 tgatgatagc cagattgaaa ctgccatact tgccctaaaa gaagtaatgg gagcagatca
     2881 atcctcaaat gaaaaggcta tagtatagat tacgacgaaa ctcatctaac cgcggccttc
     2941 tggctgcggt taatttccga atggagggag tgagaccatg ctagataagt tggaagcgat
     3001 tgagcaactc tacaacgaac taacggaaaa aatggccgac cccaaactgg cccaggacag
     3061 gcaggagtac caaaaggtag gcaaaaggcg ctcggagctc gaggagatag tcgaagctta
     3121 ccaccgctac aaagacatcc aaaaccagat cgagggcgcc aaagaacttc tgcgggaaac
     3181 caccgatccg gagttgagag agctggccga agtagaactt gaagaactag aggagacttt
     3241 ggcaagcact gaaagcgaac tgaagatgct cctgctcccg aaggatccca acgatgagaa
     3301 aaacgttatt gtggagatcc gcgcaggtac aggcggtgac gaagcagctc tgtttgccgg
     3361 aaacctctac cgcatgtaca gccattacgc cgaaggccgc cgctggaagc tagaggtttt
     3421 aagcggcaac gctaccgagc ttggcggatt caaagagatc atctttatga tcgaaggtaa
     3481 aggtgcctac agccgcttga agtatgaaag cggcgtgcac cgagtgcagc gagttccgga
     3541 aactgagtcg ggaggacgca tccacacttc aactgccaca gtagcggtgc ttcctgaagc
     3601 agaagaagtg gatgtctata ttgatcctaa cgacatcaaa atcgacgtgt accgctcttc
     3661 aggtccggga ggacagtcgg taaacaccac agactctgct gtgcgcatta cgcacattcc
     3721 cactgggttg gttgtttctt gtcaggatga aaaatcccag cataaaaaca gagacaaggc
     3781 tatgaagatt ctcagagcca ggctcaaaga gatggctgag atggaagccc gttccaaaga
     3841 agctcaggag aggaagagcc agataggtac aggagatcgc agcgaaagaa tccgtaccta
     3901 caacttccca cagggacgcg tcaccgacca cagaatcaac ttgactctat accgaattga
     3961 agaaatacta aacggcgact tagatgaaat catcgatgca ctatctctag ctgatcaaga
     4021 agagcggcta aaggagatgg agtaaaatgc ctcaactgct ggagattctt aactcagcgg
     4081 gagagtattt gcagaaaaag ggcagtcctt caccgaggct ggacgcagag gtgctccttg
     4141 cggacatctt gggtatgcag cggatcgatc tctatgcaaa gttcgacagg cctttggacc
     4201 aaaaggaact ggctctctac cgcaaggcca ttgccaggcg ggcaaacatg gaacctgtgg
     4261 cctacattat cggccgcaag gagttctact ctcgagagtt tacggtaact cccgatgttc
     4321 tcataccgag acctgacacc gaggtcatcg ttgagaaagt catcagctac gccaagtctt
     4381 ttggcatcga atctccggag attctggacc tgtgtaccgg gtccggggca atcgccatca
     4441 ccctcaagct ggagattccg tcaagccaag ttactgcaac ggagatcagc gagaaggctc
     4501 tggagatcgc ctggaaaaac gcggcaaaac tcggtgcaga cgtaagtttt gttcagggag
     4561 acctgtatgc tggtatagac aagcagtttg acatta
//
LOCUS       DAOSDA010000087         4594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_11866_length_4594_cov_2.985680, whole genome shotgun sequence.
ACCESSION   DAOSDA010000087 DAOSDA010000000
VERSION     DAOSDA010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11866_length_4594_cov_2.985680"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PLW82_02975"
     CDS             complement(<1..166)
                     /locus_tag="PLW82_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOJ46268.1"
                     /translation="MVGKPVLLTQEGLKRLEEELDYLKRIKRREVAQRFRDALAFGDI
                     SENSEIVELFF"
     gene            complement(571..1662)
                     /locus_tag="PLW82_02980"
     CDS             complement(571..1662)
                     /locus_tag="PLW82_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOJ46269.1"
                     /translation="MERFGFVLHPISYSDISRKFGRMTKVLPESLVLSVMKNLPAQKV
                     SHITGIKSQTGVEAEGWFTACPLTTEQMVSLPEDFVIKKIIAAVNLAKEQGAKIVGLG
                     AYTSVVGDAGITIARNVDVPVTTGNTYTVATALLGLEWAAERMGTSLEASRVGVVGAY
                     GSIGKACARALAGKVGELCLIGRKKSELEKLKAELDAKGNISISTDSKTTLPTLDALV
                     TVTSAVDTVIEPEDLKIGAIVCDVARPRDVSKKVAEVRDDVLVFEGGAVEVPGENADF
                     HFNFGFPPKTSYACMAETMILALEKCYECYTLGRDLDVEKVYEMMRLAEKHGFKLAGL
                     RSFERAVTDEEIYRIRENIAKKKLALVKV"
     gene            complement(1675..2577)
                     /locus_tag="PLW82_02985"
     CDS             complement(1675..2577)
                     /locus_tag="PLW82_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HOJ46270.1"
                     /translation="MKQIISVSIGSSKRNHEVQIDLMGEEFLIKRIGTDGDIDKAIEI
                     IKDLDGKVDAIGLGGIDFYLFSKGRRYIIHDSKRFLNATKTTPILDGSGLKNTLERKI
                     IRDLQESGSVDFRGKKVMLVCGLDRFGMGEEIAKLTDQMVIGDLMFALGIGIPLRSLN
                     ALDKVARVFAPLAIRLPFSVLYPTGKSQEEIVEKYGKYYNWADIIAGDFLMIKKHMPK
                     DLTGKIIITNTTTKDDMEELKRRGVEVLITTTPILNGRSFGTNVMESMLVAYAGKKGE
                     LSEDEYFELLDRLNFQPNIVWLQK"
     gene            complement(2574..3101)
                     /locus_tag="PLW82_02990"
     CDS             complement(2574..3101)
                     /locus_tag="PLW82_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOJ46271.1"
                     /translation="MGDKVLSRDKIIAKIDEILKLRQNGMSQIDVAKFLNLDRPFVSR
                     LESLGEVAKGGKIALVGFPIGNKEELEQMALEEGVEFVYVLTDKERWSLVSNTDGAML
                     VNDLLELLQHLKNFDTIILLASNLRSQYAESIIGKQVISIEIGKSPIKDDVEVDIEKV
                     RQIIREIRQKEGEKQ"
     gene            complement(3239..4207)
                     /gene="dusB"
                     /locus_tag="PLW82_02995"
     CDS             complement(3239..4207)
                     /gene="dusB"
                     /locus_tag="PLW82_02995"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOJ46272.1"
                     /translation="MRIGNVQLENSLVLAPMAGVTGKAYRIICKEQGAGLVVTEMISA
                     KALTYNDKNTKKLLEIDPKERPTSIQLFGSEPETMAEAARICAAEAAPDIIDINMGCP
                     APKIVKNEDGSALLKEPKKAVEVARRVVEAVDIPVTCKIRLGWDESSLVHKELAKGLE
                     AVGVSAIVVHGRVRQQFYSGTADWQAIKEVVEAVSIPVIGNGDIWEAEDALKMERETG
                     AAGVAIGRGALGNPWIFRDILALKRQETVEPVTLHEKLAMAIRHAELLMEYRSERNAM
                     LEMRKHMAWYLKRERNAKVYRQEINSISRFTDLERLVQKILDEANS"
     gene            complement(4256..4453)
                     /gene="rpmE"
                     /locus_tag="PLW82_03000"
     CDS             complement(4256..4453)
                     /gene="rpmE"
                     /locus_tag="PLW82_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOJ46273.1"
                     /translation="MRENIHPKYFNAKVTCACGNTFETGSTKPELRVDICSKCHPFYT
                     GKQKIIDTGGRVERFKKKYNL"
BASE COUNT         1049 a         1205 c         1009 g         1331 t
ORIGIN      
        1 cgaagaagag ctcgactatc tcagagttct cacttatatc tccaaaagca agcgcatctc
       61 tgaagcgctg tgctacttct cgccttttaa ttctcttgag atagtcgagc tcttcttcga
      121 ggcgtttgag accttcttga gttaatagta ctggcttacc taccattaaa actcccttct
      181 ttctctggga cgcaatagtg tttctgacac gaaaaaaaca ctagattgtt gcaatctagt
      241 atcacagcag atctcgagat ggattcgtcc ctagctattt agtatattat ggcctaaatg
      301 atagttttat tcagactgca gcgtgagttt aaaataaagt tgggcagaag tagcatctgc
      361 cgttgttgcc tatcattata atgagtccac cgatcattgt caagaattat caaaaaacgc
      421 tgtggtcacc ctggcagaat ctggctatct actgatcgga ctaacgcaag tactgcacca
      481 ccaacacctt ctagttaatt agaccttagg tttgggaatc ctgcaagatg cagcaaattt
      541 aaaagaggcg caaagtcgcg cctctaaaag ttataccttg accaaagcca gtttcttttt
      601 ggcaatgttc tccctgatgc gatagatctc ttcatctgtt acagccctct caaaagagcg
      661 aagtcctgcc agcttgaaac cgtgcttctc ggccagccgc atcatttcgt agaccttttc
      721 cacatccagg tcgcggccta acgtgtagca ctcgtagcac ttctccagcg ccaaaatcat
      781 cgtttcggcc atacaggcgt acgaggtttt cggtggaaag ccgaagttga agtggaagtc
      841 ggcgttctcg cccggcactt ccacagctcc accttcaaag accaaaacgt cgtctctgac
      901 ttcagcgact ttcttagaaa cgtcccgcgg tcttgccaca tcgcagacga tggccccgat
      961 cttcaggtcc tcaggttcaa tcaccgtatc gaccgcactg gttaccgtca ctaacgcatc
     1021 taaagtggga agggttgtct tggagtcggt acttatagaa atgttgccct ttgcatcaag
     1081 ctctgctttg agcttttcca gttctgactt cttcctgccg ataaggcaga gctctcctac
     1141 cttcccggca agagcgcgag cacaggcctt tccgatagac ccataagcac ctaccacgcc
     1201 cactcgactt gcttcgagac ttgttcccat tctttctgca gcccattcta accccagtag
     1261 agcagttgcc acagtgtagg tatttcctgt tgttacgggc acgtcgacat ttcttgcaat
     1321 ggtaattcct gcatcgccca caactgaagt gtaagctcca agtcccacga tctttgcacc
     1381 ctgctctttg gcaaggttaa cggctgcgat gatctttttg atcacaaagt cctctggcaa
     1441 actcaccatc tgctcggtgg tcaaaggaca ggcggtgaac cagccttcgg cttcaactcc
     1501 ggtttgggac ttgattcccg ttatatgcga aaccttctgt gcggggaggt ttttcatcac
     1561 tgacagaacc agcgactctg gcaggacttt tgtcattcta ccaaatttac ggctaatatc
     1621 actgtaggat attgggtgca gtacaaatcc aaagcgttcc aaaatactcc acctctattt
     1681 ctgcaaccat acaatgtttg gttggaagtt taaccggtct aaaagttcga agtactcatc
     1741 ttcagaaagt tctccctttt tccccgcata agcgacaagc atcgattcca tcacgttggt
     1801 gccgaaggat cttccgttca gtatgggagt tgtggtgatt agaacctcaa ctccacggcg
     1861 cttcagttcc tccatgtcgt ctttggttgt agtgttggtg atgatgatct tgccggtaag
     1921 gtctttcggc atgtgctttt tgatcatcag gaaatcgcct gcaatgatgt cggcccaatt
     1981 gtagtacttc ccatacttct ccacgatctc ttcctggctc ttccctgtgg gatagagcac
     2041 ggaaaatggc agcctgatcg caagcggtgc aaagactctc gcaaccttat ccagggcgtt
     2101 cagagagcgg agggggattc ctatgcccag ggcaaacatc agatcgccga ttaccatctg
     2161 atctgtgagt ttagcgatct cctcacccat gccgaagcgg tccaggccgc agaccaacat
     2221 gaccttcttc cctctaaagt ctacgcttcc gctttcctga agatccctga tgatctttct
     2281 ctccaaagtg tttttcaggc cactcccgtc taaaatcggg gtggtttttg tggcattgag
     2341 gaatctcttc gagtcgtgaa tgatgtatcg tctacctttg gagaataggt agaagtctat
     2401 accgccgagg ccgatggcat cgactttacc atctagatct ttgatgatct cgatagcttt
     2461 atcgatgtcg ccgtctgtgc cgattctctt gatgagaaac tcttcaccca ttaagtcgat
     2521 ctggacttca tggtttctct ttgaggaacc aatactaaca ctgatgatct gtttcattgt
     2581 ttttcccctt ctttttggcg gatttccctg atgatctgcc tcaccttctc gatgtccact
     2641 tccacatcgt ctttgatcgg cgacttgccg atttcgatgg atatgacctg cttgccgatg
     2701 atgctctctg catactgact gcggagattt gaggcaagga gaatgatggt atcgaagttt
     2761 ttgagatgct gtaaaagctc gagaagatca ttaactaaca ttgctccatc ggtgttggaa
     2821 accagactcc agcgttcttt gtcggtgagt acgtagacaa actctacccc ttcttcaaga
     2881 gccatctgtt ccaattcttc tttattgccg atgggaaaac ccactagggc aatcttgccg
     2941 ccttttgcca cctcgcctaa agactccagt ctcgatacaa aaggccggtc cagattgaga
     3001 aacttggcaa cgtcaatctg cgacataccg ttttgacgta gcttcaagat ctcatcgatc
     3061 ttggcgatga ttttgtcgcg acttagaact ttgtctccca cccgaatgag cttgacagag
     3121 gtgcttatac gccatcaccc cttatgatgt acttaaatcg tgaggtgtgt gcacattttt
     3181 gtgtacagca ttattttacc aacgggggct ttaaaagtca acgaactcct aggttatcct
     3241 aggagttcgc ttcatccagt attttttgca ctaaccgctc aagatcggta aagcggctga
     3301 tgctgtttat ctcctggcgg tagactttag cgtttctttc tcttttgaga taccaggcca
     3361 tgtgcttgcg catctccagc atggcgtttc gctcgcttct gtactccatg agaagctcgg
     3421 catgcctgat cgccatcgcc agcttttcgt gaagtgtcac tggttccacc gtttcttgtc
     3481 gcttgagtgc taggatatct cggaagatcc aaggattacc tagagcgccg cgcccgatag
     3541 caactccagc tgcaccggtc tctctttcca tctttagggc gtcttccgct tcccagatat
     3601 cgccgttacc gatcacggga atggagaccg cctccaccac ttctttgatg gcctgccagt
     3661 cggcggtgcc actgtaaaac tgctgtctta ccctgccgtg gactacgata gcgctgacgc
     3721 caacagcttc gaggcctttg gcaagttctt tgtgcaccaa gctgctttca tcccaaccca
     3781 agcggatctt gcaggtcacc gggatgtcca ccgcttccac aactctcctt gctacctcca
     3841 cagccttttt aggctctttc agaagtgccg aaccgtcttc atttttcacg attttcggcg
     3901 cagggcagcc catgttgata tcgatgatat caggcgctgc ttcagccgca cagatccgag
     3961 cggcctctgc catagtctcc ggctctgaac cgaaaagctg aatgcttgtg ggccgttctt
     4021 taggatctat ctccaaaagc ttttttgtgt ttttatcgtt gtaggtgaga gctttggcac
     4081 tgatcatttc ggtgaccact aagcccgctc cctgctcctt acagataatg cggtaggctt
     4141 tccctgtcac accggccatg ggagcaagga ctaaagagtt ttccagttgc acgtttccga
     4201 ttctaatact cttcaccccc gaaaaaaaga aggcaggctc agcctgccat ggttcttata
     4261 ggttgtactt tttcttaaag cgttctacac gtccaccagt atcgataatc ttctgtttgc
     4321 cagtgtagaa aggatggcac ttcgagcata tatcgacacg gagctcgggt tttgtagatc
     4381 ctgtttcaaa agtatttccg caggcacagg tcaccttggc gttaaaatac tttggatgta
     4441 tgttttctct caacggattc acctcatttt ccccatataa aactcgcgtt ttctattgta
     4501 gcataggaca cccgaataat caagaaagag acggacaatc atgtggttat cttgtgatag
     4561 tgtcagggct gagatgtttt gggaatatgt catc
//
LOCUS       DAOSDA010000088         4534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12052_length_4534_cov_3.632284, whole genome shotgun sequence.
ACCESSION   DAOSDA010000088 DAOSDA010000000
VERSION     DAOSDA010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12052_length_4534_cov_3.632284"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLW82_03005"
     CDS             <1..199
                     /locus_tag="PLW82_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008563684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HOJ46274.1"
                     /translation="KFKNKKGAAFGSYGWSGEGVKLITGLLEGAGFEVVNEGFRAMWQ
                     PDEEALNAAKEFGKNFAESVK"
     gene            332..997
                     /locus_tag="PLW82_03010"
     CDS             332..997
                     /locus_tag="PLW82_03010"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOJ46275.1"
                     /translation="MKLFVDPPLPGKTNMEIDLELVEKAAETGSAYVRLYSWSRPTIS
                     LGYFQKPEKVLDFSALEKHGIDWVRRPTGGRAVFHFKEITYSVSLPEDYPGLPSSVTE
                     AYRFISEPIYRAFLQIGLPVKWARQEENVSEACFLAPARHEIILQGKKVVGSAQRRKA
                     RSVLQHGSILLGAQPEVFADCLLGVDKEVFLEQMRTRTMVIPVREEELRKSLIYQFRR
                     FFS"
     gene            1180..1881
                     /locus_tag="PLW82_03015"
     CDS             1180..1881
                     /locus_tag="PLW82_03015"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ46276.1"
                     /translation="MTWKQKIGILFLSVVLFAVSSAAQDIYLAPRAKRVVVDGKLNEW
                     EGVPAITLNSNAYIVIANRSWKGPADLSGKIYVMWDEEYLYVACEIEDDVVIQEKEGT
                     YLFQGDCIELYVRLDYEKNALLNYYTLTDFQFGFTPGTNAGAPDYHIWNNSTVLEDIL
                     FEAAKTPTGYIMEVAIPVWELGIFLDAGMEIGFDVAIDDVDSPGAQDTEQQLCWSMSD
                     MGWQDPTVFQLVILD"
     gene            2017..2322
                     /locus_tag="PLW82_03020"
     CDS             2017..2322
                     /locus_tag="PLW82_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46277.1"
                     /translation="MDQQPNVPKWLIRLQIIFGIFLIVASIFLYYTIVPKGLDQKVVL
                     ILLGFGLLNLFFGRFLFLLPQKQKERAKRLKEEKEREMRQAAKQKAKENKAKGKKKR"
     gene            2319..2531
                     /locus_tag="PLW82_03025"
     CDS             2319..2531
                     /locus_tag="PLW82_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOJ46278.1"
                     /translation="MSPGKFYVGDVVRMRKPHPCGGNTWEVMRVGMDFRIRCLTCDRV
                     VLIPRVKFEKGLKEVIQRGPIPENEE"
     gene            2546..3622
                     /gene="ychF"
                     /locus_tag="PLW82_03030"
     CDS             2546..3622
                     /gene="ychF"
                     /locus_tag="PLW82_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOJ46279.1"
                     /translation="MDIGIVGLPNVGKSTLFNALTKAGAEVANYPFCTINPQVGVVPV
                     PDRRLAKLGEILQPEKLTQTTIQFIDIAGLVKGASKGEGLGNQFLAHIREVDAICHVV
                     RFFESPNVVHVDGKLNPLADVEIIETELALKDLETVEKRKEKTSKNLKTGKPEYKEEM
                     AELEELAGLLAAGRKPPRTPLVMELQLLTAKPCIYIANMDEEQIQNPESVPMYKEFVE
                     YARGNGNEVIPVSADLEMGLLELDEEERAEYFAALGITYQSGLERVVARSYALLDLIT
                     FYTVKGPETRAWTVKRGTKAPQAAGKIHSDMEEGFIKAEVVSFEKLVEIGSFALARDK
                     GEVRVEGKDYEIQDGDVVLFRFKN"
     gene            3726..4481
                     /locus_tag="PLW82_03035"
     CDS             3726..4481
                     /locus_tag="PLW82_03035"
                     /inference="COORDINATES: protein motif:HMM:NF022354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection system antitoxin AbiGi family
                     protein"
                     /protein_id="HOJ46280.1"
                     /translation="MSRQRYFSKVYWHFTGSPEDTDWSLIKKPADLRAQGKRPKRLSK
                     ATDILCKILESKTLLATCREKLFRDLVTDEFCCVCDIPIKDLTTHAPYYGKVAIGFSA
                     EAVYRAGFNPVLYLSDDNIILGVASDPIGEADDLIGEADEPFYTLEPSENFAESASAV
                     ESLGYLFYGSTKAFFGENYHYLKFTTFDVNDDHSFYREREWRLLRDFRFAVEDVEAIL
                     APKSQLKKIRDTLAKNGYPDSISLLSWEFLERA"
BASE COUNT         1290 a          974 c         1195 g         1075 t
ORIGIN      
        1 gaagttcaaa aacaagaaag gtgcagcctt tggcagttac ggttggagcg gagaaggcgt
       61 taaactgata accggtctct tggagggcgc tggatttgaa gtggtaaacg aaggctttag
      121 ggcaatgtgg cagcccgatg aagaggcttt aaacgctgca aaagagttcg gaaagaactt
      181 tgccgagagc gtaaaataaa actccatact agtggtacag caagaaaccg cctttttcgg
      241 gcggtttttt gctgggaact acttttgaaa aaaggagttg cttcgggaag gcagcgaagt
      301 aaataagaga gcacaatcga ggtgatttgc attgaagctt ttcgtcgatc caccgcttcc
      361 cgggaaaact aatatggaga tagacctgga acttgtggaa aaggccgcag agaccggatc
      421 tgcctacgta aggctttaca gctggagcag gcctaccatc agcctgggct acttccaaaa
      481 gccagaaaag gtgctggact tctctgccct ggagaaacac ggcatcgatt gggtgagaag
      541 acctacaggg gggagagcgg tttttcactt caaagagatt acctactcgg tctcgctccc
      601 tgaagactat cccggtcttc ctagctcggt aacggaagcc tatcgcttca tttccgagcc
      661 aatctacagg gcatttctac agataggact gccggtaaag tgggcaaggc aagaagaaaa
      721 cgtctcagag gcatgctttt tagctcctgc ccggcacgaa atcatcctgc aggggaaaaa
      781 ggtggtagga agtgcccaaa gaagaaaggc cagaagcgtt ctgcagcacg gatcaattct
      841 acttggtgcc cagcctgaag tcttcgcaga ctgtctcttg ggcgtagata aagaagtttt
      901 tctagagcaa atgcgcacca ggacaatggt gatacctgta agagaagaag aactgagaaa
      961 gagtttaatc taccagttta gaaggttttt ttcctgaaat agaagcttac tttttcgttg
     1021 aatctctaca agacgactga ccgctacgcg cggagcggtc agtttttgct tcaaaaggct
     1081 cgttagggct gcgtagttta gcgatgatgg ttggaataag aaggataaaa cccgtagatg
     1141 accaaatata ctaatacaat gaataagggg gttatggctt tgacttggaa gcaaaaaatc
     1201 gggattttat ttctctcagt agtgcttttc gccgtttcgt ctgcggcaca ggacatttac
     1261 ttggctccca gggcaaaaag ggtggttgtg gacgggaaac ttaacgagtg ggaaggggtt
     1321 cctgcaataa ccctcaacag caacgcatac atcgtcattg ccaacagatc ttggaagggt
     1381 ccggcagacc tgtctggtaa gatttatgtg atgtgggatg aagagtacct ctacgtcgcc
     1441 tgcgagatcg aagatgacgt tgtcattcaa gaaaaggaag gcacatatct cttccagggc
     1501 gattgtattg agctttacgt aaggttggat tacgagaaaa atgcccttct caactactac
     1561 acattaaccg acttccagtt tggctttact ccgggcacca acgctggagc accggactac
     1621 catatctgga acaactcgac ggttttggag gacattcttt ttgaagcagc aaagacgcct
     1681 acaggataca tcatggaagt agccattcct gtttgggaat tgggcatctt cctcgatgcc
     1741 ggtatggaga tcggcttcga cgtggctatc gatgatgtgg attccccagg cgctcaggac
     1801 actgaacagc agctgtgctg gagcatgagc gacatgggct ggcaggatcc aacagtattc
     1861 caacttgtaa tcctcgactg atggaaaagc tcctgtttat caggagcttt ttagtcatga
     1921 ggaaaagccc ccgaaaagga ttgagcactg caggggaaaa tagatagaat aagaagaaaa
     1981 gctattagag aaagacttag agagaggcgt tgaatcatgg atcagcaacc taatgttcca
     2041 aagtggttaa tcagactaca gatcattttt ggcatatttc taattgtggc atcgattttt
     2101 ctctactaca ccattgttcc taagggctta gaccagaaag tcgttttgat tctgcttggc
     2161 tttggtcttc tcaacctctt ctttggacgc tttctctttc ttctgcccca gaaacaaaaa
     2221 gaacgggcaa aaaggctgaa agaagaaaaa gaaagagaga tgagacaggc agcaaagcaa
     2281 aaggccaaag aaaacaaggc taaggggaag aagaagcgat gagtcctggc aagttttacg
     2341 taggtgatgt tgtgagaatg agaaaacccc atccctgtgg aggcaacact tgggaagtta
     2401 tgcgtgttgg catggatttt cgcattcgct gcctaacctg cgatagggtt gtcctaattc
     2461 ccagggtaaa gtttgaaaaa ggtctgaaag aggtcattca acgcggtcct ataccggaga
     2521 atgaggagta agggaggggt tctctttgga tataggtatt gtgggacttc ccaacgtggg
     2581 caagtccact ctatttaacg cattaacaaa ggcaggtgca gaggttgcga actacccctt
     2641 ctgcaccatt aatcctcaag taggtgtggt tccggtgccc gacaggaggc ttgccaagct
     2701 gggagagatc ctgcagccag aaaaactcac tcaaacaacc attcagttta tcgatatcgc
     2761 aggcctggtt aaaggagcaa gcaaaggcga aggcctgggg aaccagttct tggcgcacat
     2821 cagggaagta gatgccatct gccacgtggt gcgctttttt gaaagcccca acgtggtaca
     2881 cgtggatgga aagctgaacc ccctcgcaga tgtggagatc atcgaaacag agcttgccct
     2941 aaaggatcta gagaccgttg aaaagcgtaa ggaaaaaacc agcaagaact tgaaaaccgg
     3001 taaacccgag tacaaagaag agatggcaga actagaagag ctcgcaggtc ttctagcagc
     3061 agggagaaag cctcctagga ctcctctggt catggagcta cagctgctta cagccaaacc
     3121 ctgcatctac attgccaaca tggatgagga gcagattcaa aaccccgaat ctgtccctat
     3181 gtataaagag tttgtggagt atgctagggg aaacggcaat gaggttatcc ccgtttcggc
     3241 agatctggag atgggccttt tggaactgga tgaagaagag cgggcagagt attttgctgc
     3301 tttaggcata acctaccaga gcggcctgga aagagtggtt gccaggagtt atgccctctt
     3361 ggatctgatt accttctaca cagtgaaggg tcccgagacc agggcttgga ccgtgaaaag
     3421 aggcactaaa gctcctcagg cagcagggaa aatccactcg gatatggagg aaggctttat
     3481 caaagccgaa gtggttagct ttgagaagct tgtagagatc ggttcatttg ccctcgctag
     3541 agacaaaggc gaagttcgcg ttgagggtaa agactacgag attcaagacg gcgatgtagt
     3601 gctgttcagg ttcaaaaact gatagagaag cacaacttaa aaaccgtttc caactgtgag
     3661 cgttccaggt ttcttgtgtt tcagcaaaaa tgcttcgatc ttgttgtgac tgagaggtta
     3721 gtggtatgtc aagacaacgc tacttttcga aagtctactg gcattttacc ggaagcccgg
     3781 aagacactga ttggtctcta attaaaaagc ccgcagatct aagggcacag gggaaaagac
     3841 cgaagcgtct gtcaaaggct acagacattc tttgcaagat acttgagtca aaaacgctgc
     3901 ttgcaacctg cagggaaaaa ctcttcaggg acttggtcac agacgagttc tgctgcgtct
     3961 gcgacattcc catcaaggac cttacgacgc atgccccgta ctacggcaaa gtagccattg
     4021 gctttagtgc cgaggcggtt tacagggcgg ggttcaaccc agtgctctat ctctccgatg
     4081 acaacatcat tttaggcgta gctagcgatc ctatcggaga agcagacgac ctcattgggg
     4141 aagcagatga gcctttctac acgctggagc caagcgaaaa ctttgccgaa agcgcatctg
     4201 cagtggagtc cttaggctac ttgttttacg gctctacaaa agcgtttttc ggagagaact
     4261 accactacct gaagttcact acctttgatg tcaacgacga tcacagcttc tatagagaaa
     4321 gagagtggcg actgctcaga gatttccgct ttgcagttga agacgttgag gccattctcg
     4381 ctcctaaaag ccagcttaaa aagatccggg atacgcttgc aaaaaacggc tacccagatt
     4441 ccatttctct tctaagctgg gagtttttgg aacgagctta gtccaaagcg gcagagaacg
     4501 atttttgtga aactcatttt cttacacgtc tctt
//
LOCUS       DAOSDA010000089         4519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12107_length_4519_cov_2.888665, whole genome shotgun sequence.
ACCESSION   DAOSDA010000089 DAOSDA010000000
VERSION     DAOSDA010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12107_length_4519_cov_2.888665"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLW82_03040"
     CDS             <1..470
                     /locus_tag="PLW82_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46281.1"
                     /translation="SYSYYVCPKRRGKLCLLKPLPTEALDHLVWVEFEKRFAHAAPKF
                     REYEKKLENDRRERLLESKNKELGNIKHREEKLLDLLLSGVINPEVYREEKKSVERSK
                     KVLLEDIERLGQEVPDAPYSTKVPPNLRKKALEIFTEKILVTATGEIRVYALI"
     gene            568..1107
                     /locus_tag="PLW82_03045"
     CDS             568..1107
                     /locus_tag="PLW82_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOJ46282.1"
                     /translation="MSRLIPNRWYDKIWHIPVKDLADIGIKGVIVDLDNTLVPWKKDP
                     SHASLETKEWLKKAQDAGMDICIVSNGGSSRVQSFAAELGIKAVAGVPKPRRTPFLRA
                     MSLMGTSPKETAVIGDQLFTDILGGNRAGCYTILVTPHDEKEFFYTRLVRILERKVKE
                     RLQKKGAFPERKVNGDENL"
     gene            1094..2167
                     /locus_tag="PLW82_03050"
     CDS             1094..2167
                     /locus_tag="PLW82_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOJ46283.1"
                     /translation="MKTCKGCGIDLQTNDPNLPGYVPENVYEKAEVCQRCFRLTHYAD
                     YTASPLSEEDFMDAATKALDGADTVFQVFDIIDFESSLNPTFEKLLFRKNTVAVVTKY
                     DLLPEIVSEKDVEEWVKKRVPYADAVAVVSGKKGWGIDKLKDMRGRVTAVVGTTNAGK
                     STLIQRLVQGAKPTVSPSLGTTLGNLTFEDEKGVIVDTPGLKPNGRLLEMLCPVCSAS
                     LVPNKRLNSKLFQLKPGQGLALGNLFYLSLKSENPVVVMAYVPESVSISRVNRDRAES
                     FLEEAGAPDAIPCAECKPKIVFEKRDLRVGDEDLVVPGLGWVSFRHTPASFEVICPKG
                     LGLPRRSPLVRRGKHTTTGKRIR"
     gene            2358..3761
                     /locus_tag="PLW82_03055"
     CDS             2358..3761
                     /locus_tag="PLW82_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOJ46284.1"
                     /translation="MEIFEQITKDDAKEILEVALSGGGDFADLYVESRTATSILCEED
                     RIENISTGIDCGAGIRVVDEDRVLYIQTNDLTIEGLKKAAEEAALAVGKSRKVNPKEL
                     VESDLVESTVEIDPSAVPISEKTEAVLAANNACRACSDKIVQVTVGYVDGKREILIAN
                     SEGIFTKESRVSTRFSVNAVARDGDVVQTGYESRGGVQGFEVMKGAEELAQIAGDRAV
                     KMLSAKPAPAGKMTVVMSSSAGGTMVHEACGHGLEGDLVYRGMSVYAGKLGEVVASEK
                     VTVIDDATMEGKYGYYGYDDEGTKGQKTVLIENGVLKTYMTDLRYAKKLGLEPTGNGR
                     RQSYAHRPVPRMSNTYIAAGTDDPDEIIRSTEKGLFVTKMGGGQVNTTNGEFVFDVAE
                     GYLIEDGKIGPLVRGATLIGNGPQVLRDIEMVGSDLGFAIGVCGKEGQGVPVSDAQPT
                     IKIKSMTIGGLVEEEDK"
     gene            3758..>4519
                     /locus_tag="PLW82_03060"
     CDS             3758..>4519
                     /locus_tag="PLW82_03060"
                     /inference="COORDINATES: protein motif:HMM:NF040244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HOJ46285.1"
                     /translation="MNLKEIKEIAMRAPQLGAQAEIFVMRSQSLSLQVSDQKLENMDR
                     KNTQGVGIRALVDGRMGFAFSTVLSKESVEYTLRQAIENAKVTAPDQFNILPGKNVAI
                     GEKETLADVSFEEKLENLMLAERKAKDYDPRIVKVQRVGYSDSLAEIMIASSEGVEAE
                     YTNSYYSAYVSSQAEEDGNVEMGTSYDVVRDFAKINFRNLGEESADKAVSMLGAKPIA
                     SEKLPVILDREVATSVLEIVADILSARAVIKKKSLF"
BASE COUNT         1303 a          994 c         1256 g          966 t
ORIGIN      
        1 agtcctacag ctactacgtc tgtccgaaaa gacggggaaa actctgctta ctcaaaccgc
       61 tgcctacaga ggctctggac catctggtgt gggtggagtt tgaaaagcgc tttgctcatg
      121 ctgctcccaa gttccgagag tacgaaaaaa agctggaaaa tgatcgcagg gagcgactgc
      181 tagagagtaa aaacaaagag cttggtaata ttaagcatag agaggaaaag ctactggatt
      241 tgttgctctc tggcgtaatt aacccggagg tatacaggga agagaaaaaa agtgtagaaa
      301 gaagtaaaaa ggttttgctc gaagatattg aaaggctagg gcaggaggtt ccagatgcac
      361 cgtattccac aaaggtacct ccaaacctgc gcaagaaagc tttggagatt tttaccgaaa
      421 aaattcttgt aacagcaacc ggcgagatcc gggtttacgc actgatctag gcatttgcgc
      481 tatctgaccc actcattgag aagagagcca tatgcaaaat ccacaaagca atgctcctgg
      541 acggttacaa gtgacgagga ggaagtaatg agtcgtttaa ttcctaatag atggtatgac
      601 aaaatatggc atattccagt gaaggacctg gcagatattg gcattaaagg cgttatagtg
      661 gacttggata acactttggt tccctggaaa aaggaccctt cgcacgcaag cctagagacg
      721 aaagagtggc tgaagaaggc ccaggatgct gggatggaca tctgcatcgt ctctaacgga
      781 ggaagcagca gggtgcagag ctttgctgct gaactcggta tcaaagctgt ggcaggggtc
      841 cccaagcccc ggcgtacgcc ttttctccgg gctatgtcgc tgatgggtac aagccccaag
      901 gaaaccgcgg tcatcggtga ccagctcttt accgatatcc tcggaggcaa cagggccgga
      961 tgctacacca tactggttac acctcacgat gaaaaagagt ttttttatac gagacttgtc
     1021 aggattttag agcgaaaggt gaaagaacgc cttcagaaaa aaggggcgtt tcctgagaga
     1081 aaggtgaacg gtgatgaaaa cctgtaaggg atgcggtatc gatctgcaga caaacgaccc
     1141 gaacctaccg ggctacgttc ccgagaacgt ttacgagaaa gctgaagtgt gtcagaggtg
     1201 ttttcggctg acccactacg ccgactatac ggcgtcacct ctttctgaag aagatttcat
     1261 ggatgcggca acgaaagcgc tggacggcgc cgacaccgtt ttccaggttt tcgatatcat
     1321 cgattttgag tccagcctga acccgacctt tgaaaaactt ttgttcagaa agaatactgt
     1381 agctgtggtg acaaaatacg atcttcttcc agagatagtc tctgaaaaag acgtggagga
     1441 atgggtcaaa aagcgggttc cctacgccga tgcagtggca gtagtaagcg gcaaaaaggg
     1501 ctggggcata gataaactta aggacatgag gggccgtgtc acggcagtgg tgggaacaac
     1561 caacgctgga aaatccacgc tgattcaaag gcttgtccaa ggggcaaagc ctacggtttc
     1621 accgtcacta ggaactactt tggggaacct tacctttgaa gatgaaaaag gcgtgattgt
     1681 ggacaccccg ggtttaaagc caaacggacg actgctggag atgctgtgcc ctgtctgcag
     1741 tgcgagtctt gtgccaaaca aaaggctgaa cagcaagctc ttccagctta aaccaggcca
     1801 ggggcttgcc ctgggtaatc tcttttatct gagtttaaag agtgaaaatc ccgtggtagt
     1861 catggcttac gtaccggagt ccgtctctat ttcccgcgtc aacagggata gagctgagag
     1921 ctttctggaa gaagcaggtg ccccagatgc cattccctgc gcagagtgca agccaaagat
     1981 agtctttgaa aaaagggatc tcagggtcgg cgatgaggat ctggtagttc cggggctcgg
     2041 ctgggtatct ttcaggcata cgcctgcatc ttttgaggtg atctgtccta aaggtttggg
     2101 actgccaaga agatcgcctc ttgtaagaag aggaaagcat acaaccaccg gtaagagaat
     2161 aaggtaaatt aacagatgat cataattact actaatgaat caaaaatgag ggagcgattg
     2221 tttctccggg ttttaaggca gagagatcta gcgttttcta ttctctccga acttaagcca
     2281 actgttgttt tcactggttc gtcctccttt cgcgcaattg gcaacctcat ttttaaagct
     2341 taggggggaa gagaaaaatg gagattttcg agcagatcac caaagacgat gcgaaagaga
     2401 tcctcgaggt ggcactttct ggaggaggag atttcgccga tctgtacgtt gaaagcagaa
     2461 cggcaactag cattttgtgt gaagaagatc gcatagagaa catctcaacc ggcattgatt
     2521 gcggtgccgg gatcagagtt gtcgacgaag atagagtact ttacatccag accaacgacc
     2581 taactatcga aggtcttaaa aaagctgccg aggaggcagc gctggcagta ggcaagagca
     2641 gaaaggtcaa ccccaaagaa ctcgttgaaa gcgaccttgt ggaaagcacg gtggaaatcg
     2701 atccaagcgc agtgccaatc agcgagaaga ccgaagcagt gcttgctgca aacaacgcgt
     2761 gcagggcttg ctctgacaaa atcgttcagg ttacagtggg ctacgtagac gggaagagag
     2821 agatcctgat cgctaactcc gaaggcattt ttaccaaaga gagcagagtt tcgacgaggt
     2881 tctctgtaaa cgcagtggcc agggacggtg atgtggtcca gaccggctat gaatctagag
     2941 gcggcgttca gggttttgaa gtcatgaagg gcgccgaaga actggcacag atcgcaggag
     3001 acagagcggt gaagatgctc agtgcaaaac cggctcctgc aggtaaaatg accgttgtca
     3061 tgagctcttc tgccggagga accatggttc acgaggcctg cggacacggc ttagaaggcg
     3121 atctggtgta ccgcggcatg agtgtctacg caggcaagct cggcgaggtt gtggcttcag
     3181 agaaagtcac ggttattgac gatgccacca tggagggtaa gtacggctac tacggttacg
     3241 acgacgaagg taccaaaggc cagaagaccg tgcttattga aaacggcgtt ctgaaaacct
     3301 acatgacgga cctccgctac gccaaaaagc tgggtctaga gcctacagga aatggccgga
     3361 ggcagtccta tgcccacaga cctgtgccta ggatgagcaa cacctacatt gcagcaggca
     3421 ccgacgaccc cgatgagatc atccggtcga cggaaaaggg actttttgtc acgaaaatgg
     3481 gcggaggcca ggtcaataca acaaacggag agtttgtttt tgatgtagcc gaaggctatc
     3541 tcatagaaga tggaaagatc ggacctttgg ttagaggagc aacgctgatc ggcaacggtc
     3601 cgcaggtgct cagagacatt gaaatggttg gaagcgactt gggttttgcc attggcgtct
     3661 gcggtaaaga aggtcaggga gttccggtat ccgacgcaca acctaccatc aaaatcaaaa
     3721 gcatgaccat tggcggcttg gttgaggagg aagacaaatg aacctaaaag agatcaaaga
     3781 gatagcgatg cgagctcctc aattaggtgc acaggcagag atcttcgtaa tgcgcagtca
     3841 atcgctatcc ctgcaagtgt ccgatcagaa gctcgaaaac atggatagga agaacaccca
     3901 gggggtaggc atcagagctc tcgttgacgg cagaatgggc tttgctttca gcacagttct
     3961 ctcgaaggaa agcgttgagt ataccctcag acaggccatt gagaatgcga aagtgacagc
     4021 accagaccag ttcaacatcc tgcccggcaa gaacgttgcc atcggggaga aggaaaccct
     4081 tgccgatgtc agctttgagg aaaaactgga aaacctcatg ctcgctgaaa gaaaggccaa
     4141 agactacgat ccgagaatcg ttaaggtaca gcgtgtaggc tacagcgatt ctttggcaga
     4201 gatcatgata gcaagctctg aaggcgtaga agcagagtac accaactcct actacagtgc
     4261 ttatgtttcc agccaagcag aggaagacgg caatgtggag atgggaacca gctacgatgt
     4321 ggtcagagac tttgccaaaa tcaacttccg caacctagga gaagagtcgg cagataaagc
     4381 cgtctccatg ctcggtgcaa agccgattgc ctctgaaaaa cttcctgtga ttttagacag
     4441 agaagtggca actagcgttt tggagatcgt ggccgatatc ctctcagcac gagcggtgat
     4501 caagaaaaag tccttgttt
//
LOCUS       DAOSDA010000090         4494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12178_length_4494_cov_3.750394, whole genome shotgun sequence.
ACCESSION   DAOSDA010000090 DAOSDA010000000
VERSION     DAOSDA010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12178_length_4494_cov_3.750394"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="PLW82_03065"
     CDS             complement(<1..132)
                     /locus_tag="PLW82_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ46286.1"
                     /translation="MTEGKISRSILLFALPIFLGDLFQQLYNVVDSLVVGNVLGKEAL
                     "
     gene            441..1655
                     /locus_tag="PLW82_03070"
     CDS             441..1655
                     /locus_tag="PLW82_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOJ46287.1"
                     /translation="MENLLDAILNMFKSDEIRKVVISKPSAKSLEYKKISAEKKGTYY
                     QIAKFTETQVFHENVGEGEVAERVYQLLASGFKQLNAWSDRFEHMILLSKKGQSTFKK
                     KALKEQTPVSIDAKHNREKNYLIKEGKPVEPLVDMGVLTPDGRVVKAMYDKYKQINRF
                     LEIIDDEVVAFQGKDIKIIDFGCGKSYLTFILYYYLTEIRKIPATIIGLDLKADVIEK
                     CNAAARKYGYTGLRFEVGDISQFEASLPVDMVITLHACDTATDFALYNAIKWDAEMIF
                     SVPCCQHELNKQIHTDNLSLFTRYGIIKERFSALATDAIRANLLEYAGYKTQLLEFVD
                     FAHTPKNILIRAVKRKSTSDSVREKALQEVEDVMREFNFNPTLYSLVVGGETLKGLVD
                     GNCCVRAPHEKD"
     gene            1907..3484
                     /locus_tag="PLW82_03075"
     CDS             1907..3484
                     /locus_tag="PLW82_03075"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ46288.1"
                     /translation="MDLRTVLLMLTVGSFLFGLLLVRFKFDRENPREVPYWIAAKFLQ
                     ATGSLLLYLGMGGPLMLLAKTVLILGCAHETWAIAIISGKKIKRWLHAVVDLAIIAVC
                     ALIALASETFRSSAIFSLQSIFYFLPFGFLVHKPDRKFSLQVPLGVLYLLTGLVFLVN
                     AAMLFFFPGYGLSGANVLLGMIPIISFCVFLASGFILLMLSKERSDLQMQQIQKSLKE
                     TEDRFRLIVETAIEGIMVFDEHYRITFANKNMASMLEYTVEEMLGRSYSSFFAEEDQD
                     IYREQEALRKSGRDSVYECGLLTKSGKKHWFLISARPLLGQSNRFEGSFAMLTDINER
                     KVMEMALAEANRQLIELSNRDGLTGISNRRRFDTFLESEFSRLKAENAHLSIIFLDVD
                     HFKAYNDLYGHLMGDECLKQIANVLANCVTRPDDLVARYGGEEFACVLPDTDLANAVL
                     KAEEIQEKVMELAIEHKGSKVSDLITVSFGVATFNSASGVTIEEMMKFADKQLYKAKS
                     LGRNRIEAAKWPGHSVA"
     gene            3709..4080
                     /locus_tag="PLW82_03080"
     CDS             3709..4080
                     /locus_tag="PLW82_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HOJ46289.1"
                     /translation="MKTGARPIRLSKFLWPLGFLGFLGFTYFRTGYALWLFSFSYFSF
                     FAYYFIGKMADEMPDERYMENNQKAKLKVFRVAVVALFLIGFFAGFSFVTKEMIIIAC
                     ALGWSAALIGYAFLFWHYDKH"
     gene            4094..4294
                     /locus_tag="PLW82_03085"
     CDS             4094..4294
                     /locus_tag="PLW82_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ46290.1"
                     /translation="MQKFTCNLKRYRQLAGLTQDELAHKVGVRRETIVRLEAGRYNPS
                     LKLAIDISRAVNAPIEEIFVFE"
BASE COUNT         1218 a          962 c         1125 g         1189 t
ORIGIN      
        1 gagggcttct ttacctagta cgttgccgac aactagcgag tctacaacgt tgtacagttg
       61 ctgaaatagg tcacctagga aaattggcag tgcgaacaac agaatcgatc gtgatatctt
      121 tccttcagtc aatgttttcc cttcttaccc ttttgatgta cttgtttatg atataggaaa
      181 tagtcagagc ttgcaaacac gcaagaacaa gagctgtgcg gatatttcac ttcaaaaccg
      241 attaactggt acttgtgctc tgatttggat gcggataagc tcttgcacag tggtggaaaa
      301 gcttaggtgc aggagtggtt tccatgcggg atctactaca ggacgagcag ttcatctata
      361 atatggttga tggctattcg agcgagctct ttcgagctcc tgtctcttgt gcatagatca
      421 atacgttagg agaaaaggtc atggaaaact tgttggatgc gatcctcaac atgtttaaaa
      481 gcgatgagat caggaaagtt gtgatcagca agccttcggc gaaatcgctt gagtacaaga
      541 agatcagtgc cgaaaaaaaa ggcacttact accaaatcgc taaatttaca gagactcagg
      601 tttttcacga gaatgttggt gagggagaag tcgcagaacg tgtgtatcaa ctgcttgctt
      661 ctgggttcaa gcagctgaat gcgtggtcag accgcttcga gcatatgatt ttgctgtcga
      721 aaaaaggaca atccactttc aagaaaaagg ccttgaaaga acaaacaccg gtatcaatag
      781 atgccaagca caaccgagag aagaactact taatcaaaga aggaaagcct gttgaacctc
      841 tggtggatat gggagttctt actcctgacg gaagagttgt taaagccatg tacgataagt
      901 acaagcagat caaccgtttc ttggaaatca tcgacgatga ggtagtggcc tttcaaggca
      961 aggacatcaa aatcatcgat tttggctgcg gcaaatcata tctgacattc atcctgtact
     1021 actatctgac ggaaatcagg aagatcccag ccactatcat cgggctcgac ctcaaagcag
     1081 atgtcatcga aaagtgcaat gcagctgcaa ggaagtacgg gtacaccggg cttaggtttg
     1141 aggtaggaga tatcagccag tttgaagctt cgctcccagt agacatggtt atcactctcc
     1201 atgcttgcga cacagccact gactttgctc tgtataatgc aatcaaatgg gacgcggaaa
     1261 tgatcttttc ggttccctgc tgccagcatg aactgaacaa acagatccac acagacaacc
     1321 tgtccctttt tactcgctac ggcatcatta aagaaagatt ttcggccctg gcaacagatg
     1381 ccattagggc gaatctcctg gagtacgcag gttacaagac ccagcttctg gagtttgtcg
     1441 attttgcgca cactcccaaa aacatcctaa tccgagcggt gaaaagaaag agcacctctg
     1501 actcggtaag agagaaagct ttacaagagg tggaagacgt tatgcgagag tttaacttca
     1561 atcccacact gtacagtctt gtggttgggg gagagactct aaaagggctt gtagacggca
     1621 actgttgcgt acgggcaccg cacgagaagg actgaaagaa gacgctgtta gtcattaagt
     1681 tatgcaaaaa gacgcctgcc aagagcaggc cttttttgtg agggggatta ctgccttact
     1741 tcacctgctt tcgtcaaagg attatcaatt taggcgaaaa aacacaagtt acagaggatt
     1801 tgctcacttc acattgaatt tacaaggaac ggtattgctt acaaatttac agctgttgtt
     1861 tgaagcatac aaaaaaacta aaacgtgctt tggtgagagg tttcaaatgg acttaagaac
     1921 agtacttcta atgctaacgg ttggttcctt cttgtttggt cttctccttg tgcggttcaa
     1981 gttcgataga gaaaatcctc gggaagtgcc gtattggatt gctgcaaagt ttcttcaggc
     2041 cactggttct ctcctcttgt atctgggtat gggcggaccg cttatgcttc tggcaaagac
     2101 ggtcttgatt ctggggtgtg cccatgagac atgggctatc gctattatct caggcaagaa
     2161 aatcaaaagg tggttgcatg ctgtcgtgga tttggccatt atagccgtct gcgcactgat
     2221 agcactagct agcgagacgt tcaggagcag tgcgattttc tcgctgcaga gcatttttta
     2281 cttcctgcct tttgggtttt tggttcataa accggacagg aagttctctc tacaggtgcc
     2341 tctaggtgtg ctatatcttc tgacagggct ggttttttta gttaacgccg caatgctgtt
     2401 cttctttccg ggatatggct taagtggcgc caatgtcctg ctcggcatga taccgatcat
     2461 cagcttttgc gtctttttag ccagcggatt tatccttctc atgctttcca aagaaagaag
     2521 cgacttgcag atgcagcaga tccagaagtc gctcaaggaa acggaagata ggttccgatt
     2581 gatcgtcgag accgctatcg agggtattat ggtgtttgac gaacactaca gaataacttt
     2641 cgccaacaag aacatggcgt cgatgctaga gtatacggtt gaagaaatgc ttggcaggtc
     2701 gtacagctct ttttttgccg aagaggatca ggatatctac cgggaacagg aagccctgcg
     2761 aaaaagcggg agagactcgg tatatgaatg cggcctccta accaaaagcg gtaagaagca
     2821 ctggtttttg atctccgcta gaccacttct cggacagtcg aacagattcg aaggttcttt
     2881 cgccatgctc accgacatta acgaaaggaa ggtaatggaa atggcattgg cagaagcaaa
     2941 ccgccagctt atagagctga gcaacaggga cggtctcacc gggatctcta atcgcaggcg
     3001 ctttgacacc ttcttggaaa gtgagttctc cagactgaag gcagaaaacg ctcatctgtc
     3061 catcatcttc ttggatgttg accacttcaa agcttacaac gacctctacg gccacttgat
     3121 gggagatgag tgcctcaagc agattgccaa cgtactcgca aattgcgtaa cacgaccaga
     3181 tgacctagta gcccgatacg gaggagaaga atttgcctgc gttctccctg acacagatct
     3241 tgccaatgct gtcttgaaag cagaggagat tcaggagaaa gtcatggagc tagctataga
     3301 acacaagggg tctaaggttt ccgatcttat tactgtaagc tttggtgtgg caacatttaa
     3361 ctctgcatcg ggagtcacca tcgaagagat gatgaagttt gccgacaaac agctgtacaa
     3421 ggctaagtcc ctcggtcgca accgcatcga ggcggcaaag tggcctgggc atagcgttgc
     3481 ttagctgttc cgccagcagc tatttgtgga tgtggttctt tacacatcca cttttctttt
     3541 ccaacctgct gtacggagaa gcaaagccgc tttttctagc ctgcaggcgg acctcggagc
     3601 gatgtgtttc agtacgcaga gacctcttgc aagagttgac gcgcaccgga tgatgtgata
     3661 tatttgtaac aaatgataca aatatatcac gttttggagg tcggactaat gaagactgga
     3721 gcgaggccta tcaggctttc gaagttcctt tggcctttag gctttttggg ctttctaggc
     3781 tttacctact tcaggactgg ttatgcttta tggctgttca gtttcagcta cttcagcttt
     3841 ttcgcatact acttcatcgg gaaaatggca gatgaaatgc ctgatgagcg ttacatggaa
     3901 aacaaccaaa aggcaaaact gaaggtgttc agagttgcgg tggttgctct gtttctcatc
     3961 ggcttttttg ccggcttttc ttttgtcact aaggaaatga tcatcatagc ttgtgctttg
     4021 ggctggtcag ctgcattgat cggttacgcc tttttgttct ggcactacga taagcactaa
     4081 aggagtgatg cttatccaaa agtttacgtg caatctgaag agatatcgcc agctcgcagg
     4141 gctaacccag gacgaactcg cccacaaggt aggggtcagg agggaaacca ttgtgaggtt
     4201 ggaagctgga agatataacc catcgttgaa actggctatt gacatttctc gggcggtgaa
     4261 cgcccctatt gaggagattt tcgtgtttga gtagagtaca gagatcgagg aactggagtt
     4321 ttctgtacag attccctaag aagcctaagg agcttttagc aggcacagag ctcctgctgg
     4381 gtaagaggat tctacgatgt caatgggagc taaacactag caagggattt gaggaaggag
     4441 atgcgttaat ggtatttgta tgctacccca agtgcaccac ttgccaaaag gcaa
//
LOCUS       DAOSDA010000091         4469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12277_length_4469_cov_3.661531, whole genome shotgun sequence.
ACCESSION   DAOSDA010000091 DAOSDA010000000
VERSION     DAOSDA010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12277_length_4469_cov_3.661531"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PLW82_03090"
     CDS             complement(<1..721)
                     /locus_tag="PLW82_03090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOJ46291.1"
                     /translation="MKLPQSFILEMEALLGEKLPGFLHAVTEESARPGLRVNTLKIAV
                     DEFLERSPWPLEEVPWCPEGFYYPEDARPAKHPFYHAGLFYLQEPSAMAPVVILDPQP
                     GDRVLDLCSAPGGKTLQIAARMKGEGVIVANDVYPDRLKALSRNVEQAGVRSAAVLQE
                     SPLNLQKRFAGYFNKILIDAPCSGEGMFRKDPRGIKAWEEYPPEFCQKRQRGILEEAS
                     TLLQPGGVLVYSTCTFNRSENE"
     gene            811..2943
                     /gene="pcrA"
                     /locus_tag="PLW82_03095"
     CDS             811..2943
                     /gene="pcrA"
                     /locus_tag="PLW82_03095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HOJ46292.1"
                     /translation="MNSELLLKNLNPQQQQAVQHHTGPLLIIAGAGSGKTRVITHRIA
                     YLIAHYKVKPMNILAVTFTNKAASEMQDRVKGLIGPVADFVTISTFHSFCARLLRIEG
                     EHIGIKRGFTIYDANDSENLAKKVLSELNLDPKRFPEKMMLGAVSTAKNNLILPEDYP
                     EDSFTEEKIKQFYKLYQSKLRESNAVDFDDLIVQTVLLFKKHPEILKKYQDRYQYIMV
                     DEYQDTNHTQYILVALLAADHKNICVVGDDDQSIYSWRDADIRNILEFEKDFPNTTTI
                     KLEQNYRSTCRILEAANSVISHNLNRKPKTLWTENPKGEKVIVYRAASERHEAQFIAD
                     EIKRLTKKGVRLKDIAILYRMNSQSRVIEEVFLNNNLPHVIYGGIRFYDRAEIKDTLA
                     CLRLIANPYDTISFNRAINTPRRGIGPATIAKIEQSKEMLGLSYLDTLLQAKSIPGLG
                     GKLKVLTEFAENWYHLTATQDQLTITELVESIWKTSGYMEMLENEGTDEALSRIDNLK
                     EFLSVTKSYDERAKDQGSLNGFLEEISLISDIDRYSDESNAVSFMTIHSAKGLEFPVV
                     FVTGLEEGLFPSSRSLYDEHQMEEERRLCYVAITRAMHLCYLTYAETRFLWGQTSYNM
                     PSRFLSELPDEADDSDTEKAAVSVKTNSGQPSPDLKSGDKVVHEKFGKGIVIEVKGEG
                     ASREAAVVFDSSGLKKLLLEYAKLKKID"
     gene            complement(2970..3590)
                     /locus_tag="PLW82_03100"
     CDS             complement(2970..3590)
                     /locus_tag="PLW82_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46293.1"
                     /translation="MTSKAYKAACRYILGLLDAKAEFSVALNSVIAYCQKQFPHENFE
                     VFRNERLINEEIEYLYKWFGAIIEESPPPEEIAILWFYIVTENVAGEYSMDFCFAGDR
                     KQEPKRFGDGLLESPEYLPNNHLAHSSLLESWADAFENLPFESSFFVEYMLGMTYGSF
                     LARDLAIRFADKLTAASPRIVKAGFRLGDLVKVGKIENGVFTRSIE"
     assembly_gap    3861..3870
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1254 a         1152 c          937 g         1116 t
ORIGIN      
        1 cttcgttttc gctccggttg aaagtgcagg tcgaatagac cagcacgcct ccaggctgca
       61 gaagggtact tgcttcttcc aaaatgccac gctgcctctt ctgacagaac tccggcggat
      121 attcttccca ggcctttatg ccccgaggat ctttgcggaa catgccttct cctgagcacg
      181 gtgcatcgat aagtattttg ttgaagtaac ctgcaaaacg tttctgcaga ttaagggggc
      241 tttcttggag caccgctgcg cttctcacac cggcctgctc tacgtttctg ctcagggcct
      301 ttaaacgatc ggggtaaaca tcattggcaa cgatgacacc ttcacccttc attctagctg
      361 cgatctgcag ggttttccct ccaggagcac tgcagaggtc cagcactctg tcgccgggct
      421 ggggatccag gatcaccaca ggtgccatag ccgaaggctc ttggaggtag aacagtccgg
      481 catggtaaaa ggggtgtttt gccggtcttg cgtcctcagg ataatagaag ccttcaggac
      541 accacggtac ttcttccaaa ggccagggag agcgttctaa aaactcgtct acggcgattt
      601 tcaaggtgtt aaccctgagc ccgggtcttg cgctttcttc agttacagca tgcaaaaacc
      661 caggcagctt ctcccctaaa agagcctcca tctctaaaat aaaactttgc ggtagtttca
      721 taatgtatct tcctatcatc agtttgtatt taccctattt tgcagggtta gaaaaagtgg
      781 tggcgaaaaa gccaagtgga ggtgtggctt ttgaactctg aactattact gaaaaacctc
      841 aatccccagc agcaacaagc agtacagcac cacacaggtc ctctgcttat cattgccgga
      901 gccggcagcg gtaaaactcg ggttatcacc caccgcatcg cctacctgat cgcacactac
      961 aaggtcaaac ccatgaacat ccttgcggtt accttcacca ataaagcagc cagtgagatg
     1021 caagatcgcg ttaagggtct cattggtcct gttgcggatt tcgtaaccat cagtactttc
     1081 cactcttttt gtgccagact gcttagaata gaaggcgagc acatcggcat caagagggga
     1141 ttcaccatat atgatgcaaa cgactccgaa aacttagcga aaaaggttct gtcggaactg
     1201 aatcttgatc cgaagcgctt cccagaaaaa atgatgctgg gcgctgtgtc tacggcaaaa
     1261 aacaatctta ttcttccgga agactatccc gaagacagct ttaccgaaga gaaaatcaag
     1321 cagttttata aactttacca aagcaagctc agagaaagca atgcagtgga ttttgacgac
     1381 ctcatcgtcc aaacagtact gctctttaag aagcatcccg agatcttaaa gaagtaccag
     1441 gatcgctacc aatatattat ggtggatgag tatcaggaca ccaaccacac tcagtacatc
     1501 ctcgttgctc tccttgctgc agaccacaag aacatctgcg ttgttggaga cgacgaccaa
     1561 tccatttaca gctggcgcga tgcagacatc agaaacatcc tcgagttcga aaaggacttc
     1621 cctaacacta caaccatcaa gctggagcag aactaccgtt ccacctgcag gattctggaa
     1681 gcggcaaata gcgtcatttc tcacaaccta aaccgcaaac ctaaaaccct ctggactgaa
     1741 aaccccaaag gcgagaaagt tatcgtgtac agggcagcct ccgaaaggca cgaggcacaa
     1801 tttattgcag acgagatcaa acgactaaca aaaaaaggag ttcgactcaa ggatattgca
     1861 atcctttacc gcatgaactc ccagtctcgc gttattgaag aagtgttttt gaacaacaac
     1921 ttgccccatg tcatctacgg cggtatccgt ttctacgatc gggccgaaat caaagataca
     1981 ctggcttgtc tccggttaat tgccaacccc tacgacacga tttccttcaa tcgcgcgatc
     2041 aatacaccga gaagaggaat cgggcctgcg accatcgcca agatcgagca gagtaaagaa
     2101 atgctcggtt tgagctattt ggatacgctg ctacaagcaa aaagcatacc cggactgggc
     2161 ggcaaactaa aagttcttac tgagtttgct gagaactggt atcacttgac cgcaactcag
     2221 gatcaactca ccatcacaga actcgtagag tccatttgga agaccagcgg gtacatggag
     2281 atgctggaaa acgaaggaac cgatgaggcg ctgtctagaa tcgacaactt aaaggagttt
     2341 ctcagcgtaa caaaaagcta cgacgaacgg gcaaaagacc aggggtcctt gaacggcttc
     2401 ctggaagaga tatccctgat tagcgatata gatcgctaca gtgacgaaag caatgccgtc
     2461 tccttcatga ctatccactc agccaaaggc ctggagtttc ccgtagtgtt tgtaaccggt
     2521 cttgaggaag gtctcttccc atcgtctcgc tctctatacg acgagcacca gatggaagaa
     2581 gaaagaagac tctgctacgt agccatcacc agggctatgc atctctgcta cctgacttac
     2641 gccgaaacca gatttctctg ggggcagact tcctacaaca tgccttcccg ttttctcagt
     2701 gagcttccag atgaagcaga cgattccgat acagaaaaag ctgctgtttc ggttaagacc
     2761 aactcgggac agccctcacc tgatcttaaa agcggtgata aagtggtcca cgagaagttt
     2821 ggaaagggca tcgtcataga ggtgaagggc gaaggcgctt ctcgggaagc tgcggtcgtg
     2881 ttcgacagtt caggattgaa aaaactgctt cttgaatatg ccaaacttaa aaagatagat
     2941 taaggcggta aagagcaact ctttaccgcc tattcaatgc tgcgagtgaa tacaccgttt
     3001 tcgatttttc ccactttgac caagtctcca agccggaacc ctgctttcac aattcgcgga
     3061 cttgctgctg taagcttgtc ggcaaaacgt atcgcaaggt cccttgctaa gaagctgccg
     3121 taggtcattc ccagcatgta ctctacaaaa aagctggact caaaaggcag attctcgaaa
     3181 gcgtctgccc aagactcaag gagactagaa tgagccagat ggttgtttgg caggtactct
     3241 ggggattcca agagaccatc gccaaacctc ttgggctctt gttttctgtc tcctgcaaaa
     3301 caaaagtcca tactgtattc tccggctacg ttttccgtga caatatagaa ccacagaatt
     3361 gcaatctcct ctggaggcgg agactcctca ataatcgctc caaaccattt gtacaggtat
     3421 tctatttctt cgttgataag cctttcattt ctgaagactt cgaagttctc atggggaaac
     3481 tgtttctgac agtaggcaat gacgctgttc agtgcaacag aaaactctgc ctttgcatcc
     3541 agaagaccca gtatgtaacg acaggccgct ttgtaagcct tgctggtcat ttcagtagcc
     3601 cccttagctc tttagccaag cctttagtct ctttcttcag cacatccagg tcaagtcctg
     3661 ctcaaaaaca gtatactatg ttgtgatata tgtaggcgaa aaaagtttta aaaaaggtgt
     3721 aacttttttg gggtctctcc gttatactat cgaaaacaga tgaaagccaa aatgctttta
     3781 ttcgtttcat ttatttccaa caccctttat atacagatgc cggtaacccc ccggcatcga
     3841 aaccgcaaac caatcccttt nnnnnnnnnn aacgttagga actggtttcg aaaaaaacca
     3901 aaaaactttt cgttttaggt gtaacttttt cgccccccct accgttatat aggtgaaact
     3961 caaaaccaac aactaatccg atgcaattca cttaacattt tataccccaa cacccttttc
     4021 atttggatgt cggccacccc cgacatccaa ccccttggat cttaaatgtt ttcatatata
     4081 catttataaa acttttattc ttatttccct gatggaagag taagcagtta ctcttccatc
     4141 cacccacctt tattctttga ttcttaaccc caacatcctc cacccaataa ggttcgcaag
     4201 aagctcgcct tcacgaacct aggtagatac cacctaccgt tctgctttct gtcaaagaaa
     4261 cgcttgggta aaaccaagtg tttctttatg tggaaagcca gaaaacaaaa ttaggtgtaa
     4321 ctatttgggc tttgcaccgt tatatgtgca ccggaggaac acaaccaagc aatcacctaa
     4381 atttttatat atcccaacac cctttttcat aaagatgccg gttacccccc ggcatctccc
     4441 cccaccccaa catactccca taactcaat
//
LOCUS       DAOSDA010000092         4402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12515_length_4402_cov_3.564067, whole genome shotgun sequence.
ACCESSION   DAOSDA010000092 DAOSDA010000000
VERSION     DAOSDA010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12515_length_4402_cov_3.564067"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..739
                     /locus_tag="PLW82_03105"
     CDS             149..739
                     /locus_tag="PLW82_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46294.1"
                     /translation="MLKRYKEVYRLVLENASDQEVITALADVLGLPVELNTAENIDRS
                     NSVALPVPITNGEDPPYYLVVKAPSIDDNAAILVESVASLLGRRAVLRLVRDADADRS
                     VRMQVSIAVASLSMAELIAVKHLLGQLKGREGVIVASEIADELNISRSSVASALEKLC
                     AAQVMQKFSLGRKGTFIRVTTPQLFDEVAKLAPRNI"
     gene            complement(814..3144)
                     /locus_tag="PLW82_03110"
     CDS             complement(814..3144)
                     /locus_tag="PLW82_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46295.1"
                     /translation="MLKYVLLVCCFFLLTQVGVGAADLIVDDFRDVSDWLGDFAVDGG
                     KLTVKGEATKQVLQDWSGYLYLEIARTEDSPLEIRIVDGDGEVWTAQGSEKTIAIPFS
                     EFELIGSTSAGDKILSTGKVQTLTLKGNTQLNFIGLSGSKGCAGPNVYIQTANKYYAG
                     KTWEKAAEEIKNKGYTAVHLTPVELTPSTYYKQKEMVDAFHKAGLPVALTIYPGTDFW
                     AYNAYPQWHQSFLKGGGSKWSWRVYNCLREEEFIDYTIKYLQEQFLEYGYDALHISEP
                     WLEVWGGPNNPELYACFCDRCRSAFENETGVDPVELFNNRSQYYYTKNPELYQKWVDF
                     RVDTVASFVDKIIKGLREVKPDVPVYVMFLSDVTVEPGKTREYQAMDLERFAEIGDGV
                     IIETAWQDWTRSDLWPGYILSYGMEYSPRVFAANPDALIFAQPDVGSNWDEMHRSPQW
                     LLDFSAYAYRTGFDGYIVYEYSQMGYRMLPSTEIILDDFEKTESGNYWTSVKSGFDEV
                     LVGRASNNAFEGEGSLLIYYSGVISGLSGAEKDVMLDLSGFEYFDLQINFSTLSSGET
                     VSLQYEIVTDRNTYSAQESVAVQEGWQNVRLPFSKLGEDLNLSHVRKIRISLVKTEQL
                     DDFGMIYIDLLKVGGSRSSFIESYNLPRTSSPKPASKLEIGDMKIEPLGSTRLESGLN
                     QVDVKVTLVSGERSIGSVVFDLVVRDATSKQEQPKLSRTYRVGALRRETEREVMTQLI
                     LPDGEFEIFVRAYTQGLDFVSEKSDLAISESINLKF"
     gene            complement(3164..3502)
                     /locus_tag="PLW82_03115"
     CDS             complement(3164..3502)
                     /locus_tag="PLW82_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46296.1"
                     /translation="MTDRIIQAQDNSIYIGQVLLGFTVGRAKFYSLTQIVLEKGLHHG
                     KVVLLDPSGKALSDMEFPNMYAQTDNTLFSMAVLWDIPFKKPGDYFIAIYLNEVKVGQ
                     YKFIVVDKVE"
     gene            complement(3531..3947)
                     /locus_tag="PLW82_03120"
     CDS             complement(3531..3947)
                     /locus_tag="PLW82_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46297.1"
                     /translation="MKNSREERLRLLTEGVVQELHHKRAVARTRIPAIAAALVLLTFG
                     IYFYTSMFMMGVTVQNAGVIGSTISYVIDDEAPQMYVQAHNVSVIGSHSTREATSRSI
                     VSSVVRKGEVQYNGQSQQDSVSVSPSHIIITTSTSL"
     gene            complement(4120..>4402)
                     /locus_tag="PLW82_03125"
     CDS             complement(4120..>4402)
                     /locus_tag="PLW82_03125"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ46298.1"
                     /translation="DEVANTIVELAGASSDSFVEGFEDKEVMEAVLRILYDDDFNPDQ
                     RDAILYFFVQELDQRTISEITGAKIGTVKKRIHDGLKKLRQKLAKEDLI"
BASE COUNT         1122 a         1097 c          937 g         1246 t
ORIGIN      
        1 tacgctgagt tcatctaacc gagggatttt acacacactt atggacttga tcttctgcat
       61 tagatgttat aaatgaaaaa caatgtggta taataacttt gaatcttata gttctcctta
      121 ggtcctaatt ctattaggag gtagaagtat gttaaaacgt tataaggagg tttacaggct
      181 agtccttgag aatgcttctg atcaggaagt gataactgct ttagctgatg ttctcggctt
      241 gccagttgag ttgaatacgg ctgaaaatat agacagatcc aactccgttg ccctcccagt
      301 cccaattaca aacggagaag atccaccata ttatttggta gttaaagctc ctagcattga
      361 cgataatgca gctatcttag ttgagtctgt agcaagtctg ctcggaagga gagcagtcct
      421 gcgcctcgta agagatgcag atgccgacag atcagtaaga atgcaagttt ctattgctgt
      481 agcgtcgctt tcgatggccg aactgatagc tgtgaagcac cttctcggtc aactgaaagg
      541 aagagaaggc gttatagtag catcagaaat agccgacgag ctaaatatct cccgctcatc
      601 agttgcaagt gccttggaaa agctttgtgc agcccaggta atgcaaaagt tttcactggg
      661 aagaaaaggc actttcatcc gtgtaaccac tcctcagctt tttgatgaag tagcaaagct
      721 tgcgcctcga aacatttaag ccaaacaacc tctctaccgc tctccattat caataaaagc
      781 agcctgcgtt tgcaggctgc ttttattgtt agtctagaac ttcaaattta tactttcgct
      841 tatagcaaga tcgcttttct cactgacgaa atccaaccct tgggtataag cccggacgaa
      901 gatctcaaac tcgccgtcgg gcagtatgag ctgagtcatt acttctcttt cggtttccct
      961 tcttaatgct ccaaccctgt aggtacgact gagctttggc tgctcctgtt tggatgttgc
     1021 gtcccgtaca accaggtcaa aaactacgct gccaatggat ctttcccctg ataccagagt
     1081 aactttcaca tcgacttggt tcagtccgct ttccagacgg gtgcttccta aaggctcgat
     1141 tttcatgtcg ccgatctcta gtttcgacgc aggcttaggc gaacttgtcc ggggtagatt
     1201 ataggactcg atgaacgaac tccggcttcc accgactttc agaagatcga tatagatcat
     1261 cccgaaatca tcaagctgtt ctgtctttac cagactaatc ctaatcttcc tcacgtgtga
     1321 aaggttcagg tcttcgccta gctttgagaa aggcagtctt acgttttgcc agccttcctg
     1381 caccgcaacg ctttcttgtg cagagtaggt attacggtct gtaactattt catactgcag
     1441 agacaccgtt tctccagagc ttaaggtaga gaaattgatc tgcaggtcga aatactcgaa
     1501 acccgataga tctaacatca cgtctttctc ggcacctgaa aggcccgaaa taacgcctga
     1561 gtagtagatg agaagactgc cctcgccttc aaaggcattg ttactggctc taccaaccaa
     1621 aacttcatcg aatccagact tcaccgatgt ccagtaattt ccggactctg ttttttcaaa
     1681 gtcatcgagt ataatctctg tggatggcag catgcggtat cccatctggc tgtactcgta
     1741 gacaatgtat ccgtcgaagc ctgtgcggta tgcataggca gaaaagtcaa gcagccactg
     1801 tggggatctg tgcatctcat cccagttgga ccccacgtct ggttgggcga aaatcaaagc
     1861 gtcaggattt gctgcgaaca ccctcggcga atactccatt ccgtatgaga gaatgtaacc
     1921 tggccaaaga tcggatctag tccaatcctg ccacgcggtt tcgatgatga ctccatcgcc
     1981 gatctcagca aatctttcaa ggtccatcgc ttgatactct ctggtcttcc ctggttcaac
     2041 tgtaacatca gaaaggaaca tcacgtaaac cggcacatcg ggctttactt cccgcagccc
     2101 tttgatgatt ttgtcaacga agctcgcaac ggtatctact ctgaaatcaa cccatttttg
     2161 atagagttct gggtttttgg tgtagtagta ttgggatctg ttgttaaaaa gctctacagg
     2221 gtctactcct gtttcatttt caaacgcact gcggcaccgg tcgcagaaac aggcatacag
     2281 ttctgggtta ttcggtcctc cccagacctc aagccagggc tcgctgatgt gcaaagcatc
     2341 ataaccgtac tccaagaact gctcttgcag atatttgata gtatagtcga tgaactcctc
     2401 ttcccgcaga caattgtaca ctctccagga ccacttgctt cctccacctt tcaaaaagct
     2461 ttggtgccac tgagggtacg cattatatgc ccaaaaatca gtccctggat agatcgtcaa
     2521 ggccacgggc agtcctgctt tgtggaaggc atcgaccatc tctttttgtt tgtagtacgt
     2581 gcttggcgtc agttccaccg gagtcaggtg aacggcggtg tatcctttgt ttttgatctc
     2641 ctcagccgct ttttcccacg tcttgccggc gtagtacttg ttcgctgtct gaatgtaaac
     2701 attcggtcca gcacagcctt tagagccact caaaccgatg aaattcagtt gcgtgtttcc
     2761 ctttagagtc agcgtctgca ccttaccagt ggacaggatt ttgtctcctg ccgatgttga
     2821 tccgatcagt tcaaactcgc tgaatggtat agcgattgtt ttttcactgc cttgagcggt
     2881 ccaaacttca ccgtccccgt caacaatccg gatctctaga ggtgagtctt cagttcgagc
     2941 aatctccagg tacaagtagc cactccagtc ttgcagcacc tgcttggtcg cctctccctt
     3001 tacggtaagc ttgccaccat caactgcaaa gtcccccaac cagtccgaaa catccctaaa
     3061 gtcgtccaca atcaaatctg ccgctccaac tccgacttgc gtcaggagaa aaaagcagca
     3121 gaccaaaagc acatatttta gcatcttgcc accccatttc cgtttattct actttgtcta
     3181 ctacaataaa cttgtactgt cctactttga cttcgtttag gtaaatggcg atgaagtagt
     3241 ccccaggctt cttaaaaggt atatcccaaa gaacggccat ggaaaacagg gtgttgtcgg
     3301 tttgggcata catattgggg aactccatat ctgagagggc tttcccactg gggtctagca
     3361 gaaccacctt cccgtgatgc agacctttct ccaggactat ttgggtcaga gagtagaact
     3421 tggccctgcc cacggtaaaa ccgagcagaa cttgcccaat atagatgcta ttatcttggg
     3481 cttgaatgat tctatcggtc aagacatggt tgatgatgat gtcgctatta ctataggctc
     3541 gtgctggtag taataatgat atggctagga gaaacagaca cactgtcttg ctggctctgc
     3601 ccattatact gaacctcccc tttacgcact acagatgaaa cgatcgatct ggatgtagct
     3661 tctcgggtcg agtgagatcc gatgacacta acattgtgtg cttgtacata catctgtgga
     3721 gcctcgtcat caatcacgta cgaaatagtg gatccaatta caccggcgtt ttgaactgtt
     3781 acacccatca tgaacatgct ggtgtaaaag tagatgccaa atgtaagcag aaccaacgca
     3841 gctgcaatgg caggtattcg agttctcgca acagcacgtt tgtgatgaag ttcctgaact
     3901 actccttcgg taagtagtct caatctctct tctctagagt ttttcacagt ctgtaacact
     3961 ctaactgctt tgttcaaaga agaaagctca ttttgacagg aagtgcagct tgaaagatga
     4021 tctgtgtaca ccgaagctct gtgttctttt tgagacataa gctctagcag ctcatattcg
     4081 cttaagtggt tgctttttct catataaatc tccctcatct caaatgagat cctctttcgc
     4141 caatttttgc ctcaacttct tcaaaccgtc atgaatgcgc ttcttgaccg ttcctatttt
     4201 tgctccagtg atctcagaga ttgtccgctg atccaactcc tggacgaaaa agtaaaggat
     4261 ggcatcccgc tgatcaggat taaaatcgtc atcgtaaagg atccttagca ccgcttccat
     4321 tacctcttta tcttcgaagc cttctacaaa actatcgctt gaagctccag ctaattcaac
     4381 gatagtattg gcaacttcgt cg
//
LOCUS       DAOSDA010000093         4392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12544_length_4392_cov_3.417109, whole genome shotgun sequence.
ACCESSION   DAOSDA010000093 DAOSDA010000000
VERSION     DAOSDA010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12544_length_4392_cov_3.417109"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1219)
                     /locus_tag="PLW82_03130"
     CDS             complement(266..1219)
                     /locus_tag="PLW82_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HOJ46299.1"
                     /translation="MTKKVWFAGILSLVFVVIFSFVAMADDSIILTGKWSFQFDDDLA
                     WADPEYDVSDWETYTNPADAPALGFGWYRLDFEVPAEWEGKGDLELSIAELYDFDWSF
                     VNGIGVGNGNRSGNRKYSVPASYLNYGGKNTLALRIYTINPGIGLDGEIKLSLTGVVS
                     MVKNPSFEQAVNLPLGWTPTRTDKNIFIWENAATVRTGFRALALKGTDALEGEEAAWM
                     SDIILVKPGTEYRAQAYVKAEDPTGETTIQIRFYNADGDEVFYDGERAVSRNLARRTA
                     DWTRGNLFGTAPEDAVYARIFLVSNDNSGIVWFDDVSFGAE"
     gene            complement(1389..2513)
                     /locus_tag="PLW82_03135"
     CDS             complement(1389..2513)
                     /locus_tag="PLW82_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46300.1"
                     /translation="MAEVVLKNVTKQYPNGVVAVDNINLTIDDEQFLVLVGPSGCGKT
                     TTLRMIAGLEEITQGEIMIDGKVVNKIPPDERDIAIVFQDYVLYPHKTVYENMAFGLE
                     NSGLREVEAQRAIEEAASILGLSGLLDRFPGELSGGQKQRVALGRAIVRQPKVLLMDE
                     PLSNLDPVLRSEMRAHIKRMQRKRKITTVYVTHDQQEAMTMGDKIVLMHNGVIQQEGP
                     ARELYLKPRNKYVATFLGNPSMNIFDAKLVKEGDKVYAVADSLKVKLLPEHASQAGVQ
                     ERIGKEVFLGLRPESLEDVEFVTEIDVDPANIVTVTVDLLEAVGASAYVTWYVGNNKF
                     VAEVDAHSSARVGQEVKLYADPRGVQLFDKVTEESILVRD"
     gene            complement(2545..3693)
                     /locus_tag="PLW82_03140"
     CDS             complement(2545..3693)
                     /locus_tag="PLW82_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46301.1"
                     /translation="MVTVRLENIEKVFEQKAPSGEVSKVYALKNINLTIHDGETIAVV
                     GPSGCGKSTLLRVIAGLERPTDGNVYFDDKVVNDVEPKDRGIGIVFQNYALYPHFKSK
                     GNLSFFFKLRRVKEDVINERVRITAETLGVGFEELLNRKPGQLSGGQQQRVALGRCII
                     KEPTIFLLDEPLSNLDAKLRERTRIELKRLLRRFKVTTFYVTHDQVEAVTLGDRIAVM
                     RSGQILQVDPYREIYKHPINMFVANFIGLPPMRFLDVELKGQKLVGNGFEVEISSDVV
                     KAIGDESRKLAAGIRPEHVEIKTTPEPGFFEATVDVIEPLPNEGANYVYFSFAEKAES
                     IAKTDLNWEGHVGDKIYVKLDPEKIYVFDKETEETIVAQDKIFSVVNA"
     gene            complement(3716..>4392)
                     /locus_tag="PLW82_03145"
     CDS             complement(3716..>4392)
                     /locus_tag="PLW82_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ46302.1"
                     /translation="FSDLALAMGSTDTPFIRYMFNSAFVSTVVVIGTIVISTLAAYPL
                     AKHNFPGRNLIFQVIVSALMISSHVTMIPRYLVIRNTGWIDTYYALIIPALAGSYGLF
                     LMKQFIEPIPNEMLESAKMDGASEWKIFTNIILPLVKPAWATLSIFTFISTWNDFFTP
                     LIMTKSDAMKTLPLAMRTISDGLTIARMGAAAAGSFLTTIPVILVFIFFQRYVVETMT
                     YSGMKG"
BASE COUNT         1145 a         1057 c          960 g         1230 t
ORIGIN      
        1 ccatcaacgc catcagtagc agcaacacca aatagcaccc taaaatcgac gcaaagagcc
       61 caagaccggc tatatatcct gcgatgagtg ccgatttttg gtctgcaact gcggccaggg
      121 ctgcccgcct gtagttgtca taatctttat cgcgaatctt gcgcacagat ccacctccac
      181 aggacagctg tcaaagcagc actaatttta agaaaccttc tcctcctata ggaggagaag
      241 gtttttattg cgtcagctta tgattttatt cagcgccaaa ggacacgtcg tcaaaccaga
      301 cgattccact gttgtcgttg gataccaaga agattcttgc atatactgca tcttcaggag
      361 cggtaccaaa caggttgcct cttgtccagt cagcagtacg acgagccaag ttgcggctta
      421 cagctctttc tccatcgtag aagacctcgt cgccatctgc attgtagaag cggatttgaa
      481 ttgttgtttc gccagttgga tcttctgctt ttacgtaagc ttgtgctcta tactctgtgc
      541 ctggtttaac gaggataatg tcgctcatcc aagcagcctc ttcgccctct agagcgtcag
      601 tacctttgag agccaatgca cggaaacctg tgcgtactgt agctgcgttc tcccagataa
      661 agatgttctt gtcagttctt gttggtgtcc agcctaatgg caggtttaca gcttgctcga
      721 agcttgggtt cttaaccatg ctgactacgc ctgtgagact aagcttaatc tcgccatcga
      781 gaccgatacc tgggttgatg gtgtatatac gaagtgcgag tgtgttcttt ccgccatagt
      841 tcagatagct agctggaaca gagtactttc tgttgccaga acggttgccg tttccaacac
      901 cgatgccgtt aacaaatgac cagtcgaagt cgtagagttc agcgattgaa agctcgagat
      961 cgcctttgcc ttcccattct gctggtactt caaagtccaa tctgtaccaa ccgaaaccaa
     1021 gagcaggtgc atctgcagga tttgtgtaag tctcccaatc acttacatca tattctggat
     1081 ctgcccatgc tagatcgtcg tcaaattgga aggaccattt cccggtaaga atgatagaat
     1141 cgtctgccat tgcaacaaag ctgaagataa ctacaaacac taatgacaga ataccagcaa
     1201 accaaacttt ctttgtcatc ttatctcccc tccgtcaata taatcctaga atcttgaccc
     1261 ccaacataga ggctagtatt tttgtcatcg gcatttgcgc tagacaaaaa tataggcctt
     1321 agttaaaaaa tactttacaa ggggctaaat accttctaat attttagccc caaagcaaac
     1381 atttttagtt agtctctaac caagatggac tcctcggtaa ctttgtcaaa cagctggacg
     1441 ccacgaggat ctgcgtagag tttgacttcc tgaccaactc ttgcagatga gtgagcgtca
     1501 acttcagcaa caaacttgtt gttgcctacg taccatgtga cgtaagccga agctcctacg
     1561 gcctccaaaa gatccacagt aacggtgact atattcgctg gatcgacgtc aatctcggtt
     1621 acaaactcga cgtcctctaa ggactccggt cttagaccga gaaatacttc cttgccgatg
     1681 cgttcttgaa cgccagcttg agatgcatgt tcaggcaaga gtttgacctt caaagaatcg
     1741 gctacagcgt ataccttgtc accctcttta acaagcttcg catcaaagat gttcatgctt
     1801 ggattgccaa ggaacgtagc aacgtactta ttcctaggct tcaagtagag ttctcttgca
     1861 ggaccctctt gctggatgac gccattgtgc atcaacacga tcttgtcacc catggtcata
     1921 gcttcttgct ggtcgtgagt tacatacaca gttgtgatct ttctcttgcg ctgcattctc
     1981 ttgatgtgag cgcgcatttc agatctgaga acagggtcta ggttggagag cggttcgtcc
     2041 atcaaaagca ctttaggctg acgtacgata gcacgaccga gagcaacacg ttgcttctga
     2101 ccgccagaaa gctctccagg gaacctatcc aaaaggccgg acagacccag aatacttgca
     2161 gcctcttcaa tagctctttg tgcttctact tctctaagac cgctgttttc cagaccaaag
     2221 gccatgtttt cataaacagt cttgtgagga tacaaaacat aatcttggaa aacgatagca
     2281 atatctcttt catcaggtgg aatcttgttt actactttgc cgtcaatcat gatctcgcct
     2341 tgggtgatct cttccaaacc ggcgatcatg cgcagtgttg tagtttttcc gcatcctgat
     2401 ggcccgacta gaaccaggaa ctgctcgtcg tcgatggtaa ggttaatgtt gtcgacagct
     2461 actacaccat tgggatattg cttggtgacg tttttcaata ctacttctgc caaagctatc
     2521 tcccccctct attaaatcgt tgctttaagc gttgactact gaaaagattt tgtcctgtgc
     2581 aacgatggtt tcttctgttt ctttgtcaaa aacgtagatc ttttctggat cgagcttcac
     2641 gtagatcttg tcgcctacat gaccttccca gttcaagtcc gtcttagcga tgctctctgc
     2701 cttctctgca aaggagaagt agacgtagtt cgctccctcg tttggtagcg gctcaatgac
     2761 gtcaacggta gcttcaaaga agcctggttc aggagtagtc ttaatctcta cgtgctccgg
     2821 acgaattcct gctgcgagct tgcggctttc gtcgccaata gcctttacca catctgaaga
     2881 gatttcgacc tcaaagccgt tgccaacgag tttctggcct ttcaattcaa catccaggaa
     2941 cctcattggt ggcaaaccga tgaagttcgc tacgaacatg ttgattggat gcttatagat
     3001 ttccctgtaa gggtctacct ggagaatctg gccacttctc ataactgcga ttctatcgcc
     3061 aagggtaaca gcctctacct ggtcgtgagt aacatagaag gtagtgactt tgaaccttct
     3121 gagcagacgt ttgagttcaa ttcttgttct ctcacggagc tttgcgtcca ggttagacag
     3181 cggttcatct aacaagaaga tggtaggctc cttgataata caacgcccta aagcaactct
     3241 ctgttgctgt ccaccagaca actggccggg tttgcggttt aagagttctt caaaacctac
     3301 tccaagagtt tcagcagtga ttcttacgcg ttcgttgata acatcttctt taactcttct
     3361 tagcttgaag aagaaagaca aattgccttt ggatttgaaa tgtgggtaaa gagcatagtt
     3421 ctggaagact ataccgatac cacggtcttt tggttctacg tcgttaacga ccttatcgtc
     3481 aaagtagacg ttgccatcag taggtctttc caaaccggca atgacacgca acaacgtaga
     3541 cttaccgcaa cccgacggcc ctacaacagc gatggtttca ccatcgtgaa tagtcaaatt
     3601 gatgtttttg agtgcgtaga cctttgagac ttcgccgctt ggtgcttttt gttcgaaaac
     3661 tttttctata ttttcgagcc gtacggttac cattagaaat tcacctctta attaattagc
     3721 ccttcatacc ggaataggtc atagtttcta caacgtatct ctggaagaag ataaatacta
     3781 aaattacagg gatagttgtt aagaagctac ctgcagcggc ggcacccata cgagcaatgg
     3841 tcagaccgtc agaaattgtt ctcatagcta gcggcagagt cttcattgcg tcgctcttgg
     3901 tcatgatgag cggtgtgaaa aagtcgttcc atgtgctgat aaaggtgaag atggaaagtg
     3961 tagcccatgc tggtttaaca agtggaagaa taatgttggt aaagattttc cattcgctgg
     4021 caccgtccat cttagccgat tcgagcattt catttggaat tggctcgatg aactgtttca
     4081 tcaagaacag accgtaagat ccggccaaag ctggaatgat aagcgcatag taggtgtcga
     4141 tccaacctgt gttacgaata accaggtatc ttggaatcat cgtaacgtgg ctagagatca
     4201 tcagcgcact aacgataacc tggaagatca ggtttctacc agggaagtta tgctttgcaa
     4261 gtggatatgc tgcaagcgtc gaaataacaa ttgtaccgat aacaacaacg gtagaaacga
     4321 aagcactgtt gaacatatag cggataaacg gtgtatcggt actgcccatc gctagcgcaa
     4381 ggtcgctaaa gt
//
LOCUS       DAOSDA010000094         4385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12569_length_4385_cov_2.878753, whole genome shotgun sequence.
ACCESSION   DAOSDA010000094 DAOSDA010000000
VERSION     DAOSDA010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12569_length_4385_cov_2.878753"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLW82_03150"
     CDS             complement(<1..439)
                     /locus_tag="PLW82_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ46303.1"
                     /translation="MRWGLSLSGGSLRGAAHIGAIKVLDRYGLRPSAIAGTSAGSIVA
                     ALYASGLTGEQMEAFALSLDPKKVFDKAIKAWQYPLVLAKIIGDFAGFNPKIKAPDGL
                     FKGDYIAEEIAKRTGGKRFSDLQMTTIINAVDLDTGDEVVFTNG"
     gene            568..1413
                     /locus_tag="PLW82_03155"
     CDS             568..1413
                     /locus_tag="PLW82_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ46304.1"
                     /translation="MKIVLHSYTFRGYSVERAFYKAAEYGYDGVELSVVHFKDEADIE
                     KAVALSKKYQVPIVALDGGTDLLNEGNADASIERMKWLIDMAAKYNVPMINGGIASKY
                     GPDPGNYGANGSVLLSDADYEYMAEKYQEVAKYAEANQILIVFEIHMNTPHDTGAATL
                     KLIEMINSDWVKANPDPGNIFATSSSEGPYAAVAKVIDHVAFFHAKNCRRTALGNGYD
                     YSWLLENGAIDYYRIVCQLVEYGYDGYICIEYCGEGDPAIAAEKDIVYLRRLIKDALL
                     SNCLK"
     gene            1410..1847
                     /locus_tag="PLW82_03160"
     CDS             1410..1847
                     /locus_tag="PLW82_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46305.1"
                     /translation="MRKVVLVLLVICCFGGWALAEEAGFSNLIYGVVQGTKDLKAVYS
                     QPVRVDYKKSALAFLGTFTASLLDKSVFAASSSLNFLDPVAEIITKTVEPIGLGIMYT
                     AFGWKNKELARKIVGSSLFTAINTVSLKHLIGKARPYASSRPL"
     gene            complement(2391..2466)
                     /locus_tag="PLW82_03165"
     tRNA            complement(2391..2466)
                     /locus_tag="PLW82_03165"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2431..2433),aa:Ala,seq:cgc)
     gene            complement(2535..3239)
                     /locus_tag="PLW82_03170"
     CDS             complement(2535..3239)
                     /locus_tag="PLW82_03170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOJ46306.1"
                     /translation="MSKKSLPSWLMEAPLLVLFVLVLLPLALKSLGVFGLSQENIIIL
                     AMVLQQVGFLLGTFILKKRHPVHPTDILDTRFEWKAFWRSLLWVPAIVLINVLGIVLS
                     TQLFTPLLGADRLQSILLQETSRVNIFLLSSWRLVVATIMVCVVGPISEELFFRGFFL
                     SRAVHTYSQTVGYLLTSLFFVLPHLYLINSIPIFLLSLVLCYIALNENLWAAVGAHVF
                     FNTVVFLNVLLRAGVG"
     gene            complement(3433..>4385)
                     /locus_tag="PLW82_03175"
     CDS             complement(3433..>4385)
                     /locus_tag="PLW82_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOJ46307.1"
                     /translation="EEAKAILQEHRIEKLPLVDEKFHLKGLITIKDIEKARKYPNSAK
                     DGQGRLLAAAAVGVGSDLDERAGLLVDAGVDILVVDTAHGHSIGVIEAVKKLKRTYPH
                     VDLIAGNVATGEATKALIEAGADAVKVGIGPGSICTTRVIAGIGVPQITAVNDAYVVA
                     KEYGVPIIADGGIKYSGDVAKAIAAGADTVMIGNLFAGCEESPGETEIYQGRSFKVYR
                     GMGSLGAMKKGSKDRYFQEDEKKLVPEGIEGRVPYKGPVSETIFQLLGGLRAGMGYCG
                     CKDIGEMKEKTQFMRITAAGLRENHPHDVHITKEAPNYSI"
BASE COUNT         1177 a         1095 c         1039 g         1074 t
ORIGIN      
        1 ccccgttggt aaagaccact tcatctcctg tgtccaagtc cacggcattg atgatcgtgg
       61 tcatttgtaa atcgctgaaa cgttttccgc cagttcgctt tgcgatctcc tctgcgatgt
      121 agtcaccttt gaagagtccg tcgggcgcct tgattttggg attgaagcct gcaaaatccc
      181 caatgatttt ggcgagtacc aaaggatact gccacgcttt tatggcctta tcaaagactt
      241 ttttagggtc aaggcttaaa gcgaaggctt ccatctgttc tcctgtaagc cccgaagcat
      301 atagcgccgc aacgatggaa cctgcagatg taccggcaat agcacttggc ctgagaccgt
      361 atcggtccag taccttaatg gcaccaatgt gggctgctcc ccgaagggaa ccgccgctaa
      421 gagacagccc ccaacgcata tctatccctc ccactattgc atatgaacag gggagcttga
      481 ccaagaacaa aacatcacct gttttagcag gatatccctg ctccgacacg aagaagtgta
      541 gaggaaaatt gctgtgaggt gaactgaatg aaaatcgttt tacattccta tactttccgt
      601 ggttattcgg tagaaagggc tttctataaa gctgctgagt acggctacga cggcgttgag
      661 ctatcggttg tacacttcaa agatgaagcg gacatcgaaa aagctgttgc gctttccaag
      721 aagtatcagg tgcccatcgt agctctcgac ggaggtacag accttctcaa cgagggcaac
      781 gccgatgctt ccatcgaaag aatgaaatgg cttatcgaca tggccgcaaa atacaacgtg
      841 cccatgatca acggcggtat cgcttccaag tacggacccg atccaggcaa ctacggtgca
      901 aatggctctg tgcttctctc tgatgccgac tacgagtaca tggcagagaa gtatcaagaa
      961 gtcgccaagt acgcggaagc caaccagatc ctcatcgtct ttgagattca catgaatact
     1021 ccacacgaca ccggcgctgc aacgctcaaa cttattgaga tgatcaactc cgattgggtc
     1081 aaagccaatc ccgatccggg caacatcttt gctacaagca gcagcgaagg tccatacgct
     1141 gctgtggcaa aagtcatcga tcacgttgcg ttcttccacg ccaaaaactg cagaagaacc
     1201 gctctgggca acggctatga ctacagctgg cttttggaaa acggcgccat cgactactac
     1261 cgcatcgtct gtcagctagt cgagtacggt tacgacggct acatctgcat cgagtactgc
     1321 ggcgaaggcg accctgcaat tgcagctgag aaggatatcg tctacctcag aagactgatt
     1381 aaggacgctt tactttccaa ctgcctgaaa tgagaaaagt agttcttgtt ttgcttgtta
     1441 tctgctgttt cggcggatgg gcgttggctg aagaggctgg gttttcaaac ttgatctacg
     1501 gggttgtcca aggaacaaaa gacttgaaag cagtctacag tcagcctgtg cgcgtagact
     1561 acaaaaaaag cgccctggct ttccttggca cttttaccgc aagtctcctt gacaagtcgg
     1621 tctttgctgc ttcgtcatct ttaaatttct tagaccctgt agcggagatc atcaccaaaa
     1681 ccgtagagcc cataggcctc ggaatcatgt acactgcatt tggctggaag aacaaagaac
     1741 ttgcccgcaa aatcgttggt tcatcgcttt ttacggcaat caacacggta agtttaaagc
     1801 atttgatcgg gaaagcccga ccatatgcca gcagccggcc tttgtaaatc gggcccaatc
     1861 tgaaaacaga ctacaactct tttccgtccg gccatacctc tttaagcttc agtttggcaa
     1921 ctgtcttgag cagcgaatac cctgattatt ccgtcttgtt ttacagctgg tcttcctgcg
     1981 taggactgag ccggattctc ttggagcgcc actggattgg agatgttgta ggaggagcaa
     2041 ttgcaggaac tgcttcagca agattttatc tggaatctgt acgcgaggag ccgtaaatcc
     2101 tgcgaaagcg tcgaaaaaca ataaacaaga caaaagaagg actgcactaa gtcggccttg
     2161 ttgctgacat gtgcagtcct ttgtttgcga acgtaaaagc actaacacaa aaccttttac
     2221 taacaacttc tgtactttaa gaaacccttc ttaatgggca ttgcaccagg aacagtatcg
     2281 agcaaatggg caagttttac tctacctgag tgcagtttgc tcaaagcaca gaatcagtgc
     2341 aaaaaaaatc tatcctagcg gatagatcga tgctttagca actgcgtaaa tggtggaggt
     2401 gagcgggatc gaaccgccga cctttagctt gcgaagctaa cgctctccca actgagctac
     2461 acccccgtcg aggaaattat accaccgaga aaaaagccgt tcaagaaaga aaaaacacct
     2521 gctttgagat tccttcaacc tactcccgca cgcagaagta cgttgaggaa aaccactgta
     2581 ttaaagaaaa catgcgcacc aacagctgcc cacaggttct cgttcagagc aatgtagcag
     2641 agaacaaggc tgagaaggaa aataggaatg gagttgatca ggtacaagtg gggcagcaca
     2701 aagaacaggg aagtcaaaag ataccctaca gtttgcgaat aggtgtgtac agccctagac
     2761 aggaaaaatc ccctgaaaaa cagttcctcg ctgatgggac caacaacgca gaccataatg
     2821 gtagcaacta ccaaccgcca agagctaaga aggaagatgt tgactcttga agtttcctgg
     2881 agcagaatcg actgcagcct gtcagctccg agcagcggag tgaacagttg cgtagaaaga
     2941 acgattccca atacattgat aagaacaatc gcaggaaccc acaaaagact cctccaaaaa
     3001 gccttccatt cgaacctggt atctaagatg tctgtggggt gaacagggtg tcttttctta
     3061 aggataaacg ttcccaagag aaatccaacc tgctggagaa ccatggcgag aatgatgatg
     3121 ttttcttgag aaagcccgaa cacgccaagg gactttaagg caagcgggag caagaccagg
     3181 acaaaaagca ccaaaagcgg agcttccata agccagctgg gcaatgattt ttttgacaca
     3241 aaaaacacgc taagagaacc gtggctcttg gcgttttcac ctcctgtatc ttgtagtcag
     3301 gtgccatggc attaactaga ttatttaatc taaaggcctt agattccttc agtggagtga
     3361 aaacgatttt actgtgtcac acgtaatcga acttcaaaac agcaaaaaag ggctgcggag
     3421 cagccctttt tgttaaatgc tgtagtttgg tgcttctttg gtaatgtgca catcgtgcgg
     3481 atgattttct ctgagacccg ctgctgtaat gcgcataaac tgagtcttct ctttcatttc
     3541 gccaatgtct ttgcagccgc agtagcccat accggcccgg agacctccga gcagctggaa
     3601 aatggtctcg gagacgggac ctttgtaggg aactctgcct tcgatgcctt ctggaacgag
     3661 cttcttctcg tcttcttgga agtagcggtc cttgctgccc ttcttcatag cgcctagaga
     3721 gcccatacct ctgtagactt tgaagctacg gccttgatag atctctgtct ctcctgggct
     3781 ttcctcgcaa cctgcaaaga ggttgcctat catgacggtg tctgcacctg ccgcgatggc
     3841 tttggcaaca tcaccggaat acttgatgcc gccatctgca ataatgggaa ctccgtactc
     3901 tttggccacc acgtaggcgt cgtttacagc tgtaatttga ggcacgccaa tgccggcgat
     3961 gactctggtg gtgcagatag aaccgggacc aatgccaact ttaactgcat ctgctcctgc
     4021 ttcaatcagc gccttcgtgg cttcgccggt tgcaacgtta cctgcgataa gatcgacgtg
     4081 aggataagtc cgtttcagtt tctttacagc ctcgattacg ccaatggagt gtccgtgagc
     4141 agtgtcgact acaaggatgt cgactcctgc atctacaagg agacctgccc tttcatctag
     4201 atcggagcct acgccaactg ctgcagctgc taggagcctt ccctgaccgt ctttagctga
     4261 gttggggtac tttcttgctt tctcaatgtc cttgatggta atcagacctt tgagatgaaa
     4321 cttttcatct acaagaggca gcttctcgat tctgtgctcc tgcaaaatgg ctttggcttc
     4381 ctcaa
//
LOCUS       DAOSDA010000095         4365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12644_length_4365_cov_3.951508, whole genome shotgun sequence.
ACCESSION   DAOSDA010000095 DAOSDA010000000
VERSION     DAOSDA010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12644_length_4365_cov_3.951508"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /gene="rpsD"
                     /locus_tag="PLW82_03180"
     CDS             <1..545
                     /gene="rpsD"
                     /locus_tag="PLW82_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOJ46308.1"
                     /translation="YSDKCAFDRRPYAPGQHGQSRKKLSEYALHLREKQKARRIYGVL
                     EAQFVRYFKAAEAKKGVTGDILMQTLERRLDNVVYRLGFARSRAEARQIVRHGHIAVN
                     GKKNDIPSYLVNEGEVIAIRAERRDAEVFAGLQESLTNHKTPAWLELDVENLQGKVLH
                     LPERSEIDVDVQEQLIVELY"
     gene            641..1582
                     /locus_tag="PLW82_03185"
     CDS             641..1582
                     /locus_tag="PLW82_03185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOJ46309.1"
                     /translation="MIEIEKPKIEIQEKTSTYGKFVVEPLERGYGTTLGNSLRRILLS
                     SLPGSAVTSIKIDGVLHEFSTIPGVVEDVTDIVLNMKKLLVVSTSDEPVIGRIHKEGP
                     GEVTAADIVIGPEVEILNPDLVIATLEEEGKLSLELRVERGRGYVSAERNKRDDMPIG
                     EIAIDSIFTPVKKVNFTVENTRVGQVTDFDRLILETWTNGSIEPDGAVSLAAKIMSEH
                     LRLFIDLTDATSDIEVMVEKEEKEKDKVLEMSIEELDLSVRSFNCLKRAGINTVEELI
                     KKTEEDMMKVRNLGKKSLEEVKSKLAEYGLSLRSEDE"
     gene            1611..1955
                     /gene="rplQ"
                     /locus_tag="PLW82_03190"
     CDS             1611..1955
                     /gene="rplQ"
                     /locus_tag="PLW82_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOJ46310.1"
                     /translation="MKRGTGRLGRDAAERRALLRSLSTAFFVAEKIETTEAKAKVLRP
                     VVEKLITLGKNDTLSARRRAASYLLDEVAVEKLFTEIAPRYKDRPGGYTRIVKTGVRR
                     GDATPLAIIELV"
     gene            2019..2840
                     /locus_tag="PLW82_03195"
     CDS             2019..2840
                     /locus_tag="PLW82_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOJ46311.1"
                     /translation="MEPIIEVKDVYHQYGETEALSGVSLEIFPGDFVAVVGANGSGKS
                     TLARHLNGLLLPSKGTVRVKNFYTHNPEDIWSVRALVGMVFQNPDNQLVATTVEEDVA
                     FGPENLGVPSNEIRARVDEALKWVNMLDHKKRSPHQLSGGQKQRVAIAGVLAMHPEVL
                     VLDEPTAMLDPVGREEVLQVINHLNKELQMAIVLITHHMEEVAMAKRVIAMAEGKIKV
                     ECNPIELFLQTELVDELGLDVPQVFALGNLLRKYGLEVDPAFDPEELVNALCRSN"
     gene            2825..3685
                     /locus_tag="PLW82_03200"
     CDS             2825..3685
                     /locus_tag="PLW82_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOJ46312.1"
                     /translation="MQIELRDVSYTYNGPIPVAALHNINLKVKTGEILGLIGHTGSGK
                     STLLQHLNGLLKPQDGDVLVDGKSVKRGEVSLPKLRFRVGLVFQYPEYQLFEETIFKE
                     VAFGPKSMGLNEDEIKQRVAKSLSDVGMSEEFYERSPFELSGGQKRRVALASILAMEP
                     EILVLDEPTAGLDPKGRKEILDFLLKWRTDDKGIVLVSHNMEEMAPFADRIVVLHQGK
                     VVLDGTPKEVFSHTQELLDVGIKVPQVSMVVNSLRSRGYNIDAGIFTVEQAAEAIFDA
                     LRGGGERRGL"
     gene            3675..>4365
                     /locus_tag="PLW82_03205"
     CDS             3675..>4365
                     /locus_tag="PLW82_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HOJ46313.1"
                     /translation="MAFDITLGQYIPGDSFVHNLDPRTKLIIAIALIVVLFLVNTFVG
                     YFWIALFLLLATINGSISARSVVRSIKPLTFILLLTVILNILFTEGDILFQLGFIRIT
                     EQGVFRGLLLGLRLILLILSTTIMTLTTSPLAITDALENLLGPLKKIKFPVHELSMML
                     TIALRFIPTLLEETDKIMKAQMARGADFESGNLLKRAQNMVPLLVPLLVSALRRADDL
                     AAHLEGSDAEPG"
BASE COUNT         1163 a         1009 c         1139 g         1054 t
ORIGIN      
        1 gttacagcga caagtgcgcc ttcgatcgtc gtccatacgc accaggacag cacggacaat
       61 cccgtaagaa gctgtctgag tacgcccttc acttgagaga aaagcagaaa gctcgccgca
      121 tctacggagt tttagaagct cagtttgtgc gctacttcaa agccgctgag gccaagaaag
      181 gtgtcacagg tgacatcttg atgcagaccc tagagcgccg tttagataat gttgtatatc
      241 gtttgggctt tgctcgttcc agagctgagg ccagacagat cgtacgccac ggtcacattg
      301 ctgtgaatgg taagaaaaac gacatacctt cctacctagt caatgaagga gaggttatcg
      361 ctatccgggc tgaacgtaga gacgctgaag tctttgccgg attacaggaa tctctgacca
      421 atcacaaaac gccagcgtgg ctcgaactcg atgttgaaaa cctgcagggt aaagttctgc
      481 acctgccaga acgctctgaa atcgatgttg atgttcaaga gcaactcatc gttgagctgt
      541 attaatacgc tgcatagact tagaagtgct gtttgattaa tacgctcgtc gttgtctttg
      601 ctcggaggct agcgctgctt gaataaggag ggtgtggcga ttgattgaaa tagaaaagcc
      661 aaagatcgag attcaggaga aaacgagcac ctatggcaag tttgtcgtag aaccgcttga
      721 gcgtggctac ggaaccacac tgggaaactc gctcagacgg atcctgctct catcccttcc
      781 agggtcagcg gtgacttcca ttaagataga cggagtcctg cacgagttct ctacaattcc
      841 cggagttgtc gaagatgtta ccgatatcgt gctgaacatg aaaaagctac tagtcgtctc
      901 tacttccgat gaacctgtaa ttggtagaat tcacaaagaa ggacccggag aggtcaccgc
      961 agctgacatt gtgatcggac ctgaagttga aatcctcaat cccgatctgg tgatcgcaac
     1021 tttagaagaa gaagggaaac tgtcgcttga actgcgcgtt gagcgcggac gcggttatgt
     1081 ttctgccgaa agaaataaaa gggacgacat gccaatcggt gagattgcca tcgactcgat
     1141 ctttactccc gtgaagaagg taaacttcac tgtagagaat actcgtgtcg gccaggtaac
     1201 agactttgac agactgattc tagagacttg gactaatggt agcatcgaac ccgacggagc
     1261 tgtaagtctt gcagcaaaga tcatgtccga gcacctgcgt cttttcatcg accttacaga
     1321 cgcgacttca gacattgagg ttatggtcga gaaggaagag aaagaaaaag ataaagtgct
     1381 ggagatgagc atcgaagaac tagatctttc tgtccgttcg ttcaactgct tgaagcgtgc
     1441 aggcatcaat actgtagaag aactgatcaa gaagactgaa gaagatatga tgaaagtccg
     1501 caacctcggt aagaaatcgc tggaggaagt taagtccaag ctagccgaat acggcttgtc
     1561 tctgcgttcc gaggacgaat aatttctggt tgaagaggga ggtgctgccc atgaaacgtg
     1621 gtactggacg actcgggcgt gatgctgctg agcgcagagc actgctccgt tcgctatcga
     1681 ccgccttctt tgtggctgaa aagatagaaa ccacagaggc caaggccaaa gtcttgcgcc
     1741 ctgttgtgga aaagctaatc actcttggta aaaacgatac gctgagtgcg cgtcgcaggg
     1801 ctgccagcta cttgcttgat gaagtagcag tggaaaagct gttcactgaa atagccccaa
     1861 gatacaagga ccgcccaggc ggatacacta ggattgtaaa gactggagtt cgccgtggcg
     1921 atgcaactcc acttgcgatc atagaattag tctaaatttc agggcgcgga gacctaatag
     1981 tgggttctct gcgcttgtgc taggtggggg gatagtttat ggaacccatt atcgaagtaa
     2041 aagacgtata ccaccagtac ggagagaccg aggctctgag cggtgtatcc ctcgagattt
     2101 ttccgggaga ctttgtcgct gttgtaggtg caaacggttc tggtaagtcc acactagcaa
     2161 ggcacctcaa cgggcttttg ctcccatcca agggcaccgt cagagtcaaa aacttctata
     2221 ctcacaatcc cgaggatatc tggagtgttc gagctttagt tggcatggtg tttcaaaacc
     2281 ccgacaacca actggtagcc acaactgttg aagaagacgt ggccttcgga cctgagaacc
     2341 tcggcgttcc cagcaacgag atccgtgcta gagtagatga agcgctgaaa tgggtaaata
     2401 tgctggacca caagaagagg tctcctcacc aactgtccgg aggacagaaa cagagagttg
     2461 caattgcagg agtcctggct atgcatcctg aggttttagt tttggacgaa ccaacggcaa
     2521 tgctggaccc tgtaggccgt gaagaagttt tgcaggttat taatcattta aacaaagaac
     2581 tgcagatggc aatcgttttg attacccatc atatggaaga agttgccatg gcaaagcgag
     2641 tgatcgccat ggcagaaggc aaaatcaagg ttgaatgcaa tcctattgaa ctcttccttc
     2701 agaccgagct agtagacgag ctagggctag atgtgcctca ggtttttgca ctgggcaatc
     2761 ttctgcgaaa atacgggtta gaagtcgatc cggcatttga ccccgaagag ttggtgaacg
     2821 ctctatgcag atcgaactaa gagatgtctc ctacacctac aacggtccca tacctgtggc
     2881 tgctctgcat aacatcaatc tcaaggttaa aaccggagag attctcggac tgatcggtca
     2941 cactggctcg gggaagtcca cgctattgca gcatttaaat ggccttttaa aaccacaaga
     3001 tggggacgtt cttgttgatg gaaagtcggt aaagcggggc gaggtatctc tgccaaaact
     3061 gcgttttcgc gtgggactgg tatttcaata tcccgaatac cagctctttg aagagaccat
     3121 ctttaaagag gtcgcgtttg ggcctaaaag catgggacta aacgaagacg aaattaagca
     3181 gagagtcgcc aagtctttaa gcgatgtggg catgagcgaa gagttttacg agagatcacc
     3241 tttcgagctg tcaggcgggc agaagcgaag agttgccctt gccagcattc tggcgatgga
     3301 acctgagatt ctggttctcg acgagcctac agccggcctt gacccaaagg gccgaaagga
     3361 gattctggac ttcttgctta agtggagaac cgacgacaag ggaattgtct tggtctccca
     3421 caatatggaa gagatggctc cctttgcaga ccgcatcgtt gtactgcatc aagggaaagt
     3481 ggttctagat ggcactccta aagaggtgtt ttcccacacc caggagctgc tcgatgtggg
     3541 catcaaggtt cctcaggttt cgatggttgt aaatagtctt agaagcagag gttacaatat
     3601 tgatgcaggc atcttcacag tagaacaagc cgcagaagcc atctttgacg ctctgagagg
     3661 cggaggtgag cgtcgtggcc tttgatatta cgctaggtca gtatatacct ggagattcat
     3721 ttgtacacaa tctcgaccct aggacgaagc tgatcattgc tatagcgctg atcgttgtgc
     3781 ttttcctggt caataccttt gtgggctact tctggattgc gctgttcctt ctgcttgcta
     3841 cgatcaacgg cagcatttct gcaagaagcg ttgtaagaag cattaagccc ctcacattta
     3901 ttctactgct tacggtaatt ctcaatatct tgtttaccga gggcgacatt ctctttcaac
     3961 tggggtttat ccgcataacg gagcagggag tgtttagagg gctgctcctt ggcctcaggc
     4021 tgatcttact tattctaagc actacgatta tgacacttac tacctcacct ctggctataa
     4081 ccgacgccct tgagaacctt ctcggaccgt tgaaaaagat taaatttccc gttcacgagc
     4141 tgagcatgat gcttacgatc gcactccgtt ttattcccac actcctcgaa gaaaccgaca
     4201 agatcatgaa ggctcagatg gcaagaggcg ccgactttga gtccggcaac ctgctgaaac
     4261 gggctcagaa catggttccg ctccttgtac ctcttcttgt cagcgctctc agacgggcag
     4321 acgatctggc tgcgcacctc gaaggttccg atgcggaacc aggcc
//
LOCUS       DAOSDA010000096         4351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12687_length_4351_cov_3.775372, whole genome shotgun sequence.
ACCESSION   DAOSDA010000096 DAOSDA010000000
VERSION     DAOSDA010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12687_length_4351_cov_3.775372"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLW82_03210"
     CDS             complement(<1..555)
                     /locus_tag="PLW82_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46314.1"
                     /translation="MKLLEVRNLKKTYTTRFGGSQVQALRNVSFTVDSGEFIAIMGES
                     GSGKTTLLNIIAALDRPTSGEVLLNGRNIHSIKESEVAAFRRDNLGFVFQDFNLLDNF
                     SIQDNIFLPLVLARKPYKEMHERLQPIAHMLGISHILSKFPYEVSGGEKQRAAVARAL
                     ITNPKIILADEPTGALDSRASDNLL"
     gene            complement(665..1663)
                     /locus_tag="PLW82_03215"
     CDS             complement(665..1663)
                     /locus_tag="PLW82_03215"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOJ46315.1"
                     /translation="MEVFLSYFKRQLGRVAFGLIVTGIFFLTLYLYGVPLEPALYAAV
                     LAIAFGVVCYGYGFVRFARRHRRVLSARKQLAVQLPEFPQPRDIIEKDYQEMIEALFS
                     DLTKKITEVENERSELLEYYTLWVHQIKTPISAMKLLLEEEDSELNTELSLELFKIER
                     YVEMVLNYLRIESPSSDFVFKKHDLAELVKQAIRKYARMFVKKKIRLNLQEFQCEVLT
                     DEKWLVFVLEQVISNAIKYTDEGTVSIYLASSERKELMIEDTGIGIAAEDLPRVFDKG
                     YTGYAGRLDKRATGIGLYLSKKILTKLSHTIDIESTPGKGTRVKIGLETVSPEVRD"
     gene            complement(1665..2336)
                     /locus_tag="PLW82_03220"
     CDS             complement(1665..2336)
                     /locus_tag="PLW82_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ46316.1"
                     /translation="MYRILIVEDDAVIASAMKKGLEVWGYEVECVSDFKNVLSDFARI
                     DPQLVLLDISLPFYNGYHWCGEIRKFSKVPIMFISSASDNMNIVMAMNMGGDDFIAKP
                     FDLNVLTAKVQALLRRTYDFAGTSSLLEHRGAILNVSDGTFTYDGEKVELSRNELKIL
                     QVLMENSGKVVSRDTLMVKLWETDSFVDENTLSVNVNRLRRKLESLGLTDFITTRKGL
                     GYLVE"
     gene            complement(2473..4170)
                     /gene="argS"
                     /locus_tag="PLW82_03225"
     CDS             complement(2473..4170)
                     /gene="argS"
                     /locus_tag="PLW82_03225"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOJ46317.1"
                     /translation="MGIAKEIVAESIHAALPELDLREIADLIEVPPSQDLGDYSFPCF
                     SLAKRFRKSPVQIASDIAGKIPENKLFDRVNVVNGFVNFTLNQSELASMLLPKVIKEH
                     RDYGKNSEGVGKTVVIDFSSPNIAKPFSVAHLRSTVIGHSLARIWEHMGYNVEKVNHL
                     GDWGTQFGKVIVAYRRWGDEPSQHLKGSIRHLLELYVRFHKEAEVEPTLDDEARLWFK
                     KLEDGDPEAYALWEEFRKTSLDNLQEVYELMGITFDHYTGESFYNDLIPATIEKLEPM
                     MEVSEGADIVPLGDDMPPFLVRKSDGATLYGTRDVTAAIYRHETFNFAKCLYVVGDEQ
                     SLHFKQVFTVLKKLGYDWADNCEHVPFGLMRFGDMKLSTRQGNTIFLEDVLNKAIELT
                     KSIIEEKNPNLEDKDAVARMVGVGAVIFNDLRNNRIKTINFEWDDLLNFQGDTGPYVQ
                     YTHARASSLLARAGEEINLNEVNLSLLKEEEEKVLITQLERFPDVVVLAARSNEPSTV
                     ARYVLGLARDFNKFYQVHRILDEDTDLKKARLTLVDAVRKTIDTALYLLGLESPEKM"
BASE COUNT         1077 a         1089 c          953 g         1232 t
ORIGIN      
        1 caacaggtta tcgctggctc tagagtccaa agctcctgtc ggctcgtcgg ctagaatgat
       61 cttgggattt gtaataagcg cccgtgccac tgcggctctc tgcttttccc ctccggaaac
      121 ttcgtaagga aacttactca aaatatgcga gatgccgagc atgtgggcta tgggctgcag
      181 ccgctcgtgc atttctttgt aaggttttcg agccaaaacc aacggtagaa agatgttgtc
      241 ctgaattgag aagttgtcga gtaggttaaa atcctggaag acaaatccca aattgtctct
      301 cctaaaggca gcaacttcgc tttcttttat ggaatgaatg tttctgccgt ttagaagaac
      361 ctcaccgctt gtgggcctgt caagagcagc gatgatgttc aaaagtgtag tttttccgga
      421 accggattct cccatgatag cgataaactc tccggagtct actgtgaacg agacattgcg
      481 tagggcttga acctggctcc ctccaaatct cgttgtgtaa gtctttttca ggttccttac
      541 ctcaagtagc ttcataacca tttcccccta agtcgatttc aacctgagta taacaaaagg
      601 ggaaaacgcg aagccataac gctggcttac attctcccgc cgtatcttac aaatccgtaa
      661 gaatctagtc gcgcacctct ggggacactg tctcgaggcc aatcttcacc ctggtcccct
      721 tcccaggtgt ggattcgata tcgatggtgt gcgatagctt ggtcaggatc tttttcgaaa
      781 gatacaaacc aatacccgtt gcccgtttgt cgagtctccc agcataacct gtgtagcctt
      841 tatcgaaaac ccgagggaga tcctcagcgg caatgccaat tcccgtatct tctatcatta
      901 gttccttgcg ttcggagctt gccaaataaa tggagacggt gccctcatcg gtgtatttga
      961 tggcgttgga aataacctgc tccagtacga acaccagcca cttctcgtcg gtgagaacct
     1021 cgcattgaaa ctcctggaga ttaagtctga tctttttctt cacgaacatc cttgcgtact
     1081 tgcgtattgc ctgcttcacc agctctgcaa ggtcgtgctt cttgaagacg aagtccgaag
     1141 acggactttc tatgcggagg tagttcagaa ccatctccac atacctttca atcttgaaga
     1201 gttctaagct gagctccgtg ttgagttcac tgtcttcttc ttcgagaagg agcttcatgg
     1261 cagagatcgg agtctttatc tgatgtaccc acagagtgta gtactccaga agctccgatc
     1321 gctcgttttc tacctcagtt atctttttcg tgaggtctga aaacagcgct tcaatcatct
     1381 cctgatagtc tttttcaatg atgtctcttg gctgcggaaa ctccggtagc tgcactgcca
     1441 gctgctttct tgcagacaaa acccgtctgt gccgcctggc aaaacgcaca aacccataac
     1501 cgtagcagac aacgccaaag gcaatcgcaa gtactgctgc atacaaagcc ggttccaaag
     1561 gtacgccata caggtaaagg gtgagaaaga agatgccagt cactataaga ccgaaagcaa
     1621 cacggcctaa ttgtctcttg aagtatgaca aaaagacttc catcttattc caccagataa
     1681 cctaagccct ttctggtcgt gataaaatcc gtcaaaccta aagactccag tttccgcctt
     1741 aggcgattca cattgacact tagagtgttt tcgtcgacga agctgtctgt ctcccaaagc
     1801 tttaccatca gcgtatctcg gctcactacc ttcccactgt tttccataag cacctgtaag
     1861 atcttcagct cgttgcggct tagttccact ttttccccgt cgtaggtgaa ggttccgtcg
     1921 ctgacgttga gtattgctcc gcggtgctcc aagagactgc ttgtacctgc aaaatcatac
     1981 gttctcctaa gcaatgcctg tacttttgct gtcaaaacat ttaggtcaaa gggctttgcg
     2041 atgaaatcat ctccgcccat gttcatggcc ataactatgt tcatgttgtc cgacgctgat
     2101 gagataaaca tgataggtac tttagagaac ttacggatct cgccgcacca gtggtagccg
     2161 ttgtaaaacg gcagcgatat gtccaaaagc actagttgag ggtcgatgcg cgcaaaatct
     2221 gagagcacgt ttttaaaatc gcttacgcac tcaacctcgt atccccaaac ctccaaaccc
     2281 tttttcatgg ctgaggcaat tacagcatcg tcttcaacaa ttagaattct gtacatctca
     2341 tcacctccga cgaaactcag tttccagaac ctctagcgga gaggctgttt gctaactact
     2401 ttttgcttaa agaagcataa atcctgtttg cattcagaag taaaaaaccc ggcataagcc
     2461 gggctctttt atttacatct tttctgggga ttcaagtcct aggagataga gtgccgtatc
     2521 gatggttttc cgtacagcat ctaccagcgt tagtctggct ttcttgagat ctgtgtcctc
     2581 atcgagaatg cggtgaactt ggtagaactt gttgaagtct cttgcaagac caagaacata
     2641 ccttgcaact gtagaaggct cgttagatct tgcggcaagt acaaccacat ctgggaagcg
     2701 ctctagctgt gttatgagaa ccttttcctc ttcttctttc agaagcgaga ggttgacttc
     2761 gttcagattg atctcctcac cggctcttgc caaaaggctg ctggctctgg catgagtgta
     2821 ctgaacgtac ggaccggtgt ctccctggaa gttcagaaga tcatcccact cgaagttaat
     2881 tgttttaata cggttgttcc tgaggtcgtt gaagataacc gctccaacac cgaccattct
     2941 agcaactgca tctttgtctt cgagattggg gttcttttct tcaataatag atttggtcag
     3001 ttcaatagct ttattcaaga catcttccaa gaagatggtg ttgccctgtc ttgtggatag
     3061 cttcatgtct ccaaatctca tgagcccaaa aggaacgtgc tcgcagttgt ctgcccagtc
     3121 gtatccgagt ttcttcagaa cggtgaaaac ctgcttgaag tggagactct gctcgtcgcc
     3181 gaccacatac aaacacttag cgaagttaaa tgtctcgtga cgataaatgg ctgctgtaac
     3241 gtctcttgtg ccgtacagag ttgcaccgtc tgatttgcgc accaaaaatg ggggcatatc
     3301 gtctcctagc ggcacgatat ccgcaccttc actaacttcc atcatgggtt cgagcttctc
     3361 gatagttgca gggatcaagt cattgtagaa gctttcccct gtgtagtgat cgaaggtgat
     3421 gcccatcagt tcatagactt cctgaaggtt atccaagctg gttttcctaa actcttccca
     3481 aagcgcatag gcttcgggat caccgtcttc aagcttttta aaccacagcc tggcctcatc
     3541 gtcgagagta ggttcaacct ctgcttcttt gtggaagcgg acgtagagct cgaggagatg
     3601 cctgatagag cccttaagat gctggctagg ctcgtctccc catcttctgt aggctacgat
     3661 tactttaccg aactgggttc cccagtcacc aagatggttg accttctcaa cattgtagcc
     3721 catgtgttcc cagattctgg ccaaagaatg accgattact gtggacctca ggtgggcaac
     3781 gctgaacggc tttgcgatgt tcggcgacga aaagtcgatt acaactgttt tgcctacacc
     3841 ctcgctgttt ttgccgtagt ctctgtgctc tttaatcact tttggcaaga gcatcgaagc
     3901 gagctcgctt tgattcaaag tgaagttgac aaagccattg accacattaa ctcgatcaaa
     3961 aagtttattt tcagggatct tcccggctat gtcgcttgcg atctgcacag gagatttcct
     4021 gaaacgcttc gccaagctga agcacggaaa agagtagtca cctagatctt ggctaggcgg
     4081 aacctctatt agatcggcta tttcccgcag atctagttct ggcagtgcgg cgtgaataga
     4141 ctccgcgaca atttcttttg caatacccat aattacgctc cttaacgcaa actattagag
     4201 gcttaagtat attatttatc cttctaatta atataacact ttacccctga tctctcaacc
     4261 gtttcctccg gcaaatgtgg ttatcttgtg atagtgtcaa gttgagtccg tataattgtg
     4321 taaaattggt ttccccatga aataaaagaa g
//
LOCUS       DAOSDA010000097         4348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12707_length_4348_cov_3.338924, whole genome shotgun sequence.
ACCESSION   DAOSDA010000097 DAOSDA010000000
VERSION     DAOSDA010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12707_length_4348_cov_3.338924"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLW82_03230"
     CDS             complement(<1..322)
                     /locus_tag="PLW82_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ46318.1"
                     /translation="MAEQKAKILVVGSFMMDLVVRTPRVPENGETIIGTSFNRFPGGK
                     GANQAVAAARLGGAVTMAGKVGSDDFGDEFLSVLASEGINTDYILRDSQAATGVGFIT
                     LDHAG"
     gene            complement(325..933)
                     /locus_tag="PLW82_03235"
     CDS             complement(325..933)
                     /locus_tag="PLW82_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017471423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ46319.1"
                     /translation="MRVVELVKQVVQTEAEALSKLVDQIDESFAEAIEIIQKCQGKVV
                     VTGVGKSGIVGKKIAASLSSTGTPAFFVHACEAVHGDSGMIEERDVVVMISNSGETRE
                     VLDVVPVLDQIGCKRIAITSNHESTLAKLSDVSLAYNYEKEADHLNLAPTTSTTLTMV
                     IGDALAVALSILKEFSEEDFHRYHPGGSLGAKLSDLQNKGDR"
     gene            complement(1393..2028)
                     /locus_tag="PLW82_03240"
     CDS             complement(1393..2028)
                     /locus_tag="PLW82_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46320.1"
                     /translation="MLKAATMLFLIIILAFSSFFIGAMATFLLLEHKDENTLKSIAVT
                     PGGVSGYLKFKMTYIYFMSVIGIIVILVGTKLIAGDKYVIMGYSLFDRIELSHMIAFA
                     LVNGLFTPALGLLQAAVAKNKVEGFAMIKGTGILALVPALMVLQSFQGRLQYLLGVFP
                     NFWALRGMYVEFMPFEGTANLSFPLYLLIGALYNILVLILAYRLYLKKAEY"
     gene            complement(2191..2583)
                     /locus_tag="PLW82_03245"
     CDS             complement(2191..2583)
                     /locus_tag="PLW82_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46321.1"
                     /translation="MALFVIHRITFNYAALLAVVLIAGAAHAAIGFVMSLASKDFTTM
                     LGFLMAYMFVFTIPSVLYSLGVIDAKYEWLLMLSPSHSANHLLTSAVKGEFNLTMTVF
                     GSVYLVLLTVGLFRFVVYPQFKSNAVRG"
     gene            complement(2823..3665)
                     /locus_tag="PLW82_03250"
                     /pseudo
     CDS             complement(2823..3665)
                     /locus_tag="PLW82_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3741..>4348)
                     /locus_tag="PLW82_03255"
     CDS             complement(3741..>4348)
                     /locus_tag="PLW82_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ46322.1"
                     /translation="ESNPLPKFPNLLDAALGEFCAKKYEEASLNEILKKSGMSKGSFY
                     HHFGDKYGLYLAIIDLIVKKKVSYFFPVLQEKSDESGGFFNALRDVMKSTMNFMLPVD
                     ERLQHLFSRLMEESTEFRERLYEFFPYYDYSRAFYEYIQKAIASGEIDDRYSPEFVVG
                     IIEIVFSNLYKLVSSSDPKEILDAAIKAVDMMERGIGGKKG"
BASE COUNT         1216 a         1117 c          861 g         1154 t
ORIGIN      
        1 tgcctgcgtg atcaagggta atgaagccca cacctgttgc tgcttgactg tcgcgtaaaa
       61 tgtaatcagt gtttatacct tcagaagcga gaacagacaa gaactcatca ccaaagtcat
      121 cagaacccac tttgcctgcc attgtaactg ctccacctaa gcgagcagca gcaaccgctt
      181 ggtttgcccc tttgcctcca ggaaaacggt tgaaactggt accgattatc gtttctccat
      241 tttctggaac ccgtggagtc ctaaccacaa gatccatcat gaaacttccc accaccagga
      301 ttttcgcttt ctgctctgcc atactcatcg atctcctttg ttctgcagat cggacaactt
      361 tgcacctaaa ctgccccctg gatgatagcg atgaaagtct tcctcgctaa actcctttaa
      421 tatggataaa gcaacagcta atgcatcgcc gatcaccatc gtcaaagtgg tgctagttgt
      481 cggggccaga ttcagatgat cggcctcttt ttcatagttg taagccaggg aaacgtcgct
      541 tagttttgca agagtggact catggttaga agtaatcgca atccgtttgc acccaatctg
      601 atccaatacc ggtactacat ccaacacttc tctcgtctca ccgctgttag atatcataac
      661 aaccacgtct cgttcctcaa tcatacctga atcgccatga acagcttcac atgcatgcac
      721 aaagaaagcc ggagtccctg tgctagacag agaagcggca atcttcttgc ccacaattcc
      781 ggatttgccg acgccagtta ctacaacctt accttggcac ttctgaataa tctctatagc
      841 ttcggcaaaa ctctcatcga tttgatccac cagcttgctg agtgcttcag cttcagtttg
      901 gactacctgc ttgaccaact ccactacacg catgctattc caccttcctc accattatcc
      961 gactgctact tgccataaaa gtgctcgctg accttaaacc taaaaatccc gaacgtctta
     1021 ttaagccagt actcttaaca gaacaatacc gtctttgaca tgcaatatca aggtttcatt
     1081 ttccacgttc gctttaagaa tacgccttca agcaggcagt tcctttgcta acgtagttag
     1141 tgttggtatg actcttactc cggtcacaag tctctcttaa aaacgcatca atgaccgtag
     1201 ttttcatgta catggctgtg cgtatcgttc tcgtgtccaa acaacgaaca ggatagaacg
     1261 aatgaaatgt taaaagcgct gcccaaactg gacagcgctc tgttgtattg tagaaaccgg
     1321 gaagactccg tcgacgtaaa cgcagtaact gtgtggtctg tttatctcaa tcaccttacg
     1381 tctgtaggag ttttagtatt ctgctttttt caaatacagt ctgtaagcca gtattaaaac
     1441 caggatgttg tacagagccc cgataaggag atacagcgga aaacttaagt ttgccgttcc
     1501 ttcaaaaggc ataaactcca catacatacc tctgagcgcc cagaagttcg ggaacacacc
     1561 caaaagatac tgaaggcgtc cctggaaaga ctgaagaacc atcaaagccg gaaccaaggc
     1621 caagataccg gtgcccttaa tcatggcaaa gccctccacc ttgttcttgg caacagccgc
     1681 ttgcagtaga cctaatgctg gagtaaaaag cccgttgact aacgcaaaag caatcatgtg
     1741 cgacaattct atcctgtcga aaagggagta ccccataatc acgtatttgt ccccagcaat
     1801 gagcttagtg cccaccagaa ttacaataat gccaataacc gacatgaaat aaatgtaggt
     1861 cattttgaac ttcagatacc ctgaaactcc acctggtgtc actgcgatgc tttttaacgt
     1921 gttctcgtct ttgtgctcca aaagcaagaa cgtagccatg gcgccaataa aaaacgaact
     1981 gaatgccaaa atgataatca gaaagagcat cgtggcggct ttgagcataa cctcctgcat
     2041 caaatccaac gatccaaaga ttttaggaaa cacatatcct gacaaaacta agataatgag
     2101 cgggaacaag cacatataga agttcagagg gtcgcgaaaa atggtttttg tttagtactt
     2161 taaaagtccg aggagttttt tcatgtcaga ctaccccctt acggcattgc tcttgaactg
     2221 gggatacaca acgaatctga aaagccctac ggtaagtagt accagatata cgctaccaaa
     2281 aacagtcata gttagattga actcgccttt cactgctgaa gtcaaaaggt ggttggcact
     2341 gtgcgaaggc gacagcatca acagccattc gtacttagcg tcgatcactc ccaagctata
     2401 aagcacagaa ggtatggtaa aaacgaacat gtaagccata aggaaaccga gcatggtggt
     2461 aaagtctttg cttgcaagag acattacaaa accgatggcg gcatgagcgg cacctgcgat
     2521 gagcacaact gcaagcaaag ctgcgtaatt gaaggttatt ctatgaatca caaaaagtgc
     2581 cacagatact ataagtgccg actctagtgc cagcaccaga gatgctatgg ttttagctac
     2641 aagaactgtc catttttatc tgccgaaatc tctccatttt attatgccac tcacacgtgc
     2701 ttcagctcca gagacaaaca aaaacaatat gacccaaagc acggaggttg caaggcttac
     2761 aggaaggatc ttgtaccgta tgagcctctt cacttcgcct acagttaact tgatcaggct
     2821 gttcacttca gagcaactcc tgtcacgaca atgaagatct cttccatgga tgtctcgcca
     2881 ctgtgtatgg tttcgatggt cttggtcttt aagagtttgg caaagtcttc gtcgtcgcca
     2941 attccgtcga gagggaaagt tgcgctggct aattcgccgt tttccctgta ctctacctta
     3001 acttcccgtt tgccgtactt cagcttaaga tctctaggag ttgatacttc cacaagctta
     3061 ccatttacac agaatccaat ccggtcgcag agctgctcta catcgttcat caagtgtgta
     3121 gtgagaaaca ccgttcctcc gctgtcacgg tattctgcga ttatttcctt gacaatcata
     3181 gcgtttttag gatcgagacc gtttgtcacc tcgtccaaga atagcactct cggtttgttc
     3241 agcatagcgc gaacgaagtt caaacgcacc ttcattcctt tagaatactc ccctaccttc
     3301 atgtcgcggt attaccagat gccaactctt tccatcagcg ctttgatgtc tgccgtcttc
     3361 ttgtaaaatc ctgaaaagaa cttcaagttt tccattgctg ttaacttggt gaaatgcacg
     3421 gggacttcaa aacctacgcc gatgtcttca taaaactcgg ggcccaagct tttaaggtct
     3481 ttcccgaagt attcaatgct accctggtag tcttcaagaa gcctgattaa aatcttttgc
     3541 gtcgtagact tacctgcacc tgaaggccca agcaaaccga agatctcacc ttcacggacc
     3601 tcaaaactga ttcctttaat cgtgtcttct tttgcagtgg gatacctgaa cgtcaagtcc
     3661 tccaccttaa acgcaactcc caatgtatgt ccccctctca cgaaaataaa gagcaacttc
     3721 agctcttgct gttaaaaact ttatcccttt ttcccgccaa tgccgcgctc catcatatcg
     3781 actgctttaa tggctgcatc aagaatctct ttgggatcgc tgctcgaaac cagcttatag
     3841 agattggaaa acacgatctc aatgatcccc accacaaact caggagaata cctgtcatcg
     3901 atttctccag atgcgattgc tttttggatg tattcgtaaa aagcccgact gtagtcgtag
     3961 tacgggaaaa actcatagag gcgttctcga aattccgtac tttcctccat caacctgctg
     4021 aacagatgct gtaagcgttc atctactggc agcatgaaat tcatggtgct cttcatgacg
     4081 tccctgaggg cgttaaaaaa tcctccgctc tcgtctgact tctcctgaag aactgggaaa
     4141 aagtacgaga ccttcttttt gacgatgagg tcgataatcg ccaaatacaa gccgtatttg
     4201 tcaccgaagt ggtgataaaa gctacctttg ctcatcccgg acttcttcag aatctcattg
     4261 agggatgctt cctcgtactt cttagcacaa aactcaccaa gggcagcatc aaggaggttt
     4321 ggaaacttcg gcaacgggtt gctttccc
//
LOCUS       DAOSDA010000098         4332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12768_length_4332_cov_3.410101, whole genome shotgun sequence.
ACCESSION   DAOSDA010000098 DAOSDA010000000
VERSION     DAOSDA010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12768_length_4332_cov_3.410101"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /gene="pgk"
                     /locus_tag="PLW82_03260"
     CDS             complement(<1..138)
                     /gene="pgk"
                     /locus_tag="PLW82_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOJ46323.1"
                     /translation="MQKKTIKDIDVKGKRVLVRVDFNFPTDDSGNITDDRRIRSAVPT
                     IQ"
     gene            complement(200..1207)
                     /gene="gap"
                     /locus_tag="PLW82_03265"
     CDS             complement(200..1207)
                     /gene="gap"
                     /locus_tag="PLW82_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOJ46324.1"
                     /translation="MVKVGINGFGRIGRLVFRHALNDPNIEIVAINDLTDAKTLAHLL
                     KYDSMYGILDADVSATDDSIIVNGKEIKITAERDPANIKWGEAGVEVVVEATGIFRSK
                     EKVQPHFDNGAKKVIITAPAKGQDITIVMGVNEDKYDPEKHHVISNASCTTNCLAPVA
                     KVLDDNFGIIRGFMTTVHSYTNDQRILDLPHSDLRRARAAALSIIPTTTGAAKAVAEV
                     LPHLKGKLDGFAMRVPTPTVSIVDLVAEVNADVTKEEVNAVLKKAAEGELKGILAYTE
                     EPLVSVDFRGNPNSSIVDSELTNVMKDETGKTLVKVVAWYDNESGYALRVVDLVKYIS
                     K"
     gene            complement(1298..2335)
                     /locus_tag="PLW82_03270"
     CDS             complement(1298..2335)
                     /locus_tag="PLW82_03270"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOJ46325.1"
                     /translation="METSIEKQIFELLRTVLPDLEERLDERYLVLRAVNFLQPIGRRT
                     LSDYLSTGERIVRSIVEVLKEQHLVEVTPLGIVTTEKGKDTLWKLKEYIEKIKGIEKL
                     EKQIAGKYQLQKAVVVAGDADKDLGVKQEMCTAAAEILLEVLKPGNILAVSGGTTMAM
                     VADSMDALTKSIDITVVPARGGLGEDVEKQANTVAAKLAKALGGSYRLLHVPDVLEEE
                     LLKPLLSSTPISDVIDLIKRADVLLHGIGTAEEMAQRRDLHPELWQSIVDKGAVGEAF
                     GYYFDSSGRIVHTTPSAGLRLEDLANIKVCIAVGGGMSKAEAIEAFLKVASVDIVVTD
                     EAVARELAQDN"
     gene            complement(2442..3548)
                     /gene="whiA"
                     /locus_tag="PLW82_03275"
     CDS             complement(2442..3548)
                     /gene="whiA"
                     /locus_tag="PLW82_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOJ46326.1"
                     /translation="MRLSPEYSFPECCLRGNGVLLKPNKISSYSLPRAGFFSALENLN
                     SRKDVVEISELNFSQRVRNELARVKPTHPSCKWAELFGLIQTIGTLNISTRGPSLEIK
                     TENAAVARKAVTLFKELGQMDSELIVEKETKLKKRNLYRIRVSAEDFSRIFPESIHSN
                     ILDKDVPTFAEDQECCRRSYLRGLFLGSGSVQNPEKTYHLEIRIAEKRFAERLKEFLD
                     GTDLKFGVVERRNEFVLYIKDSDSVATFLTMVGAHSSLLHLENIRIIKDMKNNINRIV
                     NCEDANIEKTITASVKQVQAIDLLEERGMLKTLPKSFQEVARVRKENFDASYTKIAEI
                     LEISKSAVSYRLKKIEDMAEKIAKDRRKDQEDEE"
     gene            complement(3469..4011)
                     /locus_tag="PLW82_03280"
     CDS             complement(3469..4011)
                     /locus_tag="PLW82_03280"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ46327.1"
                     /translation="MNLKKLATMSVLVALSIVFTLLIRIPFPPAPFLIYEPGDVPILV
                     GGLCFGPVAALAMTVTASVLMGFFHPEGGVFGVVMHIIATGLLTVIPAMIYKGDVKSA
                     RKGLILGALAMSGIMPVLNVILNPIFYGMPRASVVQLLLPGIIPFNLIKASLNAAIAG
                     VLVPRVLPSWKRSFVEAKQD"
     regulatory      complement(4097..4209)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         1048 a         1086 c          950 g         1248 t
ORIGIN      
        1 ctgaatggta gggactgcag agcggatgcg gcggtcatcg gtgatgtttc cgctgtcgtc
       61 tgtaggaaag ttgaaatcca ctcttaccag aactcgtttg ccttttacgt cgatatcttt
      121 aatagtcttt ttttgcatag ccattacctc cgaaaagggg cccatgagat gcgttctcta
      181 agggccccaa ttgttaaact tacttagaga tgtacttaac caagtcaact actctaagtg
      241 cataaccgga ctcgttgtcg taccaggcaa cgactttgac aagtgttttg cctgtttcgt
      301 ccttcataac gttggtgagc tcgctatcta cgatggaaga gtttggattg cctctaaagt
      361 caacggatac cagaggctct tcggtgtagg ccaaaatgcc cttgagttcg ccttcagctg
      421 ctttctttag cactgcgttg acttcttctt tggtcacgtc ggcgttcact tcagctacca
      481 agtccacgat ggaaactgta ggtgttggaa cgcgcatagc gaacccgtcg agcttacctt
      541 tgaggtgagg cagtacttca gcaacagctt tagctgcacc tgtagttgtc gggataatgc
      601 tcaatgctgc tgcgcgagca cggcggagat cggagtgagg taggtcgaga attctctggt
      661 cattggtgta agagtgtaca gtggtcatga agccacggat aatcccgaag ttgtcgtcga
      721 ggactttggc aactggagcc agacagttgg ttgtgcagga tgcgttggag attacatgat
      781 gcttctcggg atcgtacttg tcttcgttta cgcccataac gatggtgatg tcctgacctt
      841 tagcaggagc tgtgatgata actttcttag caccgttgtc gaagtgaggt tgaactttct
      901 ctttgcttct gaagatacct gtagcttcga cgacgacttc gactcctgct tcaccccatt
      961 tgatgttggc tgggtctctt tcagcagtga tcttaatctc tttaccgttt acgataatgg
     1021 aatcgtctgt agcgcttaca tcggcatcaa gaatgccgta cattgagtcg tatttgagca
     1081 gatgagcgag ggtttttgca tcggtaagat cattgatagc aacaatttcg atatttggat
     1141 cgtttaaagc atgtctgaac actaggcggc cgatacgacc aaaaccatta atacctactt
     1201 taaccatagt tggctgaaac cgcttggagt ttcagcgttc acctcctaaa ttgagtattt
     1261 attggtatcc catatctcat cgatacaggt agaaccctta attgtcttga gcaagttccc
     1321 ttgctacagc ttcatcggta accacaatat caacagatgc tactttgaga aaagcttcaa
     1381 tggcttcggc tttactcata cctccaccaa cggcgatgca aaccttgatg ttggcaaggt
     1441 cctctagcct tagccccgcc gacggagtcg tgtgaacaat cctgccagag ctgtcgaaat
     1501 agtaaccaaa agcttctcca actgctcctt tgtccacaat gctctgccac agctcggggt
     1561 gcaaatctcg cctttgagcc atttcctctg cagtgccaat accgtgaaga agcacatcgg
     1621 ccctcttaat caagtcgatg acatcactga taggagtgct tgaaagaagc ggttttaata
     1681 gctcctcttc cagtacatcg ggcacgtgca aaagacgata gcttccgccc aaggcctttg
     1741 ccagcttcgc agccactgtg tttgcctgct tctcaacgtc ttcaccgaga ccgcctctag
     1801 cgggcaccac ggtgatatcg atggatttag taagggcatc cattgaatcg gcaaccattg
     1861 ccatggtggt gccaccggaa acagcaagga tatttcccgg tttgaggacc tctagaagta
     1921 tctctgcagc agcggtgcac atctcctgtt tgactccgag gtctttgtct gcatctccag
     1981 ctacgaccac tgctttttgc agctggtact ttccggcgat ctgcttttct agcttttcta
     2041 tacccttaat cttctcgatg tattctttga gcttccataa agtatctttg cctttttccg
     2101 tagtgacaat gcccaggggc gtcacttcga cgagatgctg ttctttcagc acctctacga
     2161 tacttcggac gattctttcg cctgtggaaa ggtaatcgga aagtgtcctc cgcccaatag
     2221 gttggaggaa gttcaccgct ctcaagacga gataacgttc atcgagcctc tcctcaaggt
     2281 cgggaaggac agttcgcagc agttcaaata tctgcttttc aatacttgtt tccactacta
     2341 attcacacct atgtctgcag ggactttttt tgtcccgtat tttaattttc tgtcccacca
     2401 gcctctgaaa gaaaccggca taatcaccgg tctcatatat actattcttc gtcctcctga
     2461 tctttccttc tatctttggc aattttttca gccatatctt cgattttttt caacctgtag
     2521 cttacagcag atttgctgat ctccaagatc tctgcaatct tagtgtagct tgcgtcaaag
     2581 ttttcttttc taaccctggc aacttcctga aaacttttcg gcagggtctt cagcatgccg
     2641 cgttcttcca agaggtcgat ggcctgcacc tgttttaccg atgcggttat ggtcttctcg
     2701 atgttggcat cttcgcagtt gactatccgg ttgatgttgt ttttcatgtc tttgatgatt
     2761 ctaatgtttt ctaaatgcag cagagaacta tgtgccccaa ccattgtcag aaacgttgcc
     2821 acagaatcgc tatccttgat gtacaagacg aactcgtttc gcctttcgac aactccgaac
     2881 ttcaaatctg tcccatcaag aaactctttc agtcgctcag caaacctttt ctcggctata
     2941 cgaatctcaa ggtgataagt tttttctgga ttttgtacag atccgctgcc caaaaacaat
     3001 cccctcaagt aagatctgcg gcagcattcc tgatcctcag caaaagtggg tacatctttg
     3061 tccagtatgt tgctatgtat agactcagga aatatccggc taaaatcttc agcactcacc
     3121 ctgatccggt agaggttcct cttcttcagt ttagtttctt tttcaacgat caactccgaa
     3181 tccatctgtc ccaactcttt gaacagagtg accgctttcc ttgctacagc tgcgttttcg
     3241 gtcttgatct ccagggaggg tccccgcgta ctgatgttca acgttccaat ggtctggatt
     3301 aatccgaaaa gctctgccca tttgcatgac ggatgggtag gtttaacacg cgccaactca
     3361 tttcttactc tttgggaaaa attcaactcg cttatctcta ccacatcctt tctactgttt
     3421 aaattctcca gggcagaaaa aaagcctgca cggggcaggc tgtaggaact aatcttgttt
     3481 ggcttcaaca aaactccgtt tccacgaagg cagcactcgg ggaacgagta ctccggcgat
     3541 agccgcatta agagaagcct taatcaagtt aaacggaatt atccctggaa ggagcagttg
     3601 taccactgac gctctcggca tcccgtaaaa aatcggattc aaaataacgt ttaacacggg
     3661 catgatcccg ctcatggcca gcgcaccgag tataaggcct ttgcgagcac tcttcacgtc
     3721 ccctttgtag atcatcgccg gtatcaccgt aaggagaccg gtagcgatga tgtgcattac
     3781 cacaccgaaa acgccacctt ctggatggaa gaaacccatc agtaccgatg cagtcaccgt
     3841 catagctaag gcggcgacag gtccaaagca cagaccccct accaaaatcg gtacatcacc
     3901 tggttcgtag atcaaaaacg gtgcaggtgg gaacggaatt ctgatgagca gagtaaaaac
     3961 aatgctcagt gcaacgagaa cggacatggt agccagcttt tttaggttca taaaaaagac
     4021 ctccttgcaa tcctgccgtc aaggagatcc ttcagggtaa aaaagcaaag agaatagctg
     4081 cttcagctag accctatcga tctttctccc atccggacta taaccgtcgg ttctggagtt
     4141 tcaccagatc tgcttgcgct cgtggactat accaccggtt aggaatttca cctgaccctg
     4201 aaagatcaac tgcggtttgt tttttcttta actatattat acccaacaag taaaaaagtg
     4261 caaccactat tcgcaacaag atcgcgttca agttttgtag ggatcgatgt agttccttgt
     4321 tgtgctgtga tt
//
LOCUS       DAOSDA010000099         4328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12785_length_4328_cov_3.484203, whole genome shotgun sequence.
ACCESSION   DAOSDA010000099 DAOSDA010000000
VERSION     DAOSDA010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12785_length_4328_cov_3.484203"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1643)
                     /locus_tag="PLW82_03285"
     CDS             complement(45..1643)
                     /locus_tag="PLW82_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOJ46328.1"
                     /translation="MMRSLYSGVTGLRTHQTKMDVIGNNIANVNTVGFKGSRVTFQEI
                     LTQTIRGATAPQDDKGGINPMQVGLGVGLGSIETVHKPGNLQGTSNASDLAIEGDGFF
                     VVSDGNKNYYTRAGAFSLDDDNNLVSSNTGMTVMGWMAVNGKIDLNKPLERIQIVKGD
                     TIEPVATSNINYSGNLNANTTGKLELKANPWVVSSGAHSARLHMALEPTGDFNEFRFV
                     ISPVSGRITTVSGPQANITGVIELNSDGSIKSIQMDSTETFTISASDDPASVIGDLVI
                     DPSDPSSLFAVGGEKLALSDFLFTPADPAVTALQIIDSQGNEHEITMKFTKLANNQWR
                     WEVDSPNLETLGAAYGEITFTSGGTVQNVTYFDASGNVASSLRFRVPGDADVLVIQAA
                     FDKLTQFAQQTDLRRDHNGYKAGTLNDFLVDTRGVITGIYSNGLNRAIGQIAVANFAN
                     PAGLLRAPGTLFEESSNSGAAQIGAPGTGGRGSVKPSTLEMSNVDLSEEFTEMIITQR
                     GFQANSRIITASDEMLQEIVNLRR"
     gene            complement(1715..2092)
                     /locus_tag="PLW82_03290"
     CDS             complement(1715..2092)
                     /locus_tag="PLW82_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HOJ46329.1"
                     /translation="MTPIIKPKITPHLSNVQPTSTQSTKRPPQGDFASHLEKALGEEV
                     RFSKHALQRIQSRELTPSDTQIKRLNEGVMLAKEKGSQNTLVLVDDLAFVVSVKNNTV
                     VTALSGRETARKVFTQIDSTVIN"
     gene            complement(2102..2509)
                     /gene="flgD"
                     /locus_tag="PLW82_03295"
     CDS             complement(2102..2509)
                     /gene="flgD"
                     /locus_tag="PLW82_03295"
                     /inference="COORDINATES: protein motif:HMM:NF007197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HOJ46330.1"
                     /translation="MRIESQSSISLQKSETDRNILGKDDFLKLLITQLRYQDPTNPVD
                     DKEFIAQMAQFSSLEQMQNLNKLVQEYVGTQQAMTMFAQATNLIGLQVKVDKGDGTVD
                     EGTVEAVRFSTAGPVIMVNGKDYLITDLIEISK"
     gene            complement(2524..4104)
                     /locus_tag="PLW82_03300"
     CDS             complement(2524..4104)
                     /locus_tag="PLW82_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOJ46331.1"
                     /translation="MDVGIVSLEAALSLPKEPAKSRSEPLGFGAFLKAAIQGKKRSSG
                     KPEPEVQEQQESELVGESEPVAAFWNLQFAPEIRPQAGEVQEGESVEPLGLWEQDLKS
                     TDLSLEQLTAAVPVPADGGQEGNFLELYGMEVVGEALETEIELTELPLEVPLEAPPAT
                     ALEAASDAVASEDQGVSQVGVSIIEDLPAGTYNLGILEGETSELTAVDLLKETYPRQT
                     YGSTLEAEPKAEVEVASLHTRAAVSSRSAKEASTTEVQLVSPLDRKAVLQAYGKLSNL
                     QKSLEASPEISKLPEEGTSLEEEAEVSAVRGATSEAWDESIGTSDGKDLVAAEKSRFE
                     VRRTGDVQKEKQPLNLEFEVSFAEALKELGARELSGSTPSRVQGDRALEVLEYDQFFE
                     VLAGRLTSASEEEQSVNIRLYPKELGELKISVSVKADVVQLKLETETVLSQRIIEEQL
                     PVLKKALESQGLQLGEFSLGTNFFNHQGSYETRLELASEFSKLMSLNGRSEEGIPSEN
                     PPEVAYTKPKDGLVDYRV"
     gene            complement(4109..>4328)
                     /locus_tag="PLW82_03305"
     CDS             complement(4109..>4328)
                     /locus_tag="PLW82_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46332.1"
                     /translation="KANVESLRFEAMLLSEMETRSVLAILENLPEDRKVDLLASMDQD
                     TVIAILQSMDPQEAAALLPKVSSKMEGR"
BASE COUNT          908 a         1160 c         1042 g         1218 t
ORIGIN      
        1 ctgtgagata tgaatggtat ttaaatgggg gaagccacta gatactatct acgcagattg
       61 actatctctt ggagcatttc atcgcttgcg gtaataatgc gggagttggc ttggaacccg
      121 cgctgagtaa tgatcatctc cgtgaactct tctgacagat ccacgttgga catctccagt
      181 gtggatggct tgaccgagcc tcttccgcca gtgccaggcg caccaatttg ggctgcgccg
      241 gagttagaag actcttcgaa cagagtacca ggtgccctga ggagaccggc tgggttggca
      301 aagtttgcca ctgcgatctg tccaatggct ctgtttagac cgttggagta gatacctgtg
      361 ataacacctc ttgtgtccac caagaagtcg ttcagtgtac cggctttgta accgttgtga
      421 tcacgcctta ggtctgtctg ctgggcaaac tgggtcaatt tatcgaaggc agcttgaatg
      481 acaagcacat cggcatctcc cggcactcta aagcgcaggg aagacgcgac attaccgctt
      541 gcgtcgaaat aggttacgtt ctgcactgta cctccagaag tgaaggtgat ttcaccgtat
      601 gcggcaccta gagtctccag gttcggtgag tctacctccc aacgccactg gttgttggcg
      661 agcttggtaa acttcatcgt gatttcgtgc tcattgccct gggaatcgat aatctgcagt
      721 gcagtaacag ctggatctgc gggagtgaag aggaaatccg aaagggcgag tttttctcct
      781 cccacagcaa acagggagct gggatctgac ggatcgataa ctagatcgcc gatcaccgat
      841 gccggatcgt cgcttgcgct gatggtgaaa gtctcagttg aatccatctg tatcgatttt
      901 atagaaccat cgctgttcag ttcgatcaca ccggtgatgt tggcctgcgg tccgctaaca
      961 gtggtaatcc tgccggacac cggagaaatc acaaagcgga actcgttgaa gtctcctgtc
     1021 ggctccaagg ccatgtgcaa tctagctgaa tgggcaccgc tggaaaccac ccacggattg
     1081 gctttgagtt ccaacttgcc cgtagtgttg gcgttgagat tgccggagta gttaatgttg
     1141 gaggtggcaa ccggctcgat ggtatctcct ttaacgatct ggattctctc cagaggttta
     1201 ttgagatcaa tcttgccgtt gacagccatc cagcccatga cggtcattcc cgtgtttgaa
     1261 gaaaccaggt tgttgtcgtc gtccaagctg aacgcacccg ctctggtgta gtagtttttg
     1321 ttaccgtcgg acaccacgaa aaacccgtcg ccttcaatgg ccaggtccga ggcattgctc
     1381 gttccttgaa ggtttcctgg tttatggacg gtttcaatgg atccaagacc gacaccaagg
     1441 cctacctgca ttgggttaat gccgcctttg tcgtcctgag gtgcagttgc gcctctgatg
     1501 gtctgggtga ggatttcctg gaaagttacc cgtgaacctt taaaaccgac tgtattgaca
     1561 ttggcaatgt tattgccaat aacgtccatt ttggtctgat gtgttctcag gccggtaacg
     1621 cctgagtaaa gcgaacgcat catagtcata ccctccattc atgccgcttc cgtcgatccg
     1681 cggcataaag cgtcctttgc agggcccgct ttttttaatt tattaccgta ctgtcaatct
     1741 gcgtaaaaac tttcctagct gtctctcttc ccgatagggc agtgacaacc gtgttgttct
     1801 tcacgcttac tacaaacgcc aagtcgtcga cgagcaccag agtattctgg ctgcccttct
     1861 cttttgccag cattacccct tcattcagcc tcttgatctg cgtatcgctg ggagttagct
     1921 ccctgctctg aattctctgt agagcgtgtt tggaaaatct aacttcttca cctagtgctt
     1981 tctcgaggtg gctcgcaaag tcaccttgag gaggtcgttt agtgctttgg gtactagttg
     2041 gctgaacatt ggacaggtga ggcgtgatct tgggtttaat tatcggagtc atgcatctca
     2101 cctacttgct tatttcaatc agatcggtta ttagataatc tttgccgttt accataatca
     2161 ccggtcctgc tgtactgaac cgaacggctt caactgtgcc ttcgtcaacg gtaccgtcgc
     2221 ccttgtccac cttcacctga agaccgataa ggttggttgc ttgagcaaac atcgtcatcg
     2281 cctgctgcgt gcctacgtac tcctgaacca atttattcag gttctgcatc tgctccaagg
     2341 agctgaactg cgccatctgt gcgatgaact ctttgtcgtc tacaggattg gttggatcct
     2401 ggtaccttag ctgagtgata agcagtttaa gaaagtcgtc tttgccaagg atgttcctgt
     2461 cagtctcgct tttctgcaga gaaatactgc tctggctttc aattcgcact taaatcacct
     2521 gccttatacg cggtagtcta ccaaaccgtc tttaggtttg gtgtaggcta cctccggtgg
     2581 attttctgaa ggtatgcctt cttcagatct cccattcaag ctcatcagct tggaaaactc
     2641 gctggctagt tccagccttg tttcgtagct tccctgatgg ttgaaaaagt tggttcccag
     2701 actgaactcg ccaagctgga gaccttgact ctccaaagct ttctttaaga ccggaagctg
     2761 ctcttcgatg atcctctggc tgagaacggt ttcggtctca agcttcagct gcacaacgtc
     2821 tgccttcacg ctaacggaga tcttcagctc acccaactct tttgggtaga gtctgatgtt
     2881 taccgactgc tcttcttcag aagcgctggt cagcctccct gccaggacct caaagaactg
     2941 gtcgtactcc aaaacctcca gggctctgtc tccctgcacc ctacttggtg tgctccccga
     3001 aagctcccta gcaccgagct ctttcagcgc ttcggcaaag ctcacttcga actcaaggtt
     3061 tagcggctgt ttttctttct gcacatcccc ggtccttcga acctcgaaac ggctcttttc
     3121 cgctgccacg agatcttttc catcggaagt tccgatggac tcatcccaag cttcagatgt
     3181 ggccccacgc accgcggaaa cctccgcttc ttcttccaaa ctcgttcctt cttctggcag
     3241 tttgctgatt tctggtgatg cttctaggga cttttggaga ttggaaagct tgccgtaggc
     3301 ctgcagcacc gctttgcggt ctagagggct cactagctga acctctgtcg ttgaagcttc
     3361 ttttgcgctt ctggagctta ccgccgctct tgtgtgaaga gaagccactt caacctcagc
     3421 cttcggctct gcttctagag tgctgccgta ggtttgtctg gggtatgtct ctttaagcag
     3481 atctacagca gtaagttcag aggtttcgcc ttccaagatt ccgaggttat aggtgcctgc
     3541 aggtaggtct tcgatgatgc tgaccccaac ctgagaaaca ccttggtctt cacttgcaac
     3601 tgcatcagaa gctgcttcta gagcggtagc tggcggtgct tcaagcggca cttcaagggg
     3661 aagttcggta agctcaattt ctgtttccaa tgcttctccc actacttcca tcccgtagag
     3721 ctcgaggaag tttccctctt gcccaccatc tgctggcaca ggcactgctg cagttagctg
     3781 ttccaaagag agatctgtgc ttttaaggtc ctgctcccaa agacctagag gctcaacaga
     3841 ttcaccttct tgaacctcac cagcttgagg cctaatctct ggtgcaaact gcagattcca
     3901 aaaggcagct acaggttctg attcgccgac aagctcagac tcctgctgct cttgcacttc
     3961 gggctctggc ttgccgcttg aacgcttctt gccctgtatt gccgctttga gaaaggcacc
     4021 aaaccccaga ggctcgcttc tggactttgc aggctcttta ggcaggctta acgcagcctc
     4081 cagacttaca attcctacat ccatccattc acctcccttc cattttgctg gatactttgg
     4141 gaagcagcgc tgctgcttct tgtggatcca tactctgcag aatggcaatc accgtatcct
     4201 ggtccatact ggctaataga tccacctttc tatcttcggg aaggttctcg aggattgcga
     4261 gaaccgatct ggtttccatt tcactgagga gcatagcttc aaatctgagg ctctccacat
     4321 ttgccttg
//
LOCUS       DAOSDA010000100         4297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12912_length_4297_cov_2.880952, whole genome shotgun sequence.
ACCESSION   DAOSDA010000100 DAOSDA010000000
VERSION     DAOSDA010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12912_length_4297_cov_2.880952"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLW82_03310"
     CDS             complement(<1..367)
                     /locus_tag="PLW82_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ46333.1"
                     /translation="MEIRKVETDFANSYIISDTDPEGRKVAVVIDPALELEELLGLDV
                     VLCLATHGHADHINNAKKICDHFEIPLTLHEKEAIYVKDDSYNLSRYVLHRPLDKPEN
                     LKLLSGGDELSFLGHTIKVY"
     gene            complement(373..816)
                     /gene="dtd"
                     /locus_tag="PLW82_03315"
     CDS             complement(373..816)
                     /gene="dtd"
                     /locus_tag="PLW82_03315"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOJ46334.1"
                     /translation="MLAHIQRVKEAKVVVNGETVSEIKAGLLVLLGVVVGDEPHDADY
                     LVDKIVNLRVFSNGDKEFDLSVKDIGGEILIVSQFTLAADCRKGRRPSFSDAMNPVEA
                     EKLYEYAADALKVHVPVKTGVFRAHMDVSLVNDGPVTILLDSRKG"
     gene            complement(832..2979)
                     /locus_tag="PLW82_03320"
     CDS             complement(832..2979)
                     /locus_tag="PLW82_03320"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOJ46335.1"
                     /translation="MEIGDLLQRMRQYASEDSLKTVEKAYEFAKKAHEGQFRESGAPY
                     IEHPLEVAYILADLELDVDTIIAGLLHDVVEDTAIREETIKAEFGQQVALLVDGVTKL
                     EKLPTLSKEEQQAENIRKMFLAMAKDLRVVLIKLADRLNNMRTLRHLSPERQKKNAEE
                     TLDIYAPLAHRLGMWRIKWELEDLAFRYLYRDEYYYIVEKVAKNRKEREVFINDVIEK
                     LQERLLELGIKAEIYGRPKHLYSIYMKMVKQGKEFEEIYDLTAVRVIVDTVRDCYAVL
                     GTVHTLWKPMPGRFKDYIAMPKSNMYQSLHTTVIGDNGEPFEVQIRTWEMHRTAEYGI
                     AAHWKYKEGSKGHEVNEKFTWLRQILEWQRETRDVEEFMETLKIDLFEDEVFVFTPKG
                     DVVTLPTGATPVDFAYEVHTQVGNTCVGCKVNGKIVPLDYQLKTGEFVEILTSKHSSP
                     SQDWLNFVKTSKARNRIRQKLRELQREDSIVKGKDLLQRECRRMEWEPGSILLTDRLE
                     ELARDYNLTSVDDLYAAIGYGKLISADVLKELVGEKEYNARKRDLRTKHLLNTTQKVV
                     KPGGAVSVKGTDNMLIKLARCCSPVPGDEIVGYITRGRGVTVHRKDCPNVNDLEPERL
                     IEVVWDETDTGYYPVELHLEGYDRPNLLTSIMTTLNDNKTTTAAVNARALDDGRASIQ
                     LTVNIESIADLENVMNRLRQIRGIVSVTRARPT"
     gene            complement(3044..3556)
                     /locus_tag="PLW82_03325"
     CDS             complement(3044..3556)
                     /locus_tag="PLW82_03325"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOJ46336.1"
                     /translation="MDFASKIRTIENFPKEGVSFKDITTLFQDPEAFKAMIDTIKDRY
                     KDEKIDTIVGIESRGFLVGAPLAYALGAGFVLVRKPGKLPCKVERVEYELEYGTDALE
                     IHSDALKPGERVLICDDLLATGGTTKATIELVKKLGAEVVSLAFIIELTYLNGREKLE
                     GYDIYSLVKY"
     assembly_gap    3597..3606
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3627..>4297)
                     /locus_tag="PLW82_03330"
     CDS             complement(3627..>4297)
                     /locus_tag="PLW82_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46337.1"
                     /translation="DLPSCGIIPAFEVLHKKLLLDPRITLVRTTRDLNMPETKPQLVP
                     EKVGFEIDDDHKLMAFIASGAKECTLSYLPGDYWLWRLFWEVAQGCLRVGIGFTAEEL
                     TKVQRAHSLRYPSDDDLRRIYIYLKSTAYRSVWRESFRQAAIDLQNRYDKPFSEESVE
                     TAVQIFLELGLMTKTKINGTGRTGYVLEDAKQKLDLNASLSYNEGIIRKQELDRYARE
                     VAAL"
BASE COUNT          959 a         1135 c          988 g         1205 t
ORIGIN      
        1 gatagacttt gatagtatga cctaaaaatg agagctcatc gccgccggac agaagtttca
       61 gattctccgg cttgtccagg ggtctgtgaa gaacatatcg actgaggttg tagctatcat
      121 ccttcacgta aatcgcttct ttttcgtgaa gggtaagagg tatctcgaag tggtcgcaaa
      181 tcttcttagc gttatttata tgatcagcgt ggccgtgagt tgccaagcac aaaaccacgt
      241 cgagtccgag gagctcttct agttcaaggg caggatcgat tacgactgcc acttttctcc
      301 cctctggatc tgtatcgctg ataatatagg agttggcaaa atcagtctca actttgcgaa
      361 tttccatctt ttctaccctt tcctgctgtc tagaagtatg gttacaggac cgtcgttgac
      421 caggctcaca tccatgtgag ctctgaaaac tccggtcttt acaggaacgt ggacctttaa
      481 tgcatctgct gcatattcgt agagtttctc agcctccact ggattcatgg catcggaaaa
      541 actcggcctt ctgccctttc tgcagtcggc agccagggta aactggctga caataaggat
      601 ctccccacct atatccttta cagaaaggtc gaattccttg tctccattgg agaaaaccct
      661 gaggttaaca atcttgtcta ccaggtagtc cgcatcatga ggctcgtctc cgactacaac
      721 gcctaaaagc acgagaagcc cggctttgat ctcggaaaca gtttccccgt ttactactac
      781 tttcgcctct ttaacccgtt gaatatgtgc tagcatttga aaaccccttt attaagtcgg
      841 tcttgctctg gtaacagaga ctatgccgcg aatctgcctt aagcggttca tcacattttc
      901 caggtcggca atgctttcga tgtttaccgt aagctggatg cttgccctac cgtcgtcgag
      961 tgccctggca ttaactgcag cagttgtagt tttgttatcg tttagcgtgg tcatgatgga
     1021 ggttaaaaga ttgggtctat cataaccttc gagatggagt tctaccgggt agtaacctgt
     1081 gtcagtctcg tcccagacca cctcgatcag tctttcaggt tccaaatcgt ttacgttggg
     1141 acagtccttg cggtggaccg taactccccg tcctctggtg atgtagccga cgatctcatc
     1201 tccaggaaca ggagagcagc atctggcaag tttgatcagc atattgtcgg tgccctttac
     1261 acttaccgcg cctccgggct ttactacctt ttgggtggtg ttcaggagat gcttggtccg
     1321 aagatcgcgc tttcgggcat tgtactcctt ctcgccaacc aactctttca gcacatcagc
     1381 ggagataagc ttgccgtagc caatggcagc atacaggtcg tcaacagagg ttaagttgta
     1441 atctctggcc aactcctcca acctatcggt gaggagaata gacccgggct cccactccat
     1501 gcgcctgcat tctctttgca gtaggtcctt acctttgaca atggagtcct ccctttggag
     1561 ttccctgagc ttttgcctga tgcggtttct agcttttgag gtcttgacga agttcaacca
     1621 atcctggctc ggcgaagagt gctttgatgt gagaatctct acgaactcgc cggttttcag
     1681 ctgatagtcc agtggaacaa tcttcccgtt gaccttgcag cccacacagg tattgccgac
     1741 ctgggtgtgg acttcgtagg caaaatccac cggagttgca cctgtaggca gggttaccac
     1801 gtcgccttta ggcgtgaaga caaaaacctc atcctcaaag aggtcgatct tcagagtctc
     1861 cataaactcc tcaacgtctc tggtctcccg ctgccattct aggatctgtc tgagccaagt
     1921 gaacttctcg ttcacctcat ggcctttgga gccctcttta tacttccagt gggctgcgat
     1981 accgtactct gccgtacggt gcatctccca cgttctgatc tgcacttcga aaggctctcc
     2041 attgtcgccg attaccgtgg tgtggagaga ctggtacata ttggatttgg gcatggcaat
     2101 atagtctttg aaccgcccgg gcatcggttt ccaaagagtg tgcaccgtgc ctaaaaccgc
     2161 ataacagtct cggacggtgt ccacaatcac cctgacggca gtaagatcgt aaatctcttc
     2221 gaactccttg ccctgcttga ccattttcat gtagatactg tagaggtgct tcggcctgcc
     2281 gtagatctcc gctttgatgc ccagctccag caatctctcc tggagcttct ctatgacatc
     2341 attaatgaaa acttcgcgtt ctttgcggtt tttcgctact ttttccacga tgtagtagta
     2401 ctcgtcccga tagaggtagc gaaacgcaag gtcttccagt tcccacttga tgcgccacat
     2461 acccaagcgg tgggccaaag gagcatagat gtccagggtt tcttcggcat ttttcttctg
     2521 ccgttcaggc gacaggtgcc ggagcgtgcg catgttgttc agcctgtccg caagcttgat
     2581 caaaactacc cgcagatctt tagccatagc caaaaacatc tttctgatgt tttctgcctg
     2641 ctgttcttcc ttagacagag ttggaagctt ctccagcttg gtgacgccgt ctacgagaag
     2701 ggctacctgc tggccgaact ccgctttaat ggtttcttct cggatggcag tgtcttcaac
     2761 gacgtcgtgg agcaacccag cgatgatggt gtctacatcg agctcgagat cggcaagaat
     2821 atacgccacc tctaaaggat gctcaatata aggcgctcca gattcgcgga actggccttc
     2881 gtgagccttt ttcgcgaact catacgcctt ctccactgtt ttcagactgt cttcagacgc
     2941 atactgcctc atccgctgca gtagatcacc aatttccata gacactaccc tccttttttc
     3001 ggttttagat aaggagcggt cagaacgact gctccctaat ctattaatat ttaactaagg
     3061 aatagatgtc atagccttcg agtttctcac gaccatttag gtatgtgagc tcaatgatga
     3121 aagccaggct cacaacttcg gctccgagct ttttcaccaa ctcgatggtg gccttggttg
     3181 ttccacctgt tgccagaagg tcgtcacaga tcaggactct ctctccaggt tttaacgcat
     3241 cggagtgaat ctccaaagca tcggtgccat actccagttc gtactcaacc cgctcgactt
     3301 tgcaggggag ttttcccggc ttgcgcacta aaacgaaacc tgcgcctaaa gcgtacgcca
     3361 gcggcgctcc aaccaagaaa cctctggatt cgatacccac gatggtatcg attttttcgt
     3421 ctttgtagcg gtctttaatg gtgtcaatca tcgctttaaa agcttcggga tcttgaaaaa
     3481 gggtggtaat atctttgaaa cttacccctt cttttgggaa attttcaatc gtcctgattt
     3541 tactagcaaa atccatcatt gtacccccaa tactgatatg cccgctgttg gaaatgnnnn
     3601 nnnnnntcaa gggcaggaaa aatcatctag agcgctgcaa cttctcgcgc gtatctatcc
     3661 agttcctgct ttctgattat accctcatta tagcttagcg aggcattcaa gtctagtttt
     3721 tgcttggcat cttcaagcac gtagccggtt ctcccggttc cgttaatttt ggttttagtc
     3781 atcagtccca gctccaggaa aatctgtacc gccgtctcca cagactcctc actgaatggc
     3841 ttgtcgtagc ggttttgcag atcgatggcc gcttgtctga aactttccct ccacaccgat
     3901 ctgtaagcgg tacttttaag atagatgtag atccgccgca gatcgtcgtc tgatgggtac
     3961 cgcaggctgt gagctcgctg tactttggtg agctcttcag ctgtaaagcc gatgcccacc
     4021 cgcaagcagc cctgtgccac ctcccaaaag agtcgccaaa gccagtagtc tcccggcaga
     4081 tacgaaaggg tgcactcttt tgccccggag gcaataaatg ccataagttt gtgatcgtcg
     4141 tcgatctcaa aacccacttt ctctggcacg agttggggtt tagtctctgg catattcaag
     4201 tctcttgtag tcctgaccag ggtaatcctc ggatcgagga gtagcttttt gtgcaggacc
     4261 tcaaaagccg ggattatgcc gcaagaaggg agatcta
//
LOCUS       DAOSDA010000101         4286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_12967_length_4286_cov_2.812101, whole genome shotgun sequence.
ACCESSION   DAOSDA010000101 DAOSDA010000000
VERSION     DAOSDA010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12967_length_4286_cov_2.812101"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLW82_03335"
     CDS             complement(<1..498)
                     /locus_tag="PLW82_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46338.1"
                     /translation="MKRGFRVFAFLLAVLLLALLASAADFEPVTLEVWFTEGNEQFEI
                     IRDLVDQYFTPKTGISIKGQVMLYDDLWQKGLLALASGDTPDVASFGSEWPAEFGVRG
                     GLIDLKKEFGEEFDQLIAKVFPNQMRAVEFMGAYFGIPVRFGFAISFYRTDIFEEKGW
                     EYPKTW"
     gene            complement(653..1804)
                     /gene="nagA"
                     /locus_tag="PLW82_03340"
     CDS             complement(653..1804)
                     /gene="nagA"
                     /locus_tag="PLW82_03340"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOJ46339.1"
                     /translation="MSLKLRGAVVLTEHGREYLDISIRDGRISYLGKDDSYPHEEIDL
                     SGLTVLPGFIDIHLHGGGGYDAMTATYEALNTIAITHAKHGTTAMCPASVAASEEELE
                     GLAAAFKEVVEKGTDGAKILGWHVEGPYMNVETLGAHKEEYVRPASVEEMKKLVEISA
                     DSIKLVTIAPEIEGALDVIRYLKSIGVVATMGHSMATADDFAKAVDAGASHTTHLFNA
                     MRKFHHREPGLIGAALVDDRVTSEVIADGIHVHPVALKLFYKCKGAERGILVSDSIEA
                     VDLPDGEYKLGSLTVYVESGRARLADGTLAGSTLTMERAVKNMIELAGASLEDASRMA
                     SLNPAKLLGLDYRKGSIAVGKDADLVALDDDFNVRLTVVEGRIVYNNLK"
     gene            complement(1844..2554)
                     /gene="rpe"
                     /locus_tag="PLW82_03345"
     CDS             complement(1844..2554)
                     /gene="rpe"
                     /locus_tag="PLW82_03345"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOJ46340.1"
                     /translation="MVALLTNLLSNINQIDPAKPLIAPSLLSADFTDLRSDLKRVASA
                     DLLHLDIMDGVFVPNISFGPLVVEAVAKVTDLPLDAHLMVQNPEIMIRELTALKVQAI
                     TISFEAGGHLHRHIMQIKNSGALAGIALNPGTSFESAVALLPFVDLVLVMSVNPGFGG
                     QSFIPEVLPKIRMLDEYRKEHDKRFFISVDGGVNEKNAGVIVQAGADILVSGSAIFGH
                     SNPAGAIENIKKNGRSRL"
     gene            complement(2548..3375)
                     /gene="rsgA"
                     /locus_tag="PLW82_03350"
     CDS             complement(2548..3375)
                     /gene="rsgA"
                     /locus_tag="PLW82_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOJ46341.1"
                     /translation="MEGVVVKVLAGVITVNTPNGLINCTVKGRLKLDDYPTAGDVVWV
                     EVIDDLGVVTKIYPRRNLLDRPRIANVDQIFIFMAAASPEPNAYLVDRLLVIAEYWKL
                     KTIIGFNKTDLKKCELPEIYRDIGYDVYEFSLLEDGAIGVLREALKDKTTVLAGPSGV
                     GKTTFVNKLLGEQRVTADVSEKTQRGRHTTRVVELLRIPEGGYIADTPGFSRLDVLID
                     DERTLWRYFKEYQNANCRFADCMHLKEPGCSVRDKIHPLRFQSYVELLKEIEVMKPW"
     gene            complement(3412..>4286)
                     /locus_tag="PLW82_03355"
     CDS             complement(3412..>4286)
                     /locus_tag="PLW82_03355"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOJ46342.1"
                     /translation="RIDERNLVYEVDSEVFSPDVPKGHVITQIPEAGRVVKINRNVQV
                     ILSKGPELVIVPDLIGMDIRNAQVMLQAMNLTDGEREYVETDEYPPNTIIDQSPKPNE
                     DIEKGSAVNLTVATASSTRVFKMPSLINITLQEAKLLLGRHGLVLGDTYPDSTSILPE
                     GYIVAQNPLPDSDVTEGTIVDVTISAKSGSVFLQESTEGSGDGDEASQWEISHGVKEY
                     NVLVHVVRSGILRIVVIDETGSKEWFHSSVKEGEVIQRNVSGLGAQGKPDVYVQIWLD
                     GEIWREQVLLDVTR"
BASE COUNT          973 a         1213 c          984 g         1116 t
ORIGIN      
        1 ccacgttttc gggtactccc aacctttttc ttcgaagata tcggtacggt agaagctgat
       61 ggcaaagcca aagcgcaccg gaatgccgaa gtaagctccc ataaactcta cggcccgcat
      121 ctggttgggg aagaccttag ctatcagctg gtcaaactcc tcaccaaact ccttttttaa
      181 atcgattaag cctcctctga ctccgaactc ggctggccac tcgctgccaa agctagcaac
      241 gtcgggagtg tctcccgatg ccaacgccaa aagccccttc tgccagaggt catcgtagag
      301 cattacctgc cccttgatac taatgcccgt cttaggagtg aagtactgat ctacaagatc
      361 gcgaatgatt tcgaactgtt cattgccctc ggtaaaccac acttccaaag ttactggttc
      421 gaagtcggct gcagatgcga gcaaagcaag tagcagaaca gctaagagaa aagcgaatac
      481 cctgaaaccc cttttcatgt taaacctcct tctcgttgac tttttgattt ctgctcccaa
      541 ctctcgcaac tctcaaaatc tactctctat tctaagcgtt ctgctcgtga cttttttttc
      601 cttcaataga atacgaaaaa gggaaccgac ttatccatcg gttccccaaa atttacttca
      661 agttgttgta gacgattcgg ccttccacta cagtcagtct aacgttgaaa tcgtcgtcta
      721 aggcaacgag atccgcatct ttgcctacgg cgatgctgcc tttgcggtag tcaagaccca
      781 acagctttgc cggattgagg cttgccatgc ggcttgcatc ttccagtgaa gcccctgcaa
      841 gttcgatcat gttcttaact gccctctcca tggttagtgt cgatcctgca agagtgccgt
      901 ctgcaaggcg cgctctgccg ctttctacgt agactgtaag actccctaac ttgtactcgc
      961 catcgggcag gtcaactgct tcgatagagt cgctgaccaa aatacccctc tcagcgcctt
     1021 tgcacttgta gaacagcttc aaagccaccg gatgaacgtg aatgccgtcg gcgataacct
     1081 ccgaagttac ccggtcgtcc acaagagcag cacctatcaa gcccggctcc ctgtgatgga
     1141 acttgcgcat ggcgttgaac aggtgggtgg tgtggctggc tcctgcgtcc accgctttag
     1201 caaaatcgtc ggcggttgcc atgctgtggc ccattgttgc cacaacgccg atgctcttca
     1261 agtatctgat cacatcaaga gcaccttcta tctccggtgc tatggtcacc agcttgatgc
     1321 tgtcagcgga gatctccacc agcttcttca tctcttctac tgaagcgggg cgtacgtact
     1381 cttccttgtg cgctccgagg gtttccacat tcatgtacgg gccttctacg tgccagccga
     1441 ggatttttgc cccgtcggta cccttctcca caacttcctt aaatgctgct gccaaacctt
     1501 ccagttcttc ttcagaagct gcaacgcttg caggacacat tgccgtagta ccgtgcttgg
     1561 catgggtgat tgcaatggtg tttagagcct cgtaggtggc ggtcatggcg tcatagcctc
     1621 ccccgccgtg aagatgaata tcaataaagc cgggaaggac tgtcagcccg ctgaggtcga
     1681 tctcttcgtg gggatatgaa tcgtctttcc ctaagtagct gattctgccg tctcttatcg
     1741 aaatatccaa atattccctg ccgtgttctg tcagtactac cgctcctcgc agtttcaaac
     1801 tcatcaacat gccctccatt tccgtatagc acgcaagctg cggctataac ctagatcttc
     1861 cgtttttctt gatgttttct atggcacctg ccgggttgct gtgcccgaaa atcgctgacc
     1921 cactgaccag aatgtcggcg ccggcctgca caatcactcc ggcgtttttc tcgtttaccc
     1981 cgccgtctac agagataaaa aagcgcttgt cgtgctcctt gcggtattcg tcaagcatcc
     2041 taatctttgg caggacctca ggtataaagc tctgcccgcc aaagcccgga ttgacgctca
     2101 tcaccagaac caaatccaca aacggtaaaa gcgcaactgc gctttcaaaa gacgttcccg
     2161 gatttagagc aatccctgca agagctccgg agtttttaat ctgcatgatg tgacggtgca
     2221 gatgaccccc ggcctcgaaa gagatggtga tggcctgcac cttgagcgct gttagctccc
     2281 taatcatgat ctcgggattt tgcaccatta ggtgggcgtc gagaggtagg tccgtaacct
     2341 ttgctacggc ctccaccact agggggccga acgagatgtt cggcacaaaa accccatcca
     2401 taatgtccag gtgcaggaga tccgccgacg ccactcgctt gaggtcagat cgcaaatcgg
     2461 taaaatctgc cgacaggagt gaaggtgcaa tcagcggttt tgccggatct atttgattga
     2521 tgttggacaa caggttggtc agaagtgcta ccatggcttc atcacctcta tctccttcaa
     2581 aagctctacg tagctttgaa agcgcagtgg atggatttta tcccggacac tgcagccggg
     2641 ttccttcaga tgcatacagt cggcaaagcg acagttggcg ttttgatact ccttaaagta
     2701 acgccagaga gttctctcat catcgattag cacatccagc ctggagaaac caggtgtatc
     2761 tgctatatac ccaccctccg ggattcttaa aagctcaacc actctggtgg tgtgccttcc
     2821 cctctgggtt ttttcactca catcagcagt tacccgctgt tcacctagga gtttgttgac
     2881 aaaagtggtt ttgccgaccc ccgaaggacc agctaaaacc gtcgtcttat ctttgagagc
     2941 ttccctgagg acgccgatgg cgccgtcttc caggagactg aactcgtaga catcgtagcc
     3001 gatgtcgcgg taaatctcag gtagttcgca ttttttcaag tcggttttgt tgaacccgat
     3061 tatggtcttg agcttccaat actctgcgat caccaggagg cgatccacaa ggtaggcatt
     3121 aggttctgga gatgccgctg ccatgaagat aaagatctga tcgacgttgg caattcgcgg
     3181 tctgtccagg agatttcttc tggggtagat cttggtgacc acaccgagat cgtctatcac
     3241 ttctacccaa accacatctc ctgcagtagg gtaatcatcc agctttaagc ggcctttcac
     3301 cgtacagtta atcaggccgt tgggtgtatt aactgtaatg acacctgcta gaacttttac
     3361 cactactcct tccataaact atcctccttg ctgcaaaaaa cgacagaata cctatcttgt
     3421 aacatctaag agcacctgct ccctccaaat ctctccgtca agccagatct gcacgtacac
     3481 atcgggcttg ccctgagcgc ccaaaccgct cacgtttctc tggatcactt cgccttcctt
     3541 cacgctgctg tggaaccact ctttagaacc ggtttcatcg ataacgacga ttctcaagat
     3601 accacttcta accacgtgaa caagaacgtt gtactccttg actccgtggg agatctccca
     3661 ctggcttgct tcgtccccgt ccccggaacc ttcggtgctc tcctgaagga acaccgaccc
     3721 cgatttagcg ctgatggtaa cgtcgacgat ggtgccctca gtaacgtcgg agtccggcag
     3781 cgggttctga gcaacaatgt agccctcagg caaaatcgaa gtgctgtcgg gataggtatc
     3841 ccctagaaca agtccgtgcc ttcccagaag aagcttggcc tcttgcaggg tgatgttgat
     3901 cagacttggc attttaaaca ctctggttga actggcagtt gccacagtca aattgaccgc
     3961 actgcccttt tctatatcct cgttaggctt tggcgactgg tcgataatgg tgtttggcgg
     4021 atactcgtct gtctctacgt actccctctc accgtctgtt aggttcatcg cctgcagcat
     4081 tacctgggcg ttgcgaatat ccatgccgat gagatcagga acaataacca gctccggacc
     4141 cttagaaagg atcacctgaa cattacggtt tatcttcaca acccgccctg cttcaggtat
     4201 ctgggtgatg acgtggcctt tgggaacgtc cgggctgaag acttcagagt ccacttcgta
     4261 gaccaggttg cgctcgtcga tccttt
//
LOCUS       DAOSDA010000102         4252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13102_length_4252_cov_3.651179, whole genome shotgun sequence.
ACCESSION   DAOSDA010000102 DAOSDA010000000
VERSION     DAOSDA010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13102_length_4252_cov_3.651179"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..299
                     /locus_tag="PLW82_03360"
     CDS             111..299
                     /locus_tag="PLW82_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46343.1"
                     /translation="MNRHLLRRDQIYFVEKDDYGASDLYSLYDVDLDIRSNFNYEDNY
                     LAGRFGAIPYVSDFCSTK"
     gene            317..937
                     /locus_tag="PLW82_03365"
     CDS             317..937
                     /locus_tag="PLW82_03365"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HOJ46344.1"
                     /translation="MAKGRRTSSYRRRKRGIRPNPFFIIVCEGEVTEPDYFKSFPYSC
                     KLGYSYVHGTVHIVSGAGQHEKVVVKADEVWRKLKKEYGAISSNEVWCVFDCDGNTDS
                     LNRAIKAAESKGFNAIYSIQCFELWFLLHFQPLTTAIDRKDYDRRLSNHLGINYEHGM
                     KGMYHLLQNRQEDAIRAAKQLWEQKAELGELTGDPITNVFMLVEAL"
     gene            complement(1251..1733)
                     /locus_tag="PLW82_03370"
     CDS             complement(1251..1733)
                     /locus_tag="PLW82_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOJ46345.1"
                     /translation="MSKEWVKNVSQQTLEQFKRAFILKCMETGSDRLQVKYDELAEIC
                     GLSKGAVFKAVEVLTEQGFIERIPAESRRIANTYVLKGPYSVDIPKSTDKFAFGSGAD
                     LVTKVASIRAKMKELTDEVQDLRALSSLTYGGDIEVVAKNTLPGDYIQLIYKVNKQDQ
                     "
     gene            complement(1960..3987)
                     /locus_tag="PLW82_03375"
     CDS             complement(1960..3987)
                     /locus_tag="PLW82_03375"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOJ46346.1"
                     /translation="MNRLKREPSPYLQLHGENPIDWYPWGEEAFARAKALDRPIFLSI
                     GYSTCHWCHVMNDESFQDEEVAEILNEHFVAVKVDREQHPDVDAVYMDYCVRTTGMGG
                     WPLTVFLTPDLLPFYAGTYFPKKATLRRIGLIDVLQKIVEFWENDRSIIDEAAQRSIE
                     NLPEAGGALGEFVVEAALKAVENLKEEYDPEHGGFLPAPKFPQLQNLLLLLDAAADSS
                     DHKTLHIAMHTLKSLLGKGTFDQVGGGMFRYSTDAQFKVPHFEKMLYDNAFLLYTLGE
                     FYRFTKDRYFKHKAIQVFEYLEANLKGEDGGYFTAQDAGTKEGAFYLYTVGELEEVLT
                     PAELRLLAKYYEISADGNFEGKIHLFPKSSKAFWDLVSGKDREELEQIQSKLKRYRGK
                     TRTAPFTDKKILVFANGLLLAGLAKAAFLDERIQNAADALYRYLRGIAASEELPGALI
                     GGKVISKGHLDDYAYLAWGFFEYGVMKEGRERKEALEIALNLVNRANRLFYDEEAGGY
                     YKIAADTILPTRPKEVFDGAIPQGNSVMSLVLFRLFQVTGDLELRRRLEQTLRGLAGV
                     LKSDPEGAPLLVLVTSWYLGESWEVVLTGGEKDAGFLAQIRSEIQQNYLAGILFWFAD
                     GTCPEFGIEAGKPAGELALYLCKGGTCSERINSEAEIRAALAKLFPGKFQQ"
BASE COUNT         1102 a         1056 c          931 g         1163 t
ORIGIN      
        1 gcttctttta tttcatgggg aaaccaattt tacacaatta tacggactca acttcaaaaa
       61 ctaatccacg taacgcgcaa ttgctcttca ccactcacaa cactttcctg atgaatcgtc
      121 atctattgag aagagaccag atctatttcg ttgagaaaga tgactatgga gcaagcgatt
      181 tgtacagctt atacgatgtt gacctagaca ttcgcagtaa cttcaactat gaagacaact
      241 atcttgcagg gcgcttcggt gctataccgt atgtaagcga tttctgttca actaaataaa
      301 tggatggggg attgctttgg ctaaagggcg cagaacatcc agttatcgga gaagaaaaag
      361 aggcatccgc cccaatccat ttttcatcat agtctgtgaa ggtgaagtta cggaacctga
      421 ttactttaag tcatttccat attcatgcaa acttggttat agctatgttc atggaacggt
      481 ccacattgtc tccggagcgg ggcaacatga aaaagtagtt gtaaaggccg acgaggtttg
      541 gcggaaactc aaaaaggagt atggggcgat tagttcgaac gaagtatggt gcgttttcga
      601 ctgtgacgga aatacggata gccttaaccg agcaattaaa gctgcagagt ctaaaggctt
      661 caacgcaatt tactcgatac aatgctttga gttatggttt ttattgcatt ttcaaccact
      721 cacaacggct atagatagaa aagactatga cagacgactg agtaaccact tggggatcaa
      781 ctacgaacac ggaatgaagg ggatgtacca tctcttgcag aaccgtcagg aagatgccat
      841 tagagctgcc aagcagctgt gggagcagaa agcagagctt ggtgaactca ctggtgatcc
      901 tattacaaat gtgttcatgc ttgttgaggc attatgatgc tcatcgcaac cttaaaaaca
      961 gaagatgatt cgaatcggtt cttaatcggt tttgggttga caagcgagag actttgttaa
     1021 tttgctatgt tttcagctcc aaaacacgca gcctaagcag aattctggca taccgctgct
     1081 gtctagatcc taccaaagtc ttagtagaat gaaaaacgct gtcttagttt attaacaagc
     1141 ttttttgcga cgagccttct ttggctcgtt tctttttgtg cttacaaaca aaggctgctg
     1201 cacaacttgc ttgggtggct aaatgtgcaa cagccctttt gtagtatatt tcactgatct
     1261 tgtttattta ctttgtagat cagctggatg taatcaccag gaagagtgtt cttagcaaca
     1321 acctcaatat ctcccccgta cgtcaaagat gaaagagctc tcaggtcctg gacttcatct
     1381 gtcagctctt tcatctttgc cctgatacta gcgactttag ttacaagatc agcaccggag
     1441 ccaaaagcaa acttatcggt gctttttggt atatctaccg aatagggtcc tttcaaaacg
     1501 taagtgttgg ctattctgcg gctctcggct ggaattcttt cgatgaaacc ctgttcagtc
     1561 aacacttcaa ctgctttaaa cacggctcct ttagaaagac cgcaaatctc ggccagctcg
     1621 tcgtacttga cctgcagccg atcacttcct gtttccatgc acttaagaat gaatgctctt
     1681 ttgaactgct ctagcgtttg ctgcgatacg ttcttgaccc attccttaga cacttcagtt
     1741 ccccattcac ctcctggaaa taatcggtat caagatacca gcctcaagtc ctattatagc
     1801 acatttgtgc ctaaaaaaca gccaatatac tcaaattgat tactatttat tattacatta
     1861 tacaattatc cagaaggtta tttggttcaa ttcaggcatt aaaaacttgt tgatccattg
     1921 cgaaaagcag ctcacaggcc attgtcccca agcttgtttt cactgctgaa acttcccagg
     1981 aaataatttt gcaagtgcgg ctctgatctc ggcctctgag ttaatcctct cgctgcaggt
     2041 tccccccttg caaagataga gggcaagctc gcctgcaggt ttccccgctt caatgccaaa
     2101 ctcaggacag gtgccatctg caaaccagaa cagaatgcct gcaagatagt tctgttggat
     2161 ttcggatctg atctgggcca aaaaccctgc gtccttctct cctcctgtta gcaccacttc
     2221 ccagctttcg cccaggtacc agctcgtaac aagaaccaga agcggtgcac cttcagggtc
     2281 gcttttgagc actcctgcaa ggccgcgcag ggtctgctcc aaccttctcc gcagttcgag
     2341 gtcgccggtc acctgaaaga gcctaaacag cactaaggac atcaccgagt tgccctgggg
     2401 aatagctccg tcgaacacct cttttggccg tgtgggaagg attgtatctg cggcgatctt
     2461 gtagtaacct cctgcttctt cgtcgtaaaa gagccggttg gctctattca ccaggttcag
     2521 cgcaatctcc aacgcctctt tcctttccct accctctttc atgacaccgt actcaaaaaa
     2581 gccccaagca agataagcat agtcgtcgag gtgacctttg gatataacct tgccccctat
     2641 caacgctccc ggaagttctt ccgaagcggc aatccctcga aggtagcgat acagagcatc
     2701 tgcagcgttc tggatccttt catcaagaaa agcagccttg gccaatcctg caaggagtaa
     2761 accgttggca aacacgagaa tctttttgtc ggtgaaagga gcggttctgg ttttcccgcg
     2821 gtaccttttc agcttacttt ggatctgttc gagctcttcc cggtccttcc ctgatacaag
     2881 gtcccaaaaa gcttttgagc tttttgggaa aagatggatt tttccctcga agtttccatc
     2941 tgcagagatt tcgtagtact tggccagaag acgaagctcg gctggagtga gaacctcttc
     3001 tagttctccg acggtgtaaa gatagaaagc tccttcctta gtccctgcgt cttgagctgt
     3061 aaaatatcct ccatcttctc ctttgagatt ggcctctaag tactcaaaaa cttgaatggc
     3121 cttgtgttta aagtatcggt ccttggtaaa gcggtagaac tctcccagag tatagagtaa
     3181 aaaggcgttg tcgtagagca tcttttcgaa gtgaggaact ttgaactggg catcagtaga
     3241 gtatctgaac ataccccctc ccacttgatc gaaagtgccc ttgccaagaa ggctctttag
     3301 ggtgtgcatg gcgatgtgca gcgttttgtg atcgctgctg tctgcggcag catccagaag
     3361 caaaagaaga ttctgcagtt gaggaaactt tggtgcaggc aaaaaccctc cgtgctcagg
     3421 atcgtactct tccttcaggt tttccacagc cttcaacgca gcctctacca cgaactctcc
     3481 caaagcacct ccagcctccg gtaagttttc aatgctccgc tgtgcagctt catcaataat
     3541 actccggtcg ttctcccaga actcgacgat tttttggagc acatcgatca ggccaatccg
     3601 gcgcaatgta gcctttttcg ggaagtaagt ccccgcataa aacggcagca ggtccggagt
     3661 gagaaacacc gtcagcggcc atcctcccat accagtagtt cttacgcagt agtccatgta
     3721 cacagcatct acgtctgggt gctgctctcg atctacttta acagctacga aatgctcgtt
     3781 gaggatctct gccacttcct catcttggaa ggactcgtcg ttcataacgt ggcaccagtg
     3841 gcacgtggag tagccgatgg aaagaaatat tggacggtcc agcgctttag cccgtgcaaa
     3901 ggcctcctcg ccccacggat accagtctat ggggttttct ccgtggagct gcaggtacgg
     3961 tgaaggttcg cgttttagcc gattcatagc tatcccccat ctgttttgac atctcttatg
     4021 gtgtaattat aagataccca actgcacagt ttccatggct ctgttttgga gaaaaggggc
     4081 gaagtacata cacactgcaa gcgatcccca ccgctctctt agaatgcctg caatctcaaa
     4141 ctccgagtct ccgcatgtcc ggataacata gactaaaaaa gagcacgttt gcaacactaa
     4201 gcaccactgg tgatttgtaa gggtcattac tactccatat ctcgaggctg ga
//
LOCUS       DAOSDA010000103         4251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13106_length_4251_cov_3.548618, whole genome shotgun sequence.
ACCESSION   DAOSDA010000103 DAOSDA010000000
VERSION     DAOSDA010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13106_length_4251_cov_3.548618"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PLW82_03380"
     CDS             complement(<1..951)
                     /locus_tag="PLW82_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOJ46347.1"
                     /translation="MSFLMALLILIALFSLSENGDHPKRRRSRRHILYTESDSCLEEC
                     CKRAHSIGIKIVKELPFIGGVVCETADGEDVKLLTLERDVKIEEDVKVYATCLGFGRD
                     QAAVGAIPPDTITLTGAPNLWKKTTGKGVSIAVIDTGIAEHSDLRVKGGISTLGEVHT
                     ENFRDDNGHGTHVAGIIAACGRDQGLVGMAPDADLYAVKALDRYGSGFVSDIIEGIAW
                     ATEHRMNIVNLSLGTAEKSEALRRAVRKAVAASTILVAASGNSGGTGSPGVLYPAKFS
                     EVVSVGSVDSDGKLSSYTSYGPELNILAYGENTLSTYLNNS"
     gene            complement(951..1169)
                     /locus_tag="PLW82_03385"
     CDS             complement(951..1169)
                     /locus_tag="PLW82_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46348.1"
                     /translation="MSNLINEDIIKQIRARLEKMPAEQRKKLAEDAMRLVGTNDPEKL
                     KEILKNQLPNMDPNLLQETLDKLLKENM"
     gene            complement(1282..1593)
                     /gene="trxA"
                     /locus_tag="PLW82_03390"
     CDS             complement(1282..1593)
                     /gene="trxA"
                     /locus_tag="PLW82_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOJ46349.1"
                     /translation="MPIDLTDLNFEKEIASGVTLVDFWAPWCGPCRMMAPIIENLAKE
                     FEGRAKIAKMNVDENPMTPSKFHIQGIPTMILFKDGKAVDMVVGYTRQQELSAKLNNL
                     L"
     gene            1846..2295
                     /locus_tag="PLW82_03395"
     CDS             1846..2295
                     /locus_tag="PLW82_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46350.1"
                     /translation="MNKPFIVVLGKPGGGKSSVLRQSFSEVRDSAAFLDGDHDFEQMM
                     HLVHECGFQRSSYFYCPVPEELPSIVEERTEPFIFIDTPRLTEDIVTKVAKAAKNKKV
                     VLAVTSTDESLPSELEPLVSDVIILRENKEEVKKAIVELLESSMENV"
     gene            complement(2474..3856)
                     /locus_tag="PLW82_03400"
     CDS             complement(2474..3856)
                     /locus_tag="PLW82_03400"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003336280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOJ46351.1"
                     /translation="MTLTMERLTNLCKTRGFIFPGSEIYGGLANSWDYGPLGVELKNN
                     IKKAWWKKFIQESPYNVGVDAAILMNPKTWVASGHVGGFSDPLIDCRKCKERFRADKL
                     IEDYNRSQNIDIQVDGWSNQKMESYIEEKAIPCPSCKSHDFTPIRQFNLMFKTFQGVT
                     EESKNEIYLRPETAQGIFVNFKNVQRATRKKLPFGIGQIGKSFRNEITPGNFIFRTRE
                     FEQAELEFFCKPGEDLKWFEYWKSFCYNWLLGLGIPEEDLRIRDHSQEELSHYSVATS
                     DIEYRFPFGWGELWGIADRTDFDLTQHMEHSGEDFHYFDQETNEKYVPYVVEPSVGVD
                     RLLLMVLSSAYKEEELSDGTTRSVLKFHPVLAPFKAAVLPLTKKLSEPATEIYHSLMK
                     DFMVDYDESGSIGKRYRRHDEIGTPYCITVDFDTLENQTVTVRDRDSMEQERIHINEL
                     KAYLAEKTAY"
     gene            complement(3870..>4251)
                     /locus_tag="PLW82_03405"
     CDS             complement(3870..>4251)
                     /locus_tag="PLW82_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ46352.1"
                     /translation="LLFERMPNVSWVGFYLLKSSVLMLGPFQGRVACVRIDIGKGVCG
                     TAFIKQETIIVPDVHEFPGHIACDPRSNSEIVVPLFKDGRVIGVLDIDSTSKARFTTD
                     DQVMLEKVAQIVTANCNIEEFVDN"
BASE COUNT         1053 a          981 c         1009 g         1208 t
ORIGIN      
        1 gctgttgttc aggtacgtgg aaagggtgtt ttcaccgtaa gccaggatgt tcagttctgg
       61 cccgtatgat gtgtagctag agagcttgcc atcggagtca acggacccta cactgaccac
      121 ctcagagaat ttggcaggat acagaactcc ggggctgccg gtaccgccgc tgttgcctga
      181 tgcagcaaca agaatagttg atgcagcgac cgctttcctg acggctctcc gcagagcttc
      241 gcttttttcg gcagtaccaa ggctgaggtt aacgatattc attctgtgtt ccgtagccca
      301 agcaatgcct tcgataatat ccgaaacaaa accactgcca tatcgatcta aagctttaac
      361 agcgtagaga tcagcatcgg gagccatgcc tacgaggccc tgatctctgc cgcaggctgc
      421 aatgatacca gcaacatgag tcccgtgacc gttgtcatcg cgaaagtttt cagtgtggac
      481 ttcgcctaaa gttgaaatac cgcctttgac cctaagatca gagtgttctg ctatgcccgt
      541 atctataacc gcaatggaaa cgcccttacc tgtggtcttt ttccaaagat ttggagcgcc
      601 tgtgagggtt atggtgtcgg gaggtatcgc tccgacagcg gcttggtccc tcccaaaacc
      661 caggcaagta gcataaactt tgacgtcctc ttcgattttc acgtctcttt ccagagtgag
      721 aagctttacg tcctctccgt ctgccgtttc acataccacg ccgcctataa atggcagctc
      781 tttgactatt tttatgccaa tagaatgggc ccttttgcag cattcttcga gacaggaatc
      841 agattcggta tataggatat gacggcgaga tcttctcctt tttggatgat ccccgttttc
      901 agacaaggag aaaagtgcaa tgagaatgag cagagccatg agaaaactca ttacatattc
      961 tcctttaaca gtttatccag agtttcttgc agcaggtttg ggtccatgtt tggcagttgg
     1021 tttttcaata tctctttgag cttttcggga tcgtttgtac caacaagccg cattgcatct
     1081 tcggctaact tttttctctg ctctgcaggc atcttctcca atcgagcgcg gatctgcttg
     1141 ataatgtcct cgttgatcaa attgctcata aaaacgcctc ctataagctg cgttaatcta
     1201 taataggcgt gagaaacaaa aaaagtgcct atctgcagct cacaagaggt cacaaaaaaa
     1261 aggcctatat caggcctttt tctatagcag gttgtttagc tttgcgctca actcttgctg
     1321 tctcgtatat cctactacca tgtctacggc tttcccgtcc ttgaacagga tcatcgttgg
     1381 gattccttga atgtggaatt tagacggtgt catggggttt tcatcgacgt tcatcttggc
     1441 gatttttgcc cgtccttcga actcttttgc aaggttctcg atgatcggtg ccatcatgcg
     1501 gcatggtccg caccaagggg cccaaaaatc taccagggta acaccacttg cgatttcttt
     1561 ttcaaaattg agatctgtca aatctatagg catggaaaaa cctcctcaac cactttttat
     1621 gatttaagta tatgcctata tgcttaaatt gtcaagccca aatcctgtct tatttttcgt
     1681 caataagtct acaaaaatta tcgtttaaaa tctcgttgtg tgtattagaa accaaactgg
     1741 gtatatttaa aagtacaagg tacggtttta aattcgcgtg tcgctaaagg aaaaaagttt
     1801 cttgtaaaga aatctttaac taagaagaaa gaaagggtgg tggctgtgaa caaaccattt
     1861 attgtcgtcc tcgggaaacc tggaggcggg aagtcgagtg tgctccgtca gagcttttca
     1921 gaggttcgcg attcggccgc tttcttggat ggcgaccatg attttgaaca gatgatgcac
     1981 ctggttcatg agtgcggatt tcagagatct agttattttt actgcccagt tccggaagag
     2041 ttgccatcaa tagtggaaga aagaaccgaa ccgttcatct tcatcgacac gcccaggttg
     2101 accgaggaca ttgtgacgaa ggttgctaaa gcagcgaaga acaagaaagt ggtcttggct
     2161 gtcacatcaa cggacgaatc actaccttca gaactggagc ccctagtttc tgatgttatt
     2221 atactgcgtg aaaataaaga agaagttaag aaagccattg tggagctgct tgagtcctcg
     2281 atggagaacg tgtgatgcct acctttacgg aaggcttttt ctgtctataa gagcgggagc
     2341 ttgatcgaac cggcaggaaa gcccaagctg cagtgacaac accagaaatt ggtaggaata
     2401 ccttcgatga tgaaaaaagg cacttgatct ttgcagcatc aagtgccttt cctgtctctg
     2461 tattaaacac tgcttagtag gcagtttttt ctgctagata ggcttttagt tcgttaatgt
     2521 ggatgcgctc ttgctccata gagtctctgt ctcttacagt gactgtctgg ttttccaggg
     2581 tatcgaaatc gacggtgatg cagtaaggtg tgccgatctc gtcgtgcctg cggtatctct
     2641 tgccgatgga gccagactca tcgtagtcga ccatgaagtc cttcatcaag ctgtggtaga
     2701 tttctgtggc aggctcagag agcttcttcg taagaggaag gactgctgcc ttaaacggag
     2761 caagtaccgg gtggaactta aggaccgatc ttgtggttcc gtcgcttagt tcctcttctt
     2821 tgtaagcgga agaaaggacc ataagcagca atctatctac gccaacagac ggctcaacga
     2881 cgtaaggtac gtacttttcg ttggtttctt gatcgaagta gtggaagtct tcgccggagt
     2941 gctccatgtg ctgagtgagg tcgaagtcgg tgcgatcggc aatgccccac agttcgcccc
     3001 aaccaaatgg gaacctgtac tcgatgtccg aagtagccac agagtagtgg gacagctctt
     3061 cttgagagtg gtctctgatt cttaagtctt cttctggtat tcctagacca aggagccagt
     3121 tgtagcagaa gctcttccag tactcgaacc acttgaggtc ttcaccaggc ttgcagaaga
     3181 attccaactc tgcctgttcg aactcgcgag ttctgaagat gaagtttcca ggtgtgatct
     3241 cgttgcggaa gctcttaccg atttggccaa tcccgaaagg aagtttcttc ctagtagctc
     3301 tctggacgtt cttaaagttc acgaagattc cttgagccgt ttcaggacgg aggtagattt
     3361 cgtttttcga ctcttccgtt acaccctgga aggttttgaa catgaggttg aactggcgaa
     3421 tgggcgtgaa atcatggctc ttgcagctgg gacaggggat ggctttttct tcgatgtagc
     3481 tttccatctt ctgattagac caaccgtcta cctgtatatc gatgttttga gaacggttgt
     3541 agtcttcgat gagcttatct gcacggaacc tttctttaca tttgcggcag tcgatcagcg
     3601 gatctgagaa gccgccaacg tgacctgacg caacccacgt tttaggattc atgagaatag
     3661 cggcgtccac acctacgttg tagggggatt cttggatgaa cttcttccac caagcttttt
     3721 tgatgttgtt tttaagctcg actcccagcg gaccatagtc ccaggagtta gccagtccgc
     3781 cgtagatttc cgaacctgga aagataaaac cacgggtctt acataggttt gttagacgct
     3841 ccatagtcag tgtcatgtca aaactcctct cagttatcaa caaattcttc aatattgcag
     3901 ttagccgtta ctatttgggc aactttttcc agcatcactt ggtcatctgt tgtaaatctt
     3961 gctttagaag tgctgtcgat gtccaagacc ccgatgaccc tgccgtcttt aaacagggga
     4021 acaacgatct cagagtttga ccgaggatcg caggcgatat gccctggaaa ttcgtgaaca
     4081 tcgggcacaa ttatagtctc ttgttttata aaagccgtgc cgcagacacc tttgccgatg
     4141 tctatgcgga cgcaggctac tcttccttgg aatggaccaa gcattaagac ggagcttttg
     4201 agaaggtaga aacccaccca gcttacattg ggcatccttt caaaaagcag t
//
LOCUS       DAOSDA010000104         4250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13111_length_4250_cov_3.565435, whole genome shotgun sequence.
ACCESSION   DAOSDA010000104 DAOSDA010000000
VERSION     DAOSDA010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13111_length_4250_cov_3.565435"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /gene="cheB"
                     /locus_tag="PLW82_03410"
     CDS             <1..912
                     /gene="cheB"
                     /locus_tag="PLW82_03410"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOJ46353.1"
                     /translation="QMPRMDGLTFLKKQMEIKPIRVVMVSSMTSEGAAITIDALSSGA
                     IDFVEKPSGPLSLDMQKVADEMCRKVKAAHKARIHLPSRLTRETDERPRYQKPETYEA
                     PKKEDEGFKKLPTKTANKLVIIGSSTGGPSALMEVIPRLPKDLGAGVVVVQHMPRGFT
                     KSLAERLNRASALYVKEAETGDVILENQVLIAPGGFHLVLSGNKEVKLNTEPPVHGVR
                     PAIDVFIESAAKVFKEHTVGVILTGMGFDGAKGMKLIKELGGKTIAQDEKTSVIYGMP
                     RAVVDMGLADRVAPLDSVSTFIEELLR"
     gene            909..1691
                     /locus_tag="PLW82_03415"
     CDS             909..1691
                     /locus_tag="PLW82_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOJ46354.1"
                     /translation="MRDEYSVFCSEIYRITGINLALYKDQQMKRRLNTFMHKLRVESY
                     DQLAKKIEKDQTALKMFHEFVTINVTDFFRDNEQFETLRKRILPQLVKSGSPFRAWSA
                     GCSDGREAYSLLMMLEEMGVTDYQIHGTDLDERILEKARQGIYNEEAVHKVPAHLLAK
                     YFTKLPDGNYQFAPEKARKVQFYKQDLLADEYRKGFDLIVCRNVVIYFTEEAKDFVFA
                     KMAKSLKPDGILFVGGTESILAPKALGLTPVLPFFYKRDDSM"
     gene            1744..2211
                     /locus_tag="PLW82_03420"
     CDS             1744..2211
                     /locus_tag="PLW82_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOJ46355.1"
                     /translation="MKTEFVTPFMVAATDVLKTEVQAEITRGELTVRKVATTAQEVTI
                     MVGVTGDVAGAVFYGMANKTACQIVSVMLGQRLPVFDEIVESGIAELGNVITGRASMG
                     LEEAGYSCRLTPPTIISGQGVIISTLEINQLVIPIKTQYGDIEIGVALREAQS"
     gene            2227..2979
                     /locus_tag="PLW82_03425"
     CDS             2227..2979
                     /locus_tag="PLW82_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOJ46356.1"
                     /translation="MLTIADILDDLRKLGIKEGDRVLVHTSLSKVGMLEDGPAAFVEA
                     IVSAVGNEGLAVFPTFTGTAQDSAENPPYFDVRETPCWTGKVPEAARRREDAIRSLHP
                     THSVVAIGADNKQLVKDHEKVETPCSHGSPFMKLAENSGKILLVGVDHNSNTTFHTAE
                     ELADCPYHMLPEPALATVVDYQGKEHKILTALHKWGTERDFNRLEEEFIKQGIQRNGK
                     IGNALCRLIDTKPMLEVAVAILKKDPYFLVVN"
     gene            2999..3697
                     /locus_tag="PLW82_03430"
     CDS             2999..3697
                     /locus_tag="PLW82_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ46357.1"
                     /translation="MKQTGTRQSRKHYLKLLLVAAAIVVLVSSSAFAAGILRQGKASN
                     DGAKRIALTFDDGPYPEITPAILKALEEADVKATFFVVGWRVYEYHDIFTTMNEKGHE
                     VGNHSYDHPHLTSLTAEEIASQLKKTNYAVQKYSGKKPVYLRPPYGSYNDLVLEIAAE
                     NDMKVVLWSLNPEDWASPGVDVIISRVLEQASDGSVVLLHDTWQTAQMLPELITTLKE
                     RGYVFVTLSELFAD"
     gene            3979..>4250
                     /locus_tag="PLW82_03435"
     CDS             3979..>4250
                     /locus_tag="PLW82_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46358.1"
                     /translation="MYGEQESVDALQTALLSQLEVTDEGDLHYWLGEALLEYSDDPVV
                     IREGIYHLVHSLRLLSQTSEDNTYGKAKLKRNCLSTAMRLRQIRNGR"
BASE COUNT         1255 a          936 c         1082 g          977 t
ORIGIN      
        1 cagatgccaa ggatggacgg gctgactttt ttaaagaagc agatggagat caaacccata
       61 cgagtggtca tggtaagcag tatgaccagt gaaggggcag caatcactat tgacgctctg
      121 tccagtggcg ccattgactt cgtagagaag ccttctggac ccttatcgtt ggacatgcag
      181 aaggtggccg acgaaatgtg tcggaaggtt aaagcagctc acaaagctag aattcacctg
      241 cctagcagac tgacaaggga aactgacgag cgtcctcgtt atcaaaagcc tgaaacatac
      301 gaagctccta aaaaagagga cgaaggtttt aaaaaactac cgaccaaaac cgccaacaaa
      361 ctggttatca tcggatcttc gacaggaggt ccaagcgcat tgatggaagt aattccgagg
      421 ttgccaaaag accttggagc aggcgtggta gtagttcagc atatgcctag aggatttacc
      481 aaatctctgg ctgaaaggct gaacagggct tctgcactat atgtcaaaga agcagaaaca
      541 ggcgacgtaa tactggaaaa ccaagtgctt attgcaccag gcggcttcca tttagtgctt
      601 tcaggaaaca aagaagttaa actcaacaca gaacctccgg tacacggagt gcgacctgca
      661 atagacgtct tcattgaaag cgcagcaaaa gtattcaaag aacatactgt tggcgttatt
      721 ctcacaggta tgggttttga cggcgcaaag ggtatgaaac tgatcaagga gctgggaggc
      781 aagaccattg ctcaagatga aaagaccagc gtgatctacg gaatgcccag agcagtcgtg
      841 gatatgggat tagccgacag agtagctcca ctcgattcag tcagcacttt tattgaggag
      901 ttgttacggt gagggatgag tacagcgttt tctgctcaga aatatataga ataaccggaa
      961 taaaccttgc actgtacaaa gaccagcaga tgaaacgccg gttaaacacc tttatgcaca
     1021 aattaagggt ggagagctac gatcaactcg caaaaaagat cgaaaaagat caaactgctc
     1081 ttaaaatgtt ccacgagttt gttaccatta atgtaacgga cttttttcgc gacaacgagc
     1141 agtttgagac tctacggaag agaattctgc cgcagctggt gaagtcggga tcaccgttta
     1201 gggcttggag tgctggctgc tctgatggaa gagaagctta ctctttgcta atgatgctcg
     1261 aggaaatggg tgttaccgac taccagattc acggtactga tctggacgag cgtattttgg
     1321 aaaaagctcg gcagggcatc tacaacgaag aagccgtgca caaggttccg gcgcatctcc
     1381 ttgccaagta ctttacgaaa ctaccagacg gcaactacca gtttgctcct gaaaaagcta
     1441 gaaaggttca gttctataag caggatcttt tagcggacga gtacaggaag ggttttgacc
     1501 tgattgtctg ccgcaatgtt gttatctatt ttaccgaaga agcaaaggat tttgttttcg
     1561 ccaagatggc caagtctctt aagcctgatg gcatcttgtt tgttggcgga actgaaagca
     1621 ttctagcgcc caaggctctt ggtctaacac cggtcttacc atttttttac aagagagacg
     1681 attccatgta gcttcttaaa aggctgctac tgttggtaat tactttgaag gagagaagaa
     1741 attatgaaaa cggaattcgt taccccattt atggttgcag caactgacgt cttaaagact
     1801 gaagttcaag ctgagatcac tagaggtgaa ctcacagtgc gtaaggttgc tacgacggct
     1861 caagaagtaa ctatcatggt gggcgtaaca ggcgatgtgg ctggtgcggt tttctacgga
     1921 atggccaaca aaactgcctg ccagattgtc tctgttatgt tggggcagag actgccggtt
     1981 ttcgatgaaa tcgtggaatc tggcatcgcc gagctgggca acgtcatcac cggacgggcg
     2041 tcgatggggc ttgaagaagc tgggtattcg tgcaggctaa cccctccgac aatcatctcc
     2101 ggacaaggcg tgatcatctc tactctagag atcaaccagt tggttattcc catcaaaact
     2161 cagtatggtg atatcgaaat aggagttgca ctgagagaag cacagagcta aaggtggtag
     2221 ggtcagatgc tgacaatagc cgacatacta gacgatcttc gcaaattggg cattaaggaa
     2281 ggcgatagag tgctggtgca cacttcattg agcaaggttg gcatgctcga agatggaccg
     2341 gcggctttcg tagaagcaat cgtttctgct gtaggaaacg aagggctggc ggtgtttcca
     2401 accttcaccg gaactgcgca ggattccgct gaaaatccgc cttattttga tgtgcgggag
     2461 actccgtgct ggacgggaaa agtgcccgaa gcggcaagaa gaagggaaga cgccataaga
     2521 agcctccatc caacccattc agtggtagct atcggcgcag ataacaagca gctggttaaa
     2581 gaccacgaaa aagtagagac tccgtgcagc cacggcagtc ctttcatgaa actggcagaa
     2641 aattcaggta agattttgtt agtcggggtg gatcacaact ccaacaccac atttcataca
     2701 gcagaagagc ttgcagactg tccgtaccac atgcttccag aaccggcttt agctactgta
     2761 gtggattacc aggggaaaga gcacaaaatc ctcacagctt tacataaatg gggaactgaa
     2821 agagatttta acaggttgga agaagagttt atcaagcaag gaattcagag aaacggtaag
     2881 atcggcaacg ccttgtgcag gctcatcgac acaaagccca tgctcgaagt tgctgtagct
     2941 atcctgaaaa aagaccctta tttcctagtc gttaactgag cagagaaggg agataaaaat
     3001 gaagcaaaca ggcaccaggc aatctagaaa gcattacctc aaactactgc ttgtagctgc
     3061 tgcaattgta gttctcgtaa gttcatcggc ttttgcagca gggattttgc ggcagggcaa
     3121 agctagcaac gatggagcaa agaggatagc attgacgttc gacgatggtc cttatccaga
     3181 gatcacgcct gcgatcttga aagctctaga agaagcagat gttaaggcca cgttttttgt
     3241 tgtaggttgg agggtgtacg aataccacga catctttacc accatgaacg aaaaaggcca
     3301 tgaggtaggc aaccacagct acgaccatcc ccaccttacc agcttgacag cagaagagat
     3361 cgcatcacag ctgaaaaaaa ccaactacgc cgtacagaaa tactctggca aaaaaccggt
     3421 ttatctacga cctccctacg gcagctacaa cgatttggtc ttagagatag cagccgagaa
     3481 tgatatgaag gtagtgcttt ggagtctcaa tcctgaagat tgggcaagtc caggagttga
     3541 cgttatcatc agcagggtct tggagcaggc ctcagacggc tctgtggtgc tcctacacga
     3601 cacatggcag acagctcaga tgctacccga actcatcaca accttgaaag aacgaggcta
     3661 cgtttttgtg actctttcgg agctttttgc agactgaaca ctccttaaaa ccgacaatct
     3721 atatacctga aaacatgcca gcagctaccg ctggcttttt ttgtccccat ttctaactag
     3781 ggaccagagg tagatgaacc aaaagtcctg cgatttgcca tctagatcca aggatttata
     3841 tcgccaggtt tggtacttca cgcatacatt gtaagagtga aggagagaat aactcatagc
     3901 cgcatctggc acggcaggaa ataaagatct ttttaattgc cttttcacga taacacctaa
     3961 gggagggatg tttaattggt gtacggcgaa caagaatctg ttgatgcgct gcagacggca
     4021 ctactaagcc agctggaagt taccgatgaa ggagacctcc actactggtt aggtgaggcg
     4081 ctgctcgaat acagcgacga tccggtagtg atccgggagg gaatctacca tttagttcac
     4141 agcctccgac ttctcagcca gacaagcgag gacaacactt acggcaaagc caagttgaaa
     4201 agaaactgcc tgtctacggc gatgcgcctc aggcagatca gaaacggtcg
//
LOCUS       DAOSDA010000105         4237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13165_length_4237_cov_3.285270, whole genome shotgun sequence.
ACCESSION   DAOSDA010000105 DAOSDA010000000
VERSION     DAOSDA010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13165_length_4237_cov_3.285270"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLW82_03440"
     CDS             <1..500
                     /locus_tag="PLW82_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46359.1"
                     /translation="RDEFGNVRYEWDQRAYRPFWRVTSWNNAPARKEFYWYPGDRVLM
                     RVEVAGAGLLRLTIQDADPNPERVFTTVFEASNFGIGIPTQFKRVNAIDQSGNEGKPV
                     QPTKAKVWGAVWEEVYLLRGDKKYPMVPSRFTDMRCPDGEYFVVVTLNSPGGESISIY
                     GTPEK"
     gene            complement(538..1869)
                     /gene="mgtE"
                     /locus_tag="PLW82_03445"
     CDS             complement(538..1869)
                     /gene="mgtE"
                     /locus_tag="PLW82_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOJ46360.1"
                     /translation="MIEQIIDILESKQYSDIKPLLEGRHPGDISDIIEELPNHYGGIV
                     FRLLPKDLAIEVFENFDSSFRETLLASLKSEEVIKILNEMSPDDRTRLFEELPASVVR
                     KYLALLNDEERKIANMLLGYQDYSAGRIMTTDFLELHEDFTVKRALEAIRSAAPTKET
                     IYYCYVMSRDRKLTGVVSLRTILLSDPEDKVGDLMTAPVISVTTDTPQEEVAATMAEY
                     DFLAIPVVDLEGRLVGIITHDDIIDVITQEDTEDIHLMGGVTTTDKNLMDLTIWDNIK
                     RRAGWLLVMLVFQSFSTSILESYESALSVAVALSFFIPLLIGTGGNSGTQSSTVVIRA
                     IATGELEPKDYWRAIGRELVAASILGGILGVLVSFRAYVSTGSILIGLTVGISLAVVI
                     AIASVAGTVLPMLADRLKIDPAVMAGPLITTVVDIVGLITYLEVAKYILKI"
     gene            complement(2031..3365)
                     /gene="mgtE"
                     /locus_tag="PLW82_03450"
     CDS             complement(2031..3365)
                     /gene="mgtE"
                     /locus_tag="PLW82_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOJ46361.1"
                     /translation="MNQTIYDLVEQKEFGKVKDLLTEMHPKDISTIIADLPEHVQGLV
                     FRLLPKDLAVSVFENFDTSFQEELLQNLKSHEVISILNEMAPDDRTRLFEELPAGVVT
                     KYLAMLTESERKIAYALLGYEEYTAGRLMTTDFLELREDYTISRAIEAIRATAPKKEA
                     IYYGYVISKERELIGVISLRTLISSNPDDTVGELMNTSVICVGTDTPQEDVAAVMAHY
                     DLAAVPVVDTEDRLVGIITHDDIIDVITEEDTEDIHLMGAVTTTDRSFWELTIWDSVK
                     RRLGWLVLLIFLASFSTSILAMYENVLASVVALSFFIPLLIDTGGNAGTQSAAITIRA
                     LATGELKEQDRLEVVGKEVVSSLILGLMVGVVGGFLSYFICGDPSISLAVGVSMVAVV
                     LLASVTGTVLPIIAVALNIDPAVMSGPFITTIVDITGLVTYFYIAKWILNLT"
     gene            complement(3700..>4237)
                     /locus_tag="PLW82_03455"
     CDS             complement(3700..>4237)
                     /locus_tag="PLW82_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46362.1"
                     /translation="PLGVLQAWYYYALENAAPQIAGKWTVAEIPGTIDATGVIDRSSN
                     SNGLSWSIVESSKKKDLAWEFMKFLSSPTFTENFARALYESEGVRIYCATLGFLDQAP
                     FPEKHKDVAQQALLNCRQPTAVVGGYMADRCIDFAFYKVYLEGVDPKEAIMQAAKEST
                     DEIQRKLKEFARFISKIE"
BASE COUNT         1025 a         1049 c         1106 g         1057 t
ORIGIN      
        1 ccagagatga gtttggcaat gtcaggtacg agtgggacca aagggcgtac agaccgtttt
       61 ggcgggttac cagctggaac aacgctcctg ctcgcaaaga gttctactgg tatcctggcg
      121 atagagtgct catgagggtt gaagtagcag gggcaggact cctcagactt acgattcagg
      181 atgccgatcc caaccccgag agagtcttta ccacggtctt tgaagcttcc aacttcggca
      241 tcggcatacc gacccagttc aagcgggtca atgccattga ccagtcgggt aacgaaggca
      301 agcccgtaca gcccacaaaa gcaaaggtct ggggtgccgt ttgggaagaa gtttacctcc
      361 tgcgtggtga caagaagtat ccgatggttc ccagccgctt taccgatatg aggtgtcctg
      421 acggagagta cttcgtagtc gtgactttga acagccctgg cggcgagtcc atttccatct
      481 acggaacgcc agagaagtaa tagagtaaaa aagaacgaga ggaaaagccc tctcgtttta
      541 tattttcagg atatactttg ccacttccag gtaggtgata agacccacga tatccaccac
      601 tgtggtgatc aggggacctg ccattaccgc aggatcgatc tttaagcggt cggctagcat
      661 tggtagaacc gttccggcaa cgctggcaat tgcaattacc accgccaggc tgattcccac
      721 tgtaagaccg atcaagatgc tgccggtgga gacataagcc ctaaagctga cgagaacccc
      781 taagattcca ccaagaatgc tcgctgcaac cagctctctg ccgatagctc gccagtagtc
      841 tttaggctct aactcacctg tggcaattgc cctaataacc acagtagagc tttgggtacc
      901 agagttgcca ccggttccga tcagtagggg aatgaaaaac gacagagcta cagctacaga
      961 gagagcgctt tcgtaggatt ccaggatcga tgtggaaaag ctctggaaaa ccagcatcac
     1021 caggagccag ccggctcttc ttttgatgtt gtcccagatg gtaaggtcca taagattttt
     1081 gtcggtagtg gtaacaccac ccatgaggtg gatgtcttcg gtatcttctt gggtgatgac
     1141 gtcgatgatg tcgtcgtggg taatgattcc caccaggcgg ccttctaggt ccaccacagg
     1201 tatggccaga aagtcgtatt ctgccatagt cgccgcaact tcctcctggg gcgtgtcggt
     1261 ggtaacggag ataacaggag ctgtcatcag gtcaccaact ttatcctcag ggtcgctcag
     1321 gaggatcgtc ctcaaagaca cgacgccggt cagcttccgg tctctactca tgacgtagca
     1381 gtagtagatg gtctctttgg tgggagcggc actgcgaata gcttccaagg cccgcttcac
     1441 cgtgaagtct tcgtggagct ccaggaaatc ggtggtcatg attctgccgg cagagtagtc
     1501 ctggtagccc aaaagcatgt tggcgatctt ccgttcctca tcgtttaaga gggctaggta
     1561 cttgcgaaca accgatgcgg gcagctcttc aaaaagccta gttctatcgt caggcgacat
     1621 ttcgttgagg atcttgataa cttcctcgct tttcaggctc gcaagcaaag tctcccggaa
     1681 ggaggagtcg aagttttcga aaacctcaat cgcaaggtct ttgggaagga gcctgaagac
     1741 gatgcccccg tagtgattgg gcagctcttc gataatatcg ctgatgtcgc cgggatggcg
     1801 accttcaagg agaggtttga tgtcagaata ctgcttgctc tccaaaatgt ctatgatttg
     1861 ctcgatcata tgcttcaccc ctaaaccgta cggccgcctt cagtactgcg aaagaaggta
     1921 accgtactga ttggtattta gttttgatac acgacgggta gggatcacta ctcatcaagg
     1981 ttacactcct ttcaaattct gactcagcct ggttcggcag tgaaagccta ctaggttaag
     2041 tttaaaatcc atttggcaat gtaaaaatag gttacaagac ctgtaatatc aacgatggtg
     2101 gtgatgaaag gtccggacat tacagccgga tcgatgttca gcgcaacggc aatgatggga
     2161 agcactgtgc ctgtaacgct ggcaaggaga accacggcga ccatactcac acctactgct
     2221 agagagatag aaggatcacc gcagatgaag taggagagaa accctccaac tacaccaacc
     2281 atcaaaccta aaatcaaact ggaaaccact tccttgccta ccacttcaag gcggtcctgc
     2341 tctttgagct ctcctgtagc caaagccctg atggtaatgg cagcactttg ggtgccggca
     2401 ttgcctcctg tgtcgattag gagaggtatg aagaaggata gggctacgac agaggcaagg
     2461 acgttctcgt acatggccag gatgctcgtg gaaaagctgg caaggaaaat caggagaaca
     2521 agccagccca acctgcgctt tacgctgtcc caaatcgtga gctcccagaa gctgcggtcg
     2581 gtagtggtta cagcacccat aaggtggata tcttcggtgt cttcttcggt gatgacgtcg
     2641 ataatatcgt cgtgggtgat gattcccacc agccggtctt ctgtgtccac cacaggtaca
     2701 gcagcaaggt cgtagtgggc catgacggca gctacgtctt cttgaggggt atcggttccc
     2761 acgcagatta cagatgtgtt catcaactca ccgacggtgt cgtcgggatt gctggaaatc
     2821 agcgtcctca gtgagatgac tccgatgagc tcgcgttctt tgctgatgac gtagccgtag
     2881 tagatagctt cttttttcgg cgctgtggct ctgatggctt caatggcccg gctgatagta
     2941 tagtcttctc tcagctccag gaaatccgtt gtcattaacc ttcctgcagt atactcctcg
     3001 tatcccagga gggcgtaggc gattttcctc tcgctttctg tcagcattgc cagatacttg
     3061 gtgacaacgc ctgcagggag ctcttcaaag agcctggttc tgtcgtctgg agccatttcg
     3121 ttcagaatgc tgataacctc gtggcttttt aggttttgca ggagctcttc ctgaaaagag
     3181 gtgtcgaagt tctcgaagac ggacacagcg aggtctttgg gcagcaacct gaagaccaat
     3241 ccttgaacgt gttccggaag atcggcaatt atggtactga tgtctttggg atgcatctcg
     3301 gtgagcaagt cctttacttt gccgaactcc ttctgttcaa ctaagtcgta gatagtctgg
     3361 ttcatgggca tcacctcatt tgctttggat gcctcctcgg tgagccgtac cagagggagg
     3421 caaccttttt aatggcgaac acgggtaaga accatctgct gtagcgctac tgtgactatc
     3481 cataggttga atccctccct taactgatga attaagcatt tctgcttgac cttctttatc
     3541 ataacacaga tgcatccagt agagaaacgt ccaggtggcc aattttacct tcactggatg
     3601 cccaaatttc tgccaaagca cttgtcaaaa ccttgtgata atacaataag cccaggtgaa
     3661 acgctggcgt ttctccctgg gctttgcttt ttaatgtcgc tattcgattt tgctgatgaa
     3721 cctggcaaac tctttaagct ttctttggat ctcgtcggta gattcctttg ccgcctgcat
     3781 gatagcctct ttaggatcta cgccctcaag gtagactttg tagaaggcaa agtcaatgca
     3841 gcggtctgcc atgtaaccac ctacaacagc ggtgggctgt ctacagttca gaagggcctg
     3901 ctgggccacg tctttgtgtt tttccggaaa tggcgcttga tccaagaatc ccaaggtcgc
     3961 gcagtagata cggacgcctt ctgattcata gagcgctcga gcgaagtttt cagtaaaggt
     4021 gggagaggaa aggaacttca tgaactccca agccaggtct tttttcttcg acgattctac
     4081 tatggaccaa gacaggccgt tggagttaga actgcggtcg atgacccctg tggcatcaat
     4141 ggtgccgggg atctctgcaa cggtccactt gccggcgatc tgaggtgcgg cattttccaa
     4201 agcgtagtag taccatgcct gcaatacgcc tagggga
//
LOCUS       DAOSDA010000106         4237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13168_length_4237_cov_3.080823, whole genome shotgun sequence.
ACCESSION   DAOSDA010000106 DAOSDA010000000
VERSION     DAOSDA010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13168_length_4237_cov_3.080823"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /locus_tag="PLW82_03460"
     CDS             complement(<1..1664)
                     /locus_tag="PLW82_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulosome anchor protein"
                     /protein_id="HOJ46363.1"
                     /translation="MEKSIAILLCLILIVLPQAFADEKPLAFGQGDGVLKVSNDFIEI
                     AVNASDDGTGRFAVLTTGGDPERIDDDDNFLIYGLGKPVTSYSTIRIGGLDYVFGGKT
                     TKRAGFSGEYGTLVTAPVHRDDGIYTAYRYGDILVEQILSITTSTTTGQPDTAAISYV
                     VTNQGTSPKNVGLRVVLDTMLGNNDGAPFRVRDMAVVGDTMFSGDDIPSFIQAFDDLI
                     RPAVMAQANLAGPEVTKPSRVYFTNWGSVADGVWDFNFQPGRDFTRIGEFDLDSAMAL
                     YWDPTPLAPGESRHYITHYGLGGITVAQGYLSIGLSTVNKVEMAVNPVPFDVIGYIQN
                     TGDGLAHEVIAELQLPKGLKHINEPERFLGRMAPGESRQVVWKVVPTGEAFGSLKITM
                     NVEAVDVDPLSVSRDVDVSKPARLNLSLSGPRNLGIYEEKLYPSIFEVTGTVRNDGDS
                     TAHNVNLRVSTSAFKVVAGANPDFYANYFVGSLAPGEAKEVVWRVDTTPNNMLTYPFG
                     TINVVLAAESTNAEKPADITLAVQVPQVRPKLKLAANAVKRIGDVNV"
     gene            complement(1679..2566)
                     /locus_tag="PLW82_03465"
     CDS             complement(1679..2566)
                     /locus_tag="PLW82_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46364.1"
                     /translation="MALHIFLVLVINAEAFEAMFAGVGNSTGQMIVRVRLEGPDKPAN
                     KGGEKTTEVSQPEKKATGNVENKETQPTNKVVVEPVEKAQTNPPTTQTTATKPPQTTQ
                     QTTTPAPQKVEPPTPKPDPETVQLPKQEVPPAIEVVTSQTSTFEVDLSDMTQEAKGEA
                     APEVEETIQGDAIEKTGSPDGNSDKYVPPKGDDYITRAGGTGGWTTKNIESLEYTGEL
                     EMIFEVDREGKLTVVLVRGMGEERFNRELVSLAERSWKGRFDAEARALFPNGYKVPVI
                     VVFEKGVGTHKFGEVQPLE"
     gene            complement(2636..3034)
                     /locus_tag="PLW82_03470"
     CDS             complement(2636..3034)
                     /locus_tag="PLW82_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOJ46365.1"
                     /translation="MIKYETNKPKIDLTPLIDIMLSLILFFVIFTSFRVYEAAINLQL
                     PKASTGEGALKSSLVISVDQNGDMYLGDNRLSEEQILASVKTRLADNPQEVIIIRAHE
                     DIPYMYVIKAMDIVQAGGGYNISLAVETKQ"
     gene            complement(3031..3675)
                     /locus_tag="PLW82_03475"
     CDS             complement(3031..3675)
                     /locus_tag="PLW82_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOJ46366.1"
                     /translation="MLELLAKGGWVIYVLLACSVVALAIALERLYNLLRAQNDADKLI
                     GAVKRHLNQGRFLEALKFAKQERGPIAAVLAAGIQNYGRDLNEVERNMNLVAEQELYR
                     MRKRLPALQIIATISPLLGLLGTVTGEIKVFNVLGQLQGVSDPVAMSSGIAEALLTTA
                     YGLIIAIPVSVIYSYLDSLVVKNVTSMNRAMAEILDIFANLKVETSEKETSDIA"
BASE COUNT          986 a         1102 c          989 g         1160 t
ORIGIN      
        1 acgttgacat cacctattct tttcacagca ttggcagcaa gtttcagctt cggccttacc
       61 tgaggcacct gtaccgctag agttatatct gccggttttt cggcatttgt tgattccgct
      121 gccaaaacca cgtttattgt tccaaaagga taggtcagca tgttgttagg tgtggtgtca
      181 actcgccaaa ccacttcctt cgcttctcca ggagcaaggg agccgacaaa gtagttggca
      241 tagaagtcgg gatttgctcc agcaactact ttaaaggccg acgtggacac cctgagattg
      301 acgttatgag cagttgagtc gccgtcgttt cttacggtgc ctgttacttc aaagatagaa
      361 ggataaagct tttcttcgta gattcccagg tttctcggcc cgctcaaact gaggttgagc
      421 cttgctggct tgctgacatc aacatcccta gaaactgaca gaggatctac gtcaactgct
      481 tccacattca tggttatctt caaactgcca aaagcttcac ctgtaggtac gaccttccag
      541 accacctggc gggactctcc aggagccatc ctgcccagga acctttcagg ttcattgatg
      601 tgcttcaagc ctttaggcag ctgcagctcg gctattactt cgtgtgcaag accatcaccg
      661 gtgttctgga tgtagcctat tacgtcaaac ggcaccgggt taaccgccat ttccactttg
      721 ttgaccgtgc taaggccgat ggagaggtaa ccctgagcaa cggtgatgcc gcctagaccg
      781 taatgggtga tgtagtgcct cgactcgcct ggcgctagcg gagttggatc ccagtacagt
      841 gccatggcac tgtcgaggtc gaactcaccg atgcgggtaa agtcgcgacc cggctggaag
      901 ttaaaatccc acacaccgtc tgccacacta ccccagttgg taaagtatac tctactgggc
      961 tttgtgacct caggtccagc aaggttagct tgagccataa ctgcaggcct gatcaggtcg
     1021 tcgaaagcct gtataaacga agggatatcg tcacctgaga acatggtatc gccaacaact
     1081 gccatatccc gcactctaaa gggggcaccg tcgttgttgc caagcatggt gtctagaacc
     1141 acgcggagac ccacattctt tggcgacgta ccttggttcg tcaccacgta actgatggcc
     1201 gcagtgtcgg gctgacctgt tgtggttgat gtagtgattg aaagaatctg ttcaaccaaa
     1261 atgtcgccgt acctgtaggc cgtatatatt ccatcatctc tgtgcactgg ggccgtgacc
     1321 agcgtgccgt attcaccgct gaatcccgcc ctcttagtgg tttttcctcc gaaaacgtag
     1381 tcaagaccac cgatacggat ggtgctgtat gatgtaaccg gtttgcccaa accgtagatc
     1441 aggaagttat cgtcatcatc gattctttcc ggatcgccac cggttgtcag aactgcaaat
     1501 cggcccgtgc catcgtcgct ggcgttgacc gcaatttcaa tgaaatcatt acttactttg
     1561 agtaccccgt caccttgacc gaaggcaaga ggtttttcat ctgcaaaagc ctgtggtaga
     1621 acaatgagga tcagacatag caagattgcg attgattttt ccacatctct tacctcctct
     1681 actctagcgg ttgaacttca ccaaacttgt gggtacctac gcctttttca aacacaacaa
     1741 ttaccgggac cttgtagccg ttggggaata gagctctagc ttctgcgtca aagcgccctt
     1801 tccacgaccg ctctgcaaga ctaaccagtt ctctgttgaa gcgttcttca cccatacctc
     1861 gaaccagaac cactgtgagc ttaccctctc tgtctacctc aaagatcatc tctagttcgc
     1921 ctgtgtattc aaggctttcg atgttctttg tggtccagcc tccggtaccg ccggctctag
     1981 tgatgtagtc gtcgcctttc ggtggaacgt acttatcgct gttgccgtcc ggagaaccgg
     2041 tcttctcgat ggcgtcgccc tggatggttt cctcaacctc tggagcagct tcacccttag
     2101 cttcctgcgt catatcgctc aaatccactt caaaggtact ggtttggctt gtaacaactt
     2161 cgatcgcagg aggcacttct tgctttggca gctgcaccgt ttctggatcc ggcttaggag
     2221 taggaggttc gactttctgc ggtgcaggtg tagttgtctg ctgggtcgtt tgaggcggct
     2281 ttgtagctgt ggtttgagtg gtaggaggat tcgtctgggc tttttcaact ggctccacca
     2341 ccaccttgtt ggttggttgc gtctccttat tctccacatt tcccgtagcc tttttctcag
     2401 gctgagatac ttcagttgtc ttttctccgc ctttgtttgc aggtttatcg ggaccctcca
     2461 ggcgtactcg aacgatcatc tgtccggtgc tgtttcctac acctgcaaac attgcctcga
     2521 acgcttcggc gttaattact aaaaccagaa aaatgtgtaa cgccaaggaa ataacgaaaa
     2581 agacagagag ggccttattg tcattatgtg cttgcattta agcccactcc ttttgtcatt
     2641 gttttgtttc gacagccaag gagatgttgt aaccgcctcc ggcctgtaca atgtccattg
     2701 ctttgattac gtacatgtaa ggtatatctt catgtgccct aataatgatt acttcttgag
     2761 gattgtcggc aagcctggtc ttaacggaag ccaggatctg ctcttcgctt aggcggttgt
     2821 cacctaggta catgtcgccg ttttgatcga cgctgataac cagcgagctt ttcagcgcac
     2881 cttcaccagt agaagctttg gggagctgaa ggttgatggc agcttcgtat accctgaagc
     2941 tggtaaaaat gacgaaaaat agaatcagag acagcatgat atcgatcaga ggagtcagat
     3001 cgatcttcgg cttattggtt tcgtacttaa tcaagctata tcactcgttt ccttttccga
     3061 cgtttcaacc ttcaggttgg cgaagatatc gagaatctcg gccatagccc tgttcatact
     3121 ggtcacgttc ttaactacta aagagtccag atatgagtat atcaccgaaa cgggaatagc
     3181 gataattaaa ccgtaggctg tagtcagaag agcttcggca ataccagagc tcatggcaac
     3241 agggtcggaa acgccctgga gctgtcctaa aacgttaaaa accttgatct cgccggtaac
     3301 tgtacctaaa aggccgagca gcggcgaaat tgttgcaata atctgcaggg ccggcagtct
     3361 ttttctcatt ctatagagtt cttgttctgc aacaagattc atattgcgct caacttcatt
     3421 gaggtcccgt ccgtagttct gaatgcccgc agctaaaaca gcagcaatcg gacctctttc
     3481 ctgcttggca aacttcaaag cttcgagaaa tctgccctgg ttaagatggc gttttactgc
     3541 accaatgagt ttgtccgcat cgttttgtgc gcgaagtaga ttgtagagac gttctagggc
     3601 aatggccagt gctactacag aacacgccaa tagcacatat attacccatc cacctttagc
     3661 taatagttca agcataatcc gcctgccaaa aagcaggctt gccacctcct tgagagctgt
     3721 ttatcttcgt cttgcgattc cggtctagag tactgctaca gcagctgtca attctcatga
     3781 ctgctttcga caaagccgca gtgttttcct tgtacgccag ctaaaggaac cgttagaatc
     3841 ttttcacatt cacggcgtta aaattcctcc accagtcaag ctaccccgag aggtcctctt
     3901 gcagctacga gggaaaactg ctgtccatta caagttctgt tcggccgggc cttttgcttt
     3961 aatgtttaca aagctgctac cggcgattct tccagtcagc gaacataaga agaaaggcca
     4021 caaagatccc aacccgaaag agccagatct tttgcctacc gaaaaaaccc tgatgcttat
     4081 tcagactcat ttagaccgtc gcagttacgc aaaagaggaa aaaaatcggg tgtttctagt
     4141 gcctgcaggt gtttctctcc gagcccgttt tctttcgaac gcaaaagaag actccggcgg
     4201 ccttccggcc cgactgcgga gccttctttg tatcctg
//
LOCUS       DAOSDA010000107         4230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13198_length_4230_cov_3.074970, whole genome shotgun sequence.
ACCESSION   DAOSDA010000107 DAOSDA010000000
VERSION     DAOSDA010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13198_length_4230_cov_3.074970"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLW82_03480"
     CDS             <1..257
                     /locus_tag="PLW82_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46367.1"
                     /translation="WGVLGENYYGLITTAEVSPENLDLSGITGDVPSRDAILKDLLLL
                     KKPKFFKKCRSAARSLVERFYSLDQMVERTLEVYSKVLAG"
     gene            396..743
                     /locus_tag="PLW82_03485"
     CDS             396..743
                     /locus_tag="PLW82_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46368.1"
                     /translation="MGIKVKCCPPSDGISVTSEVGENSVITIPVGAPGARVHITQIWA
                     ANAPSADPGKLLTIESEGGTVLWQEFTETDPFISRYFNSPLRGPIGEDVTITFEGHPG
                     STSTLSVTYFISV"
     gene            928..1941
                     /locus_tag="PLW82_03490"
     CDS             928..1941
                     /locus_tag="PLW82_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46369.1"
                     /translation="MRKIGSEISAGPVLGIMARGLDDESAERFIRYFMKGSVLSYVFD
                     ESQLTKQQINGYYFDEEWHHLKLPKPNFVFDRTYPSRPENRSRLPRSVFINPITQFSK
                     SQVAKILAQNGIAVPETFSGTFEKAYQMFSLFYLKPDRGSFGQDMFIVQKQSEDYTVY
                     TSTSDTLHTYSANEIEWLLGAIQENGYILQRGIEPIYYKGEPADFRLHLLADPHPRVT
                     LIAARTSRWPFLYTSTPKRIIKTGRVLSEKDKKALEDLAIKVHSVISKVYGPYVELSL
                     DVVQEKSSEKFYVIDVNGKSTRVHPQILRYDIEEYFRAPEKLANHLFVTGGDLFMGMG
                     RVR"
     misc_binding    2178..2392
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2426..>4230
                     /locus_tag="PLW82_03495"
     CDS             2426..>4230
                     /locus_tag="PLW82_03495"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOJ46370.1"
                     /translation="MSEKNLSTVYDPKQVEDRLYDFWVKNNYFHGEVDNTGEKEPFCI
                     VIPPPNVTSHLHIGHALDNTLQDILVRFNRMKGKNTAWIPGTDHAGIATQIKVEEALR
                     EKENLTRHDLGREEFLKRVWQWKEKYGSTIINQLKKLGSSCDWERERFTMDEGCSKAV
                     REVFVSLYEKGLIYQGDYIINWCPSCNTALSDVEVEHTDMNGHFWHIRYPLADGSGYI
                     EIATTRPETMLGDTAVAVHPDDERYAALVGKEVILPLVGRKIPIVADEYVDPEFGTGV
                     VKITPGHDPNDFEVGTRHNLPIETVIGFDAVMTEAAGKYQGLDRYEARKRVVADLEAG
                     GYLVKVEDTTHAVSTCYRCDTVIEPLVSKQWFVKMAPLAKPAIEAVKDGRIKFYPERF
                     AKLYLNWVENVRDWCISRQLWWGHRIPVWYCDDCGEVIVPRENPTHCPKCGSEKLTQD
                     PDVLDTWFSSALWPFSTMGFPEKTAELDYFYPTSVLVTGFDIIYFWVARMVFMGLEFM
                     GDVPFRDVYIHGLVRDHLGRKMSKSLGNGIDPLEVIDQFGADALRFTLVNGVAPGNDM
                     RFHMEKVEASRNFMNKIWNASRFALMHLEGKVPEK"
BASE COUNT         1125 a         1029 c         1085 g          991 t
ORIGIN      
        1 cctggggcgt tttgggagag aactactacg gtctaatcac caccgctgaa gtatccccgg
       61 aaaaccttga tctaagcggt ataacgggag atgtgcccag cagagatgca atcttgaagg
      121 accttctcct cttaaaaaaa cctaagttct ttaaaaagtg ccgtagtgca gccaggtccc
      181 ttgtggaaag gttttactcc cttgatcaaa tggttgagcg caccttggag gtctactcca
      241 aggtccttgc aggatgatca ccactttgag caagacttag gagagttttt gcctgtgcta
      301 aaaaccccaa gtaacctaca tttgagttga acctatctac ccgcagcaaa actttccttc
      361 gggcatattt tagctttgaa aggaggtaga aagctttggg tattaaagtg aaatgctgcc
      421 cacctagcga cggcatctca gtcaccagtg aggtcggcga aaactccgtg atcaccatcc
      481 ccgttggcgc tcctggagcc agagtccaca ttacgcaaat ctgggcagca aacgcacctt
      541 ctgccgaccc agggaaactc ctcaccatcg agtctgaagg tggaactgtg ctctggcagg
      601 agttcacaga aaccgatcct ttcattagcc ggtacttcaa cagccccctg agagggccga
      661 taggcgagga tgtcaccatt actttcgagg gtcatccggg atctacaagc acactttcag
      721 ttacctactt cataagcgtt taacatgcag atggtacggg gcagatggac aaaaaaccag
      781 attgagctga atcgtacttt ccttacaagg ttccctgata agtttgaaat ctacctggga
      841 gccgagaagg tacaggtggt aaataagaag agttgtggta tcttaatcgt caaatcgttt
      901 tatcttccta gttcacgaga aataactgtg agaaagatag gaagcgaaat ctctgcagga
      961 ccggtattgg ggatcatggc aagaggtttg gacgacgaaa gcgccgagag gttcatccgg
     1021 tactttatga aggggtcggt tttaagctac gtttttgacg aaagccagtt gacaaaacag
     1081 cagattaatg gctactactt cgatgaagaa tggcaccacc tcaagctgcc aaagccgaac
     1141 ttcgtcttcg atcgcaccta tcccagccgg ccggaaaaca gaagcaggct gccccgatcg
     1201 gtgttcatta atccgataac tcagttttcc aaaagccagg tggcaaaaat actggctcaa
     1261 aatggcatcg ctgtgccgga gactttctcc ggcacatttg aaaaggccta tcagatgttt
     1321 agcctgttct acctaaaacc ggaccggggc tctttcggcc aggatatgtt tatcgtacaa
     1381 aagcagagcg aggactacac tgtctacaca tcgacctcgg acaccttgca tacctactct
     1441 gcgaatgaga tagagtggct tttaggagcc atccaagaaa acggctatat tctgcagcgg
     1501 gggatcgagc ctatctacta caaaggcgag cctgcagact tccgcctgca ccttctagca
     1561 gatccccacc cgcgggtgac tctcattgcg gcacgaacgt ccagatggcc tttcctgtat
     1621 accagcacgc cgaagcgcat catcaagacc gggcgggtcc tcagcgaaaa agacaaaaag
     1681 gctttggaag atttggcaat caaggtccac tcagtgattt ccaaagtata cggtccttat
     1741 gtggagcttt ctctagatgt ggtccaggag aaatccagcg aaaagtttta tgtaattgat
     1801 gttaacggta agtccacaag ggtccatccc cagattctgc gctacgatat tgaagagtac
     1861 ttcagagcac cggaaaagct tgccaatcac ctctttgtta cgggaggaga cctttttatg
     1921 gggatgggaa gagtaaggta acctttttcc ctccgcgagg tccaataagg cacatgaaaa
     1981 ccacctcctt tctgattgac tctttaaaaa tacgtttata ctccttccaa aaagcttacc
     2041 ttgcatggta agcttttccc gtgtgtccac atgtgttagg ggtctgtaag acctgcagca
     2101 gggctttttt ccttaaggtt ttacgctgaa aaaagtttga catttagggt gcgatgggct
     2161 atatttttaa taaaataaga taggctatga agaggaggag taacaagcct agctttctca
     2221 gagaggcagc gtttggtgga agctgctaaa gcgaagtttg tgaaggtcgc ctcggagctt
     2281 tgccggtgag gtttgtaaat ggcttagtcg gcaacgagta atgcccgtta acgcatgaga
     2341 gtgcttcctt tcaggaagaa ttaaggtggt accacggcct tccgtcctta tggattggaa
     2401 ggttttttta ttaggaggta ttgtgatgag cgagaaaaat ctcagtacag tctacgatcc
     2461 caaacaggtt gaggaccgtt tgtacgattt ttgggtgaaa aacaactatt tccacggcga
     2521 agtagataac acaggagaaa aggaaccgtt ctgcattgtt atcccgccgc caaacgtcac
     2581 ctcacatctg cacatcggcc acgctctcga caacactctg caggatatcc tcgttcgctt
     2641 taaccgtatg aaggggaaga acactgcctg gatacctggc acagaccacg ccggtattgc
     2701 gacccagatc aaagttgaag aggctctgcg ggaaaaagag aacctcaccc gtcacgatct
     2761 cgggagagaa gagttcctga aaagagtctg gcagtggaag gaaaagtacg gcagcaccat
     2821 catcaatcag ctgaagaaac tcggctcatc ctgcgattgg gaaagagaac gcttcactat
     2881 ggatgaaggc tgctcgaaag ctgtaagaga ggtcttcgtc agcctttacg aaaaaggcct
     2941 catctaccaa ggcgactaca tcatcaactg gtgtcctagc tgcaacacgg cactgagcga
     3001 cgtcgaggta gagcatactg acatgaacgg tcacttctgg catatccgct atccccttgc
     3061 agatggctcg gggtacatcg agatcgctac aaccaggcct gagaccatgc tcggagacac
     3121 tgccgttgca gttcaccctg acgatgaacg ctacgctgcc ctagtaggta aagaggtcat
     3181 tcttcctctg gttggcagaa agatcccaat tgtcgccgac gagtacgtgg atcctgagtt
     3241 tggcaccggc gttgtgaaaa tcacaccggg tcacgacccc aacgacttcg aagttggcac
     3301 caggcataac ctgcccatcg aaactgtgat cggttttgat gcggtgatga cggaagctgc
     3361 cgggaagtat caggggcttg acagatatga agctagaaag agagtggtag ccgatcttga
     3421 agccggaggg tatctggtca aggtagagga caccacccat gcggtaagca cctgctaccg
     3481 ctgcgatacg gttatcgaac ctctggtcag caagcagtgg ttcgtcaaga tggcacctct
     3541 ggcaaagccc gccatcgaag cagttaaaga cggaagaatc aagttctacc ccgagcgctt
     3601 cgccaaactc tacctcaatt gggttgaaaa cgtcagagac tggtgcatca gcaggcagct
     3661 ctggtgggga cacagaatcc cagtatggta ctgcgacgac tgcggtgagg tcatcgttcc
     3721 tagagagaac cccacccact gtccgaagtg cggttcggag aaactcactc aggatcccga
     3781 tgttcttgac acttggttca gctcggctct gtggccattt tccaccatgg gcttcccgga
     3841 gaaaaccgcc gaactggact acttctatcc cacatcggtt ctggtgacag gcttcgatat
     3901 catctacttc tgggtagcca gaatggtgtt catgggcctt gaattcatgg gcgatgtgcc
     3961 ctttagggat gtctacatcc acggtctggt ccgcgaccac ctggggcgga agatgtcgaa
     4021 gtctttggga aatggcatcg atccgttgga ggtcattgac cagttcggag cagatgcgct
     4081 gagatttacc ctggtcaacg gcgttgcacc aggtaacgat atgcgcttcc acatggagaa
     4141 ggttgaagcc agccgcaact tcatgaacaa gatctggaat gcatcgaggt ttgccctcat
     4201 gcacctggaa ggcaaagttc ccgagaagaa
//
LOCUS       DAOSDA010000108         4225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13216_length_4225_cov_3.262350, whole genome shotgun sequence.
ACCESSION   DAOSDA010000108 DAOSDA010000000
VERSION     DAOSDA010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13216_length_4225_cov_3.262350"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLW82_03500"
     CDS             <1..477
                     /locus_tag="PLW82_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOJ46371.1"
                     /translation="VGKSTVINALAKSAKARTGEKPGVTRGKQWISIKNNWQLLDTPG
                     VMVPEALEGERALFLALINAVDERLYDLELAALHFLAWLKENAPAALTSRYKLKEDLV
                     EPHELLEEIGRKRGCLRSGGVVDLTQAAFVLLKDFREGGLGKLSLETPESGGNGDD"
     gene            470..1258
                     /locus_tag="PLW82_03505"
     CDS             470..1258
                     /locus_tag="PLW82_03505"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOJ46372.1"
                     /translation="MIDFTAMSLKQIKEKLEEYGPYVPEELLEALRKDGRAGAKKLAA
                     AIERQNQEKDNVLQHFRELGDFDRKYGCRVVGIDEAGRGPLAGPVVAAAVELDPDHID
                     WAVGIDDSKKLTREKRREICATIKAKALFCGIGVVEASVIDQMNILNATHLAMAKAIK
                     DAKEGFLCLVDGLPVPRLGRRQEAIVKGDAKSLSIAAASIVAKETRDSLMDHWDAVYP
                     EYGFAKHKGYGTKEHYIALERYGPCPIHRKSFLTGFELRQGVLF"
     gene            1486..2529
                     /locus_tag="PLW82_03510"
     CDS             1486..2529
                     /locus_tag="PLW82_03510"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOJ46373.1"
                     /translation="MQLLRIHQLTEGMVLAKDVYDYSGRVLLFEGIELKNSFIARLKE
                     LDVYALYVQAKGDVSPFDKSIKPISREETLKAITDSIQDVTAAKSIDVSQIKEDILNL
                     LQTITQHKTVCLCISEIQNYDSYTESHSFNVAVLSLVIGIKLGLSESELLDLRMGAIV
                     HDIGKLYIQRKLLNKAEKLTNVDYTELKRHTQYGHDMLANVDGISQEVRDIILDHHER
                     MNGSGYPRGLKRDEIGLFSKIVMVADAFDAMTSDRIYKKGVTPYEAVRVIKAMGGVLF
                     DPDVVEAFLSTVVPYPAGSMVELSNGQIGVVTASDPDLTVRVIYDEQGYKVNEAKEFV
                     LDEDTELKVIRIS"
     gene            3271..>4225
                     /locus_tag="PLW82_03515"
     CDS             3271..>4225
                     /locus_tag="PLW82_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ46374.1"
                     /translation="MKSKGLLVAIAILAIAVVVYFAFFTGKGEEESDVIKIGFIGPLT
                     GPAASYGINARNGALIAVEQINAAGGVLDGKKLQLIVEDDKGDTTEATNVFNKLVEQD
                     KVVAIVGPVTTGPTTVVARLAGEERIPIVSPTATGDDITKVSDFVARVCFYDSYQAPV
                     MAVFAVEKLGYTRAAILYDNSNDYSIGLANAFALRFTELGGTIVAKEAFTEGDQDFNA
                     QLTNIAAANPEVVYVPAYYSDDAQILLQARQLGMTAVFMGGDGWDSDELVKAAGSAAE
                     GSYFTTHYSSSDESEQVLEFLKTYKQKYNTEPNFAGPLGYDA"
BASE COUNT         1196 a          893 c         1083 g         1053 t
ORIGIN      
        1 gttggcaagt ccaccgtcat taacgccttg gcaaagtccg ccaaagccag aactggcgaa
       61 aaacccgggg taacccgagg caagcaatgg atatctataa agaataactg gcagcttttg
      121 gacacgcctg gtgttatggt gcccgaagcg ctagagggag agcgggcgtt gttcctggcg
      181 ctgatcaacg cagttgatga aagactctac gatttggaac ttgcagccct gcactttttg
      241 gcttggctga aagagaacgc tcctgcagct ttaacctcca ggtataagct gaaagaggat
      301 ttggtagaac ctcatgaact cttggaagag atcggcagaa aacgaggctg cttgcgaagc
      361 ggtggagtgg ttgacctgac tcaagctgca tttgtgcttc tcaaagactt ccgcgagggt
      421 ggtttaggaa agctttctct ggagacgccc gaatctggag gaaacggcga tgattgactt
      481 tacagcgatg agtcttaagc agatcaaaga aaagctggaa gaatacggac cgtatgtccc
      541 tgaagaactc cttgaagccc tacgcaaaga cggccgtgca ggcgcgaaga aactggccgc
      601 ggcaatcgaa cggcagaatc aggagaaaga caacgttctg cagcactttc gagagcttgg
      661 agactttgac aggaagtacg gctgcagggt ggtaggcatc gatgaagcgg gaaggggccc
      721 tcttgcaggg ccggtggtcg cggcagcggt agaactcgat ccggatcaca ttgactgggc
      781 cgttggaatc gacgactcta agaagctaac cagagaaaaa cgcagagaga tctgcgctac
      841 cataaaagca aaagcccttt tttgcggcat aggcgtagtg gaagcatctg taatcgatca
      901 gatgaacatt ttaaacgcaa cgcatcttgc catggcaaag gccatcaaag atgcaaagga
      961 aggctttttg tgcctcgttg acggtctgcc tgtgccgagg ctcggcagaa ggcaggaggc
     1021 tatcgttaaa ggcgatgcaa agtccctttc gatcgcggct gcctcaatag tcgctaaaga
     1081 aaccagggat agccttatgg atcactggga cgctgtttat cctgaatacg gttttgccaa
     1141 acacaaaggt tacggaacta aggagcacta catcgctctg gaaagatacg gtccgtgccc
     1201 catccaccgc aagagctttc ttacaggttt cgaactccgt cagggagtgc tgttttaatt
     1261 gcatattata ccgtaggttt tgcaaattaa taggtaaaaa gtcctttcct atcgtggttg
     1321 taaaggaatc ttaaggtcaa ctggaaaata ttactatgaa agatgtgaaa catcttttgt
     1381 aagacaaaaa cctgtgggca atatcggctt gatttgtcct tattttcacc agcacatgct
     1441 gtactcgata agtcctcgaa aaccgtttga aagaggtgct aacttatgca gttattaagg
     1501 atacaccaat taactgaagg catggttctt gcaaaagatg tatacgatta ttcaggtcga
     1561 gtcctattgt ttgaaggcat tgagctgaaa aacagcttta tcgccaggct gaaggaactg
     1621 gacgtatatg ctctgtacgt ccaagccaaa ggagatgtat ctccttttga taagtcgata
     1681 aagccgatca gtagagaaga aactctgaaa gccatcacgg actcgatcca agacgtgact
     1741 gctgcaaaaa gcatcgatgt cagtcagatc aaagaggaca ttttgaatct tcttcagacc
     1801 atcactcaac ataaaacagt ctgcctttgc atctcggaga tccaaaacta cgacagctac
     1861 actgaaagcc acagcttcaa cgtggcagtg ctgagccttg taattggcat taagctcggg
     1921 ctttcagaaa gcgagctttt ggatctgcgc atgggcgcca tcgtccacga cataggaaag
     1981 ctttacatcc agagaaagct tttgaataaa gcggaaaaac tgacaaatgt cgactatact
     2041 gaactaaaac gccacaccca gtacgggcac gacatgctcg ctaacgttga cgggatctct
     2101 caggaagtaa gggacattat cttagaccat catgagagaa tgaatggatc gggatacccc
     2161 aggggattga agcgggatga aatcggtctc ttctcgaaaa tcgttatggt ggctgatgcc
     2221 tttgatgcca tgacttcaga ccgtatctac aaaaagggtg taacgcccta tgaagcggtt
     2281 agggtgatta aagccatggg tggagtcctg tttgacccag atgttgtaga ggcctttctc
     2341 tctacggtgg tgccctatcc tgcaggcagc atggtcgagc tcagcaacgg ccaaattggc
     2401 gtggttacag ccagtgatcc ggatttaact gtaagagtga tctatgacga gcaggggtat
     2461 aaagttaacg aagcaaagga gttcgttctt gacgaagata ccgagctcaa ggttattaga
     2521 atttcctaaa ttggttttag aaggtaacgg cttctcgaaa gggcgttacc ttttttgcat
     2581 ggtagaacag cggatctgct ttttgttggg actaaaagaa gaactgttga ttatctggga
     2641 gtcgatgaca gtactaaaga tagttcgtaa actgtatatg caggtgtttg aaacgagtgt
     2701 agctttacat aatcgattgc atcttttgtc tgatgggaag gaaaacgaaa gacttgggag
     2761 agtacatatg atccataacg aaacgtccac aaggtgttga catttctggg cgtttttttg
     2821 gtgtaaaaca ccaaaggtct tttcacctga ggttagggtc ctaaaatttc aacatttcca
     2881 actcctccaa aacacagcca ggactcccca acgatgctat tgtgagttgt ggtttgtagt
     2941 gttaaacgct accgttacaa ttagcaagac ttcctgttac tacatgcttt ttcgaggttg
     3001 aagtcgcttt tgtgattgcg gacaggtcct ggagatgatt gataggtaaa aaactcttga
     3061 atcccacttc aaaaggtgat acaaatatta gaaagaccaa gtttttcctt catctgcccg
     3121 ggcattttgg acgaatagcg tacttatagt gacgttttgt tacttatttc ttttcaaaga
     3181 gctgttaatg tctggaaaaa atgtcagaat gtggcaggga tggtaaagcc cacgtcgaat
     3241 aacacgagaa ataacaaggg aggaaaagaa atgaagagta agggactttt ggttgcaatt
     3301 gcgattttgg cgattgcagt tgtagtttac tttgccttct ttacaggcaa aggcgaagag
     3361 gaatccgatg tcattaaaat cgggtttatt gggcctttga ccgggcctgc ggcttcatac
     3421 ggaatcaacg ccaggaacgg tgcattgatc gcggtagaac agatcaatgc cgcaggtggg
     3481 gttttggatg gcaaaaaact gcagctcatc gtggaagatg acaaaggaga taccacagaa
     3541 gctactaacg tgttcaacaa gttggttgag caggataagg ttgtggccat cgttggtcct
     3601 gttactaccg ggcctacaac tgttgtggca agacttgccg gagaagagag aattcctatc
     3661 gtttcgccaa ctgcaaccgg cgacgacatt actaaagtca gcgatttcgt agcccgtgtt
     3721 tgcttctacg actcctacca agctcctgta atggcagtgt ttgcagtcga aaagctaggc
     3781 tacaccagag cagcgattct gtacgacaat tccaatgact attcaattgg attggccaat
     3841 gcgtttgctc tgagatttac agaacttggc ggaactatcg ttgccaagga agcgtttacc
     3901 gagggagatc aggacttcaa tgctcagctt accaacatcg ctgctgcaaa tcctgaagtc
     3961 gtctatgtac ccgcctacta cagcgacgat gctcagattc tcctgcaggc aagacagttg
     4021 ggtatgactg cagtcttcat gggcggagac ggttgggact ctgatgaact ggttaaagcc
     4081 gctggcagtg ctgctgaagg ctcttacttc acaactcact attcgtcaag cgacgagtcc
     4141 gaacaggttc tcgagttctt gaaaacttac aaacagaaat acaatacaga acccaacttt
     4201 gcaggtccgc taggctatga cgcag
//
LOCUS       DAOSDA010000109         4191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13358_length_4191_cov_2.627660, whole genome shotgun sequence.
ACCESSION   DAOSDA010000109 DAOSDA010000000
VERSION     DAOSDA010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13358_length_4191_cov_2.627660"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /gene="dapF"
                     /locus_tag="PLW82_03520"
     CDS             <1..640
                     /gene="dapF"
                     /locus_tag="PLW82_03520"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOJ46375.1"
                     /translation="SEGKMCGNAIRCVGKYLYDNKLVQKLELKIETLSGIKHLKLFAE
                     NGLVYKVTVDMGRAELDAEKIPVTLPSSKVVNEPVTIGGENYRITCVSMGNPHCVVFG
                     SDPEGLDLEKIGPQFERNPIFPERVNTEFVQVLDESTLKMRVWERGSGETLACGTGAC
                     ASAVAAVLNGYCRENTDIKVILRGGELVINYSPERVLMTGAAVKVFDGVVSV"
     gene            677..1408
                     /gene="nadE"
                     /locus_tag="PLW82_03525"
     CDS             677..1408
                     /gene="nadE"
                     /locus_tag="PLW82_03525"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOJ46376.1"
                     /translation="MSRDYEIELENRVAFIKRVLAESGASGIVYGNSGGKDSALVGIL
                     CKKACEDTLGLIMPCATKRNYVEDMEDALTVAKEYGIKTEVVDLTPLKKLFVETLGER
                     FSLQEVALHNLAPRLRMTTLYAVAASQNRLVAGTGNRSEGYVGYFTKWGDGAYDFNPI
                     ADLTVTEVYQFLDFLGAPECIMKKAPSAGLFDGQTDEGEMGLTYKELDSLLLGGTVRS
                     EVLEAIERLHKRSEHKRRPPLVYGG"
     gene            1411..2589
                     /locus_tag="PLW82_03530"
     CDS             1411..2589
                     /locus_tag="PLW82_03530"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOJ46377.1"
                     /translation="MKPNTNYLNLKDSYLFSTVARKVAEYKSKNPGADIIRLGIGDVT
                     LPLPGVVVEAMGKAVLEMGDKKTFRGYGDEQGYVFLREAILGYYSRKNVSLELDEVFI
                     SDGAKSDLGNILDIFSVDNTVVIPDPVYPVYVDTNTMAGRSIVFLDANLDNGFLPLPD
                     DSLQADIIYLCSPNNPTGAVYTKEMLQKWVDFALERNAVILFDAAYEAYVRDPSLPTS
                     IYQIEGAKACAIEFCSLSKTAGFTGTRCGYTVVPKELVFDGVSLNKLWLRRQTTKFNG
                     VPYIVQRGAEAVFTERGLQEVGECISYYLENAKIIRETLESMGIEFTGGANSPYIWLK
                     CPGGMSSWEFFDYLLEKANVVGTPGVGFGKNGEGHFRLTAFGDRENVLTAVGRLKSLI
                     "
     gene            2835..>4191
                     /locus_tag="PLW82_03535"
     CDS             2835..>4191
                     /locus_tag="PLW82_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOJ46378.1"
                     /translation="MTEYFAHSAGKSGRKEYVIEHLTEVAKHAQVFAAPWGAESEAYV
                     AALAHDLGKYSQRFQQVLEKQATRIDHATPGAKVLLEKYKNAGFASALAVEGHHEGIK
                     RGSPKELLDRIRMDKGFSETQSEYSERDIQSLIERLLRDGGILPDHIESSYLDVHKDP
                     VRAQLYVRMLFSALTDADFLATEAHFNRTNDEYEYRKMQNDLNEAMIDKVLDRLLSHI
                     EKIKKEKKSSSEMQSLRDDLLENCLKKASLPTGVFTLTAPTGSGKTFSMLAFALTHAK
                     EHRLRRLIFVLPYINIIDQMAKQFKDVVNQVDEGVFVLEDHSLVEIASDSDELKYLAE
                     NWDAPIIITTSVRFFESLFANRSNDCRKLHNIAKSVILFDEAQTMPSHLAPPSLIALS
                     ELVDRYGCTVVFSTATQPAFTSLNPEIAKLSANTVWKPTEIVDPELKLFQRSKKVDVE
                     WI"
BASE COUNT         1152 a          887 c         1127 g         1025 t
ORIGIN      
        1 aagcgaaggc aaaatgtgcg gaaacgccat tcgctgcgtg ggtaagtact tatacgacaa
       61 caagcttgtt cagaagttgg agctgaagat agagacttta agcgggataa agcacctcaa
      121 actgtttgca gaaaacggtt tggtgtataa agtcaccgta gatatgggaa gggcagagct
      181 ggatgcggaa aagattcccg ttactctgcc aagttctaag gtggtcaatg aaccggtcac
      241 aatcggcggt gaaaactacc gcattacctg cgtctccatg ggcaaccctc actgcgttgt
      301 cttcggcagt gatccggaag gtttggatct ggaaaagatc ggccctcagt ttgaacgaaa
      361 tccgatcttc cctgagagag taaacaccga atttgttcag gtgctcgatg aaagcaccct
      421 gaagatgcgg gtttgggaaa ggggcagcgg agagaccctt gcctgcggca cgggagcctg
      481 cgcgtctgca gtggcggcag tccttaacgg ctattgccgg gagaacaccg acattaaggt
      541 gattctgcga ggcggagagt tggtaatcaa ctacagccct gagcgagtgc ttatgactgg
      601 tgctgcggtt aaggttttcg atggtgttgt atcggtttaa gagaagcttg gaagcttgag
      661 aaagaggtgc ccgaaagtga gcagagacta cgaaatcgaa ctggaaaaca gggttgcatt
      721 tataaaaagg gttctggcag aaagcggtgc cagtggaatt gtttacggaa acagcggcgg
      781 caaggacagt gcgctggtgg gcattctctg caaaaaagct tgtgaagaca ctttagggct
      841 gatcatgccc tgcgccacga agcggaacta tgtagaagat atggaagatg cgcttacagt
      901 tgccaaggag tacggcatca aaacagaagt ggtagatctg actcctttga aaaagctttt
      961 tgtggagact cttggtgaac ggttctcact acaggaggtt gcgctccaca accttgcccc
     1021 gaggctgcgg atgacgacct tgtatgcagt agctgccagc cagaaccggc tggttgcagg
     1081 tacaggcaat agaagtgaag gttatgtagg gtacttcacc aaatggggag acggagctta
     1141 cgactttaac cctatcgccg acttgaccgt aacagaagtt taccagtttc tggatttcct
     1201 aggtgctccg gagtgcatta tgaaaaaagc cccatctgca ggtttgtttg acgggcagac
     1261 cgatgaaggc gagatgggac tgacctataa agagctggat agcctcctcc ttggcggtac
     1321 ggtacgctcg gaggttttgg aagccattga acgattgcac aaaagaagcg aacacaagag
     1381 gcgcccgcct ttggtatatg gaggttgaca atgaaaccaa acacaaatta cttgaactta
     1441 aaagacagct atctgttttc tacagttgca agaaaggttg cggagtacaa aagcaaaaac
     1501 ccgggagcag atattatccg cttgggcatc ggagacgtca ctctgcctct acctggggtt
     1561 gtagtggagg ccatgggtaa agcagttttg gagatggggg acaagaagac tttccgaggc
     1621 tacggagatg agcagggtta cgtctttttg cgggaagcaa tcttaggcta ctacagtagg
     1681 aaaaacgtgt ctctggaact ggatgaggtg tttatcagcg atggagccaa gagcgactta
     1741 ggtaacattc tggacatctt ctccgtggat aacaccgtgg ttattccaga tcctgtgtat
     1801 ccggtctatg tggataccaa cactatggca gggcgcagca tcgtctttct tgatgcaaac
     1861 ctagacaacg ggttcctgcc tcttccagac gactctctcc aagctgatat tatctacctc
     1921 tgctcgccaa acaaccccac aggtgcggtt tacaccaagg agatgttgca gaagtgggta
     1981 gatttcgctc tggagagaaa tgcggttatt ctgtttgatg ctgcttacga agcttacgtg
     2041 cgcgatccct ctcttcccac cagcatctac cagatagaag gggcaaaagc ctgtgccatt
     2101 gagttctgtt ctctttctaa aactgcaggg tttaccggga ccaggtgtgg atatacagtt
     2161 gtgcccaaag agctggtctt cgatggcgtt tccctgaaca aactttggct gagaaggcag
     2221 actaccaagt ttaacggtgt accctatatt gtacagcgcg gtgcagaagc ggtgttcacc
     2281 gagagaggtc tccaggaagt tggggagtgc atctcctact acttggaaaa cgccaagatc
     2341 atccgcgaaa ctctagagtc aatgggaata gagtttacag gtggagcaaa ctctccttac
     2401 atctggctga agtgtccagg gggaatgtcc tcatgggagt ttttcgacta cctgttggaa
     2461 aaagccaatg tggtaggcac acccggtgtt ggatttggca aaaacggcga gggccatttc
     2521 aggcttacgg cctttggcga cagagaaaac gtacttactg ctgtgggaag gttaaaatcc
     2581 ttgatatagt gcgagctcag ctggctgcta gaagcgatac ttctaggggc cagttttttt
     2641 gtgttcatgt acggtttagg tgccaagatt attacaccaa agaagctttt tcgtgttgtc
     2701 atgatcctgt atggactgaa ttttttggca atcaaagaac acatcgtgaa atgtctggct
     2761 atcacaagga caaaagcccg tagtggagaa atctaacaat aagagtgtac ttgctttctg
     2821 tggaggtgct tgcaatgact gagtacttcg cacacagtgc aggcaaaagt ggaagaaaag
     2881 aatacgtgat agaacatttg acggaagtag ccaaacatgc acaggtgttt gcagctcctt
     2941 ggggagcgga aagcgaagct tacgtagctg ctttagctca cgatttgggg aagtattccc
     3001 agcgctttca acaagttctg gaaaagcagg cgacaagaat tgatcacgca acgcctggag
     3061 cgaaagtcct tttggagaag tacaaaaacg ctggttttgc ctcggcactg gcagtggaag
     3121 gtcatcacga agggattaag cgaggttctc ccaaagaact gttagatcga atccgtatgg
     3181 acaaaggctt ttccgagact caaagcgagt actccgagcg ggacattcaa tccttgatag
     3241 aaaggctatt aagagacggt ggaatccttc cagatcatat tgagagttcg tatcttgacg
     3301 tacataaaga ccctgtcagg gcgcaattat acgtccgaat gttgttttct gcacttaccg
     3361 atgcagattt tctggctaca gaagcgcatt ttaacaggac gaatgatgaa tacgagtacc
     3421 gaaaaatgca aaacgatctc aacgaagcta tgatcgataa ggttctcgat cggcttctaa
     3481 gccatattga gaaaattaag aaggaaaaga agagcagttc ggagatgcag tcgcttagag
     3541 atgatctgct ggagaactgt ttgaaaaagg cttctctccc aacaggcgtg ttcacactta
     3601 ctgcaccaac gggatcggga aaaacgtttt caatgcttgc gtttgctcta actcatgcta
     3661 aagagcatcg gcttaggaga ctaatctttg tgctgcccta cataaacatc atcgaccaga
     3721 tggctaaaca gttcaaagac gttgtaaatc aggtagatga aggcgttttt gtattggaag
     3781 accacagtct ggtagaaatc gctagtgatt ctgacgaact caagtatctc gctgagaatt
     3841 gggatgcgcc aatcattatt actacgtcgg tacggttttt tgagtctttg ttcgcgaacc
     3901 gctcaaatga ctgccggaaa ctacataata tagcgaaatc ggtcattttg tttgacgaag
     3961 cacaaaccat gccctcccat ctggcaccac catcgttgat cgcgttatct gagttagtcg
     4021 acaggtatgg atgcacggtg gttttctcaa cagcgactca accagccttt acttcgttga
     4081 atccggagat cgctaagctt tccgcgaaca cagtttggaa accgacggaa atcgttgatc
     4141 ccgagctaaa actgtttcaa cgatctaaaa aggtcgacgt tgagtggatt g
//
LOCUS       DAOSDA010000110         4174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13427_length_4174_cov_3.478029, whole genome shotgun sequence.
ACCESSION   DAOSDA010000110 DAOSDA010000000
VERSION     DAOSDA010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13427_length_4174_cov_3.478029"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..706)
                     /gene="nth"
                     /locus_tag="PLW82_03540"
     CDS             complement(74..706)
                     /gene="nth"
                     /locus_tag="PLW82_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOJ46379.1"
                     /translation="MTAETRAKKIEKIIGEMYPDASIQLDHSNAFELLVATILSAQCT
                     DKRVNEVTKRIFPKYNTPDKLASLGEANLIPLIKDCGLYKTKSRNIIKTSEILATKYN
                     NEVPSTREDLESLPGVGRKTCNVVLANAFGIPAFPVDTHVFRVAHRLGLSDSKTPEKV
                     EEDLCNLFEPENWIKLHHQMIIHGRTLCKVQKPLCDKCDLKDLCLYKQTD"
     gene            complement(837..1187)
                     /locus_tag="PLW82_03545"
     CDS             complement(837..1187)
                     /locus_tag="PLW82_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46380.1"
                     /translation="MLKEEFRRYGRITYNEMLERLGKGGKHLSHDFVQLIGALTTAAG
                     FLISLYTALTSNISVVAIIVFIVGILLLVLSRWLAKRDLARRRKASRYLSNLDKKKAE
                     EAQKQQQENNGQQE"
     gene            1304..1639
                     /locus_tag="PLW82_03550"
     CDS             1304..1639
                     /locus_tag="PLW82_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ46381.1"
                     /translation="MEKWRKLTEDVLKETLKEEQDNPKIIVSVLGKDRVGIIAEITSV
                     IAKLGGNIIDINQTIFRELFSMNMVIDISNAEVPFVKFKEELEKKGKELDLMIMAQRE
                     DTFIKMHRI"
     gene            1688..3049
                     /locus_tag="PLW82_03555"
     CDS             1688..3049
                     /locus_tag="PLW82_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HOJ46382.1"
                     /translation="MRITSREIFETIKMVEVENFDIRTVTMGISLRDCQRTKMRDMCD
                     AIYSKIMTKAENLVKVAEDLEAEFGIPIINKRISVTPIALLADSVEDEDLTPIAVTLD
                     RAARDTGVNFIGGFSALVEKGFTQGDKKLLTSIPEALKSTERVCSSVNVASTRAGINM
                     DAVHLMGKAIKETASLTADKDGIGCAKLVVFCNIPEDNPFMAGALHGIGEAEAVINIG
                     VSGPGVVRDAISDSPNKDFGSLAEVIKRTAFKITRAGELIGREASKRLGVSFGIVDLS
                     LAPTPEVGDSVAEIIEAMGVERCGGPGTTAALALLNDAVKKGGAMASCYVGGLSGAFI
                     PVSEDAGMIRAVEEGVLTLAKLEAMTSVCSVGLDMIALPGDTAASTISGIIADEMSIG
                     VINKKTTAVRVIPVPGKQVGDFVEFGGLLGKSPILDVSTVSCAGFINRGGRIPAPLQS
                     LIN"
     gene            3075..4082
                     /locus_tag="PLW82_03560"
     CDS             3075..4082
                     /locus_tag="PLW82_03560"
                     /inference="COORDINATES: protein motif:HMM:NF023650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOJ46383.1"
                     /translation="MSANSKTISLTTLILLLIVVAVISYAATWQFVKVRTEDVLPDGI
                     YVGSIKVGGLTIPEALVKVEAKAPMPDRDIELVWSGGSYRLDIDKFKPELDLARIEME
                     LEHVLPHGTFFERLYYKRDLKKSPKKVNIAYRFEEEAFNKLTAELDERIAIPPKDAEF
                     VVNIFDQVSISQEKYGLKVDYNKLLTDLPHKYSYGETVIEVPTVVVAPNYTREDLLAF
                     GIRGIVASFSTQFSLDNPNRVENIRLAAAALDGTILKPGEEFSFNAWVGPRVAELGYK
                     EAQVLLAGGEITEDRWRRVSGFHDSVQHSTSQRTGDCGKTPPLQTCLVRSCWERRGSC
                     L"
     gene            4003..>4174
                     /locus_tag="PLW82_03565"
     CDS             4003..>4174
                     /locus_tag="PLW82_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOJ46384.1"
                     /translation="MEIVERHPHSRPVSYVPAGRDAAVAFDYLDFRFINSQSNYILIT
                     AEVMGDRLLCKVF"
BASE COUNT         1134 a          946 c         1041 g         1053 t
ORIGIN      
        1 ttgattctat cagacacaca ctatttgtcc aaatcaatta gggggcttaa tagttacaac
       61 gctgcacggc gtactaatca gtttgcttgt acaagcacag gtctttgaga tcgcacttgt
      121 cgcacagcgg tttctggact ttgcagagcg ttcttccgtg gatgatcatt tggtgatgca
      181 gcttgatcca gttttccggt tcaaagagat tacaaaggtc ctcttcaact ttttcaggag
      241 tcttgctgtc gctcagcccc aaacggtggg caacgcggaa gacatgggtg tccacgggaa
      301 atgcaggaat gccaaaagca ttagccaaaa ctacgttaca ggtcttcctg ccgactccag
      361 ggaggctttc aaggtcctct ctcgtacttg gcacttcatt gttgtattta gtagctaaga
      421 tctccgaagt cttgatgatg ttccggcttt tggttttgta aagtccgcag tctttgatca
      481 ggggaatcaa gttcgcttct ccaagggaag cgagcttatc cggggtattg tatttgggaa
      541 agatcctttt ggtaacctcg tttaccctct tgtcggtgca ctgggccgac aaaatggtgg
      601 caaccaagag ttcaaaggca ttggagtgat ccagctggat cgaggcatcg gggtacatct
      661 cgccgatgat cttttcgatt tttttcgctc tagtctctgc tgtcaatggc aatcacctca
      721 cgaaagaaca gttcgcatca cactagttct gttggtggtg caaagcgcta acctacttga
      781 taaactaccc gcatttcggt ttttgaaagc aaaaaaagcg actgagaaca gtcgctttat
      841 tcttgttgtc cgttattttc ttgttgctgt ttctgggctt cttcagcctt cttcttgtcg
      901 agattactca agtaccggct ggcttttctt cttctagcca gatctctctt cgccaaccag
      961 cgggagagga ccaggagaag tatacccaca ataaaaacaa taatcgcaac aacgctaata
     1021 ttgcttgtca atgctgtata taacgaaatc aaaaacccag cagctgttgt tagcgctccg
     1081 atcaattgta caaaatcgtg actcaagtgt ttccctcctt tacccaaacg ttccagcatc
     1141 tcattatagg ttattctacc atatctcctg aattcctctt ttaacatttt ctagctggtc
     1201 tcttggcact tccgagttgg tttttcagga gcttagacgc tttgtaagct ttggcattcg
     1261 tgatatactt taataattaa ccaagatctt tggaggagat tgcatggaaa aatggcggaa
     1321 gctgacggaa gatgttttga aagagacact taaagaagag caggataatc cgaagataat
     1381 cgtttctgtt ctaggtaaag acagggtagg tatcatcgcc gagatcacct ctgtcattgc
     1441 taagctcggc ggtaatatta tcgacatcaa ccagactatt ttccgagagc tattttcgat
     1501 gaacatggtt atcgacatct ctaatgcgga agtcccattc gtcaaattca aagaagaact
     1561 ggaaaaaaag ggcaaagaac tcgatctaat gattatggca cagagagaag acacttttat
     1621 taaaatgcac cgtatataga cccgagctaa gcagagaaac agcctatgag aacaggagtg
     1681 gccaaggttg cgtattacat ccagagagat ttttgagacc ataaaaatgg tcgaagtgga
     1741 gaacttcgat attagaaccg taactatggg aatcagtctg agagactgcc agagaaccaa
     1801 aatgcgcgac atgtgcgatg ccatctacag caaaatcatg accaaagccg agaacctggt
     1861 gaaagtcgcc gaagatctcg aagcagagtt tggcattccc atcatcaata agcggatctc
     1921 cgtaactccc atcgctcttc tggccgattc ggtagaagat gaggatctca cccccattgc
     1981 cgtaacttta gacagagcgg cacgagatac cggggtgaac ttcatcggtg gcttttccgc
     2041 attggtggaa aaaggcttta cccaagggga caaaaagctt ctaacctcaa ttcctgaggc
     2101 gttaaagtcc acagagagag tatgttcttc ggtgaatgtg gcaagcacca gggctggaat
     2161 caatatggat gcagtccatc tgatgggcaa ggccatcaag gaaacggcga gcctcaccgc
     2221 cgataaagac ggaatcggct gcgccaaatt ggtggtgttc tgcaatatcc cagaggacaa
     2281 cccttttatg gccggagctc tgcacggcat cggcgaagct gaagcggtca tcaacatcgg
     2341 tgtcagcgga cctggagttg tgagagatgc catcagcgat agcccaaata aagatttcgg
     2401 aagtcttgca gaggtcatca agagaactgc cttcaaaatc accagagcag gagagctgat
     2461 cggcagagaa gcatcaaaaa ggctcggcgt tagtttcggc atcgttgacc tttcgctggc
     2521 gccaacccca gaagtgggag acagcgtggc cgagatcatc gaagctatgg gcgtcgaaag
     2581 atgcggaggt ccgggcacaa ctgcggcact tgcgctcctt aacgatgcgg tgaaaaaagg
     2641 cggagccatg gcatcatgtt acgttggagg tctttcagga gcattcatcc ctgtgagcga
     2701 agatgccggt atgatcagag cggtagagga aggcgttctg acccttgcca aactcgaagc
     2761 catgacgtcg gtatgctcag tgggcttgga tatgatcgct cttcccggcg acaccgccgc
     2821 atcgacaatt tcaggaataa tcgccgatga aatgagtata ggagttatta ataagaagac
     2881 tacagccgtt agagtgatac ccgttcccgg aaagcaagtt ggtgactttg tggagttcgg
     2941 cggactttta ggcaagtcgc cgattctgga tgtttctaca gttagctgtg caggttttat
     3001 taacagagga ggcaggatcc ctgccccgct gcagagcttg attaactaaa gatctgtgtt
     3061 gggagaggtt cgacatgtct gctaactcta aaaccataag tctcacaact ttaatactgc
     3121 tgctcattgt agttgccgtg atttcgtatg cggccacatg gcagtttgtc aaagttagaa
     3181 ctgaagacgt acttcctgac ggaatctatg tgggtagcat aaaagtcggc ggcctgacca
     3241 tcccagaagc tttggttaag gtagaggcaa aagcacctat gccggacaga gatatcgagc
     3301 tggtctggtc tggaggttcc tacaggctgg acatcgacaa gttcaaaccg gagctggatc
     3361 tggccaggat cgaaatggaa ctggagcatg ttcttcccca cggaactttt ttcgaaaggc
     3421 tctactacaa aagagaccta aagaagagtc caaagaaggt gaacatcgcc taccgcttcg
     3481 aggaggaagc gttcaacaag ctcactgcag aactggacga gagaatcgcc atacctccta
     3541 aggacgcgga gtttgttgta aacatctttg accaggtaag catatctcaa gagaaatacg
     3601 gattaaaagt tgactacaac aaacttctca cagatctacc acataagtat tcttacggcg
     3661 aaacagttat agaggtaccg acagtggtag ttgctcccaa ctatacaaga gaagatctgc
     3721 ttgcctttgg catcagaggc attgtcgcgt ccttcagtac acagttcagc ctagacaatc
     3781 ccaatagagt tgagaacatc aggcttgctg ctgcagccct cgatggaaca atcctaaaac
     3841 caggtgaaga gttctcgttt aacgcttggg tgggtccaag agtcgcagaa ctaggctaca
     3901 aggaagccca agtgctcttg gcaggcggag agataaccga agatcggtgg aggcgtgtgt
     3961 caggtttcca cgactctgta caacacagca cttctcagcg gactggagat tgtggaaaga
     4021 cacccccact ccagacctgt ctcgtacgtt cctgctggga gagacgcggc agttgccttt
     4081 gattatctgg acttcaggtt tatcaactcc cagtccaact acattttgat cacagctgaa
     4141 gtgatgggag atcggctttt gtgcaaggtg ttcg
//
LOCUS       DAOSDA010000111         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13449_length_4170_cov_3.531956, whole genome shotgun sequence.
ACCESSION   DAOSDA010000111 DAOSDA010000000
VERSION     DAOSDA010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13449_length_4170_cov_3.531956"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..147)
                     /locus_tag="PLW82_03570"
     tRNA            complement(59..147)
                     /locus_tag="PLW82_03570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Leu,seq:caa)
     gene            complement(214..1203)
                     /locus_tag="PLW82_03575"
     CDS             complement(214..1203)
                     /locus_tag="PLW82_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HOJ46385.1"
                     /translation="MKIGKLSPDLLKSSVLRFEGAKRPEVLVGALLGEDSAIIDMGDQ
                     LLAISVDPITGASAYIGRLAVHVSCNDVVSNGAEPFAIQVLLLMSVDNTVEEVEAIMK
                     EISETAREIGVQVVGGHTEFTDKVTEPIIAIIALGKVTRNKLSTSTGAKPGDRIVISK
                     GAGIEGTAILAEEYRDMLSELSETVLDKAARFYDMISIVPEGRIATSQGVRVMHDATE
                     GGVIGALWEVAEASNVGFTVDRDKVPVRLETKAICDKLGIDPLRLLSSGTLIMITPEP
                     DELLSELQKAGIDAAVIGLIEENPERRVIVCQGEEVPVTGPTTDELWRLKSPK"
     gene            complement(1206..1655)
                     /locus_tag="PLW82_03580"
     CDS             complement(1206..1655)
                     /locus_tag="PLW82_03580"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HOJ46386.1"
                     /translation="MTQGEKRLERALRKAQLEYQKGIRVGPYEVDFIVHGRTIIEVDG
                     YSHVLPEKARIDREKEIYLREKGFHVIRVLSDQTKDAHSMRKLVKTLAKPKPMKHNKP
                     MAYRPFAELKEHLDLGEVQRDKTGREEMLEWLNRYEDRIPASKDGED"
     gene            complement(1709..2641)
                     /locus_tag="PLW82_03585"
     CDS             complement(1709..2641)
                     /locus_tag="PLW82_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46387.1"
                     /translation="MLLVALFLFLTLNTYAQTPFEVWENVKVTENVPVTYDEIVVTTE
                     AQGVSVRYFNVFRKNASTIRREETGYYGDEVLQVELKIDYTDYLWMENIPYVYKSLAS
                     DQLVSWVPTQVSKNTFDGIDVLILTLVAESVPYQARQFISAANMTVFREELYRSGHLV
                     QVRDRRNIRLNPQFEEGTFDVPEGFVVLSDRKAWEKALVSEMLRKSNIQEVYYPEWLP
                     PQWVLIDVNIDEHLDDLFVVYRYSDGSEFYSLFVSPYKGSPKRSRLSVTFQDNMRVYQ
                     LVQDDRIMTLVGRLSERDALKVLESLVELNSTVE"
     gene            complement(2647..3045)
                     /locus_tag="PLW82_03590"
     CDS             complement(2647..3045)
                     /locus_tag="PLW82_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOJ46388.1"
                     /translation="MRCPSHENLSAYVDRELTLSEMQKIETHLQDCLTCRIEVARLEK
                     LIRKFNVLAETDLPIPNMYNPTISRKNYRWAAAAVVVLLVAGSAVFFGLRYNQSSVAE
                     MEEELQYFYNNHKDYVRSQSTAPITLVSWE"
     gene            complement(3051..3626)
                     /locus_tag="PLW82_03595"
     CDS             complement(3051..3626)
                     /locus_tag="PLW82_03595"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ46389.1"
                     /translation="MALEERLKDHSLVQKLKAGDEDAYAQLCTEYSGKMYRLVCRLAG
                     PEDADDLVQEALMQLYKSMGSFKGACSIGTWIYRITTNVCQDYLRKKSRRSWRHLFSL
                     DWLSSETNKEVPSLDFEPSERAEIRDDLDKLRMAIAKLPPEQRAVLVLHDLEQLTYQE
                     VADVLGIVVGTVKSRLFYARRKVRSMLEGGV"
     gene            3852..4037
                     /locus_tag="PLW82_03600"
     CDS             3852..4037
                     /locus_tag="PLW82_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOJ46390.1"
                     /translation="MEVRIDPDLCIACGLCEEICPEIFELGDRDHAVVIGDPEGYEDE
                     VREAAESCPTSAIIVEE"
BASE COUNT          983 a         1080 c          918 g         1189 t
ORIGIN      
        1 ccctcaacct ctgccttggc aaggcagcgt tctagccaat tgaactacat ccgcagtttg
       61 gtgccgaagg ccggaatcga accggcacgt ggtgtaaccc acggcagatt ttgagtctgc
      121 tgcgtctgcc agttccgcca cttcggcaca tagtagctca cgaactcgct gacaatcctt
      181 attatatgcc gaagactatt ataatgtcaa cacctatttc ggacttttta agcgccaaag
      241 ctcatcagta gttggaccgg taaccggtac ttcttcgccc tggcaaacga tcacccttct
      301 ctcagggttc tcttcaatca agccgataac tgcggcatct atacccgcct tctgtagctc
      361 ggacaaaagc tcgtccggtt ctggagtgat catgatcagc gtacctgagg acaggagcct
      421 cagagggtct atacccagct tgtcgcagat agccttagtc tcgaggcgca caggcacttt
      481 gtccctgtca actgtaaagc ccacgtttga agcttcagca acctcccaaa gtgcgccaat
      541 cactccgcct tcggtagcat cgtgcatgac tctcacgcct tgagaagtgg cgatgcgacc
      601 ctcaggaact atacttatca tatcatagaa ccgtgccgct ttgtccagta ccgtctcgga
      661 tagttcagac agcatatccc gatattcttc tgccaaaatc gctgttcctt caatacccgc
      721 acctttactg attacaatcc tatcccctgg ttttgctccg gtgctagtag agagcttgtt
      781 tcttgtaacc ttccccagag cgatgatggc aattatcggt tcggtgactt tatcggtgaa
      841 ctctgtgtgt ccgccgacaa cctgcacccc tatctcacgt gcagtctccg aaatctcctt
      901 catgatggct tctacttctt ctacggtgtt gtccaccgac atgagaagta gaacctggat
      961 ggcaaaaggc tctgccccat tggaaaccac atcattacag gaaacgtgga cggcgagcct
     1021 gccaatgtaa gcgctagcgc cggtgatcgg atccactgaa atagctagaa gctgatcccc
     1081 catatcaatt atggcactat cttcgcccaa gagagctcca accaaaacct caggcctctt
     1141 ggctccctca aaacgcagca cagaactttt taagagatcc ggcgaaagct tacctatctt
     1201 catccctagt cctccccgtc tttcgaggct ggtattctgt cttcatatcg attcaaccac
     1261 tcgagcatct cttctctacc agtcttgtct ctttgtacct cgccaagatc gagatgttcc
     1321 ttcaactcgg cgaatggtct gtatgccata ggcttattat gcttcatcgg tttcggtttc
     1381 gcaagggttt ttaccagctt ccgcatggaa tgcgcgtctt tggtttgatc gctgagaacc
     1441 cggatgacgt ggaaaccttt ttctcggaga taaatctctt tttccctgtc tatgcgtgct
     1501 ttctccggta gcacgtgact gtagccgtct acttctatga ttgtccttcc atgtacaata
     1561 aagtcgacct cataggggcc gactctgatg cctttttgat actctaattg tgctttgcga
     1621 agagccctct cgagcctctt ttcgccttgg gtcattttca cggaccagcc cctcaactgt
     1681 tgcctctctg tgtcagagaa agtcttttct attcaacggt ggagttaagt tctactaaag
     1741 actctagcac cttcagcgca tctctttcac tcagccgtcc cacaagggtc atgatccggt
     1801 cgtcctgtac cagctgatac acgcgcatgt tgtcctggaa ggttacggaa agccggcttc
     1861 gcttcgggga acccttgtat gggctgacaa acagagagta gaactccgat ccgtcgctgt
     1921 agcggtagac tacaaataga tcatctaggt gctcgtcgat attgacatct attaaaaccc
     1981 attgtggtgg gagccattcg ggatagtaga cctcttggat gttgcttttg cggagcattt
     2041 cgctaacaag tgccttctcc caagctttgc gatcagaaag caccacgaag ccttcaggta
     2101 catcaaaagt cccttcttca aactgaggat taagcctaat gttcctccga tctctgacct
     2161 ggactagatg cccgcttctg tagagctctt cgcggaacac tgtcatattt gcggcgctaa
     2221 tgaactgtct agcttgataa ggcactgatt ctgcaacaag ggtcaggatc aacacgtcaa
     2281 taccgtcaaa ggtgttcttt gacacttggg taggcaccca acttaccagt tggtcgcttg
     2341 caagactctt gtagacgtaa ggaatgtttt ccatccatag gtaatctgtg tagtcgattt
     2401 tgagctcaac ttggagcact tcatcaccgt agtagccggt ctcctctctc cttatcgtgc
     2461 tggcattctt tctgaagacg ttgaaatagc ggaccgaaac accttgtgct tctgttgtta
     2521 ccactatttc atcataggta actgggacgt tttctgtgac ttttacgttt tcccaaactt
     2581 caaaaggagt ttgggcgtag gtgtttaacg ttagaaatag aaatagtgcg accagaagca
     2641 tcgacttcac tcccaagaaa ccaaagtgat cggcgcagtg ctttgacttc tgacgtaatc
     2701 tttgtggtta ttgtagaagt attggagttc ttcttccatc tcagcaacac tactctggtt
     2761 gtagcgaagg ccgaagaaaa cggcacttcc cgcaacaagt agcactacta cagcagccgc
     2821 tgcccacctg tagttctttc tagatattgt tggattgtac atgtttggga tggggaggtc
     2881 tgtctctgca agaacgttga acttgcgaat gagcttctcg agacgtgcca cttcaattct
     2941 gcaggttagg caatcctgaa ggtgagtttc gatcttctgc atttcagata gggttaattc
     3001 tctatctaca tatgctgaca ggttttcatg actaggacat ctcatttcac tcaaacacct
     3061 ccctcgagca tggatctgac ctttctcctg gcgtaaaaca gccgcgactt tacagttcca
     3121 actactatcc ccaacacatc tgcgacttct tggtaggtca gctgctctaa atcgtggagt
     3181 actaaaaccg ctctctgttc cggcggtagt ttggctattg ccattctcag tttatccaag
     3241 tcatctctaa tctctgcccg ttcgcttggt tcgaagtcta gcgaaggaac ctctttgttt
     3301 gtttcgctgc taagccagtc caaggaaaaa agatgcctcc agctgcgtct gcttttcttt
     3361 ctcaggtagt cctgacacac attggttgtg atccggtaga tccaggttcc gatgctgcat
     3421 gcccctttga aagaacccat ggacttatag agctgcatta atgcttcctg gaccaaatcg
     3481 tcggcatctt caggaccagc taagcgacaa accagacgat acatctttcc cgagtactcc
     3541 gtgcagagct gggcatatgc gtcttcgtcg ccagctttga gcttctgcac cagagaatga
     3601 tcttttaacc tttcctctaa agccatgttc cttaccacct ttacttagca gcctaaaaac
     3661 attactatat ttatagacgc actacagttc taattgttca aacccatact taaagaagct
     3721 attcgcaaaa agtgtctgaa tcacctgctg ggctgtgtat agccgcgtac aaagcggggt
     3781 atcctgcgtt tagaaagcgc cgcgcttttt aacggataga tgtcaaattc atatctcaag
     3841 gaggctttac tatggaagta aggatcgatc ctgatttgtg tatcgcttgt ggcctatgtg
     3901 aagagatctg ccctgaaatt tttgagttag gagaccgcga tcacgctgtc gtcatcgggg
     3961 atcctgaagg ttatgaagac gaggttagag aagcagccga aagctgccca acatctgcta
     4021 tcattgtcga agagtaactt gtttcggagt taagaggata gaaacttagg tttttcttga
     4081 agtacatctc cagattagtc ttttacgata attaaaaggg gctgttgcac aattagccga
     4141 ttaagctgat cgtgcggcag cctttttttg
//
LOCUS       DAOSDA010000112         4141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13570_length_4141_cov_3.686490, whole genome shotgun sequence.
ACCESSION   DAOSDA010000112 DAOSDA010000000
VERSION     DAOSDA010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13570_length_4141_cov_3.686490"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..101
                     /locus_tag="PLW82_03605"
     CDS             6..101
                     /locus_tag="PLW82_03605"
                     /inference="COORDINATES: protein motif:HMM:NF016215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HOJ46391.1"
                     /translation="MGSGFSARIKPDSFGASTVADRFTKGGSRKA"
     gene            357..617
                     /locus_tag="PLW82_03610"
     CDS             357..617
                     /locus_tag="PLW82_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HOJ46392.1"
                     /translation="MSGPSVIDKIRADLAELVGKKVKIRANKGRRQVMEAEGVIESIY
                     PNIFVIKLSEDHALSRVTYSYTDVLTETVELRVDNKRIGLGA"
     gene            complement(769..1467)
                     /locus_tag="PLW82_03615"
     CDS             complement(769..1467)
                     /locus_tag="PLW82_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOJ46393.1"
                     /translation="MEKQSETLSLSYQSQILTLILSENECHKCIRIAKERNVQSGIVI
                     VGRGTVKSSVLNFLGIKSQKKEVVNFLLPNEEAEELLESFAQELELHKPGHGIAFATP
                     VLKSSRGVNVKEDTQSTELSLEVNGMFKKVTVIVDRGMAEEIMDAARKAGVTGGTIMH
                     GRGAGAEHTSTLFGVEIEPEKELVVMLIPSSLVDKVAKALTEEFHLNEPGKGILFVEP
                     IIKAIGLYQPKESK"
     gene            complement(1400..2983)
                     /locus_tag="PLW82_03620"
     CDS             complement(1400..2983)
                     /locus_tag="PLW82_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOJ46394.1"
                     /translation="MNVLTAKLKEVLASVLPITFIVLALNFTISPLESDMIFAFLIGS
                     VLVIVGLTIFLFGIDQGLEPIGQGIGSTMTHSRSFAALVTISLILGFFVSFAEPDLRI
                     LANQVHAVTSGQFNSLLMVIVVSIGIGVMMTLGMLRILGGVRLKYVFSATYGLIFLFS
                     LFSTSDFLAIAFDASGATTGAITVPFMLALAAGIASKKKDSTMNEADSFGLVGISSAG
                     AILGVLITGLILGVDKLDGALPEPVIRSGSLLQVYSAQLMHIAWDAFLSLLPIIVMYI
                     LFQVYSFKHKKGKVIDVTRGIILTFVGLVIFLLGVNSGFMYLGTELGVQLAGMSSKFP
                     VLLVSLLLGLTTVLAEPAVHVLTQQVETVTGGSVKRILVFVFLSVAVGMSILMSTVRI
                     LVPGIKLWMYLLPGFGLCAILAFFVPDLFVGMAYDAGGVASGPMTATFSLAFVQGISA
                     SIPGADIVAEGFGMISVVAMMPVLAIELLGALYQAKLRKAAKTEEAVKTSLSGGSHGK
                     TIGDSEPLVSESNTDANSE"
     gene            3284..>4141
                     /locus_tag="PLW82_03625"
     CDS             3284..>4141
                     /locus_tag="PLW82_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HOJ46395.1"
                     /translation="MSALKLGKRGKKVEKLQEKLRSLGYDPGEIDGWFGYKTLESVSE
                     LRHDLHLPPTGEVNRQVYACLKTRKRFGSSYERSEDGLLERTFMPKIGSFGQLNGADF
                     ELSLLDNDRTKIKVLKLEGAAFLTTQLVKIRKHPFYIAFHTFKGQKKAQVLALGRKAE
                     GFVYLPWKARPVLNEPIIGRDTEENLQSLIKELYWKDLYLGIPLVAFEWLIEPEDAGG
                     NVRYSDLSVDYLDSGVVREGYPKEVPYDEAIGYLRKRGKRGRKIQGDLLFQYRRKHAT
                     TIIKFISPDE"
BASE COUNT         1165 a          998 c         1006 g          972 t
ORIGIN      
        1 tgattgtggg cagcggcttt tccgcaagga tcaagcccga ttcgtttgga gcaagcactg
       61 ttgccgacag gttcactaaa ggcggaagcc ggaaagcttg aaaaaactgc attctctgag
      121 atgttctggc aaaaataggt ccaaatgccc aaaatagcgc cagttacccc agactttatt
      181 tataaattgg ctgttgacag gcgcaaagca tattggtata attttgtctt gacgcttagt
      241 tagaaatgtg ttaaaattaa ctaggcgtgg gctatttata gatattttca gagaggtttt
      301 ttgcagtacc gatgtctgtc tgcccaccta tattttttat gagggggcgt cgttgaatga
      361 gtggacctag cgtaatagat aaaattagag cagatctagc tgagcttgtc gggaaaaagg
      421 taaagattag agccaacaag ggcagacgcc aagtcatgga agcagaaggt gtaatcgaaa
      481 gcatttatcc caatatattt gtcatcaaac tatcggaaga tcatgccctg tcgcgagtga
      541 catacagcta tacagatgta ctaacagaaa ccgttgaact gcgtgtagat aacaagcgca
      601 tcggtcttgg agcataggaa aggggactaa gttcagtcct cttttttttg taagtagcta
      661 cccctctact aaaaccatac ataactgaaa cagtaaaagg ctgtcccgtg cgggacagcc
      721 tttccttaat gtgctcttct atgagtaagc gcagcaattc aagctaacct acttactttc
      781 cttcggctga tagaggccga tggctttgat aatgggctct acaaacagaa tacctttacc
      841 aggttcattg aggtggaact cctcagtcaa cgctttggca accttgtcta ccaggctact
      901 tgggataagc ataacgacta gctctttttc cggctcaatc tccacgccga acagggttga
      961 tgtgtgttct gcgcctgcgc ctctgccgtg catgatagtg ccgcctgtaa caccggcttt
     1021 ccgcgctgcg tccattatct cttccgccat accgcgatca acgatgacgg ttactttctt
     1081 aaacattcca tttacctcca gactcagttc tgtgctttga gtgtcctcct taacgttgac
     1141 acctctgctg gacttaagaa caggtgtagc aaaagcaatg ccgtgtcccg gcttgtggag
     1201 ttccagctcc tgtgcgaagc tctccagcag ttcctctgct tcctcgttgg gcagaaggaa
     1261 gtttaccact tcctttttct ggctctttat ccccaagaag ttcaaaacgg aactcttgac
     1321 cgtgcctcta cccacaatga caataccgct ttgcacattg cgttctttgg caatgcggat
     1381 gcatttgtgg cattcattct cactcagaat tagcgtcagt atttgactct gatacgagag
     1441 gctcagagtc tccgattgtt tttccatggc tacctccaga taaacttgtt ttcacagctt
     1501 cctccgtttt ggctgctttg cggagtttcg cttgatatag agctcccaaa agttcaattg
     1561 ccaaaactgg catcattgca actaccgaaa tcatcccgaa tccttcagcc acaatgtctg
     1621 ccccggggat gcttgcggaa atgccctgaa caaaagccag ggaaaatgtg gcggtcatgg
     1681 gacctgaggc aactccgccg gcatcatagg ccattccgac aaaaaggtcg ggaacaaaga
     1741 acgccaaaat ggcgcacagg ccaaatccgg gaagcaggta catccacagt ttgataccgg
     1801 gaacaaggat tcgtaccgta gacatcaaga tggacattcc tacggctaca gacaaaaaca
     1861 cgaaaaccag aatgcgcttg accgatccac cggtgaccgt ttccacctgc tgcgtgagca
     1921 cgtggacagc aggttcagca agaacggtgg taaggccaag gaggagcgag acgaggagca
     1981 cagggaactt cgaactcatt cccgcaagct gcactcccag ttcggtcccc agatacataa
     2041 agccgctgtt tacacccaaa aggaagatca caagtcccac gaatgtgagg attattcccc
     2101 tggtgacgtc gataacttta cccttcttat gcttgaaaga atacacctga aagaggatat
     2161 acatcacgat tatggggaga agagacagga aagcgtccca ggcaatgtgc ataagttgag
     2221 cactgtacac ctggagtaga cttcccgagc gaataacagg ttcggggagg gccccgtcta
     2281 gcttatcaac tcccagaatg aggccggtga tcaaaacgcc aaggatagct ccggcagaag
     2341 agatgccaac cagaccgaag ctgtccgctt cattcatggt actgtctttt ttcttcgacg
     2401 caatacctgc cgctagagcc agcataaagg gtaccgtaat ggctcctgtg gtggcaccgg
     2461 atgcgtcaaa tgcgatagcc aaaaagtccg atgtggaaaa cagggaaaag agaaaaatca
     2521 gtccgtaagt tgcggagaaa acatatttca atctcacgcc tccgaggatc cgcagcatgc
     2581 cgagggtcat catcacgcca ataccgatgg agacaacaat taccatcagg agactgttaa
     2641 actggccgga cgtgaccgca tgcacctgat tggccaaaat gcgcagatcc ggttctgcaa
     2701 aagagacaaa gaaaccgaga atgagactta tggtcactag agctgcaaag ctgcgggaat
     2761 gggtcatggt ggatccaatg ccctgaccga taggctcaag accctggtcg ataccgaaca
     2821 agaagatcgt aagtccaaca attaccagca ccgacccgat gagaaacgca aagatcatat
     2881 cggactccag cggacttatg gtaaaattca gtgccaacac gataaaggtt atcggcagca
     2941 cagatgcgag tacttctttc agtttcgcag ttagtacgtt caagcagtac accccaatct
     3001 tcgccgagtt ggcaagaagt ctgatctttt aagatagtct cattaaacag catcgatttt
     3061 gcgcgttcac tactaataat ccctgtttaa acaccacatg agcttatggc gcagcaatcg
     3121 tgtagatgca aatgatccgt ttttctagag aaggcccgat gggttatcgg acttcccgga
     3181 ataaacggtc ttttgcgttt tttaaagctt tacccaaaac aagctaaact cctccatgtc
     3241 taaacgaaaa aaacggcgca taggatgtgg cggggggata gtggtgagtg ccctgaaact
     3301 tggaaaacgc ggaaagaaag tagaaaagct acaggaaaag ctgaggtctc tcggctacga
     3361 tcccggggag atcgacggct ggtttggcta taaaactctg gagtcggttt cggagttaag
     3421 gcatgacttg cacctgcctc ccacaggaga agtgaacagg caggtgtatg cgtgtctaaa
     3481 gactcgcaaa cgcttcggct caagctacga aagaagcgaa gacggccttt tagagcgtac
     3541 cttcatgccg aagatcggat ctttcgggca attaaacggt gcagattttg agctgtctct
     3601 tttagataac gaccgcacga agatcaaagt gctgaagcta gaaggtgctg cgtttctaac
     3661 tacgcagctg gtaaagatca gaaaacaccc cttctacatt gcctttcaca ccttcaaagg
     3721 acagaaaaag gctcaggttc ttgctctcgg aaggaaggct gaaggctttg tctatctacc
     3781 atggaaggca aggccggtgc tcaacgagcc cataatcggc agagacaccg aagagaacct
     3841 gcagtcgctc attaaagaac tgtactggaa ggacctctac ctgggcatcc cccttgtggc
     3901 ttttgagtgg cttattgaac cggaagatgc cggcggaaat gtccgctact cagatctaag
     3961 cgtcgattat ttggacagcg gggtagtgag ggaaggctat cccaaggaag tcccctacga
     4021 tgaagctatt ggctatctca gaaaaagagg aaagagaggc cgcaaaatcc agggagacct
     4081 cctcttccag taccggagga aacacgcaac aacgatcatc aagttcatat cacctgacga
     4141 g
//
LOCUS       DAOSDA010000113         4132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13607_length_4132_cov_3.288693, whole genome shotgun sequence.
ACCESSION   DAOSDA010000113 DAOSDA010000000
VERSION     DAOSDA010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13607_length_4132_cov_3.288693"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="PLW82_03630"
     CDS             complement(<1..1328)
                     /locus_tag="PLW82_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOJ46396.1"
                     /translation="MKSVIMAGGMGTRLKPLTCLKPKPLVPFMGKPLIDHVMDHLEAN
                     GIKEHILTLFHLPQMIIDHVSVQDRVVDFIIEKEPKGTAGSVKEAAEFLKNTFVVASG
                     DSITDIDLKKALEFHRKKGALATIVLTRVPEPLEYGVVLTNADGKITRFLEKPTWGEV
                     FSDTVNTGIYILEPEVLEYIPKGRSYDFSRDLFPALLKAGLPLLGYIAEGYWSDVGSI
                     IQYRESHFDVLRGMVKSVKPVIDEAADIDSTARIVQPVSIFKGAKIGANAKIGPFTVV
                     GKGAVVGKGCEVAYSIVGGKAYLGDGVTAEGTIVDERAYVGEGSKLSESTIVAEDTKI
                     GERTILTPLVKVYPQKYVGPMSVLNTDLIYGSLEGGHLFTEEGLRGKLHRDLTVDILL
                     KTGVAFSEVQGARVLGAASSGDDKAEFVQRILSLGANGKGADVVDFGEQVV"
     gene            complement(1285..2550)
                     /locus_tag="PLW82_03635"
     CDS             complement(1285..2550)
                     /locus_tag="PLW82_03635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ46397.1"
                     /translation="MRVLMVSWEYPPKIVGGLGRHVEGLSEALVRAGHEVTVVTGDSP
                     KLPEKQVVQGVEVLRALPHKPPGRDFVEEVMLLNFNMVERMVSELVGLSTAFDVVHVH
                     DWLGAYAGKLFKHALRLPLIATIHATEFGRNGGLHNDLQRHISDLEWWLCYEAWQVIV
                     CSRYMEGEVQHVFGLPRDKLKVIPNGIDPAKLLIRGHADPSEVRARFAHPDEKIVLFV
                     GRLVREKGVWVMLDAFKDLLGVVPEAKLVVVGKGPEELYLRDYARRLGLEHKVYFTGF
                     VDDETRNHLYHLCSVAVFPSLYEPFGIVALEAMALGKPVVVSDTGGLSEVVSHGLTGM
                     KFPPGDSRALAGVLADILLDFNLAQNLAREGKREADLYTWDRVAKSTSDVYREVLRSA
                     QASGWSVRRELGGRSFEVSHYGRWNGNKA"
     gene            complement(2590..3456)
                     /locus_tag="PLW82_03640"
     CDS             complement(2590..3456)
                     /locus_tag="PLW82_03640"
                     /inference="COORDINATES: protein motif:HMM:NF021500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HOJ46398.1"
                     /translation="MIWFLILLLVLAPPAIGGEMIFEMADRSGDDFGEGSLVYPTHIS
                     FAPYKDLFDLTHFTATVDGEYLLLDLVFGEITNPWKAPEGFFHQHILVFIGTETDTGT
                     KHYEPLGLVFNEPYTYVVRIAPWSLSTLRNYEGQVLAQITVGTIDAQTIRAYIPLEHI
                     TKPTRSWRYAVLVGGYDAFGRDLFREVLARNTDWEFGGGGSFPFVDILAPSFGKEKQQ
                     DQLRQGLFYLQDSRQGPRTYGLLAVAITLLLAGFVAGAFVLYRRYGFPFRFRLKLRRR
                     EHNKKGSVSRWN"
     gene            complement(3584..>4132)
                     /locus_tag="PLW82_03645"
     CDS             complement(3584..>4132)
                     /locus_tag="PLW82_03645"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOJ46399.1"
                     /translation="PANLGSSVGITKAKDRGQLRTALEVAFQYDTRVVVEEAVKNPRE
                     IECSVLGNERLFVSIPGEIVPDDEFYTYDAKYHSEGSKLLIPAELSEDQVQTVQELAI
                     KAFRAVGASGLARVDFLVEGDTGKVLLNEINTMPGFTAISMYPKLWEKTPEGYTYGKL
                     VSELAALALERYKKRKALKITR"
BASE COUNT          955 a         1154 c          943 g         1080 t
ORIGIN      
        1 accacttgtt ctccgaaatc tacgacatct gcgcctttac cgtttgcacc taagctcaaa
       61 attctctgca caaactccgc tttgtcatcg ccactgcttg cagcacccaa gactcgtgct
      121 ccctgcacct ccgaaaaagc aactcctgtc ttgaggagta tgtccactgt gagatcgcgg
      181 tgcagctttc ctctcagccc ttcttcggtg aagagatgtc ctccctcaag tgacccgtag
      241 atcaagtctg tgttgagaac agacatagga ccaacgtact tctgggggta gaccttcacg
      301 aggggagtca ggattgttct ctcgccgatc tttgtgtctt ctgcaacaat ggtgctttcc
      361 gaaagcttgg aaccttcacc gacataagcc ctctcgtcca cgatggtccc ctctgcggta
      421 accccatctc cgagataggc cttccctccc acaatagagt aagcaacttc gcaccctttc
      481 cccaccactg caccctttcc cactacggtg aatgggccga ttttagcgtt ggcaccgatt
      541 ttcgcaccct taaagatgct cacaggctgc acaatccgtg ctgttgaatc gatatcagct
      601 gcttcatcga ttaccggttt gacgcttttc accatgcccc gcaggacatc gaaatgggac
      661 tctctatatt ggatgatgct ccctacatcc gaccaatacc cttcggcgat gtaacctaac
      721 aggggaagtc cggctttaag cagagcaggg aagagatctc tgctgaagtc atagcttctg
      781 cctttgggaa tgtactccaa tacctccggc tcgagaatat agattcccgt attaaccgtg
      841 tcgctgaaaa cctcacccca ggttggcttt tcaaggaacc ttgtgatctt tccatcggca
      901 ttggttagga ctacgccgta ctccaaaggt tcaggaactc ttgtcagaac aatggtggca
      961 agtgcgcctt ttttgcggtg aaactccaag gccttcttca agtcgatatc ggttatggaa
     1021 tcgcctgaag ctaccacgaa agtgtttttt aggaactcag ctgcttcttt gacgctgcct
     1081 gcagtaccct taggttcttt ttcaatgatg aagtccacaa ctctatcctg cacgctcaca
     1141 tggtcaatga tcatctgggg aagatggaag agagttaaaa tgtgttcttt gatcccgttt
     1201 gcttctaaat ggtccataac gtgatcgatc aagggtttcc ccataaacgg aacaaggggc
     1261 tttggtttca agcaagtcaa cggtttaagc cttgttccca ttccacctgc cataatgact
     1321 gacttcaaaa gatctacctc ccaactcgcg gcgcacgctc caaccgcttg cctgcgccga
     1381 tcttagcact tctctgtaca catcgcttgt gctttttgct accctgtccc aggtgtagag
     1441 gtccgcttct cgctttcctt ctcttgcaag gttttgtgcc aggttaaagt ccaaaagaat
     1501 gtctgccaga accccagcca atgcccgaga gtctcctgga gggaatttca tccctgtaag
     1561 tccgtggctg acaacctcgc ttagcccacc tgtatcactc accacaactg gcttgcctaa
     1621 tgccatagct tcaagtgcaa ctatgccaaa cggctcgtat aggctaggga aaaccgctac
     1681 gctgcagaga tggtacaagt ggtttcgggt ttcatcatcc acgaagccgg taaagtagac
     1741 cttgtgctct aggcccaacc ttcttgcgta gtccctgagg tatagttctt caggcccttt
     1801 ccctaccacc accagcttgg cctctggcac tacgccgaga agatccttaa acgcatcgag
     1861 catgacccag acgccctttt ccctcaccag tcttcccaca aacaagacga tcttttcatc
     1921 tggatgagcg aaacgtgccc tcacctctga agggtctgca tgtccgcgta tcaggagttt
     1981 tgctgggtct attccattgg gaatgacttt gagcttgtcc ctgggcagac caaaaacgtg
     2041 ttggacttct ccttccatat agcggctgca cacgattacc tgccacgctt cgtagcagag
     2101 ccaccattcg agatcggaga tgtggcgctg caggtcgtta tgcaagccgc cgtttctgcc
     2161 aaactcagtg gcatggattg tcgctatgag aggaagtcgc agtgcgtgtt taaacagctt
     2221 tccggcatag gcccccagcc aatcgtggac gtgaaccaca tcgaatgcag tgcttaaacc
     2281 tactagctcg ctcaccatcc tctccaccat attgaagttc agcagcatga cctcttccac
     2341 aaaatcgcga cctggaggtt tatgggggag ggctcggaga acctcgacac cttgaaccac
     2401 ttgtttttca ggaagcttgg ggctgtctcc ggtcacaacc gttacctcgt gtccagcccg
     2461 aactaacgct tcagacagtc cctcaacgtg ccgtcctagc cctccaacga tcttaggggg
     2521 atactcccaa ctcaccatga gtacacgcat agttccacct gctttgactt attctggcag
     2581 gatcagcctc tagttccacc tggaaacact gcctttttta ttatgctctc ttctcctcag
     2641 ctttagccta aatctgaagg gaaatccgta tctccggtac aggacaaatg ctcctgcaac
     2701 aaaacctgcc agaagaagag taatggccac ggcaagtagc ccgtaggtgc gtgggccttg
     2761 cctgctgtct tgtaggtaaa acagtccctg cctcagctgg tcctgctgtt tttccttgcc
     2821 aaagctcggt gcgagaatgt ccacaaacgg aaagctgccc cctccaccaa actcccaatc
     2881 ggtgttccta gctaggacct cgcgaaacag gtctcttccg aaggcatcgt atcccccgac
     2941 aagcactgca tatctccagc tcctcgtagg ttttgtgatg tgctccagag gaatgtatgc
     3001 tctgatggtt tgggcatcga tggtacctac ggtgatttgt gccagaacct ggccttcgta
     3061 gtttctgaga gtgctgagag accacggagc tatcctaacc acatacgtgt agggttcgtt
     3121 aaacaccaag cccaagggct cgtaatgctt agttccggta tccgtttctg taccgatgaa
     3181 gaccagtatg tgctggtgga agaagccttc cggagctttc cagggattgg ttatctcgcc
     3241 aaagacaagg tctaaaagca ggtactcacc atcaactgta gctgtgaagt gagtaagatc
     3301 gaacaaatcc ttgtaaggtg caaaagagat gtgggttggg tatactaggc ttccttctcc
     3361 aaagtcgtca ccgcttcggt ctgccatttc aaagatcatc tcaccaccaa tggccggtgg
     3421 ggctaaaaca aggagcagaa ttaagaacca aatcatcggg ttcacctcct gaaaaaaaga
     3481 aaacacagtt cgctttctca agctcactgt gtctgtatgc agtagttttc agttttagac
     3541 caagaaactg aggaggcagg gctttaaccg cttgcttcgc tcgttacctt gtaatcttga
     3601 gtgccttgcg ctttttgtac ctttccagag caagagctgc cagttccgac accaacttcc
     3661 cgtaggtgta gccttcgggt gttttttccc agagtttggg gtacatgctg atagcggtaa
     3721 agcctggcat ggtgttgatt tcattcaaga gaaccttccc cgtatctcct tcaacaagaa
     3781 agtctacccg tgcaagacct gatgcgccta cggctctgaa agctttgatg gcaagttctt
     3841 ggactgtttg cacctggtcc tcggaaagtt ccgctggtat aagcagcttc gaaccttccg
     3901 aatggtactt ggcatcgtag gtgtagaact cgtcatcagg tacgatctcc cctggtatgg
     3961 aaacgaaaag gcgttcgttt cctagcacgc tgcactcgat ctctcgagga ttcttcacag
     4021 cttcttccac aaccactctg gtgtcgtact gaaaagctac ttccagggcg gttctaagct
     4081 gaccgcgatc tttagctttc gtgatgccca cagatgaccc caagtttgca gg
//
LOCUS       DAOSDA010000114         4119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13661_length_4119_cov_3.376969, whole genome shotgun sequence.
ACCESSION   DAOSDA010000114 DAOSDA010000000
VERSION     DAOSDA010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13661_length_4119_cov_3.376969"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PLW82_03650"
     CDS             complement(<1..917)
                     /locus_tag="PLW82_03650"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ46400.1"
                     /translation="MGFFIEQQHESLTKTGETLCGDYVAVRRTPESFIAVLSDGLGSG
                     VKANILATLTARIASTMLAQGSTLQEVVQTMIATLPKCKVRGIAYSTFTAVQVFRDGR
                     LYIAESDNPELLFFREGKLNQLPRRELVIEGKTIYETSFSLQENDVLVFFSDGVENAG
                     IGGIRPLGWPKEEIARECEEIIATRGERFEARDITEHIRDRCREFYRSKIGDDTTVMC
                     LRVRRPRQLAIAIGPPQEKTRDREFVEDFLKKPGKKAVCGGTTSTIIARELGKELKVN
                     LDSLTGEPPMGEIEGIDLVTEGIITLCKA"
     gene            complement(928..2601)
                     /locus_tag="PLW82_03655"
     CDS             complement(928..2601)
                     /locus_tag="PLW82_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOJ46401.1"
                     /translation="MGLIKTVEANCRDCYKCVRYCPVKAINVLDGRAQIDEERCVLCS
                     RCIPVCPQNARSVFNQIDYVKKLLKGPKPVVLSLPPTFPGAIEESEEEFASLLHKTGF
                     SRVFESAEAAGPILDYYRQADRPLISSVCPGVVSLVEKYFPELKNRLAPVLSPVIAHS
                     KYLRGLFPGEHLVYAVSCEAQKAEILKEPEVDAVLTLSELMTLLREVGGDLKAYTDRK
                     DGEIALKAEFRERTMGIEGAFSKAVGYDVPFDTRSAVVSGFENCMDFLKKMQQQRIGR
                     EVEFIEIWLCQGGCINGAGVPDTLTLNERKERVLQFARSASEGDKQSVQLEDISREFV
                     EKPVEVTVPTPKQLEEILASMGKRTKADELDCGACGYDTCREKAKAVFRGAAEIEMCI
                     PYMKTRAESMNDVIIQATPNGIIVCDTDLRIVTINPAAEKMFQCKQETLKGKKVSALV
                     DDGIFREVLETKRLIARNVEYPAYGLKVRHFVFYVPKSQVVIAIFADITEQSRQQDQL
                     SELRKETLERAQEVINKQMRVAQEIASLLGETTAETKVTLDQLIRIIKG"
     gene            complement(2606..2857)
                     /locus_tag="PLW82_03660"
     CDS             complement(2606..2857)
                     /locus_tag="PLW82_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOJ46402.1"
                     /translation="MISIQVCVGSSCHLKGAHDVVQAFKREISSRKLDQEVFLTGAFC
                     LGRCIDGVSIKIDDELILGVTPESVPEIFLNRVVSVLGG"
     gene            complement(3009..3446)
                     /locus_tag="PLW82_03665"
     CDS             complement(3009..3446)
                     /locus_tag="PLW82_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014488137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOJ46403.1"
                     /translation="MKKIALVSACLLGVPTRYDGTGKQNAEVLALSKEYLLIPFCPEQ
                     LGGLSTPRKPAEIVGERVINVDGEDVTEFFVRGAQVSLQIAEKIGPNVVILKEGSPSC
                     GVHFVYDGTFQGRKVEGQGLTAKLLREKYPVLCENDLKKLEQV"
     gene            complement(3443..4063)
                     /locus_tag="PLW82_03670"
     CDS             complement(3443..4063)
                     /locus_tag="PLW82_03670"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOJ46404.1"
                     /translation="MHILLLGGSPETLLWDRGPEGSWDVVVCADGGYNLALKWGIEPD
                     WIIGDGDSTFVPYPEHIKQEKHPARKDATDCELAIDKAVELGASQVTLVGFLGGRLDH
                     TLANILGIARRHEVRCVFEEKWGRVFFAEKSNDIFGKKGDLLSLIPVGGKCEGVTTWG
                     LEYPLKSETLHPFSTRGISNVFLGDSCSVTVETGELLVVHLRSGVE"
BASE COUNT          946 a         1173 c          923 g         1077 t
ORIGIN      
        1 atggctttac agagagtaat gatgccttct gtaaccagat cgatcccctc aatctcgccc
       61 atgggcggct cccctgtaag gctatctaag ttgaccttaa gttcttttcc cagctccctg
      121 gcaatgatgg tggaagttgt tccgccgcag acggcctttt tacccggctt tttcaagaag
      181 tcttcgacaa actctctgtc gcgagtcttc tcttgaggcg ggccgatggc tatcgcaagc
      241 tgtctgggtc tgcgaactct gaggcacatc acggtggtat cgtctccgat cttactgcgg
      301 taaaactctc ggcatctatc cctgatgtgc tcggtgatat ccctggcctc aaagcgctct
      361 cctctggtgg cgatgatctc ttcgcactct cgtgcgattt cttccttcgg ccagcccaaa
      421 ggacgaatgc cgccaatccc ggcgttttcc actccatcgc tgaaaaagac caaaacgtcg
      481 ttttcctgga ggctgaagct ggtttcgtaa atcgttttac cttcaataac cagctcccgt
      541 cttggcagct ggttaagctt tccttctcta aaaaacagaa gttcgggatt gtcgctttcg
      601 gctatgtaca gccgcccatc tctaaacacc tgaactgctg taaaagtcga gtatgcaatc
      661 cctctaactt tgcacttcgg cagagttgca atcatggtct gcacaacttc ctgcagcgta
      721 gagccttgtg ctagcattgt ggaggcaatc cttgctgtga gagttgccag aatattggct
      781 tttactccgg agccaagacc gtcggacaaa acggcaataa acgactcagg cgttctgcgg
      841 acggcaacat agtcgccgca gagggtctcg ccggtttttg tcagactctc gtgctgctgt
      901 tctataaaaa aacccacagc ttgtcaccta acctttaatg atgcgaatca gctggtctag
      961 tgtcactttg gtttctgccg tcgtttcgcc gaggaggctg gcaatttcct gggctacccg
     1021 catctgttta ttgataacct cctgagctct ctcgagagtt tcctttctca gttcggaaag
     1081 ctgatcttgc tgccgcgatt gctcggtgat atcggcaaaa atagcgataa ccacctggga
     1141 cttcggcacg taaaacacaa aatgccttac tttgaggcca taagctgggt actccacgtt
     1201 ccttgcaata agccgctttg tctccagcac ctccctgaag atgccgtcat ccactaaagc
     1261 agaaaccttc ttccctttca gcgtctcctg cttgcattga aacatcttct ctgctgcggg
     1321 attgatggta acaatccgca aatctgtatc gcaaacgatg ataccgttgg gtgtggcttg
     1381 aatgatcaca tcgttcatgg actctgcacg ggtcttcatg tagggaatgc acatttcaat
     1441 ctctgcggct ccccggaaga cggctttcgc cttttcgcgg caggtgtcgt agccgcaggc
     1501 accgcagtcg agctcatcgg ctttggtacg cttgcccatt gaggcgagaa tctcctcaag
     1561 ctgtttgggc gtgggtactg taacctccac tggtttttcc acaaactccc gggagatgtc
     1621 ctcaagctga acgctctgct tatctccttc ggaagcactg cgggcaaact gcaaaactct
     1681 ctcttttctt tcgtttaaag ttaaagtgtc aggcacgcct gctccattga tgcagcctcc
     1741 ctgacatagc cagatctcta taaactccac ttctcggccg attctctgct gctgcatttt
     1801 tttcaggaaa tccatgcaat tttcaaagcc acttacaact gcacttctcg tatcgaaagg
     1861 cacgtcgtac ccgaccgcct tggaaaaagc accttcaatc cccatggtgc gctcgcgaaa
     1921 ctccgctttt aaggcaatct caccgtcttt gcggtcggtg taagctttga gatcgcctcc
     1981 aacctctcgg agaagggtca tgagctcgga cagagtcaaa accgcatcca cctcgggctc
     2041 tttgagaatc tctgcttttt gagcctcaca agagactgca taaaccaggt gttcccctgg
     2101 aaacagaccc ctgaggtact tgctgtgggc aatcacagga gatagtaccg gagccagacg
     2161 attcttcagc tcggggaagt acttctcaac tagacttacc acaccaggac agacgctgga
     2221 gatcagcggt ctatctgctt gacggtagta gtccagaatg ggtcctgcgg cttctgccga
     2281 ctcaaaaact ctactgaacc cggttttgtg gaggagcgac gcaaactctt cttcgctttc
     2341 ctctatcgca ccggggaacg taggcggcag ggacaacaca actggcttgg gtcccttcag
     2401 aagttttttt acataatcta tctgattgaa gacgctgcgg gcattttgcg ggcatacagg
     2461 tatgcacctg ctgcagagta cgcacctttc ctcatcgatc tgagcccgcc catctaaaac
     2521 gttgatagct ttaacgggac aatagcgcac gcatttgtag cagtcgcggc agttagcctc
     2581 gacggtttta atcagtccca tcagcttaac ctcctaaaac gctgaccact ctgtttagga
     2641 aaatctccgg cacgctttct ggcgtgacgc ccaaaatcag ctcgtcgtcg atcttgatag
     2701 acacgccgtc aatgcaccgg cccaagcaaa acgcacctgt taaaaacact tcttgatcca
     2761 acttgcgaga gctgatctct cttttaaatg cctgtaccac atcatgggca cctttcaggt
     2821 ggcacgagct gccaacacaa acttgaatgg agatcaactt ttaaacctcc gatcatctat
     2881 cttgaccaac agtcagagcc taaaaaccgc ttaatttcaa cgtttaaagg tctgtttgta
     2941 acaaatttaa caatctctat tttgaattat agcacagggc ttaggcaatg gcaaccatag
     3001 tcagccctct aaacctgctc aagctttttc aggtcgtttt cgcacaaaac cgggtacttt
     3061 tctctcaaga gttttgcggt gagaccctga ccttcaacct ttctcccttg aaacgttccg
     3121 tcatacacaa aatggacgcc acatgacggg ctgccttctt tgaggatcac cacattggga
     3181 ccgatcttct cggcgatctg cagagacacc tgtgcgcccc tgacaaaaaa ctccgtgaca
     3241 tcctcaccgt ctacgttgat caccctttcg cctacgatct ctgcaggttt tctcggcgtt
     3301 gaaagtccgc cgagctgctc aggacagaag gggatgagca ggtactcctt tgatagagcc
     3361 agcacttctg cgttctgttt tcctgtgccg tcgtaccttg taggaacgcc caaaaggcaa
     3421 gcggacacaa gggcgatttt tttcactcta caccactcct taaatgcact accagaagct
     3481 cgccagtttc cacagtgacg ctgcaactgt cgcccaagaa cacattggag attcccctag
     3541 tgctaaaggg gtgcagcgtt tcgcttttta gcggatactc caatccccat gtagtaactc
     3601 cttcgcactt tccacctaca ggtattaggg aaagcaagtc gccttttttg ccgaaaatgt
     3661 cattggactt ttctgcaaaa aacaccctcc cccacttctc ctcaaaaacg caccgtacct
     3721 catgtctgcg ggcgatgccc aggatattgg cgagtgtatg gtcgagtctt cctcctagga
     3781 aaccaacaag agtaacttgc gacgctccga gctccacagc cttgtctata gcgagctcgc
     3841 agtctgtggc atctttcctg gcgggatgtt tttcctgctt aatatgttcg ggatacggca
     3901 caaaagtcga gtctccatcg ccaatgatcc aatcaggttc gattccccac ttcagcgcca
     3961 ggttataacc gccatcggca cagacgacca cgtcccatga gccttcggga ccgcggtccc
     4021 aaagcagcgt ctcggggctt cccccgagga gcagaatatg cacaagatca ccttctttgc
     4081 ttgtcctgcc aacttacaga agctctccgg ctacacaga
//
LOCUS       DAOSDA010000115         4064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13939_length_4064_cov_2.927413, whole genome shotgun sequence.
ACCESSION   DAOSDA010000115 DAOSDA010000000
VERSION     DAOSDA010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13939_length_4064_cov_2.927413"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLW82_03675"
     CDS             complement(<1..183)
                     /locus_tag="PLW82_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46405.1"
                     /translation="MELQRLILKNKNKIALISGVLIAAAFLNHWLMHVPVISELFLIS
                     ASVIGVLPIAIQAYQAL"
     gene            complement(248..541)
                     /locus_tag="PLW82_03680"
     CDS             complement(248..541)
                     /locus_tag="PLW82_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein, ric"
                     /protein_id="HOJ46406.1"
                     /translation="MTRFNEVKEKHLSKLVMYVPVVERVHGGHHPEFYEVRKVFNEIQ
                     AKLEAKETETPDLSQEFARLREITGGYTVPGDVCETYEAVYNMLAELDRAYHE"
     gene            925..2442
                     /locus_tag="PLW82_03685"
     CDS             925..2442
                     /locus_tag="PLW82_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ46407.1"
                     /translation="MTSTTLEMGNRASGSTEKLLFALFDVLPVPLAAFASDGATVFVN
                     EAFLIDFNVFSPAKVVGKLNLLKHLVPENERIEESFFRIMGGEKLSISGVRILFPEFA
                     TVYRGGFLAKHELVGFPIYPYDEDVPKVVTVFISKNVHQIRQDIEQAMEFLEEHWLDD
                     FNLDKLCTCCGLSRNHLRRTFKDLLGITLYRYHQTIKIEKMKALLASSENSISDIFSC
                     CGADYYSTLAKAFKKATGVSPKDYKDSLKFEALARKNHLKDLRKTSHFLEKVLMETCN
                     NLPIPVGVSKPNGDIAFVNEAFSTMLSSKGFPLISHGHDPLREPLKDLSPEIEEGFVR
                     ARNGETVLLSHAKLPLDVFGPRIQKKGTGDGLIALYAHILVFPIFKEKQLKYVGWVLF
                     TSRVYEGKHEVEKAKEFLENNWKEDFYIEQAAAAAGVSPTHLVRLFKEDTGETPYHYY
                     LQLKITHLKEVLKDRNLSIAQAFLACGFKNPKNSARFFKEQVGMTPSTYRRLLGQ"
     gene            complement(2559..3464)
                     /locus_tag="PLW82_03690"
     CDS             complement(2559..3464)
                     /locus_tag="PLW82_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ46408.1"
                     /translation="MDFSEKLRTLRKQHGLSQEELAHRLGVSRQAVSKWENGQGFPDT
                     DKLLVLSNMFAVSLDYLLKNGDVGETVHTDSRYFVSPDLAGEYFAFKKKRGYQIAIGV
                     ALLVFSLSFTLFFSGPLGVVLFLLVAALGLAVLVMLAFQPDRMRFFELERRPLAFDPG
                     FLQRFQDDFAAEQRRCGLKIALAVFLIILSFTLAFIIDEGGYGDQYLGVMPMVWALAV
                     GLIIAAGTTIGAGHVILNNPDHVEEVEEEERQSWIYAATMPLSGVLYLALGFIWNAWH
                     PGWLVFPLTAILSYAYSAWQSARRS"
     gene            complement(3636..4013)
                     /locus_tag="PLW82_03695"
     CDS             complement(3636..4013)
                     /locus_tag="PLW82_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOJ46409.1"
                     /translation="MQYDAQTPKEYLEMLEDDWRKERLLEVRQLILDLAPELTETVRY
                     KMLTYEAGEQFIFGLNAQKGYVSLYIGDIDKIEGARALLEPFDLGKGCIRIRKTVDIS
                     KTGLEDFIKAVVEMHRKGKDTSC"
BASE COUNT         1088 a         1002 c          873 g         1101 t
ORIGIN      
        1 aagagcttga taagcttgaa tagcaatagg aaggactccg atgaccgatg cagagattaa
       61 aaacagctcg gaaatcaccg gtacgtgcat cagccagtga ttgagaaatg ccgcggctat
      121 gagaacacca gagatcaacg ctatcttatt cttgtttttc agtatgagtc gctgcaattc
      181 catcactacc cttcatctga acctggtgga aagcacttgc attaaaccta agctgtcgta
      241 agagctttta ctcatggtaa gccctgtcca actctgcaag catgttgtat actgcctcgt
      301 aagtttcgca gacgtctcca ggtacggtgt agcctccggt gatttctctt agccgagcaa
      361 actcttggct tagatctgga gtttctgttt cttttgcctc aagctttgcc tgaatctcgt
      421 tgaatacttt acggacctca tagaactcgg gatggtgtcc accgtgtact ctctccacta
      481 ctggtacgta catcaccagt ttactgaggt gtttctcttt cacttcgttg aatctggtca
      541 tgggttgctt ctctccttca cagtttgctt catttcacgt gtattgtaac atcagacgta
      601 atgaaaaaat atgattcaaa tcaaatttgg ctctgaactt atcttttcag cacaaatgtc
      661 cctcagaggc atatgtcaag atgaactact cattctccag ccaatggcat ttaaggattg
      721 ttttgtgcgg ttggcatatg gccacatgga tcaagcttgc tctttttttc tcaaaccaca
      781 ggcctaatac aaggagtctc tttttgattt atggaagttt acacataact cattctatgt
      841 tttgccgatc acgtaaaaac gccttttgtg agtttgctct gcttcacaat tccttgcctt
      901 accgggcgta gaaggagcgt tatcatgacc agtaccacac tagaaatggg caatagagcc
      961 tctggatcca cggaaaaact gctattcgcc cttttcgacg ttctcccggt tcccttagca
     1021 gcattcgcaa gcgacggtgc aacggttttt gttaatgaag cgtttcttat agatttcaac
     1081 gtttttagtc ctgcgaaagt ggttggtaag ctaaacctgc tcaaacatct tgtcccagag
     1141 aacgagagga ttgaagagtc cttctttcgg attatgggtg gagaaaagct ttccatctct
     1201 ggtgttcgca tactctttcc cgagtttgcc accgtatata gaggcggttt tttagcaaaa
     1261 cacgaactgg taggttttcc catatacccc tacgatgaag atgttccgaa agttgtgaca
     1321 gtgttcatct ccaagaatgt tcatcagatc cgtcaggaca tcgagcaagc catggagttt
     1381 ttggaagaac actggcttga tgattttaac ttggacaagc tgtgtacctg ctgcggcctg
     1441 tcccggaatc acctaaggcg cacctttaag gatctactgg gaataactct gtaccgctat
     1501 caccagacca ttaaaatcga aaaaatgaaa gcgcttcttg caagttcaga aaacagtata
     1561 agcgatatct tttcctgctg cggagcggac tactacagca ctcttgccaa agctttcaaa
     1621 aaggcgaccg gagtgagccc caaagactat aaggacagtc tgaagttcga agctctcgca
     1681 agaaaaaacc acttgaaaga cctgcgcaag acaagccact ttctggaaaa ggtactgatg
     1741 gaaacctgca acaacctgcc tattcctgtt ggagtctcca aaccaaatgg agacattgcg
     1801 ttcgtgaacg aagcctttag cacaatgctg agcagcaaag gttttcccct catatcacac
     1861 ggacacgatc cgctgcgtga acctctaaag gacctttcgc ctgagatcga agaagggttc
     1921 gttagggcaa gaaatggtga aaccgttctc ctatcccacg caaaacttcc tttggatgtc
     1981 tttggaccta ggattcagaa aaagggaaca ggcgatgggt tgattgctct gtacgctcat
     2041 atcctggttt tccccatttt caaagaaaaa cagctgaagt acgtaggctg ggttctcttt
     2101 accagcagag tatatgaagg caagcatgag gtcgaaaagg ccaaggagtt cctggaaaac
     2161 aactggaaag aagacttcta cattgaacag gcggctgctg cagcaggggt tagccctact
     2221 catcttgtaa ggcttttcaa agaagacacc ggcgaaaccc catatcatta ctatcttcaa
     2281 ctcaagatca cccatctcaa agaagtcctt aaagatcgca acttgagcat cgcccaagct
     2341 tttctagctt gcggtttcaa gaacccgaag aactcagcga ggttcttcaa agaacaagtg
     2401 ggcatgacgc catctaccta ccgcaggttg cttggccaat gataagtgca aaacggagag
     2461 accaccgccg aacttggctt tggtctctcc gttttacacc tattcttaag acttagcggc
     2521 aatcagctct tgtactttac ttccgcagaa ggtggatttt aagatctccg tgcgctctgc
     2581 catgcgctgt aagcgtagct taaaattgcg gtcagaggaa ataccaacca tccgggatgc
     2641 cacgcgttcc aaatgaagcc cagtgcaaga taaagaactc ccgaaagtgg catggtagca
     2701 gcgtagatcc agctttgtct ctcctcctct tctacttctt ccacatggtc gggattgttt
     2761 aaaatgacat gtccggctcc aatggtggtt cccgcagcga tgatcaaccc tacagccaaa
     2821 gcccagacca taggcatcac tcccaggtac tggtcaccat accctccctc atcgataatg
     2881 aatgctagag taaagctcag aatgatgaga aagaccgcca aagcgatttt caatccgcat
     2941 cttctctgct cagctgcaaa gtcgtcctga aatctctgta agaaacccgg atcgaaagcc
     3001 agtggacgtc tctccagttc aaagaatctc attctgtccg gctgaaacgc cagcatcact
     3061 aaaaccgcaa gacccagcgc tgccacaagg agaaacaaca ccactccaag aggaccggag
     3121 aaaaacaaag taaatgacag tgaaaaaacg agcagagcta cacctatggc gatctggtat
     3181 cctctctttt tcttaaaagc gaagtactct ccagctaagt cagggctaac gaagtatctg
     3241 gagtcggtgt gtacagtctc ccctacatct ccgtttttga gaagatagtc tagggacaca
     3301 gcgaacatat tgcttaaaac cagcagcttg tctgtgtccg gaaagccctg tccgttctcc
     3361 catttactta ctgcctgtct ggaaacgcca agtcgatggg caagttcttc ttgagaaaga
     3421 ccgtgctgtt tcctcagcgt tcttagtttt tctgaaaaat ccatgttgtt cacctcattt
     3481 cggaaccaat tctatcattt ggcaaagtag cgtactacca attcccctta gctttttgtc
     3541 aacttcaagt tgcgttgttg aagcttcaag ctttggacga cttggttcac gaatcgaaga
     3601 atagggtata cctctaaagg aggctttgaa gacagtcagc aggaagtgtc ctttcccttg
     3661 cggtgcatct caactaccgc tttaatgaaa tcctcaagac cggtcttgga gatatctacg
     3721 gtctttctaa tccttatgca gcccttgcca agatcgaagg gctctagtag ggctcttgca
     3781 ccttcgatct tatcgatgtc gccaatatac aaactcacgt accctttctg agcgtttagg
     3841 ccgaagataa actgctctcc agcctcataa gtcaacattt tgtacctgac ggtttctgtt
     3901 agctccggtg caaggtccaa aattaactgc cttacctcca gaagccgctc ttttcgccaa
     3961 tcgtcttcaa gcatttccag gtattccttg ggggtttggg catcgtattg catagtcatt
     4021 ctcctcatgc ttagtttgcc gtgtcaaagg cttaaactgc atat
//
LOCUS       DAOSDA010000116         4057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13974_length_4057_cov_3.589205, whole genome shotgun sequence.
ACCESSION   DAOSDA010000116 DAOSDA010000000
VERSION     DAOSDA010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13974_length_4057_cov_3.589205"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /gene="wecB"
                     /locus_tag="PLW82_03700"
     CDS             complement(<1..798)
                     /gene="wecB"
                     /locus_tag="PLW82_03700"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOJ46410.1"
                     /translation="MARKIKTLIVLGTRPEAIKVAKVIKTLKADPNFAVTVVSTGQHR
                     EMLDQVLELFAITPDVDLGIMKPSQTLTGITRNVLGGLEEVFRKIQPDIVLVHGDTST
                     AFSSALSAFYHGLKVGHIEAGLRSFTRREPFPEEINRRLISQLADLHFAPTPVNKANL
                     ILEGTSAESIYVTGNTVIDSLLEMVEEGYNFQNPVLQSLDFSKRTLLAEIHRRENLEK
                     LEEIYSGLLEIVERFADVQLIYSVHPNPRVKEAAVKVLGGKPRIHLID"
     gene            complement(704..1807)
                     /locus_tag="PLW82_03705"
     CDS             complement(704..1807)
                     /locus_tag="PLW82_03705"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOJ46411.1"
                     /translation="MWQGLKAFAVAFLTTLIATPLVIRLALKMGWVDKPLPRRINKYP
                     VPTLGGLAIFVGFLAASVLSWPVANFRHLPSLYLSGALFFLLGAIDDIYDLSAKLKFW
                     VMLGISVLYVLTGPRIQHLTNPSGGTIDLGILAIPLSVMWLVGTANLVNLIDGLDGLA
                     TGTVAITALTCIFLSSQKLDWFILVLNCGLLGACLGFLPYNFNPAKVFMGDSGALFLG
                     FVVGALAIEGSLKGAATVTLAVPLLALGLPILDTIFSIFRRAKTKKPIYQADGDHLHH
                     RMLHLGLGQKKVVLILYLVNVLFAIGAFLVFNVSPEIGFIYTLIVVCVITIKAARQGV
                     LKVGKKDQDSDSVRDKTGSNQSSQSYQDAESGS"
     gene            complement(1820..2260)
                     /locus_tag="PLW82_03710"
     CDS             complement(1820..2260)
                     /locus_tag="PLW82_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOJ46412.1"
                     /translation="MRPSWDDYFMDIAELVASRSTCLRRQVGAVIVKDRRIISTGYNG
                     APRGVSHCLDVGCLREQLGVPSGERHELCRAVHAEQNAIIQAALHGVSTNGATLYCTT
                     QPCVLCAKMLLNAGIKEIFFRGSYPDELALSLLQEGDVKINLLK"
     gene            complement(2269..2898)
                     /gene="upp"
                     /locus_tag="PLW82_03715"
     CDS             complement(2269..2898)
                     /gene="upp"
                     /locus_tag="PLW82_03715"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOJ46413.1"
                     /translation="MGELFVFDHPLIQHKLSIIRDVNTGCKEFRELVNEVSMLMAYEV
                     TKDMALEACEVQTPVAKAKCYHLAGPQLAVVPILRAGLGMVEGILDLIPTARVGHIGV
                     YRDPETLQPVEYYCKLPNDIEERLIIVTDPMLATGGSAIAAINFIKARGGKNIRLMGL
                     IAAPEGIEAIQKAHPDVNIYVAGLDSHLNDHGYIVPGLGDAGDRLFGTK"
     gene            complement(2922..3368)
                     /gene="rpiB"
                     /locus_tag="PLW82_03720"
     CDS             complement(2922..3368)
                     /gene="rpiB"
                     /locus_tag="PLW82_03720"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOJ46414.1"
                     /translation="MQDKIALGSDHGGFELKEIIKKFLEGKGIPYEDYGCYSLESVDY
                     PDIAFTVAEAVAAGKHPFGILVCGTGIGMSIAANKVKGIRAALAGDCYSARMAREHNN
                     SNILVLGGRVVGQELALEIVNAYLNAEFTAGRHQGRINKICQREGC"
     gene            complement(3353..3775)
                     /locus_tag="PLW82_03725"
     CDS             complement(3353..3775)
                     /locus_tag="PLW82_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOJ46415.1"
                     /translation="MAEALLLSELAKDPNLDCIKVKSAGTFASEGSGASPGSINALKA
                     RNIDLTGHKARQLTQALLDESDLVLTMTEHHAEFIRKNFKSGKVHTLKGFVGESGDVL
                     DPFGQGDRVYEETAQEIERLIKKLVKQLKEDDDRCKTK"
     gene            complement(3847..>4057)
                     /locus_tag="PLW82_03730"
     CDS             complement(3847..>4057)
                     /locus_tag="PLW82_03730"
                     /inference="COORDINATES: protein motif:HMM:NF015446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HOJ46416.1"
                     /translation="YKISLGSRSNPLEIATNLYSALRYFDSLDVEFILSETISGEGLE
                     VAINDRLNRASGGKTLEDFLKDIRG"
BASE COUNT         1009 a         1050 c          944 g         1054 t
ORIGIN      
        1 atcgatcagg tgaatgcgag gcttacctcc gaggactttt acagcggctt cttttaccct
       61 gggatttgga tgcaccgagt aaatcagctg cacgtctgca aaccgctcca caatctccag
      121 gagacctgag tagatctctt ccaatttttc caggttctct cttctgtgaa tctccgctaa
      181 aagagtcctt ttggaaaaat ccaagctttg cagaaccggg ttttgaaagt tgtaaccttc
      241 ctctaccatc tctaagagag agtcaatgac cgtgtttccc gtgacgtaga tcgattcggc
      301 gctggttccc tcaagaatca gattggcttt gtttaccggt gtcggtgcga aatggagatc
      361 tgctaactga gagatgagtc tacggtttat ctcttcggga aacggctccc gtctggtaaa
      421 gctgcggagg cctgcttcga tatgccccac tttcaggccg tggtagaaag cactgagagc
      481 tgaagaaaaa gccgttgaag tatctccgtg gaccagcaca atatcgggct ggatcttccg
      541 aaaaacttcc tccaaacctc ctaagacgtt tcgcgtgata cccgtcagag tttgactggg
      601 cttcatgatg ccaagatcca catcgggcgt gatggcaaaa agctcgagaa cctgatcaag
      661 catctcccgg tgctgccctg tggatacaac tgttacagcg aaattaggat ccgctttcag
      721 cgtcttgata actttggcta ctttgattgc ttccggtctt gtccctaaca ctatcagagt
      781 cttgatcttt cttgccaact ttcagcaccc cttgtctggc tgctttaatg gttatgacgc
      841 aaaccacaat gagcgtatag ataaaaccaa tttccggact gacgttgaag accagaaacg
      901 caccgatagc aaacagcacg ttcacaagat acaaaatgag cacgaccttt ttctgcccga
      961 gacctaggtg gagcatacgg tggtgaagat gatctccatc agcttggtag atgggctttt
     1021 tcgtctttgc cctgcggaaa atggaaaaaa tcgtgtccaa aatcggcagc cccaaagcaa
     1081 ggaggggaac tgctaacgtg acagtagcag caccctttaa ggatccttcg atggcaagtg
     1141 ctcctacaac aaacccgagg aagagcgctc ccgagtcgcc catgaaaact ttagccggat
     1201 tgaagttgta ggggagaaat cccaaacacg caccgaggag cccgcagttc aaaaccaaga
     1261 tgaaccaatc aagcttctga ctgctgagaa agatgcaggt tagagctgta atggctaccg
     1321 ttcctgtagc aagcccatcc aacccgtcaa tgaggttcac caagttagcc gttcctacga
     1381 gccacatcac agacagcgga atcgctaaaa tccccaggtc gatagtgccc ccagagggat
     1441 tggtgaggtg ttggattctt gggcccgtta ggacgtacag aacggaaatc ccaagcatta
     1501 cccagaactt caacttggca ctgaggtcgt aaatatcatc tatcgcgccc agcaaaaaaa
     1561 acagcgcacc gctcaagtag agagacggca aatggcgaaa attggcaacc ggccagctca
     1621 acacacttgc tgccagaaag ccgacgaaaa tcgccagtcc ccctagagtt ggaacggggt
     1681 atttattgat gcggcgtggc aaaggtttgt ccacccaacc cattttgagt gcaagccgta
     1741 tgaccaaggg tgttgcaatc agcgtggtta aaaacgctac agcgaaagct tttagtcctt
     1801 gccacattag atgtcacctc tattttagta ggttgatctt gacatctccc tcctggagaa
     1861 gagaaagtgc cagttcatct gggtaagaac ctctgaaaaa aatctccttg atccctgcat
     1921 tgagcagcat ctttgcacag agcacgcagg gctgtgtggt gcagtacagc gtagctccat
     1981 tggtggaaac cccatggagt gctgcttgaa taatggcatt ttgctctgca tgaactgctc
     2041 tgcaaagctc gtggcgctca ccagagggca cacccagctg ctcccggagg catcccacgt
     2101 caaggcagtg ggaaactccc cggggagcac cgttgtatcc ggtgctgatg atacgcctat
     2161 ccttaacaat gaccgctcct acctgcctgc gcaggcatgt ggagcggctg gccacaagct
     2221 cagcaatatc cataaagtag tcatcccatg agggtctcat ggtgtcacct actttgttcc
     2281 gaagagtctg tctccggcat cacccagtcc aggcacgatg tatccatgat cattgagatg
     2341 ggagtccaga cctgcgacgt aaatgtttac atcgggatga gctttttgaa tggcctcaat
     2401 gccttcaggt gctgcgatca aacccatcag tctgatgttc ttgccacctc tggccttgat
     2461 gaagttaatg gcagcaatag ccgatccgcc tgtagcaagc atggggtcgg ttacaatgat
     2521 cagtctttct tcgatgtcgt ttggtagttt gcagtagtat tcaactggct gaagggtctc
     2581 cggatcgcga tagacgccaa tatggcccac tctggctgtg ggaattagat ctaaaatccc
     2641 ttccaccatg ccaagacctg cgcgaagaat aggcactact gccagctgcg gacctgcaag
     2701 atgataacat ttggccttgg ctacaggggt ctgcacttcg caggcctcta gtgccatatc
     2761 tttggttact tcatacgcca ttagcattga gacttcatta actaactctc taaactcttt
     2821 gcatcctgtg ttaacatccc tgattatcga cagtttgtgt tggatcaatg gatggtcaaa
     2881 aacaaatagt tctcccataa tgttctcccc ttctacttag actaacagcc ttctctctgg
     2941 caaatcttat tgattcttcc ttggtgtctg cctgcggtaa actcggcatt taaatatgca
     3001 ttgacgatct caagagcaag ctcttgacct accactctgc cgccgagaac caaaatgtta
     3061 ctgttgttgt gttctctagc cattctggca gagtagcagt cccctgcaag tgcagcacga
     3121 atgcccttga ctttgttggc tgcaatgctc atgccaatgc ctgtcccaca gaccaagata
     3181 ccaaaaggat gcttgcctgc tgccacagct tctgccactg taaaggcaat atcgggataa
     3241 tccactgact cgaggctgta gcagccgtag tcttcatagg ggattccctt gccctcaagg
     3301 aattttttta tgatttcctt gagttcgaag ccgccgtggt cggatcctaa agctattttg
     3361 tcttgcatct atcatcgtcc tctttcagtt gttttacgag ctttttgatc agcctttcga
     3421 tctcttgagc agtctcttca taaaccctat ccccttgtcc aaaaggatca aggacatcgc
     3481 cactttcgcc cacaaatcct ttcagggtgt ggactttgcc ggacttgaag ttcttcctga
     3541 taaactcggc atggtgctct gtcatcgtta ggacaaggtc gctttcgtca aggagagctt
     3601 gagtgagctg cctcgctttg tgacctgtaa ggtcaatgtt tctggccttt agagcattaa
     3661 tgcttcctgg agacgctccg ctgccttcac ttgcaaaagt accagcactc ttgaccttga
     3721 tacagtcaag attcgggtct ttggccagtt ccgacaagag aagcgcttct gccatgggag
     3781 acctgcaagt attacctgtg catacgaaca gcacctgcaa aaagtaccac tccttgcttt
     3841 ctctctttag cctctaatgt ccttcaaaaa atcttctagg gttttacccc cgctagccct
     3901 gttcagcctg tcatttattg ccacttctaa accctctccg gagatggttt cggaaagaat
     3961 aaactccaca tctaagctat cgaaataccg gagtgcagag taaaggtttg tagcaatttc
     4021 caggggattg ctgcggcttc ccaaggagat tttgtag
//
LOCUS       DAOSDA010000117         4054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_13982_length_4054_cov_3.171043, whole genome shotgun sequence.
ACCESSION   DAOSDA010000117 DAOSDA010000000
VERSION     DAOSDA010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13982_length_4054_cov_3.171043"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PLW82_03735"
     CDS             complement(<1..917)
                     /locus_tag="PLW82_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOJ46417.1"
                     /translation="MSVKQAKGPALEQRNLTADLDTNLKIIRELLAESNDVVIREFLI
                     AQKTKAAILCVDGLLDKEVVQRNVLRPLIVDAAETSFVPRYRLKDYVCKNLLTAEDIK
                     EEKDIREILNKILSGDTALLIDKEDAALIIASRGFATRSVTEPSSEAVVRGPRESFTE
                     SMRTNTSLIRRKINNPDLVFESFTLGSVTNTLVTIVYLKGIAKEDLVREVKQRIRQIE
                     IDGIFDTGYIEQLIEEHPYSPFATTGYTEKPDVLAARILEGRVGILVDGSPNAITVPL
                     LFIEKFQTADDYYTRPLFTSLIRIIRILS"
     gene            1198..2856
                     /locus_tag="PLW82_03740"
     CDS             1198..2856
                     /locus_tag="PLW82_03740"
                     /inference="COORDINATES: protein motif:HMM:NF027473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4855 domain-containing protein"
                     /protein_id="HOJ46418.1"
                     /translation="MLLVLLLLLLSCTAFAEGAQEATMTGFKGFRTRSSQMKITDNAL
                     VVSNVAFAEANTGSLLWEDFDLSFKVTIGQLGSPYAGFTLIFRSWGGRDCYALTVAPD
                     RTAVERFHGVYSDSRVLGYVARGMRDGETADYRITAQGNRFTVYKNGEKIIEAVDPDN
                     MYPYGYMAVRAELASLTFADWTLKVSEIRDPVLRKRAEAGYGLPVEPLGGEMLKVQEG
                     DPQNIMLVYDQYTHKNWMTVDALPYVAYLSAKTYMPQDWFFDSFLFLALSAPGGRNFG
                     GGNPAILEDYYWWLDRMFGKEKFGLGAFETAIANAKNVLGDPDHKAKVIMMIPHPSEI
                     EGTPFGEINGERPVLRGNPTEAAKGRLKVAKWWIDEVVKRFNEKGYQNLELVGFYWLA
                     EDLDLGGIDKLYLPDVGDYVRSLGMKFYWIPWYMSPGYDSWKELGFDYSIMQPNYMFD
                     KDIAKTRLEKTAVLAAEANCGIEIEADDSVWTPSGKDRYLDYLRAANTHGWAGDVIKA
                     FYQEVDLFGKAAMSSYPPDREIYDITYGFVKGTWTEKLKFEEEN"
     gene            3040..3558
                     /locus_tag="PLW82_03745"
     CDS             3040..3558
                     /locus_tag="PLW82_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOJ46419.1"
                     /translation="MAVKHVFLALLAKQPMHGYELKNAFEEIAAGSWPLNFGQVYQTL
                     NRLERDGFIESFEVVQEDKPDKKVYQITEDGRDHLKEWLQQDDHWNLFFDEQALRLMA
                     LPLVDSALSGEILKGYRNFILRVIAGLTKMLEEEKDTTSTYYALLERNILRAEADLTW
                     INRMIERWERHA"
     gene            3551..>4054
                     /locus_tag="PLW82_03750"
     CDS             3551..>4054
                     /locus_tag="PLW82_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46420.1"
                     /translation="MLELNGISKVYGQGASQVEALRDVDLNISPGEFVAVMGTSGCGK
                     TTLLNIIGCLDTATSGIYTLQGKDVSRLSLKDKARVRNKTFGFVFQAFNLLAEYTVLE
                     NVTLPLRYSGVPKRRWKEQGLGVLERLGIADLSSRYPDQLSGGQQQRVAIARALVNDP
                     EIIIADEP"
BASE COUNT         1019 a          939 c         1101 g          995 t
ORIGIN      
        1 aacgaaagga tgcggatgat ccggatcagc gaagtgaaaa gaggtctagt gtagtagtcg
       61 tccgcagttt ggaacttctc gataaacagg agggggacgg tgatggcatt gggactccca
      121 tccaccagaa tgcctactct gccttcaagg attctagcgg caaggacatc gggtttttct
      181 gtgtagccgg tagtggcaaa aggactgtaa gggtgctcct cgatcagctg ttcgatgtaa
      241 ccggtgtcga agatgccgtc gatttcgatc tggcgaatcc tctgcttcac ctcgcgaacg
      301 agatcttctt tagcaatccc cttaaggtag acaatggtca caagagtatt ggtcacgctt
      361 ccaagggtaa acgattcgaa aaccagatct gggttgttaa tcttccgtct gatgagcgag
      421 gtgttggtgc gcatggactc ggtaaaggac tccctagggc cgcgcacaac cgcttcgctt
      481 gagggctcgg ttacactccg tgtagcgaaa cctctagaag ctatgatcag tgctgcatct
      541 tccttgtcaa tgagaagggc cgtgtcccct gaaaggattt tgtttaagat ctcccggata
      601 tctttttcct ctttgatgtc ctctgcggtc aggaggtttt tgcagacgta gtctttgagt
      661 ctgtagcggg ggacgaagct cgtctcagct gcatcgacaa tcaaaggccg cagcacgttc
      721 cgctggacca cttctttgtc caggaggccg tcgacacaga ggatggcagc tttagttttt
      781 tgagcgatga gaaactcccg gataaccacg tcgttgcttt cggcaagcag ctctctgatg
      841 atcttcaggt tggtatctaa gtctgcagtg aggtttctct gctctagagc cgggccttta
      901 gcttgtttta cactcatgct gtctaccact cgctttatgc tctttactta agttaaatat
      961 ttgcctcctc gggtttttct atacattcaa acctagctct gcctgatgcc aaagcctaca
     1021 ggatcggggg agtgaaacct tgccaccttc cggctccaat cgacgaaaac accgcttcgc
     1081 tgcaaagtta ttgtcagata cgattttttt gcacttggga agtagggaaa ggacgacttg
     1141 tggataagac agaaagtgtt aaaagtcaat ttccaaagaa agagaagtga aggaacagtg
     1201 ctgttagttt tactgctgct tttactgagc tgcactgctt ttgcagaagg cgctcaagaa
     1261 gctaccatga ccggctttaa gggctttcgc acccggtctt ctcagatgaa aattacagat
     1321 aatgctctgg tggtcagcaa cgtggcgttt gccgaagcca atacaggaag tcttctttgg
     1381 gaggacttcg atctttcatt caaagtgacc atcggccagc tgggatcgcc gtacgccggg
     1441 tttaccttga tctttagatc ttggggcggg cgggattgct acgcactcac agtagctccg
     1501 gaccggaccg ctgtagaacg cttccacgga gtctacagcg actcgagagt tcttggctat
     1561 gttgcacggg gtatgcgtga cggagaaact gcagattatc gcatcacagc tcaagggaac
     1621 cgcttcaccg tctacaagaa cggtgaaaag attatcgaag ctgtagatcc ggacaacatg
     1681 tacccttacg gctacatggc ggtgcgggcc gagctagcaa gtttgacatt tgcggattgg
     1741 actctgaagg tttccgagat ccgggatccg gttttgcgaa agagagcaga agcgggttat
     1801 ggacttccag ttgagccttt aggaggcgaa atgctaaaag tccaagaggg cgatccccag
     1861 aacatcatgc tcgtctacga ccagtacacc cacaagaact ggatgactgt ggatgcactg
     1921 ccgtatgttg catatctatc cgcaaaaacc tatatgcctc aagattggtt cttcgactct
     1981 ttcctgtttc tggcgctatc tgcaccaggg ggacggaact ttggcggcgg gaaccctgca
     2041 atcttggaag actattactg gtggctggat cggatgttcg ggaaagagaa gttcggcctc
     2101 ggagctttcg aaacggccat cgcaaacgcc aagaatgtgc ttggcgatcc cgaccacaag
     2161 gccaaagtga tcatgatgat tccccacccc agcgagatcg agggcacgcc atttggcgag
     2221 attaacgggg agcgtccggt tctaaggggc aatcctacag aagctgccaa aggtcgcctt
     2281 aaggtggcta agtggtggat tgatgaggtg gtaaagcgct ttaacgagaa gggctatcag
     2341 aacctagaac ttgtgggctt ctactggctg gcagaagatc tagacctcgg cggtattgac
     2401 aagctctatc tacccgatgt gggagattac gtacgctcat tgggtatgaa gttctactgg
     2461 attccctggt acatgagccc ggggtacgac agctggaaag agctggggtt tgattattcc
     2521 atcatgcagc ctaactacat gttcgataag gatatcgcca agaccagatt ggagaagacc
     2581 gctgtgttag cggcagaagc caactgcggt atcgaaattg aagctgatga ctccgtctgg
     2641 acgccaagcg gcaaagaccg gtatctcgat tacctccgag ctgccaacac ccacggctgg
     2701 gcaggcgatg tgatcaaggc cttctaccag gaggtagacc tcttcggcaa ggctgccatg
     2761 agctcttacc ctcccgatcg ggaaatctac gatatcacct acgggtttgt aaaaggcacc
     2821 tggactgaga agctaaagtt tgaagaagaa aactaaggag ctgaggggtc acccctcagc
     2881 tttttgtatc tttgagtatt gcttttactt cgcaggataa accgaggtct atcggggaac
     2941 ttatcgggtt tgattacagt tgttgacatc tactgcagcg ttctatatac cgggtatata
     3001 gatatatact gagtacgtag agataggggg gaggcagcct tggcagtaaa acacgtcttt
     3061 ttggcacttc ttgcaaagca gccaatgcac ggctacgagc tgaaaaacgc ctttgaagag
     3121 attgcagctg gcagctggcc tttaaacttc ggccaggtct accagacatt gaaccgactg
     3181 gaaagagacg gattcatcga aagtttcgag gtggttcaag aagacaaacc ggacaaaaaa
     3241 gtgtaccaga tcaccgaaga tggaagagat caccttaaag agtggctgca gcaggatgat
     3301 cattggaatc tgtttttcga tgaacaagcg ttgcgcttga tggctcttcc ccttgtggac
     3361 tcagcgcttt caggcgagat tctcaaagga tacagaaact tcattctgcg ggttattgca
     3421 ggccttacaa aaatgctgga agaggaaaaa gacacaacat cgacttacta cgccctgctc
     3481 gaaagaaaca ttctgagagc ggaggctgat ctaacctgga tcaacaggat gatcgaaagg
     3541 tgggaaaggc atgcttgagt taaacgggat ttccaaagtc tacggccagg gagcttcgca
     3601 agtagaagct ctgcgcgatg tagaccttaa catctctcca ggtgagttcg tcgcggtgat
     3661 gggaacttcg ggatgcggca aaaccacgct gctgaacatc atcggctgtt tggacactgc
     3721 tacctccggc atctacacgc tgcaggggaa agatgtatcg agactttcct tgaaggacaa
     3781 agctcgggta cggaataaaa cctttggttt tgtgtttcag gcatttaacc tgcttgcaga
     3841 gtatacggtt ttggaaaacg ttacgctacc tcttagatac agtggtgtgc caaagcgtcg
     3901 gtggaaggaa caaggccttg gtgttctaga gagactaggt attgccgatc taagctctcg
     3961 ctaccccgat cagctttctg gagggcagca gcagcgggtc gccatcgcac gtgctctggt
     4021 aaacgacccg gagattatca ttgcagacga acca
//
LOCUS       DAOSDA010000118         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_14022_length_4046_cov_3.354798, whole genome shotgun sequence.
ACCESSION   DAOSDA010000118 DAOSDA010000000
VERSION     DAOSDA010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14022_length_4046_cov_3.354798"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /gene="dnaX"
                     /locus_tag="PLW82_03755"
     CDS             <1..1305
                     /gene="dnaX"
                     /locus_tag="PLW82_03755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOJ46421.1"
                     /translation="DQCESCVAIREGRSLDVFEIDGASNRGIDEIRQLRETVRFRAAQ
                     GKYKIYIIDEIHMLTIEAFNALLKTLEEPPENVVFVFATTEPQKVPATILSRCQRFTF
                     HRISTDIIAEKLVEISREEGVEVDDTALRIVAETAQGGMRDALSLLDQAMAFSSGTVT
                     AELVQEMLGLTDAQALTAFFEALAKGDLQGGLELVRHASSSGKDLIHFMESAMQVARS
                     LLVVSADRSAPLQSGEILLGKDRKSFVKVAEAFDNAYLVGIIETLAEAVQKSKQTADT
                     ELFFDLALVKLIEEKNKSDELNNLRTLVVEQKKKLSALEKQIGEGKIQVAQNYDVVPY
                     WNSTLEKLKSKKDTLEFYAFLVVAEPVALEGPDLYINFPEAYEFHRANVEKNRDLIEK
                     KLSELSGFTVKLHIDKAPGEQTGPNLLAKTQDVFGGTIVKPE"
     gene            1319..1636
                     /locus_tag="PLW82_03760"
     CDS             1319..1636
                     /locus_tag="PLW82_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOJ46422.1"
                     /translation="MNRHMVMKQMEKMQAELIKLQKELKEKTVEATSGGGVVKAVANG
                     NQELVGLEIDPDAIDKDDAEILQDMIVAAVNEALRKAKEMNEEAMAKLYRGFKIPGMP
                     NMF"
     gene            1664..2260
                     /gene="recR"
                     /locus_tag="PLW82_03765"
     CDS             1664..2260
                     /gene="recR"
                     /locus_tag="PLW82_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOJ46423.1"
                     /translation="MHLPKPLARLIKELNKLPTIGPKTAQRLAFYIVRSDYDKVKSLA
                     DALLEVKESIRYCSICGNITDDDPCALCSDPRRDMSTICVVHGAQDIIAMERTREFKG
                     VYHVLNGAISPMDGVGPDQLRIKELLERINSNNIKEVILALDPNVEGEATAMYIARLL
                     KPLGVYVTKIASGMPFGGDLEYVDEVTLSRALEGRREL"
     gene            2380..3153
                     /gene="codY"
                     /locus_tag="PLW82_03770"
     CDS             2380..3153
                     /gene="codY"
                     /locus_tag="PLW82_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HOJ46424.1"
                     /translation="MTTSLLKKTRKINMLLQRAGGDPVNLNSLAEILSDNIDANCYIV
                     NEQGKILGYYLVDHMEAAALKPENSDLYFDVEFNQTMLHITDARSFNKVDDGDILTED
                     GPIKHKFLMVVPIKGRGDRLGNLLLSRSEDDFVEDDYILAEYGATVAALEVLRQKNEE
                     MEEMERKIAAVRIAVDTLSFSEIAAMKKIFENLEGNEGYLVASKIADEAQITRSVIVN
                     ALRKLESAGVIQSRSLGMKGTYIKILNDLLKEEFERRDM"
     gene            3259..3459
                     /locus_tag="PLW82_03775"
     CDS             3259..3459
                     /locus_tag="PLW82_03775"
                     /inference="COORDINATES: protein motif:HMM:NF022165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 family protein"
                     /protein_id="HOJ46425.1"
                     /translation="MKNSSLETYSLKAAIKDALTEWSTAKNFFDQVTEPDLVDYAVLR
                     LEAAEKRVAFLLKKAREEGEPP"
     gene            3456..3977
                     /locus_tag="PLW82_03780"
     CDS             3456..3977
                     /locus_tag="PLW82_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOJ46426.1"
                     /translation="MKLKLLTRAAIIAAVYAAGTILLGSFGYGPIQVRITEALTVLPF
                     FDPAAILGVTLGCFLANVIGGMGPWDMFGGTLITFIAAVLTNRSKNKLVAISWPILLN
                     AFGVSAYLSVIFELPYWYNVGTIFLGQFIAIGLVGYPMMLYLEKNIHILRLDKQGPTD
                     VYSDSAPKKGQSS"
BASE COUNT         1237 a          806 c          985 g         1018 t
ORIGIN      
        1 gatcagtgtg aaagctgcgt tgcaattaga gaaggcagat ctttagatgt ttttgagata
       61 gacggcgcat ctaatagagg cattgacgaa attagacagc tcagagagac tgtaaggttt
      121 agagccgctc aaggcaagta taaaatctac atcatcgacg aaatccacat gctgaccata
      181 gaagctttta acgcgctttt gaagacttta gaagagcctc ctgaaaacgt agttttcgtc
      241 tttgcaacca cagagccaca gaaagtgccc gctacaatct tgtcgagatg ccaacgtttc
      301 actttccaca ggatctccac agacatcata gcagaaaagc tagttgaaat tagcagggaa
      361 gagggagttg aggtggatga tacggcactg cgtattgtcg ccgaaactgc tcaaggaggt
      421 atgagagacg cgctcagcct cctggaccag gcaatggcct tttccagtgg caccgttacg
      481 gctgagcttg ttcaggagat gctaggactc accgatgctc aagcattaac ggcgtttttc
      541 gaagctcttg ccaaaggcga cctccaggga ggtttggagc ttgttaggca tgcaagttca
      601 agtgggaaag acctaattca ttttatggaa agcgctatgc aagtggcccg gtctcttctt
      661 gtggttagtg cagatcggtc tgctccattg cagtctggcg aaatactcct cggaaaagac
      721 aggaagagtt tcgtcaaagt cgcagaggcc ttcgataatg catatcttgt aggaataatc
      781 gagactcttg ccgaggctgt gcaaaagagt aagcaaacgg cagatacaga actatttttt
      841 gatctggcat tggtaaagct aatagaagag aaaaacaaga gcgacgaact gaataactta
      901 cgcaccttag tagtagaaca aaaaaagaag ctttctgcac tggaaaaaca aattggagaa
      961 ggaaaaatcc aagttgcaca aaattatgat gtagttccat actggaatag cactttggaa
     1021 aaattaaaat ctaaaaaaga tacgctggag ttttatgcct tcttggtggt tgctgaaccg
     1081 gtagcccttg aagggccaga cctctatatc aacttccctg aagcctatga gttccacaga
     1141 gccaacgttg agaagaacag ggatcttata gagaaaaagc tcagtgaact ttcggggttc
     1201 actgtcaaac tgcatattga caaagcccca ggtgaacaaa caggccctaa tcttttggca
     1261 aaaacccagg atgtctttgg aggaacaata gtaaaacctg agtaaggagt tgctggtaat
     1321 gaatagacac atggttatga agcaaatgga aaagatgcaa gcagaattaa tcaagctcca
     1381 aaaagagtta aaagaaaaga ctgtcgaagc tacaagtggt ggcggtgtag tcaaagcagt
     1441 tgccaatggc aatcaggaac tggttggttt ggagatcgat cccgatgcta tcgataaaga
     1501 cgatgcggag attttgcagg acatgattgt cgctgctgtc aacgaagcgc tgcgcaaagc
     1561 caaagaaatg aacgaagagg ccatggccaa gctgtaccgt ggttttaaga tccccggaat
     1621 gcccaacatg ttttagacag ttagggttta aggaggaaaa cctatgcatc tgcccaaacc
     1681 tttagcgcga ttgatcaagg aactgaacaa gcttccaacc ataggaccaa agaccgctca
     1741 acgattggct ttttatatcg taagaagcga ttacgacaag gtaaagagcc tagcagatgc
     1801 acttctagag gtaaaagagt ccatacgcta ctgttcgatc tgtgggaata ttactgatga
     1861 tgatccttgt gcgttatgct ccgaccccag gagggacatg tccacaatct gtgtggtgca
     1921 tggcgcacaa gatattattg ccatggaaag gacccgcgaa tttaagggag tctaccatgt
     1981 gcttaatgga gcaatttcac cgatggacgg tgtaggtcca gatcaacttc gtataaaaga
     2041 actcctggag cggattaata gtaataatat aaaggaagta atacttgcgt tggatcccaa
     2101 tgttgaagga gaagctactg ccatgtatat cgcccgtctg ctcaaacctt tgggggtgta
     2161 cgtaacaaag attgcaagcg gcatgccctt tggcggagat ctagagtatg tcgatgaggt
     2221 gaccctcagc agggctcttg aaggcagacg ggaattgtga aatttcagaa atgtactttt
     2281 atctctttcc ggcatgtgtt ataatacact tgatacgaag gaatacttat acgatggttc
     2341 tgaattttcg ttgtaaggtc gctttaagga ggattaaaaa tgaccacttc tcttttgaaa
     2401 aaaaccagaa aaatcaacat gttgctgcaa agagcaggtg gggacccagt aaatctcaat
     2461 tcgttggctg aaatactcag cgataacatt gatgctaact gctacattgt taacgaacaa
     2521 ggtaagatcc tgggctacta cctggttgac catatggaag ctgcagcact caagcctgag
     2581 aacagtgatc tctactttga tgtagagttc aaccaaacca tgcttcacat taccgatgca
     2641 cgttccttta acaaggtcga tgacggtgat attttgacag aagatggccc tatcaagcac
     2701 aagttcctga tggttgttcc cattaagggc agaggcgata gactgggcaa tctccttctg
     2761 agccgttctg aagatgactt tgttgaagat gactatattc tagccgagta cggggcaact
     2821 gtagcagcac tcgaagtgct tcgccagaag aacgaagaga tggaagagat ggagagaaag
     2881 attgctgctg ttagaatcgc agtggacacg ctttccttct ctgagatcgc cgctatgaaa
     2941 aagatctttg aaaacctcga aggcaacgaa ggctatctag tagccagcaa gattgcagac
     3001 gaagctcaaa tcactcgttc ggttatagtt aatgcgctga gaaaattgga gagcgcaggc
     3061 gtcatccaat ctcgctcgct aggtatgaaa ggcacttata tcaaaatact aaacgatcta
     3121 ctaaaagaag agttcgagcg cagagacatg tagaaaccgt tgcaggaaaa tactgcaact
     3181 gcgactatat aaagtataaa agtctctaga aagagataac attaacttga ctgagtaatt
     3241 agcttggagg tcaggtcaat gaaaaactct tctctagaga cttactcttt aaaggctgct
     3301 attaaagatg cgttgaccga atggtccact gccaaaaact tctttgatca agtcaccgag
     3361 cccgatctgg tggactatgc agtgctgcgg ttagaagcgg ccgaaaaaag agttgctttt
     3421 ttgcttaaga aagccagaga agaaggtgaa ccaccgtgaa attgaagtta ctaactagag
     3481 cagctatcat tgcagcagtg tacgcagcag gaacgattct tcttgggtct tttggttacg
     3541 gccctatcca agttcgtatt acggaagccc ttacggtgct cccttttttc gaccctgcgg
     3601 cgattctggg agtgaccctt ggatgctttt tagccaacgt aattggtggc atgggtcctt
     3661 gggatatgtt cggcggcacg ctgattactt tcatagctgc cgttcttaca aatagaagta
     3721 aaaacaaact agtggcgatc tcttggccaa tcctgctgaa tgcctttggg gtaagtgcat
     3781 acctttcggt catattcgaa cttccttatt ggtataatgt agggacaatc tttcttggac
     3841 agttcatcgc cattggtctt gttggttacc caatgatgct gtatctggag aaaaatattc
     3901 acattttaag actggacaaa cagggtccaa ctgatgtata ctcagattca gccccaaaaa
     3961 aagggcaaag cagttaacct tcgctaaaaa aatatcgaaa aagtagttga cgaaggtttt
     4021 atgcggtgat aaaataagat tgttgt
//
LOCUS       DAOSDA010000119         4045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_14026_length_4045_cov_3.715539, whole genome shotgun sequence.
ACCESSION   DAOSDA010000119 DAOSDA010000000
VERSION     DAOSDA010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14026_length_4045_cov_3.715539"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PLW82_03785"
     CDS             complement(<1..939)
                     /locus_tag="PLW82_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ46427.1"
                     /translation="MKLPEFGVKRPVTITMIALGLLLFGFISLTKIGLDFLPEIDIPV
                     TAVVIPYPGADPETVEEQITKVVEPSLRTVSGVNTIMANSLENMAVIVMEFEWGTDLK
                     DASEEIRSLFDMLSYSLPTGASTPMIYKVDMNQIPLMALTVAGGEAIDTSQWVDEVLR
                     PALESVSEVGRIDVNGQPVHQIEVAYYPEKLAEYNISPSVIGQMLAYQNLMIPAGTIE
                     DGGLNYNLRAGYAFTSIEDIESLVVGQAKGEVNPFLGIAIPRMVRLGDVAEVREVFSR
                     EGQISTVNGKPAIVVMVYQASDANAVNASRAILNKLS"
     gene            complement(999..1682)
                     /locus_tag="PLW82_03790"
     CDS             complement(999..1682)
                     /locus_tag="PLW82_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46428.1"
                     /translation="MKKSAKRNIISILAIILSLSVGILALEAVDVVNEALANISKFET
                     VSLELKVETGQGLGGTMVMSVKSHLKEEVGRLEFTSPKELAGQIIIVDKPKDQFINYY
                     PTGQAVRMPLANAQAAGTGFDVSSLMELNVANLDELDLDRFVLGLDETTLDGVPAYVI
                     SVMDTKNEVGLQKIWLRKADKFPIYMESYDLGGNRIMTMTLDKVEINKGLDLAILRAL
                     PKTAVIFDM"
     gene            1947..3110
                     /locus_tag="PLW82_03795"
     CDS             1947..3110
                     /locus_tag="PLW82_03795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOJ46429.1"
                     /translation="MKRVSKLLLICSLLLAILTSALAQSEPLFTVQAKSAVLMDAETG
                     QVLFEQNADEKLLPASLVKIMTLYLTFDAIEKGTVSLEDRFRVSINAWQPEGSKMFIR
                     EGERVSVEDLIKGVAIVSGNDACITLAEGISGVTEVFVRSMNQKAQSLGLTNTYYTDV
                     HGLDNTGAQFTTARDVAMLSRAYVNDHPEALVYHSTREFTYAGITQRNRNGLLWNYPG
                     ADGLKTGHLNAVGYNLVATAKENDMRLIAVVLGADSESVREAEARKLLNYGFRNFETV
                     KASELVKPVNTTVYKGKVGKVNVVPAKDVSMVIPQGAADRVRVVRDLDSALVAPVKKG
                     DPVGEYRFYWQDELIASVEATVAEDVEKGGFFKVLWDSIRLFFANLFAKITNK"
     gene            complement(3290..>4045)
                     /locus_tag="PLW82_03800"
     CDS             complement(3290..>4045)
                     /locus_tag="PLW82_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46430.1"
                     /translation="RRDNFEQKTVEIVLKFKEEVDLDSFLESQIRDKFELEEDVNLCL
                     DVAKVSFRVPGGGEVQNFSLSVLAGEKVQVVSSRAEQMALLEILLGIKRPESGGVSYV
                     DVDQGTISRLRALRQMAYLSVPELQTKSWGLTVREQVGFGDITKVNDDGRILELLKKT
                     GILASSERSERLLDRLTPHGGVEEKKLLHLRALFGGRRFFLVDGSTFDWQVDASLLTE
                     IVQDPRSTVLFFTRERLPLEGFTRVVDLADYTV"
BASE COUNT         1017 a         1017 c          912 g         1099 t
ORIGIN      
        1 gctgagtttg ttcaaaatcg ctcttgaggc gtttacggca ttagcatcac tggcttgata
       61 gaccataaca acgatagcag gcttgccgtt aaccgtactg atctggcctt ctctggagaa
      121 gacctcccta acttctgcca catcgcctaa cctgaccatc ctgggaatgg cgatgcctaa
      181 aaacgggttg acttcgccct ttgcctgccc cacaacgaga gactcgatgt cttcgatgga
      241 agtgaaagca taacctgcac ggaggttgta gttcagtccg ccgtcttcaa tggtccctgc
      301 aggaatcatc aggttctgat aggcaagcat ctggccgatg acagacgggg agatattgta
      361 ttccgcaagc ttttccgggt agtaggcgac ttcgatctga tgcaccggct gcccgttgac
      421 atcaatgcgt ccaacttcgc tgacgctctc aagggccggg cgcaggactt catcgaccca
      481 ctgacttgta tcgatagctt ctccaccggc aacagtcagc gccatgagcg gtatctggtt
      541 catgtccacc ttgtagatca tgggagtgct agcacctgtt ggcagagaat agcttagcat
      601 atcaaaaagg ctgcgaattt cttcagaggc atctttaaga tcagttcccc attcaaactc
      661 catgacgata acagccatat tttcaaggct gtttgccata atggtgttga ctccgcttac
      721 tgtccttaaa gaaggctcta caactttggt aatctgttct tctactgttt ccggatctgc
      781 acctgggtag ggaattacta ctgcggtcac gggaatgtcg atctcgggga gaaagtccag
      841 accgatttta gtaagagata tgaagccgaa aagaagcaag cccagtgcga tcatggttat
      901 ggtaactggt cttttaacgc caaattctgg tagtttcatt tttaaatctt gttcctccct
      961 cgtctaatgg taaaccccgg cgtgcggggt ttttcttgct acatatcgaa gataaccgcc
     1021 gttttgggca gcgctcttag gatcgcaaga tccaggcctt tgttaatctc gactttatcc
     1081 aaggtcatgg tcatgattct atttccaccg aggtcgtagg attccatgta gattggaaac
     1141 ttatctgctt ttctcagcca gatcttctgt agacctactt cgtttttagt gtccatcacg
     1201 gagatgacgt atgcaggtac tccgtccaaa gtggtttcgt caagaccgag aacgaagcga
     1261 tccaaatcca gctcgtcaag atttgctaca ttcagttcca taaggctgga aacatcaaaa
     1321 ccagtccctg ctgcttgggc gttagcaagg ggcatcctca ccgcttgacc tgtggggtag
     1381 tagttgatga actggtcttt gggtttatct acaatgatga tctgccctgc aagttcttta
     1441 ggtgaggtaa actctaagcg cccgacctct tccttgagat ggctctttac agacatgacc
     1501 atggtgccgc ctaacccctg accggtttcc actttaagct ccaatgaaac ggtttcaaac
     1561 ttgcttatgt ttgcgagggc ttcattgaca acgtccacag cctctaatgc aagaatccct
     1621 acacttaagg acaaaataat ggctaagatg ctgataatat tccgctttgc gctttttttc
     1681 attctcatct actcctctca aaataatctg tcccctgcct ctgtaatttg gcttagactc
     1741 ttgggattta ttattgaaaa cagtaaaaac ttgcagggtg gacagtttct ctagtcgaat
     1801 gaagtataag ctgagttttc ccagctgcct gaaacccgca ctagagcttc ttttacaaaa
     1861 actctaatta ggcttacacc tttttcagcc tacaaatgca agttccaata taggttagga
     1921 gggaaggtgc tttatgcacc gatggtatga aacgtgtttc taaactgcta ttaatctgct
     1981 ctttacttct agcaatctta acttctgcac tggcccaatc ggaacccctt tttacggtac
     2041 aggctaagtc ggcggtttta atggacgctg aaactggaca agttcttttc gaacagaacg
     2101 ccgatgagaa gctgcttcct gcaagccttg tcaaaatcat gaccttgtat cttacttttg
     2161 acgctataga aaagggcacc gtttctcttg aagaccgctt ccgcgtgagc atcaacgctt
     2221 ggcaaccaga aggctccaag atgtttatcc gcgaaggcga acgtgtcagt gttgaagatc
     2281 tcataaaagg cgtagccatt gtttccggga acgatgcctg catcactctt gctgagggta
     2341 tctccggtgt taccgaagtt tttgttcgct ccatgaacca aaaagcccaa tctcttggtc
     2401 ttacaaacac ttactacact gatgtccacg ggttggacaa caccggggcg cagttcacta
     2461 cagcaagaga tgtggcgatg ctttcaagag catacgtcaa cgatcaccct gaagcactag
     2521 tctatcattc cactcgcgag tttacctacg ctgggatcac ccaaagaaac cgcaatggac
     2581 ttctttggaa ctacccggga gcagatggac tgaagaccgg tcacctaaat gccgttggct
     2641 acaacctagt ggcaacagcg aaagaaaacg atatgcgcct tattgctgtt gttttaggtg
     2701 cagactctga gtctgtccgc gaagcagaag caagaaagct tttaaactac ggcttccgca
     2761 acttcgaaac agttaaggct tccgaacttg ttaaaccggt aaacactacg gtctacaaag
     2821 gcaaggtagg caaggtgaat gtagttcctg ccaaagatgt ttccatggtc attcctcaag
     2881 gcgcagctga tagggtgcgg gtagtccgtg atctggactc tgcccttgtg gcgccagtga
     2941 aaaaaggcga ccctgttggc gagtaccgtt tctattggca ggatgaactc atcgcatctg
     3001 tagaagcaac agttgccgaa gatgtagaga aaggcggatt cttcaaggtg ctttgggact
     3061 ccatcaggct gttctttgca aatctctttg ccaagatcac aaataagtaa tctcctgaaa
     3121 gatcctatgc tagctccttc cggcaatcgc tccttctctt acaataccgc aaaaccattg
     3181 ggttgttaag atattagtta ggtaacctaa caaaattatc tttcataatc ctggtttctt
     3241 caagtagcaa aaacccccta agttcaaatc ggacttaggg gtttttttct taaactgtat
     3301 aatcagcaag atccaccact ctggtaaaac cttccagagg aagtctttct ctggtgaaaa
     3361 agagcaccgt acttctggga tcctgcacga tctctgtcaa aagggacgca tcgacttgcc
     3421 agtcgaaggt tgaaccgtca acgaggaaaa acctcctccc tccaaataga gctctgagat
     3481 gaaggagctt tttctcctcc acccctccgt gaggagttag ccggtccagc agcctttcag
     3541 atctttcaga actggcaagg attcccgtct ttttcagaag ttccaggatc ctgccgtcat
     3601 cgttgacttt tgtgatgtcg ccaaatccta cctgttctcg gacggtgaga ccccacgact
     3661 ttgtttggag ttcgggaact gacaggtacg ccatctgcct cagagccctc agcctggaga
     3721 tggttccctg gtctacatca acataggaaa ccccgccgga ctcaggtctc ttgattccca
     3781 aaagtatctc caggagtgcc atctgctccg cccttgagga gacgacctgg accttttcac
     3841 cggcaagaac cgaaagggaa aagttctgta cttccccacc tccaggaacg cgaaaagaca
     3901 ctttcgccac gtccaagcag agattcacgt cctcttcaag ctcgaacttg tccctaatct
     3961 gactttctag gaaggagtct aggtccactt cctctttgaa cttcaggacg atttcgacgg
     4021 ttttctgctc gaagttgtct ctacg
//
LOCUS       DAOSDA010000120         4006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_14181_length_4006_cov_4.034169, whole genome shotgun sequence.
ACCESSION   DAOSDA010000120 DAOSDA010000000
VERSION     DAOSDA010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14181_length_4006_cov_4.034169"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLW82_03805"
     CDS             complement(<1..751)
                     /locus_tag="PLW82_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ46431.1"
                     /translation="MSDLKPLLMEHARSVGADLIGFASKERFADVVPEENPFAIFPEG
                     ETVILLGKRITRGALRGVEEGTNFGDYTLFGSAWLDDNFNSLMCYEVTRFLEDRGFEA
                     VPIFPNPTEAKGMGVSVAEGKPAPNVTPDFQLAAVLCGLGEIGYCGEVLTPEYGPRQR
                     WQMIITDAKIEADPILKEPICNQCGKCAEVCPLGAITGIKTVKIGGKKMAVGNIDYNL
                     CRKCKNGAFPNRLLARAKPDRLAALCVKTCIH"
     gene            complement(855..1886)
                     /locus_tag="PLW82_03810"
     CDS             complement(855..1886)
                     /locus_tag="PLW82_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ46432.1"
                     /translation="MTRTTIRDVAKEAGVSISVVSYVLNDNPNVSISRETRERVLAAA
                     KKLNYTPNRIAQSMRTQKSRVLGLATFWDISDSVFTDILKGIDSVAEKHGYAVTYCHI
                     KNTSSADKIIDLYNQRQIDGVIFLLHVDAAKNFTEKNFLKKIKNQQIPAVIINGSTQD
                     EDLSYVYIDYYNTSSVAVEYLYSLGHRKLAYMLPDKDEVNRRQAVQRINGFKAALTDL
                     GLDQYAPFYFDQDTVGELIKILREEDVERKPTAIVAHKTNYAAFLLKALLEAGVKVPD
                     DVSVIALNDNYIADYLTPPLTSVRVPTYSIGQKSAELLFDILKGKILNEKLKLPNKVV
                     ERQSCRPLV"
     gene            2083..2643
                     /locus_tag="PLW82_03815"
     CDS             2083..2643
                     /locus_tag="PLW82_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007130925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HOJ46433.1"
                     /translation="MEKKVLAKEDQERLLQTLKERFEQNMERHKGLTWDSVQARLEVN
                     PAGLWSLHEMERTSGEPDVVGVDEQTGGYIFVDCAKETPSGRTNLCYDDEALESRKKN
                     KPTGSALGMAKAMGIEILTEEEYFKLQELGEFDNKTSSWVYTPQELRKLGGALFGDRR
                     FGRVFIYHNGAESYYSARGFRGKIQV"
     gene            2859..>4006
                     /gene="hcp"
                     /locus_tag="PLW82_03820"
                     /gene_synonym="priS"
     CDS             2859..>4006
                     /gene="hcp"
                     /locus_tag="PLW82_03820"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOJ46434.1"
                     /translation="MNMFCFQCQEAAKGVGCDVRGVCGKSPEVAKLQDLLIYIAKGIS
                     LIVEKGNVDTKGLDELNTEIFSTLFMTITNANFDEAALEKQIRKMLTLRDELAAKTGL
                     SHLHDATSFQVSTREEMLEKASKVGVLATEDEDVRSLREMIIYGLKGMAAYAEHAANL
                     GKKDAEISKFMYEALAKTLDDSLTADDLVALTLKTGEYGVKVMALLDEANTSRFGHPE
                     VTEVSIGVRSNPAILISGHDLNDLEQLLEQTKGTGVDVYTHGEMLLAHYYPAFKKYDH
                     FAGNYGNAWWKQVEEFQTFNGPILFTTNCIVPPRSEEVRKRIFTTGSAGFPGCVHIEA
                     DENGKKDFTAIIEMAKTLPAPRQIESGTIVGGFAHNQVMQLADKVVEAV"
BASE COUNT         1059 a          949 c          987 g         1011 t
ORIGIN      
        1 cgtggatgca ggtttttacg cagagggcag caagtcggtc gggttttgcc ctggcaagga
       61 gtctgttggg gaaagcgccg tttttgcact ttctgcagag gttgtagtca atgtttccta
      121 cggccatctt cttgccgccg atctttacag ttttgatacc cgtgattgct ccaagaggac
      181 agacctcggc gcatttgcca cattggttgc aaattggttc tttaaggatg gggtctgctt
      241 cgatcttagc atcggtgata atcatttgcc atctttgccg tggaccatat tccggtgtca
      301 gcacttcacc gcagtagccg atttctccaa gaccgcaaag gaccgccgcc agctgaaagt
      361 ccggcgtgac gttaggagca ggcttccctt ctgctaccga gacacccatg cctttggctt
      421 ctgtaggatt ggggaagatg ggaacggcct caaagcccct atcctccagg aatcttgtga
      481 cctcgtagca cattagggag ttaaaattgt cgtccagcca agcggaacca aagagggtgt
      541 agtccccgaa gttcgtccct tcttcgactc cacgcaaggc gcctctggtg attctcttcc
      601 ccaggagaat gactgtttct ccttcgggga agatggcaaa tgggttttct tcagggacta
      661 catctgcaaa cctctccttc gatgcaaaac caataaggtc agcccccacg gatcttgcat
      721 gctccatcaa cagtggtttc aaatcgctca taaacatccc cttcccggtt ttttcatcta
      781 tagaaacgtt tctataacgg ttataagact gccttcccca aggggaagac gggcttttgc
      841 ccaaaacaag gcctttatac tagtggtctg caggactgcc gttccactac tttgttcgga
      901 agcttcagtt tctcgttcag gattttgccc tttagaatat caaaaaggag ttctgcgctt
      961 ttctgtccga ttgagtatgt aggcactctg acgctagtta ggggaggagt gagatagtcg
     1021 gcgatgtagt tgtcgtttaa ggcaatcact gaaacatcgt ccggaacttt gaccccggcc
     1081 tccaagagag ccttcagcaa aaatgctgcg tagttggttt tgtgggctac gatggcagtg
     1141 ggctttcttt ctacatcttc ttctcttaga atcttgatga gctctcctac tgtatcttga
     1201 tcgaagtaga aaggagcgta ctgatccagc cccaaatctg tgagagcagc tttaaagccg
     1261 ttgattctct gtactgcctg cctgcggttg acttcgtctt tatcaggcag catgtaagct
     1321 agcttcctgt gtccaaggct atagaggtac tcaacagcta cagacgaagt gttgtagtag
     1381 tcgatataga cgtagcttaa gtcttcgtct tgggtgctcc cgttgataat caccgctgga
     1441 atttgctggt ttttgatttt cttcaggaag ttcttctcgg tgaagttttt cgctgcgtcc
     1501 acgtggagga gaaaaatcac tccatcgatc tgccgttgat tgtacagatc tataatcttg
     1561 tctgcgctgg aagtgttttt gatgtggcag tatgtaaccg cataaccatg cttttcagct
     1621 accgaatcga ttcccttgag gatgtcggta aacaccgagt ctgatatgtc ccagaacgtt
     1681 gccagcccca aaaccctaga cttctgcgtc ctcatgctct gggcgatccg gttgggtgtg
     1741 tagttgagct tcttggctgc agctagaact cgctctcggg tttctctgct gattgacaca
     1801 ttgggattgt cgtttaaaac atacgagacc acagagatgc ttactcctgc ctcttttgcc
     1861 acatctctaa ttgttgttct tgtcactaaa ccaccctgca atctgtagaa acgtttctgc
     1921 tcacagatta caaaaccttt ttttgtttgt caataatcac aagcgcctac agaggccaat
     1981 tagcacgatc gtacagatat ttcacaaaaa ctaaaagaaa gctactaaaa atccgcaatc
     2041 tcgggtaata aaaaaacata attcaacatt gggaggaacg gcatggaaaa gaaagtgctt
     2101 gcaaaggaag accaagaaag actcttgcag actctaaaag agcgttttga acagaacatg
     2161 gaacgccaca agggtcttac ctgggacagt gtacaggcta gactagaggt aaatcccgca
     2221 gggctctggt cccttcacga gatggagaga accagcggtg agcctgatgt agtcggggtg
     2281 gacgaacaga cgggaggtta catctttgtc gattgtgcaa aagaaacgcc gtcaggtaga
     2341 acaaaccttt gctacgacga cgaggctctt gagtcccgaa agaagaacaa acccacaggc
     2401 agcgcgctgg gcatggctaa ggccatgggg atagagatcc tgacagaaga ggagtacttc
     2461 aaactccagg aacttggcga gttcgacaac aaaacgtcca gctgggttta tacgccccag
     2521 gaactcagga agctgggagg agcactcttt ggcgatcgcc gttttggtcg ggtgttcatc
     2581 taccacaacg gtgctgaatc ttattactca gcacggggtt tccgtgggaa gattcaggtc
     2641 taagcgttca gacaaaaacc tacaggaatt tagcaaaaca ccttgatttc tcccctcgtt
     2701 tccattaaaa taaacctcgt aagattatta agtaacttaa ctaactaaat caaaacacaa
     2761 aatccgatgc cttctcaagc cactgtgcag cagactagtt ggaccagttg gctactaaga
     2821 cggtacctgg aaacggatgt acgagggagg aaaagacgat gaacatgttt tgctttcagt
     2881 gccaggaagc agcaaaggga gttggctgcg atgttagagg cgtatgtgga aaaagcccag
     2941 aggttgcaaa acttcaagac ctcttgattt acattgctaa aggcatttcc ctgatagtgg
     3001 aaaaaggaaa tgtcgataca aaggggctcg atgagctcaa tacagagatt ttcagcactc
     3061 tttttatgac tattaccaac gccaatttcg acgaagctgc actggaaaaa cagattagaa
     3121 aaatgttgac gctacgagat gaacttgcag ctaagaccgg cctttcccat ctgcacgatg
     3181 cgacatcttt ccaagtgtct acaagagaag aaatgctcga gaaagcgagt aaggtcggag
     3241 ttcttgccac agaagatgaa gatgttcgct ctctcagaga aatgatcatc tacgggttga
     3301 agggcatggc tgcttacgct gagcacgctg ccaacctcgg gaagaaggac gcggagatca
     3361 gcaagtttat gtatgaagct ctcgccaaga cgctcgacga ttcccttacc gcagacgacc
     3421 ttgtagcact aactctcaaa accggtgagt acggtgtgaa ggttatggcc ctcttagatg
     3481 aagccaacac ctcgcggttc gggcaccctg aggtcacaga agtgagtatc ggtgtacgca
     3541 gtaaccccgc tatcctaatc tccggacacg acctaaacga cttggaacag cttctcgaac
     3601 agactaaagg tactggtgtc gatgtttaca ctcacggtga gatgctgctg gctcattact
     3661 acccagcatt caagaagtac gaccatttcg ctgggaacta cggcaatgcc tggtggaagc
     3721 aggttgaaga gttccagacc ttcaacgggc caatcctctt caccacgaac tgcattgtgc
     3781 cgccgagaag cgaagaagta agaaagagaa tcttcacgac tggttctgca ggtttcccag
     3841 gatgcgtcca tatcgaagca gacgaaaacg gcaagaagga tttcacagcg atcatcgaaa
     3901 tggcaaaaac cctcccggca ccaaggcaga tcgaatcggg aacgattgtc ggaggttttg
     3961 ctcacaacca ggtaatgcag cttgcagaca aagttgttga agcagt
//
LOCUS       DAOSDA010000121         3930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_14558_length_3930_cov_2.816000, whole genome shotgun sequence.
ACCESSION   DAOSDA010000121 DAOSDA010000000
VERSION     DAOSDA010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14558_length_3930_cov_2.816000"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    244..253
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<254..1099)
                     /locus_tag="PLW82_03825"
     CDS             complement(<254..1099)
                     /locus_tag="PLW82_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46435.1"
                     /translation="MPFIGEIELTKKIADANRRLAERNIKLEILPNTFRDYQVQFELM
                     HGGLKKGRGIVYYSPKKQSFRLKVLQTSDEFLEDALDAAFGGEKPKTEVAAYVDGSFA
                     SGTASYGAVILKGGKVVAELSGVLPPDEAASHQIGGELRGALEVLKWAEKNGCSEITI
                     CYDYKGVEKFATGSWKRENPLAERYYQEVRKSRVKVHWQKIAAHTGVKWNERADELAR
                     QAARENPTEAALPRAEQLVQKFEDLLTAQGLEYENKGLFNNQFVRLVVQGKKPVYFDL
                     YDTPKR"
     gene            1221..2255
                     /locus_tag="PLW82_03830"
     CDS             1221..2255
                     /locus_tag="PLW82_03830"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOJ46436.1"
                     /translation="MIQKEFFGKVAGQDVYSYTLTNRGGLSAELLSLGGILNSFLVPT
                     GSGTIDIALGHQSVEDFFSEGALLGGIVGRNANRIKNATFALSGRKIQLEQNDGSHNL
                     HSGSGSYQKKIFTVEEVGKTSLCLKHLDLGEGGFSGTVTVAVTVTLNDQNQLVLEYTA
                     TPSEDTIINLTHHAYFNLSGHLSGSLEDHTFQIFADHYTPVKPDGIPTGEIRPVEGTV
                     FDFTRPRGLDLEQALKDNEVASRRGYDHNFCLKGGREFKDAAAVVSQTTGIKLICSTD
                     QPGLQLYTGNFIAESTPGKGGVYGPRSGFCLETQNFPDAVNNQSFPSPVVRKGEVYRT
                     KTVYEVSLIS"
     gene            complement(2318..3253)
                     /locus_tag="PLW82_03835"
     CDS             complement(2318..3253)
                     /locus_tag="PLW82_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ46437.1"
                     /translation="MMKLTKKLTKKAASHLLFALVVLLALSSLAFAETVTVTHNLGTA
                     TVVKNPEKVVVFDYAILDSLDQMGVEILGLPKSNIPEFLKKFEAPKYINVGTLFEPNF
                     ELIYELEPDVIFISGRQETVYKELAEIAPTVYLAIDTNDYLGSFEKNLTVLGEIFGKE
                     EFVKAEVAKVRSSIEEVSRKVREKGASALILMANDGSLSVYGPGSRFDIIHSEFGFKP
                     ADPNIQVANHGQNISFEYLLKINPEYILVIDRGATVGGSISAQQVMDNVLVRSTDAYK
                     KNRIIYLTSQVWYVASGGLEGTKTMIEDIEKAIEN"
     gene            complement(3270..>3930)
                     /locus_tag="PLW82_03840"
     CDS             complement(3270..>3930)
                     /locus_tag="PLW82_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ46438.1"
                     /translation="IGPNGAGKSTLLSVISRLIKKDAGEVYIDGRELSAWDSKALARK
                     ISILKQSNHINLRLTVRDLVSFGRFPYSEGRLTEEDWFYVDQAISYLKLEEIQHKHLH
                     QLSGGQAQRAYIAMIMAQNTEYCLLDEPLNNLDMQHAVDIMKVLRSMSDDLGKTIVIV
                     IHDINFASCYSDYIVAMKNGVVVSEGTAEEIIDQSILGGLYNLDFHIEEVRDNRICVY
                     Y"
BASE COUNT          896 a         1080 c          943 g         1001 t
ORIGIN      
        1 cgtggtcggt cacaatcaac cacttgagcc cgtacttatc aaagcttaca tgcgaaatat
       61 cgtctagcgt gtgctcgcca tctgaaagaa acgtatgaaa gtgcatttct cctggtaaga
      121 atctgcctgc caaaaaaaca ccctcttgtc cctaagatga tctgctacga ttttttcagc
      181 ttgtcaaaca acctgccgag ttcctcaact aaccccttgt ctcccccgtg aatgtagggg
      241 tcgnnnnnnn nnngcgtttg ggagtgtcgt agaggtcaaa gtagaccggt tttttgccct
      301 gtactacaag gcgaacaaac tggttgttaa aaagtccctt gttttcgtac tccagcccct
      361 gagctgtgag cagatcttca aacttctgca ccagctgctc cgcacgggga agagctgcct
      421 ctgtggggtt ttctctagcc gcttgcctgg caagttcgtc tgccctttcg ttccacttga
      481 ctccagtgtg ggcggcgatt ttctgccagt gcacttttac tctgctcttt cgcacttcct
      541 ggtagtagcg ttcggcaaga gggttctccc tcttccaact gcctgtggca aacttttcca
      601 cgcctttgta atcgtagcag atggtgatct cgctacagcc gtttttctcc gcccacttca
      661 agacctctag agcccctctg agctctccgc cgatttggtg agaagcggcc tcatcgggag
      721 ggagtacccc gctcagctct gcaacgacct taccgccttt aagtatcacc gcaccgtaag
      781 atgcagtacc ggacgcgaaa gatccgtcca cgtacgcggc aacttccgtc tttggttttt
      841 ctccgccaaa tgccgcatct aacgcatctt ccaggaactc atcggatgtc tgcaaaactt
      901 tcagcctgaa ggactgcttc ttcggcgaat agtagacgat tccccgcccc ttctttagtc
      961 ccccatgcat cagctcaaac tggacctggt agtcccggaa cgtgtttggc agaatctcca
     1021 gcttgatatt tctttctgcc aaacgcctgt tggcatctgc gatctttttt gtcagttcaa
     1081 tttctccaat aaaaggcaat caaatctctc cttgctttca cctgctacct gtaatttcct
     1141 tgcaaaatgc gtatctcctg catctttctg ctgcctgctc cagagaggtg caaaggagct
     1201 ctacttagga ggtgcactag atgattcaaa aagagttttt cggaaaagtg gccggacagg
     1261 acgtttacag ctacaccctc acaaaccgcg gcggactgag cgcggagctc ctcagccttg
     1321 gtggtatcct caacagcttt ctcgttccga caggaagcgg taccatcgac atcgccctag
     1381 gccaccaatc cgtagaagac ttcttttcgg aaggagccct tctcgggggc atcgtcggtc
     1441 ggaatgccaa ccgcatcaaa aacgctacct ttgccctcag cgggagaaag atccagctgg
     1501 agcaaaacga cgggtcccac aatctccaca gcggcagtgg ttcttatcag aagaagatct
     1561 tcactgtgga agaagtgggt aaaacctccc tttgcctgaa gcacctggac ctcggtgaag
     1621 gcgggtttag cggcaccgta acagttgcgg tcaccgttac tctaaacgat caaaaccagc
     1681 tggttttaga gtacactgcc accccgagtg aagacaccat catcaacctc acccaccacg
     1741 cctacttcaa cctaagcggt cacctgtccg gaagtctcga agaccatacc tttcagatct
     1801 ttgccgacca ctacactcca gttaaacccg acggcatccc cacaggcgag atccgaccgg
     1861 tagaaggcac cgtgtttgat ttcacaaggc caagaggtct ggacttggaa caagccctaa
     1921 aggacaacga agttgcctcc cgcaggggct acgaccacaa cttctgcttg aagggcggac
     1981 gggaattcaa ggacgctgcc gcggtagttt cgcaaacaac cggcattaag ctgatctgca
     2041 gtactgatca gcccgggctg cagctctaca ccggaaactt catcgcggag tccaccccgg
     2101 gtaaaggcgg cgtgtatggc ccccgcagtg gcttttgcct ggaaacccaa aactttcccg
     2161 atgctgtaaa caaccaaagc ttcccctcac ctgtggtgcg aaagggtgag gtctaccgca
     2221 caaagacagt atatgaggtt agcctaatct cgtaaaaact aaccggaaaa agaaagctgc
     2281 tgcatggcca aattcttgcg ccgtgcagca gctttcttta gttttctatg gctttttcaa
     2341 tgtcttcgat catggttttt gtgccttcaa gtcctccaga tgccacgtac cacacctgag
     2401 aggtgaggta aatgatgcgg ttcttcttgt aagcatcggt gcttcttacc agcacgttgt
     2461 ccatcacctg ctgggcgctg atgcttccgc ctactgttgc gcctctatcg ataaccaaga
     2521 tgtactcggg attaatctta aggaggtatt cgaacgagat gttctgaccg tggtttgcta
     2581 cctggatgtt tggatctgcc ggtttgaagc cgaactcgct gtggatgata tcgaaacgcg
     2641 agccgggacc gtagacgctg agggagccgt cgtttgccat gaggatcagg gcgctggcgc
     2701 ctttttccct aacctttctg ctgacctctt caatagaact ccgtaccttc gctacttccg
     2761 ctttgacgaa ctcttctttg ccgaagatct ctccgagaac ggtgaggttc ttctcgaaag
     2821 aacccaagta gtcgttggtg tcgatggcaa gatacacagt aggtgcgatc tcggcaagtt
     2881 ccttatacac ggtctcctgt ctgcctgaaa tgaagatgac atcgggctcg agctcgtaga
     2941 tcagctcgaa gttgggctca aacaaggtgc ctacattgat gtacttaggt gcctcaaact
     3001 tcttgaggaa ctcggggata ttggatttgg ggagtcccaa aatctcaaca cccatttgat
     3061 ccaaggagtc aaggatggca tagtcgaaaa ccacgacttt ctcagggttt ttaacaaccg
     3121 ttgctgtccc gagattgtgg gtcacggtga ccgtttcggc aaaagctaac gaacttaagg
     3181 ctaaaagcac aaccagcgca aaaagtagat gcgaagctgc tttttttgtg agtttcttcg
     3241 tcaatttcat catcgctaca cctctttagt tagtagtaga cgcatattct gttgtcccgc
     3301 acttcctcga tgtggaagtc caagttgtac agaccgccca aaatgctctg atctataatc
     3361 tcctcagcag taccctcact gaccacgacg ccgtttttca tagctactat gtagtccgag
     3421 tagcatgagg cgaagttgat atcgtgaatc acaatcacaa tggttttgcc gaggtcgtcg
     3481 gacatggagc gcaggacctt catgatgtcc acggcgtgct gcatgtccag gttgttcaaa
     3541 ggttcatcca gaaggcagta ctcggtgttt tgcgccatga tcatggcgat ataagccctt
     3601 tgagcctgcc cgccgctcag ctgatgaaga tgcttatgct gaatctcctc gagcttcaga
     3661 tagctgatgg cctggtctac gtagaaccag tcttcctcgg tgagcctgcc ttcactgtag
     3721 gggaagcggc cgaaactcac caggtccctc actgtcaggc ggagattgat atggttcgac
     3781 tgtttgagga tggagatctt tctggccaga gccttgctgt cccaagccga aagctcccgg
     3841 ccgtcgatgt agacctcgcc tgcatccttt ttgatgagcc tgctgatcac cgaaagcagg
     3901 gtgctctttc ccgctccgtt tggaccgatg
//
LOCUS       DAOSDA010000122         3927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_14572_length_3927_cov_2.636622, whole genome shotgun sequence.
ACCESSION   DAOSDA010000122 DAOSDA010000000
VERSION     DAOSDA010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14572_length_3927_cov_2.636622"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PLW82_03845"
     CDS             complement(<1..998)
                     /locus_tag="PLW82_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOJ46439.1"
                     /translation="MDLAELLKSLSEASGVSGYETMPADLIAERWLPMADEVRKDALG
                     NVIAFKKGKGHGKLLLAAHIDEIGLMVKKIDDNGFIRFATVGGFDFRTLVAQEVTVHG
                     REKLRGVIGAKPPHLQSPSEQKKAYEKDEFYIDVGLKKEKVEELVRVGDIITINREVQ
                     KLSGNRYAGKSFDDRACVVMLTACLERLTEVHHNLDVYLVATVQEEVGTRGAAVSAYS
                     VNPDLAIAVDVCHGEFPGVRADRGFGLGKGPVISMGPDTHPKILEKLKEVADEAKIPY
                     QLEASTSARGTDAWSIQIARNGVACALVSLPLRYMHTSVETLDLADIKEGGRLLAE"
     gene            complement(1163..2350)
                     /locus_tag="PLW82_03850"
     CDS             complement(1163..2350)
                     /locus_tag="PLW82_03850"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOJ46440.1"
                     /translation="MEVSRRAKGIAPSATLAISRKAKELKSQGLSVIDFGAGEPDFDT
                     PEHIVAAACAALGEGFTKYTPTDGIPALKEAIIHKFKQRNNLDYKPDEIIVSCGAKHS
                     LFNAFMCLLDPGDEVILPLPYWVSYDEQIRLCGAKVVPAVPNEELKITGDTIRSLVTP
                     NTKLLVLTSPSNPTGVVYTADELRDIGEAVLETGIGLISDEIYEDFTYGIRAESIVTV
                     VPELKEKTIVVNGVSKTYSMTGWRIGYAAGPRAIIRAMANLQAHSTSNPTSFAQKAAV
                     TAITGPQDDVETMRRAFEERRDVIVAGLSSTDGITCKKPMGAFYVFPNVAGLYGKKTP
                     KGTVISDSQSFSQALLEEALVAVVPGAAFGSDEYVRLSYAASMENIKAGVERIRSFAE
                     SLV"
     gene            complement(2365..2769)
                     /locus_tag="PLW82_03855"
     CDS             complement(2365..2769)
                     /locus_tag="PLW82_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOJ46441.1"
                     /translation="MACFQDDLTVATKHRREIVLITDRVEDIVKKSGVESGICLVFAK
                     HTTCAVTINEAADPTVRQDMLAAFEFISPYLKEYRHMEGNSPAHVLTSLVGPSEVIPV
                     RDGKLALGTWQGIYLCEFDGPRTRRVCVQVLG"
     gene            complement(2775..>3927)
                     /gene="gltA"
                     /locus_tag="PLW82_03860"
     CDS             complement(2775..>3927)
                     /gene="gltA"
                     /locus_tag="PLW82_03860"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOJ46442.1"
                     /translation="KEKNSLPAICGRVCPQEVQCEANCILSKKGKSVAIGALERYVAD
                     KERADGVSVEKPEVTGEQVAVIGSGPAGLTAAADLALLGYKVTVFEALHELGGVLRYG
                     IPEFRLPKSIVDSEIEYIKSLGVTFQTNVIVGVTVSLEDLKAQGYKAFFIGAGAGLPY
                     FLGIPGENLNGVYSANEFLTRVNLMKAYKFPEVDTPVYVGEKVAVVGAGNVAMDSART
                     ALRLGAKEVHIVYRRSREEIPARAEEVKNAEEEGVVFNLLRNPVEILGDEEFRVNGVR
                     CQKMMLGEKDAKGRRAPVPIEGEFEVFDVDMVIVAIGQGPNPLATRSIPGLKTDERGL
                     ILVDENQQTSIPGVFAGGDIVTGAATVIKAMGAGKTAARSIDEYLKSLR"
BASE COUNT          903 a         1119 c          881 g         1024 t
ORIGIN      
        1 aactctgcga gcaacctacc gccttcttta atgtctgcca gatccagagt ttcaactgaa
       61 gtgtgcatat atctcagagg tagggacacc aacgcacagg caactccgtt cctggcgatt
      121 tgaatactcc aagcatctgt tccccgcgcg ctagtcgacg cctcaagctg ataggggatc
      181 ttcgcttcat ctgctacctc tttcaacttc tccaaaatct tcggatgggt gtcaggtccc
      241 atgctgatta caggtccttt gccgagacca aagcccctgt ctgcccttac accggggaac
      301 tcaccgtgac agacatccac agcaatagcc agatcggggt ttacgctgta agcactcaca
      361 gctgcgcctc tagttcccac ttcttcctgt actgtggcaa ccaagtagac atcaaggttg
      421 tggtgcactt cagtcagcct ttccaggcag gctgtaagca tgaccacaca agctctatcg
      481 tcaaaagact ttccggcata cctattgcct gaaagcttct gaacttctct gttgatggtg
      541 ataatatctc ctactctaac cagttcttca actttttctt ttttaagacc cacatcgata
      601 taaaattcgt ctttctcgta tgcctttttc tgttcgctcg gcgactgcag gtgaggaggc
      661 ttggctccga taactccccg gagtttctcc ctgccgtgaa ctgtgacttc ctgggcaact
      721 agagtgcgga agtcaaaccc gccgacagtt gcaaagcgaa taaacccatt gtcgtcgatt
      781 ttctttacca ttagaccaat ttcatctatg tgggcggcca aaagcagctt cccgtgccct
      841 ttgcccttct taaaagcaat tacattgccc agggcgtctt tcctaacttc gtctgccata
      901 ggaagccatc tttcggctat tagatcagct ggcatagttt catatcccga tacgccactt
      961 gcttcggata atgacttcaa aagctcagct aaatccatgc tctcacctcc caaagttgtt
     1021 gcacttatta tattccccga atgccgggga aaacaacgcc aaaaccatcg gcctacctaa
     1081 aacaattcgc ctgcgccgca attttgcctc ttttttccag aggtcttcac aaaaaagagc
     1141 agccgtaagg ctgcaggttg aactaaacta ggctttcggc aaagctccga attctttcca
     1201 ctccggcctt gatgttttcc atgctagcag cataggacag gcggacgtac tcgtcgctac
     1261 cgaaggcagc acctggaact accgcaacaa gggcttcttc cagaagtgct tgggaaaaac
     1321 tctgactgtc ggagatcact gtgccctttg gggtcttttt gccgtaaagt ccggccacat
     1381 taggaaagac gtagaaagct cccatgggct tcttacaggt gatgccgtca gtactgctga
     1441 gcccggccac aatgacatct cttctctctt caaaagcccg gcgcatggtc tccacatcgt
     1501 cttggggacc ggtgatggcc gttactgctg ctttttgagc aaaagatgta gggttggaag
     1561 tggaatgggc ctgcaggttg gccatggctc tgatgatcgc tctcggtccc gctgcataac
     1621 caatacgcca cccggtcatg ctgtaggtct tggagactcc gttgaccact atcgtttttt
     1681 ctttaagttc gggaacaact gttacgatgg actcggctct aatgccgtag gtgaaatctt
     1741 cgtaaatctc atcggaaatc agaccgatgc cagtctctag aactgcttcg ccgatatctc
     1801 tcagttcgtc agcggtgtac actacaccgg tggggttact tggcgaagta agcaccaaaa
     1861 gcttggtgtt tggagttaca agacttctga tggtatcacc ggtaatcttc agttcttcat
     1921 taggtacggc gggaaccacc ttggcaccgc acaaccggat ctgctcgtca tagctaaccc
     1981 agtacggaag aggcaggatc acttcatctc cgggatcgag aagacacata aaggcgttaa
     2041 acagggaatg ctttgcgccg caggagacga taatctcatc gggtttgtaa tcaaggttat
     2101 tgcgctgttt aaacttgtga atgatcgcct ctttcagtgc tggaattcca tctgttggag
     2161 tgtacttagt gaacccttcg ccgagagccg cgcacgctgc ggctactata tgttccggcg
     2221 tatcaaaatc cggctctccg gcgccgaaat cgataacgct aagaccttgg cttttcagct
     2281 ccttggcctt tctgctgatg gccagagttg ccgaaggagc aatgcctttt gccctccttg
     2341 atacttccat gttctcacct cattctaacc taatacttgc acgcagaccc ttctagttcg
     2401 cggaccgtca aactcgcaga ggtagatgcc ctgccaagtg ccaagagcca acttcccgtc
     2461 ccgcacagga atcacctcag atggtcctac aaggcttgta aggacgtgtg caggagagtt
     2521 tccctccata tgcctgtatt ccttgagata ggggctgatg aattcaaaag cagcgagcat
     2581 atcctgcctg acggtaggat cggcggcctc gttgatcgtc actgcacacg tggtgtgttt
     2641 ggcaaagacg agacagatcc cgctttcgac tccgctcttt ttgacaatat cttccacccg
     2701 atcggttatg aggacgatct ctcttctgtg cttggtggca acagtaagat catcctgaaa
     2761 acatgccatc tttttcacct caaagactta agatattcat cgatggacct ggcagctgtt
     2821 tttccagctc ccatagcttt aatcaccgtt gcagctcctg taacgatgtc gcctccggca
     2881 aacacccctg gaatggacgt ttgctggttt tcgtccacca aaatcaatcc tctctcatcg
     2941 gttttcagtc ccggaatact gcgcgtcgcc aggggatttg gtccctgacc tatagccaca
     3001 atcaccatat ctacatcgaa aacctcaaac tcgccttcga tgggcaccgg agccctcctg
     3061 ccttttgcat ccttctctcc tagcatcatc ttctgacacc tgacgccatt taccctgaac
     3121 tcctcgtctc cgaggatctc cacaggattt ctgagtaggt tgaagactac gccctcttct
     3181 tcggcattct ttacctcttc agctcgcgcc gggatctcct cacggcttct gcgatagaca
     3241 atgtggacct cctttgctcc caatctcagt gcggttctcg cagaatccat agcaacgtta
     3301 ccagcgccga caaccgcaac cttctcaccg acgtaaaccg gagtgtccac ttcaggaaac
     3361 ttgtaggctt tcatcaagtt gaccctagtg agaaactcat tggcagaata cacaccgttg
     3421 aggttttcac cgggtatgcc caagaagtac ggaaggcctg cccccgcacc aataaagaag
     3481 gctttgtagc cctgagcttt taagtcttcc aagctcaccg taactcccac gatcacattg
     3541 gtttggaagg taacaccgag gctcttaatg tactcgatct ctgagtcgac gatggacttg
     3601 ggcagcctga actcagggat gccgtagcgc agaacgccgc cgagctcatg taaagcttca
     3661 aacaccgtaa ccttgtagcc taacagggca aggtcggcag cagcggtcag tcctgcaggt
     3721 cccgatccaa tcactgcaac ttgctctccg gtaacttctg gtttttccac agacactccg
     3781 tctgcccgtt ccttgtcagc aacatacctt tcaagagctc caatggccac agacttaccc
     3841 ttcttactga gaatgcagtt agcctcgcac tggacctcct ggggacagac cctgccgcag
     3901 atggcaggca gagagttttt ctccttg
//
LOCUS       DAOSDA010000123         3887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_14775_length_3887_cov_3.114562, whole genome shotgun sequence.
ACCESSION   DAOSDA010000123 DAOSDA010000000
VERSION     DAOSDA010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14775_length_3887_cov_3.114562"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PLW82_03865"
     CDS             complement(<1..665)
                     /locus_tag="PLW82_03865"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOJ46443.1"
                     /translation="MSSIGSLKKTARERIRSFYWAAFTVNNIANFYNVYNWIGSLSRL
                     LTGSKGSAITSLVPAFADLDLILCLNLALYPALVLGSSTFFLKLIRGAEVRVREVFGG
                     FRKLFKALGLFFALLLTIGVPVILIVGFRAVVGNWLGLGLGFRAIVGSVAAVAAFFLF
                     LDYAVVFYVLAENPNLSVLQVMERSRSIMNGRKPEYALLGLSFCGWILLDLFLLFGLV
                     RIFV"
     gene            complement(720..1379)
                     /locus_tag="PLW82_03870"
     CDS             complement(720..1379)
                     /locus_tag="PLW82_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46444.1"
                     /translation="MDARPDQGEFVYTLIEYRLVDLPWVWIICWVLAPVLFLGCANYF
                     LKLVRGEDVHFFIVFSRFHLFFKAIGLALCLFLLIGLAYGVNYLFVLSINRMFGLGHE
                     FILPILLLSFILPLVVLGRCGMAFFVLADRPHLTLSQMLRESAAISSGVKNNESLASV
                     LFFLGFTSNAEAKTLAILFYPYVFEILASFYVKVTGLGRIGEGSNQTANSVKQSSSQE
                     H"
     gene            complement(1650..2447)
                     /locus_tag="PLW82_03875"
     CDS             complement(1650..2447)
                     /locus_tag="PLW82_03875"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOJ46445.1"
                     /translation="MRSRVELKRIAKDRLRDAYWPSFAVIAITFFAQTVQCLKNIYVA
                     IARPGMFFTVQPYGPAPDIALTWENLSLLVQSAARQFDWVEFLFGIVGPVLMVGCAAY
                     FLKLLRGQDAKVQEVFGYFHLFFRALGVSILVALICGGVFLLGFLAGYLFFWIIGLRF
                     LGMAFAPLIAAIPAVVVSLGYSMVSFVMADNPNLTALEVMNESRKIMSGEKGTLFVLL
                     LSFLGWIILSALFRGLLTIFIYPYFIATITGFYIDITNPAQLSSINL"
     gene            2691..>3887
                     /locus_tag="PLW82_03880"
     CDS             2691..>3887
                     /locus_tag="PLW82_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOJ46446.1"
                     /translation="MINTEKLRQVLALYKKDHAWYFAKEKYKWETVKHFHVHWNINAP
                     VFSDMFAKATELTGNLLASRYYFPRKMILRFCDADEETVRGMFLDLYDESRDLTLRVE
                     KFIAEADTLKEKYTAGKWDQHYQNINSISTYLWLRYPDKYYIFNHNVLADVAKCLENP
                     LPLKRSAGTANLLSGFELYDEIRREVAKDNELVELLQVSLDENCHPDESLRTLTMDIG
                     YYISTVYLKNAKNQSKWFPSDYEPNISVETWVELLQNDEVFTESSLEIMKRMKDIGGE
                     ATCKQLSEKYGETPNFYNAGSSSLARRVAKFTNCPVPTKNTENSKWWPILYVGRYSDD
                     KTKGVYIWKLRNELSEALDRVDLSRIKLYAASSENRTTINYWWLNTNPKVWSFSDLST
                     GSIQYFT"
BASE COUNT         1161 a          967 c          881 g          878 t
ORIGIN      
        1 acaaagatcc tgacgagtcc aaacagaagg aacaggtcca agagaatcca accgcaaaaa
       61 gacagcccaa gaagcgcata ctccggtttt cttccgttca tgatgcttct gcttcgctcc
      121 atgacctgaa gcactgatag gttcgggttt tcagcaagga cgtagaatac caccgcataa
      181 tctaagaaca agaagaaggc cgccaccgct gcaacagaac caacgattgc gcggaatcca
      241 agacctagtc ccagccagtt tccaactact gctctgaagc cgacaatgag gataaccggc
      301 acccctattg ttaaaagtag cgcaaagaat agcccaagag ctttgaacaa cttgcggaac
      361 cctccaaaga cctccctcac gcgaacttcc gctcccctaa tcagcttcag aaaaaacgtt
      421 gagctcccca gtacaagagc tgggtacagc gctaagttta agcacaagat tagatcgaga
      481 tcggcgaagg caggtaccaa ggaggtgatc gcacttcctt tggagcctgt tagaagccta
      541 cttagacttc cgatccagtt gtagacgttg taaaagttgg ctatgttatt gaccgtaaaa
      601 gcagcccagt agaaagaacg aatgcgctct cttgcggtct ttttcaagct gccaatggaa
      661 ctcatctcta ttccatctcc tctgcatcct cgctcagaag attgcttccc gatccagtgc
      721 tagtgttctt gggagctact ctgttttacg gaatttgcag tttgattgct tccttctccg
      781 atgcggccta aaccggtgac tttaacgtag aagctcgcaa gaatctcgaa gacatacgga
      841 taaaacaaaa ttgcaagtgt tttcgcttca gcgttggacg tgaagccgag gaaaaagagt
      901 acactagcta aagattcgtt gtttttcact ccactggaga tcgctgcact ttcccgtaac
      961 atctgtgata gcgtaaggtg tggcctgtct gcgagaacga aaaaggccat tccgcatctg
     1021 ccaagcacaa ccagaggaag gataaaagaa agaagcagaa tcggtaggat aaactcgtgg
     1081 ccgagcccaa acatacggtt aattgatagt acgaaaaggt agttaacccc gtaagccagt
     1141 ccgatgagga gaaacaggca aagtgcaagc ccaatagctt tgaagaaaag gtgaaagcgg
     1201 ctaaaaacaa tgaagaaatg gacatcttct ccgcgcacaa gctttaagaa gtagttggcg
     1261 cagccaagaa aaaggactgg agcaagaacc cagcagatga tccataccca ggggagatct
     1321 acaagtctgt actctataag tgtgtaaaca aattcaccct ggtccggcct tgcatccatg
     1381 ctcgctctac cgggatgaaa cccagttcct tcagtcgctt aagccaaacc tccaacagat
     1441 caaagaactg gtgaaggaag ggcattattg cgaccacaaa agtcgggaaa tacgctttgc
     1501 ggacgtagcc taaatctaag tccagtttta ctttttccgc catgtcgttt cttccacctc
     1561 cagatagaga gcttggattt tctgaacttt cccagcactt tgctgcagac agagtcctca
     1621 aggttagctt tgcctgtatc caaattatct tacaagttaa tagaactcag ctgggccggg
     1681 ttggtaatat cgatgtaaaa accggttatc gtggcgataa agtagggata gataaagatt
     1741 gtaaggagcc ccctaaaaag cgcacttaag atgatccatc ccagaaatga gagaagcaac
     1801 acaaacaatg tacccttttc tccgctcatg atctttctac tttcattcat aacctcaagt
     1861 gctgtgagat tcgggttgtc agccatgacg aaagacacca tggagtaacc aagagaaacc
     1921 acaactgctg ggatcgccgc gatgagcggt gcaaacgcca ttccaagaaa ccttaagccg
     1981 atgatccaaa agaacagata cccagcaaga aagcccaaaa gaaacacgcc tccacagatt
     2041 agtgcaacca aaatagaaac gcccagtgct ctaaaaaaca agtggaagta gccgaacact
     2101 tcttggactt ttgcatcctg tccgcggaga agcttcagga aatacgcagc gcaccccacc
     2161 ataagtacgg gaccgacgat cccgaagaga aactctaccc agtcgaactg tcttgcagct
     2221 gactgaacaa gaagacttag attctcccat gtgagagcga tgtccggtgc tggtccatac
     2281 ggttggaccg taaagaacat cccaggtctg gcaatcgcga cgtagatgtt ttttaggcat
     2341 tgtacagtct gggcaaagaa ggtaatggca attaccgcaa aagacggcca gtatgcgtcg
     2401 cgcaaacggt ctttagcgat acgtttaagc tcaactctcg atctcatgtt ggtacctcct
     2461 ctgtttcaaa ttactggctt taacctgtcc aggtgactgc aaatccgaac ctctgcatcg
     2521 gtcatttctt tggagatata gattcagcag tcctgaagaa actccttcct gatacttctt
     2581 ccgcagatct tctcagctcc tcaagtctac gcctccaaac agcgcgggaa ggtttttttg
     2641 cattacctac gaaagttttt tgaaggcaaa agcggaaagg ggtagttttt atgatcaaca
     2701 cggaaaaact gcggcaggtt cttgccctct acaaaaaaga tcatgcttgg tacttcgcca
     2761 aagaaaagta caagtgggag accgtcaagc atttccatgt ccactggaat atcaatgccc
     2821 cagttttctc ggacatgttt gccaaggcaa cggaactaac aggtaacctg cttgcttccc
     2881 gatactactt cccgcgaaag atgattctgc gcttctgcga cgccgatgaa gaaactgtca
     2941 ggggcatgtt cctggaccta tatgatgaaa gcagagacct taccttacga gttgagaagt
     3001 tcatagcaga agccgatacg cttaaagaga aatataccgc cggcaaatgg gatcagcact
     3061 atcaaaacat aaactccata agcacctacc tgtggctacg ctatcccgac aagtactata
     3121 tcttcaacca taacgtttta gctgatgttg ccaagtgcct cgaaaatccc ttgcccctca
     3181 agcggagcgc aggtactgct aaccttctaa gcggctttga actatacgac gagattcgcc
     3241 gagaggttgc caaagataat gaactagtgg aacttctgca ggtgtccctt gatgaaaact
     3301 gccacccaga tgaatcgctt agaacactga cgatggatat cggatactac ataagcaccg
     3361 tctacctaaa aaacgccaaa aaccagtcga aatggttccc ttcggattac gagccgaata
     3421 tttcggtgga gacgtgggtt gaacttctgc aaaacgacga ggtgtttacc gaaagcagcc
     3481 tcgagattat gaagcgcatg aaggacatcg gcggagaggc cacctgcaaa cagctgagtg
     3541 agaagtacgg tgaaactccc aacttctaca atgccggttc ttcgtcgctg gcccggagag
     3601 ttgccaagtt cacaaactgc cccgtaccta ccaaaaacac tgaaaattcc aagtggtggc
     3661 ctattcttta cgtaggtcgg tactcggacg acaagactaa gggggtgtac atctggaagc
     3721 tccgcaacga gctcagcgaa gcactggaca gggtagattt aagccgaatc aagctttatg
     3781 cagcatccag tgagaaccga accacaatca actactggtg gctgaatacc aatcccaagg
     3841 tttggtcttt tagcgacctc agcaccggaa gcatccagta cttcact
//
LOCUS       DAOSDA010000124         3883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_14796_length_3883_cov_3.326803, whole genome shotgun sequence.
ACCESSION   DAOSDA010000124 DAOSDA010000000
VERSION     DAOSDA010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14796_length_3883_cov_3.326803"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLW82_03885"
     CDS             <1..350
                     /locus_tag="PLW82_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOJ46447.1"
                     /translation="LDAYRDVLKRYRLDSIKDLPDKPDGSEIVIAGIVTKVNLYADRK
                     GQTMAFVDIEDYLGSVNVTVFSELYAKHRPEVGQVFFIKGILQFSSFGDQEEVKVVAR
                     NLLPIEELATRKK"
     gene            complement(455..1000)
                     /gene="nrdG"
                     /locus_tag="PLW82_03890"
     CDS             complement(455..1000)
                     /gene="nrdG"
                     /locus_tag="PLW82_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOJ46448.1"
                     /translation="MNSRAPLSNYEVRVAGIVPESYVDGPGVRFTIFVQGCPHHCSGC
                     HNMDTWDFFGGTSFAVDELLEQIAFAKKENPFLQGVTLSGGEPFSQANVLVHLAKGIK
                     ELGIDLIAYTGYTIEDLPSVPCALELLEYVDVLIDGPFVQELRSLTHPFVGSTNQRII
                     ERKDIIDFLKHIEAEKISSAL"
     gene            complement(1109..1792)
                     /locus_tag="PLW82_03895"
     CDS             complement(1109..1792)
                     /locus_tag="PLW82_03895"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOJ46449.1"
                     /translation="MITKLNPKSPAVEAFKLLRTNIMYASADMPIQVIGCTSSGPTEG
                     KSSSAANLAIAMAQSGSSVLLIDSDLRRPSQHKIFGIHRRPVGLTEVLTGVKEVEECI
                     HDTDVEGLKLLPSGAIPPNPSELLGSRKMKELMQKLRGAFSTIICDLPPVISVTDALV
                     VSPLIDGYLLIVASGSTDRRAAQTAKELLENAKCKILGAVLNRADPQKSYGSYYSHYY
                     YYYQDDSEA"
     gene            complement(1812..3230)
                     /locus_tag="PLW82_03900"
     CDS             complement(1812..3230)
                     /locus_tag="PLW82_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HOJ46450.1"
                     /translation="MELIEYINILKKYWYLIVGLTLVAAVIAGVLSSIMPPIYEADAR
                     ILIREASEAGITSLFGDLTGQSRNQIQNSVEILKSRTLAYNAAEILGLDYALGTPELN
                     GFRKKFSVQPVQGTDFIRITYQSEDPLFAMQAVNALVDAFIESSLVKSREEARAARDF
                     IEDQLVTLEAKLKDVENRLSEFKGTEKVYNPSEEAKAIINKVTNFDVARSEAIVSKRE
                     LEAKLDDLFLQLEKETNTYISSQVIGTNPLVTNIKTQLIAKETELQVLLTKSTENSRE
                     VQNLQKEIETLKAQLNQQVEKIVTSQTETTNPLYQQLIASISQAQSSLTALEARISAL
                     TELIKETEIEFDALPEKEMELARLTRDYQVTESIYTMLLQTREEYRIQEAMQTANIQV
                     IDRAIVPNSPVSPRKLMNVAVASFLGLFLAAMLAFLFEYLDNTLVTKQDVEEYLELTV
                     LAAIPEIASGGDMRGSKKKKTK"
     gene            complement(3362..3541)
                     /locus_tag="PLW82_03905"
     CDS             complement(3362..3541)
                     /locus_tag="PLW82_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46451.1"
                     /translation="MKVRDIILWTLFGGIAGLLFGSLLGGRMWWGIVIGLVLGFGVSM
                     LTWYRRKKEEERYKV"
     gene            complement(3673..>3883)
                     /locus_tag="PLW82_03910"
     CDS             complement(3673..>3883)
                     /locus_tag="PLW82_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOJ46452.1"
                     /translation="RNVLAVLDELSLKYDLERVNDLTSMVKYGCLEAPGLVINEKLVS
                     QGIVLTREQIRELLTNHDSSVRDSE"
BASE COUNT          998 a          879 c          887 g         1119 t
ORIGIN      
        1 ctcttgatgc ttaccgggac gttttgaaga gataccgcct ggacagcatc aaagatctcc
       61 cagacaagcc tgacgggtct gagatcgtca ttgcaggtat cgtgaccaag gtgaatctct
      121 atgcagatcg caaaggacag accatggctt tcgtcgatat cgaggactac cttggatctg
      181 tcaatgtgac cgtgtttagc gaactctatg caaaacacag gccagaggtc gggcaggttt
      241 tcttcatcaa gggtattctg cagttttcaa gcttcggcga ccaagaagaa gttaaagtgg
      301 tagctagaaa tcttcttcct atagaagaat tggcaacccg taagaaataa agaaaaggct
      361 gtgttgcttc aaacacagcc tttttagcac taattcactt ttcagttaaa taaaaaatag
      421 agataacctc cgttagtgga ggcttggaaa tgctttacag ggcggaagag atcttctcgg
      481 cctcaatatg tttaaggaaa tctatgatgt cttttctctc gatgatgcgc tggttggtag
      541 agcctacaaa aggatgggtt agactcctaa gttcctgaac aaaagggccg tcgatcaaga
      601 catctacata ctcgagaagc tccaaagcac aaggcacaga cggcaggtcc tcaatggtat
      661 agccggtgta ggcgatcagg tcaatcccta actctttgat gcctttggca agatggacca
      721 agacgttggc ctgggaaaac ggttcgccgc cgctgagggt aaccccttga aggaatgggt
      781 tttctttttt ggcgaaggcg atctgctcta gcagttcatc tacggcaaag gaagtcccgc
      841 cgaaaaagtc ccaagtatcc atattgtggc agccagaaca gtgatgggga cagccctgta
      901 caaagatggt aaaacgtaca ccaggtccgt ctacataact ttcaggaacg attccagcaa
      961 ctcttacctc gtagttggaa aggggcgctc gggagttcat agtgacacct cttcagtttt
     1021 ccctgatgtt attatcaact agtctaccac agattttcct taaatgctat aaaaagggct
     1081 gcacttcgat tgaagtgcag cggccatttt aagcttcgct gtcatcttga tagtagtaat
     1141 agtagtgtga gtaataggaa ccgtatgatt tttgcggatc agctctattg agaactgcac
     1201 caagaatctt gcatttagcg ttttctagca attctttggc ggtttgagct gctcttctgt
     1261 ctgtagagcc actggccacg atcaacaggt agccatcaat caacgggctt acaacgagag
     1321 catcagtcac ggaaatcaca ggagggaggt cgcagatgat ggtagagaag gctcctctga
     1381 gtttctgcat tagctctttc atcttcctag aacctaagag ttctgaagga ttcggaggaa
     1441 tcgcacctga aggcaggagc tttagaccct ctacatcagt atcatgaatg cattcttcta
     1501 cctctttgac acctgtgagc acttccgtta agcctacagg tcttctatgg attccgaaaa
     1561 ttttgtgctg tgaaggcctg cgaaggtcgg agtctataag aaggacggaa ctgccgctct
     1621 gtgccatagc aatggcaagg ttagccgcgg aagatgattt tccttcagtt gggccggaag
     1681 aagtacagcc aataacctga ataggcatgt ccgcactggc gtacatgatg ttcgttctga
     1741 gaagtttgaa cgcttccact gccggagact ttgggtttag tttggtgatc aaaatgtttc
     1801 cgttgtcgga tctactttgt cttcttcttc ttcgagccac gcatatcacc tccgctagcg
     1861 atttctggaa tagctgcaag aacagtgagt tctagatatt cttcaacgtc ctgcttagtg
     1921 acgagagtgt tgtcaaggta ttcaaacagg aaggccaaca tagctgcgag gaacagaccg
     1981 aggaaggatg ccacagctac gttcatcagc ttccttgggc ttactgggct atttggaacg
     2041 atcgccctgt cgataacctg aatatttgct gtctgcattg cttcttgaat tcggtactct
     2101 tctctggtct ggagaagcat cgtgtagatg ctctctgtta cctgatagtc gcgagtaagt
     2161 cttgctagct ccatttcctt ctcaggaaga gcgtcgaact cgatctcggt ttccttgata
     2221 agttcggtta gggccgaaat tcgagcttcg agagcagtca gactggattg agcttggctg
     2281 atagaagcaa ttagctgttg gtatagtggg tttgtggttt cggtctgaga cgttactatc
     2341 ttttcaacct gctgattcag ctgagctttt aatgtctcga tctctttctg caggttctgt
     2401 acttctctgc tattttccgt gctcttagtc agaagtacct ggagctcggt ttctttggca
     2461 attagttggg tttttatgtt ggtaaccaga gggtttgtgc caatgacttg agacgagatg
     2521 taggtgttag tttctttctc gagctgcagg aacagatcat ctagttttgc ttctagttcg
     2581 cgcttagaga cgatcgcttc actacgagca acatcgaagt tagttacttt gttgataatc
     2641 gctttagctt cttcgcttgg attgtagact ttttcagttc ctttgaactc gctcaagcga
     2701 ttttcaacat ctttaagctt tgcttctaaa gttaccagct gatcttcgat gaagtcgcga
     2761 gcggcacgag cttcttcacg agacttgacc aatgaagact ctataaaagc gtcaaccaga
     2821 gcgttgacgg cttgcatggc aaacagtgga tcttcactct gatatgttat tctaatgaaa
     2881 tcggtccctt ggactggctg aacagaaaac ttctttctaa atccgttgag ctcaggagtt
     2941 ccaagtgcat aatcgagacc gagtatctcg gccgcattgt atgctaaagt gcggctctta
     3001 agtatttcga cgctgttttg aatctggttg cgcgattggc ctgtcaagtc tccgaatagt
     3061 gacgtgatac cagcttctga tgcttcccta atgagaattc tagcatctgc ttcatagata
     3121 gggggcatga tcgagctaag tacacctgca attactgcag caacaagtgt tagacctaca
     3181 ataagatacc aatatttctt tagtatatta atgtattcta ttagttccaa agcaaacatc
     3241 cctcctgatt tgtgccagtc cttaattaag ttcaacacac tatttgacta tccttctctt
     3301 tatttcctat cggttctttt attttcagta aacgtaaagg cggcccgcga gggccgcctt
     3361 atcagacttt gtatctttct tcctctttct tgcgccgata ccatgtgagc atcgagacgc
     3421 caaaaccaag aactaagccg atgactattc cccaccacat acgaccacca agtagggaac
     3481 caaacagcaa gccggcaata cctccgaaca acgtccaaag aataatgtca cgtactttca
     3541 accagttccc ctccctttat tcagttgagg taaaaagcca cccactttct accctgtaca
     3601 tattatagca tattttccca aaaaacctga gggaatcttc tgcgttttag gtaaaaaaaa
     3661 cagaagagag actcactcgg agtctctcac tgaactatcg tggtttgtta gaagttctct
     3721 aatttgctcg cgggttaaga caataccttg agaaacaagt ttctcgttga tcaccaaccc
     3781 gggcgcttcc aggcagccgt atttgaccat gcttgttaaa tcgttaactc tttccaaatc
     3841 gtacttaaga ctcaattcgt ctaataccgc gagcacgttc cgc
//
LOCUS       DAOSDA010000125         3865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_14883_length_3865_cov_3.497375, whole genome shotgun sequence.
ACCESSION   DAOSDA010000125 DAOSDA010000000
VERSION     DAOSDA010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14883_length_3865_cov_3.497375"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PLW82_03915"
     CDS             complement(<1..1255)
                     /locus_tag="PLW82_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ46453.1"
                     /translation="MSNNRTRDFTTGSILKHLVAFSWPMFLGNFLQALYNTVDTFWVG
                     RFLGTEALGAVSASFPIIFFLVALAIGFTISTTILVSQYYGAKDQEGVSRVINTSIFL
                     MVISSIIVTTVGLLFYKQIFRLISLPESLVKPAGEYLVIILGGIIFAFFYNLLSAIMR
                     GFGDSLTPLYFLAIAAVINIILDPLFILGIGPIPAMGIAGVAYATVIAQAISVVFGLI
                     YLFKKAKIATWDRSLIKPDGFILKQLITIGTPAGIQQALVSIAMLFLSSLVNRNGEIV
                     VAAYGLGGRIDQFAFMPAQSVALAVSALVAQNLGANKPERVREVWRYSNLLTTAIAAS
                     ITVAIWFFPDFWARIFTTDLAVREMTVTYLRIVSLSYVVYSLMFTTGGLCRGAGDTFI
                     AMGVSLLSLWLIRLPLATFLSNTMQM"
     gene            1487..1900
                     /locus_tag="PLW82_03920"
     CDS             1487..1900
                     /locus_tag="PLW82_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOJ46454.1"
                     /translation="MQINVRWNGKRQFIGECPSGHELLLDSSPEVGGENAGPRPTEAL
                     LTALGGCTGIDVIMILEKMQQKVTAYHMEITGERNATEPKYFTDIHIKYVITGENLSE
                     DRVKRAIDLSYSKYCSIIHSLKANITTSYEIIEAK"
     gene            complement(2035..3651)
                     /gene="groL"
                     /locus_tag="PLW82_03925"
     CDS             complement(2035..3651)
                     /gene="groL"
                     /locus_tag="PLW82_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174759.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOJ46455.1"
                     /translation="MAKQLLFGEEARRKLEKGVNTLADAVKVTLGPKGRNVVLDKKFG
                     SPTITNDGVTIAREIELEDPFENMGAQLVKEVATKTNDIAGDGTTTATVMAQAMIREG
                     LKNVAAGANPMMMKKGIEKAVADAVEAIRSIAKPIEDKESIAHVAAVSAGDEEIGRLI
                     AEAMDKVGKEGVITVEESQTFGTTLESEEGMQFDRGYVSPYMVTDTERMEAVLEDPFI
                     LITDKKINVIQEILPVLEKVLQTGKPLLIIAEDIEGEALATLIVNKLRGTLNVVAVKA
                     PGFGDRREAMLGDIAVLTGGQVISEKAGMKLENANLNLLGRADKVRVTKENTTIVGGK
                     GAQEAIKGRITQIKAEIEESTSDYDKEKLQERLAKLAGGVAVIRVGAATETELKEKKH
                     RIEDALSATRAAVEEGIVPGGGVTLIHAAKALEKTENEAPTGDVKTGVSIVRKALIEP
                     LKQIAANAGVEGAVVVEHVSSLPFGQGYNAATGEYVDMIKAGIVDPAKVTRSALQNAA
                     SIAGMLLTTESLITDIPEKEPELPRGADMY"
     gene            complement(3690..>3865)
                     /locus_tag="PLW82_03930"
     CDS             complement(3690..>3865)
                     /locus_tag="PLW82_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOJ46456.1"
                     /translation="EVVAVGPGRKTDDGTVIPTEVKVGDKVLYAKYSGTQFKYDNVEY
                     LVVKESEILAILD"
BASE COUNT          970 a          971 c          933 g          991 t
ORIGIN      
        1 ccatctgcat ggtgtttgag agaaacgttg ctagtggcag cctgatcagc cagagggaaa
       61 gaaggctgac gcccatggcg ataaaggtat cgccagcgcc tctacagaga cctccggtgg
      121 taaacatcag agaatagacc acatagctca aggaaactat gcggaggtag gttacggtca
      181 tctcccgcac cgcaagatcc gtagtgaaga ttcttgccca gaaatcaggg aagaaccaaa
      241 tagccacggt gatgctagcg gcaattgcag tagttagaag gttggaataa cgccacacct
      301 cccgtactct ctcgggcttg tttgccccta agttctgagc aacaagggcc gatactgcaa
      361 gggccacact ttgagcgggc atgaaagcga actggtcgat gcggccgcca agaccgtaag
      421 cggcaacgac aatctcgccg ttacggttaa ccaagctaga gaggaaaagc atggcaattg
      481 aaaccagcgc ctgctgtatg ccagcgggag taccgatggt aatcagctgt ttaaggatga
      541 agccgtcggg cttgatcagc gacctgtccc aggttgcgat tttcgccttc ttaaatagat
      601 agatcagtcc aaaaaccaca gagatcgcct gggcaataac tgtcgcgtag gccactccgg
      661 cgattcccat tgcaggaatg ggaccaatgc caagaatgaa cagcgggtct agtatgatat
      721 tgatcactgc agcgatagcc aagaagtaga gaggtgttag agagtcgcca aagcccctca
      781 tgatcgccga aagcaggttg tagaagaacg caaaaatgat accgcccaaa atgatcacca
      841 ggtactcccc tgcaggtttg actaaactct cagggagact gatcaacctg aagatctgct
      901 tgtagaacag aagccctacc gtagtaacaa tgatcgatga gatcaccatt agaaaaatcg
      961 acgtgttgat gactctactg acgccttcct ggtctttcgc gccgtagtac tggctcacca
     1021 gaatcgttgt ggagatggta aagcctatag caagggctac cagaaagaag atgatgggaa
     1081 acgatgcgga aaccgctccc aaagcctctg tgcccaaaaa gcgtccaacc caaaacgtat
     1141 caactgtatt gtacagcgcc tgtaagaagt tacccaaaaa catcggccag gaaaaagcca
     1201 ctaggtgttt taaaatgctg cctgttgtaa aatcgcgagt tcgattattg ctcacagcta
     1261 ttacctcttt catctgtctt ttagttttat aatgctgcta aaatgagtcc atttgctatt
     1321 taaaacgaat ttaagtattc taaacgtagc agctttttcc tccatgaaac cagctgccat
     1381 tgtggtgttt tggagtactt cagcatcatt ctatgcgaca tgaaaaagcc tgtcaacgac
     1441 aaaccagagt aggacaggcg caaaataact ctaggaggga acgttagtgc agatcaatgt
     1501 tagatggaac ggcaagcgtc aatttattgg tgaatgcccc agcggccacg agttattgtt
     1561 agatagcagt cctgaagtcg gcggtgaaaa cgcaggtcca agaccgacgg aggctcttct
     1621 cacagccctt ggcggctgta ccggcattga tgtgattatg attctcgaaa agatgcagca
     1681 gaaggttact gcctaccaca tggagatcac cggagaaaga aacgccaccg aacctaagta
     1741 cttcaccgat atccatatta agtacgtgat caccggcgag aacctcagcg aagacagagt
     1801 aaaaagggcc attgacctct cttactccaa atactgcagc atcatccact cgctcaaggc
     1861 aaacatcact acatcctatg aaataattga ggctaagtaa ggagagcttg cagcctcctt
     1921 attcttaccc tgtttgttcc taaagaaaac cgagtctctg ccaaagagac gctcctttcc
     1981 tgtttcgggc atgaaaaaac ctgcagttca gtgaactgca ggtttatcta ttccttagta
     2041 catatcggct ccgcgaggaa gctctggttc tttctctgga atatcggtaa tgaggctctc
     2101 ggttgtgagc agcattcctg cgatagaagc tgcgttctgc agagcacttc ttgttacctt
     2161 agctgggtct acgataccag ctttgatcat atccacatac tcaccggttg cggcattgta
     2221 gccctgaccg aatggaaggg atgaaacatg ctctacaacc acagcgcctt caacgccggc
     2281 gttggctgcg atctgcttga gaggctcaat caaagctttg cgtacgatgg atactccagt
     2341 cttgacgtcg ccagtaggag cttcgttctc ggttttctcc agagctttag ctgcgtggat
     2401 caaggttact ccaccgcctg gaacgatacc ttcttcaact gcagctctgg tagcggacag
     2461 tgcgtcctca atgcggtgct tcttctcttt gagttcggtt tcggttgctg caccgactct
     2521 gataactgca acgccaccgg caagctttgc caagcgctcc tgcagcttct ctttatcgta
     2581 gtcggaagtg gactcttcga tctcagcctt aatctgagtg atgcggcctt tgatcgcttc
     2641 ctgagcacct ttaccgccaa cgattgtggt gttctctttg gttacgcgga ccttgtctgc
     2701 gcgaccgagc aaattgaggt tggcgttctc cagcttcatg ccggcctttt cagagatgac
     2761 ctgaccgcct gtgagaacag cgatgtcgcc gagcattgct tctctgcgat cgccaaatcc
     2821 aggagctttt actgccacaa cgttcagggt gccgcgcagt ttgttgacga taagagttgc
     2881 aagggcttcg ccctcaatat cttcagcaat gatcaggagc ggtttgccgg tctggagtac
     2941 tttttcaagt actggcagga tctcctggat aacgttgatc ttcttgtcgg tgataagaat
     3001 aaatggatct tcgagcacag cttccatgcg ctcggtatcg gttaccatgt aaggcgaaac
     3061 gtaaccgcgg tcgaactgca taccttcttc agactcgagg gtcgtaccga aggtctggga
     3121 ctcttctaca gtaatgacgc cttctttgcc aactttgtcc atggcttcgg cgatcagtct
     3181 gccgatctct tcgtcgcctg cagatactgc agctacgtga gcgatgcttt ctttatcctc
     3241 gataggctta gcgatgcttc tgatagcctc tactgcatct gcaacagcct tctcgatacc
     3301 ttttttcatc atcattgggt tggcaccagc tgcaacgttc ttcagacctt cgcgaatcat
     3361 ggcttgagcc ataacggtag cggtggttgt accgtcaccg gcgatgtcgt tggttttggt
     3421 tgcaacttct ttcaccagct gtgcacccat gttctcaaat ggatcttcca gctcgatttc
     3481 acgggcgatg gtcacgccgt cgttggtgat tgtcggcgag ccgaactttt tatctaaaac
     3541 cacattgcgg ccttttggtc cgagagtgac tttaacagcg tcagccaagg tgttaacacc
     3601 tttttctaac ttgcgtcttg cttcttctcc gaaaagcagt tgtttggcca tattgatccc
     3661 ctcctagttg ttttgttgtt gaaacatcac taatcaagaa ttgctagaat ttcggattct
     3721 ttcactacga gatactcgac gttgtcgtac ttgaactgag taccggagta cttcgcatag
     3781 aggactttgt cgcctacttt aacttcagta ggaatgacag ttccatcatc ggttttacgt
     3841 ccaggaccga ctgcaacaac ctcgc
//
LOCUS       DAOSDA010000126         3859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_14921_length_3859_cov_2.924027, whole genome shotgun sequence.
ACCESSION   DAOSDA010000126 DAOSDA010000000
VERSION     DAOSDA010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14921_length_3859_cov_2.924027"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            468..1175
                     /locus_tag="PLW82_03935"
     CDS             468..1175
                     /locus_tag="PLW82_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOJ46457.1"
                     /translation="MSYNIQVGWGKGLEAVCDLIKKYDPDVVAIQEVDRDRKRTGFVD
                     QFQTLKDLLGMHGGYACSYSGPASEGENAQYGHAIFAKYPVAVEKVHVLANRDYNLPG
                     EEAWVTEPRACLQSVINGIRFFSLHFSTVDDIQAMQAAQLAEIIKANSDLPVISLGDF
                     NNDYYSEALKPLTFLKNGLEEIEPALSYPNGSRAVSAIDHILLSDHFEVLGAQVVVEN
                     EGISDHNPVICDIRLKG"
     gene            1187..2197
                     /gene="ltaE"
                     /locus_tag="PLW82_03940"
     CDS             1187..2197
                     /gene="ltaE"
                     /locus_tag="PLW82_03940"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010199427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOJ46458.1"
                     /translation="MIDLRSDTVTKPTAKMREVIFNAEVGDDIYGEDPSINELEAYSA
                     ELFGKEAALFVTSGTQGNTLAILSQTQPGNEVIVESNAHIFWYEGGAACAIGGVQLHP
                     VPGTRGVMTGADVEARIRPKGNVHFPQTALVCLENTHNRAGGTYWSLDQVKEVKDVAD
                     KHGLKVHMDGARIFNASVATGTPVKEYAKYVDTVQFCLSKGLGAPVGSVLVGPKETIQ
                     KARFWRKRLGGAMRQAGIIASAGLYALQHHIDRLEDDHKLAKKLALGLKNLGLDVDPD
                     SVKTNMVMLRFADCDRLLAKLKEKGILAGLSVPGVIRFVTHLDITEEDIDTTIKTIAE
                     LI"
     assembly_gap    2383..2392
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2393..3084)
                     /gene="ligD"
                     /locus_tag="PLW82_03945"
     CDS             complement(<2393..3084)
                     /gene="ligD"
                     /locus_tag="PLW82_03945"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOJ46459.1"
                     /translation="MSSLYNLTNLDKELWPGITKKDLDTYFLYVAPAMLEYVKDRPLS
                     LVRAPDGVGGETFFQKNLPEHAPNQLRSWEYVRGKRSTRYLVLKSVEDFVYLGNQATI
                     EIHSMLSTVQEVERPVEIAIDLDPREGTEFLLVKQGALLVKELLEQLGLRSYPKFTGK
                     KGIHVMIPIEPEYTYGEIAPFLKALRELVVATYPELFTGHLRKEKRDAPIFLDIGQNG
                     LEKTVIVPYSPRT"
     gene            complement(3050..>3859)
                     /locus_tag="PLW82_03950"
     CDS             complement(3050..>3859)
                     /locus_tag="PLW82_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46460.1"
                     /translation="WKRWSKMYLPMLATEAEAAFDSPEYIFEPKWDGYRALYYTKLGE
                     IRSRRGKSLTETFRHLTKTSIDEAVIDGELVSFASGRPDFASLRKNPQTAVLMAFDLL
                     SVGGRDVTKLPLESRKEILKELIQGQHLIGFSPDYSDGIKLFAALKDAGWEGIVAKRK
                     GSVYRPGERSSDWLKIVAYKEIDAVVLEVIKERGTALLGLFRGGHLEPVGHVPLSRYA
                     DLEQKGPQVVTVRYREYTGKAFRHARFVRTREDKSPWECTYEQLVQPHKPR"
BASE COUNT          989 a          971 c          893 g          996 t
ORIGIN      
        1 accatttctt tggctacggt taaatgtagc tgattctttt ttagcggagc aagctactat
       61 gtgcatctcc agtaatgata acagcaaata ccggctattt ccaaaagaag ttgcagaaga
      121 aaccaaactg attcctgatc agtaacctag gatcgacgca tcttcttttt tatagtctac
      181 actgaatcaa ctaagacgtc tcttgaaaaa cctgcataag tcaggtgttt cctacttatg
      241 gtttgcacaa gttcttaaga ggtgaaatca aacctttgtc aaataactac aaagaaccac
      301 cttatggtgc ttagaggcac cgactaaata gacaacatca actccttttc ccccaaagtc
      361 gtccgattcc cggacgactt ttcttggttt ttgagaggat ttttcaggtc gatcgccaaa
      421 gcatcagtgt atattttgat tcattggagg tagcagattt gagaataatg agttataaca
      481 tccaagtcgg ctggggcaaa ggtcttgaag ctgtctgcga tcttataaaa aaatacgatc
      541 ctgacgtagt agcaatacaa gaggtcgata gagaccgtaa gcgcaccggt ttcgttgacc
      601 aatttcaaac ccttaaggat cttctgggca tgcacggcgg ctacgcatgt tcttacagcg
      661 gtccagcatc tgaaggagaa aatgcccagt acggtcacgc aatctttgcc aagtatcctg
      721 ttgccgttga aaaagtacac gttctggcaa acagagacta taaccttcca ggcgaagaag
      781 cttgggtcac cgaacctcgc gcttgcttgc agtcggtcat aaacggcatc aggttctttt
      841 cgctgcattt ttcaacggtc gacgatatcc aagcaatgca agcagctcag ctggcagaaa
      901 tcatcaaagc aaacagcgac ctgccggtta tttctcttgg cgacttcaat aacgactact
      961 attcagaggc actaaagccc ctcactttcc tcaagaacgg tctggaggaa atcgagcctg
     1021 ctctcagcta tccaaatggt agtagagctg taagcgctat cgatcacatc ctcctcagtg
     1081 atcatttcga ggttttaggc gctcaagtag ttgtagagaa cgaaggcatc tccgaccaca
     1141 acccggttat ttgcgatatt agactcaaag gataggggat gtgaacgtga tagatctgag
     1201 aagcgacacc gtaaccaaac caactgcgaa gatgcgtgaa gtgatcttca atgcagaagt
     1261 tggtgacgac atctatggcg aagatccgtc tatcaatgaa ctcgaagcct acagcgcaga
     1321 actctttggt aaagaagctg ccctgtttgt caccagtgga actcaaggca atactctggc
     1381 aattctctct caaacgcagc cggggaacga agtaatcgtg gaaagcaatg cccacatttt
     1441 ttggtacgaa ggcggcgctg cctgcgcaat tggcggggtg cagttgcatc ctgtaccagg
     1501 cacgcgcggc gttatgactg gagcggacgt tgaagctaga atccggccta agggcaacgt
     1561 tcatttccca cagacggccc tcgtctgcct tgaaaacacc cacaatagag ctggcggtac
     1621 ctactggtct ttggaccagg taaaagaggt taaggatgtt gcagacaagc atggtctgaa
     1681 agtgcatatg gatggtgcca ggatcttcaa cgcctcggta gcaactggga cacccgtcaa
     1741 agagtacgca aaatatgtgg acacggtgca gttttgcctc tccaaaggcc tcggtgcccc
     1801 tgttggttcg gttctcgttg gcccaaaaga aaccatacaa aaagctcgct tttggagaaa
     1861 gaggcttggc ggagccatgc gtcaggcagg tatcatagct tctgcaggtt tgtatgcgct
     1921 tcagcaccac attgataggt tggaagatga ccacaaacta gcgaagaagc tggccttggg
     1981 gctgaaaaac ttagggctcg acgtggatcc agacagcgtt aagaccaaca tggtgatgct
     2041 tcgtttcgca gactgcgatc gtctcttggc aaagcttaag gaaaaaggta ttcttgcagg
     2101 gctttcggtt cccggagtca tccgctttgt cacccatttg gacataactg aggaagatat
     2161 cgatactacc atcaagacca tcgccgaact gatatagaga aggtgccttc acgggcacct
     2221 ttttttgctt cacagctacc tgacagcctc taccggaagg ccttgcatga tctgaggtgc
     2281 attgaccaaa ggtggccaaa aaccttctct aacgttaaag acaggtcttt caaccatcct
     2341 ctctaagtcc tgccaggtag atatcactgc gactttagcc tcnnnnnnnn nngttctcgg
     2401 gctgtaaggg acaattaccg tcttttccaa accattttgc cctatgtcca gaaagattgg
     2461 ggcatctctt ttttccttcc ggagatgccc ggtaaagagc tctgggtaag tagctacaac
     2521 caattctctt aacgccttta agaatggggc aatctcccca taggtatact caggctcgat
     2581 gggaatcatc acgtgaatac cctttttccc tgtaaacttc ggataacttc tcagtcccag
     2641 ttgctctaga agttctttaa caagaagagc tccttgctta accaacaaga actcagttcc
     2701 ttccctggga tccaagtcga tagctatctc taccggcctc tccacctctt gtacggtgct
     2761 caacatagag tggatctcta tggttgcctg gttcccaaga tagacaaagt cttctacaga
     2821 ctttaacacc agatagcggg tggatctttt ccctcgtaca tactcccaag acctgagctg
     2881 gttaggggca tgctccggca gattcttttg gaaaaaagtc tctcctccga cgccatcagg
     2941 tgctctcacc agcgaaaggg gccggtcttt cacgtactcc agcatcgctg gtgcgacgta
     3001 aaggaagtag gtgtctagat ccttcttggt gatccccggc cagagttcct tatcgaggtt
     3061 tgtgaggttg tacaagctgc tcataagtac actcccaagg cgatttatct tctcgcgtgc
     3121 gaacaaaccg ggcgtggcga aaagccttcc cggtgtactc tcggtacctc actgttacca
     3181 cttgaggtcc tttttgttcc aagtcggcat atcggctcaa agggacatgc ccaaccggtt
     3241 cgaggtgccc acctctgaag agccctaaaa gggcagttcc tcgctctttt attacctcca
     3301 gcacaacagc atctatctct ttataggcaa cgatcttcaa ccagtcgcta gagcgttctc
     3361 caggccgata tacagagcct ttgcgttttg caacgatccc ctcccagccg gcgtccttta
     3421 gcgctgcaaa gagctttatc ccatcagagt agtccggtga aaaaccgatt aggtgctgtc
     3481 cttgaatcag ctctttcaag atctctttgc gtgactcaag gggcagtttt gttacatccc
     3541 tgccgcctac cgagaggagg tcaaaggcca tgagcactgc tgtctgtggg tttttcctta
     3601 gagaggcaaa atcaggccta ccgcttgcaa aactcaccag ttctccgtct ataaccgctt
     3661 catcgatact ggttttcgtt agatggcgga aagtctcagt gagactcttt cccctccgac
     3721 ttctgatctc accaagtttt gtatagtaaa gggctctgta cccatcccat ttcggttcaa
     3781 agatgtactc aggcgaatca aaggccgctt ctgcttcagt ggcgagcatt ggtaggtaca
     3841 ttttgctcca ccttttcca
//
LOCUS       DAOSDA010000127         3857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_14928_length_3857_cov_3.069437, whole genome shotgun sequence.
ACCESSION   DAOSDA010000127 DAOSDA010000000
VERSION     DAOSDA010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14928_length_3857_cov_3.069437"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PLW82_03955"
     CDS             complement(<1..1009)
                     /locus_tag="PLW82_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ46461.1"
                     /translation="MHLIAFAIRTLPAEVKNNWQRSLIIILCLAVGIASVAAVKIYTD
                     SALSYFTGNVKEFVGADITVQLPGELTEDQENFLNNQTLGIITYVENYNQVAFNPFNR
                     QKNMPVQVTVIDPEVYPLYGSMELADGSSAQALLDEPQAALVSASLFEQLELKMDNLV
                     LVGNVPYIVRGILKETSVPDGFMGRIYTKTPPQEGVGTLGSRFAYIKLDEGKDPEIAK
                     KEILEFFEPGGVSTYVEVSSQISRSAKVLGNVALIASLASLLLGGLGVSNVAQVISRQ
                     KLKQSALMKCVGGRTSQITLIIVIQIAFIGLIGVLCGFVLGWALTGVLPGLLKDLVSY
                     NI"
     gene            complement(1021..1680)
                     /locus_tag="PLW82_03960"
     CDS             complement(1021..1680)
                     /locus_tag="PLW82_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006211152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46462.1"
                     /translation="MEILRTIELGKTYRDGEKETEVLRGINLEIDEGEFVAIAGPSGS
                     GKSTLLGLLAGLDRPTTGQVFIGETEITTLPESRLAKIRGRMLGFVFQSFNLIPTLTA
                     LENIELPMILNKKPDPQRARELLDLVGLSHRSRHYPSQLSGGEQQRVAIARALVCDPP
                     IILADEPTGNLDSKNSQTVIDLLLSLNSEKQKTIVLVTHDMDFAAKAQRIVHINDGRI
                     S"
     gene            1950..2171
                     /locus_tag="PLW82_03965"
     CDS             1950..2171
                     /locus_tag="PLW82_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46463.1"
                     /translation="MKIIDMKKWQRQRRRNARGQRFSRFYSTRVFVNMGSVILIGYVL
                     SFLVPVWLSVIIAMVIYTYGREFWAKFRP"
     gene            2197..3306
                     /locus_tag="PLW82_03970"
     CDS             2197..3306
                     /locus_tag="PLW82_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46464.1"
                     /translation="MKDWQDEMKQKFEVLLDVFNQYFLTQVVESGETGFDIKDEMLGS
                     MASSCTTLEAALSLFTGTLFADGSHDNTLSIAQGLVESAGLRQHADGALGQPFYILPG
                     SGETKDIAEIGAAADALYFLYRYGKAAAAKDILLKAAGFLEKMSHPEVKGVYYKRPDA
                     THHDVLNGDAYAGAALMRAYQITGEKKYHDWASEVAAHLIRRFGAHQEGWWPYAEYFD
                     GRVDVGNSLGYQATVIAFGHDILDGMEDQGLKSEFAGVLDQAMLKVIECIEADLSLRE
                     VESVWWASPWNKSAEVFLALVKQRHQEKARKYAAVQLNEISQNVLGQEGISYFIPPKR
                     DEADPNRTPVTTTFRKVATMAGIISYAYMDLWERG"
     gene            3446..>3857
                     /locus_tag="PLW82_03975"
     CDS             3446..>3857
                     /locus_tag="PLW82_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46465.1"
                     /translation="MTVSGKNKAMWSLIILLLISAFASAFDVAIIGVKPYTTSQWTDA
                     EKISGTLYKTLGDVSSEVNVLTSAQVTALIGDRRVFLESYSGGMPQVPKVDAVVIGEI
                     VPQGNSYRTTLRLYNLLSKESIQYSLVGKPGDLDK"
BASE COUNT          980 a          917 c          996 g          964 t
ORIGIN      
        1 gtatattgta ggacaccagg tctttgagaa gaccaggcag gactccggtc agcgcccaac
       61 ccaaaacaaa cccgcagaga acgccgatca atccgatgaa cgctatttgg ataacgataa
      121 tcagcgtaat ctgtgaggtt ctccccccga cgcacttcat gagtgcactc tgcttcaact
      181 tctgcctgga gataacctga gcgacgttgc taacgccaag accgcccaaa aggagcgatg
      241 ccagcgaagc gataagcgcc acgtttccaa ggactttggc ggagcggctg atctgcgacg
      301 agacttccac gtaggtggaa acgccacctg gctcaaagaa ctccaggatt tcctttttcg
      361 caatttcggg atctttacct tcgtcaagct tgatgtatgc aaatctgctg ccaagagtcc
      421 ccaccccttc ctgcggtggg gtcttggtgt aaatgcgacc cataaatcca tcgggcacag
      481 aagtttcctt gaggattccc cgtacgatgt agggtacatt gcccacaagt acaaggttat
      541 ccattttcag ctccagctgc tcgaacagag aagcagaaac cagcgcagct tggggttcat
      601 ctagaagtgc ctgtgcactg ctgccgtctg ccagttccat ggaaccgtag agcgggtaga
      661 cctcagggtc gatcacagtg acctggaccg gcatgttttt ctgcctgtta aacgggttga
      721 aagccacctg gttgtagttt tcaacgtagg tgataattcc cagagtctgg ttgttcagga
      781 agttctcctg atcttctgtg agctcacctg gcagctgtac ggtgatgtct gcaccgacaa
      841 actccttcac gttgccggtg aagtacgata gtgccgaatc ggtatatatc tttaccgcgg
      901 cgaccgatgc aattcccaca gccaaacaga ggataataat cagtgatcgc tgccaattgt
      961 tcttgacttc tgccggcaga gttctaatgg caaaagcgat aaggtgcatc gatatacccc
     1021 ctatgagatc ctaccgtcat tgatgtgcac gattctctgg gctttggcag caaaatccat
     1081 atcgtgagtt accaaaacga tggttttttg cttctcggag ttcaggctga gaagcagatc
     1141 gatcaccgtt tggctgttct tgctgtcgag gttgccggtg ggttcgtctg ccagaataat
     1201 gggcggatcg cacaccaggg ctctggcgat agcaaccctc tgctgctcac cgcctgaaag
     1261 ctggctgggg tagtgcctac tcctgtggga gagtcccaca agatccagca gctctcttgc
     1321 tctttgcgga tctggttttt tgttcaagat catgggcagt tcgatgttct ccagtgcagt
     1381 cagcgttggt atgaggttga acgattggaa gacaaatccc agcattctgc cgcggatctt
     1441 ggctagacgg ctttctggca gggtggtgat ctccgtctcg ccaatgaaca cctggcctgt
     1501 agttggtctg tcgagccctg ctaaaagacc cagcagcgtg cttttgccgg aaccggaagg
     1561 gcctgcaatg gcaacaaact ccccttcgtc gatttcaaga tttatcccac gaagaacttc
     1621 cgtttctttc tcaccatctc ggtaggtttt accaagttcg attgtgcgca gtatttccat
     1681 ggttagactc aagaatgcct tgagttttta cacctccaac ggatcatttg ataggacaac
     1741 ctgcatgtac cgacagtatg agacctcctg gcttttagtt acataatacc gtacattagg
     1801 gacaaaagaa agtcaaagcc cagctcggtg aggtccagat accaaaggtt tttttcggct
     1861 agattcctga cgactacagc gaaaaatgat agactaaagg acagaagaaa gcggtttata
     1921 cttctgttta taggagttga gttggtataa tgaagatcat cgatatgaag aagtggcaga
     1981 gacaacgtag aagaaacgca agaggacagc ggttttcccg cttctattca acgcgggtct
     2041 ttgtgaacat gggctctgtt atcttaatag gttatgtact cagcttctta gttcctgttt
     2101 ggctcagtgt gatcatagcc atggtgatct atacctacgg cagagagttt tgggccaaat
     2161 tccgtccata gcaaagtaaa ctcgaggtga tcatcgatga aagattggca agacgaaatg
     2221 aagcagaagt tcgaagtgct ccttgatgtg tttaaccagt actttctcac tcaggtagtg
     2281 gagtccggtg agacaggttt tgacatcaag gacgaaatgc tcggttcgat ggcaagcagc
     2341 tgcaccacat tagaagctgc gctttctttg tttaccggaa ccctttttgc agacggttct
     2401 catgataata cattgtcaat tgctcagggg ctggtagaaa gcgctgggct aaggcagcac
     2461 gccgatggtg ctttggggca gccgttctac atccttccag gtagtggtga gaccaaagac
     2521 atcgccgaga ttggggcagc agcagacgct ctgtactttc tatatcgcta cgggaaagct
     2581 gccgcagcta aagacattct tcttaaagct gctggattcc ttgaaaaaat gagccatcct
     2641 gaagttaaag gcgtatatta caaaagacca gatgccaccc accatgacgt cttaaacgga
     2701 gacgcttatg ctggggctgc actgatgcga gcttaccaaa taaccggaga gaagaagtac
     2761 cacgactggg cgtcggaggt ggccgcacat ctgatccggc gctttggcgc tcaccaggaa
     2821 ggctggtggc cttacgcaga gtacttcgac ggccgagttg atgtaggcaa tagtctcggc
     2881 taccaggcaa cagtcatcgc tttcggtcac gatattttag acggcatgga agaccaaggg
     2941 ctcaagtccg agtttgccgg ggttctagac caggccatgc tcaaagtcat cgagtgcatc
     3001 gaggctgatc tttccttgcg agaagtggag tctgtatggt gggcttcgcc gtggaataag
     3061 agtgcggaag tctttcttgc actagtgaag caaaggcatc aagaaaaagc caggaagtac
     3121 gcagctgtgc agcttaacga aatctcacag aacgtcttgg gacaggaagg catcagctac
     3181 ttcatcccgc cgaaaagaga tgaagcagac cccaacagga cgccagttac taccactttt
     3241 aggaaagttg caaccatggc tggaattatt agttacgctt acatggatct ttgggagcgc
     3301 ggttgatgtt tccatcgtct aatatttgcc gggggcaaat ctggtaacgt ttacctgcaa
     3361 caccagtcac agtttatgga gggaaagggc aggtaaacct taaatattct tcgtgaagga
     3421 ttttcccaga aaggggatga ttgacatgac agtctccgga aaaaacaaag ctatgtggag
     3481 tttaattata ctcctattaa tctctgcttt tgccagtgca ttcgacgtgg caattatcgg
     3541 ggtcaagccg tacaccactt ctcagtggac cgatgctgaa aaaatcagcg gaactctgta
     3601 taaaacgctc ggagacgtat ccagtgaagt aaacgttctt acaagcgctc aggttacggc
     3661 gctgatcggc gacaggaggg tattcctcga atcttactca ggaggtatgc cgcaggttcc
     3721 taaagttgat gctgtggtta tcggggagat cgttccgcaa ggcaactcct accggacgac
     3781 cctcaggctt tacaacttgt taagcaaaga gtcaatccaa tactccttgg ttgggaaacc
     3841 tggcgatttg gacaaaa
//
LOCUS       DAOSDA010000128         3849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_14975_length_3849_cov_2.459673, whole genome shotgun sequence.
ACCESSION   DAOSDA010000128 DAOSDA010000000
VERSION     DAOSDA010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14975_length_3849_cov_2.459673"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..626)
                     /locus_tag="PLW82_03980"
     CDS             complement(96..626)
                     /locus_tag="PLW82_03980"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOJ46466.1"
                     /translation="MAKYFPKIIGDAVYLSPMSLDDLEQYTRWMNDLEMTKYLGAAAT
                     TISLEKEREALEMLAKGEHNFAIVLKGEDRLLGNISLMKVNHLHQTAEVGLFIGNKED
                     RGKGYGSEALRLIVDFGFKYLNLKNIMLKVNAENKAAIAAYKKCGFNEFGRRTRSVYV
                     DGKWCDDIYMEVLREW"
     gene            complement(957..1883)
                     /locus_tag="PLW82_03985"
     CDS             complement(957..1883)
                     /locus_tag="PLW82_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098500.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOJ46467.1"
                     /translation="MRWFDKRYHTLDHEMKTTFGRKVAKLSLDGGFTCPNRDGTLGSR
                     GCIFCSEVGSGEFAGSRNLGIREQIEEQKRLLSHKWPGAKYIAYFQNFTSTYGSIEKL
                     ERLYREALSCPDVVGLAIATRPDCLPAETVELLADLSKATYLWVELGLQTIHPKSAVF
                     IRRGYDLECFEAAVENLKEKRIRTAVHLIFGLPGESREEMLESADYVAGIGVWGVKIH
                     LLHVLMDTDLHRYYLKNPFPLMSREEYTGVVCDAIEILPPDMVVHRLTGDGKKDRLVG
                     PWWSLDKLRVLSGIDQELKKRDSFQGIKYREI"
     gene            complement(2232..2666)
                     /locus_tag="PLW82_03990"
     CDS             complement(2232..2666)
                     /locus_tag="PLW82_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOJ46468.1"
                     /translation="MSVKPYLIFNGNCREAMDFYREVFGADEPVVMTYGDLPKGNDDF
                     SLPPEAKSLVLHAEMTIAGDTIMFSDQFPGMPYNVGNHMSLAVISDDMDEIKSWFAKL
                     KVGGQVEMELGETHWSKCYGSVFDRFGVSWQLNYSEPETVQP"
     gene            2921..3520
                     /locus_tag="PLW82_03995"
     CDS             2921..3520
                     /locus_tag="PLW82_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ46469.1"
                     /translation="MSLTAGGSISNLHLDSQAKLIEGLKNGDENAVKQLVFLYSDRLL
                     QVATLILKDQYLAEDVVQESLLVAINKIHQLRGDDLSPWLYRILVNRCRKEQRKKPWS
                     VVEYLPKNELELVPATGVDVERDVLEKDTDREVMYAIAQLPARYREVVTLCYLEEFSL
                     ETIAKVTQEPLGTIKSRLHRARKRLQKILIEGGFFDGFE"
     gene            3507..3830
                     /locus_tag="PLW82_04000"
     CDS             3507..3830
                     /locus_tag="PLW82_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46470.1"
                     /translation="MDLNDRKLKQKLKSEIGSIKFSSYQKNQLAEALIAGQKKENLPK
                     RAFHLLSEFWHGTTEIPLYLGAIGLLVLILGVRNTVDIFLIDSESAAFLLQQTVEIIE
                     RSGLW"
BASE COUNT          919 a          996 c          882 g         1052 t
ORIGIN      
        1 acgctttcat gcccggaggt aggttaacct ctcaggcaaa tactgcagaa tgcagtgtat
       61 ggagttttcc aagacaacga gtctctcgcc ttcctttacc actctcgcag aacttccata
      121 taaatatcgt cgcaccactt gccgtctaca taaacgctcc ttgttctcct gccaaactca
      181 ttgaagccgc actttttgta tgcggcgatt gcagctttgt tttccgcatt taccttgagc
      241 atgatgtttt tcaggttcag gtacttaaaa ccgaagtcta caataagccg tagggcttcg
      301 gagccgtacc ccttacccct gtcttccttg tttccgataa atagccctac ttcagcggtt
      361 tggtgcagat ggtttacctt catcagcgag atgttgccga gcaggcggtc ttctcccttc
      421 agaacaatgg caaagttgtg ctcgcctttt gcaagcatct ccagggcttc ccgctctttt
      481 tccaagctga tggttgttgc agcagcacca aggtactttg tcatctccag gtcgttcatc
      541 caccgggtgt actgttccag atcgtccaga gacatgggag ataggtaaac cgcatctcca
      601 atgatcttcg ggaagtactt tgccatactt ataccacact ttcatcttaa ataaaaggct
      661 ttccagaaag tgcaacgtgc gtctttcccg cgttacagcc caatacgaaa aaaccggtcc
      721 caaaatcgca ctgaatctgc gaatatttac cttaacagct cctctgcaat agttctgtag
      781 ttgttcgcca agaaatgcgt tgttccttgc gatttctgcc gctttgtaaa acttttttta
      841 tggtctactt aaaaagcttc gctgccaaaa aagccgatgc atgagcatcg gctgaagcgc
      901 tttttaaggg ttccccgcag catctcaagt cctcggtttt ttgagagtga cggtttttat
      961 atttctctgt actttatgcc ctggaaactg tcccgctttt ttaactcctg atcgatccct
     1021 gaaagcaccc tgagcttatc gaggctccac caaggcccta ctagcctgtc ctttttgccg
     1081 tctccggtga ggcggtgcac aaccatatca ggcgggagga tctcaatggc gtcgcaaaca
     1141 acaccggtat actcttccct ggacataagc gggaaggggt ttttcaagta atatctgtgg
     1201 aggtcggtgt ccatcagtac gtggagaagg tggatcttta cgccccaaac cccgatccct
     1261 gccacataat ctgcgctttc tagcatctct tcccgagact cgccgggaag tccgaagatg
     1321 aggtgaacag cagtcctgat tctcttttcc ttaaggttct ctacagcagc ctcgaagcat
     1381 tccaggtcgt atccccttct aatgaagaca gcgcttttag ggtgaatggt ctgcagcccc
     1441 agctccaccc agagataggt tgctttggac agatctgcaa ggagttccac cgtttctgca
     1501 ggtaggcagt cgggacgggt ggcgatggca agccctacaa cgtctgggca ggaaagagct
     1561 tctcggtaga gtcgctccag cttctcaata gatccgtagg tgctggtgaa gttctggaag
     1621 taggcgatgt attttgcgcc tggccacttg tgtgaaagga gcctcttctg ctcttcgatc
     1681 tgttccctga tgccgagatt tcggctaccg gcaaactcgc cgctgcctac ctcgctgcag
     1741 aagatgcagc ctctcgatcc aagagtccca tctctgttgg gacaggtgaa gccgccgtcg
     1801 agggagagct ttgccacctt cctgccgaag gtggtcttca tttcgtggtc tagtgtgtga
     1861 taccttttgt caaaccatct catttttgcc accacgatta agtaatatga acgtctcttc
     1921 cgcttcctct tatgcacacc ctgcaaactg ccagcttcat ttgcctctta cattatacca
     1981 agagtctttt ggttctactt atgcttaaaa cttgttttgc tgtttttttg ttacgttcaa
     2041 cgtctttaat acatttgtaa cactcttaat actgactgtg agtaccccat ataccgggcg
     2101 gatcacatat agtagatagc gatgcatact agaatgctcc ctctacatcc attgcgcgag
     2161 agagcatttt tgtcaatgaa ctgtccgggt ttaatgcctc gatgaagcac ctacatccaa
     2221 ctcaaaactt cttagggctg cactgtttct ggttctgagt agttcagctg ccaagagaca
     2281 ccaaacctat cgaacacaga accgtagcac ttgctccagt gggtttctcc cagttccatc
     2341 tccacctgac cgccgacttt gagctttgca aaccaggatt tgatctcgtc catgtcatcg
     2401 ctgatcacag caaggctcat gtggtttccc acgttgtagg gcatgccagg gaactgatcg
     2461 gaaaacatga tagtgtcgcc ggctatggtc atctctgcgt gcaggacgag actctttgct
     2521 tcagggggaa gagaaaagtc gtcattgccc tttggaaggt ctccataggt cattactacc
     2581 ggttcgtcgg caccgaaaac ttccctgtaa aagtccatcg cttccctgca gtttccgtta
     2641 aaaatcaaat acggctttac ggacatgtct atcacctctg atgtagattt acccaaccat
     2701 cggtgtttga atctacttcc gtcacttggt ttgcaagttt ttcgcgttct tctattgtct
     2761 tacaacgcta caagccgttt ttggggttgc ttttggaaag agggtatttg aactctttga
     2821 tcaatactat aagcagcaga ccgctctgga aattttttct gtcaaaaatg caaccttttg
     2881 aaaacgaagc ggctcgttta aagtgaagga ggagattttt gtgtccctta ccgcgggagg
     2941 gtctatctcc aatctacact tggactcaca agcaaaactg atcgaaggac taaaaaacgg
     3001 ggatgaaaac gccgtaaagc agctggtctt tctctactcg gacagactgc tgcaggttgc
     3061 aaccctcatc ctcaaggacc agtacctggc agaagacgtg gtgcaggaat ctcttcttgt
     3121 agccatcaac aaaatccatc agcttcgggg cgacgatctt tctccctggc tgtaccggat
     3181 tttggtgaac cgctgccgca aggagcagcg gaaaaagccc tggtcggtgg tggaatacct
     3241 gccaaagaac gagcttgagc tggttccggc cacgggggtt gatgtggaaa gggatgtgtt
     3301 ggaaaaggat acagaccgcg aagtcatgta cgccatcgca cagctgcctg caaggtatcg
     3361 ggaagtagtt acactctgct acctcgagga gttctccctc gagaccatag ccaaagttac
     3421 ccaggaacct ctcggcacca tcaaaagcag actgcaccgg gcgcgcaaac gactccaaaa
     3481 gatcctgatt gaaggagggt tttttgatgg atttgaatga tcgcaagctg aagcaaaagc
     3541 taaaatcgga aatcggtagc attaagttct cctcttacca aaaaaaccag cttgcagaag
     3601 ccctcatcgc cgggcagaag aaagagaacc ttcccaaacg cgccttccac ttgctctcgg
     3661 agttttggca cggcaccacc gaaatacctc tttaccttgg agctatcggt cttttggtcc
     3721 tgattctcgg tgtaagaaac accgtcgata ttttcctcat tgacagtgag tctgcagcat
     3781 tcctcctcca acaaacagtt gaaattatcg agaggagcgg tctttggtga tgcggacgat
     3841 aaagatcaa
//
LOCUS       DAOSDA010000129         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15231_length_3803_cov_3.098986, whole genome shotgun sequence.
ACCESSION   DAOSDA010000129 DAOSDA010000000
VERSION     DAOSDA010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15231_length_3803_cov_3.098986"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..2613)
                     /locus_tag="PLW82_04005"
     CDS             complement(58..2613)
                     /locus_tag="PLW82_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HOJ46471.1"
                     /translation="MQAKRLAVFILVLLLVQIVHAKVYDIFPTPQTLILGTESFPLES
                     FSLMWKVDADLKDKFQFSIVESEDAPRKISVTIGETVELSHYGNEAYLIEVTDEGGRI
                     VAISERALYYALITFKQLIKDAVELPEVTIIDAPDYQIRGVIEGFYHDPWSHAARVDM
                     VRFLGESKMNTYVYAPKDDPYHREKWREPYPDEKLAQLKELVQAAKASYVDFVFAISP
                     GLSIEFSSESDWQKLLAKTDAMIEIGITHFALLLDDIDPNLRSARDRAKYGNDYALAQ
                     VDLCNRYQDYLAAKIPGHRLIVVPTEYYQEGTSPYRRTYNTKLSPDIIVYSTGYGIVA
                     QTITPKEAGEIYEIWGHDIVYWDNYPVNDYNRTKLFMAPIAGRGPTLPGTLGFTFNPM
                     NEAELSKLPLLTCADYTWNSASYVPEHSWERAVAILGGEHQDTCRRFAEQNLVFFPDG
                     PQQEYPTLTNLAQEFLTAHKAYAEARKSSADAPIVDELKDDTLAKLKALQDEFRALEG
                     LRGEFEEYFPLAAKEAAPYLKRLENWGKVGAYYTDTLLKILEAGPLSSSTVTPEMLST
                     YSTALETYLKGLALNRFDVLSSSQIGSSTVVLPILDSLGRVFASNFTAETIVASTNMP
                     VWQNYVPANAVDSDTGTFFWSSRGQRPGDFFMVDLGSVQTLSGISLLMGNSNLDYFHK
                     CIVEISADGTLWEAVATLEGTPDHIIQFFEPKTARYLKVTGLVEKEYWIIIRQFEPIY
                     TEKKTAFEYDRTMVDVFNKIAVDPLFIIESEITVSLEVPVPIETAGIIQDPSLPTTWT
                     LSYSLDGETFIEGAVLDSLVQVLPLPPDPIKALRFTPHEEGTYLRSIFVVPKN"
     gene            2936..>3803
                     /locus_tag="PLW82_04010"
     CDS             2936..>3803
                     /locus_tag="PLW82_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOJ46472.1"
                     /translation="MANSKVTKKPSASSGDNFFERVFKLKEHNTTIGIEVMAGLTTFM
                     TMAYIIAVNPGMLAETQQVSFDAALIATVLSAAFATFLMAFLANYPFALAPGMGLNAY
                     FVYTVIMGMGYTYAQALTAVLISGIVFVILTVTGLRQVIVKAIPNNLKAGIGAGIGLF
                     IAFLGLKNAGIMVSDSATLLGLGDMTSAPVILAIVGLIVTAVLMYKNVKGSILIGMAV
                     TTVLAWITKVAPGPAGIVAWPNLGAWSSEALFKLSGFKTLGILGVVEVVFAFFFVDMF
                     DTIGTLVGVAKRS"
BASE COUNT          924 a          843 c          989 g         1047 t
ORIGIN      
        1 ccgttctgtg agaggaaaac cgctaggctg atcacctagc ggttacctac tcgatttcta
       61 gttcttagga acaacaaaga tcgatctgag ataggtgcct tcctcgtgag gggtgaacct
      121 taaagccttt atcgggtcag gaggtagggg taagacctga actaaactat cgagaaccgc
      181 cccttcgata aaggtttcac cgtcgaggga gtaggacagg gtccaagttg tgggtagcga
      241 tgggtcttgg atgattcctg ccgtctctat tggtacgggc acttccaaac taacggtgat
      301 ttcactctca ataatgaaca acgggtctac ggcgatcttg ttgaagacgt cgaccatggt
      361 gcgatcatat tcaaaagctg tctttttctc ggtatagatc ggctcaaact ggcggatgat
      421 gatccagtac tccttctcaa caagcccggt gaccttcagg tatcgtgcgg ttttaggttc
      481 gaagaattgg atgatgtggt ctggagtacc ttcgagggtt gcaaccgctt cccagagagt
      541 accgtcagcg gagatctcta ctatacactt gtggaagtag tcgaggttgc tgttccccat
      601 caaaagagaa ataccactta gagtttgaac gctgccgaga tctaccatga agaagtcgcc
      661 tggtctctga cctcttgaac tccagaagaa ggttccggta tctgagtcta cggcatttgc
      721 cggcacgtaa ttctgccaga ctggcatgtt tgtggaagca actattgtct cggcagtgaa
      781 gttagatgca aagactcttc ctagactgtc gagaatgggc aggactactg tggagctgcc
      841 gatctgagat gaagagagga catcgaagcg atttagagca agccccttca gatacgtctc
      901 taaagctgtg ctgtacgttg agagcatttc cggtgttacg gtactgctag agagcggccc
      961 agcttcgagg attttaagaa gggtgtctgt gtagtacgca ccaaccttcc cccagttttc
     1021 caaccgcttc aggtatggtg cagcctcttt tgcagctaga gggaaatact cctcaaactc
     1081 tccccttaag ccctccaaag cgcggaattc gtcctggagc gctttgagct tagccagtgt
     1141 gtcatctttc aactcatcga cgatgggagc gtctgcggag cttttgcgag cctctgcgta
     1201 ggctttgtgt gctgtgagga actcctgtgc taggttggtc aaagtcgggt actcttgttg
     1261 tggtccatct gggaaaaaga ctagattttg ctcggcaaat cttctgcagg tgtcctgatg
     1321 ctcgccacca aggatagcaa cagctctttc ccacgaatgc tcgggcacgt aacttgcaga
     1381 attccaggtg tagtcagcac aggtaagaag aggcagtttg gacagttccg cctcgttcat
     1441 ggggttgaag gtaaagccca aagtgcccgg aagggtcgga cctcttccgg caataggggc
     1501 cataaacagc ttggttcggt tgtagtcgtt tacaggatag ttatcccagt aaacaatgtc
     1561 atgtccccaa atctcgtaga tctccccagc ttcttttgga gtaatggttt gggcaactat
     1621 accgtagcct gtggagtaga cgatgatatc cggcgacagt ttggtgttat aagttctgcg
     1681 gtagggcgag gtgccttcct ggtagtattc ggtgggaaca actatcagcc tatgacccgg
     1741 tatcttagcc gccaggtaat cctggtatct attacataga tctacttgag caagagcata
     1801 gtcgttgccg tattttgccc tgtcccgtgc agaacgcaga ttggggtcga tgtcgtctag
     1861 gagcagggcg aagtgtgtaa tgccgatttc gatcatggcg tcggttttcg cgagcagctt
     1921 ctgccagtcg gattctgaag aaaactcgat ggaaagacct ggagagatgg caaacacgaa
     1981 gtctacatag ctggccttgg cagcttgcac tagttctttt agctgcgcaa gtttttcatc
     2041 gggataaggc tcccgccatt tttcgcggtg atagggatcg tctttgggag cgtagacgta
     2101 ggtgttcatt ttgctttcgc caagaaagcg gaccatgtcc actctagcag cgtggctcca
     2161 gggatcgtga tagaaaccct caataacgcc tctgatctgg taatccgggg catcgatgat
     2221 ggttacctcc ggaagctcta ctgcatcttt gatgagttgc ttgaaagtta tgagagcgta
     2281 gtagagagct cgctcgctaa ttgccactat tctgcctcct tcatcggtga cttcgatgag
     2341 ataggcctcg tttccgtagt gggaaagctc aactgtttct ccgatggtga cactgatctt
     2401 gcgcggtgca tcttcgcttt caacaatgct gaactgaaac ttatccttta gatctgcatc
     2461 aactttccac atcaacgaga aactttcgag aggaaaggat tcagttccca aaatcaaggt
     2521 ttgcggagta gggaagatgt cgtagacttt tgcatgtact atttgcacca agagcaaaac
     2581 aagaatgaaa acggcaagac gcttagcctg cataaacacc acctccacca ttgttaccta
     2641 gaataaaagt tcaagtccgg cggatagatc cctttagatc agagcggata tgcgatttgg
     2701 tataggccgg aagtaaaagg agaaaaaacg gccatttcca agaactctct ctgtagggca
     2761 aggtatttta cttattacta aattttgcca aatgaggaca aacttgacgg ggcttgtcaa
     2821 ctttgttact atatttgcgt ggtcttactg tggcagcaga agatccctca gccttatgca
     2881 ccgcggggta gggcgtgtca taaggtaaat ttaactgagg aggatttgac ctgaaatggc
     2941 aaattcaaag gtaacaaaga agccgagcgc aagtagtggt gacaacttct tcgaaagagt
     3001 gttcaagcta aaagagcaca acaccaccat tggcatcgag gttatggctg ggttaacaac
     3061 gttcatgacc atggcgtaca ttattgctgt caaccctggt atgcttgctg aaactcagca
     3121 ggttagcttc gacgctgcac tcattgcgac ggtgctatct gcagcattcg ctactttctt
     3181 aatggcattt ttagcaaact atccatttgc gctagcccct ggcatgggtt tgaacgctta
     3241 ctttgtttat acagtcatta tgggtatggg atatacttat gctcaagcac taacagccgt
     3301 tctcatttcc ggtattgtgt tcgtcatcct gactgttacc ggactgagac aggttattgt
     3361 gaaagcgata cccaataacc ttaaggctgg tattggtgcc ggtattggtt tgtttatcgc
     3421 attcttgggc ttgaaaaacg caggaattat ggttagcgat tctgcaacat tgttaggttt
     3481 gggagacatg acttccgcac ctgtaattct tgctattgtt ggcttgatcg ttacggcagt
     3541 gttgatgtac aagaacgtca aaggcagcat tttgatcggt atggcggtaa ccaccgttct
     3601 tgcatggatt acaaaggtag ctcctggacc agcaggcatt gttgcatggc cgaatcttgg
     3661 agcttggagt tctgaagctc tctttaaact cagtggcttc aagacccttg gtatcttggg
     3721 agttgtcgag gttgtctttg cattcttctt cgtcgatatg tttgacacta ttggaacttt
     3781 ggtcggtgtt gcaaagagaa gca
//
LOCUS       DAOSDA010000130         3786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15329_length_3786_cov_3.167515, whole genome shotgun sequence.
ACCESSION   DAOSDA010000130 DAOSDA010000000
VERSION     DAOSDA010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15329_length_3786_cov_3.167515"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      721..886
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            945..1700
                     /gene="dapB"
                     /locus_tag="PLW82_04015"
     CDS             945..1700
                     /gene="dapB"
                     /locus_tag="PLW82_04015"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOJ46473.1"
                     /translation="MSKLRVGVIGAGGRMGREVIKAVSSDAELMLTAACDPAFAGEDA
                     GRFSGLGESGVIIDGSLQDAVMKADVWVDFTRPDTVKGNIATAVEAGKPIVVGTSGLS
                     ESYVSSLVTNNVGVLVVPNFAIGAVLMMHFAKIAHKFFPSVEIIELHHDKKIDAPSGT
                     AAKTAELMGAKEQGVDEGPRGLNRDGIHIHSVRLPGLVAHQEVIFGGLGQILTIRHDS
                     LDRSSFMPGVILAIKEVVKLNGPVYGLEHVLGL"
     gene            1819..2832
                     /locus_tag="PLW82_04020"
     CDS             1819..2832
                     /locus_tag="PLW82_04020"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOJ46474.1"
                     /translation="MRKVKVAVLGATGAVGEELLELLIERNFPFSEIRALASKRSAGK
                     VLEVKGHKFTVEEATPEAFSGIDIAFFCAGGSVSQQLAHEAVKRGAVVIDNTSAFRMD
                     KDVPLVVPEVNPEDVKWHKGIIANPNCSTIIMTVALGPLEKHFGLDRIIVSTYQAVSG
                     AGVKGMEELRTEVEDPDYEAQVLPVASLPKHYRIAYNLIPQIDVFDKEGYTKEEWKMV
                     NETRKMLHREDLGITATTVRVPVMRCHSESVYVELKKDFSLDEVRKVLSEAKGVQIQD
                     DIENQIYPMPLFLEKKDDIYVGRIRRDLHNPRGLNLWIVGDQVRKGAALNAVQIGEIL
                     LAH"
     gene            2860..>3786
                     /locus_tag="PLW82_04025"
     CDS             2860..>3786
                     /locus_tag="PLW82_04025"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOJ46475.1"
                     /translation="MKFGGTSLKDRECRDAAANHIISTPATPVVVVSAMGRNGAPYAT
                     DSLRQLLISEGGEISDRDMDFIMCCGEMISAAVLAQAIKARGRRAKPLTGAQAGISTD
                     GVYGSATVTGVDVAPIRKLLADGIIPVVTGFQGDFAGEFTTLGRGGSDTTAAVLGQAL
                     GADEVQIFTDVVGILTTDPVLYGEASFVPFATYQEVLEMAELGAKVIHPKAVEILESA
                     GIPIRILSTLEKGPGTYVSAPVDPSPLKKKKVVTSVAVAYPRSLVSIKDNQADVVFKT
                     VAEANVSVDLINVSPMSISFTIDSDREQTVRKV"
BASE COUNT          978 a          849 c         1051 g          908 t
ORIGIN      
        1 gctatcgtca catggtagaa gtcgtgggtt cgagtccctc attgcccacc atattttttg
       61 ttaataaagc ggtgtaggac ccagttggat gctctacacc gcttttgtat tgcatgagac
      121 tgtttgcagt ttcggaagtc ctccttctgc atagcctttt tccttttgct ggtgtatgga
      181 gagctcttga acggagcctg caaacccagg tgccgagaag ttgtttgcct ccttcttcgc
      241 agggtataat agatgtgaaa atagacttcc aggccatttt cggtccgctc tggtagaacg
      301 gggcctttgc gtaatcaatg gtggaggagg tctatggcag gaaggaagac ctattgtttg
      361 atgagatttt cgaactaatc ttgaatggaa gaattgacga agccaaagaa aaaagcaaat
      421 gcctctttga tgatgagcgg gtgcagttgg cgcgaaccgc agcaatatcc gagacttcat
      481 atactgccta cagttatctt cgcacactca ttttggaaga agacaaaggt atctatctaa
      541 gacatctatg taggtcgtgt catcgtcact agactaccat caagaggcca ggaagcgaat
      601 tcattaccta atcacagctc aacaaggaac tacagcgatc cctacaaaac ttgaacgcga
      661 tcaatagggt taatgctctt gacaggggca ccttgctgtg ggaaaataaa cgaaataaat
      721 aagtgagcta gaggcgcggt aagcaagagt acttagggga agcggcagct gatgaagcta
      781 aggaaagggg agtaccgccg aagtcaagac ggggcaccgt tttggctggt tagccagtga
      841 ataactgcct aactgtcaca agtaattgtg atgagctagc ctgtttgacc agttaaggtc
      901 ggcatgttag ctgccggctt ttttcatttt tggaggtggt ttgtctgtct aaactacgag
      961 ttggtgtaat tggtgctggc ggacgaatgg gacgcgaggt cataaaagcg gtgtcttccg
     1021 atgcagagct gatgctcact gccgcttgcg atccggcgtt tgcaggagaa gatgcaggtc
     1081 gattctccgg tttgggagaa agcggagtga tcatagatgg ttctctgcag gatgctgtaa
     1141 tgaaagccga tgtttgggta gacttcacca ggcctgatac tgttaagggc aacatagcaa
     1201 ctgctgttga agcgggaaag cccatcgtcg ttggaaccag tggactcagc gagagctacg
     1261 tcagctcttt ggtaacaaac aacgttggcg tactggtcgt tcccaacttc gctatcggcg
     1321 cagtgctgat gatgcacttt gccaaaatcg cacataagtt ctttcctagt gttgagatca
     1381 ttgaactaca ccacgacaag aagatcgatg ccccgtcggg gactgcagca aagactgcag
     1441 agctgatggg tgctaaagag caaggcgtcg acgagggtcc tagaggcttg aaccgcgacg
     1501 gtatccatat ccacagcgtt cgccttccgg gactggtagc ccatcaggag gttatcttcg
     1561 gcggacttgg gcagatcctt acaatcaggc acgattcttt ggacagatct tccttcatgc
     1621 ccggtgtgat cttggccatc aaagaagtgg tgaagctgaa cggaccggtc tacggcttgg
     1681 agcacgttct aggactgtaa ctccaatcta ctttttggca tatgatgtgt cggctccgga
     1741 gaagatgtcg aaacggttga ggtggcgctt aacggcaaat accacgatag ttacctactg
     1801 ataaggagtt gattgaaagt gagaaaggtc aaagtggcgg ttttaggggc aacaggagct
     1861 gttggcgagg agcttctaga gctgctcata gagagaaact tcccattcag cgagattcgg
     1921 gctttggcgt caaagagatc cgctggaaag gttttggagg tcaaaggaca caagttcaca
     1981 gttgaagagg ccacgccaga agcgttttcc ggcattgata ttgcgttttt ctgtgccggt
     2041 gggagcgtgt cccagcagct cgcccatgaa gctgtaaagc ggggtgccgt agtgatcgac
     2101 aacacttccg cttttcgaat ggacaaagac gttccactag ttgttcctga agtaaatcca
     2161 gaggatgtca agtggcacaa aggtattatc gccaacccta actgctcaac gatcatcatg
     2221 accgttgctc tcgggccttt ggagaagcac tttggcctag atcgcatcat tgtctcaact
     2281 taccaagctg tatccggagc aggcgttaaa ggcatggaag aactcaggac cgaagtggaa
     2341 gatcccgact atgaagctca ggttcttccg gtggcttccc tgccgaagca ctaccggata
     2401 gcctacaacc tcattcctca gatcgacgtc ttcgacaaag agggctatac taaagaggaa
     2461 tggaaaatgg taaatgagac ccgtaaaatg ctgcacaggg aagacctagg cattacagca
     2521 accaccgtta gagtaccggt aatgcggtgc cactctgaat cggtttatgt ggaactgaag
     2581 aaggacttct ccttagacga ggtgagaaag gtcctctcag aagccaaagg ggtacagatc
     2641 caggatgaca tcgaaaacca aatctacccg atgccccttt tcctagagaa aaaggacgat
     2701 atttatgtcg gccgcattag gagagatctg cacaacccca gaggtttgaa cttgtggata
     2761 gttggtgatc aggtcaggaa aggtgctgca ctcaatgcag tccagattgg agaaatcttg
     2821 cttgcacatt gaggtgaagg catggaaaag cttttggtaa tgaagtttgg gggcacgtcg
     2881 ctgaaggata gagagtgcag agatgcagct gcgaatcaca tcatcagcac tcctgccacc
     2941 ccagtggttg tggtttctgc catgggaaga aacggtgcac cttatgctac cgattccctc
     3001 cgccagctgt tgattagcga aggtggggag atttccgatc gggacatgga ttttattatg
     3061 tgctgcggcg agatgatttc tgctgcggtt ctggcccagg ctattaaagc cagaggacga
     3121 agggctaaac ccctcacagg agcgcaagca ggaatcagta cagacggcgt ctacggtagc
     3181 gctacagtca caggtgtgga tgtagcgccg atcagaaaac tcttggcaga cggcattata
     3241 cctgttgtca ctggtttcca gggagatttt gccggcgagt ttacaacctt gggcagaggc
     3301 ggcagcgaca ctaccgcagc ggtgttaggt caggcactag gtgcagatga ggtgcagatc
     3361 tttaccgatg tggtgggcat ccttacaaca gatccggttc tgtatgggga ggctagcttt
     3421 gtgccctttg cgacctacca ggaagttttg gaaatggcag agctgggagc aaaggtcatc
     3481 catcccaaag cggtagagat cttggagagt gcgggaattc ctattcgcat cttgagcact
     3541 ttagagaagg ggcccgggac ctatgtctcc gctccggtgg atccgagtcc cctgaaaaag
     3601 aaaaaagtgg tgactagcgt agctgtggcg tatccccgct cactggtttc catcaaggac
     3661 aatcaagcag acgtagtttt caaaaccgtc gctgaagcca acgtcagcgt cgacctaatc
     3721 aacgtgtctc ccatgtctat ttccttcacc atcgactccg acagagaaca gacagtccgc
     3781 aaagtc
//
LOCUS       DAOSDA010000131         3778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15375_length_3778_cov_3.183991, whole genome shotgun sequence.
ACCESSION   DAOSDA010000131 DAOSDA010000000
VERSION     DAOSDA010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15375_length_3778_cov_3.183991"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..562
                     /locus_tag="PLW82_04030"
     CDS             197..562
                     /locus_tag="PLW82_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46476.1"
                     /translation="MRRLMFAAALIGVLAASVFAANIEIWMFGMANEYVKIHQELIEQ
                     EFTPKTGITVNVLAYPSEGFQNKVLLAMVSGDSPDIVTGPVDHIVEYGIRGSILPLRK
                     VFPEKLAEIEPRLYSHPLG"
     gene            564..752
                     /locus_tag="PLW82_04035"
     CDS             564..752
                     /locus_tag="PLW82_04035"
                     /inference="COORDINATES: protein motif:HMM:NF028444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5109 domain-containing protein"
                     /protein_id="HOJ46477.1"
                     /translation="MEIFDIKTWRPAPIELLKRQLLAVEPHVEKVIIFEFNHYMNPYR
                     SIHTRVLYEDYKAWIEEK"
     gene            791..>1832
                     /locus_tag="PLW82_04040"
     CDS             791..>1832
                     /locus_tag="PLW82_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46478.1"
                     /translation="MKRQLLVSILVLVLSLPSFAVTIDVWLQHTNEEMKILREITDKF
                     TEKTGIEVNYTYVNPADSETVFLMAAASGTAYDVGGVGSLFAPELGLRGAVINLAQFP
                     DYEEFAANFPPMYFNSLQYQGLVFGIPYRSYLYLTYQRTDILQDLGIAPVTTWTELKS
                     ILPRLQAQGMNFALTFGISESVYADVNMFMWQYGGDDYNEDLTKSGYDSPESIAGFKE
                     FAEIYTQYKIPQQVVAYQSFTQGELPILMQSGHFATNLEVSAPQLAGKWQMTPAIGTM
                     RDGELHIESFIGGVALVISKDSKNPQAAWEYIKWLCSVETQTEITTRLAQQIRGNLFI
                     PANSEAIGNMPVG"
     assembly_gap    1833..1842
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1843..2092
                     /locus_tag="PLW82_04045"
     CDS             <1843..2092
                     /locus_tag="PLW82_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46479.1"
                     /translation="EENALTLRKQAEGSRASMYGLVAPRLRRRYLQFAAEEVVLLGRD
                     PEEAIRKAAADHNEEIRKKTIEYDRFIKRMLEEAGKAK"
     gene            2136..2864
                     /locus_tag="PLW82_04050"
     CDS             2136..2864
                     /locus_tag="PLW82_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46480.1"
                     /translation="MKRTLGLILIVLLLVSATIYAKKPLAVVYSEKTANATVNYPTYK
                     GDFDFFTRWLSKFAEFDVITDADVEAGALKDYKAVLLPDNAVMSEEEAEYYLDFMDRG
                     GRVFGCNSTSVRNEELQLTKYLLADVFGVTWVQWVNDPSKHNYVVFTDHPIFEGLDVD
                     HVQLLTSATQVVNNQDGEAIATWVNADLTTPSEPEERNAAFFEYEGNIFVTFQIIKSI
                     YLNHKVYDQIMQNIVAYLAPDAIK"
     gene            3076..>3778
                     /locus_tag="PLW82_04055"
     CDS             3076..>3778
                     /locus_tag="PLW82_04055"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOJ46481.1"
                     /translation="MKTFITEDFLLYNDTARELYHETAKDLPIIDYHNHLDPYAILED
                     RKYKNLTEIWLGDHYKWRLMRANGIGEEYITGERSDYDKFLAWAKTVPATLGSPLYHW
                     SHMELLRHFNIDVLLNEETAPFVWEEANRKLESGEITPRKILEKFKVEFVGTTDDPAD
                     PLDAHQKLRQEGYGVFVAPSFRPDRALGIEKEDFTAWVERLSKVTNSNIDTYDKFLEA
                     LERRVDFLDSLGCRSS"
BASE COUNT         1050 a          936 c          917 g          865 t
ORIGIN      
        1 aaacagcacc tcgaaagact cttggctatt cgccgagacc agagactctc acctacgact
       61 aaagcaaaac tacatatcat tcctaagcgg aaggaaacgc ggtttttgac tgcctagagc
      121 gtacgctctc cgggaagcta gaaagatgct ttagtctgtt gctttgaaaa tcaatctgaa
      181 aagaaggtgg gacatgatgc gtaggttgat gtttgctgca gctttgatcg gtgtgcttgc
      241 agcgtccgtt tttgctgcga acatcgagat ctggatgttt ggtatggcca acgagtatgt
      301 gaagattcac caagagctga tcgaacagga attcactccc aaaaccggga tcaccgtgaa
      361 cgtcctggcc tatcccagtg agggcttcca aaacaaggtc cttctggcga tggtttcagg
      421 ggattcacca gacattgtca cgggtccggt agatcacatt gtcgagtacg gtatcagggg
      481 atcgatcctg cccctccgta aggtcttccc tgaaaaactt gccgaaatcg agccaaggct
      541 ttacagtcac cctctgggct gatgtggaaa ttttcgacat caaaacctgg cgcccagcac
      601 ctattgagct tttgaaaaga cagcttttgg ctgtagagcc tcacgtcgag aaagtaatca
      661 tcttcgagtt caatcactac atgaatccct acagaagcat tcacaccaga gtactctacg
      721 aagattacaa agcatggatt gaggagaagt aacgtcaggt cattgattca aaaccttcgg
      781 aggtgtttgc atgaaaagac agctacttgt ttcaatcctt gtacttgtcc tgtcacttcc
      841 tagttttgca gtcaccatcg atgtgtggtt gcagcatacc aatgaagaga tgaagattct
      901 tagagagatc accgacaagt ttaccgaaaa aaccggtatt gaggttaact atacctacgt
      961 caaccctgca gacagcgaaa cggtgttcct catggcagct gcaagcggca ccgcctatga
     1021 cgtaggaggc gtaggatcgt tgtttgcccc tgagctgggg ctacggggtg cggtcattaa
     1081 cctggcccag tttcccgact atgaagagtt cgctgcaaac ttcccaccaa tgtacttcaa
     1141 ctcactgcag tatcaggggc ttgtctttgg cattccctac agaagctacc tctaccttac
     1201 ctaccaacgg acggacatcc tccaagactt agggatagct cctgtaacca cgtggaccga
     1261 actgaaaagc attctaccca ggctgcaggc ccaaggcatg aacttcgctc tcaccttcgg
     1321 catctcagag tcggtctacg ctgatgtcaa catgttcatg tggcagtacg gaggcgacga
     1381 ctacaacgag gatctgacca aatccggcta tgactcgcct gaatccatcg ccgggtttaa
     1441 agagtttgcc gagatctaca cccagtacaa gattcctcaa caggttgtgg cctaccagtc
     1501 ctttactcaa ggtgagctgc ccatccttat gcagtcgggg cattttgcca ccaacctcga
     1561 ggtaagcgca ccgcagcttg ccggaaagtg gcagatgact cccgccatcg gtaccatgag
     1621 agacggtgag ctccacatcg agtcgtttat cggcggcgtg gctctggtta tatccaaaga
     1681 cagcaagaac ccgcaggcag cttgggaata catcaaatgg ctatgcagcg tagagactca
     1741 gaccgaaatc accacaagac ttgcccagca gatcaggggc aacctcttca tccctgcaaa
     1801 cagcgaagct atcggcaaca tgccagtcgg cgnnnnnnnn nncgaggaga acgccctgac
     1861 acttcggaaa caagccgaag gttccagagc atccatgtac ggacttgtgg cgccaaggct
     1921 tagaaggcgt tacctgcagt ttgccgctga agaagtggtg cttttgggaa gagaccctga
     1981 agaggccatc aggaaagctg ctgcggacca caatgaagag atccgcaaaa agaccatcga
     2041 gtacgatcgc tttatcaaaa gaatgctgga agaagcaggt aaagccaagt agatcagagt
     2101 agatttttgg tgaaaaggtt aaagggggaa tgattgtgaa aagaacgttg ggtttgattc
     2161 tcattgtctt gcttttggtt agcgctacca tctacgccaa aaaaccactt gcggtagttt
     2221 actcagagaa aaccgcaaac gccacggtta actatcccac ctacaaaggc gactttgact
     2281 tcttcacaag atggctcagc aagtttgccg agttcgacgt cattactgat gccgacgttg
     2341 aagcaggagc ccttaaagac tacaaagcag ttcttcttcc cgacaatgcg gtaatgagtg
     2401 aagaggaagc cgagtactac ctggacttca tggatcgagg cggaagagtt ttcggctgca
     2461 actccacatc agtgcgcaat gaagagctgc agctcaccaa gtacctgctc gcagacgtct
     2521 ttggcgtaac ctgggtacag tgggtaaacg atcccagcaa gcacaactac gtagtcttta
     2581 ccgatcaccc catctttgaa ggtcttgacg tagatcacgt gcagctgctt acatctgcaa
     2641 cgcaggttgt aaacaaccag gacggcgaag ctattgctac ctgggtgaac gcagatctta
     2701 caacgcctag cgaacctgaa gaaagaaacg ctgcattttt cgagtacgaa ggcaacatct
     2761 tcgtgacctt ccagatcatt aagtccatat accttaacca caaggtatac gatcagatta
     2821 tgcaaaacat agtggcttat ctagctccag atgccattaa gtaaacaaga aactaacgct
     2881 tccactgcca agcggtggag gcgttttctt ttatcccctt ttaacctgca agatgtagcc
     2941 caaaaacacc ctctcctaag aggaggttta tctttttttg ggcaggattt ggctctaagg
     3001 tggtataaaa aggtaaggga caaagagact ttcaagaggt cctttttatt tcaaccaaaa
     3061 aagaaggtga tttttttgaa aacattcatc accgaagact ttctcctcta caacgatact
     3121 gcaagagagc tgtaccacga aacggcaaag gatcttccca ttattgacta ccacaaccac
     3181 ttggacccct atgctatctt ggaagataga aagtacaaga acctcacaga gatctggctc
     3241 ggagatcact ataaatggcg tctgatgagg gctaacggga tcggagaaga atacatcaca
     3301 ggggagagat ccgactacga caagttcctg gcatgggcca agacggttcc tgcaactcta
     3361 ggaagtcccc tgtaccactg gagccacatg gagctcctta gacacttcaa catagacgtt
     3421 ctcctaaacg aggagactgc cccgtttgtc tgggaagagg caaacaggaa gctggagtcc
     3481 ggcgaaatca ctccccgcaa aatcctggag aagttcaagg tggagtttgt tggaaccaca
     3541 gacgatcctg cagatcctct agatgcacac caaaagctgc gccaggaagg ctacggcgtt
     3601 ttcgttgccc cttcgttcag gccggacaga gctttgggca tagaaaaaga ggactttacc
     3661 gcatgggtag aaaggctctc taaagttaca aactcaaaca tagacaccta cgataagttc
     3721 cttgaagctc ttgagaggcg agtggacttc ttggacagtc tcggctgccg cagctcgg
//
LOCUS       DAOSDA010000132         3756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15502_length_3756_cov_2.725750, whole genome shotgun sequence.
ACCESSION   DAOSDA010000132 DAOSDA010000000
VERSION     DAOSDA010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15502_length_3756_cov_2.725750"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="PLW82_04060"
     CDS             complement(<1..1375)
                     /locus_tag="PLW82_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOJ46482.1"
                     /translation="MHYRKAIIALQLFRRMINRMNNPWVIGGVSAYILMMLVIGWLSS
                     KNIKDATDYIVAGRRLPLGLATAALFATWFGGGTSMGAAGESYWNGFLGTIADPFGAG
                     LCLLLAGLFYVRTMRRMKLLTIADFFAIKYGKTSELLVALITLPAFIGWVGAEMVALG
                     LIVHSVTGLTPTAGIIISAIVVLIYTYQGGMWADTLTDFAQMIILVAGLVIMFPIVIN
                     HVGGFSAIKAQIPDEMFYFFPRNTGFSGWMFYLQAWIIVGLGSIPGQDVMQRALSSKD
                     ENTAQWASYLSGLGYMLLGIIPVTLGIVGRLVFPELANSELVLVELAMKFLPNFVVAL
                     FVGALLSAIMSSTDSAILAPASIVTRNILPHIKKMDGKGELKVARIATVVITALALAT
                     ALWFQNVYDLMVSAWGFLLVGCFVPLTFGIWSKKSNTPAAVTSSIIGFVSWIILGIVS
                     PNTPNDLI"
     gene            complement(1565..2293)
                     /locus_tag="PLW82_04065"
     CDS             complement(1565..2293)
                     /locus_tag="PLW82_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOJ46483.1"
                     /translation="MDRLSKKSLLRTSLIIGESALEKLAKSRVIVCGVGGVGGAAAEA
                     LARSGVGSITIIDGDVVDITNLNRQIVATLENIGRDKAFEMAKRLKSVAEDVDVHPIK
                     VMLTDENIPEYIKDADYVVDCIDDVDAKTALICYCLKNQIPIVSAMGAGGKLDPSFMR
                     VGDISETKVDPLSKVMRRRLKLNGITSGLKVVYSLEPAVKAKEVDGKRVIGSSSFVPP
                     AFGLTLASLVVRELTNELLEKENA"
     gene            complement(2398..2578)
                     /gene="ssrS"
                     /locus_tag="PLW82_04070"
     ncRNA           complement(2398..2578)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLW82_04070"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2646..>3756)
                     /gene="aspS"
                     /locus_tag="PLW82_04075"
     CDS             complement(2646..>3756)
                     /gene="aspS"
                     /locus_tag="PLW82_04075"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOJ46484.1"
                     /translation="FRDEDLRKDRQPEFTQIDIEMSFIDEEDIYDLIERMFVYVFDKV
                     LGIKLDAPFPRMPYAEAMERFGSDKPDTRFGMELVDLSDIARECGFKVFRDAVENGGQ
                     VKGLLVKGCADYTRKQLDDLTLLAQELGAKGLAYILYKEDEVKSPIVKFFSEEEIGRI
                     LERMNAEKGDLILFVADKKEVVAAVLGRLRLDLGEALGLIPHDKMNFLWIVDWPLLEW
                     DSDEKRYVAVHHPFTSPKSPDFDKDPGNALAKAYDLVLNGVELGGGSIRIHDAEVQSR
                     LFRTLGISDEEAEEKFGFLLKAFKFGPPPHGGIAFGLDRMIWLLAGADSIRDVIAFPK
                     TQKATCLLTEAPSSVDPRQLRELHIASTVVKEKME"
BASE COUNT          971 a          978 c          879 g          928 t
ORIGIN      
        1 cgatcaagtc gttaggtgtg ttaggcgaaa cgatgccgag aatgatccaa gaaacaaagc
       61 cgatgatgga agacgtaact gccgctggag tattggactt cttgctccaa atgccaaatg
      121 tgagtggcac aaagcatccg acaagaagaa agccccaagc ggaaaccatt aggtcgtaga
      181 cgttctggaa ccaaagtgct gtggccaaag ctaaggcggt aatcactacc gtcgcgattc
      241 ttgccacttt tagttcgcct ttgccatcca tcttcttgat gtgtggcagg atgttccttg
      301 taacaatgct tgcaggcgcg agaatcgcgc tgtctgttga agacatgatg gctgaaagca
      361 gagcgccaac aaaaagagca acaacgaagt ttggcaagaa cttcatagct agctccacca
      421 ggaccagctc cgagttagca agctcgggga aaaccaacct gcccacgata cccaaggtta
      481 ccggaatgat cccaagaagc atgtagccca aacctgaaag atacgatgcc cattgggctg
      541 tgttttcatc tttactggaa agagctctct gcatgacgtc ttgtcccgga attgatccca
      601 gaccaacgat aatccaagct tgcaaataga acatccatcc cgaaaatccg gtgtttctcg
      661 ggaagaagta gaacatctca tcggggattt gagccttaat agccgaaaaa ccaccaacgt
      721 ggttgataac gatgggaaac attatgacta gacctgcgac gagaataatc atctgggcaa
      781 agtcagtgag agtgtccgcc cacatgccgc cctgataggt gtagatcaaa acaacaattg
      841 ctgaaatgat gatgccggct gtcggggtaa ggcctgttac ggaatgaaca atcaacccta
      901 aagcgaccat ctctgcgcct acccatccga taaaggccgg aagcgtgatc agagcaacca
      961 gaagttcgct ggtttttccg tatttgatgg caaagaagtc tgctatggta aggagcttca
     1021 tgcggcgcat agttctaacg tagaaaagac ctgcaagaag aaggcagagt ccagcaccaa
     1081 agggatctgc tatggtgcct aaaaagccat tccagtacga ctctcctgcg gcacccattg
     1141 atgtgccgcc accaaaccac gtggcaaaga gtgcagcggt agcaagacca aggggcaacc
     1201 gccttccggc aacgatgtag tctgttgcat ctttgatgtt tttgctagaa agccaaccga
     1261 ttacgagcat catgagaatg taagccgaca ctccgccgat tacccatgga ttgttcatcc
     1321 tgttgatcat cctcctaaat agttgaagtg caatgattgc tttgcgatag tgcatctgag
     1381 tttgctcctc taaaaggctc gaaaaaggag gattttcgag gaaaacgcac cttacttgcg
     1441 ctatctcaaa atgtcttacg aaaattgatt atgaggcata cacaggcaag agtcaatacc
     1501 ccgaaaataa aaaacggcta gaaatttttc gcttaaatct ctagcctgaa atagctcagc
     1561 tttctcatgc gttttccttt tctaaaagtt cgtttgttaa ttctctaaca actagcgaag
     1621 ccagggttag tccgaatgca ggcggtacaa acgaactaga cccgattacc cgcttcccgt
     1681 caacttcttt agctttgacc gcaggctcta gcgagtacac caccttcaat cctgaggtga
     1741 tgccgttcag cttcagccta cgcctcatca ccttactcag agggtcaact ttagtctccg
     1801 agatgtcccc tacgcgcatg aaagacgggt ccagtttgcc ccctgcaccc attgccgaga
     1861 cgatgggaat ctggttcttc aggcagtagc agatgagagc ggtctttgca tcaacgtcat
     1921 cgatacagtc cacgacgtag tcggcgtctt tgatgtactc ggggatgttc tcatctgtta
     1981 gcatgacttt gataggatga acatccacgt cttctgcgac gcttttcagc ctttttgcca
     2041 tctcgaaagc cttgtctcta ccgatgttct ccagcgtggc aacaatttgt ctgtttaggt
     2101 tggtgatatc cactacatct ccgtcaatga tggtgatcga gccaactccc gatcgggcta
     2161 gagcctctgc tgcagctcct ccaaccccgc ccacaccgca gacgatcact ctactttttg
     2221 caagcttctc aagggcgctc tcgccgatga tcaagcttgt tctgagcaga ctctttttac
     2281 ttaatctatc cacaaactcc aactcctatt tctgcgtaaa tcaaaaccgt atcgctgttt
     2341 ttaccgaact cttttttaga ttagcacaaa aaagggagcc gtaaaaggct cccacgcgta
     2401 tcccccgcca tgccgctggt gctgaaagtt aatgaacctg ctcgggcagg tgggtgtcct
     2461 gttcacagct taggcaaagc cgaaaccggc tcggcggcca ctgcaaaacc ccggactccc
     2521 gttttgtgtg tgttggctca taaacacttc agcttgacca cgaacacagc agggtggtgt
     2581 ctgcaattat cttagcacaa aaatggcgtt ctttcaatga cagaggcggt ctttcgactt
     2641 gaagcttatt ccattttttc cttgacgaca gtggaagcga tatgcagttc gcgtagctgt
     2701 ctaggatcaa cagacgatgg tgcctctgtc agaaggcagg tagccttctg agttttgggg
     2761 aaggcaatga cgtctctaat ggagtctgcg cctgctagaa gccaaatcat gcggtcgagt
     2821 ccgaacgcaa tacctccatg aggtggagga ccgaacttaa acgctttgag caggaagccg
     2881 aacttttcct ctgcttcttc atcggagata cccagagtcc taaagagcct ggactgaacc
     2941 tcggcatcat ggattctaat ggagccaccg cctagttcta caccgttgag gaccaagtcg
     3001 taggcttttg ccagagcatt tccgggatct ttatcgaagt ccggactctt tggagaggtg
     3061 aacgggtggt gcactgccac gtagcgcttc tcatcgctgt cccactctaa aagcggccag
     3121 tccacgatcc agaggaagtt cattttgtcg tggggtatga gacctagagc ctctcccaga
     3181 tcgaggcgaa gcctgccgag cactgcagct acaacttcct tcttgtctgc aacgaacaag
     3241 atgaggtcgc ctttttctgc gttcatgcgc tcgagtatcc ggccaatctc ctcctctgag
     3301 aagaacttga cgatgggact cttcacttca tcttctttgt agaggatgta tgcaagacct
     3361 ttcgctccca actcctgtgc aagaagagtc aggtcgtcta gctgctttct ggtatagtca
     3421 gcacatcctt taaccagaag tccctttacc tgtccgccgt tttctacagc atcccggaag
     3481 actttaaaac cacactccct ggcaatgtcg gaaagatcga ccagctccat accaaagcgg
     3541 gtatcgggct tgtctgagcc gaatctctcc atggcctcgg catagggcat tcttggaaat
     3601 ggcgcatcca gcttgatgcc tagcacctta tcaaagacgt atacaaacat cctctcaata
     3661 agatcgtaga tgtcctcttc gtcgataaac gacatctcaa tatcaatctg ggtgaactca
     3721 ggctgccgat ccttccttaa gtcttcgtcg cggaag
//
LOCUS       DAOSDA010000133         3743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15578_length_3743_cov_3.313178, whole genome shotgun sequence.
ACCESSION   DAOSDA010000133 DAOSDA010000000
VERSION     DAOSDA010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15578_length_3743_cov_3.313178"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /gene="gcvPB"
                     /locus_tag="PLW82_04080"
     CDS             <1..908
                     /gene="gcvPB"
                     /locus_tag="PLW82_04080"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOJ46485.1"
                     /translation="AAMCGFKVIEVKSKDGMVDIEELRQKVSDKTAALMITNPNTLGL
                     FEKNICEIEKIVHDAGGLIYYDGANLNALMGIVRPGDMGVDVMHFNLHKTFSTPHGGG
                     GPGSGPVGVKKHLEPYLPRPVVVKGEDGFKLDFDRPKSIGKVASFYGNFGVVLRAFVY
                     MTVLGKCGLRKASELAVLNANYLKALLEDVLDVPYSEGTMHEFVATSKDTDLTALEIA
                     KSLLDYGYHAPTVYFPLIVHEALMIEPTETESPETLEEFAETLKEILKNPERSKSAPH
                     LMPIKRADEVKAARHPVLTRSQIAE"
     gene            945..1715
                     /locus_tag="PLW82_04085"
     CDS             945..1715
                     /locus_tag="PLW82_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ46486.1"
                     /translation="MKSMKFYEPKTISVSLTGKNCGLNCKYCQGHYLQHMVSAKELKV
                     EEDASYLISGGFDRAGRLPLYENRDLLRNLCQKAKLNVHPGFLKDEDLELLKEIDPVV
                     SFDFIQDPKVIREVINLPYGPEDYVNQYKIMVDAGLKVVPHMLLGLGEKDSETLQALA
                     ELQPEKAVVLVFRPTRGTSLAGKELPSLRELGAILDDFRKAYQGELILGCMRPGGTYR
                     IELDNLAADLGFDGIVKPVKRVKDLLKDAEIFDECCAF"
     gene            1728..2603
                     /locus_tag="PLW82_04090"
     CDS             1728..2603
                     /locus_tag="PLW82_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ46487.1"
                     /translation="MRASIGTAGVLGLKRLKQICPPKTAYLLTDGQCRRNCKFCGNSN
                     QLARVTWPHFAKEEIISSLKSTDMKRICIQTVDSKEALEEVREVLPEIIKTGIPVCLS
                     SQFGEEFLEMGLDHWTIPLDVVRPDQYTAIKGGSFEKALDRIKAMAAKYPNRIGTHLI
                     AGLGETEEEMITLLHDLYRSGVSVGLFAFTPVPGTELENHPKIPLDSYRRIQIGNYLV
                     SKYPEAIEEMVFQEGRLVFAPLDKVESRAFETWGCEDCNRPFYNESPGGPWYNYPRPL
                     SPEEFREALAESGLK"
     gene            2811..>3743
                     /locus_tag="PLW82_04095"
     CDS             2811..>3743
                     /locus_tag="PLW82_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ46488.1"
                     /translation="MKTSKKLTEKVTWVGKVDWELSSFHGEELSTHRGSSYNAYLIRD
                     EKVALIDTVWVPFDKEFVKNLKSEIDLKEIDYVIIQHGEIDHSGSLPELMREIPDVPI
                     YCTQNAVKSLKGHYHQDWNFVPVKTGDTLDLGESKLVFVEAPMLHWPDSMFTYMTGEN
                     ILFSNDAFGQHYATESLYNDRSSECEIYEEAIKYYANILTPFSRQVLKKIDEFLAMNL
                     PVNMIATSHGVIWRHDPLQIVHKYKEWASDYQENQITIVYDTMWNGTKAIAEAIAAGI
                     QLGDPDVTVKLYHAGKEDKNDIITQIFKAKAVLVG"
BASE COUNT         1078 a          917 c          951 g          797 t
ORIGIN      
        1 cggcagcaat gtgtggcttc aaagtgattg aagtcaagag caaagatggg atggtcgata
       61 ttgaagagtt gagacagaag gtatcagaca aaaccgcagc tctgatgatc accaacccca
      121 atacgctggg cctttttgaa aagaacatct gcgaaataga aaagattgtg cacgatgcag
      181 gcggtctgat ctactatgac ggagccaatc tcaatgccct tatgggcatc gtacgtcctg
      241 gcgacatggg agtagatgtg atgcacttca atctgcacaa gaccttctcc acgcctcacg
      301 gcggtggcgg tccgggttcc ggacctgtcg gggtcaaaaa gcacctcgaa ccttatctgc
      361 caagaccagt tgtggtcaag ggagaagatg gatttaagct ggatttcgat agaccaaagt
      421 ccatcggcaa agttgccagt ttctacggga acttcggcgt ggtactccga gccttcgtct
      481 acatgacggt gctgggtaaa tgcggtttgc ggaaggcatc tgagcttgca gtgcttaacg
      541 caaactacct gaaagcgcta ctcgaagacg ttctggacgt gccgtacagc gaaggcacaa
      601 tgcacgagtt tgtggcaacc agtaaggaca cggacctaac ggcactagag atcgccaagt
      661 ctcttctcga ttacggctac cacgcgccaa cggtctactt cccgctgatc gtgcatgaag
      721 ccttaatgat cgaacctacc gaaaccgaaa gtccagagac tttggaggag tttgccgaaa
      781 cccttaagga gattctaaag aatccagaaa gaagcaagag cgctccgcac ctcatgccga
      841 tcaagagagc cgatgaagtg aaagcggcac ggcacccggt gcttaccaga tcgcaaatag
      901 cagaataaat taggctgccc aaagaggcag cctttacagt agtggtgaag tctatgaagt
      961 tctacgaacc gaaaactata tctgtaagcc ttacaggaaa aaactgtggt ctcaactgca
     1021 agtactgcca gggccattat ctccagcaca tggtaagtgc caaagagctg aaggtcgaag
     1081 aagacgcatc ctacctcatc tccggcggtt tcgacagagc ggggaggcta cccctctacg
     1141 agaaccgcga cctactgcga aacctctgcc agaaggccaa actcaacgtg catccaggct
     1201 tcctaaagga cgaggatcta gagctcttaa aagagataga cccggtagtt tctttcgatt
     1261 tcattcaaga tcccaaagtg attcgagagg tcatcaacct cccgtacgga cctgaagact
     1321 atgtaaatca gtacaagatc atggtggatg cagggctaaa ggttgtcccc cacatgcttt
     1381 tgggtcttgg agagaaagac tccgagaccc tccaggcctt ggcagagctc cagcctgaaa
     1441 aagctgtagt cttggtgttc aggcctacaa gaggaacgtc ccttgcgggc aaagagctac
     1501 ctagcttgag ggagctcggt gcgattttgg acgatttccg caaagcctac caaggcgagc
     1561 tgatcctagg ctgcatgagg ccgggaggca cctaccgtat agagctcgat aacctagcag
     1621 cagatctggg ttttgacggc atagtgaagc ccgtaaagcg cgttaaagac ctgcttaaag
     1681 atgcagagat cttcgatgaa tgctgcgctt tttagaggag tgagaacgtg agagcatcaa
     1741 ttggaacagc aggggtttta ggtctgaaaa ggctcaagca gatctgcccc ccaaaaacag
     1801 cctacttact tacagacgga cagtgcagaa gaaactgcaa gttctgcgga aactccaatc
     1861 agcttgcaag agtaacctgg cctcatttcg ctaaagagga gatcatttcg tccttaaaga
     1921 gcacagacat gaaaaggatc tgcatccaga ccgtcgacag caaagaagct ctggaagagg
     1981 ttcgagaagt acttccagag atcatcaaaa ccggcattcc ggtctgcctt tcatcccagt
     2041 tcggcgagga gtttctcgag atgggactgg atcactggac cataccgctg gatgtggtgc
     2101 gacccgatca gtacactgcc attaagggcg ggagctttga aaaagcgctg gaccgcatca
     2161 aagccatggc cgcaaagtac ccgaaccgca tcggtacgca tctcattgcc ggactaggcg
     2221 agactgaaga agagatgata acgctcctcc acgaccttta caggtctggg gtgagtgtag
     2281 gtcttttcgc cttcactccc gtgcccggta ctgagctaga aaaccatccc aaaattcctc
     2341 tggactccta ccggaggatt caaatcggca attacttagt ctctaagtac ccagaagcca
     2401 tagaagaaat ggtctttcaa gaaggaagac tcgtcttcgc tcccttagac aaagtggaaa
     2461 gccgtgcctt tgaaacctgg ggctgcgaag actgcaacag gccgttctac aatgaatctc
     2521 ccggtggccc ttggtacaac tatccaagac ctctcagccc agaggagttc agagaagccc
     2581 tcgcagagag tggcctgaaa tagcaacacc gacctttgaa agcaggtgtg caagccttca
     2641 cgaaagcatc tttccatgcc agacagaagt ttttgttgtt tcgtttatgc aaaacctggt
     2701 aattcaacta atatggctta ttatgccaag ctcggggtga aacaggcata atacaagaag
     2761 gcgagttaag ttaaccgaaa gctaaaagaa gaagaaaagg ggatgtttac gtgaagacaa
     2821 gtaaaaagct tactgaaaaa gttacttggg ttggtaaagt agactgggaa ctgagctctt
     2881 tccatggtga agagctgtct acacatagag ggtcttctta caacgcgtat ttgatacgcg
     2941 acgaaaaagt ggccctgatc gatacggtct gggtcccttt tgacaaagag tttgttaaga
     3001 acctcaagtc ggagatcgat cttaaagaga tagactacgt aatcattcag cacggcgaga
     3061 tcgaccacag cggctcgctc cctgagctga tgagggagat tcccgatgta ccgatctact
     3121 gcacacaaaa cgctgtaaaa tccctcaaag gccactacca ccaagactgg aacttcgttc
     3181 cggtaaagac aggagatact ctggatctgg gcgagagcaa actggtcttt gtggaagcgc
     3241 cgatgctcca ctggccagat tccatgttca cctacatgac tggtgaaaac attctcttca
     3301 gcaacgacgc cttcggtcag cactatgcaa ccgaatccct ctacaacgat cggagcagtg
     3361 agtgcgagat ctatgaagag gccatcaagt actacgccaa cattctcaca ccgtttagca
     3421 gacaggtctt aaagaaaatc gacgaatttc tcgctatgaa tctgccggta aatatgatcg
     3481 ccacaagcca cggagtaatc tggaggcatg atcctctgca gatcgtccac aagtacaaag
     3541 agtgggcaag cgactaccaa gagaaccaaa tcaccatcgt ctacgacacc atgtggaacg
     3601 gtacaaaggc tatcgctgaa gccattgcag ctgggatcca gctaggcgat cctgacgtga
     3661 cggtcaagct ctaccatgca ggtaaagaag acaagaacga catcattacg cagatcttta
     3721 aagccaaagc ggtcttggtt ggc
//
LOCUS       DAOSDA010000134         3740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15595_length_3740_cov_3.422524, whole genome shotgun sequence.
ACCESSION   DAOSDA010000134 DAOSDA010000000
VERSION     DAOSDA010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15595_length_3740_cov_3.422524"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLW82_04100"
     CDS             complement(<1..513)
                     /locus_tag="PLW82_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020061932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46489.1"
                     /translation="MITITDLTKEYGPSKGVFDLSFTVNRGEVFGYLGPNGAGKTTTI
                     RNLLGFIHPDKGSCTIDGYDCHREGAKIQENLGYLPGEIAFFDAMTGDEFLHLMTEMR
                     RMKDTTKQKQLIELFDLDTKSSIRKMSKGTKQKLGLVCAFMHDPDVLILDEPTSGLDP
                     LMQNLFVNLIL"
     gene            746..1603
                     /locus_tag="PLW82_04105"
     CDS             746..1603
                     /locus_tag="PLW82_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46490.1"
                     /translation="MDRLKKPILALVLVLQGAVFVLASTSTYTYSFHYGFVSFQLSHA
                     WEVLENDSLVILRDPALDQELWLMFAPKNNSKTPKEHALAIASAFQILNPTFLVGEWL
                     SALGGKGVLFELEYTEGNASYRGIGLLYLDSTLDRSLWLHFLTPEKTFQLNEAVVFLQ
                     SFLSSLTIKTVQNNSVLRALVMDKLNYKPAQVALCARALVELFDFALDLSLSRDQKEA
                     LEADVISSWSSLSAAELALYSEYPKYLETIKRSKNPDELDALKRVLRSCFETKFRRWL
                     LQFQDEAVR"
     gene            complement(1658..>2354)
                     /locus_tag="PLW82_04110"
     CDS             complement(1658..>2354)
                     /locus_tag="PLW82_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46491.1"
                     /translation="SSSVFAKTPIPSGYSNLEMYYRHKDIDRYLFQDHKKDLEVRGIT
                     VTHTGPVKDRIEIGIMPYTEENAQFLYKALGREGIVVVPSTQATLYVKDANETVEAFA
                     PQSGTISEDVLTKYQEIALYVAENGKDLAKRGTRVTYVKPTASSVEVGILPYTEENAA
                     YLRDLFGGELEITAGAKLNHVRFGSPKPPTIQVLQNSERAFYIKLGLAVLAGGLALGL
                     LFFKPKKQHNSSL"
     assembly_gap    2355..2364
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2425..3117)
                     /locus_tag="PLW82_04115"
     CDS             complement(2425..3117)
                     /locus_tag="PLW82_04115"
                     /inference="COORDINATES: protein motif:HMM:NF025643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HOJ46492.1"
                     /translation="MKDFRRIADESMANVNVRAELKEKTLVKLYRKPNTVRFLVPALA
                     AASIMLILGIAFWPESPQSMPEDGSTEMGIMQLKTLSSPLGAPETSRFMAMDVNSWSF
                     VSVEEARDAFGEGFLTPGNIPLGFELESITGTGTEDQVENVTLTYSESASSVDGLIRF
                     ITITQEKVASSWEYYGFEEVDLLGYTGYVSASEETAELHFFLGSVRYDVIGQLQKDQA
                     IAIAKSMIQSTR"
     gene            complement(3104..3673)
                     /locus_tag="PLW82_04120"
     CDS             complement(3104..3673)
                     /locus_tag="PLW82_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ46493.1"
                     /translation="MFFLQTTNSSKAHLEKEINRLIDLYGQDVLRTAYMYLKDLQKAE
                     DVFQEVFIRVFRKYETFQGRSSEKSWIIRITINLCKDVLRSAWLKRALLTDKPVEEEL
                     PQEGESLESRVAQDAEDKLLFSEVMNLKTEFKDVILLYYYHGYDTKEISEILKVKEGT
                     VRSRLHRARQILKERLEGRVDFGDADERF"
BASE COUNT          855 a          945 c          885 g         1045 t
ORIGIN      
        1 cagaatcagg ttcacaaaga ggttctgcat caacggatcc aggccgcttg ttggctcgtc
       61 taggatgaga acgtccgggt cgtgcatgaa ggcgcagacc agccccagct tctgttttgt
      121 gcctttggac atctttctga tgctgctttt ggtgtccagg tcgaaaagct caattagctg
      181 tttctgcttg gttgtatcct tcattctccg catttccgtc ataagatgca ggaactcgtc
      241 tccggtcatg gcatcgaaaa aagctatctc gccgggaaga tagccgaggt tctcctggat
      301 tttggcccct tccctgtggc agtcatagcc gtcaatggtg caggaacctt tgtcggggtg
      361 aataaagccc aggaggttcc taatggttgt ggttttccct gcgccgttgg gcccgagata
      421 gccaaacacc tctccccgat ttaccgtgaa ggaaaggtca aaaacgcctt tacttggtcc
      481 gtactcttta gtgagatcgg taattgtgat catcgcttta gctacagaag ctgcagccca
      541 tcctcccttc ctgtgtgaac ttgtgcaccg ctatgggaat atggttaggg ttgaaaacat
      601 gtcactgcaa cactatgttt tttcccggac gacaaaaata aatcgtaata taagttgaac
      661 ataatgtagt caattcctcc atatattatt acaagaagca ccaattaaag aactttttgc
      721 caaaggcttt tgtggaggaa tgcaaatgga caggctgaaa aaacccatct tagctttagt
      781 actggtgctc cagggagcag tctttgtcct ggccagtact tctacgtata cctactcctt
      841 ccactatggt tttgtttcat tccagttatc gcatgcgtgg gaggttctgg aaaacgactc
      901 cctggtcatt cttagagacc ccgccctgga ccaggaactt tggctaatgt ttgctcccaa
      961 aaacaactcc aagactccaa aggagcatgc cctagccatc gctagcgcat ttcagattct
     1021 taaccccacg tttcttgtgg gagagtggct gtctgccttg ggcggaaaag gcgtcctctt
     1081 tgagcttgag tacaccgaag gcaatgcttc gtacaggggt atcggactgc tctatttaga
     1141 cagcaccctt gaccgttccc tgtggcttca cttcctcact cccgagaaaa ccttccagct
     1201 aaacgaggct gtagtgtttc tgcagtcgtt cctcagttct ttgaccatca aaaccgtcca
     1261 gaataattca gttctcagag cgcttgtcat ggacaagctg aactacaagc cggcccaggt
     1321 cgcgctgtgc gcaagagcct tagtggagct cttcgatttt gcactggact tgtccctatc
     1381 tagagatcaa aaagaagcgt tagaagccga tgtaatcagc tcgtggagca gcttatcagc
     1441 ggccgaactt gctctgtaca gcgagtatcc gaagtacctg gagaccatca agcgaagcaa
     1501 aaaccccgac gagctagacg ctctaaagag agttttgcgg agctgctttg agacgaagtt
     1561 ccgccgctgg ctgctgcagt ttcaagatga agcggtgcgt tagaccagtc atacatgtat
     1621 aaacaaaggc agacctgttc ggtctgccct gcgaaaacta aagggaggag ttatgctgtt
     1681 ttttcggctt gaagaacaac aaacctaagg ccagtccgcc tgcgagaact gcgagaccga
     1741 gcttgatgta gaaggctctc tctgagttct gcagcacttg gattgtcgga ggtttggggc
     1801 tgccaaacct cacgtgattg agcttggctc cggcggtaat ctccaattcg cctccaaaca
     1861 gatctctaag ataggcagcg ttttcttcgg tgtaaggtaa gatgcctact tcaacgctgc
     1921 tagctgtagg ctttacgtag gttacccgtg tgccccgttt ggcaaggtct ttgccgtttt
     1981 ccgccacata cagagctatc tcttggtact ttgttagaac gtcttcggag atggttccag
     2041 actggggagc aaaagcttca accgtctcgt ttgcgtcttt tacatagagg gtcgcctgcg
     2101 tgctaggcac gactactatt ccttccctcc ccaacgcttt gtaaaggaac tgggcgtttt
     2161 cctcggtata tggcatgatg ccgatttcaa tacggtcttt caccggacct gtgtgagtga
     2221 cggtaatccc ccgcacttcc aggtcctttt tgtgatcctg aaacaagtag cgatctatgt
     2281 ctttgtgtct gtagtacatc tccaggttac tatacccgga aggtatggga gtctttgcaa
     2341 aaacggaaga acttnnnnnn nnnnacacta agagtacaga cacaaactgc agcttcttaa
     2401 aagctctgag ttgcttcaac cagttcacct cgtgctttga atcatgcttt tggcaatggc
     2461 aattgcctgg tctttttgga gctgtccgat gacgtcgtag cggacgctgc caaggaaaaa
     2521 gtgcagttct gctgtttcct cactggcgct aacatatccg gtgtacccca acagatccac
     2581 ttcttcgaaa ccgtagtact cccaagatga cgctactttt tcttgggtaa tcgtgatgaa
     2641 gcggataagc ccatctacgc ttgatgccga ttcgctgtag gtcagagtaa cgttttctac
     2701 ctggtcttcg gttccggttc ctgtaatcga ctccagttca aaaccaagcg ggatgttacc
     2761 tggggtaagg aacccttcgc caaatgcgtc ccgcgcttcc tccacagata caaagctcca
     2821 gctgttcaca tccattgcca tgaagcggga ggtctcgggc gcgccaagtg gcgaggacag
     2881 tgttttaagc tgcataatac ccatttcggt gctgccgtct tccggcatgc tctgcgggct
     2941 ttccggccag aaggcaatac ccagaatcag catgatagac gctgcagcta atgcgggaac
     3001 taagaaacga acggtattgg gcttgcgata aagttttacc agagtttttt ctttgagctc
     3061 tgctctcacg ttgacatttg ccatggactc atctgcaatt cgcctaaaat ctttcatctg
     3121 cgtcaccgaa atcaaccctc ccttccagcc gttctttgag aatctgcctt gctctgtgaa
     3181 gcctactcct tactgtacct tccttaactt ttaggatctc gctaatctcc tttgtgtcgt
     3241 agccgtggta gtagtaaagc agtatgacgt ctttgaactc ggtcttgagg ttcattacct
     3301 ctgaaaacaa gagtttgtcc tctgcatcct gcgccactct gctttctaga gattctcctt
     3361 cttgaggcag ttcttcttcc acaggtttgt ctgtgagtag ggcccgcttt agccaagcac
     3421 ttctcaagac gtctttgcag aggttgatcg tgatcctgat gatccaactt ttttcgctag
     3481 accgaccttg gaaggtttcg tactttctaa agaccctgat gaagacctcc tgaaaaacgt
     3541 cttctgcttt ctggagatct tttaggtaca tataggccgt cctcaagaca tcctggccat
     3601 aaaggtcgat tagtcggttt atttcttttt ccaaatgcgc tttcgatgag ttcgtcgtct
     3661 gcaaaaaaaa cattggagtt cctcctgtta gccgcgccta tttgttagac gaaccaggcg
     3721 cgttttgcgc tcccgggtag
//
LOCUS       DAOSDA010000135         3739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15613_length_3739_cov_2.974484, whole genome shotgun sequence.
ACCESSION   DAOSDA010000135 DAOSDA010000000
VERSION     DAOSDA010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15613_length_3739_cov_2.974484"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..539)
                     /locus_tag="PLW82_04125"
                     /pseudo
     CDS             complement(33..539)
                     /locus_tag="PLW82_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310846.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
     gene            complement(599..1114)
                     /locus_tag="PLW82_04130"
     CDS             complement(599..1114)
                     /locus_tag="PLW82_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ46494.1"
                     /translation="MQKGQWNVSTSVRKITVAGLLGGIAVVLGATSLGYIPTPWGLNI
                     TLMHIPVILGAVLEGPYVGMATGLIFGFSSFLTPRSPAMADPLVSVLPRLVIGITAYL
                     AYKATGREWVAAIVGTATNTIGVLGMILLRGYLPLDVVVRVAYTNGVLEIIVATLIVL
                     AIVPTLKRLRD"
     gene            complement(1172..1762)
                     /locus_tag="PLW82_04135"
     CDS             complement(1172..1762)
                     /locus_tag="PLW82_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46495.1"
                     /translation="MKSKYPYIHLARRSFKDILKDDLVYLALSIIPILLWTLGVYLGV
                     LPKNMSTGWTILLAAMLFVLTYEFAESLNRPEELQGFGYGTYSRLIWVIYIRILYIVM
                     QISFRLQLPVERIVARWSRNARALARRDTGKWMYPVDAYLRQEGYEITNDLRVTEYQN
                     MIQKALNMHFTPEDHYRLRVLLRELWPEYEPKASMK"
     gene            complement(1838..3319)
                     /locus_tag="PLW82_04140"
     CDS             complement(1838..3319)
                     /locus_tag="PLW82_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ46496.1"
                     /translation="MEKAYDSKEILAARKDLDMAAELAEKVEAEGEGLFRQSRIKLEE
                     FWVNVSYSLPATTKMAYVSAEELGKKALARDISGYLLSIKELGFNGIIVETMTEDGYT
                     LYPTRDQEQHRLLQGSDVLREVGNLAREYGLQLFLIMDVGFAAKGQLPLIASKYPDWV
                     AVNEYRDIFDDYDKVYLNMSHPDVRRYYVARAQDLAGYNISALILRLDLPRRDMEIND
                     YSYDVFSRNYFQENYSYDPLKVKDRLRVDWDEWRAEQINSLVGQISRAVTTNYPGVKV
                     GAMVIVDDLYLDELKFEKLLDWKSWIERDYIDYFLPQINTAKEYDLMLRQMSAVRDTF
                     LPYPVLTLGDDMTRIEFLQSINRLLLRGSLVFKDLDELDQFSVDLLTKILTKKESTTL
                     HEDPWRALMLNVAEIETQAPASWVADIRQLSEKLSKLVMAPTGSGYIEALAHVRDHIE
                     YLKVKAITDSSAFGNRMDHELIVAKNLLELGASSRTAQGRKLY"
BASE COUNT          971 a          947 c          817 g         1004 t
ORIGIN      
        1 gacgacgtgg tgctctatcc gatgcccgcc gggggcagct tcgatgcccg gctgaacagc
       61 gcttctgccg agatgtttat cccgggacag attacgcctc cgaggtactc ccagttttcg
      121 ctaacagcac agaaagtggt tgcagttcca aaatccacga tgatcagggg cccaccgtag
      181 aggttcagcc cagctaccgc attgacaatt ctgtctgcgc caacttcctt ggggttttca
      241 tagcggatgt tcaaaccggt tttcaagccc ggtccgatgg aaagaggctc gacacctaga
      301 tactgtctac acatctctaa gatgctagga gtaagaggcg gcactacaga cgagactatt
      361 gcggcgtcta tatcttggaa cttcatatct tttgtgcgga gcagatcacc tacgagaatg
      421 cccatctcat cgctggtctt agtgatgtct gttgcaaccc gccaagatgc aatcaggtcc
      481 ttctctttgt agatgcccag taccgcatga gtgttgccaa catcaactac aagtagcatc
      541 ataagcacct tcttccccga aaatagacaa aatacgaggc aggggtcacc cctgcctgtt
      601 aatcgcgtag gcgtttaagc gttggcacga tggccagcac aataagagtt gccacaatga
      661 tctccagcac cccattggtg tatgcgactc taaccactac atccagtggc agatatccgc
      721 gaagcagaat catccccagt accccaatgg tgtttgtagc tgtaccaact atcgccgcta
      781 cccattccct acctgtagcc ttgtaggcaa ggtaggcggt aatgccgatc acaagcctcg
      841 gaaggaccga aacaagagga tcagccatag caggtgatct cggcgtaagg aaactggaga
      901 agccgaagat caaacccgtt gccattccga cataagggcc ttctagcacc gctcccaata
      961 taaccgggat atgcatgagg gtgatgttga gtccccatgg tgttggaatg tagcctagtg
     1021 aagttgcgcc gagcacgacg gcgatacctc ctagaagacc tgcaacggta atcttacgca
     1081 ctgacgtaga aacgttccat tggccttttt gcattttgaa ttaacctccc atctctgcca
     1141 aaacggtcag agtgaatttg gttctacagc ttcacttcat tgaagctttc ggttcatact
     1201 ccggccacag ttctctgagg agaaccctca gccggtagtg atcttctgga gtaaagtgca
     1261 tgttcagtgc tttttgaatc atgttctgat actcagttac tctcaaatca ttagtgatct
     1321 cgtagccttc ttgtctcaag taggcatcca caggatacat ccatttgcca gtatctcttc
     1381 ttgccagagc tctagcattt ctgctccacc gtgcaacaat ccgttcaacg gggagctgca
     1441 gtctgaaaga gatctgcatc acaatgtaca gtatcctgat ataaataacc catataagcc
     1501 tagaataagt tccatatcca aaaccttgga gttcttccgg acgatttagg gattcggcaa
     1561 actcgtaagt gagaacaaag agcatcgccg caagcaatat cgtccagccg gtagacatat
     1621 ttttcggtaa aacgcctaaa tatacaccta aagtccaaag taagatagga ataatgctta
     1681 atgcaaggta cacgagatca tctttgagaa tatccttaaa gctccttctt gccagatgga
     1741 tgtaaggata cttagatttc atctctgttc ctacctcctt tgtctatacg actcactgct
     1801 attttaccac aaactttttg ccagttatag gggagaccta gtacaacttt ctcccctgcg
     1861 cagtacggct acttgctccc aattcgagaa ggtttttcgc cacaattagc tcgtgatcca
     1921 ttctattgcc aaaggcagat gagtcggtaa tagctttgac cttgagatac tcaatgtggt
     1981 cccgaacatg ggctaaagct tcgatgtagc ctgagcctgt aggtgccata accagcttag
     2041 acagcttctc ggaaagctgc ctgatatcgg cgacccaact tgcaggtgcc tgagtctcaa
     2101 tctcagccac gtttagcatc aaagcccgcc acggatcttc gtggagcgta gttgactctt
     2161 tctttgtcag gatctttgtc aaaagatcta cagagaactg atccagctca tccagatctt
     2221 tgaaaactag gcttcctcta agaagcagtc tgttgatact ttgaagaaac tcaattcgcg
     2281 tcatatcatc gccgagagtg agcaccggat aaggcaggaa tgtgtcccgg acagcactca
     2341 tctgtctgag catgagatcg tactcttttg cagtattgat ctgagggaga aagtaatcta
     2401 tataatccct ctctatccag cttttccaat cgagaagttt ttcaaacttc agttcgtcca
     2461 agtacagatc gtcgacaata accattgcac cgacttttac ccctgggtaa ttagtcgtta
     2521 ctgctctgga gatctgcccc accaaactgt tgatctgttc tgctctccac tcatcccagt
     2581 ccactcgcag cctatccttg accttgagtg gatcgtagct gtaattctcc tggaaatagt
     2641 tgcgagagaa gacgtcgtag gagtagtcgt taatctccat gtcccttcga gggagatcca
     2701 gcctaaggat cagagccgag atgttgtagc ctgcaagatc ctgggctctg gcaacgtagt
     2761 accttcgcac gtcaggatgg gacatgttca aataaacttt gtcgtagtcg tcgaagatat
     2821 ccctgtactc atttaccgca acccaatcgg gatacttcga agcgatcaat ggcagctgtc
     2881 ctttggctgc aaagccaaca tccataatca gaaagagctg gagtccgtac tccctggcaa
     2941 ggttacccac ttccctgagc acgtctgacc cttggagaag ccgatgctgc tcctggtctc
     3001 tagttggata gagtgtatat ccgtcttctg tcatagtttc aacaataatt ccgttgaaac
     3061 ccagctcctt gatacttaat aaatacccac taatatccct agctaaagct tttttgccaa
     3121 gctcctcggc actgacataa gccattttgg tagtggcagg aaggctatag ctgacattta
     3181 cccaaaactc ttccagcttg attcgggact gtcgaaacag accctcaccc tctgcttcca
     3241 ccttctccgc aagctctgca gccatgtcca ggtcttttct ggcagcaaga atctctttac
     3301 tatcataggc tttttccata taattgaaat attgttgtgc ttcctttaaa tccttctgca
     3361 aagagctgta gagcatgggt agatctgctc tcacacctac agcaactagc aggatcagaa
     3421 ataagagcac tgtcattcgt tttcgagtca cagcttctcg cctctcattt ttgtaagatc
     3481 tatagtaaaa tcgcattgta gcatttcttg ttggacggct ttaattccta cctaaaacta
     3541 aaactgcagg cagaaatatg gtagaatagt gcttgtaaag ttgtgtaaat cacaatggag
     3601 gttccactgc aaaacgattt gcggtttcct cctggagcat ctttatggag gtgtcgcctg
     3661 tggctatgaa tagtgaaaac gagagcgcct ttctgagcga attggataga gagcagctct
     3721 taaacgatat tgagatctt
//
LOCUS       DAOSDA010000136         3737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15623_length_3737_cov_3.322379, whole genome shotgun sequence.
ACCESSION   DAOSDA010000136 DAOSDA010000000
VERSION     DAOSDA010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15623_length_3737_cov_3.322379"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLW82_04145"
     CDS             <1..520
                     /locus_tag="PLW82_04145"
                     /inference="COORDINATES: protein motif:HMM:NF021593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HOJ46497.1"
                     /translation="QSEIEYVGCSLDNPVTVVYPDVKMARIPQNLTVYARILRAHDHT
                     WQEPPARERKRSEKRRATGKGEETGVEPLFAGRTVLVVGGETMKEQLRSIVESMKGEF
                     LFQPGFDCKGTVEGNVKKADFVVCVTQDLSHSVQKLVLKAADKHQVPYFYYNDRGAAR
                     FKEFLGDLNLPN"
     gene            534..1493
                     /locus_tag="PLW82_04150"
     CDS             534..1493
                     /locus_tag="PLW82_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOJ46498.1"
                     /translation="MKYRIGIDGGGTKTLGVLITENGDILGYKEAGPSNFLSVGLDET
                     VKNLETVIYGLCMDQGLSLKDVKIGMGLAGVGREKDKETLKEALQSRNFDFVVDTDVV
                     AALMAATEGGDGVVVIGGTGSIAYGIRGGKTVRVGGWGYLLGDEGSGYYIGKRAMIEA
                     CRSYDGRSEKSLLHEAVLDYFACENHDDFISFIYELPPIRHQVADLTRKVADLANRKQ
                     DPLAITIMKDAAKELALLAVTAAKRLEFGDEEFTLGYFGGVFKAGDYIVKTFKDEVKT
                     AFTKARIEPCLHNPAIGAALMIGPLSEEALLNLRKNQKAGEES"
     gene            1490..2389
                     /gene="murQ"
                     /locus_tag="PLW82_04155"
     CDS             1490..2389
                     /gene="murQ"
                     /locus_tag="PLW82_04155"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015698385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOJ46499.1"
                     /translation="MKENLGALVTETQNPKTLQLDTLNSLEIVTLMNDEDKNVALAVE
                     KELPNIAKAVDLIVASFKKGGRLFYVGAGTSGRLGVLDASECPPTFGVPYEMVVPIMA
                     GGEGAFIKASEDVEDKPEVGRRDLLAHDVTDKDTVVGIAASGRTPYVIGALEAAKEKG
                     ASTVAVVCNPNSPIANAADVKIAPVVGPEVLTGSTRLKAGSAQKMVLNMLSTASMVQI
                     GKAYTNLMVDVQMTNSKLVDRAIRIVQIATGASTEEVKKALDESGSCKVAIVMLLAGV
                     DVEEAQRRLKEADGFVRKAIAMK"
     gene            2622..>3737
                     /locus_tag="PLW82_04160"
     CDS             2622..>3737
                     /locus_tag="PLW82_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46500.1"
                     /translation="MRKALIFLCFAFALGASVTATGTKIEIWLLGWTNEMGRIAQELV
                     DERFTPETGIIVDVKPLAYADAGNKVLLALASRDTPDIISCGTDVADLALRGGLIDLA
                     AFKPDEYAELEQKLFSSVMGPFTFEARRFALPSDVSCLVAAYRTDILADLGMEIPQTW
                     EEVTSAQPKALARGKTFAFMRFDEMWAFYCMITQYGGNFFSSDGFSSALDRPESVEAF
                     KAYVELFTKHGFPKEVVGVTPFIEGEQILYVDGLWLYPTIETVAPQLKGKWQVSLLPG
                     VERDGKLYNGSSPGSVLLGISTYSKHKEEAWEFLKWFLGTDVLTEVANQVLAKGSGYM
                     WLPANKVAMQNVNLPEDVKKVYFDQIEACIPIPYGVNA"
BASE COUNT          985 a          854 c         1028 g          870 t
ORIGIN      
        1 ccaaagcgag attgaatatg taggctgcag tcttgataat ccggtaacag tggtctatcc
       61 tgatgttaaa atggcaagga tcccccagaa ccttacggtc tacgcccgaa ttctcagagc
      121 tcacgatcac acctggcagg aacctccggc tagggagaga aaacgcagtg agaagcggag
      181 agccactggt aagggtgaag agacaggtgt cgagccgctc tttgccggcc gcacggtttt
      241 ggtagttggg ggagaaacca tgaaggaaca gcttaggtcg attgtggaaa gtatgaaggg
      301 cgagtttctt tttcagcccg gctttgactg taagggaacg gtggaaggca atgtgaaaaa
      361 ggctgatttt gtggtctgcg tgacgcagga cctttcccac agtgtgcaga aactggtcct
      421 caaagccgca gacaagcacc aagttcctta cttttactac aacgacaggg gagcggcgcg
      481 gtttaaagag tttcttggcg atctgaatct gccaaactag aggtgatagt gtcatgaagt
      541 accgcattgg tatagatgga ggcggcacga aaacgctggg tgtcttaatc actgaaaacg
      601 gagacattct cggctacaaa gaagcaggcc cgagcaactt cttgtctgta ggtttggacg
      661 aaacggtgaa gaacctggag actgtaatct acggactctg catggaccaa ggtctaagtc
      721 tcaaagacgt gaagatcggc atggggcttg cgggcgtagg tagagaaaag gataaggaga
      781 cgttgaaaga agctcttcaa agtcggaact tcgattttgt tgtagacacc gacgtggttg
      841 ctgcgctgat ggctgcaact gaaggcggag acggtgtcgt ggttatcgga ggaactggct
      901 ccattgccta cggcattcgt ggtgggaaga ccgtaagagt gggaggttgg ggctaccttc
      961 tcggcgatga aggttccggt tactacatcg gaaaaagagc gatgattgag gcctgtagaa
     1021 gctacgacgg ccgttccgaa aagtccctgc tccacgaagc agtccttgac tacttcgcct
     1081 gcgaaaacca cgatgatttc atttctttta tctacgaact gccgccaatc aggcatcagg
     1141 tagcagacct caccaggaag gttgcagatc ttgccaacag aaagcaggac ccgcttgcta
     1201 taaccatcat gaaggatgcg gcaaaggagc ttgccctcct ggcagttacc gctgcaaaga
     1261 ggcttgagtt cggcgacgaa gagttcacct tggggtactt cggcggagtg tttaaggccg
     1321 gagactatat tgtcaaaacc ttcaaggatg aggtaaaaac cgcttttacc aaagctcgga
     1381 tcgagccctg tttgcacaac cctgcaatcg gagctgccct gatgatcgga cctctaagtg
     1441 aagaagcttt gctcaacttg cgaaagaacc aaaaggctgg tgaagaatca tgaaagaaaa
     1501 cttaggtgca ctggttaccg aaacgcaaaa ccccaaaacc ttgcaactag atacgttgaa
     1561 ctcattggag atcgttactc tgatgaacga tgaagacaaa aacgtggctt tggcggtcga
     1621 aaaggaactg ccaaatattg ccaaagcagt ggatctcatt gtggcatctt tcaagaaggg
     1681 cggacgcctc ttctacgttg gagccggaac cagcggcagg ctgggagtgc tggatgccag
     1741 tgagtgtcca cccacttttg gcgtgcctta tgagatggta gtgcctatta tggcaggggg
     1801 cgagggtgcg tttatcaagg cctctgaaga tgtagaagat aaacccgaag tcggaagacg
     1861 cgatttgctt gcccacgatg ttaccgataa agacactgta gtgggcattg ccgctagcgg
     1921 gcgcacgccc tatgtgatag gcgctctgga agctgcaaaa gaaaaaggcg caagtaccgt
     1981 tgctgtggtc tgcaatccca actcgccgat cgccaatgcc gccgatgtga agattgctcc
     2041 ggtggtcggt ccggaagttc ttaccggctc caccaggctg aaggcaggct ctgctcagaa
     2101 gatggttctg aacatgcttt caactgcttc aatggtacag atcggcaaag cttacaccaa
     2161 cttgatggtg gatgttcaga tgaccaactc caagctggta gacagggcca tccgcattgt
     2221 gcagattgca accggtgcgt ccacagaaga agtgaaaaag gctcttgacg agtccggctc
     2281 ctgcaaagtg gccattgtga tgctgcttgc tggagtagat gttgaagaag cccaaaggcg
     2341 tttgaaagag gccgatggtt ttgtgagaaa ggccattgca atgaaataga tttgacgaaa
     2401 gcagcagccc tctggctgct gctttcgcac tgtactaata cagatatgca aaaaccacta
     2461 catattggca attcttctag agaacccaat tttatgttag aaaatgcatt ggttttcaat
     2521 acttaaaata ctgcaaaaac tacagggatc atcatctgaa tcgagaataa tgacttaaat
     2581 gaaataggtg gtgtgcccag acacaggaga ggagggttca tgtgcgtaaa gcattgattt
     2641 tcctatgctt tgctttcgct ctcggcgcta gcgtcactgc aactggcacc aaaatagaga
     2701 tctggctcct cggctggaca aacgagatgg ggagaattgc tcaggaactt gtagatgagc
     2761 gcttcacccc agagaccggg atcatcgtcg acgtaaagcc tttggcttat gctgatgccg
     2821 gtaacaaagt tcttcttgct cttgcaagca gggatacgcc agatatcatt tcttgcggta
     2881 ccgatgtagc ggatctagca cttagaggtg gtctcatcga tcttgctgcg ttcaaacccg
     2941 acgagtacgc cgaactggaa cagaagctgt tttcgtcggt aatgggtccg tttacgttcg
     3001 aagcacgccg ttttgccctg cccagcgatg tctcctgtct agtggcagcg taccgcacag
     3061 acattttggc agatctgggt atggagatac cccaaacatg ggaagaagtt acctctgccc
     3121 aacccaaggc tttggcaaga ggtaagacct ttgccttcat gaggttcgac gagatgtggg
     3181 ccttctactg tatgatcact cagtacggcg gtaacttctt ctcaagcgat ggattctcca
     3241 gtgcattaga taggcccgag tcggtagaag cttttaaggc ttacgtagag cttttcacta
     3301 aacacggctt tcctaaagaa gtggttggag tcacaccttt catcgaaggt gagcagattc
     3361 tatacgtgga cggtctgtgg ttgtacccga ctatagaaac tgttgcacct cagctcaaag
     3421 gcaaatggca ggttagcctt ctccctggag tggaaagaga cggcaagctg tacaacggct
     3481 cgtcgccggg aagcgtactt ctcggtattt ccacctactc caaacataaa gaggaagcct
     3541 gggagttctt gaaatggttc cttgggaccg atgtcttaac cgaggtcgcc aatcaagtac
     3601 tggccaaagg ttccggttac atgtggcttc cggccaacaa ggttgctatg cagaacgtta
     3661 accttcccga agacgtcaag aaggtttact tcgatcaaat cgaagcctgc ataccaatcc
     3721 cctacggggt gaatgcc
//
LOCUS       DAOSDA010000137         3726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15686_length_3726_cov_2.807137, whole genome shotgun sequence.
ACCESSION   DAOSDA010000137 DAOSDA010000000
VERSION     DAOSDA010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15686_length_3726_cov_2.807137"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1983
                     /locus_tag="PLW82_04165"
     CDS             <1..1983
                     /locus_tag="PLW82_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOJ46501.1"
                     /translation="EPIRKSTDTDEHFKFVYSRNEDGYFVQAGMYAEHVYQMTEDYSL
                     QNIVERTVDNESIYYAFILDENLVAIADSQPQYAGVSYADNEDYQMALRGTPKAKTWF
                     REGLHGPVLEVAVPFYNNGDIEGVLAVGISQEELNLILRTSAVGFLSTAIVLSLAFFW
                     VQQINIIKPIGLMDRQLRYLDFNLERRQKLNFASNDPFAGLYCSINSLLERIYVHFEK
                     NEQLRKMIEALATKDPLTGLPNRRVFGEHLNAHLEREEPVAVLMLDIDNFKDVNDTFG
                     HVYGDQVLIEIGKLLLKQVNENVGVYRFGGDEFLLLVRGAQDADAVISFVENLISIFN
                     DPIRIAGDELYVQCSIGIAVYPEDAHTETDLVMFADLAMYSAKLVAKSSYVFYHPSMG
                     ESLKVKTTIEKEIRRALEEDGFTILYQPKVASEDGTISGYEALLRVQGLDYPILQVIE
                     VAEERGFIVEIGRWVIREVIHQMKRWKDKGLPPKSVAINLSARQLYDEQFIPLLQQLL
                     DETGIQPSSLEIEITENLLINQEETTLAFLKKLKEMGITISMDDFGKAYSSLNYLTFL
                     PVDVLKLDRSIANKYSDEKTKSVIKGIITIARDLNLKVVAEGVETVEQYLFFREIGCD
                     YIQGYVFSRPLCVEAAEEIYNEKLEAKSDVSEEVES"
     gene            2076..2555
                     /locus_tag="PLW82_04170"
     CDS             2076..2555
                     /locus_tag="PLW82_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ46502.1"
                     /translation="MKQLLDSDGEFDTMLEIQRTKEEEIHIVAGWADDQSIENWIGVS
                     DWHEYWSTLKANPDWYFFSVFSDGDLVGLVWGERDNEGVLTIALIVRPDLQRQGLGQS
                     TLRLLVQNVREILPEGIRSIEAHIFPQNAVSIRCFAGAGFQFAGEGEDGDLVYRYDL"
     gene            complement(2655..>3726)
                     /locus_tag="PLW82_04175"
     CDS             complement(2655..>3726)
                     /locus_tag="PLW82_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ46503.1"
                     /translation="GKAEGTYGSFWTYRRLIDKNNFDDPAYPNDLAMINWPGNDFIGG
                     SFIDVSEEEKERLFKAAKDLALGFLYWLQTEVKRDDGGYGYPEFKLRKDVMGSEDGLS
                     KYPYIRESRRIKGMTTVIEQDVSVEFLEGPRARFFRDGAGLGKYFIDIHSSGYEETAK
                     TSQVYPFQVPLGALIPRRVKNLLAGGKNLAVTHITNGTYRLHPVEWAVGEAAGILAAT
                     ALFHDCVPQQVWLDERLLRKTQANLIDHGAPVFWYVDVPIGHPIFKALQFTAAFGTYQ
                     YDKDKNLMFYPDNEITQSDLELWAQGLKDGAGIPANPESAEAFLTSIAERFDVEAPVL
                     ADNTRKSFAVALDEILRDAYLR"
BASE COUNT          958 a          872 c          988 g          908 t
ORIGIN      
        1 gagcctatcc gcaagtcaac cgataccgat gaacacttta agtttgtcta tagccgcaac
       61 gaagacggct actttgtgca ggcgggcatg tacgcagaac atgtgtacca aatgaccgaa
      121 gattacagtt tgcagaatat cgttgaaaga acagtcgaca acgagagcat ttattatgcg
      181 tttatactgg atgaaaacct cgtcgctatt gccgactccc agccgcagta cgcgggtgta
      241 agctatgcag acaatgaaga ctaccagatg gcgctgcgtg gtacgcccaa agctaagacc
      301 tggtttaggg aaggtcttca cggtccggtt ttagaggttg cagtgccgtt ttacaataac
      361 ggggatatcg aaggcgttct ggcagtaggg atcagccagg aggagctcaa cctcattttg
      421 cgtaccagtg cagttgggtt tctcagtaca gccatcgtcc tcagcctggc gtttttctgg
      481 gtgcagcaga tcaacattat caagcctatc ggtcttatgg acaggcagtt gagatacctc
      541 gacttcaact tggagagacg acagaagttg aattttgcaa gtaatgatcc ctttgccggt
      601 ctgtactgca gcattaacag ccttctcgag cgtatctacg tccattttga aaagaacgag
      661 cagctgcgta agatgatcga agccctagcc accaaagacc ctctgacggg acttcccaac
      721 cgcagggtgt ttggcgagca tctcaacgcc catcttgaga gagaagaacc ggtggcagtg
      781 ctcatgctgg acatcgacaa cttcaaagac gtgaacgaca ctttcggcca cgtctacgga
      841 gatcaggtcc ttattgaaat cgggaagttg ctacttaaac aggtcaatga aaacgtgggg
      901 gtttaccgct tcggcggaga tgagttcctg cttcttgttc gcggtgcaca agacgctgac
      961 gccgtgataa gctttgtgga gaatctaatc tccatcttca acgaccccat acgtattgcc
     1021 ggtgatgaac tctacgtcca gtgcagtatc gggattgcag tatacccgga agatgcgcac
     1081 acagagaccg atctggtgat gttcgccgat ttggcgatgt acagcgcaaa gcttgtagcc
     1141 aagagcagct atgtgttcta tcatccttcg atgggcgaat cgctcaaggt gaagaccacg
     1201 atcgaaaagg agatacgcag ggctcttgaa gaagacggtt ttaccatttt ataccagcca
     1261 aaggtagctt ctgaagatgg aaccatcagt ggctacgaag cgcttctcag agtgcagggt
     1321 ttagactatc ctattctgca ggtgatcgag gtagccgagg aacgaggttt catagtagaa
     1381 ataggccgtt gggttattag agaagtcatt catcagatga agcgctggaa ggataagggg
     1441 cttccgccca agtctgtagc catcaatctt tctgccagac agctttacga cgagcagttt
     1501 attccgctct tgcaacaact tctggacgaa acaggtattc agccttcatc tctggagatt
     1561 gagattaccg aaaacctcct gatcaaccaa gaagaaacta cactggcgtt tctcaaaaag
     1621 ctcaaggaga tgggtattac catttccatg gacgacttcg gcaaagccta ctcatcattg
     1681 aactacctga cattcctacc ggtggatgtt ctcaaacttg atcggtctat cgccaacaag
     1741 tacagcgatg aaaaaaccaa gagtgttatt aaggggataa tcactatcgc gcgggacttg
     1801 aacttgaaag tggtagctga aggtgttgaa acggttgagc agtacctttt cttcagagag
     1861 ataggctgcg actatattca agggtatgtc ttcagcagac cactctgtgt tgaagctgca
     1921 gaagagatct acaacgaaaa acttgaagca aagtccgacg tctccgaaga ggtggaaagt
     1981 taaccatcac cacttcggaa ggttcgtgcg caagacgata cgtagcctcc aaaagaaaag
     2041 ctgcctccac ggggatgcag cttttctgac gatatgtgaa gcaactacta gattccgatg
     2101 gggagtttga cacaatgctg gagatacaga gaactaagga agaagagatc catattgtag
     2161 ctggatgggc agatgatcaa tcgatcgaga actggattgg cgtcagtgac tggcacgagt
     2221 actggagtac cctaaaggca aaccccgact ggtacttctt cagcgtgttc agtgacggag
     2281 accttgttgg gcttgtctgg ggagaaagag acaatgaagg tgttctgaca attgcgctga
     2341 tcgtaaggcc agatctgcag aggcaggggc tcgggcagag tactttgcgc ttactggttc
     2401 aaaacgtaag ggagattctt ccagagggga taaggagtat cgaagctcac atcttcccgc
     2461 aaaatgctgt gagtatcagg tgttttgccg gtgccggctt tcagtttgcc ggtgaaggag
     2521 aagacggcga tctggtctac aggtatgacc tgtgacactg ccggaatagg ctgatcccaa
     2581 tcgaaactaa attcaataca accaaaaggg agtgccagct gcttaaggca gaactgggca
     2641 ctcccttttc gcttttacct caaatacgcg tctctaagga tttcgtccaa agcgacggca
     2701 aatgatttgc gtgtgttgtc tgcaagaaca ggtgcttcga cgtcaaagcg ctccgcgatg
     2761 gacgtcaaga acgcttctgc gctttccggg tttgcgggaa taccggcacc gtctttaagc
     2821 ccttgagccc acaactccag atccgattgt gtgatctcgt tgtcaggata gaacatcagg
     2881 tttttgtctt tgtcgtactg gtaggtgcca aaagctgcag tgaactggag cgctttgaag
     2941 atggggtggc cgataggcac atccacatac cagaaaaccg gtgctccgtg gtcgatcagg
     3001 ttggcctgag tcttacgcag gagcctttca tccagccaaa cttgctgagg cacgcagtcg
     3061 tggaaaagcg ctgttgcagc caaaatgccg gcagcttctc ctaccgccca ttctacagga
     3121 tgcagtctgt aggtaccgtt ggtaatatgg gtcactgcca ggttctttcc accggcaagg
     3181 aggtttttca cccggcgggg aatcagtgcg ccaagtggaa cttggaaggg atagacctgg
     3241 gaggttttgg ctgtttcttc gtatccggag gagtggatgt cgatgaagta cttgccaagg
     3301 ccggctccat cgcggaagaa gcgggctcta ggaccttcaa gaaactctac gcttacatcc
     3361 tgctcaatga cagtggtcat acctttgatg cgccgagact cgcggatgta cgggtacttg
     3421 ctgagcccat cttcggaacc cattacgtct ttgcgtaact tgaactcggg atacccatat
     3481 ccgccatcgt cgcgtttgac ttcggtctgc agccagtaca gaaagcccaa ggccaggtct
     3541 tttgctgctt taaacaagcg ttccttttct tcttcggaaa cgtcgatgaa gcttccaccg
     3601 atgaaatcat ttccaggcca gttgatcatg gccaggtcgt ttggatatgc cggatcgtcg
     3661 aagttgtttt tgtctataag gcgtctgtac gtccaaaacg aaccgtaggt gccttctgct
     3721 ttcccg
//
LOCUS       DAOSDA010000138         3698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15855_length_3698_cov_2.769695, whole genome shotgun sequence.
ACCESSION   DAOSDA010000138 DAOSDA010000000
VERSION     DAOSDA010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15855_length_3698_cov_2.769695"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLW82_04180"
     CDS             complement(<1..432)
                     /locus_tag="PLW82_04180"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOJ46504.1"
                     /translation="MLLEKGVSILHEVKVRGVGIDPKTMSTVLLLSSGDKVLPMFIGL
                     AEATAIASELEGVKSPRPQTHDLMIRILTTLKYRIEKVEITDFKDNTYYAVITLVQDG
                     TGDGAPVKLEIDARPSDAVALALKSNAPIYLSEKLAGDLVLR"
     gene            complement(477..986)
                     /locus_tag="PLW82_04185"
     CDS             complement(477..986)
                     /locus_tag="PLW82_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HOJ46505.1"
                     /translation="MSGYVPALIVLFCIVLGLKARINIFEHFYEGAKDGIIALWRILP
                     TIIGIFVAMALVRNGILWGYIVDTIGLVLSPLGVSKELVPLALMRPFSGSASLAILTK
                     LLGEHGPDSLIGLSASTFMASTETAFYVAATYLASVQIKKSRHIFIVALVVQFLVLIA
                     SSLIWRVLL"
     gene            complement(987..1544)
                     /locus_tag="PLW82_04190"
     CDS             complement(987..1544)
                     /locus_tag="PLW82_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46506.1"
                     /translation="MSSVWAGLMIIGIVSAILEGKPERLLVCITQGGSDSIQYVLSLF
                     GIMAFWSGIYGILDKTGLLGFLTAKFARPIFKRVFRRPLSPAQEEAIFASIAANLLGL
                     GNAATVWGLRAMAELKKGGQLTPNMATFLVLLSTGFTVFPSTVISMRAAVGSQKAALI
                     WVPTLVVSVFGAALALLLDRLWGEG"
     gene            1759..2511
                     /locus_tag="PLW82_04195"
     CDS             1759..2511
                     /locus_tag="PLW82_04195"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ46507.1"
                     /translation="MRQKNRISLRKSLVITAVMVAVSVYVFAGSYTVRSGDTLWIIAN
                     KHGTSLETLRKLNPGVGTMIYPGQVITVPSTGSGGGSTITVQMGDTLWKIASKYGTTV
                     NSIKSLNGLTSDMIYPGQVLRVSSGGASQQTNLDIDLLARLVHAEAQGEPYEGQVAVA
                     AVVLNRMKDSRFPNTLEGVVYEKHAFEPVSNGHIYKPASSTAYKAAQDAINGWDPSYN
                     SIFFYNPDKLKGWNWVLTRPVVRRIGNHVFAR"
     gene            complement(2599..2751)
                     /locus_tag="PLW82_04200"
     CDS             complement(2599..2751)
                     /locus_tag="PLW82_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46508.1"
                     /translation="MNAVFLIAALFNVAAGIMYLTRQRNKKGAIYSFILAAVILIIGL
                     IVGKPA"
     gene            complement(2954..>3698)
                     /locus_tag="PLW82_04205"
     CDS             complement(2954..>3698)
                     /locus_tag="PLW82_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010273477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ46509.1"
                     /translation="FRLPLKESLEKAKEVGGHGVQLWIATGELAPENLTETGKKDLLH
                     LLESNNLEISATCGDIGGFANPQGLEERVERTKRLIDLSVSLNAPIMTTHIGVVPEDW
                     RETEQGKMMLDAVKEVAKYAENHGAFLASETGPESPELMKDFLDAVQSKGIAVNYDPA
                     NLVMKGFDYLGGVKVLAPYIVHTHAKDGIRVDGKGQEVALGEGHVDWDAYIAAMKEIG
                     FNGYFTIEREVGPDPVADIKKAADFLKKY"
BASE COUNT          986 a          947 c          840 g          925 t
ORIGIN      
        1 ccttaagacc agatcaccag ctagcttctc actcaagtag attggggcat tgctcttaag
       61 tgccaacgct acagcatcag aagggcgagc atcaatctct agctttacag gagcaccatc
      121 tcctgtaccg tcttgaacaa gcgtgataac tgcataatac gtattgtcct taaaatcggt
      181 gatttctact ttttcaatgc ggtattttaa ggttgttaaa atcctaatca tcaaatcatg
      241 ggtttgaggt cttgggcttt ttacaccttc tagctcggat gctatagcag ttgcttcggc
      301 aaggccgata aacatcggta aaaccttgtc cccactggat aagagtagaa cagtactcat
      361 tgtcttggga tcaataccta ctccgcgtac ctttacctca tgcaaaatgc tcaccccttt
      421 ctctaaaagc attataacaa gaacggtcaa acatgaacac tggcatttgc tttaagttat
      481 aacagaactc tccatataag gctgcttgcg ataaggacca agaactgcac aactaaagct
      541 actataaaaa tatgcctgct ttttttgatc tgtactgagg ccaggtaggt cgctgcgacg
      601 taaaacgcag tttcggtgct ggccatgaac gtggatgcgc tgagaccaat cagcgaatcg
      661 ggaccgtgtt ccccaaggag ttttgtcagg atagcaaggg atgcggagcc tgaaaacggc
      721 cgcatcaagg ccagtggcac cagctccttc gagacgccaa gtggactgag aacaaggccg
      781 atcgtatcta ctatataccc ccacagtata ccgtttctaa ccagtgccat agctacaaat
      841 atgccgataa ttgtggggag gatgcgccaa agggcaatga tgccatcttt tgcgccttcg
      901 tagaagtgct cgaaaatgtt gattctggcc ttcaatccca agacgatgca aaacaagaca
      961 atcaacgccg gcacgtaccc actcatttac ccctcacccc acagccgatc gaggagtaaa
     1021 gcaagggctg ctccaaagac agaaacgact agcgtcggca cccagatcaa cgccgctttc
     1081 tgggagccca cagccgctct catgctgatc accgttgacg gaaacaccgt aaagccagtg
     1141 ctaagaagta ccaggaacgt tgccatgttc ggtgtgagct gtccgccttt ttttaactcg
     1201 gccatggctc taagacccca gaccgttgcc gcgtttccga ggccaaggag gttggcagcg
     1261 atactggcaa agatagcctc ctcctgggca gggcttagcg gtctgcggaa aactcttttg
     1321 aatataggtc tggcaaattt tgcggtcaaa aagcccaaaa gtcccgtttt gtccaaaatc
     1381 ccatagatgc cggaccaaaa agccataata ccaaacagac tcaatacgta ctgtatggaa
     1441 tcgcttccac cctgggttat gcaaaccaaa agcctttcag gcttaccttc gagaatcgcc
     1501 gagacaatac cgataatcat caaacctgcc cacactgagc tcatcttcga ccctcctctt
     1561 acagactata tggcaggatg agggcgatta ttaacatctg aagtgtgcag gccttcttcc
     1621 tagagatttc caaagctggt taaggtgcca gaaatagtgc aatgacacca tttgtgaaag
     1681 atcttataat gtgggtaagc ctgagtgatc ggtatatcac taggcacgaa ctcacaaagt
     1741 ttggaaggag gaccatcttt gcgacaaaaa aacagaataa gcctgagaaa atcactggtg
     1801 atcactgctg taatggttgc agtatcagtc tatgtatttg ctggttcata tacagtaaga
     1861 tcgggagata cactctggat catcgccaac aagcacggaa ccagcttgga aaccttgagg
     1921 aagttgaatc caggtgtggg aaccatgatc taccccggtc aggtgatcac cgtgccttct
     1981 actggttccg gaggtggcag taccatcacg gtccaaatgg gagacaccct ctggaagatt
     2041 gcttccaaat acggcactac ggtgaattcc attaagtcgc taaacggact gacatcagat
     2101 atgatttatc caggccaagt cttaagggtc tcctctggcg gggccagcca acagaccaat
     2161 ctggacatcg atcttctggc aaggctcgtt cacgccgaag ctcagggaga accttatgaa
     2221 ggacaagttg cagttgccgc tgttgttttg aatcgcatga aggattcccg ctttcccaat
     2281 actcttgaag gcgtagtcta cgaaaaacat gcatttgagc cggtttctaa cggtcacatc
     2341 tacaagccag caagcagcac tgcctacaaa gcagcacagg acgccatcaa cggttgggat
     2401 cccagctaca acagcatctt cttctacaac cctgacaaac tcaaagggtg gaactgggtt
     2461 ctcacaaggc ccgttgtccg tagaatcggc aatcatgttt ttgcaagata aaccaataac
     2521 taaaaacctt tgacaacctg cgaaatcgcg gttgcaaaga gcctgacaaa taacactgtc
     2581 aggctctttt ctctgctttt atgcaggttt gccaacaatt agtccaatga tcaggatcac
     2641 tgctgccaaa atgaagctgt agattgcacc ctttttgttg cgttgcctgg tcaagtacat
     2701 aatgccagct gctacgttaa aaagagccgc aatgagaaaa acggcgttca tcagataacc
     2761 tccatcctcc aaatggcaca aaaccggtgt acaacttgat tacagtattc ttcatcttaa
     2821 tcccgtttcc cttgtttgca acgggctttg ttcaagcagg gcagctgcgt attagagcag
     2881 ctgcccctct ataaaaaggc tgctgcacac tttgtcaatg aagcgtgcaa cagcctctgt
     2941 agtcagttaa aacttagtat tttttcaaga agtcagctgc tttcttgata tctgccactg
     3001 gatcgggacc tacttctctt tcgatagtga agtagccgtt gaagccgatc tctttcatcg
     3061 ctgcaatgta tgcatcccag tcgacgtgtc cctcaccgag agccacttct tggcctttgc
     3121 catcaactct gatgccgtct ttagcgtgag tgtggacgat ataaggagcg agaaccttta
     3181 cgcctcccaa gtagtcgaag cccttcatga ccaggttggc gggatcgtag ttaacggcga
     3241 tacccttgga ctgcactgca tcgaggaagt ccttcataag ctccggactt tcaggaccgg
     3301 tttcgctagc caaaaacgca ccgtggtttt ctgcgtattt tgccacttct tttacagcgt
     3361 caagcatcat cttgccctgt tcggtttctc tccaatcctc gggaacaacg ccgatatgag
     3421 tggtcatgat cggagcgttc agagatactg acaggtcgat caggcgcttg gtcctttcga
     3481 cgcgctcttc tagaccttgc ggattggcaa acccgccgat atcgccgcag gttgcagaga
     3541 tttccaggtt attggactcc aaaagatgca ggagatcctt cttgccggtt tctgtgaggt
     3601 tctcaggggc aagttctcct gttgcgatcc acagctgtac accgtggccg cctacctcct
     3661 tagctttctc aagcgactct ttcagcggaa gtctaaac
//
LOCUS       DAOSDA010000139         3688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15911_length_3688_cov_3.074043, whole genome shotgun sequence.
ACCESSION   DAOSDA010000139 DAOSDA010000000
VERSION     DAOSDA010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15911_length_3688_cov_3.074043"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLW82_04210"
     CDS             complement(<1..362)
                     /locus_tag="PLW82_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46510.1"
                     /translation="MPGKHVFNYDLDLSMNRANGTVTFDESGTITSSINGEVLLTLSA
                     DNMTELSVSGGVGCGFLYATMKDESEVLICRFTMSVLKAAGEFCKVVNYYINSKEFPK
                     SEEKHKLVCEKCGRPFAEG"
     gene            complement(391..933)
                     /locus_tag="PLW82_04215"
     CDS             complement(391..933)
                     /locus_tag="PLW82_04215"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOJ46511.1"
                     /translation="MENTNEASRSLASAIDIGFLDPKKAEFYVTAGGFTGLRYEGNDY
                     KRITLRRAFPIGKPFEYISVADKDNKEIGIIRSLAGLDKDQYEIVVAELEKRYYSPTV
                     YEVKSVKDKLGYVYMELLVGKNGATYTKNCAVKDVTRNIRLLSEDTLAIFDVDGNRYI
                     VESLSKLDKKSLKRLEPYLL"
     gene            complement(963..3230)
                     /locus_tag="PLW82_04220"
     CDS             complement(963..3230)
                     /locus_tag="PLW82_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46512.1"
                     /translation="MKTLIPEYMVSPFETQGIDASKLIYAVHADSSDDSGYLDAYLAV
                     SSDSLYILLGEEKVVKASGARRIVAVYEPKELHVIPLEDVGNLSTERLVSTVRLCSEK
                     DDKTSILLVFSIGYLGAVEKLIKVISNIREGKDPLTDLPVEEELFCPKCGTRYPEPVR
                     KICPKCLDRVSITKRLFSFFGHYKTKVFWVFVTMLAGSVFSMITPLVSSRFFFDEVLT
                     EGGSYYGYILGAVLMILIVRAVNTGLNILYSYVLARTVPWIVQDLKMKIFEAMQRLDV
                     GFYTSKRTGNLMNRVNQDANNIYWFFVDGLPYVVVNLLTFTGVVLVMFLLNAKLAIIC
                     LVIIPLAILFFRVLWSIFRRFHHKNWVYHSQLNSLVSDAVNGQRVIKAFAKEDTETER
                     FSVVSGKQTLVDIHTMNTVTTAFPLIYLFMFLGQVVVTGVGGIMVLNGTEGMTIGVLM
                     SFISYLTMLYGPLEFMSWVSNWWARCVDSAQRVFEIIDAKPEVTDPENPVVLDRIRGD
                     IELKDVKFEYDAATPVLKGVNLSVKAGQMLGIVGKTGAGKSTMANLIARLYDVTEGTI
                     TIDGVNVKELSLEQLRKSVGIVSQDIYLFIGTIADNIRYARPEATMEDVIRAAKAAAA
                     HEFIMKLPDAYETRVGAGGQDLSGGEKQRLSIARTILQDPDILILDEATAAIDTETEA
                     KIQNALSQLQRGRTTLAIAHRLSTLRDADTLAVLKDGVIVESGTHRELMEKKGEYHKL
                     YTIQLEGLKIINMDA"
BASE COUNT          877 a          975 c          876 g          960 t
ORIGIN      
        1 ccctcggcaa aaggccttcc gcacttttcg cataccagct tgtgcttctc ttccgacttg
       61 ggaaactctt tggagtttat atagtagttg accaccttgc agaactcccc tgccgctttc
      121 aaaaccgaca tggtaaagcg acagatcagg acctcgcttt catctttcat ggtggcatac
      181 aggaaaccgc agccgacgcc tccggaaact gagagctcgg tcatattatc tgcagagaga
      241 gttagtagaa cctctccgtt tatactcgag gtgatcgtac cgctttcatc aaaggtgacc
      301 gtgccatttg ctctattcat tgaaagatcc aaatcgtagt taaacacgtg ttttccgggc
      361 atttggcatc acttctttct caggttctac ctagagcagg tatggttcga gcctctttag
      421 gctctttttg tccagtttgg acagagactc cacaatatac ctgttgccgt ctacgtcgaa
      481 aatcgccagc gtatcttcgc tgagaagcct gatgttgcgg gtcacgtcct ttaccgcgca
      541 gtttttggtg tacgttgcgc cgtttttccc caccagaagc tccatgtaaa cgtagcctag
      601 tttgtcttta acggacttaa cctcgtacac cgtaggactg tagtagcgct tttccagctc
      661 cgctactaca atctcgtact gatccttgtc gaggcccgca agggaacgga taataccgat
      721 ctccttgttg tccttgtcgg ctacggaaat gtattcgaaa ggcttgccga taggaaaggc
      781 cctgcgcagc gtgatgcgct tgtagtcgtt gccttcgtat ctcagacctg taaaaccgcc
      841 ggcagtaacg tagaactctg cctttttggg atctaaaaag ccaatatcta tagcagaagc
      901 taaacttctt gaagcttcgt ttgtgttttc cattacatta ccctcctacc gaacctgaac
      961 ttctaagcat ccatattgat aatttttaaa ccttccagct gaatagtgta aagcttgtgg
     1021 tactcacctt ttttctccat cagttctctg tgcgtaccgc tttccacaat caccccatcc
     1081 tttagaactg ccaaggtgtc ggcatctctg agggtggaga gcctatgggc gatggccaat
     1141 gtggttctgc ctctctgcag ctgggaaagg gcgttctgga ttttggcttc ggtttcggtg
     1201 tctatagcag cagttgcttc gtcgagaatc aggatgtcgg gatcctgcag gatggtgcgg
     1261 gcaatggaaa gacgctgttt ctctccgccc gaaagatcct gcccgcctgc gccaaccctg
     1321 gtttcgtagg cgtcgggcag cttcataatg aactcgtgag ctgctgccgc ttttgccgcc
     1381 ctgattacgt cttccattgt ggcttcaggc ctggcgtagc ggatgttgtc ggcgatggtg
     1441 ccgatgaaca ggtaaatgtc ctgggacact ataccgacgc tctttctcag ctgctcaagc
     1501 gagagctctt tgacgttaac cccgtcgatg gtaatggtac cttcggtaac gtcgtagagg
     1561 cgggcaatga ggtttgccat ggtggactta cctgcaccgg tcttgcccac aatgccgagc
     1621 atctggcctg cttttactga aaggttcacg ccttttagta ctggagttgc ggcatcgtac
     1681 tcgaacttca catcctttag ctcgatgtct cccctgatcc tgtccaggac tacagggttt
     1741 tctgggtcag taacttctgg ctttgcgtcg atgatctcaa ataccctctg ggccgagtcc
     1801 acgcagcgag cccaccagtt ggatacccag gacataaact ccaggggtcc gtaaagcatc
     1861 gtcaggtagg aaatgaacga catcagtaca ccgatggtca taccttcggt tccgttcagc
     1921 accatgatac cgcctacacc ggttaccact acctgaccca agaacatgaa gagatagatc
     1981 agggggaacg cggtcgtaac cgtgttcatg gtgtgaatgt ccacaagggt ctgcttcccc
     2041 gacaccaccg aaaagcgctc cgtttcagtg tcttccttgg cgaaggcctt aatgaccctc
     2101 tggccattaa ctgcatcgct caccagagag ttgagctgcg agtggtagac ccagtttttg
     2161 tgatggaagc gtctgaagat actccacagc actcggaaaa acagaattgc caaaggaatt
     2221 atgaccagac agatgatagc aagttttgcg ttcaaaagga acatcaccag gaccacgcct
     2281 gtgaaggtga gaaggttcac cactacatac ggaagtccgt ctacaaagaa ccaatagatg
     2341 ttgttggcat cttggtttac cctgttcatc aggtttcccg tgcgctttga ggtgtagaat
     2401 cccacgtcca agcgctgcat ggcctcaaag atcttcatct tcaagtcctg cacgatccag
     2461 ggaacggtcc tagcgagaac gtaggagtac aggatgttca atcccgtgtt caccgctcgc
     2521 acgatgagaa tcatcaaaac cgctccgaga atgtagccgt agtagctgcc gccttctgta
     2581 aggacctcgt caaagaagaa cctgctgctt acaagaggcg tgatcatgga gaagacagat
     2641 ccagcaagca tcgtcacaaa aacccaaaac actttggttt tgtagtggcc aaaaaagcta
     2701 aagagcctct tggtgatgga gacgcgatcc aagcacttcg gacagatctt gcggactggt
     2761 tcggggtatc tggtaccgca tttggggcag aagagttctt cttctaccgg tagatcggtc
     2821 aggggatcct tcccctcgcg gatgttgctg atgaccttaa tgagtttttc tacagcacct
     2881 aaataaccga tggaaaagac caaaagaatg cttgtcttat cgtccttttc gctgcagagg
     2941 cgcactgttg aaaccaagcg ttccgttgaa aggttcccca catcttccaa gggaatgacg
     3001 tggagttctt tgggctcgta gaccgcaacg attctccgcg ccccggaagc ctttaccact
     3061 ttctcctcgc ctaggaggat gtagaggcta tcgctactta cagctaaata cgcgtccaga
     3121 tagccgctgt cgtctgaact gtcggcgtga actgcgtaga tcagctttga tgcgtcgatg
     3181 ccctgcgtct caaaaggcga gaccatgtac tccggaatca acgttttcat cgaaacttcc
     3241 tccctaaact cttccaaaac atccggacgt catcctagat gatcgtagta aaaatgctag
     3301 ttgtgctgtg ccagttttct tgaaattaca acgcatccgc ggactctacc gctcatattc
     3361 gctccccatc tacgtctacc tccaaagata gtgaaatttt tcttcaaaaa gcaaatttcg
     3421 tgaaaaaccc gttcgtgacc ctgccccatc atattgaagc atcaaattcg gtgaaagaaa
     3481 agggtatggt cggttcgtaa tcaactctcc cgaggcctta ctgcaggggc ttttagccca
     3541 ttgtcttcag cgtactccaa agccaaagct ccaggaagta ggactttcta ccgatttaac
     3601 ctgcggaagc tgcacttcca ctccagctcg gcaggtcaaa ggcaaaaact agccgttaaa
     3661 aaaaggtgcg ctcaagccac gcacgctt
//
LOCUS       DAOSDA010000140         3687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15915_length_3687_cov_3.863711, whole genome shotgun sequence.
ACCESSION   DAOSDA010000140 DAOSDA010000000
VERSION     DAOSDA010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15915_length_3687_cov_3.863711"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLW82_04225"
     CDS             complement(<1..483)
                     /locus_tag="PLW82_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46513.1"
                     /translation="MDKKLDVWFAVVAFLIIVLGSIGGIVPGLILLLLSFVYPPFSKD
                     FVGEVRGTTPQKRFLPVVTFFFVLWAVLATLAAAPGSGVAWGTVLGLVVVAFPVFYAG
                     VYAHNLDSRLVWRFFIWGAALAVAYIFYDHYVVGHRRAELLVGCNAAATLLLGSLGVF
                     L"
     gene            complement(520..1491)
                     /locus_tag="PLW82_04230"
     CDS             complement(520..1491)
                     /locus_tag="PLW82_04230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ46514.1"
                     /translation="MKILQVVPYSEIGGTEKAAALLSCGLAAKGHKVTVLCPEGPGRK
                     LFAGLELELLPQSFLQRRRLIRRLAPAYDLIHIHAARELVAAYQKPVVFTPHSYYNRL
                     DQLMVGVFAKKASVICFTRWEEQLLQDLGLRKTAVIPNGIGDAILPAERNEGFPLKIG
                     YMGRLAKDKGLDLLLRELAKVKEPYQLLIAGVGKEEEKLQAIAKELGIPAVFLGIQEP
                     AAFLSSIDIFVLPSRTETFGLALVEAMSAGLCCVAADVCGLKEILGDAGITVPHDELA
                     QALEKLLQDRGLRESLGKKARERFLSCYTEERMISDTEKFYVSLLNW"
     gene            complement(1597..2958)
                     /locus_tag="PLW82_04235"
     CDS             complement(1597..2958)
                     /locus_tag="PLW82_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ46515.1"
                     /translation="MQYDVIIVGGGPAGIFAALELSQKAKVLLLEKGRDLQKRSCPMK
                     VKNVQCVQCKPCSITSGWGGAGAFSDGKLTISTEVGGHLSEYVGETELRKLVDEVDKI
                     YLQYGAPDHLYTVEGKLLEQFKRDATKADLYLEYSPIRHLGTGRSQEVLLAMKDDLVS
                     RGVEIKTMLTVNSILTENGRVVGVSTDSGDFYAQNVIVAPGREGHNWFTKEAGRLGLK
                     TEMNPVDIGVRVELPKVIMEHLTDHLFESKYIYYSKTFEDKVRTFCMCPGGEVVAENN
                     SGLITVNGHTRSDRTTENTNFAVLVSKNFTEPFKDPTKYGQYISTLANLLGGGVLVQR
                     LGDLLDGRRSTRERMQKSTTRQTLTNATPGDLSLVFPYRHLVSILEMLQALDKVAPGV
                     YSRNTLLYGVEVKFYSSRVEVSNQLETAIKGLYAVGDGAGITRGLMQASCSGIIAARS
                     ILS"
     gene            complement(3082..>3687)
                     /locus_tag="PLW82_04240"
     CDS             complement(3082..>3687)
                     /locus_tag="PLW82_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ46516.1"
                     /translation="VPGRPNELAFNCPRISEKTDATNVDHLTNAQIEGRARIRRFMNF
                     FKKYFPGFENAYISSIASMVGIRESRRVRGEYYLTEDDHVNARKFPDGVAKNRYPIDV
                     HSSKKGGGINFYSHLKPGDYHEIPYRCLVPLEVENLLVAGRCISTSFLAQAAIRIEPN
                     CHALGEAAGVAAAMAIRENVTVRQLDGVKLRARLRENGANL"
BASE COUNT          874 a         1004 c          870 g          939 t
ORIGIN      
        1 cagaaagacg ccaagggagc ccagaaggag cgtcgctgcg gcgttgcagc ctacaaggag
       61 ctctgcccgc ctgtgcccga ccacgtagtg gtcgtagaag atgtaggcga ccgcaagagc
      121 cgcaccccag atgaaaaagc gccacaccaa ccgtgaatcc agattatgtg cgtaaactcc
      181 cgcataaaac accggaaatg caactacaac cagtcccagg acggtacccc atgccactcc
      241 actgccaggc gcggcagcca aagtcgccag tacagcccaa agaacgaaaa aaaacgtcac
      301 taccggaaga aagcgctttt ggggcgtggt gcctctcact tccccgacaa agtctttact
      361 aaacggtgga tatacaaacg acagcagaag gagaatcaaa ccaggaacga tgcccccgat
      421 acttcccaga acgattataa gaaacgctac taccgcgaac caaacgtcaa gctttttgtc
      481 caagtaatgc tagtcccctt tctatagtct tcattggctc taccagttca acaaagacac
      541 gtagaatttt tctgtatctg agatcattcg ttcttccgta taacagctga gaaacctttc
      601 tcttgctttt ttccccaaag actctctcaa acctctatcc tgaagcagtt tctctagtgc
      661 ttgagccagt tcgtcgtggg gaactgttat gcctgcatcg ccaagaatct ccttaagacc
      721 gcagacatct gctgccacac agcatagtcc tgcgctcata gcttcgacca gcgcaaggcc
      781 gaaggtctcg gttcgcgacg gcaggacgaa aatatcgatg gaactgagga aagcagctgg
      841 ttcttgaatg cccaaaaaga ccgcagggat gcctagttcc tttgcgatgg cctgcagttt
      901 ttcctcctct ttgcccacac cagcaataag cagctggtag ggttctttga ccttggcaag
      961 ttctctgagg agaaggtcaa gccctttgtc cttagcaagt cttcccatgt agccgatttt
     1021 aagggggaag ccttcattgc gctctgcagg taagatcgca tctccgatgc cgtttggtat
     1081 tacagcagtt ttcctcaaac cgagatcctg cagaagctgt tcttcccaac gggtgaaaca
     1141 gatgacagac gctttctttg caaatacccc aaccatcagc tgatcgagtc tgttgtagta
     1201 gctgtgtggc gtgaacacca caggtttttg atatgctgcc actagttctc ttgcagcatg
     1261 gatatggatt aagtcgtagg caggtgcaag ccggcggatc agcctcctcc tctgtaaaaa
     1321 gctctgtggg agaagctcta actccagacc ggcaaagagc ttccggcctg gcccttctgg
     1381 acacaagacc gttaccttgt gaccttttgc tgcaagtccg caagaaagca aagctgccgc
     1441 tttttcggtg ccaccgatct cgctgtaagg cacgacttga aggattttca ctttcctcca
     1501 ccaccactta aagcttataa gctaatcata gcttacgtga agcataatta aaagaaactg
     1561 caaaaaaacg cagtttcttt tgtgctactt aagctgtcag cttaagatac ttcttgctgc
     1621 gattatgcca gagcaggatg cctgcatcag tcctctggtg atgccagcgc cgtcgcctac
     1681 ggcatacagg cctttgatgg cagtttccag ctggtttgac acctctaccc tagacgagta
     1741 gaacttgacc tctacgccgt agagcaaagt gtttcgcgag tagactccag gtgccacttt
     1801 gtccaaggcc tgaagcatct ccagaatgct cacaaggtgc ctgtagggaa agactaacga
     1861 caggtctcct ggcgttgcat tcgtaagcgt ctgcctggtt gtgctctttt gcatcctctc
     1921 tcttgtagag cggcgcccgt ccaagagatc gccgagcctc tgcaccaaaa ctcctccgcc
     1981 gaggagattc gccagagtgc tgatgtactg tccgtactta gtgggatctt tgaaaggctc
     2041 ggtgaagttt ttgctcacca aaactgcgaa gttggtgttc tccgttgtcc gatcggatct
     2101 ggtgtgaccg ttgacggtga taagcccgga gttgttttct gcaacaacct ctccgcctgg
     2161 acacatgcaa aaagtcctga ccttatcctc aaaggtcttg gagtagtaga tgtacttcga
     2221 ttcgaacaag tggtctgtca ggtgctccat aatcactttt ggcagctcta ccctgactcc
     2281 gatatccacc ggattcatct cggtcttcag cccaagccta cctgcttcct tggtaaacca
     2341 gttgtgcccc tctctgccgg gagccacaat aacgttttgg gcataaaaat cgccgctgtc
     2401 ggtggaaacg ccaacgaccc tgccattttc tgtaagaatg ctgtttacag tcagcatggt
     2461 cttgatctcc acgccccggc tgactagatc gtctttcatg gccagaagca cttcctggct
     2521 gcgtccggtg ccaaggtgcc tgatcgggct gtattccaga tagagatcgg cttttgtggc
     2581 gtcgcgctta aactgctcca agagtttgcc ttcaacagtg tacagatgat caggagctcc
     2641 atactgcagg tagatcttgt ccacctcgtc cacgagcttc ctcagttcag tctcccctac
     2701 gtactcgctg aggtgtccgc cgacttccgt tgaaattgtg agcttcccat cggaaaatgc
     2761 tcctgcacca ccccatcctg acgtgatgga gcaaggttta cactgaacgc actggacatt
     2821 ctttaccttc atgggacagc ttctcttctg tagatcgcga cctttttcca gtagaagaac
     2881 cttggctttt tgcgacagtt ccaaagcggc aaaaatgcct gctgggccgc ctccaactat
     2941 aatgacatcg tattgcataa taactcctcc tggcacatga aaaagctaga taaactctat
     3001 tcttcggcaa aaggagcaag cctgcaggtt tagatgcaga cttgcttatt tgcatatcta
     3061 atatttctaa caggcttcag cctaaagatt ggctccgttt tctctgagcc ttgctcttag
     3121 tttaactcca tcgagttggc gcactgtgac gttttctctg atcgccatcg ccgccgctac
     3181 tcccgcagct tcgccaagag cgtggcagtt cggttcgatt ctgatcgcag cctgggccaa
     3241 aaacgatgtg gagatgcaac ggccggcaac gagcagattc tcaacttcca gcggaactag
     3301 acacctgtag ggaatctcgt ggtagtcgcc tggtttgagg tggctgtaga agttgattcc
     3361 gccacctttc ttagagctgt gaacgtcgat cgggtagcgg ttcttagcta caccatcagg
     3421 gaacttcctg gcatttacat gatcgtcttc tgtaaggtag tactcaccgc ggacccttct
     3481 ggattccctg atgcccacca tactagctat ggaagagatg taggcgtttt caaaaccagg
     3541 gaagtacttc ttaaaaaagt tcatgaagcg cctgatcctg gctctgcctt cgatctgggc
     3601 attggtcagg tggtcgacat ttgttgcatc ggtcttttca ctgattctcg ggcagttaaa
     3661 cgccagctca ttgggtcttc ctggaac
//
LOCUS       DAOSDA010000141         3680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15956_length_3680_cov_3.184276, whole genome shotgun sequence.
ACCESSION   DAOSDA010000141 DAOSDA010000000
VERSION     DAOSDA010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15956_length_3680_cov_3.184276"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="PLW82_04245"
     CDS             <1..1486
                     /locus_tag="PLW82_04245"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOJ46517.1"
                     /translation="WEESGRFQSVGSELLRFNDRTGQDFVLGMTHEEAAVHLARGEAK
                     SYTKYPFMIYQIQTKFRDEPRSRGGLIRVREFTMKDAYSFHTSQEDLESYYDEVLDAY
                     VRIYKRAGLGNVKVIKSDTGMMGGSTAHEFMYICDGGEDSIVYCESCGYAANMEVAVS
                     TPPAPIVGEEKEIQEVHTPGIKDIESLAEFLGVLPMQTIKAAVFARKDNNKPVVVFVR
                     GDLEVNETKVTKVVKAEIYPLTDYSESDLDFGFIGPVGLKAEGAEVIFDKSLDGLQNM
                     VCGANKTDYRLKNVSVPRDLKDVKFVDVVSVGEGHSCSFCGNPLKITRGIEVGNIFQL
                     GDKYTKSMNMTYLDQNGRLQTPIMGCYGIGVGRLLACIIEEHHDQYGPIWPITVAPWQ
                     VHICILNNNVEEVRNTGMDLYHKLGSKFEVIVDDRNVSAGFQLADADLIGVPVRVIVS
                     KRNLENGEVEVVTRDKSTKVNVKLEEVEAFISDLIAKMEAEINS"
     gene            1793..2320
                     /gene="dcd"
                     /locus_tag="PLW82_04250"
     CDS             1793..2320
                     /gene="dcd"
                     /locus_tag="PLW82_04250"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOJ46518.1"
                     /translation="MILSGLEIKKHIGKEIIIDPFDEKNLNPNSYNLRLHNELLVYDE
                     PVLDMKKPNKTKKLVIPEEGLVLEPGRLYLGRTLEYTKTDKFVPMLEGRSSVGRLGLY
                     IHVTAGFGDVGFSGYWTLEIQCVQPIRIYAGVEICQIYYHLIMGEYEKYSSGKYQNNS
                     GIQPSLLYMDFANKK"
     gene            2458..>2815
                     /locus_tag="PLW82_04255"
     CDS             2458..>2815
                     /locus_tag="PLW82_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOJ46519.1"
                     /translation="MFKIGDYVVYNLTGVYRIVDIRTEKDIENRDTQYYILRPAFGGN
                     LTVKIPTDCRKVYMRQVGSREDILELMAGIPDTETAWIDDARERNHTFKSELNSGECR
                     SWVKLVKTLYLQREEKS"
     assembly_gap    2816..2825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3126..>3680
                     /locus_tag="PLW82_04260"
     CDS             3126..>3680
                     /locus_tag="PLW82_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ46520.1"
                     /translation="MKTLALDFAKYVSLNVLGMIGLSCYILADTFFIAKALGPLGIAA
                     LNFSISVYSIIAGVGLLVGIGGGARYAILQSQKKHEEANRVFTSAVVLGAVLGAGLFV
                     VGVFGTRQLAVLLGADADVFPMTETYLRTILVFAPAFIANNILLAFVRNDENPRLPMI
                     AMLTGSASNVVLDYVFMFPLQMGMF"
BASE COUNT         1003 a          836 c          976 g          855 t
ORIGIN      
        1 ttgggaggag tcggggaggt tccagagcgt cggcagcgag ctcttaaggt ttaatgatag
       61 gactggccag gatttcgtgc tggggatgac ccacgaagaa gccgccgtgc atcttgcccg
      121 gggcgaggcc aaatcatata cgaagtatcc attcatgatc taccagattc aaactaagtt
      181 ccgcgatgag cccagatcca gaggaggcct cattcgggtc agggagttta cgatgaagga
      241 tgcctattcc ttccacacct cccaggagga tctagaaagc tactacgatg aagtcctcga
      301 tgcctatgtg aggatctaca aacgggcagg tctcggaaac gttaaagtca tcaaatccga
      361 cactggtatg atgggcggaa gcaccgctca cgagtttatg tacatctgcg acggtgggga
      421 agactccatc gtctactgcg aaagctgcgg ttatgcagct aacatggaag tcgcagtctc
      481 aacgcctccg gcgccgatcg tcggtgaaga aaaagagatc caggaggttc acactcccgg
      541 catcaaggac atcgaaagcc ttgcagagtt tttgggagtt ttgcccatgc agaccatcaa
      601 agcggcagtg tttgctagaa aagataacaa caaacctgtc gtggtgtttg tcaggggcga
      661 cctcgaggtg aacgagacca aagtcactaa ggtggttaag gcggagatct atccccttac
      721 agactactca gaaagcgacc tggacttcgg ctttatcggt cctgtgggct tgaaggccga
      781 gggcgctgag gtgatcttcg acaagtctct tgacggtctg cagaacatgg tgtgcggtgc
      841 caacaaaacc gactaccgcc tgaagaacgt cagcgtacct agagatctta aagacgttaa
      901 gttcgtggac gttgtcagcg taggggaagg gcacagctgc agcttctgcg gtaatccgct
      961 gaagatcaca agaggtattg aagtcggaaa catcttccag cttggcgaca agtacaccaa
     1021 gtccatgaac atgacctatc tggaccaaaa cggcaggctc cagacgccga ttatgggctg
     1081 ctacggcatc ggcgtaggca gactccttgc ctgcatcata gaagagcacc acgaccagta
     1141 cggcccgatc tggcctatta ccgtggcacc gtggcaggtc cacatctgca tcttgaacaa
     1201 caacgtcgaa gaggtacgaa atacaggaat ggacctctat cacaaactcg gcagcaagtt
     1261 tgaggtaatt gtggacgacc gcaacgtgtc cgctggtttc cagctggcag atgccgacct
     1321 tatcggtgta cctgttagag taattgtcag caagaggaat ctggaaaatg gagaagtaga
     1381 ggtagtaacc agagataagt ccacaaaggt caacgtgaaa ctagaagaag tggaagcgtt
     1441 cattagcgat cttattgcaa aaatggaagc agaaatcaat agctgaaaag tgggaaggtg
     1501 gtcacccccc attccgctgt ttttttggtg agtatccatt ttactcgacg ggctcaagcc
     1561 tcgcgttgaa gcccgtcgat tttttatcga cgaaaccttg aggaactaag agttcctaat
     1621 aagagagata cttttccatt gacggtgaca gggaatggac gcccgaaaga aagcaatagt
     1681 ggcaaggcag aggtagactt cgatggagcc tgtcagtcaa tcgacgttgg ttaaacctaa
     1741 gtaaactggg tatgtgattt gaagaataga ggcagaattg ggggagatag acatgatact
     1801 ttcaggacta gagatcaaaa aacacattgg caaagaaata atcattgatc ctttcgacga
     1861 aaagaacctt aatcccaaca gctacaacct aagacttcac aacgaacttc tcgtctacga
     1921 tgaacctgtt ttggacatga aaaagcccaa caagaccaaa aagcttgtca ttccagagga
     1981 aggtctggtt ctagagcctg gaaggctcta tctagggcgg actttagagt acacgaaaac
     2041 cgacaagttt gtgcccatgc tggaagggcg ttcttcggta gggcgactgg ggctctacat
     2101 ccacgttaca gcagggtttg gggacgtcgg gtttagtgga tactggactt tggagatcca
     2161 gtgcgtgcag cccatcagga tctacgccgg agttgagatc tgccagatct attaccacct
     2221 gattatggga gagtacgaaa agtattccag cggtaagtac caaaacaaca gcggaatcca
     2281 gccaagcctc ctctacatgg actttgcgaa caagaagtaa acttcggtta tcatctaatg
     2341 tgtcttctta cacgggacct ccttcggtta atttgaggcg cttagggcat actgaaactg
     2401 caccctgtct tgcctgatgc aggaagatat cgccaagcaa gctctaggag gatgcctgtg
     2461 tttaagatag gcgactacgt agtctacaac cttaccggtg tatacaggat cgtggatata
     2521 cgtacggaaa aagatataga aaaccgtgat acccagtact acatcctcag acccgctttc
     2581 ggcggcaacc ttacggtaaa gattcccaca gattgccgca aggtgtatat gaggcaggtt
     2641 ggaagccgtg aggatatttt ggaactgatg gccgggattc ccgacacgga aaccgcttgg
     2701 atcgacgatg ccagagaaag aaatcacacg ttcaaaagcg agttgaactc tggtgagtgc
     2761 agaagctggg tcaagctcgt taaaactctg tatcttcagc gagaggagaa gagtcnnnnn
     2821 nnnnnatgaa aaccgacgaa gagatcatga aagcagcaga gaagaacctc agcgaagagg
     2881 ttgctatcgc cctcagcatc tctcctgacg aggtccccgc catgatcctt ggttacatgg
     2941 agtagtgcta aaggagtgcg accttgccgc aagaatgagg gctgttgact gattcggtag
     3001 atttccttag aatgagataa actgtatcta cggctcttta cgtagggcag agcaacggaa
     3061 gtgcttgtgg cattccctat accttaaaaa ggaatgctcg gggcactttt tctttggagg
     3121 atagaatgaa aactttagct ctagattttg caaaatacgt aagtttgaac gtactaggca
     3181 tgattggcct ttcctgctac atcttggcag acactttctt catagctaaa gctctaggcc
     3241 ctttaggcat tgcagcgctt aacttttcga tttccgtcta cagcataatc gccggcgttg
     3301 gcctgcttgt gggaatcggc ggcggagcca ggtacgcgat tttgcagtcc cagaaaaaac
     3361 atgaagaggc aaaccgagtt ttcacctctg cagttgtctt gggagctgtt ttaggagcag
     3421 ggctgtttgt ggtcggggtg tttggcacaa ggcagcttgc ggtgctcctg ggagccgatg
     3481 cagacgtttt tcccatgacc gaaacctact tgcgtaccat tctcgtcttc gcaccggctt
     3541 ttattgcaaa caacattctg ctggcatttg tcagaaacga cgaaaacccg aggctcccta
     3601 tgatagccat gttgacaggc agtgcatcta acgttgtttt ggactacgtc ttcatgtttc
     3661 ccctacaaat gggtatgttc
//
LOCUS       DAOSDA010000142         3679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15959_length_3679_cov_3.406733, whole genome shotgun sequence.
ACCESSION   DAOSDA010000142 DAOSDA010000000
VERSION     DAOSDA010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15959_length_3679_cov_3.406733"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLW82_04265"
     CDS             complement(<1..257)
                     /locus_tag="PLW82_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009802485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOJ46521.1"
                     /translation="MELLICTHNEHKVDEIAGFLQGYKLRSFKDLAGFTPPEETSLLF
                     QGNALIKAAAGFRASGLPSLADDSGLEVLALGGKPGVFSAR"
     gene            complement(269..997)
                     /gene="rph"
                     /locus_tag="PLW82_04270"
     CDS             complement(269..997)
                     /gene="rph"
                     /locus_tag="PLW82_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOJ46522.1"
                     /translation="MRIDGRASDQMRPVIITPNFLKNPHGSALVEFGDTKVICTAMVE
                     DKVPPFLRGTNQGWLTAEYEMLLGATLQRTVRDASKGRISGRNHEIQRLIGRSMRSIT
                     DLAALGERTVWLDCDVIQADGGTRTASISGAFVALVLALNRFRESLQLEKLPIRSYIG
                     AVSAGIVNDVPLLDLCYEEDSTAMVDMNFVLTSDNQIVEIQGTGEARPFNRDEMQTLL
                     ELAEKGVKVIFDAQKAVIGDLLDI"
     gene            complement(1126..2517)
                     /gene="murD"
                     /locus_tag="PLW82_04275"
     CDS             complement(1126..2517)
                     /gene="murD"
                     /locus_tag="PLW82_04275"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOJ46523.1"
                     /translation="MDLNFKGKNVAVIGLGTENIPLIKYLKDAGANVCGRDMKNSDKL
                     GERYQTLLNLGIELRLGLGYLEGLTQYDIVFLTPGIPRSLPELVEARRKGVRFSSQME
                     LFFNICPATIIGITGSSGKTTTTTLVGNMLSGSGFDTRIGGNIGRVLLSEVMDMTPRT
                     KVVLELSSFQLQDMKISPEIALITNITPNHLDVHKDMHEYVEAKKQIYLHQTSENAVV
                     FNAEDPLSTGFDKEAPSRVYYFSKRPLPEGLSGAFIRDGWIVIRDLNAVTKELLVCSI
                     NDIRLLGLHNQENVLAASLVAYLVGAKIEAIAQAIREFKGVDHRLEYVATIGGVRYYN
                     DSIATSPARAKAGLLSFSEPVVLIAGGYDKKIPFDELREAVLQKRPKAVVTVGDTSQK
                     IAEALFGLAPIYHAETFEKAVDTASSLADPGDCVLLSPACASFDMFASYEERGKEFRR
                     LVLKKAEELNPQS"
     gene            complement(2588..>3679)
                     /locus_tag="PLW82_04280"
     CDS             complement(2588..>3679)
                     /locus_tag="PLW82_04280"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ46524.1"
                     /translation="PMLFYQSQTGLPEYREYLDSDLRVVRAFMGVKLLGEAKRVDVND
                     DLIDYYEYFQDGPSVVAVMKIKPALQGFFSQSVQGKRLAQILKIRSEENDIGTEILFE
                     VMGNPYARARKEGSRVIVEFSDTQANSVVRDLRYTSEYSGLTLTKLTDDSINVEILLK
                     DAKGYALTQKDEYTYSVQLVKVGLQAKLVVIDPGHGGYDPGATGVFLEEKDLNLDVAF
                     RLRDLLENLGYMTILTRETDLYVSLSERTNLSRNSGADLFVSVHTNGSTNSSAKGIET
                     YYAPQSSEGKKLAESVQKALIVHTQAADRSAKDAGFWVLVNNIPRAILVEIGFVTNPS
                     EEQKLNDENYRQKIAEGICSGIISFMMQK"
BASE COUNT          853 a          974 c          835 g         1017 t
ORIGIN      
        1 taccgggcag agaacacgcc gggcttgcct cctaaggcga ggacttcaag ccccgagtcg
       61 tctgcaagag acggtaggcc gctcgctcta aatccagcag cggctttgat taaggcattg
      121 ccttggaaaa gcagcgatgt ttcttccgga ggcgtaaaac ctgctaaatc cttaaaactt
      181 ctgagcttgt acccttgcaa aaagcctgcg atttcatcaa ccttgtgttc gttgtgtgtg
      241 cagattaaaa gttccatgtc atccaccctt atatgtccaa tagatctcct attactgctt
      301 tctgcgcgtc aaagataacc tttacgcctt tttcagcaag ttccaacagt gtctgcatct
      361 catctctgtt gaacggcctg gcttcgcctg tcccttggat ctcaacgatc tgattgtcgg
      421 aagtcagcac gaagttcata tccaccatcg ctgtagagtc ttcttcgtag catagatcca
      481 ggagcggaac gtcgttaacg atgcctgccg acactgcccc gatgtagctc ctgatgggaa
      541 gcttttccag ctgaagagac tctctaaagc gattgagggc taaaaccaag gctacaaaag
      601 cgccgctgat agaagctgtg cgcgttcctc catcggcttg gatcacatcg cagtccaacc
      661 acactgttct ctcgcctagt gccgccagat cggtaataga acgcatggac ctgccgatca
      721 agcgttgaat ttcgtgattt ctgccgctga tcctgccttt agacgcgtct ctaacagtcc
      781 tctgcaacgt ggcgccgagc agcatctcgt actctgcagt gagccagcct tgattggtcc
      841 cgcggagaaa cggcgggact ttatcctcga ccatagccgt gcagataacc ttagtatcgc
      901 caaactcgac taaagcagaa ccgtgagggt tcttcaggaa atttggtgtt ataatcactg
      961 gacgcatttg gtcagaagct cttccatcga ttctcatagt gtttctctcc ctttctcaag
     1021 atacctgctc tacttgacaa cacaaactga acacaccttt ttcttcgtct tttccttcta
     1081 cgatgggtag aaactgctct tgtggcaagc ttgcttttcc agatctcagc tttggggatt
     1141 tagttcttcg gcctttttca gaacaagacg cctaaactcc ttacctcttt cttcgtagct
     1201 ggcaaacata tcaaagctgg cacaagcagg cgaaagaagt acgcaatctc ctgggtctgc
     1261 caacgaactc gccgtgtcaa ctgctttttc aaaagtctcc gcatgatata taggcgcaag
     1321 cccaaacaac gcttctgcaa tcttctggct agtgtctcct actgttacaa cagctttcgg
     1381 cctcttttga agcaccgctt ccctgagctc gtcgaagggt atttttttgt catagcctcc
     1441 tgcgatgaga acgacaggtt cggaaaagga aagaagcccc gctttagccc ttgcaggcga
     1501 ggtggctata gagtcgttgt agtagcgtac gcctccaata gtggcgacat actccaaacg
     1561 atgatctacc cctttaaact cccgaatagc ttgggcgata gcttcgatct tcgcacctac
     1621 taagtaagca actaggcttg cagccaaaac gttctcctga ttgtggagac caagcagcct
     1681 aatatcgttt atactgcaga cgaggagttc tttagttact gcgtttaaat cgcggatcac
     1741 aatccatcca tctctaatga aagcccccga caaaccttct ggcaaaggcc ttttgctgaa
     1801 gtagtagacc ctgcttggag cttctttgtc gaaccccgtg ctcagaggat cttcagcgtt
     1861 aaaaaccacc gcgttctcgg acgtttggtg caggtagatc tgtttttttg cctctacgta
     1921 ctcatgcata tccttgtgga cgtccaggtg attgggagtg atgttagtga tcagagctat
     1981 ctccggcgat atcttcatgt cctgaagctg gaagctggag agctcaagca ctaccttggt
     2041 tcgcggcgtc atgtccatta cttcggacag gagaaccctg ccgatgttgc cgccaatccg
     2101 ggtatcaaaa ccggaacctg aaagcatgtt ccctactaag gtagtggttg tggttttccc
     2161 agaactaccg gttatgccga tgatcgttgc aggacagatg ttgaaaaaca gctccatctg
     2221 gctggaaaag cgtacacctt tcctcctggc ttccactagc tcaggcagag atctcgggat
     2281 tcccggtgtg agaaacacta tgtcgtattg ggttaggccc tcaagataac ccaatccaag
     2341 cctcagttcg atgcccaagt tgaggagagt ctgatagcgt tctcccagtt tgtcggagtt
     2401 tttcatgtcc cgaccgcaaa cgttagcccc ggcgtcttta aggtacttga taagggggat
     2461 gttttcagtt ccgagcccga ttactgccac gtttttgcct ttaaaattca gatccattgt
     2521 ctcctgccca ccttcttaaa cgtcttgtca accctaacta tacttatatc gctgttcttt
     2581 cagctgctta cttctgcatc atgaagctga tgattccact gcagatacct tcggcgatct
     2641 tctgcctgta gttctcatcg tttagttttt gctcttcaga gggatttgta acaaagccaa
     2701 tctcaaccag gattgccctg gggatgttgt ttacaagaac ccagaagccc gcatccttcg
     2761 ctgaacgatc cgcagcttgg gtgtgtacaa tcagcgcttt ctgcactgac tctgccagtt
     2821 tctttccttc actgctttgc ggcgcgtagt acgtttcgat cccctttgcg gaactattag
     2881 tactgccgtt ggtgtggacg ctgacaaaaa ggtctgcacc gctattgcgg gagaggttgg
     2941 tcctttcaga aagcgataca tacaagtctg tttcccttgt gagaatggtc atgtacccaa
     3001 gattctccaa gagatccctt aagcgaaaag ctacatccag gttgaggtct ttctcctcca
     3061 aaaaaactcc tgtagcgcca ggatcgtaac ctccgtgccc ggggtctatg acaacaagct
     3121 ttgcctgaag gcccacctta accagctgga cactataagt atattcgtcc ttttgggtta
     3181 gtgcataccc tttggcatcc ttaagtaata tttcaacgtt gatggagtcg tctgtgagtt
     3241 tggttaaggt gagtccgctg tactcagagg tgtaacggag atctctcact acactgttgg
     3301 cctgagtgtc gctgaactca acgatgactc ggctaccctc ttttcttgcc ctagcatagg
     3361 gattgcccat tacctcaaag agtatttcgg ttccgatatc gttttcttca gacctaatct
     3421 tcaagatctg cgcaagcctt ttcccctgaa cgctttggga gaaaaaccct tggagcgcag
     3481 gcttgatctt catcaccgca acaaccgacg ggccatcctg gaagtactca taatagtcga
     3541 tgaggtcgtc attgacatcc actctcttag cctctcccaa aagcttgacg cccataaatg
     3601 cgcgaacgac tctgagatcg ctgtcgagat actctctgta ctcgggaagt ccagtttggc
     3661 tttggtaaaa gagcatggg
//
LOCUS       DAOSDA010000143         3675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_15978_length_3675_cov_3.598343, whole genome shotgun sequence.
ACCESSION   DAOSDA010000143 DAOSDA010000000
VERSION     DAOSDA010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15978_length_3675_cov_3.598343"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(497..733)
                     /locus_tag="PLW82_04285"
     CDS             complement(497..733)
                     /locus_tag="PLW82_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46525.1"
                     /translation="MVILSGCIPGDGKHTEERPAGFFWGIWHGWTAPISLIGGLFNKN
                     LRVYEVFNTGWWYDFGFYVAIIAGFGGLSLTRKK"
     gene            complement(847..1674)
                     /locus_tag="PLW82_04290"
     CDS             complement(847..1674)
                     /locus_tag="PLW82_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOJ46526.1"
                     /translation="MKTQRVIKRDATYQKFEVLKSNRNKRHRYGEFFVEGVRNINHLI
                     SEGWTVNSFLYSGEAKLSDWARDILKAVPTKVNYELTDELMAELSGKTDASELMAIVQ
                     MRPNDPAQIRPGKNPLLVLFDRPSNRGNLGTIIRSIDAFGLDGLVVTGHSVDLYDPET
                     IVASMGSFFKVPSVRIEDNQSIREWIQGLKAKHPGFQVVGTSAHAECSLFDADLTAPT
                     LLLIGNETDGLSRKLAEISDRMVKIPMSSSSSASSLNVACAASILFYEAIRQRKGTL"
     gene            1938..>3675
                     /locus_tag="PLW82_04295"
     CDS             1938..>3675
                     /locus_tag="PLW82_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46527.1"
                     /translation="MKLLWRLGKEAGKYKGLYVLAILSTLLLTLINLVAPKVLSNMTA
                     VIEHRVSENPVREIAVLTGILIGLYLAKILFRFLASYLSHKAAWELVQEVRVRVYNHI
                     QNLSMGFFADKQTGDLMSRVVNDTATFELLYAHIIPEMITNVVTVVGVMVVLLTVNVK
                     LALLTCIPIPFILFSGWIFSTKIRPNFRVSQKALADFNSKLQDNFSGIQEIQAFGQEN
                     YEKSAVESKASSYTKAILHALKLSAVFHPTVEFLSSVGTIIVVGVGGVFALRGQLSVS
                     DIVAFLLYLSLFYAPIAGLARLLEEAQQAYAGAERVMAILDTPFEIQNAPDAIELKEV
                     KGSIAFESVYFEYEKGVPVLKNINFSCKPGEMIALVGPTGVGKTTLTQLIPRFYDPTS
                     GRVLIDGIDVRNVTLESLRANISPVLQDTFLFNGTIAENIGYANPKATFEEIVEAAKA
                     ARIHDDIMEMPDKYNTRVGERGIRLSGGQKQRIAIARAILRKSPIIILDEATASVDVE
                     TEKEIQKAISDLAGTRTIIAIAHRLSTIQNADMILVLEEGTIVQRGTHQELMAQEGLY
                     RRLNMAQAQALPG"
BASE COUNT          924 a          991 c          843 g          917 t
ORIGIN      
        1 acatcgactt gaagggaaaa agcgccgttt attttggcac ccatgtgtct gaggtaaaag
       61 ctcttgaact cacgaagcct ttctgcactt agtacctcaa ctctcattta cccaatgctc
      121 cttcgcagct atatggttag acctaaaaga ggtcttttat gcctgccggc tgatacgtca
      181 aaactccgag cgcctgcggc aaaaaagacc gttggatagt ctattctaat ttcgtgtact
      241 ttgtctataa tgttcacgcc ttgattaggc atcctctgta gcaaaaccac ccatttcgta
      301 aacagattat cagaaagcaa gctgctacgc tataggtctt ttgtcctgct tgtcagtgca
      361 attctggcaa gtgcaattag atcctcacca gtgtctcctc caaagaggtt accccctagt
      421 gcctgaaaag atcttatcta aagaaaaaag ccgcatcttt ctttgggaaa gacacgacct
      481 atccccattt ctactgttat ttcttcctgg ttagggaaag cccgccgaat cctgcgatga
      541 tggctacata gaaaccgaaa tcataccacc aaccggtgtt gaacacttcg taaactctga
      601 ggttcttgtt gaacaacccg ccgattagag atatgggtgc ggtccaaccg tgccagatcc
      661 cccaaaagaa tcctgcaggt cgctcctctg tgtgcttgcc gtctccggga atgcagccgg
      721 aaagaattac catcgataga agtaacacca agatccaaat cagctttttg ctcactgcaa
      781 aacctccttc gcttccgcgc tttctacttc tatatactca agttctccag aaaagtttca
      841 cgcccttcaa agggttcctt ttctctgcct aattgcttcg tagaatagaa tcgacgcagc
      901 gcaggccaca tttaacgacg acgccgagct ggagctagac atgggaattt tcaccattcg
      961 gtcgctgatt tccgccagct tcctgctgag tccgtcggtt tcatttccga tcaggagcaa
     1021 agtcggcgcg gtgagatccg catcaaacag cgagcactct gcatgagcag atgttcccac
     1081 gacctgaaac cccggatgct tggcctttaa accctgaatc cactctctga tggactggtt
     1141 atcttctatg cgcaccgaag gaactttgaa gaacgaaccc atggaagcta ctatggtttc
     1201 gggatcgtag aggtccacgc tgtgaccggt gactaccaga ccgtcgagac caaaagcatc
     1261 aatggagcgg atgatcgttc ccaggttccc ccgattggac ggcctgtcaa acaagacgag
     1321 gagcggattt ttgccgggcc gaatctgagc cgggtcgttc ggcctcattt gcacaatggc
     1381 cattagctct gaggcatcgg tcttgccgct gagctctgcc atcagttcgt ctgtgagctc
     1441 gtagttcact ttagtaggca cggctttgag gatgtccctc gcccaatcgg aaagcttcgc
     1501 ttcacctgag tagaggaagg aatttaccgt ccaaccttcg ctgataaggt ggttaatgtt
     1561 ccgaacccct tcaacgaaga actctccgta acggtggcgt ttatttctgt tgcttttcag
     1621 aacttcaaac ttctgatagg ttgcatctct tttaataact cgctgggttt tcatagcatt
     1681 gcactcccaa aatctcaggt tttaacctct tctctgtgtt ctctacttag cttccaattc
     1741 cctttaacgc ggctagttag cttactgcca aacttctccg caagaagctc cggagaagca
     1801 caattacttg agttttcgag agccttgcag gaattcactc cgtggtgcac agaatagaga
     1861 attactgata gaactgtttg ttagtctatg gcagcagaac ctaactggaa aactaccaaa
     1921 acatggagga cgtgcttatg aagcttttgt ggcgcctcgg gaaggaagca ggcaagtata
     1981 aggggctgta cgtcttagcc atcttgtcaa cgcttctcct tacgctcatc aacctcgttg
     2041 caccaaaggt gctttctaat atgacggccg ttatagaaca tcgggtgtca gaaaatcccg
     2101 tacgggagat cgcagtgctc actgggattc tcatcgggct gtaccttgcc aagattttgt
     2161 ttcgcttcct ggcaagctac ctatcccaca aagccgcttg ggagctggtg caggaagtta
     2221 gggtccgcgt ctacaaccac attcagaacc tctcaatggg cttttttgcc gacaagcaga
     2281 ccggagacct gatgagccgg gtggttaacg acaccgcaac cttcgagctc ctttatgccc
     2341 acattatccc cgagatgatc actaacgtag ttaccgtcgt tggtgttatg gtggtactgc
     2401 taacggtaaa cgtcaaactc gcacttttaa catgtattcc tattcccttc attctctttt
     2461 cggggtggat cttctccacc aagatccggc cgaacttcag ggtgtcccaa aaagctctgg
     2521 cagacttcaa ctccaaactc caggacaact tctcgggcat ccaggagatc caggctttcg
     2581 gacaggaaaa ctacgaaaag agcgctgtgg agtccaaagc cagctcatac actaaagcga
     2641 tcctccacgc cttaaagctc agtgcggtct tccaccctac tgtagagttt ctctcctcgg
     2701 taggaaccat aatcgtcgta ggggttggag gagtctttgc tctgagagga cagctttcgg
     2761 tctccgacat agtcgccttt ctcctctacc tctcgctctt ttatgcacct atcgccggtc
     2821 ttgcaaggct tctagaggaa gctcagcagg cctacgcagg agctgaaagg gtcatggcca
     2881 tcctggacac gcctttcgag atccaaaacg cacctgatgc gatagaactg aaagaggtca
     2941 aaggatcaat tgccttcgag tcggtctact tcgagtacga aaaaggcgtt ccggttctaa
     3001 agaacatcaa cttctcctgc aaacctggag agatgattgc cctggtaggc cctacaggcg
     3061 taggcaaaac cactctcact cagctgatac ccagattcta cgatcctaca tcaggaaggg
     3121 tgttgataga tggcatcgat gtcagaaacg tcactctaga aagcctccgc gctaatatct
     3181 cgccggtgct ccaggacacg ttcctcttta acggcaccat cgccgagaac atcggctacg
     3241 ccaaccctaa ggccacgttc gaagagattg tggaagcggc taaagctgca aggattcacg
     3301 acgacattat ggaaatgccc gacaagtaca acaccagggt cggcgagcga ggtatccgtc
     3361 tttccggagg tcagaagcag aggatcgcca tcgccagggc tattctgaga aagtctccta
     3421 tcatcattct cgatgaagcc acggcttctg tggacgtgga aacagaaaaa gagattcaaa
     3481 aggccatcag cgaccttgca ggtaccagaa ccattatcgc catcgcccac aggctttcca
     3541 ccatccaaaa tgccgacatg attttggtcc ttgaagaagg caccatcgtc caaaggggca
     3601 cccaccaaga gctaatggct caggaaggtc tctacagacg ccttaacatg gcgcaagccc
     3661 aagcacttcc cgggc
//
LOCUS       DAOSDA010000144         3671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16008_length_3671_cov_3.121405, whole genome shotgun sequence.
ACCESSION   DAOSDA010000144 DAOSDA010000000
VERSION     DAOSDA010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16008_length_3671_cov_3.121405"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLW82_04300"
     CDS             <1..639
                     /locus_tag="PLW82_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HOJ46528.1"
                     /translation="SFTESKKGLVDLWYKRYLKAKAEGTDLQDMYKLSPAWRTFWQRL
                     EGRFTMDEDTKMYVTDSHLWSYKIAKKHDCRTVCLFDSHADLGYGGLSSLDFEVNCSN
                     WLGRLLKDEHSENAHIVYSPHTLEKPEHFQEINSMYTITYYTNLDDLDGPFNVSAIHV
                     CRSGAWTPPWFDDKFVMFVEALGKEHEVIDCPKRKWDTKSLSLSRQIDYLFA"
     gene            916..2085
                     /locus_tag="PLW82_04305"
     CDS             916..2085
                     /locus_tag="PLW82_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOJ46529.1"
                     /translation="MHIQEIIRAFMLIFIAEMGDKTQILAMAFVTRYPVRKVLIGIGL
                     GAFLNHGLAVVLGNYLSRVIPISTVQMIAGIAFIGFALWTLKPEQEEADKEAKVEFGP
                     VATVAMAFFLGELGDKTQLTAITLAADSTSPMTILAGTVLGMVATGALGIYIGKKLGD
                     KIPELGIKVLASCVFAFFGVQKLLQTVPAQYLNVYTVVPSIGLLGLVMLGMIRSLIVR
                     KRSGIQSEFLTTAKLLRNYYLHLQEDLDHICLGAEYCGTCAGRMCAIGKSKEIVKAGL
                     VNEEWHDLPQNGGVYHNNKPFKQEEVLDCLVDTLCLIGTVPDEKDLANAHLIRNQMEE
                     ILLGHHIESFESIKSYIEDLREVDAVLSEKIAKMVRVRNTAKQPAETAMESPRHV"
     gene            2630..3295
                     /locus_tag="PLW82_04310"
     CDS             2630..3295
                     /locus_tag="PLW82_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOJ46530.1"
                     /translation="MAKYVCSVCGFEYDEAIGIPEAGIAPGTKWEDLPEDWVCPVCGA
                     EKSDFEMEGSVDSTPQIVEAAVAADPDMKELSPLEVSALCSNLARGCEKQYKFEEAEL
                     FSELAAFFKSKAVPAKDPDFSQLFELVEKDLEQGIPNANSAAEAVRDRGALRALVWNE
                     KVTRILKSLLTRYQKEGDALFANTGIYVCTICGFIFIGDKLPDVCPICKVPNWKFEKV
                     EGR"
     gene            3301..>3671
                     /locus_tag="PLW82_04315"
     CDS             3301..>3671
                     /locus_tag="PLW82_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ46531.1"
                     /translation="MTTKYAVRNIRLCTKDCLCLFVCPTGATDTENSIIDVEKCIGCG
                     DCADACPSGAISMVPFEYPTQQPKTETVVGAMNALLRSKSEQENIAASLPGILAKAIE
                     KSNRIMAEDILREAGFMLPQS"
BASE COUNT         1026 a          807 c          934 g          904 t
ORIGIN      
        1 tcctttactg aaagcaaaaa aggtttggtt gatctttggt acaaaaggta cctgaaggcc
       61 aaagccgaag gaacggatct gcaggatatg tacaaactat ctcctgcatg gagaacgttt
      121 tggcagcggc tagagggaag attcaccatg gacgaagaca caaaaatgta tgttacagac
      181 tcccaccttt ggtcttacaa gattgccaag aagcacgact gccgaactgt ctgcttattt
      241 gactcccatg ctgatcttgg gtatggagga ttatcttcac tggatttcga ggtcaattgc
      301 tctaactggc ttggcaggct acttaaagat gaacatagcg aaaacgctca cattgtttac
      361 agtccacata cgctggaaaa gcctgagcac ttccaggaga taaacagcat gtatactatc
      421 acgtactaca ccaacctgga cgaccttgat gggcctttca acgtatcggc cattcatgtg
      481 tgcagatcgg gagcttggac tccaccttgg ttcgacgaca agttcgtcat gttcgttgaa
      541 gcattgggta aggagcatga ggtgatagac tgtccgaaaa gaaaatggga cacaaaaagt
      601 ttgagcctat cacgccagat cgactacctg tttgcataac ctagcatctc cacctggaca
      661 ctttttttgt aacttaggca tttagggtag tgcttgaaag tggctatagt tcagactgct
      721 tccttgaccg caacgagggg caggtataca atagcaatag tgtttttcaa caagtgtaac
      781 tgaagattgt aggaaggtaa aaagacctgc attatgccgg caggctttgg gcttttggtt
      841 ggatgtgcaa acccatcgag gaagcctgaa gctcatccgg actgaacaag aattggacaa
      901 ggaagagtct aaacgatgca tattcaagag atcattagag cgtttatgct gatttttatt
      961 gcagagatgg gggacaaaac ccaaatactc gccatggcgt ttgtcacgcg gtatccggtg
     1021 cggaaggttt taataggcat cggtctgggt gctttcttga accacggatt ggcggtggtt
     1081 ctgggcaact atctttcccg cgtgattccc atcagtacag tccagatgat cgccggtatt
     1141 gcgtttatcg gatttgccct ttggacactg aagcctgaac aggaagaagc ggataaagaa
     1201 gcaaaagttg agtttggtcc cgttgctacg gtggccatgg ctttcttttt aggcgaactt
     1261 ggggacaaga ctcagctcac ggcgattaca ttagcagctg acagcacatc cccaatgacg
     1321 attcttgcgg gaacggtttt aggaatggta gctaccggag ctttggggat ttatatcggg
     1381 aagaagctcg gcgataaaat tccagagctg ggcatcaaag ttctagcatc atgcgttttc
     1441 gcatttttcg gcgttcagaa gctgcttcag acagtgcctg cccagtattt gaacgtctat
     1501 actgtggttc cttccatcgg cttactggga ctagtgatgc tggggatgat ccgttcatta
     1561 atcgtccgga aacgttccgg aatccaatca gagttcctta ccacagcaaa acttctgcgc
     1621 aactactacc ttcatctgca ggaagacctg gaccacatct gtctgggagc cgaatactgc
     1681 ggtacgtgtg caggacggat gtgtgctata ggcaagtcta aagagatcgt taaagccggt
     1741 ctagtcaacg aggaatggca tgacttgcca cagaacggcg gggtatatca caacaacaaa
     1801 cccttcaagc aagaggaagt tttagactgt ttagtggata ccctgtgctt aatcggaacg
     1861 gttcccgatg aaaaagacct ggccaatgca caccttatca ggaatcagat ggaagagatt
     1921 ctcttagggc atcacatcga aagttttgag tccatcaagt cttatatcga agacctaaga
     1981 gaggttgacg ctgtactatc ggaaaaaata gctaagatgg tgcgtgttag aaacaccgct
     2041 aaacaaccag cagaaacggc catggaaagt ccgagacacg tctaacgggg aatactttgg
     2101 gcagctggag ttttcctcaa taattttggc tttcacttaa gtaaaaaacg cagtatacta
     2161 cctaacgtaa atttggaacc tgggcttcta tagagaagtt ccaggttttt ttcttgctcg
     2221 cttgtatcgc gcacttcatg ggttttccgc tagaaaaacc atatggcact cccgcacgtc
     2281 tcagaaattt acaaacaaaa gtgacacttt ggccccacaa caaagggata tggccatagt
     2341 tgctgaagag tttaaacata ctgctatggt gtactgggta acctcagtgt tggttgcttg
     2401 gtttcgcagc agcacgctcc cccagacaga agcaagatcc accgtctcga tgcagaagga
     2461 aacggaccaa cctagtacat atcaaaacag gatcacagaa aaaggacgct acagagattg
     2521 tagctgtcct taactcgata aaaccgaata atcctgtaag gttcggagtt ctaaaggttc
     2581 caccatggat gcctttataa cataaaaaga aaaagaagga ggttgtgtca tggccaaata
     2641 cgtctgttcc gtgtgtggtt tcgagtacga cgaagcaatt ggaataccag aagcgggcat
     2701 tgcgccgggt actaaatggg aagatctgcc agaggactgg gtctgtcctg tgtgcggcgc
     2761 ggagaaatcg gattttgaga tggaaggttc tgtcgactcc acgcctcaaa tagtggaagc
     2821 ggcagtagct gccgaccctg atatgaaaga gctgtcccct ttagaggtca gtgcgctgtg
     2881 ttctaatctt gccagaggat gcgagaagca gtacaagttt gaagaagctg agctgttctc
     2941 tgagctagca gcgttcttta aatcaaaagc agtaccggcc aaagatccag atttcagcca
     3001 actgttcgag cttgtagaaa aagacctcga gcagggtatc ccaaacgcaa atagcgcagc
     3061 agaagcggta agagatcgcg gcgcccttcg ggccttggtg tggaatgaga aggtgacccg
     3121 catcttaaag tcactcctta ctcggtacca gaaagaagga gacgcccttt ttgccaatac
     3181 cggcatttat gtctgtacca tttgcgggtt catctttatc ggggacaagc tgccagacgt
     3241 ttgcccgatc tgcaaggttc ccaactggaa gtttgaaaag gtagagggga ggtaactgca
     3301 atgacaacca agtacgcggt aaggaacata cggctttgca caaaagactg cttgtgtctt
     3361 tttgtctgtc ccaccggagc tactgacacc gagaacagca tcattgatgt tgaaaaatgc
     3421 ataggttgcg gtgattgtgc cgatgcgtgt ccttctgggg ctatctcaat ggtgccgttt
     3481 gagtatccga ctcaacaacc gaaaacagaa acggttgtag gcgcaatgaa tgccctttta
     3541 cggagtaagt cagagcagga gaacatagcc gcctcactgc cgggaatcct ggctaaggca
     3601 atcgaaaaat ccaaccgcat tatggcagaa gatatcctgc gtgaagcagg tttcatgcta
     3661 ccccaaagcg a
//
LOCUS       DAOSDA010000145         3666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16044_length_3666_cov_3.160066, whole genome shotgun sequence.
ACCESSION   DAOSDA010000145 DAOSDA010000000
VERSION     DAOSDA010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16044_length_3666_cov_3.160066"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(376..798)
                     /locus_tag="PLW82_04320"
     CDS             complement(376..798)
                     /locus_tag="PLW82_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46532.1"
                     /translation="MKFIPYSAFIDVYLSLKDYQGSAVVSSFERLKSAYPNIVSISPE
                     TIDEAREKGVMLMELDELEKDDSVVQPVKDLCRYICHNALGGGRDGTVIGMIGTAIDN
                     TPTKLLRASVTSVRKKYPKIYSSQKQLFDELVRASRRW"
     gene            complement(767..1495)
                     /locus_tag="PLW82_04325"
     CDS             complement(767..1495)
                     /locus_tag="PLW82_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ46533.1"
                     /translation="MWNLLGLAHSFNKNFDEAERAFRKASELAPDVALYWTNLSSLLR
                     DKGRLSENSHLTTLARECSHKAIDLQPQNARLYVDVALTYLDESDFSAARQWLDRGLS
                     CEAKTAMDELEALLVLAELHVKAKDYEKVKDVAEQVKNIPEATIPVIAYASKRLTSLG
                     FEMFKANDYFSALAMVEAAQFLYYKKERVGLEVELRSAVDCLNEFSALETDSQVIEPV
                     KRLASMMVSRLLDEDEIHSLQRFY"
     gene            complement(1500..1796)
                     /locus_tag="PLW82_04330"
     CDS             complement(1500..1796)
                     /locus_tag="PLW82_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOJ46534.1"
                     /translation="MTLCNRRRSVITKEEINTAYFRLVVQYTPELNPEKIHLIRNAYE
                     TLTDPKARLNYDAQLASNSEVKQLCDEGHRAMMDQDWVGAINSFKRSSGECSKS"
     gene            complement(2350..3399)
                     /locus_tag="PLW82_04335"
     CDS             complement(2350..3399)
                     /locus_tag="PLW82_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOJ46535.1"
                     /translation="MKGSLEMDIVEVVNQAPVGFQDTGGLELIGPEDTLLDWQQKRED
                     IKRAWSDFLGTPPYGKLGLEFVEHPWEERPDYTGRFVYIKTEPDYYEKHYLLVPKKAQ
                     KKHPAVLVPYYDIDTMVGEKKGGAHFTDTPARFFALELVRRGYVVLAARWFYQGQTEG
                     ETLSHYYEGVKRMEKVYPGFKGLSKIVSDLGYALDYLESLDFVDSERIGVVGHSLGGK
                     MGLYIAAFDGRIAASVLSELGIGMSFSNWDAPWYLGSEVRSEDFSLDHHQLLGLIAPR
                     PVLLIAGDDADTDKSWYYVNCAKSVYDLWGCRERIGLYNHRSGHSPTKEAMEIAYTWL
                     DRYLKPEQGSGRAEK"
     gene            complement(3441..>3666)
                     /locus_tag="PLW82_04340"
     CDS             complement(3441..>3666)
                     /locus_tag="PLW82_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46536.1"
                     /translation="ELWEDEKPKTGTYQQTFELRGGGMKARYVAVKFDTAVWVFIDEF
                     EVFGDPSTKEESTAKVDVYDIDFEEIDFEF"
BASE COUNT          887 a          950 c          792 g         1037 t
ORIGIN      
        1 acgtttattg gcgtagtgtt tgttgttgta tctacacact aaaacagttc tttcattttg
       61 ctgtagtgcg gaaaagaaga acgctctggg gttcctcgat tgtcaaataa ccattgaaaa
      121 ctctcaacac gctttgtgtt tctcaccggt agctagccaa tcccatatag taaaggtcct
      181 caaagtgtct tccgtactat ggaaagtgtg caacaacttt ttcaagtaat aatggctttc
      241 ctttttgtgc ttctatcgga cttcactcga aaagagcacc aaccgagttg attggtgctt
      301 tggtacttta gatatttcta ctgtctactt tacagggagc agttcagaca tctctacgtt
      361 ggaaaaagtt ggggctcacc aacgtcgcga tgcacgaact aactcgtcaa aaagctgctt
      421 ttgtgaactg tagatctttg ggtatttttt tcgtacacta gttactgatg cgcggagaag
      481 ctttgtaggg gtgttgtcaa tagccgtacc gatcatacca atgaccgtcc cgtctctgcc
      541 accacctaaa gcattgtgac agatgtatcg gcagaggtct ttgacaggtt gaactaccga
      601 atcgtctttc tcaagttcgt cgagctccat caacatgaca cccttttccc ttgcttcgtc
      661 aatcgtttct ggactgatgc ttactatgtt cggataagcg cttttcaagc gttcaaaaga
      721 tgaaactaca gcggagcctt gatagtcttt caagctcagg tatacatcaa taaaagcgct
      781 gtagggaatg aatttcatct tcgtcgagaa gcctggagac catcatcgaa gctaagcgct
      841 ttacaggctc gataacttgc gaatcggttt ccaaagcgct gaactcgtta aggcaatcga
      901 ctgcacttct cagttccact tctagtccaa cgcgctcttt cttatagtaa agaaactgtg
      961 cggcctcaac catggccaat gcactgaagt agtcgttggc tttgaacatc tcgaatccca
     1021 aagatgtaag tcttttagac gcataagcga tgactggtat tgttgcttca gggatgtttt
     1081 tgacttgttc tgccacatct ttgacttttt cgtagtcttt tgctttcacg tgtagttccg
     1141 caagaaccaa caaagcttca agctcatcca tcgcagtctt ggcttcacaa gaaagccctc
     1201 gatcgagcca ttgacgggct gcggaaaaat cgctttcatc taagtaggtt aaggccacat
     1261 ctacataaag ccttgcgttc tggggctgca aatctattgc cttgtgagaa cactctctag
     1321 ctaatgtggt tagatggctg ttctcagaca gtctcccttt gtctctgagg agcgaactca
     1381 agttcgtcca gtacaacgcc acatccggtg caagctcgga tgctttccta aacgctcgct
     1441 cagcttcatc gaagttctta ttgaacgaat gggcaagccc cagcaagttc cacactgcat
     1501 caagacttgg agcactctcc agaacttctt ttgaaactgt ttatggcacc aacccagtct
     1561 tgatccatca tagctcggtg gccttcgtca cagagttgct taacctcgct gttggaggca
     1621 agctgcgcat cgtagtttaa cctagctttg ggatctgtca gcgtctcgta cgcgtttctt
     1681 atcagatgaa tcttttctgg gtttaactct ggagtatact gaacgaccaa gcgaaagtaa
     1741 gcggtattga tttcctcttt cgttattaca ctcctcctcc tattgcagag agtcatccat
     1801 gaacgtgaga gtgaaaacag ccaaaatccc aagtacaatg ctctttgtag tgaaaaactc
     1861 agcatgcacg acgatcaagc ccaaaaaatg cattttggtc tacaaatttc tctctgtttc
     1921 tgttttttcc ttctttcatt agatctcttg ttttcacttg acgccaaccc catcaaccta
     1981 caatccttga agtctcttag ctttacctcc ggtaaacatg aaagacgagg taagctcgtc
     2041 taccccgccg aggctatctt tcgggtttaa ccacctacgc taacaaagaa cgtccataaa
     2101 cgttgaaata tcaacgttgt atggacgtcg cttttttatc tggatacact gttaacgcag
     2161 ttcgctcatt taccgtacgc ggaaaaggca agtctcacag cactttttcg ggtgttgctt
     2221 tttactcgca aagagaaaac aaagcaacca gccctctggt ggttttccaa gaagagggct
     2281 gatgcacggt gtcaaacctt tcgactgaac cttcctgaaa gctttaccgc gctttagaaa
     2341 atcaagatgc tacttctctg ctcgaccgct tccctgctcc ggttttaagt accggtcgag
     2401 ccatgtgtag gcgatctcca tggcttcttt tgtgggcgaa tggccactac ggtggttgta
     2461 gagtccgatc cgttccctgc atccccacag atcgtagacg cttttggcgc agttgacgta
     2521 gtaccagctc ttatcggtgt cagcatcgtc tccagcaatg agcaaaaccg gtcttggcgc
     2581 aatgaggcct agaagctggt ggtggtcgag agaaaagtct tcgctacgca cctcggatcc
     2641 gagataccac ggggcatccc agttgctaaa agacatgccg attcccagct cacttaatac
     2701 gctagcggca attctcccgt caaatgccgc gatgtacagg cccatcttgc ccccgaggga
     2761 gtgccccaca acaccgatgc gttcgctgtc cacgaagtca aggctttcta ggtagtccag
     2821 ggcgtacccc agatcgctga cgatcttgct gagtcccttg aaacccggat agactttctc
     2881 catgcgtttg acaccttcgt agtagtggct gagagtttct ccttcagtct gcccctggta
     2941 gaaccaccta gctgcaagaa ctacgtagcc cctccgcacc agttccaatg caaagaacct
     3001 ggctggggtg tcggtgaagt gcgctcctcc cttcttctcc ccgaccatcg tgtcgatgtc
     3061 gtagtaggga acaaggaccg caggatgctt cttctgtgcc tttttcggca ccagaagata
     3121 gtgtttctca tagtagtcgg gctcggtctt gatgtagacg aagcggccgg tgtagtccgg
     3181 tctttcttcc caagggtgct ccacaaactc caggccgagt tttccataag gcggggttcc
     3241 taaaaaatcg ctccaagcac gtttaatgtc ctcccttttc tgctgccaat ccaatagagt
     3301 gtcttccggc cctatcaact ccaaccctcc ggtatcttga aatccaacag gagcctgatt
     3361 taccacttca acgatgtcca tctccaaact ccctttcaat aaaaagaagg tggctcgtgc
     3421 caccttcaat caagctagtc ttagaactcg aaatcgatct cttcaaagtc gatatcgtaa
     3481 acgtcgactt tagcggtgga ttcttctttg gtgctaggat cgccaaagac ctcaaactca
     3541 tcgatgaaga cccaaactgc agtgtcaaac ttgacagcaa cgtatctggc cttcattcct
     3601 ccgcctctca gttcaaaggt ctgttgatat gtgcctgtct tgggcttttc atcttcccaa
     3661 agctca
//
LOCUS       DAOSDA010000146         3653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16121_length_3653_cov_3.301556, whole genome shotgun sequence.
ACCESSION   DAOSDA010000146 DAOSDA010000000
VERSION     DAOSDA010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16121_length_3653_cov_3.301556"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="PLW82_04345"
     CDS             <1..1780
                     /locus_tag="PLW82_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOJ46537.1"
                     /translation="GPNGSGKTTLLNIITGQTEPDDGSVYITSNTTIGYLEQNKDFNL
                     SVPLWDFLSEPLEPLFNCERELFELSQMMPQLAGKELEQALEKYNRLQVWYEVEEGYT
                     ARSRLNMVYNGLGFSETDKHRTVSSFSGGERTRIELARKLLEKPRLLILDEPTNHLDA
                     DTVQWLEEFLKSYPHAFLVVSHDRYFLDEIAQKVLYLESGSIKMYRGNYSDFKKQREQ
                     EEKDLERAYTLQQAYIKKERELIDGARETEKAQRFAKSREKRLEKLEFIERSKSRETL
                     KFQFPFVGRSGNIALRAEGLSFSYSGSRTIVEDASFTIKYGDRVALVGPNGAGKTTLF
                     RLILGELRPDGGHVGFGASVEVSYFSQVHSFKDEENTVLDELIAYKHMTLGRARDHLA
                     KFLFKGDDVLKKVGSLSGGEKSRLILAKMALTSGNFLLLDEPTNHLDIESLETLEEAM
                     LEYPGTILFISHDRYLVSKVAQKIMEIQEGTLTLYPFGYRDYLLAKKNKPSEAQEKTQ
                     KPKEAKQPSASRLSPKRLEREKAKVLEEIHLLEEEKTKAQEGLKDEKNADDWEKLAAL
                     QDEIAQIDSKLSELYEVWLELEEASS"
     gene            2018..3247
                     /locus_tag="PLW82_04350"
     CDS             2018..3247
                     /locus_tag="PLW82_04350"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HOJ46538.1"
                     /translation="MSLEEKVGQMLLLGISDTYVSSTVKQVVSELKAGGIVLFASNLT
                     STAQAVELTTKLQQEALKNRIEVPLLITADEEGGLVSRMPYGMVTMPGNMALGASRNE
                     YLAMLAYYHTGRQLRALGINMNLAPVLDVNNNINNPVIGVRSFGSDPVLVANMGVAAL
                     AGLKNAGVGSVVKHFPGHGDTAIDSHLALPIVRKTKEELEKMELLPFEKALQNGARAV
                     MTAHISFPNLTGGTSVPATLSGEILTGILRASWGYDGIIATDDMLMNAIANHFGFKEA
                     VVMAVKAGADQILIAGNPSKQREAQQALLDAVKRGEISEARIDQSVERIFALKAELGL
                     FPSEMPEYLDPKTEAEEAGKIALEVALGATTRLKGEGRPLSKNEKVLLVAVDNNLQGA
                     GGRNGQKISNYMRLQLN"
     gene            3344..3604
                     /locus_tag="PLW82_04355"
     CDS             3344..3604
                     /locus_tag="PLW82_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46539.1"
                     /translation="MLYLTRNATDEQIELASKITNKPLILVALQNPWAITDSENIKAI
                     FATYGYTEASLEALTMVLAGKEEAMGRLPVDISESLPYGHRN"
BASE COUNT         1071 a          788 c          969 g          825 t
ORIGIN      
        1 tggtccaaac gggtcgggaa agactacgct cttaaatatt atcacaggtc agaccgaacc
       61 cgatgacgga agtgtttaca ttacatcgaa caccaccatc ggttaccttg agcaaaataa
      121 agacttcaat ctaagcgttc ccctttggga cttcctctct gagcctttag aaccgctttt
      181 caactgtgag agggagcttt ttgaactgtc ccaaatgatg ccacagctgg cggggaaaga
      241 actggaacaa gcgttagaga agtacaatag actccaggtc tggtacgagg tggaagaggg
      301 gtatacagcc cgcagtaggt tgaacatggt ctataacggg ttgggatttt cggagaccga
      361 caaacacagg acggtttcca gcttttccgg aggcgaaagg acccgtattg aactggcgcg
      421 aaagctcctg gaaaaaccca ggctgttgat tttagacgaa cctacgaacc atcttgatgc
      481 agatactgta cagtggctgg aggagtttct taagtcctat ccccacgctt ttttggttgt
      541 ttcgcacgat agatactttc tcgatgagat agcacagaag gtgctgtacc ttgaaagcgg
      601 ttctatcaag atgtacagag ggaactattc cgacttcaaa aagcaacgcg aacaggaaga
      661 aaaggacttg gaaagagcct atactctgca gcaggcctat attaagaagg agagagagct
      721 gattgacggt gctagggaaa ccgaaaaggc tcagaggttt gccaagtcca gggaaaaaag
      781 gcttgaaaag ctggagttta tcgaaaggtc taagtcccgc gaaaccttga agtttcagtt
      841 tccttttgtg ggaaggtcgg gaaacatcgc actgagagca gagggtttgt ctttcagcta
      901 ctcagggagc agaacgattg ttgaagatgc ttcgttcact atcaaatatg gagacagagt
      961 ggctcttgtc ggaccaaacg gtgctgggaa gaccacccta ttcagattga ttctcggaga
     1021 actgagacca gacggtggcc atgtgggctt tggtgcaagc gtagaggtca gctacttttc
     1081 tcaggttcac agctttaaag atgaagaaaa caccgtgcta gatgaactca tcgcatacaa
     1141 acacatgacc ctaggacggg ctagggacca ccttgctaag ttcctcttta aaggtgatga
     1201 tgtgttgaaa aaggtgggga gcctctcagg cggcgaaaaa agcagactga ttttggcaaa
     1261 aatggctctt acaagtggga acttcctgct cttggacgag cccacgaacc atctggatat
     1321 tgagtctttg gagacgctag aagaggccat gttggaatac cccggaacaa ttctgttcat
     1381 cagccacgat agatatctgg tgtctaaagt tgctcagaag atcatggaga ttcaggaggg
     1441 taccttaacc ctttaccctt ttgggtaccg ggattatctt ttagcgaaaa agaacaagcc
     1501 ttctgaagct caagaaaaga cccaaaagcc caaagaagca aagcagccgt ctgcttctcg
     1561 actaagtccg aagagattgg agcgggaaaa ggcaaaagtt ttagaagaga tacatctcct
     1621 ggaagaagag aaaacgaaag cccaagaagg ccttaaggac gagaaaaacg ccgatgattg
     1681 ggagaaactt gctgctctcc aagatgaaat tgctcagata gacagtaagc tttccgagct
     1741 ttatgaggtt tggcttgagc tggaagaagc aagctcttag aagcgtacct ttatggtagg
     1801 caccaacgca gaaaacgcaa gtttaaccta acgttgagct tgtgagatta tagagacctt
     1861 aaaaaaaggg ggtctagttg tactacccag ttgggtattg gcaacgcatt atatgattta
     1921 tgtgaaaaaa ggtattgtgt ctttgctaat agctttttgc ctcgtgctga ccgtcagtgc
     1981 ggagctccca gtccaatatc agaaagtgtt ggaatcgatg tcccttgagg agaaggtcgg
     2041 acagatgctg ctactaggaa tttccgacac ttatgtcagc agcaccgtga agcaggtagt
     2101 gtccgagctg aaggcagggg gtattgtgct gtttgcgtct aacctgacat ccactgccca
     2161 ggctgtggaa cttacaacga aactccagca ggaagcactg aaaaacagga tcgaggtacc
     2221 tcttctcatc acggcggatg aagaaggagg tctagtctca cgcatgcctt atggcatggt
     2281 tacaatgcct gggaacatgg ctctaggtgc ttcaagaaac gaatacctgg ccatgcttgc
     2341 ctattaccac acaggaagac agctcagggc attggggatc aacatgaatc ttgcccctgt
     2401 tctggacgta aataacaaca taaacaaccc ggtgatcggc gtaaggtcct ttggatccga
     2461 tcctgtgttg gtggcaaaca tgggagttgc agcgcttgcc gggctgaaaa acgccggtgt
     2521 aggaagcgtt gttaagcatt ttcccggtca tggcgatacc gcaatcgaca gccatctagc
     2581 tctgccgatc gtcaggaaaa ccaaggaaga acttgaaaaa atggagcttc tgcctttcga
     2641 aaaagcgttg caaaacggtg ccagagcggt tatgactgcg cacatttcct ttcccaacct
     2701 tacaggggga acctctgtcc ctgcaaccct ttctggggag attcttaccg gaatactaag
     2761 agcaagttgg ggatacgacg gcattattgc aaccgatgat atgctgatga acgccatagc
     2821 caaccacttt ggatttaagg aagctgtagt tatggccgtg aaagccggtg cagatcaaat
     2881 tctcattgcg ggaaacccca gcaagcagag agaggcacag caggcattgc tggatgcggt
     2941 gaaacgaggc gagatttccg aggcccgaat cgaccaatca gtggaaagga tatttgctct
     3001 aaaagccgaa cttggtcttt tccccagtga gatgccggag tacctagacc ccaaaaccga
     3061 agcagaagag gcagggaaga tcgctttaga ggtggctctt ggagcaacaa ccaggttaaa
     3121 gggtgaaggg agacccctaa gtaagaatga gaaagtactc cttgtagcgg tagacaacaa
     3181 cttacagggg gcaggaggca gaaatggcca gaagatcagc aattatatgc gcttgcagct
     3241 gaattagaag gggtttgata gtgttgaaac tgtcagcatc agctacaggc cgactctggc
     3301 agagcaggca gaagtcgtaa aaaaagctca gcaagccgat gtggtgcttt accttacgcg
     3361 aaacgccaca gacgaacaga ttgagctggc tagtaaaata acgaacaagc cacttatttt
     3421 agttgctctg cagaacccat gggctattac agactcagaa aacattaagg ctatctttgc
     3481 aacctatggc tacacagaag cttcactaga agcactgacc atggttctag caggaaaaga
     3541 ggaagcgatg gggcggctcc cggtggatat ctccgagagt ctgccctatg gacaccgcaa
     3601 ttagcagtag aacgcagtgt tgagttagtc cagaaagcac tgcacgcata tta
//
LOCUS       DAOSDA010000147         3626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16282_length_3626_cov_3.649398, whole genome shotgun sequence.
ACCESSION   DAOSDA010000147 DAOSDA010000000
VERSION     DAOSDA010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16282_length_3626_cov_3.649398"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLW82_04360"
     CDS             complement(<1..516)
                     /locus_tag="PLW82_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOJ46540.1"
                     /translation="MLLKLFLTFLKIGAFTFGGGYAMLPLFQRDIIERNHWLTQSDFL
                     DIVATAEMTPGPIAINTATFVGYKLSGFWGSLVSTLAIVLPSFISITLLAFILNKLKD
                     NKDAQSGIKGMRPAVTAMIITAALSLAPQSIVRPLDIILFAGALILSWKNWLNPIVIL
                     LGSGVISLLFGL"
     gene            complement(516..1079)
                     /locus_tag="PLW82_04365"
     CDS             complement(516..1079)
                     /locus_tag="PLW82_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOJ46541.1"
                     /translation="MLWSLFINCFKVGALTIGGGYAMLPVMKEVFVTKKKWVSEEDFV
                     DMMAVIQTVPGAIAVNSSIYLGRKIAGIPGAIAATIGSVLPSFLIILLIASTLTSWYQ
                     NPYIANFFAGVRPAVIALILVAGVKLGKGILHSKIQIALTLLFALLALVLKFHPVVLL
                     VMGAAIGILLSRISEKSNASEKKGRDQ"
     gene            1439..2065
                     /locus_tag="PLW82_04370"
     CDS             1439..2065
                     /locus_tag="PLW82_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46542.1"
                     /translation="MKKTLTVLLVLALAVVTFAAPQLIYEANFNKTDGKNVDWVPVNG
                     DWDIDGEYMVQYDLNDGNTNIWQELAQYGDGLFIYEYKITYDVAGGQWAPAAGLHFMA
                     SDGDAPQRGNSYLVFQDLNEMQLYRCEGGGIVAVKLTGMDPAIEGQTNVIRVEFETIT
                     GRIAVFLNDRLVIEWFDDYPILDGDFISLRTNQTAVRYDYVKVWYQKM"
     gene            2252..2788
                     /locus_tag="PLW82_04375"
     CDS             2252..2788
                     /locus_tag="PLW82_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOJ46543.1"
                     /translation="MTKKSNLRANLATAVLISIASILHVFEGLLPPLPVPGAKLGLAN
                     IASLIGLRMLGFGSAIVISLTRCFIGALFGSGLFGPSFYLSLAGSLSSAIAMGLASKI
                     KGIGNKNIVVSVVGALTHSTAQIVVASFLLSHQGIWWYLPYLLGLSSITGVFIGIASD
                     KLLVYLPRAIGLEPQSKS"
     gene            2816..3202
                     /locus_tag="PLW82_04380"
     CDS             2816..3202
                     /locus_tag="PLW82_04380"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOJ46544.1"
                     /translation="MQQKKKQDWILILAFLALGAGLIFGGKFFMGLREAEEGLKANIY
                     ELTSDGRKLLESFTLKEGEQAVYEFHGKLGVTVVEIDGLKARVRSSPCPDQVCVQFGW
                     LQFHDDFAACLPNSMIVVLEGDGVGY"
     gene            3313..>3626
                     /locus_tag="PLW82_04385"
     CDS             3313..>3626
                     /locus_tag="PLW82_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46545.1"
                     /translation="MANVIAAPIGFGWSITEKDSSIQIDVGFKVEIDRKSGALTALGA
                     HKVDGPFRIKKGESWLGPNDLELVSATWNNEDSSLTLTYKDETALTLFLRKTRSPAGS
                     PA"
BASE COUNT          981 a          793 c          893 g          959 t
ORIGIN      
        1 aaggccaaaa agtaagctga taaccccgct gccgaggaga atcactattg ggtttaacca
       61 gtttttccac gacagaatca gagctccagc gaaaaggata atgtctaaag gacgcacaat
      121 ggattgaggg gctaaggata gagctgcagt gataatcatt gcagtgaccg ccggacgcat
      181 tcccttaata ccactttgtg cgtctttgtt atccttaagc ttgttaagga tgaacgcaag
      241 gagagtaatg gagataaacg aaggcagaac aattgccaat gtcgacacta aagatcccca
      301 gaaaccggaa agcttgtagc cgacaaaagt ggcagtgtta atggcgatcg gtcctggagt
      361 catttcagcc gtagcgacga tgtcaaggaa gtcgctctgt gttagccaat ggtttctctc
      421 gatgatatcc ctctgaaaga gaggcagcat ggcgtatcct ccgccgaatg taaatgcacc
      481 gattttcagg aacgttaaga aaagcttgag cagcattact ggtcacgccc tttcttttca
      541 gaggcattgg acttttcgct tatccgtgag aggaggatac cgatggcagc gcccataaca
      601 agaagaacca ctggatgaaa cttcagtact aaggccaaaa gggcaaaaag cagggttagt
      661 gcgatttgga tttttgagtg caggatacct ttgcctaatt tcacccctgc aacgagaatg
      721 agggcaataa ctgcaggtct tacccctgcg aagaagttcg caatgtaggg gttctgatac
      781 caactggtaa gggttgatgc gataagcaga atgatcaaga aagaaggcag taccgaaccg
      841 attgttgcgg caatagcgcc tggtatgcca gcaatctttc ttcccagata gatgctggag
      901 ttgacggcga ttgcgcctgg gaccgtttga atgactgcca tcatatcaac gaagtcctct
      961 tcggaaaccc attttttctt ggtgacgaat acttctttca tcacaggcag catggcatat
     1021 ccaccgccta tggttaaagc ccctacctta aagcaattga taaataaact ccaaagcatg
     1081 tcttcacctc tagataaatg atagactgga tagacttgtg ctcagtctag ttggaaccaa
     1141 aagtgtttgg acttactaat tataacactt ttacttccct taagtagtct ttcccaggca
     1201 gaacctcttc ggtctgaaaa ttaagacatc aaggcttttt gggctttacg atagtcgttt
     1261 tcccaccata aaggtctgtg ttatagaaca aacgaaacct tgataattct ggaaaaggaa
     1321 atttttctac tcaatgaagg aattgcatga gattcataga agaagaaaat gtagatgtac
     1381 ccatcgtata gacggacatc tcgtggggaa caattacaat cagagggggg tttttcctgt
     1441 gaaaaaaacc ttaacagttt tgctggtgtt ggcactggca gttgtaacat ttgcagctcc
     1501 acagttgatc tacgaggcca acttcaacaa gacagatggc aagaatgttg actgggtacc
     1561 ggtcaatggc gattgggata ttgacgggga gtatatggtc caatacgatc tgaacgatgg
     1621 aaacaccaac atttggcagg agctggcaca atacggcgat ggacttttta tctatgaata
     1681 caaaatcact tacgatgtag ccggcggaca atgggcacct gcagcaggtt tgcacttcat
     1741 ggcttctgac ggtgatgcac cacaacgtgg caacagctac ctggtattcc aagacctcaa
     1801 tgaaatgcag ctctaccgct gcgagggtgg aggaatcgta gcagttaaac tcactggtat
     1861 ggatcccgct atcgaaggtc aaacaaatgt cattcgtgta gagtttgaga ccatcaccgg
     1921 ccgtatcgcc gtattcctca atgacaggct ggtcatcgag tggttcgacg actacccaat
     1981 cctggacggt gacttcatct ccttaagaac taaccaaact gcagtacgtt atgactacgt
     2041 aaaagtttgg taccaaaaaa tgtaattgag ctttaggcct tgcgaaaagc aaggcctttt
     2101 tttgtgtcca gcccccttag cttgctttta gctttgccca atgatatgat ttattttggt
     2161 acaagcccga agagggcttt tgtgtttgtt ttgacttcta tgctgataaa tcgcagcttg
     2221 agcgtcaggc taagctagac aggtgtgaat catgaccaag aaatctaatc taagagctaa
     2281 tctagcaacg gcagttctaa tcagcatcgc cagcattctt cacgtgtttg aaggcctgct
     2341 gccaccgctt cccgttccag gcgcgaaact gggactagcc aatatcgcat cgctcatcgg
     2401 gttacggatg ctgggctttg gctctgctat agtaattagc cttactcgat gcttcatagg
     2461 tgcccttttt ggaagtggtc tttttggacc ttccttctac ctcagtcttg ccggatccct
     2521 cagcagtgcg attgccatgg gacttgcatc gaagataaag ggtattggga acaagaatat
     2581 agtcgtcagc gtggttggag cgctcactca cagcaccgct cagattgttg ttgcatcatt
     2641 tcttctcagt caccaaggca tttggtggta tctcccgtat cttttgggac tttcatcaat
     2701 tacaggcgta ttcatcggca tagcctccga caagctgctg gtctacctgc ctcgagcgat
     2761 agggttggag ccacagagca aaagctgaag gccgaatacc gggagtggtt tctttatgca
     2821 gcagaaaaaa aagcaggact ggattctcat tttggctttt cttgccttgg gcgctggcct
     2881 gatttttggt gggaagttct ttatgggact tcgagaagct gaagaaggtc tcaaagccaa
     2941 catctacgag ctgacttctg atgggcgaaa actcctcgag tcgtttaccc ttaaagaagg
     3001 tgaacaggct gtatacgagt tccacggcaa gcttggtgtg actgtggtag agatcgacgg
     3061 cctcaaggcc agagtcagat cttcgccttg tccagatcaa gtttgcgtcc agttcgggtg
     3121 gttgcagttt cacgatgatt ttgccgcttg cctccccaat tcgatgatcg tggttctcga
     3181 aggtgatggc gtaggatatt aatcttatat agaacgattt aagtttagag acagtattgt
     3241 ttctaaactt tttttgttgc aggagagtct tcttaggcta cgaattatat ccatgctggg
     3301 aggtagataa ctatggcgaa cgtcatcgca gctccgatag gtttcggctg gtcaattaca
     3361 gaaaaagaca gctctattca aattgatgtt ggctttaaag tggagatcga tcgaaagagt
     3421 ggggcattga ctgctctcgg cgcccacaaa gttgacggac cctttaggat caaaaagggc
     3481 gaaagctggt tgggaccgaa cgacttggag ctggtaagtg ccacatggaa caacgaagac
     3541 tcatcgctca ccttgactta caaagatgaa accgctttga cgcttttttt gcgaaaaacc
     3601 agatcaccgg caggctcacc ggcgtt
//
LOCUS       DAOSDA010000148         3618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16334_length_3618_cov_3.406399, whole genome shotgun sequence.
ACCESSION   DAOSDA010000148 DAOSDA010000000
VERSION     DAOSDA010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16334_length_3618_cov_3.406399"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /gene="glyA"
                     /locus_tag="PLW82_04390"
     CDS             <1..1005
                     /gene="glyA"
                     /locus_tag="PLW82_04390"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOJ46546.1"
                     /translation="VNVQPHSGAQANTAVYFAVLKPGDTVLGMDLTHGGHLTHGHPIN
                     FSGTYFNFVPYGVSRETETIDYNEVEMLALQHKPKLIVAGASAYPRVIDFAALKAIAD
                     KAGALLMVDMAHIAGLVAAGLHPSPVPYADFVTTTTHKTLRGPRGGMILCKEQYAKDI
                     DRAVFPGIQGGPLMHVIAAKAVAFKEALDPSFKKYQAAILDNAKALAEGLIEEGWRLV
                     SGGTDNHLMLVDVKAKGMTGKDAEHILDSVGITVNKNTIPFDTEKPFVTSGIRLGTPA
                     LTTRGMGKAEMKKIASIIDIALVEKDFEKAKSMVQELTEAFPLYKEIRALYKGDSVNA
                     "
     gene            998..2272
                     /gene="serS"
                     /locus_tag="PLW82_04395"
     CDS             998..2272
                     /gene="serS"
                     /locus_tag="PLW82_04395"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOJ46547.1"
                     /translation="MHDLKEIRDNPEKFRTALKRRGEDVTVIDTIIDLDEERRKIIAE
                     VEVLKSQRNSESQKVGELKKKGEDASAIIAAMKELGDRIKELDDEQRVIEEKIQDVLL
                     GIPNVPHPSIPDGKDDTENVTVSFWGEPTKFDFEPKAHWDIGTDLGIMDFDRAGKITG
                     SRFTILKKEGAKLERALISFMIDVHTSEHGYTEVYPPFMVNTASMTGTGQLPKFAEDA
                     FKVDKTDYWLVPTAEVPVTNMYRDEILDVADLPLYHVAYTACFRAEAGSAGRDTRGLI
                     RQHQFDKVELVKFVLPESSYDELEKLTANAARILELLGLPYRKTMMCTGDVGFSAAKK
                     YDLEVWMPSYNNYVEISSCSNFEAFQARRANIRFRREKGAKPEFVHTLNGSGLAIGRT
                     AAAILENFQTADGSVIIPEVLRPYMGIDKISR"
     gene            2328..3005
                     /locus_tag="PLW82_04400"
     CDS             2328..3005
                     /locus_tag="PLW82_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ46548.1"
                     /translation="MGKEIVIDVRGLYFGYLKEPILQNLYFQARQGERIAIVGANGSG
                     KTTFLKLLLGHLKPWEGEIKILDREIASTQDRVFSRKHIAYVPQERIVGKMPISVYDA
                     VLLGRYAVGFAGIKKPTQKDRELALEAIEAVGLKDFVRADVSTLSGGQLQRMSIARGL
                     VRKAPIMFLDEPIAHLDKLGKEDIPLLLENIHLEKQFCMIVITHENISMDFDRVFHLE
                     AGLLKEI"
     gene            3010..>3618
                     /locus_tag="PLW82_04405"
     CDS             3010..>3618
                     /locus_tag="PLW82_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOJ46549.1"
                     /translation="MSIAAYFHYPIFQRAFLACVLAGGLVSLVGVVIVVLNLTTIRFS
                     LMHAALLGSAVAMVLEFPSVWGALIAIALTAWFVGPLSDKLKVDTGLSGALFMTGTLA
                     LAFFLFYQAGVPAMEAFDVFSGNILALTRADVVSLCAVSLVILILAICFYRELQLVLY
                     NEELAQTLGIHSKLVRNVMLLISGMAIGVVMRLVGALLVDAII"
BASE COUNT          992 a          799 c          956 g          871 t
ORIGIN      
        1 gtcaatgtgc agcctcattc aggagctcag gctaacaccg cagtgtactt cgcagtactg
       61 aaacctggtg atacggtgct gggcatggac ttgactcacg gagggcacct gacccacgga
      121 catccgatca acttctccgg tacctacttt aacttcgtgc cttatggtgt aagcagagag
      181 actgagacca tcgattacaa cgaagtggag atgctggcgc tgcagcacaa accgaaactg
      241 atcgtggcag gggctagtgc ttaccccaga gtcatcgact ttgccgcact gaaagctata
      301 gctgacaaag caggtgcgct tttaatggtc gatatggcgc atatcgccgg acttgtggca
      361 gcgggactgc atccctctcc tgtaccgtat gcggattttg tcaccaccac aactcataag
      421 actctaaggg ggccaagagg tggcatgatc ctctgtaaag agcaatacgc caaagatatc
      481 gacagagctg ttttccccgg catccagggt ggacctttga tgcacgttat cgctgcaaag
      541 gccgtagctt ttaaagaagc tttagatcca agctttaaga agtatcaagc agctatcctg
      601 gataacgcca aagcattggc agagggactg atcgaggaag gttggcggct ggtatctggt
      661 ggtacagaca accacttgat gcttgttgac gtaaaagcta aaggtatgac tggtaaagat
      721 gcagaacata tccttgacag tgttggcatc accgtcaaca agaacacaat tcccttcgat
      781 acagaaaagc cgtttgtcac aagcggcatt agactaggga ctcctgcatt gaccacgaga
      841 ggtatgggga aagctgaaat gaagaagatc gcctcgatca ttgacatcgc tttagtggag
      901 aaggatttcg aaaaagccaa gtcaatggtt caagaactta cagaagcttt tccgttgtat
      961 aaagagatac gtgcacttta taagggggac agcgtaaatg catgatctta aagagattag
     1021 ggacaacccg gagaagttta gaactgctct gaaacgaaga ggcgaagacg ttaccgtcat
     1081 cgacaccata attgatttag atgaagaaag aagaaagatc attgccgaag tagaagtctt
     1141 gaaaagccag agaaacagcg aaagccaaaa agttggagag ttgaagaaaa aaggtgagga
     1201 tgcatctgcg atcattgctg ctatgaagga gcttggcgac aggatcaaag agctcgacga
     1261 tgagcaaaga gtgatcgaag aaaagatcca agatgttctc ctcggtattc ctaacgtacc
     1321 ccatccaagc attcccgatg gcaaagacga caccgaaaac gtgaccgtaa gcttttgggg
     1381 cgagccaact aagttcgact tcgaacctaa agcccactgg gatatcggta cagatttggg
     1441 aattatggac ttcgacagag ccggcaagat cacaggttcg cgattcacga tcttgaagaa
     1501 ggaaggtgca aagctggaaa gagcactgat cagcttcatg atcgatgttc acacatcaga
     1561 acacggctat acagaggtct atccaccgtt tatggtcaac actgccagca tgaccggtac
     1621 aggtcagctg ccgaaatttg cagaggacgc atttaaggtc gacaagaccg actactggct
     1681 cgttcctact gcagaagtac cagtgacaaa catgtatcga gacgagattc ttgacgtagc
     1741 ggatctgccg ctctatcacg tagcttatac cgcctgcttt agagcagaag ctggatcggc
     1801 cggcagagac acaagaggcc taattcgcca gcatcagttt gacaaagtag agctggtgaa
     1861 gttcgttctt ccggaaagct cttacgatga gttggagaag ctcactgcta acgcagcgag
     1921 gatcctagag ctcttgggcc ttccataccg caaaaccatg atgtgtacag gcgacgtagg
     1981 attttctgca gctaagaagt acgaccttga ggtttggatg ccaagttaca acaactacgt
     2041 agagatctcg tcctgttcga acttcgaagc cttccaggct cgcagagcta acattcgctt
     2101 cagaagagag aagggcgcaa aaccagagtt cgtccacact ctcaacggtt ccggtctggc
     2161 aattggcaga actgcagcgg ctatcttgga gaacttccaa acagcagacg gttctgtaat
     2221 tatacctgaa gtgctcagac cctacatggg tatagacaaa atttcaagat aaaacaaaga
     2281 cccactgaaa agtgggtcaa tctttacata ggaagggggt ttttgcgatg ggtaaagaga
     2341 tagtgatcga tgtcagaggc ctgtacttcg gctatctgaa agagcccatt ttgcaaaacc
     2401 tctacttcca agcacgccag ggtgagagaa ttgccatagt gggagctaac gggagcggga
     2461 aaacgacgtt cttgaagctt ctactaggac acctcaaacc ttgggaaggc gagattaaga
     2521 tcttggacag agaaatcgcg tcaacccaag accgggtttt ttccagaaag catatcgctt
     2581 atgtaccgca ggagcgcatc gtgggtaaga tgcccatttc tgtttacgat gcagtgcttt
     2641 tgggtaggta tgctgtaggt tttgcgggta taaaaaaacc aacccagaaa gaccgagagt
     2701 tggccttgga agctatagaa gcggtaggat tgaaggactt tgttagggca gacgtttcca
     2761 ctctttccgg aggtcagcta cagaggatgt ctattgctag aggccttgta cgtaaagcgc
     2821 cgatcatgtt tctcgacgag cccatagccc acttggataa gctgggaaaa gaagatattc
     2881 cgctgctctt ggagaacatc cacttagaga agcagttctg catgatcgtg attacccatg
     2941 aaaacatcag catggatttc gacagggtct ttcaccttga agcaggactg ttaaaggaga
     3001 tctagggcta tgagtatagc ggcttacttc cactatccca tatttcaaag ggcttttttg
     3061 gcctgcgttt tggcaggagg attggtttct ctggttggcg tagtgatcgt agttttgaac
     3121 cttacgacca ttcgcttctc tctgatgcat gcagcgcttt taggttctgc agtggcaatg
     3181 gttctagagt tcccctcggt atggggagcc ctgatagcga ttgcccttac tgcgtggttt
     3241 gtggggcctc tgtctgacaa gttaaaggtg gacaccggcc ttagtggagc gttgtttatg
     3301 accggcacat tagcactggc gtttttcttg ttttaccaag cgggagtgcc ggcgatggaa
     3361 gcgttcgacg tcttctctgg aaacatcctg gccttaacga gggcagatgt agtttcgcta
     3421 tgtgccgttt cgcttgtgat cttgattctg gcaatctgct tttacaggga gctgcagctt
     3481 gtactctaca atgaggaact agcacagact ctgggtattc atagcaagct ggtaaggaac
     3541 gttatgctgt tgatctctgg gatggcaata ggcgtcgtta tgcgccttgt aggagcgctg
     3601 cttgtggatg ccatcatt
//
LOCUS       DAOSDA010000149         3602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16431_length_3602_cov_3.024528, whole genome shotgun sequence.
ACCESSION   DAOSDA010000149 DAOSDA010000000
VERSION     DAOSDA010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16431_length_3602_cov_3.024528"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /gene="dnaK"
                     /locus_tag="PLW82_04410"
     CDS             <1..1605
                     /gene="dnaK"
                     /locus_tag="PLW82_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOJ46550.1"
                     /translation="TIGSIKRHMGTDHKVKIRDKEYTPQQISAFILSKLKKDAENYLG
                     EEVTQAVITVPAYFQDAQRQATKDAGRIAGLEVLRIINEPTAAAMAYGLDKIEKHETI
                     LVYDLGGGTFDVSILDIGDGVFEVKATSGNTRLGGDDFDKRIIDYVVKEFKAKEGVDL
                     SQDKQAMQRLKEAAENAKKELSNVVSTNINLPYVTLTSSGPKHLDVTLTRARFDELTK
                     DLVEATLGPTRTALSDAGLKPEDIDRILLVGGSTRIPAVQEAIKKFFGKEPHKGINPD
                     ECVAIGAAIQAGVLGGEVHDIVLLDVTPLSLGIEVEGGLFNKVIERNTTIPTKASRIY
                     TTAADFQTAVTIHVLQGERTMAKDNISLGMFNLEGIPAARRGVPQIEVTFDIDANGIV
                     NVSAKDKATGRENKITVTGSKSLSEAEIERMIKDAEAHRAEDERRKQEIEEKNNAQNL
                     LYSVDHTLEDLKDKITEDQKKKIEDAKSELKTALEGDNMDEIRTKKQALEKILSDISS
                     EIYAKTKDAQDGTQQTKQGDDEIIVE"
     gene            1657..2793
                     /gene="dnaJ"
                     /locus_tag="PLW82_04415"
     CDS             1657..2793
                     /gene="dnaJ"
                     /locus_tag="PLW82_04415"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOJ46551.1"
                     /translation="MSASKRDYYEILGVPRGASKEEIKQAYRKLAKKYHPDLNKDNPN
                     AEEMFKEINEAYQVLSDDEARARYDQFGHSAFDQGAGGPGSGGFGDIFGEFDFSDIFD
                     LFGSFTGGTRRRGPVRGEDISTTVDITFEEAAFGTTKTVKYRRLTECSRCKGSGAEPG
                     TSVDVCPQCHGSGQVQEERNTIFGRTVSVRPCPTCGGEGRSIKTPCTNCRGDGRAVES
                     EQISINIPPGVDTGNRVIARGKGAKGVRGGDYGDLIINIRVRPHEIFKRDGANLLLDV
                     PITFAQAALGDEIEIPTLEGMETYKIPAGVQSGETRRIRGKGLNYLNSNNRGDLIITI
                     VVQTPKKLSPEERELFEKLKRIQTVESNSKSAFDKFRDFLRGKK"
     gene            2807..>3602
                     /gene="prmA"
                     /locus_tag="PLW82_04420"
     CDS             2807..>3602
                     /gene="prmA"
                     /locus_tag="PLW82_04420"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOJ46552.1"
                     /translation="MKWLEITAHFTDEIKEPLLVHLLDLGIQGVIEEESAYRFYLNED
                     TDPQTIFTGQFGEVPFSAEVVDDANWAENWKQFWHANKIGKFVVVPSWESYEKQGAEI
                     VLNLDPGMAFGTGAHETTALVLEFLGEVPVVPETVYDIGCGSGILAIAAAKLGAEVYA
                     FDTDPLAVDATLKNAEKNHVEGLIKVFGGNLLEDESLFPDKVPDLVLTNIIAEVIVEF
                     VPLLKEKLGTFTWIVSGIISERLPMVLGALEEHGFAVTKRKDRGEWV"
BASE COUNT         1036 a          855 c          968 g          743 t
ORIGIN      
        1 accattggtt ccattaagag gcatatgggc actgaccaca aagtaaaaat cagagacaaa
       61 gagtacacac cgcagcagat atccgcgttt atcttgagca agctgaagaa agacgctgag
      121 aactacttag gtgaagaagt tacccaagcg gttattaccg ttccagctta cttccaggat
      181 gcccaaaggc aggcaactaa agatgccggc cgcattgcag ggctagaggt tctcagaatc
      241 atcaacgaac ctactgcagc cgcgatggcc tacggtttgg ataagattga aaaacacgaa
      301 accatcttgg tttacgacct cggcggcgga acctttgacg tttccattct ggatatcggc
      361 gatggcgtct ttgaggttaa agctaccagc ggtaacacca ggctcggtgg agatgacttt
      421 gataaacgca tcatcgatta cgtagtcaaa gagtttaaag ccaaagaagg cgtggacctg
      481 tctcaagata agcaggcaat gcagaggctc aaagaagctg cggaaaatgc caagaaggag
      541 ctttccaacg ttgtctctac caatatcaac ctaccttacg tcactttgac aagttccgga
      601 cccaaacacc tcgatgtaac cctgactcga gccaggttcg acgagctgac caaggaccta
      661 gttgaagcaa cccttgggcc aactagaacg gctctgtcag atgccggact gaaaccggaa
      721 gatatcgaca gaattcttct tgtgggcggc tcaaccagaa ttcctgcagt tcaagaagcc
      781 atcaagaagt ttttcggcaa agaacctcac aaaggtatca accccgatga gtgcgtggct
      841 atcggcgctg ccattcaggc aggtgtactt ggcggggaag ttcacgatat cgttctcttg
      901 gacgtaacgc cgctgtcttt gggtattgag gtagaaggcg gtctgtttaa taaagttatt
      961 gagagaaaca ccaccattcc tactaaggct tctaggatct acaccactgc agcagacttc
     1021 caaactgccg ttactattca cgttctccaa ggcgagagga ccatggcaaa agacaacatc
     1081 agcctgggca tgttcaacct cgagggtatt ccagctgcaa gacgcggcgt tcctcagatt
     1141 gaggtaacct tcgacatcga tgccaacggc atcgtcaacg tctccgccaa agacaaagct
     1201 acagggcgcg agaacaaaat caccgttacc ggttccaagt cactgtcaga ggcggagatc
     1261 gagcgcatga ttaaagacgc tgaagctcat agagccgaag acgaacgcag gaagcaggaa
     1321 atcgaagaga aaaacaatgc acagaacctg ctctattctg tagatcatac cttagaggat
     1381 ctgaaagaca agatcactga agaccagaag aagaagatcg aagacgccaa gagcgagctg
     1441 aaaaccgctt tagaaggcga caatatggat gagatcagaa cgaagaagca agccttagag
     1501 aagattctct ctgatataag ttccgagatc tacgccaaga ccaaagacgc acaagatgga
     1561 acgcagcaaa cgaaacaagg tgacgacgag ataatcgttg aatagattat ttggggcggc
     1621 tagccgcccc attttaggtt atgggaggtg atagggttga gtgcgagtaa aagagactac
     1681 tacgaaatcc taggtgttcc cagaggggct agcaaggaag agatcaagca agcctaccgg
     1741 aaactagcca aaaagtacca cccggacttg aacaaagaca atcccaatgc ggaagagatg
     1801 ttcaaggaaa tcaacgaagc ctatcaagta ctcagcgatg acgaagcgag agcccgctac
     1861 gaccagttcg gccactctgc ttttgatcag ggagcaggcg gacctgggtc cggcggattt
     1921 ggcgatatat ttggcgagtt cgacttctcc gacatattcg acctgttcgg cagctttaca
     1981 ggcggcacac ggcggagggg ccctgtaagg ggtgaagata tctccaccac tgtagatatt
     2041 acctttgagg aagccgcctt cggcaccaca aagaccgtga aatacagacg cttaacagag
     2101 tgcagccgct gtaaaggttc cggcgctgag ccgggcacat ctgtcgacgt ctgtcctcag
     2161 tgccacggtt ccggacaggt tcaggaggag cgcaacacca ttttcggccg cactgtgtcg
     2221 gtgagaccct gtcctacctg cggcggggaa ggccgctcga ttaagacgcc ctgcactaac
     2281 tgccgcggcg acggcagggc tgttgagagc gaacagatca gcatcaacat tccgcctggg
     2341 gtagatactg gcaacagagt aatcgccaga ggcaagggcg caaagggagt aagaggcgga
     2401 gattacggag atctcatcat aaacatcaga gtgcgtccgc acgagatctt caaacgcgat
     2461 ggcgcaaacc tcctattaga tgtgcccatc acctttgcac aagctgctct aggcgatgag
     2521 atcgaaatac cgactttaga aggcatggag acctacaaga ttcctgccgg agttcagtcc
     2581 ggagaaacca ggaggattag ggggaagggg cttaactacc tcaactccaa caatagggga
     2641 gatctgatca tcacgatcgt tgtgcagacc ccgaagaagc tttccccaga ggaacgggag
     2701 cttttcgaaa agctcaagcg catccagacc gtagagagca actcaaagag cgcatttgac
     2761 aagtttagag acttcctgag gggcaagaaa taaggagcgg aatgccatga aatggttgga
     2821 gattacagcg cattttactg acgagatcaa ggaacctctt ctcgttcatc tcctggattt
     2881 gggaatccag ggagtaattg aagaagagtc tgcctacaga ttctacctta acgaagatac
     2941 tgatccccaa acaattttta caggtcagtt tggggaagtg cctttctcag ctgaagtggt
     3001 cgacgatgca aactgggcgg aaaactggaa gcagttctgg cacgccaata agatcggaaa
     3061 attcgtggtt gtgccgtcgt gggaaagcta tgaaaagcag ggagcagaaa ttgtgctcaa
     3121 cctggatccc ggcatggcct tcgggacggg agcccacgaa accacagctt tggttttaga
     3181 gttcttggga gaggttccag tggtcccgga aaccgtctac gatatcggtt gcggttccgg
     3241 gatactagct atcgcagcag ccaaactcgg tgctgaagtc tacgcttttg acaccgaccc
     3301 tctggcggta gatgccactt tgaaaaacgc tgagaaaaac catgtggaag gtttgatcaa
     3361 ggtctttggg ggtaatctgt tagaggacga aagcctgttt cccgacaaag ttccggacct
     3421 agttcttacc aacatcatcg ccgaggttat cgtagagttt gttcccctct taaaagagaa
     3481 gctgggaacc tttacgtgga tcgtgtcggg aataatcagc gaaaggctgc ctatggttct
     3541 cggtgctctt gaagagcacg gttttgccgt caccaagagg aaggacagag gtgagtgggt
     3601 gg
//
LOCUS       DAOSDA010000150         3602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16433_length_3602_cov_3.012123, whole genome shotgun sequence.
ACCESSION   DAOSDA010000150 DAOSDA010000000
VERSION     DAOSDA010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16433_length_3602_cov_3.012123"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PLW82_04425"
     CDS             complement(<1..824)
                     /locus_tag="PLW82_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HOJ46553.1"
                     /translation="MKKETLNITGMTCAACANAVERSVRKLEGVESASVNLAVETLTV
                     EYDETAVDIKDVKDAVIRAGYGVEEPKTSNVKEILLPIEGMTCAACSTAVERALKKLP
                     GVEEAVVNYATDSAKITYDLTKTRFSDIKEAVAKAGYKALDLEVNAPDVTDRRQNEIQ
                     ALWRRFMVAVIFTVPLFYISMGHMVGLTLPSFLDPGVSPLNFALAQLLLTVPVMIAGY
                     RFYTVGFSKLFRLEPNMDSLIAIGTGAAFLYGVYAVLQIIKGHGHFAMDLYFESVGV"
     gene            complement(854..1120)
                     /locus_tag="PLW82_04430"
     CDS             complement(854..1120)
                     /locus_tag="PLW82_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOJ46554.1"
                     /translation="MDSAEKTKVLNLLKTSRGQIEGIIKMVEDDRYCVDISKQILAVQ
                     AQLKKANLQILEQHIKHCVKEAVLSGSEEEKINEIIELLDRYVK"
     gene            complement(1302..1958)
                     /locus_tag="PLW82_04435"
     CDS             complement(1302..1958)
                     /locus_tag="PLW82_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOJ46555.1"
                     /translation="MIKLITKKKNKTGVITMKKHLRTTPEIKEFLIEADSKFSPIISA
                     VGDIEVELEPDLFVSLVSTIVGQQLSNKVLEIIWNRFDTLLQGEVTPEKILNLPDEKI
                     REVGISYRKIEHIKNLAQKVLNGELDLVALESFSDQDVIEKLVELKGIGVWTAEMLLI
                     FSLGRNDVFSVRDLGLRRGVARLYDLGDVSDDEVAKIAAKWSPYRTFASLYLWRYFSS
                     "
     gene            complement(2084..2437)
                     /locus_tag="PLW82_04440"
     CDS             complement(2084..2437)
                     /locus_tag="PLW82_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46556.1"
                     /translation="MLALFLVLNEVDYLDDILAKFVEVGVKGATILESQGMASAMLES
                     ANRGVAMFASLGHLLDNAHPYNKTIFTVLDNEEILEKTVAGVAEVLKDHNKPGAGFMF
                     TVPIGSVYSLSKLGK"
     gene            complement(2464..>3602)
                     /locus_tag="PLW82_04445"
     CDS             complement(2464..>3602)
                     /locus_tag="PLW82_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOJ46557.1"
                     /translation="PNVSGYLVFGLLVGPSFVKLISASDIEAFTVISELALGIIAFSI
                     GSEFVVKEMKKLGKKIFIITLAEVVGAVFVVFSVMYFIFKQPFAFSVVIASMSAATAP
                     AATLLVIQQYKAHGPLTRTLIPVVALDDIFGIMAFGIAMALARLSIGRTQGSFIAMVA
                     TPLIEIVGSLVLGLIIGLLLVYIAKKATNRDSLQGASLAAIGVACGLAKMIGFSPLLT
                     CIVVGAVVANLLSASKRVFGSVNDFASPVYVLFFTLAGASLDLKILASVGLLGIAYIF
                     ARAFGKMFGVWVGAKSVKAEETVQKYLGLGLLPQGGVSIGLSVLVRQQLPEYAVPITT
                     IIMASVLVYEVMGPVFAKVAIEKAGEIDGLSKSQAPQMSQEVLG"
BASE COUNT          946 a          934 c          759 g          963 t
ORIGIN      
        1 acccccacgc tctcaaagta gagatccatg gcaaagtgtc cgtgaccttt tatgatttgc
       61 aaaacggcgt agacgccgta gagaaatgca gccccggtac caatagcaat gagggagtcc
      121 atgttgggct cgagtctgaa caacttgctg aacccaactg tgtaaaacct gtaacctgcg
      181 atcattacag gaactgtcaa gagaagctgc gccagggcaa agttgagtgg agatacccca
      241 ggatcgagga agctaggaag tgtcaaacct accatgtggc ccatggagat gtagaacaaa
      301 ggaacggtga aaatcaccgc caccatgaac cgcctccaaa gagcctggat ctcgttctgc
      361 ctgcggtctg taacatcagg agcattcact tctaaatcca acgccttata gccggctttg
      421 gcaactgctt ctttaatatc cgaaaacctt gtcttggtaa gatcataggt gatttttgca
      481 ctgtctgtag cgtagtttac taccgcttct tcgactccgg gaagtttctt taatgctctt
      541 tccacggctg tagagcacgc agcgcacgtc atcccttcaa tggggagcaa aatctcctta
      601 acgtttgagg tcttcggttc ttctactccg tagcctgcgc gaatcaccgc atccttcaca
      661 tcttttatgt ctactgcagt ttcatcgtat tccacagtga gagtttcaac tgcaaggttg
      721 acactggcgc tttcaacacc ttctagttta cgaacgctgc gctcaacggc atttgcgcaa
      781 gcagcacagg tcataccggt tatattcaac gtctcttttt tcatcttgtc caccaccaat
      841 cgccgaactc cggctactta acataccgat cgagaagctc aatgatctcg ttgatctttt
      901 cctcttcact gccgctcaga accgcttctt taacgcagtg cttaatgtgc tgctccaaga
      961 tctgcaggtt cgctttcttg agctgtgctt gaactgcaag gatctgcttg gagatatcca
     1021 cgcagtatct gtcgtcttca accattttaa taataccctc gatttgaccc cgtgatgtct
     1081 tgaggagatt caacacttta gttttttctg cgctatccaa taagacctac ctcctacagt
     1141 acatctagtt ttccacaaac aaaaatgact ttatgtccct ccccatatgg ggtgggctaa
     1201 ttcaattata actttccccc agctccttga caagagctga gggaaagttt cttagttcga
     1261 tgatattacc cgagttctgt gttatggacg atattccggt ttcaagaaga aaagtacctc
     1321 cagagataga gggatgcaaa agtcctgtat ggactccact tggctgcgat ctttgcaacc
     1381 tcgtcgtcgg aaacatctcc aagatcatac aagcgggcca cacctctcct caaaccaaga
     1441 tctcttactg aaaacacgtc gttcctgcct aaggaaaaga tgagcagcat ctctgcggtc
     1501 cagaccccta ttccctttag ttccaccagc ttctcaatca cgtcctgatc ggaaaagctt
     1561 tctagcgcca ctagatccag ttccccgttt aaaacttttt gggcaaggtt cttgatatgc
     1621 tcgatcttgc gataggaaat acctacttct ctgatcttct cgtctggtag gtttaagatc
     1681 ttctccgggg tcacctctcc ttggaggagc gtgtcgaacc tgttccagat aatctcgagt
     1741 actttgtttg aaagctgctg accaacaatg gtgcttacca gagatacaaa gaggtcaggc
     1801 tcaagttcca cctcaatatc gcctacagcg gaaataatcg gcgagaactt agagtccgct
     1861 tctatcagaa attccttgat ctctggagtg gttctaaggt gttttttcat tgttatcacc
     1921 ccggttttgt tctttttctt agtaattagc ttaatcatcc tagattccta tgtggatttt
     1981 gccggtacta tcctgcattg ggtaaaagcc tctgcagaaa tgaggtttgt aaaaagcgga
     2041 tgcgcttttt cgcgcacccg ctatatgaag gagtgaagtg ttgttattta cctagtttag
     2101 ataagctgta aacactgcca atgggcactg tgaacataaa cccggcccct ggcttgttgt
     2161 ggtctttaag cacttctgca acgccagcta ctgttttctc caggatttct tcgttgtcta
     2221 aaaccgtaaa gatggtctta ttgtacggat gggcgttgtc taaaaggtgc cccagacttg
     2281 caaacattgc cacgcctctg tttgcactct ccagcatggc gcttgccata ccctggcttt
     2341 caagtatcgt tgcacctttt acaccgactt caacaaactt ggccaagata tcgtcgaggt
     2401 agtcgacttc atttaacaca agaaataagg ccagcataat aatcccctct tgttttcgta
     2461 tttttaccct aacacttcct gcgacatctg aggagcttgc gacttgctta ggccgtcgat
     2521 ctccccggct ttttcaatag cgaccttagc aaacacaggg cccataactt catacaccag
     2581 tacgcttgcc ataatgattg tagtaatagg aactgcatac tccggaagct gctgccggac
     2641 taaaaccgaa aggccgatcg aaactccgcc ttgagggagc agcccgagac ctaagtactt
     2701 ctgaacggtc tcttctgctt taaccgactt tgcccctacc catacgccga acattttccc
     2761 gaaagctcta gcaaaaatgt aggcgatgcc tagtagtcca acagaagcga ggatcttgag
     2821 gtctaggctt gcccctgcca gcgtaaagaa caagacataa accggagaag caaagtcatt
     2881 taccgaacca aagacccttt ttgatgcgga caagagattt gccactactg caccgaccac
     2941 aatacaggtg agaagcggcg agaaaccaat cattttagcc aagccacaag ctacaccgat
     3001 cgctgccagg gacgcccctt gcagactgtc tctattggtt gccttttttg cgatgtaaac
     3061 taatagcaac ccgattatca gtcccaagac tagggatccc acaatctcaa tcaatggggt
     3121 tgcaaccatg gctataaacg atccctgggt gcggccgata gacagtcttg ccaatgccat
     3181 cgctatgcca aatgccatga tgccgaaaat gtcgtccaaa gccacaacgg gtattaacgt
     3241 tctggtgagc ggaccgtgag ctttatattg ttgaatgact aatagcgttg ctgctggcgc
     3301 agtggcagca gacatagaag caattactac gctgaaagca aaaggctgct tgaaaatgaa
     3361 gtacatgaca gagaaaacga caaaaacagc ccccacaacc tctgccaaag tgatgatgaa
     3421 gatttttttg cccagctttt tcatttcttt aacaacgaac tcgctgccaa tgctgaaagc
     3481 gataattccc agggcaagtt cactgataac tgtaaacgct tcgatatcgc ttgcacttat
     3541 cagcttcaca aacgatggcc ccacaagcag accgaacacc aaataccccg acacgttagg
     3601 ta
//
LOCUS       DAOSDA010000151         3602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16434_length_3602_cov_2.914012, whole genome shotgun sequence.
ACCESSION   DAOSDA010000151 DAOSDA010000000
VERSION     DAOSDA010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16434_length_3602_cov_2.914012"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..1782
                     /locus_tag="PLW82_04450"
     CDS             292..1782
                     /locus_tag="PLW82_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase family protein"
                     /protein_id="HOJ46558.1"
                     /translation="MPDRRVLLWVDASANYDKLGSRAEIRRFVGKARQSGVTTLVLDV
                     KPVDGSVLYRSRIAPFYEKAGHEFRQIHDPLAMLIAEARGYGMEVHASFNVFCEGASG
                     RGLAYQEENDWATHMYDLALYLELDDRSIEIERYNQMPKDGQLAYITSDLMDVLKVKG
                     AAALVKDGVVVEVVTSGEVSLKETDVVYGFGSAAEILQGSVGAKIKAEKEIRPITKWA
                     NAPHAFVNPLLPKVQAYEIALMEEVASHYQVDGIVLDRVRYHNGQCDFSDNTRMLFEK
                     YLGREVANWPEDIIKLSFENGRKKVEDGPLAKEWYYFRAKGIKDFFITARDRIKSLRP
                     DIVFGDYAGSWYPEYYALGVNWASEEYVPTGYDWAKPGYEATGYAEVLDYLTTGNYYE
                     DVYLADLEGKIKAKVGMRTEAGQSLEYKPWYSVEGACDIVNEKVLGKTPVYGGLYLLQ
                     YQNKPEVFKEAMKVCLTKSDGLMLFDLVYVDMYDWWDEIYEVVSQF"
     gene            1790..2605
                     /locus_tag="PLW82_04455"
     CDS             1790..2605
                     /locus_tag="PLW82_04455"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOJ46559.1"
                     /translation="MVRELLVDYHMHLENGPKTLEYVEQFLKVAEKRGISEIGFSEHC
                     YRFKEARHLLDNPWARERNVDSVSEYLELLTRAKQKGWPVKIGIEVDYIPEVEDEIRT
                     FLEKYQFDYVFGSVHWIGDFGFDLKENIPVWKRSDPGEVWREYFAILLKAARSGLFDS
                     LPHPDLVKIYSFYPEEDLSDFITEVIGEIAKTGVCLEASTAGWRKPVGEMYPSREILQ
                     VIRGFDIPITLASDAHYPEDVGRDFDRMIPILKEAGFTEFIRFDRRQKTAERF"
     misc_binding    2735..2933
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3057..>3602
                     /locus_tag="PLW82_04460"
     CDS             3057..>3602
                     /locus_tag="PLW82_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ46560.1"
                     /translation="MLVKNRLLIPGPSQVPPEVNAAMTRPIIGHRSGDFSKFHEQVAQ
                     KLKKIFQTENEVVILTGSGSAAMEAAVSSVVSPGDAVLCIVTGKFGERFAELAKVYGA
                     DLTVMESEWGDTYDLGAVAKLMEEKAFKLVCATHNETSTGVTNDIKSLGELCTKHGAL
                     LLVDAVSSMGGMDIRTDEWKVD"
BASE COUNT         1000 a          762 c         1016 g          824 t
ORIGIN      
        1 tcctagatat ttttaaccac tatgggagtt tttgggactt ggtggtataa tgtatctaaa
       61 ttcgactgca gggggtcaaa ctctagataa gatataccac gtagagcggt gtttaaacgc
      121 tggagatttg aagaagcggt cgtaaagaca aatagacaaa gcggtgcttg gttagtatac
      181 gctgggttct gggaaaggca gatttaggca tagacaggca gtctttcttt aggatctgga
      241 taagccaggc tttagattaa ttgcgaaaac aagctaactg gaggttggac aatgccagat
      301 agaagagttt tattatgggt tgacgcgtct gccaactacg ataaattggg gagcagagca
      361 gagatcagga gatttgtggg taaagccagg cagtcgggag tgaccactct ggtgctggac
      421 gttaagcccg tagatggatc ggtgctctac cgcagccgga tcgctccgtt ttatgaaaaa
      481 gccggtcatg agttcaggca aatccacgat cccctggcca tgttgatcgc agaagccaga
      541 ggatacggca tggaagtcca tgcgtctttt aacgtttttt gtgaaggggc aagcggcaga
      601 gggttggcct atcaggaaga aaacgattgg gcaacgcaca tgtacgatct agcgctctat
      661 ctggagttag acgaccgctc gatagagatc gaaaggtaca accagatgcc gaaggacggg
      721 cagctggcct acatcacgtc ggatcttatg gacgtactga aggtaaaggg tgcagctgcg
      781 ctggtgaaag acggggtagt tgtggaagtt gtcacatcag gggaagtctc tttgaaagag
      841 accgatgtgg tgtacggctt cggcagcgct gcagagattt tgcagggcag cgttggagca
      901 aagatcaaag cggaaaaaga gattcgccca ataaccaagt gggccaacgc acctcatgct
      961 tttgtgaacc cgttgcttcc gaaggtacag gcctacgaga ttgccctcat ggaagaggtg
     1021 gcatcccact atcaagtgga cggaatcgtt ttggatcgcg tcagatacca caacggtcag
     1081 tgcgatttta gcgacaacac cagaatgctt tttgaaaagt atctggggag agaagtagca
     1141 aattggcctg aagatatcat aaagctcagt ttcgaaaacg gcaggaagaa ggtggaagac
     1201 ggtcctctcg caaaagagtg gtactacttc cgcgccaaag gcatcaaaga ctttttcatt
     1261 acggccagag accgcattaa gtcccttcgg cccgacatcg tcttcggcga ctatgccggc
     1321 tcgtggtacc cggagtacta cgctttaggc gtcaactggg ccagcgaaga gtacgtacct
     1381 accggttatg actgggcaaa gcctggatat gaagccacag gctacgcgga agttctggat
     1441 tacctcacaa ctggaaacta ctacgaagac gtttatctgg cagatttgga aggcaagatt
     1501 aaggctaaag ttggtatgcg gaccgaagca ggccagtctc tagagtacaa accttggtac
     1561 tcggtggaag gcgcttgcga tattgtcaac gaaaaggtgc taggcaaaac tcccgtctat
     1621 ggaggcttat accttcttca gtatcaaaat aagcccgaag tgttcaaaga agcaatgaag
     1681 gtctgcctta caaagagtga tggcctcatg ctgtttgatt tggtatacgt cgacatgtac
     1741 gattggtggg acgagatcta cgaggtggtc agccagtttt agttgaacgg tggtgagaga
     1801 gttgcttgtg gattatcaca tgcacctaga aaacggacca aaaacccttg agtatgtcga
     1861 gcagtttcta aaagttgcag aaaagcgggg gatctctgag atcggctttt ccgagcactg
     1921 ctacaggttc aaggaagcaa gacacctttt ggacaaccct tgggctagag agcgcaatgt
     1981 ggatagtgtc tccgagtatt tggagctcct tacccgcgcc aagcagaagg gctggcctgt
     2041 gaaaatcggc attgaggtgg actacattcc tgaggtcgag gacgaaatcc gcacttttct
     2101 cgaaaagtac cagttcgact acgttttcgg ctcggtacac tggatcggag acttcggctt
     2161 cgacctgaaa gaaaacatcc cagtctggaa gagaagcgat ccaggtgagg tgtggcggga
     2221 gtattttgcg attttgctca aagcggcaag aagcggcttg tttgacagct taccccatcc
     2281 ggacttggtc aaaatctaca gcttctatcc agaagaagac ctctcggact tcattacaga
     2341 ggtgatcgga gagatcgcca aaactggtgt ctgcctagaa gccagtaccg ctgggtggcg
     2401 aaagccggta ggagagatgt atccgtccag agagattctc caggtaatca ggggttttga
     2461 tattcccatc acgctagctt ctgatgccca ctacccagaa gatgtgggca gggattttga
     2521 ccgcatgata ccgatcttaa aagaagcagg ctttacagaa tttatccgct tcgacagacg
     2581 ccaaaaaact gcagagcggt tttagagcca atttctacca gaaataccaa gaattcgtta
     2641 gataattcac agagagggcg gaaactcgca atatttgagg aaatatcggt tttgccaatt
     2701 tgccccgatc tatgtagact caaacgtaaa caaggaaaat gcaatgacgc aggagcagta
     2761 cctatacctc tagagagcca gtggctggtg gaaactggca catagggaat ccgcctgcag
     2821 agctgatggc aaacccatcc ggtagcgcac cgttaaagcg aaatgagtgg gttggatcga
     2881 taattgatcc ggccaatcag ggtggcaacg cgggaatacc tctcgtccct gactatatgt
     2941 cagggacggg agttttttta tacgaaaaaa ccagataagt ttccgtcttg gaacgtagcg
     3001 cgcacgtaat gagacgagat agcagagctt tgagaagaga aaaggagagg gtagtaatgc
     3061 tggtaaaaaa ccgactgcta attcctggcc caagccaggt gcctccagag gtaaatgctg
     3121 caatgaccag accgatcatc ggtcacagga gcggcgactt ttccaagttt cacgaacagg
     3181 tggcccagaa gctgaagaag atctttcaaa cggaaaatga agtggtgatt ctcaccggtt
     3241 cgggaagcgc tgctatggaa gcggctgtca gtagtgttgt aagcccaggc gatgcagttt
     3301 tgtgcatcgt gacaggtaag tttggcgaaa ggtttgccga gctggcaaag gtctacggcg
     3361 cagatctaac agttatggag tccgaatggg gagacaccta tgaccttggc gctgtggcaa
     3421 aactcatgga ggaaaaagca tttaaactgg tgtgtgctac gcataacgag acttctactg
     3481 gagttaccaa cgacatcaag tcccttggcg agttgtgtac aaagcacggg gctctccttc
     3541 tggtagatgc cgtctccagc atgggtggca tggatatcag aaccgatgaa tggaaagtgg
     3601 at
//
LOCUS       DAOSDA010000152         3595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16476_length_3595_cov_3.312147, whole genome shotgun sequence.
ACCESSION   DAOSDA010000152 DAOSDA010000000
VERSION     DAOSDA010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16476_length_3595_cov_3.312147"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3061)
                     /locus_tag="PLW82_04465"
     CDS             complement(<1..3061)
                     /locus_tag="PLW82_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOJ46561.1"
                     /translation="MQVMTMVIKYDYEPTDEQKKAINCLDRDVAVVAGAGAGKTRVLI
                     YRILNIVLQGKADFSNIVAITFTEKAAAEMKVKLRKEARRQMELNPLLKSRLSKAPLT
                     TISTIHGFCANLIRRYALKIGYVPEFAILDQDDASRLLIKAISEVLEENENADDRIFI
                     RFKARSYSLKENLEAAYNKIREFGYTVSEALEISLKSLEWAEEQAKKNLEAFNEIMKY
                     AYSKHNIADKRTRFVKELLPKWPEVEPVFRRLESQEELTAEGKKAIVDYLASFKGITS
                     VSATFDEYKDVILKGAQTIPFLFTVADQREVVEKFASLLEQIDARYTKLKQEAASLDF
                     TDLQLKALEILKHPDVKEQLQKEYRYIMIDEYQDTNYLQQQIVDEIWQEGVNLFIVGD
                     PKQSIYRFRGAEVALFEDTSSRIRKKGGENVTLTFNFRSNKTLIDFYNSFFANYMPGD
                     PSKPYAIGYEAARNFHHNPDEKALNIIACNYDEKAEPEKLPPKLWQARVISRLIKEAV
                     ESGEAEYRDFAVLFSKRSSGLSELEEEFARFGIPFTSGLSQNFFQREEIRECLSLIEF
                     LNNPTDDLSLAACLKGPFFSFSDEEIYRIMALKKELQCSSLYAAILGCGGAGFYVQKE
                     RQRFGIQQTAGNNLQDLAEHLAELCTYYGYMSLSDILHEAIKKTQFNLILLAQTMGRE
                     RLANVEKLLEIISQEVQKGRSNNEIVEVLRIQQEKAVPDAQVVLEDQNRVAVMTIHGA
                     KGLEFKHVIVAGLTDFEGRSGNSAHFDFHKEMGLMVSLPSPLPELGVSVPCADPRYFS
                     YLEKEKDEESYEDQRLLYVAFTRARERLHLVFAGKIITKKGEYANGIVKMFDWNFLAQ
                     KVWFKDLELSFPEYEYANFDAKRKVLLPEVKIAEAHLRPRYRALSPSALVQFDMCPRL
                     FYLEQRLKVPSRELIASTDFTQLELDFSYEDELPPAEGTPDKATIGTIFHEIFASAEN
                     LEEALEFLRESGYDNLEENYITILENFFRSRFFQSKGESEVPLSWYLP"
     gene            complement(3036..>3595)
                     /locus_tag="PLW82_04470"
     CDS             complement(3036..>3595)
                     /locus_tag="PLW82_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOJ46562.1"
                     /translation="DLARENFIVKSLEMSFGSGRVKGLQVGPYTLRGEIDRLDLLRGD
                     VSKTGWVEGYFVTDYKRSGTSIDTAKATNRFQIKAYMLAIKNLGFSPVLGGKFHALTG
                     ETDYRCSLWADEILGRRSRLKLSIEEFEKELEGELQRAKNAAERLYGGVYTPNYESKS
                     CRYCSFMALCQKPSRAEDAGDDNGD"
BASE COUNT          808 a          923 c          845 g         1019 t
ORIGIN      
        1 cgggcaagta ccacgaaagt ggtacttctg attcgccctt actttggaaa aacctggacc
       61 taaagaagtt ctccaggatg gtgatgtagt tttcctcaag gttatcgtag ccgctttcac
      121 gcaaaaactc cagagcttct tctaagttct ctgccgaggc gaagatctcg tggaagatgg
      181 tgccaatggt tgctttgtcc ggagtccctt ctgccggagg gagctcgtct tcatatgaga
      241 agtccaattc gagctgagtg aaatccgtcg aagcaatgag ctcgcggctg ggaaccttca
      301 gcctctgctc aaggtaaaac aagcgtggac acatgtcaaa ctgaaccaga gcagacggtg
      361 aaagggcgcg gtatcggggc cttagatggg cttcggcgat cttcacttcc ggcagtaaca
      421 cctttcgctt ggcgtcaaag ttcgcatatt cgtattcggg aaagctcagc tctaggtctt
      481 taaaccacac tttctgggcc aaaaaattcc agtcaaacat tttaacaatg ccattggcat
      541 actcaccctt tttggtgatt attttgcctg caaagaccaa atgcagcctt tctctggctc
      601 tggtaaaagc aacatacaga agtctttggt cttcatagga ctcctcgtct ttttcttttt
      661 cgaggtagct gaagtacctg gggtcggcac aggggaccga tactccgagt tcgggaagcg
      721 gactgggtag cgaaaccatc aggcccattt ctttgtgaaa gtcgaaatgg gcagagtttc
      781 cagaccgccc ttcaaaatcc gtcaaacccg caacaattac gtgtttaaat tccaaacctt
      841 tagctccgtg aatggtcatc accgcaacgc ggttttggtc ttccagcaca acctgagcgt
      901 cgggaacggc tttttcctgc tgtattctca gtacctccac gatttcgttg ttggatctgc
      961 ctttttgtac ctcctgggag ataatctcca aaagcttttc cacattggcc aatctctccc
     1021 ttcccatagt ttgggccagg aggatcaggt tgaactgggt tttcttgatc gcttcgtgga
     1081 gaatatctga aaggctcatg tagccataat aagtgcagag ttcagcaaga tgctccgcta
     1141 aatcctggag attgttccca gctgtctgct gaatgccaaa cctctgccgc tctttctgca
     1201 cgtagaaacc ggctccgccg catcccagaa tcgctgcgta aagactgctg cattgcagtt
     1261 cttttttcag agccataatg cggtagatct cttcgtcgct aaatgagaag aacggtcctt
     1321 taaggcaggc tgcaaggctc agatcgtctg tggggttgtt taaaaactcg attaaggaca
     1381 gacattccct gatctcttct ctttggaaaa agttctggct gaggcctgaa gtaaacggta
     1441 tgccgaatct agcaaactcc tcctccagct cggaaagacc gctacttctt ttagaaaaca
     1501 gcaccgcaaa gtcacggtac tctgcctctc cactttccac agcctctttg atcagacggg
     1561 agataaccct tgcctgccac agttttggcg ggagcttttc cggttcggct ttttcatcat
     1621 aattacaagc gatgatattc agagcttttt catctggatt gtgatggaag ttccgtgcgg
     1681 cttcatagcc aatggcgtag ggtttgctcg gatctcccgg catgtagttt gcgaaaaagc
     1741 tgttgtaaaa atcgatcagg gttttgttgg agcggaagtt aaaagtcagc gtgacatttt
     1801 cgccgccttt tttccggatc cttgagctgg tatcctcaaa aagggcaact tctgcgccgc
     1861 gaaagcggta gatggactgt ttgggatccc caacaataaa caggttcaca ccctcctgcc
     1921 aaatctcatc tacgatctgc tgctggaggt agttggtgtc ctggtactcg tcgatcatga
     1981 tgtagcggta ctctttctgc agctgttctt tgacatcggg atgctttagg atctctaaag
     2041 ccttcagctg cagatcggtg aagtccaaac tggctgcctc ctgcttcagt ttggtgtagc
     2101 gagcgtcgat ttgctccaag agactggcaa acttctccac aacctccctc tgatccgcta
     2161 cggtaaacag gaagggaatg gtctgtgcac ccttgaggat aacgtcttta tactcatcaa
     2221 atgtggcaga cactgaagtg atgcccttga agctcgctaa gtagtcgacg atagcctttt
     2281 ttccctctgc ggtcagctct tcctgtgatt cgagcctgcg gaaaacaggt tccacctcgg
     2341 gccatttcgg cagcaactct ttaacaaagc gcgttctctt atcggcgatg ttgtgtttgc
     2401 tgtaggcata cttcatgatt tcattgaacg cttctaggtt ctttttcgcc tgttcttctg
     2461 cccattccaa gctctttaag gaaatctcca gggcttcact aacggtatag ccgaactcgc
     2521 gaattttgtt gtatgcagct tctaggttct ccttcaaaga gtacgacctg gctttgaagc
     2581 ggatgaaaat gcgatcgtcg gcattctcgt tttcttctaa aacctcggag atagctttga
     2641 tgaggagtct cgatgcatcg tcttggtcga gaatggcaaa ctcgggtaca taaccgatct
     2701 tgagggcata tcttctgatc aagttagcac aaaagccgtg gatggttgat attgtcgtga
     2761 gaggtgcttt agatagtctg ctttttagga gcgggtttaa ttccatctgc cttctggctt
     2821 cttttctcag cttaaccttc atctccgctg cggctttttc cgtaaacgtg atagcaacga
     2881 tgttgctgaa gtcagccttc ccttggagca cgatgtttaa aatgcggtag atgaggactc
     2941 tggtctttcc tgcaccggcc ccggcaacca ctgcaacgtc cctgtccagg cagtttatgg
     3001 cctttttttg ttcatctgtc ggctcgtagt catacttaat caccattgtc atcacctgca
     3061 tcttccgccc gcgacggttt ttggcacaga gccataaaag agcagtacct gcagcttttg
     3121 ctctcgtagt ttggggtgta cactccgccg tatagccttt ctgcagcatt tttggcgcgc
     3181 tgcagctccc cttcaagctc cttctcaaat tcctcgatgc tgagttttag cctgctccgt
     3241 ctcccgagaa tctcatctgc ccacaaactg cacctgtaat cggtttcgcc agtcagcgca
     3301 tggaacttac cgcctaaaac cggcgaaaac cccaggttct tgatggctag catgtaggct
     3361 ttgatctgga accgattggt agctttagcg gtgtcaatag aggttcccga gcgcttgtag
     3421 tcggttacaa aatagccctc tacccaaccg gttttggaga catctccgcg gagcaaatcc
     3481 agtctatcga tctcacccct taaggtgtac ggtcccacct gcaggccttt cactctaccg
     3541 ctgccaaagg acatctccag gctcttaacg atgaagttct cccttgcaag atcct
//
LOCUS       DAOSDA010000153         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16498_length_3591_cov_3.018100, whole genome shotgun sequence.
ACCESSION   DAOSDA010000153 DAOSDA010000000
VERSION     DAOSDA010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16498_length_3591_cov_3.018100"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1602
                     /locus_tag="PLW82_04475"
     CDS             97..1602
                     /locus_tag="PLW82_04475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ46563.1"
                     /translation="MSKLRSNTKGILLVAGILMLVGTLIGYVALAGSTKVPSRYMGEI
                     NGTKIERTRFERNFALVWNQQYQASYGQLTGYDIEPMKAQYLLNVMMHEALVSEAEKM
                     GITVTDEEVNANLRELEEQFGGAEQLKTQLNYLGMTMEELKDDLKKDLVLGKLQDELL
                     KDVTVTDDEVRRQLEEVEASHILVTDRELAETILKALKDGADFAQMAKLYSEDPGSKD
                     SGGSLGYFGRGVMVQEFEDAAFGLDVGEVSDIVTSDYGYHIITVTDRKTADDATFEEK
                     KEEAREQLLNIKRSEIINKFVSEVQEKSDIVIYDAQLKGYQALMEGRIDDAIKAYTEA
                     TGYATSDPYLLGSLAEAYRRKGDLDKAWELNKQAVEISVDPNVMINMALVGETKIRNI
                     INAQDTEEEMSVYQWAAGDHEFAEAKKQISAIYDEIVAALSKASENSQGDLMTHYQLA
                     QAYRYFGDTEREDQELSIVSQIIAQYDDAEADDAALEDSEAVLEDAEIATE"
     gene            1706..2941
                     /gene="hisS"
                     /locus_tag="PLW82_04480"
     CDS             1706..2941
                     /gene="hisS"
                     /locus_tag="PLW82_04480"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOJ46564.1"
                     /translation="MISRPKGTNDLLPDNIWRWQKLEQLIREECARFNYHEIRTPIFE
                     ETGLFQRGLGEATDIVEKEMYTFDDKGGRSMTLRPEGTASVVRAYIENNLQGTGLPVK
                     LYYLGPMFRYERPQAGRYRQFHQFGVECFGSSEPSLDAEVIVLAYSIYTKLGAKNVQL
                     KLNSIGCPNCRPKYLEKLKEALKERRDSLCDNCRKRSETNPLRVLDCKEKDCQPAIAE
                     LPRITDYLCDECRDHYGKVKSILKLWNVEYVEDSRLVRGLDYYTKTVFEFLVESLGAQ
                     NAVGGGGRYDGLVEEIGGEPTPAVGFAMGIERTILAVGDSWQKDPDVDLYIVAIGDTK
                     DAALTQLKLARDHGLKAEMDHLNRSVKAQFKQANRLNAKYCLILGPDELANNLVKLRN
                     MQDGEEIQLSLNDWLGGIK"
     gene            2942..>3591
                     /locus_tag="PLW82_04485"
     CDS             2942..>3591
                     /locus_tag="PLW82_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOJ46565.1"
                     /translation="MLKDAHCGLLRKTDAGKVVTLCGWVHRRRDHGGLIFVDLRDRSG
                     LVQVVFDPETSRENFALAEKLRSEFCVRITGEVSIRPEGLENKEIATGDIEVKAQKLE
                     ILSQSQVLPFAVDTRVDVDENVRLKYRYLDLRREELQKNLILRHNITKAVRDFLDNDG
                     FLEIETPMLTKSTPEGARDYLVPSRVNPGSFYALPQSPQIFKQLLMVSGFEKYFQIA"
BASE COUNT         1050 a          793 c          943 g          805 t
ORIGIN      
        1 ctgctcggtc tagttaattg acaaccgtgc tgaaacttag gtaaaattat atagtatttg
       61 ctgggctatt taaggagggg aaaggttttg actttaatgt ctaaactgcg ctcaaatacc
      121 aaaggcatct tgcttgtcgc aggtattttg atgctcgttg gaacactgat aggttacgtt
      181 gcactagctg gctccacaaa agtaccttcg aggtatatgg gagagattaa cggaacgaaa
      241 atcgaaagaa cccggtttga gcggaacttt gctctggttt ggaatcaaca gtaccaggca
      301 agttacggtc agctcactgg atacgatata gagcccatga aggcacagta tctgttgaac
      361 gttatgatgc acgaagcttt agtaagcgaa gcagaaaaaa tgggaataac agttactgac
      421 gaagaagtaa acgccaactt gagagaactt gaagagcagt tcggcggtgc cgaacagttg
      481 aaaactcagc tgaactacct gggcatgacc atggaagagc tgaaagacga tctcaagaaa
      541 gatctcgttc tcggcaaact ccaagatgaa ctactcaagg acgttaccgt aaccgatgac
      601 gaagttcgca ggcagcttga ggaagtggaa gcaagccaca tcctggttac agatagagaa
      661 ctagcagaga ccattctcaa agctctcaaa gacggtgcag actttgccca gatggctaag
      721 ctgtactctg aagacccagg ttccaaagat tccggaggtt ctttgggtta ctttggccgt
      781 ggtgtaatgg tccaagagtt cgaagatgca gcttttggtc ttgatgtagg tgaggtttcc
      841 gatatcgtta catcagatta cggttatcac atcattacag ttacagaccg caagactgca
      901 gacgatgcaa ctttcgaaga gaagaaggaa gaagccagag aacagcttct gaacatcaag
      961 agaagcgaga ttataaataa gttcgtctca gaggtccagg agaagtccga catcgtgatt
     1021 tacgatgccc aactcaaagg ttatcaagct ttgatggaag gcagaatcga tgatgccatc
     1081 aaggcataca ccgaagctac cggatacgct acctccgatc cgtaccttct aggctccttg
     1141 gccgaggcat atcgcagaaa aggcgatttg gacaaagctt gggaacttaa caaacaggca
     1201 gtggagatct ccgtcgatcc taacgtcatg atcaacatgg ccttggtcgg agagaccaag
     1261 atccgcaata tcatcaacgc tcaggacacc gaagaagaaa tgagtgtcta ccagtgggca
     1321 gctggagatc acgagtttgc agaagccaag aagcagatca gcgctatcta tgatgaaata
     1381 gtcgctgcac tgagcaaagc ttcagagaac tctcaaggcg acctcatgac tcactatcag
     1441 ctggctcaag cctacaggta cttcggcgat acagaaagag aagaccagga gctaagcatc
     1501 gtttcccaga tcatagctca atacgatgac gcagaagcag acgatgcagc ccttgaggat
     1561 tcagaagcag tcctggagga cgcagaaatc gctactgaat agtcctcttt gacaagcaca
     1621 gctatttgtg cagagatttt ttggggaagt ggtggcactt gtcaccactt ctctttataa
     1681 ttagggatat gaagaggtga taaacgtgat atcacgccca aaaggaacca acgatctgct
     1741 tccggataac atatggcggt ggcaaaagct agaacagctg atccgcgaag aatgtgccag
     1801 gttcaactac catgaaatac gcacgcctat cttcgaagaa acagggcttt ttcaaagagg
     1861 cttaggcgaa gcgaccgata tcgttgaaaa agagatgtat acctttgacg acaagggcgg
     1921 aagaagtatg accctgcgtc cagagggaac agcatctgtt gtcagggctt acatcgaaaa
     1981 caatttacag ggcactggtc tcccggtgaa actctattac ttaggtccta tgttcaggta
     2041 cgaaaggcct caggccggca gatacaggca gtttcaccag ttcggtgtgg aatgcttcgg
     2101 gtcgtcggaa cccagcctag acgctgaagt aatcgtcctt gcctacagca tttacactaa
     2161 gctcggagca aagaacgtgc agctgaagct caactccatt ggctgcccta actgcaggcc
     2221 caagtatcta gagaagctga aagaagcatt gaaagagcgt agagactcgc tgtgcgacaa
     2281 ctgcagaaag cgttctgaga ccaatcctct cagggttctc gactgcaagg agaaggactg
     2341 ccagccagca attgcagaac ttccccgcat caccgactat ctctgcgacg aatgcagaga
     2401 tcactatggg aaggtcaaaa gcattctcaa gctgtggaat gtagagtacg tagaggactc
     2461 caggcttgtt cgtggccttg actactacac caaaacggtg ttcgagtttc tcgtggaaag
     2521 tctcggtgcc cagaacgctg ttggcggtgg cgggcgctac gatggacttg tcgaagagat
     2581 cggcggtgag cctaccccgg ctgttggctt tgccatggga atcgaaagga caatcctcgc
     2641 tgtgggagat tcatggcaaa aggatccaga cgtggatcta tatattgtgg caatcggtga
     2701 tactaaagat gccgcattga ctcagctgaa gctggcaaga gatcacggcc taaaggcaga
     2761 gatggatcac ttaaacagat cggttaaggc tcagttcaaa caggcaaata ggcttaatgc
     2821 caaatactgc ttgattttgg gacctgatga actggcaaac aacttggtta aactgagaaa
     2881 catgcaagat ggtgaagaga tccagttatc actcaacgac tggttagggg gcataaagta
     2941 gatgttgaaa gatgcacact gcggtttact gcgaaaaacc gatgcaggca aagtggtaac
     3001 tctgtgcggc tgggtgcacc gccgcagaga ccacggcggc ttgatctttg tcgatcttag
     3061 agatcgctca ggcttggttc aggtagtttt cgatccggag acaagcagag aaaactttgc
     3121 actggcagaa aagctcagat cggagttttg cgtaaggatt acaggtgagg tctccatcag
     3181 accggagggg ttggagaaca aagaaatcgc cacaggcgat atcgaggtaa aagcccagaa
     3241 gttggagatc ctttcccagt cccaagtatt gccgtttgcg gttgatacta gggtagatgt
     3301 ggacgaaaat gtgcgcctca agtaccgcta tttggacttg cgtcgcgagg aactgcaaaa
     3361 aaacttgatt ttgaggcaca atattactaa agcagtaagg gacttcttgg acaacgatgg
     3421 tttcttggag atcgagacgc caatgctgac caagtccaca ccggaaggtg ccagagacta
     3481 tctggttccc agccgtgtca atcccggcag cttctatgcg cttccccaat caccccagat
     3541 tttcaaacag ctgttgatgg tctcgggctt cgagaagtac ttccagatcg c
//
LOCUS       DAOSDA010000154         3586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16532_length_3586_cov_2.843104, whole genome shotgun sequence.
ACCESSION   DAOSDA010000154 DAOSDA010000000
VERSION     DAOSDA010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16532_length_3586_cov_2.843104"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1081)
                     /gene="pfkA"
                     /locus_tag="PLW82_04490"
     CDS             complement(86..1081)
                     /gene="pfkA"
                     /locus_tag="PLW82_04490"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOJ46566.1"
                     /translation="MKVIGILTSGGDAPGMNAAVRAITRVANKLGMDVFGIYHGFAGL
                     IEDEIIRLDSRKVGDVLHRGGTFLKTARSPEFMTEEGQAKAVANIKKYGMQGLAVIGG
                     DGSFKGALALQRHGVKVVGIPGTIDNDIPCTDYTIGFDTAINTVVDCVNKIRDTATSH
                     ERTFVVEVMGKNCGDLALHAGLAGGAEVILIPEVEPDYDAVCKKVQKSHHQAKSHAVV
                     IVAEGVGGDPAYRKDYLSAAFHVAELINKRTGIETRVTILGHIQRGGSPTASDRLLAS
                     RLGAEAARALYEEDTGKMIAIQDGKVVRVDLDYALSQKKTVKREYLELANMLSSV"
     gene            complement(1176..2138)
                     /locus_tag="PLW82_04495"
     CDS             complement(1176..2138)
                     /locus_tag="PLW82_04495"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003355621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOJ46567.1"
                     /translation="MGNTLILEFEKPIVELEKRIEELKIFARERDLDLSDEINNLQVR
                     CDKLKRDIYSSLNSWQRIQLVRHPQRPTALELIDLIFDDFFEMHGDRLFGEDSAMIGG
                     IALFKGEPVTVIAPQKGRDTKENIERNFGLPHPEGYRKAIRLMKQAEKFGRPVITLVD
                     VVGAYPGIAAEERGQGPAIAEAITVMGSLQVPTIAIITGEGGSGGALALAMGNRVLML
                     ENAYYSVISPESASIILYKNDQHAKEAAEALKITSRDLLEMHVIDEIIPEPLGGAHKD
                     PKQMANLVKEALDRNLSILKALSKEELVASRYERFRVLGAIAAQ"
     gene            complement(2144..3007)
                     /gene="accD"
                     /locus_tag="PLW82_04500"
     CDS             complement(2144..3007)
                     /gene="accD"
                     /locus_tag="PLW82_04500"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOJ46568.1"
                     /translation="MFKDLFRSKPKYVTVRPNRQQDFSEKKEIPDGLWVKCDKCSNIL
                     YKKDLERSFSVCPKCGFHFVLSAAKRIEQIADENSFEEMDADLETADPLGFPSYMEKL
                     KKYQEESKMKDAVITGRAKIGGIKTVLIVIDFNFMRGSMNTVVGEKIVRAFETAVKEE
                     LPVVVISGGGGGARMQEGVLSLMQMARTCLAVEKHSQAGLLYIAVLTQATMGGVYASF
                     ASQADIILAEPGALIGFAGPRIIEQTMKQSLPEGFQTAEYVHDHGHIDHIVARPFLKD
                     TLAHLLAWHVR"
     gene            complement(3070..3327)
                     /gene="mtrB"
                     /locus_tag="PLW82_04505"
     CDS             complement(3070..3327)
                     /gene="mtrB"
                     /locus_tag="PLW82_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp RNA-binding attenuation protein MtrB"
                     /protein_id="HOJ46569.1"
                     /translation="MNQNSGLVGRSDFIIVKALEDGVTVIGLTRGENTKFHHTEKLDA
                     GEVMIAQFTSNTSAMKIRGKAEIITEHGSVTSGGLKNKTNS"
     gene            complement(3353..>3586)
                     /locus_tag="PLW82_04510"
     CDS             complement(3353..>3586)
                     /locus_tag="PLW82_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOJ46570.1"
                     /translation="LAARAKAISEGMKVAAADAISECVTVLDRNNIIPSPFDPQVPAK
                     VARRVAEAAMAEGLAKVSVDLDQLERAILERNR"
BASE COUNT          824 a          968 c          785 g         1009 t
ORIGIN      
        1 gaagaggggc cgattgtaca aacaatctta gtcttgcgca agagaacacc tccaattgtc
       61 actccaaaat atcaatgggc tactgttaaa cgctagaaag catgttagcg agctccagat
      121 actcgcgttt tacagttttt ttctgggata gtgcgtagtc taggtcaact cgcacaacct
      181 tgccatcctg gatggcaatc atcttacctg tatcttcttc gtaaagggct ctggctgctt
      241 ctgcaccgag cctgctggca aggagcctat cgcttgctgt gggagagcct cctctttgaa
      301 tatgaccgag gatagttacc cgcgtttcaa taccagttct cttgttgatc agctcggcga
      361 catgaaacgc cgcagacaga tagtccttgc ggtatgcggg gtcaccgcct actccttcgg
      421 ccacaatcac tactgcatga gacttcgctt ggtggtggct tttttggacc tttttgcaca
      481 ctgcatcgta gtccggttcc acttccggaa tcagtattac ttcagctccc cctgcaagtc
      541 ctgcatgtag agccaggtct ccgcagtttt tgcccatgac ttccacaacg aacgtccgct
      601 catggctggt tgcagtgtcg cgaatcttgt taacacaatc tacaacggtg ttgattgccg
      661 tgtcaaaacc gatggtgtag tcggtgcagg ggatgtcgtt atcgatggtt cccggaatac
      721 ccacgacttt aacaccgtgc ctctggaggg caagggctcc tttaaacgaa ccgtctccac
      781 cgattactgc cagtccctgc attccgtatt tttttatgtt ggctacggct ttcgcctgac
      841 cctcttcagt catgaactct ggagagcggg cggttttaag aaaggtaccg ccgcgatgca
      901 gaacgtcacc aaccttgcgg gagtcaagac gaatgatctc gtcttcgatg agaccggcaa
      961 agccgtgata aatgccaaac acatccatac ccagtttatt tgcaactcta gtaatagctc
     1021 tgactgcagc gttcatgccg ggggcatcgc cgccactagt tagaatacca ataaccttca
     1081 cttcattatt acaaaaaagg catgctcacc ttctttgtat agtcacctct cttctacaga
     1141 aacatcaaga acagatcctt cgtaagctaa agccgttatt gtgcagcgat tgctccaagg
     1201 accctgaagc gctcgtatct agaagctacc agttcctctt tgcttaatgc tttcaagatt
     1261 gacaggttgc gatcgagagc ttctttgacc aggtttgcca tctgtttggg gtctttgtgg
     1321 gctcccccta aaggctctgg aataatctcg tcgatcacgt gcatctccaa gaggtcgcgg
     1381 ctggtaatct tcagtgcctc ggcagcctct ttagcgtgct gatcgttttt gtaaagaata
     1441 atcgaggcac tctccggcga aatcaccgag tagtaggcat tttccagcat taagaccctg
     1501 ttacccatgg ccagagctaa cgctccgccg ctaccgcctt cgccggtgat gatggcgatg
     1561 gtcggaactt gcagagaacc catcacggtg atggcttctg cgatcgccgg tccttggcct
     1621 ctttcttcag ccgcaatccc ggggtaggcc cctaccacat ccaccagagt tattactggt
     1681 cgcccgaatt tttccgcctg tttcatcaag cggatagctt tgcgatagcc ttcaggatgg
     1741 ggaagtccaa aattcctctc gatgttctct ttagtatctc tgcccttctg cggcgcaatg
     1801 accgttacag gctcgccttt aaacaaggca atacctccga tcatggcaga gtcttcgcca
     1861 aacagcctgt ctccgtgcat ctcaaaaaag tcatcaaaaa tcagatctat gagttctaga
     1921 gcggttggtc tttggggatg tcgaaccagc tgtatgcgct gccaggagtt cagactcgag
     1981 taaatatcgc gttttagttt atcgcaacgg acctgcagat tgttgatctc atcagatagg
     2041 tccagatcgc gctctcttgc aaagatcttc agttcttcga tgcgcttttc cagttctacg
     2101 atgggttttt caaactcaag tatcaaggta ttgcccaaaa ccctcacctc acatgccaag
     2161 ctagaaggtg tgctaacgta tccttcaaaa atggcctggc tacaatgtga tctatgtggc
     2221 cgtgatcgtg tacgtattct gcagtctgga agccttcggg caggctctgc ttcatggtct
     2281 gttcgataat cctcgggcct gcaaatccga tcagagctcc gggctctgcg aggataatat
     2341 cagcttgaga agcaaaactg gcatagacgc ctcccatggt agcttgggtc agaactgcaa
     2401 tgtacaggag gcctgcttgc gaatgctttt caactgccaa acaggttcgt gccatctgca
     2461 tgagcgaaag aacgccctcc tgcattctgg cgcctccacc accgccgctg atcacgacca
     2521 caggaagctc ttcctttaca gctgtctcaa aagctctaac gattttctcg ccaacaactg
     2581 tgttcatgga gcctctcata aagttgaaat caataacaat cagtacagtc ttaatgccgc
     2641 ctatcttagc tcggcctgtg attacagcat ctttcatttt gctctcttct tggtacttct
     2701 tcagcttttc catataactg gggaaaccaa gaggatctgc agtttccaga tctgcatcca
     2761 tctcttcgaa actgttttca tctgctatct gctcaatgcg tttggctgca gacagaacaa
     2821 aatggaaacc gcactttggg cagaccgaaa aactcctctc caagtccttc ttatagagga
     2881 tattactgca tttatcacat ttaacccaca gcccgtcggg tatctccttt ttctcggaaa
     2941 aatcctgctg cctattgggt ctaactgtta catatttagg cttcgatcta aacaaatcct
     3001 taaacacaga ccttatcacc ccttacggtc tgacgcatgc tgctttttct ttgaaattat
     3061 aacatatcct caagaatttg tcttattctt caaaccccct gatgttaccg atccatgctc
     3121 ggtaatgatc tctgcttttc ccctaatttt catagctgac gtgttgctgg tgaactgcgc
     3181 gatcatgact tcaccggcgt ccagcttttc tgtatggtga aatttggtat tttcgcccct
     3241 tgtcagtcca ataacagtta ctccgtcttc aagagcctta acgatgatga aatcgcttcg
     3301 gccgacaagc cctgagtttt ggttcactta aagcacctcc cctgtaaact agctatctat
     3361 ttctttccag aattgctctc tccaactggt cgaggtcaac gctgaccttg gccaaacctt
     3421 cagccatagc cgcttctgcc acccttcgtg caacctttgc gggcacttgt ggatcgaagg
     3481 gcgaaggaat gatgttgttt ctgtccagga cagttacaca ctcgctgata gcatctgctg
     3541 ctgcgacttt cataccctcg ctgatggctt tagctctagc tgcaag
//
LOCUS       DAOSDA010000155         3583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16556_length_3583_cov_2.960317, whole genome shotgun sequence.
ACCESSION   DAOSDA010000155 DAOSDA010000000
VERSION     DAOSDA010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16556_length_3583_cov_2.960317"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLW82_04515"
     CDS             <1..667
                     /locus_tag="PLW82_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOJ46571.1"
                     /translation="TIAFGRGGDVEAEDVVFDEENRATFTLKVLGQSERIALALPGSH
                     NVENALAAAGAAAYAGIELGKIKRALENLTQVSGRLEEVRVLNLIVIDDTYNASPLSM
                     KAALEHLRRRSDALGLKAYAALGDMKELGSEAAEYHRTIGALCAELNLDGLYLYGSLM
                     EQYALSEARRLGLSAEAFSTHAEIAENLKDLTGIVLLKGSRSMEMERVLAYLKEFAAE
                     KNS"
     gene            698..1660
                     /gene="mraY"
                     /locus_tag="PLW82_04520"
     CDS             698..1660
                     /gene="mraY"
                     /locus_tag="PLW82_04520"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOJ46572.1"
                     /translation="MTNPIFAGILSLIIVLLIGPKLIGSLHKLKYGQTVRSDGPKTHL
                     KKSGVPTMGGLLFITGTTIATLIFARSSDEALISLAVFLGYGLIGFLDDYIIVKKKRT
                     LGLKARYKLFFQIVLGLLIGLWAAYKPGIGTLVYWPFGGAWELPVWLFVPFAAFVLVA
                     VTNAVNLTDGLDGLASGTVVIALLPFVLMCYRSGRIGLLTFAVALVGSLLGFLYFNRH
                     PAKIFMGDTGSLALGAALGAISVLTGTEFFLAIIGGLFVIEVLSDIIQVTVFKKTGKR
                     VFKMAPIHHHFELLGWHETKVVGTFYLAAAVFAAIGTVLIIRIW"
     gene            1677..2762
                     /gene="ftsW"
                     /locus_tag="PLW82_04525"
     CDS             1677..2762
                     /gene="ftsW"
                     /locus_tag="PLW82_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOJ46573.1"
                     /translation="MQRKGQIDLWLLLTASLLLIIGLISLLSASSVISYQDYGNPYAI
                     FFRQCLFAVVGLVAAVVALFFPLDKLRKMSWLIYAVAVALLVLVLFVGEVTKGSRRWI
                     DLGIGTLQPSEVAKLALVIFLADYLSKVKEKIRTVKGIGIALITMAIVGILIVLEPDL
                     GTTVAAMATCYFMLMAAGAKSSHLWLLVVLGILGVIGLAVIEPYRMKRLMTFLNYEND
                     PLGDGYQLIQSIFAFGSGGIVGVGLGQSKQKYLYLPENHTDFIFPIVGEELGFIGTFF
                     VVLLFGIYAWRGLRIAIKSKDSFHSFLAVGLTMQIAFQAFLNMGVVTGVLPVTGLTLP
                     FFSAGGSSLLITLTSVGLLLNVSRYVE"
     gene            2796..>3583
                     /locus_tag="PLW82_04530"
     CDS             2796..>3583
                     /locus_tag="PLW82_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOJ46574.1"
                     /translation="MRVVVTGGGTGGHIFPALAIANALDAEILYIGGEKGIEKDILPD
                     SGFKYKLIDVEGFKRKLSIKNVAIALKALTATKRCRAILREFKPDVVVATGGYVSGPV
                     GQAAAGLKIPLFLQEQNSYPGVTVRLLAKKARAIFLGSKGAEKYLPKHKCMFTGNPVR
                     REIVDADRKEARRRLQLEKKTLLLVTGGSQGSQAINEAVRALYPKLKKKKDLVVVHHT
                     GWLDYPDVKSGPTAYPVLQSELGQGAKAVAENIFITPFIKGMAT"
BASE COUNT          843 a          847 c          978 g          915 t
ORIGIN      
        1 cacaattgct tttggcagag gtggagatgt ggaagcagag gatgtggttt tcgacgagga
       61 aaaccgggcg actttcactt tgaaagtttt ggggcagtcg gagagaattg ccctggcact
      121 tcccggatct cacaacgtgg aaaacgctct ggctgcagct ggtgctgcag cctatgcagg
      181 tatagagctt ggaaagatca aaagggcttt ggaaaacctc actcaagtct cgggaaggtt
      241 ggaggaggtt cgagttctta acttgattgt gatcgacgac acctacaatg ccagtcctct
      301 ttccatgaag gcggcacttg agcatctcag gcggcgatcg gacgcattgg gcctgaaggc
      361 ttatgcagcc ctcggcgaca tgaaggaact cggaagcgaa gcagcggagt accaccgcac
      421 cattggcgcc ctctgcgcag agttgaactt agatggtttg tatctttacg gctccctcat
      481 ggagcagtat gctctaagcg aagcaagaag gcttggactg agcgcagaag cattttccac
      541 ccacgctgag atcgcggaga atttgaaaga tctgacagga atcgtgctcc ttaaaggctc
      601 ccgttccatg gaaatggaaa gggtactggc ttaccttaag gagtttgcag ccgagaagaa
      661 cagttgaagg aggcagcctc ccaagtagga gcattctatg actaatccaa tatttgcggg
      721 aatactttct cttatcatcg ttttgcttat cgggcctaaa ctcatcggca gcctgcataa
      781 acttaagtac gggcagaccg tgcgcagcga tgggccaaag acccacctaa aaaaaagcgg
      841 cgtcccgact atgggcggac tattgttcat taccggaacg actatcgcaa cgcttatctt
      901 cgctcgctca tctgacgaag ccctgatcag cctggctgtg tttcttggct acggcttgat
      961 tggctttttg gatgactaca tcatcgttaa gaagaagcgc acattagggc taaaagcacg
     1021 ctacaagctg tttttccaaa tcgtgctcgg gcttcttatc ggcctttggg cagcatacaa
     1081 accgggcata ggcacccttg tctactggcc tttcggagga gcatgggagc ttccagtctg
     1141 gctgtttgtt cccttcgctg cctttgtttt ggtagcggtg accaacgccg tgaacctcac
     1201 cgatgggttg gatggtctgg cctctgggac agttgtcatt gcgcttttac cgtttgtcct
     1261 catgtgctac agatccggca ggatcggtct tttgaccttt gcggtggcgc ttgtaggaag
     1321 cctcttggga tttctctact tcaacagaca cccggcgaag attttcatgg gcgataccgg
     1381 atcgctggct cttggtgcgg ctttgggagc gatcagcgta ctgacaggca cagagttttt
     1441 cctggcaata attggggggc tttttgtaat cgaagtgctt tcagatatca ttcaagttac
     1501 ggttttcaaa aaaaccggaa agcgggtctt caaaatggcc ccgatccacc accacttcga
     1561 acttctaggg tggcatgaaa ccaaggttgt gggaaccttt tacttggcgg cagcggtgtt
     1621 cgccgctatt ggtacagtcc ttatcattag gatttggtaa tagagaggtg aggtgaatgc
     1681 agcgcaaggg acaaatcgac ctctggctgc tgcttaccgc aagtttgctt ctgatcatag
     1741 gtctgatttc gctgctcagt gcaagctcgg taatctccta tcaagactac ggcaacccct
     1801 acgcgatttt tttcagacag tgtctgtttg cagtcgtagg gttggttgca gcagttgtcg
     1861 ccctgttttt ccccctggat aaactgagga aaatgagctg gttgatctac gctgttgcgg
     1921 tagccctgtt ggtcttagtt ttgtttgtcg gcgaagtaac caagggctct cgaagatgga
     1981 tcgatctagg catcggtacc ctccaaccgt cggaagtcgc aaagctggct ctggttattt
     2041 ttcttgcaga ttatttgagc aaggttaagg agaagatcag gaccgtcaag ggcatcggca
     2101 ttgccctcat tacaatggcc attgtcggga ttttgatcgt gttggaacca gatctgggca
     2161 ctacggtagc agccatggct acatgctact tcatgctgat ggctgcaggt gccaaaagca
     2221 gtcacttgtg gctcctggta gtgctgggga tccttggggt aattgggctt gcagtaatcg
     2281 aaccgtaccg catgaagcgc cttatgacct ttctcaacta tgaaaacgat ccgctaggag
     2341 acggctatca gcttattcaa tcgatctttg ctttcggctc cggcggcatt gtaggagtgg
     2401 gactgggtca gtccaaacag aagtaccttt accttcccga aaaccacacg gacttcattt
     2461 ttcctattgt cggtgaagaa ctcgggttta ttggcacgtt tttcgtggtt ctcctttttg
     2521 gcatctatgc ctggagaggg ctgagaattg ccatcaaatc aaaagactcg ttccacagct
     2581 ttcttgcggt gggtctgacc atgcagattg cctttcaggc ttttttaaac atgggtgttg
     2641 ttacaggtgt cctgcctgta acgggtctga ctctgccgtt tttcagtgca ggcggatcgt
     2701 ctcttctcat taccctaacc agtgttggtc tgttgttgaa cgtttcacgg tatgtggagt
     2761 aggcagaaca aagacgttca gatggagatg atcaaatgag agtagttgta accggaggtg
     2821 gaacaggtgg ccacatcttt ccggctttgg ccattgccaa cgctttagac gccgagattc
     2881 tgtacatcgg tggggaaaaa ggcatcgaaa aagacatcct gcctgactcc ggatttaagt
     2941 acaaactgat agatgtcgag ggattcaaaa ggaagctgtc gatcaaaaac gttgcaatcg
     3001 cgctgaaggc cttgacggca acgaaaaggt gcagggcgat tttgcgagag tttaagcccg
     3061 acgttgtggt agcaaccgga gggtatgtct ccggcccagt ggggcaggcg gcagctggtt
     3121 tgaagatccc tctctttctc caggaacaga actcctatcc cggtgtgacc gtcaggctcc
     3181 tggccaaaaa agcacgagcg atttttctgg ggagcaaagg tgccgaaaag tacctgccaa
     3241 aacacaagtg catgtttacc ggcaacccag tacgcagaga aatagtggac gccgatcgaa
     3301 aagaagccag acgcaggctt caactagaaa aaaagacgct gcttcttgta acaggcggct
     3361 ctcagggttc tcaggccatc aacgaggcag tgcgcgcttt gtaccccaag ctcaaaaaga
     3421 agaaggacct ggtggtagtt caccacaccg gttggctgga ctaccctgat gtaaaaagcg
     3481 ggcctaccgc ttatccggtc ctccaaagtg aactgggcca gggcgcaaaa gccgtggcag
     3541 aaaacatctt catcactccc tttatcaaag ggatggccac gtt
//
LOCUS       DAOSDA010000156         3576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16604_length_3576_cov_2.991196, whole genome shotgun sequence.
ACCESSION   DAOSDA010000156 DAOSDA010000000
VERSION     DAOSDA010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16604_length_3576_cov_2.991196"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PLW82_04535"
     CDS             <1..508
                     /locus_tag="PLW82_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOJ46575.1"
                     /translation="DEKKIELAKKAGVDLAGHAKEIGADAIKSFLGQGVDAVVMTAGS
                     SSESLVNETLKALRPGGRYIMVGVSPLNINREIMFQLESEVMVTRAGGPGRYDRQYEL
                     HGTDYPLQYVRWTEGRNMQEFIRLLEKGRISTKELITHRYHFEDAAKAYEMIQSGKEE
                     YIGVILTY"
     gene            543..980
                     /locus_tag="PLW82_04540"
     CDS             543..980
                     /locus_tag="PLW82_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46576.1"
                     /translation="MEKGARKIVRTPKTPGKISVGDKAEVIMGTDVYNYLMESTDGVP
                     VGKDLHPMTTFPCGLAIDAATEATSYDLTGVGTLKAMSFDLENPIQPEGELVGSAEVV
                     LIGRRHVEVAVEVVQNQRVILNGRVIMVKVRDGRAQELGDLVG"
     gene            1064..1282
                     /locus_tag="PLW82_04545"
                     /pseudo
     CDS             1064..1282
                     /locus_tag="PLW82_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025749.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
     assembly_gap    1554..1563
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1564..2171
                     /locus_tag="PLW82_04550"
     CDS             <1564..2171
                     /locus_tag="PLW82_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOJ46577.1"
                     /translation="LQDHRKGVKAVLKRLQLLGLVLLLAALACAWTDANMVVNGDMAM
                     DWDKNGLPDYWSITWLFSGGPNKPSMDHNENIDGNASLRLDVTSLDVANNFAGRVYQD
                     IPVTSGKTYEISMWVKANWKTADGQSPAGINISYWLQGTNKQWGNREDLTDLVDGTFN
                     WREIKATITIPPKTQAIRLALFAYKGTGTIWIDSIKMVEVK"
     gene            2220..2753
                     /gene="thpR"
                     /locus_tag="PLW82_04555"
     CDS             2220..2753
                     /gene="thpR"
                     /locus_tag="PLW82_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOJ46578.1"
                     /translation="MRLFLAVKVAPDVCRELSSWADEVSLPGKRVAPENLHFTLNFLG
                     EGDPDQVASRLHGAFAGMEPFSVSLKGLGAFFSRGAPRVIFVKVDRGYNELIALEKVI
                     SGKLPEFRKDKPFIPHLTVSRLKEGSGAKEEVEKLIETYKKRDFGSFEVDRVILFSSN
                     LQPAGPVYTVQAQWQLG"
     gene            2815..3111
                     /locus_tag="PLW82_04560"
                     /pseudo
     CDS             2815..3111
                     /locus_tag="PLW82_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
BASE COUNT          995 a          789 c         1010 g          772 t
ORIGIN      
        1 cgatgaaaag aagatcgagc tggcaaaaaa ggccggtgtg gacttggcag gccacgctaa
       61 agagatcggc gccgatgcca tcaagagttt tttgggtcag ggcgtcgatg cagtggtgat
      121 gaccgcaggc agcagctccg aaagcctcgt aaacgagacg cttaaagcgc tcagacctgg
      181 cggaaggtac attatggtcg gcgtatctcc tctcaacatc aacagggaga tcatgttcca
      241 gctagaaagt gaagttatgg taacaagggc aggcggtccg ggccggtacg atcgccagta
      301 cgagctgcac gggaccgact atccgctgca gtacgttcgc tggactgaag gaaggaatat
      361 gcaggagttc atcaggctct tggaaaaggg gagaatcagc accaaggaac tgattaccca
      421 cagatatcac ttcgaagatg ccgcaaaggc ctacgagatg atccaaagcg gcaaggaaga
      481 gtatatcgga gtcatactga catattgatg taatgactga attaaaggaa tgagggttct
      541 ttatggaaaa aggtgcaagg aaaatcgtga gaacacccaa aactcctgga aagatcagcg
      601 ttggtgataa agcagaggtt atcatgggaa ccgatgtcta caactatctc atggagagta
      661 cagacggcgt gccggtaggt aaagatctac acccaatgac caccttccca tgtggccttg
      721 ccatcgacgc tgcaacggaa gccacttcct acgatcttac tggagtaggc acgttgaaag
      781 ccatgagctt cgacctggaa aaccccattc agcccgaagg cgagctagtg ggcagtgccg
      841 aggtagttct gatcgggcgt aggcatgttg aggtagccgt ggaagtggtg caaaaccaaa
      901 gggtgattct taacggccgg gtgataatgg ttaaggtcag agacggccga gcccaggagc
      961 taggcgacct ggtcggttag gtttttccaa cgttagaaga aataaagtag catcaatcgc
     1021 tacctcccgg gcagaataaa tgatgccagg aggtgatatc aagatgggtg caggaagcag
     1081 ttcttctaac aaccctgtgg ttcttggtgc aaaagcagcg ttggaccagt tcaagtacga
     1141 agtggccaat gaacttaata tcgacaccac ccagattcaa aacggttact ggggttacat
     1201 gacatctcgc gattgcggtg ctgttggtgg caacatggta aggcgcatga ttcagcttgc
     1261 tgaagagcag ctcttgtcgc agtatcaaag cgggagaacg ccaagcgtag gcactcctta
     1321 caaacaacag ttggcgcaag accttaatca gtacgccaac gcagcaaaca gacaaggtcc
     1381 aaacgctaac cagatcatga accagataac taactagaag ctccaggccc cgcaaggggc
     1441 cttttttaac ggtctaaagg cacaaactaa agcagagttc gcaggggata aaggattaga
     1501 cctctagaag tagaaaatat actaagacat aaagatgtta atggtaaagt agtnnnnnnn
     1561 nnngattgca ggatcataga aaaggggtga aagctgtgct taaaaggctc caactgctgg
     1621 gtttggttct gttgcttgcc gctttggctt gtgcttggac cgatgcaaac atggtggtaa
     1681 acggagacat ggccatggac tgggacaaaa acggtcttcc cgactactgg tccattacct
     1741 ggttgttttc aggcggaccc aacaagcctt ccatggacca taatgagaat atcgacggaa
     1801 acgctagtct caggcttgat gtcacttctt tagatgtggc caacaacttc gcaggaagag
     1861 tttatcagga tattcccgta acgtccggaa agacctacga gatctccatg tgggtgaagg
     1921 ctaactggaa gaccgcagac gggcagtcgc ctgcagggat caacatctcc tactggcttc
     1981 aggggaccaa caagcagtgg ggaaatcgcg aggatctgac agatcttgtg gacggtacct
     2041 tcaactggag ggagatcaag gccaccatta ctattccccc aaaaactcag gcaattagac
     2101 tggctctttt tgcctataag ggaactggta ctatctggat agacagcata aagatggtcg
     2161 aagtgaagtg agcctagtgc tcacttttta caaatacgca aagagcggga gataaaaaca
     2221 tgcgtctgtt tttagctgtc aaagttgcac cggatgtttg ccgggagctt tctagttggg
     2281 cagatgaggt gagtctgccc ggaaagagag tggctccgga aaaccttcat ttcactttaa
     2341 acttcctggg cgaaggcgac ccggaccaag ttgcaagccg ccttcacgga gcttttgctg
     2401 ggatggaacc tttttctgtg agcctgaaag gtcttggggc cttcttcagc aggggtgctc
     2461 cgagggtaat ctttgtcaag gtggacagag gctacaacga gctgatcgct ctggagaagg
     2521 tcatttccgg gaagcttccg gagttcagaa aggacaagcc gtttatccct cacctcactg
     2581 taagccggct caaggaaggc tccggtgcca aagaagaggt ggaaaagctc attgaaactt
     2641 acaaaaagcg ggatttcggc agctttgagg tggatagggt gattttgttt tcaagcaacc
     2701 tgcagcctgc agggccggtc tatacagtcc aagcccaatg gcagctcggt tgattgagat
     2761 aaagcaatgt ggtacactaa aagagaacaa gtgttcgcta aaggaggtta gtgaatgtcg
     2821 gaagttgaca aggctaaaga aaaggctttg caaagtgcca tcacagcaat agagaaggat
     2881 ttcggcaaag gctcgatcat gcgcctaggc gaaaacaccg cgatggatgt agagaccatt
     2941 cctacaggtg cgctgtcttt ggacctggca cttggggttg gcggtctacc tagaggcaga
     3001 gtagttgaga tctacggtcc cgaaagctcg ggaaaaacca cagtggcact gcatgccatc
     3061 gccgaggccc agaagctagg tggagttgca gcgtttattg atgccgaaca cggtacgact
     3121 tgttgctaga tcaaacccag cggcgctgat caaagtctag cgggatcttt ggtcctaact
     3181 gcatcttcga aatggctggg gaagtgccag ccctgtgaca aggttgggtc acacgctcta
     3241 gtaccccgac gtgcggcagt atttcactgt gctgctgtgc gaagttcgga gaggtcggcg
     3301 aaaatcagac ctggaaagcg gttagagaag tagccgggag tccataaagc tcctatgact
     3361 aagaacatga atcctcgttg taaaagctcg aaaggcatcg caggtgcgaa caataagccc
     3421 tgctccttct tgtgaggttc agcctcagct gcgttaagcg tgtttaaagc agttgaatta
     3481 aaccgtcata ggttttctgt ctttgggcca tccaaccgtg ggcgcaaaga cggctgacgg
     3541 gctagagagg aagtctaagg gagccatgca aggata
//
LOCUS       DAOSDA010000157         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16676_length_3566_cov_2.606380, whole genome shotgun sequence.
ACCESSION   DAOSDA010000157 DAOSDA010000000
VERSION     DAOSDA010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16676_length_3566_cov_2.606380"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="PLW82_04565"
     CDS             complement(<1..1770)
                     /locus_tag="PLW82_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ46579.1"
                     /translation="MQSLRRLISTVIVVLLLLSCAFAIDLKDGIAIVFDAKTEKLIGS
                     DAYRKGIEALEATLYDAYVQYDIITSDEVLEGALDYYEAVILLAQSSMSDQEVELYRD
                     YVNSGGHIIATYTTSLYDAKGNLREDFALGDVLNVTLSSIMPLKPLLGAVPIDPEPPF
                     AADTMMVPSSQTLMVKEIGGVEDYILLTRPGGEFTHYITNTIKTDSTVYFAENIFMTE
                     TSDKYGEVLVRLLEEFLGDIEGGFFRLSVRDIQDPLNEGKSWYKTIEREFKVAERNYE
                     EIPEESLKAYEDAQKVYTALRFAERKDSGEKAGSYLYQLNQLTSQMIPGILQTRNAEA
                     RAVWIDYQSFEACKSEEDFREIIRKLARANINMIFPETVYSGATLYKSAVGEQHAVFR
                     DFPFDPLEVVIDEAKKFGIEVHPWVWVFCGGYWGKFGPALETHPEWRELDEAGRPFSN
                     WEYQTAWYNAAHPEVRAYLLDLFREVVTKYDVDGLHIDYIRYNEDGIGHFGYSDYSKE
                     AFFQATGLRLDEIRVGSKEWIAFNTWRENNVTTFVEEVRKMLNSTNPDLLLSAAVVPD
                     PDRSRADVMQNWKHWVDNGYLDFV"
     assembly_gap    1944..1953
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1954..3240)
                     /locus_tag="PLW82_04570"
     CDS             complement(<1954..3240)
                     /locus_tag="PLW82_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HOJ46580.1"
                     /translation="MDFSAANTSLWNIMLQMGLLSGILLVANILRRKIPFVEKTLLPT
                     AVIAGFIALFLRAFDWFPVKLELLEIITYHSIAIGFIALSLQVPEKRSEAESGGLVAA
                     KSGALIVATYLIQGFFGLLITISLAYTLMPNLFKASGILLPMGFGQGPGQANNVGSTY
                     ENLGFVGGQSFGLSIAAAGYLVACVVGVIYLNYLKRKGAFKNLSSRNYSESLDVAYFQ
                     SEKEIPVSESIDRFSIQIALVLFIYFLTYLASLGLTNFLTASAPGLAKSLSPLIWGFN
                     FIIGSFLATLFRGLFKLLQKAKLMTRQYPNNYLLARVSGLAFDYMVIAGIASIEIKDV
                     QDLWLPLVLLCVVGTVVTIFFLQWLCKKIYPSYYYEGLLSMYGMLTGTISSGILLLRE
                     IDPHFRTPAAINLVTGSSFAIFFGAPMLLFIGLAPES"
     gene            complement(3297..>3566)
                     /locus_tag="PLW82_04575"
     CDS             complement(3297..>3566)
                     /locus_tag="PLW82_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46581.1"
                     /translation="RFETRRRTFKVGEGMLYLYKDRLVLEDKSTGKQTAFLLQDITDM
                     AVIQRTLLTFSVKGKEYFEIKSDHPRSPLKYLLLCKALTDMRVMI"
BASE COUNT          907 a          839 c          909 g          901 t
ORIGIN      
        1 cacgaaatcc agatagccgt tgtctaccca gtgcttccag ttctgcatta catcagctct
       61 ggaacggtcg ggatcgggca ctactgcagc ggaaagaaga agatcggggt tggtgctgtt
      121 aagcatcttg cggacttcct caacaaaggt ggttacgttg ttttccctcc aggtgttgaa
      181 ggcaatccac tctttagaac ctactctgat ttcgtccaat ctcaggcctg ttgcctggaa
      241 gaaggcctct ttgctgtagt cggagtagcc aaaatggcca ataccatctt cgttgtacct
      301 aatgtagtcg atgtgaaggc cgtcaacgtc gtatttggtg acaacctcac ggaaaaggtc
      361 caaaagatag gcccttacct caggatgggc agcattgtac caagcggtct ggtactccca
      421 gttggaaaac ggcctgccgg cttcgtctag ctcccgccat tctgggtgcg tttcgagagc
      481 tgggccgaac ttgccccagt aaccaccgca gaacacccaa acccacgggt ggacttcgat
      541 gccaaacttc ttggcttcgt cgatgactac ctcaagggga tcgaagggga agtctctgaa
      601 gactgcatgc tgctctccta cagcactctt gtagagcgtg gcaccgctgt agaccgtttc
      661 gggaaagatc atgttgatgt tggctctagc gagctttctg atgatctccc taaagtcctc
      721 ttcggacttg caggcttcaa agctctggta gtcgatccag actgccctgg cttctgcgtt
      781 tctggtctgt aaaatcccgg ggatcatctg ggatgtgagt tggttcagct ggtagaggta
      841 tgatccggcc ttttcgcctg agtctttgcg ctcggcaaat ctcaaagcgg tgtagacctt
      901 ttgggcatct tcgtaggctt tcagtgactc ttcgggaatc tcttcatagt ttctctctgc
      961 gaccttgaac tcccgttcaa tggttttgta ccacgatttg ccctcgttta gagggtcttg
     1021 aatatccctg acagacaagc ggaagaagcc tccttcaatg tcacccaaaa actcttctaa
     1081 aagacgcacg agaacctcgc cgtacttgtc agaggtctcg gtcataaaga tgttttcggc
     1141 aaagtacaca gtagaatcag ttttaatggt gtttgtgatg tagtgcgtga actccccgcc
     1201 gggacgagta agcaggatgt agtcttccac gccaccgatt tccttgacca tgagcgtttg
     1261 actggaagga accatcattg tgtcggcggc aaaaggcggt tcgggatcta tcgggaccgc
     1321 tcccaaaagt ggcttcagcg gcattataga actcaaggtt acattaagga catcgcctaa
     1381 ggcaaagtct tctcttagat tgcctttggc atcgtagagt gatgttgtgt aggtagcgat
     1441 gatgtgccca cctgagttta cataatctct gtagagctca acttcctggt cggacatgga
     1501 gctctgggca aggaggatca cggcttcgta gtaatccagc gcaccttcta agacttcatc
     1561 gctggtgatg atgtcatact gcacgtaggc gtcgtataaa gtggcttcga gggcctcgat
     1621 gcccttgcgg taggcatctg agcctatcag tttttccgtc tttgcatcaa agacgatggc
     1681 tatgccatct tttaggtcaa tggcaaaagc acaagaaagc agaagtaaaa ccacgataac
     1741 tgtactgatt aatctcctta aagattgcaa gttatcatct ccccctattc agttcctctg
     1801 ttaatctaat tttagctctg ggcgaaatga attccttctg aaggaaagaa aggcaaaaaa
     1861 ataaccccga gtggggttat ttttgctagg ctttgcgtgt tttcttcccg gcttttaaga
     1921 tgaaagcgac caaggccagg aggnnnnnnn nnncgactct ggcgcaaggc cgataaacag
     1981 cagcatcgga gcgccgaaga agatggcaaa gctgcttcct gtaaccagat tgatagctgc
     2041 gggagttctg aagtgggggt cgatttctct gagaagtagg atgcccgaac tgatggttcc
     2101 cgtaagcatg ccgtacatgg aaaggagacc ttcgtagtag tagctaggat aaatcttttt
     2161 gcacagccac tgcaggaaaa atatcgtgac aactgtgcct acgacacaca agaggactaa
     2221 aggcaaccac agatcttgaa cgtctttgat ctcgatagaa gcaatacctg caatgaccat
     2281 gtagtcaaaa gctaatcccg aaacccttgc taagaggtag ttattgggat actggcgggt
     2341 catcaactta gctttttgca acagcttgaa aagtcctcta aagagggtgg caaggaagct
     2401 gccgatgatg aagttgaagc cccagatgag aggtgacagg ctctttgcaa gtcctggtgc
     2461 tgaagctgtc agaaagttgg tcagacccag cgatgccaaa taggtgagga agtaaatgaa
     2521 aagtaccaaa gcgatctgaa tcgagaagcg gtcgatggat tcggagaccg gaatctcttt
     2581 ttcagactgg aaatacgcta cgtccaacga ttcggagtag ttccttgacg agaggttttt
     2641 aaacgcccct tttcgcttca ggtagttgag gtagatcacc ccaactacgc aggccacaag
     2701 ataaccagct gcagcaatgg aaaggccgaa ggattgccca ccgacaaaac cgaggttttc
     2761 gtaggtggaa cctacgttgt ttgcctgtcc cggcccctga ccgaagccca taggcaggag
     2821 gatgcccgat gctttgaaca agttgggcat caaagtgtag gctaaagaga tggtgatcag
     2881 aagtccgaaa aagccttgaa tcagatatgt tgcaacgatc agagcaccgc tttttgcagc
     2941 taccagacct ccgctttccg cttcggatcg cttttctggc acttgcaggc tcagcgcgat
     3001 gaagccgatg gcaatggaat gataggtgat gatctccaac agctccagct ttacagggaa
     3061 ccaatcaaaa gctctcagga acagagctat aaaacctgca ataactgctg tgggtaggag
     3121 cgttttttcc acgaagggga tttttcgtct caggatgttt gccacaagca gaatcccgga
     3181 cagaagcccc atctgcagca taatgttcca aagactggtg ttagcagctg aaaagtccat
     3241 gtttcgaatc ctcctaatct aaggctttag acatagagcg tgcaaacaac gacggactaa
     3301 atcatcacgc gcatatcggt cagagctttg cagagcaaaa ggtatttcaa cggggatcgg
     3361 ggatggtctg acttaatctc aaagtattct ttccccttta ccgagaaagt taaaagtgtt
     3421 ctttggataa ctgccatgtc ggtaatgtcc tggagcagaa aagccgtttg tttgccggtg
     3481 gacttgtctt ccagaaccag tcggtctttg taaaggtaaa gcatgccttc gccgactttg
     3541 aaggttcgcc ttcgggtttc gaaccg
//
LOCUS       DAOSDA010000158         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16677_length_3566_cov_2.538023, whole genome shotgun sequence.
ACCESSION   DAOSDA010000158 DAOSDA010000000
VERSION     DAOSDA010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16677_length_3566_cov_2.538023"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..621
                     /locus_tag="PLW82_04580"
     CDS             181..621
                     /locus_tag="PLW82_04580"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOJ46582.1"
                     /translation="MKIIRCVTLMNVELITKAEAIARNAEVASIANVDDKGYPRVSTI
                     SNLKTDGLRKVWFATGLGSSKVKFFRKDSKASVCYRDGGNNVTLVGDVEIITSPELKE
                     QLWQDWFIDHFPGGVQDPNYCILQFTTKEVFLWVDNQYEELLFQ"
     gene            719..1369
                     /locus_tag="PLW82_04585"
     CDS             719..1369
                     /locus_tag="PLW82_04585"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ46583.1"
                     /translation="MSDIVREWYEDAENIDEMLDWNKRKLKKWEEKVVSLLPEGAQIL
                     DVGCGMGREAFALTDMGFSVVGIDISEAAVEQVSKLALQKGYHIPFFHYDGHHLPFQD
                     EAFDVVIIWAQTFGLLYGDSYKSSFLKECKRVLKENGLISFSGHDYEYLKDHYRAQLV
                     ERRFYPYPGAEIYWEAFLPQELSKHAEEAGFSVVLCERGEIYKREDGVVLHCLARN"
     gene            1408..1671
                     /locus_tag="PLW82_04590"
     CDS             1408..1671
                     /locus_tag="PLW82_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOJ46584.1"
                     /translation="MPRLSELPNIGPELEKQLMAVGINTPEELREVGSCEAWLRILRQ
                     DPSACMMRLSALEGAIQGVRWHHLDAPTKASLKEFYYKHKSTK"
     gene            complement(1834..2691)
                     /locus_tag="PLW82_04595"
     CDS             complement(1834..2691)
                     /locus_tag="PLW82_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46585.1"
                     /translation="MKRVVSLLVLLTVLVLPACAWENFLEVIVGQTARGATLSPDEKT
                     IYVGAILDRKMVAFDARTGDVIADVLLTNYNSAAWAKGGWVDERGDVFVPATGALEIY
                     HFDSELFHIDTYYIDGFGIENCEGVVTDSHGNLYAFDRKGSGGVYKIDKNGNLDTKWG
                     SRGWADVGFVRLGFLKDGYIYAVDNSSSTLFKVDVETGKVTRLATLASGGFAVAVDDS
                     GKIYVAHYNDPYVAVSIYEDGKVSEISRSEFGIVSNIGGIAVTRDGSKLFLIEEQTDI
                     GGLLLGFKK"
     gene            complement(2905..>3566)
                     /locus_tag="PLW82_04600"
     CDS             complement(2905..>3566)
                     /locus_tag="PLW82_04600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ46586.1"
                     /translation="LEKQNAINSRKDDSAIWFTKELAAELVRKAFLPMLLVSMVNGII
                     RNAVAFWIPTFLSERLGFSPALSASISGGLPLVNLAGTFLGLVILKKLRGNEFRTIGI
                     LFGASTAAFTLAILFSKLSALLVVFMLFVACGAMSSACNLIFASYCLRFKDTGRMSAV
                     TGLLNFSAYAASALASAGFGAVISSFGWSPLTLSWVLLSLVGAWSAFISEKVFFRKKV
                     H"
BASE COUNT          942 a          819 c          950 g          855 t
ORIGIN      
        1 atttggcatg aatgagagtt tcaccggttc ttggtgtatc tttccttaaa cgttggcttg
       61 aaaagggttg cttggaacgc gcttaaggcc tcgatgagcc gggctgcaag tcagacttac
      121 aagctctgat ggggtgacat ttcagcctga cggttatgct tcgatggtgc cgcggaaaaa
      181 atgaaaatta taaggtgcgt gacgctgatg aacgtcgaac tgattaccaa agcagaggcg
      241 attgccagaa atgcggaagt cgcttcaatt gccaatgtcg acgacaaagg atatccgaga
      301 gtctcgacga tctcaaacct caagaccgat ggtttgagaa aggtgtggtt tgccaccggc
      361 cttggatcta gtaaggtgaa gttttttaga aaggacagta aggcgagtgt ttgctaccgc
      421 gacggcggaa acaacgtcac gcttgtcggc gacgttgaga tcatcacttc tcctgaactt
      481 aaagaacaac tgtggcagga ttggttcatc gaccatttcc caggcggcgt tcaggacccg
      541 aactactgca ttttgcagtt caccaccaaa gaagtctttc tatgggtaga taaccaatac
      601 gaggagctgc tgtttcagta gggagaaggt tccttaattg ctcgtgtccg tgaagttgtg
      661 cgatgggcgg acggtcactt gaggttagag aggctagggc caacaagtga ggtgtagtat
      721 gagcgatatt gtacgcgagt ggtatgagga tgccgaaaac atcgatgaga tgcttgactg
      781 gaacaaacga aaactgaaaa agtgggaaga aaaagtcgtc tccttgttgc ctgagggagc
      841 gcagatcctc gatgtgggct gcggtatggg gagagaggct tttgctttaa ccgacatggg
      901 tttttcggta gtggggattg atatttcaga ggcagccgtc gagcaggttt cgaagttggc
      961 attgcaaaaa ggctaccata tcccgttttt tcattacgat gggcatcact tgccgtttca
     1021 agacgaagcc tttgacgtgg tgattatctg ggcgcaaacc ttcgggttgc tttacggaga
     1081 tagctacaaa agcagtttcc ttaaggaatg caagcgcgtt ttaaaagaga acgggttgat
     1141 cagtttttcc ggacacgact atgagtacct aaaggatcat taccgcgctc agttggtaga
     1201 aagacggttt tacccatatc ctggagccga aatctattgg gaagcgtttc tccctcaaga
     1261 actctcgaaa catgcagaag aggccggttt ttcggttgtt ctctgcgaac ggggagagat
     1321 ttacaaacgc gaagacgggg ttgtactgca ctgtttggca agaaactaaa cgtccttttg
     1381 atgttgagga gaaggagggg gtggctaatg cccagactgt ccgaacttcc gaacatcgga
     1441 cccgaactgg aaaagcagct tatggctgtt ggcatcaata cacctgaaga actgagagaa
     1501 gttggcagct gtgaagcttg gctgcgtatt ctaaggcaag acccgtctgc ctgtatgatg
     1561 cggttgtcag cgctagaagg cgcaattcag ggcgttaggt ggcaccattt ggatgcgcca
     1621 accaaagcct cgctcaagga gttctactac aagcacaaaa gtacgaagta gcagagaaaa
     1681 gcggagaagt cactatcata tcagctctcc tcgtggttgt tgacattttt aggaagcttt
     1741 ttgctcgtgc ataggtaaaa aaaggtgggc gtgattttgg tcacgtccac ctttttctta
     1801 aacaaccgaa aaccgtctcg gaaaccttga cgtctacttc ttaaacccta agagaagccc
     1861 gcctatgtca gtctgctctt cgatgaggaa cagttttgag ccgtcgcggg ttacggcgat
     1921 gcctccgatg ttggaaacaa tgccgaactc agaacgggat atttcactga ctttgccgtc
     1981 ttcgtagatg gaaacagcta catagggatc gttgtagtga gccacataga tctttcctga
     2041 atcatccact gccactgcaa atccgcctga agccagcgtg gccagacgcg taaccttgcc
     2101 ggtttcaacg tccactttga acagagtgga agaggagtta tctacggcgt agatataccc
     2161 gtctttcagg aaaccgaggc gtacaaatcc tacatcggcc cagcctctgc tgccccactt
     2221 ggtgtcgaga ttgccgttct tgtcgatttt gtagacacca cctgaaccct tgcggtcaaa
     2281 cgcatagagg ttgccatgac tgtctgttac tacaccttcg cagttttcga tgccaaagcc
     2341 gtcgatgtag tatgtgtcaa tatggaataa ctcagagtcg aagtgataga tctccagcgc
     2401 accggtggcg ggaacgaaaa cgtccccacg ctcatcgacc cagccgcctt tggcccacgc
     2461 tgcagagttg tagttcgtaa gaagcacatc agcgatgaca tccccggttc tggcatcgaa
     2521 cgccaccatc ttccggtcga gaatggcgcc tacatagatg gttttttcat cggggctgag
     2581 tgtggcgcct cttgccgttt gtcctacgat gacctcaagg aagttctccc acgcacaagc
     2641 gggaaggacc aacaccgtta aaagaaccaa cagcgaaacg actcttttca tacgaggacc
     2701 tcctcctcag tgttgtgctg atttagtgtt cgcgggtatt ttgcctaatc cttgcaacaa
     2761 aggcaacttt ttttgaaaaa tatctgccaa tagagaaaat agacgcagac ggagaaaagt
     2821 cacttcggct ggtgcttcac cgcaagcaaa aatccccttc agaccaccta agcgatgtga
     2881 aggggaagtg ttttgctcga agaattaatg cacttttttg cggaaaaata ctttttccga
     2941 tatgaaggcg gaccaagccc cgactaaaga cagaagcacc caagaaaggg taagggggct
     3001 ccagccaaag ctggagataa cagcgccaaa gcctgcacta gccagagctg atgctgcata
     3061 ggcgctgaaa ttgaggagcc cggttactgc agacatccgt ccggtatctt tgaacctcag
     3121 gcagtaggaa gcaaagatca agttgcaagc gctggacatg gcgccacagg caacaaacag
     3181 catgaagacg accagcagtg cactcagctt gctgaagaga atggctaggg taaatgcagc
     3241 agtagatgct ccgaacaaga tgccgatggt cctgaactcg tttccccgga gctttttcag
     3301 gatcacgagc cccagaaagg tccctgcaag gtttaccagg ggaagccctc cggagatgct
     3361 tgcagaaagt gcaggagaaa agccgagtct ctctgacaga aacgtcggga tccaaaaggc
     3421 taccgcattt ctgataatgc cgtttaccat ggaaaccaaa agcatgggaa gaaaggcttt
     3481 tcttaccagc tctgctgcga gctcttttgt gaaccagatc gcagagtcgt ctttcctgga
     3541 attgatggca ttttgctttt ccaggt
//
LOCUS       DAOSDA010000159         3559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16718_length_3559_cov_3.396689, whole genome shotgun sequence.
ACCESSION   DAOSDA010000159 DAOSDA010000000
VERSION     DAOSDA010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16718_length_3559_cov_3.396689"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /gene="dnaG"
                     /locus_tag="PLW82_04605"
     CDS             complement(<1..971)
                     /gene="dnaG"
                     /locus_tag="PLW82_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOJ46587.1"
                     /translation="MDRETATQEIKARLDLAEVVGRYVSLERAGQNYKGLCPFHQEKT
                     PSFTVNPGKQFYYCFGCHATGDVFSFVMHMEGWDFNQALDNLAKEVGITLAGKREENP
                     NNNLLAVMEAVCQYYQSNLKGSPAEKYLQKRGVTLESIKRFRLGYAPPGWENLQAAGI
                     KATEKELLECGLIQKRRKGEGFYDRFRNRVIFPIFNTQGKVVAFGARALGEDKPKYLN
                     SPEGALFQKKEVLYGWHLSKDAIRNLKTIVLVEGYMDLISCYEAGVENAVASMGTALT
                     EEQIRIFARQVDRVVLAYDADAAGKMANRRSVAMLLKNDLQVMEAEFR"
     gene            complement(1039..2046)
                     /locus_tag="PLW82_04610"
     CDS             complement(1039..2046)
                     /locus_tag="PLW82_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOJ46588.1"
                     /translation="MDIRKTLEKREEELLMPLAALSSRSKGREVYEEPCPYRTEFQRD
                     RDRIIHSKAFRRLKDKTQVFISPEGDHYRTRLTHTLEVTQIARTAARALNLNEDLTEA
                     IALGHDLGHTPFGHTGEDALNELVTGGFRHNEQSLRVVEVLERRSSRYSGLNLTYEVR
                     DGILCHTGSRQPATLEGQIVRFADKIAYVNHDAEDAIRAGILKESDLPKEALELGHNS
                     SARINTLVRGLVDYSYGKSQVCLPPELEDKLMKFRQFLFDHVYIGSSAKTEENKAKTL
                     LKMLFRYYMTNPEEILKKTEIEDEMSLERAVADYIAGMTDRFAVHEFSRLFVPEPWNS
                     M"
     gene            complement(2169..>3559)
                     /locus_tag="PLW82_04615"
     CDS             complement(2169..>3559)
                     /locus_tag="PLW82_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005215637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOJ46589.1"
                     /translation="EAEEWAEKGEKVILVRVETSPEDIGGMASAQGILTARGGMTSHA
                     AVVARGMGKCCVSGAGDVKVDFAKKLFKVGDTVVKEGDWISLNGTTGEVFLGQADLIE
                     PQVTGNFATLMSWADKYRQMGVRTNADTPEDAQVARDFGAEGIGLCRTEHMFFKGDRI
                     ISIRKMILADTEEERRAALAELLPYQKDDFTELFKVMKGLPVTIRLLDPPLHEFVPHT
                     QAEQEQMAKEMGRTIEDVKAKVQSLEEFNPMLGHRGCRLAVTYPEIYEMQARAIMEAA
                     CELNKEGYNIVPEIMIPLVGEVKELKYVKDKVEKVVKDTMADYGVSFDYHIGTMIEIP
                     RACLTADEIAKEADFFSFGTNDLTQMTFGFSRDDAGSFIKDYIGKEILTTDVFATLDQ
                     TGVGALVEMAVEKGKSTNPGIKLGICGEHGGDPTTVEFCHKVGLTYVSCSPFRVPIAR
                     LAAAQAAVKNPR"
BASE COUNT          771 a          946 c          843 g          999 t
ORIGIN      
        1 cgaaattctg cttccattac ctggagatcg tttttgagga gcattgccac acttcttctg
       61 tttgccatct tccctgctgc atcggcgtcg tatgccagaa ccactcgatc tacctgtctg
      121 gcaaaaatcc tgatctgctc ctcggtgaga gctgtaccca ttgatgctac ggcgttttcc
      181 acgccagctt catagcagct gatcagatcc atgtatcctt cgaccaaaac tatggtcttc
      241 agattgcgaa tagcatcttt gctcaagtgc cagccgtaga gcacctcctt cttctgaaag
      301 agtgcccctt caggcgagtt caaatacttg ggcttgtctt ctccaagggc cctcgcacca
      361 aacgccacga ctttgccctg agtgttaaag atgggaaaga taactcggtt gcgaaagcga
      421 tcgtaaaacc cttcgccctt tcgccttttc tgtattaaac cacattccaa taattccttc
      481 tctgtggctt ttatacctgc cgcttgcaag ttttcccatc ctggaggtgc gtagccaagg
      541 cgaaacctct tgatgctctc cagagtcaca cctcgctttt gcaggtattt ttctgctgga
      601 gaccctttga ggttagactg atagtactga cagaccgcct ccataacagc caggaggtta
      661 ttattcgggt tttcctcccg ttttcctgct aaagttatgc caacttcttt cgccaagttg
      721 tctaaagctt ggttgaagtc ccagccttcc atatgcatga cgaagctgaa aacgtcacca
      781 gtggcatgac agccgaagca gtagtaaaac tgcttgccgg ggtttacggt aaatgacggt
      841 gttttctctt gatgaaaagg gcagagtcct ttatagtttt gccccgcgcg ctccaaagag
      901 acgtaacggc caacgacctc tgcaagatcc agtctggctt tgatctcttg agtcgcagtc
      961 tccctatcca tcgagatcac ctcatatatc cttcattttc tctagttttc aaattattcc
     1021 ttcccaagaa cttcaagcct acatactatt ccacggttcg ggaacaaaga gtctggaaaa
     1081 ctcgtgaact gcaaaccggt cggtcatccc ggcgatatag tctgcaactg ccctctccag
     1141 agacatttcg tcttcgatct cggtcttttt caagatctct tctgggtttg tcatgtaata
     1201 cctaaagagc atcttgagta gggtcttggc cttgttctcc tccgtctttg ctgaagagcc
     1261 aatatagacg tggtcgaaca aaaactggcg gaacttcatg agcttgtcct caagttcggg
     1321 aggtagacac acctggctct tgccgtagct gtagtccaca agacctctta ccagtgtgtt
     1381 gatccttgcc gatgagttat ggcccagctc taaagcctct ttgggcagat cgctttcttt
     1441 aagaatcccc gcgcgaattg cgtcttctgc gtcgtgattc acgtaggcga ttttgtctgc
     1501 aaatctaaca atctgacctt ccagcgtcgc tggctgcctg ctgccggtgt ggcacagaat
     1561 gccatctctt acctcatagg tgaggttcag cccactgtag cgggagcttc ggcgttctag
     1621 aacctctaca actcgaaggc tctgctcgtt gtgcctaaaa ccgccagtca ccagctcatt
     1681 tagggcgtct tcaccggtat gtccgaaggg tgtatgcccc aaatcgtgcc cgagagcgat
     1741 ggcttcggtg aggtcttcgt ttaggttcag cgctcttgcc gcagtcctgg caatctgagt
     1801 gacttctaag gtgtgagtaa gacgagtgcg ataatgatcg ccctcaggag aaataaagac
     1861 ctgagtcttg tctttcagcc tccggaaggc ttttgaatgg attatgcggt cgcgatctct
     1921 ctgaaactcg gttctgtacg ggcagggttc ttcgtagact tccctcccct tgctcctgct
     1981 actcagtgca gctagaggca taaggagctc ttcttcccgt ttctctagag ttttgcgaat
     2041 atccattgaa ttctcaccac ctcagtttta cagttcatca gagtggtggg aacttccttc
     2101 ttggcggccg aaggcaaaaa agagaagggc aaagacgttt gtctctgccc catcagaatc
     2161 tagtttactt accttggatt cttcacagca gcttgagcag cagctagtct tgcaattggt
     2221 acgcggaaag gcgaacagct tacataggtg aggccaactt tgtggcagaa ctcaacagtc
     2281 gttggatcgc cgccgtgctc accgcagata cccagcttga tgcctgggtt ggtgctctta
     2341 cctttctcaa cggccatctc aaccaaggcg cctacaccgg tttgatccag agttgcgaat
     2401 acgtctgtgg ttagaatctc tttgccgata tagtccttga tgaaggagcc tgcgtcgtcg
     2461 cggctgaagc cgaaggtcat ctgggtaagg tcgttggtac cgaaggagaa gaagtctgct
     2521 tcttttgcga tctcatcggc tgtgagacat gctcttggaa tctcgatcat ggtaccgata
     2581 tggtagtcga aggacacacc gtaatctgcc atggtgtctt tgaccacttt ctcgacctta
     2641 tctttgacgt acttgagctc ttttacctcg cctacgagag gaatcatgat ctcaggaacg
     2701 atgttgtaac cttccttatt caattcgcaa gctgcttcca tgatggctct agcctgcatt
     2761 tcgtagatct ctggataagt tacagccaga cggcagccgc gatgaccgag cattgggttg
     2821 aactcttcga ggctttgtac tttggcttta acatcctcaa ttgtgcgtcc catttctttg
     2881 gccatctgtt cttgctcagc ctgtgtgtga ggcacaaatt cgtggagagg tgggtccaac
     2941 agacgaatgg tcacaggtag acccttcata accttgaaca gctctgtaaa gtcgtccttc
     3001 tgatatggca gaagctcggc cagagcagcg cggcgctctt cttcggtgtc ggcaaggatc
     3061 atcttgcgga tggagatgat tctgtcgcct ttgaagaaca tgtgctctgt acggcacaga
     3121 ccaatacctt ctgctccgaa gtctctagca acttgagcat cttctggagt atcggcgttg
     3181 gttcttacgc ccatctgacg gtacttgtct gcccagctca tcaaggtggc aaagttgccg
     3241 gttacttgag gctcgataag gtctgcctga cccaggaaga cttcaccggt ggtaccgttc
     3301 aaggagatcc agtcgccttc tttgactact gtgtcgccga ctttaaagag cttcttggcg
     3361 aagtcgacct taacatctcc ggcaccggat acgcagcact tacccatacc gcgagcaaca
     3421 actgctgcgt gggaggtcat gccgcctctg gcagtgagaa taccttgagc agatgccatg
     3481 ccgccgatat cctctggtga agtttcgact ctaacgagaa tgaccttctc gcctttttct
     3541 gcccactctt cagcttctt
//
LOCUS       DAOSDA010000160         3540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16846_length_3540_cov_2.819512, whole genome shotgun sequence.
ACCESSION   DAOSDA010000160 DAOSDA010000000
VERSION     DAOSDA010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16846_length_3540_cov_2.819512"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="PLW82_04620"
     CDS             complement(<1..1533)
                     /locus_tag="PLW82_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46590.1"
                     /translation="MSRILFRTLAVALILSLASAVISAEEMWTSVVSLYIYDGERPVF
                     ADHFFEVLDQGEYILLPLTQLANYLELNLEYSSLENVISVSSDRGSVRISLEDNLYLD
                     HPEWSTYEPTTFEDVFFVAPQVFEYVAGVSIEWQPLYQTVVVTLEELPNWLKSSQTDE
                     QKAAKTDEEFILAKSKVRNGLTLFRYQFSSLFEYPAKKHTLSADWEVFGKVGPFSIHA
                     IVDTDLINYAQHSSELKFIRAKHEAGNHLLVLGDHEAVWEASLGKVVVRGLYFQYARG
                     NTTTNIAYKNFSGEVIPGSTVTLYVNNQLVASLSMSETVSGTYEFLRVPLRANQLNYV
                     RIVITKPTGEFEERHYQVAGEPRIFLPGTLMLQTAHGLYRPVTGALFQNRTHTVSLLA
                     GSGSSSFFLDWHWLEKPNANPEDAFRLSLYGQPYGNMILNANAYGSLLNREEKISGSL
                     NLKLYYASVIGSFKVYDVASDVSSIFPQRAGRGTTFALTYYPLTNWSIELLLADNEKD
                     AVS"
     gene            complement(1559..2326)
                     /locus_tag="PLW82_04625"
     CDS             complement(1559..2326)
                     /locus_tag="PLW82_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46591.1"
                     /translation="MKKLAIVLLVFTTILTATASIQVEPAVFELFLNDEERITEAIRV
                     TNFSQLPLTISVSAYDWYISESGEFAEARLGSLDSTLQGHLRFNPRQFALLPGQTQIV
                     RVTIDMPSEPPLQRRSVIFFSTEQTSYTGQIQTVIQTVVGATLYVTNRRAKPSFTVHG
                     LDYIWSDSNLVLNVITENTGDIHFRTSITYDIYDLGGQLLYSDAFSNIVILPGQKHTY
                     SLPVKAAIVPGLYKVVGKITNTTTQNVVPFSYEIAVN"
     gene            complement(2353..2949)
                     /locus_tag="PLW82_04630"
     CDS             complement(2353..2949)
                     /locus_tag="PLW82_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46592.1"
                     /translation="MKRVLKAFLLISVLLMGSSLGFADEPATVGVTIDATATIPTFVR
                     FTLGDWSAMINPGLGVPGVQYSSSPSLNFSHSTGYMDGSWKLLSATAEPDQENVYIPS
                     NTLTAAIVANCKYTYYITGSSLTYQGDPEATSLPIEFMYEIDGTKSDTKSLNGEHEIL
                     TNDAPGRKEITHRFRVPYSLEYLAGTYKGQVTFKVVVM"
BASE COUNT          939 a          772 c          874 g          955 t
ORIGIN      
        1 cgaaacggcg tccttttcgt tatctgccag aagcagctct atcgaccaat tggtcagagg
       61 atagtaggtg agggcgaagg tggtgccgcg tccggctctt tgcgggaaga tagacgaaac
      121 atcgctggcc acatcgtaga ccttgaaaga cccgataacg ctggcgtaat acagcttcaa
      181 attcaatgat ccgcttatct tctcttcacg gttcaggagc gaaccgtagg cattagcgtt
      241 taggatcata ttcccgtagg gttgcccgta gaggctgagt ctgaaagcat cttcaggatt
      301 tgcattaggt ttttccagcc agtgccagtc cagaaagaag ctagaactcc ccgaacctgc
      361 cagaagcgat acagtatggg tgcgattttg aaacagcgct cctgtaacag gacggtagag
      421 accgtgggcg gtctggagca taagggttcc cggaagaaag atccggggct cacctgccac
      481 ttggtagtgc cgttcttcaa actctcctgt gggtttggtg atgacgatgc ggacgtagtt
      541 cagctgatta gccctcagcg gtacacggag aaactcgtaa gttccgctta cggtctcgct
      601 catgctgagg cttgcgacca gttggttgtt tacgtacaag gtgactgtcg agcctggaat
      661 gacctcaccg ctgaaatttt tataggcaat gttagtggta gtgtttcctc ttgcgtactg
      721 gaagtagagc cctctaacaa ctactttgcc cagggaggct tcccaaacgg cctcgtggtc
      781 gcctaagacc aagagatggt tccctgcttc gtgctttgcc ctgatgaact tcagctccga
      841 ggagtgttga gcgtagttta ttagatccgt atccacgatg gcgtggatgc tgaatggtcc
      901 gactttgccg aagacttccc aatctgcact caaggtgtgc tttttggcag gatattcaaa
      961 gagcgagctg aactggtagc gaaacaaagt gagaccgttt ctcaccttgg actttgccaa
     1021 aataaactct tcatctgttt ttgcagcttt ctgctcgtcg gtctgggagg atttaagcca
     1081 gttagggagt tcttccaacg tgactactac agtttgatac agaggctgcc actcgatgga
     1141 aacgccagct acatattcaa acacttgtgg agcaacaaaa aaaacgtcct caaaggtagt
     1201 tggttcgtat gtactccatt caggatgatc taggtagagg ttatcctcta gggaaatccg
     1261 cacggaacct ctgtccgaac ttaccgagat gacgttttcc aaactgctgt attcaagatt
     1321 taactcaaga tagttggcca gttgagtaag gggcagtaga atgtactctc cttggtctag
     1381 aacctcaaaa aaatggtctg caaagaccgg tctttcgccg tcgtaaatgt acagacttac
     1441 tacacttgtc cacatctctt cggctgagat cactgcagaa gcaagggata aaatcagtgc
     1501 aactgcaagt gtgcgaaaca gtattctgct catcttgctt ctacagtacc tccttcgatt
     1561 aattaacagc tatttcatag gaaaagggaa cgacattctg agtggtggtg ttggtgattt
     1621 tacccaccac tttgtaaagg ccgggaacga tggcagcctt tacaggaagg ctgtatgtgt
     1681 gcttctgtcc tggcaggatg actatgttgc tgaacgcatc ggagtagagg agctgaccac
     1741 ctagatcgta gatgtcgtag gtgatgctgg tgcgaaaatg gatgtcgccg gtgttttctg
     1801 taatgacatt aagcactaga ttactgtcgc tccagatgta gtccagacca tggacagtaa
     1861 aagatggttt ggcccgtcta ttggtgacgt acaaagttgc tccaactacc gtctgaatca
     1921 ccgtttggat ctgcccggtg tatgaagtct gttctgtgga aaaaaagatc actgaccgcc
     1981 tctgaagcgg aggttcgctg ggcatatcga tggtcacccg gacgatttgg gtctgccctg
     2041 ggagcagggc aaactgccta gggttgaacc tgagatgccc ctgaagggtt gaatctaaag
     2101 agccgagtct tgcttcggca aactctccgc tttcgcttat ataccagtcg taagcgctta
     2161 cagaaatggt aagaggcaat tgagagaagt ttgttactct aattgcctct gtaatacgct
     2221 cttcgtcatt caagaacaac tcaaaaacag ctggttctac ttggatagag gcagtagcag
     2281 tcagtatcgt agtaaaaaca aggagcacaa tcgctagctt tttcattact ccgcctccag
     2341 gttatgtcga gtttacatta cgactacttt aaaggtgacc tgtcctttat acgtaccggc
     2401 cagatactcc aggctgtaag gaacgcggaa cctgtgtgtg atctcttttc ggccaggtgc
     2461 gtcgttggtt aaaatctcat gctcgccgtt gaggcttttg gtatcggact ttgttccgtc
     2521 tatttcatac atgaactcga tggggagaga cgttgcttca gggtcacctt ggtatgttaa
     2581 gctgcttccg gtgatgtaat aagtatattt gcagttggcc acgatagcag ctgttagtgt
     2641 attggaaggg atataaacat tttcctgatc aggttcagca gtcgctgaaa gtaacttcca
     2701 gctgccatcc atgtaacctg tggaatgact gaagttcaag ctggggcttg aggaatattg
     2761 gactccagga actcctagtc ccggattgat catcgcagac caatcgccaa gggtgaatcg
     2821 gacaaaggtc gggatagttg ctgtggcatc gatggtaacc cctactgtcg cgggctcatc
     2881 tgcaaaaccc agggagctac ccattagaag cacagaaatc aagagaaacg ctttcaacac
     2941 tcttttcatt aaaaaacctc cttgaatttg tttagcgaaa accaaaggtt agtatacttt
     3001 gttgtagggc aacttcatct ttataccacg aggagaaaac taccgcaagc ttttcttagt
     3061 aaaggctact ttgactacca aaacctgcta tttaaaaaaa cgccaaaacc aacaacagtt
     3121 aaaaggcacc cccaaaaggc ctcttggtta ggtgttaaaa aagcgattcg agatgattgt
     3181 gcataatatt tgaataataa ccaccagttc gaaagtggta tgtacggcaa ctagataagt
     3241 atgaaattag taatggttat caattctact aatcattcta gtactaaagt cctagggctg
     3301 tcaaggaatt tttgatcagt tttgtgtaat tgtcaaaaat cattcaaaaa tcaatgcaaa
     3361 cgcagagctg ggaaattgtc aggtttttga caatttggga ttttttgcag ggatttgggc
     3421 cacaaaaaaa gcctgagtat cacctgaagc gtttgaaact ttaaacggag atgataacag
     3481 actctggtgt gatcgggata tgaacactag accagcagta cactttttta cggatcctgt
//
LOCUS       DAOSDA010000161         3539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16849_length_3539_cov_4.316016, whole genome shotgun sequence.
ACCESSION   DAOSDA010000161 DAOSDA010000000
VERSION     DAOSDA010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16849_length_3539_cov_4.316016"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLW82_04635"
     CDS             <1..195
                     /locus_tag="PLW82_04635"
                     /inference="COORDINATES: protein motif:HMM:NF027473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4855 domain-containing protein"
                     /protein_id="HOJ46593.1"
                     /translation="RERYYDYLRTAVTLGYENDILRGWYQDVKIFHDAARSTWPEVRE
                     VYDVTYKFVKGEYTETIQFE"
     gene            complement(265..723)
                     /locus_tag="PLW82_04640"
     CDS             complement(265..723)
                     /locus_tag="PLW82_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46594.1"
                     /translation="MSTVEDKLNELEVYLKSAKELPLTDMVLIDPEKVFKLIQEIRDT
                     LPEEIKQAEEVLKESEKIIADAKNKAQNEYLVAVARNEAILKDTDVVREAQSKKEQIL
                     KEAEKEAKFYVDSTLEWIDSKFLEMQKILEQNLEATKLGRSELKRFKIQR"
     gene            complement(737..1216)
                     /gene="coaD"
                     /locus_tag="PLW82_04645"
     CDS             complement(737..1216)
                     /gene="coaD"
                     /locus_tag="PLW82_04645"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOJ46595.1"
                     /translation="MKVAICPGSFDPLTNGHVDIINRAAQVFDKVIVAVLHNPNKKPL
                     FTMEERAHMIKEALKDLPNVEVEYFSGLLVDYARSKGACAIVRGLRAVSDFEIELMQA
                     SVNRKLAPEVETVYMMTSNEYSFLSSSVVKEVASFGGDVRCMVPTVVAEKLAEKFGK"
     gene            complement(1213..1761)
                     /gene="rsmD"
                     /locus_tag="PLW82_04650"
     CDS             complement(1213..1761)
                     /gene="rsmD"
                     /locus_tag="PLW82_04650"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOJ46596.1"
                     /translation="MRIVGGKHRGRTIKGPPKKQQLNTRPTTDRVRESLFNIIAPRIR
                     GANFLDLFAGTGAVGIEALSRGAESLTLVEKNMAMVRVIKDNLLSLGEEAEVFPVDVY
                     KALEALAKEGRKFEIIFADPPYHLETGQEFFQLVEPVLSEGGLLVLEHSAEQIDQNIL
                     LEQKRYVYGETIISLFTRGEHT"
     gene            complement(1793..2650)
                     /locus_tag="PLW82_04655"
     CDS             complement(1793..2650)
                     /locus_tag="PLW82_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOJ46597.1"
                     /translation="MSKTVIVIDSTADLPESLRRELDLHMVPLNVHFGTEVYKDQVDL
                     DSKTFYQKLKASKDVMPRTSQPSPAQFVDLYKQVASPDDRILSIHISSKASGTIQSAL
                     IAKSLLPEYNIEIIDSEGLSIHYGMQAVEMAKLAQQGASVEQILEHRKRVKESIEVCF
                     TVETLEYLHKNGRIGTANAFLGTVLSIKPILSIENGMVSGVEKVRGRKKAVKRLVEYI
                     VDKMIDAPGQKLYLGIVQGDAEEEADGIEAELRAIFPKAESIQRFDMGPIIGSHAGPG
                     TVGIIGYKL"
     gene            complement(2666..>3539)
                     /locus_tag="PLW82_04660"
     CDS             complement(2666..>3539)
                     /locus_tag="PLW82_04660"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOJ46598.1"
                     /translation="IVVGDEDMVKVHVHTNNPGLALEKALKYGQLMKIKIENMVEQNR
                     EAVASGKAAWSANTGAVEFKSTPKQEVEREVGIVTVATGEGITEILRSLGADKIVAGG
                     QTMNPSTADILEAVENCPAKNVIILPNNKNILTTAAQVPSVTDKNVFVIESKTIPQGI
                     KALLSYDPDISPEENVKSMSEAIKEVKTGEVTFAVRDTSMNGFQIEEKDIIGLAEGKI
                     ETVGKDVSTVVKELVKKLVDENSAILTLYFGADVSEEAANELLDELQEEYPDLELEMY
                     NGNQPLYYYLISVE"
BASE COUNT          815 a          900 c          754 g         1070 t
ORIGIN      
        1 agagaacgtt actatgatta cctccggaca gctgtaaccc ttggttacga aaacgatatc
       61 ctgcgcggtt ggtatcagga tgttaagatc ttccacgatg cagccagaag tacctggcca
      121 gaagttcgcg aagtctacga cgtcacttat aagttcgtta agggtgagta tacagagact
      181 atccagtttg aataaaccga tgcaaaacaa aggagagggc ttcacctgcg aagctctctc
      241 cttatttttc gcacgacaat agatctacct ttgaatcttg aaccgcttca gttcagagcg
      301 gcctagcttg gttgcttcaa ggttctgctc caatatcttc tgcatctcca agaacttgct
      361 gtcaatccac tccaaagtgg aatcaacgta gaacttggcc tctttctctg cttctttcaa
      421 aatctgttcc ttcttgcttt gggcctctcg gacaacatcg gtgtccttca aaatggcctc
      481 attacgtgct acagcaacga gatactcatt ttgagccttg tttttggcat cggctatgat
      541 cttttcgctc tctttaagta cttcttcagc ctgcttgatc tcctcaggca gggtgtcgcg
      601 gatttcttga ataagtttga agaccttttc cggatcaatc agaaccatat ctgtcaaggg
      661 aagctctttt gcagacttga gatagacttc aagttcattt aacttatctt caaccgttga
      721 catctggttt ttccccctac ttaccgaatt tttccgcgag tttttcagcc actaccgtgg
      781 gcaccataca tctcacatcg ccgccaaagc tagccacttc cttcaccacg ctggagctga
      841 ggaacgagta ctcattgctg gtcatcatat agaccgtctc tacctccggt gcaagcttgc
      901 ggttcacaga cgcctgcatc agttcgatct caaaatcgga aacagctcgc agtcccctga
      961 cgatggcaca agcgcctttg cttcttgcgt agtccaccag tagtccgcta aagtactcca
     1021 cttccacgtt gggcaggtct tttaaagcct ctttgatcat atgtgccctt tcctccatgg
     1081 tgaaaagagg cttcttgttg ggattgtgca gaacagcaac gataactttg tcaaacacct
     1141 gtgctgctct attgataatg tccacatgac cgttggtcaa aggatcgaaa ctgccagggc
     1201 agattgcaac tttcatgtgt gttccccccg tgtgaacaaa gaaataatcg tttcaccgta
     1261 gacgtatctt ttctgttcca agagaatgtt ctgatcgatc tgttcagcgc tgtgctctaa
     1321 aacaagcaat cctccctcag ataaaacagg ctccaccagc tggaaaaact cctggcctgt
     1381 ttctaaatgg tacggaggat cggcgaagat gatttcgaat ttcctgcctt cttttgctag
     1441 tgcttctaaa gctttgtaga cgtcaacagg gaagacttct gcttcctccc ccaagctaag
     1501 taggttgtct ttaatgaccc tcaccatagc catgtttttc tcaacgagag tcagactctc
     1561 cgcccctcta cttaaagctt caatcccaac tgcaccagta cctgcaaata aatcaaggaa
     1621 atttgctccc cggatgcggg gagcaattat attaaatagc gattcacgta ctctgtcagt
     1681 tgttggtctg gtgttcagct gttgcttttt tggcggtcct ttgattgttc tacctctgtg
     1741 ttttccacca actatacgca tgatctcaat ccattcttca caaaactgga cgttacagct
     1801 tatacccaat gatcccaacg gtgccaggac ctgcgtggga gccgataatc ggtcccatgt
     1861 caaatctctg tatagattcg gctttgggaa aaatcgctct cagttcggct tcgatcccat
     1921 ccgcttcttc ctctgcgtct ccctgaacta tacccaagta cagtttctgt ccaggtgcgt
     1981 cgatcatctt gtccacgatg tactctacaa ggcgtttgac agccttcttt ctgccgcgaa
     2041 ctttctccac acctgaaacc atgccgtttt caatggacag aatcggctta atgctcagta
     2101 ctgtacctaa aaaagcatta gcagtgccga tacggccgtt cttgtgaagg tactccaagg
     2161 tctctacggt aaagcaaacc tcaatgctct ctttgacacg cttgcggtgt tctaggatct
     2221 gttctaccga agctccttgt tgggcgagtt ttgccatttc cactgcttgc attccatagt
     2281 ggatggatag accttcgctg tcaatgattt cgatattgta ctccggaaga agagacttgg
     2341 cgatcagtgc cgactggata gtaccgctgg ctttgctgga aatgtgtatg ctgagaatcc
     2401 tatcatctgg agatgccacc tgtttgtaca gatcaacaaa ctgagcaggg gatggctgag
     2461 acgttctggg cataacatcc ttactcgctt tgagcttctg gtaaaaggtt ttgctgtcta
     2521 gatcaacctg gtctttgtaa acctctgtcc caaaatgcac attaaggggc accatatgca
     2581 gatccagttc tcttctcaga ctctccggca gatcagcggt gctatctata acaataaccg
     2641 ttttgctcat actaggtgca gccccctact ctacagagat caggtagtag tacaaaggtt
     2701 ggttgccgtt gtacatctcc agttccaaat ctggatactc ctcttgcagt tcatccaaca
     2761 gttcgttagc tgcttcttct gaaacatctg caccgaagta tagagttaga atggccgagt
     2821 tttcatcaac aagcttcttg accagttctt tgactacggt actgacgtct ttaccaacag
     2881 tttcaatctt gccttcagca agaccaataa tgtccttttc ttcaatttgg aagccgttca
     2941 tgctggtgtc tctaacggca aaggtgacct cgccggtctt aacttctttg atggcttcgc
     3001 tcattgattt gacgttttct tccggactga tgtcgggatc gtagctgaga agcgctttga
     3061 tgccctgggg tatggtcttg ctttcgatga caaaaacgtt tttgtcagtt acgctgggca
     3121 cctgggctgc tgtggtaagg atgtttttat tgttgggtag aataataacg tttttagcag
     3181 ggcagttctc tacggcttcc aatatgtctg ctgtagaggg attcatcgtc tgcccgcctg
     3241 caactatttt gtctgcacct aaggaacgaa ggatctcagt gattccttca ccagttgcta
     3301 cagtcactat gccgacttct cgttcgactt cttgttttgg agtagatttg aactctacgg
     3361 cgccggtgtt tgcactccag gccgctttgc cgcttgcaac tgcttctctg ttttgctcaa
     3421 ccatgttttc gattttgatc ttcatcagct ggccgtactt tagagccttt tccagtgcca
     3481 gaccggggtt gttggtgtga acgtggactt taaccatatc ttcatcgcca acaacaatc
//
LOCUS       DAOSDA010000162         3534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16892_length_3534_cov_2.995401, whole genome shotgun sequence.
ACCESSION   DAOSDA010000162 DAOSDA010000000
VERSION     DAOSDA010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16892_length_3534_cov_2.995401"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..762)
                     /locus_tag="PLW82_04665"
     CDS             complement(10..762)
                     /locus_tag="PLW82_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ46599.1"
                     /translation="MLELHDIVLERNNVRSLANVNLKVEDGETVVLTGPSGCGKSTLI
                     RVCLGLIKPKSGEVWFEGVNLQALSPEALRKKRRKMGIVFQEFQLLDHFTVLENVLLA
                     LWEGDRDRKKAEEAIKSVGLWDKRFRYPRQLSGGEKQRVGIARALALDPDIIFWDEPT
                     AALDPMYVHEILRIMDSLAQKKTMLVVTHEMPFALKVADRLVLMEAGRIVEEAPPSRI
                     FAEPASHVGAMYRERLLDTREALGKLDVKQVV"
     gene            complement(755..2605)
                     /locus_tag="PLW82_04670"
     CDS             complement(755..2605)
                     /locus_tag="PLW82_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46600.1"
                     /translation="MRGGIKLDLAIVIVLEIVLLSAAILVAGHGFSFYVQRGLDDIIG
                     GTEYQVIVQVDKKETKNFVEGLKEVYSGRDGFKFRVSKELGENIHILLGYSEEDLKNG
                     ALDEVAKLISHLPGYGGLAPLLDPAVLVKGMPQDTYPELVEEIHTWPEVKFAAKGPLG
                     LYVVAKNRRNLNKIEDKLEELTSKYYRWEIELKDYTNQQAAEIREMIDEALKGTKYWF
                     TTPPAEGVIGDAVRLLKSYTAQLTFAKELPVGSEIALTARNLKVGTKADADFKARVYT
                     DGIYLIKGDSLNLTGSEKAYLLDGSGTVKEVLPEYTLESPRRDLDVWEPKADARNLKA
                     SIQSLADKVVQGEDFGILIREIEQLAKNLSLEVPSDLKSQLSVLVLWDRVGRLPGGFR
                     EELAQKLLQWAEAMPSDPLIKLKESLPSWSDEDIAKSWQEIEAIARPKTISVITTGSA
                     PPIDGVISKPAAEISEDIKSQIGGVVNSVVPFAALTVVVILLWVFTAVDHTLWGSYLD
                     HKNNYLAPLYGWLLGVLEMAVVVYIMPFEMSGMVKLIYVAVGGLGSAVLITFSSRISP
                     LNAKSIEAGEALGLSQTELYRQVVVPEGRPGLWQLLNRRKLLSPRRFKNA"
     gene            complement(2731..>3534)
                     /locus_tag="PLW82_04675"
     CDS             complement(2731..>3534)
                     /locus_tag="PLW82_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOJ46601.1"
                     /translation="KGDVAGFIVEPIQGEGGIIVPDKGYLKAAEELCHKYGALLIADE
                     IQTGLGRTGKMFACEWDEAKPDIMCIAKSISGGIMPTSLFITTEEIWMNAYGSKEKCL
                     LHTSTFGGNTWSAAAGIAALEVIVSENLPQRALELGDYLLSELNRLKDKHGLIKEVRG
                     KGLLIGLEFEQPKGFLDKISGGALTRASEEYFGALVAGELQNKHGVITAYTLNNPNVI
                     RLEPPLTVTKEEIDKVINALDIIFTENKSFLKLAVKSSGSIISSVLGRK"
BASE COUNT          807 a          935 c          831 g          961 t
ORIGIN      
        1 agaagcaggt caaacgacct gcttcacatc tagctttcct aaggcctctc tggtgtctaa
       61 gagcctttcc cgatacatag ctccgacgtg agacgcgggc tcggcaaaga tcctgctagg
      121 aggcgcttct tcaacgattc tgcctgcttc catgagtaca agtcgatctg ctactttgag
      181 agcaaacggc atctcatgag ttactaccag catcgttttt ttctgtgcta gcgagtccat
      241 aattctgaga atctcgtgaa cgtacatagg atcaagggca gcggttggtt cgtcccaaaa
      301 gatgatgtcg ggatccaaag ccagggctct agcaatacct accctctgct tctcaccgcc
      361 tgagagctgt ctgggatatc taaacctttt gtcccaaagg cctaccgact tgatggcttc
      421 ttcggccttt ttgcgatctc tgtctccttc ccaaagagct aggagtacgt tttccaggac
      481 ggtgaagtgg tccaggagct ggaactcctg aaaaacgatg cccatcttgc gccttttttt
      541 gcgcagagct tctggcgaca gagcctggag gttaacacct tcaaaccaga cctcgccgga
      601 cttgggtttg atgagcccta aacagactct gatcaaagtg gacttgccgc atccgcttgg
      661 tcctgtgaga acaaccgttt cgccgtcttc caccttgagg tttacgttgg caagagagcg
      721 cacgttattt ctttctaaaa caatgtcgtg aagttcaagc attcttaaac ctccttggac
      781 taagcagctt gcggcggttc aagagctgcc acagaccggg tcttccttcc ggtaccacta
      841 cctgcctgta gagttcggtt tgggaaagac ccaatgcctc tcctgcttca atgctcttgg
      901 cgtttagcgg ggaaatgcgg ctggagaagg tgattagcac agcgctgcca agtccgccta
      961 cggcaacgta aatcagcttg accatgccgg acatctcaaa tggcatgatg tagaccacaa
     1021 cagccatctc taggacgcct aaaagccagc cgtagagagg tgccaagtag ttgtttttgt
     1081 ggtccaagta gcttccccaa agggtgtggt ctactgcagt gaacacccaa agcagaatca
     1141 cgaccactgt cagagctgca aacggcacga cgctgttgac cacgccgccg atctggctct
     1201 taatgtcctc actgatttct gcggcaggtt tgctgatcac tccatcgatg ggcggagcag
     1261 atccggtcgt gatgacgctg atggtcttgg gacgggcaat agcttcgatc tcctgccagc
     1321 tcttagcgat gtcctcatcg ctccaagaag gcaagctctc tttcaacttg atgagaggat
     1381 cggaaggcat ggcttctgcc cactgaagga gcttttgcgc cagctcttct ctgaaacctc
     1441 caggaagcct tcccactctg tcccagagca ccaagacgct gagctgggac ttcaagtccg
     1501 atggaacctc caaactgagg tttttagcca actgttcgat ttctcttatc aagatgccaa
     1561 agtcctcacc ttgcactacc ttgtccgcaa gggactgaat acttgccttc agattcctgg
     1621 cgtctgcttt gggctcccaa acatctaaat cccgtctggg actttctaga gtatattccg
     1681 ggagcacttc cttcacagtt ccgcttccat ctaaaagata ggctttttcc gaacctgtaa
     1741 ggttcaaaga atcgcctttg atgaggtata tgccatctgt ataaacccta gctttgaagt
     1801 ctgcatcggc tttagtgcct actttcaagt ttcttgcggt caaagcaatc tccgaaccca
     1861 ccggcagctc tttagcgaag gtgagttgag ccgtataact tttcaagaga cgcaccgcat
     1921 cgccaataac accttcggca ggcggtgttg taaaccagta tttggtcccc ttaagagctt
     1981 cgtcgatcat ttccctaatc tctgcagcct gttggttggt gtagtctttg agttcaatct
     2041 cccacctgta gtacttggac gtcagttcct ctagcttgtc ttcaatcttg tttaggtttc
     2101 tgcggttttt ggcaacaaca tataggccaa gaggtccttt tgcagcgaat tttacctcag
     2161 gccaggtgtg aatctcttcc acaagttcgg gataggtatc ttggggcata cccttaacca
     2221 gcaccgcagg gtcgagaagg ggtgccaacc cgccgtatcc cggcagatgg cttatgagct
     2281 ttgccacttc atccagtgca ccatttttca aatcttcttc gctgtaacct aagagtatgt
     2341 gaatgttttc accaagctcc ttgctgaccc ggaacttaaa gccgtctctg cctgaataga
     2401 cttctttaag cccttctacg aagttcttgg tttctttttt gtctacttgt acgattactt
     2461 gatattctgt tcccccaata atgtcgtcta atccacgttg tacatagaag gaaaagccgt
     2521 gaccggcgac aagtattgca gctgagagta agacaatttc taagacgatc acgatagcta
     2581 aatcgagttt gattccacca cgcacaaggc tccctccttt cgcttcaatt atagcagaat
     2641 caatggttcc gcctcattgg taagttcctc ttgcggtgcg actttctaac aaaaaaaagg
     2701 gagtctttcg actccccact accaaaccag ctacttacgc ccaagaacac tagaaatgat
     2761 ggatcctgag cttttaactg ccagttttag aaagctttta ttctcggtga aaatgatatc
     2821 aagtgcgtta atcactttgt cgatttcctc tttagttaca gtaagaggcg gctctagcct
     2881 gatgacgttg gggttgttca gcgtgtaggc ggtgataacg ccgtgcttgt tctgaagttc
     2941 tccagcgaca agagcaccaa agtactcttc tgatgctctg gtgagcgctc cgccgctgat
     3001 cttatccagg aagcccttag gctgctcgaa ctcgaggccg atcaaaaggc cttttcctct
     3061 tacctctttg atcaaaccgt gcttatcttt caagcgattc agttcggaaa ggagatagtc
     3121 tccaagttcg agagctcgct gcgggagatt ctcgctgacg atcacctcta gcgcggcgat
     3181 tcctgcagcg gcagaccaag tgttgccgcc aaaagtgctg gtgtggagca ggcacttttc
     3241 tttggaaccg taggcattca tccagatctc ttcagtggtg ataaacaacg atgtgggcat
     3301 tatgccgccg ctgatggatt ttgcaatgca catgatgtcg ggctttgctt catcccactc
     3361 gcaggcaaac atcttaccgg ttctgccgag acctgtttgg atctcgtcgg caattaagag
     3421 cgcgccgtac ttgtggcaga gctcttccgc ggctttaaga tagcctttat cgggaacgat
     3481 gatgccgcct tcaccctgga taggttcaac gatgaagccg gcgacgtctc cttt
//
LOCUS       DAOSDA010000163         3533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16907_length_3533_cov_2.974411, whole genome shotgun sequence.
ACCESSION   DAOSDA010000163 DAOSDA010000000
VERSION     DAOSDA010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16907_length_3533_cov_2.974411"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLW82_04680"
     CDS             <1..555
                     /locus_tag="PLW82_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOJ46602.1"
                     /translation="RERRETWVDAVLYLPRNFRYDLQIHSVNGRIRLNDLDLAAANLE
                     TVNGRVLVERCSGETVNVKTLNGSIDAIGSFKVLDAATQNGSIEYSLLGDSTSASLNS
                     TNGPLRVRLPVSERFDYNIEARTVWGRIFLDLPVRNLEVNRERENYLHATTMAVASYP
                     KDQVVRLQAGTSNGSITILPLENQ"
     gene            569..751
                     /locus_tag="PLW82_04685"
     CDS             569..751
                     /locus_tag="PLW82_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOJ46603.1"
                     /translation="MKKLYRSRQNRIIAGVCGGIAEYLDVDPTVVRLIYLFLGLTSLV
                     VGAVVFYIVAALIIPQ"
     gene            918..1367
                     /locus_tag="PLW82_04690"
     CDS             918..1367
                     /locus_tag="PLW82_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ46604.1"
                     /translation="MFDENRGCLETGELQLRDYGPESFVINIQKAADKNRFFRFALWT
                     GEHLQLTLMEIAVQDSIGLEMHPNLDQFLFITEGSGLVMMGERGDYMNFHQRVCPNDA
                     VFVPAGTWHNLVNTGDEPLKLYSVYGPPAHKHGTIHRTKEEAEEYAQ"
     gene            1609..2265
                     /locus_tag="PLW82_04695"
     CDS             1609..2265
                     /locus_tag="PLW82_04695"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ46605.1"
                     /translation="MRKWLGIVFLVVFVCSAVGFAEAFVEAKFTDKPPKIDGVLDDEI
                     WAKIVPRSDFSISTGGTPDLKTEFWVAWDWDHLYVAIKNYVDTSKLAKNITVDGGATW
                     EDDENSVFLNPAFPEQVSMFQFSFNSIPVRTGLAYGGTPRPEYDFWEVAIVEAEDYYI
                     AEVKIAFDAVWAWPEVGDLWGFNIGRGCTVTGKAYSWSPLKSGNFIDATSFGILEFVK
                     "
     gene            2289..3014
                     /locus_tag="PLW82_04700"
     CDS             2289..3014
                     /locus_tag="PLW82_04700"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOJ46606.1"
                     /translation="MSTSYPLIPRIIAHRGNSSEAPENTLAAVKSALELNVDAIEIDL
                     HLSKDNQLIVMHDDAVDRTTNGTGKIREMTVEELKKFDAGSWKGEAFKGEPIPTFREV
                     LEIVKGRTRLFVEMKVPGAEEVLLQDLRDFDMLDDVVIISFYPEVCARLSELAPDLPC
                     AVLTAVSTFEEFVEVAKRAKTKALDLYHARLDRDAAEKLLDRGYVLWAWTIDDEENMK
                     RAVELGITGITTNKPKLAIELFR"
     gene            complement(3049..>3533)
                     /locus_tag="PLW82_04705"
     CDS             complement(3049..>3533)
                     /locus_tag="PLW82_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46607.1"
                     /translation="VKNLLVVVLFLGLILVNLYVFVSPYLPKRIGLQVYVLPNFFSTE
                     AFDELIASLRKDLGKITVKYSQITEAEIYHNLLLELVKDPERAPDVVMVYDADDLIPG
                     SRERLTKLLPYFHSIEGSRVIRPNPGIPMGFAITRSSKNKDKALKLIDAITDNYYGLF
                     "
BASE COUNT          910 a          803 c          955 g          865 t
ORIGIN      
        1 agagagagaa gagagacttg ggttgatgca gttttgtatc ttcctcgcaa cttcagatac
       61 gatttgcaga tccactcggt aaacggcaga atccgtttaa acgacttgga tcttgccgcg
      121 gccaatctag aaaccgtgaa tggaagagtg ttggttgaaa ggtgcagcgg agagaccgtt
      181 aacgtgaaaa ccttaaacgg cagtatcgat gctattggca gtttcaaggt tttagatgca
      241 gctactcaga atggttccat cgaatactct ttattgggag acagcaccag cgcttctttg
      301 aacagcacca atggaccgct gagggttagg ctgccggtca gcgagagatt cgactacaac
      361 attgaagccc ggaccgtatg gggcaggatt ttcctggatc ttccggtgag aaacctggag
      421 gtaaacaggg agagggagaa ctacctccac gcaactacga tggcagttgc atcctaccct
      481 aaagaccagg tagttcggct tcaagccgga accagcaatg gttccataac cattttgcca
      541 ctggaaaacc agtaaggagt gtgacatcat gaaaaaacta tatcgttctc gccagaatcg
      601 cattatagct ggcgtttgcg gcggtatcgc cgagtatctc gatgtcgacc cgacagttgt
      661 gcgccttata tatctattcc tcggccttac atcgctagtt gtgggagcag tggtgttcta
      721 catcgtcgcc gcacttatta tcccccaata agtcggagaa tcggctattt atacccgcct
      781 gaaagaagca gtctggcggg tcttttctta ttgcttcagg atgaagggct tcacagtagc
      841 ttagagaaat actcctccta tcccaagcga gggccttctc atcctcatat gatgaagcat
      901 gagaagaggt gagagaagtg tttgatgaga atcgagggtg cctggaaaca ggagagttac
      961 agcttcgcga ctacggtccc gagtcatttg tgatcaacat ccaaaaagct gctgacaaaa
     1021 accgcttttt ccgctttgcc ctctggactg gggagcatct gcagctgacg ctgatggaga
     1081 tcgcagtgca ggattcgatc ggtctggaga tgcatccgaa cttggatcag tttctcttta
     1141 tcactgaggg tagtggcttg gtgatgatgg gtgagagagg tgactacatg aactttcacc
     1201 agagagtctg tccgaatgac gctgtcttcg ttcccgccgg cacgtggcac aacctggtaa
     1261 atacgggaga tgaacccctc aagctctatt cggtatatgg accgccggcc cacaagcacg
     1321 gcacaatcca ccgcactaag gaagaagcag aggagtatgc tcagtaaacc tcctcggagc
     1381 tttctgcttt gaaccagtct ggattaatgg cttggcagga ctcccacgct ttgcccacag
     1441 aggtgcatag ttccctgccc ctttttgtca aaaagaggca gggatttttt ctgccacaag
     1501 gaaagttatg gtatgcgaaa acatatgact tgcaagaagt tctgttggtc atttcatcaa
     1561 gatacaagct tcattgctga gtaaactaag gtaaaggggt tggtatctgt gaggaagtgg
     1621 ttgggcatcg tctttttggt tgttttcgta tgcagtgcgg tggggtttgc cgaagccttt
     1681 gtagaggcga agttcaccga caaaccgccg aagatcgatg gggttttgga cgacgagatt
     1741 tgggcgaaga tagtgccgag atcagacttt tccatctcta cagggggaac ccctgacttg
     1801 aagacggagt tttgggtggc ttgggactgg gatcacctgt acgttgccat caagaactac
     1861 gtagatacgt cgaagctcgc caagaacatc accgtcgacg gcggagccac ctgggaagac
     1921 gacgagaact cggtcttcct gaatcctgcg tttcctgagc aggtgagcat gtttcagttt
     1981 tcttttaaca gtatcccggt gcggacgggt ctggcttacg gaggcactcc gagaccagag
     2041 tacgactttt gggaagttgc catcgttgaa gcagaagact actatatcgc cgaggtgaag
     2101 atcgcctttg acgcggtttg ggcttggcct gaagtaggag acctctgggg cttcaacatc
     2161 ggacgcggct gcactgtaac tggaaaggcg tactcctgga gtcctctcaa gtctggcaac
     2221 ttcatcgacg ccacttcatt tgggatactg gagtttgtta aatagacggt gaaaagaggt
     2281 aataaagtat gagtactagc tatcccttga ttccgcgaat cattgcccac aggggcaatt
     2341 cctcagaagc ccccgaaaac actctggctg cggtaaaatc tgcattggaa ctcaatgtcg
     2401 atgccatcga gatcgacctg cacctatcca aagacaatca gcttatcgtc atgcatgatg
     2461 atgccgtcga ccgcactaca aacggtacag ggaaaatcag agagatgact gtggaagaat
     2521 tgaaaaaatt cgatgcaggc agctggaagg gcgaagcgtt caaaggagaa ccgattccca
     2581 cctttagaga ggttctggag attgtaaagg gcagaaccag gttgtttgtg gagatgaagg
     2641 tccccggagc tgaagaggtg ctcctgcagg atctacggga cttcgacatg ctggatgatg
     2701 tggtgattat ttctttttat cccgaggtgt gtgcccgcct aagcgagctt gctccagatc
     2761 tcccctgcgc agtgctcaca gctgtaagca cgttcgaaga gttcgtggaa gtagcaaaga
     2821 gagcaaagac caaagcattg gacctttacc atgcgagact cgaccgcgat gcagctgaaa
     2881 agctcctcga caggggctac gtgctgtggg cttggaccat cgacgacgaa gagaatatga
     2941 aaagagcagt ggagctggga atcacgggta ttacaacaaa caaacctaaa ctggcaatcg
     3001 aactgttcag atagaaaaca acccgccgtt tggcgggttt gcttttagct agaaaagtcc
     3061 gtagtaattg tctgtaatgg catcgatcag tttcagagcc ttgtccttgt ttttggaaga
     3121 tctggtgatg gcaaatccca tggggatgcc ggggttcggt ctgattaccc tgctgccttc
     3181 tatggaatgg aagtacggca ggagtttggt cagtctttct ctggaacctg gaatcagatc
     3241 atctgcgtcg tagaccatta ccacatcagg agcacgttct ggatctttca ccagctcaag
     3301 gaggagattg tggtagatct ccgcttcggt gatctgactg tacttgaccg tgatctttcc
     3361 tagatccttt ctgagagatg cgataagctc atcaaacgct tctgtactga agaagttggg
     3421 caggacgtaa acctgcaggc ctatcctttt gggaaggtac ggcgatacga acacatacaa
     3481 attaactagg attagtccca aaaagagcac cactaccagg agattcttaa ccc
//
LOCUS       DAOSDA010000164         3529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_16932_length_3529_cov_3.048071, whole genome shotgun sequence.
ACCESSION   DAOSDA010000164 DAOSDA010000000
VERSION     DAOSDA010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16932_length_3529_cov_3.048071"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="PLW82_04710"
     CDS             <1..1192
                     /locus_tag="PLW82_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ46608.1"
                     /translation="VRIISSSISNNETERYGGGLFLVNVKETFIVDCTISDNKAASGG
                     GICALDSGEITLINTAICNNTAASAGGIVMTYTGDLPTVASKFFLTDCKISGNTSSHG
                     TLLEHNAAVIVKGSEVHVKSSDLSQNNTRGLTILDANEASVIESVISKNTYGGVLVAS
                     SARIAIHDSTISENDSAYHGAGVCVYNTGSFTMTGSTIAENTTNWHGGGIYVNFEHQF
                     NSKNSFYTVVDISNSDICNNSSAKLGGGIYLSPLKDITITSSRICGNTAEHGGGVYAQ
                     EAGRAGVLTVAGSIVSGNRASADGGGLWIRSLNFTLTGNTITNNTSFYKGGGIYTTSS
                     STITMTDNIISNNSSEQSLGGGLWVGYLVRIKDSQGNILEVPTEVKAINTFFDNTPDD
                     ISFY"
     gene            complement(1331..3226)
                     /gene="uvrC"
                     /locus_tag="PLW82_04715"
     CDS             complement(1331..3226)
                     /gene="uvrC"
                     /locus_tag="PLW82_04715"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOJ46609.1"
                     /translation="MSEDLNIKENAAKTDLLQEKLGLVPDNPGVYLFKNKNGEVIYVG
                     KAKSLRRRVFSYFQSGDKDSPKTRLLVANIADLEYMLVDTEMEAFMLELNLIHKYKPR
                     YNIRLKDDKHYPFIKVTLDEEYPRVFLVRRVKKDGGRYFGPYSDAGAVRNTLNLIHRI
                     FPIRSCKLPVKHGEAVARPCLQYHIGRCLAPCTGKVDLNEYSKLIDRICLFLSNKETE
                     IISELKEEMEKASSELKFERAAVLRDQIRDIEAVTAKQKIISAELDDWDIVALAVGIE
                     YVCAQVYFVRAGRLSGRKNFYLEYNGSSKEEILASFLQQYYGETESIPSLILIPEETG
                     DEVILEEYLRDRKGKKVEVRVPKRGEKKQLLDMAIKNAEQSLEEKELTEDSKYNMTIG
                     ALEDLSEILELSYVPNRLECFDISHIQGTDTVASMTVFAGGEPQKSQYRRFKLRSIGD
                     KPNDYLSMQEVLAWRFTQGLRERELIESGELEKKKAKFAVFPDLIIIDGGKGQLSAAL
                     EILRDLGLGEIPVISLAERLEEIFTKPEGEGILLPNNSRARHLLERIRDEAHRFAITY
                     HRKLRSKRQVKSRLDEIPGIGPKRKKDLLRRFGSIERIKNATVEELLEVDSMNRAAAE
                     RLLDYLK"
BASE COUNT          889 a          890 c          766 g          984 t
ORIGIN      
        1 agtccgcatc atcagtagct ctatcagcaa taacgagact gaacgttatg gtggtggact
       61 ttttctcgtt aatgtgaaag aaaccttcat agtagactgc actataagtg ataacaaagc
      121 tgcctctggc ggaggcattt gtgctttgga ttctggagaa atcaccttga tcaacactgc
      181 aatttgcaat aacaccgcag caagtgctgg tggtatcgta atgacgtata ctggagatct
      241 cccgacggta gccagcaagt ttttcctaac cgactgtaag atatccggta atacgagttc
      301 tcacggaacg ctacttgaac acaatgctgc tgttatagta aagggcagtg aggtccacgt
      361 aaaaagcagc gatctgagtc aaaacaacac tagaggatta accattttgg acgctaatga
      421 agccagcgtt atcgagagtg taatatccaa aaacacatat ggaggagtct tagttgcctc
      481 tagcgcccgc atcgcaatac atgacagtac aataagtgag aacgacagcg cttaccatgg
      541 tgccggggtc tgcgtttaca acacaggtag tttcaccatg acaggcagca ccattgccga
      601 gaacacaaca aattggcatg gtggcggaat ctatgtaaac ttcgaacacc aattcaattc
      661 aaaaaactcg ttctacactg tggttgacat atccaactcg gatatctgta acaactcttc
      721 agcgaaactt ggtggcggga tctatctttc acctcttaaa gatatcacca taacaagcag
      781 ccgtatctgt ggcaacactg ctgagcatgg aggaggagtt tacgctcaag aagcaggcag
      841 agccggtgtt ttaaccgtag caggtagcat tgtcagcggc aatagagcct cagccgatgg
      901 cggtggctta tggatccggt ctttgaactt caccttaaca gggaatacca taactaacaa
      961 tacttctttc tacaaaggtg gggggatcta taccacgagt tcctcaacga taaccatgac
     1021 cgataatata atcagcaata actcatctga gcagagcctc ggtggcggtc tctgggttgg
     1081 ttacttggtt aggatcaaag acagccaagg caacatctta gaggtcccaa cggaagtaaa
     1141 ggccatcaat acgtttttcg acaacactcc agatgacatc agcttttatt aaccctaacc
     1201 gaagcaactc ctctcaggtt gagttccaaa aaagagaacc tcaatgcttg caagcgttga
     1261 ggttctttgc atatcttctc ttaagagagt acactgcaca cgattcttca acaagaccgc
     1321 atgtttggcg ctacttcaag tagtcaagga gtctctctgc agcagccctg ttcatcgagt
     1381 caacttccag aagttcctcc actgtagcat tctttatacg ctcaatcgaa ccgaacctgc
     1441 gcagcagatc cttctttctt ttgggaccaa taccggggat ttcatcaagg cgagacttaa
     1501 cctgcctctt tgatcgcagt ttacggtgat aggttatggc aaacctgtgg gcttcatctc
     1561 gaatacgctc aaggaggtgc cttgctcttg agttgttagg aagaagaatg ccttcccctt
     1621 caggtttcgt gaagatctct tcaagccgct cagcaagaga gatcaccggg atttcgccta
     1681 accctaagtc tctcaagatc tccaatgcag cagaaagctg ccccttccca ccgtcgataa
     1741 tgattaggtc tgggaaaacg gcgaacttgg cctttttttt ctccagctca cctgactcta
     1801 ttaactccct ctctctaagt ccttgagtaa accgccaggc taggacttcc tgcatagaaa
     1861 gatagtcgtt gggtttatct ccaattgaac gcagtttaaa ccttctatac tgcgacttct
     1921 gcggctctcc accggcaaaa accgtcatgg aagctacagt atccgtccct tgaatgtggg
     1981 agatatcgaa gcactctaag cggtttggta catacgaaag ctcgaggatt tccgataggt
     2041 cttctaaagc tccgatggtc atgttgtact tgctgtcttc tgtgagctcc ttctcctcca
     2101 aactctgctc agcgttcttg attgccatgt ctagaagctg tttcttctct cctcttttgg
     2161 gaacccgcac ttccactttt ttgcccttac gatctctgag atactcttcc agtataactt
     2221 catcgcctgt ttcttcaggg atgagaatca ggctgggaat gctttctgtc tcgccgtagt
     2281 actgctgaag aaaggaagca agaatctcct ccttgcttga accgttgtac tctaagtaga
     2341 agttttttct gcctgaaagc ctgccagctc tcacaaaata gacctgggca cagacgtact
     2401 caatgcccac ggcaagtgca acgatatccc aatcgtcgag ctctgcagag atgatcttct
     2461 gcttggcggt cacagcttca atatcgcgaa tctgatctct aagtactgct gccctttcga
     2521 acttcagctc gctggaggct ttttccatct cttctttcag ttcgctgata atctccgttt
     2581 ctttgttgct cagaaagagg cagatcctat cgattaactt gctgtactca tttaagtcta
     2641 cctttcctgt gcacggtgca aggcaccgtc cgatatgata ctgaaggcag ggtcttgcca
     2701 ctgcttctcc atgctttacc ggcagtttgc agcttctgat gggaaagatt ctgtgaatga
     2761 ggttcagtgt gtttctcaca gctcctgcat cagagtaagg accgaaatac ctgcctccat
     2821 cttttttcac tcgcctgacc agaaacaccc tggggtactc ctcgtccagg gtgaccttga
     2881 tgaacggata atgcttgtcg tctttgagtc tgatgttgta cctcggtttg tatttgtgaa
     2941 tgaggttcaa ctccagcatg aaggcctcca tctcggtgtc taccaacatg tactctaagt
     3001 ctgcgatgtt ggccacaaga agccgggttt tcggagagtc tttgtctccg gactggaagt
     3061 acgaaaagac tctacgcctg aggctcttgg ccttgccgac atagatgact tcgccgtttt
     3121 tatttttaaa aagatagacc ccaggattgt ctgggacaag ccccagcttc tcctgaagca
     3181 ggtccgtctt tgctgcattt tctttgatgt ttagatcttc agacattaaa ctaccctccg
     3241 aactcacgta gcctgataag ccttcactct ctacttcctt ggtaaaactg ctttacttcc
     3301 atggatcctc gcgattttta aggactacca ttaacatacc cgccaaggca gaagcaaaca
     3361 cctaaggttt ggccttccag ttgacgagga atgcatcaac ttcggtgctt ctgttttaaa
     3421 cagtactctg catcacttaa cttccctttg gcaaactcga tcattcgctc tgcttcaact
     3481 atgcgccctt ctgcgtctgt aagctttgta gcgccgttta ccggcttcg
//
LOCUS       DAOSDA010000165         3504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_17112_length_3504_cov_3.828646, whole genome shotgun sequence.
ACCESSION   DAOSDA010000165 DAOSDA010000000
VERSION     DAOSDA010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17112_length_3504_cov_3.828646"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1427
                     /locus_tag="PLW82_04720"
     CDS             90..1427
                     /locus_tag="PLW82_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46610.1"
                     /translation="MLKQPSGKYTGIVTMSDGETPVPADVYVGGEKYKTVGEDGKFEI
                     TLKPGTYKIYAEYMGVKSEEVTLNATSQGGSVVVKIDGLDKVSGTLKAADGTALANQA
                     FKISNVDVTTDANGKYDVVLTYNKHTVLVNYKGYEYSKEQTFQAGTNDIVVDDVKQLT
                     LTLKHKDGSALSGAKATVKVGGFTHQVTADAEGKITFLGVAKPGTLTADISGDLKMPV
                     AEEFSKFQNTVNVDFATATEAEFDVADPDFVDTFDDGLDNWIEGEDWILNDWQETIKG
                     AVVQNGVLTRSEYVPHKTSGVKLKDVEITDAAVVIKVRLLSEREGQNAGMRVHLRNDS
                     DKFNHGYGLNFTATSCLMENWLGNLTSKGAAVKVDPGKGQFPGDDIWCTVTLTLVDHT
                     MRVYVDGNPVLVNPEKGPLVFVEENEEWQHDSGYLWIEYSGALEIDEVRVYHL"
     gene            1804..2826
                     /locus_tag="PLW82_04725"
     CDS             1804..2826
                     /locus_tag="PLW82_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ46611.1"
                     /translation="MGNLRSAIIGCGGVARVHAHAINDNTDAQLVATVDIKEERAIRY
                     AEKYGVEGCKPYTDYKELLKDPTIDVVHICTPHHLHAPMVIDAVAAGKHVIVEKPVAI
                     NVGQAKAMIEAVKKSDRVVGVILQNRYNLASCEAKKVIDEGKLGKILGARAFVTWYRK
                     EEYYKDSDWKGTWDMEGGGVLIDQAIHTIDLMQWLVGEIDWVKGNIANHFHPYIEVED
                     VAEGLIQFKNGARGIIYATNFYSYDADIILEIHGEKGKVFIEKDRARIEVDDMVYTVE
                     ESKEVKEGKSYWGSSHATQIADFYTDIKQGKRHWIGPEEAKVPLDIVLAIYRSKGQAI
                     QLPLTE"
     gene            2929..3495
                     /locus_tag="PLW82_04730"
     CDS             2929..3495
                     /locus_tag="PLW82_04730"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOJ46612.1"
                     /translation="MEKQFWQDYRSSFFAVPVVVVSVDGKTRPNLITLGWTGIVNSDP
                     LMVGISVRPSRYSRDLLHEHKVFGLNIPNKALAAGTDYCGIKSGRDVDKVSELGWHVF
                     RGRSLGVPLVEEFPVNAECQITQIIPLGSHELFLAEVKGVYIAKEYLKGNRVDWTTAE
                     GLVYGSQTYYVVKEGVFGQGDSYRELKK"
BASE COUNT         1024 a          736 c          922 g          822 t
ORIGIN      
        1 aactaggagg gtgtttgatg aggttcaaaa gtaagttact cgtttcattg gtactcgtcg
       61 tcgctgttgt gatgcttagt ggatgtaact tgttaaaaca gccaagtggc aagtacaccg
      121 gtattgtcac catgagcgat ggtgaaacac cagtgccagc cgatgtttat gtcggtggcg
      181 agaagtacaa gactgttggc gaagatggta agttcgagat tacactcaag ccaggcacct
      241 acaagattta cgcagagtac atgggagtaa agtctgaaga agttacactc aatgcaactt
      301 ctcaaggcgg atcggtagtg gtcaagatcg atggtcttga caaagtcagc ggtactctca
      361 aggcagcaga tggtactgcg ctagccaacc aagctttcaa aatcagcaat gtagatgtta
      421 caaccgatgc taacgggaag tatgacgtgg tgcttacata caacaagcac acagtgttgg
      481 tcaactacaa gggttatgaa tactctaagg agcaaacttt ccaagcaggt acgaacgaca
      541 tcgtcgttga tgacgtaaaa cagttgacat tgactctgaa acacaaagac ggttctgctc
      601 tttccggagc aaaggccact gttaaagtgg gaggtttcac tcaccaggta acagcagatg
      661 ccgaaggtaa gatcacattt ctaggcgttg caaaacccgg aacacttaca gctgacattt
      721 caggcgatct gaaaatgccc gttgctgaag agttttccaa gttccaaaat accgttaacg
      781 tggattttgc cacagcaaca gaagccgagt tcgacgttgc agatccagac tttgtggaca
      841 catttgacga cggactggat aactggatcg aaggcgaaga ctggatcttg aacgattggc
      901 aggagaccat caagggcgct gtagttcaaa atggtgttct cacaagaagt gagtatgtac
      961 ctcacaaaac aagcggcgta aaactgaagg atgtagagat cacagacgca gctgttgtca
     1021 ttaaagtccg gctcttaagc gaaagagagg gacagaatgc cggtatgcgc gtacacctaa
     1081 gaaacgactc ggacaagttc aaccatggtt acggcttgaa cttcaccgca accagctgtt
     1141 taatggagaa ctggctgggt aacctcactt ctaaaggtgc agctgtgaaa gtagatcctg
     1201 gaaaagggca gttcccagga gatgacatct ggtgtacagt gacgctgact ttggtagatc
     1261 acactatgag agtctacgtc gatggcaacc cagtgcttgt caatcccgaa aaaggtccgc
     1321 tcgtattcgt tgaagaaaac gaagagtggc aacacgactc cggttatctc tggatcgagt
     1381 actccggagc actggaaatt gacgaagtta gagtatatca cctataatct gcaaaatcgt
     1441 agctagggca gaaactgccc tagctttttt gttgcaaaaa cgggtgtaag ctttactggc
     1501 aggagtcttt tgtggacttg gatcttcaat ctgctaaaat aaccaagctt taaatgcaga
     1561 aaaatgatag aatataaagg atggtttaaa ggccacgcgc aaccggggct ttcggttgtg
     1621 ctttgctttt tgactgattt ctttttcagt tcaaagtact gatgtttact gctgggcttg
     1681 cagggttaag agagaattta gagaagtaac ctcacttagt tagctttacg gtttgaaaag
     1741 gttaagaatg atatttatga taaaatgtat atggttcaaa tgtaggaagg agttaatgta
     1801 cctatgggca atttacgatc agcaataatc ggctgtggtg gcgttgcaag ggtgcatgcg
     1861 catgccatca atgacaacac agatgcacag ctggttgcaa cagtggatat caaagaagaa
     1921 agagcaataa ggtatgcgga aaagtacggt gtagaaggtt gcaaacctta cacggactac
     1981 aaagaactgc tgaaagatcc aacgattgat gtggtccaca tatgtacacc gcaccacctg
     2041 cacgcaccga tggttatcga tgcagttgct gcaggtaagc acgttatcgt tgaaaaacct
     2101 gtagcaatca acgtcggaca ggcgaaagcc atgatcgaag cggtcaagaa aagcgacaga
     2161 gttgttggcg taattctgca gaatcgctac aaccttgcca gctgtgaagc taagaaggtt
     2221 atcgatgaag gcaaactggg caagatcctc ggtgccagag cttttgtcac atggtaccgc
     2281 aaagaggagt actataaaga tagcgactgg aaaggcacat gggacatgga aggtggagga
     2341 gtcctcatcg accaggcaat ccacaccatc gatctgatgc agtggctggt aggcgagatt
     2401 gactgggtga agggcaacat cgccaaccac ttccacccat acatcgaagt cgaagacgtt
     2461 gcagaaggcc tcatccagtt caaaaacggc gctcggggca tcatctatgc caccaacttc
     2521 tacagctacg acgccgatat aattctggag atccacggag aaaagggcaa agtctttatc
     2581 gagaaggaca gagccagaat cgaagtggac gacatggtct atacagtaga ggagtccaag
     2641 gaagtcaaag aaggcaagtc ctactggggc tctagccacg ctacgcagat cgccgacttc
     2701 tacaccgaca tcaagcaggg caaacgccat tggattggtc ctgaagaggc aaaagtgccc
     2761 cttgatatcg ttcttgccat ctacaggtcc aaaggacaag cgatccaatt accccttacg
     2821 gaataattct gatttttagg ttgatgcgga aaggttttag cctatattac acaagctgcc
     2881 aaagggcagc ttgttccatc taaaacagca tcgaagggga tgagtctaat ggaaaaacag
     2941 ttttggcagg attacaggtc ttcgtttttc gccgttccgg tagtggtagt ttcggtggac
     3001 ggaaagacaa ggcccaacct cataacgttg ggctggaccg ggatcgtgaa ctcagatccg
     3061 ctgatggtgg gcatttcggt gcgccctagc cgctactcca gggaccttct ccacgagcac
     3121 aaggttttcg gcctgaacat cccaaataag gcacttgcag ctggtaccga ctactgtgga
     3181 atcaagtcgg ggagggatgt agataaggtc tcggaactcg gttggcacgt cttccgcggg
     3241 cgtagtcttg gcgttccctt ggttgaagag tttccggtga acgccgaatg ccagatcacc
     3301 cagatcattc ccctgggcag ccacgagctt tttttggcag aggtaaaagg ggtctatatt
     3361 gccaaagagt atttaaaggg taacagggtt gactggacca cagctgaagg cctggtttac
     3421 gggtctcaga cttattatgt agttaaagaa ggagttttcg gtcaaggaga ttcctacagg
     3481 gagctaaaga aatgacgttt ttag
//
LOCUS       DAOSDA010000166         3503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_17118_length_3503_cov_2.764501, whole genome shotgun sequence.
ACCESSION   DAOSDA010000166 DAOSDA010000000
VERSION     DAOSDA010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17118_length_3503_cov_2.764501"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /gene="hslU"
                     /locus_tag="PLW82_04735"
     CDS             complement(<1..1337)
                     /gene="hslU"
                     /locus_tag="PLW82_04735"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOJ46613.1"
                     /translation="MEGLTPKEIVEYLDKYIVGQHKAKRAVAVALRNRHRRRMLPPEM
                     REEVAPNNILMIGPTGVGKTEIARRLAKLVGAPFVKVEATKFTEVGYVGRDVESIIRD
                     LVEEARRLVRSRKIEEVREIARAQAEINLLDLLAPTRKEEKSNPFSILFGSEQADQRP
                     QVSVEEKEEIRRKLEAGELEDELIEITVPEREAQLDLFSFGIDDSGVNLQDVFGGFLP
                     KRTVKKKVTVKKAREILTELEAEKLLDKDSINQEAIELAENEGIVFIDEIDKIAGSSS
                     NSGPDVSREGVQRDILPILEGSVVATRYGHVKTDHVLFIAAGAFHVSKPSQLIPELQG
                     RLPVRVELSSLTEEDFVRILEEPKNSLLQQYKELLKTEGLEVEFTKSGIREMAACARK
                     VNENTEDIGARRLNTILETVLEEVAFDAPELSGQKVVIDDKYVEMKLKDLIQDR"
     gene            complement(1337..1876)
                     /gene="hslV"
                     /locus_tag="PLW82_04740"
     CDS             complement(1337..1876)
                     /gene="hslV"
                     /locus_tag="PLW82_04740"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOJ46614.1"
                     /translation="MFKGTTIIAVRRNGQVAIGGDGQITFGENTVFKTTANKLRRLYS
                     GKVIAGFAGSVADAFTLFERFEKKLSEHRGNLVRASVELAKEWRSDRILQKLEALLIV
                     ADSEHIFVISGSGEVIEPDDNIAAIGSGSSYALSSARALYRHTNLSARTIVEESLKIA
                     AEICVYTNDHISVLELEGE"
     gene            complement(1869..2789)
                     /gene="xerC"
                     /locus_tag="PLW82_04745"
     CDS             complement(1869..2789)
                     /gene="xerC"
                     /locus_tag="PLW82_04745"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOJ46615.1"
                     /translation="MLVLKKRVEDWFDRFMFFLQAEKGYSEHTCQAYALDIREFLDYV
                     EAEDSYELTTAKVRQYLGYLYDLKRERTTVARKLAALRSFFRYLEKRNLFQDNPIVDI
                     KTPKKGQYLPLFLYPKDIEKLLETPDLTTPIGRRDRAILELFYSAGLRLSELVGLNLT
                     DIDWDIGYLRIRGKGGKERITPLGYYAYNYLEDYLTLARDALLKGKETEAVFVSSRGM
                     RINPRTVEYMVDKYVKIAGLNSGISPHSLRHTFATHMLENGADIRSVQELLGHESIST
                     TQIYTHLSRSRLREVYFKAHPRAKGESEDV"
     gene            complement(2789..>3503)
                     /locus_tag="PLW82_04750"
     CDS             complement(2789..>3503)
                     /locus_tag="PLW82_04750"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOJ46616.1"
                     /translation="PARYTQASLVKELEELGIGRPSTYATIIDTIKKRGYVVEEEKKL
                     VPTELGQVTVDLLKHYFDDIVDVDFTRELEARLDAIEEGENSPVKILKGFYTAFDEKL
                     KKAEKTADKVEIADEQTDEPCPNCGRLLVVKMGRYGRFLACPGFPECRFTKPLLKEMP
                     GSCPLDGGKLVERRSKKGRVFYGCENYPDCEFTTWYTPTAKNCPKCGKTMAVKGRGAK
                     REYLCLSSECGNRMPAGDE"
BASE COUNT          775 a          964 c          761 g         1003 t
ORIGIN      
        1 cgatcttgaa tgagatcttt cagcttcatt tccacatatt tgtcgtcgat taccactttt
       61 tggccagaaa gctccggtgc gtcaaaggcc acttcttcca gaacggtttc taaaatggta
      121 ttgagccttc gcgctccgat gtcttcggtg ttctcgttga ctttgcgggc acatgcagcc
      181 atctctctaa tgcccgactt ggtgaactct acctccaaac cctcggtttt caggagttct
      241 ttgtactgtt gcagaagcga gttcttgggc tcttccaaga ttctgacaaa gtcctcttcg
      301 gtgagactcg atagctccac cctcactggg agacgacctt ggagttcggg tatcagttga
      361 gaaggcttgg aaacatggaa agctccagct gcgatgaaga gaacgtggtc ggtcttcaca
      421 tgaccgtacc ttgtggctac taccgatcct tcgaggatgg gcaagatgtc tctctggact
      481 ccttctctgg aaacgtccgg tccgctgttg gaagaacttc cggctatttt atcaatttca
      541 tcaatgaaga cgataccttc gttttccgca agttcaatag cttcctgatt gatggaatcc
      601 ttatcgagaa gtttttccgc ttccagttca gtgaggattt ctctcgcttt ctttacggta
      661 accttcttct tcaccgttcg tttgggcaaa aagccaccaa atacatcctg gagattgaca
      721 cctgaatcat caataccaaa ggaaaaaaga tccagctgag cttctctctc tggcacagtg
      781 atctcgatta gctcgtcttc cagctcgccg gcttcaagct tcctcctgat ctcttctttc
      841 tcttcaacgg aaacctgcgg tcgttgatca gcctgttcgg agccaaagag gatggaaaag
      901 gggttgctct tctcctcttt cctcgtggga gcgagaaggt ctaagaggtt gatctctgcc
      961 tgcgcccgcg caatttctct gacttcttca atcttcctac tgcggacaag cctcctggct
     1021 tcctcaacca aatctcggat gatcgattcc acatctcttc ccacatagcc cacttcggta
     1081 aatttggttg cttcaacctt gacaaaaggt gcaccaacca gcttggcaag tcgtctggca
     1141 atttcagttt tgccaacgcc tgtaggtccg atcatcagaa tgttattagg ggctacctct
     1201 tccctcattt ccggcggaag catcctgcgt ctgtgcctgt ttctcagcgc aacagccaca
     1261 gcccttttgg ccttatgctg gccaactata tatttatcga gatactcgac tatctctttt
     1321 ggggttaaac cctccatcac tcaccctcca actctagaac agaaatatgg tcgttggtgt
     1381 agacgcatat ctctgcagca atcttcaagc tttcttccac aatggttctg gcgctgagat
     1441 tggtgtgccg gtacaacgct ctggcactgc tcaacgcata gctgcttccc gagccgatgg
     1501 cggcaatgtt atcgtctggt tcgataacct ctccagagcc agagatgaca aagatatgct
     1561 cactatcggc aacaatgaga agagcttcta gcttctggag aatcctgtcg ctccgccatt
     1621 ctttagccaa ctcgacgctg gctctcacaa ggtttcccct gtgctcagaa agcttcttct
     1681 caaagcgctc aaacagcgta aacgcgtctg ccaccgaccc ggcaaaaccg gcaataacct
     1741 ttccggagta tagcctccgc agcttgttgg cggtggtttt gaacacggta ttttcgccaa
     1801 aagtaatctg cccgtcgcct ccaatggcta cttgcccgtt gcgacggact gcaatgatgg
     1861 tagttccttt aaacatcttc actttctcct ttagccctgg gatgagcctt aaagtaaacc
     1921 tcgcgcagac ggctccggct caagtgagtg tagatctgcg tggtggaaat gctctcgtgt
     1981 cccaaaagct cctgcacaga ccggatatcc gcgccatttt ccaacatatg ggtggcaaaa
     2041 gtatgtctga gggagtgcgg cgagatgccg ctgttcaacc ctgcgatctt cacatactta
     2101 tctaccatat attcaactgt acggggattg atgcgcatcc cccggctgct cacaaacacc
     2161 gcttctgtct ctttaccttt cagcagtgca tcccttgcca gggtaaggta gtcctcaaga
     2221 tagttgtagg cgtagtagcc cagaggagta atgcgttcct ttcctccctt gcccctaatc
     2281 ctgaggtagc cgatatccca gtcgatatct gtaaggttca ggccaacaag ctcgctgagt
     2341 ctaagacctg cgctgtagaa aagctccagg atcgctctgt cccgtctccc tataggcgtg
     2401 gtgaggtctg gggtttctaa aagcttctca atgtcctttg gatagagaaa caaaggcaga
     2461 tactgccctt ttttcggcgt tttgatgtca actatcggat tatcttgaaa tagattcctt
     2521 ttttccagat agcgaaaaaa actccttagg gctgcaagct tcctggccac tgtggttctt
     2581 tcccttttta aatcgtacag ataaccgaga tactgcctga cctttgccgt ggtaagttcg
     2641 taactgtctt ctgcttccac ataatccaga aactcccgga tgtccagagc atacgcctga
     2701 cacgtatgct ccgagtagcc tttttctgcc tgcagaaaga acatgaagcg atcgaaccag
     2761 tcttctacac gttttttaag aaccagcatc actcatcacc agccggcatt ctgtttccgc
     2821 actcgctgct cagacacaga tactctcttt ttgctcctct ccccttcact gccatggtct
     2881 tgccgcattt cgggcagttc ttcgctgtag gagtatacca ggtggtaaac tcgcagtcgg
     2941 gatagttttc acatccgtaa aataccctgc cttttttcga cctgcgctca accaacttac
     3001 ctccatcaag tgggcaggag ccaggcatct ctttaaggag cggtttggta aacctgcatt
     3061 cgggaaaacc gggacacgcc aaaaacctcc cgtaacggcc catcttcact actaacagtc
     3121 tcccgcagtt aggacagggt tcatcagttt gttcatctgc gatttccacc ttgtccgcag
     3181 tcttttccgc ctttttcagt ttttcatcga aagccgtgta aaaacccttt aaaatcttta
     3241 ccggcgagtt ttctccttct tctatggcat ccaaccgtgc ttctagttcc ctagtgaaat
     3301 ccacatcgac aatgtcgtcg aaatagtgtt ttagaagatc gacagttacc tgtcccagct
     3361 cggttggcac gagttttttc tcctcttcaa cgacgtatcc tctttttttg atggtatcga
     3421 taatcgtcgc ataagttgaa ggtctgccga tgccaagttc ctctagttcc ttgacgaggc
     3481 tggcctgagt gtatcttgcc ggt
//
LOCUS       DAOSDA010000167         3469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_17309_length_3469_cov_3.240773, whole genome shotgun sequence.
ACCESSION   DAOSDA010000167 DAOSDA010000000
VERSION     DAOSDA010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17309_length_3469_cov_3.240773"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..136
                     /locus_tag="PLW82_04755"
                     /pseudo
     CDS             1..136
                     /locus_tag="PLW82_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
     gene            551..1327
                     /locus_tag="PLW82_04760"
     CDS             551..1327
                     /locus_tag="PLW82_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46617.1"
                     /translation="MQESLVVLECLRRSSEKRILTRVYRQLYNPDFYKPEYSREIVDA
                     LKAERYRMLESEHLSELKSAVAAVLGAVYQVEFKTDKILGFLKEVDLKAEELSDFPLE
                     IKEKIDDGRFWHLVFYLKPWKAYFPTEKVLIWDEGIYRFSPDGDVKSFLLEGLGLRWQ
                     KGRITLDPSFKAKLLRPYMREGKPIHRGSLLNLPMERIISFYKEEEKRFKRLLSLLSK
                     PSEEADMLYILQRSLEKTLACKYRTSTKEIRKRLKLIQAD"
     gene            1389..3413
                     /locus_tag="PLW82_04765"
                     /pseudo
     CDS             1389..3413
                     /locus_tag="PLW82_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
BASE COUNT          977 a          807 c          919 g          766 t
ORIGIN      
        1 gttttatcgc gtagcgtaca acatcgctga agcggacaag ctatacgata agaaagccaa
       61 catcaaaaag aaagcagaac gcttctacga gctgatgacc agtctcaaat tcatgccgaa
      121 tagtcccact ctgatggtgc gccaagacag ttagcaaata ctgccgaaaa cggtacagga
      181 ctcgggaaac gtcctgttag tgaacctgct aaccgcaagg gacaacagca tgcaggtcgc
      241 taagcatcac atggctaaag ctggattgct tgaactctag ttcttctgga tcaagtgggg
      301 atctgcgtaa gcatctggtt ttaaagaaag atgttcatcg gtctttcttt attcccgaaa
      361 ccagacaggg caccagacag gtgctgaaaa gaacgaacgg agagcctaaa tgatgcgttg
      421 tgtttgctaa tgaacttggg ataggtggca gagccactta cggagctctc gtagtagtca
      481 aggcggtaac gccgtcctaa tggcgaaggg gagcaggtca gccaggtact gagaaacgga
      541 ggtatgcgta atgcaggaaa gcctcgttgt cttggaatgc ctgcgaagga gttctgagaa
      601 gaggattctt acacgggtat acaggcagct ctacaatccg gatttctaca aaccggagta
      661 cagcagggaa atcgttgatg cgctgaaggc tgagaggtac agaatgctag aaagtgagca
      721 tctatccgag ctgaaaagcg cagtggccgc ggttttaggt gcagtctacc aggttgagtt
      781 taagaccgac aaaatcctgg gctttctgaa ggaagttgac ttgaaagctg aagagctttc
      841 cgactttcct ttggagatca aggagaaaat cgacgatggt agattctggc acttggtttt
      901 ttaccttaaa ccctggaaag cgtatttccc tacagaaaaa gtacttatat gggatgaggg
      961 catctacaga ttcagcccag acggagacgt taagtctttt ttattagaag gactcggtct
     1021 cagatggcaa aaaggcagaa ttactctgga tccttctttt aaggccaaac tcctcaggcc
     1081 ttatatgcgg gaaggcaagc ccatccacag aggcagccta cttaaccttc caatggaaag
     1141 aatcatcagc ttctacaaag aagaagagaa gaggttcaaa cgcctgcttt cactgctttc
     1201 caaacccagt gaagaagcag atatgctgta cattttgcaa agaagcctgg agaagacttt
     1261 ggcgtgtaag tataggacct cgaccaagga gataagaaag aggctcaaac tcattcaagc
     1321 tgactagaga gccgtatgcg gggaaactcg cacgtacggt tcggaggggg ggatactccc
     1381 taccctacaa tgcaggaaga gaactacaac aactttcggc atgttttgta cttcccattg
     1441 aggattcaat ggagagcata tttgaaagtg tcaagaacgc agctctcatc cacaaaagcg
     1501 gtggaggtac aggtttttcg ttttcccgga ttcgtccagc caacgacagt gtcaagacaa
     1561 ctggcggcgt ggcttccgga cccatatcgt tcatgaaggt tttcaacgcg agcacagaag
     1621 tgatcaagca gggtggcgtg agaagaggtg ccaacatggg catcctcaga gtcgatcatc
     1681 ctgatattct agagttcatc acctgcaaac gcgataacac tgaactaacc aatttcaata
     1741 tatccgtcgg aatcaccgaa gcatttatgc aagcagtaga acatgataca gagtacgacc
     1801 tgctcaaccc gcgcaacgga aaggtcgtgc agaagctgag ggccagagag gtgttcgacc
     1861 tcatggtgga gatggcctgg ctcaatggag agcccgggat cgtctttttg gatagaatca
     1921 atcgcgacaa tcccactcca aaacttggcg agatcgaatc caccaacccc tgcggtgaac
     1981 agccgctttt gccctacgag gcttgcaacc tcggttcaat taacctctcc aagttcgtca
     2041 aagacgggaa gatcgattgg gaagacttgg ggcaggttgt cgatttggca gtggacttcc
     2101 tcgacaacgt tatcgatgtc aatcgctatc cgctccccga gattgaagag ctggccaaag
     2161 ccaacagaaa gatcggtctc ggcgtaatgg gctttgcaga tatgctgttc atgttggaga
     2221 tcccgtacaa ctcacaggaa gctctggatc tcggagccca gatcatggcc ttcatcgaca
     2281 agcgctcaaa ggaaagatcc caggcaatcg ccttcgagag gggcagcttc cccaacttcg
     2341 aaaagagcat ttacaaggac aagtatcagg ctctaagaaa tgccaccact acgacgatcg
     2401 cacctacagg atcgatcagc atcattgcag ggtgctctag cggcattgaa ccgttgtttg
     2461 cactgtcctt tgttcggacg attttggatg agcagaggct tctggaggtt catccgatct
     2521 ttagagaagt tgccatgcgc gaagacttct attcagatga gctgatggag aaaatcgctg
     2581 aagaaggttc catccagcat atggaagaga ttcccgagca ctggagaaga gttttcgtga
     2641 cagctcacga tattacgcct gaatggcacg tacgcatgca agcagtcttc cagcaaaacg
     2701 gtgtggataa tgccgtaagt aagaccgtta acttcccgaa agaggcaacc agggaagata
     2761 ttgctaaggt gtttagactc gccttcgagc tgggctgcaa aggcgtaacc gtctatcgcg
     2821 acggttccag acagatgcag gttctaagca ctgggaaaac cgaatccaag acccaggaag
     2881 aggacaccca caggatcagt cctagggtac ggccaagcgt aacgtacggc aggactgaaa
     2941 agatcaaaac cgggtgcggc agtttgtatg taaccatcaa cgaagacgaa aacggcctct
     3001 gcgaattatt cgccaggctg ggtaaatccg gaggttgtgc atcaagccag atggatgcca
     3061 tcagcaggct gatctccctg gctatgaggg ccggagtatc taccgaagcc atcaccaagc
     3121 acctgagggg cattcgctgc ccatcacctg cctggacaga aaacggagta gttctctcct
     3181 gcccagatgc catcggcctt gccatcgagc actatgttgc ctggcgccaa aacggcaatg
     3241 gcgaacacgc cagcgagatg gtgtcggaaa aactgccgat ggccgatgta gtgaagcaga
     3301 cctccactgt agctgaaagc tccatgggag cttgccctga gtgcggtggg catatgaagc
     3361 atgaagacgg ctgcatgacc tgtcttttct gcggattttc aagatgcggt tgatatttag
     3421 caggatttgt gcccaaatgc ggtaatactg taactgtaga atcgttatt
//
LOCUS       DAOSDA010000168         3452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_17425_length_3452_cov_3.058581, whole genome shotgun sequence.
ACCESSION   DAOSDA010000168 DAOSDA010000000
VERSION     DAOSDA010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17425_length_3452_cov_3.058581"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLW82_04770"
     CDS             <1..204
                     /locus_tag="PLW82_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46618.1"
                     /translation="GGGGANIHSFTEAKKHRVRLAALNIGEGEKPLEEAEKAYAQIKT
                     DIVSEKSAVFYREKAGVGVRSRS"
     gene            243..1091
                     /locus_tag="PLW82_04775"
     CDS             243..1091
                     /locus_tag="PLW82_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ46619.1"
                     /translation="METFVARVNEQKKQLKRPLVGLALGSGAARGMAHIGVLQVLFEE
                     DIPIDCITGTSIGSLVGAMILSGWTPQFLGRFARAVGKEHMVSVLDFVIPPKTGLING
                     NRIKVLLERVLKIKNIEELPKPFAAVSTDIATGERVVHDRGSLLEGIRASISVPGVFV
                     PAEERESGRLLVDGGVVDPVPLDVLVEAGVEIQIGVNILRKPDTAEKPKDVIDILLNS
                     IDIMGAQIFELKSTGKAIVLEPLLGHRFGSMDFHKAEPMIELGRKCALEKLPEIKERL
                     RSEGVL"
     gene            1092..1649
                     /locus_tag="PLW82_04780"
     CDS             1092..1649
                     /locus_tag="PLW82_04780"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOJ46620.1"
                     /translation="MLSVEEVAGAAAGCVRCSIRKNTPEVVFGKGSPDARIMVVLEAP
                     DADAAKEGKPISGEAGELLYNIFAASHIKGGDVYVTNLVKCNPPEERTPTEEEIGSCK
                     TWLDLEIAAVQPDYLILMGVAPLRAFFGEDAAIQEFRGKWHSFGEIKVLCTYQPKTLL
                     KKPEYKRMVWEDFKMLMRDLGILKP"
     gene            1749..>3452
                     /locus_tag="PLW82_04785"
     CDS             1749..>3452
                     /locus_tag="PLW82_04785"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ46621.1"
                     /translation="MINLVILPKGERVLKKYDTSQIRNVAIIGHGGTGKTMLTEAILF
                     HAGATKRKGSISDGNTVSDYDPEEIKRGTSISTALTTCQWKDTKINLLDTPGFFDFVG
                     EVVAALEVTEGAVSVLSATDSLPVGFFTTWKLADERKLARTIFINKMDKENASFERVY
                     GELREKLGTKVVPINLPIGSHTSFEGVVDLVNMKAYYQKGGDTVEAEIPEDLKAEAEK
                     YREEMIEAVVVADEEILERYFEGEEISAEEIKRCMRLAAAQKEVFPVLCGSAELSIGI
                     QPLMNMLVYGMPAPVAKVPANRGGEKIELDPASGQNAALVFKTLADPYVGKITLFRVY
                     SGAIKSDSQIFNATKGETERLGQLFFMQGKDQLATDEIVAGDIGGVAKLQYTETSDSL
                     CDKDKIVTFDPIKFPRPVLTLAVYPKGKADTDKVAAGLSRLSEEDPTISLEQSKETNE
                     FLLSGMGDLHLEIIASRLKKKFGVEMELVDPKVPYRETIRGTAKVEGKHKKQSGGRGQ
                     YGHVWLELSPLPLGEGFAFEDNIFGGAVPKQYIPAVEKGIRETMSEGVLAGYPVVDVK
                     VS"
BASE COUNT          972 a          749 c          975 g          756 t
ORIGIN      
        1 ggcggcggag gagcaaatat ccacagcttt acggaagcaa agaagcacag ggttcggctt
       61 gcagctctga acattggaga aggggaaaaa cctttggaag aagctgaaaa agcttatgca
      121 cagatcaaga cggacatcgt gtcagagaaa tctgcagtat tctacagaga gaaagccgga
      181 gtcggtgtcc gaagccgatc gtaaacgggc tttctgcggt ttccgagcgg agggaactaa
      241 acatggagac gtttgtagcg cgagtaaacg agcagaagaa acagctgaaa agacctctcg
      301 tggggctggc tttgggaagt ggagctgcga ggggaatggc tcacatcggc gttttgcagg
      361 tgctttttga agaggacatc cctatagact gcatcacagg aacatccatc ggatcccttg
      421 taggagcgat gatcctgtcg ggctggacgc cgcagtttct cggcaggttt gcccgagctg
      481 tgggaaagga gcacatggtc tcggttttgg attttgtgat tcccccgaaa acggggctga
      541 tcaacgggaa cagaataaag gttttgctcg aaagagtgtt gaaaataaag aatatcgaag
      601 agcttcccaa gccgtttgcg gcggtttcca ccgacattgc cactggagag cgagtggttc
      661 acgacagggg aagcctccta gaaggaatta gggctagcat ttcggtgcct ggagtcttcg
      721 tcccagcgga ggagcgcgaa agcggcaggc tccttgtgga cggaggggtg gtggatcccg
      781 ttcctttgga tgttttagtg gaggctggcg tggagattca gatcggtgtg aacatcctgc
      841 gcaaacccga tacggcggaa aaacctaaag atgtgatcga catcttgctt aattctatag
      901 acatcatggg agctcagatc tttgaactca aaagcacggg gaaggccata gtgctcgaac
      961 cgcttttggg acatcggttc ggcagcatgg actttcataa agctgaacct atgatagaac
     1021 tggggagaaa gtgcgcttta gaaaagcttc ctgagatcaa agaaaggcta agatcggaag
     1081 gggttttgta aatgctgtct gtagaagaag tggccggtgc tgcagcagga tgcgtccggt
     1141 gcagtatcag aaagaataca ccggaagtgg tcttcggaaa ggggagccca gacgctcgga
     1201 tcatggtggt gctggaagct cctgatgccg atgcggccaa agaggggaag cctatctcgg
     1261 gcgaagccgg ggagcttctg tataacattt tcgctgcatc gcatattaag ggtggagacg
     1321 tgtacgtaac caatctcgtc aagtgcaacc ctcccgaaga gagaacgcct actgaagaag
     1381 aaatcggctc ctgcaaaacg tggctggatc tggaaatagc tgccgttcag ccggactatc
     1441 tcatcctcat gggtgttgcg ccgctcagag cttttttcgg tgaggatgca gcaatacagg
     1501 agtttagagg taaatggcat agttttggtg aaataaaagt actgtgtact taccaaccca
     1561 aaaccctgct gaaaaaaccc gagtacaaga gaatggtttg ggaggacttt aagatgctta
     1621 tgagagattt gggtatctta aagccgtaaa ccggcaaaaa aaagaagaac tttacaagtt
     1681 ctgcaagttt ttcaaaccaa gcagagggaa agaggttttt ttgcagaaga tttgtaagga
     1741 tactctcatt gatcaattta gttatactgc cgaaagggga gagagttttg aagaagtacg
     1801 atacttccca gattagaaac gtcgctatca ttggccatgg tggaacaggt aagaccatgc
     1861 tcacagaggc catcctgttc catgccggcg ccaccaagag aaaaggatcg attagcgacg
     1921 ggaatacggt ttctgactac gatcccgaag agatcaaaag agggacgtcg atatcgacgg
     1981 ctttaactac atgccagtgg aaagatacta aaatcaacct gctggataca cctggatttt
     2041 ttgattttgt cggcgaagtt gtagcagctt tggaggtaac agaaggagcc gtcagcgttc
     2101 tttctgctac agactccctt ccagttggtt ttttcacaac ctggaaactg gcggatgaga
     2161 gaaagcttgc ccgcaccatt tttattaaca aaatggataa ggaaaacgct tcctttgaaa
     2221 gagtttacgg cgagctgaga gaaaaactgg gaactaaagt tgtgccgatc aacttaccga
     2281 taggcagcca cactagcttt gagggtgtag tagacctggt taatatgaaa gcctactacc
     2341 aaaagggcgg cgatacagtc gaagccgaga ttcccgaaga cttgaaagcg gaagcagaaa
     2401 aataccgcga agagatgatc gaagcggtag tagtagccga cgaggaaata ctcgagcgct
     2461 actttgaagg cgaagagatc tccgcagaag aaatcaagcg ctgcatgcgc cttgctgctg
     2521 cacagaaaga agtcttccct gtactctgcg gttcggcaga actgagtatc ggtattcaac
     2581 cattgatgaa catgctggtc tacggcatgc cggctccagt agctaaagta ccagccaaca
     2641 gaggcggaga aaagatcgaa ctagatcccg cttccggtca gaatgccgca ctggtgttca
     2701 agactttggc cgacccctac gtgggcaaga tcaccctgtt tagagtgtac agcggtgcta
     2761 taaagagcga ctcccagatc ttcaatgcca ccaaaggcga aaccgaaaga ctcggacagc
     2821 tcttcttcat gcagggcaaa gaccagttgg cgaccgatga gatagttgcc ggtgacatcg
     2881 gtggcgtagc caaactgcag tatacagaga catcagattc cctttgcgat aaagacaaga
     2941 ttgtcacttt tgatccgatt aagttcccga gaccagtact gactttagcc gtatatccca
     3001 aaggcaaagc cgatacggac aaagttgctg caggtctcag ccgcttgagc gaagaggatc
     3061 ccaccatctc cctggagcag agcaaggaga ccaacgagtt cctcctttcc ggtatgggcg
     3121 atctgcatct ggaaatcatc gccagccgct tgaagaaaaa gttcggcgtt gagatggaac
     3181 tggtagatcc taaggtgccg taccgcgaga ccattagagg cactgcgaaa gttgaaggca
     3241 agcacaagaa gcagagcggt ggcagaggcc agtacggtca cgtttggctt gaactctctc
     3301 cgcttccact tggtgaaggc tttgcatttg aagacaacat ctttggcggc gcagtaccaa
     3361 agcagtacat tcccgcagtt gaaaaaggca tcagggaaac catgagcgaa ggcgttcttg
     3421 caggatatcc tgtggtggac gttaaggtta gc
//
LOCUS       DAOSDA010000169         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_17428_length_3451_cov_4.046820, whole genome shotgun sequence.
ACCESSION   DAOSDA010000169 DAOSDA010000000
VERSION     DAOSDA010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17428_length_3451_cov_4.046820"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..821
                     /locus_tag="PLW82_04790"
     CDS             312..821
                     /locus_tag="PLW82_04790"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ46622.1"
                     /translation="MKRISFLGLGIALTAVATMAVQIPIPATKGYLNLGDSVIMVLAV
                     VFGPRFGFLVGSLGSALADVVTGYVYWAPATLIIKGVEGFLVGWFSGRSKQPPNHLKL
                     IWSFAVGAITMAVGYFIAGAIMYGGAAALADTPANLVQGIGSVVVALPLTYALKKLRF
                     LDREKKLDK"
     gene            841..1890
                     /locus_tag="PLW82_04795"
     CDS             841..1890
                     /locus_tag="PLW82_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOJ46623.1"
                     /translation="MHLVTLIPGDGIGPEITESVKEILSSAKAPVEFEEVQAGEGALE
                     SEGELLPLKTLQSLEKTKVGLKGPITTPIGKGFRSVNVSLRQIFNLYACVRPYRSMPG
                     IASRYENVDIVVVRENTEDLYKGVEFAPNSKETRTISELTGGAVSEDAAISIKAITAH
                     ASERIGRFAFEYAKKHGRKKVTATAKANIMKFTDGLFYDSVRKVAEEYPEIPYNEVLV
                     DALCMKLVQAPENFDVLVMSNLYGDLISDLVAGLVGGLGVAPGANIGDGMAIFEATHG
                     SAPDIAGKNLANPLGLLLSAVMMLEYLGEMDVARRITKAVETVLADGRYVTKDLCPEG
                     VTTSEMTKAIIKALD"
     gene            2066..>3451
                     /locus_tag="PLW82_04800"
     CDS             2066..>3451
                     /locus_tag="PLW82_04800"
                     /inference="COORDINATES: protein motif:HMM:NF016472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-dependent glucokinase/phosphofructokinase"
                     /protein_id="HOJ46624.1"
                     /translation="MRAAWAEKVRKAASQKINCRVFAAFNTNVDVVVHVNNENLNRIY
                     QKDPGILSLGLEEDFSRQADLIESPEEFISILLGAMKYGKSLYMLTTSEKLLSWLSQS
                     FTEAREILGGQAGIIANQISNLGAEAVVYSRLLSPQQAALFKDEVLAPAVRDNQLVVE
                     PVKQVANPNDECKVNWIFEYAKDVTVTLGDTTVTTPRANRVILATRPKNAIMSFADEM
                     MDHLPELGKHIDLAFMAGYHYISENNPDGRSYREFLDKALKELELLRQGNPKVAVHYE
                     YVPMKERNLEAETLAEIGNAIDSFGINENEIKLVLDDFDFQEERKELEKSERAYTLYR
                     AALPIFRKMNLKRLHVHNLGYYVVIVSKDYHISPEICREACLYASSVNALKAKYGGFA
                     EGERLPEAGQIELSEIGFKQLLGIKEEMDLPDEFLETGIYEGDDHYLLVVPAHVIQNP
                     VSTVGMGDTISS"
BASE COUNT          937 a          777 c          899 g          838 t
ORIGIN      
        1 cctagtaaag ccgtgtgact ctgggtttgg agtctttcga tctcatacta tttttatagc
       61 tttagcaaag ggagaaggtg gagttttcag gtccaccttc ctttgaaata tcagcaggcc
      121 gcaatatcgg acgacctgtg catgctctag cagccatcga tcagcacaaa ctgtatcgtt
      181 ggccatgata gcgcaggttg cagcgtcaaa gcaagctggc caattgcaag atcctgctgg
      241 cgtttccttt tattttcgac acgacacgtt ttgatgagcg ccctggaatc aatctgtaaa
      301 ggagtaagcg tatgaagcgg attagttttc tgggattagg tatcgcactt actgcagtgg
      361 caaccatggc ggttcagata ccgatacctg caaccaaagg atacctcaat ctaggagaca
      421 gcgttattat ggttcttgca gtagtcttcg gtcctaggtt cggcttttta gtgggttctt
      481 tgggctctgc ccttgcagac gttgtcaccg gttacgttta ctgggccccg gccactttga
      541 ttattaaagg ggttgagggc tttctggtag gttggttctc agggaggagt aagcaacctc
      601 caaaccacct gaagctgatt tggagctttg ctgttggggc cattaccatg gcagtggggt
      661 actttattgc aggagcgatt atgtacggcg gggccgcagc tcttgccgac acacctgcca
      721 acctagttca gggcatcggc agcgtggtcg tagcactgcc tctcacgtat gctctaaaga
      781 agctgcggtt tttggataga gaaaaaaagc ttgacaagta aacaagaaag gggtagagaa
      841 gtgcatcttg taacgcttat acctggagat ggcattggcc ctgaaatcac cgaatcggta
      901 aaggagattc tttccagtgc taaagctcca gttgaatttg aagaagttca agccggcgaa
      961 ggagctttag agtctgaagg cgaactttta ccccttaaga cgcttcagtc gttggaaaaa
     1021 actaaagtag gtctgaaagg tccgattacc actcctatcg gcaagggatt tcgcagtgtg
     1081 aacgtaagcc tacggcagat attcaacctc tatgcctgcg tacgtcccta caggagtatg
     1141 ccgggcatcg cttcaagata tgagaacgtg gatatcgtgg tggttaggga aaacaccgaa
     1201 gacctgtaca aaggcgtgga gtttgcaccg aactcgaaag aaaccaggac catcagcgaa
     1261 ctgaccggtg gagcggtttc tgaagatgcg gcaatttcca tcaaggcaat caccgctcat
     1321 gcttctgaga ggatcggcag gtttgctttt gagtacgcca aaaagcacgg tagaaaaaaa
     1381 gtaacagcaa cggctaaagc gaatataatg aagtttacag atggactttt ctacgactct
     1441 gtgagaaaag tggcagagga gtaccccgaa atcccctaca acgaggtttt ggttgatgcc
     1501 ctctgcatga agctggttca ggctccggaa aactttgacg ttttggtgat gtccaatctc
     1561 tacggcgacc ttatctccga tttggtggct ggtcttgtag ggggacttgg cgtcgctccc
     1621 ggtgccaata tcggcgacgg aatggcgatt ttcgaagcca cccacggctc ggcacctgac
     1681 attgcaggta aaaaccttgc caaccctttg ggtctccttc tctcagcagt gatgatgctt
     1741 gagtatcttg gagaaatgga tgtggcgaga agaataacaa aagccgtgga aacggttctt
     1801 gcagacggta ggtatgtaac taaagatctg tgtccagaag gcgtaaccac gtcggagatg
     1861 accaaagcca tcattaaagc attggactag cgttggcaaa gatcaaagct agttggtgaa
     1921 aactattatt ttcttatatt attctcgcat agcgtgtgta agtgtagtat actaaccatg
     1981 gcgatttaga agactatctg caacgagttt atggtggcag atagaggacg gaggaatgca
     2041 aatggaacag cctcaagttc aaaaaatgcg tgcagcatgg gccgaaaagg tccgaaaagc
     2101 agcaagtcaa aagatcaact gccgcgtgtt tgcggctttc aataccaacg ttgacgtagt
     2161 cgttcatgtg aacaatgaaa acctcaacag aatttatcag aaagatcccg gaatcctgtc
     2221 tttgggtttg gaagaagact ttagcagaca agccgatctg atcgaatcgc cagaagagtt
     2281 tatcagcatt cttcttggtg caatgaagta cggtaagtct ctgtatatgc tgacaacctc
     2341 tgaaaaacta ctcagctggc tttcgcagtc ttttacagaa gctagagaga ttctcggcgg
     2401 ccaggcgggc atcatcgcca accaaatcag caacctaggg gcagaagctg tggtttactc
     2461 cagattgctc tcccctcaac aggcggctct gttcaaagac gaagtgctag cgcctgcggt
     2521 cagagacaac cagcttgtag ttgaaccggt aaagcaggtg gctaacccca acgacgagtg
     2581 taaggtcaac tggattttcg agtacgccaa agacgtcacc gttactttag gtgatactac
     2641 agtcaccact cctagggcca accgtgtaat cctggctaca agaccgaaga acgccatcat
     2701 gtcttttgct gacgagatga tggatcatct gccagagctc ggaaagcata tcgaccttgc
     2761 cttcatggca ggttatcact atatttcgga aaacaaccca gacggacgca gctatcgcga
     2821 atttctcgac aaggcactaa aggaactcga gctcttgcgg cagggtaacc ccaaagtggc
     2881 cgtccactat gagtatgtgc cgatgaaaga aagaaacttg gaagctgaga cccttgccga
     2941 gatcggaaat gccattgaca gctttggcat taacgaaaac gaaatcaagt tggttctcga
     3001 tgacttcgac ttccaggaag agcgaaaaga gctggagaaa tctgaaagag cgtatacgct
     3061 gtacagagca gcactaccga ttttcagaaa gatgaacctg aagagactcc atgttcacaa
     3121 cctcggctat tacgtagtta tcgtttccaa ggactaccac atttcgccgg agatctgccg
     3181 ggaagcctgt ctctacgctt cgtcggtcaa tgctctgaag gcaaagtatg gcggatttgc
     3241 agaaggcgag agactgcctg aggcaggtca gatcgagctt tcggagatcg ggttcaaaca
     3301 gcttctaggc atcaaagagg aaatggatct tcctgatgag ttcttggaga ccggtatcta
     3361 cgaaggagac gatcactatc tcctggtagt gcccgcccac gtaattcaaa atcccgtaag
     3421 caccgtggga atgggagaca cgatctcttc t
//
LOCUS       DAOSDA010000170         3441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_17495_length_3441_cov_2.538689, whole genome shotgun sequence.
ACCESSION   DAOSDA010000170 DAOSDA010000000
VERSION     DAOSDA010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17495_length_3441_cov_2.538689"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLW82_04805"
     CDS             complement(<1..936)
                     /locus_tag="PLW82_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOJ46625.1"
                     /translation="MNYTMVFFILRKFIGMTAILMLLPLLVSVIYGEANFWAFLVPSL
                     TLLLLSFATRKMKPKNTTIYAKEGYLIVALAWLIVSLIGALPYYLSNVGLSYLHSLFE
                     AVSGFTTTGSSVLANVETMPYSLLFWRSFTNWIGGMGVLVFLLAILPQTNSRSVHVMR
                     AEMTGHQVGKLVSKVKNTVIILYGIYILMTLVLIGLLLLGRMPLFDSIVTAFATAGTG
                     GFAIKNLSIAFYRSPYIETIIGIFMILFGINFNLYFLAFTAHIKNALKSEELWWYLGI
                     ILVSTVAIACNIYSTYQNAVQSLRYGFFQVSSIITT"
     gene            complement(959..>1639)
                     /locus_tag="PLW82_04810"
     CDS             complement(959..>1639)
                     /locus_tag="PLW82_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOJ46626.1"
                     /translation="RIKDVMIIGGGKITDYLAQRLEELGVQMKIIEQDRQRCLELSRN
                     LKRAEIIHGDGANQDLLLEEGLERAGALISLTDMDEENIVISMYARSLGVEKIITKVN
                     SDAFSRILESLEIGSIVSPKETVVNRILPYVRAMHNAAESSNVETLYKIVGGQVEALE
                     FKVKENARYTRVPLKNLAIKPNILIAGIVRDDAVIIPSGDDFIQKMDSVIIVTTNTAI
                     GDLGDILD"
     assembly_gap    1640..1649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1650..2329)
                     /locus_tag="PLW82_04815"
     CDS             complement(<1650..2329)
                     /locus_tag="PLW82_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOJ46627.1"
                     /translation="MYIVIVGDGKVGTSLTKHLSAEGYDVAVIDKDPEVIEEAVNTYD
                     VIGVCGNGANYQVQLEAGVDKADIFIAVTSSDEINIFCCLTAKKLGARHTVARVRNPD
                     YRNQLIFMREELGLSMVVNPERDTAVEIARMISSPATITRDVFSRGRMELAELKIGET
                     NPLRERTLASLKQHNLKVLVCAVQRDDDVYIPSGNFTLKASDKIYVTGSPKNLNNFIE
                     FCGLGSTR"
     gene            2547..2828
                     /locus_tag="PLW82_04820"
     CDS             2547..2828
                     /locus_tag="PLW82_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOJ46628.1"
                     /translation="MFVGTAIVKLHAPWVHSLKEKRSEVQKLTKRVRNKFNVSVCEAD
                     MTDVHQTIVLGLGCVSSSKSHLDAALQEIIRFIEQNTDAEVFDVEVEII"
     gene            complement(2884..>3441)
                     /locus_tag="PLW82_04825"
     CDS             complement(2884..>3441)
                     /locus_tag="PLW82_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ46629.1"
                     /translation="ISMLFSLPIPLGPVHLLWINLVTDALPALALGVEQGEKDIMKQK
                     PRDPKEPILNSSAWGMIFFHAFVLAFSVLIAFLIGLTTRGSLQVARTMAFLTIICGEL
                     FRAFSCRSEKASIWKTGFFKNPLMVWAVVVTILLMVPLFAFPALGRAFSLAALSGQEW
                     LAIFGLSLLPLIFGEVSKMVAPDRR"
BASE COUNT          899 a          805 c          865 g          862 t
ORIGIN      
        1 ggtggtgata atagatgaaa cctggaagaa gccgtatctc aaggattgca ccgcgttttg
       61 gtaagttgag tagatgttgc acgcgatagc gacggtagat actagtatga tgcccagata
      121 ccaccagagc tcttcgcttt tcagagcgtt ttttatgtga gcggtaaaag ctagaaagta
      181 caggttgaag ttgatgccga acagaatcat gaaaatgccg ataatggtct cgatatatgg
      241 actgcggtaa aaagcgatgc tcaagttttt gatggcaaaa ccgcctgttc ctgcagtggc
      301 aaaggcagtc acaatgctgt caaaaagggg cattctccct agaagcagta accctatgag
      361 aacaagggtc attaagatgt agattccgta gagaattatc acggtatttt tgaccttgga
      421 cacaagcttg cccacctggt gtccggtcat ctccgctctc atcacgtgga cggatctaga
      481 gttggtttgg gggagaatag caaggaggaa aaccaaaacc cccataccac cgatccagtt
      541 agtgaagctg cgccaaaaca ataggctata gggcattgtt tcgacgttag ctagaacgct
      601 gcttcctgta gtggtgaagc cggagacagc ttcaaaaagg gagtgcaagt agctcaaccc
      661 cacgttggag aggtagtagg gtagagctcc aatgagcgac acgataagcc aggcgagcgc
      721 cacgatcagg tatccttctt tggcgtagat ggtggtgttt ttcggcttca tctttctggt
      781 ggcaaaggaa agaagaagca gggtgaggct gggaacgagg aaagcccaga agttggcttc
      841 tccgtagatc acgctcacta gtaggggaag cagcatcaaa atggccgtca tcccgatgaa
      901 tttccttaaa atgaagaaaa ccattgtgta gttcatgtgc aatacctctt agcttgagtt
      961 agtccaggat gtcacctaaa tcgccaatgg ccgtattggt agtcacaata atcacgctgt
     1021 ccattttttg gatgaagtca tcaccagatg gaataataac tgcatcgtcc ctgacaatcc
     1081 ctgcgatcaa aatgttgggt tttatagcaa ggttctttaa tggcacccta gtataccttg
     1141 cgttttcctt aaccttgaac tctaaagctt ctacctggcc gccgacgatc ttgtacaggg
     1201 tttctacgtt gctgctttca gcagcgttgt gcatagcgcg aacgtagggc aggatgcggt
     1261 ttacaacggt ttctttcggc gagacgatgc tgccgatctc caggctttct aggatcctgc
     1321 tgaaagcgtc gctgtttact ttcgtaatga tcttttccac acctaaggac ctagcgtaca
     1381 tggaaatgac gatgttttcc tcgtccatgt ccgtcaaaga gatgagagct ccagcccgtt
     1441 ccaaaccttc ttccaggagg agatcctgat ttgccccatc tccgtggatg atctctgccc
     1501 ttttaaggtt tctactcagc tccaggcagc gttgcctgtc ctgctcgatg atcttcatct
     1561 gtacgcctag ctcttctagc cgctgtgcca ggtagtcggt gattttacct cccccgatga
     1621 tcatcacatc tttgattctn nnnnnnnnna ttctagttga gcccagtccg cagaactcaa
     1681 tgaaattgtt gaggttcttt ggggatccgg ttacgtaaat cttgtcgctg gcttttaggg
     1741 taaagtttcc gcttgggatg tatacgtcgt cgtccctttg caccgcgcag accaaaacct
     1801 tcaggttgtg ctgcttcagc gaagctagag tcctctcacg aaggggattt gtttcgccga
     1861 tcttaagctc ggcaagctcc attctgcccc tgctgaacac gtctctagta atggttgcag
     1921 gggagctgat cattctagcg atttctactg cggtatcccg ttcagggttt accaccatgc
     1981 tgagacccag ctcttcccgc ataaagatca gctggtttct gtagtcggga tttctcaccc
     2041 gggcaacagt atgccttgct cccagtttct ttgcggtcag gcagcagaaa atgttgattt
     2101 cgtcggaaga cgttactgca atgaagatgt ccgctttgtc aacacctgct tctagctgta
     2161 cctggtagtt tgcgccgttg ccacagaccc cgatcacatc gtaggtgtta acggcttctt
     2221 caataacctc cggatctttg tctatgactg caacgtcata gccttcggcg gaaagatgtt
     2281 tggttaagga agtgccaact ttaccgtctc caacaatgac aatgtacatg aacttcccct
     2341 ccgcagcggt aagaatcaca ggccaatgcc tatgtaagtt gctcagtact atcataccca
     2401 tacccaggaa aaaaggaaat gcacaaaaaa tgtgctatac ttcaataata caatacactc
     2461 cgaaggaaac ctgtatctgg gaatcagctt tcctgaaaaa acgtctttag cacagctaaa
     2521 agactaaggg aaggggtcaa cacgcgatgt ttgtgggaac tgccatcgtc aagctgcatg
     2581 cgccttgggt gcattcttta aaagagaaac ggtctgaagt gcagaagctg acaaaaagag
     2641 tccgcaacaa gttcaatgtt tcggtttgcg aagcggatat gacagatgtc caccagacca
     2701 tagtgctggg gctcgggtgc gtatcatcaa gcaaaagcca cttggatgca gctctgcagg
     2761 agatcattag gtttatcgaa caaaacaccg atgcagaggt cttcgatgtg gaagtggaga
     2821 ttatatagga aagcaaagaa ggtgaccgaa aatgcggtca cctttcattt tccagattat
     2881 taactatctt cggtctggag caaccatttt ggaaacctcc ccgaagatca gcggcaagag
     2941 gcttagaccg aaaatcgcaa gccactcctg cccggagagg gccgctagtg agaaagccct
     3001 gccaagggcg gggaaagcaa acagcggcac catcaggaga attgtaacga ccaccgccca
     3061 taccatcagg gggttcttga aaaagcctgt tttccagatg gaagcttttt cagatctaca
     3121 gctgaaagcc cggaacagct ctccgcagat gatggtaaga aaggccatgg tgcgcgcaac
     3181 ctgcaggctg ccccgagtag tcaaaccgat gagaaaagcg attaggactg aaaaagccag
     3241 gacaaaagcg tggaaaaaga tcatgcccca ggcggaactg tttaagatag gttctttggg
     3301 gtctcggggc ttctgcttca tgatatcctt ttcgccctgt tcaactccga gtgccagtgc
     3361 tggaagagcg tcggttacca gatttatcca gagaaggtgc accggaccga ggggtatggg
     3421 cagggaaaaa agcattgaga t
//
LOCUS       DAOSDA010000171         3424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_17603_length_3424_cov_2.740873, whole genome shotgun sequence.
ACCESSION   DAOSDA010000171 DAOSDA010000000
VERSION     DAOSDA010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17603_length_3424_cov_2.740873"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLW82_04830"
     CDS             <1..369
                     /locus_tag="PLW82_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46630.1"
                     /translation="VPDIRDGVEPANLHRDHKAVKIWNFRFGKDFIPYFLLVFFLIAA
                     YIVSWLPQATITILTIGDNTGSVPLWGILIITILASIPITLLLYDGWWYWYKKVIPLW
                     IKDEETLKKAEEKIIEIANN"
     gene            398..1540
                     /locus_tag="PLW82_04835"
     CDS             398..1540
                     /locus_tag="PLW82_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46631.1"
                     /translation="MSRKTKLLLVLVLVLAVGSLVFANIWIDEVTAVKAENLKSYLSL
                     TQEYQDNNWYKVSSKVNKLVEAEAATVGGGAVVRDALNASGGKVVSGFDKEGAYIEWE
                     FDAPARGQYEIWFKYRLLSADAEPVVVDITVDGKHLFTEMKTLHFNSVKYASGVETLE
                     RIEPASDAGNGVPFGFIMEKGKHRIRITNISGEFELDYIKFCGPVKHFVFTQGEKWSN
                     ANGEYEIVKGVAASRGKGVKSSDVPGEWIEWKIDVPEDGLYNIIFRYTQGQRGVESVR
                     HIQVNGQTLFESWENVVFYPTDGWSEKRNDWRERRLEDPESRQPYYIYLPKGEVTIRM
                     ETVSGPLNIDYVGIITRDGEIKGELNFWDYLRMLWHQLIGFILSFN"
     gene            1553..2107
                     /locus_tag="PLW82_04840"
     CDS             1553..2107
                     /locus_tag="PLW82_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46632.1"
                     /translation="MSNLESKYNVLATQTSKKIRRKCGFQAGMAVVLAVIAAYVVFGA
                     AVPTIVTAFRTIAAAGAVELAEKSNALSGGVKFLLLVLTALYIGVLGVIAAENRAKDK
                     QIKYLGLETYSDMYVFVAGMLLGLLVTMLIVKFAWWSIVVFILSPGLAILCRAIWWWA
                     YARLLFKKSDLDAEATMGKYESLR"
     gene            2309..2470
                     /locus_tag="PLW82_04845"
     CDS             2309..2470
                     /locus_tag="PLW82_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46633.1"
                     /translation="MIFQLTDEDILKVCSQCEHYDDGQCKKHEKPVAQLRRCEVWDSV
                     YREKCIRMR"
     gene            2635..2796
                     /gene="scfA"
                     /locus_tag="PLW82_04850"
     CDS             2635..2796
                     /gene="scfA"
                     /locus_tag="PLW82_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HOJ46634.1"
                     /translation="MNNNKIKGKHIITVQAWGSKMKVEEVGCGECQASCQSACKTSCT
                     VGNQVCLRK"
     assembly_gap    2878..2887
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2888..>3424
                     /locus_tag="PLW82_04855"
     CDS             <2888..>3424
                     /locus_tag="PLW82_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOJ46635.1"
                     /translation="SLHQLDDEAFKIVEAILNSKTESVSCADLRFVLPDIDSATLEEV
                     FAELQELKSHGQIFSDDELVTDLPKLPKGLKALCLNIAHDCNLACGYCFASQWDFGGK
                     KALMPYEVAVKAVDMLIENSGTRRHLEIDFFGGEPLLNFEVIQDLVAYIRQREKETGK
                     IFTLTLTTNAILLDDEKIA"
BASE COUNT          941 a          700 c          878 g          895 t
ORIGIN      
        1 gtaccagaca tccgagatgg ggtagagccc gccaacctgc accgggatca caaagcagtg
       61 aagatttgga acttccgatt tggcaaggac ttcattcctt attttttgct ggtgttcttt
      121 ttaattgcag cgtatatcgt gtcttggtta ccgcaagcaa cgataaccat actaacaatc
      181 ggtgacaaca caggttccgt tcctctatgg ggaatcctca ttattacgat tttagcgtca
      241 atacctataa cactgctttt gtatgacggt tggtggtatt ggtacaagaa agttattcca
      301 ctttggatca aagatgaaga aactcttaaa aaagcagagg aaaaaatcat cgagattgcg
      361 aataactaat tgttaaccac tagtaaggag tgatccaatg tcacggaaaa ctaagctttt
      421 gttagtgtta gttttagtgt tagctgttgg ttcgctggtc tttgcaaaca tctggatcga
      481 tgaagtaaca gctgtaaagg ccgagaatct caagagctat ctatcattaa ctcaagagta
      541 tcaagataac aactggtaca aggtcagcag caaggtcaac aagcttgttg aggccgaagc
      601 agccactgtg ggaggcggag cagtggttcg cgatgctctc aacgcctctg gtgggaaagt
      661 tgttagcggt ttcgacaaag aaggtgcata tattgaatgg gagtttgatg cacctgctag
      721 aggtcaatac gagatctggt tcaagtacag acttcttagt gctgatgccg aaccagttgt
      781 ggtagatatt actgtggacg gaaagcatct gttcaccgaa atgaaaactc tgcatttcaa
      841 ctctgttaag tatgcctctg gagtcgaaac tttggaacgc atcgaacccg catctgatgc
      901 aggtaacgga gtcccctttg gctttattat ggaaaagggc aagcatagaa ttcgcatcac
      961 caacatcagc ggcgagttcg aactggacta cattaagttc tgcggtccgg tgaagcattt
     1021 cgtcttcact cagggtgaaa aatggtcaaa tgctaacggc gagtatgaaa tcgtcaaagg
     1081 tgttgccgct agccggggca aaggcgttaa gtcatcggat gttcccggcg agtggattga
     1141 gtggaagatc gacgtgcccg aagacggact gtacaacatc attttccgct acactcaagg
     1201 acagcgcgga gtggaaagcg taagacacat ccaggtcaat gggcagactc tctttgagag
     1261 ctgggagaat gtagtcttct atcctacaga tggatggtct gaaaagagaa acgattggag
     1321 agaaagaagg ctcgaggatc ccgaatccag acaaccatac tacatctatc tgcctaaagg
     1381 tgaagtcaca attcgcatgg agacagtctc tggacctttg aacatcgact atgtaggcat
     1441 tatcactaga gacggagaaa tcaaaggcga acttaacttc tgggattatc tcagaatgct
     1501 gtggcatcag cttattgggt ttatcctgag ctttaactga ggaggttagt cgatgagcaa
     1561 cttagagtca aagtacaatg ttcttgcaac acaaaccagc aaaaagatcc gcagaaagtg
     1621 cggtttccaa gcgggcatgg ccgttgtttt ggctgttatc gccgcatatg ttgtattcgg
     1681 cgctgcagtg ccaacgatcg taactgcttt tagaacaatt gctgcagcag gtgctgtaga
     1741 actggccgaa aaatcaaatg cactgtctgg tggcgtgaag ttcctacttc tagtcttgac
     1801 agctctgtac attggagttc tcggcgtaat cgctgctgaa aaccgtgcaa aagacaaaca
     1861 gatcaagtac ttgggtttgg agacctattc cgacatgtac gttttcgtag ccggcatgct
     1921 gcttggcctg cttgtaacca tgctgatcgt gaagttcgca tggtggtcga ttgttgtctt
     1981 tattcttagc ccaggtttgg caattctctg ccgcgctatc tggtggtggg catacgctcg
     2041 cttgctgttc aagaagagcg acctcgatgc tgaagcaaca atgggcaagt acgaaagcct
     2101 tagatagtct gtgcgagcaa ccttcgggtt gctctttttt tgatttggag caggggaaac
     2161 cggtgtatcc ggaaggaatc acccccggtt tagcgaaaga agccaataag acgagaacta
     2221 ctctgccaag acttggatat tatagaaagc agcgatcttc taccgaacgt tctatttggc
     2281 agccactagt ccacggggag ggagttgcat gattttccag cttactgacg aggatattct
     2341 gaaagtctgc agccagtgcg aacactacga cgatggacaa tgcaaaaagc atgaaaaacc
     2401 ggtggcccag ttaagacgct gtgaagtttg ggatagcgtt tatcgggaaa aatgcatcag
     2461 aatgcgctag tcttgggaga aggcctagcc ttggagctag gtcttttttc tttcacattg
     2521 aagggggcat ttttagctag ttgggcagga tatatcacca ttttgtggaa taacactatg
     2581 tgaatagctg cgagcgaaag ctgacgttag gcttagtttg aggaggagtt tagcatgaat
     2641 aataacaaga ttaagggaaa acatataatt acagtacagg cttggggttc caaaatgaag
     2701 gttgaagagg ttgggtgtgg cgagtgtcaa gcttcctgcc aatcggcttg caagacttca
     2761 tgtactgtag gtaatcaggt ttgcctgcgt aagtagggca aatatttatg acggcaatac
     2821 atgtaggaaa cccgaacaac acccatttgt tcgcagttaa tgggtttaag tttgcatnnn
     2881 nnnnnnnttc gctacaccag cttgacgacg aggcctttaa gatcgttgaa gccattctaa
     2941 actccaaaac cgagagtgta tcctgtgctg acttgcgctt cgttttgcca gatatagaca
     3001 gcgcaactct cgaggaggtt tttgccgagc ttcaagagct taaaagccac ggacagattt
     3061 tttccgatga tgagcttgtg accgacctgc caaagcttcc caaagggctt aaagcgctct
     3121 gtctgaatat cgctcacgac tgcaacttgg cttgcggata ctgtttcgct tctcagtggg
     3181 atttcggcgg caagaaagcg ctcatgccct atgaggttgc ggtaaaggca gtagacatgc
     3241 tcatcgagaa ctccggcact cgtcgccatc tggagatcga cttttttggc ggcgagcctc
     3301 ttttgaactt tgaagtcata caagatttgg tagcttacat taggcagaga gaaaaagaga
     3361 cgggcaagat ctttactcta actctgacaa caaatgcaat actgttggat gatgaaaaga
     3421 ttgc
//
LOCUS       DAOSDA010000172         3423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_17607_length_3423_cov_3.618468, whole genome shotgun sequence.
ACCESSION   DAOSDA010000172 DAOSDA010000000
VERSION     DAOSDA010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17607_length_3423_cov_3.618468"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..695)
                     /locus_tag="PLW82_04860"
     CDS             complement(123..695)
                     /locus_tag="PLW82_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HOJ46636.1"
                     /translation="MWLYQRMLQYPVKVSGPNPALAKNIITQYGGPDGELSASLRYLT
                     QRYSMPLKETKGLLTDIGTEELAHMEMIAALVYNLVKDASLAEIKKAGLDPYYADHDR
                     ALYFVNASGTPWTAAYIQSKGDPITDLTEDMAAEQKARSTYEYLINLSDDPLVTDALR
                     WLREREIVHFQRFGEALVRTQEYLDRKHYY"
     gene            complement(696..965)
                     /locus_tag="PLW82_04865"
     CDS             complement(696..965)
                     /locus_tag="PLW82_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HOJ46637.1"
                     /translation="MSKQKEALLREIQELQFTTVELQLFLDTHPDDQRALADYVEYSK
                     KLQELIAQYSKMYGPLIGYGVTSEELDKGYRWIDSWPWEDLGGKR"
     gene            complement(958..1107)
                     /locus_tag="PLW82_04870"
     CDS             complement(958..1107)
                     /locus_tag="PLW82_04870"
                     /inference="COORDINATES: protein motif:HMM:NF022460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HOJ46638.1"
                     /translation="MTDQNRLARAFVIIQKYNKRYSPEVGLSRGTIFPELYMPYLKGR
                     TKQDE"
     gene            complement(1576..2178)
                     /locus_tag="PLW82_04875"
     CDS             complement(1576..2178)
                     /locus_tag="PLW82_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ46639.1"
                     /translation="MNLVIEMLIDNVKTLAELDSVMDLVFSVFPYLPTDDNKYSLSFW
                     AGKMNELPELLLYAKDGEVICGSVFAWVDNGGVTVAHCCVAPAYRGQGIGRDLMLEIE
                     RRVKALGYHSIVLGSVEGAERFYEKLGYKGSLLIQSEDHSIDQLLSLNDKYEVIYTNV
                     YEGTVNQVCLRLSSPDRELQRLYEKTFPSCTTQMVFGKSF"
     gene            2398..>3423
                     /locus_tag="PLW82_04880"
     CDS             2398..>3423
                     /locus_tag="PLW82_04880"
                     /inference="COORDINATES: protein motif:HMM:NF027473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4855 domain-containing protein"
                     /protein_id="HOJ46640.1"
                     /translation="MSERGYFPPLTEESAGIRDLMLIYTNYGVNWNVKDALSYVAYLD
                     NNANQNKIKDWFFDSYLFLALKSNNDRAFDSPARATPAIKEDWQWWLDDLFRPNAQIS
                     VFNEAYKIANQHLPEKRKGKIYIMIPNPMVQITEFGEVDGENLDFSSKDATEATRQRF
                     KAVEWFVETFLEMFEKANLDQLELAGFYWLEEMIHANPGEDDLVRQVSKYLHDKGFKF
                     CWIPWFRAKGHDRWEEVGFDFCIHQPNYMFRADVPISRFADVTSDAKKYYQGIEIEAF
                     GKVFESKEWRERFYDYLRAGVTYGYMKDVIHGYYQDTHVFAISAYSDDPEIREIYDNV
                     YKFVQGTY"
BASE COUNT          890 a          811 c          816 g          906 t
ORIGIN      
        1 aactacccga caaatcccgg ctactctcaa ggacagtacg gccagcaagg ctacgcttcg
       61 cagtataccc agggcaacca aaataggcag taaaatgggg agcattgctg ctcccttttg
      121 cattagtagt agtgtttgcg gtcgagatac tcctgagttc tcaccaaggc ctcgccaaat
      181 ctctggaagt gaacgatctc cctttccctg agccatctca gggcatcggt taccagtggg
      241 tcgtctgaga ggttgatgag gtactcatag gtggacctgg ccttctgctc agcggccatg
      301 tcttcagtga ggtcggtaat gggatcgccc ttggactgaa tgtacgctgc agtccagggg
      361 gttcctgatg catttacaaa gtacagtgcc ctgtcgtggt cagcgtagta ggggtcgagg
      421 cctgcctttt ttatctcggc cagtgaagcg tctttgacca agttgtagac cagagctgct
      481 atcatttcca tgtgggccag ttcttcagtt ccgatatctg taaggagtcc tttggtttcc
      541 ttcagcggca tgctgtagcg ctgagtgaga tacctcaagg aagctgacag ctcaccgtcg
      601 ggtccaccgt actgggtgat gatgttctta gcaagagcgg ggttcgggcc gctgaccttt
      661 acagggtact ggagcattcg ttggtagagc cacatctatc gtttcccccc taagtcttcc
      721 caaggccagg agtcgatcca cctgtaacct ttgtccagtt cttcagacgt cactccgtag
      781 ccgataagag gtccgtacat tttcgagtac tgggctatca gctcctggag ctttttcgag
      841 tactctacat agtctgccaa agctctttgg tcatcgggat gggtatctag gaagagctgc
      901 agctctacgg tagtaaactg aagctcctgg atttcccgaa ggagagcttc tttttgctta
      961 ctcatcttgt tttgtcctcc ctttaaggta gggcatatag agctcgggaa aaatcgtgcc
     1021 acggctcagt cctacctcag gggaatagcg tttgttgtat ttttggatga taacgaaagc
     1081 gcgtgctaac cggttttgat cagtcatatt tcacacccct tcgctctagc atatgagtcc
     1141 ttgaaggatc tgtaatgcag tggaagtact agttgcttaa agtccaggga aacaggaaaa
     1201 atggtttaaa tgaaagcgcc cctgtaagag gcgcttttct acatctacgg tttactttcg
     1261 tgccgatggc gactttcgag cattcggtgt tgttagttca gatcaagcga ggtgtcaagt
     1321 tttgccacgc aacaccccag agtcttttaa cgtgtagttt cctttagctt cagtcaatgc
     1381 tctttcccgg gaaaagaact taacgtttac agcagtctct gaacgattga aaaatagctt
     1441 tcacacctat catcgtttgg actggatcca gcacaaatag gcatttcaaa acccttctcg
     1501 tactgtagta gtacgagaaa ggccgagcaa acaccaaact gattcttttg catgtactat
     1561 ctgtcgggtt tgttctcaaa aactctttcc gaagaccatt tgtgtggtac aactggggaa
     1621 cgttttctcg tagagcctct gtagctcgcg gtctggagaa gagagcctta aacagacctg
     1681 attgacagta ccttcataga cgttggtgta aatcacttcg tacttgtcgt ttagagacaa
     1741 taactgatct atgctgtggt cttcggactg aatgagcaga gaacctttgt agccgagctt
     1801 ttcgtagaat ctctctgcac cttcaacgga acccagaaca atgctgtggt aacccagcgc
     1861 tttcacgcgc cgttctattt ccagcattaa atctcttcca ataccttgcc ccctgtaagc
     1921 tggagcaacg cagcaatgag caacggttac tccgccattg tcaacccatg caaatactga
     1981 gccgcaaatg acttcgccat ctttagcgta taagagaagc tcggggagct cattcatttt
     2041 gccggcccaa aaacttaagc tatatttatt gtcatcggtt ggtaggtagg ggaagaccga
     2101 aaacactaaa tccattacag aatccagctc agctaaagtc tttacgttgt ctatgagcat
     2161 ttctatcacc aagttcactg tacgatgttt tcttttctgc tgtcaacggg cgaactccta
     2221 tccgaaacga gttttcaaaa tatcagagaa taggatcaac ctcagttcat acagtctctc
     2281 cgctatagtg gttagttcgc taatcgtaca gtgagattga gagctgaaac aaacggcagg
     2341 aatgcagaaa gtattgtgga agaaacctac taaagacaag tatagggggt taatccgatg
     2401 agtgaaagag gctacttccc gccgctcaca gaagagtctg caggcattcg cgacctgatg
     2461 ctgatctaca ccaattacgg agtcaattgg aacgtaaagg atgcgctgtc gtatgttgcc
     2521 tacctcgata acaacgcaaa tcaaaacaaa atcaaggact ggttcttcga ttcataccta
     2581 tttttagcgc tgaaatccaa caatgatcgc gcattcgact cacctgcaag agccactcca
     2641 gccatcaaag aagactggca gtggtggctg gacgatcttt tcaggccaaa cgcccagatt
     2701 agcgtgttca atgaagcgta caagattgcc aatcagcacc ttcctgaaaa gaggaagggt
     2761 aaaatctaca tcatgatacc caacccaatg gtccagatca ccgagtttgg tgaggttgat
     2821 ggcgagaact tggatttctc ttcaaaagat gctactgaag ctaccagaca gagattcaag
     2881 gctgtggagt ggtttgtaga aaccttcctg gagatgttcg aaaaggctaa tctggatcaa
     2941 ctggaacttg caggcttcta ctggcttgag gagatgatcc atgccaaccc aggcgaagac
     3001 gatctcgtgc ggcaagtgtc gaagtacctc cacgataaag gtttcaagtt ctgttggatt
     3061 ccctggttca gagctaaagg tcacgataga tgggaagaag tcggctttga tttctgcatt
     3121 caccaaccga actatatgtt tagggcagac gttcctatct cccgttttgc cgatgtcact
     3181 agcgatgcca aaaaatacta tcagggcatc gaaatcgaag ctttcggtaa ggtctttgag
     3241 tcaaaagagt ggagagaacg cttctacgac tacctaagag ctggcgtcac ctacggctac
     3301 atgaaggatg taattcacgg ctactaccaa gacacccacg tctttgcgat tagcgcctac
     3361 agcgacgatc cagaaatcag ggaaatttac gacaacgtct acaagtttgt tcagggaacc
     3421 tac
//
LOCUS       DAOSDA010000173         3421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_17620_length_3421_cov_3.379085, whole genome shotgun sequence.
ACCESSION   DAOSDA010000173 DAOSDA010000000
VERSION     DAOSDA010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17620_length_3421_cov_3.379085"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..761)
                     /locus_tag="PLW82_04885"
     CDS             complement(99..761)
                     /locus_tag="PLW82_04885"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HOJ46641.1"
                     /translation="MKKLYAAVYFLMMLSVAVTAIFLTLMPDTVPMHYNIKGEIDRYG
                     SKYENLIMPVSMILLGVLFLLLARHFQKRQQLENQRVVLASGSVLLGFLNVLFAYFLR
                     NSASFAPNLTDTTTDSIIFQITTIGLGFMLMILGYLMPQTSLNGIVGLRTVWSMKNER
                     VWQKSQKAGGIIMSACGAASMLLGAFLQGISSLIGAVVLLLVGTIFAVAVSYQIFLKD
                     QK"
     gene            1042..2298
                     /locus_tag="PLW82_04890"
     CDS             1042..2298
                     /locus_tag="PLW82_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46642.1"
                     /translation="MLSAFVSAKTLTVWLTGVNNEVLNIYRDLVEEKFTPITGIEVEF
                     TNLSWGDFENRFILAAATGDAPDVGGMGPLFAPELGIRGAVIDLRATFPDFDEVVKNL
                     YPAVFRSLSYQGSVFGVHFDANFAMTGFQRDDILQQHGIGSLDTWDDVRKALPKLQAN
                     GSNLSLAWQLTTDLYEDVNMFMWQHGGDDYTPDLKASGYDQPEAIRGFTEYVELYTKH
                     GIDRASPQIQAFADGSLFYFIQQPPWYSNLLVAHPQLQGKWSVVQVPGTVRDGVLHRE
                     SSVAGNALAIFRSSNMKEEAWSFIKWIISEPIQVEIAKRTLASSAGRLVLPADLNALA
                     KVDLPERDIEVYKKALAEGTTSIYGLVAPRHRRRYLQFAAEEAILLNVSPEEALVKYA
                     EEHNFEIKKKEVEYARFIQKLLEEQR"
     gene            2366..3013
                     /locus_tag="PLW82_04895"
     CDS             2366..3013
                     /locus_tag="PLW82_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46643.1"
                     /translation="MKGFATFSNALVMLEMKNPERSRIDKLFKAKVVLLGALTGALTP
                     IIVCLPLLLYLAVGFVYSSAAEFLVPGVTGFEGISFSQVASENAVFFAGIMKLILKYT
                     VLPSLVTGILDVYLRSVSLKDPKSEKLPNKLYTRFAAPIAVIYIPLIVLTWGAREAMF
                     GALGLLFLDFVIALFLGFIYRCALLVTMDRVLQVALKMQSFFTRLSARKLTGTVQ"
     gene            complement(3170..>3421)
                     /locus_tag="PLW82_04900"
     CDS             complement(3170..>3421)
                     /locus_tag="PLW82_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOJ46644.1"
                     /translation="HGEMPHGGFVIRSGETADGSSHVVEFSLKLASNPAFTSSVLIAY
                     ARAAYRMNEEGQLGAKTVFDVAPAYIAPKPNEILRKDLL"
BASE COUNT          923 a          787 c          835 g          876 t
ORIGIN      
        1 aaaaatccaa aaggccccaa gaaatccctg ggggtttttt ctatgggggc taggggatcc
       61 tatagaattg gggtatcgaa gcccatgtgc gatcactatt atttctgatc cttgaggaag
      121 atttgataag atactgcaac agcgaatatg gtcccaacta aaaggagaac cactgcaccg
      181 atcaacgagg aaatcccttg gaggaacgca cctaaaagca tcgacgcagc accacaagct
      241 gacatgataa tgccaccggc tttttgggac ttctgccaga ctctctcatt tttcatgctc
      301 cagacagtgc ggagtccaac aatgccgtta aggctggtct gcggcatcag gtagcctaaa
      361 atcatcaaca tgaatcccag acctatcgta gtgatctgga agataatgct atcagtcgta
      421 gtatcagtca agtttggggc aaaggaggcc gaattccgca agaaatacgc aaaaagtacg
      481 ttcagaaagc ctaagaggac tgagcctgag gccaaaacaa ccctctggtt ctccaattgc
      541 tgtcttttct ggaagtgtcg tgccaataat agaaacagga cccctagcag gatcattgaa
      601 accggcatga tcaggttctc gtacttactg ccgtaacgat cgatctctcc cttgatgttg
      661 tagtgcatgg gaaccgtgtc gggcattagt gtaagaaaga ttgcggttac agcaacagac
      721 agcatcatta agaaatagac tgcagcataa agttttttca atccaccagc cccctcgaac
      781 tgcaaatgcc agatttgcag gcttaatctc taattatatt atagtgcatt tccgggataa
      841 accgaattcc agagtagcat cgtccatacc cacctccaaa acagttcagc agcttacaag
      901 gagagataac acaggcattt tactgaaatt aacaaagaag ggaaacaact taatgtgacc
      961 gaagtacctg ttatagagca tttgggagaa agggtgagtt tagtgaggac caaaagacta
     1021 gcactaatat gtcttattat tatgctgtct gcctttgtgt cagctaaaac tctgacagtg
     1081 tggctgactg gtgtgaacaa cgaagttctg aacatctaca gagatcttgt tgaggagaag
     1141 ttcaccccca tcacaggaat tgaagttgag ttcaccaacc tgagctgggg agacttcgaa
     1201 aaccgtttta tcttggccgc tgctaccggt gatgctcctg atgtgggcgg tatgggacct
     1261 ctatttgcac cggaactggg gatcaggggt gcagttattg acctgcgggc aacattccca
     1321 gatttcgacg aagtagtaaa gaacctctat cctgcagtgt tcagatcttt gagttatcag
     1381 ggatcggtgt tcggcgtcca cttcgatgcc aacttcgcca tgaccggctt ccagagagat
     1441 gatattctgc agcagcacgg aattggcagt ttggatactt gggatgacgt gagaaaagcg
     1501 ctccctaaac tccaagcaaa cggcagcaat ctttctctgg catggcagct gaccaccgac
     1561 ctttatgaag acgtcaacat gttcatgtgg cagcacggcg gagacgacta cactccggat
     1621 ctcaaagcat ccggttacga tcagccagaa gccattagag gtttcaccga gtacgtcgag
     1681 ctgtacacaa aacacggcat tgacagggct tcacctcaga ttcaagcttt tgctgacggg
     1741 agtctgtttt acttcatcca gcaaccacct tggtactcca acctgcttgt tgcacatccg
     1801 cagctgcagg gcaagtggag cgtagtccag gttcccggta ccgtgcgcga tggagttctt
     1861 cacagagagt cttctgtagc tggcaacgct ttggcaattt tccggtcttc caacatgaaa
     1921 gaagaagcgt ggagctttat caaatggatc atttccgaac caattcaggt tgagatcgcc
     1981 aaaaggacac ttgcgtcctc tgcagggaga ttggtgcttc ccgcagatct caatgctctt
     2041 gcgaaagtcg atttgcctga aagagacatt gaagtttaca agaaagcgct ggcagagggc
     2101 accacttcga tctacgggct ggttgcacct agacacagaa ggcgctatct gcagtttgct
     2161 gcagaagaag ccatcctgtt gaacgtgtcc ccagaagaag cgcttgtaaa atatgccgaa
     2221 gagcacaact ttgagatcaa gaaaaaagaa gtcgagtatg ctcgattcat tcaaaaactg
     2281 ctagaggagc agcgttagta gtatgtatga ggtggggata actgtgtccc caccttttga
     2341 ctatgtaaaa agaggaggaa attcgatgaa aggtttcgct actttctcca acgcgctggt
     2401 catgctcgag atgaaaaatc cagagagatc caggatcgac aagctgttta aggctaaagt
     2461 ggttctgctt ggcgcactca caggagcttt aacaccgatc atcgtgtgtc taccactgct
     2521 gctgtaccta gctgtgggtt ttgtctattc gagtgccgca gagtttttag tgcctggagt
     2581 gacagggttt gaaggtattt cctttagcca agtggcaagt gaaaatgcgg tctttttcgc
     2641 tggcatcatg aagctgattc tgaaatacac tgtactgcca tcgcttgtca caggcatcct
     2701 tgatgtctac ctgcggtctg tctctttgaa agatccaaag tcagaaaaac tcccgaacaa
     2761 gctttacacc cgctttgctg cccctattgc ggttatctac ataccactta tagttctgac
     2821 atggggtgct agagaggcaa tgtttggtgc actagggctt ctctttttag attttgtcat
     2881 agcactgttt ttgggtttca tataccgctg cgcactgctt gttacgatgg atagggtact
     2941 tcaggttgcg ctaaagatgc agagcttttt taccagacta tctgcaagga agttaaccgg
     3001 caccgttcaa tagtgtttat gctcgaaaca cacactcgga gattaacaag tggatctgtg
     3061 ttaagcgata ggtggccatg aaaaacaaaa ccgcctacac cctataatct ggatcaagag
     3121 cgagaaaagc agaggctgaa ctgttcgttc agcctctgcc aattttaact tacaggaggt
     3181 ctttcctgag tatttcattt ggttttgggg cgatatatgc tggggccacg tcaaagacgg
     3241 tcttggctcc tagctgccct tcttcgttca tgcggtatgc agcccttgca tatgcaatca
     3301 gtacgctgga ggtaaaggct gggttggatg caagtttcaa ggagaactcc acaacatggc
     3361 tgcttccgtc ggcagtctca ccgcttctaa tcacaaagcc accatgaggc atttctccgt
     3421 g
//
LOCUS       DAOSDA010000174         3414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_17672_length_3414_cov_3.751712, whole genome shotgun sequence.
ACCESSION   DAOSDA010000174 DAOSDA010000000
VERSION     DAOSDA010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17672_length_3414_cov_3.751712"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..697)
                     /locus_tag="PLW82_04905"
     CDS             complement(155..697)
                     /locus_tag="PLW82_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ46645.1"
                     /translation="MILVRLSVKLIARTALLLALTLVIQLGGFPQPITGPGVNLMLIL
                     SAVFAGPVAAVIVGCLTPVIAFIRGIMGFAPLVPYIAVANAVYVLAFYFTSYFLQKAF
                     AKKERALLSIGFDAVGILLGAILKFAVLSSAVRFFVQAPPKMVQAMQVPQLITALTGG
                     AIALVVASALKKTGALKKAQ"
     gene            902..1627
                     /locus_tag="PLW82_04910"
     CDS             902..1627
                     /locus_tag="PLW82_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46646.1"
                     /translation="MINAQVVLSPKCSKRLIAKAVLESDYFKRAIEKGIVVLSLGTTN
                     AFIYEEFTGNKIAKEKYSVGIITDVLTVSESAGRIPALVLKRGEVLDIPWREALPEMD
                     QNSLFIKGANAYDITGLAGVLVGGEAGGTIGQALGTLVVNKVPLMIPVGLEKRVPSVK
                     EAAQLLSEPHVETKNMNVGMFVVTNGLIIDEIRAFEILFGLEAALIAAGGFPGFEGSV
                     VINLKGTEAKVVEALELVESLKL"
     gene            1678..2943
                     /gene="murA"
                     /locus_tag="PLW82_04915"
     CDS             1678..2943
                     /gene="murA"
                     /locus_tag="PLW82_04915"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOJ46647.1"
                     /translation="MDKIVVQGGYPLYGEVSINGSKNAALAVLVGAALGDEPCHVDNV
                     PHYTDIYDIIEILKDLGATVEWTGRSSVVIDGSKLNNPVAPYELVRKLRGSFYVAGLL
                     LAKIGYCEVPLPGGDPFSSRPVNFHLKGFEALGAEVTVEHGYVKAKADKLVGTTIYVG
                     RSSVGATVNLMLAASRAEGITTLENAAREPEVVNLALQLNSMGAKIRGAGTNIITIEG
                     VDYMKTARHEIIADRLEAGTFLIATSMAGGEVVLNDVVPEHLGSLISKLRESGVEIKD
                     DGGILTVKAPVGRRPIATDIETTPYPGFATDYQSMFAAYMTKAVGVSIVKETIFDRFA
                     YVDELARMGAEIKVEGDTAIIRGVERLTGAPVEVKDIRGGAALIIAGLAAEGKTILDG
                     VYHIDRGYEHLEEKLASLGAKIERIKDPS"
     gene            2981..>3414
                     /locus_tag="PLW82_04920"
     CDS             2981..>3414
                     /locus_tag="PLW82_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ46648.1"
                     /translation="MNKVIVIGSIHLDLVSRVEEIPQRGETVMGGDLNYFYGGKGANQ
                     AVAAAKQGACSYMIGKVGNDQFAQSLKDNLKNNGVNTDYVSVDPGRPSGIALIAVSKS
                     GENSIIVAKGASGALSFEEVVQAENLFSSGDYVLFQLEIPLET"
BASE COUNT         1009 a          700 c          871 g          834 t
ORIGIN      
        1 tcggataagc ataatgataa ctgaggaatt tacacactaa tttggacttg atccaagacc
       61 ggatgtgact ccacttttta ttccttattt gcccaccaaa atattataga acctaagtaa
      121 aaaaaggcgg gtggaaatcc acccgccagt taaattactg ggcttttttg agggcaccag
      181 tttttttcag ggcacttgcc acaactaggg cgatagctcc accggtgaga gccgtgatga
      241 gttggggaac ttgcatggct tgcaccattt ttggcggtgc ttggacgaaa aaccttactg
      301 cggaagaaag tacggcaaat ttcagtatgg caccgagcag gatccctacg gcatcaaagc
      361 caatgctcaa aagggctctt tcttttttgg caaaagcttt ctgtaggaaa taggaagtga
      421 agtaaaaagc tagtacatag acagcatttg ccacagcgat atatggcaca agaggtgcaa
      481 aacccatgat tcccctaatg aaagcgatta ctggagtaag gcagccgacg atcacagcgg
      541 ctaccggacc ggcaaacaca gctgagagaa tcagcatcag gttgactcca gggcctgtta
      601 tgggttgagg gaagcccccg agttggatca ctaaagttag agctaggagt aatgctgttc
      661 gcgcaattaa cttaacactg agacgcacta aaatcacctt tctttgagta ttacctggac
      721 tgaaaagaag agttcttaac cattatttct tctaggttaa ttgtgtatac tagattatac
      781 cacaaaagca agtaccaaca actaaccagt agaatgaaga aggatgaaca cagtttttat
      841 agaaaccgta taataaatgg agcaaatagc aaatttaggt cgaaggaggc atttttttca
      901 gatgattaac gctcaggttg tactgtctcc taaatgtagc aaacggctta ttgcaaaagc
      961 ggtcctggaa agcgattatt ttaaaagagc catagaaaaa ggcattgttg ttttgtcgtt
     1021 aggcacaacc aatgctttta tatatgaaga gtttacaggg aataaaatcg ccaaagaaaa
     1081 atactctgta ggcataatca ccgatgttct cacagtatcc gaatctgcag gacgcattcc
     1141 cgcattagtt cttaaaagag gcgaagttct cgacattccc tggagagaag cgctgccgga
     1201 gatggatcaa aacagtctgt ttatcaaagg tgctaatgct tacgatatta ccggcttggc
     1261 aggggtattg gtaggcggag aagcaggagg cactatcgga caggctttag gaacactagt
     1321 ggtcaacaaa gtaccgttaa tgattcccgt aggactggaa aaacgagttc cctcagtcaa
     1381 agaagcagca caactgcttt ccgaacctca cgtggaaacc aagaatatga atgtcggtat
     1441 gtttgtggtc acaaatggtt tgattatcga tgagataaga gcttttgaaa tcctgtttgg
     1501 ccttgaggcc gcattgattg ctgctggagg cttccctggg tttgagggta gcgttgtgat
     1561 caacctcaag ggcactgaag ctaaagttgt cgaagctttg gaacttgtgg agtcattaaa
     1621 gttataagga cacatctgga gaaattaata caaccatgat ttttaggggg aaaacgtatg
     1681 gataaaatcg tcgttcaagg cggttatccc ctctacgggg aagtgagcat taacggttca
     1741 aaaaacgccg cacttgcggt actggtaggt gcagctcttg gagatgaacc ctgccacgtt
     1801 gataatgtac cgcattacac agatatatac gacataatcg agattttaaa agacctcggc
     1861 gctaccgtgg aatggaccgg acgtagttct gtggttatcg acggaagcaa gctgaacaac
     1921 ccggtagctc cttacgaact agttcgtaag ttgaggggat ctttctatgt tgcaggtctg
     1981 ttactcgcca aaattgggta ctgcgaggtc ccgctgcctg gtggggatcc cttttccagc
     2041 cggcctgtca actttcattt aaaaggtttt gaggctttag gggcagaggt tacggtagag
     2101 cacggttatg taaaagccaa ggctgacaag ctggtaggca ctaccatata tgtcggtcgt
     2161 tctagcgttg gcgccacagt taatctgatg cttgcagcaa gtagggcaga aggcattaca
     2221 actttggaaa acgcagctag ggaaccagag gtcgttaacc tagccctcca gctgaactcc
     2281 atgggtgcaa agatcagagg cgcaggcacc aacatcatca ccattgaagg cgtagattac
     2341 atgaagactg caagacacga gatcattgcc gacagattgg aggcaggcac gttcttgatc
     2401 gcaacttcaa tggcaggtgg cgaagtcgtc ttgaacgacg ttgtacccga gcatttaggt
     2461 tctttgatct caaagcttag agaaagtgga gtagagatta aggatgatgg cggaattctc
     2521 acggtaaaag cccctgttgg cagaagaccg atcgcaacgg atatcgaaac aactccatat
     2581 cctggatttg ccacagacta tcagtcgatg tttgccgcat acatgaccaa agctgtagga
     2641 gtgagcattg taaaggaaac catctttgat aggtttgcct acgtcgatga gctggccaga
     2701 atgggtgcgg aaatcaaagt cgaaggagat actgcaatca tcagaggcgt tgagaggtta
     2761 accggtgcac ctgttgaggt taaagacatt cgcggaggtg ctgctctgat tatcgctggc
     2821 cttgcagcag aagggaagac catcctcgac ggtgtctacc acatcgacag aggatatgag
     2881 cacttggaag aaaaactcgc ctctctcgga gcaaaaatcg agcgcatcaa agatccaagc
     2941 taaaatcacg acatcttacc gctaaaggga tgtggctgca gtgaataaag taatcgttat
     3001 tggaagcatt catctggacc ttgtgagcag agtagaagag attcctcaac ggggcgaaac
     3061 tgtcatgggc ggagacctga attattttta cggtgggaaa ggcgccaacc aagcagtggc
     3121 cgctgcaaag caaggtgcat gtagttacat gatagggaaa gtgggcaacg accagtttgc
     3181 ccaatcgctg aaggataact taaagaacaa cggagtaaac accgattacg tttctgtaga
     3241 cccaggtagg ccaagtggaa ttgccctcat cgctgtaagc aaaagcggag aaaactccat
     3301 catcgtagct aaaggtgctt ctggagcatt gtctttcgag gaagttgttc aagcagaaaa
     3361 ccttttttcc agcggtgatt acgttctgtt tcagctggag atacctttgg agac
//
LOCUS       DAOSDA010000175         3413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_17689_length_3413_cov_2.661703, whole genome shotgun sequence.
ACCESSION   DAOSDA010000175 DAOSDA010000000
VERSION     DAOSDA010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17689_length_3413_cov_2.661703"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..245)
                     /locus_tag="PLW82_04925"
     CDS             complement(48..245)
                     /locus_tag="PLW82_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HOJ46649.1"
                     /translation="MAKNKTASDALKLEAAKRLGLDRKLLSVGWANLTSGEIGNIVRE
                     MIKMGEEALVDSQLNDIPPLY"
     gene            372..725
                     /locus_tag="PLW82_04930"
     CDS             372..725
                     /locus_tag="PLW82_04930"
                     /inference="COORDINATES: protein motif:HMM:NF022171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HOJ46650.1"
                     /translation="MNKNINPLLVKWIATFVIAVICFRFLAHGTWTTIFLLSVATVLV
                     NFLIGDVVVLPVTGSALAAACDAVLGVVAALLFAVFSPVFNPSAASLVVYGVLIAVFE
                     YVFHIYLVKTGKLVQ"
     gene            894..970
                     /locus_tag="PLW82_04935"
     tRNA            894..970
                     /locus_tag="PLW82_04935"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:928..930,aa:Val,seq:gac)
     gene            1289..3220
                     /gene="thrS"
                     /locus_tag="PLW82_04940"
     CDS             1289..3220
                     /gene="thrS"
                     /locus_tag="PLW82_04940"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOJ46651.1"
                     /translation="MIILTLPDGSKKEYQESSGITGYMVAEEISPRLAKDAIAVKLDG
                     QYFDLHRPLPHGGNFEVITSKSEEALEIIRHSASHIMAQAIKRLYPNAKFAIGPATKD
                     GFYYDIDLDKKLSDSDLELIEKEMQKIVAEDAPFSRREISQRDALAYFAAKGDQYKVE
                     ILEELDGDVSIYTQGEFEDLCRGPHLPSTGKLKAFKLLSLAGAYWRGDSKRPMLQRVY
                     GTAFADKDSLKHYLNMLEEAAKRDHRKLGKELDLFGVRDEGPGFPFFHPKGMALRNAL
                     EDFWRKIHRRAGYEEIKTPIILNEQLWRNSGHWDKYRENMYFTTIDDTNFAIKPMNCP
                     GSMLLYDRHLVSYRDLPLRYGELGIVHRHELSGTLHGLMRVRCFTQDDAHIFMRPDQI
                     EDEIIGVIDLIDYVYTDVFGFKYHVELSTRPENAFGSLEIWDLATNALKKALDRKGLS
                     YKINEGDGAFYGPKIDFHLTDSIGRTWQCGTIQLDYLMPERFQLSYIGEDGQKHQPII
                     VHRVVFGSIERFIAILTEHFAGAFPTWIAPVQVEIIPVGEAHLDAAKELKAQLDALDI
                     RAHIDSRDEKLGYRIRAAQMQKVPYMIVLGDKEVESGNVAVRSRSLGDLGSMDKAEFI
                     NNLQEEIKMLSLDSVFKKD"
BASE COUNT          908 a          786 c          857 g          862 t
ORIGIN      
        1 aaaaatttgc gctgaaacac aaaaaaggca ggatgaacct gcctttttca gtataatgga
       61 ggtatgtcgt ttagttggga atctaccagt gcctcttctc ccatcttaat catttcccgc
      121 acgatgttcc caatttcgcc agaggtcagg ttcgcccatc ccacgctgag aagcttgcgg
      181 tcgagaccta aacgcttcgc agcctctagc ttcagcgcat cagaggcagt tttgttcttg
      241 gcgatataac atcccctcta atagtatggg cgaagttctt ccgttctttc gcaaaatgat
      301 gctccttgtg ccgtatgttt tttgcagagc tgtaaaagat aggaatggag atttttgtgg
      361 aggtatggtc aatgaacaag aacatcaacc ccttactggt gaagtggatt gccacattcg
      421 taattgcggt gatttgcttt cgttttcttg cccacggaac gtggacaact atattcctac
      481 tcagtgttgc cacagttttg gttaacttcc tcatcggcga cgttgtggtt ttgccggtaa
      541 caggaagtgc ccttgcagca gcttgcgacg cggtgctggg tgttgttgcc gcactgcttt
      601 ttgccgtgtt ttctcctgtg tttaatcctt cagcggcatc tcttgtggtc tacggcgtgc
      661 tgattgcggt tttcgagtac gtcttccaca tttacttggt aaagaccggg aagctggttc
      721 agtgaagtag gttgcagctg gcgacgcttg agacttacgt gtatggaata acaagagcga
      781 aatctcgcga gaaactgcga tctatccgta caaaatcggt gcaaaaaagc ttgaatactg
      841 ttgacacgta gcggtttcgg tggtaacatg aagaagaacc tgagagaagt tatgggggcg
      901 tagctcagca ggttagagcg cttgcgtgac atgcaagagg tcatcggttc gattccgctc
      961 gctcccacca ttttcatttt aggttctttt ttaatatctt gattaatagc tgtgataagg
     1021 acgattcctg cagcacttat cagagaaaga agactgaggc aggagcctct ggctgaaagc
     1081 ttcttaacgc tgtgggtgac caccttggag ctgctctttg gaaagcttcg gcaagtaaaa
     1141 agagccggtc aaagaccgtt atcaaaaaga gaaaaatcga aggccgcagt gcttttggtt
     1201 tttaaattag ggtggaacca cggagccatt cgtcccttgg gatgaaggct ctttttttat
     1261 accgaaaatg attaaggagg tcagaatgat gattattttg actttaccag acggaagcaa
     1321 aaaagagtat caagagagtt ccggcattac aggatacatg gtagcagaag agatcagccc
     1381 acgccttgcc aaagacgcaa tagcggtaaa gcttgatgga cagtattttg atttgcatcg
     1441 gcccctacct cacggtggca acttcgaggt cattacttcc aaatcagagg aagcactgga
     1501 aatcatcagg cattcggcga gccacatcat ggcccaggct attaagcggc tctacccaaa
     1561 cgccaaattt gccataggac cggcaacaaa agatggcttt tactacgata tcgatctaga
     1621 caaaaagctc agcgacagtg atcttgaact cattgagaaa gagatgcaga agattgttgc
     1681 cgaagacgct ccgtttagcc gtagggagat ctcccaaaga gatgcgctgg cgtattttgc
     1741 cgcaaaaggc gatcagtaca aggtggagat cctcgaggag ttagacggcg acgtttctat
     1801 ctacactcaa ggggaatttg aagacctctg cagaggacct catcttccta gcaccggcaa
     1861 gctgaaagcc tttaaactgc tcagcttagc aggcgcctac tggaggggtg acagtaaaag
     1921 gcccatgctc caaagggttt acggcactgc ttttgccgac aaagatagct tgaagcacta
     1981 ccttaacatg cttgaagagg cagccaagag ggatcacagg aagctaggaa aagagcttga
     2041 tcttttcggc gttcgcgatg aaggccctgg ttttcctttc ttccatccga agggaatggc
     2101 tcttaggaat gctctggaag acttctggcg caagattcac cgtagagcag gatacgagga
     2161 gatcaagact cccatcatcc tcaatgaaca gctctggaga aactccggtc actgggataa
     2221 gtaccgtgag aacatgtact ttacaaccat tgacgatact aactttgcca tcaagcctat
     2281 gaactgcccc ggcagtatgc tcctttacga tagacatctg gtaagctaca gagacctgcc
     2341 attgcgctac ggcgagctgg gtatcgtcca tcgccacgag ctttcaggca ctctgcacgg
     2401 ccttatgaga gtacgctgct tcacccagga tgatgcccac atcttcatgc gtccagacca
     2461 gatcgaagat gagattatcg gcgtaatcga tctgatcgac tatgtctaca ccgacgtctt
     2521 cggcttcaag taccatgttg aattgagcac aagacctgaa aacgcctttg gctctttgga
     2581 gatctgggat cttgccacaa acgccctgaa aaaggctctg gaccgcaaag gcttgtctta
     2641 caagatcaat gaaggagacg gtgccttcta cggaccgaag atcgacttcc acctcacaga
     2701 cagtatcggc agaacctggc agtgcggtac tatccagttg gactacctca tgcctgagcg
     2761 cttccaactc agctacattg gcgaagacgg tcagaagcac cagccaatta tcgtgcacag
     2821 agttgttttc ggcagcattg agcggtttat cgccattttg accgagcact ttgcaggcgc
     2881 attcccgacc tggattgcac cagttcaagt ggaaatcatt ccagtgggcg aagcccatct
     2941 agatgcagca aaagaactca aagcccagct tgatgcactt gatatcaggg ctcatataga
     3001 cagcagggat gagaagctcg gctaccggat tcgtgcagct caaatgcaga aagtccccta
     3061 catgatcgtc cttggggaca aagaggttga atccgggaac gtagctgtca gatcccgttc
     3121 gttgggagat ctaggctcga tggataaggc agagttcatc aataacctgc aagaagaaat
     3181 caagatgcta agtcttgata gtgtgttcaa aaaagactaa tctaagaggg agtggatagt
     3241 ttcactccct tttttgtcca aaagcagagc aggattttcg ctccggcggg tacgtaatgt
     3301 ttgtgttgca ttcaatgccg agctagatgc gggaatgcat cacaaatgtt acaatttgtt
     3361 gtcaataccc gctgaaaatg tcagtgagta atcgagacta ttacccgatg aat
//
LOCUS       DAOSDA010000176         3392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_17841_length_3392_cov_3.098891, whole genome shotgun sequence.
ACCESSION   DAOSDA010000176 DAOSDA010000000
VERSION     DAOSDA010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17841_length_3392_cov_3.098891"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PLW82_04945"
     CDS             complement(<1..982)
                     /locus_tag="PLW82_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOJ46652.1"
                     /translation="MKTPNRKKTLKRLLAYVRPYAGYTVFGVLSLLGLALITLYLPLL
                     LGQGLIDDVLMKINENGMKTLNTLALTAVVLYVVKGLFSYAQHYCLAYLGHSVVRDIR
                     NDLLSRVLYLPLGYHQSARKGEIISKVTNDINNLQTAVGQGLGEFINQVVTGVGIIVI
                     IFVLDYRLATAACLVLPLIAFLVWVLSRRLRFLARQVQAKLADISAGLQEVLSGIRVV
                     KAYRAEKKELENLQGENQRSFAINMKSTRIMAGVLPLIEVISVIGLLIVVWFGSKEVI
                     AGRITAGTLVSFITYLGMTSGPITSLSRIISLFQQAFASAERVFELMDEPE"
     gene            complement(969..2147)
                     /locus_tag="PLW82_04950"
     CDS             complement(969..2147)
                     /locus_tag="PLW82_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46653.1"
                     /translation="MQVVITTNSPGEVTGWVIPVAKILHTLGADVDVVLTPCAFATGK
                     EADALRQTGTIRRIFSPNRYWRIALGIEKIPRDGVGCVLFLGGDIWHARRLGKLLRYP
                     VAVYSTKRADRDLDLVFVPNENVKEQVAKRVSSEKIEVVGDLMFSGLEVLGLPRKSNQ
                     IAIFPGSRAYVKSILPFFLAIAELIKERTPDLEFVISFSDFANRDLLKEALRQANPRL
                     GGTTGRVEGDAIVTEKGLSIPFYFGRSHEIMETSALALTVPGTNTAEMAFYGLPHVAI
                     LPFNIAREIPLEGLVGILSGIPRFGPWMKERLIWKKAPSLGFVALPNIMSKKALAPEL
                     KGFLNAENVAEFVLGLYQDKEKLAEMSQNLLTHFPKTDTAERIATSLCKRWEVKNEDA
                     "
     gene            complement(2125..3279)
                     /gene="lpxB"
                     /locus_tag="PLW82_04955"
     CDS             complement(2125..3279)
                     /gene="lpxB"
                     /locus_tag="PLW82_04955"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOJ46654.1"
                     /translation="MIFAGEPSGDYYGANLLKALQSLNSSIEAFGFGSQQMKKAGFDV
                     IFDPTELSVVGITEALKQIGFFRNWLKRLEVIMKERKPDCVVLIDFPGFNMRVAKLAK
                     KLKIPVVYFIPPSAWAWGKKRAYKVAETVDKVASILPMELEVYRNAGADIVSVGHPLL
                     LDIPGEIDRSKALKELGLSDSSPVLALLPGSRKQEIGTLYAPMLEACKLLKKDFPDLQ
                     VILGLSPAIKLETIEEINREVGFSPLVFQDQTHKVIAASTVCLCAGGTVTLETALLKR
                     PMVVVYKLSKITFFFVKRLVTVKYYSLPNLVLNKRVVPELIQNDCTAENMASELKPLL
                     SDPRLRVGMVDGLAKVRLRLQGEGGAEEGAYVKTAKLVLEVAKGAGSNYN"
BASE COUNT          845 a          916 c          758 g          873 t
ORIGIN      
        1 cttccggctc gtccataagt tcaaagaccc tctcggcaga agcaaacgcc tgctgaaaca
       61 aactaataat tcgcgacaaa gacgttattg gaccagaagt catgcccagg taggtgataa
      121 agctcaccag agtacctgca gtgattctgc ctgcaataac ctctttgctg ccaaaccaga
      181 ccacgatcaa aaggccgatg acgctaatta cctcgatcag cggcagcact cctgccatga
      241 tcctcgtgga tttcatgtta atggcaaaag accgttggtt ttctccctgc aggttctcca
      301 gctccttttt ttcagcgcgg taagctttga ccaccctgat accggaaaga acctcctgaa
      361 gacctgcgga gatgtccgca agctttgcct gaacctgcct ggctagaaac ctgagtctcc
      421 ggctgagaac ccaaactaaa aaagctatga ggggaagcac gaggcaggct gcagttgcca
      481 ggcggtagtc aagaacgaaa ataataacga taattcccac tccggttacc acctgattga
      541 tgaactctcc cagtccctgc cctactgcag tctgtaagtt gttaatgtcg tttgtaacct
      601 tggaaatgat ctcgcctttt cttgcacttt ggtgataacc cagcggcaga taaagcaccc
      661 tggacaaaag gtcattgcgg atgtccctaa ccacagagtg tccgaggtat gccaaacagt
      721 agtgctgggc gtagctaaac aaccctttga ccacgtacag cacaacggcc gtaagggcta
      781 gagtgttcaa ggtcttcatc ccgttttcat tgatcttcat aagtacgtca tcaataagcc
      841 cctgtccgag aaggagcgga agatacagcg taatcagggc aagacccaaa agggacagca
      901 cgccaaacac cgtgtatccc gcatagggac gcacgtacgc cagcagtcgt ttgagagttt
      961 ttttgcggtt aggcgtcttc attctttact tcccaccttt tgcatagcga agtagcgatt
     1021 ctttctgctg tatctgtttt tggaaaatga gttaaaaggt tttgcgacat ctcggcaagc
     1081 ttttctttat cttggtaaag ccccaggaca aactctgcaa cgttttctgc gttaagaaac
     1141 cccttgagtt ccggggctag agccttcttt gacatgatgt taggcagcgc aacaaaacct
     1201 agactcgggg ctttcttcca aatcagacgc tccttcatcc acggtccaaa acgaggtatg
     1261 ccgctcagaa tgcccaccag tccttccagt ggaatttctc ttgcaatgtt gaaaggcaaa
     1321 atggcaacat gaggcaggcc gtagaaagcc atttctgcgg tgtttgttcc ggggacggtc
     1381 agagcaagtg ctgacgtctc cattatctcg tgggacctgc cgaagtaaaa ggggatactg
     1441 aggccctttt cggtaactat ggcatctcct tctactctcc ctgttgtgcc tcccaagcgg
     1501 ggatttgctt gcctgagcgc ttccttcagc aggtctctgt ttgcaaagtc cgagaagcta
     1561 atgacaaact ccaagtcggg agttctctct ttgatcagtt ctgctatagc gagaaaaaat
     1621 ggcagtatac tttttacata ggcacgactt ccaggaaaga tagcgatctg gttgctcttt
     1681 ctgggaagcc ccaaaacttc cagaccggag aacatcaagt cgcctacgac ttcgatcttc
     1741 tccgagctca ctcgcttggc cacctgctct ttgacgtttt cattgggcac aaagaccaga
     1801 tccaggtccc tgtcagctct cttagtgctg taaaccgcta caggatacct caagagcttg
     1861 cccagtcttc ttgcatgcca gatatctccg cctagaaaaa gaacgcaccc tacaccatct
     1921 ctggggatct tttcgattcc cagagctata cgccaatacc tgttcggtga aaagatgcgt
     1981 ctgatagtcc cagtttgtct taaagcgtcg gcttctttcc cagtagcaaa cgcgcaggga
     2041 gtgagcacca cgtctacatc tgcgcccagc gtatgcagga ttttagcaac aggtattacc
     2101 cagcccgtga cttctccagg gctgttagtt gtaattacta cctgcacctt ttgccacctc
     2161 caagacgagc tttgccgttt ttacgtacgc tccttcttct gcgccgcctt caccttgcag
     2221 tctcaagcgc actttggcaa ggccgtccac catgcccact cttagcctgg gatcagaaag
     2281 tagcggtttt agttcgctag ccatgttttc cgcagtgcag tcgttctgta tgagttcggg
     2341 aaccaccctc ttgtttaaga ccaagtttgg tagagagtag tacttcacag taacaagacg
     2401 cttcacgaag aaaaatgtga ttttagacag cttgtacact accaccatag gccgctttaa
     2461 aagcgcagtc tcaagagtta cagtccctcc tgcacaaaga cacactgtag atgctgcgat
     2521 cactttgtgg gtctggtctt gaaaaacgag aggcgaaaaa cctacctccc tgtttatttc
     2581 ctcaatggtt tccagcttta ttgctgggga aaggcccaaa ataacctgga ggtcgggaaa
     2641 atcctttttc agcagtttgc aggcttcgag catcggcgca tataaggtac cgatctcctg
     2701 ctttctcgag cccggaagaa gagctaaaac aggagaactg tcgcttaggc ccagttcctt
     2761 tagagctttg cttctatcta tctcgccggg gatatccaaa agcagcgggt gccccacgct
     2821 gacgatgtcg gcgccggcat tgcggtagac ctctagctcc attggcagta tgcttgcgac
     2881 cttgtccacc gtctccgcta ccttgtaagc tctctttttg ccccacgccc acgctgaggg
     2941 aggtataaag tacactacag ggatcttaag ctttttggcc agcttggcca cacgcatgtt
     3001 gaaccctgga aaatcaatga gaaccacgca atcgggcttt ctctctttca tgataacttc
     3061 gagtcttttc aaccaattgc ggaaaaaccc tatctgcttt agggcttcag taatcccaac
     3121 aacgctaagt tcggtgggat cgaagatgac atcaaatccg gcttttttca tctgctgaga
     3181 accgaatcca aaagcttcaa tgctggaatt aagagactgc aacgccttta atagattggc
     3241 accgtaatag tccccagatg gttcccctgc aaaaatcata atcttcaact aaaagacctc
     3301 ccgcaaaccg caatcatctt tctgtgtatc cgtagattgc cattccgttt gcatctgcac
     3361 cctccaccac cagcacaaac tctcctttcg gt
//
LOCUS       DAOSDA010000177         3381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_17923_length_3381_cov_3.170174, whole genome shotgun sequence.
ACCESSION   DAOSDA010000177 DAOSDA010000000
VERSION     DAOSDA010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17923_length_3381_cov_3.170174"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..460)
                     /locus_tag="PLW82_04960"
     CDS             complement(65..460)
                     /locus_tag="PLW82_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOJ46655.1"
                     /translation="MDSLVKHDFQELVDKYLIRHRSILDILTKLSESAARTNRAVAKA
                     VTVCGCIRINAEKQSFPNDINYSDLRPYMENHVEGKLCESCQDVIENEIGRTIFYLTA
                     LSNALDLDLNEVIEKERKTLEALGVYNLT"
     gene            735..1220
                     /locus_tag="PLW82_04965"
     CDS             735..1220
                     /locus_tag="PLW82_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOJ46656.1"
                     /translation="MFSVGDKVVYPTHGAGVIKNIKEEDILGETKSYYVLSMLDGDLK
                     VMVPVENCHELGLRKIIDRDEVGHILDVLKESENELSSNWNRRYRNNMEKIKTGNIEE
                     VAVVARDLYSRDKEKGLSTGEKKMLEQAIRIVVSELALATETEEEVMREKILECLDTS
                     N"
     gene            1264..2232
                     /locus_tag="PLW82_04970"
     CDS             1264..2232
                     /locus_tag="PLW82_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOJ46657.1"
                     /translation="MLIRVLAFAAGLIVGGIYGWGYGNVYGLVGGMIVGACVAVLVGV
                     ITSRLKKVHGRKVLAMVLGFILGIVTGLLIGNFFQSRPLTGFLAFILGLFLADLFGNK
                     CEEMFTFTEKLPGIGRKEPTVKILDTSVIIDGRIADICATGFLEDKIIVPRFVLEELQ
                     HIADSSDILKRNRGRRGLDILNTMQKEFGMQMEIVNDEENGELEVDAKLVHLAKRIGG
                     KVITNDYNLNKVAELQGVPVLNINELANALKPVVLPGEEMNVQVIKDGKESGQGIGYL
                     DDGTMIVIDGGKKFIGEEVGVLVTSVLQTAAGRMIFAKPKALVNNM"
     gene            2341..>3381
                     /gene="ispD"
                     /locus_tag="PLW82_04975"
     CDS             2341..>3381
                     /gene="ispD"
                     /locus_tag="PLW82_04975"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOJ46658.1"
                     /translation="MKNAAIILAAGRGSRLGLGQNKMLLPFQGKPLLYYSLATFAKTK
                     LFDPIVVVVRSGEEEQVEEIVERFRADANIVLCLGGSSRVESVFFGLEALSTSGVEKV
                     LIHDGARPFVSPELILRVLDSTKPGTAAVPGVLATDSLREIDEKGMIIGTVDRSRIMQ
                     VQTPQGFMYEEIYQKHRKLWEESAAGQVTDDAQLFETQVFVEGDSRNIKITTRGDYDM
                     YNLEQPQIRVGQGFDVHRLVPGRDLILGGVKIPYEVGLLGHSDADVLTHAVMDAILGA
                     CGLGDIGEHFPDSDVAYKDACSLDLLCAVKTLLDKKGVRVLNVDATVLAEAPKIGPYK
                     DQIRDNISKCLGI"
BASE COUNT          943 a          669 c          890 g          879 t
ORIGIN      
        1 aacaaaaaaa ggctgccgca caatcagctt aatcggctaa ttgtgcaaca gccccaatca
       61 gtattcaagt aagattgtat actcctaagg cttctaacgt ttttcgctct ttttctatta
      121 cttcatttaa gtctagatct agggcattgc ttaaagctgt gaggtagaaa atcgttctac
      181 cgatctcatt ttctataaca tcttgacagg actcgcagag tttgccttca acgtggtttt
      241 ccatgtatgg tctaagatcg ctgtagttta tgtcgttggg gaaggactgc ttctcagcat
      301 tgattctgat gcacccgcaa actgtaactg ctttagcaac agctctgttt gtcctggcgg
      361 cagattctga aagctttgtc aatatatcta aaattgatcg gtgccggata aggtatttgt
      421 cgacgagttc ttgaaagtcg tgcttgacca gactgtccat agagtaccct ccttcggcta
      481 catctccctt aaattataaa aatacagctt tgacaatgtc aaggtgtatt tggcttggtc
      541 tggctccgtc tagggcgcca ggcgcgttgg ttggagggca cagaccgtgc agcattgaaa
      601 gtcttgaaaa ataatgggca ttatctgttg acatggcctg taagtttgtg ttaaaattat
      661 taaatttctt gactaagttg tcaaaaaggc ttataataac agataataca gagcctttaa
      721 gaggaggtgt aacggtgttt tctgttggag ataaagttgt ataccccaca catggagcgg
      781 gtgttattaa gaacattaaa gaagaagaca tcttaggcga aacgaaatca tattacgtac
      841 tttctatgtt ggatggagat ctcaaagtga tggtaccagt cgaaaactgc catgaactcg
      901 gtcttcgcaa aatcatcgac agagacgagg taggccacat tcttgacgtg ttgaaagagt
      961 cagagaatga actcagttcc aattggaatc gccgttatcg caacaacatg gaaaagatca
     1021 agaccggcaa catcgaagaa gtagcggttg tggctcgcga tctttattca agagataaag
     1081 aaaaaggtct ctctacaggg gaaaagaaga tgctggagca ggctatccgc atcgtcgttt
     1141 cggagcttgc tttagccacc gaaacggaag aagaggtgat gagagaaaaa atactagaat
     1201 gcctcgatac tagcaactaa tctaactaac caaaaacggt aaggcaaaaa aggcggtgaa
     1261 agggtgttaa tacgggtatt agcttttgcc gcaggcctga ttgttggcgg aatctatggg
     1321 tggggatacg gcaacgttta cgggcttgtg ggcggtatga ttgtaggtgc ttgtgtagca
     1381 gttttggtag gggtaattac cagccggctt aagaaagttc acggccgaaa agtcttggcg
     1441 atggtcctag gatttatcct aggcattgtc actggtcttt taattggtaa cttcttccaa
     1501 tctaggccac ttacggggtt tttggcattt attctaggtc ttttcttggc agatttgttt
     1561 ggcaataaat gtgaagagat gtttaccttc acagaaaagc ttccgggaat tgggagaaaa
     1621 gaacctacgg tcaagattct cgacaccagc gtgatcatcg acggccgaat cgccgacatc
     1681 tgtgctactg ggtttttaga ggacaaaatc atcgtaccgc ggtttgtcct cgaagagctg
     1741 cagcacatag ccgattcgtc ggatattctt aaacgtaata ggggtcgaag aggccttgac
     1801 atcttgaaca cgatgcagaa agagttcggc atgcagatgg agatcgttaa cgacgaagaa
     1861 aacggagaat tggaagtcga tgcaaagctg gtgcatcttg ccaagcgcat cggcggaaag
     1921 gtcatcacca atgactacaa cttaaataaa gtagctgaac tgcagggggt tcctgtacta
     1981 aacatcaacg aactggcaaa tgcactaaaa cctgtagtgc ttcccggcga agagatgaat
     2041 gttcaggtga ttaaagacgg caaagagtcg ggacagggta ttggctacct tgatgacggt
     2101 actatgatcg tcatcgacgg cggtaagaag tttatcggcg aagaggttgg cgttttggtc
     2161 accagcgtgt tgcaaactgc cgcaggaagg atgatctttg ccaagcccaa agcattggtg
     2221 aataacatgt gaaaggcacc cgccattttg gcgggtgttt attgatacct gcggttctgg
     2281 acaaatgtcc ctgctgcagg tatgctgaat ctatagataa tggatgcggg aggagcggct
     2341 atgaaaaacg cagcaattat tctggcagct ggcagaggca gccgattggg actaggtcaa
     2401 aacaagatgc tcctgccatt ccaaggtaaa ccgcttctgt actactcgtt agcaacattt
     2461 gccaagacca agctgttcga tcctattgtc gtggttgtaa ggagcggcga agaggaacag
     2521 gttgaggaga tagtggaaag gttcagagca gatgctaaca tcgtactctg tttggggggt
     2581 agctctcgtg tagaatcggt gtttttcgga cttgaggcgc tatccacctc gggtgtggag
     2641 aaggtcttga ttcatgatgg cgccaggccg tttgtcagcc cagagttgat tttaagggta
     2701 ttagatagca caaagcccgg cacggcagct gttcctggag tccttgccac agactctttg
     2761 cgagagatcg acgaaaaagg tatgattatt ggtactgtag atcgaagccg catcatgcag
     2821 gttcaaaccc cccaaggatt tatgtatgag gaaatctacc aaaagcaccg caagttgtgg
     2881 gaagaatctg ccgcaggtca ggttaccgac gatgcgcagc tgtttgaaac gcaggtgttt
     2941 gtggaaggag atagccgaaa cataaaaatc acaactaggg gagactacga tatgtacaat
     3001 ttggaacaac cccaaatccg ggtgggtcag gggtttgatg tccaccgcct agtacctggg
     3061 agagatttga tcctaggggg cgttaaaata ccatatgaag tggggctttt aggtcattcc
     3121 gatgcagacg tgctgactca tgcagttatg gatgcgattc ttggggcctg cggattggga
     3181 gatatcggcg agcacttccc tgattcagat gttgcctaca aagatgcctg cagtttggac
     3241 ctgctctgtg cagtgaaaac cctattagac aagaaaggcg tcagagttct taacgttgat
     3301 gccacagttt tagctgaagc accaaagatc gggccttaca aagaccagat acgggataac
     3361 atcagcaaat gtctcggtat a
//
LOCUS       DAOSDA010000178         3370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_18017_length_3370_cov_2.430769, whole genome shotgun sequence.
ACCESSION   DAOSDA010000178 DAOSDA010000000
VERSION     DAOSDA010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18017_length_3370_cov_2.430769"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    517..753
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            796..2565
                     /gene="ilvB"
                     /locus_tag="PLW82_04980"
     CDS             796..2565
                     /gene="ilvB"
                     /locus_tag="PLW82_04980"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOJ46659.1"
                     /translation="MAKTGAEILVDTLVELGVEYVFGYPGGAVLPIYDALYSSSLKHI
                     LTRHEQGAAHAADGYARTSGKVGVVIATSGPGATNLVTGLANAYMDSVPLVAITGQVQ
                     SGLIGKDSFQEADVLGITMPITKHNYLVKDENRIESVLREAFYIASTGRPGPVLVDIT
                     KDALMNYVSRKKSKNGLDLPGYRVLKEGHPRQIALARKLIKEAQKPLLYVGGGAQDAS
                     EILIQFAENLDIPVTTTLMGIGAFPGDHELSLGMLGMHGSPWANLAVQNCDLLIAVGA
                     RFDDRVTGSLSKFAEKAKVIHIDIDAAEIGKNVAAYVPIVGDAKTVLAQLLDDTKLCH
                     REWIQEIRNFELPMQLPKEPKITKCTDCTAPNMKNSDVPPPCRKKDSVDPKVVLEMVS
                     DLLQDDLIVATDVGQNQMWAAQYVRVKKPRSFITSGGLGTMGYGLPAAIGAKLGRPDR
                     SVVLVTGDGSFQMNIQELATIASYGIPVKIVLLNNRVLGMVRQWQTIFYESRYSQTCT
                     GSVPDFCVLASAYGIPAVRIKNQDQLGLLQEALTSEGPYLVEVSVDPDESVWPMVAPG
                     AALCEMFHAAKAPTPSWQRRAKS"
     gene            2562..3056
                     /gene="ilvN"
                     /locus_tag="PLW82_04985"
     CDS             2562..3056
                     /gene="ilvN"
                     /locus_tag="PLW82_04985"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOJ46660.1"
                     /translation="MNENRREKVTLAVLVRNEPGVLSRVAGLFTRRGYNIHSLAVGPT
                     EKKEISRITIVIEEEPERLEQVEKQLHKLIDVLKIINLTNVQRVERELCLIQVNGEKR
                     SDILQIVDIFRAKIVDVSPKEVMVEVTGDTEKIDAIIQMLAGYGISEIVRTGKIALAR
                     STQS"
     gene            3071..>3370
                     /locus_tag="PLW82_04990"
     CDS             3071..>3370
                     /locus_tag="PLW82_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOJ46661.1"
                     /translation="MAKLYYNEDADLGVLNGKKVAILGYGSQGHAHALNLKDSGVDVV
                     VAQRPGSKNYDLAVSHGFTPVSVKEAAEIADVIVVLLPDEKQKAVYEQDIAPNLKP"
BASE COUNT          915 a          818 c          893 g          744 t
ORIGIN      
        1 gcaaaaacac aaaactgatc ttggatagca ctaggatgtt caaagaaacc agtaggatgc
       61 agatacctat cttaaaccac ttggagttct gcatggtctc acacctttct gctgtggatc
      121 atgtccatat cgacgttttc gcctctatgt tagcatacta actgctcttc ctaaacaaaa
      181 aggtccaaaa gagatttaag gaagccacga tcggtaagcg gggagctgtg caatcttcaa
      241 gaagtaggta ggatttaagc actatttcgc ggtgaacata agcaacctac cgggctttaa
      301 actgattggg acgagcgtgt cgggtttttt tacggaaaaa tgcttgcttt ttccagttaa
      361 taccgtataa tagacagcaa aaacattttt agcacttcag aatatcagta tgttaaaatg
      421 ctaacatatc tccgaaaaaa actacgaaat tggtattgac acagggcatg ttggcacgta
      481 tactaaaaaa caaaaccttt ttcagtacga atatctgcaa agacgatgaa agagagtagt
      541 actgtcttgc tgccttcaaa gagagtcgcc ggttggtgaa agagcgacaa ggagcaacca
      601 ggaagcatcg ctctggagtc gccaatgcga aaggaagccc aaagagctta ggcataactt
      661 tgtgcagctt ctcagtagtg ttggccgggt cagcccgtaa cagctctgaa gtgataaatt
      721 agggtggtac cgcgaacagt cctcgcccct tgctggggtg aggttttttt taaaattttt
      781 cgggaggtga cccgtttggc gaaaacagga gcagagattt tagtagatac tttagtggag
      841 ctaggtgtag agtacgtttt tggctatcca ggcggagctg ttctgccgat ttacgacgcc
      901 ctctacagct ccagcctgaa gcacattctc accaggcacg aacaaggcgc agcacacgca
      961 gccgacggtt atgctagaac atcgggcaaa gtgggtgtgg ttattgccac ctccggtccg
     1021 ggagccacaa acttggtgac gggccttgcc aatgcctata tggattcagt gccccttgtt
     1081 gccattacag gtcaggtcca gtcaggtctg atcggaaagg actcttttca agaagctgac
     1141 gttcttggca taacgatgcc tataactaaa cacaactact tggtgaagga cgaaaaccgt
     1201 attgaatcgg tgttgcggga agcattttac attgccagca ccggtcgtcc gggacctgtc
     1261 cttgtggata tcaccaaaga cgctctgatg aactacgtca gcaggaagaa aagcaaaaac
     1321 ggcttggatc tgccgggata ccgggtgctc aaagaaggcc atcccaggca gatcgccctc
     1381 gcccgcaagc tgattaagga ggctcaaaaa cccctactgt atgtaggggg aggggctcag
     1441 gatgcaagcg agatcctaat tcagttcgca gaaaacctcg atataccggt aaccaccact
     1501 ctgatgggca tcggcgcgtt cccaggagat cacgagctga gcctgggcat gctgggcatg
     1561 cacggaagtc catgggcaaa ccttgcagtg cagaactgcg atctgttaat tgcagttggt
     1621 gcaaggtttg atgacagggt taccggctct ctcagcaagt ttgccgagaa agccaaagtg
     1681 atccacattg atatcgatgc cgctgaaatc ggcaagaacg ttgcagctta tgtacccatc
     1741 gtgggcgatg caaagacggt cctagcccag ctcttagacg acactaagct ctgccacagg
     1801 gaatggatcc aggagatcag aaacttcgag ctgccaatgc agctgccaaa agaacccaaa
     1861 ataacaaagt gtacagactg cacagccccg aatatgaaaa actcagacgt tccgccgccg
     1921 tgcagaaaaa aggacagcgt cgatccgaag gtagtcttgg agatggtgtc cgatcttcta
     1981 caggatgatc tgattgtcgc caccgacgtt gggcaaaacc agatgtgggc agcccagtat
     2041 gtaagggtga aaaaaccccg cagctttatc acttcaggtg gccttggcac catgggatac
     2101 ggtctacctg cagccatcgg agcaaagctc ggaagacccg ataggagtgt ggttcttgtc
     2161 accggcgatg gctccttcca gatgaacatc caggagcttg cgaccatcgc cagctacggc
     2221 atcccggtta agatcgtcct cctcaacaac cgggtattgg gcatggtgcg gcagtggcag
     2281 accattttct acgaaagccg ctactcccag acttgcaccg ggtcggtccc ggacttctgc
     2341 gttcttgcca gtgcatacgg gattcccgct gtaaggatca aaaaccagga tcagctcggt
     2401 ctgctgcagg aagcccttac cagcgaagga ccctatctgg ttgaggtttc ggtagaccca
     2461 gacgagtcgg tttggccgat ggtagcgcca ggagcggctc tctgcgagat gttccatgct
     2521 gccaaagctc ccacaccttc ttggcaaagg agggcaaagt cgtgaacgaa aataggcggg
     2581 aaaaagtcac tttagcggtt ttggtgcgca acgaaccagg tgtgctctcg cgggtagcag
     2641 ggctctttac caggcggggc tacaacatcc actcccttgc agtaggtccc acagaaaaga
     2701 aggagatctc ccgcatcacc atcgtcatcg aagaggaacc ggagcgcttg gaacaggttg
     2761 aaaaacagct ccacaagctc atcgacgtgc tgaagatcat caacctcacc aacgtgcagc
     2821 gggtggaaag ggaactctgc ttgatccagg ttaacggcga gaagcgttcg gatatcctcc
     2881 agatcgtcga catcttcaga gccaaaatcg tcgatgtgag ccctaaagag gtgatggtgg
     2941 aggtcaccgg agatacggag aagatcgatg ccataattca gatgcttgca ggttatggca
     3001 tctcggagat agttcgtaca ggtaaaatcg ctctggcacg gagcactcaa tcataaggag
     3061 gtttttgaaa atggcaaagc tttactataa cgaagatgcg gatctcggcg ttttgaacgg
     3121 taagaaagta gcgattttgg gatacggctc tcaaggtcac gcccacgctc tcaatctcaa
     3181 ggacagcggc gtagacgttg tggttgcaca gcgtcccggt tccaagaact acgatctggc
     3241 agtctcccac ggcttcactc ctgtgagcgt gaaagaagct gcagagatcg ccgacgtaat
     3301 cgttgttctc cttccagacg agaagcagaa agccgtatat gaacaggaca ttgcaccgaa
     3361 ccttaagcca
//
LOCUS       DAOSDA010000179         3369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_18023_length_3369_cov_2.742305, whole genome shotgun sequence.
ACCESSION   DAOSDA010000179 DAOSDA010000000
VERSION     DAOSDA010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18023_length_3369_cov_2.742305"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PLW82_04995"
     CDS             <1..810
                     /locus_tag="PLW82_04995"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HOJ46662.1"
                     /translation="GEIANREARRGGLFLGAALGSLYYLAFLLLTKGGFSLPPWLLIV
                     SSLLMPAFMVLLAFNKRDLKAVLPMVYLLALIAGGIGYWLQSMNVARVWVLLGPHVAV
                     LLIAEIGWGLLHERFWESFGQIALVVEINGQKQALRALIDSGNELVEPVSGKRVVIVE
                     KDVMKNLVPAEIMQSIEQMTPIGGDPDSAEGWNKRLVLIPFKSVGRDSGVLVGLRPDS
                     VYLKHKGKQVRLPGVIIGLETGSLSQRGEYQALIHPALLLANSGGEQREMA"
     gene            827..1537
                     /gene="sigE"
                     /locus_tag="PLW82_05000"
     CDS             827..1537
                     /gene="sigE"
                     /locus_tag="PLW82_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HOJ46663.1"
                     /translation="MKGLSLLDVKLFLSQFLKRRRHVFYVGSSEVLPPPLLPEEERVL
                     LKKLQAGEASVKSTLIERNLRLVVYIARKFENTGVGIEDLVSIGTIGLIKAVNTFDIG
                     KNIKLATYASRCIENEILMFLRRHQRTKEVYLDDPLNTDWDGNELSWLDVLPASEGDG
                     VYQVLEEETDKNLLKAALLKLEPREQEIVTLRFGIGIDRELTQKEVADKMGISQSYIS
                     RLEKRIMKKLRRELKKFE"
     gene            1649..2443
                     /gene="sigG"
                     /locus_tag="PLW82_05005"
     CDS             1649..2443
                     /gene="sigG"
                     /locus_tag="PLW82_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HOJ46664.1"
                     /translation="MINKVEICGVNTAELPVIKNEKMKMLLAKVKEGDQAAREELIQG
                     NLRLVLSVIQRFNNRGENVDDLFQVGCIGLMKAIDNFDLSQNVRFSTYAVPMIIGEIR
                     RYLRDNNPIRVSRSLRDIAYKALQIRDKLAGEKGTEPSLSEIAENLGVDREEIVYALD
                     AIQEPISLFEPIYHDGGDPIFVMDQIDDEKAKDSIWIEEISLREAIDRLDERERKILS
                     MRFFEGRTQMEVAEEIGISQAQVSRLEKAALFDLKLHYSEGEATNQ"
     gene            2440..3054
                     /locus_tag="PLW82_05010"
     CDS             2440..3054
                     /locus_tag="PLW82_05010"
                     /inference="COORDINATES: protein motif:HMM:NF021093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HOJ46665.1"
                     /translation="MRKRLRREKLERAALVFLAVCLFPLFLGFADGSQEFLRLHVLAN
                     SDSFEDQMLKLQARDAVLAEMNVLLSGQKDLEKVVEIIKGSEDELVQKASEAIGYKYP
                     VKIELGREDYPERSYGNLILPSGEYLSLKVIIGEGSGQNWWCVLFPLLCSIEAGENAI
                     EVVDGEGGPPVKFRFKLAQNEMKKPPSLDAVLAFLQQFRLWPWS"
BASE COUNT          914 a          757 c          905 g          793 t
ORIGIN      
        1 ggagagatag ccaatcgaga agcgcgtcga ggtgggcttt ttttaggcgc cgctttaggc
       61 agtctgtact acttggcatt tttgctttta accaaaggtg gtttttctct gccgccgtgg
      121 cttctgatcg tctcgtcgct tttgatgcca gcatttatgg tgctccttgc tttcaacaaa
      181 agagacctaa aagctgtttt gcctatggtc taccttctag ctctgatcgc aggaggcatc
      241 ggctactggc tgcagtctat gaatgtagcg cgggtgtggg tgcttttagg cccacacgtg
      301 gcagtgctcc tcatcgccga gatcggttgg ggactccttc acgaacgctt ttgggagagt
      361 ttcggtcaga ttgctttggt tgtggagatt aatgggcaga agcaggcact cagagctctc
      421 atcgacagcg gtaacgaact tgtggagccg gttagtggca aacgagtggt aatcgttgag
      481 aaagatgtga tgaagaacct agttcctgca gagatcatgc agtctataga gcagatgacg
      541 ccaattggcg gtgatccgga cagcgcagaa ggttggaaca agcgcttggt ccttattccg
      601 tttaaatcgg taggtcggga cagcggtgtt ttggtgggac tcaggcctga ttctgtttac
      661 ctgaagcata aaggcaaaca agttaggctt ccaggagtga tcatcggttt ggaaacgggc
      721 tccctcagcc aacgaggcga gtatcaagcg ctgattcatc cggcacttct tcttgcaaac
      781 tctggaggtg agcagcgtga aatggcttaa aaccctcttt gggaagttga aaggcctctc
      841 acttctagat gttaagctct ttctcagcca gttcctcaaa agacgccgcc acgtctttta
      901 cgtcggctct agcgaggtac tgccgccgcc ccttcttcca gaagaagaac gggtgctgct
      961 gaaaaagctc caagcaggag aagcgtcggt taagagcact cttatcgaaa gaaacctgcg
     1021 ccttgtggta tatattgcca ggaagttcga aaacaccggt gtaggcattg aagatctggt
     1081 gtctatcggc accatcggat tgataaaggc cgtaaacacc tttgacatcg gcaagaacat
     1141 taagctggca acctatgcct ctagatgtat cgaaaacgaa attttgatgt ttctcaggag
     1201 gcaccaaagg acaaaagagg tctatttaga cgacccgcta aacacagact gggacggaaa
     1261 tgagctctcc tggcttgacg ttcttcccgc ctctgaaggc gacggcgtgt accaagttct
     1321 ggaggaagaa accgacaaaa atctgctgaa agctgccctc ctgaagctcg aacccagaga
     1381 gcaggagatc gtgacactcc gctttggcat cggcatcgac cgcgaactca ctcaaaaaga
     1441 agtggcagac aaaatgggta tctcccagtc gtatatttcc cggctggaaa agcggattat
     1501 gaagaaactg cgtcgagaac tgaaaaagtt cgaataaaaa accaaggccg actctttagg
     1561 gccttggttt tttttgtccc cttcgggtat atctttagcc ttttgcaccc atactttcta
     1621 tgagatggcc cgtagggagg aaaagcagat gattaataaa gttgagatat gcggagtaaa
     1681 cacggccgaa ctgccggtca taaaaaacga gaaaatgaaa atgctcctgg ccaaagtcaa
     1741 agaaggagat caagctgcaa gagaagagct gatccagggg aacctcaggc tggtcctcag
     1801 cgtcattcag cgcttcaaca accgcggcga aaacgtcgat gatctctttc aggtgggctg
     1861 tatcggttta atgaaggcca tagacaactt cgatctctcg cagaacgtgc gcttctcaac
     1921 gtatgcagtg ccaatgatca tcggtgaaat cagacgctac ctcagagaca acaaccccat
     1981 tagagtcagc cgttcgctgc gggatattgc gtataaagct ctgcagatca gggacaagct
     2041 ggcaggagaa aaaggtacgg aaccttcgtt aagcgagatc gccgaaaacc tcggcgtgga
     2101 tagagaggaa atcgtctacg ccctcgatgc cattcaggaa cccatttctc tgtttgaacc
     2161 gatctaccac gatggtggag atcctatctt cgtaatggac caaatcgacg acgaaaaagc
     2221 taaagacagc atatggattg aagagatctc tttgagggaa gctatagata gactagatga
     2281 aagagagcga aagatcttaa gcatgcgctt ttttgaaggt aggacccaaa tggaagttgc
     2341 agaagagatc ggcatctccc aagcccaggt ctcccgtctc gaaaaagccg cacttttcga
     2401 cctcaagctg cactactcag agggggaggc gaccaaccaa tgagaaagcg cctcaggaga
     2461 gagaaactgg aaagggcggc actggtcttt ttggcagtat gtctctttcc gttgtttttg
     2521 ggttttgccg atggctcgca ggagttcttg aggttgcacg ttttggccaa ctcagacagc
     2581 ttcgaagacc aaatgctgaa gctgcaggcg cgagatgcgg tcttggctga gatgaacgtg
     2641 ctgctttcag gacaaaagga tctggagaag gtagtggaga tcatcaaggg tagcgaagat
     2701 gagttagtgc agaaagcttc tgaagccata ggatacaagt atccggtgaa gatagagttg
     2761 ggtagggaag actacccgga aagaagctac ggaaacctca tccttcccag tggagagtac
     2821 ctctcgctta aggtgataat cggcgaagga agcgggcaga actggtggtg cgttctgttt
     2881 ccgctgctct gctcgataga agccggagaa aacgccatcg aagttgtaga tggcgaggga
     2941 ggaccgccag ttaagttccg tttcaaactg gctcaaaacg aaatgaaaaa accaccttcc
     3001 ctagatgcag tgcttgcatt cctacagcag ttccgcctct ggccttggtc ttgaggcggc
     3061 tttttttttg ccccaaaaac ctttttccag ctggaagtcc atccatcctt agaggtgcgg
     3121 agaatctgga ttaagcaaaa tcaaggagat aggcatttgg taaaccgctt ttaggctttg
     3181 ttagagcaaa atctcaccaa cctgtgccat aatcccgact aacgcataac ctaaagagga
     3241 ggtgtaagcg gtgggcacta ggttaaacga cttaagacaa aaagagattg tggatactgc
     3301 aacaggaatg aagcttggcg agattatcga tttggaactt gatatgcata caggtcaaat
     3361 cacggcgct
//
LOCUS       DAOSDA010000180         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_18030_length_3368_cov_2.793842, whole genome shotgun sequence.
ACCESSION   DAOSDA010000180 DAOSDA010000000
VERSION     DAOSDA010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18030_length_3368_cov_2.793842"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLW82_05015"
     CDS             complement(<1..657)
                     /locus_tag="PLW82_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOJ46666.1"
                     /translation="MSLRYDILILRYGELALKGRNRATFENLLIKQTRRVLKDLEGWK
                     LTRPSRRWILDLGGNDPEEVVKRLQKVFGYVSFSPAVVLKEPTIEDIKETARLIAQNI
                     DPGVETFKVNTRRVDKKFPFESLEVSKELGGVVLKELDGRLKVDVHNPQWVLNVEIRP
                     EGTYLFSQSYPGQGGLPYGSGSKALLLLSGGIDSPVAGYLIMKRGAVVEGLHFHSFPF
                     T"
     gene            complement(670..1791)
                     /locus_tag="PLW82_05020"
     CDS             complement(670..1791)
                     /locus_tag="PLW82_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOJ46667.1"
                     /translation="MIYLDNSSTTKPYPSVVEACRWALEENFGNPSSLHRLGFQAEKA
                     VKEARENVAALINVSPEEIVFTSGGTEGNYLAIRSVVEGSKDKHIITSVVEHPSVMAV
                     FEALEAAGWEVDYIPVDKTGHVDVDVLKESLRKDTVLVSIMAVNNELGTNQPISEIGK
                     LLKDHPKTYFHVDAVQAVGKVAVDMTRVDLLTMSAHKIHGPKGIGALYVRRGRKFESI
                     MPGGGQERGFRGGTENVPAIVGFGEAAKIALKKMPEIGILKAKRDELAAEIKRLVPDC
                     TINSPAESVLILNVSFPNIKAEVLLHALEEEGIYVSTGSACSSRKKAHPVLVAIGLDE
                     KLHDGTIRFSLGWGNEDIDPGYIAKVTAEKVREIQLLLK"
     gene            complement(1921..2733)
                     /locus_tag="PLW82_05025"
     CDS             complement(1921..2733)
                     /locus_tag="PLW82_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46668.1"
                     /translation="MRLQTKLLVLTLVFCLSLTALAGSFNVKVSRGYETLQLESGAGY
                     TDKTSTLLDTAAIDWVIAGKLGVFAALEKGSTVVVQVDTDHSYPIDGLKGHYSRQMLG
                     AAYYVGKGDFKVGVGIGLDLDREQFSWTGNNDNTVNRQWQWDRNSIGVAGLARLVYDG
                     PSAGFTAGISYTPFFKSDETLEAEVEDTWESWETAGLDAVLLKVNGEITVSLARYMGL
                     VVRVSYRDYRSPETNVPSFYKNSNGDQKEMEVQANKVAEKGAYIYAGIKVSF"
     gene            complement(2747..>3368)
                     /locus_tag="PLW82_05030"
     CDS             complement(2747..>3368)
                     /locus_tag="PLW82_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46669.1"
                     /translation="TEDLEYEWVLGYLSYSRGSATGKPSADLFAAARLGFDYQLESVR
                     LSFSPGVLLSDLGESLGVGVPFRLERVGHVLTGEVALYALFGSSVLKEGLFPAAVVRY
                     HPNGSPIEVEVAYIAQNFLPYLGNFANFGGTLDWSTGSSGIQLKLVLQNYICSLKFEQ
                     TPPDSRGVFFDIRRHLPQPFSPLSVGLGVKDRKGFISLKTGLTFSF"
BASE COUNT          798 a          935 c          753 g          882 t
ORIGIN      
        1 ggtgaagggg aagctgtgga agtgcagccc ctctacaact gctccgcgct tcatgatcag
       61 atagcctgca acaggactgt ctatccctcc tgaaaggagc aacagggctt tgctgccact
      121 gccgtaggga agccctccct gcccgggata cgattgactg aaaagatagg ttccctccgg
      181 tctgatctct acatttagca cccattgggg attgtggacg tcgactttaa gcctgccatc
      241 tagctccttg aggacgactc ctcctagttc cttcgagact tctaggctct caaaggggaa
      301 cttcttgtcc actctcctgg tgttaacctt gaacgtctcc acaccaggat ctatattttg
      361 agcgattagt ctggccgtct ctttaatatc ttctatagtg ggctctttga gaaccaccgc
      421 cggagaaaag gaaacgtagc cgaaaacctt ctgcagcctt tttaccactt cctccggatc
      481 gttacctcca agatcaagta tccagcgcct tgaaggtctt gtgagcttcc agccctcaag
      541 atccttaaga acgcgtctgg tctgtttaat caacaggttt tcaaacgtag ctcgatttct
      601 gcctttaaga gcgagctctc cgtatctgag aatcaaaata tcataacgca aactcatcta
      661 tgtgtcctcc tacttcaaga gaagctggat ctctcgaacc ttttctgccg tgactttggc
      721 aatgtaaccg gggtcaatgt cctcgttgcc ccaacctaac gaaaatctga tggttccatc
      781 gtgcagtttt tcatctagac cgatcgcaac aagcactggg tgtgctttct tcctcgagga
      841 gcacgccgaa cctgtggata cgtaaatacc ttcttcctct aaagcgtgga gtagcacctc
      901 ggctttgatg ttgggaaagc tcacgttaag aatcaaaacg ctttccgccg ggctgttgat
      961 ggtgcagtcg ggaacaagtc tcttgatttc cgctgcaagt tcgtcgcgtt tggctttgag
     1021 aatcccaatt tctggcattt tcttgagagc gatcttagcc gcttcgccaa aaccgactat
     1081 ggcaggaacg ttttcggttc cgcctctaaa gcctctctcc tgccctccac caggcataat
     1141 cgattcaaac ttccgccctc ttctaacgta aagggcgcct atgcccttgg gtccgtgaat
     1201 cttgtgggcg ctcatggtca aaaggtccac cctggtcata tccacagcga ctttacccac
     1261 tgcttgtaca gcatcaacgt ggaagtaggt ttttggatgg tccttgagca gctttccgat
     1321 ctcggagata ggttgattgg tccccagttc gttgttgacc gccatgatcg aaaccagcac
     1381 tgtgtccttt ctgagactct ctttcagaac atcgacatcc acgtggccgg ttttatcaac
     1441 agggatatag tccacttccc agcctgctgc ttctagggct tcaaacactg ccatcaccga
     1501 gggatgttcc accaccgagg tgatgatgtg tttgtctttt gacccctcga ctacagagcg
     1561 gatcgccaga tagttacctt ccgttcctcc agaggtaaaa acaatctcct caggagatac
     1621 atttatcaga gcagcaacgt tttctctagc ctcttttact gctttttcag cctgaaaacc
     1681 aagtctgtgc aggctagaag ggttgccgaa gttctcttca agagcccacc tgcaggcttc
     1741 tactactgat gggtagggct ttgttgtcga gctgttgtcc aaatagatca tcaaggtccc
     1801 tccaacttgt acttccgctt ctaatctatc ataccacaga ttgctctatt gttatcacaa
     1861 cagattaggg ggtacacaaa gcaatttggc tgcccgaggg cagccaaatt gcgcgtcttt
     1921 ttagaagctc accttgatgc ctgcgtagat gtaggcacct ttttctgcta ccttgttggc
     1981 ttgaacctcc atctccttct gatcgccgtt actgttttta tagaagcttg gcacattggt
     2041 ctcgggactc cggtagtctc tgtagctgac ccttaccacg agacccatgt agcgggcaag
     2101 ggaaacggtg atctctccgt ttactttgag gagtactgcg tcgagtccgg cagtttccca
     2161 cgactcccaa gtgtcctcta cttcagcttc taaagtctcg tcagacttga aaaacggcgt
     2221 gtagctgatc ccggcggtaa aacctgcgct tggaccgtcg tagactagcc tggccagccc
     2281 agcaacaccg atagagtttc ggtcccactg ccactgcctg ttaaccgtat tgtcgttgtt
     2341 gccagtccag gaaaactgct ccctatccaa gtccaaacca attcccaccc cgactttaaa
     2401 gtcgcccttc ccaacgtagt aagcagcgcc cagcatctga cggctataat gccctttcag
     2461 accatctatg ggataggaat ggtcggtgtc aacttggaca acaacggtgg aacccttctc
     2521 caaagcggca aaaacaccta atttgccggc gattacccaa tctatggcag ctgtatccag
     2581 aagagtactg gtcttgtctg tgtacccagc accgctttct agctgcagcg tttcataacc
     2641 cctgctaact ttgacattga agcttcctgc aagagcggtc aggctgaggc agaaaaccaa
     2701 tgtcaaaacc agaagcttgg tctgcaatct caaataaacc acctacctaa aaactgaatg
     2761 ttaagcctgt tttcagactg atgaatccct tcctgtcctt tacaccaaga ccaacactta
     2821 gaggcgaaaa tggctgcggt aggtgcctcc gtatatcaaa gaaaactcct cgggaatctg
     2881 gtggagtttg ttcaaacttc aaggaacaga tgtagttctg gagtaccagc ttaagctgta
     2941 tccccgacga ccctgtactc cagtccaacg ttccaccgaa gtttgcaaag ttgcccagat
     3001 aaggcaggaa gttctgggcg atgtacgcaa cctctacttc tatgggagac ccgttaggat
     3061 ggtaccttac aaccgctgca ggaaaaagcc cttctttcag tactgaagac ccgaacaggg
     3121 cgtacagggc aacttcgccc gttagaacgt gtcctactct ttccaagcgg aaaggcactc
     3181 ccactcccaa ggactctcct aagtcgctga gaagcacccc ggggctaaac gataacctca
     3241 cagattctag ttgatagtcg aatcctaaac gtgccgctgc aaacaagtcc gcgctgggtt
     3301 taccagttgc gctcccacga gagtagctca agtaaccgag cacccactca tactcgagat
     3361 cctcagtt
//
LOCUS       DAOSDA010000181         3361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_18092_length_3361_cov_2.957955, whole genome shotgun sequence.
ACCESSION   DAOSDA010000181 DAOSDA010000000
VERSION     DAOSDA010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18092_length_3361_cov_2.957955"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLW82_05035"
     CDS             complement(<1..501)
                     /locus_tag="PLW82_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOJ46670.1"
                     /translation="MYKITKTDLEYLEQIVGKEFVFAGDEVNPDYTHDEIYGMEDFPG
                     CIVFPTSAEEISAIMKYASSNKIPVTVRGSGTGLCGGCVPIYGGILLVTTRMNRVLEI
                     DEDNLTATVEPGVILLSFQEELERFGLFYAPDPGEKSATIGGNVMTNAGGMRAVKYGV
                     TRDHVLG"
     gene            complement(506..1708)
                     /locus_tag="PLW82_05040"
     CDS             complement(506..1708)
                     /locus_tag="PLW82_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOJ46671.1"
                     /translation="MSYLGVNHDKCILCGVCVDSCPFGALKITGKLEVLDSCRLCKVC
                     IKVCPEKALSVAEGSLTKSDLSSYRNVLVFAEQRDGELAGVVFELIGKGRELADKLGE
                     QLNVVLLGHKTESMVEQLSKYPVDSIFVYDHQELTYYRPQPYASALEDLISHIKPSIF
                     LIGATHVGRSLAPRIATRLRTGLTADCTVIDVKPNGELVQIRPAFGGNVMAQIVTPNH
                     RPQFATVRPKVMQAAELAPSHPTQVIYRELEPEKLLSNISFLSIEPKPKETSITEADI
                     LICVGRGLRKAEDLAIVEELAEKLGGMVAVTRPLVEAGWAPQTRQVGLSGRTVRPKLF
                     IACGVSGAIQMVAGMSGSEYIIAINKDRNAPIFEVAHLGLVGDMYEILPALVEELGRT
                     LTKSKESR"
     gene            complement(1705..2511)
                     /locus_tag="PLW82_05045"
     CDS             complement(1705..2511)
                     /locus_tag="PLW82_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOJ46672.1"
                     /translation="MRIIVCIKQVPDTTEVAIDEEKGVLRREGVDSRMNPYDLYALET
                     ALRIKEQIGAEVIVLSMGPKQAEDVIKQAYYMGADSGYLLSDRKFAGADTLATAYTLA
                     LGVKKIGDFDLIITGMQTTDGDTAQVGPAVAEFLNLPHISYVKEIQKVAEDQLIVKTD
                     MQDAIETQKVVLPALITVAKEIYQPRLISYKLKLRAKDRNICVWSYSDLVEVKGLLND
                     ETEFFGLTGSPTQVERIFAPTRNSTKEIWEDQPDVLAEKAISKLKELKIL"
     gene            2777..2980
                     /locus_tag="PLW82_05050"
     CDS             2777..2980
                     /locus_tag="PLW82_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46673.1"
                     /translation="MQKEGVGVEKIFDSLTELRKQLEEYLEDKLIDDNDPTLNQILAT
                     IDSLCVELFQKEMERSKSDSDFP"
     gene            3127..>3361
                     /locus_tag="PLW82_05055"
     CDS             3127..>3361
                     /locus_tag="PLW82_05055"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015376076.1"
                     /note="catalyzes the formation of spermidine from
                     putrescine and S-adenosylmethioninamine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HOJ46674.1"
                     /translation="MVGMWFTEDQTQHLLISVRITDILHTEKTPYQQLVVVDTVEFGK
                     MLVLDGAIQCTEKDEFFYHEMIAHVPMHVHPNPK"
BASE COUNT          762 a          818 c          851 g          930 t
ORIGIN      
        1 gcctaaaacg tggtctctgg tgacgccgta ctttacagct cgcatgccgc cggcattggt
       61 catcacgttg ccaccgatgg tggcactctt ttcacccgga tccggtgcgt agaagagacc
      121 gaacctctcg agctcctctt ggaaggagag gagaatcact ccaggttcta ctgtggcagt
      181 gaggttgtct tcatcgatct ccaggaccct gttcatcctg gttgtaacca ggagaattcc
      241 gccatagatg ggtacgcaac ctccgcagag acctgtaccc gagccgcgca ccgtaaccgg
      301 tatcttgttg ctgctggcat acttcataat ggcgctgatc tcttctgctg aggtagggaa
      361 gacgatacag ccgggaaaat cctccatgcc gtagatctca tcgtgggtgt aatctgggtt
      421 tacctcgtct cctgcgaaaa cgaactcttt accgacgatc tgctcaaggt actctaaatc
      481 tgtcttggtg attttataca taaagtcacc tgctctcttt gctctttgtg agggttctgc
      541 ccagctcctc tacaagagca ggcaggatct catacatatc tcccacaagt cccaggtggg
      601 ctacttcaaa aatcggagcg tttctgtctt tgttgatggc aatgatatac tccgagccgg
      661 acatgcccgc aaccatttgg atcgctcctg aaacaccgca ggcaataaac agcttgggtc
      721 ttacggtgcg gccgctgagg cctacctgtc tggtctgggg agcccaaccg gcttctacaa
      781 gaggccgtgt caccgctacc atgccgccga gcttttcagc aagttcttcg acaatggcaa
      841 ggtcttctgc cttcctcaaa cctcggccta cgcagatgag gatatctgct tcggtgatcg
      901 atgtctcttt aggcttgggt tcgatgctga ggaagctgat gttggaaagg agtttttcag
      961 gctcaagttc tctataaatg acttgtgtgg ggtggctggg agcaagttca gctgcctgca
     1021 taactttggg tctaactgtg gcaaactgtg gcctgtggtt tggcgtgacg atctgggcca
     1081 tgacgtttcc gccaaaggcc ggcctaatct gtaccagttc tccgtttggt ttgacatcaa
     1141 tgacggtgca gtctgcagta aggcccgtcc tcagccgtgt agctattctc ggcgcaagag
     1201 acctgccaac gtgagttgcg ccgatcagaa agatgctcgg cttaatgtgg gaaatcaggt
     1261 cctccagagc agaagcatac ggctgaggtc tgtagtaggt cagttcctgg tggtcgtaga
     1321 caaagatgga gtccacaggg tatttggaga gctgttccac catgctttcg gttttatgtc
     1381 ccaagagcac tacattaagc tgctcgccga gtttgtcggc aagctctcgg ccctttccaa
     1441 tcagttcaaa gaccactcct gcaagttctc cgtctctttg ctctgcaaag actaaaacgt
     1501 ttctgtagct cgacaaatca cttttagtga gtgatccttc tgcaactgac agagcttttt
     1561 ccggacagac cttgatgcag actttgcaca gccggcagga gtccagtact tcaagcttgc
     1621 ctgttatctt cagagcgccg aatgggcagc tgtctacaca cactccacag agaatgcatt
     1681 tatcgtgatt tacacctaag tagctcatag gattttaagc tccttcaact tggaaatggc
     1741 tttttcagcg agaacgtctg gctgatcttc ccagatctct ttggtgctgt ttctagtagg
     1801 ggcaaagatc cgctctacct gtgtggggga gcccgtaagg ccgaagaact cggtttcgtc
     1861 gttcagcaac cctttgacct ccaccaggtc gctgtagctc caaacgcaga tgtttctgtc
     1921 cttagcccgg agtttgagtt tataggagat gagtctaggt tggtagattt ctttggcaac
     1981 ggtgatcagt gctggaagaa ctactttttg ggtttcgatg gcatcttgca tgtcggtctt
     2041 tacgatcagc tgatcttctg caaccttttg aatttctttt acgtaggaga tgtggggaag
     2101 gttaagaaac tcggcgaccg ccggtccaac ctgggcggtg tcaccgtcgg ttgtttgcat
     2161 tcctgtgatt atcaggtcaa agtcaccgat cttttttact cctaaagcaa gggtgtatgc
     2221 agttgctaag gtgtccgctc ctgcaaactt cctgtcggag agcaggtagc ctgagtctgc
     2281 gcccatgtag taagcttgtt tgatgacgtc ttcagcttgt ttcgggccca tgctcaggac
     2341 tattacttct gcgccaatct gttctttgat gcgcagcgca gtctctagtg catagagatc
     2401 gtaggggttc attctgctgt caaccccttc gcgcctcagc acgcctttct cttcgtcgat
     2461 agcgacttct gttgtatctg gtacttgttt gatgcagacg attatacgca cgttctcgcc
     2521 tccttttttg taatgtcgta catctgaata tttttacacg cagaaaaaat cccctctcgt
     2581 gagcctgtcc tgttttactc gaaatctagg aggggttaca ggtaaaaaac aggcaatttt
     2641 ctgcaaagtt gttttataac ggtatagatc ccgatttcgg gggttacttc cgtttttcgc
     2701 ccaaaagcga caatttacac tttgagattt tgtgctgtaa actttggtta attgtagaaa
     2761 atttgacgaa aaaagtgtgc agaaagaagg agtgggtgtg gaaaagatct tcgattctct
     2821 gacggagctc cgcaagcagc tggaagagta tctggaagat aaactcatcg acgataacga
     2881 tccaacgctg aaccagatac tcgcgacaat cgacagcctt tgcgtagaac tctttcagaa
     2941 ggaaatggag aggtcaaaaa gcgattccga ctttccctga tccagctttg gagctgggca
     3001 tgtgtcttgt ttgcggggcc gaactggctt ttacgttaga gatgaggtaa tataagtacc
     3061 tgaggagacc aggctttcct acggcccctg gtttgcccaa acacaaattg tgatagaggt
     3121 gattaaatgg tcggaatgtg gtttacggag gatcagacgc agcacctgct tatttctgtc
     3181 aggattaccg atattctgca cacagaaaag accccctatc agcaattggt ggttgtggac
     3241 accgttgagt ttggcaagat gctggtttta gatggagcta tccagtgcac tgaaaaagac
     3301 gagttcttct accacgagat gatcgcacac gtgccgatgc acgtccaccc aaacccaaag
     3361 a
//
LOCUS       DAOSDA010000182         3358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_18111_length_3358_cov_3.446866, whole genome shotgun sequence.
ACCESSION   DAOSDA010000182 DAOSDA010000000
VERSION     DAOSDA010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18111_length_3358_cov_3.446866"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLW82_05060"
     CDS             <1..453
                     /locus_tag="PLW82_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOJ46675.1"
                     /translation="ARIQSDLITKGESPHADVFSPQRLHLAAAPSLVVQNLDVARKLI
                     SGKLSGGAKEEDLKNGEAKVITAYGQKAGAYRDHEGTLHVLDITCTHLGCELVWNDAE
                     LTWDCPCHGSRFSYTGDVVECPALRPLKRLEGDDGASDEKNIIDPKLT"
     gene            complement(485..1462)
                     /locus_tag="PLW82_05065"
     CDS             complement(485..1462)
                     /locus_tag="PLW82_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46676.1"
                     /translation="MNEFFWGGNTGRGSVNFAKEFFPEAKKRILIKGGPGTGKSSFIK
                     RLLKRIENPVVFYCPSAPDSFDTIADFDLGFFVTDATAPHILEAEKPGIRDEILNFGN
                     YWDSSRLRSNQRLVEELSQKNSMHFSLAYACLQKALKFREQLERSLAERLHPSLETQY
                     QKIKDEILNRKLHGKNELGKSRNRFVSAYTPFGFKKLKVPAEKTYTFEGAPTLAKAYL
                     SSLAGIFLTQGFEVLFLIDPLDGMNYEGVYLPELKLHIKVVWPAEHHYTSFERELQGT
                     IKEEERQGIVQLNKAFLVHEDLEKLYIAAMDFDRMEKELDEFTDRLFAD"
     gene            complement(1623..2450)
                     /locus_tag="PLW82_05070"
     CDS             complement(1623..2450)
                     /locus_tag="PLW82_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOJ46677.1"
                     /translation="MLQSEKHRAQQVLFYLVLLVPFMMFTSYALDTLPVINEPSLKML
                     AQAIVLSIIIHGWLRFVLETGNFKGYLEVDGIKKLGIGALFGALVALIVVVLDNLVGT
                     KSRVFNPALLSSAFFRVLVSSIIFFISESYIEEMVTRGYVLEQFEEMHHPHRAVIYSS
                     LVFVLVQFLWDRPHVNLLGWANFYVLAFFLGLLYLCTKAKWLCIGFHTAWNLVFTTLF
                     PVTEVHSTHFASYVHMESWLTFVLLLLLAGVSLYRFEKHHAKPEKKRGLEIVGSRAK"
     gene            complement(2591..>3358)
                     /locus_tag="PLW82_05075"
     CDS             complement(2591..>3358)
                     /locus_tag="PLW82_05075"
                     /inference="COORDINATES: protein motif:HMM:NF025986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ46678.1"
                     /translation="NQKSLAEALDYVVMSAVNHVGVELNTASWSLLRYVSGINKTLAQ
                     RIVAYRQENGPFRSRKQLLEIKGLGAKTFEQAAGFLRVAGGENILDNTAVHPESYELA
                     EKIIGSLGFDLTDVEKPEFKEALKDVDAKELASKFEAGLPTVIDIVDSLQKPGRDPRE
                     DLPGPVFRSDVMKFEDLKVGQVLTGTVRNVVDFGAFVDIGVKRDGLIHVSKLPKGHKL
                     HPTQQISVGEIVEVVVTEIDTKRQRISLKLSKKVSRD"
BASE COUNT          856 a          854 c          774 g          874 t
ORIGIN      
        1 gcaagaatcc agtccgacct gattaccaaa ggtgaaagtc cccatgcaga tgtgttcagt
       61 cctcaacggc tgcatctagc tgctgctcct agcttggtgg tacaaaatct agatgttgcc
      121 cgcaagctga tctcgggtaa gctctccggc ggggctaagg aagaagacct gaaaaacggc
      181 gaggcgaagg tcatcactgc ctacggccag aaggcaggag cttatcgcga tcacgaaggc
      241 acgctccacg tactggacat tacctgcacc catctaggct gcgaactggt ctggaacgat
      301 gcggagctga cctgggattg tccctgtcac ggttctcgtt tcagctacac cggcgatgtg
      361 gtcgaatgcc ctgccctccg tccgcttaaa cgtctggaag gagacgacgg agcgagcgac
      421 gagaagaaca tcatcgatcc caaacttacg taaaaaaatg gatctcgcaa gagatccatt
      481 ttaattagtc agcaaagagc ctatctgtga actcgtctaa ttccttttcc atgcgatcga
      541 agtccatcgc tgcaatgtac agcttttcga ggtcttcgtg aaccaaaaac gccttgttca
      601 gctgaacgat gccctgcctt tcttcctctt tgatggtccc ctgtagttcc ctttcaaagc
      661 tggtgtagtg gtgttcagct ggccacacta ccttgatgtg cagcttcagc tcaggtaggt
      721 ataccccttc gtagttcatt ccgtctaaag gatcgattag gaacaagact tcaaacccct
      781 gggtcaaaaa gatccctgca agagaactga ggtaagcctt cgccagtgtc ggagcacctt
      841 cgaaggtgta ggttttttcc gcaggaactt tcagcttctt gaagccaaag ggagtatacg
      901 cagaaacgaa gcggttgcgg ctttttccca gttcgttttt gccgtggagc ttgcggttta
      961 atatctcgtc tttgatcttt tggtactggg tttctaagct cggatgcaac ctttctgcca
     1021 agctccgttc tagctgctcg cgaaacttca acgctttctg caggcaggcg taagccaagc
     1081 tgaaatgcat cgagtttttt tgcgacagct cctcaacaag cctctgattg gaccttaaac
     1141 ggctcgagtc ccagtagttg ccaaagttca ggatttcgtc tctgatgcca ggtttttcag
     1201 cttctaagat gtggggagcc gtggcatcag ttacaaaaaa accaaggtcg aagtcggcga
     1261 tagtatcaaa tgagtccggt gcagaagggc agtaaaaaac gacgggattt tcaatcctct
     1321 tgagtagtct tttgatgaat gaagatttcc ccgttcccgg acctccctta atcagtatcc
     1381 gtttttttgc ttcgggaaag aactccttag caaagttaac cgatcccctg ccggtgtttc
     1441 ctccccagaa aaattcgttc atgaactcac ctcttaaaac gcactgaact cagtgcggaa
     1501 acttgggtct ttcgcctttc tattcttatg cagtttaccc cattttagtt agcgacactg
     1561 catgtctcga aaaagtgcca agaaaaaaac tgccgaataa aatcggcagt ttctcagctg
     1621 tttcacttag cccggcttcc tactatctcc aggcctcttt tcttttccgg tttggcatgg
     1681 tgcttttcga atctatacag cgacacgcca gccaaaagaa gcagcaggac aaaggtcagc
     1741 cacgactcca tgtgcacgta gctggcaaag tgggtcgagt gcacttcagt taccgggaaa
     1801 agcgtggtaa agaccaggtt ccacgcggtg tggaaaccaa tgcaaagcca cttggcctta
     1861 gtgcagaggt acagtagacc caggaaaaat gctaagacgt aaaagtttgc ccagcctaga
     1921 aggtttacat gaggcctatc ccagaggaat tgcaccagga caaacaccaa agacgagtag
     1981 attaccgctc tgtggggatg atgcatctcc tcaaactgct ccaaaacgta tcccctggtg
     2041 accatctctt cgatgtacga ttcagagata aagaaaataa tactggatac aagcactcgg
     2101 aagaaagcgc ttgatagtag tgctgggttg aacactctag atttcgtgcc cacaagattg
     2161 tccagtacaa ccactataag tgcaacgaga gcaccgaaaa gggcacctat gcccagtttt
     2221 tttataccgt caacctctag atatccttta aagtttccag tttccagtac gaaccttagc
     2281 cacccgtgga tgattatgga cagaacaatg gcctgagcta gcatctttaa gctaggttcg
     2341 ttgataacag gcaaggtatc tagtgcgtac gaagtaaaca tcatgaacgg aacaaggagc
     2401 actaaataaa acaaaacctg ttgcgcgcga tgcttttcac tttgcagcat ttatacagcc
     2461 tccatattac ttccagtgat tcttcaacca ctactagctc agagtccatt gtatacaact
     2521 tcaggaaaca gtgcaactaa aatgctcgct gattttgttc aaaatttgac atttttgacg
     2581 caaatcgctg ttaatctcga ctaaccttct tggacagttt caagcttatc ctctggcgtt
     2641 tggtgtcgat ttcggtaacc acaacctcta cgatctctcc taccgagatc tgctgcgtcg
     2701 gatggagctt gtggccctta ggcagtttgg aaacgtgaat gagaccgtct ctcttcactc
     2761 cgatatctac aaaagcaccg aaatccacaa cgtttctaac ggtccccgtg agaacctgtc
     2821 cgactttgag gtcttcaaac ttcatgacgt cgctcctaaa caccggcccg gggaggtctt
     2881 ctcgtggatc tctacctggt ttctgcagag agtccacaat atctatgacc gtagggagac
     2941 ctgcctcaaa cttagaagca agttccttag catctacgtc tttcaaagct tctttaaact
     3001 ccggcttctc cacgtcggtg aggtcaaatc ccaaagagcc aatgatcttt tccgcaagct
     3061 cgtaggactc ggggtgaact gcggtgttgt ccaagatgtt ttctccacct gcaacacgca
     3121 aaaagcctgc agcctgttca aaggtttttg ctccgaggcc cttgatttca agaagctgct
     3181 ttctggagcg gaaaggcccg ttttcctgtc tgtaggcgac gatccgctgt gccaaggttt
     3241 tgttgatgcc gcttacgtag cggaggaggc tccaagatgc ggtgttcagt tccacgccaa
     3301 cgtggtttac tgcagacatg accacataat ccagtgcttc cgcaagactc ttttggtt
//
LOCUS       DAOSDA010000183         3347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_18192_length_3347_cov_3.161300, whole genome shotgun sequence.
ACCESSION   DAOSDA010000183 DAOSDA010000000
VERSION     DAOSDA010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18192_length_3347_cov_3.161300"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLW82_05080"
     CDS             <1..468
                     /locus_tag="PLW82_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46679.1"
                     /translation="PSASIAYAQLASLLLDTRDYELLEQNSPKAIEMALKGAEVADSG
                     AGKAANYLVISKAYRSVDNYDAAIEAAKKAVEADPTNFKTYLELTINYYEAYTSIEDE
                     DDEAMAKSEAYIYGALENINKAKSLTPYDRDVESYYSRVTRAVERWEQQKNQR"
     gene            534..773
                     /locus_tag="PLW82_05085"
     CDS             534..773
                     /locus_tag="PLW82_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46680.1"
                     /translation="MLQGLEDEAVQIILDFVENRKGVDASMAELSEEEKGELLDLLQW
                     ELGVLLDTESYNELLYGEMRLDNILNAFYDGTPIQ"
     gene            789..1091
                     /locus_tag="PLW82_05090"
     CDS             789..1091
                     /locus_tag="PLW82_05090"
                     /inference="COORDINATES: protein motif:HMM:NF028070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOJ46681.1"
                     /translation="MKETVDERVSLAEKIEIRYKGLLARAGANRIYLHCGFGPEWKNI
                     RDIAMNRDDDNVWKTTLELKDGSEVNFCFRDDAGNWDNNNGKNWGFVVDNSQLISR"
     gene            2383..>3347
                     /locus_tag="PLW82_05095"
     CDS             2383..>3347
                     /locus_tag="PLW82_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOJ46682.1"
                     /translation="MNIKEAALLLHKTHRGKFSVVSKVPVTSKEELSLAYSPGVAAPC
                     LQIAADPESVYEYTAKGNLVAVVTNGTAVLGLGDIGAEAAMPVMEGKAILFKEFGGVD
                     AVPICLNSKDPDEIVRAVELMAPTFGGINLEDIKAPECFYIEDKLKASLDIPVFHDDQ
                     HGTAVVVLAGLRNAAKLVGKELADLNVVINGAGAAGTAIAKLLLSVGVKNIAVLDRTG
                     AIHVDRSNLGDAKAELAKLLPKRTGNLENELVGADCFIGVSSKDVLTQEMIKSMSRDP
                     IIFALANPDPEIDPALAVEAGAAVVATGRSDYPNQINNVLGFPGI"
BASE COUNT          973 a          693 c          865 g          816 t
ORIGIN      
        1 ccatctgcat ccatcgctta cgctcagctg gcatcattgc ttctagacac cagagactat
       61 gagcttctcg agcaaaacag cccgaaagcc atcgagatgg cattgaaggg cgctgaagtc
      121 gcagactcag gagccggaaa agctgctaac tacttggtta tctccaaggc atatcgttcg
      181 gtggacaatt acgacgccgc tatcgaggca gcgaagaagg cagtagaagc agatccaaca
      241 aacttcaaga cttacttgga gctgaccatc aactactacg aagcatatac cagcatcgaa
      301 gatgaagacg acgaagctat ggcaaagtca gaagcataca tctacggtgc actggaaaac
      361 atcaataaag ctaagtctct gacgccatat gaccgcgatg tagaatccta ctacagccgc
      421 gtcaccagag cggtagagag atgggaacag caaaagaatc agcggtaatc taggtataga
      481 cgcctcttcg gctggttagg ctaattttag cctaacaccg aggaggtttt tctatgcttc
      541 agggtttgga ggatgaagct gtacagatca tcttggattt tgttgaaaac agaaaaggcg
      601 tagatgcaag tatggcagag ctgtccgaag aagaaaaagg agagctcttg gaccttctcc
      661 agtgggagct cggagtactc cttgacacag aatcgtacaa cgaacttctc tacggcgaaa
      721 tgcgtttaga taatatcctc aatgcttttt acgatggcac gcctatacaa tagtatggag
      781 gggattagat gaaggagaca gtagatgaga gagtatctct tgctgaaaaa atcgagatcc
      841 gctacaaagg ccttctggct agagccgggg ccaaccgtat ctatcttcac tgcggcttcg
      901 gacccgagtg gaaaaatatt agggatatcg cgatgaacag ggatgacgat aacgtttgga
      961 aaacgacatt agagcttaag gacggctccg aagtcaattt ctgtttccga gacgatgccg
     1021 gaaattggga caacaacaac ggtaagaact ggggattcgt ggtggacaac tcacagctga
     1081 tatcgagata gttaaggcta agcaaaacag cttagcccgc tttttttgca gtgttgcggt
     1141 gaatcgcata aatgcaaaga atgaacaaag acgagccgtt ctaaccgcaa aacttaaaag
     1201 atgctttttc actccgccac ttcccaactg aacataggtg tttcgttaga acattaagtc
     1261 ccgacctggg actttttgtc tgtggtaatc ctggggtgtg ctcgtttttt cctcagacac
     1321 cgttgagatt cactgctaaa acttggtttt gactgggagt ttctgaagat ggaatcgtga
     1381 gtagcaaagg tctttggttt ctgaaggaat atccaggatc accgatatat gtagcaagtg
     1441 ccggaacatg aaagggaccc acaaaatgtg tttgtgttaa tggctcatga cgcgcagctt
     1501 gcaaggacga ctatgagtgc gatttcttac ggatattggt tctatttagt aatacagagt
     1561 ttgctgttat tgcggaaaaa agagttcaga cgcggcattg attgtattgc tcgctttacc
     1621 tgcagcattt aggcttcgta agtatctgtt aaacgttgga gaggcatcgg atgttttgtt
     1681 gatttttttt gtacatggtg ttttacgtaa tcattccagg aatggtgtct gcgattcaag
     1741 cagtttgcga tacatatgat cgcagcaact cgtcggaacg aaaaacgatc tgatattact
     1801 tgcaataact cgtacctcag tgcaacagat taagcgtaat agaaggcagt tggcaaagac
     1861 ttggcagttt tatcgttaat gggttggagt aattttgggt gaggctctaa cgttaggatc
     1921 atgccaagaa ggttgctcag cactacaacg ctgtagagga tgcagtctca cgacaataca
     1981 gtactgcgaa tgacctgaat gcttggtgag aaagtgcgtt ggaagacgac gaaaacttca
     2041 agaagtaggt tttagtctgt aagaaaaggg aacctgaaaa aagggttcct ttttccttat
     2101 tcagcagttc accatgtcag caggcatcgg ggctgtggga aggaggactg tcaagataga
     2161 ggtggtaaaa gtttgaccgg tagctttgtc taacgcaaaa agcttagcca tttttgtttt
     2221 ttaaaggtaa acttatgagt aggagaataa tgggtaaaat tgatttagag aaaacactga
     2281 gctgccgcaa cgacacatca tgtcgacttt ctgcgacagg aagaattagc aggaactagt
     2341 agtatgaggt agaagtattc atagaatgtg gaggaagaat agatgaatat caaagaagca
     2401 gcactgcttt tgcataaaac acaccggggc aagttctcgg tagtgtcaaa ggttccagtg
     2461 acaagcaaag aagaactgag tcttgcatac tcaccggggg ttgcagcacc ctgtttgcaa
     2521 atagcagctg atccagagtc ggtgtacgag tatacggcta aaggcaattt ggtagcggtt
     2581 gtgaccaacg gaacagctgt cctgggtctt ggcgatatcg gtgccgaagc tgcaatgccc
     2641 gtcatggaag gcaaagccat ccttttcaaa gagttcggcg gtgttgacgc agtaccgatc
     2701 tgtctgaact cgaaagaccc agacgaaata gtcagagcag tagaacttat ggctcccaca
     2761 ttcggaggca tcaacctcga agacatcaag gcacctgagt gcttctacat tgaagacaaa
     2821 ctcaaagcat ccctggacat accggttttt catgacgatc agcatggcac cgcagttgtt
     2881 gtactggcag gattgagaaa cgcagctaag ctagtgggca aagagctagc cgatctgaac
     2941 gtcgtcatca acggtgcggg agctgcaggt acggctattg ccaagcttct tttaagcgtc
     3001 ggagtgaaaa acatcgccgt actggatcgc acaggtgcaa tccacgttga cagaagcaat
     3061 ttgggtgatg cgaaagccga actggcaaaa cttctgccca agaggaccgg aaacttggaa
     3121 aacgagctcg tcggagcaga ctgtttcatc ggagtttcca gcaaggatgt cctcactcag
     3181 gaaatgatca aatcgatgag tcgggacccg atcatctttg ctctagccaa cccagatccg
     3241 gagattgatc cagctctggc cgttgaagcc ggcgcggcgg ttgtcgctac aggacgctcg
     3301 gactatccca accaaatcaa caatgttcta ggttttcctg gaatctt
//
LOCUS       DAOSDA010000184         3313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_18446_length_3313_cov_3.143339, whole genome shotgun sequence.
ACCESSION   DAOSDA010000184 DAOSDA010000000
VERSION     DAOSDA010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18446_length_3313_cov_3.143339"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLW82_05100"
     CDS             <1..638
                     /locus_tag="PLW82_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOJ46683.1"
                     /translation="GVMYQGRERTPTSPGKISIWAWAAMRIMDYVQTLECVDLKNVAV
                     VGHSRLGKTALVAGAFDERFAFAISNNSGCSGAAISRDKQGEDIRVIATIRPYWFCPN
                     YQKYIDNEHSMPFDQHFLIAAIAPRKVYVSSAVEDVWADPYSEYLACVAASPAFELSG
                     EKGFVHPDRFPEVGEKLHEGWVGYHLREGTHYFSRYDWQQFMDFIKKHKNM"
     gene            complement(721..1299)
                     /locus_tag="PLW82_05105"
     CDS             complement(721..1299)
                     /locus_tag="PLW82_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOJ46684.1"
                     /translation="MRTVSAVLCVLLLCSMALAAPKVLFEDDFTGDLDQWIMTNAGWE
                     IVDEALEFPIVGFSDIFTGEDWDDYVVEAEMTPLEYGQWGSVRLFFRSYEPFTGYGLS
                     FHEGGWIVHCFLGTYDQYRVLAEGLDLIVKPGQKVHVRMEMKGNKFGLYLDGTLVAEF
                     EDPEDTYFDGGIGFRADNAALRVDNVKVTALR"
     gene            complement(1370..2656)
                     /locus_tag="PLW82_05110"
     CDS             complement(1370..2656)
                     /locus_tag="PLW82_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46685.1"
                     /translation="MKRLALPLILLLVLSVLAYGTQIEVWLVGLTGEMAGILSDLAYE
                     TFTSQTGIEVNIYILTWADYYNRSLLALASRDTPDIFAVGSEVMDFGVRGGLVDLAKF
                     KPDELKEVEAQLFGALMGPVSFHGTRFGVPYDSGGMVTAYRTDILADMGMAVPVTWDD
                     IYNWQPKANAQGRTFGLYYGSVDYASEWGAYTLVTQAGGQFFSEDGFSSALDRPESVV
                     GFKNYVELFTKYNLPRAGVGLEPFINGEWLMLTDGAWLHSNLIKGAPQLSGKWAIDLI
                     PGTKRPNGEINHGSFVGGTYLAISSLSKNKEEAWEFIKWMTSAEMQRRYANTVLERIT
                     GSIWLPANRQALREIAVDDSFRETFVKQAEQSAPVPPAINVAVLYRYVKFAVDKSVLS
                     GVDPEVAILDAAKEMNEDMGKRRKEYTRYLQELGVF"
     gene            complement(2693..>3313)
                     /locus_tag="PLW82_05115"
     CDS             complement(2693..>3313)
                     /locus_tag="PLW82_05115"
                     /inference="COORDINATES: protein motif:HMM:NF027473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4855 domain-containing protein"
                     /protein_id="HOJ46686.1"
                     /translation="KSLNFAGITTGDGNRFLAVKWFVEEVLTRWEEAELPNLELIGFY
                     WYQEDIESRNSPEIFDPVIKNTADLLHEKGLYFYWIPYYQARGFRNWQDLGFDVAMMQ
                     PNFSFSRLDLSEAEYRLTQAAELASRYGLGLQMELHPDLQNRSMREKYLAYLEYGTKL
                     GYQNNSVICYYQGVDDFAKAAFSVDPEIRYLYDATYKFVKGEQIGD"
BASE COUNT          820 a          894 c          774 g          825 t
ORIGIN      
        1 ccggagtgat gtatcaggga agagagagaa cccctacttc acctggtaaa atctccattt
       61 gggcatgggc agcaatgcgg atcatggact acgtccaaac tctagagtgc gtagatttga
      121 aaaacgttgc ggtggttggc cactcaaggc tagggaaaac cgcactggtt gccggtgcat
      181 ttgatgagcg ctttgccttt gctatctcca acaactcagg ctgcagcgga gctgccattt
      241 ccagagataa gcagggtgaa gacatcagag taattgcaac catccgcccc tactggttct
      301 gtcccaacta tcagaagtat atagacaacg agcattccat gccctttgat cagcatttcc
      361 ttatagctgc cattgcccca cgaaaagttt acgtatctag tgccgtcgaa gacgtgtggg
      421 cagacccgta ctctgagtat ctagcctgcg ttgccgcaag tcctgctttc gagctttcag
      481 gagaaaaggg cttcgtccat cctgaccgtt tcccagaggt aggagaaaag ctgcatgaag
      541 gttgggtagg ctaccactta agagagggca cccattactt cagcaggtat gactggcagc
      601 agttcatgga tttcatcaag aagcacaaaa atatgtaata acgaggcaaa tacaaaggga
      661 accgataagc acaaaactta tcggttccct tttcctttta tcgccctaga aggcgcagaa
      721 ctatcttaat gcagtgactt ttacgttgtc gactcttagt gcagcgttat cggctcggaa
      781 gcctataccg ccatcaaagt aagtgtcttc cgggtcttcg aactctgcaa ccagtgtacc
      841 gtcaaggtac aaaccaaatt tgttgccttt catttccatg cgaacgtgaa ccttctgacc
      901 tggctttacg atcaagtcca atccttcggc caaaactctg tactggtcgt atgttcccag
      961 gaagcagtga acgatccacc caccttcatg gaaggaaaga ccatagcctg tgaaaggttc
     1021 gtaggaacgg aagaacaacc tgacagaacc ccattgaccg tactccagag gagtcatctc
     1081 cgcttccaca acgtagtcgt cccagtcttc tccagtaaaa atgtcgctga atccgacgat
     1141 tgggaactct agagcttcgt cgacgatctc ccaacctgcg ttggtcatga tccactgatc
     1201 gaggtcgccg gtgaaatcgt cttcaaacaa taccttcggt gcggccagtg ccattgaaca
     1261 caggagaagt acacacaaaa cagctgacac agttctcatt gcatttcccc tccaaaacat
     1321 gttattagac catacagtta ggtttgtccc actttacaga agttccttct tagaatacgc
     1381 ctagttcctg caggtagcgc gtatactctt tgcggcgctt acccatgtcc tcgttcatct
     1441 ctttagcagc gtccaagatc gctacctcag gatccacacc acttaggacg ctcttgtcca
     1501 ccgcaaactt cacgtaacgg tagagaactg cgacgttgat cgcaggtggc acaggcgcgg
     1561 actgctctgc ctgcttgaca aacgtctcgc gaaagctgtc gtcaactgcg atttcccgca
     1621 gtgcctgacg gtttgcagga agccaaatac ttccagtgat cctctctaaa accgtgttag
     1681 cataccgcct ctgcatttca gcagatgtca tccacttaat aaactcccag gcctcttctt
     1741 tgttttttga taggctggag atggcaaggt aagttccacc gacgaacgat ccgtggttaa
     1801 tctcaccatt tggccgcttg gtccctggaa tcaggtcgat agcccacttg ccagacagct
     1861 gcggagcacc cttgatgagg ttggagtgca gccaagcacc gtctgtgagc atgagccatt
     1921 caccgttgat aaacggttcc agacctactc cagctcttgg caggttgtat ttggtgaaga
     1981 gctccacgta gtttttgaag cctactacag actcaggccg atccagagca ctgctgaacc
     2041 cgtcttccga gaagaactgc cctcctgctt gagtcaccaa agtgtacgct ccccattcag
     2101 aggcgtagtc tacagaaccg tagtacaggc caaacgttct gccctgagcg ttagcttttg
     2161 gctgccagtt gtagatatcg tcccaggtga cgggaactgc catacccatg tccgcaagaa
     2221 tgtcggtcct gtaggccgtg accatccctc cactgtcgta gggaacacca aagcgagtgc
     2281 cgtggaagga taccggtccc atgagagcac caaagagctg cgcttccact tctttcagct
     2341 catctggctt gaacttggcc agatctacca gtccaccgcg aacgccgaag tccataactt
     2401 cactgcccac tgcaaagata tcgggcgtat ctctgcttgc tagggctagc agggatctgt
     2461 tatagtagtc ggcccaagtc aaaatgtaga tgttcacttc aattccggtt tggctggtga
     2521 acgtttcgta ggccaggtca gagagaatcc ccgccatctc gcctgtaagg cctacaagcc
     2581 acacctcgat ttgcgtacca taggcaagta cgctcagtac aagcagcaag atcaaaggta
     2641 atgctaaacg tttcatagca accctcctaa taagctgtgg tatactcgca cactaatcgc
     2701 cgatctgctc gcctttgacg aacttgtagg ttgcgtcata caagtacctg atctcaggat
     2761 ctacgctgaa cgcagcttta gcgaagtcgt ctactccctg gtagtagcag atcaccgagt
     2821 tgttctgata cccgagtttt gttccgtact ccaagtacgc caagtacttt tctctcatgc
     2881 tgcggttttg caggtctggg tgcagctcca tctggagacc gagcccgtac cgggatgcaa
     2941 gttctgcagc ttgagtcaat ctgtactctg cttcggaaag atccagcctg gagaaggaga
     3001 agttgggctg catcatggca acgtcaaagc cgagatcctg ccaatttcta aaacctctag
     3061 cttggtagta agggatccag tagaagtaaa gacctttttc atggagcaga tctgcggtgt
     3121 ttttaatcac cgggtcgaag atctccgggg agttcctgga ctcaatgtct tcctgatacc
     3181 agtaaaagcc tataagttcc agatttggca gttctgcctc ttcccacctt gtcagtactt
     3241 cttctacaaa ccactttacc gccaggaatc tgttgccgtc ccctgtcgta atcccggcaa
     3301 aattcaggga ctt
//
LOCUS       DAOSDA010000185         3291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_18618_length_3291_cov_2.898640, whole genome shotgun sequence.
ACCESSION   DAOSDA010000185 DAOSDA010000000
VERSION     DAOSDA010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18618_length_3291_cov_2.898640"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PLW82_05120"
     CDS             <1..362
                     /locus_tag="PLW82_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ46687.1"
                     /translation="QVLFFQMAPVDQRHAFEVAREVATDPRIQTADERILLVKAGLLH
                     DIGKVKGDFTITQRVLVKLMKKFLPGTYRKLASQYQKPGTLTHAVFVQAAHPKRGAYM
                     LQAFGASELVEKPPRNT"
     gene            426..1175
                     /locus_tag="PLW82_05125"
     CDS             426..1175
                     /locus_tag="PLW82_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOJ46688.1"
                     /translation="MSIDVSVKAFEGPLDLLLSLIKEKQIDIYDIPIAEITAAYLEEL
                     KNMESSNYEVAAEFVVMAATLMEIKARMLLPKAKIEEEEEEEDPRTELVNKLLIYKQF
                     KEAANFLKESFEDQSRVFARDTINRLLLEELKPDHDLRNLGVSISDLLGALRVVLEAK
                     KEEEEILPVLPREKITIDEQVNWLTLYMKEKKDLLFSDLFGVLKSKVLIVVTFMALLE
                     LVHKGTVSVRQSEDLEIYVSYIENGGESEEV"
     gene            1181..1738
                     /gene="scpB"
                     /locus_tag="PLW82_05130"
     CDS             1181..1738
                     /gene="scpB"
                     /locus_tag="PLW82_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOJ46689.1"
                     /translation="MSPNEAKKILEAILFMAPEPLSAAKLAELTGLAENEVRSFLLEI
                     EHDLVDQDRGVRVETIAGGYRFIACKEAEPYLLELSQGQKRSPLSPAALEVLAIISYL
                     QPITRAEIDEIRGVSSESPIATLLDRGLIAEVGRKDAPGRPILFATTKAFLVYFGLKD
                     LSELPSLPELKMPNGENTKPPTLVE"
     gene            1761..2339
                     /locus_tag="PLW82_05135"
     CDS             1761..2339
                     /locus_tag="PLW82_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46690.1"
                     /translation="MWWIFGIGMLFLAVKVVLSLPLHMKFTINTDPFGRNWNMETRLP
                     FCRLRQSGEETSEIAEHVSGLHRLFFIYLRNLYHNAVRAKPKKSRIGSEVWESAFFKS
                     LRFKKIALSMGLKEVPPGEAVFIFGVVTAAIYPFMGAISVNNKGLMPEISFKTDLSGF
                     AFTCIITFTLGQVIHEAGKRYWALWKESRYAG"
     gene            2329..2724
                     /gene="ytfJ"
                     /locus_tag="PLW82_05140"
     CDS             2329..2724
                     /gene="ytfJ"
                     /locus_tag="PLW82_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HOJ46691.1"
                     /translation="MPDKHPVEGLMDTAMQSLKEMIDVNTILGDPVETIDGSLIIPVS
                     RVFMGFAAGGSEFGEPNPEAKSPFGGGSGAGISVRPVAFLVVSQGKVKLLPVGTEAFI
                     DRVFDLVPNLVDRMTKNSVTSTVDNNTMF"
     gene            3094..>3291
                     /locus_tag="PLW82_05145"
     CDS             3094..>3291
                     /locus_tag="PLW82_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOJ46692.1"
                     /translation="MERLQKILAEAGYGSRRKVEKLIIEGVVTVNGQVAELGQKASRF
                     DDIRVKGEKIILQRKVYYLLNK"
BASE COUNT          920 a          762 c          850 g          759 t
ORIGIN      
        1 aacaggttct gttttttcag atggcccctg tggatcagag gcatgccttc gaagtggcaa
       61 gagaggtcgc aactgatccg cgcattcaaa ccgctgacga aagaattttg cttgtaaagg
      121 cgggtctcct ccacgacatc ggcaaggtta aaggcgactt caccatcacc cagagggtgt
      181 tggtgaagtt gatgaaaaaa tttctgcccg gaacctacag aaagctagct tcgcaatatc
      241 aaaaaccggg tactttaacc catgcggttt tcgttcaagc agctcatccc aagcgtgggg
      301 catacatgct tcaggctttc ggtgcttctg agcttgtgga aaaaccacca cgaaacacct
      361 aaaagcaggt ttgccaggct gctcagggaa gcggacaact tacactagct taggagggct
      421 ttactatgag tattgatgtg tcggtcaaag cctttgaagg ccctctggat cttctcttga
      481 gcctcatcaa agaaaagcag atcgacatct acgacatacc tatcgcagag attacagcag
      541 catacctaga agaactgaag aacatggaga gctccaacta cgaggtagca gctgagtttg
      601 tggtaatggc cgcaactctc atggaaatca aagcaaggat gctccttccc aaagccaaga
      661 tcgaagaaga agaggaagaa gaggatcccc gtacagaact ggttaacaag ctgctcatct
      721 acaaacagtt caaagaagcg gccaattttc ttaaagaaag ctttgaagat cagtctcggg
      781 tttttgccag agacaccatc aaccgtctcc ttctagaaga gctgaagccc gaccacgatc
      841 tcagaaactt gggcgtttcc ataagcgatc ttctcggcgc tctcagagtg gtactcgagg
      901 ccaaaaaaga ggaagaagag atccttccgg ttcttcctag ggagaagatc actatcgacg
      961 agcaggtcaa ctggctcact ctctacatga aggagaaaaa agatctgctc ttcagcgatc
     1021 tttttggcgt tttaaaaagc aaagtgctca ttgtggtaac gtttatggcg ctattagagc
     1081 tggttcacaa aggcactgtc agcgtcagac aatcagagga tttggagatc tacgtcagct
     1141 acattgaaaa cggaggcgaa agcgaggagg tctgagagac ttgtccccaa acgaagccaa
     1201 gaaaattctc gaagccatac ttttcatggc acctgaacct ttgtccgcgg caaaacttgc
     1261 agagctaacc ggtctagcag aaaacgaggt caggagcttc cttttggaga tcgagcacga
     1321 ccttgtggac caggataggg gcgtaagagt ggagaccatc gcaggcggtt atcggtttat
     1381 agcctgcaag gaggcagaac cctaccttct tgagctcagc caaggccaaa agcgcagccc
     1441 tctttcgcct gcggctctcg aagttctggc catcatcagc tacctgcaac ctatcaccag
     1501 agcagagatc gatgagatcc gcggcgtttc ttctgaaagc cccatcgcta cgctgttaga
     1561 caggggtctt atcgccgaag tgggcaggaa agatgccccc ggaaggccga ttctctttgc
     1621 cactaccaaa gccttcctgg tgtatttcgg ccttaaagac ctgagtgaac tgccaagcct
     1681 ccccgaactc aagatgccta acggcgagaa tacaaaacct cccaccttgg tagaataaag
     1741 cagggtagga ggtgttttct ttgtggtgga ttttcgggat aggaatgctt tttcttgctg
     1801 taaaggtggt tttaagcctt cctctacaca tgaaatttac cataaacact gacccctttg
     1861 gcaggaactg gaacatggaa accaggctcc ctttttgcag actcaggcag agcggagaag
     1921 aaacctccga gatcgccgag cacgtttccg ggctccaccg cctgtttttt atctacttac
     1981 gcaacctcta ccataacgcc gtgcgcgcta agcccaaaaa aagcaggatc ggaagcgagg
     2041 tttgggaaag tgccttcttt aagagcctgc gcttcaaaaa gatcgctctt tccatgggac
     2101 taaaagaagt acctccaggt gaagcggtct ttatctttgg cgtcgtcacc gcggcgatct
     2161 acccgttcat gggtgccatt agcgttaata ataagggact tatgcctgaa atttccttta
     2221 aaaccgacct ctcgggattt gcctttacct gcataatcac ctttaccctc ggtcaagtta
     2281 tacatgaagc cggaaaaaga tactgggctt tgtggaagga gagtagatat gccggataaa
     2341 catccagtgg aaggtttaat ggacactgcc atgcagagcc tgaaagaaat gatagatgta
     2401 aataccattc tcggcgatcc agtggagact atagacggga gtctgataat cccggtatct
     2461 agagtgttta tggggtttgc tgccggcgga agcgagtttg gcgagcccaa tcccgaggct
     2521 aaatctccct ttggcggagg gagcggtgcg gggatttccg tgagacctgt ggcctttctc
     2581 gttgtgagtc agggcaaagt taagctgctg ccggtaggca ccgaagcctt tatcgacaga
     2641 gtgttcgacc tggttcccaa tctggtggac cgcatgacga aaaacagcgt taccagtacg
     2701 gtggataaca acaccatgtt ctaggcattt agaccttaag gggctcagga actctttaag
     2761 gtcttttttt tgtgggagga gttgcagaaa atggaaattc tgcaggtaaa tgtggtatta
     2821 tctagctaac aacacaaaag cagcataact gcggatcgca gagctttaaa ccggttctaa
     2881 agatccagca cgaccgagct gttaaaaaga gtttagtacc tcggtttgca ggggcgtttt
     2941 gcccgacgca tcttttaggc acaagctgca ggaaaaattg gcagggagaa cctgtgccgg
     3001 aaaagagtgc tgcaccgaag cagaggtgtt ggacgacagg tgaaaaagcc cgaaagacca
     3061 ggctttgtca aatctgcgta ggaggatttt agcttggaaa gactacaaaa gatcttagcc
     3121 gaagctggat acggttcaag acgtaaggtg gaaaagctca tcatcgaagg tgttgtgacc
     3181 gtaaacgggc aagtagcaga gctgggccag aaagctagta gattcgatga catcagagta
     3241 aaaggggaaa agataatcct acagaggaag gtctactatc tgctcaacaa g
//
LOCUS       DAOSDA010000186         3285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_18656_length_3285_cov_3.460062, whole genome shotgun sequence.
ACCESSION   DAOSDA010000186 DAOSDA010000000
VERSION     DAOSDA010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18656_length_3285_cov_3.460062"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(436..3237)
                     /locus_tag="PLW82_05150"
     CDS             complement(436..3237)
                     /locus_tag="PLW82_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46693.1"
                     /translation="MKKLLILILLCVFAASAFSYEELLLEATIKYSDGKIKWQDIIDA
                     VDDGGLLIPIFQVAPALDLDVSFDRVNDIFFVTWQENTLPFSSTGYDNLPLLLDKMPV
                     ELAGDYYVHMEFFQSLMGITYLYDPLKMSLVIVLETPSPVVEQVESEELELEKTPVVV
                     YEEPLSVLRPSSIEYQLEKTYRRGSSQTKGQFYLHFRSGLAEISIGAALDNNKLKNPY
                     FLLTYENNNMLFKLGHTSFTSSTRLGNVSLFGLQVISPYPVIPRNYPLAQIEIQGKKG
                     DTAVIFVNNTPAKKVELKSNNPETVLVALKPYRYQVITVENAKILSKKISYNGVSNLN
                     RGESQFNLYAGIFGKRQFEHLGELAELSYSRGLTDNIDFNLGFVWQRLGTVLEKPLLR
                     SALQFELFSGVSLASNVYLVGKSVGGDASLITTFRTGYLELGYFNNPRDVLAYYQKPT
                     GEGASLFLEKDLWSNLRLQGSAGVTFKTDTTPLLRYYKGSASLSFNKPLSRFSLSYGE
                     SENYLAQLEEVLRTKTLTAGMSLAFSRLSLSTKFSDQSFKLESLSWKQRQLQGKVTAN
                     LWKRLYLSSEAFVLEQEGALSGAGELQLGLSLPRSVNLNGAFDIQGKLRPDEELENMT
                     ASLALSFYLLGPHWEYKVQKNEGKRGDYTEYSGKVSLGNKDTQFWLSAALRQTAQKSL
                     ESTFKGYFGHTFASGLTLGLKYEKLPSSPLTGEQEHVASITISQGISFSAKGYLGHKY
                     TGENLATTLAGTVFLDLNGNGLQDEEEPGVPNIEMRIGSKTATTNQDGFFLFSDLSPQ
                     ITMFGFNPDKLTTDYVAPLLEMPVRIRTGDNIVLDMPLTMNGVLKGRVFIDTNLDGIY
                     NDQDILLDWVQIKVLESEKQAFTNKNGNYYVEALPLGSFTVVVEPKSLPLGLKTPDPV
                     RIHITPDALDVIVDFPLVY"
BASE COUNT          808 a          730 c          857 g          890 t
ORIGIN      
        1 gatttgaaac ctgaagttat tgctgcataa tgtccaaagt taggggtttg aaccgagcac
       61 ttttctaaga atcgttgggt cttttttact catcttaacg ttgattggca ctattgtgac
      121 ttgggtactc gcaggctttt ggtacgcgct ttatgcttgt ttaactggat cagcaatctc
      181 tttcgttccg ttggttttag ctgacgtaag ggacagactg gaacgagtcg aatacagact
      241 aggtcaattg aagacatcaa gacgttcgga acagcaagtg gacaccaggt caatgttcag
      301 ttcgcaggag aaggaggtat ccccttcgga gcgcttgttc gatccgattg aggagtagct
      361 caaggagcga gtatcgtagg atggagttcg caacagcaaa aaagccagtt ggttgaaaaa
      421 accactggct ttttttcagt aaacaagggg gaaatccacg attacgtcaa gagcgtcggg
      481 tgtgatgtgt atccgtaccg gatcaggtgt cttcagacct aatggaagtg atttcggttc
      541 aacaaccaca gtgaagctac ccaggggcag tgcctccaca tagtagttgc cgtttttgtt
      601 tgtaaaagct tgtttttccg actcaaggac ttttatctgc acccaatcta agagaatatc
      661 ctgatcgttg tagatgccgt ccaaattggt gtcgataaac actctgcctt tgagcacgcc
      721 gttcatggta aggggcatat ccaggactat attgtcgcca gttctgattc tcaccggcat
      781 ttctaacagg ggtgcaacat agtctgtggt gagtttgtct ggattgaaac cgaacatggt
      841 gatctgaggg ctgaggtcgc tgaacaagaa gaaaccgtcc tggttggttg tagcggtttt
      901 ggagcctatc ctcatttcga tgttgggcac tccgggctct tcttcgtcct gtaagccgtt
      961 tccgttaagg tccaaaaaga ccgttccagc gagagtggta gccaggtttt ccccggtgta
     1021 cttgtggccc agatagccct tggcggaaaa tgaaattccc tgcgaaatgg tgatagatgc
     1081 tacgtgctcc tgttctccgg taagtgggga ggacgggagt ttttcgtact tcagtcccag
     1141 cgtcaagcca ctggcaaatg tgtggccgaa ataccctttg aaggtgctct caagggactt
     1201 ttgagccgtc tgtcgcagcg ctgcggacag ccaaaactga gtgtctttgt ttcccagaga
     1261 gactttgcca gagtactcgg tgtagtcgcc tcgctttcct tcgttcttct gaaccttgta
     1321 ctcccagtgt ggacctaaaa ggtagaaact gagggctaaa gaagcagtca tgttctctaa
     1381 ctcctcgtca ggtcttaact tgccctggat gtcaaaagca ccgttaaggt tcacgcttct
     1441 cggaagactt aagcccagct gcaattcacc ggcgccggac aaagcacctt cttgttcgag
     1501 gacaaaagcc tcgctgctta gatacagccg cttccacagg ttggctgtga ccttgccctg
     1561 gagctgtctt tgcttccagg aaaggctttc cagcttgaaa gactgatcgg agaacttggt
     1621 actcaagctg aggcgggaaa acgccaaact catacctgca gttaaggttt ttgtgcgtag
     1681 gacctcttcc aattgggcga ggtagttttc gctttcgccg tagcttagag aaaaacgaga
     1741 aagaggtttg ttgaagctta aagacgcaga gcctttgtag tagcgaagta gcggagtggt
     1801 atcggtctta aacgtcacac ctgcgctccc ctggagtctt aggtttgacc agagatcttt
     1861 ttccaagaat aagctggccc cttcacctgt gggcttttga tagtaggcta gaacgtccct
     1921 cgggttgttg aagtagccaa gctctaaata tccggttcta aaagtggtga taagagaagc
     1981 gtctcctcca acgcttttgc cgactaaata aacgttagaa gcaaggctca ctcctgaaaa
     2041 cagctcaaac tgcagtgctg aacgaaggag gggtttttcc agaacggttc ctaatctttg
     2101 ccagacaaaa cccaggttaa aatcgatgtt atcggtgaga ccgcggctgt aggagagttc
     2161 tgcaagctct ccgagatgtt cgaactgcct cttgccgaag atgcctgcgt agaggttgaa
     2221 ctggctttct ccacggttga gattagaaac gccattgtag gagatcttct tgcttaagat
     2281 cttggcattt tccacagtga tcacttgata ccggtagggc ttaagggcta ccagcacggt
     2341 ttcgggattg ttagatttca gttcgacttt tttcgcggga gtattgttaa caaagatcac
     2401 cgctgtgtcg ccctttttgc cttggatctc gatctgcgca agaggatagt ttctggggat
     2461 cacagggtaa ggtgagataa cctggagtcc aaacagagaa acgttgccca gtcttgtgct
     2521 cgaggtaaac gaggtatggc cgagtttaaa aagcatgttg ttgttctcgt aagtaaggag
     2581 gaagtagggg tttttcagct tgttgttgtc caatgctgca cctatggaga tctccgctaa
     2641 accagagcga aaatgaagat aaaactggcc tttcgtctgg ctggagcctc tgcggtaggt
     2701 cttttccaat tggtactcga tggaggaagg acgaaggacg ctgagaggct cttcgtagac
     2761 caccacaggg gttttttcta gttccagttc ttcactttca acttgctcca ctactgggct
     2821 gggagtttcc agaacaatca ctagggacat tttcagggga tcgtagaggt aggtgatgcc
     2881 catgagactc tggaaaaact ccatgtgtac atagtagtca cctgcaagtt ctacgggcat
     2941 tttgtccaaa agcagtggca ggttgtcgta tcccgtgctg gaaaagggga gggtattttc
     3001 ctgccaggtc acgaagaaaa tgtcgttgac tctgtcaaag gagacgtcaa gatcaagggc
     3061 aggtgctacc tgaaagatgg ggattaacag accgccgtca tctacagcat caataatgtc
     3121 ctgccatttg atctttccgt cggagtactt aatagtagct tccaaaagca gctcttcgta
     3181 actaaacgcc gaagcagcaa acacgcacag cagtattagt atcagtagtt ttttcatgag
     3241 ccacttccta aaactctgaa cacgatcgcc tgctcgtggg ttaca
//
LOCUS       DAOSDA010000187         3275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_18736_length_3275_cov_3.044099, whole genome shotgun sequence.
ACCESSION   DAOSDA010000187 DAOSDA010000000
VERSION     DAOSDA010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18736_length_3275_cov_3.044099"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLW82_05155"
     CDS             <1..426
                     /locus_tag="PLW82_05155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ46694.1"
                     /translation="LAGELGILNQVIFAGERPWEEIGLYYNVGDVFVSASTSETQGLT
                     YIEALAAGLPLVVREDPCLRNVVQDGVNGYTFKDRNEFISRLDSVLKDPEHRKKLSLE
                     AEKSAKKFSVEHFGETVLGLYSALLSERSQAGEETVRVD"
     gene            422..766
                     /locus_tag="PLW82_05160"
     CDS             422..766
                     /locus_tag="PLW82_05160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019376920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ46695.1"
                     /translation="MEKHHLGVRAFQVHKSKWEVLRMEIKKGTNRFYIGDSENAAIAE
                     ITFVPKGASVIEVDHTWVSPELRGQGIAKLLVDRVVSWAREENLKIIPVCPYVKTQME
                     RNQEYHDLLFGE"
     gene            968..2149
                     /locus_tag="PLW82_05165"
     CDS             968..2149
                     /locus_tag="PLW82_05165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOJ46696.1"
                     /translation="MLKFRIGPSCLLIWLMILCVSGLALAQPVVTAPSAILIEANGGQ
                     VLWEKNGDDRRHMASITKIMTMLLILEAMENGQFGLDDMVTVSENAKRQGGSQIWLEV
                     GEVMSVKDLLYSVAVGSANDSAVALAEFHSGSEAAFVEKMNAKAAELGLTNTHFVNSN
                     GLPESSHRAIDGDHYSSARDIAKLTVEAYKYPLFAEMVSTYEYTVRPGQKGEVVLYNY
                     NKMFLRGYPGADGVKTGMIEAAGYCLSGSAVRDGLRLIAAVLGAKSNNDRYRDMVSIL
                     DYGYNNYKSLVLANAKEQIAEIKVSHGRREVLELLAKQDLRVTVKKVGDETPTLDVHV
                     PEVVKAPITEGESLGVIYAKLGGEVVGEVELVAARGIEKAGLVLEISRSFLQLLGINK
                     L"
     gene            2210..2551
                     /locus_tag="PLW82_05170"
     CDS             2210..2551
                     /locus_tag="PLW82_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ46697.1"
                     /translation="MPVSVRKIGTMLVAEVVGDLDMVTGAQLKEAADQMWEHYETLSV
                     FVLDLSKVGFNDSSGLGAILGRYKKVNARSGTMIIVGAQGSVREDLEISGISKLCLFK
                     ESLKEVAPAQE"
     gene            2614..3051
                     /gene="spoIIAB"
                     /locus_tag="PLW82_05175"
     CDS             2614..3051
                     /gene="spoIIAB"
                     /locus_tag="PLW82_05175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004437536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HOJ46698.1"
                     /translation="MTQKNEVHFQFPSLPVNIAFARSTVAAFAVQIEQFTEAEYNEIR
                     KAVSEAVSNCVQHAYPDSVGTVSISVYIEGDLLTIVIEDQGVGIENVELAKTTEFTTK
                     PDENLGWGFNFMEACMDEISVESYPGKGTKLTMKKRPHANDAQ"
     gene            3041..>3275
                     /locus_tag="PLW82_05180"
     CDS             3041..>3275
                     /locus_tag="PLW82_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ46699.1"
                     /translation="MPNDIELLRQAKAGSKEARDRFLENNTALVVHVAKRFSAGEYED
                     LVQAGFVGLIKALDSFDPGFGTAFSTYAVPYIMG"
BASE COUNT          876 a          711 c          943 g          745 t
ORIGIN      
        1 cttgctgggg aactgggcat tttgaaccag gtgatctttg ccggggagcg tccctgggag
       61 gagatcggcc tctactacaa cgttggagac gttttcgtca gcgcatccac cagcgaaacc
      121 cagggactga cttatatcga ggccttggct gctggactgc ctttggtagt gcgggaagac
      181 ccatgcctgc ggaatgtagt acaggacggc gtaaacggct acacgttcaa agacagaaat
      241 gagtttatct cccgcttgga cagcgtcctt aaggatccgg agcacagaaa gaagctgtct
      301 ctagaagcgg agaagtctgc aaagaagttt tcagttgagc atttcggcga aaccgttctc
      361 ggtctgtact cagctctcct ttcagagcgc agccaagctg gggaagaaac tgtgagagtg
      421 gattgaaaag caccatttag gggtaagagc ttttcaagtc cacaaaagca aatgggaggt
      481 gctgcgcatg gagatcaaga agggaaccaa taggttttat ataggcgact ctgagaacgc
      541 tgccatagcc gagattacat ttgtccctaa aggagcttcg gtcattgagg tggaccacac
      601 ctgggtgtcg ccggagctaa gagggcaggg tattgcaaag cttctcgtgg acagagttgt
      661 cagctgggca agggaagaaa acttgaagat cattccggtg tgcccgtatg taaaaaccca
      721 gatggaaaga aaccaggagt atcacgacct gctttttggt gagtgatgcg tcctgagttg
      781 gaaaccatta gggcattctg ctgaaaactt aggcagaatg cccttttttg aaacatcttt
      841 cccgcaaggt gcctcgagca gagctccgca tgtacgcctg tgaagcgtgg atgccgattg
      901 caaacatacg acaagctgaa gatgcataag attaatcgct aagatttgca agggaggctt
      961 gacttgcatg ctcaagttca ggatagggcc gagctgcctt ctcatatggc tgatgattct
     1021 ctgcgtatcg ggattggctc ttgctcagcc ggtggtcacc gcacctagtg ccatactcat
     1081 cgaggccaac ggagggcagg tgctttggga aaaaaacggc gatgatagaa ggcacatggc
     1141 aagcatcacc aagatcatga ccatgctgtt gatcctggaa gcgatggaaa acggtcagtt
     1201 cggtctggac gacatggtga ccgtaagtga aaacgccaag cgccaaggag gatcgcagat
     1261 ttggcttgag gtcggggaag tgatgagtgt aaaagatctc ttgtactctg tggctgtagg
     1321 ttctgccaac gactctgcag tggctctggc agagttccac agcggatcgg aggccgcttt
     1381 tgtggagaag atgaacgcta aagcggcaga actcgggctg accaataccc attttgtcaa
     1441 cagcaatgga ctgcctgaaa gcagccacag ggccattgac ggagatcatt actccagtgc
     1501 cagagatatc gcaaaactca ccgttgaagc ttataagtat cctctttttg ccgagatggt
     1561 gtcaacgtac gagtacacag ttaggccagg acaaaaggga gaagtggttc tctacaacta
     1621 caacaagatg tttctcaggg gttaccccgg agccgatggc gtgaaaaccg gcatgattga
     1681 agctgctggt tactgcttga gtggttctgc ggtgcgagac ggcctcaggc tcatcgccgc
     1741 cgtcctagga gccaaatcaa acaacgatcg ctatcgggat atggtaagca tcctggatta
     1801 cggctacaac aactacaagt ccctggttct ggcaaatgcc aaggaacaga tagccgagat
     1861 caaagtcagc cacggacggc gcgaggtctt ggagctcttg gcaaagcagg acttgagggt
     1921 aacggtgaaa aaagtcgggg acgagacgcc aaccctggac gttcatgttc ctgaggtggt
     1981 gaaagctccg atcacagagg gtgaaagcct tggagtaatc tacgccaagc tcggtggtga
     2041 agttgttggc gaagtggaac tggttgccgc aaggggcatc gagaaagccg gcctcgtcct
     2101 ggaaatatcg agaagttttt tgcaactttt gggaattaat aaattataag gaaggaaaaa
     2161 tcaccccctg gaaagaaagt ttgtctcaag tagcccaggg ggtgatttta tgcctgttag
     2221 tgttagaaag atcggtacga tgttggttgc agaggtggtt ggggatctgg acatggttac
     2281 cggtgcccag ctcaaagagg cagcagatca gatgtgggag cactacgaga cgctgagtgt
     2341 ttttgtgctg gacttgagca aagtgggttt taacgacagc tcgggcctcg gtgctatttt
     2401 aggccggtac aagaaggtga acgccagaag tggtacaatg attattgtag gcgcccaagg
     2461 cagcgttagg gaggacttgg agatctcagg aatctccaaa ctctgcttgt ttaaagagag
     2521 cctcaaagag gtggctccag cgcaggagta gcctgccctg ccttttgatt attgcgaggg
     2581 gaggacggca ctgtctaagc gtgccaaatg tctatgacac agaagaatga agttcatttt
     2641 cagttcccga gtttgcctgt gaatatcgct tttgcccgat ccacggtagc ggcatttgcc
     2701 gtccagatcg agcagtttac agaggccgaa tacaacgaaa tccgcaaagc cgtgtccgag
     2761 gcagtctcga actgtgttca gcatgcatac cccgattcgg tgggaacggt tagtatcagc
     2821 gtgtatatcg aaggcgacct tttgacaata gttattgaag accaaggcgt tggcattgaa
     2881 aatgtcgaat tggctaagac aaccgagttt acaacaaaac ctgatgagaa tcttggctgg
     2941 ggttttaact tcatggaagc ctgtatggat gagatctctg tggaatctta tccgggaaaa
     3001 ggcacaaaac taactatgaa aaagagaccg catgcaaacg atgcccaatg atatcgaact
     3061 actccgccaa gcgaaagcag gtagcaaaga agcgagagat agattcctgg aaaacaacac
     3121 ggcactagtg gtacatgtgg ccaagaggtt ttctgcaggc gaatacgagg atctggtaca
     3181 agccgggttt gttgggctca ttaaagcact tgacagcttc gatcctggtt ttggcactgc
     3241 gttttccaca tacgcagtac cctacattat gggag
//
LOCUS       DAOSDA010000188         3256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_18874_length_3256_cov_3.939706, whole genome shotgun sequence.
ACCESSION   DAOSDA010000188 DAOSDA010000000
VERSION     DAOSDA010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18874_length_3256_cov_3.939706"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLW82_05185"
     CDS             <1..429
                     /locus_tag="PLW82_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ46700.1"
                     /translation="VEDAAKKAQIFDAIMEFPEQFETKVGERGVTLSGGQKQRVSIAR
                     ALLKRSPVLILDDSLSAVDTKTESLILKNLQEATSSDQQTVIIISHRISTIENCDDII
                     VLDEGRIIERGTHDELVRQNGFYAELYRKQLLEEQIQQFA"
     gene            488..2479
                     /locus_tag="PLW82_05190"
     CDS             488..2479
                     /locus_tag="PLW82_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46701.1"
                     /translation="MKRLLQYAVPHAGKLIAALLLLALITLGDLAGPYLMKVIIDDHL
                     DPSNSPYVAIPIEEVENYQGQGIDGMAFIRKSEEHSGLQEYYLLSQGGSFYFAPFKVT
                     GAYTIKENTLTVNGQSYDVIYVPKAEAKVIRSSDYNSVMKLSAVYLVLMVGLGLLTYV
                     QGYILTWGGQAIIYAIRQEIFEHLQHLDLAYFDKNYVGRIVTRATNDVENLNEMYTDI
                     LVNTIKDVLTLIGIVVIMLRLDWKLSLITFTVVPLMIAGTIVFRKKVREAYRKVRRYL
                     SELNGFLAESISGMRIVQIFNQEKRKYKEFLKINKDYETSSLGEITVYAVFRPFMDLL
                     YYVALVLILFYGGGQVLQGRVQFGLLIAFISYAQRFFGPINALTEKFNIMQSAMASAE
                     RIFQLLDTKPTIVQPKDPKTPTEVKGGIEFKNVWFAYNGDEYVLKDISFVANPGETVA
                     FVGHTGAGKTSIINLVNRFYDIQKGQILIDGLEIREWDLTELRKRIGIVLQDVFLFSG
                     DIKSNIRLNNEDISQKDILEACRAVGADKFIMKLPRGYDEKVTERGSTLSQGQRQLLA
                     FARALAFDPDILILDEATSNIDTETELLIQEALKRVTKDRTTLVIAHRLSTIQHADKI
                     IVLHKGEVAEMGTHQELLARGGLYYKLYQLQYMSQKNLA"
     gene            2692..>3256
                     /locus_tag="PLW82_05195"
     CDS             2692..>3256
                     /locus_tag="PLW82_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46702.1"
                     /translation="MLFVVLALLPTVLVWSMVAELNRQTDRVLPAIRHVETLNTKFSG
                     ADRIRLEGFIGDIEVVYDDTHTIFIEGEKYIAHTKLDMAAFESTSIEYRQEGDVAVLK
                     WAPPKNFGGTVGFNGKIVLPKKVHELDVEVREGSLSVTGGISSLIITTGTTDVTVNDH
                     EGNLVVDVLEGNVTLKNIKPDQVLKVNV"
BASE COUNT          904 a          737 c          826 g          789 t
ORIGIN      
        1 gtcgaggatg cggcaaagaa ggcgcagatc tttgatgcga tcatggagtt tcccgaacag
       61 tttgaaacta aagtgggaga gagaggggtt actctttcag gaggtcagaa acagagggtg
      121 tctattgcca gagctctgct caaacgttct cctgttttga ttctcgacga tagcctctct
      181 gctgtagata cgaaaaccga gtctctgatt ctgaaaaacc tccaagaggc cacaagctcc
      241 gatcagcaga cggtgatcat tatctcccac agaatctcca ctattgaaaa ctgcgacgac
      301 atcatcgttt tggacgaggg aaggatcatc gaaagaggta cccacgatga gttagtcaga
      361 caaaacggct tctacgcaga actctacaga aagcagctgc tcgaagagca gattcagcag
      421 ttcgcataag ggggtgttag atgtggctga tgaagagatt ttgggaaaag catacgacag
      481 tagattaatg aagcggctct tgcaatacgc agtaccccat gccggtaaac tcattgcggc
      541 actgctgcta cttgcactaa tcactttagg agaccttgca ggaccttatc tgatgaaagt
      601 catcatcgac gaccatctag acccgtccaa cagtccctat gtggctatac ctatagaaga
      661 agtagagaac taccaaggtc aaggcataga tggcatggcc ttcatccgaa agtccgaaga
      721 gcattcaggc ctccaggagt attaccttct cagtcaagga ggcagctttt actttgcccc
      781 cttcaaggta acaggtgctt acacgatcaa ggagaacact ctgactgtaa atggccagtc
      841 ctatgacgtg atctatgtgc ctaaagcaga ggccaaggtc atcaggagca gcgattacaa
      901 ctcggttatg aagctgagcg ctgtgtatct cgttctgatg gtgggattgg ggctcctcac
      961 ctacgtgcag ggctacattt tgacctgggg aggtcaggcg attatctatg ccatcagaca
     1021 ggagatcttt gagcacctgc agcacttgga tctggcatac ttcgacaaga actacgtagg
     1081 tagaatcgtg accagggcta ccaacgatgt tgaaaacctc aacgaaatgt acaccgacat
     1141 tctggttaac accatcaaag acgtgctcac ccttatcggg attgtggtga tcatgctcag
     1201 gctcgactgg aagctgtctc tgatcacctt cactgtagta cctctgatga ttgcaggaac
     1261 catcgtgttc agaaagaaag ttagagaagc ctacagaaaa gtgagaagat acctttcgga
     1321 actcaacggc ttcctcgctg aatccatttc cggcatgagg atcgtgcaga tctttaacca
     1381 agagaaaagg aagtacaaag agttcctgaa gatcaacaag gactatgaga cctcatctct
     1441 tggtgaaata actgtttatg ccgtctttag gcctttcatg gatcttctct attacgttgc
     1501 ccttgtactt attttgttct acggcggggg acaggtgcta cagggcagag tccagttcgg
     1561 tcttctgatc gcatttatca gctatgccca gaggtttttc gggccgatca atgccctaac
     1621 agagaagttc aacatcatgc agtcagccat ggcttctgca gagaggatct tccaactcct
     1681 ggacaccaaa cctacgatcg tccagccgaa agatccgaaa actcctactg aggtcaaagg
     1741 cggaattgag ttcaaaaacg tctggtttgc ctacaacggt gacgaatacg ttttgaagga
     1801 tatttcgttc gtggcaaatc ccggtgagac tgttgctttt gtgggtcata ctggagcggg
     1861 gaagacgtcc attatcaatc tggtgaaccg cttctacgac atccagaagg gacagattct
     1921 catcgatggg ttggagatca gggagtggga tctcaccgaa ctgagaaaga gaatcggtat
     1981 cgtcctacag gatgtattcc tgttttccgg agacatcaag agcaacatca ggctgaacaa
     2041 cgaagacatt agtcagaaag acattcttga agcctgcagg gctgtaggtg ccgataagtt
     2101 tatcatgaag cttccaaggg gctacgatga aaaagtaacg gaaagaggtt caaccctgtc
     2161 acaagggcag agacagcttt tggcgtttgc cagggctttg gctttcgatc cggatatctt
     2221 gattcttgac gaagcaacct ccaacatcga taccgaaaca gaacttctga tccaagaagc
     2281 tcttaagagg gtaaccaagg acaggacgac cttggtcatc gcccaccggc tttctacgat
     2341 ccagcatgca gacaagatca tcgtgcttca caaaggcgag gtagcagaga tggggaccca
     2401 ccaagaacta cttgctcgtg gaggattgta ctataagctg taccagctgc agtacatgtc
     2461 ccagaaaaat ctcgcgtaaa aacattaagg agggaagacg gtttgagctt gctgcttttc
     2521 ccttatgttc agcctagcga aaagtgccga gactacgtgg cttaacctcc atgtgaggtt
     2581 gtccagtcgt ttttcgggcc taagctgcac tctcaaacct tcaccttgcc ctccaaacac
     2641 tttagtctca gtttgtgact caggaaagga gagcagtagt ggtgaaaaaa agtgttgttt
     2701 gttgtgttgg cgctccttcc cactgttttg gtgtggagca tggttgctga gttgaacaga
     2761 cagacagatc gggttttgcc agccatacgg cacgtggaaa ccctaaacac caagttctcc
     2821 ggggctgata gaatacgtct ggaaggcttt attggtgata tcgaagttgt ctacgacgac
     2881 actcacacta tctttatcga aggtgagaag tacatcgctc acacaaaact cgacatggca
     2941 gccttcgaaa gcacttctat tgagtacaga caggaaggag acgtcgctgt acttaagtgg
     3001 gcgcccccga aaaactttgg cggcacggtg ggttttaatg ggaagatcgt tttgcccaaa
     3061 aaggtacacg agcttgatgt ggaagtccgt gagggtagtc tgtctgttac tggaggaatt
     3121 agcagcttga tcatcaccac aggcacgaca gatgtaacgg taaacgatca tgagggcaac
     3181 ctggtggttg atgtacttga aggaaacgtg acgttgaaaa acatcaaacc agatcaagta
     3241 ctaaaggtta atgtgg
//
LOCUS       DAOSDA010000189         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_18894_length_3254_cov_2.972179, whole genome shotgun sequence.
ACCESSION   DAOSDA010000189 DAOSDA010000000
VERSION     DAOSDA010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18894_length_3254_cov_2.972179"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLW82_05200"
     CDS             complement(<1..387)
                     /locus_tag="PLW82_05200"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOJ46703.1"
                     /translation="MNQIDDALLIRQAKKGNQEAINSLVEKYADYTQKSVLKILHKMS
                     RQGSYISDTYRSTGDIISDIEEDSWAIAHDVLIKAMRSIKNFEGRSKFTTWLWVIAEN
                     HVKTLMAQRRKKRDRLFLQHSENDEVA"
     gene            804..1376
                     /locus_tag="PLW82_05205"
     CDS             804..1376
                     /locus_tag="PLW82_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOJ46704.1"
                     /translation="MKAFWESFSLVFVAETGDKSQLLVLALAAKFPLLPLIAGVTSSI
                     IVSQLVATSIGFLLGEAMPVNVLNFLGGLLFIAIGLKTIISPEVAKERQDKVECPAKP
                     FLKVFTLYTIGELGDKTQITCMLLASQNNNFFGTFSGATLAMMLANSLGLVFGKFVSY
                     QLPEKALKAVAAVTYIVFGILRLVDVLGRI"
     gene            complement(1475..>3254)
                     /locus_tag="PLW82_05210"
     CDS             complement(1475..>3254)
                     /locus_tag="PLW82_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOJ46705.1"
                     /translation="VSTYLTFLATKAPFSTPALFLIPLVVVVVAYLFRLYRVIYRHLD
                     FIDIWKMAWAVLLSHVITYVVFKNLGIRFAFTSLAVFLCALCGSRVAFKIWTSKRIKD
                     KHPSHQQKLRTVIIGAGQAGILVSAEIEKHTNLRADVIGFVDDDLTKLGRTIHNIPVL
                     GTLDDLERIIGQYRIEQVIFAIPSSSGALVRKVNSVVKKYKVKFKILPGVFEILDGSV
                     QVSTVCDVSIDDLLRREPIKIDIDTISSDLTGKVVLVTGAAGSIGSELVRQICKFKPK
                     EIVLLDINENGLHDMHLELKNKHPEQAHRCVVANIREQKRIDELFEEIRPSIVFHAAA
                     HKHVPMMEYNPGEAIKNNVFGTLNVALAASKYNAKRFVLISTDKAVNPTNVMGATKRT
                     AEKIIQSLGQKSSTIFTAVRFGNVLGSNGSVVPIFKQQIASGGPVTVTHPDIKRYFMT
                     IPEACQLVLQAGINAEQGEVYILDMGEPVKIVDLAKAMIELSGYTVGEDIEIKFVGLR
                     PGEKLFEELFADTESSSPTSHNKVMRAHLGKVDGDTLLRQLEELEEVAKSGDHVAMRQ
                     ALQKIVPNYSVDYDKVLEEIAATRNY"
BASE COUNT          790 a          801 c          698 g          965 t
ORIGIN      
        1 ggcaacttcg tcgttttcgg agtgttgtag aaataaccga tcccgttttt tccttcgttg
       61 agccattaac gttttgacgt ggttctccgc gatgacccat agccatgtag tgaattttga
      121 gcgtccctca aaattcttta tggatcgcat agctttaatc aaaacatcat gagcgatagc
      181 ccatgagtct tcctcaatat cagagatgat gtcaccagtt gatcgatacg tgtctgatat
      241 gtagctgcct tgtctcgaca tcttgtgcag gatcttcaaa actgattttt gagtataatc
      301 ggcgtatttt tcaactaaac tgttaatggc ttcttgattg ccttttttgg cctgccgaat
      361 tagtagtgca tcatctattt ggttcatgtt atttctatcc ttacatggcc caaggatatc
      421 cactccttta cttatgttca aattaaataa ctccgagcgg atatccttct tcgcaaacta
      481 ctttgactca ctactttagt tctggttacc tacctttatg taacaagcgg tagcagaccg
      541 caatacccac tggtaaaacc ttctattagc aaggtttgca cgcgttgcgc agggtctaga
      601 gaacatgttc ttcctcgaac acctgtcatc gtattaacac aatgtttagt tcaacttcgt
      661 gaatatctag ttcgaaagta aacaccactt tgctggcttc tgattaccag catttgccta
      721 aacgcatctc cttagatagc cttcgttttt caccagtaac ttacttatca ttggcatttg
      781 ctgctcgggg gatagtatag acaatgaaag cgttctggga gtccttcagt ttggtctttg
      841 tcgctgaaac aggagacaaa tcgcagctgc tggttctggc gcttgctgcc aagttccccc
      901 tccttcccct tatcgctggt gtcacttctt cgatcatcgt ctcgcaactg gttgcaactt
      961 ccatcggttt tctactggga gaagcaatgc ctgtaaacgt gttaaacttt ctcggcggcc
     1021 tgctttttat tgcaattggc ctgaaaacca ttatctcccc cgaagtcgcg aaagaacggc
     1081 aggacaaagt tgagtgtcct gccaaaccgt tcttgaaagt ttttaccctc tacaccatcg
     1141 gcgaactagg cgacaagaca caaatcacct gtatgctcct ggcaagtcaa aacaacaact
     1201 tcttcggcac tttttcaggt gcaactttgg caatgatgct tgccaattct ctggggctgg
     1261 tctttgggaa gttcgtaagc tatcagctgc ctgaaaaagc cctcaaggcc gtagcagcag
     1321 tcacgtatat cgtttttggc atcctgcgtc ttgtcgatgt actcggtcga atttgaacgc
     1381 tcggaacagc gctggcaact atgtaacgca gtatacaaag ggaccttcaa aaagcgaggc
     1441 tcacctctca atttgaaggt cataattgtt aatcttaata atttctggta gccgcgatct
     1501 cctcaagaac tttgtcgtaa tcaacgctgt aattgggaac gatcttctga agtgcttgac
     1561 gcatggcaac atggtcgccg ctttttgcaa cttcttccag ctcttctagc tggcggagca
     1621 aggtgtcccc atcgactttc cctaagtgcg cacgcattac cttgttgtgg ctagttggcg
     1681 acgaagattc ggtgtcggca aaaagctctt caaatagctt ttctccaggc ctgagtccca
     1741 caaacttaat ctcgatgtct tcacctacag tatatcctga aagttcgatc atggcttttg
     1801 ctaagtccac aatcttcaca ggttcaccca tgtctaggat atacacttcg ccttgttcgg
     1861 cgttgattcc cgcttgaaga accaactggc aagcttcggg aatggtcata aagtagcgct
     1921 tgatgtcggg atgcgtgaca gttaccggac cgccacttgc aatctgctgc ttaaatatcg
     1981 gaaccacgct tccgttgctg cccaagacgt tgccaaagcg aactgcggtg aagatcgtcg
     2041 aacttttttg tccgagggat tgaatgatct tctctgcggt gcgctttgtt gctcccatga
     2101 cgttggtcgg gtttacggcc ttgtctgtgg agatgagtac aaaccgtttg gcgttatact
     2161 tagacgctgc caaagctaca ttcaacgtac cgaaaacgtt gtttttgatg gcttctcccg
     2221 gattgtactc catcatcggc acgtgtttat gcgctgcagc gtgaaataca atactcggcc
     2281 tgatctcctc gaaaagctcg tcaatccgtt tctgctccct gatattggca acaacgcagc
     2341 ggtgtgcctg ttcagggtgt ttgttcttca gttccaaatg catgtcgtgg agaccgtttt
     2401 cgttgatgtc cagaagcaca atctctttcg gtttgaactt gcagatctgc cgcacaagtt
     2461 cagaaccgat cgatccagca gctccggtta ctaacaccac tttgccggtt agatcggaag
     2521 agatggtatc aatatcgatt ttaatcggct ctctacgcag aagatcgtcg atgctcacat
     2581 cgcagaccgt gctcacctgg acactgccgt ctaggatttc gaacactccg gggaggatct
     2641 tgaacttcac cttatacttc ttaacaacgc tgttgacctt tctcaccagt gcacctgagc
     2701 tgctgggaat agcgaaaatc acctgttcga ttctgtactg tccaataatc cgctccaaat
     2761 cgtctaacgt acccagtaca ggtatgttat gaattgtccg tcccaatttc gttaggtcgt
     2821 catcaacaaa acctatgacg tctgctcgta ggtttgtgtg tttttcgatt tctgcgctaa
     2881 ctaaaatgcc ggcttgacct gcgccgatga tgactgtcct tagtttttgt tggtgagacg
     2941 gatgcttgtc ctttattctt ttgcttgtcc aaatcttaaa agcaaccctg gacccgcaca
     3001 atgcacacag aaagaccgca aggcttgtga acgcaaatct aattccgagg tttttgaaaa
     3061 ctacgtatgt aataacgtgg ctcaaaagca ctgcccaagc catcttccag atatcaataa
     3121 aatcaagatg cctgtatatt actctgtaca atctgaataa gtacgctaca acaactacaa
     3181 caagtgggat caagaacaac gctggcgtgc tgaaaggagc cttcgttgca agaaacgtaa
     3241 gatatgtgct gacg
//
LOCUS       DAOSDA010000190         3245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_18978_length_3245_cov_2.836677, whole genome shotgun sequence.
ACCESSION   DAOSDA010000190 DAOSDA010000000
VERSION     DAOSDA010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18978_length_3245_cov_2.836677"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PLW82_05215"
     CDS             complement(<1..767)
                     /locus_tag="PLW82_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOJ46706.1"
                     /translation="MKDRTKFLILVLLALLVIGLYLSLGLTPKNYKYALSRRVPKVIA
                     IVATAFSIAVSTGVFQTVTNNNILTPSVMGLDSLYLLIQTLIVFGLGSSSRLVVDSNL
                     NFLLNTGLMILFSVLLFKVLFRGDLGSHNIFRLLLIGMIAGTFFQSLSSFMQMIIDPN
                     EFLVLQGRMFASFNSIDTGILTVSLSIMALVFVVLGFYLRKLDVVALGRDHAINLGVD
                     YDKLVQKMLVLVSVLVSVSTGLVGPITFLGLMVVNVAR"
     gene            complement(764..1720)
                     /locus_tag="PLW82_05220"
     CDS             complement(764..1720)
                     /locus_tag="PLW82_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ46707.1"
                     /translation="MRILKSSYLLVILAVLSVISLFIGVSDLNLISLIRGESSHVQIL
                     VLSRIPRLMAIIITGMGMSVSGLIMQQLSRNKFVAPSTSATIDSAKLGVLVSMVLFPG
                     AKALGKMSIAASFAFLGTFLFMALLKRIKIKNAIFIPLIGIMLGSIIDSVTTFFAYRF
                     DMIQNVSSFLMGNFSMVIQGRYELLYLSIPMMILAMLYAKKFTIAGMGEEFATNLGLN
                     YNTVVNIGLGIVSVISALSIITVGRIPFIGLVVPNIVSLYYGDNLEHSLGTTAVLGSV
                     FLLVCDIIGRVIIYPYEISIGLTVGVIGSFIFLLLLARRKTA"
     gene            1873..2631
                     /locus_tag="PLW82_05225"
     CDS             1873..2631
                     /locus_tag="PLW82_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ46708.1"
                     /translation="MKKLTRLYTVMELFSNCTSASIAAFSKDGEFLLDYGLFKCYLFL
                     RNDKRLMQSIALELAEKISSGEGRTVNEPWVVKKIDDAELAFCLMDPKDKNSGFFLIA
                     PSPKYRNKIPYDLRTTISYLIGLLRIIEKDVSGKKPAVVKTDQVYSFHVQKAVDYIHS
                     FYYEEITLESMASRLGLNKSYFASIFKKETGQTFTQMLQNVRIENSKKSLEDGTGSIL
                     DVALAVGFTSQNYFATVFKKATGMTPRQYRQKLS"
     gene            complement(2730..>3245)
                     /locus_tag="PLW82_05230"
     CDS             complement(2730..>3245)
                     /locus_tag="PLW82_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOJ46709.1"
                     /translation="KTEKNLLEAFSGESQARNKYTYFADVAKREGYEQLAEIFLKTAR
                     NEQEHARLWLQELGLIGSTSENLLAAAEGENYEWTDMYDRFAKEAEEEGFPELAEKFR
                     QVAAIEKAHEERYRKLLRNVEMQQVFAKGEETMWECRVCGHLVMGKKAPEVCPVCLHA
                     QSYFEVRAENY"
BASE COUNT          886 a          822 c          748 g          789 t
ORIGIN      
        1 cgcgctacat tcaccaccat tagccccaag aaagtgatgg gtcccacaag acccgtggac
       61 accgacacca aaaccgatac caaaaccagc atcttctgca ccagcttgtc gtagtctacg
      121 ccgaggttga ttgcgtggtc tctaccgagg gcaactacat ccagctttct caggtagaat
      181 ccgagaacta caaacaccaa ggccatgatg gaaagggata ccgttaggat acccgtatcg
      241 atgctgttga agctggcaaa cattctgccc tgcagtacca aaaactcatt gggatcgatg
      301 atcatctgca taaacgaaga caggctctgg aaaaacgtcc cggcgatcat gccaatgaga
      361 agcagtctga agatgttgtg acttcctaaa tcgccccgaa acaacacttt gaaaagaagc
      421 accgaaaaca agatcatcaa accagtgttc aggaggaagt tcaaattgct atccacaacc
      481 agcctgctag aagatcccaa cccaaaaacg atgagagtct ggatcaatag atagagggag
      541 tctaacccca tgacgctggg agtgaggatg ttgttgtttg tgaccgtttg aaaaacaccg
      601 gtagaaactg cgatggaaaa tgccgtagcg acgatagcaa tgactttagg aacccttctg
      661 gaaagagcgt atttgtagtt tttcggcgta aggcctaaag acagatacaa accgatcacc
      721 aacagcgcga gcaaaactaa aatcaagaat tttgtcctgt ccttcatgct gtcttcctcc
      781 ttgccaaaag caaaaggaag ataaagcttc cgattactcc cactgtaaga ccgatggaga
      841 tctcgtaggg atagataatc acacgcccga tgatgtcgca gaccaggaga aagaccgagc
      901 cgagaaccgc tgtggtgccc aggctgtgct ctaagttgtc gccgtagtag aggctgacga
      961 tgtttggcac caccaaaccg atgaacggta tgcggcctac agtgatgatg gaaagggcgg
     1021 atatcacaga cacaataccc agaccgatgt tcaccactgt gttgtagttc agcccgagat
     1081 tcgttgcaaa ctcctcaccc atgccggcga tggtaaactt cttggcataa agcattgcta
     1141 gtatcatcat gggaatgctc aggtaaagga gctcgtagcg tccctgaatg accatggaaa
     1201 agttgcccat gaggaacgat gaaacattct gaatcatgtc gaagcggtaa gcaaagaatg
     1261 tggtcaccga gtcgatgatg cttccgagca taatgccgat tagaggaatg aaaatcgcgt
     1321 ttttgatttt gatccgcttc agtaaagcca tgaacaggaa tgtgccgaga aaggcgaatg
     1381 atgcggcgat ggacatcttc cctaaagcct ttgctccggg aaacagtacc atagaaacta
     1441 aaacccccag ttttgccgag tcaattgttg cagacgttga cggggcaaca aacttgtttc
     1501 tgctcagctg ctgcataatg aggccgctga cgctcattcc cattcccgtg atgatgatgg
     1561 ccatcaacct ggggatgcgg ctaagaacca gtatctgtac gtggctgctc tcacctcgaa
     1621 tcaagctgat caggttcaga tccgatactc cgataaataa tgaaatcact gatagaactg
     1681 caagtattac caacaaatag ctgcttttta aaatccgcac tctattgaca ccggccacct
     1741 ccactgttaa cattaactca tagctttatt gtgaatgatt ctcatttgca atagtatcac
     1801 ggttgtttgg accattcttt caccaggttt aggttttttt cacggtattt agttagattt
     1861 tgaggtgaag ctatgaaaaa gctgactagg ctctacacgg ttatggagct attttccaac
     1921 tgcacaagcg catccattgc tgcattttcc aaagacggcg agtttctttt ggattacggc
     1981 ctttttaagt gctatctctt tctgagaaac gataagcgtc tgatgcagag catcgctttg
     2041 gagctagcgg aaaaaatctc ctcgggagag ggtcgcaccg ttaacgaacc ctgggtcgtg
     2101 aagaaaatcg acgatgcaga acttgccttc tgcctgatgg accccaagga taaaaacagc
     2161 ggcttcttcc tcatcgcacc ctctccgaag tacagaaaca aaatccccta cgatctaagg
     2221 acaaccatct cctacctcat cgggcttctc cgcatcattg aaaaggacgt ctccgggaaa
     2281 aaacccgctg tggtaaaaac cgatcaggtc tacagcttcc acgtgcaaaa agccgtggac
     2341 tacattcatt ctttctacta tgaggagatc accctcgaat caatggcaag ccggctgggc
     2401 ttgaacaaaa gctactttgc ctcgatcttc aaaaaagaga caggtcaaac tttcacccaa
     2461 atgctgcaga acgtgcgcat tgaaaacagc aaaaagtccc ttgaggacgg caccggctcc
     2521 atcctggacg tagcccttgc tgtgggtttt accagccaga actactttgc cacagtcttc
     2581 aagaaggcga ccggcatgac tcccaggcag taccggcaga agctgtcctg aggtttgtgt
     2641 tgaacataaa aaagaggcag gaggttccaa taggaacccc tgccttttca tttgcgctta
     2701 ttctttcgcc gctttgcgct ttacacatat tagtagtttt cagcgcgcac ctcaaagtag
     2761 ctttgagcat gaaggcacac cggacagacc tccggggctt ttttgcccat gaccaagtga
     2821 ccgcagactc tgcactccca catggtctct tcgcctttgg caaaaacctg ctgcatctct
     2881 acgttgcgta ggagcttgcg gtacctttct tcgtgggctt tttcgatagc tgcaacttga
     2941 cggaacttct ctgcaagttc gggaaaccct tcttcttccg cttctttggc aaaacggtcg
     3001 tacatatcgg tccattcgta gttctcgcct tctgcagcag caagcaggtt ctcgctggtg
     3061 gagcctataa gacctagctc ctgcaaccac aggcgggcat gctcctgttc gtttcttgcg
     3121 gtcttcagga agatctccgc aagctgctcg tagccctctc tcttggctac atcggcgaag
     3181 taagtatact tatttctggc ctggctttct ccggaaaaag cctccaagag gtttttctcg
     3241 gtctt
//
LOCUS       DAOSDA010000191         3242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_19000_length_3242_cov_2.883590, whole genome shotgun sequence.
ACCESSION   DAOSDA010000191 DAOSDA010000000
VERSION     DAOSDA010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19000_length_3242_cov_2.883590"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PLW82_05235"
     CDS             complement(<1..593)
                     /locus_tag="PLW82_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ46710.1"
                     /translation="MVNHAPKSKFASSLRRLALEITALEPLRIGSASNLFEANPNLYP
                     IRLEAHRMLIERVVIDENEPREHLERRLKEELSNVFAELRKKGRKINLDEQRLLEKKL
                     LDDVLGLGEIEELVRDQTVTEIMVNGPNQIYVERNGLLELTDHKFTSNDEVKRIIERI
                     VSPLGRRIDESSPAVDARLPDGSRVHAIIPPLAINGPT"
     assembly_gap    658..667
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<668..1137)
                     /locus_tag="PLW82_05240"
     CDS             complement(<668..1137)
                     /locus_tag="PLW82_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46711.1"
                     /translation="MLISIASLCGGTGKTTVGTNLCELLGASGELEDGVVFIRSNSVI
                     KDSGSYLKSARFEYLCGGMGRHWQKTIDDKTQEKHFVIVETDGRNQWEKEVLLESDMV
                     LLVMTATVEHLQGSMELIRFLFRHSYPKDRIKVVCNRYKLKKGLESDEILAVLP"
     gene            complement(1139..3037)
                     /locus_tag="PLW82_05245"
     CDS             complement(1139..3037)
                     /locus_tag="PLW82_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HOJ46712.1"
                     /translation="MVRHRLTGIATIIMFFSLICLGSYSIELGQIIFIPQTDITRVIV
                     GNSDVVSAVPVKDGVVITAKNVGSSEVMIFGEKGTTTESILVRVPSREKEKALTILEN
                     LGLVLEKDGGHYVIKGRVPDQRTFSYVEGLAAIIDSEVRLDLAVDQDLAQSELEGLLD
                     GLGIKLNMISQTAVLTGSVPSKDLEEGILILVSSVYPEAVSLLEIKEEVQVADLPKLK
                     YKGLKVTDYGEVVILEGKAASEEEKNALGQIFEAYRQNVVNMLVVESKEAPLFQSLFA
                     DLGSLTLERVGGTVVLSGTTDTLAGFNLAADLFNCLYPDGVMKVQISSAERVLTEWAQ
                     ILDLELVAVGEMYAIKGQGEESQVLVEIGRMYGKSVFVIRKEENALEFLRALLEIEDV
                     EMQTFRDTLYVRGLPKEVRGDFELAANALFPKVVFLSEEVNPKDPYTVQVSGFYITEK
                     DLDQISLNAAKLTQGWQEVVLSWEEIGIIKELAKEWEIKQPSVLTYPGESTSIHTGGE
                     IPIPFGDGVEWRQFGVSLDCQFGFAENGLIPGYLNIVVSDLDWGNGVTVQGTSLPALK
                     SYSYAGNVELPKGGGVLLLRYWSSKEGWIKKSLPVLESLPVLGRFLFGTRSQQAQSQI
                     MCVLVEVN"
     gene            complement(3019..>3242)
                     /locus_tag="PLW82_05250"
     CDS             complement(3019..>3242)
                     /locus_tag="PLW82_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46713.1"
                     /translation="DTSLILVGTTEKQAQAISLLKPSAVLTVVPSSGGLKVPADPLVA
                     AKNFLKEENSSTRRVEVIEGGEVVWYDID"
BASE COUNT          725 a          920 c          740 g          847 t
ORIGIN      
        1 gtcggaccgt tgatggcaag aggcggaatg atggcgtgaa ccctcgagcc atcgggcagc
       61 ctggcatcta ctgctggaga gctttcgtcg attcgcctgc ccaaaggcga cacgattctt
      121 tcgatgatcc gcttcacctc atcgttgctg gtgaacttgt gatcggtaag ctccaaaagg
      181 ccgtttcgct ccacataaat ctgatttggt ccgttcacca tgatttcggt taccgtctga
      241 tcacgcacca gctcttcgat ttcgccaaga cccaaaacgt cgtcaaggag ctttttttcc
      301 aagagacgct gctcgtccag attgatcttc cggccctttt ttctcagttc cgcaaaaaca
      361 ttactcagtt cttctttgag ccgccgttcc aagtgctctc ttggttcgtt ttcgtcaatt
      421 accacccgct cgatgagcat tctgtgtgct tcaagcctga tgggatagag gttgggattg
      481 gcttcgaaca agttgctggc gctgcctatg cggagtggct ccaaagcggt gatctccaga
      541 gcgagtcgcc ttagggaaga ggcaaatttg gacttgggag cgtggtttac caagagctct
      601 cctttttttt ctgcctcaag aaggtccggt tcttccggaa gccactgcca tttggagnnn
      661 nnnnnnnatg ggaagaactg ctaggatctc atcgctttct aatcccttct tcaacttgta
      721 gcgattgcat accaccttaa tccggtcctt cggatagctg tgtctgaaga gaaaccggat
      781 cagctccata ctgccctgga ggtgctccac agttgccgtc atcaccaaaa gcaccatgtc
      841 gctttcaaga aggacttctt tttcccactg gttccttccg tctgtctcca caattacaaa
      901 gtgcttttcc tgagttttgt catctatcgt cttctgccaa tgccttccca tgccaccgca
      961 gagatactcg aaacgggcac ttttcaggta acttccgctg tccttaatca ccgagttcga
     1021 acggataaac acaactccgt cttctaactc tccacttgct ccgaggagct cacagaggtt
     1081 ggtgcccact gtggttttcc cggtgcctcc gcagagactg gctatagata tgagcaagtc
     1141 agttcacctc caccaaaacg cacatgatct gggattgagc ctgttgggat ctggtgccga
     1201 aaaggaatct gccaaggaca ggtagactct ccaaaaccgg cagggacttt ttgatccagc
     1261 cttctttgct ggaccagtat ctgagcagaa ggacacctcc gcctttaggc agctccacat
     1321 tgcccgcata ggagtagctt ttcagtgcag ggagagatgt gccctgaacc gtaactccgt
     1381 ttccccagtc taagtcggag actacgatgt tcaggtagcc tggaatcagc ccgttttctg
     1441 caaaaccgaa ctggcaatct agactcaccc cgaattgacg ccactctacc ccgtctccga
     1501 agggaatggg aatctctcca ccggtgtgta tcgacgtgct ttctccagga taggtgagga
     1561 ccgaaggctg tttgatctcc cactcctttg ccagttcctt gattataccg atctcctccc
     1621 agctcaagac cacttcctgc cacccctgag tgagttttgc cgcattgagg gaaatttgat
     1681 ctagatcctt ttcggtgatg tagaagcccg acacctgtac ggtgtaggga tctttggggt
     1741 tgacctcctc gctgagaaaa accaccttgg gaaagagggc gtttgcggca agttcaaaat
     1801 cgcccctaac ctctttaggc agcccgcgaa catacaaggt gtctctgaag gtttgcatct
     1861 ctacatcttc gatttccaga agggctctaa gaaactctag tgcgttttcc tccttccgaa
     1921 tgacgaaaac gctttttccg tacatcctgc cgatctcgac tagcacttgg gactcttcgc
     1981 cttggccctt aatggcatac atctcgccaa ccgctaccag ttccaggtct aatatctgtg
     2041 cccattctgt gagaacgcgc tctgcagagg agatctgcac cttcattacg ccatccggat
     2101 acagacagtt gaacagatca gctgctaggt tgaacccagc aagggtgtcg gtggtgcccg
     2161 agagcaccac tgtacctccc actctctcta gggtgaggct gccgaggtcc gcaaacaaac
     2221 tctgaaacag aggggcttct ttggactcca caactaacat gttgactacg ttctgccggt
     2281 aggcctcgaa gatctgtccg agagcgttct tctcctcctc gcttgcagcc ttgccttcca
     2341 aaatcaccac ctcaccgtag tcggtgacct tcaaaccttt gtacttcagc ttggggagat
     2401 cagcaacttg aacctcctcc ttgatctcca agaggctcac cgcctcaggg tagaccgaac
     2461 ttacgagaat taagatgcct tcttcgaggt cttttgaagg aaccgacccg gtgaggaccg
     2521 ccgtctggct gatcatgttc agcttgatgc ccagcccatc taaaagccct tcaagctcgc
     2581 tctgggctaa atcctggtcc acagcaagat ccaaccgcac ttcgctgtcg atgatcgctg
     2641 caagaccttc tacatagctg aacgtcctct ggtcgggcac ccgcccttta atgacgtagt
     2701 gcccgccgtc tttctccaaa accagaccga ggttttccaa aatggtcaga gctttctctt
     2761 tctccctgct gggcacccgc accagaatgg actcggtggt agtgcctttt tcaccaaaga
     2821 tcattacctc agatgaacca acgtttttcg cagtaatcac aacaccgtct tttacaggca
     2881 ccgcactcac aacatcgctg ttgccaacaa tcacacgggt gatgtctgtt tggggaataa
     2941 aaatgatctg ccccaactcg atgctgtaag atccaaggca gatcaggctg aaaaacatta
     3001 tgattgtcgc aattcccgtt aatcgatgtc gtaccaaaca acctcaccac cttcaatcac
     3061 ctctactctt cttgtggagc tgttttcttc tttaaggaag ttctttgcag ccactagtgg
     3121 atcggcggga accttcaagc ctcctgagga cggaacaact gtaagcacag cactcggttt
     3181 caacaagctg atggcctgag cttgtttttc tgtagttccc acgagaatta agctggtatc
     3241 gc
//
LOCUS       DAOSDA010000192         3235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_19059_length_3235_cov_2.782390, whole genome shotgun sequence.
ACCESSION   DAOSDA010000192 DAOSDA010000000
VERSION     DAOSDA010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19059_length_3235_cov_2.782390"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PLW82_05255"
     CDS             complement(<1..950)
                     /locus_tag="PLW82_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ46714.1"
                     /translation="MKMHAFHRFVYKALWHVLKPFIVRAFNFKFDVAKPMNHPFMVLA
                     NHVTNYDPLLVGLSFPQHMYFVASEHLFRIKVLSSILKFIVAPIVRVKAKTERHTALS
                     ILRTLKNGYNVCMFPEGSTTWSGETGEITEATAKLVKASGSALITYRLTGGFFTAPRW
                     GKSIRKGAMEGRIVREYSPEELKEMTDEELYTRIKSDLYVNAYEDNAVSKISFRGKDL
                     AEFLETALFVCPNCQGIGTLKSQGDVLSCRCGLKLRYTELGFFESLTDNAPPFTTVLE
                     WDRWQRKFVERQAETYKSLPEDTPITSDRDQKVFRFETRRR"
     gene            1301..3130
                     /gene="typA"
                     /locus_tag="PLW82_05260"
     CDS             1301..3130
                     /gene="typA"
                     /locus_tag="PLW82_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOJ46715.1"
                     /translation="MAVRKNEQIRNVAIIAHVDHGKTTLVDSLLKQAGMFRKNEVVAE
                     RVLDSNDLERERGITILSKNTSVLYEDLKINIVDTPGHADFGGEVERVLRMVDGALLL
                     VDAFEGPMAQTKFVLRKALEAGLKIIVVINKMDRPDARPAEVADQVLDLFIELEADDE
                     QLEFPVVYASAREGRASFSQDSLGDSMRPLFELIRKEIPAPQGSDQEPLQLQITTLDY
                     DDYVGRIGIGRITGGIISAGQTIGLARRDGTLEEIRIGKLWTFEGLKRVEAAEVGPGD
                     IVAISGLGHVEIGETVTDPENPRPLPLLKIDEPTMTMNFMVNDSPFAGEEGKFVTSRH
                     LRERLYKEAETNVSLKVEDGDSPDTFIVSGRGELHLGIVIETMRREGYEMAVSKPKPI
                     FKEIGGTLMEPFEKLFVNVPEIFAGRVIENLGRRKGDLLQMEPVGTANVRLEFLIPAR
                     GLIGFRSEFLTLTKGEGIMHHSFESYRPYKGEIQQRTSGSLVAFKAGEATAYALDAVS
                     DRGELFITPGTKVYAGMIVGQHNKEGDLDINVCKKKHLTNMRAAGSDDAIKLSPPRVF
                     ILEQAMEYIAEDELVEVTPKSIRLRKTILDRNLRAKALKKQAQ"
BASE COUNT          836 a          755 c          851 g          793 t
ORIGIN      
        1 cgccttcggg tttcgaaccg gaagaccttc tggtctctgt ctgaggttat tggcgtatct
       61 tccggaagag acttgtacgt ctctgcttgt ctttctacaa acttcctctg ccaccggtcc
      121 cactcgagga ccgtggtaaa cggtggagcg ttgtctgtta agctctcgaa aaatccgagt
      181 tcggtgtagc ggagcttcaa gccgcacctg caggacagca cgtcgccttg gctctttaag
      241 gtgccgatac cctggcagtt ggggcagaca aacagcgccg tttccaggaa ttctgcaagg
      301 tccttgcccc gaaaagagat ctttgagaca gcattgtctt cgtaggcgtt gacgtagagg
      361 tccgacttta ttcttgtata aagctcttca tcggtcatct ctttcaactc ttccggactg
      421 tactccctaa cgattcggcc ttccatagcc cctttcctga tggattttcc ccagcgtgga
      481 gcggtgaaaa agcctcctgt aagcctgtag gttatcagag cactgccgct ggctttaacc
      541 agtttagcag tggcttcggt gatctctcct gtctcgccgc tccaggtggt ggagccttca
      601 ggaaacatgc agacgttgta gccgttcttt agggttctta gaatgcttaa ggcagtgtgt
      661 ctttcggttt ttgctttgac cctgacaatg ggtgctacaa taaacttcaa aatactgctt
      721 agaaccttga tgcggaaaag atgttcactg gcaacaaaat acatgtgctg cgggaaactc
      781 aatcccacaa gcagggggtc gtagttggtt acgtggttgg caagaaccat gaacggatgg
      841 ttcattggct ttgccacatc aaacttgaag ttgaatgccc taacaataaa cggttttagc
      901 acgtgccaca atgccttata gacgaaacgg tggaaagcgt gcattttcat gacaaaagga
      961 ctcctttatg aaaaaaatga acaaaaactt cagtggttta gtctttgcta atttatacaa
     1021 gacgagccaa agaccttttt tagtttggtt ttctcctacc attttttggc accaaatccc
     1081 tgcggcgcaa gatactgcag tgaaatcgta cacaagggtt tttcgcactg ccccgcgttt
     1141 agccggagtt ttgcttggaa ccatccggtg gtaatgaaac tgtcaggaaa gggtggaagt
     1201 tcaacctgga gaaattgtgc tgcgtctttc cgccgatttg tttttggtgt ataataactt
     1261 tttggacact gttctcagtg gggaaggaaa tggtacaggt atggcagtaa gaaagaacga
     1321 gcagattagg aatgtggcga ttatcgccca cgtcgatcac ggcaaaacga ctctcgttga
     1381 tagcctgctc aaacaggccg gaatgtttcg gaaaaacgag gtggtagcgg agagggtttt
     1441 ggattccaac gatcttgagc gcgagcgcgg aattaccatt ctttccaaaa acacctctgt
     1501 gttatatgaa gatttaaaaa tcaacattgt ggacactccc ggacacgctg attttggcgg
     1561 agaagtggag cgggttctca gaatggtgga cggagctctg cttcttgtgg atgcttttga
     1621 aggtcccatg gcacagacca aattcgtcct ccgcaaagct ctcgaagccg gtctcaagat
     1681 catcgttgtg ataaacaaga tggacagacc cgatgcaaga cctgcagaag tcgcggatca
     1741 ggttctcgac ctttttatcg aacttgaggc agacgacgag cagttggagt ttccggtggt
     1801 ttacgcttcg gccagagagg gaagggcaag cttttcgcaa gacagtctgg gagatagcat
     1861 gcgtcctctc tttgaactga tccgcaaaga gattcccgca ccgcagggtt cggatcaaga
     1921 gccccttcag cttcagataa ccaccctgga ctacgacgat tatgtcggca gaataggcat
     1981 cggcagaatc accggcggca tcatctctgc aggccaaacc atcggccttg ccagaagaga
     2041 tgggaccttg gaggagatca gaatcggcaa gctctggacc tttgaagggc tcaaaagagt
     2101 tgaagcagca gaagtaggtc ccggtgatat tgttgccatt tcgggtcttg gacacgtgga
     2161 aatcggcgag accgtaacag accccgaaaa cccgaggcct ctccctcttt tgaaaatcga
     2221 cgaacctaca atgacaatga acttcatggt taacgacagc ccctttgcag gcgaagaagg
     2281 gaagtttgta acctccaggc atctgagaga acgcctgtac aaagaagccg agaccaacgt
     2341 cagcctcaaa gtggaagacg gagactctcc ggacacattt atcgtttcag gtagaggcga
     2401 gctgcacctt gggattgtca tcgagactat gcggcgggaa ggctatgaga tggccgtgtc
     2461 caagccgaaa ccgatcttca aggaaatcgg tggaaccctg atggaaccat tcgagaagct
     2521 gtttgtaaat gtgcctgaga tatttgccgg aagggttatt gagaacttag gccgcagaaa
     2581 aggcgatcta ctgcagatgg agcctgtggg aactgcaaat gtgaggttag agtttttaat
     2641 tcccgcacgg ggactcatcg gttttcgcag cgagtttcta accttgacaa aaggcgaggg
     2701 tattatgcac cactcctttg aaagctaccg tccgtacaaa ggtgaaatcc agcagcgtac
     2761 ctctggctct ctggttgcct ttaaagcagg ggaagcaact gcatatgcgc tagatgccgt
     2821 aagcgataga ggcgaactgt ttatcacccc gggtacgaaa gtctacgccg gaatgatcgt
     2881 cgggcagcac aataaagaag gcgatctgga catcaacgtc tgcaagaaga agcatctcac
     2941 caacatgcgg gcagccggct ccgacgatgc catcaagctt tcgcctccga gggtcttcat
     3001 cttggagcaa gcgatggagt acatcgccga ggacgagctg gtagaagtca cgcctaagtc
     3061 cattaggttg cgaaagacta ttctagaccg caacctaagg gctaaagctt tgaaaaaaca
     3121 agcccagtag aggtttgcat aactattggt aatcgaggtt aacctactct tgactaggtt
     3181 tatacagtca aggaggtaac ttatgaaaag cgttatcggt acctatggtg accgc
//
LOCUS       DAOSDA010000193         3220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_19193_length_3220_cov_2.783254, whole genome shotgun sequence.
ACCESSION   DAOSDA010000193 DAOSDA010000000
VERSION     DAOSDA010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19193_length_3220_cov_2.783254"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PLW82_05265"
     CDS             <1..833
                     /locus_tag="PLW82_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOJ46716.1"
                     /translation="NSSGYSLLDLFIGSEGTLGVVTKITVKLLPLPKKTATLLVPFPS
                     LKAAISTVSLILKSRVLPNALEFMEREIIEAAETYLGKPFPDKSAPAYLLVRFDGNTK
                     EELEQDCDHVADICLAAGAIDAYIASDKEQQQLLWDARGAFLEALKAMSELDEVDIVV
                     PKTEIASLVSYTKDLQQEYGVRILSFGHAGDGNVHIYILKDDFEEEKWQALLPKVMQK
                     LYQKGEELQGQVSGEHGIGYLKKPYLKESLGDTLIDLQQRIKEAFDPHLILNPGKVVS
                     "
     gene            1165..3087
                     /locus_tag="PLW82_05270"
     CDS             1165..3087
                     /locus_tag="PLW82_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOJ46717.1"
                     /translation="MSLSIGIDIGSTTLKIAGIDSGGNLMPIEIKYLLTSERLHESIL
                     SAYEAYIQKHGEPTYLGVTGSGQKLGSLVLGTNCTATEIIAHATGCVFLAKKLNLPLP
                     RTIIEIGGQDSKLIKLQDGIPTFFNMNSVCSAGTGEFLRHTIEENALDFAAFNNYVLN
                     ASGAADLEDICTVFARRSFRHAGQNGVPLDQRLKGLAEAIMRNYLRTVARNYGASFKS
                     TLEPPIYFLGGLANSFALAMTLAKLLGQPVIVPPHFQGIGALGMAVIARESAGRVNTE
                     PTNASRSGECSVESAYCTGCTNSCELTVVQRMGSAFYLGGRCEGAGKAGITSSASISI
                     PTVSKRDLPKTRPIPYPKTDHWGIGIDAGSRQVKAALLHGKDVVYLHTETTAPDLYQQ
                     AKTILEVLQSKITDPNAQVYLKTTGSGGRFLSRKLSRHEFMYETEILCHFRAANTLFP
                     DVGTVIDIGGNDSKIIIATGSGLDFAMNDRCAAGTGSFLEQLAKRFGLTLEEAAELAM
                     SSAKPARIGSRCAVLAETDIVHKMRSGAETKDLLFGACFSVAKTFISDTMHGKSLTLP
                     VVFQGGAFLNQALLRAFTTLLDLKEGEYHIAEDRRFVVGAGAIGAAIYALEAKPSCKL
                     EVARTSEQYSLAGGHR"
BASE COUNT          851 a          853 c          819 g          697 t
ORIGIN      
        1 agaactcctc aggatattcg ctcctggacc ttttcatcgg ttcggaaggg acccttggtg
       61 tggtgaccaa gatcaccgtt aaactccttc cactcccgaa aaagaccgca acgctgcttg
      121 tcccctttcc ctccctcaag gcagctatct ccaccgtttc gctgattctt aaaagccggg
      181 tgctgccaaa tgccctggag tttatggaaa gagagatcat cgaagctgca gaaacctacc
      241 tcggtaaacc attccccgat aaaagcgcac ctgcttacct gctggtgcgg tttgacggaa
      301 acaccaaaga ggagttagag caggactgcg accatgtagc ggatatatgc cttgcagctg
      361 gagccatcga cgcctacatt gcaagcgata aagagcagca acagctcctt tgggatgcaa
      421 gaggtgcatt tcttgaggca ctaaaagcaa tgagtgaact cgacgaggtt gatattgttg
      481 tgccaaagac ggagatagca agccttgttt cctacacaaa agaccttcaa caagagtacg
      541 gagtgaggat actcagcttc ggccacgcag gagacggaaa cgtccatatt tatatcttga
      601 aagacgattt tgaagaagaa aagtggcagg ctttactccc aaaggtcatg cagaagctgt
      661 accaaaaggg cgaagagctg caaggtcagg tgtcgggaga gcacggtata ggctatctga
      721 agaaacccta cctcaaagaa agtctcggag atacgcttat tgacctccaa cagaggatca
      781 aagaagcttt cgatccccat ctgatcttga acccaggtaa ggttgtgtct tagctcattt
      841 ggatcgggaa agtcgaaaac cagcatttca atcggcccgg cactctgcgc ggccgtaggc
      901 aaagcggcac caaccgtaaa gtggttggtg ccgcttcatg ttgtcatagc agggcggtgg
      961 tttcgctctt ctatagcgct gaaaacagag ccaaaaaaga aaactgaaca ctcaatccat
     1021 gaaaacccgg tgaatcaaag ctcagtttgc cccgatgatc cctctgactg caccagtttt
     1081 aggagcctgt ggggagggat ctcctctgca tatcctcttc catttcaggc atgctaaaaa
     1141 gaggaatttt ggggggagat tgacttgagc ctaagcatcg gtatcgacat cggttcaact
     1201 accttaaaaa tcgccggaat agactctggt ggaaacctca tgcccattga gatcaagtac
     1261 cttttaacca gcgaaaggct ccatgagtcc attctttcag catatgaggc atacatacaa
     1321 aaacacggtg aaccgactta tctcggcgtc accggatcgg gccagaagct cggcagctta
     1381 gttttaggca caaactgcac cgctacagag atcattgccc acgccacagg ctgcgttttt
     1441 cttgctaaaa agctaaacct gcccctcccg agaacgatca tcgaaatcgg cggtcaggat
     1501 agcaagctca taaagcttca ggatgggatc cctacctttt tcaatatgaa cagcgtctgc
     1561 tctgcaggaa ctggtgagtt tctcaggcac actatcgaag agaacgcctt ggattttgca
     1621 gccttcaaca actacgtact gaacgcctca ggggcagcag atctagaaga tatctgcacg
     1681 gtatttgcta ggcgttcatt taggcacgcg gggcaaaacg gggttccgct ggaccaaagg
     1741 ctaaaaggac tggctgaagc catcatgcgg aactacctcc gcaccgttgc aagaaactac
     1801 ggtgcaagtt tcaagtccac ccttgaacct cccatatact tcctgggcgg cctggcaaac
     1861 agctttgctc tggcaatgac tcttgcaaag ctcctagggc agcctgtcat cgttccgccg
     1921 cacttccagg gtatcggtgc cctcggcatg gccgtcatcg ccagagaaag cgctgggcgt
     1981 gtcaatacgg aacctacaaa tgcctccagg agcggagaat gcagcgtgga gagcgcgtac
     2041 tgtacaggct gcaccaacag ctgcgaactg accgtggtgc agagaatggg tagtgccttt
     2101 taccttggcg gaagatgtga gggtgccgga aaagcaggca tcaccagttc agccagcatc
     2161 tccatcccca cagtttccaa gagggatctt cccaaaacgc ggccgatccc ttatccaaag
     2221 acggaccatt ggggtatcgg gatcgatgca ggctccaggc aggtcaaagc agcgcttctc
     2281 cacgggaaag acgtggtgta cctgcacacc gaaacaacag ctccagatct gtaccagcag
     2341 gcaaagacca tcttggaggt tttgcagtcg aagatcacag atcccaacgc acaggtttac
     2401 ctgaaaacca caggaagcgg cggcaggttc ttgagcagaa aactttcaag acatgagttt
     2461 atgtacgaga ctgagatcct ctgccacttt cgggctgcaa acaccctgtt cccagatgtc
     2521 ggcacggtga ttgacatcgg cggaaacgat agcaaaatca tcatcgccac agggagtgga
     2581 ctggattttg ccatgaacga ccggtgcgca gcaggtaccg gttccttttt agagcagctg
     2641 gcaaagcgct tcgggctaac ccttgaagag gctgcagagc ttgcaatgtc gtcagcaaag
     2701 cccgcccgca ttggctcccg ctgcgccgtc cttgcagaaa cagatatcgt tcacaagatg
     2761 cgctcaggtg cggaaacaaa agacctcctc ttcggagcct gcttctccgt tgccaagacc
     2821 ttcatctccg ataccatgca cggcaaatct ctgactctcc ctgtagtttt ccagggcgga
     2881 gcttttctca accaggcact gctgcgagcc ttcaccaccc ttctggatct caaggaaggc
     2941 gagtaccaca ttgcagaaga caggcgcttt gtggtgggag caggtgctat cggcgccgca
     3001 atctacgctt tagaggcgaa acctagctgc aaactggaag ttgcaaggac cagcgaacaa
     3061 tactctttgg caggtggaca cagatgaaaa tcggcattcc cttagtgttc tcgggaactg
     3121 cgccacaggt aagatgtctg gcggaagtcc tgaaaacctc gggttacacg tctttacttc
     3181 aaaaaccccc actcccggaa tctgggaaaa gggtcttttt
//
LOCUS       DAOSDA010000194         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_19271_length_3210_cov_3.110618, whole genome shotgun sequence.
ACCESSION   DAOSDA010000194 DAOSDA010000000
VERSION     DAOSDA010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19271_length_3210_cov_3.110618"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /gene="steA"
                     /locus_tag="PLW82_05275"
     CDS             <1..568
                     /gene="steA"
                     /locus_tag="PLW82_05275"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HOJ46718.1"
                     /translation="LKAIASFIEDRKPLLVAVDGGADALWELGYKPDMIVGDMDSVSD
                     KVLRLGADIVLHAYPDGRAPGLLRLQDLGLNCTVVPCEGTSEDLAFLILNQLGASIII
                     TVGSHSSMIDFLEKGRKGMASTFLARLRAGDKLVDARGLSVIYRARPKNTYAFLVILA
                     AFIPLVVLILVSPPVASWLKIFLRQIRF"
     gene            589..1071
                     /locus_tag="PLW82_05280"
     CDS             589..1071
                     /locus_tag="PLW82_05280"
                     /inference="COORDINATES: protein motif:HMM:NF022824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HOJ46719.1"
                     /translation="MDLRYHAASLIAVFLALALGLIIGGSLASDQNLVLYQENLISRL
                     EAENMALRNKQASDEAGLLALRQEKTKMESNLNNLAAAYITQLSKEKRIKVDGSDALL
                     GVPLASFEGQDYDYYFPFRDGGELSQKAIADGSATFVEVPKSIKLWELALYLVSTQSE
                     "
     gene            1029..1700
                     /locus_tag="PLW82_05285"
     CDS             1029..1700
                     /locus_tag="PLW82_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ46720.1"
                     /translation="MGAGSLPGFDPKRVAVLIPAYNEEKYLPSTIWGLKSAGFEKIYV
                     ADDASADGTAQVALTSGAVLLSSERNLGKGGILNFALPKVDEEVICLIDADLGESSRE
                     AVKLANAVFQGADLAVARFRSKGGFGLVRRAATFLLFCFTGKLFKAPLSGQRAAKRDV
                     WAKLMPFAPGFSVEVAMLKRACRLGLKVAEVPVEMTDRSYGRGIKGITHRFKQLLAVI
                     RGLTS"
     gene            1697..2455
                     /locus_tag="PLW82_05290"
     CDS             1697..2455
                     /locus_tag="PLW82_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46721.1"
                     /translation="MRELVAVFSGIVFGWVLIPRFLQIDRGVFQVENYRKAQVQNSTG
                     VWLALTSILATMFLVIWYSEVPVLWFYVLSISFLGLLDDLLVEKAKGLMGHVKALLQG
                     KVTTGTLKLVFILLLSLGLALVFKRSHVYLDAVLTAIFANTVNTLDTRPKRAITFYLT
                     ILVITTLFGNTDLFRLYGLAVAGMLFVLYPYEAHEKTMLGDAGSNLLGALLGLSLWQF
                     APILKLVLIAIGVLWQIFADTYSLTKLLDRLKQK"
BASE COUNT          814 a          667 c          857 g          872 t
ORIGIN      
        1 ccttaaagcc attgcttcgt ttatcgaaga caggaagcct cttctagtgg cagttgacgg
       61 aggagcagac gctctgtggg agctgggcta caaacctgac atgatcgttg gcgacatgga
      121 ctctgtatcg gacaaggttc tccgccttgg agctgacatt gtgcttcatg catatccaga
      181 tggcagagca cctggtcttt tgcgtttgca ggacttgggg ctgaactgta ccgttgtgcc
      241 gtgtgagggg accagcgaag atttggcctt cctcatcctc aatcagcttg gagcatccat
      301 tatcatcacg gtaggtagtc atagcagcat gatcgacttt ctggagaagg ggagaaaggg
      361 catggcaagc acctttctcg ctagactcag agctggcgat aagttagtgg atgctcgggg
      421 actgagcgta atctatagag ccagacctaa aaacacctat gcatttttgg tgattctagc
      481 ggcgttcatt ccccttgttg tgctgatttt ggtttcgccg ccggttgcca gttggttaaa
      541 gatcttcttg agacagatca gattctaagg agggagcctt ttgtgttaat ggatctgcgc
      601 tatcacgctg cgtctttaat cgcggttttt ctggctctgg ctttaggact cattatcggc
      661 ggaagtctgg cttccgatca gaatctggtt ctgtaccagg aaaatctcat atcccgtttg
      721 gaagcggaaa acatggctct acgaaacaaa caggcttcag acgaggcagg tcttcttgct
      781 cttcgccagg aaaaaaccaa aatggaatca aacctaaaca accttgcagc ggcttacata
      841 actcagctgt ccaaggaaaa gaggatcaaa gtcgacggca gcgacgctct gctcggagtg
      901 cctcttgcgt cctttgaagg gcaggactac gactactact tccccttcag agacggtgga
      961 gagctgtcgc aaaaggccat tgcagacggg agtgcgactt ttgtggaggt gccaaaaagt
     1021 atcaaattat gggagctggc tctttacctg gtttcgaccc aaagcgagta gcggtgttga
     1081 tacctgccta caacgaggag aaatatttgc caagcacaat atggggtctg aagagcgccg
     1141 gttttgagaa gatttatgta gcagacgatg cgtcagcaga tggcacagcc caggtcgctc
     1201 taacaagcgg tgctgtgctt cttagttccg aaagaaacct gggcaaagga ggaatcttaa
     1261 atttcgctct ccccaaggtg gatgaggaag tgatctgcct aatcgatgcc gatcttggag
     1321 aaagcagcag ggaagcggtg aagctggcca atgcagtttt tcaaggggcc gatcttgctg
     1381 tggcgagatt ccggtcaaaa ggcggctttg gtttggtgag aagagccgct acttttttac
     1441 tcttttgttt tacagggaag ctgttcaaag ctccgctttc tggccagagg gcggcaaaaa
     1501 gagacgtatg ggctaagctg atgccgtttg ctccgggttt ttctgtagaa gttgccatgc
     1561 taaagagggc atgcaggctc ggactaaagg ttgcggaggt gcctgtggag atgaccgatc
     1621 gcagttacgg cagaggtatc aaaggcatta ctcacaggtt caaacagctc cttgctgtga
     1681 ttaggggtct tacatcatga gagaactagt cgctgttttc tcggggattg tcttcggttg
     1741 ggttctgatt cctcgctttc ttcagatcga ccggggagtg ttccaggttg aaaactacag
     1801 gaaagctcag gtgcaaaact ccacaggagt ttggttggca cttacatcaa tcctcgctac
     1861 tatgtttctt gtgatttggt acagcgaagt gcctgtttta tggttttacg tgttgagcat
     1921 ttcctttttg gggcttttag acgatctcct ggtagagaaa gccaaaggct tgatgggtca
     1981 cgtcaaagcg cttttacaag gcaaagtaac aacaggcacc ttaaaattgg tgttcatact
     2041 tctcctctcc ctcggtctgg ctttagtttt caagaggagt cacgtgtatc tagatgcagt
     2101 tctaacggcc atttttgcaa acaccgtgaa caccttggac actcgtccca aaagagcgat
     2161 cactttttat ttgacgatcc ttgtcattac aacgcttttt ggcaatacgg atctgtttcg
     2221 cctgtacggg ttggcagttg ctgggatgct tttcgtgctg tatccgtatg aagcccacga
     2281 gaagaccatg ctaggagatg caggaagcaa tctcttagga gcccttttgg ggctttcact
     2341 gtggcagttt gctccgattt tgaagcttgt acttatcgcc ataggagttc tgtggcagat
     2401 cttcgccgat acctactccc tgacaaagct tttggaccgt ttgaagcaaa agtaaaaaag
     2461 ccagtggctt tggaagaagc aaagcaacgg gctttttcta tttctaggga accgtgaacg
     2521 atttgtagca attgacgtta acggaacaac actcttcgaa gagtcaaaga agggtgggta
     2581 ggaagaaacg catccccact gcagaacact ggagtgtact caattttttt tgttcaatag
     2641 taaattatag aaaaagaaaa acatttgaga aggaaacaac tggcaacaga cagaatgttg
     2701 ttatcaagtc gactaaatgt ggagacaaaa cgttctttga gcacgttttt ttgcggtttg
     2761 tcagcatacg agagaggggt ggttgtcaag actttgttaa agggatgtta aaggtcacaa
     2821 cggtggtcta taatgcacta gacgggaaac gacatgcaga acgacaatca tctcgaggtg
     2881 ctttagagat caccaaagaa ttggcggcaa tgcggtatcg ctaactttca ccatctcagg
     2941 aaagaaacgg attacaaccg agtgacgaga tttcgcttgg gaaaagtcac gctgtttgct
     3001 ttcagaattc gatcggaagt tctctttttt acgaagtctt acatacacaa ggcggttact
     3061 tgagtcttcg ttgatcgaaa gtgaatggct ttttgttagt gagtcaaatg ggtgggacat
     3121 ggttgtgttg gtggctgaca gaatgaaaag ggaggtttct attaagcccc ctaattgatt
     3181 tggacaaata gtgtgtgtct gatagaatca
//
LOCUS       DAOSDA010000195         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_19493_length_3183_cov_3.207801, whole genome shotgun sequence.
ACCESSION   DAOSDA010000195 DAOSDA010000000
VERSION     DAOSDA010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19493_length_3183_cov_3.207801"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /locus_tag="PLW82_05295"
     CDS             complement(<1..1562)
                     /locus_tag="PLW82_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOJ46722.1"
                     /translation="MKRGVIDLAMPNQKIELLAPAGSFESLTAAVQSGADAVYLGGKL
                     FSARASAANFSNAEIEEAVAYCKKHNVKVYVTVNILIKNGELQEAAEFVSFLHEAGVD
                     GVIIQDLGLWLLAEDLDGLERHVSTQMTLHNTPGVELLLQAGASQIVLSRELDLAEVS
                     QIKEATGARLEVFGHGALCFSYSGQCLMSSMIGGRSGNRGRCAQPCRLSYKLVDGSGA
                     TYNTEGDYLLSTKDLRTALDLEKLTGVVDSLKLEGRLKRPEYVAAVTGTYRRLLDGEI
                     DKDEAEAALRSVFNRDFTRGYLFGDRGKSIIGYDQPSSRGLEIGRVVSYTKGSLVIRL
                     SQPLNRGDGIEIVQEGRESKSQGTVVGEIKLRGSKVLSAKPGDEVVIPFRGKAKRGDI
                     VYKTKDREAEGEAKNLYKSERVFRKQPVAFFATAKVGSEFVIQVQNRDVIRGEAFSGH
                     ICERAENRPLDLETLRKQIDRLGNTPFSLEALEAELDGEAMVPFSVINDTRRSALEDL
                     EEKIVQHARRKK"
     gene            complement(1641..2798)
                     /locus_tag="PLW82_05300"
     CDS             complement(1641..2798)
                     /locus_tag="PLW82_05300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOJ46723.1"
                     /translation="MRQMRFVLIIAIFLLVILIMQPPKTDEAVVTVAGLSEVTDISSL
                     FRSKPDVGASAAILFDPKSGTILYAKNADSQRAPASTTKIMTAILTLERCSLGESVTV
                     SRRAATINGSRVGLREGEVYTVEKLLWGLLLLSGNDAAIALAEHIAGSVEEFAKYMNT
                     RAAELGSRQTHFVNPHGLSDPNHYTTVRDLAIMASHALKYPLFQEIVSSKEKEIPWQQ
                     SFAKYLKNTNKLLWIMPDADGVKTGTTNLAGACLVSSATRDDRQLIAVVLNSSNRWVE
                     SARLLEWGFANTLCIKVVGKNETIPDIYVKNAKSYAVGGVMGDVFVTVPKGVKPYVRF
                     VPYDLEAPVFKDQLLGILEAGPVSDPNALKKHPVYALEGVARRRSFFGLGI"
     gene            complement(2977..>3183)
                     /locus_tag="PLW82_05305"
     CDS             complement(2977..>3183)
                     /locus_tag="PLW82_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46724.1"
                     /translation="ESSTKYLEGKTLINTRNGEAGIIRSIAGFKRLLLRREIAIEPGD
                     EFVIVDTREEDVLQTLPYAVVKNQ"
BASE COUNT          692 a          870 c          775 g          846 t
ORIGIN      
        1 atcttctttc gccttgcatg ctgtacgatt ttttcttcca ggtcttctag tgcgcttctt
       61 ctagtgtcgt tgattacgct aaagggcacc atggcctcac cgtccagttc agcctcaagt
      121 gcctctaaac tgaagggggt gttccccaaa cgatctatct gcttcctcag ggtttccaga
      181 tccagcggtc tgttctcagc tctctcgcaa atgtgcccgc tgaaggcttc accccgaatg
      241 acgtcccggt tttggacctg tatgacaaac tcagatccga ccttcgcagt ggcaaagaaa
      301 gccaccggct gcttcctgaa gactctctcg gacttgtaca gattcttcgc ttcgccctcg
      361 gcttcccgat ctttggtctt gtagactatg tcgccgcgtt ttgccttccc cctaaaggga
      421 atgaccacct catctcctgg ctttgccgaa agcaccttac tgcctcggag cttgatctcg
      481 ccaactacgg tcccttggct ttttgactcc cggccttctt gaacgatctc aattccgtct
      541 cctctgttta agggttggga aagtctaatc acaagcgaac ctttggtgta agatacaacc
      601 cggccgatct cgagccctct cgagctcggc tgatcgtatc ctatgatgct ctttccccta
      661 tcgccaaaga ggtaacctct ggtgaagtcc ctgttgaaaa ccgagcgcag ggcagcctct
      721 gcctcgtctt tgtcgatctc gccgtcgagc agcctccggt aggtgccagt aactgccgct
      781 acgtattctg gtcttttcag tcttccttct agtttgaggg agtccacaac tcccgtcagt
      841 ttctccagat ccaaagcggt ccggagatcc ttagtgctga ggaggtagtc tccttctgtg
      901 ttgtaagtag cccctgaacc gtctacaagc ttgtagctga ggcggcaggg ctgagcgcat
      961 ctgcccctgt ttccgctccg tcctccgatc atgctgctca tcaagcattg cccgctgtag
     1021 gagaagcaca gagcgccgtg accgaagact tcaagcctgg caccggttgc ctctttgatc
     1081 tgggaaacct cggccaggtc gagctccctg gagagaacga tctggctggc gcctgcctga
     1141 aggagcagtt ccacacctgg cgtgttatgc agcgtcatct gcgttgatac gtgtcgctct
     1201 agtccatcca aatcctctgc taaaagccag agtccgagat cctggatgat aaccccatct
     1261 acgccggctt cgtggagaaa cgatacaaac tccgctgcct cttgcagttc gccgttttta
     1321 atcaggatgt ttacggtaac gtaaaccttg acattgtgtt ttttgcagta cgcgactgct
     1381 tcctctattt ctgcgttgga aaaattagcg gcagatgctc ttgcggaaaa caatttcccg
     1441 cctagatata cagcatcagc accgctttgt accgcggcag ttaggctttc aaagctgcca
     1501 gcaggtgcaa gcagctctat tttctggttc ggcatggcca aatcgataac tcctcttttc
     1561 aagctattag acatcattct tattatacca aatatacctt ggcaagttgt ggaaaacact
     1621 gttcggaacc gacatggttc ctaaatgcca agaccgaaaa aactccgcct tctggcgacc
     1681 ccttctagtg catacaccgg gtgctttttg agagcattgg gatctgagac ggggcctgct
     1741 tccaagatgc ccagaagctg atctttgaaa accggtgcct cgagatcgta gggaacaaac
     1801 ctaacgtatg gttttactcc tttgggaacg gtgacgaaca catcccccat gactccacct
     1861 acagcatagc ttttggcgtt tttcacgtag atatctggga tggtttcgtt tttaccgaca
     1921 actttgatac acagggtatt ggcaaacccc cactccagaa gccgtgccga ctcaacccag
     1981 cggttagaac tgttcaacac caccgcgatg agctgcctgt cgtctctagt ggcagaagac
     2041 accaggcatg ccccggctag gttggtggtg ccggtcttta ccccatctgc atcgggcatg
     2101 atccaaagga gtttgttagt gtttttcaag tacttcgcaa aagactgctg ccagggaatc
     2161 tccttttctt tactgctgac aatctcttgg aagagtgggt atttgagcgc atggctggcc
     2221 ataatggcaa gatccctcac agtggtgtag tggttgggat cggacaagcc gtggggattt
     2281 acgaagtgtg tctgccggct gccgagctca gcagctctag tgttcatgta cttggcaaac
     2341 tcctccacag agcctgcgat gtgctctgcc agtgcgatgg cagcatcgtt tcctgaaagg
     2401 agcaaaaggc cccacagaag cttttcgacg gtgtagactt ctccttccct tagacctact
     2461 ctagaaccgt tgatggtcgc agctctacgg ctaactgtca cagactcccc aagagagcac
     2521 cgctccaacg tgagaatggc cgtcatgatc tttgtggtag aagctggtgc tcgctgggaa
     2581 tcggcgtttt tggcgtagag gatggtgccg cttttagggt cgaagagaat cgctgctgaa
     2641 gcgcccacat caggtttgct gcggaaaagg ctgctgatat cggtgacctc gctgagtcct
     2701 gccaccgtca ctaccgcttc atcagtcttc gggggttgca tgatgaggat gaccaaaaga
     2761 aaaatggcta taatcaagac aaaacgcatc tgcctcatat cttttcctcc tgaaagagtc
     2821 cttctttcta agatatgaac ggcaaaatcc gtttatcttt ccttagggaa ataaaccgct
     2881 ggaggtaggc caatgacaaa actgcaacac tcaagctcca gtttctaagc aaaaaaccag
     2941 ggagagcagt cagctctccc tggggttctg aagctgttat tggtttttca ctacggcgta
     3001 gggcagggtt tgtagaacat cttcttcgcg ggtgtcgaca atgacaaact catctcccgg
     3061 ttcgatggcg atctctctcc ggaggagaag tctcttgaag cctgcaatgg aacggataat
     3121 tcccgcttcg ccgtttcggg tgttaatcaa ggtcttgcct tccaagtact ttgttgaact
     3181 ctc
//
LOCUS       DAOSDA010000196         3180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_19524_length_3180_cov_3.741440, whole genome shotgun sequence.
ACCESSION   DAOSDA010000196 DAOSDA010000000
VERSION     DAOSDA010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19524_length_3180_cov_3.741440"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..>1550
                     /locus_tag="PLW82_05310"
     CDS             172..>1550
                     /locus_tag="PLW82_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOJ46725.1"
                     /translation="MIDVPGMFGALVFGDNVMKAMLPKGVYEALRKTAVNGRHLEPGV
                     ADAVAAAMKEWALRNGATHYAHWFQPLTGASAEKQEAFISPCGQGKVIMEFSGKDLIK
                     GEPDASSFPSGGLRDTAAARGYTAWDPSSYAFIKDGTLYIPTAFCSYSGEALDQKTPL
                     LRSMQALDEQALRILRLFDCHTVQRVVSTIGPEQEFFLIAKEVFLRRADLVYCGRALF
                     GARPPKGQELADHYLAPMNRRVAAFFQELEQELWKVGVPVKTKHSEVAPGQYELAVVY
                     ETINTAADHNRLGMELLKSTADKHDLVCLLHEKPFPWVNGSGKHINWSMTTDTGENLL
                     NPGDEPHKNLRFLLFLTAVIAAVDEHQDLLRLSVASAGNDHRLGAHEAPPAIISVYLG
                     DELTEILQSIANGSRCQGMEKQLMETGAMVLPRIAKDAADRNRTSPFAFTGDKFEFRM
                     PGSAMSVAGP"
     assembly_gap    1551..1560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1561..2273
                     /locus_tag="PLW82_05315"
     CDS             <1561..2273
                     /locus_tag="PLW82_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOJ46726.1"
                     /translation="TVLNTIIADSLKQFADALEGSSNLREDLIGVIKEKIQKHFRVVF
                     NGDNYSQEWVVEAGCRGLLNLRTTPEALPYLLETKNIKLFERHQVLTKAEVHARYETL
                     MESYCKTIQVEAATMIELVKKEVMPVALSFQRDLAQIIRDKKWASAQIDCGPEEALLD
                     KLACLTRYLYNLHLILEEKISALRDINDLAHKANYSRDEILGCMQKLKTIADELEAAA
                     PKANWTLPSSGEILYSAR"
     gene            complement(2430..>3180)
                     /locus_tag="PLW82_05320"
     CDS             complement(2430..>3180)
                     /locus_tag="PLW82_05320"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOJ46727.1"
                     /translation="SNYFYEIANLIPNDYQPFVGKAAFAHKGGVHVSALRKNPGTYEH
                     IEPEVVGNRRRVLVSELSGIASLSYKAEEYGIEGERDMFGPILDTVKNLEYKGYSFEG
                     AEASFELLLKRSLGLVPEFFSLKGLRMIIEKNGNGSFVAEATVKIQVGDKVYHTVSEG
                     DGPVNALDRALRLALRDVYPEIENISLTDYKVRVLDQKSATAASVRVLIESSGFGASW
                     GTVGVSPNIIEASWNALFDSIVYGLYKSREV"
BASE COUNT          773 a          870 c          805 g          722 t
ORIGIN      
        1 tatactagta tttttctgcg gacgattgta tacggtgtta tcaaacggca gtgattgaca
       61 gttggaactg gcggtggtat actcaacgac gtgatgcagc aaaggcgctg cgggcttttt
      121 tgggcccgca gtgcctttat ttttcggcta acgaggaaga aggtagaagg catgatcgat
      181 gtacctggta tgttcggtgc tttggtattt ggcgataatg taatgaaggc gatgctccct
      241 aaaggcgtgt acgaagcttt gagaaaaacc gcagtaaacg gtcggcactt ggagcctggc
      301 gtcgcagacg ccgtggctgc ggctatgaag gaatgggcac tccgaaacgg cgctacccac
      361 tatgcccact ggtttcaacc cctgaccgga gcttctgcag aaaagcagga ggccttcatc
      421 tccccatgcg gccaggggaa agtgatcatg gagttttccg gaaaagatct gatcaaaggc
      481 gaacccgacg cctcgagctt cccctctgga ggactcaggg acactgctgc agctagaggt
      541 tacaccgcct gggatccgtc gtcatacgcc ttcattaaag acggtaccct gtacattcca
      601 actgcctttt gctcctacag cggcgaagcg ctggaccaga aaacccctct cctcaggtcg
      661 atgcaggctt tggacgaaca ggctctccgc attctgagac tcttcgattg ccatacggtc
      721 cagagggtgg tctccaccat cggtccggaa caggagttct tcctgattgc aaaggaagtt
      781 tttctcagaa gagccgacct agtctactgc ggacgtgccc tctttggagc aagacctcca
      841 aaagggcagg agcttgcaga tcactactta gcccccatga accggcgggt tgcggcgttt
      901 ttccaagagc tcgagcaaga gctctggaag gtgggcgttc cggtaaaaac caagcacagt
      961 gaggttgcgc ccggacagta cgaactggcg gtggtttacg aaaccatcaa caccgctgcc
     1021 gaccacaacc ggttgggcat ggaacttctt aagtccactg cagacaagca cgatctggtc
     1081 tgcctgctgc acgaaaaacc cttcccttgg gtaaacggga gcgggaagca cattaactgg
     1141 tccatgacca cagacaccgg agaaaacctt ctgaatcctg gagatgagcc tcacaagaac
     1201 ctgaggttcc tcttgtttct aacagcagtg atcgctgctg tagatgagca tcaggatctt
     1261 ctgcggcttt ctgtggcaag cgccggcaac gaccaccgct tgggagctca cgaagcgccg
     1321 cctgccatca tctcggtcta cctgggcgat gagctcaccg aaatcctgca gtccattgca
     1381 aatggcagca ggtgtcaggg tatggaaaag cagttgatgg agaccggtgc catggttctt
     1441 ccccgcattg cgaaagatgc tgcagaccgc aacagaacct caccatttgc cttcaccggt
     1501 gacaagttcg agttccgaat gcccggatct gccatgtcgg tggctggtcc nnnnnnnnnn
     1561 gcaccgtttt aaacaccatt atcgccgatt ctctcaagca gtttgccgat gcccttgaag
     1621 gcagctccaa cttgagagaa gacctaattg gcgtcataaa agaaaagatc cagaagcact
     1681 tcagagtggt ctttaacggg gacaactact cacaagagtg ggttgtggaa gctgggtgca
     1741 gagggttgtt gaacctgcgc actacccctg aagccctgcc ctacctgctt gagaccaaga
     1801 acattaagct gtttgaaagg caccaagtgc tgacaaaagc cgaagttcat gctcgctacg
     1861 agaccctcat ggaaagctac tgcaagacca tccaagtgga agctgcgacc atgatcgaac
     1921 ttgtgaaaaa agaggtcatg cctgtagcgc tgagcttcca acgggatctt gcccagatta
     1981 tcagagacaa aaaatgggca agtgcccaga tcgactgcgg acctgaagaa gctcttctgg
     2041 ataaactagc ctgccttacc cgctaccttt acaatctaca cctcatcctt gaagaaaaga
     2101 tatccgcact tagggatatc aatgacctgg cccacaaggc caactactcc agggacgaaa
     2161 tcttgggctg tatgcagaaa ctgaaaacta tcgccgacga actcgaagct gcagctccga
     2221 aggccaactg gacgcttcct tcaagcggcg agatcctcta tagcgcccgg tagttctgcc
     2281 tgacgccccg ccctccacta aaagagccgc aaacgaagga gccgcacagg gcttgcccta
     2341 cttaagtagg tgcaagcccg tactttcttt taaaacagaa cagctgccca aacggcagct
     2401 gcttttgcat atgaattagt gaggttgttt tacacttccc tggacttata caacccgtac
     2461 acgatactgt caaaaagagc gttccagctc gcttcgatga tgttcggcga aacgccgaca
     2521 gttccccaag aagcaccaaa cccgctgctt tcaatgagaa ctcttactga agctgctgtg
     2581 gcactcttct ggtcgagcac cctcaccttg tagtctgtaa ggctgatgtt ttcgatctcc
     2641 gggtacacat cccttagcgc cagcctcagc gccctatcca gagcgtttac cggaccgtcg
     2701 ccttcgctta cagtgtggta gaccttgtct cctacctgga tctttacggt agcttccgct
     2761 acaaagcttc cgttgccgtt tttctcgatg atcatcctga ggcctttcag ggagaaaaac
     2821 tcaggtacaa gacctagaga tcttttcaag agcagttcga aggacgcttc tgcaccttca
     2881 aaagagtacc ccttgtactc caggttcttc accgtatcca ggataggccc aaacatgtct
     2941 ctttcgcctt cgatgccgta ctcctctgct ttgtatgaga ggctggcaat gccgcttagt
     3001 tcggacacca ggaccctacg cctgttgccc acaacctcag gttcgatgtg ctcataggtc
     3061 cccgggttct tccttaaggc cgagacgtgc acgccccctt tatgggcaaa tgcagctttc
     3121 cctacaaacg gctggtaatc gttggggatg aggtttgcaa tctcatagaa gtaattgctg
//
LOCUS       DAOSDA010000197         3180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_19528_length_3180_cov_3.104000, whole genome shotgun sequence.
ACCESSION   DAOSDA010000197 DAOSDA010000000
VERSION     DAOSDA010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19528_length_3180_cov_3.104000"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLW82_05325"
     CDS             <1..462
                     /locus_tag="PLW82_05325"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOJ46728.1"
                     /translation="ILYSVGRLVSVKTLELIFDSKYAKIVHLRKSDVRRAGHWFVKHG
                     NLAVFLGRFVPVIRSLVSIPAGIAKMKLGPFLALTFLGTSIWNLVLVYLGKMAGDAWE
                     SVNRYMDIYFLISIVILAALGAYIAARFIKSRFLVRQNLPEAEGEEGQGEE"
     gene            615..1475
                     /locus_tag="PLW82_05330"
     CDS             615..1475
                     /locus_tag="PLW82_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOJ46729.1"
                     /translation="MIKITADSTCDLSPEILKSLDIDIVPLSIVVDEKSYKDGVDIQP
                     SDIFDFVENEKKACKTTAVNSFEYGNVFKKHLKEYDAVIHINISSEFSSCYQNARIAA
                     AELENVYVVDSRNLSTGSGHIVMDAALMAKEGVEPAEIVRRLEETIPKVDASFVINTL
                     DYLYRGGRCSGLEVFGANLLKIKPSIEVVGGKMKVGKKYRGNLKLCLENYIKDKFKDA
                     SDIDPSRLFITHTPVPEGTVEMIKELVGQYVQFEEIIETTAGCTISAHCGPGTLGVLF
                     KRLSTKQNGV"
     gene            1701..2369
                     /locus_tag="PLW82_05335"
     CDS             1701..2369
                     /locus_tag="PLW82_05335"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HOJ46730.1"
                     /translation="MALKVISQTPPQTSQERTTESGDIKQLSKQLMEMQGVYDAAIKK
                     VCEKLEAINCKFQDQFDHSPIRFVETRLKTAESIYKKLKKRGCPVSPESARKNLSDIA
                     GIRVVCYFIGDAYAIADKLLQESDIILIEKKDYIEKPKANGYRSLHLVVQLPVVQSGK
                     AELVPVEIQIRTMMMDLWASLDYQLKYKKEQAVDPQIIQCLKECAEKIAELDNRVQDI
                     CRKI"
     gene            2536..>3180
                     /locus_tag="PLW82_05340"
     CDS             2536..>3180
                     /locus_tag="PLW82_05340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOJ46731.1"
                     /translation="MKQYFDYKDQRLEFELVHRKRRSIEIQIDESGIRVIAPVGVSPH
                     ILQQVLQDKGDWILSKLAILKNRKTISHTFTKGDRILYLGKEYELEVRPVLGRPYVEL
                     GESTITVSTSRRDPEDVKRTLETWYKKQAKELARSRAEHYRKILDVQPVEVKISSAKK
                     RWGSCSPKGNILINWRLVMAPPWVFDYVVAHEMCHLIEMNHSKDFWQLVERAVPH"
BASE COUNT          905 a          713 c          847 g          715 t
ORIGIN      
        1 atcctctaca gtgttggcag gctggtaagc gtaaagaccc ttgaacttat tttcgactct
       61 aagtacgcca agatcgtcca tctgcgaaag agcgatgtgc gcagggcagg gcactggttc
      121 gtaaaacacg ggaacctggc ggtctttctc ggtagatttg tgccggtgat acggagcctg
      181 gtgtccattc ctgcaggcat tgccaagatg aagctaggtc cgtttttggc gctcaccttc
      241 cttggtacca gtatctggaa tctggttctg gtctacctgg gaaagatggc gggagatgct
      301 tgggagtcgg tgaacaggta catggatatc tacttcctca tttccattgt gatcctcgca
      361 gctctcggtg catacatcgc cgcaaggttc attaagagca ggttcttagt ccggcaaaac
      421 ctgcccgagg ccgaaggcga agaaggccag ggggaagagt agagggtttc ccggtgcagt
      481 gggcgactgc gagtttcgga agattagttg ctaaaagtag caggaaaaat gcaccgtgat
      541 gagtaattcc tcttaggtcg accttacaag cattactaaa tgcagtttat acaccgaact
      601 agaggaggga ttttgtgata aagattacag ccgatagtac ctgcgatcta tctcctgaaa
      661 ttctaaagag cttggatatc gacattgtgc cgctgtccat cgtagttgac gaaaagtcgt
      721 acaaagacgg tgtggatatc cagcccagtg atatattcga ttttgttgaa aacgaaaaga
      781 aagcttgcaa aactacagct gtaaacagct ttgaatacgg taacgttttt aagaaacacc
      841 tcaaagaata cgatgcagtg atccacatta acatcagctc ggagttctca tcctgctatc
      901 agaacgcccg cattgcagct gctgaactcg aaaacgtcta cgttgtcgat tcgaggaatc
      961 tttccaccgg cagtgggcac atcgttatgg atgcagcact gatggcaaag gaaggcgtag
     1021 aaccggcaga gatcgtacgc agactggaag agaccattcc gaaagtggat gccagctttg
     1081 tgatcaacac tctcgactac ctgtacagag gcggccgctg ctcgggacta gaagtgttcg
     1141 gggcaaatct gttgaagatc aaaccttcaa tcgaggttgt tggcggcaag atgaaagtcg
     1201 gcaagaagta cagaggcaac ttgaaactct gccttgagaa ctacatcaag gacaagttca
     1261 aagacgcatc agacatcgat ccttcgagac tgttcatcac ccacacgccg gttcccgaag
     1321 gtacagtaga gatgattaaa gaactggtcg gtcagtacgt ccagtttgag gagatcattg
     1381 aaaccactgc aggctgcacg attagcgcgc actgcggacc cggcactttg ggcgttctct
     1441 ttaaacgtct cagcactaag caaaacggcg tgtaaaaggg tagaatccct ttgggaggcg
     1501 ctctcaaata gggtaaacgc aggagacgat ttttcgtctc cttttttctt cttgcagaat
     1561 ttgctctgca gattgcactt gagactccgg gaaatctagc tgagaaaaaa agagggatag
     1621 cggcagattt acttaagtta tacagtagaa gactgccgct ttgaggcaga gctacatgtt
     1681 tactccaaag gaggatgcct atggcactga aggtgataag tcagactcct ccacaaacat
     1741 cacaagaaag aaccaccgag agtggagaca taaagcagct atctaagcag ctaatggaga
     1801 tgcagggggt ctacgatgcg gctatcaaga aggtgtgcga gaagctggag gcgattaact
     1861 gcaagttcca ggaccagttc gaccacagcc ccatcagatt cgttgaaact cgcttgaaga
     1921 ctgccgaaag catctacaag aagctgaaga aaaggggatg cccggtcagt cccgagtcag
     1981 ctaggaagaa tctcagcgat attgcaggaa taagggtggt gtgttacttc atcggcgatg
     2041 cctacgcaat tgcagataag ctcttgcagg aaagtgacat catccttatc gaaaagaagg
     2101 actacattga aaagccaaag gccaacggct accgcagcct ccaccttgta gttcagcttc
     2161 ctgtggttca gtccgggaag gcagagcttg tgcctgtgga aatccagatt cgcaccatga
     2221 tgatggactt atgggccagc ctcgactacc agctgaaata caaaaaagaa caggcagtgg
     2281 atccccaaat catccagtgc ctgaaggagt gcgccgaaaa aatcgcagag ttggacaaca
     2341 gggtccagga catctgccga aagatctaaa gaaaaaccgt ccctgcttgc gcagggattt
     2401 ttagttgaaa acaatgccta caggtcgccg tgaaaacctt tctaattgtg cgccaaaact
     2461 gcaaaactaa aattataggt ttttaatgac tacctaagtt gtcgagaacg ggctttacgg
     2521 attagaggga ttttagtgaa acagtacttc gactacaaag accaacgact ggagtttgag
     2581 ttggtgcata gaaaacgcag gtccattgag attcaaatag atgaatccgg gatcagggtc
     2641 atcgctcctg taggtgtcag cccgcatata ctacagcagg ttttacagga caagggggac
     2701 tggattttaa gtaagctggc gatactgaaa aaccggaaga ccatctccca caccttcacc
     2761 aaaggtgaca ggatcctgta tcttggaaag gagtacgagc tcgaggtgcg ccctgttttg
     2821 ggaaggccgt atgtggagct cggtgagagt accattaccg tttccacttc cagacgcgac
     2881 ccggaagatg tgaagcggac tttggagacg tggtacaaaa agcaggcgaa agagctagct
     2941 aggtcaaggg cagagcacta caggaagatc ctagacgtgc agccggtaga ggtgaagatc
     3001 tcttctgcca agaagcgctg gggaagctgc agtccgaagg ggaacatcct catcaactgg
     3061 cggttagtca tggctccacc ttgggtattt gactacgtag tggcccacga gatgtgccac
     3121 ctaattgaaa tgaaccactc aaaagacttc tggcagctgg tggaaagagc ggtgccccac
//
LOCUS       DAOSDA010000198         3177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_19563_length_3177_cov_2.662716, whole genome shotgun sequence.
ACCESSION   DAOSDA010000198 DAOSDA010000000
VERSION     DAOSDA010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19563_length_3177_cov_2.662716"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..370)
                     /gene="rpmA"
                     /locus_tag="PLW82_05345"
     CDS             complement(86..370)
                     /gene="rpmA"
                     /locus_tag="PLW82_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOJ46732.1"
                     /translation="MLRINLQFFASKKGVGSSKNGRDSEAKRLGVKRFGGQLVTAGSI
                     LVRQRGTKFYPGENVGLGRDHTIYALIDGYVTFEPKGKNGKKVSVYQVAE"
     gene            complement(373..708)
                     /locus_tag="PLW82_05350"
     CDS             complement(373..708)
                     /locus_tag="PLW82_05350"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOJ46733.1"
                     /translation="MTKITVIRDERIKKVEIRGHAGFAEKGEDIVCAGISSLSFAAGM
                     ALERYGIPAEIHQDEENASFAIIPNWSDMTEKQALRTETILQSLVWALEDLAESYPRY
                     VELDTVRRC"
     gene            complement(708..1019)
                     /gene="rplU"
                     /locus_tag="PLW82_05355"
     CDS             complement(708..1019)
                     /gene="rplU"
                     /locus_tag="PLW82_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOJ46734.1"
                     /translation="MKYAVIQTGGKQYTVSEGDVIRVEKLDAEPGSVVDFDKVLLIGG
                     DETKVGTPFVEGAKVSAEVVKSGKGKKIIVFKYKAKKNYRKKQGHRQPYTELKITAVE
                     G"
     misc_feature    complement(1031..1107)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(1173..2612)
                     /locus_tag="PLW82_05360"
     CDS             complement(1173..2612)
                     /locus_tag="PLW82_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOJ46735.1"
                     /translation="MKRKILINVGYKEKRIAVLEDGKLVEIYFDRNLDGEAAFGSIYK
                     GKVSNVLPGMEAAFVNIGQEKNAFIHASDIDHSKNGPKKIQKLVHPGQEILVQIKKEA
                     VGTKGAKVTAALSIPGSYLVLLPTDRGIGISRKIEDEEERNRLKEIADEIKPEGMGLI
                     IRTAARGADYARLKLDLDRLLEIWNEIERKAKSVKAPSLLFSDEELTLRVVRNLLGRE
                     IEEIVVDSDQGFLKLKELVGEKDVQITLYSDKEPMFVHYDIESQIQRALRNKIWLDCG
                     GFLIIDQTEALSIIDVNTGKFTGKDSLKNTVLTTNLEAAEEIARQLRLRNIGGIIIVD
                     FIDMDNPKDREKVRSRLEECLKKDHTRTSILGFTSLGLLEMTRQKTSHDLGATLQKRC
                     PTCGGTGKVPDEETISLDLLRELKFFIRDSSSLEFTVKANPAVVAKACEIADDLEEYQ
                     KRLGVKILFEPDWEKKLSEYQIFEGIGGK"
     gene            complement(2627..>3177)
                     /locus_tag="PLW82_05365"
     CDS             complement(2627..>3177)
                     /locus_tag="PLW82_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOJ46736.1"
                     /translation="EAKLPLSFSEGFSPRPKLVFAHALGLGITSSGEYLDVALDEDVD
                     PGEVFFRLKEALPSPFPLYDCAALPNGVTPAMAAITHASYVFTPEDESRILQINSMDT
                     LISTRNRKGKVVTEDIRPHISLVEKTGSGVRVIVRAGSQKNVRPDALLAALGIEEPVP
                     VHREDLYIETGGRLITPLMRTE"
BASE COUNT          680 a          893 c          721 g          883 t
ORIGIN      
        1 gagatagcca agttgccttt agccgacttt tcaaaaaagc cctccgggat ctcccgaagg
       61 gctaatctaa caagtccagc tgtccttact cggctacttg atatacgcta acctttttac
      121 cattcttgcc ctttggttca aaagttacat agccgtcaat gagtgcgtag attgtgtgat
      181 ctctgccaag tcctacgttc tcgcctggat agaacttggt gcctctttga cgaacaagga
      241 tgctcccagc tgttactagt tgtccaccga acctcttgac gccaagtcgt ttagcttctg
      301 aatcacggcc gttcttggaa ctgccgacac ccttcttgga tgcgaaaaac tgcagattaa
      361 tccggagcat tcctaacacc tcctcaccgt gtctagctcc acatatctag gatagctctc
      421 tgctaaatcc tctaaggccc atactaggga ctggagaatc gtctctgtcc ttaaggcctg
      481 tttttcggtc atgtccgacc agttggggat tatcgcaaaa gacgcatttt cttcatcctg
      541 atgaatctct gcaggtatcc cgtagcgctc tagcgccata cctgcagcaa aagacaggct
      601 tgatataccg gcacacacaa tgtcttcgcc tttttcggca aagccggcgt ggcctcttat
      661 ctcaaccttt ttgattcttt catcgcggat aactgtaatc ttagtcatta gccttcaact
      721 gcggtgatct tcaattctgt gtaaggttga cggtgacctt gcttcttgcg gtagttcttc
      781 ttggctttgt acttgaagac gatgatcttc ttgcccttgc cgcttttcac tacctcagcg
      841 ctaacctttg cgccttcgac gaaaggagtt cccacctttg tctcatcacc gccgataaga
      901 agcaccttat cgaaatcgac aacgctacct ggctctgcat cgagcttctc cactctgatg
      961 acgtcgcctt cacttactgt atattgcttc ccaccagttt gtatgactgc gtacttcaac
     1021 ctgtccacct ccattctctt agactcgcca gaggtaggcg cgtaccgagt acgtcttaaa
     1081 gcccaatctg agcggttttg agcaacaggt tgctactcac tcatgtaagt ataccatcta
     1141 actatctggt tgtaaataac ccctaaaagc atttatttac cgccgatacc ttcgaaaatc
     1201 tggtattcgg agagcttctt ctcccagtcg ggctcgaaca ggatcttgac ccccaaccgc
     1261 ttctgatact cttccaggtc gtcggcgatc tcgcaggctt ttgctaccac agcgggatta
     1321 gccttcaccg taaactccaa gctgctgcta tccctgatga agaacttgag ctccctgaga
     1381 agatccaagg agatggtctc ctcgtcggga accttcccag ttccgccgca tgtaggacac
     1441 cgcttctgca gcgtggcacc aaggtcgtgg ctggtcttct gccttgtcat ctccaagaga
     1501 cccaaagagg taaaccccag gatgctagtt ctggtgtgat cctttttcag gcattcttca
     1561 agcctgctcc tgaccttttc cctatcttta ggattgtcca tgtcgataaa atcaacaatg
     1621 atgatgccgc cgatgtttcg gagccgaagc tgccttgcta tctcctctgc agcttccaaa
     1681 ttggtagtaa ggacggtgtt ttttaaagag tccttaccgg taaacttacc ggtgttgaca
     1741 tcgatgatgg acagggcttc cgtttggtcg atgataagaa aacccccaca gtccagccag
     1801 atcttgttcc gcagtgcccg ctggatctga ctttcgatgt cgtagtggac aaacatcggc
     1861 tccttatccg agtaaagggt gatctggacg tccttttcac caaccagttc tttcagcttc
     1921 agaaatccct gatccgaatc gacgacaatc tcttcgatct cccgccccaa aagatttctc
     1981 accaccctga gggtcagctc ctcatcggaa aagagcagcg atggggcttt tacgctcttg
     2041 gcctttcttt cgatctcgtt ccaaatctcc agaagcctgt ccagatccaa cttcagccgg
     2101 gcatagtcag cgcctctggc tgcggtgcgg atgatcaggc ccatcccttc aggcttaatc
     2161 tcgtcggcga tctccttcag ccggtttctt tcttcttcat cttcgatctt tctggagatg
     2221 ccgatacccc tgtctgtagg aagaagcacc aggtaactcc cggggatgct cagggcagca
     2281 gtgacttttg cacctttagt gcccaccgcc tcttttttga tctgcaccaa aatctcctgg
     2341 ccgggatgga ccagtttttg aatcttcttg ggaccatttt tgctgtggtc gatatcggac
     2401 gcatggataa aggcgttttt ttcctggccg atgttgacaa aagcagcttc catgcccgga
     2461 agcacgttac tgactttgcc tttatagatg ctgccgaagg cagcttcgcc gtccagattt
     2521 ctgtcgaaat agatctcaac gagctttccg tcttcaagga cggctatgcg cttttctttg
     2581 tagccaacat taattaaaat tttccgcttc acgtggagtc atctccctac tctgtgcgca
     2641 taagtggcgt aatgagccga cctcccgttt cgatatagag atcttctcga tgaactggca
     2701 caggctcctc tatgccaaga gctgcaagga gcgcgtcggg gcggacgttc ttttggcttc
     2761 ccgcccgaac gatcacccgc accccagacc cggtcttttc caccaggctg atgtggggcc
     2821 tgatatcttc ggtcaccact ttgccctttc tgtttctggt ggagatcaag gtgtccatgc
     2881 tgtttatctg caagatgcgg ctctcgtcct ccggggtaaa aacgtagctg gcatgtgtga
     2941 ttgccgccat agccggagtc acgccgtttg gtagagccgc gcagtcgtag agtggaaagg
     3001 gactaggcag tgcttccttc aggcgaaaaa agacttctcc cggatcaacg tcttcatcca
     3061 gcgccacgtc taggtactcg ccggagctgg tgatgccaag acctagcgcg tgggcaaaca
     3121 ccagcttagg tctgggcgaa aagccttcag aaaagctgag aggtagcttt gcctccg
//
LOCUS       DAOSDA010000199         3161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_19693_length_3161_cov_3.533162, whole genome shotgun sequence.
ACCESSION   DAOSDA010000199 DAOSDA010000000
VERSION     DAOSDA010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19693_length_3161_cov_3.533162"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PLW82_05370"
     CDS             complement(<1..1126)
                     /locus_tag="PLW82_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46737.1"
                     /translation="MNKKIFALIVVLVLLSNLALAKTLTVWITGHSNEELNIMKEMID
                     EQFSPKFGVEVAVTGLSWADNESKYLLAAASGDVPDVGSAGGLFLPELGLRGALVNLT
                     DFPDFEEVRKRAPEGFYRSLTYDSLVFGVPYFSSLTVAYYRDDILQNLGLGPIETWDD
                     LRRALPKMQANNTNFSLSWGLTNTVYSDINLFMWQNGGDDYTPDLQRSGLDMPGSIKG
                     FTEFVELYTVYKIPMETPLFQGFINGDLAIMLSGQAMYANLMNGAPQLQGKWSLREVI
                     GTMQDGEINRAATGTGWSMGIFKESKNKELAWEFIKWFTSEEVQLEMTKRITERITGS
                     MFLPANLASLQHIPVADADRQTFLRQLEVSKASVFGLVSPN"
     gene            1309..1905
                     /gene="yqeK"
                     /locus_tag="PLW82_05375"
     CDS             1309..1905
                     /gene="yqeK"
                     /locus_tag="PLW82_05375"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOJ46738.1"
                     /translation="MKDWYEVDNIQVLEFVRSRVTQARFEHSKRVADMAVRLAKAHGF
                     SEENAYLAGILHDICREYPGEKLLELAEQYQITVDEYERVWPIGLHGKVAAEELRTWG
                     LDEEVLEAIRYHVSGYPGMGSLARILYLADKIEEGRSYPGVEELRLLALKDLDGALSD
                     AIDHSFLYLIRRKLPIHPNTLACRNELLLKNVLPKGIK"
     gene            1935..3161
                     /locus_tag="PLW82_05380"
     CDS             1935..3161
                     /locus_tag="PLW82_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOJ46739.1"
                     /translation="MKNVKKTSLRIIGYVSLAFLLIAAALALTIYLTLVSLNDDSSWV
                     ASQRRDVLEDVEAPGVYQYHERTNFLVLGADKAGGVERTDVMIVVSVDEKRANAALIS
                     IPRDSKVTVKGRTEKINAVFPNHGIITTMQVVQDLLNIPIHYYIKMDFEGFEKIIDTL
                     GGVIIDVEMPMVYDDYSQNLHIRLMPGRQRLNGKESLHYVRYRGDGFGDVTATNSGYV
                     GRVARQQKFIEALVNEASRPSNLGKLPALTTQLKDAVRTNLTPAEMLKWATLAQSSRG
                     FQIKSFVAPGRPETIGDASYWVIDEKQLAEIVNEYFTVGRYGFSVGIMNGSGNLDAAR
                     KFAEELREKGFKVSGVSEVKDYQYQYTVIQAREEFADAIEWLKVNLGLGDKEWIKADS
                     PLGDVTILLGRDMINR"
BASE COUNT          865 a          681 c          823 g          792 t
ORIGIN      
        1 tatttggaga tactaaacca aatacagatg ccttagacac ctctaactga cgcaggaaag
       61 tttgcctgtc ggcatcggcc actgggatgt gctggagaga agccaggttg gcaggaagga
      121 acatcgagcc tgtgatgcgt tcggtaatcc ttttggtcat ttccagctgg acttcttcag
      181 atgtgaacca cttgataaac tcccatgcta actctttgtt tttcgactct ttgaagatac
      241 ccatagacca cccggtgcct gtggctgcgc ggttgatttc accatcttgc attgtaccga
      301 tgacctctct caaactccat ttaccttgga gttgaggagc accgttcatc aggttagcgt
      361 acatcgcctg gccggagagc ataatggcca aatcgccgtt gataaagccc tggaacagcg
      421 gagtttccat agggatcttg taaactgtgt acagttccac aaactctgtg aaccccttaa
      481 tgcttccggg catatcgaga ccggacctct ggagatctgg ggtgtagtca tcgccgccgt
      541 tttgccacat aaagaggttg atgtcagagt agactgtgtt tgtcaggccc catgagagcg
      601 aaaagttggt gttgttagcc tgcatcttgg gcagtgcgcg cctcagatca tcccaagtct
      661 cgataggacc gagtccgaga ttctgcaaga tatcatctct gtagtaggca acggtaagcg
      721 aagagaaata cggtacaccg aaaaccaaac tatcgtaggt tagcgagcgg tagaaacctt
      781 cgggagccct cttccggacc tcttcgaagt cgggaaaatc cgtgagattt accaaagcac
      841 ctctaagtcc gagctccgga aggaacagtc cgccggcact acccacatcg ggtacgtctc
      901 ctgatgcagc agcgagaagg tatttggact cgttatctgc ccagctgagt ccagttacag
      961 caacttcaac gccgaatttc ggcgagaatt gctcgtcgat catctctttc atgatgttca
     1021 gttcttcgtt actgtgcccg gtgatccaca ccgtaagtgt cttggctaaa gccaggttac
     1081 tgagaagtac aagaacaacg attaatgcaa aaatcttttt gttcaaaacc cacaccccct
     1141 gtacatattg agtgattgta cccctcaaaa gagttcgtga tttgggcaag atcacctctt
     1201 ttgtctggat ctgattttct ccgaaagccg tttgcggaga tgtccaaaaa tgcttggcag
     1261 ttgcaggcat ctctttttca atggagaaag aattatgtag aaagcacatt gaaggattgg
     1321 tatgaagtgg ataatataca agttttggag tttgtgcgga gcagagtaac tcaagcgaga
     1381 ttcgaacact caaagagagt ggcggatatg gctgtaaggc tggcaaaagc tcacggtttc
     1441 agcgaagaaa atgcatatct tgcaggaata ctgcacgata tctgcaggga gtatcctgga
     1501 gagaaattgt tggaacttgc ggaacagtac cagattacag tcgatgaata tgaaagagta
     1561 tggccgattg gactgcacgg aaaggttgct gcagaagaac tccggacgtg gggtttggat
     1621 gaggaggttc tggaagcaat ccgatatcac gtatctggtt atccgggaat gggaagcctt
     1681 gcacgcatcc tctatttggc agacaagata gaagaaggca ggagctatcc cggagtggaa
     1741 gagctgaggc ttcttgcact taaagatttg gacggtgcac tctcggatgc gatcgaccat
     1801 tcgtttttgt acttaattag gagaaaactg cccattcacc ctaacacctt agcttgcagg
     1861 aacgagcttt tactaaagaa cgttctcccg aaaggtatca aatagagtta ggtttggagg
     1921 ttgctgtagc ttttatgaaa aacgttaaaa aaacttcgtt acgaatcata ggatatgttt
     1981 ctttggcttt cctgctgatt gcggcagctc tagccttaac catatatttg acgcttgttt
     2041 cgctcaacga tgattccagt tgggtagcga gtcagcggag agatgttctg gaagacgttg
     2101 aagctccggg cgtttatcag tatcatgaaa ggaccaactt cctggttctc ggagccgata
     2161 aagccggagg cgttgaaaga acagacgtga tgatcgttgt cagtgtagat gagaaaagag
     2221 ctaatgcagc tctgatctct atccccagag actcaaaggt aactgtgaag ggtagaactg
     2281 agaagatcaa cgcagtcttt cccaatcacg gaatcattac caccatgcag gtggtgcagg
     2341 atctcttaaa cattcctatc cattactata tcaagatgga ttttgagggc tttgagaaga
     2401 tcatcgatac cctcggcgga gtcataatcg acgttgaaat gccgatggta tacgacgact
     2461 attcgcagaa cctccatatt cgcttgatgc cgggaaggca acgcctaaac ggcaaggaat
     2521 ctctccatta cgtgaggtac cgcggcgatg gcttcggaga cgtcacagca actaattccg
     2581 gatatgtagg tagggttgca aggcagcaga agtttattga ggcgctggta aatgaagcgt
     2641 caaggccgtc aaacctcggg aaactaccgg ctctcacaac ccagctgaag gatgccgtta
     2701 ggaccaattt gacacctgcg gagatgctga agtgggctac actagctcaa agcagtcggg
     2761 gatttcagat taagagtttt gttgctccag gtagacctga aacgattgga gacgccagct
     2821 actgggtaat agatgaaaaa cagcttgcag agatcgtaaa cgagtacttc acagttggta
     2881 gatacggttt cagcgtaggt ataatgaacg gcagcggcaa cctcgatgct gccagaaagt
     2941 ttgctgagga actgagggag aagggcttca aagtttccgg tgtttctgaa gtgaaagatt
     3001 atcagtacca gtacactgtg atccaagcac gggaagagtt tgccgatgct atagagtggc
     3061 tgaaagtaaa cctcggtcta ggagacaaag agtggattaa ggcagacagt ccccttggag
     3121 atgtaactat tttgctcggt cgagatatga taaataggta a
//
LOCUS       DAOSDA010000200         3145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_19818_length_3145_cov_3.899353, whole genome shotgun sequence.
ACCESSION   DAOSDA010000200 DAOSDA010000000
VERSION     DAOSDA010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19818_length_3145_cov_3.899353"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="PLW82_05385"
     CDS             complement(<1..1402)
                     /locus_tag="PLW82_05385"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46740.1"
                     /translation="MTFTDLRAADWVVRIEAFGENGAVNAGTDATISVKPGRTVDLSV
                     EIGSGVVSIDPTGGTPPGKPTNLSVDATPKLIKLRSNPALGNASYAVARSTTIDGKRT
                     VLTSSLLTTNEYLEDPTDWKTHQYYYWVAAYDHDGQFSGWAGPILIKKKYSLDITIDR
                     GGSVKRTPDDYGSGYEYGTLVEVEAVPDEGFRFVGWQGDASGKTRLTLGMSSNMKITA
                     VFEPIIPLELKVPEAYPSIQAAIDAAKEGDTIIVSPGTYFENLSFKGKSRITLTSVDP
                     LDPAVVQSTIINGNNKGACITVEYYSTDITISGFTMTGGFRTGKLNSFDRYGGAVCVL
                     QSLRIAITNSIITGNTAQVGGGIYASYSDVTIKDSIISGNTALLSSGGIQTDTSSLTI
                     IGSTLSNNTAMQNAGGIHVAGGTLTITSSEISGNEARDSGGISTLETEDIIITDTVVT
                     GNKSSEYDAGGIRAVYA"
     gene            1875..3074
                     /locus_tag="PLW82_05390"
     CDS             1875..3074
                     /locus_tag="PLW82_05390"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HOJ46741.1"
                     /translation="MRSCQQSRSAGRNLRSGATFLIGVFLAVLLVLLLPAVVSAQDFV
                     TAQEVDVITSKAKVLSISTIVKEETNEFEIDDVYSREIELVEVKVTSGKYRGQTLVIR
                     REASDESLGKEMEVGDKIVIAIVEEAGYFQSAHITDFQRDWLIYLLLGVFVLVLLEVG
                     QVSGVKMLISLIISLLLIAKVLLPSALRGYDPIFLSFAIALFTILITMLLKNDSPEKT
                     WPVVIGSSAGVLLAAVLAFVATKLIHLTGLSQEDIGVLWVLTEGKLNVSSLLVAGVII
                     GSIGAVLHAAVSITHAVEVALALDPFLSVKDLYREGVRVGRDIASTMSSALIFGLIGL
                     ALPLLVHFQASGEHFIEIINMDVVAGLLVKVLAACVGLMAAIPATAISAAYFKKKVAK
                     ADLIDLT"
BASE COUNT          789 a          739 c          765 g          852 t
ORIGIN      
        1 tcgcatagac agccctgatt ccaccagcgt catattcaga agacttgttg ccagtcacca
       61 cagtatcggt gatgatgatg tcttcagttt ccaaagtaga gatgccaccg gagtcacgag
      121 cttcattgcc gctgatttcg ctgctggtta tggtgagggt tccgcctgcc acatgtatgc
      181 cgccggcatt ctgcatagcg gtgttattgc ttaaggtact gccaatgatg gttaagctgc
      241 tagtgtcagt ttgaattcca ccagaggaaa gcaaggctgt gttgccgctg ataatgctgt
      301 cttttatggt aacgtcagag tatgaggcat aaattccacc accaacttga gccgtattcc
      361 cggttattat gctgttggtt atggcaatgc gtaaggattg cagcacacaa acagctccgc
      421 cgtacctgtc aaagctatta agctttcctg ttctaaaacc accggtcatg gtaaatccgg
      481 agatggtaat gtctgtagag taatattcaa ctgtaatgca agcacctttg ttgttgccat
      541 tgataatcgt tgactgtact acagcaggat ccagtggatc gacactggtc agggttatcc
      601 ggcttttacc tttgaagctt aagttttcaa agtaggtccc cggagagaca atgatcgtat
      661 cgccttcttt cgctgcgtca atagcagctt gtattgacgg atatgcttcg ggcactttca
      721 gctccagagg gatgatcggt tcgaagaccg ctgtgatctt catgttagag gacatgccta
      781 acgtgagacg ggtcttgcca gatgcatcgc cttgccaacc aacgaatcta aaaccttcat
      841 caggtaccgc ttcgacttca accaaggtgc catattcgta acccgatccg taatcatctg
      901 gagttctttt tacggaccca ccgcggtcta tggtgatatc tagagagtat tttttcttta
      961 tcagaatagg tccggcccag ccgctaaact ggccgtcgtg gtcgtatgcc gctacccaat
     1021 agtagtactg atgtgttttc caatcggttg gatcttccaa atactcgttg gttgtaagga
     1081 gcgaagaagt tagaactgtt cgttttccgt caatggtcgt gctccgtgct acggcatagc
     1141 tagcattccc caaagccgga ttcgagcgca gttttattag ctttggtgta gcatccacag
     1201 acaggtttgt aggttttcca ggaggtgtgc cacctgtagg atcaatggac acgactcctg
     1261 agccaatttc aacactcaaa tcgacggttc gtcctggctt tacactgatt gtggcgtctg
     1321 ttcctgcgtt cacagctccg ttttcaccga acgcttcgat tctaaccacc caatctgctg
     1381 cccgcagatc tgtaaaagtc acggaattct gcgcttcaga cctagttaac tccttcagcg
     1441 tctgaggttt ccggaaagac agctgcgatc tacaggtttc ccggctgtgc cgcaggaagc
     1501 acgcttcgca agcagccgcc tagtaataca gctaagacga cgaggagaca agctcggtat
     1561 cttttggcca tctaaaactc ctccctgcga aagaaattgt tcacaaccgc aagaattgac
     1621 cattattttt tagcatattt ctgtgtattc cccttacgtg agaagaaacc ttcctgggat
     1681 tgacaaagtt aatttagttt ttgggaaacc aagctcggca agggtagatg cagatagtag
     1741 gttcccgagt ccccataggt ttaccagagg ttgaggggta ttacagaaaa cattcggttc
     1801 gacctatttt gcttgtacgc caaaaatggt aagattatca ttgacgtaca acaccttggg
     1861 atggaggaag aaaaatgagg agttgccaac aaagtagatc tgccggacgc aacctgcggt
     1921 caggagcgac gttcctaatc ggggttttct tggcggttct tttggttctg ttgctacctg
     1981 cggtagtttc cgcacaagat ttcgtcacag cacaagaagt ggatgttatt acctccaaag
     2041 ccaaggtgct aagcattagc accatagtga aggaagaaac gaacgagttt gaaatagacg
     2101 acgtatacag ccgcgagata gaactggtgg aggttaaagt cacttcaggg aagtatagag
     2161 gtcaaacgct tgttattcgg agagaagcat cagatgagtc tttaggtaaa gagatggaag
     2221 taggagacaa gatcgtcatc gctatcgttg aagaagcagg ctacttccaa agcgcgcata
     2281 tcactgactt ccagagagac tggctgatct accttctcct gggagtgttc gtccttgtcc
     2341 ttcttgaagt agggcaggta agcggagtta aaatgctaat tagcttgatc atttccttac
     2401 tcttaattgc caaagttctg cttccctcgg cgctacgggg ttatgacccc atcttccttt
     2461 catttgccat tgcgctcttt acaatcctca tcaccatgct tctgaaaaac gactctccgg
     2521 aaaagacctg gccggtcgtc atcggaagca gtgccggggt cctgcttgct gcagtgctgg
     2581 cttttgtggc aacaaagctg atccacctca caggactctc tcaggaagac atcggcgttt
     2641 tgtgggtgct tacagaaggg aagctaaacg ttagctcact ccttgtggca ggagttatca
     2701 tcggcagcat aggagctgtg ctccatgcag cggtatccat aacgcatgcc gttgaggtgg
     2761 cactagcact tgatccattc ttgtccgtca aagatctata cagagaagga gtcagagttg
     2821 gaagagacat cgcatctacc atgtcaagtg ccctgatctt tggtttgatc ggcttggctc
     2881 tcccgctttt agtacacttt caagccagtg gagagcactt tatcgaaatc atcaatatgg
     2941 atgttgtagc tgggcttttg gtcaaagtcc ttgcagcctg tgtcggcctt atggcagcca
     3001 tcccagcaac ggcgatcagt gcagcatact tcaaaaagaa agtcgccaaa gccgatctaa
     3061 tcgatttgac atagcataaa gtagcagtag cgatctcagc gctacagcca cgcatgttga
     3121 aaacgtcata aacaagctat gataa
//
LOCUS       DAOSDA010000201         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_19839_length_3143_cov_2.801166, whole genome shotgun sequence.
ACCESSION   DAOSDA010000201 DAOSDA010000000
VERSION     DAOSDA010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19839_length_3143_cov_2.801166"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1016
                     /gene="flgA"
                     /locus_tag="PLW82_05395"
     CDS             18..1016
                     /gene="flgA"
                     /locus_tag="PLW82_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOJ46742.1"
                     /translation="MLRHSCWTGHIPRPIWILALILVLTGASFALTLKETAEVTGDQI
                     YMKDIIEEADDQPWAEISVLAAPAKGSKRPVYKTFVVTVLRHNGFLEEAQSLDGPNKV
                     DVLRLAEPKTEEAVIREVDHSWIWDEVKEEISAYISRLVPEGFAVRLTLVSFPQIAEK
                     ENVVDLSVDFGAFRNTFYGRMVVPVHLVFESGSTRRILISVDIAFEGEVLVAKMELRP
                     KDQLDELRVKKEVQVFTLNPNDCFFPSYELEKYQAKNLLRPGDIILKSSIEMVPVKLK
                     GETTKLYYKSGSIYVEISVVLLEDAVVGKMVRARNLASNAEVQGVVQEDGTLALKL"
     gene            1043..1642
                     /locus_tag="PLW82_05400"
     CDS             1043..1642
                     /locus_tag="PLW82_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOJ46743.1"
                     /translation="MRVSFPNKRSTARLFLVFLICFSLAVFASSESLWKLSNYSFFKN
                     TKEYAIGDLITVVVSERTEASQNASSSGSKDGSVAVNPGGGLLNFIPLISASGELEHS
                     ASGSMSKDNMFQTSITTKIVEILPNDIFKIEGRRIIKIDGEEQEIVLSGYIRKEDIST
                     NNTILSIYLADAQIELIGSGAINNKTNPGLISRVLEWLF"
     gene            1655..2770
                     /locus_tag="PLW82_05405"
     CDS             1655..2770
                     /locus_tag="PLW82_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOJ46744.1"
                     /translation="MNRARKLAPILLVLCLLSSLVLGQTEGQPEARIKELAGIVGVRS
                     NVLTGLGVVVGLNGTGDSSRYVAGSAIMAATLQKYGLEISSRDLASRNIAVVTVTAVL
                     PPFAREGDMIDVQVASIGDAKSLQGGYLMLTPLLAGDAIYALAQGSVSTGGYSVSSGS
                     SLSSRNQSVVGRVPNGAIVEQSVDVNYDFGDTITLSLFNKDFTTASRIAYVINESVGG
                     GAVSKDAGTVEVRIPEAYRDDPVAFVSLIENLRVVPDSVAKVVINERTGTVVFGEDVK
                     ISSVAVASGGLKVEVRAQTNVSQPLPLSDGQTVAYQNSDIFAEEETAGTFVLESTTSV
                     GDLVEALNAIGATPRDIISILQAMKEAGALHGVLEIM"
     gene            2823..3125
                     /locus_tag="PLW82_05410"
     CDS             2823..3125
                     /locus_tag="PLW82_05410"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOJ46745.1"
                     /translation="MSKSVSLGGKQYTDEDLMNACREFETLFTQELLKTMWATVPESE
                     LIKESQASKIWKDMYIEQLAKVSSQGRGLGVAETLYNQLRASQTYKKGSVAKLLNK"
BASE COUNT          840 a          701 c          849 g          753 t
ORIGIN      
        1 gataggtgtg cctctagatg ttaaggcata gttgttggac ggggcacata ccccgtccaa
       61 tttggattct agcacttatc ttagttctaa ctggtgcttc ttttgccctc actctgaaag
      121 agacggcaga ggttaccgga gaccagatct acatgaaaga cattatcgaa gaggcggacg
      181 atcagccatg ggctgagatc tcggtgcttg ccgctcctgc caaagggagt aaaaggcctg
      241 tttacaaaac ctttgtggtt accgtcctca ggcataacgg tttcctcgaa gaagctcaaa
      301 gcttagacgg accgaacaaa gtagacgtcc tgcgcctggc agaaccaaaa accgaagaag
      361 cggtaattcg agaagtcgat cacagctgga tttgggatga ggttaaagag gagatcagcg
      421 cttatatctc caggttggtt cctgagggtt ttgcggtgcg ccttactttg gtaagcttcc
      481 cacagatagc agagaaagag aacgtagtgg atctcagcgt ggactttggg gctttcagga
      541 acacatttta cggccgcatg gtcgtgccgg tgcaccttgt gtttgaaagc ggttccacca
      601 ggcgcatcct catctctgtg gacattgctt ttgagggaga ggtcctggtt gcaaagatgg
      661 agctcagacc caaagatcag ctggatgagc tgcgggtgaa aaaagaagtt caggttttta
      721 cgctgaaccc taacgactgc tttttcccaa gctatgaact ggaaaaatac caggccaaga
      781 acttactgcg tccgggagac atcattctaa aaagcagtat agaaatggtt ccggtaaagc
      841 tgaagggaga aacaaccaaa ctctactaca agtccggaag catctatgta gagatcagtg
      901 tagtgctcct ggaggatgcg gttgtgggga aaatggttag agcaaggaac ctagccagca
      961 atgcggaggt tcagggcgta gttcaggaag atggaacttt agcgctgaag ctttaagtta
     1021 aaatacacgg tggtgaagtg tgatgagagt atccttccct aacaagcggt caactgcaag
     1081 gctatttttg gtctttttga tctgcttttc actggcagtg tttgcctcaa gtgaatctct
     1141 gtggaagctc agcaactaca gttttttcaa aaatactaaa gagtacgcaa tcggcgacct
     1201 gatcacagtc gttgttagcg agagaacaga ggccagccag aatgcatcgt ccagcggctc
     1261 caaagacggt tcggttgcag tgaatcccgg cggaggactg ttgaacttca ttccgctgat
     1321 cagtgcctct ggagaactgg agcacagcgc ttcaggttct atgtcaaaag acaacatgtt
     1381 tcaaaccagc atcaccacga agattgtgga gattctgcct aacgatatct tcaagattga
     1441 gggcagacgg atcatcaaga tcgatggcga agagcaggag atagttcttt ctggttatat
     1501 ccgcaaagag gatatctcca ccaacaacac cattctctct atctatcttg cagatgccca
     1561 aatcgaactg atcggaagcg gcgcaattaa caacaaaacc aatccaggtc taatcagcag
     1621 agtgttagaa tggctattct agggggtttt tgtcttgaac agggcacgta aactagctcc
     1681 gattcttctg gtactgtgcc ttttgtccag tctggttcta ggtcagactg aaggccagcc
     1741 tgaagcgcgc atcaaagaac tggcgggaat tgtaggagtt cgctccaacg ttttgacggg
     1801 actgggagtg gttgtcggct tgaacggtac cggcgactca tcacggtacg tcgcaggctc
     1861 tgccattatg gcagctactc tccagaaata cgggttggag atcagctcaa gggatcttgc
     1921 cagcaggaat attgccgtgg tgacggtaac tgcggtacta cctccctttg ccagagaagg
     1981 cgacatgatc gacgtgcagg ttgcatctat tggtgatgcc aaaagcctgc aaggtggcta
     2041 cttgatgcta acaccgctgc ttgcaggaga tgccatctat gcactagccc aagggtctgt
     2101 gtctactggc gggtactcgg tgtcgagcgg aagcagtctt tcctctagaa accagagcgt
     2161 tgtggggcga gttcccaacg gcgctattgt tgagcagtct gtagacgtga actatgattt
     2221 tggcgacact attactttga gcctgttcaa caaagacttc acgactgcaa gcaggatcgc
     2281 atacgtgatc aacgagagtg tcggtggagg cgctgtgtcg aaagacgcag gtaccgtaga
     2341 ggttaggata ccagaagcct acagagacga tccggtagct tttgtttctc tgatcgaaaa
     2401 cctccgggtt gttccggact cagtggccaa agtagtcatc aacgagagaa cgggcactgt
     2461 ggtcttcggt gaagacgtta agatcagttc tgtagctgtt gcctcaggcg gactaaaggt
     2521 ggaggtaagg gctcaaacca atgtttccca accccttccc cttagcgatg ggcagacagt
     2581 ggcctaccaa aacagcgaca tcttcgccga agaagaaaca gcaggcacat ttgttctgga
     2641 gtcaactacc tctgttgggg acctagttga agctctgaat gctatcggtg caacgccaag
     2701 agacatcatt tctattcttc aagctatgaa ggaagcaggt gcccttcacg gcgttttgga
     2761 gataatgtga gggttgcttt atggagataa ctagttcgaa aaacattagc atggtttcga
     2821 tattgagcaa atcagtgtct cttgggggca agcagtacac tgatgaagat ctgatgaatg
     2881 cgtgcaggga gtttgaaacc ctgtttaccc aagaactcct caaaaccatg tgggctaccg
     2941 tacccgagtc ggaactgatc aaagaaagcc aggcgtcaaa gatctggaag gacatgtaca
     3001 tcgagcagct ggcaaaggtc agttcccagg ggcgaggtct cggagttgcc gagactctat
     3061 acaaccagct tagagccagt caaacctaca aaaaagggag tgttgcaaaa ctactaaata
     3121 agtagggagc aacgctccct ttt
//
LOCUS       DAOSDA010000202         3133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_19921_length_3133_cov_3.070500, whole genome shotgun sequence.
ACCESSION   DAOSDA010000202 DAOSDA010000000
VERSION     DAOSDA010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19921_length_3133_cov_3.070500"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="PLW82_05415"
     CDS             complement(<1..1256)
                     /locus_tag="PLW82_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46746.1"
                     /translation="MKRIAVIILVILVLSFIVSAEKVVLWTWYEGALGAILRDLINND
                     FTPTTGIEVEVLTVPIQDIVTKLIMAHIGGDAPDVVELYSSQVVELGVRGALLNLREY
                     PDIQSVIDQVYPQYLIGLSYRSALFGLPGENNWLLTYYREDLFNEFGLEAPVTWGDVR
                     TLSTKLLARDLGMYYSFVGDAITVNTNRLLPFLFQRGTDLYTPDGSGSNLDSPEAIAA
                     WIDYCNLYTEGKMLLEDPIETTFVSGDTPLFFGQSYRYSQIERIAPHLIGKWSIRELP
                     GTVKSTGEIDNTNSTTSLCWAIPASTKQKDAAFELLKWLTSSDFTGKFMLLANQSSEK
                     WRLFFATKDTLDNALFPEGHLEIVQRGLVNSRVPRAVIGGYVADRYVDFAFNKVVVQG
                     ADPEEAIREAASESTKEIQKKLKEFA"
     gene            1417..2070
                     /locus_tag="PLW82_05420"
     CDS             1417..2070
                     /locus_tag="PLW82_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ46747.1"
                     /translation="MPKPTFLNLSEEKRRRFVDVALKEFAEYEYRAASLNRIVAGADI
                     AKGSIYQYFENKKDLYQYLVTYAAREKFRFMALNLRENPPTLKAFLFDLFTLSRLFDF
                     QNPALGKLLLNTALEVENEELNGFLQEFRKLTASFYAEFAQGAAKRRFIRQDIDISFA
                     SFLLESFDKMICQYFEVKADRLYLDFVLDRRLSAEEKLEKIKSAIDSLVMALLGALV"
     gene            complement(2121..>3133)
                     /locus_tag="PLW82_05425"
     CDS             complement(2121..>3133)
                     /locus_tag="PLW82_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ46748.1"
                     /translation="SWGRFDLAIPIGRKVLWDMGQGNLYDLTLHYRDDGRSSDTVQTY
                     FGLRDIEIGDGRVFLNGRPIFQRLVLDQGYYKDGIYTAQDEALLRRDIELSMQLGFNG
                     ARMHQKVFEPRYLYYADKLGYLTWAEFGSWGLNLSDTGWKNIVPQWVEAVKRDRNHPS
                     IITWCPLNETYDGQDRELLVALYRLTHDLDGTRPVIDTSGYIHVVTDIYDTHDYVQDV
                     DEYVERYSRDELPINFPGLEKYEGQPFVVSEYGGMWWSGSEVESWGYGQRPKSVDEFM
                     ARFKGLTETLLNCEKVCGFCYTQLYDVEQETNGLLDYDRNPKFSFETISAILTQKAKI
                     EE"
BASE COUNT          750 a          813 c          756 g          814 t
ORIGIN      
        1 ctggcaaatt ccttaagctt cttttgaatc tccttagtcg attcggaggc agcttctctg
       61 atcgcctctt ctggatctgc accctgcacc acaaccttgt taaaggcaaa atctacgtag
      121 cgatcggcaa cgtagcctcc gataacagct ctaggtacgc ggctgttgac aagtcccctc
      181 tggacaatct ccaaatgccc ttcgggaaag agcgcgttat ccaaggtgtc tttggtggcg
      241 aaaaacaagc gccacttctc cgaggattgg ttggcaagga gcatgaactt gccagtaaag
      301 tcgctggaag tcagccactt aagcagctca aacgcagcgt ccttttgctt tgtggatgcg
      361 ggaatcgccc agcacagcga ggttgtggag ttggtgttgt cgatctctcc agtgcttttg
      421 actgttcccg gaagctctct aatagaccac ttgccgataa gatgcggcgc gatcctttca
      481 atctgtgagt agcggtagct ctgtccgaag aaaagtggtg tgtcgccgga cacgaaagtg
      541 gtttcgatag ggtcttctag aagcattttc ccttcggtat acaggttgca gtagtcgatc
      601 caggctgcaa ttgcctcagg tgagtcgagg tttgaaccgc tgccgtccgg agtatagaga
      661 tctgttcccc tctggaacag aaacggcaaa agccggttgg tgtttacggt gatggcatcg
      721 cctacgaagc tgtagtacat ccccagatcc cgggccaaga gcttcgtgga cagggttctg
      781 acatcacccc atgttacagg cgcttccaga ccgaactcgt taaaaagatc ctctctgtag
      841 taggtaagga gccagttgtt ttctcctggc aggccaaaca atgcgcttct gtaagaaagc
      901 ccgatgaggt actggggata aacctgatcg atcacgctct ggatgtcggg gtactcccta
      961 aggtttagga gagcacctct tactcctaac tctacaacct ggctggagta gagctccacc
     1021 acatcaggag cgtcgccgcc gatgtgggcc atgatcagct tggtcacaat atcctgaatc
     1081 ggcactgtca agacttccac ttcgatccct gtagtaggtg tgaaatcgtt gttaatcaga
     1141 tctcggagga ttgcaccaag ggccccttcg taccacgtcc agagaaccac tttctccgca
     1201 gaaacgataa agcttaatac caaaataacc aaaatgatta cagctatcct cttcacttta
     1261 cacccctccc ataagttcta cttatttttt acagtattgg tcggcaattt ccttttttca
     1321 cttctcgttg gaaaaagctg cccaaactaa actactatgc taagagttcc attatttggt
     1381 atacttaagt aaaagatctg cagagaggtg acttctgtgc ccaaaccaac gtttctgaac
     1441 ctcagtgagg aaaaacgcag acggtttgtc gatgtagcgc tcaaagagtt tgcagagtat
     1501 gagtatcgag cagcctctct caaccgcatc gtggctggtg cggatattgc caagggtagc
     1561 atctaccagt atttcgagaa caagaaggac ctctaccagt acttggtaac ctatgctgca
     1621 agagagaagt tccgctttat ggcacttaac ctccgagaaa atccacccac tttgaaggct
     1681 ttcctctttg accttttcac tctcagcagg cttttcgact tccaaaaccc cgccttaggt
     1741 aagctgctcc tgaacaccgc cctggaagtg gaaaacgaag agctgaacgg ctttctccag
     1801 gagttcagga agttgaccgc aagtttctac gcagagtttg cccaaggtgc agcaaaacgc
     1861 cgctttatcc gccaggacat cgacatctcc ttcgctagtt tccttttgga gagcttcgac
     1921 aagatgatct gccagtactt cgaggtcaag gcggacaggc tgtacctgga ttttgtcctg
     1981 gacaggagac tttcagctga ggaaaagctg gaaaagatca aatcggcaat cgacagtttg
     2041 gtgatggccc tccttggggc tttggtataa aatcatttat atgtccacga caaagagacc
     2101 tccaagcgga gatctctttt ttactcttct atcttggcct tttgagtcag gattgcagaa
     2161 atggtctcaa aagaaaactt ggggtttcta tcgtagtcta gcaggccgtt tgtctcctgc
     2221 tctacatcgt agagctgagt atagcaaaaa ccgcaaacct tttcacagtt caggagggtt
     2281 tccgtaagac ctttgaacct tgccataaac tcatctacag atttgggccg ctggccgtag
     2341 ccccagctct ctacctcaga gccgctccac cacatgccgc cgtactcgct gaccacaaac
     2401 ggctgacctt cgtatttttc gaggcccggg aagttgatcg gcagttcatc cctactgtag
     2461 cgttcgacgt actcgtccac atcttgtacg tagtcgtgag tgtcgtagat gtctgtaaca
     2521 acatgaatgt agccgctggt gtcgattact ggcctggttc cgtctaggtc gtgtgtcagg
     2581 cggtacagtg ccaccaggag ctccctatcc tgaccgtcat aggtctcgtt caatggacac
     2641 caggtgatga tcgacgggtg gtttctgtcc ctttttaccg cttccaccca ctgaggaacg
     2701 atattcttcc aaccggtgtc tgacagattc agaccccaac taccaaactc ggcccaagtg
     2761 aggtatccca gcttatcggc gtagtagagg tacctgggtt cgaagacctt ctggtgcatt
     2821 ctagccccgt tgaaccccag ctgcatggac agctcaatgt cgcgtctgag aagcgcttca
     2881 tcttgtgccg tgtagattcc atctttgtag tagccctggt cgaggacgag cctctggaag
     2941 atgggcctgc cgtttaggaa aacccttccg tcgccaattt caatgtctct gaggccgaaa
     3001 taggtctgaa cggtatcgct ggatctacca tcatctctgt agtggagggt caggtcgtag
     3061 agatttcctt gtcccatatc ccaaaggact tttcttccaa tgggaatggc aagatcaaac
     3121 cttccccagg aaa
//
LOCUS       DAOSDA010000203         3108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_20146_length_3108_cov_2.861775, whole genome shotgun sequence.
ACCESSION   DAOSDA010000203 DAOSDA010000000
VERSION     DAOSDA010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20146_length_3108_cov_2.861775"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="PLW82_05430"
     CDS             <1..1421
                     /locus_tag="PLW82_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ46749.1"
                     /translation="AYPGGRGSDQLVVYTRDYGKPTTGTNPYGTEFVVAGGIVTEFGS
                     NNSRIPEDGFVVSGHGEAQNWLQENLWYGSRVYLENDEIKVLTDETTERDGARILAEK
                     MAEEGILSSSSAAPTTRSEIEDLYLKSLPSRTVEVRGIWHEQTEKTPNEIRKTVKRLQ
                     DSGFNLLLLEVYYHGYTIYPSKFATQMPQYEGWDPLAVWQEECNKAGIEIHPWVHCFF
                     AGFGEKGSVADLHPEWLGKRRDGKLQSNVERGFYWLEPALDEVQDFLISLFKELLHTY
                     GFSALHLDYIRYGETACFNEAFSFSDSAVKVFKEKTGLDALELTKDSQEWALWLKFRE
                     DNITRFVRRARKEFPGVLLSTAIFPELEHARASKGQNWALWLEEGLLDYLAPMIYSTD
                     TEYVVKMTEFAKDLNPQIPVYTGLGPFMGFSPLELARQIYAVSKAAPGTVLFALHSVK
                     DRHMEALKKGIFKAKAVLPWQK"
     gene            complement(1559..2104)
                     /locus_tag="PLW82_05435"
     CDS             complement(1559..2104)
                     /locus_tag="PLW82_05435"
                     /inference="COORDINATES: protein motif:HMM:NF027050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HOJ46750.1"
                     /translation="MKATGIVRRIDDLGRVVVPKELRDSLKITEGDTIEIFTAEDGEI
                     VLKKYSPMAELSDVMTSYAQTLSSAAGASVIITDKDTVVATSNVDMDINQRPVGDRVK
                     QVFDERRTIAFKSNAPSEVVDYPEFDNFKSRNFIVTPVISPAGDFLGTVILASESELS
                     DFESKIAETAASFIAKQVEVD"
     gene            2372..>3108
                     /locus_tag="PLW82_05440"
     CDS             2372..>3108
                     /locus_tag="PLW82_05440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ46751.1"
                     /translation="MHVLLVNILFQGGQTTHTIELAVGLKRKGVKVSFMLIGEKAYPR
                     LAKEFRKIMAENGIPVFTRSDAKTLLPNVDIVHAHSSWTYALAVRWKEIYGVPVVFTI
                     HGLGIRADQRLKKANALIAVGRRTKEGLPDELQGKTVLITNGVDLEKYKPPPYKAPSK
                     PLIVLYAGRLTEKKVPGLEALAAAVQKAGLLLLVASDREVPLGRNAVFVGWQEELLPI
                     MQQVDVLFACGRTIREGMALGLACGVLG"
BASE COUNT          783 a          732 c          846 g          747 t
ORIGIN      
        1 cagcatatcc aggagggaga ggtagcgatc agctggtggt ctacaccaga gactacggta
       61 aacctactac cgggaccaac ccatacggta ccgagtttgt ggtggcaggt ggcatagtga
      121 ctgagtttgg ttcaaacaac agccgcatac ctgaagacgg gtttgtggtc tccggacacg
      181 gagaggccca gaattggctg caggagaacc tctggtacgg atcgagagtg tacctggaaa
      241 acgacgagat caaggtctta accgatgaga cgactgaaag agacggagca aggatcttag
      301 cggagaagat ggcagaagag gggattcttt ccagctcgag cgctgctcca accacaagat
      361 cagagatcga agacctgtat ctgaagagtc tgccgtccag aaccgtagag gtacggggta
      421 tttggcacga acagacggaa aaaaccccaa acgagatcag gaaaacggtg aagaggctgc
      481 aggacagcgg cttcaacctg ctgcttctag aagtctacta ccacggctat accatctacc
      541 cgtcaaagtt tgccacccag atgcctcagt atgagggctg ggatccacta gccgtgtggc
      601 aggaagagtg caataaggca gggattgaga tccatccgtg ggtgcattgc ttctttgcag
      661 gcttcggtga gaaggggtct gtggccgatc tccacccaga gtggcttggg aaaaggcgag
      721 acggcaaact ccagtccaac gtggagcggg gcttctattg gctggagccg gctcttgacg
      781 aagtccagga cttcttgatc tccctgttca aagagctttt gcacacctac gggttttcag
      841 ccttgcactt ggactacatc cgctacggcg agactgcctg tttcaatgag gctttcagct
      901 tcagcgacag tgcagtgaag gttttcaaag aaaagaccgg tctcgatgct ctggaactta
      961 ccaaagacag ccaggagtgg gctttgtggc tgaagtttcg tgaagacaac atcacccgct
     1021 ttgtccgccg ggcaagaaaa gagtttccag gtgttttgct gtctacagcg attttcccag
     1081 agctcgagca cgcaagggca agcaaagggc agaactgggc cctttggctg gaagaaggac
     1141 tgctggacta cctggcacct atgatctact ccaccgatac tgagtacgta gttaagatga
     1201 cggagtttgc caaagacctc aatccccaga tcccggtcta caccggtctt ggacctttca
     1261 tgggtttttc tccgttggag cttgcaaggc agatctatgc agtctccaaa gcagcgcctg
     1321 gcacggtgct gtttgccctc cattcggtga aggacaggca catggaggct ttgaaaaagg
     1381 gcatttttaa agcgaaggca gtattaccct ggcagaagta gggtggagag ctaaaccgaa
     1441 accgattgca acctaaaaaa acgagcaggc agcgatttgc ttttttaaga cagacagttc
     1501 actaggtcaa cggtctgtca aagttaaagc tcaaagcgcc tgctctttgt ttttgggttt
     1561 agtccacttc aacttgtttg gcgataaagc tagcagcggt ttcggcgatt ttcgattcaa
     1621 aatcggagag ttcgctttca gaggcaagga taaccgtgcc gagaaaatct cctgctggag
     1681 agattaccgg agtcacaatg aagtttcttg atttgaaatt atcgaattcg ggatagtcta
     1741 ctacttcgct gggcgcgttg ctcttgaatg cgatggttct ccgttcgtca aagacctgct
     1801 tgacccggtc accgacaggc ctctgattga tatccatatc cacattgctg gtagccacaa
     1861 cggtgtcttt atcggtaatg atcacactgg ctccggcggc actgctcagc gtctgtgcgt
     1921 aactggtcat cacgtcgctg agctcagcca tcggagagta tttcttaaga actatctcgc
     1981 cgtcttctgc ggtgaatatt tcaatggtgt cgccttcggt aatcttcagt gaatcccgga
     2041 gctctttagg tacgacgact ctgcccaggt catctattct acgcacgata cctgtcgctt
     2101 tcatttgagg agtcttcctt tctgatggag tgctttgttt ttagttttag catccctaga
     2161 aaaaacatac cagccgaaga cagtggtaaa tctatgtaac ctgatatgca caccagcgga
     2221 gtgtgacgag gttttgcagc agatcgctca agtataagtg cggctgtaaa aatagtttgg
     2281 ctcaatttcg gggctctaat acatcggaag aagacctcag agccatacac tcccggaaaa
     2341 ggattgcata gcctttagag aggtgctggt tatgcatgtt ttactggtta acattctctt
     2401 tcagggagga cagaccacac acaccataga gcttgcagtt ggacttaaac gcaagggtgt
     2461 aaaggtttca tttatgctta tcggcgagaa ggcctatcca cgccttgcca aagagtttcg
     2521 caaaatcatg gcggaaaacg ggattccggt gttcacaaga tctgatgcaa agacacttct
     2581 ccctaatgtg gatattgttc acgctcattc ctcctggact tacgctttag cggtgcgctg
     2641 gaaggagatc tacggcgtcc ctgtggtttt taccattcac ggacttggca taagggcaga
     2701 tcagaggctt aaaaaagcca acgctctgat cgcagttggc agaaggacca aagaaggcct
     2761 cccagatgag ctccagggga agacggtgct gatcacaaat ggagttgatc ttgaaaagta
     2821 taagccccct ccatacaaag cgccgagcaa acccctcata gtgctctatg ccggaaggct
     2881 caccgagaaa aaagtgcctg ggctcgaagc tttggcagct gcggttcaga aagcaggctt
     2941 actacttctt gtggcttcag atagagaggt tccgctgggg agaaacgccg tttttgtggg
     3001 gtggcaggaa gaacttttgc caatcatgca gcaggttgac gtgctttttg cctgcggcag
     3061 gaccatcagg gaaggaatgg ctctcgggct tgcctgtggc gttttggg
//
LOCUS       DAOSDA010000204         3106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_20166_length_3106_cov_2.782039, whole genome shotgun sequence.
ACCESSION   DAOSDA010000204 DAOSDA010000000
VERSION     DAOSDA010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20166_length_3106_cov_2.782039"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..685)
                     /locus_tag="PLW82_05445"
     CDS             complement(242..685)
                     /locus_tag="PLW82_05445"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HOJ46752.1"
                     /translation="MSLVESFSLDHTQVQAPFVRLAGRYRGPKGDVISKFDLRFVQPN
                     QGAIPTGAMHTLEHLLAISFREEMEGVIDVSPMGCRTGFYFLVFGEVDSHAVLDAFKN
                     VLEKVIAAKEIPAATAVQCGNFQDHSLSGAIEYARKALDGLHVHE"
     gene            847..1350
                     /locus_tag="PLW82_05450"
     CDS             847..1350
                     /locus_tag="PLW82_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOJ46753.1"
                     /translation="MNEQIPEAITKALEFERSGAIFYLETAAKIKSRLARRLLHNLAM
                     EEIQHLQDIEGVCQDIEDEKIPKELDKDTREIIEGTIKSLFKVKKDELSEDATDLDVL
                     KVAMDVEEKGYKMYVEAGEASDNLSLKEFFNRLAEEEQDHMAALKNVYHYLSDPEDWL
                     AQAESQL"
     gene            1563..>3106
                     /locus_tag="PLW82_05455"
     CDS             1563..>3106
                     /locus_tag="PLW82_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ46754.1"
                     /translation="MDRKPKVFLVLLAIALFSILVSASFLPDRINQLQREVANFQRSI
                     DEARLQMKRLDYDYLEGIVASLTTKLEQANELVAADEDADEAYVLVADITNFLKESVV
                     HLHESRPIETRGMWMDNFTIDSFYTREDVAKHMDQLKAINVNVIFPDVYGNGAAIYPS
                     KVVPMHDRFKTVFQDEDVLTVIIDEAHKRGIEVHPLVRVFALHNGVRYFVDDKVHWLD
                     LTSENRFTLDNGYYWLSPSLPEVREYLMSFLKELTENYDIDGIHLDYIRFDDNFGYNK
                     HTRDLFKAFNGVDPFDINSTRLADEFKYFKSEFVNSFVERVYKETKAIKPDLLISAAV
                     ASPYSWGKNDLGQDWLSWALNRYINFMTPMSYRSTADSYQSTVKSELTTVGGATLIFT
                     GMGVYLYDEYVLLEQLEAGRFTNMSGQSLFSTANMKVNDYIFLQNGPYKEPAVPTFRD
                     PQHAAGLLAKDLIARINEFKPFFDEKANAALDSYLAEIEDISAKIDALELRPWDTRDL
                     QEGSPE"
BASE COUNT          894 a          725 c          701 g          786 t
ORIGIN      
        1 caatacaaaa gggctttgta ttgcaatctt gctttgttca gttttcaagg ttctttacgc
       61 tgccgcctca ccggcgacat ttattattct aacagcgcta caaacgaaag tcaaccgcat
      121 tctagcgtaa tttttaattc aaattaacgt tgcagtcaga aagctgctcg gtcttctgtt
      181 ttggctggta gaagataaat gacttcgtaa agactggaag ctttccggct ttatttaacc
      241 cctactcatg cacatgcagc ccatctagag cttttctggc atactcaatt gcaccgctca
      301 gggaatgatc ctggaagtta ccgcactgaa cagcagttgc tgcagggatc tctttcgccg
      361 cgatgacttt ctcgagcacg ttcttgaaag catcaagaac cgcatgagaa tctacctctc
      421 cgaacacgag gaagtagaaa ccggttctgc atcccatagg agaaacatcg ataacgcctt
      481 ccatttcttc acgaaaggaa atcgcaagaa gatgttccaa ggtgtgcatt gcgcctgtag
      541 ggatggctcc ctgattaggt tgaacgaacc gcagatcgaa cttgctgatc acatcacctt
      601 ttggtcctct gtaccttcct gcgagcctta caaaaggtgc ctgcacttgg gtgtgatcta
      661 gtgagaagct ttctaccaaa ctaatatcca ttaccattcc tccttggcgt atgattatct
      721 taccacattt accgagggct aaaccatgaa attgtaaaag actcctgttg ttttttctta
      781 gacttcaaag ggtatttgaa aggaagtaca tcaacgtttt ttaaaagggg caagggaggg
      841 tttttagtga acgaacagat accggaagca ataaccaaag cactggaatt cgaaagaagc
      901 ggtgccatct tctatttaga gactgcagcc aaaatcaaaa gccgcttggc aaggcgactc
      961 ttacacaacc tagctatgga agagatccag caccttcagg acatcgaggg agtctgtcaa
     1021 gacattgagg atgagaaaat tcccaaggaa ctagacaaag acacccgaga aataatagag
     1081 ggcaccatca aatcgctctt caaagtgaaa aaagacgagc tcagcgaaga tgccactgac
     1141 ctcgatgtgc ttaaggttgc catggatgtg gaagagaaag ggtacaaaat gtatgttgaa
     1201 gcaggggaag cttcggacaa tctttctcta aaagaattct ttaacagact tgctgaagaa
     1261 gaacaggacc atatggccgc tcttaaaaac gtctaccatt acctttccga ccctgaagac
     1321 tggctagctc aagctgaaag tcagctttag gttcggacgc tgacttgatc gcccgatgct
     1381 tagtcctaaa tttacaaaat gccaaaaggt accgaacatc tgaatgcctt ctcaccagct
     1441 tgggaaggca atttctcact taacagcgat tttccctgag caggattaaa ctaatagtgt
     1501 agagaaaaac tctttttgta ggaattatca agattattag ggggaagggt aaggtgatta
     1561 ctatggatag aaaaccaaag gtttttctag ttctccttgc aattgcactc ttttcaatcc
     1621 tggtttcggc aagctttttg ccagatagaa tcaaccagtt gcaaagagaa gttgcgaact
     1681 tccaaaggtc aattgacgaa gcacggcttc aaatgaagcg tttagactat gactatctcg
     1741 aaggaattgt cgcttcatta accactaagc tagaacaggc aaacgagcta gtggcagctg
     1801 atgaagacgc agatgaggcg tatgttctcg ttgctgacat taccaacttc ctgaaggaat
     1861 cggtggtgca tctccatgag tcccgcccca tcgagacgcg tggcatgtgg atggataact
     1921 tcaccatcga ctcgttctat accagggaag acgttgccaa gcatatggac cagctcaaag
     1981 ccattaacgt aaacgtgatt ttcccggatg tttacggcaa tggtgcagcc atctatccaa
     2041 gcaaagtggt cccgatgcac gacaggttta agacggtttt ccaagacgaa gacgttctca
     2101 ccgtgattat cgacgaggca cacaaacgcg gtattgaggt gcaccctctg gtgagggtct
     2161 ttgcccttca caacggtgtg cgctactttg tggacgacaa ggttcactgg ctggacctca
     2221 ccagcgagaa ccgcttcaca cttgataacg gctactactg gttatctcca tctcttccag
     2281 aggttagaga atacctgatg agtttcttga aagagctcac agaaaactat gacatcgatg
     2341 gcatccatct cgactacatc cgtttcgacg acaacttcgg ttacaataag cacaccagag
     2401 atctttttaa agccttcaat ggcgtagatc cgttcgacat caacagcact aggctggcag
     2461 acgagttcaa gtacttcaag agcgaattcg ttaacagctt tgtggaaaga gtctataaag
     2521 aaaccaaagc tataaagcca gaccttttga tctctgcagc agttgcctct ccttacagtt
     2581 ggggcaaaaa cgatctggga caagactggc tcagttgggc cttaaacaga tacatcaact
     2641 tcatgactcc gatgtcctac cgttctactg ccgatagcta ccagtctaca gttaagtcag
     2701 agttgactac agttggtgga gcaacgctta tctttaccgg catgggcgta tacctctacg
     2761 acgaatatgt cttgttggag cagcttgaag caggccgttt taccaacatg tccggtcagt
     2821 cgctcttttc cactgccaac atgaaggtta acgactacat cttcctccaa aacggaccgt
     2881 ataaagagcc ggctgtaccg acctttagag atccccagca cgcagctgga ctccttgcaa
     2941 aggacctaat agcgaggatt aacgagttca agccgttctt tgatgaaaaa gccaatgcag
     3001 ctttagatag ttatcttgcc gagattgaag acatctccgc gaagatcgat gctctggagt
     3061 tgagaccttg ggacaccaga gatctccagg agggaagccc cgaaga
//
LOCUS       DAOSDA010000205         3085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_20366_length_3085_cov_3.196370, whole genome shotgun sequence.
ACCESSION   DAOSDA010000205 DAOSDA010000000
VERSION     DAOSDA010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20366_length_3085_cov_3.196370"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..1131
                     /locus_tag="PLW82_05460"
     CDS             394..1131
                     /locus_tag="PLW82_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOJ46755.1"
                     /translation="MLVDILVEFLKGFGIFVGYYAGAVSLLLMFRRFLNPPKEIVRKA
                     FHLICAMSALVLLYAFETWYLAVAAALTLAVALFPIFTYLDRFPKVMEVLVQRTKGEI
                     RSSLVIVYGMMAVLITLFWGALGPGWKYIVVVSIMAWGFGDAAAALVGKAFGKKQIIH
                     RLVEGSKTREGTIAMYAVSGFAIFVTLLICTSFPWYLCLVGALLVAPICALVELISKR
                     GLDTITVPFATAIPLFLLAVFFNFFGL"
     gene            1474..2865
                     /locus_tag="PLW82_05465"
     CDS             1474..2865
                     /locus_tag="PLW82_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOJ46756.1"
                     /translation="MSTHKEVLGYLPDERPPVLELIFFALQQIVVMFPATVLVALITG
                     FHVSTTIFASGLATLAFILITGRKIPLYYGSSFSYIAAIASIMSAEAFAGYTLNERIS
                     IAQFGIVMSGLVSIAAGFVVNKGGQKLIDKVLPPTVTGSIAIIIGLSLAKNALLNAAA
                     TPAGFESSTLAVNGAWIIALITLLSTILYSVYLKGKLSQFPILFGLATGYVAALIVGA
                     VTKVPFVDFNTLNTAKVFSLPVFTLPKPNLAALIAIMPIALATIPESTAHIFQLDIYV
                     KNLAKRKGSRKEYNIDQKLGLNLIGDGIGDIVSGFIGGPAGTNYGENISAMAITRNFS
                     VDVLIAASVITMLISCFTPLINIVYSIPTAVIGGLSIYLFGVIGAQGIAIIMEKKVDM
                     FSAKNLAVIAVILIVGLGGAFLQDNGMIPMFGVELPALATASIVGILLNLALSVGDKG
                     TEEDEYKEKSVSI"
BASE COUNT          721 a          738 c          753 g          873 t
ORIGIN      
        1 cgcgaaagcc ttggattgtt ggaatagtca agtccaaaaa aacgtccgca cccccaattc
       61 tctccatttt attatgccac tcacaatcaa tggacttctt tatttagaaa agcccctctt
      121 tattttcgct tttgaaataa caatggttgt atgatctggt ccagaaactc ctgtgtttag
      181 tgggtttctg gatctcgctt tttcttaaat tatgtgaaaa gtctgattat ctttaggcag
      241 atatgcatga aaaacgaaag ctcaagtata ctttaacaga cgactccaaa gcgtcctgcc
      301 cctgttccat caggtgtgaa atactgttat actgggtata ggtgagacag agagtccagc
      361 cttgaacgtc ccgtaagcag aggaggtctg caaatgctcg ttgacatcct ggttgagttt
      421 ttgaagggat tcggtatttt cgtaggctac tatgcaggtg ctgtctcttt gctgctcatg
      481 tttagacggt ttttgaatcc gcccaaggag atcgtgcgca aggcatttca cctgatttgc
      541 gccatgtccg ctttggttct gttgtacgcc ttcgagacgt ggtatctggc tgtggcagct
      601 gctctaaccc ttgcagtcgc gctttttccc atatttactt atctggatag gtttcccaaa
      661 gtcatggagg tcttggtcca gcgcacgaag ggagaaatcc ggtcgagctt ggtcatcgtt
      721 tatgggatga tggcagtcct cattacgcta ttttggggcg ctctcggacc agggtggaag
      781 tacattgttg ttgtttccat catggcatgg ggcttcggcg acgccgcagc ggccttagtt
      841 gggaaggcct tcgggaaaaa acaaatcatc caccggcttg tggaaggttc gaaaaccagg
      901 gaaggaacca tcgctatgta cgccgtttcc ggctttgcca tttttgttac gctcttaatc
      961 tgcacctctt ttccttggta cctgtgcctt gtgggggcct tgttggttgc acccatatgc
     1021 gcgttggtgg aactgatctc gaagcgtgga ctagacacca tcaccgtacc ttttgccaca
     1081 gcaataccgc tgtttttgct ggcagtcttc ttcaacttct tcgggcttta agtgagccgc
     1141 agaaagcttg ttttgttcta tttccgctat gccagggact gagcgccgat gcttcatgga
     1201 cgttcgagtt tgttaccgga aatcgtttta taattaacag aaatatttaa aatagtgctt
     1261 ctttgttttt tgctgtttac aaaggagagg acctgtgcca taatggcagt ggtaaaagaa
     1321 atctttaaac ttggtacaga gataccagta agattcgtat attgaggcag cctaggggtt
     1381 ccacctcttt ttgtgctgtt gtcattgcaa tcttgctgga gggcaagatt tttttttgcc
     1441 tactccaaaa cgaaacattg aggaggaagc tttatgtcaa cacacaaaga agttttaggt
     1501 tatctgcctg atgaaagacc accggttcta gagctgatct ttttcgccct gcagcagatc
     1561 gtggtaatgt ttcccgctac agtattagtg gctctcatta ccgggttcca cgtctcaacc
     1621 acaatctttg ctagcggtct tgcaactctc gcgttcatcc tgatcaccgg gagaaagatt
     1681 cccctgtact acggttccag tttctcgtac atcgctgcaa tcgcctcgat catgagcgct
     1741 gaagcgtttg cgggctacac gctgaacgag cggatctcca tcgctcagtt cggtattgtg
     1801 atgagcggtc tggtttccat tgcagcgggc tttgttgtga acaagggtgg acagaagttg
     1861 atcgacaagg tccttccacc taccgttacc ggcagcattg ccattatcat cggcctttct
     1921 ctggcaaaga acgcactttt gaacgctgca gcaactccag cagggtttga aagtagcaca
     1981 cttgcagtga acggcgcttg gattattgcg ctaatcacgt tgctttcaac gatcctgtac
     2041 tcggtttact tgaaaggcaa gctcagccag ttcccgattc tattcggttt agccacaggc
     2101 tatgttgcag ctctgatcgt cggtgctgta acgaaggttc cgtttgtgga cttcaacaca
     2161 ttgaacaccg ccaaagtttt cagcctaccg gtgttcacac tgccaaaacc caatcttgcg
     2221 gccttgatag ccatcatgcc cattgcgctg gcaacaattc cagagtccac agcccacatc
     2281 ttccagctgg acatctacgt aaagaacttg gctaaacgca aagggtccag gaaagagtac
     2341 aacatcgacc agaagctggg ccttaacctg atcggcgacg gcattggcga catcgtttcc
     2401 ggattcatcg gcggtcctgc aggtacaaac tacggtgaga acatcagtgc gatggccatc
     2461 accagaaact tctcggttga cgttctcatt gccgcatcgg taatcaccat gctgatctcc
     2521 tgcttcacac ctctgatcaa tatcgtgtac tcgatcccaa cagcagttat cggcggactt
     2581 tccatctacc tgttcggagt catcggcgca cagggtatcg cgatcatcat ggagaaaaaa
     2641 gtggacatgt tcagtgccaa aaatctcgca gttatcgcgg tcatcctcat cgtcggtctt
     2701 ggcggcgctt tcctccaaga caacggcatg attccaatgt tcggagtaga gcttcctgcg
     2761 ctggcaacag catcaattgt gggcattctg ctcaacttag ccctttctgt aggggacaaa
     2821 ggcacggaag aagacgagta caaagaaaaa tcagtttcaa tctaagataa atactcaggt
     2881 gagccttgta aaagaggctc accttttttg ttctctgcta agtacttgac ttttgcaggt
     2941 gtgctataat tgtggcagga acgccgagtt cgggtccggt gcctgcagtt gtcaactaat
     3001 gcgctgcgac tggctgtcgg catatagctc ggatgaaggc agcgacttgt ctgccataaa
     3061 aggaggggag aagagcggaa atgga
//
LOCUS       DAOSDA010000206         3074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_20456_length_3074_cov_3.376284, whole genome shotgun sequence.
ACCESSION   DAOSDA010000206 DAOSDA010000000
VERSION     DAOSDA010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20456_length_3074_cov_3.376284"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLW82_05470"
     CDS             <1..499
                     /locus_tag="PLW82_05470"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOJ46757.1"
                     /translation="AVLRVLEQYGLKPQVKWPNDVFVGGRKISGVLAELSAIGPDIRH
                     IVLGIGLNVNQTEFSPDIPGTSLKIELQRELCRREVLAALLLHLEEGYEKLSQDVHWL
                     IEIMKKYSILLGERVTVTGGVELVGIAEDIASDGALLVRVVENGEERVVKVWAGDVSV
                     RKTEA"
     gene            complement(607..2145)
                     /locus_tag="PLW82_05475"
     CDS             complement(607..2145)
                     /locus_tag="PLW82_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ46758.1"
                     /translation="MNNELLRFVEPIYRFCAKRLSSFADAQDLAQEILLCVLQGINQS
                     KIANLDAYVWKVAHNRYGRKVNTENKDLVLVHGAEYVSDFAEDFDSSFDESSDEFQAV
                     FQALHTLSSTYRQILVEYYVHNLSVREISCKLGISAETVKWRLHVGREKVKERLSEME
                     RNYEKIKMSVMCNGSFAPTRYVNTQVHKAIAKACYDAPLTIEEISVCTGIPTIYLEEP
                     LEHMIYGDAIVQIGKKYATNFIITDIKDLESTYEYLSKPVIEEITDKLVAYITETEKI
                     WRGIGFYGSDFPLSHLYHVLIPTLVFQSGDRFRENSSILPKDRPPRKDGGNGWFIVIE
                     GVEELDDKFSGCNHYHYYSNTEPPAKFIYYWNGASYDYNLDNILRNARFFIYSIGPDN
                     ECVLVDDEDAAKALENGLCKKMWDRYYPAMPVLTSNEYQALQKWAAECHAIDAVWPKW
                     IEALYGSYKSTTPKRLADQIGGNVDSSAYNIVALITKELTSRGLADKPEGDQPFTKNL
                     LLVK"
     gene            2538..3029
                     /locus_tag="PLW82_05480"
     CDS             2538..3029
                     /locus_tag="PLW82_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013659141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ46759.1"
                     /translation="MFGENLKRLRKQKGLSQEELAERLHVVRQTISKWEQGRSVPDAD
                     LLIRIAEIFEVPVSTLLGDAVEVSNGTDLVAEKLEQLNLILAERNRRSRMIWKVVGVT
                     LISIAVLTVLGIITALIGLVAYTTVQSTTAEKTEVLVEPVEVEHPVPYELEALEEDLV
                     FIE"
BASE COUNT          829 a          667 c          776 g          802 t
ORIGIN      
        1 cgcggtgctc cgggttttgg aacagtacgg tctaaaaccc caagtgaagt ggcctaacga
       61 tgtctttgtc ggaggaagga agatctcggg agtccttgca gaactctctg ccatcggacc
      121 ggatatccgc cacattgtgc ttggcatagg gctaaacgtt aaccagacag agttttcccc
      181 tgacatcccc ggcacatcgc tgaagatcga gctgcaaaga gagctgtgca ggagagaggt
      241 tttggcggct ctcctcctac acttagaaga gggctatgag aagctttctc aggacgtcca
      301 ttggctgata gagatcatga agaagtattc gatccttctc ggggaaagag tgacagtaac
      361 aggaggagtg gagctagtag gcatagccga agacatcgca agcgacggag ctctcttggt
      421 gagagtagta gagaacgggg aagaaagagt cgttaaggtc tgggcagggg acgtaagcgt
      481 acgcaagaca gaagcataag gcatttggac taagctcatt tgtagaagtg ccggatgtgt
      541 atttcaataa aggttctagt tacgacgcaa aaaggcggga agagctttgg cttcccgcct
      601 tgatctttat ttcacaagca agaggttctt ggtgaacggc tgatcgcctt caggtttgtc
      661 ggctagaccg cgacttgtaa gctctttggt gatcagagca acgatgttgt aggcagaact
      721 gtctacattg cccccgatct gatctgcaag tctttttggc gttgtggact tgtagctgcc
      781 atacagcgct tctatccatt tcggccaaac ggcatcaatg gcatggcatt cagcagccca
      841 tttttgaagc gcttgatact cattggaggt aagtaccggc atggcagggt agtagcggtc
      901 ccacatcttt ttacatagac cattctccaa tgctttggca gcatcttcat catccacaag
      961 gacgcactca ttgtccggac ctatagaata gataaagaac ctagcattgc ggaggatgtt
     1021 atccaggttg taatcatagg atgctccatt ccagtagtaa atgaacttgg caggcggttc
     1081 tgtgttgctg tagtaatggt agtggttgca gccggagaat ttatcatcaa gttcttctac
     1141 gccttcgatg acgataaacc aaccgttacc tccgtctttg cggggaggac gatccttagg
     1201 caaaatggaa gagttctccc tgaacctgtc tccgctttga aaaaccagtg ttggaatcaa
     1261 gacgtggtac agatgagata gagggaagtc tgaaccgtaa aacccgatac cgcgccaaat
     1321 tttttcggtc tcggtaatgt aggcaactaa tttatcggta atctcttcga ttaccggttt
     1381 agaaaggtac tcataggtgc tttcgagatc cttaatgtcg gtgatgatga agtttgtggc
     1441 gtacttcttg ccaatttgca cgatagcatc tccatagatc atgtgctcaa gcggttcttc
     1501 caagtaaatg gtggggatac cggtgcaaac actgatctcc tctatagtga gcggcgcatc
     1561 atagcacgcc ttagcgatag ctttgtgcac ttgggtatta acgtatcttg tcggggcaaa
     1621 agagccattg cacatgacgg acatcttgat cttttcgtag tttcgttcca tttctgaaag
     1681 cctctctttc actttctccc tgccaacgtg aaggcgccat ttcacagttt cagcagagat
     1741 gcctagtttg catgagattt cgcggacgct cagattgtga acgtagtatt caactaggat
     1801 ctgtcggtac gtagagctta gagtgtgtag cgcttgaaaa acggcctgga actcatcgct
     1861 ggactcgtca aaagagctgt cgaagtcttc cgcaaaatca gaaacatact ctgcgccatg
     1921 aaccagaaca aggtctttgt tttcggtatt gacctttcta ccatagcggt tgtgagctac
     1981 tttccaaaca taagcatcta agttggcgat cttcgattgg ttaatgccct ggagaacgca
     2041 gagaagtatc tcctgagcta ggtcttgtgc atctgcaaaa gaagataacc gctttgcgca
     2101 gaagcgatag atcggctcta caaaccgcag tagttcgttg ttcatctgtc cgctcctttc
     2161 ctcagcaact tgaaacatgt ttcgcctata tagtgcagac tttcagagtt tggataggtg
     2221 ttcggtggat aatctttagt atattttacc tgagaagacc gatggattag aagttaggtt
     2281 caggacaacc tcaagcgaaa tccaaaaact tgttgaattt gctgcagaag tgactgcagt
     2341 tgtggttctg cagagttgag gaaggactat ctctaagagc tactgaacta caaaatagac
     2401 acagctccac acgccacaaa caggaacttc aacatatagt ggcgtatgtt tccctacggc
     2461 aaccaattgt taactttcgt tgctcatcaa gacagccctt ttaagctatt ctggctatag
     2521 gaagggggca gaaatagatg tttggtgaaa acctcaaaag actacgcaaa caaaaaggac
     2581 tttctcaaga agaactagca gaacgactac atgttgtaag gcaaactatc tccaaatggg
     2641 agcaaggccg gtcggtgccc gatgcagatc tcctaatcag gatagcggag atctttgaag
     2701 tacccgtaag tacgctgctc ggagatgctg ttgaagtatc taacggaaca gacctggttg
     2761 cagagaaact ggagcaactg aacctgattc tggctgagag aaacagaaga agcaggatga
     2821 tctggaaagt agttggggta accttgattt ccattgcagt ccttactgtc ttaggcatca
     2881 taacagcctt gatagggctt gtagcataca caactgtcca gtctaccacc gctgaaaaga
     2941 cggaggtctt agttgagcct gtggaagtgg agcatcccgt tccatatgaa ctcgaagctc
     3001 tcgaggaaga ccttgtgttt atcgagtaga agcatgatag gcaaatatcc attgcacaaa
     3061 aaaagctatg gctc
//
LOCUS       DAOSDA010000207         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_20481_length_3071_cov_3.084881, whole genome shotgun sequence.
ACCESSION   DAOSDA010000207 DAOSDA010000000
VERSION     DAOSDA010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20481_length_3071_cov_3.084881"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..803)
                     /locus_tag="PLW82_05485"
     CDS             complement(147..803)
                     /locus_tag="PLW82_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOJ46760.1"
                     /translation="MELFSLQDWQYMLLTLPILVMALSFHEYAHAYTANKLGDPTPKK
                     QGRLTTNPLKHINLFGFLSFLLLGFGWARPVRTDSTYFEKPRRDLALVAVSGPLTNLF
                     LAALFAVAIFIFFRMINPMLLQTSIAAFLVGEFLWSGFYLNLVFFFLNMLPIPPLDGS
                     RLLYSLLPKGSDGAIIILERYGIFVLLFLIVSGTLGKILGPVVNSIVVKYANLLVVLL
                     "
     gene            908..1966
                     /locus_tag="PLW82_05490"
     CDS             908..1966
                     /locus_tag="PLW82_05490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ46761.1"
                     /translation="MINVTDYTLATRIELVQDDQPISWLTLNHLNIRVWGKTFTCGGI
                     GGVGTKEEFRRRGYSRSVLDKSVEVMKDKGFHFSLLFGIPNFYYRWGFASCLPQNRVE
                     IPTYQAERAPLTHEVEEMRPEDFHAIVEIFNDNNKCRTGSVVRDPEHFTKFRLGSHFG
                     VRAQGYVLKKDGQIEGYLVLDQLDRKINAAEIEGRNISAYYSAMAFLAKLAVEKRTEQ
                     ISANLPADHQFTLIARNYGATVAANYIYNADGMGRIITQEPVLKALEEDLYRNCGLDS
                     ETVSLVFSTDLGTTKLGAGKEAKVEIDQMALTQLIFGYRDPKSLIHEGRLKSDLSPET
                     LTAMFPLRIAHLSGPDKF"
     gene            complement(2118..>3071)
                     /locus_tag="PLW82_05495"
     CDS             complement(2118..>3071)
                     /locus_tag="PLW82_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46762.1"
                     /translation="VRNFNVNGVPVLCGHRQQTLFLLSQDKFIKSITLVSTNALPNGM
                     YSLTYTAENVAASPVFIISLYSETGKVSEITYEGHSLDPTTPLHLSDLEVVFNPLTPL
                     NLLKADIGTINDAKLLFVDVKSMIYPGKYKILVQKSSSGLEAYLGRFSATIKNGKAEV
                     GGLALEFSRHLHVAEPNSRISEVSVGYVTRNVTVQIETGILPLTLEFEVAGKRQALEL
                     MPMLPESLHGNAFGKIVTLADLSLGTGFDPSVNLEVLEAINLRLNQYDKILSDRLTYL
                     KKLFSSQYASERKLPHKDPTVLLKAIEDKLKFIPFSVFLKE"
BASE COUNT          838 a          692 c          804 g          737 t
ORIGIN      
        1 aggacattgc cctttcacgg caaaggcacg ggttcaaatc ccgttgaggg taccagatat
       61 taacattttt ggtgtggttt tgcgcacctt ttttttgccc aaaaataaag gaagaaggta
      121 ctcggtgacg gagtaccttc ttccttttat agcaagacaa ccagtaaatt ggcgtatttg
      181 accacgatcg aatttacgac aggcccgagg atcttaccta aggttcccga gactatcaga
      241 aacagcagaa caaaaatgcc gtaacgctcg agaattatga tggctccgtc agagccttta
      301 ggcagcaggc tataaaggag tctcgaaccg tcaagcggag ggatcggcag catattcaga
      361 aaaaagaaaa ccaggttgag gtaaaacccg gaccaaagaa actctccgac aagaaatgca
      421 gcaatgctag tctgcagcag catgggattt atcatccgga agaagataaa aatcgcaaca
      481 gcaaacaggg ctgccaggaa caggttggtg agggggccgg agacggccac tagagccaga
      541 tcccgtctgg gtttttcgaa ataggtcgaa tcggttctta caggtctagc ccaaccgaaa
      601 ccgagaagca ggaaggaaag gaaaccaaag agattgatat gtttcagcgg gtttgtggta
      661 agccttccct gtttcttcgg tgtgggatct ccaagcttgt ttgctgtgta ggcgtgcgcg
      721 tactcgtgaa acgacaaggc cataaccaaa atcggcagag tgagcaacat atactgccaa
      781 tcctggagcg agaatagttc cattgatagt cttcctttac cattagttta ctttttaatt
      841 agactttaag aggtaaatac ctttttaaag cgaaaaaaga gttagtttca agttgggggg
      901 agtattcttg attaacgtaa cagactacac actggcaaca cgtatcgaac tggtccaaga
      961 cgaccagccc atcagctggc tcacattaaa ccatctgaac attcgcgtgt ggggcaagac
     1021 gttcacctgt ggaggaattg gaggcgtagg gaccaaggag gagttccgca ggaggggcta
     1081 ctcgcgaagc gttttagata agagcgttga agtaatgaag gacaaagggt tccacttctc
     1141 gctacttttc ggcataccaa acttctacta caggtgggga tttgccagct gtctgccgca
     1201 aaaccgcgtg gaaattccta cgtaccaggc agaaagagcg ccgcttaccc atgaggtgga
     1261 agaaatgcgc ccagaggatt tccatgcaat cgtggagatc ttcaacgaca acaacaaatg
     1321 tagaaccgga tctgtggtgc gagatcccga acacttcacc aagttcaggc tgggcagcca
     1381 ctttggcgtc cgggctcagg gctacgttct gaaaaaagac ggccagattg agggttactt
     1441 ggtgttggat cagctagatc gtaagatcaa cgcggctgaa atcgaagggc ggaacatttc
     1501 tgcttactac tcggcaatgg cgtttcttgc caaactagct gtggaaaaac gcactgagca
     1561 gatttcagct aacctgcctg ctgatcacca gttcactttg atcgcccgta attacggcgc
     1621 aactgtcgcc gctaactata tctacaacgc agacggcatg ggtaggatca tcactcaaga
     1681 gccggttcta aaggctcttg aagaagacct ttatagaaac tgcggattgg actctgaaac
     1741 cgtgagtctc gtcttcagta ccgacctcgg caccaccaag ctcggagcag ggaaagaagc
     1801 aaaagtcgaa atcgatcaga tggccttgac ccagctgatt ttcggctaca gagatccgaa
     1861 gagtttgatc catgaaggaa gattgaagtc cgatctctcg ccggagacgc tcactgccat
     1921 gttcccgctc cgtatagcgc atctcagcgg ccctgacaag ttctaagaag ggatgcgaga
     1981 ggttttgtgg tccattgatc cattcggtga taaagggagc cagataggcg ctccaggcgc
     2041 ctaggaagcc gttataatgg tgcatttgag catacggtac ccgtaagaaa aacgctaggt
     2101 tttactgcct agcgccttta ctctttaagg aacacgctga acggaatgaa cttcagttta
     2161 tcttcgatgg ctttcaatag caccgttgga tctttgtgag gtaacttcct ttcgcttgcg
     2221 tactggctcg aaaagagttt cttcaggtaa gtcagccggt cggacagaat cttatcgtac
     2281 tggttcaatc tgaggttaat cgcttccaaa acctcgaggt taacagaagg atcgaatccg
     2341 gttcccagac tcaagtcggc caaggtgacg atcttgccga atgcgttgcc gtggagagac
     2401 tcagggagca ttggcatcag ttcgagggct tgacgcttcc ctgcaacctc aaattcgaga
     2461 gttagcggca gaattccagt ttcaatctgc acagtcacat ttctggttac gtatcccaca
     2521 gagacctctg agattctgga gttgggttcg gcgacgtgca gatgtctgct gaactctaag
     2581 gccagaccgc caacttcggc tttgccgttt ttgatagttg cagaaaaacg ccccagatat
     2641 gcttccaatc cgctgctgga tttttgaact aggattttgt acttgccagg atagatcatg
     2701 gatttaacat caacaaaaag gagcttagca tcattgatag taccgatatc tgctttaagc
     2761 aggttgaggg gagtaagtgg gttgaaaaca acttctaaat ccgaaaggtg caaaggtgtt
     2821 gtaggatcaa ggctgtgtcc ctcgtaggtg atctcgctga ctttgccggt ttcgctgtac
     2881 aacgaaataa tgaagacagg tgatgcggcg acgttttcgg cagtataggt cagactgtac
     2941 atgccgtttg gcaacgcgtt tgtggagacc agggtgatgc tcttgatgaa cttatcctgg
     3001 gacagcaaga aaagggtttg ttgtcggtgc ccgcagagaa cgggaacacc gttgacattg
     3061 aagttccgga c
//
LOCUS       DAOSDA010000208         3063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_20557_length_3063_cov_3.873338, whole genome shotgun sequence.
ACCESSION   DAOSDA010000208 DAOSDA010000000
VERSION     DAOSDA010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20557_length_3063_cov_3.873338"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PLW82_05500"
     CDS             <1..887
                     /locus_tag="PLW82_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOJ46763.1"
                     /translation="LDPHFLIIEITESILMRKMDKAVLEIRKLQSHGIEVALDDFGTG
                     FSSLMYIKSMNIDILKIAGSFLEDVPENETNATIVKQTVDLARSLNLKTVIEGIETHD
                     QLAFLRRCDCATGQGYIYSRPLPAEEFEEYLEKRSIQPAVVEESAASYGVNRRKYFRV
                     SFNRLLEGRLTIAELYGRSVNVGNTKVLIKNIGPGGLCFLSTIRFPVDRHIVLRFTFE
                     LADENVEILGKPVWTRPLEDNLYEYGIEFAIDEIERQALTFILNQVQIRLKKDVLFDD
                     PHFVSTSPSDYFKALAAR"
     gene            complement(1036..1839)
                     /locus_tag="PLW82_05505"
     CDS             complement(1036..1839)
                     /locus_tag="PLW82_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOJ46764.1"
                     /translation="MNTPDVLKKKKLFLLDMDGTIYIENTLFDFTLDFLDYIKSIGGK
                     YIFITNNSSKSVTDYVDKLRRMGIDVDLSNFLTSSQATAVYLNRQFPGKKIYVMGTES
                     LKEEFRKAGIRITDKYEEDIDCFVAGFDTELVYQKLHDASVLLTEGVSFVATNPDLVC
                     PTSFGFIPDCGSMCMMLENATGRKPYYVGKPNPMMIEMAIEKSGFTKEETLVIGDRIY
                     TDVKSGINAGVTTALVLSGETKRADLEASTDKPDFVFEDVGELLELLKS"
     gene            complement(1943..2959)
                     /gene="galE"
                     /locus_tag="PLW82_05510"
     CDS             complement(1943..2959)
                     /gene="galE"
                     /locus_tag="PLW82_05510"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOJ46765.1"
                     /translation="MAILVTGGTGYIGSHTVVELLDSGYEVVIVDNFYNSKPEVLNRI
                     QDITGKDFKFYEIDIRDQEALDKVFSENQIEAVIHFAGLKAVGESVEIPITYYHNNIT
                     GTLMLCEVMQKHGVKKIVFSSSATVYGMDNKPPYTEDMPTSATNPYGRTKLMIEEILK
                     DIYVSDNSWGISILRYFNPIGAHASGKIGEDPNGIPNNLLPYITKVACGKREKLYVFG
                     DDYDTNDGTGVRDYIHVVDLAQAHIKALEKILSNTGVEIYNLGTGVGYSVLDVINTFA
                     RVNQVEVPYVITDRRPGDVATSFADPSKAFKELGWKAAKNLEDMCRDSWNWQKNNPDG
                     YSSE"
BASE COUNT          778 a          737 c          697 g          851 t
ORIGIN      
        1 agcttgatcc tcacttcctc atcattgaga tcactgaaag tatcttaatg agaaagatgg
       61 acaaagcagt tttagagatc agaaagctcc agtcccacgg cattgaagtt gctctcgacg
      121 atttcggaac tggattttct tcgttgatgt acatcaagtc catgaacatt gacattctaa
      181 aaatcgcagg ttctttcctg gaagatgttc ccgaaaacga gaccaacgct acgatcgtga
      241 agcaaactgt agacctggcc agaagcctta acctgaaaac ggtcattgag ggcattgaaa
      301 cacacgacca gttggccttc ctcagaaggt gcgactgtgc tacagggcag ggctatatct
      361 acagcagacc tctgcctgca gaagagtttg aagagtacct tgaaaaaaga agcattcaac
      421 ctgcagttgt tgaagaatcg gcggcaagct acggggtaaa cagacgcaaa tacttcagag
      481 ttagtttcaa tagactcctt gaaggtaggc ttacgatcgc agagctgtac ggcaggtctg
      541 tgaatgtagg aaacaccaaa gttctcatca agaacatcgg tcctggtggt ctctgcttcc
      601 tctccaccat tagattccct gttgaccgac atatagttct gcggtttacc tttgaactgg
      661 cagacgaaaa tgtcgagatc ctagggaaac ccgtttggac ccgaccttta gaggacaacc
      721 tctacgaata cggaatcgag tttgccatcg acgagattga aagacaagcc ctgacgttca
      781 tattgaacca agtgcagatc agactgaaaa aggacgtcct tttcgacgac cctcattttg
      841 tatctacctc acccagcgac tattttaaag ctctagctgc aagataaccg gactagtggc
      901 aaaaagccat ctacgcgttt cccaaaccca aatacagaaa cacccctttg cctgaggcaa
      961 gggggtgttt tcttcgctta agcgttagat atgctttgtt aacatttatc ggggtcatct
     1021 atacacaacc aagggtcagc ttttgagaag ctcaagcagc tcacccacat cttcaaacac
     1081 gaagtctggt ttgtcggtgg aggcttcgag atcggcacgc ttggtctcgc cgcttagaac
     1141 tagagccgtg gttacacctg cgttgatccc gcttttaacg tcggtataga tgcggtcgcc
     1201 aataaccaga gtctcttctt tggtgaaacc gcttttttct atagccatct cgatcatcat
     1261 gggattgggc ttccctacgt agtacggttt cctcccagtg gcgttttcca acatcatgca
     1321 catagagccg cagtcaggaa tgaagccgaa ggaagtggga cagacgagat cggggtttgt
     1381 agcaacaaag cttacacctt ctgtaaggag tacgctagca tcgtggagct tttggtagac
     1441 cagctccgta tcaaaccctg caacaaagca gtcgatgtcc tcttcgtatt tgtcggtaat
     1501 cctgatgccc gccttcctga actcttcctt taaagactcg gtacccatga cgtagatctt
     1561 tttgccgggg aactgccggt ttaagtagac cgccgtagct tgagatgatg taagaaagtt
     1621 tgagagatcc acatcgatac ccatcctgcg gagtttatct acataatcgg ttacgctctt
     1681 ggaggagtta ttggtaataa aaatatactt gccgccgatg gacttgatgt aatccagaaa
     1741 atccaacgta aaatcaaaca gtgtgttttc tatatagatt gttccgtcca tatccaagag
     1801 aaagagtttt ttctttttca agacgtctgg tgtgttcatt gaagcagtac ctccaactgt
     1861 tttccttgcc ggcatctctt tccaacactg tgagaaacac agcgcagcac gatctactta
     1921 taccacatat cccaaattat agctactccg acgagtagcc gtcggggttg tttttctgcc
     1981 agttccaaga atccctgcac atatcttcga ggttcttggc tgccttccag ccgagctcct
     2041 tgaatgcctt gctgggatct gcgaaagatg tagcaacgtc gccaggtctt ctgtctgtga
     2101 ttacgtaagg cacctctacc tgatttaccc gagcaaaggt gttgatcaca tcgagtacgc
     2161 tgtagccaac accggtacct aggttgtaga tctcaactcc agtgttactt aatatcttct
     2221 ccagagcttt aatatgagcc tgggctagat ccacaacgtg gatgtagtcc cttacccctg
     2281 ttccatcgtt ggtgtcatag tcgtcgccga aaacgtagag cttctctctc ttgccgcaag
     2341 ctaccttggt gatgtagggt aggaggttgt tggggatgcc gtttggatct tcgccgattt
     2401 taccgcttgc atgagctccg atagggttga agtatctgag gatggagatc ccccaagagt
     2461 tgtcggagac gtaaatgtct ttgagaatct cttcgatcat cagcttggtt ctgccgtagg
     2521 ggttcgttgc gcttgttggc atatcttctg tatatggtgg tttgttgtcc atgccgtaga
     2581 ccgtcgcaga agagctgaag actatcttct tcacgccgtg cttctgcata acctcacaga
     2641 gcatcaaggt gccggtgatg ttgttgtggt agtaggtgat gggaatctct accgattcac
     2701 caactgcctt cagccccgca aagtgaatca ccgcttcgat ctgattctca ctgaagactt
     2761 tgtccaaagc ctcctgatct cgaatgtcta tttcatagaa cttaaagtct ttccccgtga
     2821 tgtcttggat acgattcaaa acttcaggct tactgttgta gaagttgtct acgattacca
     2881 cttcatagcc ggagtccagc agttcaacta cagtatggct gcctatgtat cccgtaccgc
     2941 ctgtgacaag tatggccatt tgaatttcct cccaaatcta tgatggttta ttctttatac
     3001 agtaaaggag ctaaatccta caatacctgc agatttagct cctgttgcat gcaattatca
     3061 tag
//
LOCUS       DAOSDA010000209         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_20606_length_3058_cov_2.869131, whole genome shotgun sequence.
ACCESSION   DAOSDA010000209 DAOSDA010000000
VERSION     DAOSDA010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20606_length_3058_cov_2.869131"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLW82_05515"
     CDS             complement(<1..373)
                     /locus_tag="PLW82_05515"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ46766.1"
                     /translation="MRSKYKLVITDLDGTLLDYKQRIHPANVKALQELKDAGIPVVPA
                     TGRGKRSAEGVINGLPLSEYLVCFQGAAVFKGDRAIWHQPVGDVLPYIKLLREHKNVN
                     SAVFATHAEGVEDLTMFAPYYA"
     gene            599..826
                     /locus_tag="PLW82_05520"
     CDS             599..826
                     /locus_tag="PLW82_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HOJ46767.1"
                     /translation="MDLKNIFTSEQRKALVEEIKEYFLNEHDIELGDLACTLLLDFFS
                     EKIGPEFYNQGVFDSYKYLQNSLDDLLGIQE"
     gene            complement(1000..2004)
                     /locus_tag="PLW82_05525"
     CDS             complement(1000..2004)
                     /locus_tag="PLW82_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46768.1"
                     /translation="MGFLMSVNVVKEGKDDNNINKIEECVVKALDDKYERIDSADDSD
                     RTILIVPNNRFVSIFDDFAPYMYEDQFSIFPRRLSRTLDAPIVSLYVEDGDIIQLRLF
                     QEGQLLDEYCNMPWFVDETVPTGTVTGDAEIWVKQLKLDATPEELESAWSNLDLLGEQ
                     VVERLWEIFGGDSSLFMRDKIFFLEDLEQYSLMHFKRKPDVQLGNLEEYMVKLYEQDK
                     LIRKKQRLFFKLVEEVFQPVFAQHGFTRTEKFKHYRIYEKSYSNDSSVILFVQFYELH
                     FSLNIYCKKEECNGLHLINYLYTLEDGEAELLEAIHRARDAFLAEGLQFVQRVEGMQV
                     "
     gene            complement(2350..>3058)
                     /locus_tag="PLW82_05530"
     CDS             complement(2350..>3058)
                     /locus_tag="PLW82_05530"
                     /inference="COORDINATES: protein motif:HMM:NF027473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4855 domain-containing protein"
                     /protein_id="HOJ46769.1"
                     /translation="LLQDKEHKVKVILTLVDPSPLQNRFGALEPGGKNLNFNHEQVGV
                     KEAFQNRLKAVEWLINQFLDRFAKADAQYLELIGFYWQPESISYHHSPYEDEFVRKVA
                     DLVHEKGLKLFWIPFYGSPGSYDWHNYGFDAAILQPNYVFSDSKEDRVKTAASIAYHL
                     GMGVEMEKHWNDTLIERNKWRDYLNGGVKYGYMNGLVAYYQNIKDFGRAAATFDKRTL
                     YYDYVYQFVKGEYEITE"
BASE COUNT          754 a          769 c          652 g          883 t
ORIGIN      
        1 ccgcgtagta gggagcgaac atggtcaggt cctctacccc ttctgcatgg gttgcaaaga
       61 ctgccgagtt cacgttcttg tgctctctaa gaagcttaat ataaggcagg acgtcgccta
      121 caggttgatg ccagatagct ctgtctccct tgaaaactgc agcaccctga aaacaaacaa
      181 ggtattcact taagggtagc ccgttaataa cgccttctgc agatctctta ccccttcctg
      241 ttgccggaac cacaggaatt cccgcgtctt tcagttcttg aagggcttta acatttgccg
      301 gatggattct ttgtttatag tccagtaatg taccgtctag gtctgtgatc accagcttat
      361 acttactacg caaaaggttt gcctcctagt cttctatgtg aaaatcgaca tgttggtgta
      421 tacttatgtt tagtcaaaaa aatgtcagcg ttgtttaaat tcttccgacg cctgccctag
      481 acctctttta tttgcaaaac tagaaacctc agcctgtgtg tccctccgcc tttgagtgca
      541 gccccaagcg taagcacagt ttttgggatt taaacagaac agcaaaggag ttttagccgt
      601 ggatctgaaa aacatcttca ccagcgagca gagaaaagcc ctggtcgaag agatcaagga
      661 gtactttctt aatgagcacg acattgagct tggtgatctt gcatgtactc ttctcctaga
      721 cttcttttcc gagaagatcg gtcccgagtt ctacaaccaa ggtgtattcg atagctacaa
      781 gtatctccag aactccctag atgaccttct tggaatccag gagtaaacag attggaaatt
      841 caccgagcct ggttgggcag tatgcttaag aacctctttg cttgtgatcg gaacaagcaa
      901 agaggttctt aacttctaaa gtatttagtt aagtgcatgt atccgcaaat accatttacg
      961 tttatgcggc tagaccttga aaccgtaaga ggctctgcgt cacacctgca ttccttcaac
     1021 ccgttgtaca aactgaagtc cttcggccaa aaatgcgtcc cttgctctat gtatcgcttc
     1081 tagaagctct gcttccccgt cttcaagagt gtataagtaa ttgattaagt gcaggccgtt
     1141 gcactcttct tttttgcagt agatgttgag agaaaagtgc agctcgtaga attgcacaaa
     1201 caggatcacg cttgagtcgt tgctgtagct tttttcatag atgcggtagt gtttgaactt
     1261 ctcggtgcgc gtaaaaccat gctgtgcaaa caccggctga aagacctctt caaccagttt
     1321 aaaaaacagc cgctgttttt tgcgaatcag cttatcctgc tcatacagct taaccatgta
     1381 ttcttccaag ttgcccagct gtacatctgg ttttctcttg aagtgcatga gggagtactg
     1441 ttccaaatct tctaagaaaa agatcttgtc tctcatgaac aaacttgagt ctccgccgaa
     1501 gatctcccac agcctctcta ccacctgctc gcctaaaaga tccaggttgc tccaggcaga
     1561 ttccagctcc tccggcgtgg catcaagctt caattgctta acccagatct ctgcatctcc
     1621 tgtaacagtg cctgtgggca ccgtctcgtc aacgaaccac ggcatattac agtactcatc
     1681 gaggagctgt ccctcttgaa acagcctcag ctgaatgata tcgccatctt caacgtacag
     1741 actgacaata ggggcgtcta aggttcttga gagcctgcgg ggaaagatgg aaaactggtc
     1801 ttcatacata tacggggcaa aatcatcaaa aatgctgaca aacctgttgt ttggcacgat
     1861 aagtattgtg cggtccgaat cgtcagcgga atcgattcgc tcatacttgt cgtcaagagc
     1921 ttttaccaca cactcttcaa tcttatttat gttattgtcg tctttgccct ctttaaccac
     1981 atttacactc atcaggaacc ccatcgatat tcctccctgg cttaaggcaa cagatttccc
     2041 gagaccgaaa gtttaagata ctccaaaagc accgcgtact ttgtttgtac tctttcagtt
     2101 tagccaaata caattacgct aggttacatg ctcccgtctc cgtttctgct tgcaaggggc
     2161 aagattacac atgctttcca tttttccctc tagtactata tttgccttat ataactgcat
     2221 tcctctattt tgtttctgtc agttcgaatt tcgcgaatca aataaaacaa aatgcgattt
     2281 cggtctcgct ccccctagcc ctacatgaag aaaggctgcc gtgggatccc acagcagcct
     2341 gcgttacact tattcggtta tctcatactc accctttaca aactgataga cgtaatcgta
     2401 gtagagggtg cgcttatcaa aggttgcggc agctctgcca aaatctttga tgttctggta
     2461 gtatgcaacg agcccattca tgtatccgta cttcacgccg ccgtttagat agtctctcca
     2521 cttgtttctt tcaatgaggg tgtcgttcca gtgcttctcc atctccacgc ccatgcccaa
     2581 gtggtaggcg atgctggctg cagttttgac tctgtcttcc ttgctatctg agaacacgta
     2641 gttcggctgg aggatagctg catcgaagcc gtagttgtgc cagtcatagc ttcccggaga
     2701 accgtagaag ggtatccaga aaagctttag gcctttttcg tggacaagat ctgcaacctt
     2761 cctgacaaac tcgtcctcat aggggctgtg gtggtaggag atactttcag gttgccagta
     2821 gaagccgatc agttctaagt actgggcatc tgccttagca aagcgatcca agaactgatt
     2881 gatcagccac tccactgcct tcaagcggtt ttggaaggcc tcttttaccc ctacttgttc
     2941 atggttgaag tttaggttct ttcctcccgg ctccagggct ccaaacctgt tctggagggg
     3001 tgaggggtcc actaaggtca ggataacctt caccttgtgc tccttatcct gcagcagg
//
LOCUS       DAOSDA010000210         3045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_20732_length_3045_cov_2.325084, whole genome shotgun sequence.
ACCESSION   DAOSDA010000210 DAOSDA010000000
VERSION     DAOSDA010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20732_length_3045_cov_2.325084"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLW82_05535"
     CDS             complement(<1..544)
                     /locus_tag="PLW82_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HOJ46770.1"
                     /translation="MEKTITELRVERLASGLKDGEFLLVTDLKNVRYLSGYTGTSGIV
                     LLSSAKAYFLTDFRYVEQAKIQCPHLELVVHKPIVWDSVADLLGEAEVLYVEESNLTL
                     ASYNQLKSRIKAEIKEAKGPVEKLRMVKSPEEIELISIAARITDEAFMEICGMIKPGV
                     TEMEIARELEFSMKRKGASHT"
     gene            complement(547..993)
                     /gene="aroQ"
                     /locus_tag="PLW82_05540"
     CDS             complement(547..993)
                     /gene="aroQ"
                     /locus_tag="PLW82_05540"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOJ46771.1"
                     /translation="MKGATIWVINGPNLNLLGEREPEIYGRETLTDVEKKIGEIAEEN
                     QVNLEFFQENEEGKLVTLIQKARLHADGLLLNPGAYSHYSLAIYDALRAITIPKVEVH
                     ISQVAAREEFRRNLLTAGACDGLIAGLGTYGYVLGLYALLELIKEG"
     gene            complement(999..2126)
                     /gene="aroB"
                     /locus_tag="PLW82_05545"
     CDS             complement(999..2126)
                     /gene="aroB"
                     /locus_tag="PLW82_05545"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOJ46772.1"
                     /translation="MDVALNLPNHSYTIYIEPGILTSVGRKIKELDISLGLAISKVLI
                     VADSNTAPLYLEEAVRSFQEVGYEAFQMVLPAGEIFKTAASVELIWEKALDSGLDRKS
                     LMVALGGGVVSDLTGFAAATYLRGLYYASIPTTLLSQVDASVGGKTGFNLKSGKNLVG
                     AFYQPIGVFADPNTLLSLSQREYRNGYAEVIKHALLSRSDLLVFLEGNLEKIESRDPW
                     FLAKLVEKNVRIKADVVASDEKEAGRRAILNLGHTLGHALEELGGYKDYSHGEAVAIG
                     MTAACAISLRRGLITEQQFARVKSLIEKVGLPTKPSSPVEPWVWWEKTYKDKKAASGV
                     VYWVLPQKNQEGNLEFPFDHALFGVRVEYEEFQSVLDLLGE"
     gene            complement(2111..2593)
                     /locus_tag="PLW82_05550"
     CDS             complement(2111..2593)
                     /locus_tag="PLW82_05550"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOJ46773.1"
                     /translation="MLVYLVGFMAAGKTTVGRKLAKMLGVPFIDLDAVIEEAAQMTIP
                     EIFANEGEAGFRKRERLALLAKPDKGVIATGGGIVTVPENRCFLKKQPVVYLQADLSV
                     LRKRIAQTPGTRPLADHKLEERFLQREPLYREVSDLVVDTSGKTADEVAREVYQWMLR
                     "
     gene            complement(2593..>3045)
                     /locus_tag="PLW82_05555"
     CDS             complement(2593..>3045)
                     /locus_tag="PLW82_05555"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOJ46774.1"
                     /translation="RRLDGRIAQAVMSIPAIKGVEIGLGFAAAGLPGSMVHDAIGYSD
                     RYFRLSNNAGGIEGGISNGEDLVVRAAKKPIPTLYKPLKTVEIKTHEKMEASIERSDV
                     TAVPAASIVVEAMVAWVIAESLVEKFGGDSLTDMKKSVAAYLERLGEI"
BASE COUNT          695 a          875 c          654 g          821 t
ORIGIN      
        1 aggtgtggct tgcacctttt cgcttcatgg aaaactccag ttctctggcg atttccattt
       61 cggtaactcc tggtttaatc ataccacata tttccatgaa agcctcatcg gtaatccgcg
      121 cagcaatgct gatcagttcg atctcctctg gagacttaac catgcggagc ttttccaccg
      181 ggcctttagc ctctttgatc tccgctttga tccttgactt aagctgattg tagcttgcca
      241 aggtcaggtt gctttcctcc acatacaaaa cctctgcttc tcccaagagg tcggcaactg
      301 aatcccacac gatcggtttg tgaaccacaa gctcaaggtg ggggcactgg atctttgcct
      361 gctccacgta gcgaaaatcc gtaaggaagt aggctttcgc ggaactcaac agaacaatcc
      421 ccgatgttcc cgtatagccg ctcaaatagc gtacgttctt gaggtccgta acaagaagga
      481 actctccgtc tttcagtcca gatgcgagtc tttctaccct aagttctgta attgtcttct
      541 ccattttcag ccttccttta tcaactcaag tagagcgtat agtccaagta cgtaaccgta
      601 tgtccccagt cccgcaatga gcccgtcgca cgcaccggct gtaaggaggt tcctgcgaaa
      661 ctcctccctg gctgcaacct gcgagatatg aacttccact tttgggatag taatggcccg
      721 cagagcatcg tagattgcca gactgtagtg agagtaggct cccggattga ggagaagtcc
      781 atctgcgtgg agccgtgcct tctgaattag tgtaaccagt ttaccttctt cgttttcctg
      841 gaaaaactcc agattaactt ggttttcctc ggctatttca cctattttct tttcaacatc
      901 cgttaaagtt tccctgccat atatttccgg ttctctttcg ccaaggagat tgaggtttgg
      961 cccgttgata acccaaatcg ttgcaccttt cacctgcatc actccccaag aagatctaag
     1021 acgctctgaa actcttcata ctctactctt accccaaata gtgcgtgatc aaagggaaac
     1081 tccaggtttc cctcctgatt tttttgggga agcacccagt atactacgcc agatgcggct
     1141 ttcttgtctt tataagtttt ctcccaccac acccacggct ccaccggaga gctgggcttg
     1201 gtgggaagtc cgactttctc tataaggctt tttaccctag caaactgctg ttctgtaatc
     1261 agccctctcc tcaggctgat ggcgcatgct gccgtcatac cgatggccac agcttcaccg
     1321 tgggaataat ctttgtagcc accgagctcc tctaaggcat gccctaaggt gtgtcccaag
     1381 ttcaaaatgg cccttcttcc agcttccttc tcatcggagg ctaccacatc ggctttgatc
     1441 ctgacattct tttccacgag ctttgccaaa aaccaaggat ccctggactc gatcttttcc
     1501 aggttccctt cgagaaacac caaaagatcg cttctggaaa ggagcgcatg cttgataact
     1561 tcggcgtagc cgttgcggta ctccctttga gacaacgaca gcagagtatt aggatcggca
     1621 aacaccccga tcggttggta gaatgcgcct acgaggtttt ttccagactt gaggttgaag
     1681 ccggtctttc cccctacaga agcatctacc tgagaaagaa gcgttgtagg aatgctggca
     1741 tagtagaggc ctctaaggta cgttgcagcg gcaaaaccgg ttaggtcgct cactacgcct
     1801 cctccaagag ctaccatcaa actctttcga tccaacccgc tatccaaagc cttctcccag
     1861 atcagctcca cacttgcagc ggtcttaaag atctctcctg caggcaggac catctgaaaa
     1921 gcctcatagc ccacctcctg aaaggacctt accgcctctt ccagatagag tggtgcggtg
     1981 ttggagtctg ctacaatcaa caccttcgag attgcaaggc ctaaagaaat gtctaattct
     2041 ttaatcttcc gtcctacaga cgttaaaatg cccggttcga tataaatcgt atagctgtga
     2101 ttcggcaagt tcaacgcaac atccactggt atacctccct tgcaacctca tctgcagttt
     2161 tgccactagt gtccaccaca agatcgctga cctctctgta cagaggttcc ctttggagaa
     2221 aacgctcctc cagtttatga tctgcaaggg gccttgtccc cggagtttgg gcgatgcgct
     2281 ttctcaacac cgaaagatct gcctgcagat agactacagg ctgcttcttt agaaaacagc
     2341 ggttctccgg cactgtgacg atacccccgc cggttgcaat aacaccttta tcgggcttcg
     2401 ccaaaagggc aaggcgttct cttttccgaa agcccgcttc cccttcgttt gcgaagatct
     2461 ccggaatggt catttgggcc gcctcttcga ttaccgcatc caggtcgatg aaaggtacac
     2521 ccagcatttt cgcaagtttc ctacccactg tggtctttcc tgcagccatg aaccctacta
     2581 gatacaccaa cattagatct cacctaggcg ctcaagatac gccgcaacgc ttttcttcat
     2641 atctgtcaaa gagtctccac caaacttctc cacaaggctc tctgcaatga cccaggcaac
     2701 catagcttcc acaactatgc tagctgcggg aactgcggta acgtcggatc tttcgatgga
     2761 agcttccatt ttctcgtggg tcttaatctc aactgtcttt agaggtttat acagcgttgg
     2821 tatgggcttt tttgcagctc tgaccaccag atcttctccg ttggagatcc cgccttcgat
     2881 tcctccggca ttgttggaca ggcggaagta cctatcgctg tagccgatcg catcgtggac
     2941 catgcttccg gggagacctg ccgcagcaaa gccgagcccg atttccacac ccttgatggc
     3001 gggaatgctc atcactgctt gggcaatcct tccatcaagc cttct
//
LOCUS       DAOSDA010000211         3031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_20852_length_3031_cov_2.257728, whole genome shotgun sequence.
ACCESSION   DAOSDA010000211 DAOSDA010000000
VERSION     DAOSDA010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20852_length_3031_cov_2.257728"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLW82_05560"
     CDS             complement(<1..730)
                     /locus_tag="PLW82_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009646662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOJ46775.1"
                     /translation="MRKTILVLILAITVTAFAAAVTLKIGATPRPHAEILEVIKPLAA
                     EKGINIEIVVFTDYVLPNLALADGELDANFFQHVPYLDTFKSDRRLDLASLVAVHVEP
                     MGLYSNKIKSLSELKNGALIGIPNDPTNGGRALLLLQTARLIEVDKAAGITPTVFDIT
                     KNPKNLKFTELEAAMLPRSLQDLDAAVINTNYALEGGLVPTKDALIMEGEESPYANIV
                     AVRTADLEKPEFAILAELLTSDAVR"
     gene            complement(745..1404)
                     /locus_tag="PLW82_05565"
     CDS             complement(745..1404)
                     /locus_tag="PLW82_05565"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HOJ46776.1"
                     /translation="MFNNPMIWNALKTALLETLYMVGVSTFFAVLFGLPLGILLTITS
                     PGHIKPNKLINTVLGAVVNLGRSIPFIILMVAIINFTRWLVGTSIGTTAAIVPLSISA
                     TPFFARVTEQSLREVAKGVIEAAEAMGATTLQIIFKVLLPEALSSLVLGVTITAVNLI
                     GYSAMAGAIGGGGLGDLAIRYGYQRFRPDIMIITVIVLVILVQGVQTVGSFIANRLDH
                     K"
     gene            complement(1397..2410)
                     /locus_tag="PLW82_05570"
     CDS             complement(1397..2410)
                     /locus_tag="PLW82_05570"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HOJ46777.1"
                     /translation="MIRFENVTKIYKGENEVVALKNIDLTINDGEIFGIIGLSGAGKS
                     TLVRCINLLETPDEGRVLVDGVDMMNLTRRELRQHRKSIGMIFQHFNLLSSRTVAENV
                     AFPLELIGVPKRQRIKKVRDLLELVGLSDKARAYPSQLSGGQKQRVGIARALANDQKI
                     LLCDEATSALDPQTTMSILELLKKINRERGITVVIITHEMHVIKEICDRVAVIEDGRI
                     IETGIVEDVLLNPTTETARTFLKTVIKSEVPPEVAARLKESNGGELLQVTFLGGSTGE
                     PIINEVVKNFDVVVNILYGNIDQIHDITFGMLVLKLTGPQRKQAKAFLEEKGLKVKVI
                     DDV"
     regulatory      complement(2474..2573)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT          736 a          731 c          704 g          860 t
ORIGIN      
        1 ctcttaccgc atcggaggtt agaagttccg cgagaatcgc aaactcaggt ttctccagat
       61 ctgcggttct tacagcaaca atgtttgcgt atggcgactc ttcaccttcc atgatcaggg
      121 catctttcgt tggcaccaaa ccgccttcta aggcatagtt ggtgttgatt accgctgcat
      181 ctagatcctg gagagacctc ggaagcattg cagcttcaag ctcggtaaac ttaaggtttt
      241 tcgggttctt ggtgatgtca aacacagtag gcgtgatccc cgcggctttg tctacctcga
      301 tcaatctggc agtttggagc agaagcaaag cacggcctcc gtttgtggga tcgttgggga
      361 tgccgatcaa cgcaccgttc ttgagctcgg acaggctttt aatcttgttg gaataaagac
      421 ccatgggctc tacatgcact gccacaagag acgcaaggtc caagcgcctg tcgctcttga
      481 acgtatccag gtagggaaca tgctggaaga agtttgcatc aagttctcca tctgctagcg
      541 ccagattggg cagaacatag tctgtaaata cgacgatttc gatgttgatt cctttttcag
      601 cagccaaagg tttgattact tctagaatct ctgcatgggg tctgggggtt gcaccgatct
      661 tcagtgtgac tgcagcggca aatgcggtaa cagtgattgc caatatcaag actagaatgg
      721 tttttctcat ttgatgtacc tcctttattt gtggtcaagg cggttggcaa tgaagctgcc
      781 aactgtctgg acgccctgaa ccaagataac caaaacgata acggtaatga tcatgatgtc
      841 aggcctaaag cgctgatacc cgtatctgat ggctagatcg ccaagtcccc caccaccgat
      901 tgctcctgcc atagctgaat aaccgatgag gtttacagcg gtgatggtta cacccaatac
      961 cagcgacgag agagcctcgg gcaggagtac tttgaagatg atctgcaggg tggttgcacc
     1021 catagcttca gcagcttcga tcaccccttt agctacttcc cttaaagact gctcggttac
     1081 tctggcaaaa aacggcgtag ctgagatgct cagtggtacg atggctgcgg tggtgccgat
     1141 ggaagtacct acaagccacc tagtgaagtt gatgatcgcc accattagaa tgatgaaggg
     1201 aatagaccgg cctagattga ctactgcacc taagaccgtg ttgatcaact tgtttggttt
     1261 gatgtggccg ggggacgtaa tcgtcagcag gatgccgaga ggtaggccga atagcactgc
     1321 aaaaaacgtc gacactccca ccatgtatag agtttccaga agtgccgtct tcagcgcatt
     1381 ccagatcatc ggattgttaa acatcatcta tcaccttcac cttcaggccc ttttcttcga
     1441 ggaaggcttt ggcctgtttc ctctgaggcc ccgtaagctt caacaccaac atgccaaagg
     1501 ttatgtcgtg aatctgatcg atattgccgt agaggatatt caccacaaca tcgaagttct
     1561 tgaccacttc gttgataatg ggctcaccag tactacctcc aaggaaggtg acctggagca
     1621 gctctccgcc gttactttct tttagccgtg ccgcaacttc tggcggcact tcgctcttga
     1681 tgactgtttt taagaaagtt ctggcggttt ctgttgtggg attgagaagg acgtcttcca
     1741 cgataccagt ttcgatgatc ctgccgtcct cgatcaccgc tactctgtcg cagatttcct
     1801 taatcacgtg catctcatgg gtaataatga caacggtgat gccgcgctct cggttgatct
     1861 ttttgaggag ctcgagaata ctcatagttg tctgtggatc tagagcagag gttgcttcat
     1921 cgcagagcag gattttttga tcattggcaa gagcccttgc aatgcctact ctctgcttct
     1981 ggcctcccga aagctgggag gggtaagctc tggccttatc gcttaagcca accagttcta
     2041 aaagatctct aaccttctta attctctgcc gtttaggaac cccgataagc tccaagggaa
     2101 aggccacgtt ctctgcaacg gttcgcgaag acaagaggtt gaagtgctga aagatcatgc
     2161 cgatactctt gcggtgctgg cggagctctc tccgggtaag gttcatcata tccacaccgt
     2221 ctacaagcac tcttccttca tctggtgttt cgaggagatt aatgcagcgt accaaagttg
     2281 attttccggc accggaaagt ccgataatgc caaagatctc tccatcatta atagtgaggt
     2341 caatgttttt taaagcaacg acttcgtttt cgcctttgta tattttcgtg acgttttcaa
     2401 acctgatcaa gaagatcacc cccattaaaa aagcctcttc gataagaaga ggcgaaaaac
     2461 gtcattttca cctttcttat ctctcagaca cttgtctgct ggaattagca cccttgcatt
     2521 acgctggttg ctgggcttca ttgggccagt ccctccgcca ctcttgataa gaaagctatt
     2581 tttattcatt tgtaggccag attctgtaag aatctatatt gattagtagt atagcattag
     2641 accgacaggt gggcaagagt tattttttgc tttttttcga tggcgctggt ggtaagtcca
     2701 gtctcaaact cctgcagttc tgctctaaga tacactacct gcttaacacc ttaaaaccga
     2761 ttctgtctat actcagtcag cggaagcaag gtaagctgca aaggatttca gttatgtaag
     2821 cttatgcgct actgttgcat tttactacaa ccaaacgaga aaaccaactg agtttttcgt
     2881 ctcagttggt tttcctatta ggtcctattc tattgaatga caattaaaat ttataaagcc
     2941 gtttttcttc ttgataactt gcggtaacct tagatgtgct ttggccccct tgtattggac
     3001 actagcttgc tctttctctc acgctttcaa t
//
LOCUS       DAOSDA010000212         3023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_20917_length_3023_cov_4.682278, whole genome shotgun sequence.
ACCESSION   DAOSDA010000212 DAOSDA010000000
VERSION     DAOSDA010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20917_length_3023_cov_4.682278"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1451)
                     /locus_tag="PLW82_05575"
     CDS             complement(102..1451)
                     /locus_tag="PLW82_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOJ46778.1"
                     /translation="MSFFDHKRLPASLFQIDKERIRKGWYTDAYFLNQIRILQQLAEE
                     GYRFSGRSDLEDVDCSDVDTGNIEVEMQIFTRRSPGSLIVGTDEALSILESCTGYFDK
                     DGKWVDTYDHLEVWAVEDGVWVPYEGDPSFVKPVMKIRGRYREIGHLETVILGVLSEP
                     TRIATNVLNVLEACNGKDVLFFPARYVHYKMQAIGGYAFSLGVQAYNYRHGRKESKIF
                     VSTDAQGEFWGGRGGGTISHAAICCFLGDTVETMLQFARVLPRSYSRIALVDFHNDVV
                     GETKAVLKAMFDKYLEYKKQGNEEEAEKYRLLAVRPDTGGNMRDKSISPLGAKELDLG
                     VNPRMVWKLREALDTSYLTWDLPAEDLEEARKYCQGVKITVTGGFDVNKIQLFESLKV
                     PVDIYGVGSSLLTNCAKCGTNNDFTADIVRVKVGGDWHPMAKAGRRACENPLLKRIK"
     gene            1669..2091
                     /locus_tag="PLW82_05580"
     CDS             1669..2091
                     /locus_tag="PLW82_05580"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HOJ46779.1"
                     /translation="MIKQIWWFLIILIIGGAAMGLSRLLPQRKMTDLEYIYIEDAESL
                     PSYAADDEELLRDLEDGPVLIISRGEKSTGGYHIELIASELEDDHLEIEVTTSDPKPG
                     SFSIMVITHPYVHVKTEGAMTYSVTVNGSVAVERQSLL"
     gene            2267..2962
                     /locus_tag="PLW82_05585"
     CDS             2267..2962
                     /locus_tag="PLW82_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46780.1"
                     /translation="MFKRAAALFLFLLLSFVVCAESLSGFPWNAGVDEVKAQEEIDPL
                     YETSSFIVYETTLFGRKVQKIYSFDENRLTSIIYIFYELPSELKGYLHDYEAVSEELE
                     AALGTPLDYMERWEDERFKVFPELALTLGDLSFTAVWLAGSDSLVTHSLYANDLSINH
                     VIYINPLMDIFLNFDLNEEFGEDADEEAAPTDAPVVPLEEAEVGEESDFGDEPPEASL
                     EPDPAQADPNFTE"
BASE COUNT          721 a          769 c          732 g          801 t
ORIGIN      
        1 tttttttagg cgaaaaaaag ctgctctatg gctcgcttat tcagctagcc ataaagcagc
       61 tggtataacg cttaggggtt accccaccct ctgcagcccg tttactttat ccgtttcaaa
      121 agcgggttct cacaggctct cctgccggct ttagccattg gatgccagtc gcctccgacc
      181 ttcacgcgga cgatatctgc agtgaagtcg ttgttggtgc cgcacttggc gcagttggta
      241 aggagggatg atcccacgcc gtagatatcc accggtacct tcagggactc gaagagttgg
      301 atcttgttga catcgaagcc gcctgtgacg gtgatcttca cgccctggca gtactttctt
      361 gcctcttcca gatcctccgc tggaagatcc caagtgaggt aggaagtgtc cagtgcctct
      421 ctcagcttcc agaccattct cggattgacg ccgagatcga gctctttggc acccaaaggc
      481 gagatagact tgtccctcat gttgccacct gtgtccggtc ttactgcaag gagtcggtac
      541 ttttcggctt cctcttcatt gccctgcttc ttgtattcca ggtacttgtc gaacatggct
      601 ttgagaactg ctttggtctc gccgaccacg tcgttgtgga agtcaaccag cgcaatgcgg
      661 gagtagctgc gggggaggac tctggcaaac tgcagcatgg tctctacagt atcacccaag
      721 aagcagcaga tcgctgcatg ggaaattgtt ccgccgcctc ttccgcccca gaactcgccc
      781 tgggcgtcgg tggaaacgaa gatcttcgac tcttttctgc cgtggcggta gttgtaagct
      841 tggactccca gggagaaggc gtagccgccg atggcctgca ttttatagtg gacgtacctt
      901 gcagggaaga acagcacgtc tttgccgttg caggcttcaa ggacatttaa tacgttggta
      961 gcgattctgg taggctctga cagaactccc aagatcactg tctctaggtg gccgatctcg
     1021 cggtagcggc ctctgatctt cattacgggc ttcacaaacg aaggatcccc ttcataggga
     1081 acccagacgc cgtcttccac ggcccaaact tctaggtgat cgtaggtgtc tacccacttc
     1141 ccgtctttat cgaagtatcc cgtacatgac tccaaaatgc tcagggcttc atcggtgcca
     1201 acaataagcg accccggaga gcgcctggtg aagatctgca tctcaacctc aatgttgcct
     1261 gtatcaacat cggagcagtc cacatcttcc agatcgcttc tgccggagaa gcgatagccc
     1321 tcttcagcta gctgctgaag aatcctgatt tggtttagga agtaagcgtc ggtataccag
     1381 ccttttctaa ttctctcttt atctatttga aacagcgatg ctggtagcct cttgtggtcg
     1441 aaaaagctca acattaatcc ctccagatcc tgtagtactc tttgttcaaa atgttacttt
     1501 tttgcctggt ttttaatatt atatcattaa gaacctggtt ggtcatattt tgccgcaaat
     1561 ggttattttt ttaacaaacc cgcgcgtttt ccttttgaag cctcgggagc agagtccctg
     1621 cttttttcgt ctaattaaca gactcacatc taaaaaggag aacgtattat gataaagcag
     1681 atctggtggt ttttgattat actgatcatt ggaggtgcag ctatgggtct cagccgactg
     1741 ctgccgcaga gaaaaatgac cgatttagaa tatatctata ttgaagatgc agaaagcctc
     1801 cccagctatg ccgcagatga tgaagagctt ctccgggatc ttgaagacgg accggttctc
     1861 atcatttctc ggggcgaaaa aagcaccggc ggctatcaca ttgagctgat cgcaagcgaa
     1921 ttagaagacg atcacctgga gattgaggtg acgacctccg atcccaagcc tgggtctttt
     1981 tccatcatgg tgatcaccca cccctacgta catgtaaaaa cagaaggtgc aatgacatac
     2041 tcggttactg taaacggaag tgtggctgta gagcggcaaa gcctactgta ggctttgccc
     2101 ttttctatac tgacgtcctc tcgtaactct gagagcccag tttagtctta cgaggcaaaa
     2161 cctaaaaact ccactggttt tggtacaagc acttttttct acgcattttt cttcagtgtg
     2221 atatcattag gttaagacgc gagagacctt ttttgggggt gggaacatgt ttaaacgcgc
     2281 agctgcacta tttctattcc ttctactgtc ttttgtggtg tgtgcagaga gcttgtccgg
     2341 ctttccctgg aacgcaggag tggatgaagt taaagctcag gaagagatcg atcctctgta
     2401 cgaaaccagc agcttcatcg tctacgagac aacgctcttt ggcagaaagg tgcagaagat
     2461 ctacagcttc gacgaaaacc ggctcaccag catcatttac atcttttacg agctccccag
     2521 cgagctgaag ggctatcttc atgactacga agcagtaagc gaggagcttg aagccgcttt
     2581 aggaactcct ctggactata tggagcgctg ggaagatgag cggttcaagg tgtttccaga
     2641 actggcgcta actctcggcg acctgtcctt cacagccgtt tggcttgcag gctccgatag
     2701 cctagtaact cactcccttt atgctaatga tctctctatc aaccacgtca tctacatcaa
     2761 tcccctaatg gacatcttcc ttaacttcga tctaaacgaa gagtttggag aagacgctga
     2821 tgaagaagcc gctccgacgg acgctcctgt cgtgcctcta gaagaagcgg aggtcggtga
     2881 agaaagcgat tttggggacg aaccgccgga agcaagctta gaaccagacc cagctcaggc
     2941 agatcccaac tttacagaat agaaactcaa gggagtgttg caaaactact aaataagtag
     3001 ggagcaacgc tccctttttt gtt
//
LOCUS       DAOSDA010000213         3007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21061_length_3007_cov_2.886518, whole genome shotgun sequence.
ACCESSION   DAOSDA010000213 DAOSDA010000000
VERSION     DAOSDA010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21061_length_3007_cov_2.886518"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..2446
                     /gene="priA"
                     /locus_tag="PLW82_05590"
     CDS             50..2446
                     /gene="priA"
                     /locus_tag="PLW82_05590"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOJ46781.1"
                     /translation="MIAKIVLRTPPVLDKAYDYLVPENLKVKIGQRVIVPFGKAKKEG
                     IVVGLASSSEFPDLKKIHSIMEEEPLLSEELIAMAKWMHKRFLSPLGACVFAMIPAGM
                     DLVCTRYYFLSSENWDERIRDKDPGLFLSADLDRIFDEVSVGSTLLGFDRDVIDRLLK
                     LDLIYFKEHWHQKGVKPKTKKGYVLIDKTAATTPKQKKVVEVVQSGAVFRDEILEEAG
                     VTSAVLKKLVENGVLKEVAMPVRRDPLALRKIGRTSSLTLNREQEEVLRDILQEPGKD
                     ADRKDTYLIFGVTGSGKTEIYLQAIAHCLERGKNALVLVPEIALTTQMVDRFVSRFGD
                     QVAVLHSALGKGERYDEWQRVKNGEASVVIGARSAVFAPLDNIGIIVLDEEHEDSYAQ
                     DDKIPYYHARDLAQFRARWHGVKLVLGSATPSLESYKKALDGVYGLGVLLNRATSQTL
                     PKVHIVDMREELKSGNRTIFSGPLKAALQTHLEAGRQAILFLNRRGFSSFVFCRECGL
                     VLTCTNCDVALTYHLQNNKLTCHYCNAESVPPRKCPHCGSPYIKHFGIGTQRIEAEIL
                     AEFPGVKTLRMDIDTTRTKNAHQKILDQFRDHKADILIGTQMVAKGLDIPNVTLVGVV
                     AADTSLNIGDYRAYEKTFQLLTQVSGRAGRGDYPGEVVIQTYTPEHYAVRYAKEHDYL
                     SFFKMENEFRRRAKYPPYSHFAQILLEGEDQKKVSSGLQACHKEFSGCLPAEVQVIRA
                     GSAPLKKLKKMYRYNILLRSASRELLEEFLRERDGDFVNISKKYGLKVTARIDPGSTL
                     "
     gene            2467..2928
                     /gene="def"
                     /locus_tag="PLW82_05595"
     CDS             2467..2928
                     /gene="def"
                     /locus_tag="PLW82_05595"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOJ46782.1"
                     /translation="MAILPIRKVGDDVLRQVAKPVDKITKKRKKLIKDMIETMYDADG
                     IGLAAPQIGVSERIIVVDVGEGPFALINPEIKEASGSEIDVEGCLSIPGVRNYVKRNA
                     NVVVDALDEDGKPVRIDAEGLLARCLQHEIDHLNGVLFTDKIEQVEDKEKV"
BASE COUNT          867 a          656 c          809 g          675 t
ORIGIN      
        1 gggaaagaac tgacaaagcg gaaattctgg cgagatacaa gtgaagacta tgatagcaaa
       61 aatcgttttg agaacgccgc cggtgctgga caaagcctac gattatctgg tgccggaaaa
      121 cctaaaggta aaaataggtc aaagggtaat cgttccgttt ggcaaagcca aaaaagaagg
      181 cattgtcgtg ggactggcct cttcttccga gtttcccgat ctgaaaaaga ttcactccat
      241 tatggaagaa gaaccgctcc tgagcgaaga gctgatcgcc atggccaaat ggatgcataa
      301 gcggttcctt tccccgctgg gggcctgtgt ttttgctatg attcctgcgg gtatggacct
      361 tgtctgtacc cgttattact ttctaagtag cgaaaactgg gatgagcgta ttcgagacaa
      421 ggatcctggg ctgtttcttt ctgctgattt ggatcgcatt ttcgacgaag tgtctgtagg
      481 ttcaacttta ctgggcttcg atcgagacgt catagatagg ctgctcaagc tagacctaat
      541 atacttcaaa gaacactggc atcaaaaagg cgtaaagccc aagacgaaga aaggctatgt
      601 cttaatagac aaaaccgccg caactacgcc taaacaaaaa aaggttgtag aagtggtcca
      661 gtcgggagcg gtttttcgag acgagattct cgaggaagcc ggcgttacct ctgcggtact
      721 gaaaaaattg gttgaaaacg gcgttcttaa agaagtggca atgccggtaa gaagagatcc
      781 cctagcgttg agaaagatcg gcagaacgag ctctttaacc ttgaaccgcg agcaggaaga
      841 agtcctacgg gatattctgc aggaaccagg aaaagatgca gaccgaaaag atacctatct
      901 gatctttggt gttacaggaa gcggcaagac cgagatctac ctgcaggcca tcgcccactg
      961 cctggaaaga ggtaagaatg cactggttct cgtgccggag atcgccctca ccacccagat
     1021 ggtggaccgc tttgttagca ggtttggcga tcaggttgca gtccttcaca gcgctttagg
     1081 gaaaggcgaa aggtacgacg agtggcagcg ggtaaaaaac ggcgaagcta gtgtagttat
     1141 tggagcaaga tccgcggttt ttgcgccgct agacaacatc ggcatcatcg tcctcgatga
     1201 agagcacgaa gacagctacg cacaagacga caagattcca tactaccatg cgagagatct
     1261 ggctcagttt agagctaggt ggcacggggt aaaactagtt ctgggaagcg ctacgccttc
     1321 tcttgaaagc tataagaagg ctttagatgg agtgtacggt cttggagtgc tgttaaatcg
     1381 ggcaacgtct caaaccctgc ccaaagtgca tatagtcgat atgagagaag agttgaaaag
     1441 cgggaacagg actatttttt ccggtccttt aaaagctgca ctgcaaaccc acctcgaggc
     1501 gggcaggcag gcgattttgt ttctcaacag gaggggcttt tccagctttg tgttctgtag
     1561 ggaatgtggt ttggttttga cctgcaccaa ctgcgatgta gctttaacgt accacctgca
     1621 aaacaacaaa ctgacctgcc actactgcaa tgctgaaagt gttccgccga gaaagtgtcc
     1681 gcactgcgga tcgccttata tcaagcactt tggtataggc actcagcgaa tcgaggccga
     1741 gatcctagca gagtttccgg gagtcaagac cttgagaatg gatatcgaca ctaccagaac
     1801 gaaaaacgct caccagaaga ttttggacca gtttagggac cacaaagcag acattctcat
     1861 cggcacgcag atggtggcca aaggcttgga cattcccaat gttacactgg taggggtggt
     1921 ggcagcggat acatcgctga acatcggtga ctacagagcg tatgaaaaga ccttccagct
     1981 cctaacgcag gtttccggca gggcaggaag gggcgactac ccgggagaag tggttattca
     2041 gacctacacc cccgaacact acgctgtgcg atatgccaaa gaacacgatt atttgagctt
     2101 tttcaagatg gaaaacgagt ttcgcaggag ggcaaaatac cctccataca gtcactttgc
     2161 ccagattttg cttgagggcg aagatcagaa gaaggtaagc agtggccttc aggcctgtca
     2221 caaagagttt tctggatgtc ttcccgcaga ggtacaggtc attcgagcgg gcagcgcacc
     2281 attaaagaag ctgaagaaga tgtatcgcta caatatcctc ctccggtcag cttcacgaga
     2341 acttttagaa gagtttctgc gagagaggga tggcgatttt gtaaatatca gcaaaaaata
     2401 cggcttaaag gtaacagcca ggatcgaccc cggctcgacc ctgtaaaagc gaaagttggt
     2461 gaaactatgg caattctgcc catcagaaaa gtaggagatg acgttctcag gcaggtagca
     2521 aaacctgttg acaagatcac aaaaaagaga aaaaaactga ttaaagacat gatcgagacg
     2581 atgtacgatg cagacggcat cggtcttgcg gctcctcaga taggcgtctc ggaaaggatc
     2641 atcgtagttg atgtgggaga gggtcctttc gccttgatca atcccgagat taaagaagcc
     2701 agcggcagtg agattgatgt tgaaggatgc ctttccattc cgggagttag gaactacgtc
     2761 aagcgcaatg caaacgtggt ggttgatgct ttagatgaag atgggaaacc tgttcgcatt
     2821 gatgcagagg gtcttcttgc acggtgccta cagcatgaga tagaccatct caacggggtt
     2881 ctgtttacag acaaaatcga acaggtagaa gacaaagaaa aggtgtgacg ctatagccgt
     2941 gaaaatcatt tttatgggaa caccggagtt tgctgcgcac cagctggata aactgaagga
     3001 tgtacac
//
LOCUS       DAOSDA010000214         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21164_length_2998_cov_3.139314, whole genome shotgun sequence.
ACCESSION   DAOSDA010000214 DAOSDA010000000
VERSION     DAOSDA010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21164_length_2998_cov_3.139314"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLW82_05600"
     CDS             <1..538
                     /locus_tag="PLW82_05600"
                     /inference="COORDINATES: protein motif:HMM:NF028444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5109 domain-containing protein"
                     /protein_id="HOJ46783.1"
                     /translation="SFYGFYIPHELYPSSSVLPQNYMNFFRDLTIGLQEMGKPVSIAP
                     YFGGNMMPKSHGKYWAAFFEQVPLDVLMLQDGVGCHRLPLEQIPRYYEEVYKVCKEYG
                     VEFWSDLEVFNQLPGEGFSAEPADFERVQRQVITQTPTVDKIVIFDWPHYMSPNRNAK
                     AKALYDAYVAWIESEPQL"
     gene            complement(608..1078)
                     /locus_tag="PLW82_05605"
     CDS             complement(608..1078)
                     /locus_tag="PLW82_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46784.1"
                     /translation="MKKLVIALFLLLLIVPAFAKQNVGFGFTISGEGSGIFFMIPSKT
                     KINPEIVTSGYITEYHYDLSLGGRVLYDLGQKGVVSRYTGAAAGLTLTSKYNMDTDTY
                     AATVSYWGQLVLGTKVSLRESFLKAPVKIRGEVGFSLNDIWGRTHVGVGLEYEF"
     gene            complement(1097..1555)
                     /locus_tag="PLW82_05610"
     CDS             complement(1097..1555)
                     /locus_tag="PLW82_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46785.1"
                     /translation="MKYLRAIALCLLLSMAVLGAQSFGFGSSMAFSADSSTLQGIVFD
                     IPTKGPWNAHVVGMLDVGTSTEFNLGFKINRDMGKVYNIMRYVGVGMGISQDWAKDLR
                     LAGQAQVGIKIPGFVEGFWFDLEVGFSLSANKAGKLRGGAFVSGGTSYSF"
     gene            1788..2435
                     /locus_tag="PLW82_05615"
     CDS             1788..2435
                     /locus_tag="PLW82_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46786.1"
                     /translation="MFSSSHAVGDDHLLLEEIKNQLDRLEASLKEEQEKTERALLDLK
                     SMLQSTQQNNGRTAESQGSGGGGEQDGVQGLLSLAENLPAEFANSLPALKPLLENPQK
                     LLSSPIMKLLPLLANDTFAKSFEVFQKIQEGKLPAPSEILPIARTFLGSPSDRAFTLQ
                     NSDPKLQKVQTIRRDLEKNGLEFAKTLEKLQEMNKEALALVELMEILLMRKQQVR"
     gene            complement(2465..>2998)
                     /locus_tag="PLW82_05620"
     CDS             complement(2465..>2998)
                     /locus_tag="PLW82_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009226296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOJ46787.1"
                     /translation="SMIDAIKEYAGVDLSGQMSDEEALALAKEHHIEVEASWSYGHVV
                     NAFFEKYCEDKLIQPTFIYGHPVEISPLAKKDEKDPRFTQRFEVFIVQREHGNAFSEL
                     NDPIDQKERFMQQLKEREAGNEEAHVMDEDFITALEYGMPPAGGLGIGVDRMVMLLTD
                     QPSIRDVILFPHMKHKD"
BASE COUNT          808 a          785 c          678 g          727 t
ORIGIN      
        1 aagcttctac ggtttctaca ttcctcacga gctgtatcct tccagttccg ttctgcccca
       61 gaactacatg aacttcttca gagacctgac tatcggtctt caggagatgg gtaaacctgt
      121 ctctatcgct ccgtacttcg gcggtaacat gatgcccaaa tctcacggca agtactgggc
      181 tgctttcttc gaacaagtgc ccctcgatgt tttgatgctg caagacggag taggctgcca
      241 cagactgcct ttggagcaga tcccaaggta ctacgaagaa gtctacaaag tttgcaagga
      301 gtacggcgtg gagttctggt ccgacctgga agtcttcaat cagctgccag gagaaggttt
      361 cagcgccgaa cctgcagact tcgagagggt gcagcgtcag gtaatcaccc agacgccaac
      421 tgttgacaag atcgtaatct tcgattggcc tcactacatg agccccaaca gaaacgccaa
      481 agcaaaagcc ctctacgacg catacgtggc ttggatagag tccgaacctc agctataagc
      541 agtaaaaatt agaccgcttg cgggtaaatc cccgccagcg gtcttttctt tctttagaag
      601 gcaactctta aaactcgtat tccaaaccaa cgccgacatg ggtgcgaccc cagatgtcgt
      661 taagactgaa gcctacttcg cctctgatct taaccggcgc cttgaggaag ctttctctca
      721 gagaaacctt cgtacctaaa accagctgtc cccaatacga cacagttgct gcgtaggtgt
      781 cggtgtccat attgtacttg gaagtgaggg tgagacctgc ggcagcacca gtgtatcgac
      841 tgacaacacc tttctgtccc agatcgtaca gcactcttcc accaagactc aggtcgtagt
      901 ggtactcggt aatgtagccg gaggtcacga tttcaggatt gatcttagtc ttgctgggaa
      961 tcatgaagaa gatcccagag ccctctccgg agatggtaaa gccaaagccc acgttctgct
     1021 tggcaaaggc aggcacaatt aaaagtaaaa gaaacaacgc tatgactagt tttttcatga
     1081 tctgttgcac ctcccgttag aagctgtagg aggtgccgcc tgaaacaaag gcgcctcccc
     1141 gaagctttcc tgccttattc gcgcttaggc taaacccaac ctcaagatcg aaccaaaatc
     1201 cctcgacaaa acctggaatt ttgataccta cctgagcttg cccggcgagc ctaagatcct
     1261 ttgcccaatc ttgtgaaatc cccatgccca caccgacata acgcatgatg ttgtacactt
     1321 tacccatgtc tctgtttatc ttaaaaccga gattgaattc agtgctagtg cctacatcca
     1381 gcatgccgac tacatgagca ttccacggac ccttggtcgg aatgtcgaag acgattcctt
     1441 gaagagtgga agaatcggcg gaaaaagcca tgctcgaacc aaacccgaaa ctctgcgcac
     1501 ctaaaactgc catgctgaga agaaggcaga gtgcaatagc tctaaggtat ttcatctgct
     1561 cacctcttta tctagttgta ccatgatcta gtaagtagtt ctatatttaa cattaccacc
     1621 cttttggcaa aattttaagg ggaaactgct acttaatcgc cttcctagag agaaacgcaa
     1681 caaatttgga gattcccacc gctattttta ccaaatcggc tctacctgac atatctatag
     1741 ctagtaagag agtcttgatg caaaagttct attaaggggg aacggcgatg ttcagtagca
     1801 gccacgctgt aggcgatgat cacctgctgc ttgaggaaat caaaaatcag ctggatagac
     1861 tggaggcttc cctaaaggaa gagcaggaga aaacggaacg agctctcctt gatctcaaaa
     1921 gcatgcttca gtcaacgcag caaaataatg gcagaacggc cgaaagccag ggttccggag
     1981 gagggggcga acaagacggc gtccagggac tcctcagctt ggcagaaaac ctgcctgcag
     2041 agtttgcaaa cagcctcccg gctttgaaac cgcttctgga aaacccgcaa aagcttctct
     2101 caagcccgat tatgaaactc ctgccccttc ttgcaaacga tacctttgca aaatccttcg
     2161 aagtctttca aaagatccaa gagggtaagc tcccggcacc tagcgaaatt ctccccatcg
     2221 ccagaacgtt tttaggcagc ccctctgaca gggcatttac cctccaaaac tcagatccca
     2281 aactccaaaa agtgcagacc ataagacgag acctggagaa aaacggactg gagtttgcca
     2341 aaaccctaga aaagctgcag gagatgaaca aagaagcact agcccttgtg gagctaatgg
     2401 agatcctctt aatgcgaaaa cagcaggtac gctaggtgcc tgctgtttaa atctctttat
     2461 tcttttaatc tttgtgcttc atatggggga atagtataac atctctaatc gaaggctgat
     2521 ctgtaaggag catcaccatg cggtccacgc caatacccaa tcctccagca gggggcatgc
     2581 cgtactctag agctgtaatg aagtcctcgt ccatgacgtg ggcttcttca tttccagctt
     2641 ctctttcctt caactgctgc atgaaccgct ctttctgatc gatggggtcg ttcagctcgg
     2701 agaatgcgtt gccgtgctct ctctggacaa taaatacctc aaatctctgg gtgaatcgag
     2761 gatctttttc gtccttcttc gccagaggag agatctccac aggatgtccg taaatgaagg
     2821 ttggttgtat cagcttatct tcgcagtact tctcaaagaa cgcattgact acgtgtccgt
     2881 aagaccaaga tgcttcaacc tcgatgtggt gttctttagc aagtgcaaga gcttcttcgt
     2941 cgctcatctg tccgctgagg tcgacaccgg catactcctt gatggcatcg atcatgga
//
LOCUS       DAOSDA010000215         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21167_length_2998_cov_2.776758, whole genome shotgun sequence.
ACCESSION   DAOSDA010000215 DAOSDA010000000
VERSION     DAOSDA010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21167_length_2998_cov_2.776758"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="PLW82_05625"
     CDS             <1..1210
                     /locus_tag="PLW82_05625"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOJ46788.1"
                     /translation="LIVQKYGGTSVADATRVRDVAERVLYTQQKGNQMVIVVSAPAGK
                     TDELLALARGVAKHPNPREVDMLLATGEQISIALLAMAIADLGGKAISLTAAQVGIKT
                     DGTHQKAKITEISLKRIKEELEKGQVVIVAGFQGIDEKHDYTTLGRGGSDTSAVALAA
                     ALNADMCEIYTDVDGVYTADPRLVPNAKKHDEISYDEMLEMASLGAKVLHLRAVELAK
                     KYNVPLHVRSSFNENPGTVVKGGENMEQGRIVVGVAHNSKISKLTIARIADKPGVAAM
                     IFSKLAAADINVDMIVQSAGKDGLADISFTVSKDDADRAEEVARSMVEVLSAESVERD
                     DNQAKVSIVGTGMLTNPGVAAAMFETLAQNNINIEMISTSDISISTVIPGELVEKAVQ
                     ALHSAFELDK"
     gene            1275..1481
                     /locus_tag="PLW82_05630"
     CDS             1275..1481
                     /locus_tag="PLW82_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOJ46789.1"
                     /translation="MQTDIRIDGMNCMGCVAALLAKLRTLDGVEDADIDLASSVAKVT
                     FNEQLVNLTTIKQAIRDSGYNVLS"
     gene            complement(1551..2636)
                     /locus_tag="PLW82_05635"
     CDS             complement(1551..2636)
                     /locus_tag="PLW82_05635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ46790.1"
                     /translation="MECLLLDSSVFKYAMDNLPASVLLTDKQGHIVYVNERALNMYEC
                     KHINEMMLDLFSLFALEEEQKCFAEVFPHSTASRKEPGHFVCKHKTVKGRIIDVDLKW
                     QPCDFSSTNLIFVVEDITEKRKMEEDLKRLNKLESTVKKLKKASTKLKKYQQKLLAEN
                     SFLKAKEEANEALLSELASTNDHLERLALEDSLTGVYNRRYFTYSLEIELIRAMHLKE
                     PLTLLLIDLDYFKKINDTHGHTIGDEVLKIVATSIQDVVGPKNILCRYGGEEFGVISL
                     GTDLEAGRRLALKIADYLANNPIEVEQHKVTVTVSIGVSSVSPAKWKAPMDAILDYLL
                     KTADSALYKAKSAGRNTVEVYYKRPMV"
BASE COUNT          816 a          654 c          737 g          791 t
ORIGIN      
        1 cttaattgta cagaaatatg gaggaacctc tgtagcagac gcaacgaggg ttcgcgatgt
       61 cgcagaaaga gttttgtaca ctcagcagaa gggaaatcag atggtcatcg tcgtctctgc
      121 cccggctgga aagaccgatg agctgttggc gctggccagg ggagttgcga aacaccctaa
      181 tccccgtgaa gtagacatgc ttcttgctac tggggaacag atctccatcg cgcttctggc
      241 aatggccatt gccgacttgg gtgggaaagc gatctctctg accgcagctc aggtaggcat
      301 caagactgac ggaactcatc aaaaagctaa gatcactgag atttctttaa aacggatcaa
      361 ggaagaactg gaaaaaggtc aggtcgttat cgttgccgga ttccaaggta tcgacgaaaa
      421 acacgactac acgactcttg gccgcggagg ttccgatact tctgcagtag cgctagccgc
      481 tgccttaaat gcagatatgt gtgagattta cacagacgta gatggtgtgt acactgcaga
      541 tccgagactg gtaccgaatg cgaaaaagca cgatgagatc tcctacgatg aaatgctcga
      601 gatggccagc ctaggagcaa aagtactgca ccttagagcc gtagagctgg caaagaaata
      661 taacgtaccg cttcatgtgc gttccagttt taatgagaac ccaggcaccg ttgttaaagg
      721 aggggaaaac atggagcaag gccgcatagt agtaggtgta gcgcataaca gcaagatttc
      781 caagttgaca attgctcgaa tcgctgacaa accgggcgta gctgcgatga ttttctcgaa
      841 gctggcagct gcagatatca acgtcgatat gatcgtacag agcgctggaa aagatggatt
      901 ggcagatatt tctttcaccg tgagtaagga cgatgcggac agagctgagg aagttgcccg
      961 ctcgatggtt gaggtgctct ctgctgaatc agtggaaaga gatgacaacc aagctaaggt
     1021 atctatcgtt ggaactggaa tgcttacaaa tccaggcgtt gcagcagcga tgtttgaaac
     1081 cttggcgcag aacaacatca atatcgagat gatcagtaca tcggatatca gcatttccac
     1141 agtcatcccc ggtgagctcg ttgagaaggc ggttcaagct ctgcacagcg cttttgagct
     1201 ggacaaataa ccttcataaa ggtctgcatt tcgtgtaatg ctaatcagag gactagcact
     1261 taggagtgag cactgtgcag acggacatca gaatagacgg aatgaactgc atgggatgcg
     1321 tcgcagctct cttagcgaaa ctgcggacct tagatggcgt tgaggacgcc gacatcgatc
     1381 tggcgtctag tgttgcaaag gtcacattta acgaacagct ggtgaactta acgacgatta
     1441 agcaagcgat cagagattca ggttacaacg tcttaagcta aaaaaaacac ccgttagggt
     1501 gttttttgtt acagttaagg cgcccagtgg tttttagcta cttccaagct ctacaccatt
     1561 gggcgcttgt aataaacttc tacagtgttt ctgcctgcag acttggcttt gtaaagggca
     1621 ctgtctgctg tttttaaaag gtaatctaaa atcgcatcca ttggggcctt ccattttgca
     1681 ggagagaccg agctcacgcc gatacttaca gtaacagtca ctttatgctg ttccacctca
     1741 atcgggttgt ttgccaggta atcggcaatt ttcagagcca gccgcctgcc cgcctcaaga
     1801 tcggtgccca gggagattac tccgaactcc tcgccaccgt agcggcagag gatgttcttg
     1861 ggaccaacaa catcttggat agatgtggca actatcttca aaacttcgtc cccaattgta
     1921 tgaccgtggg tatcgttgat cttcttgaag tagtccaagt cgatcaggag gagagtaagt
     1981 ggctctttaa gatgcatggc tctaattagc tcaatctcca aagaataagt gaagtaccgc
     2041 cggttgtaga ctccggttag tgagtcttcc aatgctagcc tctcgaggtg atcgttggtt
     2101 gatgcaagct cgctcaaaag cgcttcatta gcttcttcct tggctttaag gaaagagttt
     2161 tctgcgagga gcttctgctg gtacttcttc agtttagtgg aggctttttt tagcttttta
     2221 acggtgcttt ctagtttatt aagtctcttg aggtcttctt ccatctttct tttttcggta
     2281 atgtcttcaa cgacaaaaat caggtttgtg gaagaaaaat cgcacggttg ccacttcagg
     2341 tctacgtcaa ttatgcgacc cttaactgtc ttgtgcttac atacaaaatg accgggctcc
     2401 ttgcgggacg cagtactatg tgggaagact tcagcgaaac atttttgttc ttcttcgagg
     2461 gcgaacaagc tgaagagatc gagcatcatt tcatttatat gtttgcattc gtacatattc
     2521 aaagctcttt cgttgacata gacaatgtgg ccttgtttgt cggtaaggag aaccgacgca
     2581 ggaaggttgt ccatcgcgta tttgaaaacg gaactatcta gtagcaaaca ttccatagtt
     2641 acaccgcgaa tcagctaacc tcaaactcaa cctaaagccc gttgcaaggt ctttaggtaa
     2701 aactataaga gtggggcgcg gatagcttcg gtattcacca cctttcttgc gaattttgct
     2761 ccagcccgct tacgaacttc atcgctttat tcacaagtta gttacaaagg aagaaactcc
     2821 ttcaatttaa acttaagttc gtgagacttt agtcctattt tgttgcaggc atagattatt
     2881 ataatatgga aacactttaa cagggaacca gaaataggaa attgttttgg ctgggaaaat
     2941 ttactgtcaa tacccgctga aaatgtcagt gagtaatcga gactattacc cgatgaat
//
LOCUS       DAOSDA010000216         2984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21315_length_2984_cov_2.682827, whole genome shotgun sequence.
ACCESSION   DAOSDA010000216 DAOSDA010000000
VERSION     DAOSDA010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21315_length_2984_cov_2.682827"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PLW82_05640"
     CDS             <1..624
                     /locus_tag="PLW82_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46791.1"
                     /translation="YGASVYVSVNVAEIDARSSYLSLTDVKTGKIKAADSYVQLTNST
                     PEIIETTRCQLEIAQAEAKKDQIILDATETVVNVSSVALVKGNAQNSTVSLEYVDEVD
                     LTVTGGTLRVRRTENNLGLHLEGTNAYLELGDHFSCITAEESEVTIKMLRTDAELLVE
                     TTGTEISTNVVGAEGPNFSYVRGKDAKEQTLKTQGGILRLYSPFVSM"
     gene            967..1485
                     /locus_tag="PLW82_05645"
     CDS             967..1485
                     /locus_tag="PLW82_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOJ46792.1"
                     /translation="MAKIAMIIAKSEFRDEEYLHPKEEFLKNGHEVITASSTLGECTG
                     RFGAKVTPDIDFKELTTDDFDALVFVGGGGSSEYFDSPIAHGLAREAYRNGKIVSAIC
                     IAPVILSRAGLLQGKKATVYPDGENDLVKGGAFYTGNPVEVDERIVTGNGPAAARDFA
                     KAVMEALEALQK"
     gene            1757..2794
                     /gene="spoIID"
                     /locus_tag="PLW82_05650"
     CDS             1757..2794
                     /gene="spoIID"
                     /locus_tag="PLW82_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HOJ46793.1"
                     /translation="MDKRNAVLTAVWFAVGVLLICCIVLAVGTKKERALLDRKVRLYV
                     YGKGLLELDLEEYLVGVVAAEMPASFHAEALKSQAVAARTYTLKRLERIARTGSPYPE
                     NADITNLPQEGQAWLSEDERKQKWQDDFAQYEKKIRDAVYSTKGEVLVYDGALIEALY
                     YSTAGPYTEDSTAVFGAYYPYLKAKPNPYDKHSPRYTDAKTVSIAELEKAFGVSLDVK
                     NLVLARNLPQGPEVFAAATLLEPWSNPDPTASPIKILSRNRSGRVELVQIGSKVVRGV
                     DVRTKLGLRSTNFTFEVSANGNYITFQTVGFGHGVGMSQYGADGMAKEGFDYKEILSF
                     YYPGTQLVLAR"
BASE COUNT          860 a          662 c          809 g          653 t
ORIGIN      
        1 tacggtgcgt cggtttacgt aagcgtcaac gtagcggaga tagatgcaag aagctcatat
       61 ctgagtctaa cagatgtgaa gaccggtaaa ataaaggcag cagattccta tgtccaactg
      121 acaaactcaa ctccggagat catcgagacc actcggtgcc agctggagat agcccaagct
      181 gaggccaaga aggatcaaat catactggat gcaacagaga cggttgtcaa tgtaagctca
      241 gtggcacttg taaaggggaa tgcgcagaac tctacggtga gtctcgagta cgtcgacgaa
      301 gtggacctta cggttacagg cggcaccctc agagtgagga gaactgaaaa caacctcggt
      361 ttacacctgg agggaaccaa cgcttacctc gaactgggag accatttttc ttgcataact
      421 gccgaagaat ctgaagtcac gataaaaatg ctccgcacag acgctgagct cttagttgag
      481 actacgggca cagagatctc cacaaacgtt gtgggtgcag aaggaccgaa cttcagctac
      541 gtacggggca aggatgccaa agagcagacg ttaaaaaccc agggcggcat cttgcggttg
      601 tactctccat ttgtttccat gtaaaggcaa aaagcagcgg acgcgaaaaa cgcatcagct
      661 gcttttttgt ctgctcctga ccacatcaaa gcaaaggccc actggaaacg ttttgcagga
      721 gatgtctttt tttgcagcaa agtgctataa tagataaaaa agagcaaccc acaggttgaa
      781 aaacctgtgg gtgtgcaggc aaccaatcaa caaacaagca tattgattag cgcctcagta
      841 ttatcatgac tttttttgcc ggttttattc tggcaaattc tggaatgcga gttcccgaga
      901 gtttcaaaag aacgggaagg ggaaaatgga acaagaagta caaaataaga tgtagggggc
      961 gatcatgtgg caaagatagc gatgattata gcaaaaagtg agtttcgcga tgaggagtac
     1021 ctccatccga aagaggagtt cctgaagaac ggccacgaag ttattaccgc ttcatcaacg
     1081 ctcggagaat gcaccggccg ctttggggct aaggtcacac ctgacataga tttcaaagaa
     1141 ctgacaaccg acgacttcga cgctctggtt tttgttgggg gcggaggatc aagcgaatac
     1201 ttcgattcgc ctatagccca cgggctagcc agagaggctt acagaaacgg aaagatcgtt
     1261 tctgcgatct gcatcgctcc ggtgattctg agtagagccg gtctccttca ggggaagaaa
     1321 gcaacagtct accctgacgg tgagaacgac ttggtcaaag ggggagcctt ctatacaggg
     1381 aaccccgttg aagttgatga aaggatcgtt accggaaacg gtccagcggc agcaagagac
     1441 tttgccaaag ccgtaatgga agcgctcgag gcgctgcaaa agtaaactgc tgctgtaagt
     1501 agaataaaga gatgcccaaa ctgagggatg cagagacaag gctttaggcg ccactcctgc
     1561 atccctttac ttttagggca gcgcatggca aaagacccag cgggtgtccc aacagactcc
     1621 cactggcatt ggacttggct atacttaagt ttttgggacc ccggaaatct ctttgtacca
     1681 ctttgcagct caatgcatat tatggttaag tagagtgtag agaggtattt ccaaaacaag
     1741 gaaggggaag ctggttttgg ataagagaaa tgccgtttta actgcagttt ggtttgcagt
     1801 aggcgtcttg ttaatctgct gcattgtgct ggcggtagga acgaagaaag aaagagccct
     1861 tttggacaga aaagtccggc tgtatgtcta cggcaagggc cttttagaac tggatctgga
     1921 agaatacctg gttggagtag tggcggcgga gatgcccgct tcgtttcacg cagaagcgct
     1981 taagagccag gccgtagccg cgaggactta tacgctcaag cggctggaaa ggatagccag
     2041 gaccggttct ccctacccgg aaaacgctga cattaccaac ctgcctcagg aagggcaagc
     2101 ctggttgtcg gaggacgagc gcaagcagaa gtggcaggac gactttgcgc aatacgagaa
     2161 gaagatcagg gatgcggttt acagcaccaa aggagaggtc ttggtttacg atggagcctt
     2221 aatcgaagct ctgtactact ccaccgcagg tccgtacact gaagacagta cggcggtttt
     2281 tggcgcatac tatccttatt taaaagccaa acccaacccc tacgacaaac attccccacg
     2341 ctacaccgat gcaaaaaccg taagcattgc agagctggag aaggcgtttg gagtaagctt
     2401 ggatgtaaaa aacttggttt tggccaggaa cttgcctcaa ggccccgagg tctttgctgc
     2461 tgcaactctc cttgaaccgt ggtccaaccc ggacccaact gcaagcccta ttaaaatcct
     2521 ttcgcgcaac cgctcggggc gggttgaact cgttcagatc ggcagcaagg tggttagagg
     2581 cgttgatgtg cgcacaaagc tcgggctgag gtcgaccaac ttcacctttg aggtcagtgc
     2641 aaacggcaac tacatcacct ttcagacggt agggttcggg cacggagtgg gcatgagcca
     2701 gtacggcgcc gacggcatgg caaaggaagg ttttgattat aaagaaatat taagctttta
     2761 ctatccgggt actcagctag ttttggcacg gtaaaaaagg gaataaacta ggttagaagg
     2821 agtagatgct ctaatgtgga aaatatagac agagcatcta cttctgtgtc gaaaggagga
     2881 gtttttttgt caatagagct aagcagcaac gcccgaacgg ttttagagaa gcgttatctg
     2941 agaaaaaaag atgacggaac ctttgaaact cctgaagaaa tgtt
//
LOCUS       DAOSDA010000217         2968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21478_length_2968_cov_4.220048, whole genome shotgun sequence.
ACCESSION   DAOSDA010000217 DAOSDA010000000
VERSION     DAOSDA010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21478_length_2968_cov_4.220048"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            577..1251
                     /locus_tag="PLW82_05655"
     CDS             577..1251
                     /locus_tag="PLW82_05655"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOJ46794.1"
                     /translation="MVHLGNDWDELLKEEFQKEYYQRLRKILIREYRTKTVYPDMYKI
                     YEALKLTPYKDTKVVILGQDPYHGENQAHGLAFSVQKGVEIPPSLMNIYKELSDDLGC
                     YIPNNGYLAPWAQQGVLLLNTSLTVVANQANSHRGIGWQQLTDAIIQILNKKDAPVVY
                     LLWGNNAKAKRMFLNNPKHLVLTASHPSPLSASRGFFGCRHFSKTNAFLKANGLSEID
                     WQIPNI"
     gene            complement(1308..2846)
                     /locus_tag="PLW82_05660"
     CDS             complement(1308..2846)
                     /locus_tag="PLW82_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOJ46795.1"
                     /translation="MSSNGNEKHEQNLIIWFIPFLLALVGFGFVAVMAEIMSFIWPDI
                     VDLRYYLGFFGVIMVFFGYFATARPESYERKRSRIPEVIALIFLSKLYTAFVLPWDQF
                     VSRLVPAKFLEVNFFFAFILLYFVWVLARSAGNLALTMTQSIVDRELRKQQDESYLGV
                     AVRESITGLKLIKYLFIPMIFVLIFVIGAEQSFQEQVAQSTVFKLKVNLFGGVMAAAT
                     IAMATLVLYLQKNYQWESERAEVEKDLGFKWVIWSLAFVLLFTAVAAFLPGDLSPVYF
                     YIPNAIKALLPFMHRDMPTGPVFQQREKNEPVEPPILDEQTMGKHHSALLTTITATVQ
                     TLIAIAIAVTAFYYLLRVLYENSDELKRLVDNIVRTFKYFINALKSLFIKTETGKVDK
                     KTLAEQVDQFFKRRKITYNKVGINEVRRLYGELLLEAESRGIRREAYATASEFAVVLE
                     NLFPERVQEIVGLTRMYEDARYNKDPDEETSKKAKGLFSVVVQTVRSYTRRSGRKRDA
                     SVED"
BASE COUNT          865 a          735 c          622 g          746 t
ORIGIN      
        1 aacaaattgt actatactta tacaatttgt ccaatctctt agggggtccc taacaagatg
       61 taagatatta acgcagaact tactcctact aatactccga cgatagtaca ggtaaacact
      121 atcgaccaaa tccgtaattg aatcctctca gaaggagtga ttatctgctg ctctttcttt
      181 ttcttgttaa tggtgtacag cagatagacg aacttaacag aaataagtag cgtagcagct
      241 ataataacca gcagtagaaa ataaggcaca ataaccctcc cctttgacgt attcagttta
      301 ctccaagtaa actgtaacaa aaacccagct aggaattaaa taggtatatc ttaccgtttc
      361 actgaatatg acgcgtaaaa aaccgaaaag ttaacagaaa cgtttcacct tgggactaaa
      421 tacctagagc attcggacga aaaaggtaat cccgccgaaa actagaagta acaacagtgg
      481 aaaactgcaa acaaaaaaag cgctcaagga aaagcgccga atctgcgaaa aaaccaccgc
      541 attttatcga agctttacat aggagtgaac tgtaagatgg tacatttggg aaacgattgg
      601 gacgaactgc ttaaagaaga attccagaaa gaatactatc aaagactcag aaaaattttg
      661 atcagagaat accgtacaaa aaccgtctat cccgacatgt acaaaatcta tgaagccctc
      721 aagttgaccc cgtacaagga taccaaagta gttatcttag gtcaagatcc gtatcacgga
      781 gaaaatcaag ctcacgggct ggccttttct gtacagaagg gagtggaaat ccctccctca
      841 ctgatgaata tctacaaaga gctatcagac gatcttggct gctacattcc aaacaacggg
      901 tatcttgccc cctgggcgca gcagggggtg ctgcttttaa acaccagcct tactgtggtt
      961 gccaaccaag ctaactctca tcggggcatc ggctggcaac agctgacaga cgccattatt
     1021 cagattctaa acaaaaaaga cgctcctgtg gtctacctcc tttggggcaa caacgccaaa
     1081 gcgaaacgga tgttcctcaa taaccccaaa catctggtcc tgactgcgtc tcaccccagc
     1141 cctctctctg caagcagagg gtttttcggg tgcaggcatt tttctaaaac caacgccttc
     1201 ctgaaggcaa acggtctttc tgaaatcgac tggcagatcc cgaacattta aggatgagcg
     1261 ctgagccaac cggctcagcg cctttttatg ctttttacaa acgtcttcta atcttcgaca
     1321 ctagcatctc gctttctacc tgaacgcctt gtgtagcttc taaccgtttg gaccactacg
     1381 ctaaacaggc ctttggcttt tttgctggtc tcctcatcgg gatctttatt gtaccttgca
     1441 tcttcgtaca ttctagtgag accaacaatt tcctgaaccc tctctgggaa caggttttcc
     1501 agcaccaccg caaactccga agcagtagca tatgcttctc tgcgaatgcc ccggctttcg
     1561 gcctcaagga gcagctctcc gtacaatctg cggacttcat tgatgcccac cttgttgtag
     1621 gtgattttcc tacgtttgaa aaactgatct acttgctcag ccaaagtctt cttatctacc
     1681 ttacctgttt cggttttgat gaacagactt ttcagcgcgt tgatgaaata cttaaaggtt
     1741 cggacgatat tgtccaccaa acgcttcagc tcatcggagt tttcgtaaag gactctgagc
     1801 aaatagtaga aggctgtgac cgcgatagcg atggcgatca aagtctgtac agtggcggtt
     1861 atagtggtaa ggagtgcgct gtggtgctta cccatcgtct gctcgtctag gattggcggc
     1921 tcaacaggtt cgttcttctc acgctgctgg aacaccggac ctgtgggcat atccctatgc
     1981 atgaaaggca gcagtgcttt aatggcattg gggatgtaga agtatactgg cgacaggtct
     2041 cccggcagga aagcagcgac agctgtaaac agcaggacga aggctaacga ccaaatcacc
     2101 cacttgaaac ccaagtcctt ctcgacttcg gccctttcgc tctcccactg gtagttcttt
     2161 tgtagataca gaactaacgt tgccatagcg atggttgctg cagccataac tccaccaaaa
     2221 aggttcactt taagtttgaa cactgtactt tgggcaacct gctcttggaa gctctgctct
     2281 gcaccgataa caaaaatcag cacgaagatc atgggtatga agagatattt gattagcttc
     2341 aatccggtta tcgactctct gacggcaact ccgaggtagc tttcgtcttg ctgtttcctt
     2401 agttctctat caacaatgga ttgggtcatt gtaagggcca agttcccggc gctccgcgcc
     2461 agaacccaaa cgaagtacag caaaataaat gcgaagaaaa agttcacttc aagaaactta
     2521 gcaggcacca gcctggaaac gaactggtcc caaggaagca caaatgcggt gtacagctta
     2581 cttaggaaaa tcagcgctat aacttccggt atgcgggatc tctttctctc gtatgactcc
     2641 ggtcttgcag ttgcaaagta gccgaaaaag accataatga cgccgaaaaa gcccagataa
     2701 taccggagat caacaatgtc tggccatatg aagctcatga tctctgccat gacggcgaca
     2761 aagccaaaac ccaccaaggc taaaagaaac ggtatgaacc aaataattag attttgctca
     2821 tgcttctcgt ttccgttcga ggacactcat ctcatcctcc tctctaatct gatagagaat
     2881 caaggaggag gtcgtcgacc tgccaagata ctctctgaac ttcgcgctct gaactgtaat
     2941 gatcaccact ttgaagccac tttcaatc
//
LOCUS       DAOSDA010000218         2955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21616_length_2955_cov_2.651379, whole genome shotgun sequence.
ACCESSION   DAOSDA010000218 DAOSDA010000000
VERSION     DAOSDA010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21616_length_2955_cov_2.651379"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2475)
                     /locus_tag="PLW82_05665"
     CDS             complement(<1..2475)
                     /locus_tag="PLW82_05665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOJ46796.1"
                     /translation="MAISLHTHSAYSLLDGASRPYDLIKRGSELGYVAIALTDHDSVS
                     GVWEFQRLALDAGIKPIIGCEVTLEDGSHLTLIAENYTGYQKICSYLNMTDKADKFLP
                     HGEGIFALSGCRNSKLQRLLLRRRYKEALELAKELYLFFEGRFFIEMTNDLLPRTNEI
                     LNSLDELGKELQIPTVASVDVHYACEEDFPVHDSLSCIRLGIKLEDVHPERSFNNAGY
                     LCSPDELVKRFSNFSNALQGMHYLAEICESKPLSPPMLLPSTPGAKEKLREIALAGLE
                     KKYGTGNRRALARLEHELSTISALGYDSYFLLVHDLICEARRRNIAYQGRGSAAGSLV
                     SYCLDLTQVDPIEHNLVFERFLSVERGQQPDIDIDFDWAKRDQLWDYLEGKYGSSHVA
                     SIAAFHCYREDMARNDLLRIGLSPELIPQLTGKMYGLPRHIATHSSGIAIASEDLSRH
                     APLVEAATGRKIVALDKDDIEEIGIVKLDLLSLRTLGALSELNLPIKPEDPATYKMLQ
                     EGKSLGVFQLESPAQRSLQQRLKPERFSDVVASIALIRPGPIRGNMVEPYIKRHNREE
                     EASYPAEELKPYLEKTFGLIVFQEQIIQIVSALGGFSPGESDKLRKMLSNKKRERELY
                     DFHVQFVERAVARGVPEDTAESIFQTLEGFSGYGFCEAHSASFAQTAYKTAFLRCHYP
                     AEFFGALINNQPLGYYLPQSLLWEAKRLGVKVVPLDINKSPHETYGKDSMLYLGLKLI
                     NGFSAKTMDYILENRPLSLEDIKKIPSKERRLLVLSGALDSFGQSRRSLLWEAWGTKP
                     IPGDFTPWERFLREIKVLDLSPSYHVM"
     gene            complement(2489..2716)
                     /locus_tag="PLW82_05670"
     CDS             complement(2489..2716)
                     /locus_tag="PLW82_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46797.1"
                     /translation="MAHIWEKPIAIKDNSFVFRNVRYTIVAELDSWCESGAWWHGEPV
                     LYFSLVKTDPEGLFEIAYTPTKEALLYQSFD"
     gene            complement(2682..>2955)
                     /locus_tag="PLW82_05675"
     CDS             complement(2682..>2955)
                     /locus_tag="PLW82_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46798.1"
                     /translation="CQDKIGRLSLSLALKALFPWETLTCLVWEMPKAHQKSLFDFSLP
                     DVVQSLLDQEKNLTLGISVSRREKVRRSNFIWGYGSGSYLGKTHSN"
BASE COUNT          682 a          722 c          778 g          773 t
ORIGIN      
        1 cattacgtgg taagaaggag acaggtccaa aaccttgatc tctcgcaaaa agcgctccca
       61 aggagtaaag tcgccgggaa tcggctttgt gccccacgct tcccacagaa ggcttctgcg
      121 gctttggccg aaggagtcca gcgcccctga aagcaccaaa aggcgccgct ctttgctggg
      181 gatctttttg atgtcttcca gacttaaggg acggttttcc agaatgtagt ccatggtttt
      241 tgcagagaag ccattgatga gcttcagccc caaataaagc atggagtctt taccgtaggt
      301 ttcatgcggc gatttgttga tgtctaaagg cacaactttt actcccaggc gtttggcttc
      361 ccaaaggaga gactggggaa gatagtaccc gagaggctgg ttgttgatga gagcgccgaa
      421 aaattctgca gggtagtggc accgcaaaaa agcagtttta taagctgttt gggcaaaaga
      481 agcagaatga gcttcgcaga agccgtagcc ggaaaagcct tcgagggttt ggaagatgct
      541 ttctgcggtg tcttcgggca caccccttgc tacggcccgc tccacgaact ggacgtgaaa
      601 gtcgtaaagc tcgcgttccc tctttttatt ggagagcatt ttgcgcagct tgtcggactc
      661 tcctggtgag aagcccccta gagcagatac gatctgaatg atctgttcct ggaagacaat
      721 gaggccgaag gttttttcca ggtagggttt cagctcttct gcagggtagc tagcctcttc
      781 ttccctgttg tggcgcttga tgtagggctc taccatattg cccctaatag gtccgggcct
      841 gatcagcgct atgcttgcca cgacatcgct gaacctctca ggttttagcc tctgctggag
      901 gcttctttgt gcagggcttt ccagctgaaa gactcccagg ctctttcctt cctgcagcat
      961 tttataggtg gctgggtctt cgggcttgat gggcagattc agctcggaaa gggctccgag
     1021 agttcgcagg gaaagaagat cgagctttac aatgcctatt tcttcaatgt cgtctttgtc
     1081 cagtgcgaca atttttcggc cagttgccgc ttccacaagg ggagcgtggc gcgaaaggtc
     1141 ttccgaagct atggcgattc cgctagagtg cgtggcgata tgcctgggca gaccgtacat
     1201 tttacctgta agctgcggaa tgagctctgg agacaagccg attctgagaa gatcatttcg
     1261 agccatgtct tccctgtagc agtggaaagc tgcgatactt gccacatgac tgctgccgta
     1321 cttgccttct agataatccc aaagctgatc tcttttagcc caatcgaaat cgatgtcgat
     1381 gtccggctgc tgcccccgtt caacgcttaa aaaacgctca aacaccaagt tatgttcaat
     1441 gggatctact tgcgtgagat ctaggcagta gcttactagg gatcctgctg ccgatccacg
     1501 tccctgatag gcgatattcc tccttcttgc ctcgcaaatg aggtcgtgga ccagaagaaa
     1561 gtagctgtcg tagcccaggg cggagatggt agagagctcg tgctccaacc ttgccaaagc
     1621 tctgcggttg cctgttccgt attttttctc aagacctgcc aaggcaatct cccggagttt
     1681 ttctttagcg ccgggagtgc taggtaaaag cattggcggc gacaggggct ttgactcgca
     1741 tatctctgcc aaatagtgca ttccctgtag ggcattggag aagttcgaaa agcgctttac
     1801 aagctcatca ggggagcaga gatagccggc gttgttgaag gatctttcag ggtgcacgtc
     1861 ttcgagcttg atgcccagac gaatgcagct caagctatcg tgaaccggaa agtcttcttc
     1921 gcaggcatag tgaacatcca ccgatgccac agtgggaatt tgcagttctt taccgagttc
     1981 atctaggcta ttcaagatct cgttggtacg gggaagaagg tcattggtca tttcgataaa
     2041 aaagcgtcct tcgaaaaaca agtaaagctc ttttgccagt tccagtgcct ctttgtagcg
     2101 tcgccttagg agtagcctct gcagcttgga gttcctgcag ccggaaaggg caaaaattcc
     2161 ctccccatgg gggaggaact tgtcagcttt atcggtcatg tttaagtaag agcagatttt
     2221 ctggtagccg gtgtagtttt ccgctatcag cgtcaggtgg ctgccgtctt ctagcgtaac
     2281 ttcgcagccg ataattggtt tgatccctgc atcaagggca agtctttgaa actcccaaac
     2341 tccagagacg ctgtcgtgat cggttagagc tattgccaca tatcccagct cactgcctct
     2401 tttaatcagg tcgtaaggcc tgcttgctcc gtcaaggagc gagtaggcag aatgagtgtg
     2461 aagtgatata gccataatac aatctccttt agtcaaaact ctgatataag agcgcttctt
     2521 tagtaggggt gtacgctatt tcaaaaagcc cttcagggtc cgttttcacc agagaaaagt
     2581 acagcaccgg ctctccatgc caccaggctc cgctttcaca ccagctgtcc agctctgcaa
     2641 cgatggtgta gcggacgttt ctaaacacaa aactgttgtc tttaattgct atgggttttt
     2701 cccagatatg agccacttcc atacccccag atgaagttgg atctacgcac tttttcgcgg
     2761 cggctgacgg agatgccaag ggtcaggttt ttctcttggt ccagaaggct ttgaaccacg
     2821 tctggaaggc tgaagtcaaa aagacttttt tgatgggcct tgggcatttc ccaaaccagg
     2881 caggttagag tttcccaggg gaaaagagct tttagggcta gagacagaga gagcctgccg
     2941 attttgtcct ggcac
//
LOCUS       DAOSDA010000219         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21660_length_2950_cov_2.936442, whole genome shotgun sequence.
ACCESSION   DAOSDA010000219 DAOSDA010000000
VERSION     DAOSDA010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21660_length_2950_cov_2.936442"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLW82_05680"
     CDS             <1..352
                     /locus_tag="PLW82_05680"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOJ46799.1"
                     /translation="KARENAYAPYSGFKVGAALLARDGSIYTGCNVENASYGLTVCAE
                     RNAVFSGVASGAINFEKIVVAADGDLTPPCGMCLQVLREFGVKTVVLVNLERKWRSYT
                     LDELLPVSFGPENL"
     gene            403..1416
                     /gene="queA"
                     /locus_tag="PLW82_05685"
     CDS             403..1416
                     /gene="queA"
                     /locus_tag="PLW82_05685"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOJ46800.1"
                     /translation="MLVEDFDYHLPEHLIAQKAAEPRDSSRLLVLNRETATLEDRIFR
                     DIVEYLNPGDLLVLNDTRVIPARLLGEKKGTGGKAEVLLLRQKESLLWEALVRPGARL
                     KEGAQIVFGGGLLEAKIEKHLEEGLRIVRFKCDRPFMEVLDELGKMPLPPYIQAELED
                     RERYQTVYSKNPGSAAAPTAGLHFTEELLQKIAAKGVKKAFVTLHVGLGTFRPVSEET
                     VEEHKMHSEMYQVPEATWELIQKTKEAGGRVIAVGTTVVRTLESVGRTGKLFGETDIF
                     IYPGFQFQVVDSMITNFHLPKSTLLMLVSAFADRGMILKAYEHAVREEYRFFSFGDAM
                     LII"
     gene            1428..2549
                     /gene="tgt"
                     /locus_tag="PLW82_05690"
     CDS             1428..2549
                     /gene="tgt"
                     /locus_tag="PLW82_05690"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOJ46801.1"
                     /translation="MQKVQFELLYTSQETRARLGRLHLPHGTIETPVFMPVGTRATVK
                     TLTPDEVKEIGAQIILGNTYHLYLRPGDDLIMRMGGLHKFMNWDRPILTDSGGFQVFS
                     LGKLRKITEEGVFFQSHIDGSKHFFSPERAIEIQQNLGSDIMMAFDECVEYPATREYV
                     EESVKLTYKWLLRCKEAWTNRETQNLFGIVQGGMYKDLREESAKLTVDCDLPGYAIGG
                     LSVGEPLDVLLEVLDHTEPFLPKDKPRYLMGVGSPDIILNSIEMGVDMFDCVLPTRMA
                     RNGTAMTSQGQVTVRNAVYKEDESPLDPECSCPTCKNYTRAYLRHLFNVGEILGLRLV
                     SYHNLYFLTKMVEDAKVAIKQERFREFKKHFLERYYFQS"
     gene            2562..2837
                     /gene="yajC"
                     /locus_tag="PLW82_05695"
     CDS             2562..2837
                     /gene="yajC"
                     /locus_tag="PLW82_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOJ46802.1"
                     /translation="MAETNALGSFAAYLPMLILLAAMYFVLILPQKKQQKQRKELLDS
                     LKTGDEVVTIGGILGRIKKLDEETVLLELSDHVEVRFLRSAVARKKD"
BASE COUNT          784 a          671 c          798 g          697 t
ORIGIN      
        1 aaaagcacgg gaaaatgcct acgcgcctta ctctgggttt aaggtgggtg cggcgctgct
       61 tgcccgagac ggttcgatct acaccggttg taacgtggaa aacgccagtt acggtttgac
      121 ggtgtgcgct gagagaaatg cggttttttc aggtgttgct agtggagcaa ttaacttcga
      181 aaagatcgtt gttgctgcag atggagatct cacacctccc tgtggcatgt gtctgcaggt
      241 tctgagggag ttcggtgtaa aaaccgtcgt gctggtgaac ttggagcgca agtggcggag
      301 ctataccctc gatgagctcc ttcctgtttc tttcgggccg gagaatcttt agttcagaag
      361 ggaggaacaa ttctaacgta gtttgcgttg gagttggcct tagtgttagt agaggatttc
      421 gactaccatt taccggagca tttgattgcc caaaaagccg cagaaccgag agacagctcc
      481 agattgttgg tcttaaaccg cgaaactgcg actttggaag atagaatatt tcgagatatt
      541 gtagagtacc tcaaccccgg cgatctgttg gtgctgaatg acacgcgggt gattcccgca
      601 agacttttag gcgagaagaa gggtaccgga ggaaaggcag aggtactgct tttacgccag
      661 aaggagtctc tactttggga agcgcttgtc agaccgggcg caaggctaaa agaaggtgcc
      721 caaatagtgt tcggcggagg ccttctcgaa gcgaagattg agaagcatct agaagagggc
      781 ctccgaattg taaggtttaa atgcgaccgc ccctttatgg aggttcttga tgaacttgga
      841 aagatgcccc ttccgccgta catccaagcg gaactagagg acagggagcg ctaccaaacc
      901 gtctacagca aaaaccccgg ctctgctgca gctcccactg caggactcca ttttaccgaa
      961 gaactcctgc agaaaatcgc tgcaaaagga gttaagaagg cgtttgtaac gctgcatgta
     1021 ggccttggta cgttcagacc tgtctcagaa gagaccgtgg aagagcacaa gatgcattcg
     1081 gagatgtacc aagtgccaga ggcaacttgg gagctaatcc aaaagaccaa agaagccgga
     1141 ggcagggtaa ttgctgtggg caccacagtg gtgcgcactc tagagtcagt aggtaggacc
     1201 ggaaagcttt tcggtgagac cgatattttt atctaccctg gcttccagtt tcaagtagtt
     1261 gacagtatga ttaccaactt ccatcttccc aaatccacgc tattgatgct ggtttcagca
     1321 tttgccgata gaggtatgat actgaaagct tacgaacatg cagtaaggga agaataccgc
     1381 ttctttagtt ttggcgatgc catgctgatt atctaaccct cgaaggagtg cagaaagtgc
     1441 agtttgaact tctatacaca tcacaggaaa ccagagcaag actaggaagg cttcaccttc
     1501 cccacgggac cattgaaact cccgtgttta tgcccgtagg cactagagcc acggtgaaga
     1561 ccttaactcc cgatgaggtg aaagagatcg gtgcacagat cattctcgga aacacctatc
     1621 atctctacct gagaccaggt gatgatctga ttatgcgcat gggcggactc cacaagttta
     1681 tgaactggga tcggcccata ctcacagaca gcggcggctt ccaggtgttc agcttgggga
     1741 agctccgcaa gatcactgaa gaaggtgttt tctttcagag ccacatcgat ggctccaagc
     1801 actttttttc accggagcgg gccattgaga tccaacagaa cttgggctcg gatatcatga
     1861 tggccttcga cgagtgcgtt gagtacccgg ccacgagaga gtacgtggag gagtccgtaa
     1921 agctgactta taagtggctt ctccgctgca aagaagcctg gacaaacaga gagacccaga
     1981 acctctttgg cattgtgcag ggcggcatgt acaaggatct ccgcgaagaa agtgccaagc
     2041 tcactgtaga ttgcgatctt ccgggatatg ccattggagg gctaagtgtg ggagagccgc
     2101 tagatgttct cttggaggtt ttagatcaca cagagccgtt tctccccaag gacaagcctc
     2161 gctacctcat gggagtgggc agccccgaca ttatcttgaa cagcattgag atgggtgtag
     2221 acatgtttga ctgcgtgctt cccactcgta tggctcgaaa cggaacggcc atgaccagcc
     2281 agggacaggt gacagtaaga aatgctgtct acaaagagga tgaaagtccc ctggatccgg
     2341 agtgcagctg tcctacatgc aaaaactaca ccagagccta cctgcgccac ctgttcaatg
     2401 ttggtgaaat cctcggtctc aggctcgtta gctatcataa tctatacttc ctcacaaaaa
     2461 tggtcgagga cgcaaaagtc gcaattaagc aggaaagatt tagggagttc aagaaacatt
     2521 tcttagaaag atactatttc caatcctaag gaggtaagtt gatggccgaa accaatgccc
     2581 ttggttcctt tgcagcatat ctgccgatgc tcatactgct ggcagccatg tattttgtgc
     2641 tgattctgcc ccagaaaaag caacagaagc aacgcaagga actgcttgac agtttgaaaa
     2701 ccggcgacga agtggtgact atcggcggta ttttgggacg cattaagaag ttggatgagg
     2761 aaaccgttct cctggagcta tccgaccatg tggaagttag gtttctccgg agtgctgtag
     2821 ccaggaaaaa ggactaagac ggacagccat aacttgactt tagaccgctt gctagacagt
     2881 tgcaggttta agttatgcct gaaaaaggcc tgaatttctc aggtcttttt gcgttaaagg
     2941 taggtgaaag
//
LOCUS       DAOSDA010000220         2947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21679_length_2947_cov_4.828492, whole genome shotgun sequence.
ACCESSION   DAOSDA010000220 DAOSDA010000000
VERSION     DAOSDA010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21679_length_2947_cov_4.828492"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="PLW82_05700"
     CDS             complement(<1..132)
                     /locus_tag="PLW82_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_387937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOJ46803.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFVDRDGEV
                     "
     gene            complement(219..1109)
                     /locus_tag="PLW82_05705"
     CDS             complement(219..1109)
                     /locus_tag="PLW82_05705"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOJ46804.1"
                     /translation="MSRKVVIAILVTLLVISVAGNIVLGYLWWEESQPEVVAVVGQDK
                     ITKDDLLDRLLTRNREAQLNTIVEQVLIEQEAKKLGVQVTENDVTRRLQEIKDSLGGE
                     DLYRKFLTDYSISEADLRDSIRHNLTIEKIILFYISDIPETEVVAYYENNKDLFQTPE
                     FRLVRHIFTIDESEAKKAYADLLRGEDFGTVAERYSLDPSTNKEGGLIGYLTRDDEWL
                     GVTETAFQLGKGTFSAPMRSEYGWHLVYVEDIRPASNPDYSEIKDQVRQAYIDYLVDL
                     YSADLIQTLKDNAEIIKINI"
     gene            1572..>2947
                     /locus_tag="PLW82_05710"
     CDS             1572..>2947
                     /locus_tag="PLW82_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOJ46805.1"
                     /translation="MRKLFRSVLLGMILCLFGVAVLASADAIEAFEGTWTIGEDAIVS
                     QIDDNPEIDFSSAYFVEPRSSFYFETDFTILESKNLSRWFGISYRVSEDKSLSHMIRV
                     KQNATLGNGIELTFLTEEGRWRRQRTVAGDSVLELNRKYNLKVAVSEDYVIALLDDQA
                     LIVTKSPTSIGEGLVGLHTNGMTVKFENTVIRDYVKEEMAYFEEWANWQAGRDRAAAH
                     QTIPIIVAHRGNSSEAPENTMAAVKSAIAVGADGVEVDVYRTLDGELVLLHDGTLERT
                     TNGTGHVNNRTWDYIKTLDAGSWFSKEFKGEPIPRLEDVLLECKDKVLLVIELKQAGI
                     ERDVLRLVEETGMKDQVVIIAFGANLLSNIYYMDPSIPTSVLSYTTTTINDVVALAAQ
                     AKTRSVDLNYGVINKEMATWLISRGYSLWAWTVNNVNDMQRMVDMGVSVITTDRPRDA
                     LNYFRPMN"
BASE COUNT          750 a          715 c          669 g          813 t
ORIGIN      
        1 tacttctcca tcgcggtcta cgaatatctc caaggggtcg ccttcccgaa tgcgcaaagt
       61 tctacgaatt tccttaggta taactactct acctaggtcg tctatcctac gcacaatccc
      121 tgtagctttc acgcgcaatc cctccttcac tcctagtttc tctagaagtg agggagttta
      181 tgaaagaaaa atcatattta tctggccttg ggtttttctc agatgttaat cttaatgatc
      241 tcggcgttat ccttcaaagt ttgaatcagg tccgcgctgt agaggtccac cagatagtcg
      301 atgtaggcct gtctgacctg gtctttaatc tcgctgtaat ctgggtttga tgctgggcgg
      361 atatcctcta catacactag atgccaaccg tattcgcttc tcatcggtgc cgagaaggtt
      421 cccttaccga gctggaacgc ggtttcggtt acgcccagcc actcatcatc gcgggtgaga
      481 tagccgatta agccgccttc cttgttcgta ctaggatcaa gggaataccg ttcggcaact
      541 gtaccaaaat cctcgcctct gagaagatcc gcataggctt tcttcgcttc ggactcatcg
      601 atggtgaaaa tgtggcgcac taaacggaac tcaggcgtct ggaaaagatc tttgttgttt
      661 tcgtaatagg caacaacttc ggtttcggga atgtcgctga tgtaaaagag aatgatcttt
      721 tcaatggtga ggttgtgtct aatgctgtct ctaagatcag cttcggagat gctgtaatct
      781 gtgaggaatt tcctgtagag gtcctctcct ccgagggagt ctttgatctc ctgaagtctt
      841 ctggtgacat cgttttcagt aacctgaact ccgagtttct tggcctcctg ctcaatgagg
      901 acctgctcga cgatggtgtt gagctgtgct tccctgtttc tggtcaaaag acggtcgagc
      961 aaatcgtctt tagtaatctt atcctgaccg acaactgcca cgacttcagg ttgggactcc
     1021 tcccaccaca ggtagccgag gacgatgttg cctgctacag agatgactaa taaggtgact
     1081 aaaatagcaa ttactacttt tctggacata gctactctcc cttcccacgc tttctctctt
     1141 cgtttaagtt cgactcgtgg acctttaata cctttggtgc ttgctttgct aattcttctc
     1201 taagccttgt attttaccta gcttcggaaa aacctactaa ttttttcgat ttatctttta
     1261 ccgagcgtat acctggcgtc taaaaccaca gactaaagat ttagactgac agtacggtgg
     1321 tttactgcat tccaccttgc aacttacgcc agtgcccata gcaatcctcc atgaaaacag
     1381 tgtttagtaa aacttcatag atagtgcttc ttttttgtcg ctaacaacct ccagagagtt
     1441 cgaaatcatc ccgttctttt tccacaatac ttttgcctat ctttatgctg agcaagcagg
     1501 tttttacaac tttttgctga aaatttaacg tgcgaaacag atttccaatt ggtaatgaga
     1561 ggagagataa catgaggaaa ctttttcgtt ccgtactgct gggcatgata ctctgtcttt
     1621 ttggtgtcgc tgttctggca tctgcagatg ccattgaggc atttgaagga acttggacca
     1681 tcggagaaga tgcgatcgtc tcgcaaatcg acgacaaccc cgagatcgat ttcagttccg
     1741 cttatttcgt agagccaaga agcagcttct actttgaaac cgatttcacg attttggaaa
     1801 gcaagaatct cagcagatgg tttggtattt cctaccgcgt aagcgaagac aaatcccttt
     1861 cgcacatgat tcgtgtcaag cagaacgcta cactgggcaa cggtattgag ctcaccttcc
     1921 taacagaaga aggaagatgg agaagacagc gtaccgtcgc tggcgacagc gttctggagc
     1981 tgaaccggaa atacaatctg aaagttgctg tatctgaaga ttatgtcatc gccctacttg
     2041 acgatcaagc gcttatcgta accaagagcc ccaccagcat tggtgaaggt cttgtcggtc
     2101 tgcacacaaa cggcatgacc gtgaagtttg aaaacactgt cattcgcgac tatgtcaaag
     2161 aagagatggc ctacttcgaa gagtgggcaa actggcaagc aggccgcgac cgcgctgcag
     2221 cccatcaaac aattccaatt atcgtggcac atagaggcaa ctcctctgag gcaccagaaa
     2281 acaccatggc ggcagttaag tcggccattg ccgttggagc agacggtgtt gaagtcgacg
     2341 tttatcgcac cttagacggt gagctggtcc tcctccacga cggaacctta gaacgcacaa
     2401 ctaatggcac tggccacgta aacaacagaa cttgggacta catcaagact cttgatgccg
     2461 gttcctggtt ctcgaaagag ttcaagggcg aacctattcc tcgcttggaa gacgtactcc
     2521 tcgagtgcaa ggataaggtc ctcctcgtta tcgaacttaa gcaagcaggc atcgaaagag
     2581 atgtcttgag gttggttgag gaaacgggaa tgaaggatca ggtagtgatt atcgctttcg
     2641 gtgctaacct cctgtccaac atctactata tggatccgag cattccaaca tctgttcttt
     2701 cctacaccac gactaccatt aatgatgtag ttgctttagc agctcaagct aaaacgcgtt
     2761 ctgtcgactt gaactacggt gtcattaaca aagaaatggc aacctggttg atcagccgtg
     2821 gttactccct ctgggcttgg actgtgaaca acgtgaacga catgcagaga atggtagaca
     2881 tgggcgtttc ggtcatcaca accgacagac caagagatgc cctgaactac ttcagaccaa
     2941 tgaacta
//
LOCUS       DAOSDA010000221         2937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21776_length_2937_cov_3.526718, whole genome shotgun sequence.
ACCESSION   DAOSDA010000221 DAOSDA010000000
VERSION     DAOSDA010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21776_length_2937_cov_3.526718"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..130
                     /locus_tag="PLW82_05715"
     tRNA            56..130
                     /locus_tag="PLW82_05715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89..91,aa:Arg,seq:ccg)
     gene            263..880
                     /gene="yyaC"
                     /locus_tag="PLW82_05720"
     CDS             263..880
                     /gene="yyaC"
                     /locus_tag="PLW82_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HOJ46806.1"
                     /translation="MWFFGGQETQRFRVHYKDPAAQEKLTSQLTKFLRDKFLPTGKRL
                     AIVCVGTDRSTGDSLGPLTGTLLSRDFPGIPVFGTLDDPIHAANLSEKVAEINSKLKN
                     PFIIALDACLGQSESVGYISCKEGALKPGTGVNKELPEVGNMNVVGIVNVGGFMEYFV
                     LQNTRLSLVMQMAEILAASIGEAVSNLSLTVDAGDLPAAASKWLD"
     gene            complement(956..1396)
                     /locus_tag="PLW82_05725"
     CDS             complement(956..1396)
                     /locus_tag="PLW82_05725"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOJ46807.1"
                     /translation="MFGKKRPEGNNMQDAKRRTQVVKAVDTIIGKTTELTGTIKTSGS
                     VRIDGKFKGEIFTTADVVIGDQGEVTAEIHAVNLTIAGKVEGNVEVKGRLEIVPTGTL
                     LGNATVGVLVIEEGARLEGQIEMINKEGQRVIKQAEAKKEAVAN"
     gene            complement(1410..2522)
                     /locus_tag="PLW82_05730"
     CDS             complement(1410..2522)
                     /locus_tag="PLW82_05730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ46808.1"
                     /translation="MFWLISRFLEFYRTNKGKKITFMSVPDDHGEVKTLAVPVLGLYF
                     GIAAVIVIAFSTIMVVWNYRDVRNNYATVFDLLQKKNTQYEAVLDTSQEQKKTVESLI
                     TETEELKLKLKQLENAFTDINRLLEQTGNRPVQIKPLPERQDTVVASTTTTPVVSQAG
                     IGLPLENRLGLDDKKNLMIFTGVAFAASNVQDIKPPVDQQEVENLLLDLQERLSSIEQ
                     TLVQLEKVSMNTKNRLIQLKYEQDHTPDIWPAKAYISSAYGWRTHPIYGDRRKHTGID
                     IGVPKGTPVKATADGVVSRAGWVSGYGILVQIDHGNGIETLYGHLSRAVVKKGDTVKK
                     GQLIAYSGNTGLSTGDHLHYEVRLNGKDVNPMNYLP"
BASE COUNT          697 a          718 c          622 g          900 t
ORIGIN      
        1 cgcggtgcct cccttgttac gttccgattc ctgtgctata atttaataac aatgtgcggc
       61 tatagctcag gggatagagc accggcctcc ggagccggtt gcccaggttc gattcctggt
      121 agccgcacca ttattatgcc tatgacctgt atgataattt aacttattgt acaggttttt
      181 atttttttag gaatattttt gtgacaaata catatactga ctgtgagtga tttatccatc
      241 ccaactagag aggaagattt acatgtggtt ctttggggga caagaaaccc agcgctttcg
      301 ggtacattac aaagacccag cagcccagga aaagctcaca agtcagctga cgaagttttt
      361 acgcgacaaa ttcctcccta ctggaaaaag gctagcgata gtatgtgtcg gcacagatcg
      421 ttctacagga gattccttgg ggcctctaac tggaacgctg ttgtctagag atttcccggg
      481 aattccggtt tttgggacgc tcgatgaccc tatccacgca gcaaaccttt ccgaaaaagt
      541 ggcggaaatc aactcgaaac taaaaaaccc cttcatcatc gctttggacg catgtttagg
      601 tcagtcagaa agcgtcggtt atataagctg caaagaagga gctctaaaac ccggaacggg
      661 agtcaataag gagctaccgg aagttggcaa catgaacgtt gttggtatcg tcaatgttgg
      721 agggttcatg gagtattttg ttctgcagaa taccagactc agccttgtga tgcagatggc
      781 agaaattctc gctgcctcta ttggcgaagc tgtatcgaac ttgtccttga ccgtagatgc
      841 aggagacctt cctgctgcag cgtcaaaatg gcttgattag atctacctga gttcacctct
      901 tgttacttaa caaaagaaac tgaggccaca cgcctcagtt tcttgatttt tgagtttaat
      961 tcgcaaccgc ttcttttttt gcttctgcct gtttgatcac tctttggcct tctttattga
     1021 tcatttcgat ctggccttcc agtcgggcgc cttcttctat aacgagtaca ccgactgttg
     1081 cattgccaag aagcgttccc gttggaacta tctccaacct accctttacc tcaacattgc
     1141 cttctacctt ccctgctatg gtgaggttga ccgcatgaat ttcggccgtt acttcacctt
     1201 ggtcgccgat tacaacatca gcagtagtga agatctcgcc cttaaactta ccatcaatgc
     1261 ggacggaacc tgaagttttg atcgtaccag tgagttcagt ggtctttccg attatggtat
     1321 cgacagcttt caccacttgc gttcttcttt tggcatcctg catgttattg ccctccggtc
     1381 tcttttttcc aaacatttat tcacaactcc tatggcaagt aattcattgg gttcacgtct
     1441 ttgccattaa gccgaacctc ataatggagg tgatcgcctg tcgagagccc cgtgttaccg
     1501 gaatacgcga tcagctgtcc tttcttaact gtatcacctt ttttaaccac cgctctggag
     1561 aggtgtccgt atagggtttc aatgccatta ccgtggtcga tctgcaccag tatgccgtat
     1621 ccactgaccc atccagctct ggaaacgacg ccgtcagcgg tagctttgac tggggttcct
     1681 tttggaacac caatatctat acctgtgtgc ttacgccgat ccccatagat agggtgggtt
     1741 ctccagccgt atgctgaaga gatgtaggct ttcgccggcc aaatatccgg cgtatgatcc
     1801 tgctcatact tcaattgtat gagtcggttc ttcgtgttca tgcttacttt ttccagctgc
     1861 acaagagttt gctcgattga agataaccgt tcttgcaagt ctaaaagtaa gttctccacc
     1921 tcttgctgat caacaggtgg ttttatatcc tgaacgttgg acgctgcaaa cgccacacct
     1981 gtaaagatca ttagattctt tttgtcatcc aatcccaaac ggttctcaag gggaagacca
     2041 atacctgctt gagacacgac gggagtagtt gttgtagaag caacgacagt atcctgtctt
     2101 tcgggaagag gcttgatctg tactggtcga tttcctgtct gttctaaaag tctgtttata
     2161 tcggtaaagg cgttctccag ctgctttagt ttcaacttta gttcttcggt ttccgtgatc
     2221 aatgattcta ccgttttctt ttgctcttga gatgtatcca ggaccgcttc atactgcgtg
     2281 tttttcttct gcagcagatc gaagactgta gcatagttgt tccttacgtc acggtagttc
     2341 catacgacca ttatggtgga gaaagctatc acgataactg cggctattcc aaagtacaat
     2401 cccaaaactg gaaccgctaa agttttaacc tctccatgat cgtctggaac tgacatgaag
     2461 gtaatcttct tgcccttgtt tgtccgataa aactctaaaa aacgagagat caaccagaac
     2521 attctcttcc ctcctttctt gttgtcgtct ttgttgcatc taggaaacgt ggtctaatta
     2581 taccaaattt ctacttctct tttaaccctt tatttcctgc ccgtcgcgtt ttttgagact
     2641 aacgccctaa tcctaaaagg gcctctagtc ccgaaccgct ctgagaggaa gtttttctat
     2701 tgttaaataa ttaacgaaga ttaggtccat ggacttttcc cagcggattg gtcgccagaa
     2761 ctttcgagct ggatgcacac taccccgact tgcgttggca accgacagct tcagacgtgt
     2821 actgtagggt ctatcatgaa cagcccctct gcaatccaaa tatggcaccc gctgcacggc
     2881 agtccttgta cactctttat catcattgtt aatattattt tccatttttc cttctta
//
LOCUS       DAOSDA010000222         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21813_length_2934_cov_2.713790, whole genome shotgun sequence.
ACCESSION   DAOSDA010000222 DAOSDA010000000
VERSION     DAOSDA010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21813_length_2934_cov_2.713790"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLW82_05735"
     CDS             <1..293
                     /locus_tag="PLW82_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOJ46809.1"
                     /translation="SGPKMHISRYRSQKESIKTEINVIGKTVEEAVDEIDQYLDSAFL
                     AGLGSVRIVHGKGTGALRKGIQEYLKAHPHVKGFRDGAQGEGGIGATVVELN"
     gene            complement(481..1968)
                     /locus_tag="PLW82_05740"
     CDS             complement(481..1968)
                     /locus_tag="PLW82_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ46810.1"
                     /translation="MKRIYILLLVLAVSLSCLADKYLIYSGDALLGTEEVTVFDNKII
                     TKTTLNASGVQLSYTQTLTLSSGKPSQYQANINGVVNITGSFADGEATYTVANQKRTF
                     KGNGLYPVENNTFYLWSYVLKNAPQDLVVPSQFASVKAVYSSWQTGPYQTQVRQVNIQ
                     QLALIGYFSDGKMVRFTIPLQNIDVIHEDWVEIFRESSGQTVISETVVLKRGSDLLHG
                     ELNLPANKTNYPVLLLISGSGPQDRDSNSPPEMTNSLFKHLSITLTNLGIGTLRFDDR
                     GTGKSTGNHMAQDYETLIQDAREALDYLRNRSDVSKIGILGHSEGGLTALILGAEEED
                     IAFLVLMAAPSTSLDKIIIEQVHAQKELPGITAEDVAYLDQILAIVESSIELAKSGAE
                     ATPLGFTGKYLRDHLEIDPLEYAEKITCPVLILQGDADVKILPYHAKKLENALANAQV
                     ITYPYLHHYFTPSSLNNPEFTLESAYKTPDKVYNDIAAFLLEHAF"
     gene            2036..2518
                     /locus_tag="PLW82_05745"
     CDS             2036..2518
                     /locus_tag="PLW82_05745"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOJ46811.1"
                     /translation="MFYIDSFDLDESLSCHLVYTEKGLVYLGLNEYDPPKFVPEISAL
                     VPLPFFRLRRDLLRYLEGEKVDFRYELDLRTTPFRKKVYRQLCLIPYGSVLTYKEIGE
                     NISLPNGQRAVGQAVGSNPLPIIVPCHRVVAQSGLGGFSLGLEMKRKLLAIENSLGAF
                     "
     gene            2637..>2934
                     /locus_tag="PLW82_05750"
     CDS             2637..>2934
                     /locus_tag="PLW82_05750"
                     /inference="COORDINATES: protein motif:HMM:NF028409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HOJ46812.1"
                     /translation="MRTNAKTRDLALAGLMIAAGVVLHSFPPIIGGMKPDFSLIMLIL
                     YALLRRDKKVTLAAGLATGVITAITTGFPGGQIPNVIDKTLTTLMLLGITALPES"
BASE COUNT          719 a          686 c          770 g          759 t
ORIGIN      
        1 actccggccc gaagatgcac atctcccgat acaggtcgca aaaagagtcc atcaaaaccg
       61 agattaatgt tatcggcaag acggtagaag aagccgtcga tgagatagat caatacttgg
      121 actcggcgtt tttggccggt cttggttcgg tacgtatagt gcacggaaaa ggtaccgggg
      181 ctttgcgtaa agggatccag gagtacctaa aagctcatcc tcatgtaaag ggctttagag
      241 acggcgctca aggtgaaggc gggattggag ctacagtagt ggagctgaat taacttcaga
      301 cgaaagactc gaatacccaa ttcgcctttt tgaccgatcc tgcgatcaca gacgcaagaa
      361 aggcagctta aaccgcaaaa gcggtactta taggttagat gcgtaaagaa aacgtccacc
      421 aacgccattt cggcgaaggg tggacgtttg tctatagctg ttccaagtct tgagaacctc
      481 ttagaaagcg tgttccaaga gaaaagctgc aatgtcgttg tacactttat ctggtgtttt
      541 gtatgctgat tccaatgtga actcggggtt gtttaaagat gacggcgtga agtagtggtg
      601 gaggtagggg tacgtaatta cctgagcatt tgccagagca ttctccagtt tttttgcgtg
      661 gtagggcagg attttaacat cagcatcgcc ctgaaggatc aaaacgggac aggtgatttt
      721 ttctgcatac tcaaggggat cgatctccag atgatccctg aggtacttgc ctgtgaagcc
      781 tagtggcgtt gcctctgcac cgcttttagc aagctctata ctgctttcaa caatagctag
      841 gatctggtct agataagcga cgtcttctgc agttatgcct ggaagctctt tttgggcgtg
      901 tacctgttca atgatgatct tgtctaaaga agtgcttggt gcagccatga gtactaaaaa
      961 agcaatgtcc tcttcctcag cccccaagat gagggcggta aggcctcctt cgctgtggcc
     1021 taaaatgccg atcttggaaa cgtcggacct gtttctcaag tagtccaggg cttcccttgc
     1081 atcttggatt aaagtctcgt agtcctgagc catgtggttt ccagtacttt tgccggttcc
     1141 ccgatcatcg aaacggagag taccgattcc gagattcgtc agggtgatgc tcaagtgctt
     1201 gaacaagctg tttgtcatct caggaggtga gttgctgtcc cgatcctgag gacctgaccc
     1261 cgagattaag agaagtactg ggtaattggt cttatttgcc ggcaaattga gttcgccatg
     1321 taggagatcg cttccccgct tgagaactac tgtttcagag attacagtct ggccgctgga
     1381 ttctctgaag atttccaccc agtcctcatg gatcacatcg atgttctgga gcggaatggt
     1441 gaacctgacc attttgccgt ctgagaaata gccgatcaga gcaagctgct ggatgttcac
     1501 ctgccgcacc tgagtctggt acggtccggt ctgccaagag ctgtagaccg cttttaccga
     1561 ggcaaactgc gaaggcacta ccaaatcctg tggggcgttt tttagtacat aagaccacag
     1621 gtagaaggtg ttgttttcca caggatacaa cccattaccc ttaaaggttc gcttctgatt
     1681 tgctacggta tatgtagctt cgccgtctgc aaagctaccg gtgatgttta ccactccgtt
     1741 gatgttcgcc tgatattggc tgggttttcc acttgataga gtgagggttt gagtgtaact
     1801 gagctggacg cctgatgcgt tcagcgtggt tttggtgatg atcttgttgt caaagactgt
     1861 tacctcttcg gttcccagaa gggcgtcgcc tgaatagatc aagtacttgt cggccaaaca
     1921 actcaacgat actgccaaaa ccaaaagcaa aatgtagatg cgcttcaagt gtagacaact
     1981 cctttcacta atctatacgc aaagaccggt caaaaagttt cgagagggga ttgcaatgtt
     2041 ttatatagac agctttgatc tcgatgaaag tttgtcgtgc cacttggttt acacagaaaa
     2101 aggcctggtc tatttggggc tgaacgaata cgatccgccg aagttcgtgc ccgagatctc
     2161 cgcgctggtg cctctgccct ttttccgctt gcgccgcgac cttctgcggt atctcgaagg
     2221 cgaaaaagtc gacttccgct atgagttgga tctgcgcacc acacccttca gaaagaaggt
     2281 gtaccgccag ctctgcctca ttccttacgg cagcgtgctc acctacaagg agattggcga
     2341 gaacatcagt ctccccaatg gtcagcgggc agtgggtcag gctgtaggca gcaatcccct
     2401 acctatcatc gttccctgcc accgggttgt agcgcaatcc ggtttgggcg gctttagtct
     2461 gggactggag atgaaaagaa agcttttagc catcgagaac tccctaggag cgttctgagg
     2521 gaaaacccga gaagcaaaat ttactattga cggtggaaac tgtgctgtga tatccttaga
     2581 gaaagtttag cttagctgag caaagactag aatgagaaga ggagagatga gttgagatga
     2641 gaacaaatgc aaagacacgt gatttggcac ttgctgggct gatgatcgcc gcaggagttg
     2701 tgctgcattc ttttccgccg attatcgggg gaatgaagcc ggacttctcc ctgatcatgc
     2761 ttattctgta cgccctgctg cgccgagaca agaaggtcac gctggctgcc ggactggcca
     2821 caggcgttat caccgccata accaccggtt ttcccggcgg acagattccc aatgttattg
     2881 acaagaccct gacaaccctg atgctgctcg ggattacggc gcttccagaa agct
//
LOCUS       DAOSDA010000223         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21820_length_2933_cov_3.025365, whole genome shotgun sequence.
ACCESSION   DAOSDA010000223 DAOSDA010000000
VERSION     DAOSDA010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21820_length_2933_cov_3.025365"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..682)
                     /locus_tag="PLW82_05755"
     CDS             complement(74..682)
                     /locus_tag="PLW82_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46813.1"
                     /translation="MQKRRFLIALCILLVLSAAAFAEKITLWTWYDGSLGVIFRKLVQ
                     EEFVQKTGIEVEILTVPIADIVNKLLLAYIGGDAPDVVELYTNQVVDLGVRGALMDLT
                     QFPDYQEVVSGLYPNYLKQLQYKSATFALPCEIAWPWTYVRTDIFNDFGLEIPNTWDE
                     FATSITKLKARGLGTFYTHTGDAATITASRFLAFVYQRKTDI"
     gene            complement(761..>2933)
                     /locus_tag="PLW82_05760"
     CDS             complement(761..>2933)
                     /locus_tag="PLW82_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ46814.1"
                     /translation="IPFRIGFRTVEIKDGNLLVNGTPIMIKGVNRHEFHPVLGRTLSY
                     EGQKRDLLIMKKHNINAVRTAHYPNHPVFYELCDELGIYVVDEADLECHGVELAGDAD
                     LLASDPRFQKAFVDRIERTIHRDRNHPSVIIWSMGNESGFGPNIVAMAETARRLDPTR
                     PIHYERDNSVSMLASDIICPMYVSLEQVINLGESRTAYHGPMVFDPERLKPYPVILCE
                     YAHAMGNGPGGLKDYWDAFYKYPKLQGGFLWDFVDQGLLQQREGKEFYAYGGDFGDKL
                     NDGNFCLNGLVFPDRSSSPALIEYKKAIEPVKVLEVNLEAGSILIENRYDFLTLDHIL
                     LTWEVKSYGRSLFSGSQELTGIAPRQKMWIDIAGFKKIHWETLPESTLNFSFILREPE
                     SFAPCGHEVAFHQEKLKSARTKHQKIASEVPFSVKACEDPEKIVFTDGGNRIIFSKIT
                     GTLESWTFCGTDVIKKGPKINLWRAPIDNDRPFVESWQKHKLDQLQLQPRSVHFDPHS
                     HLIQVNGKVIAPSLRWFIDVELTYQLQGGVLRMSVHGEPSGGPEIWPRIGLELQLAKE
                     LSTFTYYGKGPHESYPDSSASARLDLYTQEISDLFTNYIYPQENGNRSEVEFLFAGSP
                     RGAGLFVAGSEPFNFSARRFSLENLTCAKHTYDLNEDNATYLYLDQEVLGLGSASCGP
                     TPTEQYLVRNKPFSFTFYFTPLSSSSLPLHHLASQIKAKLD"
BASE COUNT          678 a          704 c          780 g          771 t
ORIGIN      
        1 acagcgaggt gaacttcatc caggcatcta tcacttctgg ggcatccagg ttcgaagcgg
       61 taccatcagg agtttagata tcggtttttc tctggtagac gaaagccagg aacctgctgg
      121 cggtaattgt agcagcatct ccggtgtggg tgtagaaggt gccaaggcca cgggctttaa
      181 gtttggtgat ggaggtggca aactcgtccc aggtgtttgg gatctcgagg ccgaagtcat
      241 tgaagatgtc tgttcttaca taggtccaag gccaagcgat ctcgcacgga agggcaaagg
      301 ttgcgctttt gtactgcaac tgcttgaggt agttggggta cagaccgctt accacctctt
      361 ggtaatcggg gaactgggtc aggtccatta aggcgcctct aacgcctaga tctaccacct
      421 gattggtgta cagttccaca acgtctgggg catcaccgcc gatgtaggca aggagaagtt
      481 tgttcacaat atctgcgatg ggcaccgtca ggatctccac ttcgatgccg gttttctgaa
      541 caaactcttc ctgcaccagc ttgcggaaga tgacgccaag gctgccgtca taccaagtcc
      601 acagagtgat cttctctgca aaagcggcag cgcttagaac aagcaggatg caaagagcaa
      661 tcaagaacct tcttttttgc atgttctaac ctccactttc tgcgatcttt ttaatcagac
      721 gcctataggc tctgtatatg cctttggggc gtaaggtaga tcagtccagc tttgctttga
      781 tttggctagc taagtgatgg agaggtagac tgctgcttga tagaggtgtg aagtagaagg
      841 taaagctaaa gggtttattg cggacgagat actgttctgt gggagtcggt ccgcagcttg
      901 cagagccaag tcccaaaacc tcctggtcca gataaaggta ggtagcattg tcctcattta
      961 aatcataggt atgcttagcg caggtgaggt tttccaagga aaagcgcctg gcgctgaagt
     1021 tgaatggttc gcttccagca acaaacagtc cagcacctct ggggctacct gcaaaaagaa
     1081 attctacttc cgacctgttg ccgttttcct ggggatagat gtagtttgtg aacagatcgg
     1141 agatctcctg ggtatagagg tccagccttg ccgaagcgga gctatccgga tagctttcat
     1201 ggggtccttt gccgtaatag gtaaaggtgg aaagctcttt tgccagttga agttcgagtc
     1261 cgattctcgg ccagatctcc ggaccaccgc tgggctctcc gtgcaccgac atccgcaaaa
     1321 cgccaccctg cagctggtag gttagttcaa catcgatgaa ccacctgaga gatggagcta
     1381 taaccttgcc attgacctgt atcaggtgag agtgcgggtc gaagtgcacc gatctgggct
     1441 ggagttgcaa ttggtctagc ttgtgctttt gccacgactc cacaaaaggc ctgtcgttgt
     1501 ctataggagc tctccaaaga ttgatcttcg ggcctttctt aattacatcg gtaccacaaa
     1561 acgtccagct ttcaagagtg ccggtaatct tgctgaatat gatgcggttt ccgccatctg
     1621 taaagacaat tttctctgga tcttcacagg ctttgacgga gaacgggact tcgcttgcga
     1681 tcttttggtg tttcgttctt gcagatttaa gcttttcctg gtggaatgct acctcgtgtc
     1741 cacaaggagc aaaggattcc ggctccctca ggataaacga gaagttcaaa gtactctcgg
     1801 gcagagtctc ccaatggatc ttcttaaacc ctgcgatgtc gatccacatc ttttgccgtg
     1861 gcgcaatgcc cgtcagttcc tgggagccgg agaaaaggga tctgccatag cttttgacct
     1921 cccaggtaag gaggatgtgg tccagggtaa gaaaatcgta gcggttttcg atgaggatgc
     1981 ttcctgcctc caggttgact tccagcacct tcacgggctc gatggctttt ttgtactcaa
     2041 tgagagcagg tgaactgctc cggtcgggga aaaccagacc attgaggcag aagttcccat
     2101 cgttgagttt atcgccaaag tctccaccgt aggcatagaa ctctttgccc tctctttgct
     2161 gcagaagtcc ctggtctaca aagtcccaga gaaatcctcc ctgcagcttg ggatacttat
     2221 agaaggcatc ccagtagtct ttgagccctc cggggccgtt tcccatagca tgggcgtact
     2281 cgcataagat cacaggatag ggctttagcc tttccggatc gaaaaccatc ggaccgtggt
     2341 aagcggtcct gctttcacct aagttgatta cctgttccag agaaacgtac atggggcaga
     2401 tgatatctga tgccagcatg gagacgctgt tgtctctttc atagtggata ggccttgtgg
     2461 ggtcgagccg tcttgcagtt tctgccatag cgacaatgtt cggtccgaac cccgactcgt
     2521 tgcccataga ccagataata accgaaggat ggtttctgtc tcggtgaatg gttctttcga
     2581 tcctatccac aaaagccttt tggaagcgag gatcgctggc caaaaggtcc gcatctcctg
     2641 ctagttctac gccgtggcat tcaagatctg cttcatcaac aacgtagatg ccaagttcgt
     2701 cgcagagttc gtagaacaca gggtgattgg gatagtgagc ggttcggacc gcattgatgt
     2761 tgtgcttttt cataatcaga aggtctctct tctggccttc gtaggagaga gttctcccta
     2821 gaactgggtg gaactcgtgc cggttcacac ctttgatcat gatcggagtg ccgtttacaa
     2881 ggaggttgcc gtctttgatc tcaacagtgc gaaaaccgat cctaaatggg atg
//
LOCUS       DAOSDA010000224         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21822_length_2933_cov_2.739402, whole genome shotgun sequence.
ACCESSION   DAOSDA010000224 DAOSDA010000000
VERSION     DAOSDA010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21822_length_2933_cov_2.739402"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLW82_05765"
     CDS             <1..202
                     /locus_tag="PLW82_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46815.1"
                     /translation="MKGEKIDQDLKELEELWGVISLNPYVRAVVENELIFKEFYSQII
                     RELSKGLELEEQSETKEDENSK"
     gene            199..681
                     /locus_tag="PLW82_05770"
     CDS             199..681
                     /locus_tag="PLW82_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOJ46816.1"
                     /translation="MIIGLNVRKAESAVVVGDVVLSDNVSIWYNAVVRGDIAPIRIGK
                     DSNVQENAVIHVSRDIPVSIGERVTIGHGAIIHSCTIEDDVLIGMGATILDGAVVGQG
                     AMIGAGALVGPGKVIPPNSLVVGIPGKVVRELTPEELEANRENIREYLEISSSLPEFK
                     "
     gene            complement(801..1460)
                     /locus_tag="PLW82_05775"
     CDS             complement(801..1460)
                     /locus_tag="PLW82_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46817.1"
                     /translation="MEFFTEYLEYLEVPYEISGNLIKTKIPHGEGTLEAEYCFSADDH
                     RPGVLGFFQGTDLFYNVLEGCLKRGAWVTAYLTKNRFVIPEAPFVPYLFLNLHAVSPA
                     ACAPRKNYSLLINLCTGDLLDDPPLKSYRQIAKDRSLPRALSYKAALQKGLEYIKKDL
                     ASQTGWLEESKKNRESFLANLKRAGLTEAEERTRSGEARARLSPHFEVHVRLLGRLFI
                     K"
     gene            complement(1469..>2933)
                     /locus_tag="PLW82_05780"
     CDS             complement(1469..>2933)
                     /locus_tag="PLW82_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOJ46818.1"
                     /translation="GQSGLLSLEKLKEYLRLVGLVPYSHQVECVRRVVDEMNGQAILA
                     DEVGLGKTIEAGLILREYLERGLLKKALILTPASLTRQWEWELRQKLGLRVYRQRTIY
                     DWQNSHILIASLDTAKKEPHRSLVLEVDWDLVIVDEAHHLKNHKTKAYKFVSELKKRH
                     LLLVTATPMQNNLRELFNLVQLIAPGSLGSLQDFSRRYGLPDSASLNAIRDNLRPFLI
                     RTTKKEAMLELPPRNVEHHGCMQDEAENLLYTALSEYISTGLRAEKGKSLTLITLQKE
                     LCSSVFAALLTLEKLLNETADPIVGKLVELGTIVKMNAKTRYLLELLPQIEGKVVVFT
                     EYLATQRFLAGELKKRRISFVTFDGTLSSSKKEFAKERFRRAAKVLLATESGGEGLNL
                     QFCQNVINYDLPWNPMKLEQRIGRLHRLGQEKPVNVLNLFTADTIEEHILKLLYQKLR
                     LFADVFSFDDECLGEDLTKYIVAEILGLDVGKERAFS"
BASE COUNT          688 a          706 c          799 g          740 t
ORIGIN      
        1 catgaaaggc gaaaagatcg atcaggatct aaaggaactg gaagagctct ggggggtcat
       61 cagcctcaac ccgtatgtga gggctgtagt tgaaaacgag ctgatcttca aggagttcta
      121 cagccagatc attcgcgagc tcagcaaagg attagagctc gaagaacaaa gtgaaacaaa
      181 agaggatgaa aacagtaaat gataattggt ttgaacgtaa gaaaggcgga aagcgcagta
      241 gttgttggcg acgtggtgtt aagcgataac gtcagcattt ggtacaacgc cgtggtcaga
      301 ggcgatattg cgccgatccg tatcggtaaa gacagtaatg ttcaggagaa cgcagtaatt
      361 cacgtatcaa gagacattcc cgtttccatt ggtgaaagag tcaccatcgg acacggagct
      421 atcatccaca gctgcaccat cgaagatgat gttctcatcg gtatgggtgc caccatcctg
      481 gatggggctg tcgtcgggca gggagctatg atcggtgcag gtgcactagt tggacctggc
      541 aaagtcatcc ctccgaactc tctggttgtc ggcattccag gtaaagtagt ccgcgaactt
      601 actccagaag agcttgaggc aaaccgggag aacattcgcg agtatcttga gatatcgagc
      661 agcctgcccg aatttaagta agcggaacct aaagcaaaac tcatgatagc gttaaagcgg
      721 cacttaacga aagtgccgct ttttatgcct ccgagcccag atcgatcaag atgggctaga
      781 tgaggtctcg ggtttttttt ctacttgata aagagcctgc caagaagtcg gacatgaact
      841 tcaaaatggg gagacagccg cgctctagct tcgccgctcc gggtcctttc ttcagcttct
      901 gtaagacctg cccgcttgag attggcaaga aagctttcgc ggtttttttt gctttcttca
      961 agccagcctg tctgggaggc aagatccttc ttgatgtact ctaacccctt ctggagtgcc
     1021 gctttgtatg acaaagccct cggcaaagac cggtcctttg cgatttgccg gtagcttttc
     1081 agcggtggat cgtctaacag gtccccagtg cagaggttga tcaagagcga gtagtttttt
     1141 cgcggggcac aagccgcagg agaaaccgcg tgtaggttca ggaagaggta ggggacaaac
     1201 ggggcttccg ggatcacaaa gcggtttttc gtgaggtagg ccgtgaccca ggcacccctt
     1261 ttgaggcagc cttcgagcac gttgtagaaa aggtcggttc cctggaaaaa gcccagcact
     1321 cccggccgat ggtcatctgc ggaaaagcag tattcagctt cgagggtgcc ttcgccatgg
     1381 ggaattttgg tcttgatcag atttcctgaa atctcgtagg gaacttccag gtattctagg
     1441 tactctgtga aaaactccat ccaatctgtc aagaaaacgc ccgctccttc cctacatcca
     1501 gacctagaat ctcggcgaca atgtacttgg tgagatcttc tcccaggcac tcgtcgtcaa
     1561 atgaaaacac gtcggcgaaa aggcggagct tttgatagag gagtttcaga atgtgctcct
     1621 caatggtatc tgctgtaaag aggttgagta catttaccgg tttctcctga cctaagcggt
     1681 ggagcctgcc gattctctgc tcgagcttca tgggattcca gggaagatcg tagttgatta
     1741 cgttttggca gaactgaagg tttaagcctt ccccgccgga ctcagttgcc agaagcacct
     1801 ttgcagccct gcgaaagcgc tctttggcaa actctttctt agacgaagac agcgttccgt
     1861 cgaaggtgac aaagctgatt ctccgttttt tcaactctcc agcgagaaac ctttgggttg
     1921 ccaggtattc ggtgaaaacc acaaccttac cttcgatctg tgggagcagc tcgagaaggt
     1981 atctggtctt ggcgttcatc ttgacgatcg tccctagctc cacaagtttc ccgacgatgg
     2041 ggtctgcagt ttcgttaagg agcttttcca gggtaaggag agctgcaaag acgctagaac
     2101 agagctcctt ttgcagggtg ataagggtga gacttttacc cttctcggca cggagcccgg
     2161 tggagatgta ctctgacagt gccgtgtaga ggagattttc cgcttcatcc tgcatacagc
     2221 cgtggtgttc tacgtttcgg ggcgggagtt ccaacattgc ttctttctta gtggttctga
     2281 tcaaaaacgg ccgcaggttg tctctgatgg cattcagcga cgccgagtcg ggaagaccgt
     2341 accttcggga gaagtcctgc agagaaccga ggcttcccgg tgctatgagc tgcaccaagt
     2401 tgaacagctc cctgagattg ttctgcatgg gggttgcggt gaccagaaga aggtgcctct
     2461 tctttagctc cgaaacgaac ttgtaggctt tggttttgtg gtttttcagg tggtgggcct
     2521 catcgacgat taccaaatcc caatcgacct ctaaaacgag gctgcggtgg ggttcttttt
     2581 ttgccgtgtc gaggctggcg atgaggatat ggctgttctg ccagtcgtag atggtcctct
     2641 gcctgtagac tcttagtcct agcttttgcc ggagttccca ttcccactgc ctggtgaggg
     2701 aagccggggt gaggatcagg gcttttttca aaagccccct ttctaggtat tctctaagta
     2761 tcaggcctgc ttcgatggtt ttccccaatc ctacttcatc tgctagaatc gcctgcccgt
     2821 tcatctcatc cacaaccctg cgtacgcatt caacttggtg ggagtagggg acaagaccaa
     2881 ctaggcgcag gtactctttg agcttttcta gcgaaaggag tcccgactgc ccc
//
LOCUS       DAOSDA010000225         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21823_length_2933_cov_2.551077, whole genome shotgun sequence.
ACCESSION   DAOSDA010000225 DAOSDA010000000
VERSION     DAOSDA010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21823_length_2933_cov_2.551077"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PLW82_05785"
     CDS             <1..1190
                     /locus_tag="PLW82_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOJ46819.1"
                     /translation="FMDRSVMEGDPHRVIEGRAISAYAIGADEGYIYVRAEYPMAIKR
                     LRIAIQEAIDAGIIGDSVLGSDFSFHVHIKEGAGAFVCGEETALIASIEGKRGMPNPK
                     PPYPTDSGLWGCPTVINNVETLANIPIILSKGPEYFRGVGVGNSPGTKTFALTGQVAN
                     TGLIEVPMGTTLREIVFDIGGGILSGNKYKAVQIGGPSGGCLTEDHLDINLDFDSLQS
                     VGAMIGSGGLVVMDEKTCIVEVARFFMSFTQFESCGKCVLCREGTKRMLEILEDIVGG
                     KGTPEHLELLEELGATISDGSLCGLGKTAPNPVLTTLKYFKDEYLRHVENKVCDAKVC
                     SALKVYTIDPELCKGCTRCKRVCPVGAISGKVKEAHTIDVLTCIKCGACVETCAFNAI
                     KEA"
     gene            1193..>2933
                     /locus_tag="PLW82_05790"
     CDS             1193..>2933
                     /locus_tag="PLW82_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOJ46820.1"
                     /translation="MGNTLYIDGRPVEFTDEKNVLEVVRLAGIELPTFCYHSELSVYG
                     ACRMCVVEVDGMGIVGACSTPPRDGMVVRTNTPRVRNIRRMALELLLANHDRECTTCT
                     KSGQCKLQDLSNRFGIKHVRFGGRDETLALDLSSPSIVRDPNKCILCGDCVRVCREIQ
                     GIGVLDFAHRGSKAQVTPAFGKGLEEVDCVSCGQCAAVCPTAALTIKNETDIAWAALN
                     DPGKVVIAQIAPAVRVAIGEMFGAKPGEINLGKLVSALHLLGFDKVFDTSFTADLTIM
                     EETNEFVERFKKSEKLPQFTSCCPAWVKYAEQFHPELLDNLSSCRSPQGMFGALVKNH
                     LAKEMNIDPKDIFFISIMPCTAKKFEAKRPEFSSGDTPEVDLVLTTQEIGNMIKEAGI
                     DFANLDVEALDMPFGFTSGAGVIFGSSGGVAEASLRAAAEIISGEEIDAVDFKQTRGL
                     TGLKEAEVEISGAKLKIAVVNGLKNAKDLIAKIQSGEVHYDLIEVMACPGGCIGGAGQ
                     PTPNDTTTREQRMKGLYEADKLQQLRKSQQNPIVQKLYSQWLGEPGSDIAHENLHTTY
                     QSRRRIKGQNITLS"
BASE COUNT          748 a          766 c          777 g          642 t
ORIGIN      
        1 catttatgga ccgctctgtt atggagggtg atccccacag agtgatcgaa ggtagggcca
       61 tcagcgctta cgccattggt gccgatgaag gttacatcta cgttcgcgca gagtatccta
      121 tggccatcaa gaggctccgc atagccattc aagaagccat cgatgccggc atcatcggcg
      181 acagcgttct cgggtcggac ttctccttcc acgtgcacat caaagaaggc gcgggagcct
      241 ttgtttgcgg agaagagacg gctttgatcg catcaatcga gggcaagaga ggtatgccca
      301 atcccaaacc gccgtaccct acagacagcg gtctttgggg ctgcccaaca gttatcaaca
      361 acgttgagac tttggcaaac atcccaatca ttctttcaaa gggtcccgag tactttagag
      421 gtgtgggcgt aggaaacagc cccggaacca agacctttgc tcttacaggc caggttgcaa
      481 acaccggtct catcgaagtt cctatgggaa ccactctaag agagatcgtc ttcgatatcg
      541 gcggtggaat cctcagcggg aacaagtaca aagccgtcca gatcggcggt ccttcgggag
      601 gatgcctcac cgaagatcat ctggacatta acctggactt cgactcacta cagtcggttg
      661 gtgcaatgat cggttccggc ggacttgtag tcatggacga gaagacctgt atcgttgagg
      721 tagctcgctt cttcatgagc tttactcagt ttgagtcctg cggtaagtgc gttctctgcc
      781 gcgaaggcac caagcgcatg ctggagatcc tcgaagatat cgtcggcgga aagggaacgc
      841 ctgagcacct tgagcttctc gaagaactcg gcgcaactat cagcgacgga tctctgtgcg
      901 gtctcggcaa aactgcgcca aatcccgtac tcacaacttt gaagtacttc aaagacgagt
      961 acctgcgtca cgtcgagaac aaagtctgcg acgctaaagt ctgctcggcg ctgaaagtct
     1021 acacaatcga tccagaactg tgcaaaggtt gcacaaggtg taaacgtgtt tgccctgttg
     1081 gcgccatttc tggaaaggtc aaggaagcac acactatcga cgtactaaca tgcatcaagt
     1141 gcggtgcttg tgtagaaaca tgcgcattta acgcaataaa ggaggcgtaa taatgggtaa
     1201 caccttgtat atagacggaa gacctgtaga gtttacagat gaaaaaaacg tcctagaagt
     1261 cgttagactt gcaggtatcg aactgcctac tttctgctac cactcggaac tcagcgtata
     1321 cggcgcttgc cgcatgtgcg ttgtggaagt agacggtatg gggatcgtcg gagcgtgttc
     1381 cacaccccca agagacggca tggtggtcag gaccaacact ccaagagtcc gcaacatccg
     1441 cagaatggct ctggagctcc ttctggccaa ccacgacaga gagtgcacca catgcaccaa
     1501 gtcgggtcaa tgcaaactgc aggacctgtc caaccgtttc ggcatcaagc acgtccgttt
     1561 tggcggcagg gacgagaccc tggctttaga tctttcgagc ccgtcgattg tgcgggatcc
     1621 caataagtgc attctctgcg gcgactgcgt cagggtctgt cgtgagattc agggaatcgg
     1681 cgtcttggat tttgcccaca ggggatccaa agctcaggta acgccagctt tcggcaaagg
     1741 cttggaggaa gtcgactgcg tgagctgcgg tcagtgtgca gcagtctgcc cgacagcagc
     1801 tttaaccatc aagaacgaaa cggatattgc ttgggccgcg cttaacgacc ccggtaaagt
     1861 ggtaatcgcc cagatcgcgc ctgcagttag agtcgcgatc ggcgagatgt tcggtgcaaa
     1921 gcccggtgag atcaacctgg gcaagctggt ttcagccttg caccttctag gctttgataa
     1981 agtgttcgac acttcattta ccgcagacct taccatcatg gaagaaacca acgagttcgt
     2041 ggagcgcttc aaaaagtctg aaaagctgcc tcagtttact tcgtgctgcc ctgcttgggt
     2101 caagtacgcg gagcagttcc atccggagct tttagataat ctgtcttcct gccgctcacc
     2161 tcaaggtatg ttcggagcac tggtcaagaa ccacctggcc aaagagatga acatcgatcc
     2221 caaagacatc ttcttcattt caatcatgcc ttgtaccgct aagaagttcg aagccaaacg
     2281 ccctgagttt tcatcgggag atacgcctga ggtagatttg gtccttacca cccaggagat
     2341 cggcaacatg attaaagaag ccggaatcga tttcgccaat ctcgatgtgg aagccttgga
     2401 tatgcctttc ggcttcacgt ctggagcagg tgtaatcttc ggcagctccg gcggcgtagc
     2461 agaagcttct cttagagccg cagcggagat tatttccggc gaagaaatcg atgctgtaga
     2521 tttcaagcaa acaaggggtc ttacaggcct taaggaagcc gaagtggaga tctccggagc
     2581 caaattgaag atcgccgtag taaacggttt gaaaaatgcc aaagatctta ttgccaagat
     2641 tcaatctggc gaggtgcatt acgacctcat tgaagttatg gcttgccccg gaggctgcat
     2701 cggtggcgcc ggtcaaccta cgccaaacga taccaccacc cgcgagcagc ggatgaaggg
     2761 tctctacgaa gccgataagc tccaacagct gagaaagagc cagcagaacc ccatcgttca
     2821 gaagctctac agtcagtggc taggagaacc aggaagcgac attgctcacg aaaacctcca
     2881 caccacctac cagtctagac gccgcatcaa aggtcaaaac atcactctca gcg
//
LOCUS       DAOSDA010000226         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21970_length_2920_cov_2.993368, whole genome shotgun sequence.
ACCESSION   DAOSDA010000226 DAOSDA010000000
VERSION     DAOSDA010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21970_length_2920_cov_2.993368"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLW82_05795"
     CDS             complement(<1..487)
                     /locus_tag="PLW82_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOJ46821.1"
                     /translation="MAKQSHPYAAGRISVMERGLLGKSGLDRLLATQTVDEFYRALAE
                     LGWGDAYSQSDVEKIAANRIAEAYEFVRQNTPEPEVTDCFFIEYDVHNLKTLFKAKAL
                     GENISFELSPNGLIPRELLRACVENGNYHEIPIAELRESAAKLEVILSSDIDPVKIDG
                     EL"
     gene            complement(515..1117)
                     /locus_tag="PLW82_05800"
     CDS             complement(515..1117)
                     /locus_tag="PLW82_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOJ46822.1"
                     /translation="MTVEPIVKKILDDAKADAAQMLANAQSRIEERRKAQQDEFAKER
                     QKALEKAKNDAAVQHQRMLSMAQLEERKLDLAMKRDVIKLAFEKALEKMQSLPVDQVQ
                     RFMRTLLLQAKGDEEIIIDPKSEDVFTEQFITSINKELRASGKAGDLKLSKERREIGA
                     GCILSSGGVETNCTFAAILTMKQVELENEVASILFPHTDK"
     gene            complement(1160..1636)
                     /locus_tag="PLW82_05805"
     CDS             complement(1160..1636)
                     /locus_tag="PLW82_05805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOJ46823.1"
                     /translation="MNLAWGQILALVGAGLSVLGGGLGSAKAVRTAGQAGAGVVSENP
                     DTFGQVLVMQLLPGTQGVYGFLIAFIVLIKTGFLGGMIDLSLTQGLQIFLGCLPITIV
                     GGLSAVYQGQMAAAGITMTGRNPELGGRGITMTVMVETYAVLAFLASFLLVWFVAL"
     gene            complement(1693..>2920)
                     /locus_tag="PLW82_05810"
     CDS             complement(1693..>2920)
                     /locus_tag="PLW82_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOJ46824.1"
                     /translation="ELELAKLNRRSQTLSEEKRSLAQYLPALEAAYDLELIQRDRLRA
                     TQRLLCTEKAFLMHGWVPAEKVPALEKEIKKISPNTVLEVADPEDDDKPPVLLKNKWF
                     ASQFEGVVAMFSLPSYKGFDPTTVMGPFFVLLFGMMVGDAGYGIIMSILAALFLKFMK
                     PQGMIKQIAGILILGGVATAFWGFMFGGIFGIEITPWLFAPMDEPLLMLGLCFGVGFI
                     HLLAGLGFAAYMNIKRGKVLDAILDQGLWVLTLFGLPMLAVPQLAGVGKVLSIIGVGG
                     LALTAGRNAQGGPLKKLLSGLGSLYNLSGYLSDILSYARLFGMGLATGIIGMVFNTVA
                     GMIMGNPIGFVLGAAFLLVGHAFNLFINALGAFVHASRLQYIEFFSKFYEAGGRTFRP
                     LKVVTKYMQVKEDSLS"
BASE COUNT          776 a          777 c          644 g          723 t
ORIGIN      
        1 caagttcacc atcgatcttg actggatcta tatcagagga aagaattacc tcaagttttg
       61 cggcactctc cctcaactct gcaatgggaa tttcgtgata attaccattt tcgacgcagg
      121 cgcgcaaaag ctccctgggt atcaaaccgt taggcgatag ttcgaaggaa atgttctccc
      181 caagggcttt agctttgaaa agggtcttta gattgtgaac atcatactcg atgaaaaaac
      241 agtccgtgac ttctggctct ggggtgtttt gacgcacgaa ttcgtatgcc tctgcaattc
      301 ggttagcagc gattttttcc acatcggatt gagagtaggc gtcgccccaa cccaactctg
      361 ccagagcacg gtaaaactca tcaacagtct gggttgccag taagcgatcc aaacctgatt
      421 tacccaatag tccgcgttcc atgacgctga tgcgtcccgc ggcatatgga tggctttgtt
      481 ttgccaaggt taaacctcct tgcttcgagc agacttactt atccgtgtgt ggaaacaaaa
      541 tcgacgcgac ctcgttctcc agttcgactt gcttcatggt caatatggct gcaaaagtac
      601 agtttgtctc aaccccgccg ctagacagga tgcaaccggc gccgatttct cttctctctt
      661 tagagagctt taagtcgcca gccttaccag aagcccgcag ctctttattg atgcttgtaa
      721 taaactgctc ggtaaacaca tcctcgctct tgggatcgat aatgatctct tcatcgcctt
      781 ttgcttgaag aagcagggtg cgcatgaacc tttgcacctg atctacagga agtgactgca
      841 tcttctctaa agctttttcg aaagcgagtt tgataacatc tctcttcatt gccaaatcga
      901 gcttgcgctc ttcgagttgc gccatgctca acatgcgctg gtgctgcaca gcagcgtcgt
      961 ttttggcctt ttccaacgct ttctgtcgtt ctttggcaaa ctcatcctgc tgcgccttcc
     1021 gtctttcttc aatgcgactt tgggcgttgg caagcatttg ggcagcgtcg gctttcgcat
     1081 catccaatat ctttttgaca atcggttcta ccgtcatcac gcagccctcc ttcttatttc
     1141 tccactgtca tctgtgaact tatagtgcga cgaaccaaac cagtaagaag gaagccaaga
     1201 atgctagaac tgcgtaagtc tcaaccataa cggtcatggt aataccgcga ccacccagtt
     1261 cagggttgcg tccagtcatg gtaataccgg cagcggccat ttgaccctgg taaacggctg
     1321 agagaccacc aactattgta atcggtaagc aaccgagaaa gatctggagc ccttgtgtca
     1381 atgaaaggtc aatcattccg ccaaggaaac cagtcttaat gagcacgata aatgcaatca
     1441 agaaaccata aacgccttgt gtaccaggaa gcagttgcat aaccaatact tgaccaaatg
     1501 tatctggatt ctcactgact acacctgcgc ctgcttgacc cgcagtgcga acagcttttg
     1561 ccgaacccaa acctccacca agtactgaca atcctgcacc aactagggct aaaatctgtc
     1621 cccatgctaa attcatatac agttccccct atacttaaag tgatttatat tctaggctaa
     1681 cgccaaaaca aactaagaca aagagtcctc tttgacttgc atatacttag tgactacctt
     1741 caaaggacgg aaagtgcgac cacccgcttc gtagaacttg ctgaaaaact caatgtactg
     1801 tagacggctg gcatggacaa atgcacctag tgcgttgata aagaggttaa atgcatgtcc
     1861 cactagtagg aaagcagcac caagaacaaa cccgatgggg ttgcccatga tcatgcctgc
     1921 aaccgtgttg aaaaccatac cgataatgcc tgtggcaagt cccataccga agagacgggc
     1981 ataactcaaa atatcgctca agtaaccaga tagattatac agtgagccaa gtccactcaa
     2041 gagcttcttg agaggtccgc cttgtgcgtt ccttcctgca gtcagggcta agccgcctac
     2101 accaataatt gacagaacct tgccgacacc ggccagctga ggcactgcaa gcattggaag
     2161 gccgaacagc gtcagaaccc acaagccctg atccaaaatg gcatctaaaa cctttccgcg
     2221 cttgatgttc atgtaggctg caaagccaag gcccgctagc agatggatga aacctactcc
     2281 gaagcacagt ccgagcatca gaagaggttc gtccatagga gcaaagagcc aaggagttat
     2341 ttcgataccg aaaatacctc caaacatgaa tccccaaaaa gcggttgcta ctccgccgag
     2401 aatcaagatc ccagcgatct gctttatcat gccttgaggc ttcatgaact taaggaatag
     2461 tgcagctaga atgctcatga taatgccgta gccggcatcg cccaccatca taccgaacaa
     2521 aagcacgaaa aacgggccca tgaccgtcgt tggatcaaat cccttgtagg atggcagaga
     2581 gaacatcgcc acgacgcctt cgaactgtga agcaaaccac ttgttcttca acagcaccgg
     2641 tggtttgtcg tcatcttccg gatcggcgac ctccaggacg gtgtttggcg agatcttctt
     2701 aatctctttt tccaaagcag gcactttctc agctggcacc cagccgtgca taaggaacgc
     2761 cttctcggtg cagagcagtc tttgagtagc acgcaggcga tctctttgga tcagttctaa
     2821 gtcgtatgca gcctcaagcg caggtagata ttgggctaga ctccgctttt cctccgacag
     2881 agtctgagat cgtctgttga gcttagctag ttcaagctcg
//
LOCUS       DAOSDA010000227         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_21989_length_2918_cov_3.143556, whole genome shotgun sequence.
ACCESSION   DAOSDA010000227 DAOSDA010000000
VERSION     DAOSDA010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21989_length_2918_cov_3.143556"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLW82_05815"
     CDS             complement(<1..193)
                     /locus_tag="PLW82_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46825.1"
                     /translation="MDFLEQRGLPLTEENLATWQEDLEAREQAAKQRNQELRNARAEV
                     VRDFNQLKSKEAELFRARAT"
     gene            complement(206..370)
                     /locus_tag="PLW82_05820"
     CDS             complement(206..370)
                     /locus_tag="PLW82_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46826.1"
                     /translation="MSAKLEKRRMALLKREEALKKLEEENYVLELEIEQLKREMKVLE
                     MRIEYLSSNE"
     gene            complement(394..1263)
                     /locus_tag="PLW82_05825"
     CDS             complement(394..1263)
                     /locus_tag="PLW82_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOJ46827.1"
                     /translation="MSKRVRIIADTGSDLPQSLCKQRGIELVGMGVEIDGVARNGLEV
                     SAAEVYQKMREGSVPHTSQVSYTDLEAIVKKTIERGEIAYYVCFSSRLSGSYQTGHML
                     KQELNHPDFILFDTKAASIGLGMMVLEAAEMAENGESAEKITERLEYLSGRMEHIFAV
                     DDLVYLKRGGRISSMKAIIGGMLKIKPILHFIDGAIHQFDKARSTKQVIGRMVDIMAE
                     RADDIDSQKVIGISHADCPDVAEELKKAIVDRFGQKEFIVHEIGPVIGAHAGPGTIAL
                     FFFSKKQGGGLEA"
     assembly_gap    1671..1680
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1681..2367)
                     /locus_tag="PLW82_05830"
     CDS             complement(<1681..2367)
                     /locus_tag="PLW82_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOJ46828.1"
                     /translation="MSLKPCGFRFGLKDCSTTLRRKTVLDDFAGKKVTLMVDCHHENC
                     IDNSCVAKVPIFAKLSPEEMLEVAKVTVKRVFAKGEFIFFQGDLIDKLYVIHEGSVKV
                     SRMSEEGREQVIRILGPGDFLGELVLFAPSPAGNSAEALEKTVVCEVESSKIKELMLS
                     SSKIAVKLLEEMSRRLEEAENLLESISLLSVDKRIVKSLLEFAGETDEVVLPISKRDL
                     AAQLSITQETL"
     gene            complement(2477..>2918)
                     /locus_tag="PLW82_05835"
     CDS             complement(2477..>2918)
                     /locus_tag="PLW82_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46829.1"
                     /translation="GQALFSTVHLKPELVEVLGKVWRRPAMPTFRDPLLAAKSIIENL
                     RDRFTGLGSQIGIDSEVNQEIVLGLGSVAEAIASCNLRPWDSRDLQEGSLEELEQLEQ
                     VLSLIRPLKRLLSKLEPPTRERALSDLQKAERLIQVLRHYAMPI"
BASE COUNT          658 a          812 c          605 g          833 t
ORIGIN      
        1 ttgtagctct tgctctaaag agctctgctt ccttgctctt cagctggttg aagtccctca
       61 ccacttcagc tcgcgcattt ctcagttcct gattgcgctg cttggcagct tgttctcgcg
      121 cttcgagatc ttcctgccaa gtcgctaaat tttcttctgt caaaggcagg cctctttgct
      181 cgagaaagtc catgacttct tcctcctact cgttgcttga tagatactca atccgcatct
      241 ctaagacctt catttcccgc ttcagctgct ctatctccag ctctaaaacg tagttctctt
      301 cttccagttt cttaagggct tcttcgcgtt ttaaaagggc catccttctt ttttccagct
      361 ttgcactcat aaccaacacc ctttatcgtg aaattatgcc tccagacccc caccctgctt
      421 cttggagaag aaaaacaaag caatggtccc cgggccggca tgtgcaccga ttaccgggcc
      481 gatttcatgt acaataaact ccttctggcc aaacctatcg acaatggcct tcttcagttc
      541 ttcggcaaca tccgggcagt ctgcatggct gatgccgatg accttttgcg agtcgatgtc
      601 gtcagcgcgt tctgccataa tatccaccat acgcccgata acctgttttg ttgatctggc
      661 tttgtcaaat tggtgaattg ctccgtcgat gaagtggaga atgggcttga ttttcagcat
      721 gccgccgata atggccttca ttgaactgat gcgtccgcca cgcttgaggt aaaccaggtc
      781 atctacggca aaaatgtgct ccatgcgacc tgataggtac tccagccgct cggttatctt
      841 ctctgcgctt tcaccatttt ctgccatctc tgcagcctcg agcaccatca tacccaagcc
      901 tatgctggca gctttcgtgt cgaacaggat gaaatccggg tggttcagct cctgcttcag
      961 catgtggccg gtttgatagg aaccgctcaa cctgctggaa aagcagacgt agtaggctat
     1021 ctctccccgt tcgatagtct ttttcactat agcttcaaga tcggtgtagg aaacctggct
     1081 ggtgtgagga accgagcctt ctctcatctt ctgatagacc tctgctgcag aaacctccaa
     1141 accgtttctt gctacaccgt cgatttcaac acccatccca acaagttcga tgcctcgctg
     1201 tttgcacaag ctctgcggaa gatctgaacc agtatcggca atgatcctta ctctctttga
     1261 caagcgaaca acctcccaaa acgctgtttt atgtccatgc tttccgtacc aggtgtattg
     1321 ctgtcagttt acgaaaaaca aaacttcttc cctaatctcc tacccgtttt ttacttattg
     1381 gtaaccttta cgactattat attctttttc tgcgagttta agcaactttc acctggtact
     1441 agctttaagt tctaagaaca agtacttact tccttctact tatctctatt tgttattaaa
     1501 cgaacaaagg agatgcctat aagaaaaaga aactactaat cgaagtagtt tctaaattct
     1561 ttattcatca agtatatcct tcagcttcgc tcggtcaaga agccggatct tcctctgccc
     1621 gcttagcgct accaggccct cctcttgaag ggctgtaaac ttcctgctca nnnnnnnnnn
     1681 caaggtctcc tgggtgatgc tgagctgtgc cgcaaggtcc ctcttgctga tcgggagcac
     1741 aacctcatcg gtctcaccgg caaactccag cagtgatttg acaatccgct tgtcaacact
     1801 gagaaggctg atactttcca ggaggttttc ggcttcttcg agcctccggc tcatctcttc
     1861 aagtagtttt acggcaattt ttgaggatga aagcatcagc tccttgatct ttgagctctc
     1921 cacctcacac acaacagtct tttccaaagc ctctgcactg tttcctgcag gtgaaggtgc
     1981 aaagaggacc agctctccga ggaagtctcc gggacctaga atcctgatta cctgttccct
     2041 accttcctca gacatcctcg ataccttgac gctaccttcg tggataacgt acagcttgtc
     2101 tattagatcg ccctggaaga agatgaactc gcccttggca aaaacccgct tcaccgttac
     2161 cttagccact tccaacatct cctcaggaga gagcttggca aatattggca ccttggcaac
     2221 gcagctgttg tctatgcagt tttcgtgatg gcagtctacc atcaaagtca ccttctttcc
     2281 tgcgaaatcg tctaaaaccg tcttgcgtct taaagtcgtg ctgcagtctt ttagaccgaa
     2341 ccggaaaccg caaggcttta agctcacgat ctatataccc tctaagcggt ttttgtaacc
     2401 gcagcctttt ctcctgccgc gcttactggc gtatataaga ggtggaaagt ggcagctttc
     2461 cacctaagca tttcaatcaa atgggcattg cgtagtggcg gagcacctgg attaggcgct
     2521 cagctttctg taaatcgctt agtgcccgct ctcttgtggg aggttctagc ttgcttaaga
     2581 gcctcttcaa aggcctaatt agagatagga cctgttcaag ctgctccaac tcttcaagcg
     2641 agccttcctg gagatcgcga ctgtcccacg gacgtaggtt gcagctggca atagcttctg
     2701 caacgctacc taagcctaac acgatctcct gatttacctc agagtcgatg ccgatctggg
     2761 agcctaaacc cgtgaatctg tccctaaggt tttcgatgat gctttttgct gcaagcagcg
     2821 gatcgcggaa ggtgggcatg gccggtctac gccaaacctt gcctaaaacc tccaccagtt
     2881 ccggtttcaa atgaacagtg gaaaacaggg cctgacct
//
LOCUS       DAOSDA010000228         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22035_length_2913_cov_2.981106, whole genome shotgun sequence.
ACCESSION   DAOSDA010000228 DAOSDA010000000
VERSION     DAOSDA010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22035_length_2913_cov_2.981106"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLW82_05840"
     CDS             <1..538
                     /locus_tag="PLW82_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46830.1"
                     /translation="SGTERGRGILSILISAGAVYLISQLFDFGPEAGSYTFIDPMYLF
                     GALAGVVAYLIGRSRRAAFIGGIGSILLVESITIISRQITEGGPANLGTAGFFTGALI
                     AVIVAIFLAEIVGETRELMQGGPEKGDDYPTGLKKGNAPRDVEARATHALRINKPILV
                     LEIGDGGDVRVFLEENAQ"
     gene            535..1569
                     /locus_tag="PLW82_05845"
     CDS             535..1569
                     /locus_tag="PLW82_05845"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HOJ46831.1"
                     /translation="MNKQIRLLLVLSLCTVFCFSSPAFGYHLDEVRDVVDEAGNLVTK
                     ATIPLAEGDIYISSDNTEWEVIGERDGKYIVKKNGVIDLSAYLKMPLDLGIQAEGSQP
                     VIGIYHTHSSESYSPGPASLPYPGEIYQVGETLKNALEKKGFKVVWSQANHNPHDGSA
                     YIRSRDTAAKLLEENPIAVIDVHRDAVPDREFYTTELDGQSVAKVRIVTGKQNQNRQS
                     NFEFAKAMKAKADEALPGIMEGIFWAKGNYNQDLGPRMILLEFGTHVLTLEEAKVAAE
                     KYADVIAASLPVGGGKAERSAAGSSVVWVILLAVAALGIFLFINKKGFGSLKNLAKEF
                     THSLRKGRRD"
     gene            complement(1576..1719)
                     /locus_tag="PLW82_05850"
     CDS             complement(1576..1719)
                     /locus_tag="PLW82_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46832.1"
                     /translation="MRQSENLITKNAVCKDRTIATSLKAFSASSLFELRLFGAEGFFT
                     VRM"
     gene            1996..>2913
                     /gene="der"
                     /locus_tag="PLW82_05855"
     CDS             1996..>2913
                     /gene="der"
                     /locus_tag="PLW82_05855"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOJ46833.1"
                     /translation="MEKGTVAIVGRPNVGKSTLFNRICGQRKAIVEDTPGVTRDRLYG
                     TAEWNGKEFSVIDTGGILLGDDEPFLREIRMQAELAVEEADVIIFVVDGREGLTPTDQ
                     EVAEILRTKGKNVVVAANKLENFQGTEIWDFYSLGLGEVVRISSEHGIGTGDLLDRVT
                     ELLPEAKATEESSAIKVAIVGRPNVGKSSLVNNLLGEERVIVSDIPGTTRDAVDVPFS
                     YEGQDYILIDTAGLRKKGKIWESVERYSVIRSLRAVDEADVVLHIIDLTEGVTEQDTK
                     IIGYAHDQGKALILAGNKWDLIEKETGTFE"
BASE COUNT          776 a          678 c          810 g          649 t
ORIGIN      
        1 aagcggcaca gagcggggta ggggcattct gtccattctc atcagcgccg gagcggtcta
       61 tttaataagt cagctcttcg actttggacc ggaggccgga agctacactt ttatcgaccc
      121 catgtacctt ttcggggctc ttgccggtgt tgtagcgtac ctcatcgggc ggtcgagaag
      181 ggcggcgttt atcggcggca tcggcagcat cctcttggtg gaatcgatta ccatcatctc
      241 caggcaaatc actgaagggg ggccggcgaa cttgggtact gcaggctttt tcaccggcgc
      301 cttaattgcc gttatagtag ctatcttctt ggcggagatc gtgggagaga cccgtgagct
      361 gatgcaaggc ggtccggaaa aaggcgacga ctaccccact ggactaaaaa aaggcaatgc
      421 tccaagggac gtagaggcca gggctaccca cgctctaaga ataaacaagc cgatcttggt
      481 tttggagatc ggcgacggag gagacgtacg agtctttttg gaggagaatg cgcagtgaac
      541 aaacagatcc ggctgctcct tgttctatct ctttgtaccg ttttctgctt cagctcccca
      601 gcgttcggct accacctcga tgaagtccgc gatgtggtag atgaagctgg gaacctggtc
      661 acaaaagcca ccatccccct cgcagaaggg gatatctaca tctccagcga caacaccgag
      721 tgggaggtca ttggagaacg ggacggaaag tacatcgtaa agaaaaacgg cgtcattgat
      781 ctcagcgctt acttgaaaat gcccctggac ttgggaatcc aagcggaagg gtcgcagcct
      841 gtcatcggca tctaccacac ccacagcagt gaatcttact cgcccggacc tgcaagtctc
      901 ccctatcccg gtgaaatcta ccaggtaggc gagactctga aaaatgcgct ggaaaagaag
      961 ggattcaagg tagtttggtc ccaggccaat cacaatcccc acgatggttc ggcgtatatt
     1021 cgctccagag acaccgcagc taagctcctg gaggaaaacc ctattgcggt tattgacgtc
     1081 catagagatg cagttccgga ccgagagttc tacaccacag agctggacgg ccagtcggta
     1141 gccaaggtga ggatcgtaac cggcaagcag aatcagaaca ggcagagcaa ctttgagttt
     1201 gcaaaagcta tgaaggctaa ggccgacgaa gcacttcccg ggatcatgga agggattttc
     1261 tgggctaaag gcaactacaa ccaagaccta ggtccgcgaa tgatcctgct cgagtttggc
     1321 actcacgtgc tgactcttga agaagccaaa gtggcagcag agaaatacgc tgatgttatc
     1381 gccgcgtctc tccctgtagg cggagggaaa gcggaacgca gtgcagctgg ctctagcgtg
     1441 gtttgggtga tccttctagc tgtagcagct ctcggcattt tcctgtttat caataaaaag
     1501 ggctttggca gtttgaagaa tcttgccaaa gagtttaccc actcattgag aaagggtagg
     1561 agggattagt cagctttaca tcctgacagt gaagaaccct tcggctccga acagcctgag
     1621 ctcaaataga gaagaggcag agaaggcttt cagacttgtg gcaatggtgc ggtctttaca
     1681 gaccgcattt ttcgtgatta agttctcaga ttgcctcatg tcaccttgtt ttctcaatgg
     1741 tgctactgcc cttttaaaag cagccacctt atgataatat gaatgtgcga ttacttgata
     1801 aatcattgtg acattacgac ttcacctctt tcagtaggtg ccgagtttga caaaaaatgc
     1861 tcaaaggcac gtacccacaa gttggggcac aaaagatagc agagaggagg cacagctgga
     1921 tattgctact taacggggaa aatgttactt aagtgaagtg tacattttcc aagaggagca
     1981 atatactttt gaaatatgga aaaaggaact gttgcaattg taggaagacc gaatgttggc
     2041 aagtcaacgt tatttaaccg aatatgcggc cagagaaagg ccattgttga agacacgcct
     2101 ggcgtaacca gggatcgcct ttacggcaca gcagagtgga acggcaagga gttcagcgtg
     2161 atcgacacag gaggtatcct cctcggcgat gacgagccgt ttttgcggga gatccgcatg
     2221 caagctgaac ttgcagtaga agaagccgat gtgatcatct ttgtagtgga cggaagggaa
     2281 ggactgaccc ctaccgacca ggaggttgca gagattctga ggaccaaagg caaaaacgtc
     2341 gtagttgccg ccaataagct ggagaacttc caggggacgg agatttggga tttctattcc
     2401 ctgggtctcg gcgaagtggt tcgcatctcg tcggaacacg gtatcggcac aggagacctg
     2461 ttggaccggg ttacagagct tcttcctgaa gcaaaagcta cggaagagag cagtgccatt
     2521 aaggtggcca ttgtggggcg gcctaatgtg ggcaagtcgt ctctggtcaa caaccttctc
     2581 ggagaggaaa gggttatcgt cagcgatatt cccggcacca ccagagatgc agtggacgtg
     2641 ccgttttcct acgaaggcca ggactacatt ctcatcgaca ctgccggtct gagaaaaaaa
     2701 ggcaagattt gggagtcggt ggaacgctac agcgtcatac gttctctacg agccgtcgat
     2761 gaggccgatg tggttttgca tataatagac ttgacagaag gagttacgga acaagacacg
     2821 aaaataatag gctatgccca cgatcagggc aaagctttga tactagcagg taataagtgg
     2881 gacttgatcg aaaaagagac aggcaccttt gaa
//
LOCUS       DAOSDA010000229         2907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22101_length_2907_cov_3.279804, whole genome shotgun sequence.
ACCESSION   DAOSDA010000229 DAOSDA010000000
VERSION     DAOSDA010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22101_length_2907_cov_3.279804"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1384
                     /gene="rsmB"
                     /locus_tag="PLW82_05860"
     CDS             38..1384
                     /gene="rsmB"
                     /locus_tag="PLW82_05860"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOJ46834.1"
                     /translation="MTKNNEYYWAREKAALALVAVEGGMWAKDALEKFTGEDKTTDAL
                     LTEIVYGTLQNLLFLDHVLSFYLKHPLEKLTPHVRNILRSALYQLHFLDRVPKSQVIY
                     DAVRLAGRFGHKGVQGLVNGVLRSFLREKKWQLPTPVLDRLSVQYSHPKWLVERWVEE
                     LGAEETERLLKWNNSRHPLCVRVNSLVIDADEYVQLLDEQGIKYFRDHDIPAAVYLPK
                     SGRVGDLPLFAEGAVQPQSKASMLVALILGAQPHEVVLDLCAAPGTKTGHIAELMKNT
                     GKITAVDISEERLVLARENLARLKVKNVQFVQSDATQLELPQFDRVLVDAPCSGLGTL
                     AHRPDARYQKTEESLRSLPELQLQIINRAAKLLKPGGVLVYSTCTTEKAENWQVVENF
                     LQLNPDFVEEEEIPQITGLVKTQHGFQSYSHRTDTDGFYYCRLRKLKQPYKQQQYC"
     gene            1591..2373
                     /gene="spo0A"
                     /locus_tag="PLW82_05865"
     CDS             1591..2373
                     /gene="spo0A"
                     /locus_tag="PLW82_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HOJ46835.1"
                     /translation="MISVLIVDHSRELCDIIAKYLSLQPDMQICGVCYDGRSAVNLAR
                     ELRPELVLLDLGLPLLDGLGVLDALRGELPKVLVITAFPREDIIQKALDLGASYCLLK
                     PFYLSVLARTIRDVYSSSTEMVMDNFNLKSFLDNRELARYIEDFLTAAGIRQQLKGYK
                     YLKEALYLALDNQPLVLDALTKVLYPALAEKFNTTPSSVEAAIRGAITSAWQSDKAAL
                     AKLMRKKDECDGRKKPTTSEFLTLARRQIQVELREAQKEVLY"
BASE COUNT          805 a          684 c          716 g          702 t
ORIGIN      
        1 caaatactta gactgttgat tttgaggggt tcacgacgtg actaagaata acgaatacta
       61 ctgggcaagg gagaaagccg cccttgccct tgttgctgtt gaaggcggca tgtgggccaa
      121 agacgctctt gaaaagttca caggtgaaga caagaccacc gacgctctcc ttacagagat
      181 tgtctatggc actttgcaga atctgctgtt tttggatcat gttctcagct tctacctcaa
      241 acatcccctg gaaaagctga ctccacatgt cagaaacata ctccgttcgg ctctgtacca
      301 actgcacttc ctcgatcgag tacccaaaag tcaggttatc tatgatgcag ttaggctggc
      361 agggcgcttc ggccacaaag gcgtgcaagg gctcgtcaat ggagttctcc gtagttttct
      421 gagggaaaaa aagtggcagc tgccgacgcc tgtgctggac aggttgtctg tgcagtactc
      481 gcatcccaag tggcttgtgg aacgttgggt ggaagaacta ggtgcagaag agacagaacg
      541 actccttaaa tggaacaaca gcaggcaccc cctctgcgtc agggtaaact ccttggttat
      601 agatgcagat gagtacgtac aacttctcga tgaacagggc attaagtact tccgcgatca
      661 cgacatcccc gctgcagttt accttccaaa aagcggacgg gtcggagatc tcccgctgtt
      721 tgcagaagga gctgtacaac ctcagtccaa ggccagcatg ctggtggcct tgattttggg
      781 tgcacagccc cacgaggtag tattggacct ctgcgccgct cctggaacaa agactggtca
      841 tattgcagag ctcatgaaaa acactgggaa aatcaccgcc gttgatatca gcgaagaaag
      901 gttagttctt gcaagagaaa accttgccag gcttaaggta aaaaacgtgc agtttgttca
      961 gagcgatgct acacaattgg aactcccaca gtttgatcgg gttcttgttg atgcgccctg
     1021 ctcgggcctt gggactcttg cccacagacc cgatgccagg taccagaaga ccgaagaaag
     1081 cttaagaagc ttgccggaac tacagcttca gattatcaac cgagcagcga aactgctgaa
     1141 gccaggtgga gtactggtgt acagcacctg cactacagag aaagctgaga attggcaggt
     1201 tgtagagaac ttcctccagt tgaatccaga tttcgtagag gaggaagaaa taccccagat
     1261 tacaggtctt gtgaagactc aacacggatt tcagagctac tctcacagaa ccgacaccga
     1321 cggtttctat tactgccgtt tgcgcaaact taaacaacca tacaaacagc aacaatattg
     1381 ctaatcattt cacaaaagat atttatgtaa caaattacct tgtactaaaa cttcatagtg
     1441 ctaaaataat actataaagt tccatccttg aatcctagta ccgtgtcgcg caaacggcgt
     1501 ggttggtttg gagaaagaca gggtctggag tttcgtcaga tctgcttttt ctggacaaac
     1561 tccagtctgg atagtagagg aggaaagctt atgatctccg tgttaattgt tgatcacagc
     1621 cgagagctct gtgacatcat tgccaagtac ctttcgctgc agccggacat gcagatctgc
     1681 ggcgtctgct atgacggtag gtcagcggta aacctagcta gagagctccg accggagctg
     1741 gttctcttgg atctcggact ccccctcctg gatgggctcg gagttttaga tgctctgcgg
     1801 ggagaattgc caaaagtatt ggtaataacg gcttttcccc gcgaggacat cattcaaaaa
     1861 gcgttggacc ttggagcctc gtactgtctg ttaaaacctt tctatctgtc agttttggcc
     1921 agaaccataa gagatgtcta ctcatcgagc accgaaatgg ttatggataa cttcaacctc
     1981 aagtccttcc tagataaccg tgagctcgca cgatacatag aagactttct gacagccgct
     2041 ggcattaggc agcagctgaa aggatacaag tacttaaaag aagctcttta tctggctctc
     2101 gacaaccaac ctctggtctt agatgccctt actaaagtcc tctatcctgc cttagcagaa
     2161 aagttcaaca cgactccgag cagcgttgaa gctgccattc gcggggcaat tacgtccgct
     2221 tggcagagtg acaaagccgc gctggcaaag ctgatgcgca aaaaagatga atgcgacgga
     2281 agaaaaaagc ccacaacgtc cgagttttta accttagctc gcagacagat ccaagtagag
     2341 ttaagagaag cacaaaagga agttctgtac tgacgggcac ctgccaagca gtaagtaaaa
     2401 atgatagata ctttcaagaa gcaccaatcg tctcggttgg tgctcttttt atgcggtttt
     2461 tcccagcaag tacccgataa acagttttcg cgtttcaact gtttaccaca aagagagcat
     2521 atcaaagcga ggagttccct tttttcgata catcagtcac gagagggact ttagcatcat
     2581 agactgttac tagcttaggt aagagcagaa aagcccgata cagctccgga tcgcaagacc
     2641 aaatggctca cagggagtat atcctgtaaa accggagggt ttacccttcg ctgccacttg
     2701 atctggtctt tggcttttca gtctcgtatc caaggggagg aatcggctat gaattttgca
     2761 gaggagctgc gcttgtacga aagagtgcag aaagctgcaa gttattttca gatgcaagta
     2821 tacagctaca aaaaccatct ttgggagacg tctcggggac ctttgcagct gaaaacttgg
     2881 gagcgtggtg attttgacga gacgagg
//
LOCUS       DAOSDA010000230         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22114_length_2906_cov_2.774114, whole genome shotgun sequence.
ACCESSION   DAOSDA010000230 DAOSDA010000000
VERSION     DAOSDA010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22114_length_2906_cov_2.774114"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="PLW82_05870"
     CDS             complement(<1..1236)
                     /locus_tag="PLW82_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ46836.1"
                     /translation="MRDFELRDLTINPQRARALIGIVAAFFLVLLGRLWYMQIIKGDE
                     YSEVAINQRTRVISLFAPRGMIYDRNHNPLVTNRMAFTVSVLPDAAGEIRRSPEKTAL
                     LAEILEISQDYVLNMLSPQRRDRLGYEPYRIAEDVGAEKAMQIYEQSWRLPGVIVEKV
                     PVRHYVYDSFAAHVLGHVGMISASELRDWSAYGYGSNHKVGKYGIERFYEFELKGTDG
                     AVEIEIDARQMPVREVGRQEPVRGNDLILTLDFELQAIAEEVLDRKVEEMKDSDRPIN
                     GAAVIIIDPNTGEVLTLLSYPDFNPNTYNLDFTKLESDSRSPLLNRVTRGAYPPGSAF
                     KPIVVASALHEGIISRSTTWFDAPYPHGSGGWEPEYGKRCVYGPHGTIDILRGLQVSC
                     NIPFYEIGKELGIDKLSELA"
     gene            complement(1249..1725)
                     /locus_tag="PLW82_05875"
     CDS             complement(1249..1725)
                     /locus_tag="PLW82_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46837.1"
                     /translation="MRRWPYLIAAVVLSILEISFFPFLKGIVPAFSLLLAVLLALKAD
                     LRLGTACGILAGFVIDFTFGFGVPQMAITYGLIAYLIGRLASHIVDLGLLTYLVAALV
                     ATVSTSLMAAFFSLAFGYSGWQLVINWKNIGGTLLFNALAAVLVYLFARKRLPSRN"
     gene            complement(1722..2579)
                     /gene="mreC"
                     /locus_tag="PLW82_05880"
     CDS             complement(1722..2579)
                     /gene="mreC"
                     /locus_tag="PLW82_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOJ46838.1"
                     /translation="MSKKLQLLLSIFIGVLCLVLIFATTIPRERLTFLEKIWRDATFP
                     VQKAFSSVGKALKNSYTFVTSLTTAQSEVSRLEKELEETRMELERFAEIRLENERLKL
                     LLGFKEESDQILVPSRVIGRDPRTWLRSIIVDKGERHGVREGTPVVSHAGVIGHVTFT
                     SAFTSQVTLILDPKIAVGGIVQRTRDFVVVGSADDTGHLTVTPLYQREEGDSSTTSSV
                     IDIKEGDVVVTSGFGGIYPKGLNIGVVTEVHMGTEGISAKLKPSAEFASLEEVFVLLT
                     LQPAGEGES"
     gene            complement(2630..>2906)
                     /locus_tag="PLW82_05885"
     CDS             complement(2630..>2906)
                     /locus_tag="PLW82_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOJ46839.1"
                     /translation="REIQEALRESVQNIVDSVRMTLESTPAELAADIMERGIMLAGGS
                     SQLRGLDKLISKETGMPVLKPDEPLNCVVYGTGKALEDYDLLERVSC"
BASE COUNT          659 a          804 c          739 g          704 t
ORIGIN      
        1 ggcaagctcc gacagcttgt ctatgcccag ctccttgccg atttcataga agggtatgtt
       61 gcaggagacc tgcagtcccc tgagaatgtc gatggttccg tgggggccgt agacgcagcg
      121 cttgccgtac tcgggctccc aaccgccgga tccgtgggga taaggcgcgt cgaaccacgt
      181 ggtggatctg gagatgatcc cttcgtgcag ggcgcttgca actactatcg gtttaaaggc
      241 cgatcctgga gggtaggcac ctcttgttac ccgattgagt agaggcgagc gtgagtccga
      301 ctcaagcttg gtaaagtcca ggttgtaggt gttgggatta aagtcgggat agctgagaag
      361 cgtcaggacc tcgccggtgt tgggatcgat aatgatcact gcagcgccgt tgataggcct
      421 gtctgagtcc ttcatctctt cgacttttct gtccaggact tcttcggcga tagcctggag
      481 ctcaaagtcc aaggtgagga tgaggtcgtt cccccgaacc ggttcctgtc ttcctacttc
      541 tctcacaggc atctgcctgg catcgatttc tatctctacg gcaccgtctg tgcccttgag
      601 ttcaaactcg tagaagcgct caataccgta cttgccgacc ttgtggttgg agccgtaacc
      661 gtaggccgac cagtcgcgga gttcgctagc gctgatcatg cccacatggc caaggacgtg
      721 tgcggcgaag ctgtcgtaaa cgtagtggcg gactgggacc ttttcaacga tcactccagg
      781 aagccgccag ctctgctcgt agatctgcat ggctttctcg gcacctacat cctcggcgat
      841 tctgtagggt tcgtacccca accggtctcg cctttgcggc gagagcatgt tcagtacgta
      901 atcctgggag atttccaaaa tctccgcaag gagtgcagtt ttttccggcg accttctgat
      961 ctctcctgcc gcatcaggaa gcacgctgac ggtaaaggcc atgcggtttg tcaccagggg
     1021 attgtggttg cggtcgtaga tcatccccct aggagcaaaa agcgaaatga ccctggttct
     1081 ctggttaatg gccacctcgc tgtactcatc tccctttatg atctgcatgt accacaagcg
     1141 tccgaggaga accaggaaaa aagctgcaac aatcccgatt aatgctctcg ctctctgagg
     1201 gtttatcgtt agatctcgca gctcaaaatc acgcatggtt tttctcctct agtttctcga
     1261 tggtagtctt tttcttgcaa ataggtaaac gaggacagct gctagagcgt taaaaagcag
     1321 cgtgcctccg atgtttttcc agtttataac cagctgccag ccgctgtagc cgaaagccag
     1381 cgaaaagaac gctgccatga gcgatgtaga aacggtggca accagcgctg caacaagata
     1441 agtaagtagg cctaaatcga cgatatgact tgcaagacgg cctatgagat aggcgatcag
     1501 accgtaggtt atggccattt gaggcactcc aaagccaaag gtaaagtcga tgacaaaacc
     1561 ggccaaaatg ccgcaggctg tacccagtct gagatctgcc tttagcgcca gcagcactgc
     1621 caaaagcagg ctgaaggcgg gcacgatgcc ttttagaaaa ggaaaaaagg agatctccag
     1681 aatgctgagg acaactgccg caataagata cggccatctt ctcatgactc tccctccccc
     1741 gctggctgca aggtcaggag aacgaagact tcttcgaggc tggcaaactc tgcagatggt
     1801 ttaagctttg cggagatgcc ttcggtaccc atgtgaactt cggtaactac tccgatgttc
     1861 agccctttag ggtagatgcc gccaaagccc gaggtcacca ccacatcacc ttctttaatg
     1921 tcaatcaccg atgatgtagt ggacgagtca ccttcctccc tctggtagag gggagtgacg
     1981 gtgaggtggc ccgtgtcatc tgcactgccc acaacaacga agtccctcgt ccgctgcaca
     2041 atccctccca ctgctatctt cggatctaaa atcagggtta cctgagaggt aaaggcgctg
     2101 gtaaatgtca catgtccgat tactcccgcg tgggagacca caggagtacc ttctcgcacg
     2161 ccatgcctct cgcctttatc gacaataatg ctcctcagcc acgtcctggg gtctctgccg
     2221 atgactctgc tgggaacaag gatctgatcc gactcttcct taaagcctaa aagcagcttc
     2281 agcctttcat tctccagacg gatctctgca aacctttcca gctccatcct ggtctcttct
     2341 agctcttttt ccagcctgct cacttcgctt tgggctgtgg tcagcgaagt gacgaaggtg
     2401 taggagtttt tcagcgcctt acccacagag gaaaaagcct tctgcactgg gaaggttgca
     2461 tcccgccaaa tcttttccag aaaggtcagc cgttctctgg gaatggttgt ggcaaaaatc
     2521 aagactaagc aaagtacccc aataaaaatg gagagcagaa gctgtagttt tttcgacatg
     2581 cctagctgcg gccagctatg ccgctctccc cccttttatc tgaactcaac taacacgaaa
     2641 ctcgctccaa aaggtcgtaa tcttctagtg ctttgcctgt gccgtaaacc acacagttca
     2701 gaggctcatc tggtttcagc acgggcatgc ccgtctcctt ggagatcagc ttatcaaggc
     2761 cgcgaagctg cgagctacct ccggctagca tgattcccct ctccataata tccgctgcaa
     2821 gttctgcagg cgtgctctct aaggtcattc tcaccgagtc gacaatgttc tgtacgctct
     2881 cgcggagggc ttcctgtatc tccctt
//
LOCUS       DAOSDA010000231         2903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22144_length_2903_cov_2.885183, whole genome shotgun sequence.
ACCESSION   DAOSDA010000231 DAOSDA010000000
VERSION     DAOSDA010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22144_length_2903_cov_2.885183"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1824
                     /gene="pulA"
                     /locus_tag="PLW82_05890"
     CDS             <1..1824
                     /gene="pulA"
                     /locus_tag="PLW82_05890"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOJ46840.1"
                     /translation="KLGDGGVSAVIPMEKAEGGTWKKEVFGDLDGQRYNYIVRHHDVE
                     YEVVDPYARAVTVNGQMGVVVDLSKTNPKGWNDTPDPELHSAVDAIVYEVHVRDFSIS
                     PDSGIQNKGLYLGFTELGTTTSGSVYTGVSHLKELGITHVQLLPIYDFATVDETRPFD
                     SYNWGYDPLNFNAPEGSYATDPKDPIARIRELKQLIQSLRENGIRVVMDVVYNHTYYT
                     EESAFNKIVPGYYYRTDRDGVYSNGSGCGNELKTERKMVRKFIVDSMCYWAEEYKIKG
                     FRIDLMGVFDIETVNTLREKLHKIDPSILIYGEGWNGGPSLLDDNLRAVKGKAHEFLC
                     IGVFNDDFRDAIKGNVFRAENRGFISGAYGMEESIKCGITAAVDHPGVDYKRVLYSRA
                     PFCVNPFQSINYAEAHDNLTLFDKLTRSNPEDDEPLTIARQKLAGAVILLAQGIPFLH
                     AGQEFCRTKFGDYNSYRSGDFINQIVWANKVRYYDVFRYYQGLIALRKAHRAFRLRTR
                     DEIVRHLEFMPPQGPLVVKFLLKDHAGGDPWEKIVVFLNGARTAKQVVLPGEDWVIVA
                     DARTAGTAKIGQVKGSEVLLPRISCLVLVDRKSFEEGKKES"
     gene            1832..>2903
                     /gene="glgB"
                     /locus_tag="PLW82_05895"
     CDS             1832..>2903
                     /gene="glgB"
                     /locus_tag="PLW82_05895"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HOJ46841.1"
                     /translation="MVKHISCVHPSEYDRYLFCEGSHCESYRFMGAHLCEENGTPGVR
                     FTVWAPNAKSVSVIGDFNSWNESAHPMERLEESGLWQAFVRGAKEGDLYKYAVFTSDG
                     QKLIKADPYAFYSELRPKTASVVWDFKKYIWSDGDYLRRRSSQAVYKEPVNIYEVHLG
                     SWKQKPLGDFLSYRELADELIPYAYDMGYTHIEILPLAEHPFDGSWGYQATGYYSATS
                     RYGTPEDLMYFVDKAHSYGLGIILDWVPGHFCKDEHGLRLFDGTPAFEYDEPWRSENL
                     GWGTANFDLGKGEVRSFLISNAVFWFDIFHIDGLRVDAVANMLYLDFGREPGSWKPNI
                     YGGRENLEAIEFLRKLNEAVFGR"
BASE COUNT          739 a          698 c          797 g          669 t
ORIGIN      
        1 aagctgggag acggcggtgt ttctgcagtt attcctatgg aaaaagctga aggaggcacc
       61 tggaagaaag aggtttttgg cgatctagac ggccaaagat acaactacat tgtccggcac
      121 cacgacgtgg agtatgaggt ggtcgacccc tacgcccgcg cggtaacggt aaacggtcag
      181 atgggagttg tcgtggatct ttccaaaacc aacccaaagg ggtggaatga taccccagat
      241 cctgagctgc attcggcagt tgatgcaatt gtctacgaag ttcacgttcg ggacttctcc
      301 atttctcccg attcgggaat tcagaataaa gggctgtact tgggctttac ggagctaggt
      361 acaacgacct ccggttcggt ctataccgga gtaagtcatc tgaaggagct agggatcacc
      421 cacgtacagc ttctgcctat ctacgacttt gccacggtgg acgagacacg tcctttcgat
      481 tcgtacaact ggggctacga tcctctgaac ttcaacgctc cagaaggttc gtatgccaca
      541 gatcccaaag atcccatagc tagaattaga gagctgaagc agctcattca atcgttgcgc
      601 gaaaacggaa ttcgtgtggt gatggatgtg gtctacaacc acacttatta caccgaagag
      661 tctgcgttta ataaaatcgt acctgggtat tactaccgca cagacagaga tggggtctat
      721 tcaaacggct cgggctgcgg taacgaactg aagactgagc gcaagatggt gaggaagttc
      781 atcgtagatt ccatgtgcta ctgggcagag gagtacaaga tcaagggctt tagaatcgac
      841 cttatgggcg ttttcgacat cgagacggtg aacactctca gagaaaagct ccacaagatc
      901 gacccgtcca tcctcatcta cggtgaaggc tggaacggcg gcccgtctct tctcgacgac
      961 aacctgaggg cggtaaaggg gaaagcccat gagttcctct gcatcggagt gtttaacgat
     1021 gacttccgcg atgctatcaa aggcaatgtc ttccgtgcag aaaaccgggg gtttatctcc
     1081 ggtgcctacg gtatggagga gagcattaag tgcggcatca ccgcggctgt agatcaccct
     1141 ggtgttgact acaaaagagt gctgtactct agagcgccgt tttgcgtgaa cccctttcag
     1201 tccatcaact atgccgaagc tcacgacaat ctaacccttt tcgataagct gacccgttcc
     1261 aatcccgaag acgacgaacc gctgaccatc gccaggcaaa agctggcagg agcagtaatc
     1321 ctacttgccc aggggattcc tttccttcac gcaggacagg agttctgcag gaccaagttt
     1381 ggcgactaca acagctatag gtcgggggat ttcatcaacc agatcgtgtg ggcaaacaaa
     1441 gtgcggtatt acgatgtctt ccgctactat caggggctga ttgcgctgag aaaagctcat
     1501 cgggctttca ggctgcgcac tagagacgaa atcgtccggc atttggagtt tatgcctccg
     1561 cagggacctc tggtggtgaa gttccttctt aaggatcacg caggcggaga tccttgggag
     1621 aagattgtag tgtttttgaa cggggcaaga acggcgaagc aggtagttct tcccggagaa
     1681 gactgggtga tcgtagctga tgcaagaact gcaggcactg ccaagatcgg acaggttaaa
     1741 ggcagcgaag tgcttcttcc aagaatctcc tgtctggtgc ttgtagatcg caagagtttt
     1801 gaggaaggca aaaaagagtc ttgaggtgaa tctggtgaaa cacatctcct gcgtccatcc
     1861 aagtgaatac gatcgctacc tgttctgcga aggcagtcac tgcgaaagct acaggtttat
     1921 gggtgcacat ctttgtgagg aaaacggcac gccgggagtg cgctttacag tatgggcgcc
     1981 caatgccaaa agcgtgtcgg taattgggga tttcaacagt tggaatgaaa gcgcccaccc
     2041 catggagcgt ctcgaagaat cgggactttg gcaggctttc gttagaggag ctaaagaagg
     2101 cgacctttac aagtacgcag tcttcacctc cgatggacaa aagctgatca aggccgatcc
     2161 atatgccttc tactccgaac tccgtccgaa gacggcgtcg gttgtttggg attttaaaaa
     2221 gtacatatgg agcgacggtg actatctaag aaggcggagc tcgcaagcag tctacaaaga
     2281 accggtcaat atttatgaag tgcaccttgg atcatggaag caaaagccat tgggggactt
     2341 tctttcctac agagagcttg ctgatgagct tatcccgtac gcctacgaca tgggctacac
     2401 tcacatcgag attctgcctt tggcagagca ccccttcgat ggatcctggg ggtatcaggc
     2461 taccggctac tactctgcca ctagtcgcta cggaactcct gaggacctga tgtattttgt
     2521 ggacaaagca cacagctacg gtcttggtat catcctggac tgggttcctg gacacttctg
     2581 caaggacgag cacggcctta ggctctttga tggaactccg gcatttgaat acgacgagcc
     2641 ctggcgcagt gagaacctgg gctggggaac agccaacttc gacctaggga agggcgaagt
     2701 gcggagcttc ctcatctcca acgccgtttt ttggtttgac atcttccaca tcgacggtct
     2761 aagagtcgat gctgttgcca atatgcttta cctggacttc ggtagagaac cggggtcctg
     2821 gaaaccgaac atctacggag gaagggagaa cctcgaagct atcgagtttc ttagaaagct
     2881 caatgaagca gtgttcggaa gat
//
LOCUS       DAOSDA010000232         2895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22230_length_2895_cov_2.968310, whole genome shotgun sequence.
ACCESSION   DAOSDA010000232 DAOSDA010000000
VERSION     DAOSDA010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22230_length_2895_cov_2.968310"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /gene="deoA"
                     /locus_tag="PLW82_05900"
     CDS             <1..754
                     /gene="deoA"
                     /locus_tag="PLW82_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HOJ46842.1"
                     /translation="ESIPLIASSIMSKKIAGGAQGIVLDVKVGRGAFMKNIEEARKLA
                     ETMVALGDLVGRKVTAVLSDMDVPLSKAQGNTLEVIEAVNVLTLAETRSRRLQELSEV
                     LAAEMIRLDSSLQPSLDFSKAYERFASMVERQGGDLAKLKLDAPFKKQVTAKADGFFA
                     DADALAVGQIVRDLGAGRKVKGDPINPNVGVLFSKDLGERVAKGEVLATVYAQSQEDL
                     DRAVASLEEVLVVGPKPYDRPVVLDVIRGGDE"
     gene            754..1458
                     /locus_tag="PLW82_05905"
     CDS             754..1458
                     /locus_tag="PLW82_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46843.1"
                     /translation="MVWLLILLLAVNSFAAEHAVEIAGREYLVTSAFGGDILDGRYRQ
                     DLLEELRDSPYYGVIITDYSMEEHLERLKDLDLHDEMGTDDLERMGERIFLTFARKLY
                     QEGFDVAVLGQAGLDLVERGLVSFYFADDTVFYSNNLYYTLPLDFLPHLDWERTYAEE
                     VKHLREVALENSGDPFYTLTILALDSFGPDPEKQIVDFRESLAKANREGSALDRAWQR
                     LEEIWLQLKRQKLRNY"
     gene            1568..2818
                     /locus_tag="PLW82_05910"
     CDS             1568..2818
                     /locus_tag="PLW82_05910"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOJ46844.1"
                     /translation="MMAEQLTPMQIAQSQLKAAADKLDLEESVLEMLMEPERVVCVRI
                     PVKMDDGTTKIFNGYRSQHSTAIGPAKGGVRFHPGVNLDEVKALSFWMTFKCGVLGLP
                     YGGGKGGVQVDPTKLSRSELERLSRGYVRALADVFGADLDVPAPDVNTNGQIMAWMLD
                     EYAVINRKTDFAMLTGKPIVLGGSKGRVEATGRGAVVTLLEALKKLDMDIKGRTVAVQ
                     GFGNVGSAAADILADLGAKIVAISDISGGIYNENGIDIKALLEHRDKTGFVKDFSGAE
                     PMPGEKIFELPVDVIVPAALENVLTEENAHKVQAKIVLEIANGPTTPKADEIMREKGV
                     LVLPDILSNAGGVTVSYFEWVQNRYGYFWSEEEVNARLTQKMKESFANVWNMAKESSV
                     DLRTAAYMVSVKRIGEAIKLRGIV"
BASE COUNT          773 a          600 c          825 g          697 t
ORIGIN      
        1 cgagagcata ccccttattg catcaagcat tatgtccaaa aagatcgccg gcggggctca
       61 gggcatcgtt ctcgatgtga aggtaggccg gggtgccttc atgaaaaaca tcgaagaagc
      121 gaggaagctt gcagagacta tggtagcttt gggggatctg gtaggccgta aagtcactgc
      181 agttctttcg gacatggacg ttcccctatc taaagcccaa ggcaatactt tagaagtcat
      241 tgaagcggtg aatgttttga cgctagctga aacgaggagc aggcgtctcc aagaactctc
      301 tgaggttctt gcggcagaga tgattagact ggacagctcc ctgcaaccgt ctttagattt
      361 ttccaaagct tatgagcgtt ttgccagtat ggtggagcgc cagggtggag atcttgccaa
      421 attaaagctg gatgctccgt ttaaaaagca ggtcacagca aaggcagacg gctttttcgc
      481 cgatgccgat gcgctggcgg tagggcaaat cgtgcgcgac cttggcgctg gaagaaaggt
      541 taaaggagat ccgatcaatc ccaacgtggg agtcctgttc agcaaggatc tcggcgaaag
      601 ggtagcaaaa ggtgaggttt tggccacggt ctatgcgcaa agccaagagg atcttgacag
      661 agcggtggcc agtttggaag aagttttggt ggttggccct aaaccttacg ataggcctgt
      721 ggtgctggat gtgattagag gtggagatga gtaatggttt ggcttttgat acttcttctt
      781 gcggtgaact cttttgctgc agagcatgct gttgagatcg caggcaggga gtatttggtg
      841 acttctgcct ttggcggaga tatcctcgac ggacggtatc gccaggatct tctggaggag
      901 ctaagggata gtccgtacta cggggtgatc attacagact atagtatgga ggaacatcta
      961 gagaggctca aagatctgga tctacatgat gaaatgggaa cagatgattt ggagcgaatg
     1021 ggtgaacgca tcttcctaac ctttgcccgg aagctctatc aggaaggatt cgatgtggcg
     1081 gttttggggc aggcaggttt ggaccttgta gaaaggggac tggtctcctt ctactttgct
     1141 gacgacacag ttttttactc aaataacctg tactacactc ttccgttaga ctttctgccc
     1201 catctggact gggagagaac ttatgcagaa gaggttaagc accttagaga agtcgccctg
     1261 gaaaacagcg gagatccctt ctataccctc acaatcctag ccttagacag ctttggtccg
     1321 gacccggaaa aacagattgt agatttcaga gagagtttgg caaaagcaaa tagggaaggt
     1381 agtgctctcg acagggcttg gcagagactt gaagagatct ggctgcagct gaaacggcaa
     1441 aaactccgaa attactaatg tatacatgta tactagcagg tacggtgttg gacttcgtag
     1501 tttttgtgct aaaataagga ttaatacgtt ttttttagaa tgatttttgg acaatttcgg
     1561 aggtgcaatg atggcagagc agcttacccc tatgcagata gcccaaagtc agctcaaagc
     1621 tgcggcagat aaattagatc ttgaagaaag cgttctggag atgctcatgg agcccgaaag
     1681 agtggtttgc gtcagaatac cggttaagat ggacgacgga actaccaaaa tcttcaatgg
     1741 gtaccggtcc cagcactcaa ctgctatcgg gccagccaaa ggcggtgtca ggttccatcc
     1801 aggcgtaaac ctcgatgagg ttaaggcatt atcgttctgg atgacgttta aatgtggcgt
     1861 actcggactt ccgtacggtg gaggtaaggg tggtgttcag gtagacccta ccaaactctc
     1921 ccggagcgag cttgagcggc tcagccgtgg ctatgttcgg gctctggctg atgtttttgg
     1981 cgcggatttg gacgtcccag ccccagacgt aaacactaac ggacagatca tggcttggat
     2041 gcttgatgag tacgcggtga tcaaccgcaa aaccgatttt gccatgctta caggcaaacc
     2101 aattgttctc ggcggttcca aaggcagggt agaagctaca ggcagaggcg ctgtggtgac
     2161 gctcttggaa gcgctgaaga agttggatat ggatatcaaa ggccgaacag ttgctgtaca
     2221 gggctttggc aatgtgggca gcgctgcagc agacattttg gccgacttgg gggccaagat
     2281 cgttgccatc agcgatatca gcggaggaat ctacaacgaa aacggtatag acattaaagc
     2341 actgctcgag cacagggaca aaacaggatt tgtcaaggac tttagcggtg cagaacccat
     2401 gcctggcgaa aagatttttg aactaccggt agatgtgatt gttcctgctg ctttggagaa
     2461 cgtccttact gaagaaaacg cccacaaggt tcaagccaag attgttctgg agatagccaa
     2521 cggaccgaca acaccaaaag ccgacgaaat catgagagaa aaaggcgttc tggtgcttcc
     2581 ggatatcctg agcaacgctg gcggtgttac ggtgagctac ttcgaatggg tacaaaatag
     2641 gtacggctac ttctggagcg aggaagaagt caacgccaga ctcacgcaaa agatgaaaga
     2701 gtcgtttgca aacgtctgga acatggctaa ggaaagttca gttgatctgc ggactgcagc
     2761 ctacatggtt tcggttaaac ggatcggaga ggccattaaa cttagaggaa tagtataaaa
     2821 aaggagccaa ctggctcttt ttgtaagcgg caagtacttt tggataaatt tcggcagata
     2881 attttggaca cttct
//
LOCUS       DAOSDA010000233         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22272_length_2891_cov_3.253173, whole genome shotgun sequence.
ACCESSION   DAOSDA010000233 DAOSDA010000000
VERSION     DAOSDA010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22272_length_2891_cov_3.253173"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="PLW82_05915"
     CDS             complement(<1..142)
                     /locus_tag="PLW82_05915"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOJ46845.1"
                     /translation="MLVSAKEMLQKARKEGYAVGQFNINNLEWTKAVLQTAQEANSPV
                     ILG"
     gene            complement(373..804)
                     /locus_tag="PLW82_05920"
     CDS             complement(373..804)
                     /locus_tag="PLW82_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOJ46846.1"
                     /translation="MVRDKSDMKKLREAGYKLTAPREMVLDFLRDGRHYSAKEIYEGL
                     NKTLGLTTVYRTLELLLQVKLVKPLFLTDGSLKYEYVGSKDHHHHLICTDCGEILEIE
                     GCAVEGMEEQVQKKTRFKILHHTLEMYGVCPECQSSVDSKD"
     gene            complement(817..1308)
                     /locus_tag="PLW82_05925"
     CDS             complement(817..1308)
                     /locus_tag="PLW82_05925"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010899586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOJ46847.1"
                     /translation="MLSIVVAVGKNNIIGKENGLLWHLPNDLKHFRKITEGHTIIMGR
                     KTFESIGRVLPNRRHIVLTRSSDFQVEGVEKASSIEEVLKLVENEEEAFVIGGGEIYR
                     QFLPLVQRIYMTEVDIEKDADVFFPAIDSLEWKVVEEEPGVLDERNTIPHRFLILERV
                     ERS"
     gene            complement(1310..2101)
                     /locus_tag="PLW82_05930"
     CDS             complement(1310..2101)
                     /locus_tag="PLW82_05930"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008715044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOJ46848.1"
                     /translation="MSLADRQYLQIVEDVLAKGYFDQNRTGVPTYKLPHQIMQFDLEK
                     EFPILTTKFVAFKTAVKELLWIWQLQSNDVRVLQEMGCHIWDEWMLEDGTIGKAYGYQ
                     IKKYRQVDKLIETLKTNPQDRRMIVNLWNIEDLPEMALQPCAFLTLWDVTDGRLNCML
                     VQRSGDIGLGVPFNMTQYAVLVHLIAQVTDLRPGLFTHVINNAHIYENHVEALKLQLS
                     RREEALPAPKLIINPEIKDFYKFTPDDIKLDDYKHLGKIEMEVAV"
     gene            complement(2163..>2891)
                     /locus_tag="PLW82_05935"
     CDS             complement(2163..>2891)
                     /locus_tag="PLW82_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOJ46849.1"
                     /translation="VLKGLSFAGAGIAHGAFAGVAFAFLIGANPFMMGLLFALAMVLM
                     VGVSSKKGNLNVDASIGILFALTMALAILFIGMMKQYNTELFSFLFGNILAVTPEDLH
                     FMSFVFLGVVVVLLIGYRAFYFWAFDEVMAEASGVLMPQVFYLFLAMLALAIVASLKV
                     VGELLVNAFIIIPAASSFQLTYSFKRMALYSVLLGAFASVTGLILSFIYDIPSGASIV
                     IILGLEFLVCTVLSPKKRWHKGKQ"
BASE COUNT          721 a          754 c          643 g          773 t
ORIGIN      
        1 cgcctaagat aacgggagaa ttggcttcct gtgccgtctg caagacagct ttagtccatt
       61 cgaggttgtt gatattgaac tggcctactg catagccttc tttccttgcc ttttgcagca
      121 tttcctttgc tgaaactaac actaaaatcc ctccaaaatc taacagcttt gtgctgtttt
      181 aattataacg tagttagaag caaaatgcag aagtattttt gctttctcga gcttgggcat
      241 gccttttgat gggccttcag ggaacacccc ttagttctgc cctgcgttta cctttggact
      301 tgtcgcagaa gtccaccagg ccagtgctgc tgaagcaccc cagagaaaaa gagctgtttg
      361 ccaacaactc ttttagtctt tgctatccac tgaactctgg cattcggggc aaacgccgta
      421 catctcaagg gtgtggtgca ggattttaaa ccgtgttttt ttctgtacct gctcttccat
      481 tccctctaca gcacagccct cgatctccag aatctcaccg cagtctgtac agatcaagtg
      541 gtgatggtgg tctttgctgc ccacatactc gtacttgagc gaaccgtcgg taagaaacag
      601 gggtttcact aatttcacct ggagaagcag ctccaatgtg cggtagactg ttgtcaaccc
      661 gagagtcttg ttcaatcctt catagatctc ttttgccgag tagtgacggc catctctgag
      721 aaagtccaag accatctctc gaggtgctgt aagcttatag cctgcttccc tcagcttttt
      781 catatcgctt ttatctctta ccactaaaga ccaccttcag ctgcgctcta cgcgctctag
      841 aatgaggaac ctatggggaa tggtgtttct ctcgtccagt acgcccggct cctcctccac
      901 taccttccac tctaagctgt cgattgcagg aaagaaaacg tccgcatctt tctctatgtc
      961 cacctctgtc atgtaaatgc gctgaaccag aggtagaaac tgccggtaaa tctctcctcc
     1021 gccgatgaca aacgcttcct cttcgttttc cacaagcttc agcacttctt caatggaaga
     1081 ggctttttcc actccttcca cctggaagtc tgaacttcgt gtcagtacaa tgtgccttct
     1141 gtttggcaga actctgccga tgctctcaaa ggtctttctg cccatgatga tggtgtgccc
     1201 ttcagtgatc tttctgaagt gcttaagatc gtttggcagg tgccagagga gcccgttctc
     1261 tttgccgata atgttgtttt taccgacagc taccacgatg ctcagcatgc tacactgcga
     1321 cctccatctc tatcttgccc agatgcttgt aatcatccag cttgatgtca tcgggagtga
     1381 acttgtagaa gtccttgatc tcgggattga taatcagctt tggtgcagga agggcttctt
     1441 ctctgcggga aagctgaagc tttaacgctt ccacatgatt ctcatagatg tgggcgttgt
     1501 tgattacgtg agtaaaaaga cctggacgca gatccgttac ctgcgcgatc aggtgtacca
     1561 gcactgcata ctgtgtcatg ttgaacggta cgcctaggcc gatgtcgcca ctccgttgga
     1621 ctagcatgca gtttaaccta ccgtctgtta catcccaaag tgtcaggaaa gcacagggct
     1681 gtagtgccat ttcagggaga tcttcaatgt tccacaggtt gacgatcatt ctgcggtcct
     1741 gaggattggt cttgagcgtc tcaatgagct tgtccacctg gcgatacttt ttgatttgat
     1801 agccgtaggc tttgccgatg gtgccgtctt caagcatcca ttcatcccaa atgtggcatc
     1861 ccatctcctg caagactctg acgtcgttac tctgcagctg ccagatccaa aggagttcct
     1921 tcactgcggt cttgaaggct acaaacttcg tggtcaagat gggaaactct ttttccagat
     1981 caaactgcat gatttgatgc ggaagcttgt atgtgggcac gccggtgcgg ttttggtcga
     2041 agtagccctt ggccaaaaca tcctcgacta tttgcaaata ctgtctgtct gctaagctca
     2101 ttgcagttcc cccttcatct ttcgttccta atactattac ccaaatactt gcccaaaacc
     2161 tcttattgtt ttcctttgtg ccaccgcttc ttgggcgaca gtacggtaca gactaaaaac
     2221 tccaaaccga gaataatcac gatgctggct cctgaaggga tgtcgtagat gaaagacagg
     2281 atcaaacctg tgactgaagc aaaagctccc aaaagcacgc tgtacagtgc cattcttttg
     2341 aaagagtagg tcagctggaa actgctcgca gcgggaatga tgatgaaagc gttgaccaga
     2401 agctcgccta caacctttaa agacgccacg atagcaaggg caagcatagc caaaaacagg
     2461 tagaagactt ggggcattag gactcctgaa gcctctgcca taacctcatc gaaagcccag
     2521 aagtaaaaag ccctgtagcc aataagcaaa accactacaa caccgaggaa aacaaaactc
     2581 ataaagtgca gatcttctgg agttaccgcg aggatattgc cgaacagaaa gctgaaaagc
     2641 tcagtattgt actgcttcat cataccgata aacaagattg ccagagccat ggtcagagca
     2701 aagagaatgc caattgatgc atccacattc agattgccct tcttggaaga gacgccgacc
     2761 atcaaaacca ttgccagggc aaaaagcaac cccatcataa acggatttgc cccgatgaga
     2821 aatgcaaacg cgactccggc aaaggcgccg tgggcgattc ccgctccggc aaacgacagc
     2881 cctttcaaga c
//
LOCUS       DAOSDA010000234         2888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22304_length_2888_cov_2.943523, whole genome shotgun sequence.
ACCESSION   DAOSDA010000234 DAOSDA010000000
VERSION     DAOSDA010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22304_length_2888_cov_2.943523"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLW82_05940"
     CDS             <1..357
                     /locus_tag="PLW82_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOJ46850.1"
                     /translation="VLIVDTAGRLHTKSNLMDELKKVRRVLEREMPGAPHDVLLVIDA
                     TTGQNGLNQAKVFGEATGVSGIVLTKLDGTSKGGIVVAIKKELGLPVKFIGVGEGIDD
                     LQEFDPNEFVEALFED"
     gene            425..769
                     /locus_tag="PLW82_05945"
     CDS             425..769
                     /locus_tag="PLW82_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HOJ46851.1"
                     /translation="MEIVKTVRMGLLNDFYGQLLTPKQQRCIQLYYEADLSLAETAER
                     FGSTRQAVYDLLKRTERLLEEYESKLGLLEKHEKRMQKYRRIRELAVGSQHEKEIREI
                     VAELERMETGDF"
     gene            759..2096
                     /gene="ffh"
                     /locus_tag="PLW82_05950"
     CDS             759..2096
                     /gene="ffh"
                     /locus_tag="PLW82_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOJ46852.1"
                     /translation="MIFENLAEKLQNTFSSLKNRGKLSEKDVEAALKEVRLALLEADV
                     NYKVVKTFTEKVRSRAVGEEVLKSLTPAQQVIKIVYDELTALMGGSVSSLDLSHSPSV
                     IMMVGLQGAGKTTTAGKLAKSIKERGRRPLLVACDIYRPAAIKQLQVVGSQVGVEVFS
                     LEKEKPVAIAKAALKHAALNGFDVIIIDTAGRLHIDEELMNELVEIKGTVKPHEILFI
                     ADAMTGQDAVNVADSFNQRLDITGVILTKMDGDARGGAALSIREVVGKPIKYASFGEK
                     FDSLEPFHPDRMASRILGMGDLGTILEKAQQAIDEKEAQKMMDKLTKDSFNLEDFYEQ
                     LQQMKKMGPLNQLLEMVPGMGKQLKGQDLSGAEGEMKRIEAIINSMTVKERRQPEIID
                     ASRKRRIANGAGVKVQDVNKLLKQFGETKKMMKHFSGLATGKGKRKIKMPFFG"
     gene            2114..2383
                     /gene="rpsP"
                     /locus_tag="PLW82_05955"
                     /pseudo
     CDS             2114..2383
                     /gene="rpsP"
                     /locus_tag="PLW82_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004907956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            2426..2656
                     /locus_tag="PLW82_05960"
     CDS             2426..2656
                     /locus_tag="PLW82_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOJ46853.1"
                     /translation="MKELVEVLAKSLVDNPDQVLVTEKLEGDSIILELKVADSDMGKV
                     IGKRGRIAKALRTVLKAAANKDKQKVMLEIVG"
     gene            2704..>2888
                     /locus_tag="PLW82_05965"
     CDS             2704..>2888
                     /locus_tag="PLW82_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HOJ46854.1"
                     /translation="MRLYTIGKIVSTHGLKGEVKVYPETSFPERFLEMERVLLGDESY
                     QDVYVIESARIQKNAVI"
BASE COUNT          856 a          615 c          792 g          625 t
ORIGIN      
        1 gttctcatcg tggacacggc aggacggctc cacaccaaga gtaacttgat ggatgagctg
       61 aaaaaagtcc gcagggtttt ggagagagag atgccagggg ctcctcacga cgtgctgctg
      121 gtgatcgatg ccacaacagg ccaaaacggt ctaaaccaag cgaaggtctt tggcgaggcc
      181 acaggagtaa gcggtattgt gctcaccaaa cttgacggaa catctaaagg tggtatcgtt
      241 gtggccatca aaaaagagct gggacttcct gtgaagttca tcggtgtcgg ggagggcatc
      301 gatgacctac aagagttcga tccgaacgag tttgtagagg cgctatttga agattaaagg
      361 tgtaaagaaa aaaacttgac accgttaggg agataattta tactacctaa ggtaggggac
      421 ggccatggaa atagttaaaa cggtacgcat gggtcttctc aatgattttt acgggcagct
      481 gttgacgccc aagcagcagc gctgcatcca gctttactat gaagccgatc tctcgcttgc
      541 cgagactgcc gaacgtttcg gcagtaccag acaggcagtg tatgatctcc tcaagcgtac
      601 agaaaggctc cttgaagagt acgaaagcaa gctgggcctt ctggaaaagc acgaaaagcg
      661 catgcagaag tacagaagga tcagggaact tgccgtaggt tctcagcatg agaaggagat
      721 tagggagata gtagctgaac tcgaaaggat ggagaccggt gatttttgaa aacctagcgg
      781 agaagctgca gaacacattc agcagcttaa aaaaccgcgg taagctgagc gaaaaagatg
      841 tagaggcggc tttgaaagag gtccgtttgg ctctacttga ggcagacgtt aactataaag
      901 tagtaaaaac ctttacagaa aaagtcagaa gcagagctgt tggtgaagag gtcttaaaaa
      961 gccttacccc tgcacagcag gtgatcaaga tcgtctacga cgaactcaca gctctgatgg
     1021 gagggagcgt ttcctcgctc gatttgtccc actcgccgtc ggtgatcatg atggtgggtc
     1081 tccaaggtgc tggtaagacc acgacagctg ggaaactggc caagagcatc aaggagagag
     1141 gacgccgtcc gctgcttgtt gcctgcgaca tctacagacc agctgccatt aaacagctgc
     1201 aggtcgtcgg gtctcaggta ggggttgagg tgttcagcct tgagaaggag aaacccgttg
     1261 cgatagccaa agcagcgctg aagcatgcag ctctaaacgg ctttgatgtg atcatcatcg
     1321 atactgccgg taggcttcac atcgatgaag aactcatgaa tgagctggta gagattaagg
     1381 gaacggtcaa gccccatgag atcctgttta tcgccgatgc catgacaggg caggatgcag
     1441 tgaacgttgc agacagcttc aaccagcgtc tggatatcac cggcgttatc ctaaccaaaa
     1501 tggacggaga tgccagaggt ggcgcagcgc tatccatccg cgaggtagta ggcaagccca
     1561 taaagtacgc atcttttggc gaaaagttcg attctttgga accgttccat cccgaccgga
     1621 tggcgtcgcg aattctgggt atgggcgatc tgggaaccat cctggagaaa gctcaacaag
     1681 ctatcgatga aaaagaagcc cagaagatga tggacaaact gactaaagac agcttcaacc
     1741 tggaagactt ctacgaacag ttgcagcaga tgaagaaaat gggtccgctc aatcagcttc
     1801 tggagatggt gcccggtatg ggcaagcagc tcaaggggca ggacttaagc ggtgctgaag
     1861 gtgaaatgaa gcgcattgaa gctatcatca actcgatgac agttaaagaa cgaagacaac
     1921 ctgagattat tgacgcctcc cggaagcgca ggattgccaa cggagctggc gttaaagttc
     1981 aggatgtaaa taaactgctc aagcagttcg gcgaaaccaa aaaaatgatg aagcatttta
     2041 gcggtctcgc aactggtaaa ggcaaacgca aaataaaaat gccgtttttc ggttaaatag
     2101 gaggtgaaat gctgtgagtg taagaatcag actgcgccgt atgggcaaga agaaacaacc
     2161 attctataga ctggtagtag ccgactctcg cacacaaaga gatggtcgtt ttatcgatat
     2221 tattgggacc tataaccctt tgacccagcc tgctgagatc aacattgatg aagaaaaagc
     2281 tttggactgg ctgcaaaaag gtgctcagcc atctcagaca gcaaagagac tgctatctca
     2341 agctggagtc attgccaagc acgccgacaa gggtgccaaa gaggacaagt aggtttttag
     2401 ttcaactgtt aaggaggcct gtgccatgaa agaactcgtt gaagttcttg ccaaaagcct
     2461 tgttgataac ccagaccagg ttcttgttac tgagaaactc gaaggcgact ccattattct
     2521 cgagcttaaa gtagcagata gcgatatggg aaaggttatt ggtaaacggg gtagaatagc
     2581 taaagccctg cgaacagtgc tcaaggccgc agccaataaa gacaaacaga aagtcatgct
     2641 tgaaattgtg ggataaagtt cagcaggagc aggagctcct gctttttttc ggaggtccgt
     2701 tagatgaggc tttataccat aggtaagatt gtcagtactc acggccttaa aggtgaggtt
     2761 aaagtatatc ctgaaacgtc ctttcccgaa agattcttgg agatggaaag agttctcctc
     2821 ggcgacgaat cctaccagga tgtctatgtc atcgaatctg ccaggatcca gaagaacgca
     2881 gtgatttt
//
LOCUS       DAOSDA010000235         2886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22322_length_2886_cov_3.081950, whole genome shotgun sequence.
ACCESSION   DAOSDA010000235 DAOSDA010000000
VERSION     DAOSDA010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22322_length_2886_cov_3.081950"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PLW82_05970"
     CDS             <1..495
                     /locus_tag="PLW82_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46855.1"
                     /translation="QLELYANLFELAVVLGGFGQRSRRGYGSLQIEGVTWSSKDEYIG
                     YVEERLAKLAKKEKCIRTIKSDLPDSKYPVLRNIWIGKAEDDPETILRMIGSETSRLK
                     AEFKEGYALGSPGSKPASPLHVSIHQFTFGYCPVIVEVFKERSPNPRYSQQRGRLLQA
                     LGVK"
     gene            497..2263
                     /gene="cas10"
                     /locus_tag="PLW82_05975"
     CDS             497..2263
                     /gene="cas10"
                     /locus_tag="PLW82_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HOJ46856.1"
                     /translation="MSKRDYLLLFSIGPVQSFIAAARKTEDLWAGSYLLSFLVEKAID
                     KLEETVSENGGDLELIYPVKLKGADKAYGSMVASYPNRFLSLVSTDSEKLPAICETLA
                     DFIYGQFEEMCLFGLEDAFRMRGNGDSLMRDLTTHQARELLEVFWAVEPIESGDYEGA
                     RKNVESRLAAIKNNRTFSLQPQDGIVCTVCGEREALHETPYGENDSVGKMRSELRRTW
                     SKLSGKYRSKEERDEAFDGTAFYTGRIRENEALCGVCLGKRTLRDYMSERGKRSIHGF
                     RSTVEISAPLNYYAIFTMDGDDMGRWLAGKEGRFSEDPGTIGFHRLISEKLAKFSGEA
                     VPRRVLEAGGELVYAGGDDVLCFVPVPGLFQLINNLRLDFQNSEHGLHPEATASAGVV
                     IVHKQYPLSSALNHARAMACRAKQYKHPVTGKSKDALGVAVFTRSGVINDFVVPWMLA
                     GPFVTDSLDNVPTLMEHLVGLMKDDLSSSFIEKLGEAFLPLIGSDTKREVKIKLAEDP
                     AESTKALSVEMNRLLGRSLIDRESQFASKLPEYADYLAKVHDVMGSGHEFLNWLQALR
                     ALARLLKENKEVQSDEAGTNAN"
     gene            2238..>2886
                     /locus_tag="PLW82_05980"
     CDS             2238..>2886
                     /locus_tag="PLW82_05980"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HOJ46857.1"
                     /translation="MKLVLTPTDTFFFRNHKAMNAGADFFASGLFPPRPGTVYGALRS
                     AYIHAKSDFTAFAAGSDEELSKWMGTPSSYGKFAIKGVVLQDEQDMALPLPLDHQVVL
                     GISGSATAKRLRLKYEPEKEVFGSDRCPYRLYGVSEEKSASASGRFVYRQDFVKALLQ
                     PVDLEVHSASRWVISEQRVGIAMDRVAKKAQQSMFYSMEALRFRDENGTAIAAICD"
BASE COUNT          766 a          673 c          772 g          675 t
ORIGIN      
        1 cagctggagc tgtacgccaa tctcttcgag ctagctgtag tcttgggtgg atttggacag
       61 cgttctcgta gaggctacgg gtctttgcag atcgagggcg ttacgtggtc ttcaaaagat
      121 gagtatatcg gttatgtaga agaacgcctt gcaaagctag caaagaaaga aaaatgcatc
      181 aggacgataa aatctgatct tcccgattcg aaatatcccg tgctgcgaaa catctggatc
      241 gggaaagcag aagacgatcc cgagaccatc ctcagaatga tcggcagtga aacaagcagg
      301 ttaaaagctg agtttaagga ggggtatgcc ttgggcagcc caggttcgaa accagcgtcc
      361 ccattgcacg tgagcatcca ccagttcacc tttggttact gcccggtcat tgtggaagtt
      421 ttcaaagagc gcagccctaa tcctcgctac tcacagcaaa ggggcaggct attgcaagca
      481 ttgggggtga aatagggtgt ctaaacgtga ttacttactc ttgttcagta taggacctgt
      541 ccagtctttt attgcggccg ctcgtaaaac agaagacttg tgggcaggaa gctatctcct
      601 gtcttttctg gtggaaaagg ccatcgataa actcgaggaa accgttagtg aaaacggtgg
      661 cgatttagag ctgatttatc cggtgaagtt gaaaggtgca gacaaagcct acggctcgat
      721 ggtagcttcc tatcccaatc gcttcctctc gctagtgtca acggactcgg aaaagctccc
      781 ggctatctgc gaaactctag cagacttcat ctatggacag tttgaggaga tgtgcctctt
      841 cggtcttgaa gatgctttcc ggatgcgggg aaatggcgat tctctaatgc gcgacctgac
      901 cacacaccaa gctagggagc tgttggaagt gttttgggct gtagaaccca tcgaatccgg
      961 cgattacgaa ggagcgagga aaaacgtcga gagccgtctg gcagcgatta aaaataaccg
     1021 tactttttcg ctgcagcctc aggacggaat cgtgtgcacg gtttgtgggg aaagggaagc
     1081 cctgcacgaa acgccgtacg gtgaaaacga ctctgttggt aaaatgcgca gtgaacttcg
     1141 acgaacttgg agcaaactca gcggcaagta ccggagcaaa gaagaaagag atgaagcctt
     1201 tgacggcaca gcattttata caggacgcat acgggaaaac gaagcgctct gcggagtctg
     1261 cctcggaaag cgcactctgc gggactacat gtctgaacgt ggtaagagaa gcatacacgg
     1321 ctttcgctcc actgtcgaga tctcggcccc gctcaactac tacgcgatct tcaccatgga
     1381 cggcgatgac atgggaagat ggcttgcagg aaaagaagga aggttttcag aagatccggg
     1441 aaccatcggc ttccatcgat tgataagtga aaagctggca aagttttctg gtgaagctgt
     1501 tccgagaaga gtattggaag ctggtgggga gctggtgtat gccggaggcg atgacgtttt
     1561 atgcttcgta cctgttcccg gactgtttca gctaattaac aacttgcgct tggacttcca
     1621 gaactcggaa cacggcctac atcctgaagc aacagcatct gccggagtag taatcgtaca
     1681 taaacaatat ccgttaagtt ccgcactaaa ccatgctcga gctatggcat gccgcgccaa
     1741 gcagtacaag catccggtaa ctggcaagag caaagatgca ctcggtgtgg ctgtgttcac
     1801 cagaagtggc gtaatcaacg actttgttgt tccctggatg cttgcaggtc cttttgtcac
     1861 cgacagctta gacaatgtac ccaccctcat ggaacatcta gtcggcttga tgaaagacga
     1921 cctatccagt tcgttcattg agaagctcgg cgaagcgttt ctccctctta tcggttctga
     1981 caccaagaga gaggtaaaga tcaagcttgc cgaagaccca gcagaaagta caaaggctct
     2041 ttcggttgaa atgaacagat tgcttggtcg ctccctaatt gatcgggaat cgcaatttgc
     2101 gtcaaaactt cccgaatatg cagactatct tgccaaagtc cacgacgtaa tgggatctgg
     2161 acacgagttt ctcaattggc tgcaagccct ccgggcatta gcaaggctgc tgaaagaaaa
     2221 caaggaggtg caaagcgatg aagctggtac taacgccaac tgacacgttc ttctttcgca
     2281 atcacaaagc gatgaatgca ggcgcggatt tttttgccag cggacttttc cctcctagac
     2341 ccggcactgt gtacggagcc ctccgaagtg cttacatcca cgcaaagagc gactttaccg
     2401 catttgctgc aggtagtgat gaggaactct ctaagtggat gggcactccc tcaagctacg
     2461 gcaagtttgc cataaaaggt gtagtcctcc aagacgagca ggatatggcc ttgcccttac
     2521 ctctggacca tcaggtcgtc ctgggaatat cggggtctgc tactgcaaaa cgcttacggc
     2581 tcaagtatga gccagagaaa gaggttttcg gatcagatcg ttgtccctat cgtctttatg
     2641 gggtttcgga ggagaaatcg gcttcagctt caggacgttt tgtctacaga caggacttcg
     2701 ttaaagcatt gctgcaacct gtggacctgg aagtccattc tgcaagtagg tgggttatta
     2761 gcgaacaaag agtaggaata gccatggacc gagtggcgaa aaaagctcaa caaagcatgt
     2821 tctactctat ggaagcactg cgttttaggg acgagaatgg cactgctatc gctgcaatct
     2881 gcgata
//
LOCUS       DAOSDA010000236         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22418_length_2877_cov_3.390858, whole genome shotgun sequence.
ACCESSION   DAOSDA010000236 DAOSDA010000000
VERSION     DAOSDA010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22418_length_2877_cov_3.390858"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>466)
                     /locus_tag="PLW82_05985"
     CDS             complement(<1..>466)
                     /locus_tag="PLW82_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOJ46858.1"
                     /translation="GSRHPVSNALMMAPVFVEVNNTAIQPYINEEIGFQEATQNALKP
                     IREYMRNQTRKSDLDLFMQMHGVASVPTVEDVPDYVLMPAYLISELKTAFQMGFVLFI
                     PFLVIDMVVASILMSMGMMMLPPVMISLPFKILLFVMVDGWNIITRSLVLSF"
     assembly_gap    467..476
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<477..798)
                     /locus_tag="PLW82_05990"
     CDS             complement(<477..798)
                     /locus_tag="PLW82_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HOJ46859.1"
                     /translation="MTLTKKSLVLLLVLIFLALPSVAFAEETLLPSLNISIGEAEGPQ
                     EVASTLTLVLLLTILSLVPSILILATAFTRIVIVLSLLRNAMGLQQIPPNQVIIGLAL
                     FLTIF"
     gene            complement(795..1100)
                     /locus_tag="PLW82_05995"
     CDS             complement(795..1100)
                     /locus_tag="PLW82_05995"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOJ46860.1"
                     /translation="MDLTYLLKWLGATLGVLGMIYLLYFLAGKKIAKKGRDLEIVEAL
                     PLGNRLIIYLVRVKDRILAVGVSPAGMHVLTQFDPGDTEASDGFDVESEEMAGDGER"
     gene            complement(1113..2180)
                     /gene="fliN"
                     /locus_tag="PLW82_06000"
     CDS             complement(1113..2180)
                     /gene="fliN"
                     /locus_tag="PLW82_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOJ46861.1"
                     /translation="MSDHLLSQSEIDALVKAGAKKGKSPAKQEVRPPAEVVKKLFTAL
                     EPKLGEALGLLTANRAVTFAAECYVKKGAEVDDKRYVISLGSTEEQVEVLIFVDEQAA
                     SSILKKISEEPERTEEYFTASNMEVFGKVIEVLADEFSAQTSDLAERHISLSVALPKG
                     LSSLQEFLDSKAEYVVLKYNLEWNEDEVGMLGFVMKNDDVPLILPDEDQQETVSSIDD
                     LIEDSNNGQDEDIFAHLMNELSSEEEPAEEVVVKPVQFGNLKGGSKQPVKGEDNLEII
                     LDLPVMVTVELGRTKKSLEEILNLKPGSVVELDRLAGEPVDVLANGVLIAKGEVVVID
                     ETFGVKITEIASRKQRINRIK"
     gene            complement(2173..>2877)
                     /locus_tag="PLW82_06005"
     CDS             complement(2173..>2877)
                     /locus_tag="PLW82_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HOJ46862.1"
                     /translation="PNPTLMATISLDPLPGYMIWEINPDISICIVERLLGGYGDSKDH
                     IRQLTEIEVTVIRNMIDKLTPDVHNAWINVVSLRPELVNLESNPMYSHAAPPTEMVIA
                     VTMEMSLLNRVGKMNICLPYTMLEQVMSRLSAQFWFATDKTGSDESAMRKLKDLLRPT
                     EFELVLELGKAQITVGQLLEMEVGDVLTLESQIGDPLEVKLNGKTKFLGRPGLVGKKL
                     GVEILDVVSDGGKQDE"
BASE COUNT          682 a          766 c          677 g          742 t
ORIGIN      
        1 ctaaagctca aaacaagtga gcgggtaata atgttccagc catcgaccat gacaaacaga
       61 agaatcttaa acggcagaga tatcataaca ggaggtagca tcatcattcc catggacatg
      121 agaatgctag ctacaaccat gtcgataact aagaagggta taaagagaac aaagcccatt
      181 tgaaaagcgg ttttcagctc gctgatcaag tacgcgggca taagcacgta gtccggcacg
      241 tcttctacag tgggaacact cgccacaccg tgcatctgca taaacaggtc cagatcgctt
      301 ttgcgcgtct ggttgcgcat atactctctg atgggcttaa gggcattttg cgtggcctct
      361 tgaaagccta tctcctcatt gatgtagggc tgaatggcgg tgttgttgac ctccacgaac
      421 accggtgcca tcatcaaagc attcgacacg ggatggcggg atccttnnnn nnnnnncgaa
      481 aatcgtgaga aaaagcgcaa ggccgataat cacctgattg ggaggtatct gttggagtcc
      541 catggcattg cgcagaagcg acaggacaat cacgatccgg gtgaacgccg tagccagtat
      601 cagtattgaa ggtacgagag acagaatcgt gaggagcaga acgagcgtca gcgtagacgc
      661 cacctcttgg ggaccttccg cttcgcctat ggagatgttt aaagatggta gcaacgtttc
      721 ttcggcaaat gcgacgctag gcagtgccaa aaaaatcaaa accaaaagca gtaccaagct
      781 cttttttgtc agtgtcatct ttctccgtca ccagccattt cctctgactc gacgtcaaat
      841 ccgtctgagg cttcagtgtc tccaggatcg aactgcgtaa gcacatgcat tccggcaggc
      901 gatactccta cagcgagtat ccggtcttta acccggacaa ggtagataat cagcctgttt
      961 ccgagaggca gagcctcaac gatctcaaga tcgcggcctt tttttgcgat ctttttccct
     1021 gccaaaaagt aaagcaggta gatcatacca agcacgccga gtgttgcccc aagccacttc
     1081 aaaagatacg tcaggtccac cagtatcccc tcttacttaa ttctgtttat ccgctgcttc
     1141 ctgctggcaa tctccgtgat cttcacgccg aaagtctcgt caataacgac aacctcgccc
     1201 ttggcgatga gcactccgtt tgccagaaca tctaccggct caccggctag gcgatcgagc
     1261 tccactacgg atccaggctt cagattgagg atctcttcca ggctcttctt ggttcttccc
     1321 agttcaacgg tgaccatgac cggcaagtcc aaaatgatct ccaagttgtc ttcccctttg
     1381 acaggttgct tacttccgcc tttgagattg ccgaactgta ctggctttac cacaacctct
     1441 tcagctggct cttcttcgct tgataactca ttcataaggt gagcaaagat gtcttcatcc
     1501 tgcccgttgt tgctgtcttc gatcagatcg tcgatgctag aaaccgtttc ttgttggtct
     1561 tcatcgggaa ggatcagcgg gacatcgtcg tttttcatga caaaaccaag catacctacc
     1621 tcatcctcat tccattcaag attgtatttt agtacgacgt actctgcttt ggaatccaag
     1681 aactcctgca ggctgctgag acctttaggt agagctacag aaagggagat gtgccgttct
     1741 gcaaggtccg atgtctgcgc ggaaaactcg tctgccaaca cttcgatgac cttgccgaag
     1801 acctccatat tgctagctgt aaagtactct tcagtacgct cgggctcttc ggatatcttt
     1861 ttgagaatgc tggaagctgc ctgttcatct acaaagatca gcacttcaac ctgctcttct
     1921 gtactaccta ggctgatcac atacctcttg tcgtcaactt cagcgccttt tttgacgtag
     1981 cactcggctg caaaggtgac ggcacgattg gctgtaagca ggccaagggc ttccccgagt
     2041 ttgggctcta gtgcagtaaa gagcttcttc actacttctg caggaggtcg cacttcctgt
     2101 tttgccgggc tcttgccttt tttcgctcct gcctttacca gagcatctat ttcgctttga
     2161 gataaaaggt gatcactcat cttgcttacc tccatctgaa actacatcca gtatctccac
     2221 accgagcttc ttgcccacaa ggcccggccg gcccaaaaac ttggtttttc cgttcagctt
     2281 gacctccaga ggatcgccga tctgcgactc caaagtcaat acgtcgccca cttccatctc
     2341 caagagctga cccactgtga tctgcgcttt gccgagctcg agtacgagtt cgaactcggt
     2401 aggccttaat aggtctttga gcttgcgcat tgccgactcg tcgcttccgg tcttgtcggt
     2461 ggcaaaccag aactgcgcgc tcagcctgga catgacctgc tcgagcatgg tgtagggcaa
     2521 gcagatgttc atcttaccta ccctgttgag aaggctcatc tccattgtaa cggcaatcac
     2581 catctccgta ggcggagctg cgtgactgta catggggttg gactcaaggt taaccagctc
     2641 aggtcttaga ctcaccacgt tgatccaagc gttgtgaaca tcgggggtga gtttgtcgat
     2701 catgtttctg atcaccgtga cttcgatctc ggtgagctgg cggatgtgat ctttagaatc
     2761 cccgtatcca cctaaaagcc tttcaactat gcagatgctg atatcagggt tgatctccca
     2821 gatcatgtat cccggaaggg gatccagaga gatggtggcc atgagtgtgg gattggg
//
LOCUS       DAOSDA010000237         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22610_length_2859_cov_3.101997, whole genome shotgun sequence.
ACCESSION   DAOSDA010000237 DAOSDA010000000
VERSION     DAOSDA010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22610_length_2859_cov_3.101997"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PLW82_06010"
     CDS             complement(<1..815)
                     /locus_tag="PLW82_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOJ46863.1"
                     /translation="MHMALRSHGRYGFIKPSLDAHTLGINSAAELLRSCGYEVLIAEG
                     PVEQAILDYSYESRREVVINWLVENRISRVGISYRLDEADAVNMVGYLVNEMQRRSLF
                     SYQGGFIEAVYFAGLPRTCTLIEREFGGLVKTFSGGESPYDTLLKLGVPGESIPKTIS
                     WASKYDDLLMEFGREVVSSGEHVTVKPPKRPTYLEMGTKQDTVVKRLDANLKQAEPFY
                     PLVRAHLGPYSAELKREEAVRQFVSWAEMRAKSGYLDILSIGTSQLTQSNFGE"
     gene            complement(799..1890)
                     /locus_tag="PLW82_06015"
     CDS             complement(799..1890)
                     /locus_tag="PLW82_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOJ46864.1"
                     /translation="MNVTVLGAGNSGLAMAAHLSLYGNKVRLWNRSKENIAKIMETGT
                     IYCEGVICGNGKINLVTSDIALALEGTDLVLVTTPANSHRDLAKLVAENLKESVLIVL
                     NPGRTFGAIEFKKVFSAYNTRSEPLVAETQTTIYTCRKTGEDSIHVIALKDSVLLSAL
                     DASLNESIIQMLPECIRRYFVPAKSLVETSIGNVGMILHCAPLLLNAGWTENKDNIYK
                     YYYDGITPTIAQFLEEIDRERITVSEKLGLRVESTQDWLKRTYGVKGRNLYECIQNND
                     AYKHIEAPSSLQHRYIYEDIPFGLVPLESIGHTLGLSMSNTTLIIDLACRLLGVDFRR
                     EGRTLDYLSENSDVLTYLSPAERSDAHGA"
     gene            complement(2611..>2859)
                     /locus_tag="PLW82_06020"
     CDS             complement(2611..>2859)
                     /locus_tag="PLW82_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46865.1"
                     /translation="QKHALNVPFLVVRGVSDHADDAIDLKEPLVYQDTKALQKELTAV
                     NADPMQKASEAAKSLHSVFQKAAYNASTVVWSVIDKLS"
BASE COUNT          684 a          714 c          650 g          811 t
ORIGIN      
        1 tcctcgccga agttggattg agtgagctgg gatgtgccta tggaaaggat atccaaatag
       61 ccgctttttg cccgcatctc ggcccacgaa acgaactgcc gtaccgcttc ttccctcttc
      121 agttcagcgg agtacggtcc cagatgtgcc ctgaccagcg gatagaacgg ctccgcttgt
      181 ttgagatttg catcaagtcg ctttaccacg gtgtcctgct ttgttcccat ctcaaggtag
      241 gtaggtcttt taggcggttt gactgtcacg tgttctccgg agctgaccac ctcccggccg
      301 aactccatga gaagatcatc gtacttacta gcccaagaga tggttttcgg aatactttcc
      361 ccaggaacac ccagcttgag aagggtatcg tacggggact ctcctccgct gaaggtcttt
      421 accaaaccgc caaactccct ctcgatgagg gtgcatgtcc ggggaagacc tgcaaaataa
      481 acagcttcga taaagccacc ttgatagctg aagaggcttc ttctttgcat ctcgtttact
      541 agataaccaa ccatatttac cgcatcggct tcatccagcc tgtagctgat acctacccta
      601 ctgatccggt tttcaaccag ccagtttatc actacttctc ttctggattc gtagctgtag
      661 tccaaaatgg cctgctccac cgggccttct gcgatcagta cctcataccc gcagcttctt
      721 aggagttctg ctgcagagtt gatgcccagc gtgtgggcat ctaaactggg tttgatgaat
      781 ccatacctcc cgtgggatct aagcgccatg tgcatcactt ctttcggcag gactgagata
      841 tgttaacacg tctgagtttt cgctcaggta gtctaacgtg cgaccttctc gcctgaaatc
      901 aacgcccaac agcctgcagg caaggtcgat gatcagcgtg gtgttggaca tagacaaccc
      961 taaggtgtgt cctatggact caagaggcac caacccgaaa ggtatatctt cgtaaatgta
     1021 cctgtgctgc agcgagctcg gagcttcaat gtgcttgtac gcatcgttat tctgaatgca
     1081 ttcgtagaga tttctgcctt ttacgccgta tgttcgcttc agccagtctt gtgtggactc
     1141 cactctaagt cccagctttt cggagacggt tattctttcc cgatctatct cctccaaaaa
     1201 ctgagctata gtcggcgtaa taccgtcgta gtagtacttg tatatattgt ccttgttttc
     1261 cgtccatccc gcattgagta gcaacggtgc gcagtgcaga atcatgccca cattgcctat
     1321 ggaagtctcc accaaagact ttgcgggaac gaagtatctg cgaatgcact cgggaagcat
     1381 ttgaatgata gactcgttga gcgaggcatc cagagccgaa agcaggaccg aatctttcaa
     1441 tgcaatcacg tgtatggaat cttcgccagt ttttctgcag gtataaatcg tggtttgtgt
     1501 ttctgcaacc aagggttcgg atctggtatt gtatgcgctg aagacctttt tgaactctat
     1561 tgctccaaag gtccttccgg gattcaagac gatcagcaca gattctttca agttctctgc
     1621 aaccagtttt gcgagatccc tgtgggagtt ggcaggtgtg gtgactagta cgagatcggt
     1681 accttctaat gccaacgcta tgtcgctcgt gactaaattg attttcccat tcccacaaat
     1741 cacgccttcg cagtaaatgg tccctgtttc catgattttc gctatattct cttttgaccg
     1801 attccaaagc cttactttgt ttccgtataa gctcaaatga gcagccattg ccagtccaga
     1861 gtttcctgct ccaagaacag tcacgttcat atgcatcact ccttctgcat aagctgaaat
     1921 actgattact ctctatcctc ctgtggctcg aaaggtttac aatcagtccg aggcaagtgc
     1981 attttcggaa agcctaccag catgggtagc cgaaaacgtc tcttgagtct acaggggtta
     2041 ggggtcgcat cagaagcaga agagggattt agaaactaga ggcactagtc gcatgaactt
     2101 cctcatggtt agagaggtac ttaaaaagca ctcgcgtctt gatctacagt tgtgggggca
     2161 cggcagtcgg cactttccca gtcactttta tagccgaacc tgccttctaa caggccttgt
     2221 ccttacgaat caggtcgcag gagatctgtt taagaaatgg cctagctacc tttaagcatt
     2281 atatattata ccaagagtag gtctatgaac aaaattgaac tctaacacaa aagcttctag
     2341 ttggcacctg ataacacacc acttgacctc aaggatgtgt gcaactagaa gcttgttttg
     2401 tagatctgtt aagttcaagc tgacgacgca aagatctatc cgtgcatagt tattgctgat
     2461 ggttccgccc ccataacttc ctctttgtaa aatcaaccac aatacctttc tcacatggaa
     2521 gtgtacttag gggctctgcc tgtttgaagc tgacaagttt tcaagtcgcc agcctcataa
     2581 tttcgaacaa aaatgcggat ctacagaatc ttaactaagt ttgtctataa cgctccagac
     2641 tacagtgctg gcgttgtaag cagctttctg gaaaaccgaa tggagacttt ttgcagcctc
     2701 gctggctttt tgcatgggat ctgcatttac tgcagtgagt tctttttgca gagctttggt
     2761 gtcttgatag actagaggct cttttaggtc aattgcatcg tctgcatggt cgcttactcc
     2821 gcggacaact aggaagggta cgttcaaagc atgtttttg
//
LOCUS       DAOSDA010000238         2857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22630_length_2857_cov_3.224483, whole genome shotgun sequence.
ACCESSION   DAOSDA010000238 DAOSDA010000000
VERSION     DAOSDA010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22630_length_2857_cov_3.224483"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="PLW82_06025"
     CDS             <1..1149
                     /locus_tag="PLW82_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase-related protein"
                     /protein_id="HOJ46866.1"
                     /translation="GGGKVGPILLISNGHGEDILASELAKQLALEHEVVALPLVGEGT
                     AYDRAGIKKIHSQPLLPSGGFAYLGVKNLSRDVSAGLFAQLKEQKAALKGLRGVKLAV
                     CVGDTYNLFFTSRYLKCKKVFVPTAKSNYIKAHSFLDLLIMRKNALVVFPRDEITAKD
                     LRKRKVRAHYLGNLMMDALHPQGIHFGIDRFIGILPGTRDDAVINLKAILEVVSLLET
                     CNSFLVAMNDDGGGKSLLNDGWVEDQDPPHFAYRVLKKGEKKVYLCRDLFADIIAKAE
                     LVIGLSGTGNEQAAGLGKPVVTFVGPGAQFTPYFARKQKKLLGDAVLFVGEGKSPKEI
                     AEAVDRLYSDPELCRRMGQEGRKRMGAPGAARKMAELILAILKGEVVF"
     gene            1146..1865
                     /gene="lptB"
                     /locus_tag="PLW82_06030"
     CDS             1146..1865
                     /gene="lptB"
                     /locus_tag="PLW82_06030"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOJ46867.1"
                     /translation="MTIRAENLVKIYGQRKVVDEVTIEVNRGEVVGLLGPNGAGKTTT
                     FYMIVGIESVNGGRILLDGHDVTHLSMHKRALKGIGYLPQEPSVFRKLTVEENIMAIL
                     EARGADAKTQRHRTEELIEEFKLGVVRKTKGIRLSGGERRRTEIARALATDPSFILLD
                     EPFAGVDPIAVADIQEIIAMLREKNLGVLITDHNVRETLTITDRAYIMHEGHILVEGS
                     SKEIANDLDARRLYLGERFSL"
     gene            1862..>2857
                     /locus_tag="PLW82_06035"
     CDS             1862..>2857
                     /locus_tag="PLW82_06035"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOJ46868.1"
                     /translation="MKLLSILDRYICRELILPFLFGIVAFASIFVAADLVYLARVAVD
                     VGAPFLRAVELALMKFPQILVYVLPMSALMGVILTVGRMSQNSELIAIESSGQSFWRT
                     VRPIIGFGLAVTLFGLFVNDVMAPAAVTRYETLFQQMVRNKPVPVIKNNVILEEYQGG
                     LLKTLIYAAKFDPTTNTFTNVSYFTFVDGKPESTTEAEKMVWEDEAWYLENGRTFYYD
                     DLRTTVMTFARNTQPLSIVYKPQDVVLVNKDPSQMGIRELRQRIKLTSGQGNIRDLLI
                     EYHQKISIPFASLIFVLLGATLAASSPRSGSAIGFGLSIAIVFVYYVLLTLSSVLG"
BASE COUNT          768 a          633 c          741 g          715 t
ORIGIN      
        1 ggcggcggca aggtcggccc cattttgctg atcagcaacg ggcatggaga agacattctt
       61 gcttcagagc tcgcaaaaca actagcgctg gaacatgaag tggtagcgct gcctcttgtg
      121 ggagaaggaa cggcatacga ccgtgctggg ataaagaaga ttcattctca accactgctt
      181 cctagtggag gttttgcata cttgggcgta aagaatcttt caagagacgt ctctgccgga
      241 ttgtttgcgc agctcaaaga gcaaaaagca gctctgaagg gtttaagagg tgtcaaactt
      301 gcagtatgcg ttggtgacac ctacaatctg tttttcacat cgcggtattt aaaatgcaaa
      361 aaggtttttg tgcctacagc aaagtccaac tacatcaaag ctcattcttt cttagacctt
      421 ttaatcatga ggaagaatgc tttggtggtg tttccaaggg acgagattac cgcaaaggac
      481 cttagaaagc gcaaagtcag agcgcactat ttgggaaatc tgatgatgga tgccctgcat
      541 cctcaaggca tacactttgg tatagacagg tttattggca ttttgcccgg tacaagagat
      601 gacgcagtaa tcaacttaaa agccatactc gaggtggtct cgctgcttga gacctgcaac
      661 agcttcctag ttgctatgaa tgatgacggt ggtgggaagt ctctgctgaa tgacggctgg
      721 gtagaggatc aggatcctcc gcatttcgct tacagagtcc tgaaaaaggg agaaaaaaag
      781 gtctacctct gccgagatct ctttgccgat atcatcgcca aggcagagct ggttatcggt
      841 ctcagcggta ctggcaatga gcaggcagca ggcctgggca agccggtcgt gactttcgta
      901 ggtccggggg cccagtttac cccctatttt gcgagaaagc aaaagaaact tctcggcgat
      961 gcagtattat ttgtaggaga aggcaaaagc ccgaaggaga ttgccgaagc cgttgacagg
     1021 ctctacagcg atccggaact ctgcagaaga atgggccagg aaggccgcaa gagaatggga
     1081 gcacccggag ctgcacgcaa aatggcagag ctgatactgg ccatcctcaa aggggaggtg
     1141 gttttttgac catcagagca gaaaacctgg taaagatcta cggacagaga aaagtagttg
     1201 acgaagtaac tatagaagtc aatcgcggcg aagttgtggg acttctcgga cctaacggag
     1261 ccggaaaaac cacgaccttt tacatgatcg tcggtatcga aagcgtcaac ggaggccgga
     1321 ttctcttaga cggtcacgac gttacccacc tatccatgca caaacgagcc cttaagggaa
     1381 ttggctactt gcctcaggag ccttcggttt tccgtaagct cactgtggaa gagaacatca
     1441 tggccatcct ggaagctaga ggggcagatg ctaaaactca gagacatcgc accgaagaac
     1501 tgattgaaga gttcaaactg ggcgtggtaa ggaaaaccaa aggcattcgc ctttccgggg
     1561 gagaaagaag aaggacagag atagcaagag ctctagctac agacccatct tttattcttc
     1621 tcgacgaacc ttttgccgga gtagatccta ttgccgttgc tgacatacag gagatcattg
     1681 ccatgctccg ggaaaagaac ctcggcgttc taatcaccga tcacaacgtc agagaaactt
     1741 tgacgatcac agatagagcc tatattatgc acgaaggaca cattttagtt gaaggaagct
     1801 ccaaagaaat tgccaacgat ctagacgcaa gaaggcttta cctaggggag cgcttttctt
     1861 tatgaagctt ctatctattt tagacaggta tatttgcaga gagttgatcc tgccgttttt
     1921 gttcgggatc gtggcttttg ccagcatttt tgtagctgca gacctggtgt atcttgcccg
     1981 agttgcagtg gatgttggag ctccgtttct ccgagcagta gagcttgcac tgatgaaatt
     2041 tccccagatt ctggtttacg tgctccctat gtccgctctg atgggcgtga tattgactgt
     2101 cgggaggatg tcgcaaaaca gcgagctcat tgccatagag tccagcggtc agagcttttg
     2161 gcgtacggtt cgccctatta tcggttttgg ccttgcggtg acgctttttg ggctatttgt
     2221 aaacgacgtt atggctccag cagcagtcac caggtatgag actctctttc aacaaatggt
     2281 aagaaataaa ccggttcccg ttatcaaaaa taacgttatt ttagaggaat atcagggtgg
     2341 tcttttaaaa accttgatat atgctgcgaa gttcgacccg accaccaaca cctttaccaa
     2401 tgtaagttac ttcacgtttg tcgatggaaa accggaatca accaccgaag cggagaagat
     2461 ggtctgggaa gacgaggctt ggtacttgga gaacggacgc accttctatt atgacgatct
     2521 gcgcacaaca gtgatgactt tcgccagaaa cacgcagcct ttgtccatcg tctacaagcc
     2581 acaggatgtg gttttggtaa acaaggatcc cagtcaaatg gggattaggg aactaaggca
     2641 gcgtattaaa ctgacaagtg gtcagggaaa cattagagat ctgctcatcg agtatcacca
     2701 gaagatttct ataccgtttg ccagtttaat attcgttctg ttaggagcga cccttgctgc
     2761 ttcatcgcct aggtctggct ctgccattgg atttggactc agtattgcaa ttgtatttgt
     2821 ttactacgtc ctgcttactc tatcctcagt gttaggt
//
LOCUS       DAOSDA010000239         2856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22644_length_2856_cov_3.247412, whole genome shotgun sequence.
ACCESSION   DAOSDA010000239 DAOSDA010000000
VERSION     DAOSDA010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22644_length_2856_cov_3.247412"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /gene="rplI"
                     /locus_tag="PLW82_06040"
     CDS             complement(<1..246)
                     /gene="rplI"
                     /locus_tag="PLW82_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOJ46869.1"
                     /translation="MEVVLTQDVKSLGKKGDIVKVNEGYARNFLIPKGLAVVATEGVK
                     TQIKQQKKAEEKREEQKRAEAQALANKLKGQSVTVKAK"
     gene            complement(258..1241)
                     /locus_tag="PLW82_06045"
     CDS             complement(258..1241)
                     /locus_tag="PLW82_06045"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOJ46870.1"
                     /translation="MRLLIEEKSSLKWLEYIVLFSLMVLIVALSFMFFPASVYLALLI
                     PLPVLIATYRHSVLLGAVIAALSSVFFVFLLMNAGVVILIVALSTVGIGIGAAMREKL
                     DYKKFLAVGIISALLTVMLVGIGLYLTMGQTPKEIFNETADRILTELSTRDIPVQTEQ
                     VEALIETVRILYPSMIAVGAVALALVNFLAGSWALVRLKMTDVPALPPLTRWAFPMWL
                     SVAFIVVWVLTWQIFAFLPQPILFVVANLANFLHWLMTIQGLCIVLSFLRTIRLPIWG
                     IVLLVLPVWLFAPWFMALLGTLDPWLHFRGRIRLSQEERMRQLEKNKRDQN"
     gene            complement(1213..1545)
                     /locus_tag="PLW82_06050"
     CDS             complement(1213..1545)
                     /locus_tag="PLW82_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HOJ46871.1"
                     /translation="MNREIDIARNVKTIEWLRTEIVSYTAGLFKAMWQNREELILDSL
                     AGLIMAAYLLGKRLGIKFSRLESKVETKLKASIVEGHEIEKWYGDMTNLLDYWHDRRG
                     EIIDRGEE"
     gene            complement(1730..1969)
                     /gene="rpsR"
                     /locus_tag="PLW82_06055"
     CDS             complement(1730..1969)
                     /gene="rpsR"
                     /locus_tag="PLW82_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOJ46872.1"
                     /translation="MDNKRDKRKRRRKKVCSFCVDKMQTVDYKDVGRLRRYLSERGKI
                     LPRRINGNCAHHQRQMTLAIKRARFMALLPYVTED"
     gene            complement(1987..2403)
                     /gene="ssb"
                     /locus_tag="PLW82_06060"
     CDS             complement(1987..2403)
                     /gene="ssb"
                     /locus_tag="PLW82_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOJ46873.1"
                     /translation="MLNRVILIGRLTRDPELRYTTTGNAQARFTLAVDRDFTNQQGER
                     EADFIPVVAWGKLGEIVAHNLVKGRLVAVEGRISVRSYEAKDGTRRWVTDVNADTVKF
                     LEPKGGYSGSKDDSSFGDYDDSLESSGDEGLDEVPF"
     gene            complement(2422..2715)
                     /gene="rpsF"
                     /locus_tag="PLW82_06065"
     CDS             complement(2422..2715)
                     /gene="rpsF"
                     /locus_tag="PLW82_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOJ46874.1"
                     /translation="MRNYEMMYILNPTVADQEETLVSAIEKVNSLVTGNGGEIVNEDR
                     WGKRRFQYEINKIREGYYVVLKFKLNPESLDELNRVLKLNEDIVRYLLINDEN"
BASE COUNT          737 a          723 c          579 g          817 t
ORIGIN      
        1 cttagccttt accgttaccg actgcccttt aagtttattg gcaagagcct gagcttcggc
       61 tcttttttgt tcttctcttt tctcttcagc ttttttctgc tgtttgatct gtgtcttgac
      121 gccttctgtg gcaactaccg caagaccttt gggaatcaga aagtttcgag cgtatccttc
      181 attaaccttt actatatctc cttttttccc aagacttttc acgtcttgag tcaaaacaac
      241 ttccataagt aatcctccta gttctgatct cgtttatttt tctccagctg tctcatgcgc
      301 tcttcttggc tgagcctgat cctgccccta aagtgcagcc agggatcgag ggtacccaga
      361 agggccataa accacggagc aaaaagccat accggaagaa ctaaaagcac aatgccccag
      421 atcggcaggc gtatggtcct gagaaacgag aggactatgc agagtccctg aatggtcatg
      481 agccagtgta agaagttggc aaggttggcc acaacaaaca agataggctg tggcaggaaa
      541 gcgaagatct gccatgtgag aacccagacc acgataaatg ccacagagag ccacatggga
      601 aaggcccatc ttgtaagggg aggcagcgct ggaacatctg tcattttcag acgcacaagt
      661 gcccagcttc ctgcaaggaa gttgaccagc gcaagggcaa ctgcaccaac tgcgatcatc
      721 gacgggtaga gtatcctcac cgtctcgatc agcgcttcca cttgctcagt ctgtacgggg
      781 atatccctcg tgctaagttc tgtaaggatc ctatctgcag tttcgttaaa aatctctttt
      841 ggagtctgcc ccatagttaa gtacagacct atacctacca gcatcacagt taaaagcgca
      901 gagataatcc ccaccgcaag gaactttttg taatccaact tctccctcat ggcagcgcca
      961 atgccgatac ccactgtaga tagggcaact atcaaaatca ccacgcctgc attcatgagt
     1021 aagaaaacga aaaagacgct ggataaagct gctatcactg ctccaagaag cacgctgtgc
     1081 ctgtaagttg caataagcac aggcagggga atgagcagtg ctagatatac tgaagccgga
     1141 aagaacataa acgaaagcgc aacgataagt accattaatg aaaacaggac tatatattca
     1201 agccacttta gactactctt ctcctcgatc aataatctca ccccgtctgt cgtgccaata
     1261 atccagcaag tttgtcatgt ctccgtacca cttctctatt tcgtgtcctt caactatgct
     1321 tgctttgagt ttggtttcca ctttgctttc aagcctggaa aactttattc ccaacctttt
     1381 gccaagtagg tatgctgcca tgatgagacc tgctagtgaa tcgagtatca gctcttccct
     1441 gttctgccac atcgctttga agaggcctgc tgtgtaggat acgatctccg tacgtaacca
     1501 ctctatggtc ttcacgtttc tagcaatatc aatctctctg ttcatttcca cacctaaacc
     1561 tccttggaaa actaacgttc cctgttattt ccctagtact gttggttttc ctgctgtagt
     1621 gtgaaagatt gaaccgttag atctaggcct gcaacgattg ctcattatta atcatatcag
     1681 tttatagaag aaatgtataa caaaaaagga gggtttcccc tcctttggtt tagtcttcgg
     1741 taacgtatgg cagaagtgcc atgaaacgtg ctcttttgat ggccagagtc atctgtctct
     1801 ggtggtgagc acagttgcca ttgattcttc tgggcaagat cttgcctctt tcagaaagat
     1861 agcggcgcag ccttcccaca tctttgtaat caacggtctg cattttatct acacagaaag
     1921 aacaaacctt cttgcgacgg cgctttctct tgtctctctt gttgtccacg gtgtcaaacc
     1981 ctccttttaa aagggaactt cgtctaatcc ttcgtcgccg gaagattcta agctatcgtc
     2041 gtaatcacca aaggaactgt catctttaga accggagtat cctccttttg gttccaagaa
     2101 cttaacagta tcggcattga cgtcggtaac ccaacgacga gtgccatctt tggcttcata
     2161 acttctaacg cttatccgac cttctacggc tactaaccgc cctttgacta agttatgagc
     2221 gactatttct ccgagtttac cccacgcaac aactggaata aaatctgctt cccgttcgcc
     2281 ttgttgattg gtaaaatctc tatctaccgc aagggtaaac ctagcttgag cattaccagt
     2341 cgtggtgtat ctcaattctg ggtcacgggt tagtctgcca ataagaataa ctcgattcag
     2401 catccctaga ccccctaatg attagttttc gtcattgatc aacagatatc tgacaatgtc
     2461 ctcgttgagc ttcaatacac ggttgagctc atctaaagac tctgggttga gcttgaactt
     2521 gagtactaca tagtagcctt ctctgatctt gttgatttcg tactgaaatc ttctcttgcc
     2581 ccagcggtct tcgtttacaa tctctccgcc attgccggta accagcgagt tgactttttc
     2641 aatggcactt accaaagtct cctcctgatc agcgacagtg ggattcaaga tgtacatcat
     2701 ttcataattt ctcactgtca ttcacctccc ttcggtctat ggctctggac aaaccagagc
     2761 agggacatcg ggcatattat accactttca ggtagagttt aacaagtagt gaaaaaaacg
     2821 cggagttctt ttactattgg taaaactgga tacctg
//
LOCUS       DAOSDA010000240         2855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22651_length_2855_cov_3.247500, whole genome shotgun sequence.
ACCESSION   DAOSDA010000240 DAOSDA010000000
VERSION     DAOSDA010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22651_length_2855_cov_3.247500"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLW82_06070"
     CDS             complement(<1..559)
                     /locus_tag="PLW82_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ46875.1"
                     /translation="MKKAFNRWLLPGSYDLITGLREAKVAGFEGVELNVTVSGGEVSL
                     DTPKSELENLASEIEATGLEVTSVLPVEIFPNIAIDASAETIDLTLERLEKLLDICET
                     FNTDAILMVPGGLWSNEARYDQAYQNAKKVLTEMASLAEERDVILAVENVWNKFLVSP
                     LEMARFVDEIGSPCVKSYFDVGNVQA"
     gene            complement(730..2604)
                     /locus_tag="PLW82_06075"
     CDS             complement(730..2604)
                     /locus_tag="PLW82_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005550928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46876.1"
                     /translation="MSETRKMQGSPASGRKPQGPPIGPGGPGPGRRPVEKAKDFKGSL
                     KRLLTYLKPFKLQIVFILILAVLSSVFGIFAPKLMGEATTEIFGGIMTAAGVNFSRIA
                     RILVTLGTLYVFSAAFSYLQQYLMADISQKTVRDLRREISEKLNKLPLKYFDGRTHGE
                     VLSRVINDVDTISSTLQQSITQALSSLITLVGTLVMMLTISPTMTLLALISLPLALMV
                     TKFIIPRSQKYFVLQQRILGELNGHIEEAFGAHKIVKAYSNEQKAIDEFEEINEKLYD
                     TGWKAQFISGIIRPIMGFINNVGYVIICIVGSVMVTKGRIKIGDIQAFLQYSRQFTHP
                     IVGAADIINILQSTVASAERVFEILDEEEEVPDSPEAVEPKDVEGNVRFEHVTFSYEV
                     DKPLIEDLNIDVKSGQTVAIVGPTGAGKTTLVNLLMRFYELRGGRITIDGVDIKEMSR
                     SGLRRVFGMVLQDTWLFNGTILDNIAYGREGATEEEIVQAAVAARADHFIRTLPEGYD
                     TVLNEEASNLSQGQKQLLTIARAILADPAILILDEATSSVDTRTEVQIQKAMKTLMEG
                     RTNFVIAHRLSTILDADVILVMNHGQIIEKGTHKELLEKNGFYAELYNSQFTASQAAV
                     "
     gene            complement(2601..>2855)
                     /locus_tag="PLW82_06080"
     CDS             complement(2601..>2855)
                     /locus_tag="PLW82_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ46877.1"
                     /translation="IFDDSFSALDFKTDAQLRAALRRETQDATKLIVAQRVSTVLDAD
                     KTIVLDQGRVVGIGRHKELLTTSPVYREIVASQLSEEELA"
BASE COUNT          627 a          770 c          683 g          775 t
ORIGIN      
        1 aagcctgtac gtttcctaca tcgaagtagc tctttacgca tgggctgccg atctcgtcga
       61 cgaatcttgc catctccagc ggactgacca ggaacttgtt ccaaacgttt tccactgcca
      121 gaataacgtc tctttcctca gctagagatg ccatctctgt aaggaccttc ttggcgtttt
      181 ggtacgcctg atcgtaccta gcttcattgc tccaaaggcc tccgggcacc atcaaaatcg
      241 cgtcggtgtt gaacgtttcg cagatatcca ggagtttctc cagcctctcg agagtaaggt
      301 cgatggtctc ggcactggca tctatcgcta tgttggggaa gatctccaca ggcagcacac
      361 tggtgacctc aaggccggtg gcttcgatct cagatgccaa gttctcaagc tcgctcttag
      421 gggtgtcgag ggagacctcc ccacctgaca ctgtgacgtt tagttctacc ccttcaaacc
      481 ccgcaacttt agcttctctc agtccggtaa tcaggtcgta gctacccgga agcaaccacc
      541 tgttaaatgc cttcttcaat acaatcactc ccaacgccta ttttttccac ctgccgaaat
      601 aattccatca ttccctcagc tttcctgcta tatagggaaa tttcgtggtt ttcccgggta
      661 aacctccgtt tatggttaaa tttctaacca agatgccaat gggtgcaggt ctccctgcac
      721 ccactaacct tacaccgcag cttgcgatgc tgtgaactga ctgttgtata gttctgcgta
      781 gaagccgttt ttctcgagaa gctctttgtg ggttcccttc tcgataatct gaccgtgatt
      841 catcaccaag atcacgtctg catccaaaat cgtggacagc ctgtgtgcaa tgacgaagtt
      901 ggttctgcct tccatcaaag tcttcatagc cttttgaatc tggacctctg ttctggtgtc
      961 gacggagctg gtagcctcgt cgaggatcag gatcgctggg tccgccagaa tggctctagc
     1021 tatggtgagc agctgcttct gaccttggga gaggtttgat gcttcctcgt ttagaaccgt
     1081 gtcgtagcct tcaggcagcg tcctgatgaa atggtcggct ctagctgcaa ctgccgcttg
     1141 cacaatctct tcttcagtag cgccttctct accgtaggcg atgttgtcta gaatggtgcc
     1201 gttaaacagc caggtgtcct gcagcaccat gccaaagacg cgtctaaggc cgcttcttga
     1261 catctcttta atgtccactc catcgatagt aattcttcca ccgcggagct catagaaccg
     1321 catgagcagg ttaactagag tagtctttcc tgcgcctgtt ggtcccacga tggccactgt
     1381 ctgaccggat ttcacatcga tgtttagatc ttcaatcaaa ggtttgtcta cttcataact
     1441 gaaggtgacg tgctcgaatc taacattgcc ttccacgtct tttggctcca ccgcttctgg
     1501 gctgtcggga acctcttctt cttcatccag gatttcaaag accctttctg ctgaagctac
     1561 tgtggactgg agaatattga taatatccgc cgcaccgacg atagggtggg tgaactgtct
     1621 tgagtactgc aggaaagctt gaatatcacc gattttgatt ctgccctttg taaccatgac
     1681 gcttcctaca atacagatga tcacgtagcc gacgttgttg ataaagccca taatcggacg
     1741 aattatgccc gaaatgaact gggccttcca gccggtatcg tacagtttct cattgatctc
     1801 ttcgaactcg tcaatagcct tctgttcgtt gctgtaagct ttgacaatct tgtgcgcccc
     1861 gaaggcctct tcaatgtggc cgttaagctc gccgagaatg cgctgctgca acacgaagta
     1921 cttctgggat ctggggatga tgaacttcgt caccatcagg gctagcggca aactgatcag
     1981 agcgagaagc gtcatagtcg ggctgatggt cagcatcatc accaatgtac cgactagagt
     2041 aatcaaagat gaaagtgctt gggtgatgct ctgttggagg gtagaactga tagtgtcgac
     2101 gtcgttgatc actctagaga ggacttcacc gtgggttcgc ccgtcgaagt acttcagcgg
     2161 tagcttgtta agtttctcgc tgatctcccg gcgtaggtcc cttacggtct tctgggaaat
     2221 atcggccatc agatactgct gcagatagct gaatgccgcg ctaaacacat atagcgttcc
     2281 cagcgtcacg agtattcgag caatcctgct gaagttcacc cctgcagcgg tcatgatgcc
     2341 gccaaagatc tcggttgtcg cctctcccat gagcttagga gcgaaaatgc cgaaaaccga
     2401 cgagaggact gcgaggatca gtatgaaaac gatttgaagt ttgaaaggct tgagataggt
     2461 caaaaggcgc ttcagcgagc ccttgaagtc tttggccttt tccaccggtc tgcgcccagg
     2521 accaggtccg cccggaccta tcggaggacc ttgcggcttt ctgccagagg ccggacttcc
     2581 ttgcattttc cgtgtttcac tcatgctaac tcctcctcag acagctggga tgctacgatt
     2641 tcccgataga ccggcgatgt cgttagcagt tctttgtgtc taccaatacc cacaactctg
     2701 ccctggtcca gaacgatagt cttgtccgca tccaaaacgg tgctgaccct ctgagccacg
     2761 atcagcttcg ttgcgtcttg ggtctctctg cgaagtgcgg ctctcagctg tgcatctgtt
     2821 ttgaaatcca gagcggaaaa gctgtcatca aagat
//
LOCUS       DAOSDA010000241         2850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22724_length_2850_cov_2.758140, whole genome shotgun sequence.
ACCESSION   DAOSDA010000241 DAOSDA010000000
VERSION     DAOSDA010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22724_length_2850_cov_2.758140"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLW82_06085"
     CDS             complement(<1..399)
                     /locus_tag="PLW82_06085"
                     /inference="COORDINATES: protein motif:HMM:NF015383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HOJ46878.1"
                     /translation="MENQRNWRTDLAIDIQETLEGTEGFQTIPGVSKTVEETPVGDII
                     RLEVKDLQTAQIMHKAPGTYVTIEAPCLGLREKEKQNQVAMLLAKEIEKYLAPLPQDA
                     VILVIGLGNRNVTPDSLGPLVAEKVLVTRHL"
     gene            546..1289
                     /locus_tag="PLW82_06090"
     CDS             546..1289
                     /locus_tag="PLW82_06090"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46879.1"
                     /translation="MIDIHTHVLSNLRSDDGPKDPEKSRLMIQKAAEEGVTKIVATPH
                     MLTPIPERREEIYEAIRALGPTEAEIIPGSEIFADDVVLKSSESLICLGDSNYLLIEF
                     PRAQLPEYTEDIFFDLQTRGYECIFAHPERNGSIVNDPNILVDLIEAGVLVQINAGSI
                     LGRYGKDSQKTAEILLEHDMVHFIATDMHSDRNRYYSLKEAVEVAKKYTDRAVDLVTT
                     NPELILSGEYLEPPEPKEYKKRGFWFFRF"
     gene            1457..1717
                     /gene="rpsT"
                     /locus_tag="PLW82_06095"
     CDS             1457..1717
                     /gene="rpsT"
                     /locus_tag="PLW82_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOJ46880.1"
                     /translation="MANSKTAQKRILVSQRNSTQNRMVLSTMRTTIKKFLKAVEEGNK
                     ELAQESLVKAFSVIDKAAKKGVIHKNQADRRKSRLHKKLAAM"
     gene            complement(1816..2766)
                     /gene="holA"
                     /locus_tag="PLW82_06100"
     CDS             complement(1816..2766)
                     /gene="holA"
                     /locus_tag="PLW82_06100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOJ46881.1"
                     /translation="MAKPEKQIFLLLGEDEYLLEENAEKLTKGFTEVVKVSALDTELT
                     QIRNDLCSLGFFTDSKAVVIEDLEKLDVSTGEALVGLLEVVAENVRAVFKARKLEGNR
                     KWVKALKAGAELVDCSSPKPYEITKLLDKKIGELGLGRTEAEYLKEALPKDLLAAYNE
                     LEKLSLYPGKLSLSTLKLLIEPALENQVFAMMDFLVRQDLKNSLTTLKELLAAGNEPL
                     AVLGAIIWQIRTLYKVSGYSGSSPTKDLGISEFQYKKAAGALRQVSRGQIEQWFLLAL
                     DADMSIISGLFRPDAALEVLVAKMCLNLPPKKPLVVKSTS"
BASE COUNT          725 a          719 c          661 g          745 t
ORIGIN      
        1 gaggtgtctt gtaaccagta ctttttcagc aacaagcggt cctaacgagt ccggtgtaac
       61 gttgcggttg ccaaggccaa tgacaaggat cactgcatct tgaggtagcg gtgcaagata
      121 cttctcgatc tcctttgcca atagcattgc cacttggttc tgcttctctt tctccctaag
      181 tcccaaacac ggagcttcga tcgtgacgta agtccctgga gctttatgca tgatctgggc
      241 tgtctggagg tctttgacct ctaacctgat aatgtccccg acaggagtct cttcgaccgt
      301 cttactcacg ccggggattg tctgaaaacc ttcggttcct tctaaagtct cctggatgtc
      361 gatggccagg tccgtgcgcc agttgcgttg attttccatg tggttctcac ttccctctgc
      421 tttcgtcttt actagtttgt cccggtggat accggtcaat tcgacgtaca catgatgcag
      481 gacatctcaa agagaaactc gaaagctacc taaggaaatc ctttagttca aaggagtgaa
      541 cagtattgat cgatatccac actcacgttc tcagcaatct cagaagcgac gacggcccaa
      601 aagaccccga aaagagccgt ttaatgatac agaaagctgc agaagaggga gtcacgaaga
      661 tcgttgcaac tccccatatg cttacgccaa ttcccgagcg tagggaggag atctatgaag
      721 ccattcgagc tttgggacct accgaagcag agatcatccc tggaagcgag atctttgccg
      781 atgatgttgt tctgaaaagc tcggagagtc tcatctgctt aggtgatagc aactacctgc
      841 tgattgagtt tccccgcgct cagcttcccg agtacaccga ggacatcttt ttcgatctcc
      901 aaacccgagg ctacgagtgc atctttgccc atccagagcg gaacggttcg attgtgaacg
      961 accccaacat actcgtcgat ttaatcgaag ctggggttct cgtgcagatc aatgcaggca
     1021 gcattctcgg ccgctacggc aaagattccc agaagaccgc agagatcctt ttggagcacg
     1081 atatggtcca tttcatcgca acggacatgc attctgatag aaaccgctac tactccctta
     1141 aagaagcagt ggaagtggca aagaagtaca ctgatagggc tgtggatctt gtgaccacca
     1201 accccgagct catcttaagt ggggagtatc tagaaccgcc agaaccgaaa gagtacaaga
     1261 agaggggttt ttggtttttc aggttttagc tcatactaaa acggtaggtg tttaaaacac
     1321 ttgcattcga acccaaaagg ccttaaaatc taataagcag gaacatagct ggacagagcc
     1381 tggcagtgca gcttgtgttt aattgtatta aatggtatac ttaggcgtaa ttgtctttca
     1441 gggaggtggg atttagatgg caaacagtaa gacagcacag aagcgcattc tggtttctca
     1501 gcgcaacagc actcagaacc gcatggtact ttccactatg cgcactacaa tcaagaaatt
     1561 cctcaaagca gtggaagaag gcaataaaga gctggctcag gagtctttag ttaaagcctt
     1621 cagcgtgatc gacaaagctg ctaagaaagg cgttatccat aaaaatcagg cagatagacg
     1681 caagtcaagg ctccataaaa aacttgctgc aatgtaattg accgatcagc gatcggtctt
     1741 tttttcgcaa aaaacaccaa ccacttagag gttggtgttt tttgttatat gatatgcccc
     1801 tgtgtcccct ctgatttagc tggtggattt tacaaccaaa ggctttttgg gcgggaggtt
     1861 aaggcacatt ttggccacca agacctccag cgcagcatct gggcggaata ggccgcttat
     1921 aatgctcatg tcggcatcta gcgcgaggag aaaccactgt tcgatctgac ctctcgaaac
     1981 ctgtcttaaa gctcctgcag cctttttgta ctgaaactcg ctaatcccca agtcctttgt
     2041 gggactagat ccgctgtagc ccgacacctt atacagcgtc cggatttgcc agatgatcgc
     2101 gcctaaaact gcaagcggct cattcccagc agccaaaagt tctttcagtg tggtgaggga
     2161 gtttttcagg tcctgacgca ccaaaaaatc catcatggca aacacctggt tttccagggc
     2221 gggctctatc aagagcttca aggtgcttag gctcaatttc cccggataaa ggctgagttt
     2281 ttccagttca ttgtaagcag ccaagagatc cttggggaga gcctctttca ggtactcggc
     2341 ctctgtccgg ccgagcccaa gctcgccaat ctttttgtcc agcagcttgg tgatctcata
     2401 gggctttggc gatgaacagt caactagttc ggccccagct ttcaaggcct tcacccactt
     2461 gcggttgcct tctaacttcc ttgctttaaa gaccgccctt acgttctctg ccacaacctc
     2521 tagcaagcca acaagtgcct cgccggttga aacgtccagc ttctccaagt cttcgatcac
     2581 cacagccttg gagtctgtaa aaaagccgag gctgcagaga tcgtttctga tctgcgtcag
     2641 ttctgtatct agggcagaga ccttaacgac ctctgtaaag ccttttgtaa gcttttctgc
     2701 gttttcttca aggagatatt catcttctcc caaaagaagg aaaatctgct tttcaggttt
     2761 tgccatagcc ttatcccacc taaacgtacc ttattgtccc tgtaaatctt aagttttggt
     2821 gcaaaagcgc cactttgcca ctggctgact
//
LOCUS       DAOSDA010000242         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_22735_length_2849_cov_3.001432, whole genome shotgun sequence.
ACCESSION   DAOSDA010000242 DAOSDA010000000
VERSION     DAOSDA010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22735_length_2849_cov_3.001432"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="PLW82_06105"
     CDS             complement(<1..1422)
                     /locus_tag="PLW82_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46882.1"
                     /translation="MKKTLIVLIASFLLTLTVGANYYTLEGPHFFVHFPEGYEDLASK
                     LMVQAEEIMAELGEKMNLTINDKGHFVIEDSIDLSNGYATNTFYPLIAFYPVFPGDPL
                     LGGLGPDIDDWFETLLWHEGTHLYHVEMTKFPNQYITKIFGRYPTFYPIWSSPLSNIE
                     GFAVYNETHYQRVGRGSGSIYPMILDAAIDAGDLPDPKSTLGTYDLDRYDLAGLPYIY
                     GWNFLEFLADTYGENAPLMLQDYYVNTPGKAFMAQFDVALQKLTGKTFGTLSSEWYKW
                     MKENYDAPPLNPEGRIYDNLGDTIFPGFFDGEFFYYAMRGGSSGIYRIELKTGREELV
                     KYVPYIYGSISKDPSGGILYSSLDIDAQGNQYFNIYRYANRKTEAVVIGERAFSPVAT
                     KTGLYYLSQKHPEGNALYKRDLDGNITLLYLSKPGEEILQFAVSSKDEIYVSLWQEGG
                     FVDLGLLEDGKITFLTSDHYADSF"
     gene            1654..2712
                     /gene="glmU"
                     /locus_tag="PLW82_06110"
     CDS             1654..2712
                     /gene="glmU"
                     /locus_tag="PLW82_06110"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOJ46883.1"
                     /translation="MSDKVAVVILGAGKGTRMKSRMPKVLHKIGTRSMIEEVISTAKE
                     LNPEKIVVVVGFGADQVRQVLPGDVVSVEQTSQLGTGHAMLTALPELEDFDGHIITLY
                     GDVPLLSAGTIQELIKKHIKTEAAVTILTAILEDPTGYGRVIRNGDGTFFRIVEQKDA
                     SAAERRVKEINSGVYCFSSEVLKTYLPQLTPQNKQGEYYLTDVLGLASKDGLKVEMVV
                     AKDPVEITGVNDRVSLAMLTKIKNQRKLNKLMLSGVTIVDPDNTYIDDSVEIAMDASV
                     LPGTICIGECKIESGAVVGPYTTLVDCSVQDGAVVERSELKGVVVKSKAEIGPFVHVK
                     PGMDLAALGLADKIEFKD"
BASE COUNT          768 a          601 c          743 g          737 t
ORIGIN      
        1 gaacgaatcg gcatagtggt ctgaagtaag gaaagtgatc tttccgtctt caagaaggcc
       61 gaggtccaca aaaccgcctt cctgccagag agaaacgtag atctcatctt tactggacac
      121 tgcaaactgc aggatctctt cgccgggttt ggacaggtac agcagagtga tgttcccgtc
      181 caagtccctt ttgtaaaggg cgtttccttc tggatgcttt tggcttaggt agtagaggcc
      241 ggttttggtg gccacagggg agaaagccct ttctccaata actacagctt ctgtcttgcg
      301 gtttgcgtat ctatagatgt tgaaatactg gttgccctgg gcgtctatgt cgagggagga
      361 atacagaatt cctccggaag gatctttaga gatgctccca tagatatagg ggacgtattt
      421 gaccagctct tctctgcctg ttttcagttc gatgcggtag atgccgctcg atccacctcg
      481 catggcatag tagaagaatt ctccgtcgaa gaacccgggg aagatggtat ctcctaggtt
      541 gtcgtagatt ctgccttcag ggttgagagg aggagcatcg tagttttctt tcatccactt
      601 gtaccactca cttgaaagag ttccaaaggt ctttcccgtg agcttttgca aagccacgtc
      661 gaattgtgcc ataaacgctt ttcccggtgt gtttacgtag tagtcctgga gcatcagggg
      721 tgcgttttct ccgtaagtgt ctgcaagaaa ctccaagaag ttccaaccgt aaatataggg
      781 aagacctgcc agatcgtagc gatccaggtc gtaagttcct aaggtggact tgggatcggg
      841 gaggtctccg gcatctatgg ctgcatccag gatcatggga tagatgctgc ccgaaccgcg
      901 accgactctc tggtaatggg tttcgttata aactgcaaag ccttcgatgt tggacagtgg
      961 agacgaccag atggggtaga aggtaggata cctaccgaag attttggtga tatactggtt
     1021 tggaaacttg gtcatctcga catggtagag atgggtacct tcgtgccaaa ggagcgtctc
     1081 aaaccaatca tcaatgtccg ggcctagccc gcccaatagg ggatctcccg ggaaaacagg
     1141 gtaaaaagcg atcagcgggt agaacgtgtt ggtggcatag ccgtttgata gatcaataga
     1201 atcctcaatg acaaagtgac ctttgtcgtt gatggtcaga ttcatctttt cgcctagctc
     1261 tgccataatc tcctcagctt ggaccatcaa cttgcttgca aggtcttcat aaccttcggg
     1321 aaaatgtaca aagaagtgag gtccttctaa ggtgtagtag ttagcaccaa cagtgagagt
     1381 tagtaagaag cttgctatca gcacaattaa cgtctttttc atagactcta cccactttca
     1441 aaggatattg catttggaga tgttatgtac ttaacaaaca gttctctgaa atcggagtgt
     1501 ttccttcatt acccaggcga ttgcagacag cccctttgta atctgttggc aaacgttggt
     1561 tttgatgcag gaaaagacta atgaaaaatg aagataatag gtagaaatta acgacttcac
     1621 taaggattat tgcagataaa tggagggtta cggatgtcgg ataaagtggc ggtagtgatt
     1681 ctcggtgcag gtaaaggtac caggatgaaa agtagaatgc ccaaggtctt acataaaatt
     1741 ggcaccagga gtatgattga agaggtaatc agcacggcaa aagaactgaa tcccgaaaag
     1801 atcgttgttg tagtggggtt tggtgccgac caggtaagac aagtactgcc tggagatgtg
     1861 gtttctgtag agcagacttc tcaactggga acgggacacg ccatgctcac tgctctgcca
     1921 gagttagaag acttcgacgg acatatcatc acgctgtacg gcgatgtgcc actgctgtct
     1981 gcaggcacta ttcaagagtt gataaagaaa catatcaaga ccgaagcagc tgttaccata
     2041 ctcaccgcca tcctagagga tcctaccgga tacggcagag ttatacgcaa cggcgacggc
     2101 acgttcttta ggattgttga acagaaagat gcgtcagcag cagaacgccg agttaaagag
     2161 atcaactccg gagtttactg tttcagctct gaagttctca aaacctatct tccccaactg
     2221 actcctcaga acaaacaagg cgagtactac ctaacggacg tattaggtct ggcaagcaaa
     2281 gacggcttga aggtggagat ggttgtagct aaagatcctg tagagattac cggagttaac
     2341 gatagagtca gtcttgcaat gttaacgaag attaaaaatc aaagaaagct caataagctg
     2401 atgctttcgg gggttaccat cgtcgatccc gacaacacgt acatcgacga tagcgtggag
     2461 atcgctatgg acgccagtgt ccttccggga actatttgca ttggagagtg caaaatagaa
     2521 agcggtgcgg ttgtaggccc atacaccacg cttgtggact gttctgttca ggatggggct
     2581 gtggtagaaa ggtcagagct aaaaggtgtt gtggtgaaat ccaaagcgga gattgggccg
     2641 tttgtccatg ttaagcccgg tatggatctt gcagcgcttg gacttgcaga caagatcgaa
     2701 ttcaaggatt gaccccttct cagatgccct gcaaaagcca aaaggattta gtgccttctt
     2761 gacgaagcct tatcggcaat ttgctttttg atgcaagcat gctaaatgct ttttaatatc
     2821 agactggagg taaatttatg tcgttggtg
//
LOCUS       DAOSDA010000243         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_23205_length_2806_cov_4.066521, whole genome shotgun sequence.
ACCESSION   DAOSDA010000243 DAOSDA010000000
VERSION     DAOSDA010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23205_length_2806_cov_4.066521"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2325)
                     /gene="mutS"
                     /locus_tag="PLW82_06115"
     CDS             complement(<1..2325)
                     /gene="mutS"
                     /locus_tag="PLW82_06115"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOJ46884.1"
                     /translation="MSLDKLELIDISEMTPMLRQYYQIKMKYPDCILMFRLGDFYEMF
                     GEDAEIASKVLEITLTGRDAGSLGRLPMCGVPHHAVEGYLATLVENGYKVAICDQLED
                     PKLAKGLVKRDVTRVVTPGTITIPQALEDKANNYIAAIFGQGDAFGIAFADVTTGDFR
                     GTEIRGDKAEIKARDELLRLGPKEVIFPENFFAQSIVDTLDSLRIHYERRDENAFVAR
                     NSYQTLTRHFGVSTLMGYGIENQPALISAAGALLSFLEDTQKIALEHIKSIQAYALTD
                     YMVLDVSTQRNLEILSTLRENKKQGSLLGVLDKTVTAMGGRGLRRWLSQPLIDLAAVK
                     SRQDRVEAFFNDDLLRARVRGLLDKVFDLERLAGRLAYRTANARDLVSLKESFKRLPE
                     LKELLSGITVLSEVEELDALEDLTEIIEEAVVDDPPFTLTEGGIIKRNYNQELDSLRE
                     ISAGGKEWIANLQQKERERSGIKSLKVGFNKVFGYYIEVTKANLDMVPDDYERKQTLA
                     NGERYITPELKEYEAKVLGAEEKIKTLEYELFVEVRERLTQNIERIQKVAKIITELDI
                     YTTLAEVARQKNYTKPEVYLGDELEIVEGRHPVVEEYLPQGEFVPNDTYLDNDDKRIA
                     ILTGPNMSGKSTYMRQVALICLMAQIGSFVPAKKCRIGLVDRIFTRVGASDDLASGQS
                     TFMVEMTEVANILHNATEKSLVILDEVGRGTATFDGLSIAWAVTEYLHDRIGCKAIFA
                     THYHELTQIEETLPCVFNLQVAVQHSGKQVFFLRK"
     gene            complement(2347..2691)
                     /locus_tag="PLW82_06120"
     CDS             complement(2347..2691)
                     /locus_tag="PLW82_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46885.1"
                     /translation="MDDVRIGDLLFTARCDIEGMDVFSLDKKLLGAIIFQVVDVPAFT
                     VRYLGVVSGEQTFLVPAELILDVDDLGLIMDFEQKIFAKLLPLPESWEEVFARDYEEK
                     IYSTLNLTPYWQ"
BASE COUNT          650 a          768 c          641 g          747 t
ORIGIN      
        1 ctttctgaga aaaaacacct gcttgcccga gtgctgcact gcaacctgca ggttgaacac
       61 gcagggcagg gtctcctcga tctgagtgag ctcgtgatag tgagtggcaa atatggcttt
      121 gcagccgatc cgatcgtgca ggtactcggt aacggcccac gcgatactga ggccgtcaaa
      181 ggtagcggtg ccccggccga cctcatccag gataaccaag ctcttttcag tggcgttgtg
      241 cagaatgttt gccacttccg tcatctccac cataaacgtc gactgacctg acgccagatc
      301 gtccgaagct ccaactctgg tgaagatgcg gtctacaagg ccgattctgc actttttcgc
      361 cggcacaaaa gagccgatct gcgccatcag gcagatcagg gctacctgtc gcatatacgt
      421 cgatttaccg gacatattag gtccagtaag tatggcaatc cgcttgtcat cgttgtccaa
      481 ataggtgtcg tttggtacaa actctccctg cggtaggtat tcctcaacca cagggtggcg
      541 accttcaacg atctccagct catcaccgag atagacttcc ggtttggtat agttcttctg
      601 ccttgccact tctgcaaggg tagtatagat atctaactcc gtgatgattt tcgctacttt
      661 ttggattctc tcgatgtttt gggtaagcct ttcccttacc tccacaaaca gctcgtactc
      721 cagggttttg atcttttcct cagcacctaa taccttggct tcgtactcct ttagctcagg
      781 ggtgatgtac ctttcgccgt ttgccagtgt ctgtttgcgt tcgtagtcgt cggggaccat
      841 atccaaattg gctttggtca cctcaatgta gtacccaaac accttgttaa agcctacttt
      901 cagcgacttg atgccgcttc tttcccgctc cttctgttgg aggttggcga tccactcttt
      961 acctccagcg gagatctccc gcaaactgtc cagctcctgg ttgtagtttc tcttgataat
     1021 gccgccttct gtgagggtaa acggcggatc gtctacaacc gcttcttcga tgatctctgt
     1081 aagatcctcc agggcatcca gctcctcaac ttcggaaaga acggttatcc ccgaaagaag
     1141 ctctttaagt tcggggagtc ttttgaagga ctccttgaga gacaccagat cccgagcatt
     1201 agcggtcctg tacgccaagc gacctgcaag cctttcaagg tcgaaaacct tatcgagaag
     1261 gccgcgtact ctggccctga gaaggtcgtc gttaaaaaaa gcttcaaccc gatcctgtct
     1321 gctttttaca gcagcaaggt cgattaaagg ctgggaaagc cacctccgca gacctctgcc
     1381 tcccatagcg gtgactgtct tgtccaaaac gcccagaagg gaaccctgct ttttgttctc
     1441 cctcaaagtg ctaagtattt ccagattgcg ctgggtagaa acgtccagca ccatgtagtc
     1501 agtaagtgcg taagcttgaa tgctctttat gtgctccagg gcaatcttct gcgtgtcttc
     1561 caaaaagctt agaagagccc cagctgcaga aatcaatgca ggttgatttt cgatgccgta
     1621 acccatcaag gtggaaacac cgaagtgtct ggtcaaagtc tgatagctgt ttcttgccac
     1681 aaaagcgttt tcgtctctgc gttcgtagtg aatccttaag ctgtccagcg tgtccactat
     1741 gctttgagca aagaagttct ctgggaaaat cacctctttg ggacctagcc tgaggagctc
     1801 atctctagct ttaatctcgg ccttatctcc ccggatctcg gttccccgga aatccccagt
     1861 ggtgacatcg gcaaaagcga tgccaaacgc atcgccctgt ccgaaaatcg ccgctatata
     1921 attgttggct ttgtcttcca aagcctgggg gatggtgata gtgccagggg tcactaccct
     1981 agttacatcc ctcttcacca ggcctttagc tagcttagga tcttcaagct ggtcgcagat
     2041 ggcaacttta tatccgtttt ccaccagtgt tgccagatac ccctcgaccg catggtgcgg
     2101 cacgccgcac atgggtaggc ggccaagaga tcctgcatct ctaccggtga gagttatctc
     2161 caaaaccttg gacgcgatct ccgcatcctc accaaacatt tcgtagaaat cgcctaggcg
     2221 aaacatcagg atgcaatctg gatatttcat tttaatctgg tagtactgcc tcagcatcgg
     2281 cgtcatttcg ctgatgtcga tcaattcaag cttgtccaga ctcatcgtat cacctcttac
     2341 ccaaacctac tgccaataag gagttaggtt cagggtgctg tagatcttct cttcgtaatc
     2401 tctggcaaag acttcctccc agctctcagg cagcgggagc agtttggcaa agatcttctg
     2461 ctcgaaatcc atgatcaagc ccaggtcgtc tacatccaga atcagttcag caggaacgag
     2521 gaaagtctgc tctccactga caactccaag gtacctaacg gtgaaagcgg gaacgtctac
     2581 aacctgaaag atgatggctc ccaaaagctt tttatcgagg gagaaaacat ccataccctc
     2641 aatgtcgcac cgggctgtaa acagaagatc gccgattctg acgtcgtcca ttctctttta
     2701 ctcctttagc tttccttcaa gtgaggacag tttacctgca gttacttcaa cctggacagt
     2761 cctgccgata agacctacat caccagggaa attgatagtt ttattt
//
LOCUS       DAOSDA010000244         2801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_23262_length_2801_cov_3.477058, whole genome shotgun sequence.
ACCESSION   DAOSDA010000244 DAOSDA010000000
VERSION     DAOSDA010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23262_length_2801_cov_3.477058"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PLW82_06125"
     CDS             <1..875
                     /locus_tag="PLW82_06125"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOJ46886.1"
                     /translation="QAMGADVHVEGGYVIAKASRLKGARIVTDMVTVTGTENLMMAAV
                     LAEGRTIIENVAREPEVVELQRFLNTLGANVHGAGTDTIVVEGVEFGQLKGADYTVIP
                     DRIEAGTFLIAGAITGGNVKVYGARADHNEALLYKLHEAGVNLNIGPNFIEVAKGPKA
                     RFTPLRIRTMPYPGFPTDLHPQMTALLALADGTSLITETVFPDRFKYVHELIRLGANI
                     SVEGECAIVKGVPKLKGAKVEATDLRGGAALVVAALAASGDSTVQKLEHLDRGYENLT
                     EKLLSLGALVERYN"
     gene            950..1600
                     /locus_tag="PLW82_06130"
     CDS             950..1600
                     /locus_tag="PLW82_06130"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOJ46887.1"
                     /translation="MKQKRRCLIFLLLALSGFLLVTQVFRVKEVTVVGAVLANEGSIR
                     RVIGQPNLLWLDLKELEDRIAQDSFIKKASIEVVGLSSLRVTIEERKPAANFDLSGTL
                     IQVSRDGTILSVGEDNVLPTIYGLAVFPFMPGQAVPSEYIYLLELVEATRDLGLEEIV
                     SDDGKLTLKISDGCEVFLGTNLESPDVIRQKLKEIALRGQKHRYIDLSQKGYARGL"
     gene            1617..2678
                     /gene="ftsZ"
                     /locus_tag="PLW82_06135"
     CDS             1617..2678
                     /gene="ftsZ"
                     /locus_tag="PLW82_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOJ46888.1"
                     /translation="MLEFDMDRPQIGSIKVIGIGGGGSNAVNRMIEAQVKGVEFIAMN
                     TDAQALTLSKADTKLQLGEKLTRGLGAGSNPEVGKKAAEESREEILSLVSGSDMVFIA
                     AGMGGGTGTGAAPVVAEIAREAGALTVGVVTKPFFFEGKKRMIQAEAGLACLKDKVDT
                     LIVIPNDKLLNIAEKNTTLIDAFKIADDVLRQGVQGISDLIAVPGIINLDFADVKAIM
                     SSAGTAIMGIGTAKGDDRALNAAEQAISSPLLETSIMGAKGILLSITGSSDIRLLEIT
                     EAAKIISESADPDANIIFGAVIDEKLEDEVRITVIATGFDVKGEKGADEAAATKEEIK
                     PFSTGHDIDIPIFLRKPRS"
BASE COUNT          735 a          654 c          770 g          642 t
ORIGIN      
        1 ttcaggcgat gggcgccgac gttcacgtcg agggcggcta cgtcatcgcc aaggcgtccc
       61 gcctgaaggg cgcgcgcatc gtgacggaca tggtcacggt caccggaacc gaaaacctca
      121 tgatggccgc tgttcttgcc gaaggtagaa ccatcattga aaacgtagcc agagaaccgg
      181 aggttgtgga actacagcgg ttcttgaaca cccttggcgc aaacgtgcac ggagccggca
      241 ccgacactat tgtggtggag ggcgtggagt tcgggcaact gaaaggcgcc gactacacgg
      301 ttatccccga tcggattgaa gcgggaacct tcctcatagc cggcgcgatt accggtggca
      361 acgtgaaggt ctacggagca agagcggacc acaatgaagc tctactttac aaactccacg
      421 aggcaggagt aaacttaaac atcgggccca acttcatcga ggtggcaaaa ggaccgaaag
      481 caaggttcac gccgctgaga attcgaacca tgccgtaccc aggttttccc accgacttgc
      541 atccgcagat gaccgctctg cttgcactag cagatggcac cagtttaatc actgaaacag
      601 tttttcccga caggttcaag tacgttcacg agctcatcag actcggtgca aacatctctg
      661 ttgaaggaga gtgcgccatc gttaaaggcg ttccgaaact caaaggggcc aaagtagaag
      721 caacggatct tcgggggggt gctgcccttg tggtagcagc tctggcagcc tcaggcgact
      781 ctacagtgca gaagttggaa catctggaca ggggctacga aaacctcacg gagaagcttt
      841 tgtctctagg ggcactggtg gagcggtaca attagcaggg gctgaagcag gaaatgccaa
      901 acaataacag aaatatggtc ttaagagtag tttgaggagg ccatcttcta tgaagcagaa
      961 gagacgctgc ttgatctttc tcctcttggc attgtctggt tttttgctag tcacccaggt
     1021 tttcagggtt aaagaggtca ccgtagttgg cgctgtactg gccaatgaag gtagtatcag
     1081 aagggtcatt ggccagccaa accttctgtg gttggatctt aaagagctgg aagatcgtat
     1141 agcccaagac agcttcatta aaaaagcaag catcgaagtg gtagggcttt ccagtcttag
     1201 ggtcactatt gaggaaagga agcctgcagc aaacttcgat ctatcaggta ccctgattca
     1261 ggtgtcaaga gatggcacca ttctttccgt cggagaagac aacgtcctac caaccattta
     1321 cgggcttgcg gttttcccat ttatgccggg acaagccgtt cccagcgagt acatttacct
     1381 tttggaactg gttgaagcca caagggacct gggtttggag gagattgtca gcgatgatgg
     1441 caagctaact ctgaagattt ccgacggttg cgaagtgttt ttgggaacca atttggaatc
     1501 acccgatgtt atacgacaga agttgaaaga aatcgcatta cgcggacaga agcacagata
     1561 cattgatttg agccaaaaag gctacgcacg tggcctttaa ggtaggaggg gaacatatgc
     1621 tggagtttga tatggacaga cctcagatcg ggagcattaa agtaataggt attggtggcg
     1681 gcggcagcaa cgcggtaaac cgcatgatcg aggcacaagt taagggtgtg gagttcattg
     1741 ccatgaacac cgatgcccag gctttgactc tgtcaaaggc cgacactaag ctgcagctcg
     1801 gagaaaagct caccagaggt cttggcgctg gctccaatcc ggaagtcggt aaaaaggccg
     1861 ccgaagagtc ccgcgaagag atcctcagcc tggttagcgg ctccgatatg gtgtttattg
     1921 cagctggtat gggcggaggt actggaacag gtgctgctcc cgttgttgct gaaatcgccc
     1981 gggaagcagg agctcttacc gttggcgttg ttaccaagcc gttttttttc gaaggcaaaa
     2041 agaggatgat acaagcggaa gccggccttg cctgcctcaa ggataaggtc gataccctga
     2101 tcgttatacc taacgacaag ttgctgaaca tcgcagagaa gaacacaacc cttatcgatg
     2161 cgtttaaaat cgccgatgac gtccttcgcc agggcgtgca gggcatttcc gacttgatcg
     2221 ctgttccggg aatcatcaat ttggattttg ccgacgtcaa ggcgattatg tcgtctgcag
     2281 gaaccgctat tatgggtatc ggtaccgcca aaggcgacga cagagcgctc aacgctgcag
     2341 agcaggcgat ttcttcgccc cttttggaaa caagcattat gggcgcaaag ggcatccttc
     2401 tcagcataac tggttcttcc gacattcgcc tgttggagat cactgaagct gcaaagatca
     2461 tctccgagtc tgccgatccc gatgccaaca tcatctttgg tgccgtgatc gatgagaaac
     2521 tcgaggatga ggttcgcatt accgttatcg ccaccggctt tgatgttaaa ggagaaaaag
     2581 gtgcagatga agctgctgca acaaaagaag agattaaacc cttttccaca ggtcacgata
     2641 tcgacatacc gatcttttta aggaaaccca gaagctaaac atttcctttt tgcaggaaat
     2701 tctgccttct caagtaatta ttaggtaatg aagggaaggt ggcagtaata ttttggtatt
     2761 gctggtgcta atggatatta tgatgcaatt cttcagtata t
//
LOCUS       DAOSDA010000245         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_23378_length_2792_cov_2.962368, whole genome shotgun sequence.
ACCESSION   DAOSDA010000245 DAOSDA010000000
VERSION     DAOSDA010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23378_length_2792_cov_2.962368"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /gene="mreB"
                     /locus_tag="PLW82_06140"
     CDS             complement(<1..591)
                     /gene="mreB"
                     /locus_tag="PLW82_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HOJ46889.1"
                     /translation="MAFGIYRDLGIDLGTANTLVYMKGKGIVLREPSVVALDTNTDEV
                     LAVGHEANRMVGRTPSNIVAVRPLREGVIADLEVAEKMLRHYITSVLKKHWFTKPRVL
                     VAVPSGVTEVEKRAVVDAAKQAGAKEAKLILEPMAAAIGAGLPIQDAVGSMIVDIGGG
                     TTEVAVISLGGIVTQQSRRSAGDEMDMPIVQYVRRTH"
     gene            complement(607..1287)
                     /gene="radC"
                     /locus_tag="PLW82_06145"
     CDS             complement(607..1287)
                     /gene="radC"
                     /locus_tag="PLW82_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOJ46890.1"
                     /translation="MWRIKELPETERPREKLARWGAAALSNQELLAIILGSGYQGVSA
                     ISLADKLLCRSGGLMGLKDISLTELLEVPGIGEAKAVGILALVELSRRLHTFEPALDS
                     AIESPSAVFDLLAATVADLDREKCIVLLLDNRHRLLEVVTISIGTLDASLVHPRELFK
                     SCIRRSAAGVVLVHNHPSGDPRPSLEDKMLTARIVEAGRILGIRVLDHVILAGQSYYS
                     MREHGLMD"
     gene            complement(1298..1873)
                     /locus_tag="PLW82_06150"
     CDS             complement(1298..1873)
                     /locus_tag="PLW82_06150"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012095630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOJ46891.1"
                     /translation="MMGLILASKSPRRRELLDLLGIDYTVYPAEINEGIDEADPEILV
                     KKLASLKADHVAKIHPKDFVLAADTIVCLGDKVLGKPKDAEDARAMLTLLSGKTHNVY
                     TGVALKSLEHRFAKIISVATEVDFREISPDEIEFYIASQEPFDKAGGYGIQGFAGVFV
                     TAIRGSYHNVVGLPLAQTAQLLGEAKLYKLW"
     gene            2088..>2792
                     /locus_tag="PLW82_06155"
     CDS             2088..>2792
                     /locus_tag="PLW82_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ46892.1"
                     /translation="MKLIFLGTGAGKPSKMRNVSGFALDMVENYGYAFLFDCGEGTQH
                     QIMYTNLKLSHIKCIFITHLHGDHIYGLPGLLGTRSFENQKDPLTVYGPKGIKTFLDT
                     VLEVSGTHLTYPLEVVEVNSGDVFHFDRFTIEVALLEHVVPSFGYRLVETSPESLDAD
                     KLKELGIPPGPIYGRLKNRETVVVDGKVLSGEDFLLPPKVRRIVTICGDTRKCAVSVQ
                     LARDATVLVHEATHMVD"
BASE COUNT          677 a          780 c          644 g          691 t
ORIGIN      
        1 gtgggtgcgg cggacgtact ggactattgg catatccatt tcgtcaccgg cagaacgcct
       61 ggactgctgg gttacaatac ctcccagtga gattacggca acctcggtgg tgccgccacc
      121 gatgtctacg atcatgctgc ctactgcatc ctggatgggc agtcctgcac cgatagccgc
      181 ggccataggc tctaagatga gctttgcctc tttggctcct gcctgttttg ccgcatctac
      241 tacggcgcgt ttttccacct cagttacgcc gctgggaacg gcaaccagta ctctgggttt
      301 ggtaaaccag tgcttcttca aaaccgaggt gatgtagtgc ctgagcatct tttcagccac
      361 ttcgaggtcg gcaatgactc cctcgcggag cggtctaacc gctacgatat tcgaaggggt
      421 tctccctacc attctgttgg cttcgtgtcc caccgctagc acctcatcgg tgttggtgtc
      481 cagcgcaacc acagaaggct ccctcagaac aattccctta cctttcatgt acaccagcgt
      541 gtttgcggtg cccaaatcga ttcccaaatc tctatatata ccaaaggcca tctttcttgc
      601 ccctttctaa tccatcaaac catgctctct catactgtag tagctctggc ctgctagtat
      661 cacgtggtct aaaacgcgga tgccaagaat cctcccggct tcaactatcc ttgcggtaag
      721 cattttgtcc tccaaagacg gcctgggatc tccgctggga tggttgtgca ccagcacgac
      781 ccctgcagca gaacgcctaa tgcagctctt aaagagctcc ctcggatgga caagggatgc
      841 atccaatgtg ccgatggaga tggtcaccac ttccagcagt cggtgacggt tgtccaaaag
      901 cagcacaatg cacttttctc tgtcaagatc ggcgacagtg gccgccaaaa gatcaaagac
      961 ggcgctgggg ctctcaatgg cactgtccag cgcaggctca aaagtatgga gcctgcggga
     1021 aagttctacc aaagctagga taccgacggc tttagcctca ccgatccccg gcacctccag
     1081 aagctccgta agggaaatat ccttcaggcc cattaggcct ccgcttctgc agagaagctt
     1141 gtccgctaga gaaatagcag atacaccctg gtaaccgctg ccgaggatta tcgccagaag
     1201 ctcctgattg gaaagagctg ctgctcccca tcttgccagc ttctccctag gtctttcagt
     1261 ctcgggcaac tccttgattc gccacataac aatcctccta ccacagttta tacagtttgg
     1321 cttcgcccaa aagctgagcg gtctgggcta aaggtaagcc aactacgttg tggtaggaac
     1381 ctctaatggc tgttacaaag actccagcaa aaccctgtat gccgtaccca cctgccttgt
     1441 caaagggctc ttgacttgct atatagaact caatctcatc ggggctaatc tcgcgaaagt
     1501 ccacctctgt ggcaacagag atgatcttag cgaatcggtg ctccagactc ttgagcgcaa
     1561 ctccggtgta gacgttgtgg gtttttccgc tgagaagcgt cagcatagct ctagcgtctt
     1621 ctgcgtcttt gggctttccc aagaccttat ctcccagaca gacaatggtg tccgctgcca
     1681 gaacaaagtc tttaggatgt attttggcaa catgatctgc tttaagtgaa gccagtttct
     1741 taacaagaat ttccggatcc gcttcatcaa ttccttcatt tatttcagcg ggataaacgg
     1801 tataatcgat tcctagtaaa tctagaagtt cgcgccgcct gggcgactta gatgctagaa
     1861 ttagtcccat aattattcct cttttcagtt taccactggc tatcggctaa aacaagcatt
     1921 ctacaggcct cgtgggcttg tccatactaa aaaatcttat taatattgtt aactctcttt
     1981 tcgcgaaaat caaagaaaga cctctttaat aatctttcct tttaagttag aaatagaggt
     2041 agcgttaagt ggtaataaat agataataat ttctacaggt gatcaaaatg aaactaatct
     2101 tcctgggcac aggagcaggt aaacccagca aaatgcggaa cgtctcgggc tttgccctgg
     2161 acatggtgga aaactacggc tacgccttcc tttttgactg cggcgaaggc actcagcacc
     2221 aaatcatgta cacaaacctc aaactctccc atatcaaatg catctttatc acccacctcc
     2281 acggcgacca catctacggt ctgccggggc ttttgggcac aaggtccttt gaaaaccaaa
     2341 aggatcccct taccgtctac ggccctaagg ggattaaaac cttcctcgat acggtccttg
     2401 aagtcagcgg aacccacctc acctatcccc tggaagttgt agaggtaaac tcaggagacg
     2461 tcttccactt tgaccgcttc accatcgaag tcgctctcct agaacacgtg gttcccagct
     2521 tcggctacag gctggtggaa acctctcctg aatccctgga tgcagataag ctgaaggaac
     2581 tgggcattcc gccaggcccc atctacggca ggttgaaaaa ccgcgaaacg gtggtggtag
     2641 acgggaaggt gctctccggc gaagacttcc tccttccacc aaaagtccgg aggatcgtga
     2701 ccatctgcgg cgacaccagg aagtgtgctg tttctgttca gcttgcaaga gacgcaacgg
     2761 tgctagtcca cgaagcaacc cacatggtgg at
//
LOCUS       DAOSDA010000246         2788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_23407_length_2788_cov_3.870838, whole genome shotgun sequence.
ACCESSION   DAOSDA010000246 DAOSDA010000000
VERSION     DAOSDA010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23407_length_2788_cov_3.870838"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLW82_06160"
     CDS             <1..606
                     /locus_tag="PLW82_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOJ46893.1"
                     /translation="HQVGVKLSGLDDDFLLFATEMGDYLDLSKGLRCKVSSWRHVSDN
                     AIPMRCKCSGAYANAALIKTEALDDGYDEAIVLTDDGHVSEGSAENIFIVRGGKLVTP
                     PETDDLLPGITRDTVMVLAEKVLNIPVEIRRIDRTELYIAEEAFFCGTGAQIAPITSV
                     DNRPIGNGEVGPISKKMQEIYFQVVRGQLKEFSHWCTPVFK"
     gene            690..1523
                     /locus_tag="PLW82_06165"
     CDS             690..1523
                     /locus_tag="PLW82_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46894.1"
                     /translation="MSIVFCLVFLLCFSQVGSASFSAQMDLVKPSVSFTVRGDKESWF
                     RGFSVDADEKSQKWQTTFRIKETGDFGTNSLDFVTAAWQKDQELGADFSFVWQSGHAT
                     LQGKKTLSRNGSKWSPKIIVDFQPYFDAKVQYEQLTQGGKWQVTYRKKTGDLTFSSSF
                     SYYKEPTCSLSCTYDPGPADLSFSLGFGKGGVTKTAARIGFDQERVKGFLKVDVDRYT
                     KGTVALQLDIDKLKIDAKVQDAGIDVGLSCAISSGDFTFQPSLSFGSKGLGLGFRIKI
                     A"
     gene            1853..>2788
                     /gene="gcvT"
                     /locus_tag="PLW82_06170"
     CDS             1853..>2788
                     /gene="gcvT"
                     /locus_tag="PLW82_06170"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOJ46895.1"
                     /translation="MLRKTPLYSWHVSEGANMTEFAGYQMPLHYKTGIIEEHKNVRLR
                     AGLFDLCHMGQIQVKGRQAGEYLDYVLTQKVSDMPDGLVRYGLLLDFSGGTIDDLMVY
                     KRKEDYLLVVNAANKEIDYTWLLEQKGLFDVEINDLSDSYGIIALQGPASENILAPLL
                     ADGLGNLKYLRFIETELLEVPATVSRTGYTGTDGFEIYLPLQAIPALWNLLLEEGKSF
                     GLLPIGLGARDTLRLEMGYSLYGHELSREINPLEAGLKWAIKFDKDDFIGKDALLKVK
                     EEGVKRELLAFKTEGRVIPRNGYKIIADDKEVGFVT"
BASE COUNT          738 a          636 c          741 g          673 t
ORIGIN      
        1 caccaggttg gcgttaaact ttccgggctc gatgacgatt ttctgctttt tgctacggaa
       61 atgggcgact atttggatct cagcaaaggc ctcaggtgca aagtttcctc ctggcgccac
      121 gtatccgata acgccattcc catgcgctgc aagtgcagcg gagcgtacgc caatgctgcg
      181 ctgatcaaga ccgaggcact agacgacggc tacgacgaag ccatagtcct taccgacgat
      241 ggccacgtat ctgagggcag cgcggagaac atctttattg ttagaggtgg gaagctcgtc
      301 acgcctcctg aaaccgacga tctcctcccg ggcattacca gagacacggt tatggttctg
      361 gcagagaagg tcttgaacat ccctgtagag attcgccgca tcgacagaac agaactctac
      421 atcgccgaag aagctttctt ctgcggtaca ggtgcccaga tcgctccgat cacctcggtg
      481 gacaacagac cgatcggcaa tggcgaggta ggacctattt ccaagaaaat gcaggagatc
      541 tatttccaag ttgttcgcgg tcagctgaaa gagttcagcc actggtgtac accagtcttc
      601 aaataaggta aaataaagaa aagaaatcct ccttactgac tttcttccta gagcaacacc
      661 tagggaggag tagtacaagg aggtttttta tgtccatagt gttctgtttg gtgtttttgc
      721 tgtgcttttc tcaagtggga tctgcaagct tctcagctca aatggacctg gttaagccca
      781 gcgtctcgtt tacagttcgg ggcgataagg agagctggtt tcggggcttc agcgttgatg
      841 ctgatgagaa gagtcagaag tggcagacta ccttccggat taaagagact ggggactttg
      901 gtaccaattc tctggatttt gtcaccgcag cttggcagaa ggatcaggaa ttaggggcgg
      961 acttctcttt tgtctggcag agcgggcatg caactcttca gggaaaaaag accctaagcc
     1021 gaaacggctc taagtggagc ccaaagatca tcgtggactt tcagccctac ttcgacgcaa
     1081 aggtgcagta cgaacagctg acccaagggg gcaagtggca ggtcacctac agaaagaaaa
     1141 ctggtgatct caccttttcc tcatcttttt cctattacaa ggagcctacc tgcagccttt
     1201 cctgcaccta cgatcctgga cctgcggact tgagcttcag cttaggtttt ggtaaaggcg
     1261 gtgtaaccaa aaccgctgct agaatcggct ttgatcagga gcgggttaaa ggttttttga
     1321 aggtggatgt agatcgctat accaaaggta cagtggctct gcagcttgat attgataagc
     1381 ttaagattga tgcaaaagtt caggatgcag gcatagatgt gggactttcc tgcgccatta
     1441 gttctggaga tttcacattc caaccgtctt tgagcttcgg ttctaaagga ctgggtttgg
     1501 gctttagaat aaaaatagcc tgagaagact caggctatct cctagcgatg atgatgcaag
     1561 tgcgctttct ccacctgcac cacggagcca gaatgagttt gagaagtgca accacagcaa
     1621 aacctcctct taggtgagag ttgttgatgc aaacgtcttc cttcagcaca gcctaccaag
     1681 caattttcca cttgtttcca gaaatcggaa ggtagaaaac acaaccagta gaaactatct
     1741 tttaggagga aagatgtggg tcccgcgtgt tgcgtagact aaaaacagag ggttactgag
     1801 aaaacaagtg ctaaggatcg gaagcgtttg gcataaacaa ggagggggtt aagtgcttag
     1861 aaagacaccg ctttacagct ggcacgtaag cgaaggagcc aacatgacgg agtttgcagg
     1921 ctaccagatg cctcttcact acaaaaccgg tattatcgaa gagcacaaaa atgttcgctt
     1981 gcgggcggga ctatttgatc tttgtcacat ggggcaaatc caggttaagg ggcggcaggc
     2041 cggcgagtac ctggactatg tgttgaccca gaaggtatcg gatatgcccg atggtctggt
     2101 gcgttacggt ctcctcttgg acttttcagg aggtaccatc gatgatctta tggtatacaa
     2161 aagaaaagag gactacctct tagttgtcaa tgcagctaac aaggaaatag actacacttg
     2221 gctgctggag caaaaaggcc tctttgacgt agagatcaac gatctttctg actcatacgg
     2281 cattattgcc ctgcaggggc cggcttcgga aaacattctg gcaccgctcc ttgcagacgg
     2341 cctcggcaat ctcaaatacc tgaggtttat cgagacagag cttttggaag tgccggcgac
     2401 ggtttcccgg acgggataca ccggtacaga cggttttgaa atctaccttc ctctgcaggc
     2461 tattccagca ctgtggaatt tgctactgga agagggaaaa tccttcggtc ttttgcctat
     2521 agggctcgga gcaagagaca ccttgcgcct ggagatgggc tacagcctgt acggacacga
     2581 gctgtctcgg gagatcaacc cgctagaggc agggctgaag tgggcaatca agttcgacaa
     2641 agatgacttc atcggcaaag atgccctttt gaaggtcaaa gaagaaggcg taaagagaga
     2701 gcttttagcc ttcaagactg aaggcagggt tattccaaga aacggctaca aaatcattgc
     2761 agacgataaa gaagtgggtt ttgtaact
//
LOCUS       DAOSDA010000247         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_23851_length_2751_cov_4.066024, whole genome shotgun sequence.
ACCESSION   DAOSDA010000247 DAOSDA010000000
VERSION     DAOSDA010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23851_length_2751_cov_4.066024"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLW82_06175"
     CDS             <1..274
                     /locus_tag="PLW82_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46896.1"
                     /translation="LAAKESGASYVLRAPLTLPSKLDKVFYAFLEKNYRELIQPYRFL
                     YGKEGRVPESYWRPIDEMIETLAREQGLCTEAPYWTEVQSKQLSLF"
     gene            complement(310..510)
                     /locus_tag="PLW82_06180"
     CDS             complement(310..510)
                     /locus_tag="PLW82_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ46897.1"
                     /translation="MPTYVYKCPKCGNFDYYQNMNDSPLQNCPTCGSTVKKLISAGSG
                     IIYKTNGFYITDSKDKKDTAAS"
     gene            complement(629..1486)
                     /locus_tag="PLW82_06185"
     CDS             complement(629..1486)
                     /locus_tag="PLW82_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HOJ46898.1"
                     /translation="MERIFYVNGEFVPADQAVVSAEDRGFNFGDGVYEVVHWYNYRPF
                     LLEEHVERLIHSAQVIRIDLPWSKEELTEIALETAKRNNLKDDYIYMQISRGRAIRQH
                     SFPKNVQPTLVMNPQPAAKPPEEVRQKGARVISIPDIRWLHCDIKALSALANILAKQQ
                     AKDHNVDDVLYDRAGVGVVEGGSSNIFYYKDGTLYTPPLSPYIFPGITRAKVIEIAGE
                     LDIPLGEKVFSLKEVLEADEVMLVSTGYEVLSVTEVNGLKIGNGVPGEIAKKLQDRYT
                     EHVRKACYV"
     gene            1740..>2751
                     /locus_tag="PLW82_06190"
     CDS             1740..>2751
                     /locus_tag="PLW82_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46899.1"
                     /translation="MNRRYLTVIIVTLFFSLFVSAEDKLNLASILEGGSVVVSEPTKD
                     SVALQTILDGVSGTGGVVFTFGPSEKGSILIELGGNSLNKIDEVVFTAKVPIWTAGQA
                     LEIKVKTAEKAEGPFVDAGSLKLTYSGALGSVFEPRAAKYVLLEFGTTSSQTENVIIE
                     EVEIYGVPLDEPEPEPERVLTQSKINLFEHLGLEIKWELTGFAIVSPEPHEATEERIG
                     IDVKTSDIKARFNVDGRLASRANLTGNITTPKNWEKLFLPETIYVRCYYLEEERAWLV
                     GSQTLILNEMNNEVELTLSLGANRSWPNGDYRIEFYVGENPPLTYYFSIEEARVDVDT
                     AVD"
BASE COUNT          697 a          632 c          678 g          744 t
ORIGIN      
        1 tcttgcagct aaagagtcag gcgcaagcta cgttttaaga gcaccattga cgctcccaag
       61 caagctcgat aaggtgtttt acgctttctt ggagaagaat tacagagaat tgattcaacc
      121 atacaggttc ttgtacggta aagagggcag ggtacccgag tcttactggc ggcctatcga
      181 cgagatgatc gaaacccttg ccagagaaca agggctctgc accgaagcac cgtactggac
      241 ggaagtgcag tccaaacagc tgtcgctgtt ttaaggcata aaaagaggag cattttgtat
      301 gctcctcttt tatgatgctg ccgtatcttt tttgtctttg gagtcagtga tgtagaaacc
      361 gtttgtctta tagataatac cagatccagc agaaatcagc tttttgacgg tactgccgca
      421 tgttgggcag ttctgcaggg gactgtcgtt catgttttga tagtagtcga agtttccgca
      481 tttcggacac ttatagacat atgttggcat cttaggcacc tccatgcgtt tctagattca
      541 cttttgcgtt taaaagcaag gcggggttgg tcaacccctt taaactctaa tgattatgat
      601 attccctttg tcccaaaaaa gcaatagttt aaacgtagca agccttcctg acgtgttcgg
      661 tgtacctgtc ctgaagcttt ttggcaatct cgcctggaac gccattgcca atctttagac
      721 cattaacttc ggtaacgctg agcacttcgt atccggtgct gacaagcatg acttcgtcag
      781 cttccagaac ttcctttaag gagaacactt tctccccaag aggaatgtcg agctcgcctg
      841 cgatttcaat gactttcgct ctggtgatgc ccgggaagat gtatggcgat agtggagggg
      901 tgtagagagt gccgtcttta tagtagaaga tgttgctaga gccgccttct acaacgccta
      961 cgcccgcccg atcatagagc acgtcgtcga cgttgtggtc tttagcctgc tgttttgcga
     1021 ggatatttgc caaagcgctc aaggccttga tgtcgcagtg aagccatctg atgtccggaa
     1081 tgctgataac tctcgcacct ttctgtcgga cttcctcagg tggtttggct gcaggctgcg
     1141 ggttcatcac caatgtgggc tgaacgtttt ttgggaagga gtgttgccta atagctctgc
     1201 ctctcgagat ttgcatgtag atgtagtcgt cttttaggtt gttgcgcttg gctgtttcaa
     1261 gtgcaatctc tgtcagctct tctttggacc aaggcagatc gatcctgata acttgcgcgc
     1321 tgtggatgag tctttcaacg tgctcttcca acaaaaacgg ccgatagtta taccagtgaa
     1381 ctacttcgta tacaccgtcg ccgaagttaa aacctcgatc ttcggcagag acaactgctt
     1441 ggtctgcagg gacaaattca ccgttaacgt aaaaaattct ttccaatcca atctcctccc
     1501 actgcttttt cattcctatc tattgtaccc aaaaatccgc caaactgcat ccgacctttt
     1561 gagaggattt acggattttg cccttgagtt cgatataatc agcttatacc tttacacagg
     1621 ataattcaac aggtgcacgg ccaatcagag aaatacgcga gttttagaag gaaacgtcgg
     1681 ctggtgtaca ataaaatata aagacaacta ttatgttttc gtgaattgga ggaagtacga
     1741 tgaaccgccg ataccttaca gtaatcatag tcactttatt cttttctctc ttcgtatcag
     1801 ctgaagacaa actcaacctc gcctcgattc ttgagggagg ttctgtcgtg gtcagtgaac
     1861 ctacaaagga ctcagtggcc ttgcagacca tacttgatgg cgtaagcggc acaggtggag
     1921 tggttttcac ctttggcccg agcgagaaag ggagcatcct tattgagctt ggcggaaata
     1981 gcttgaacaa gatcgacgag gtcgtcttca ctgctaaggt gcccatttgg actgcaggtc
     2041 aagctctgga gatcaaagtc aagactgcag agaaagccga aggtcctttc gtcgatgccg
     2101 gcagtttgaa gcttacctat tctggtgctc tgggttctgt ttttgaaccg agagccgcca
     2161 aatacgtgct gctagagttt ggtaccactt caagccaaac ggaaaacgtg atcattgaag
     2221 aagtagagat atacggtgta cctctggatg agcccgagcc agagcctgag cgagtcctga
     2281 cccagtccaa gattaacctc tttgaacatc tgggtctgga gatcaagtgg gaactgacgg
     2341 gatttgccat tgtcagccca gagccccacg aagcaactga agagcgcatc ggtattgacg
     2401 tcaagacaag cgacataaaa gcccgtttca acgtcgatgg ccggcttgca agcagagcaa
     2461 acctcacagg aaacatcacc actccgaaaa actgggagaa gctcttcctg ccggagacga
     2521 tctatgtgcg ttgctactac ttagaggagg aaagggcttg gctggtaggt agtcagacgc
     2581 tgattttaaa cgagatgaac aacgaagtgg aactgacact ttctttggga gcaaatcgca
     2641 gctggcctaa tggcgattac cgcatagagt tttatgtcgg ggaaaatccg ccccttacct
     2701 attatttttc aattgaggag gccagagtag atgttgatac tgccgtggat g
//
LOCUS       DAOSDA010000248         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_23914_length_2745_cov_3.421561, whole genome shotgun sequence.
ACCESSION   DAOSDA010000248 DAOSDA010000000
VERSION     DAOSDA010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23914_length_2745_cov_3.421561"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLW82_06195"
     CDS             complement(<1..206)
                     /locus_tag="PLW82_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HOJ46900.1"
                     /translation="MRKFVSPTKGRLSQEDLYRDIVAFIEADRKSQYSLIVGTDSQPK
                     SDAAVFVTAIVVHRVGRGARYYYM"
     gene            complement(279..1118)
                     /gene="rsmA"
                     /locus_tag="PLW82_06200"
     CDS             complement(279..1118)
                     /gene="rsmA"
                     /locus_tag="PLW82_06200"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOJ46901.1"
                     /translation="MEKRSEQDRVASAKRVKELLQTYGMKAKKSFGQNFLIERSVVER
                     ITDALEISERDVVLEIGPGLGALTEALLERRAKVFAVELDRDMVLILEETLKREYTNL
                     EVINEDFLKSQWERFGAAKIAGNLPYYISTPILMEILRKNTPWEKLAFTLQWEVVQKI
                     TARPGEENYSVLSCAVQCFGEPEVLMKLPPHYFLPQPGVHSGVILIARKDLDLDEKTL
                     EVLWQLLDNAFRNRRKTLQNNLAHLLPEGFETSIDLSLRAEALPPGEFVTLAKEICEF
                     FGK"
     gene            complement(1143..2165)
                     /locus_tag="PLW82_06205"
     CDS             complement(1143..2165)
                     /locus_tag="PLW82_06205"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HOJ46902.1"
                     /translation="MGRVFKLASGVFLLITSVFFLVAGVSHQRYVPVTVHVGDNAITW
                     KTPMKTVKSTLSELGIEVNPQDEVYPSLDAPITQNMHVTIIRRFDISVKDGWVDGGKA
                     KTYTVSAQTVGDALKEIGIELSGADTIVPALDSPIGRGSTITITRRDSYYQTEYELIP
                     YGLWEFSDNRIYEGTSQVWRQGESGTRAKTYLVKRENGVIVSRELVEDVVTKAPKLHV
                     VAKGTKPRYYTLKTPAGNILYTKKITIEATAYYPGPESTGIYADGYTYTGVKAGHGII
                     AVDPKVIPLGTKVYIPGYGFAIAADIGGAIKGNIIDLCFDTYREAIQFGRRMVELYFV
                     VDEGRL"
     gene            complement(2178..>2745)
                     /locus_tag="PLW82_06210"
     CDS             complement(2178..>2745)
                     /locus_tag="PLW82_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HOJ46903.1"
                     /translation="KLDDTLYINLTNSCTNACTFCIRNGRDGLDGYDLWLERCPTAEE
                     IIAEIPDPSVFPEIVFCGFGEPTMRLEVLKAVAKYIKNQGGITRLNTNGHGSLINKRD
                     IVPELKGLIDTVSVSLNAGSKEEYDRVSVPQIDGAFEATVQFIKRSVEILPRTIATVV
                     RVPGIDIEKARSLAEELGAEFRVRELEE"
BASE COUNT          591 a          724 c          680 g          750 t
ORIGIN      
        1 ttcatgtagt agtagcgcgc tccccgtccc acgcggtgga cgacgattgc ggtaacaaac
       61 actgccgcat cgcttttggg ctgagagtcg gtaccaacta ttaagctgta ctgcgacttg
      121 cggtcggctt caataaaagc aacgatgtcg cggtagaggt cctcttgtga caaacgtcct
      181 tttgtggggc tgacaaattt ccgcatgttt tatcaccttg tttcctttgt cagagagttt
      241 cgacacggca aaaggtgaac cctgccccca aaacttggct acttgccaaa aaattcacag
      301 atttccttgg caagtgtaac aaattcccct ggcggtagcg cttctgctct caaggaaaga
      361 tcgatgctgg tctcaaagcc ttccgggagg aggtgcgcca agttgttctg cagcgttttt
      421 ctccgattcc tgaaggcatt gtccaaaagc tgccagagga cttccagggt tttttcgtcc
      481 aagtccaggt cttttctggc gatcaagatt acgccgctgt gcacacctgg ctggggcaga
      541 aagtaatgag ggggaagctt cattagcact tcaggctctc cgaagcactg cacggcacac
      601 gagagcacgc tgtagttctc ctctccggga cgtgcggtga tcttctgcac tacctcccat
      661 tgaagggtaa aggccaactt ctcccagggg gtgttctttc gcaaaatctc catgagaatg
      721 ggagtggaga tgtagtaggg aaggttgcct gcgattttag cagcaccaaa tctctcccat
      781 tggctcttta agaagtcttc gttaatcact tctagattag tatattctct tttcagcgtt
      841 tcttccaaga tcagtaccat gtccctgtcg agctcaacgg caaacacttt agccctgcgc
      901 tcaaggagcg cttcggtgag ggctccaagc ccaggcccga tctccaaaac cacatcgcgc
      961 tcggagatct ccaaggcatc tgttatcctc tccaccacag acctttcgat gaggaagttc
     1021 tgtccgaagc tttttttggc cttcatgcca taggtctgta ggagttcttt aacccttttt
     1081 gccgaagcaa ccctatcctg ctcactacgc ttttccattt ttgcctccta aaaaagggtc
     1141 gctcataggc gaccctcatc tacaacgaaa tatagctcca ccatacgcct gccaaactga
     1201 atggcctccc tataggtgtc gaagcacagg tcgatgatgt tgcccttgat ggcgccgccg
     1261 atatctgcag cgatggcaaa cccgtagccg gggatgtaga cttttgtgcc aagtggtatg
     1321 accttgggat ccacagcgat gatgccgtgt ccggctttga ccccggtgta ggtatagccg
     1381 tcagcgtaaa tgccggtgct ttccggtccg ggatagtaag cagtggcttc gatggtgatc
     1441 tttttggtgt aaagaatgtt tcctgctggc gtctttaagg tgtagtatct gggctttgtg
     1501 cctttagcta ccacgtggag tttcggagct tttgtcacta catcttccac cagttctctg
     1561 gagacaatta cgccgttttc ccgctttacc aggtaggttt tggccctagt tccgctttct
     1621 ccctgcctcc agacctgcga ggttccctcg tagatgcgat tatcggagaa ctcccacagt
     1681 ccgtagggta tcagttcgta ttcggtttga tagtagctat ctcttctggt gatggtgatc
     1741 gtggaccctc tacctatggg gctatcaaga gcgggaacaa tcgtatcagc accgctgagt
     1801 tcgatgccta tctcctttaa ggcatccccc accgtctggg cagaaacggt ataggtcttt
     1861 gcctttcctc catctaccca gccgtctttg acggagatat cgaacctgcg gatgatggta
     1921 acgtgcatgt tctgagtgat cggagcatcc aaggaaggat aaacctcatc ttgtgggttt
     1981 acttcaatgc ccagctcgct taaggtgctt tttactgttt tcattggagt cttccaggtg
     2041 atagcattat cacctacatg cactgtgacg ggtacgtacc tctggtgaga tactcctgca
     2101 actaagaaga atacgctggt tatcagcagg aatacaccgg aggccaactt aaatacccta
     2161 cccacaaaat ccctccttta ctcctcgagt tcgcggactc tgaactccgc gccaagttct
     2221 tcagcaagac ttcttgcctt ctctatatcg attccgggca ctctgactac cgtggcgatg
     2281 gtgcggggga ggatctccac cgacctttta atgaactgga cagttgcctc aaatgctcca
     2341 tcgatttgcg gtacagagac ccggtcgtac tcttctttgc taccggcatt gagggacacg
     2401 ctgacggtgt cgatgaggcc tttgagttcg ggaacaatgt cccgtttatt gatcagagag
     2461 ccgtgcccgt tggtgttcag cctggtgatg ccgccctgat tcttgatgta ttttgctacc
     2521 gccttcagca cttcaagcct catggtaggc tcgccgaatc cgcagaagac gatctccggg
     2581 aaaaccgagg gatccggtat ctcggcgatg atctcctctg ctgtagggca acgttcgagc
     2641 cagagatcgt atccatcaag gccgtctcta ccgttacgaa tgcagaaagt gcaggcattt
     2701 gtgcagctgt ttgtcagatt gatgtaaaga gtatcatcta gtttg
//
LOCUS       DAOSDA010000249         2740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_23955_length_2740_cov_3.271508, whole genome shotgun sequence.
ACCESSION   DAOSDA010000249 DAOSDA010000000
VERSION     DAOSDA010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23955_length_2740_cov_3.271508"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /gene="glgD"
                     /locus_tag="PLW82_06215"
     CDS             <1..1114
                     /gene="glgD"
                     /locus_tag="PLW82_06215"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOJ46904.1"
                     /translation="LMGAIILADNQVNLKELTLRRQLAAVPFGARYRLIDFPLSNLVN
                     AGVRNVGVFVQEKYRSLMDHIGIGKEWDLNRQRDGLFILPPEAYSDGAVPNAHSGDLA
                     LLRRHIDYFFRSKQEYVVVCCGSIIMNIDLTQMLTKHLNTGADITLLYRVEKDRTDHS
                     QAVEVFTDENSRVTFLKVNPIVSSSSKILLDTYILRRDLLIELVDAAVSSGGSDFVMD
                     VLIRNLGDLKVYGHEFSGYARKICSLETYFNASMELLTPEVYSELFFANGSIFTKVKN
                     EPPVVYLDGATAKNNLIANGCRIEGTVENSILFRGVKVEKGAHVKDSIIMQKSVVGRG
                     AFLENVIVDKDSQISSHKRLVGDRTYPLVIGKGSAL"
     gene            1164..2594
                     /gene="glgA"
                     /locus_tag="PLW82_06220"
                     /pseudo
     CDS             1164..2594
                     /gene="glgA"
                     /locus_tag="PLW82_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
BASE COUNT          722 a          664 c          712 g          642 t
ORIGIN      
        1 ccttatgggt gcaataatcc tggcggacaa tcaagttaac ctcaaagagc taacgttgcg
       61 caggcagctt gctgcagttc cattcggagc acggtatcgc ttaatcgact tccccttgtc
      121 gaacctggtc aatgcaggtg tgcgcaacgt aggggtgttc gttcaggaga agtaccgctc
      181 cctgatggac cacatcggaa ttggcaagga atgggatctg aacaggcaaa gagacggact
      241 gttcatcctg cctcctgaag cttacagcga tggtgcagtg cccaatgcgc actcaggaga
      301 ccttgcgctt ttgcggaggc acatcgatta ctttttccgc agcaagcagg agtatgtggt
      361 ggtctgttgt ggatcgatta taatgaacat agatctaaca cagatgctta caaaacactt
      421 aaacaccggt gcagatatca cgcttttgta ccgggttgag aaagaccgta ccgaccacag
      481 tcaagctgtg gaggtgttta ccgacgaaaa ctccagagta acctttctta aggtgaaccc
      541 gatcgtcagc agctcttcca agatcctcct cgatacctat atcctcagga gggatcttct
      601 cattgagctg gtggacgctg cggtctcttc aggaggcagc gactttgtta tggatgtgtt
      661 aatccggaat ctcggggacc tcaaagtcta cggacacgag ttttcggggt atgccagaaa
      721 aatctgttct ctggaaacct actttaatgc cagcatggaa ctgttaactc ccgaggttta
      781 ctcggagctg ttctttgcca acggctcaat ctttacaaag gtcaagaatg aaccccccgt
      841 tgtctatcta gatggtgcga ctgcaaaaaa caacttgatc gccaacgggt gcagaataga
      901 aggtaccgta gaaaacagca ttctttttcg cggggtgaaa gttgaaaaag gtgcccacgt
      961 caaggacagc atcatcatgc agaagtctgt ggtaggtagg ggagcgtttt tggagaatgt
     1021 tatcgtagat aaagactccc agatttcgtc acataaacgc cttgtaggcg acagaaccta
     1081 tccgctggtg atagggaagg ggtcagcatt gtagcgccgt ggccagattg gtcatggcct
     1141 tttctaaaag aggagggtgt aagatgaagg ttttgtttgc agccagcgag tgcgtaccgt
     1201 ttttcaagtc gggaggtctc ggagatgtgg tcggttcgct gcctaaggct ttgaaggacc
     1261 tgggtataga tgtgagggtg gtcctgccca agtaccacac tctggaagac cggttccgct
     1321 atatgctgag gcaaaaagcc gttttgaacg tgaaggtcgg ctggcgcaat cagtactgcg
     1381 gcgttgaaga actagaactg gacggagtac atttttactt catcgataac gagtactact
     1441 tcaagaggga gtctttgtac ggctacctcg atgatgcaga aagattcatc ttcttctcaa
     1501 gggcggtgct tcaagcacta ccctacatgg acttctgacc ggacatcatt catcttaacg
     1561 actggcagac ggcaatcatc gcaactcttc tcaacgccca ccacgaaaga gacttcttct
     1621 acactggcat gaagaccgtc ttgaccattc acaacctccg gtatcagggg acctttcaaa
     1681 aagaagttct tacggacatg ctcgacttgg gttgggagta cttcgacaac gataagctcg
     1741 aacacgacgg aggcatctcg ctcctgaagg gaggcatcgt ctcctccgat gctctcacca
     1801 ccgttagtcc cagctacgct caggagatcc aggatccttt ctacggtgag gggtttgatg
     1861 ggctcctgag ggctatgaga agcaaattaa cgggaataat aaacgggata gactataccg
     1921 agtggaatcc agcagccgat ccgcatatct ttacaaccta cgacggaaat tctttggata
     1981 aaaagctaga gaacaagctg cgtctgcagg aaagcttggg actgaaaaaa gatccaagca
     2041 taccggtaat tagcatggtt tcgcggctgg tctcacaaaa aggtctggat cttgtagctc
     2101 atgtctttga cgagatactc gaaaaaaacg tccagtttgc cgtactgggc accggcgaaa
     2161 gtaaatacga agaccgtttc ctcgaaaagg caagaaagcg tccctttcag gtatctgcca
     2221 acattcggtt cgacaacgtc ctggcccacc aggtctacgc aggttccgat tacgttctca
     2281 tgccttcgat gtttgaaccc tgcggactta cgcagctcat tgctctcaga tacggtaccg
     2341 tccctatcgt ccgcatcacc gggggactaa gggacacagt acaggcatac gacgaaacga
     2401 caggaaaggg gaacggattc aacttcctga catacaatgc ccacgacatg ctagatgccg
     2461 tgggcagagc tctaggtgtc tacaacaggg agccccactg gactcagctg cgccaaaacg
     2521 ccatcagcga tgatcacagc tggggcagtt ctgccaaagc gtataaggaa ctctacgaat
     2581 ccctcatgag ttaaatcttt cggaaaggtg tcttcagggt gaaaactaca gcactaagct
     2641 atgacagcga ggagttcatc aaaaattata cctacaacgg cagcctgggg gctctctggt
     2701 cgcctgagaa gaccgagttc aggctctggg ctccaacggc
//
LOCUS       DAOSDA010000250         2704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_24434_length_2704_cov_2.987542, whole genome shotgun sequence.
ACCESSION   DAOSDA010000250 DAOSDA010000000
VERSION     DAOSDA010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24434_length_2704_cov_2.987542"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PLW82_06225"
     CDS             complement(<1..291)
                     /locus_tag="PLW82_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46905.1"
                     /translation="MDTRAKASELTTTGLLLAVSLVLCFVGVLFPTFGTYLKYLIPGM
                     MAVIKTKYGYRYLTAFSLALLILVAVFFGIEQMVTGLFFVIPIGFALALVYED"
     gene            complement(474..830)
                     /locus_tag="PLW82_06230"
     CDS             complement(474..830)
                     /locus_tag="PLW82_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46906.1"
                     /translation="MRVLLIAPHRKAEALLKLLRRDLHLAKSAGSFDAAKRFLGQGED
                     LIVFTPEVAADVVKGFGVLKEATEARLLYVVDFPTDGFLWQLLSLGIDGYATLDAENF
                     RLALSELEMNRYGFVE"
     gene            complement(802..1842)
                     /locus_tag="PLW82_06235"
     CDS             complement(802..1842)
                     /locus_tag="PLW82_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46907.1"
                     /translation="MNRAVRLGAAVGLLTLSYINATEAEVALSLWIASFLVFAVTALL
                     DDKWQYLTVLLSLPLLYALYTSGSRDALTLAAFGLFSLLSFKQIALGFWALAAVLFLA
                     EGFFAEWHLNTLPFLLLGLAALSAEGRSKAFLPAAYSALYGGPSPEETAQLKRENAYL
                     RGQLEKARGPKGDQPTGFGRELKEITSNLAVLLFEVSCLVEESVLRSIPVFAELKDQL
                     QTLQKRVPEAFSEGFFKEPLSAVLDGIALEKEAVFHLKLKKTDLTAGEKFVMGELTKT
                     LVEKGKEYEITLSDGKEGLLYTVRPAVKKQLDERVEKLVDGIKGSVIFGDGSAYLIWR
                     TETHEGTLDSTA"
     gene            complement(2317..>2704)
                     /locus_tag="PLW82_06240"
     CDS             complement(2317..>2704)
                     /locus_tag="PLW82_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46908.1"
                     /translation="KPHIAWIIVGVMSVGYAVFLYFDAFSKKDYRSPVMYTLLSFSVP
                     ITLQKHLRNVGEGYDVLLVLCYILFMTIIPAVVYAIGVVVDLIGQLNLPDQEIAGPIE
                     PDTKKPEEERKTKNDDESLGPFRFDK"
BASE COUNT          746 a          691 c          592 g          675 t
ORIGIN      
        1 atcttcgtag accagtgcta aagcaaagcc gatggggatg acaaagaaca agcctgtaac
       61 catctgctcg atcccgaaaa agaccgccac caagatcaaa agggcaagcg agaaagcggt
      121 aagatatctg tagccatact tggtcttgat caccgccatc atcccgggaa tcaggtactt
      181 taagtaggtg ccaaaagttg gaaacaggac gccaacgaaa cacagcacta ggctaacggc
      241 aagaagcaat ccagttgtag ttagctcgga ggccttggct cttgtgtcca taaccgcact
      301 ctccctttaa tgaggcaaag tctagttcaa gaagtctcta tttaaagagt ttcccaaaag
      361 ccagcaaaaa acctccaagg ccaacctgta gatagaagat ttttccgtaa ccgccttcca
      421 gaggctcggt tggcagaaga aaacggtagg cagaactgaa gtttctgcct accttattcc
      481 acaaacccgt accggttcat ctcaagctcg gaaagggcga gcctgaagtt ttctgcatca
      541 agagtcgcat atccgtcgat gcctagagaa agcagctgcc agagaaaacc atctgttgga
      601 aagtctacaa catagagtag cctagcctct gttgcctcct taaggactcc aaacccttta
      661 acaacgtctg ctgcaacctc cggagtgaac acgatcagat cttcaccctg ccccaaaaac
      721 cttttagctg catcgaagct tccggcagac ttagccagat gcagatccct gcgaaggagc
      781 ttaagcagag cttcggcttt tctatgcggt gctatcaaga gtaccctcat gcgtttccgt
      841 cctccaaatc aggtaagcgc tgccgtcgcc gaagatcaca gatcccttga tgccgtcaac
      901 cagcttctct actctttcgt cgagctgctt ttttaccgct gggcgtactg tgtacagcag
      961 cccttccttc ccatctgaaa gggttatctc gtactctttg cccttttcta caagggtttt
     1021 agtcagttcc cccatgacga atttctcccc tgcggtgagg tcggtcttct ttagcttcag
     1081 gtggaaaaca gcttccttct ctaaggcaat gccatctaga acggcgctta atggttcttt
     1141 aaagaaccct tcggaaaaag cttcgggaac tctcttctga agcgtttgca gctgatcttt
     1201 cagttctgca aacaccggga tgcttcggag aacactttct tccacaaggc agctcacctc
     1261 aaatagcagc accgcaaggt tgctggtgat ctctttcagc tcccgaccaa agccggttgg
     1321 ctggtcaccc tttggtcctc ttgctttttc cagctgtcct ctgaggtagg cattctctcg
     1381 cttcagttgt gctgtctctt caggtgaagg accaccataa agtgcagaat atgccgcagg
     1441 caaaaaagcc ttggacctac cctctgcaga aagagcggca agtcccaaaa gaagaaatgg
     1501 tagggtattc agatgccatt cggcaaaaaa gccttctgca agaaacagca ctgccgccag
     1561 tgcccagaag cctagagcta tctgtttgaa gctcaaaagg ctgaaaaggc cgaaagcggc
     1621 gagagttaaa gcgtctctgc tccctgaagt gtagagggcg tatagaaggg ggagcgagag
     1681 gagtaccgtc aggtactgcc acttatcatc taagagagcg gtcactgcaa acaccaggaa
     1741 agatgcgatc cataaagaca atgccacttc tgcctccgtg gcgttgatat aggagagagt
     1801 caaaagaccc actgctgctc caaggcgcac tgctctgttc atctcattca cctctagctc
     1861 gtagacatgt ttactactaa taggtatgtt tgtctgatga actttaggat agtaagtggc
     1921 aaaaccttaa aaggagcata ccgttttttc aaatcagctt ccggacaagc gatttgcact
     1981 agttctggca atcgggtcga ggacttccca ttaattgagt aaccgatctt tgtgcgcaag
     2041 cgctcgttcg ggcatgggaa ggggcgggcc aaacaattag cccgcccccg ccttttagag
     2101 aagtcctttt tcgctttcct gcaccaaaaa aacagaccga gttactgcga ttccaagtgt
     2161 tcttggagtc ttgggtgaca tactagcctc gtttatcact tttacttctc gaaaactgat
     2221 ttgctccggc caaacgattt gagttgtgtt ttcaaagatc tccttttaca acgacgaaaa
     2281 ctcaaggtta ttgttaccag tcttttcaac agagctttat ttatcaaacc ggaaaggacc
     2341 taaagactcg tcgtcgtttt tggtttttcg ctcttcttcc ggtttttttg tatccggttc
     2401 aatcggacca gctatttctt gatccggcaa gttcaattga ccaatgagat caacaacaac
     2461 cccgatagca tacacgacag ctggaataat cgtcatgaac aagatgtaac aaagaaccaa
     2521 cagaacatca taaccttctc caacattccg caaatgtttc tgtagtgtta taggtacaga
     2581 aaaactcaaa agcgtataca tgaccggaga ccgatagtct tttttagaaa aagcatcgaa
     2641 atacaagaaa accgcatacc caacagacat aacacccaca ataatccatg caatatgtgg
     2701 ttta
//
LOCUS       DAOSDA010000251         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_24504_length_2698_cov_2.980704, whole genome shotgun sequence.
ACCESSION   DAOSDA010000251 DAOSDA010000000
VERSION     DAOSDA010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24504_length_2698_cov_2.980704"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLW82_06245"
     CDS             <1..558
                     /locus_tag="PLW82_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HOJ46909.1"
                     /translation="AVDYAFGRWRDDAGVIGVASELERQLTMLGIGVYRVKKIHDGEV
                     FRLAYVYSEESVKEALNRYPHAKLVIDIHRDAAPDSQPFRIPIDGQYAAQVSLVVATG
                     ERSSRSWDVALTRSLASKLISMINSRYPGLCQGAIFKTGAYNQHLHPGSLLIEVGNDC
                     NTDAEAQYTARLLAPIIRDLLMEVR"
     gene            559..978
                     /locus_tag="PLW82_06250"
     CDS             559..978
                     /locus_tag="PLW82_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46910.1"
                     /translation="MYFTTHLSCGLFCGALTKNSLGALCLGLVSHAALDMIPHHDYDS
                     VGRAAVDIASGLVILSAMQTLFPGLSGPLLWGAIGGTIPDLEVALRRIFPGYKLRRLF
                     PSHSGLLPHPQREFPKGVLVQFAIIAVCLFFCFSLGR"
     gene            1024..2358
                     /locus_tag="PLW82_06255"
     CDS             1024..2358
                     /locus_tag="PLW82_06255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOJ46911.1"
                     /translation="MKTHRFRLYSSILLFLALAKLAVVSKPLTLYAPENLISVAQVVA
                     KQNGLILTPIASSADYWLTLDGGRTIGREPVAVCIDAGTHIEVPILDKSPLLQSVPVD
                     GYLPHEAGYPKYREINLVTRPLSLLEKLWQGIRILKVGRTMSRVALDLQPTVVALAGD
                     IMLGRRVGEKILEKGPEYPFAKVYSVLQAADITFANLEAPLCITGEFINLFRAHPAAV
                     AGLKLAGIDVVSLANNHILDYHQEGMLETWEHLNRAGILQVGTGMNLQEAVQGAVIDA
                     NGMKVGFLAFTETWFLYSRKGIEWVAGSCPGVAPIDLELIVSNIKRFKQAGVQVVIVS
                     LHWGVEYSQTVAEKEAELAKAIIDAGGDVVVGHHPHVVKPWGFYKGKPVFFSLGNFIF
                     DTLRPPLSEQGLLVELVYLEGLRDVHLTVTRLNDCQVELTKLPPPEANVIIN"
     gene            2388..>2698
                     /locus_tag="PLW82_06260"
     CDS             2388..>2698
                     /locus_tag="PLW82_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOJ46912.1"
                     /translation="MQAKHQDLIRNFCIIAHIDHGKSTLADRLLEITNTVSEREMKEQ
                     LLDSMELERERGITIKAKAIRMNYSAKDGKVYQLNLIDTPGHVDFSYEVSRSLAACEG
                     AL"
BASE COUNT          657 a          646 c          725 g          670 t
ORIGIN      
        1 gcagtggatt acgctttcgg ccggtggcga gacgatgcag gggttattgg ggttgctagt
       61 gaactagaaa gacagctgac aatgctcggc attggcgttt acagagtaaa aaagattcat
      121 gatggagagg tctttcgtct ggcgtatgtc tacagcgaag agtctgtgaa agaagcgctg
      181 aacaggtatc ctcatgcgaa gctggtcatc gacattcacc gagatgcagc tccggactcc
      241 cagccttttc gcatacccat tgacgggcag tatgcggcac aagtgtcttt ggttgtcgct
      301 acaggggagc ggagtagcag aagctgggac gtagcgctca cgcgcagtct tgcttccaaa
      361 ctcatcagca tgataaactc cagatatccc ggtctgtgcc agggagctat cttcaagacg
      421 ggagcttaca accagcatct gcatccgggg tcgctgctca ttgaggtggg taacgactgc
      481 aataccgatg ccgaagccca gtacacagcg aggctcctcg ctccgatcat cagagatctt
      541 ttgatggagg tgcgctaaat gtatttcact acccatcttt cctgcgggct tttctgcgga
      601 gctcttacta agaactcact tggagcgctc tgccttggac ttgtgagcca tgcagctttg
      661 gacatgatcc ctcatcacga ctacgattca gtaggaaggg cagcagtaga tattgcttct
      721 ggtcttgtaa tcctttcagc catgcaaacc ctttttccag gtctttccgg tccgctcctt
      781 tggggggcca ttggagggac tatacccgat ctagaggtgg ctttgaggcg aatctttccc
      841 gggtacaagc tcaggcgcct tttcccaagc cacagcggac ttctacccca tcctcagagg
      901 gagttcccca aaggcgtact tgtgcagttt gccatcatcg ctgtttgtct gtttttttgc
      961 tttagtttag gcagatagtg ctaattccac cgcccggcgc gtacaagtaa cgtgggtgat
     1021 cgtatgaaaa cgcacagatt tcgcctatac agcagcattc tgcttttttt ggctctggca
     1081 aagctagctg ttgtatccaa gcccttgact ttatacgctc cggagaacct gataagtgtg
     1141 gcacaggttg tggcaaagca aaatggacta attctcacac cgattgcctc cagtgcagac
     1201 tactggctta cccttgatgg tggcagaact atcggcagag aaccggtggc cgtctgtata
     1261 gatgcaggca cccacatcga agttcccata ctggacaaat caccgcttct ccagtccgtg
     1321 ccagttgacg gttacttacc ccacgaagct ggttacccga aatacagaga gatcaacctc
     1381 gtgacaaggc ccctttcgct tttggaaaag ctgtggcagg gaattagaat cttgaaagtt
     1441 gggcggacca tgagtagagt tgccctggat ctccagccta cagttgtggc gcttgcaggc
     1501 gacataatgc taggaagacg tgtaggtgag aagatccttg aaaaagggcc ggaatacccg
     1561 tttgccaaag tgtatagcgt cttacaagct gcagacatta cctttgccaa cctagaggca
     1621 ccgctttgta taacaggaga gttcatcaat ttatttcgcg cccatccggc agcagtggcc
     1681 ggtttgaagc ttgcaggtat cgatgtggtt agcctcgcca acaaccacat tttagactac
     1741 catcaggaag gtatgctgga aacctgggag cacttaaatc gagcgggaat tctccaggtg
     1801 ggaaccggta tgaaccttca ggaagcggtg cagggagcgg taatcgatgc aaacggaatg
     1861 aaagtgggct tcctggcttt taccgaaacg tggtttctct actccagaaa gggcattgag
     1921 tgggtggcag gcagctgtcc cggcgttgct cctattgatc tggagttgat tgtctccaac
     1981 atcaaaaggt ttaagcaggc cggggttcag gtggtgatag tcagccttca ctggggagtg
     2041 gagtattcgc agactgtcgc cgagaaagaa gcagaacttg ccaaagccat catcgatgca
     2101 ggaggagacg tggttgtggg gcaccatcct catgtggtta aaccttgggg tttttataaa
     2161 ggaaaaccgg ttttttttag tctggggaac ttcattttcg atacgttgag accgcctctt
     2221 tcggagcaag ggctcctggt ggaactggtg tacctcgaag ggcttagaga tgtgcacctc
     2281 actgtaacta ggctcaacga ttgtcaggtc gagcttacca agttgccccc acctgaagct
     2341 aatgttataa ttaattgatt gaagattcag ggggtgcctg agataaagtg caagccaagc
     2401 atcaagattt aattcggaac ttttgtatca ttgctcatat cgaccacggc aaatccacgt
     2461 tggccgacag gcttttggag attaccaata ctgtctcaga gagagaaatg aaagagcagc
     2521 tccttgattc tatggaactc gagagggaaa gaggcattac catcaaagcg aaagccattc
     2581 gcatgaacta cagtgctaaa gatggcaaag tctaccagct caacctgatt gatactccgg
     2641 gacacgtgga cttcagctac gaagtctcgc gctcgctggc agcctgtgag ggcgcgct
//
LOCUS       DAOSDA010000252         2691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_24589_length_2691_cov_2.849014, whole genome shotgun sequence.
ACCESSION   DAOSDA010000252 DAOSDA010000000
VERSION     DAOSDA010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24589_length_2691_cov_2.849014"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..993
                     /locus_tag="PLW82_06265"
     CDS             61..993
                     /locus_tag="PLW82_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ46913.1"
                     /translation="MKKLQWMISLIFIISLVACAVDIGIIQIIEHPALDAARRGFMDE
                     LEARGVKANYSYHNAQGDIATLYTIAGNIVASKVDLILAIATPSAQAAANATSEIPIL
                     ITAVTDPQNAGLVKDFDKPGTNVTGTSDMNPVEKQLELVKRLVPKASSVGIIYNAGEA
                     NSVVQIEIAKKAAEKLGLKLVLVTVSETSGVFQAAQSLQGRVDAVYVPTDNTVVAALE
                     SVIAFCERNKIPLIAGETESVARGALATIGLDYYELGRQTGAMAVEVLNGANPAETPI
                     QFQETAILMVNKSAAQRMGITIPQDLLEEAEVVE"
     gene            1064..2035
                     /locus_tag="PLW82_06270"
     CDS             1064..2035
                     /locus_tag="PLW82_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ46914.1"
                     /translation="MLGIWQAPLEQGLAYGILVLGVYLTFRTLNHPDLTIDGSFPLGA
                     AIAASAIIKGIHPLFATLLGFVGGFVAGMVTGVLNTKLKISGLLAGILTMTGLYSVNL
                     RIMGGRSNIPLIRETTLVTMAQDTMGMAWGTVVLFALIVAFIKLVLDYFLHTEMGLGL
                     RACGDNEKMIKALGVDTDFYRIFGLAMANGLVGMAGSLVAQYQGFADVGMGIGTAVAG
                     LASVILGEAILRPKTIFTATLSAVFGSVLYRFTITLALRAGFAATDLRLVTAVLVIVA
                     LALPKLKETKLAKKVFDIKKQAEVQPAEVVRREANPITTLVTSLIRK"
     gene            2041..>2691
                     /locus_tag="PLW82_06275"
     CDS             2041..>2691
                     /locus_tag="PLW82_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ46915.1"
                     /translation="MLLMERVNKTFELGGGTLKRALVDVNLVANEGDFITIVGSNGAG
                     KSTLLNMVAGVYLPDSGKIRVNGSEITNWPEHKRARYIGRVFQDPMMGTAGSMTIEEN
                     LAMAFKRGQRRGLRLGVGRNRAKFRAVLEELGLGLESRLKDPVRLLSGGQRQSLTLMM
                     ATLTAPKILLLDEHTAALDPKTAATIIELTKSIVENHSLTVLMVTHNLKQALEVGNR"
BASE COUNT          720 a          658 c          715 g          598 t
ORIGIN      
        1 gttatgaaag tttacaaaga aaggtacagc tggaaaggca tttgaggagg ttcctagtcg
       61 atgaaaaagc ttcaatggat gatttccctg atctttatca taagtcttgt tgcttgtgcg
      121 gtggatattg ggattataca gattatcgag catcctgctc tggatgctgc aagaagaggc
      181 tttatggatg aactagaagc caggggagta aaagccaact actcttacca caacgcccag
      241 ggcgacatag caactctgta caccatagcc ggaaacatag tggctagcaa agtagatctc
      301 attctcgcca ttgctactcc ctcggcacaa gcggcggcca acgcaacttc ggagatacct
      361 atcctcatca cagcggtaac agatccgcag aacgcaggct tagtcaaaga tttcgacaag
      421 ccaggcacca acgtcaccgg tacctccgac atgaatccgg tagaaaagca gttagagctg
      481 gtcaaacgcc tcgtccctaa agcttctagt gtgggcatca tttacaatgc cggagaagcc
      541 aactcagtag tgcagatcga aattgcgaaa aaagctgcag aaaaactggg actcaagctc
      601 gtattggtaa ccgtaagcga aaccagcggc gttttccaag cagctcaatc tctacagggt
      661 agagtagacg cagtctacgt acctacagac aatacggtag ttgctgctct cgaatcggtt
      721 atcgcgttct gtgaaaggaa caagattcct ctgatcgcag gtgaaaccga atcggttgcg
      781 cgcggagctt tagcaacaat cggacttgac tactacgaac tgggccgcca gaccggggct
      841 atggcagtgg aggtgctaaa cggagcaaat cccgccgaaa cgccgattca gttccaagag
      901 acagccattt tgatggtaaa caagtcggcg gcacagagaa tggggattac gatcccacaa
      961 gatcttttgg aagaagcaga agtggtagaa taacctaaac caaggttact ttacctggaa
     1021 atggaggtga acagcctgca agctttttgc aggctgtgca aatatgcttg gaatttggca
     1081 ggcacccttg gaacaagggt tggcatatgg aattttagta ctaggagtct atctcacatt
     1141 ccgcacgctg aatcacccgg atctgaccat cgacggcagt tttccgttag gcgcagcgat
     1201 tgcggcatct gcgatcataa agggtatcca tccgctgttt gccaccctcc tgggctttgt
     1261 tggaggcttt gttgccggaa tggttacggg cgttttgaac accaaactga agatttcggg
     1321 gcttcttgca ggtatcttga caatgacagg actctactca gtgaacctgc ggatcatggg
     1381 tggaagatcc aatattcccc tgatcagaga gaccactctt gtaacaatgg cccaggacac
     1441 catgggtatg gcgtggggca ccgtcgttct ttttgcactt atagtggcat ttataaagct
     1501 cgttctcgac tacttcctcc ataccgagat gggcctcgga ctgagagcct gcggcgacaa
     1561 tgaaaaaatg attaaagctc ttggtgtgga caccgacttc tacagaattt ttggcctggc
     1621 catggccaac ggattggtag gcatggcggg aagcctagtg gcccagtacc agggttttgc
     1681 cgatgtaggt atggggatcg gcactgcagt tgcgggtctg gcatcagtaa tcttgggaga
     1741 ggccattctc agacccaaga ccatcttcac tgcgaccctt tctgcagtct tcggatcggt
     1801 actctaccgc ttcaccataa cccttgccct acgagcgggt tttgctgcaa ccgacctgcg
     1861 cctagtcaca gcagtgctgg tcattgttgc actggcccta cctaagctga aagagaccaa
     1921 gcttgccaaa aaggtgttcg atataaaaaa acaggctgaa gtacagccgg cagaggtcgt
     1981 taggcgggaa gccaatccca tcacaacact ggtgacctct ctgattagga agtgaagacc
     2041 atgctgctta tggaaagagt caataaaacc tttgagctcg gcggtggcac tttgaagagg
     2101 gcgttggtag atgtaaacct agttgccaac gagggcgact tcatcaccat cgttggttct
     2161 aacggtgccg gcaaatctac gctgctcaat atggtagcag gagtatacct ccctgattcg
     2221 ggcaagatca gagtgaatgg ctccgaaatc accaactggc ccgagcacaa gagggcaagg
     2281 tacatcggca gagtcttcca agaccccatg atgggtacag cgggatcgat gaccatagag
     2341 gagaacctgg cgatggcctt caaacggggg cagaggagag gactacggct gggagtaggc
     2401 agaaaccgag ctaagttccg tgctgtactt gaagaactgg gactggggct tgagagccgg
     2461 ctcaaagatc ctgtacgcct cctttcagga gggcagaggc agtccctgac gttgatgatg
     2521 gccactctaa ctgcaccgaa gattctcctt ctcgacgaac acacagccgc gctggatccc
     2581 aaaaccgctg cgacgatcat cgaacttacc aaaagcatag tagaaaacca cagcctgacg
     2641 gtgcttatgg taactcacaa cctcaagcaa gcccttgaag tgggcaaccg g
//
LOCUS       DAOSDA010000253         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_24848_length_2670_cov_3.700956, whole genome shotgun sequence.
ACCESSION   DAOSDA010000253 DAOSDA010000000
VERSION     DAOSDA010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24848_length_2670_cov_3.700956"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /gene="metK"
                     /locus_tag="PLW82_06280"
     CDS             complement(<1..878)
                     /gene="metK"
                     /locus_tag="PLW82_06280"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOJ46916.1"
                     /translation="MAERYLFTSESVTEGHPDKIADQISDAILDAIMAEDPLSRVACE
                     TLLTTGLVFVSGEITTNCYVDIPTIVRSTLLDIGYTRAKYGFDGETCGVITSVHEQSP
                     DIAMGVDEALEVRGQGQKGVELGAGDQGIMFGYACDETPELMPMPISLAHKLAKKLSE
                     VRKNGKLPYLRPDGKSQVTVEYEDGIPVRVDTVVISAQHNPEITHNELADDIIQFVIK
                     EVIDRKLLDEKTNFLINPTGRFVVGGPQGDTGLTGRKIIVDTYGGAAPHGGGAFSGKD
                     PTKVDRSASYAARYVA"
     gene            complement(883..2094)
                     /gene="coaBC"
                     /locus_tag="PLW82_06285"
     CDS             complement(883..2094)
                     /gene="coaBC"
                     /locus_tag="PLW82_06285"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOJ46917.1"
                     /translation="MGNIFKDKVVLIGVTGGIAVYKTVELVSALRKRGAQIRIIMTEN
                     ATRFVAPLTFREVGNAPVYYDMWHEPVHYDMAHISLAEEADVCIVAPATANFIGKFAS
                     GIADNLLGTTLLATEAPIAIAPAMNTHMWANPAVQDNVAKLKSRGIKVIEPDAGHLAC
                     GQVGKGRLPSVDTLLFELESMLWPQDLTGKRFLITAGPTREFIDDFRFISNPSTGKMG
                     LALAKAVKLRGGDVDLVLGPCEISVPRDISVHNVSSALDMYETARSLFANCDVFISAA
                     AVSDFRAEKRFSGKMKRSMTDDVWSLPLVHNPDILGSLSEEKKHQVMVGFAAESSEIL
                     ANAKKKLEDKGLDMIVANDISRADTGFAADTNSVWLLGRDFEVEIGLESKEAIAHKIV
                     DKIVEKLLVRK"
     gene            complement(2110..2373)
                     /gene="rpoZ"
                     /locus_tag="PLW82_06290"
     CDS             complement(2110..2373)
                     /gene="rpoZ"
                     /locus_tag="PLW82_06290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOJ46918.1"
                     /translation="MRNQPYLDDLEEILPSRYTMVIAIAKRARQIMQEEREQDENGYT
                     GITKPVSIAMYELLDRKFEVTEKPFEKKEIVPKSQIQDFSDFE"
     gene            complement(2366..>2670)
                     /locus_tag="PLW82_06295"
     CDS             complement(2366..>2670)
                     /locus_tag="PLW82_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOJ46919.1"
                     /translation="KQVVEKWPDAVSIFLIPPSMEELERRIKNRRTETSEEIEARLAQ
                     SLIEMNEGRMYDYYLVHDNTEHTVLEFEAIRLAESFKVNRTDWDRVIDAVKGDFNA"
BASE COUNT          623 a          693 c          600 g          754 t
ORIGIN      
        1 ttagcaacat atctagcagc atagctagca gaacggtcga ctttggtggg gtctttgccg
       61 gaaaaagctc cgcctccgtg aggagctgcc ccgccgtagg tgtcgacaat gatctttctg
      121 ccggtcagtc cggtatctcc ctgaggacct cccactacaa aacggccggt gggattgatc
      181 aggaagttgg ttttttcatc cagtagtttg cggtcgataa cttctttaat aacgaactga
      241 ataatatcgt cagcaagttc attatgggtg atttccggat tgtgctgggc ggaaatcact
      301 acggtgtcca cccttacagg tataccgtct tcatactcaa cagttacctg gctctttcca
      361 tccggtctga gataagggag cttgccgttc ttccttactt cgctcaattt cttggccagt
      421 ttgtgggcta aagaaatggg catcggcatt agttcagggg tttcgtcgca agcgtagcca
      481 aacataatac cctggtcgcc tgcacccagt tcaacgcctt tctgtccttg tccccttact
      541 tctagtgctt catccacacc catggctatg tccggcgact gttcatgaac cgatgttatg
      601 actccgcatg tttcgccgtc aaagccgtac ttggccctgg tgtagcctat atctaagagt
      661 gtacttctga ctattgtcgg tatgtcaaca taacaattgg ttgtaatctc tcccgatacg
      721 aaaacgagcc cagtggtcag taaagtctca caggctacgc ggctgagtgg gtcctctgcc
      781 atgatggcat ctaatatcgc atccgaaatc tggtcagcaa ttttgtccgg atgaccttcg
      841 gtaaccgact cagaggtaaa taaataacgt tcagccattt gttcacttcc ttaccaaaag
      901 cttttctact atcttgtcta ctattttgtg tgcaatagcc tccttagact ctaagcctat
      961 ctccacttcg aagtctctgc ctaaaagcca aacagagttg gtgtctgctg caaacccggt
     1021 gtctgcgcgg ctgatgtcgt ttgccacgat catatcgagt cctttgtctt ccagcttctt
     1081 tttagcgttt gccaggatct cgctggactc tgcagcaaat cccaccatta cttggtgttt
     1141 cttctcttca gacaaagatc ccaaaatgtc cggattgtgc actagaggaa gtgaccatac
     1201 atcgtcggtc atgcttcttt tcatcttgcc ggaaaaacgt ttttcggcac ggaagtctga
     1261 aactgctgcg gcactgatga acacgtcgca gtttgcaaat agagacctgg cagtctcgta
     1321 catgtcgaga gcgctggaaa cattgtggac ggagatgtcc cgaggaacgc taatctcgca
     1381 gggtccgaga acaaggtcta cgtcaccgcc cctgagcttt acagccttgg ctaatgccag
     1441 tcccattttc cccgtagagg gattggagat gaagcggaaa tcgtcgataa actcccttgt
     1501 tgggccagca gtaatgagaa acctttttcc ggtaagatcc tgaggccaga gcatgctctc
     1561 caactcgaaa aggagagtgt caacgctggg cagacgacct ttgcctacct gcccacaggc
     1621 cagatgcccc gcatcaggtt cgattacttt aattccacga cttttcagct tggcaacgtt
     1681 atcctgaact gcgggatttg cccacatgtg agtgttcatt gctggagcaa tggcaatggg
     1741 ggcttccgtc gcaaggagag tagtccccaa gagattgtcg gctatgccac tggcaaactt
     1801 gccgataaag ttggcggttg caggagccac gatacagacg tcagcttctt ctgcaaggga
     1861 tatatgggcc atatcgtaat ggacaggttc atgccacatg tcatagtaga cgggtgcatt
     1921 acctacttct ctgaaggtaa gaggcgccac aaagcgggtt gcgttttcgg tcatgatgat
     1981 ccgaatctgc gctccccgtt ttctcaaggc actcaccagt tcaaccgttt tgtaaacggc
     2041 gatacccccg gtcacaccaa tgagtacaac cttgtcctta aagatgtttc ccatagttag
     2101 gccccctgtt tactcaaaat cgctaaaatc ctgaatctga ctcttaggca caatctcctt
     2161 tttctcgaag ggtttttcgg taacttcaaa cttcctatcc aaaagctcgt acatggcaat
     2221 ggatacgggt ttcgtaattc cagtatagcc attttcgtct tgttctcttt cttcctgcat
     2281 gatctgcctt gctcttttcg caatggcaat gaccatagtg tacctgctag gcaaaatctc
     2341 ctctaaatcg tccaaatatg gttgattacg cattgaaatc cccctttaca gcatcaatta
     2401 cccggtccca gtcggttcga ttcaccttga aggactcagc caatctgata gcttcaaact
     2461 ccagcaccgt gtgttccgta ttatcatgaa caagatagta gtcgtacatc cgtccctcgt
     2521 tcatctcaat aagagattga gcaagtctcg cctcaatctc ttcgcttgtt tcggttctgc
     2581 ggtttttgat gcgtctttct aattcctcca tagaaggtgg gatcaaaaag atcgaaacgg
     2641 catcaggcca tttctctacc acttgtttag
//
LOCUS       DAOSDA010000254         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_24856_length_2670_cov_2.902103, whole genome shotgun sequence.
ACCESSION   DAOSDA010000254 DAOSDA010000000
VERSION     DAOSDA010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24856_length_2670_cov_2.902103"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLW82_06300"
     CDS             complement(<1..926)
                     /locus_tag="PLW82_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ46920.1"
                     /translation="MIESFRFFGFLFRPGAITKKTLSDLPYFRLNAEGKDKPISYEIS
                     FFLNGVRYRYGIEVDSDGICSEWLYFVPKRQEACYFERDRDQITRTGTYFKDKKALQY
                     IKPDSTRPFLFTLAQDSVRKFDWAKSIVDYFRFYIRSSDMPETFFKNMMDDEIACKSD
                     SGIISKDEVVSYLRLADSGITDVSVIEKNAEDDELIEQLTEFVKQRGAKIEFERERWE
                     TFFHHAKYDNDKLVGDEKFPLRAESRGTFKLYCMLYPILCVLKKGGVLLVDEIESSLH
                     PLLCEKIVQVFNSSKTIKSISVCKIPRLDELSV"
     gene            complement(1479..1886)
                     /locus_tag="PLW82_06305"
     CDS             complement(1479..1886)
                     /locus_tag="PLW82_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46921.1"
                     /translation="MEKLFGIFAFIMLLFVFSALTITTEYLTPFGTVTVSVSPTVFEW
                     TVSVNADDYRTESYFEVHSPGFYVLVTDGHAPKGQFTVRILSSSNRVVYEAQAAHVHP
                     QNKIYLEPGRYLISVTSKNAKHTSYTIYLTGAD"
     assembly_gap    1992..2001
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2002..2489
                     /locus_tag="PLW82_06310"
     CDS             <2002..2489
                     /locus_tag="PLW82_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HOJ46922.1"
                     /translation="HKKDGVAVGKRFKTFRGFETARSCKGKLSASLEDYLEMIYRRTR
                     SGELLKPADLAKELSVSPSAITKNVQRLACLGFVTYEPYGKIQLTDLGAKTGEFLIQR
                     HEIIEEFFAVLGSGEIGFLETEMIEHYLSPATVERLEQLTAFLRHHHRLWREYLDEKK
                     L"
     gene            complement(2508..>2670)
                     /locus_tag="PLW82_06315"
     CDS             complement(2508..>2670)
                     /locus_tag="PLW82_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HOJ46923.1"
                     /translation="LNHFPCGTTLLTIKKETGSWKWTALAFIIPTLVGIVGCILVAQI
                     YNFFSFLLL"
BASE COUNT          735 a          594 c          604 g          727 t
ORIGIN      
        1 acgctgagtt catctaaccg agggatttta cacacactta tggacttgat cgtttttgaa
       61 ctgttaaaaa cctgtactat cttctcgcac aaaagcggat gcagtgaact ttcaatttca
      121 tctaccaaaa gaactccacc ctttttcagc acgcagagta tcgggtagag catacagtat
      181 aatttaaatg ttcccctcga ttctgcacgc aacgggaact tctcatctcc aactagcttg
      241 tcattgtcgt atttagcatg atggaaaaac gtctcccatc tctctctctc aaactcaatt
      301 tttgcacctc gttgcttcac aaactcagtt agctgttcga taagctcgtc atcctctgcg
      361 tttttttcta ttactgaaac atctgtaata ccggaatctg ccaatctcaa ataagacact
      421 acttcgtcct tggagatgat gcccgaatca gacttgcaag caatctcgtc atccatcatg
      481 tttttgaaga atgtttctgg catgtcactt gacctgatgt agaacctgaa gtagtccaca
      541 atactctttg cccaatcgaa cttcctaaca gagtcctgtg cgagggtaaa caagaacggt
      601 cttgttgaat cgggttttat atactgcaat gcttttttgt ctttgaagta tgtcccggtg
      661 cgagttattt ggtctcgatc tctttcaaag tagcatgctt cttgcctctt tggaacaaaa
      721 tacaaccatt ctgaacatat gccgtcactg tcgacctcaa tgccataacg gtaacgaaca
      781 ccattaagaa aaaacgagat ctcgtacgat atgggtttgt ccttaccctc tgcgttaagc
      841 ctaaaatacg gcaaatcgga aagagttttt ttagtaattg ctccagggcg aaaaagaaag
      901 ccgaaaaatc taaacgactc tatcaaattc gatttcccgc ttgcgttgct tccgtacaga
      961 gcagcaacct ttaataagtt cgtgtctccg acttggaatg tgtttgtatc tgctaactcc
     1021 tgttcttatc tgcaaccatc gaaagaacaa cttcatccct aaaagatctg aagttcttaa
     1081 ccgtgtactc aattaacata agagcagcct ccttgttagt tatatggtaa cattttttga
     1141 ggtactagca agatttgtgc gagaatctcc cattttacgc acctccacta tctttttccc
     1201 taatcaatag tatgtcttcg tggttcaata atattccagg cgtgcaaaga ctaggaagac
     1261 agcaggcaga agaagatggt acattcttgt cataacagag ttcagtttaa aagcgttgtg
     1321 cgtgcacctg tacctaaagg ctcaaagcgt gcaaacagaa agggctgctg cacaactagc
     1381 cgattgtaca gcagcctctt ttttgtcgag atagtagaaa gggagtgttg ctaaactact
     1441 tgtgtagtag tcttgcaaca ctcccatatg agtctaggtc agtctgcacc agtgaggtag
     1501 atggtgtagc tggtgtgttt ggcgtttttt gaggtgacgc tgatcaggta gcgtccgggc
     1561 tctagataga tcttgttttg cggatgcacg tgagcggctt gagcctcata aaccacgcgg
     1621 ttggaggaag acaggatgcg gacggtaaac tgacctttcg gtgcgtgtcc gtcggtaacg
     1681 agaacataga agcctgggct gtgcacctca aaataacttt cggtgcggta gtcgtcggca
     1741 tttacagaca ccgtccactc aaagaccgtc ggagaaacgg aaacagtcac ggtcccaaac
     1801 ggcgtgaggt attctgtggt aatggtcaga gccgaaaaaa caaaaagcag catgataaac
     1861 gcgaagatac caaatagctt ttccatggcg cactcctttg ctcaaaagaa ataacttgag
     1921 aaacatttgg ttctagcata tctattaata tgcggcttga aaaagtttcc tgagttacat
     1981 cgcttttcat cnnnnnnnnn naccacaaga aagatggggt tgctgtgggg aaaaggttta
     2041 agacgtttag gggttttgag accgcaaggt cctgtaaggg gaagctttct gccagcctcg
     2101 aagactacct ggaaatgatc taccgcagaa ccagaagtgg cgagcttttg aaaccggcag
     2161 acctggcaaa ggagctcagc gtgagtcctt cggcgatcac caagaatgtg cagcgcttgg
     2221 catgtttggg ttttgtcacc tacgagccct acggcaagat ccagctcaca gaccttggtg
     2281 caaaaaccgg tgagtttctc atccaaagac acgaaatcat cgaggagttc ttcgctgttt
     2341 tgggtagcgg cgagatcggt tttttggaaa cggagatgat cgagcactac ttgagtccgg
     2401 ctaccgtaga gcggctcgag cagcttacgg catttctaag gcatcaccac aggctgtgga
     2461 gggagtatct tgacgagaaa aagttatagg agatctctag ggatctccta taacaacaaa
     2521 aaggagaaaa agttgtagat ctgtgcaacc aggatacagc cgacaatacc cactagtgtg
     2581 ggaatgataa atgccaaagc ggtccatttc caacttcccg tttctttctt gatcgtcaaa
     2641 agcgttgtgc cgcaggggaa gtggttcagg
//
LOCUS       DAOSDA010000255         2663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_24955_length_2663_cov_2.840491, whole genome shotgun sequence.
ACCESSION   DAOSDA010000255 DAOSDA010000000
VERSION     DAOSDA010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24955_length_2663_cov_2.840491"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLW82_06320"
     CDS             complement(<1..676)
                     /locus_tag="PLW82_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ46924.1"
                     /translation="MGIFQTGLQYFFFYSGIAHVSGVKSAILSSIGNFFVVIFAHFIY
                     KNDKLTFAKAFGLLTGFLGIVIVNWTPEAAGMSWNFTLLGEGFMIMSGLTSTVATFGA
                     KRLSGKLNPALINAYQLFFGSLLLIGAGLPGGIQPHITFTPKFWALLIYSAFLSSAAF
                     SIWYNLLKYNKAGEITIYRFVIPISGSLLSALLVPGEKLTVGIVAALVLVALGIATVN
                     YFAGSNN"
     gene            complement(724..924)
                     /locus_tag="PLW82_06325"
     CDS             complement(724..924)
                     /locus_tag="PLW82_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46925.1"
                     /translation="MKINIWTNKYTAAVLAIIACVLWGSAFPVLKATYAELNLRPDDV
                     SGRLLLAGARFFLASVMTYIVV"
     gene            1094..>2663
                     /locus_tag="PLW82_06330"
     CDS             1094..>2663
                     /locus_tag="PLW82_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOJ46926.1"
                     /translation="MLGLNLEAHTLPKHKVLQMLGANPGAGLTEKEAGGRKARHGPNE
                     LVKAKKRTLIQKLVDQLSDFLILILVGAAFISCLVGEYIDALLIVSIVVVNAVFGLVQ
                     ESKADKALEALKDMSAPKAKVLRSGKKSQVDAKELVPGDIVYLEAGDSVPADLRLIET
                     SNLEIQEAALTGESVPSGKDADRVYPKETAVGDRKNMAYMSTIVTYGRGTGVVVATGM
                     QTEIGRIAHAISTIDTSPTPLQVKLNDLSKKIGIAVLVICGVVFLTGFLKSLESPLHL
                     FLTAIALAVAAVPEGLPAVVTVILAMGMTRMSRQNALVRKLLAVETLGTTTVICSDKT
                     GTLTENAMTVVKIYLPSGRFDISGIGYDPSGSFELTSGRRSGLNELLTAGVLCNDAIY
                     TNKDGRHTVIGDPTEGALLVAAAKGGLHKEDLEHLYPRVMELPFESSRKKMSTLHKTS
                     SSYRLFVKGAPDEILKNCTQVLLDGTITTLTPVLRRTIAREMETMSDQALRILGFAFK
                     DYVKLPKKLTPSDES"
BASE COUNT          738 a          663 c          672 g          590 t
ORIGIN      
        1 ggttattgct gccggcaaag tagttaaccg tggcgatccc aagggcaacc aaaaccaaag
       61 cagctacgat gcctacggtc agcttttctc ccggtactag cagagcagaa aggagcgaac
      121 cggagatcgg aatgacgaac ctgtaaatag tgatctctcc ggctttgttg tacttcagta
      181 gattgtacca gatggaaaat gcggcactgg acaaaaaggc cgaatagatc aacagtgccc
      241 agaacttggg tgtgaaggtg atgtgaggct gaatccctcc ggggagccct gcaccgatta
      301 aaagcagcga accgaaaaag agctggtagg cgttgatcaa cgccggattg agcttgcccg
      361 agagtctctt ggcaccgaag gttgccaccg tactggtaag accggacatg atcatgaaac
      421 cttcgccgag aagagtgaag ttccaactca tgccggcagc ttcaggggtc caatttacga
      481 taacaatgcc taagaatccg gtcaaaaggc cgaacgcttt ggcaaaggtc agcttgtcgt
      541 tcttgtagat gaagtgggca aaaatcacaa cgaagaagtt cccgatggaa ctcaaaatcg
      601 cagacttaac accgctcacg tgagcgatgc cgctgtagaa gaagaagtac tggaggccag
      661 tctgaaaaat ccccaaaaaa attaggggca gcacccactt cttggatatt gtcatctcac
      721 tcttcatacc actatatagg tcatgacaga cgccaagaag aacctggctc cggcaagaag
      781 gagcctcccc gagacatcgt cgggtctcag atttaactcg gcgtaggttg ccttcagcac
      841 ggggaaggca cttccccaca gcacgcatgc aattattgct aaaactgcag cagtgtactt
      901 gttggtccaa atgttgatct tcaattcttg tccctctttc agttctttta aatgctaagt
      961 tttgcattgt tttgctagat tagttcactc tctgctttgt actagccttt atgaaggaag
     1021 tacacttgtc ctttaatatg ttaccttacg ggagacagtt tacccaaatc ggcggcaaaa
     1081 aggaaggatg cagatgcttg gtttaaactt agaagctcac actttgccaa agcacaaggt
     1141 tttgcaaatg ctcggagcaa atcccggagc aggccttaca gaaaaggaag ccggaggccg
     1201 caaagctcgg cacggtccaa acgaacttgt caaagcaaaa aaacgcacgc tcatccagaa
     1261 gctcgtcgat cagctgagcg actttttgat cctcattctg gtaggagcgg ccttcatctc
     1321 ctgcctggtg ggcgaatata tagatgctct cctgattgtg tccattgtgg tcgtaaacgc
     1381 ggtcttcgga ctggtacagg aaagcaaagc agacaaagcc ttagaagccc tcaaagatat
     1441 gagcgctcca aaagcgaaag tgctgaggag cggaaaaaaa tcccaggttg atgccaagga
     1501 gcttgtccct ggagacatcg tttatctgga ggcgggagac agcgtccctg cagatctcag
     1561 gcttattgaa acaagtaact tggagatcca agaggcagct ctcaccgggg aatcggttcc
     1621 ttcgggtaaa gatgcagacc gagtctatcc caaagaaacg gcagtaggcg atcggaagaa
     1681 catggcctac atgagtacaa ttgtaacata cggaagggga actggagttg ttgttgctac
     1741 gggaatgcag acggaaattg gcaggatagc ccatgctata agcacaatag acacatcccc
     1801 tactcctctg caggttaaac tcaacgacct gagtaagaag atcggtatcg ctgtgcttgt
     1861 aatctgcggc gtcgtgtttc ttacaggttt tctgaagtct ttggaaagtc ccctgcacct
     1921 gttcctgact gccattgccc tggcagttgc agcggttcca gaaggcttgc ctgcggtagt
     1981 caccgtcatc ctcgctatgg gcatgaccag aatgagcagg caaaacgccc tagtacgtaa
     2041 gctacttgca gtagagactt taggcaccac tacggttatc tgttcggaca aaaccgggac
     2101 tctcacggaa aacgccatga ccgtcgtgaa gatctatctt ccgtcgggca ggtttgacat
     2161 ctccggtatc ggctacgatc cttcgggaag cttcgaactt acgagcggca gaaggagcgg
     2221 tctgaacgaa cttctcacag caggtgttct ttgcaatgac gccatctaca ccaacaaaga
     2281 tggcaggcac accgtaatcg gcgatcctac agagggagcg ctgctcgttg cagctgcaaa
     2341 aggcggtctc cacaaagaag atctggaaca tctctatccc agagtgatgg aactgccatt
     2401 tgaatccagc cgcaaaaaga tgagcaccct ccacaaaacc agcagcagtt accgcctttt
     2461 tgtgaaaggc gcccccgatg aaatcctgaa aaactgcacg caagtacttt tggacggcac
     2521 tataactact ctcactcccg tgctcaggcg gactatcgct cgagaaatgg aaaccatgtc
     2581 cgatcaagcc ctgcggatcc tgggctttgc tttcaaggac tatgtcaaac tcccgaagaa
     2641 gcttacccca tcagacgaaa gcg
//
LOCUS       DAOSDA010000256         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_25005_length_2659_cov_3.001152, whole genome shotgun sequence.
ACCESSION   DAOSDA010000256 DAOSDA010000000
VERSION     DAOSDA010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25005_length_2659_cov_3.001152"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PLW82_06335"
     CDS             <1..808
                     /locus_tag="PLW82_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ46927.1"
                     /translation="IGVEFATIFSSLGTKVTIVEREKSILPQFAPQAARQAAKRLQDR
                     GVEILTNTTVTGYREQDAALIAGDVEIKGDVLLVALGNKANTVGLGLEENGVSLNQRG
                     EIVVGEDFETTKEGVYAIGDVTDKVYKLAHAASHAGVFVAEKLAGLTPHYSDELVPRT
                     VFASPEIAEVGVLEGKKSRFPFSANGRALASGEATGFVEVYGDGDRVNGVLIVGPDAC
                     ELINLAGSLLASGGSRDHLAKAILSHPSFGEALFEAGLGLYGSAVHMIKR"
     gene            complement(816..1541)
                     /gene="nadE"
                     /locus_tag="PLW82_06340"
     CDS             complement(816..1541)
                     /gene="nadE"
                     /locus_tag="PLW82_06340"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOJ46928.1"
                     /translation="MFNPEATRDLLIEWLAEYVKSAGAKGLVFGLSGGIDSAVVAGLS
                     KLAFPDTSLGVIMPCHSNPTDKEDALLVANAIGLQTQEVDLTEPFDYLVAKTGVNADT
                     MAAHNLKPRLRSSTLGLIGQSLGYLVAGGSNLAEWYTGYFTKYGDSAVDLLPLARLTK
                     SEVYQLAKVLKIPEKIITKAPSAGLWQGQTDEEEMGIKYADLDKYLTDGTGEEEIIAR
                     IEALHKRSAHKRQMPPMGPVVRG"
     gene            1661..2338
                     /locus_tag="PLW82_06345"
     CDS             1661..2338
                     /locus_tag="PLW82_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46929.1"
                     /translation="MKKNTIWDVLYQQGFAERVHRLGFGGLTASECGKIGRIFSGKGK
                     AEKGERVAVEDLTHESLAITGLKTQHMRERTRSLEHPEHHYSLTGSPVPPGTLWESRR
                     REMRRWYRLVTTDPYARLWSVELAENYVGDIDLYGIGQDIGARFFFYVASNELRTKEF
                     ITHVLGLWLGRMPFALWQVETLLSVVFVRDTVLLESLGDLGFVENGVVRLGREPACIL
                     GYKILNS"
     gene            2449..>2659
                     /locus_tag="PLW82_06350"
     CDS             2449..>2659
                     /locus_tag="PLW82_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810260.1"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
                     /protein_id="HOJ46930.1"
                     /translation="MLSDRNEMPRRAPEERAKDFKEVALGFDDDQALAEAMRCLNCKI
                     PKCVAGCPVETPIPRFIQAVKDGDLQ"
BASE COUNT          638 a          646 c          746 g          629 t
ORIGIN      
        1 catcggtgtg gagtttgcta ccatcttctc cagcttggga acaaaggtaa ccattgtgga
       61 aagagaaaaa agtattctac cgcagtttgc gccgcaggcc gcaaggcagg cagcaaaaag
      121 gctgcaggac agaggcgttg agatcctgac aaacaccact gtcaccggct acagagagca
      181 ggatgcagcc ctcattgcag gagatgtgga gatcaaaggc gacgttcttt tggtggcgct
      241 gggcaacaaa gccaacacag tcggtctcgg ccttgaggaa aacggagttt cgctgaacca
      301 aagaggagag atcgtggttg gagaagattt tgaaaccaca aaagaaggtg tttatgccat
      361 cggcgatgtc acggacaaag tctataagct tgcccatgcg gcatcccacg caggcgtgtt
      421 tgtagcggag aagctcgccg gcctgactcc ccactacagc gatgagctgg tgccgagaac
      481 tgtttttgcc agtccggaga ttgcagaagt gggcgttctg gaaggaaaaa agagccgctt
      541 tccatttagt gcaaatggaa gggctcttgc aagcggagaa gcgacaggtt ttgttgaagt
      601 ttacggtgat ggcgaccgcg taaacggtgt ccttatcgta gggcccgatg cctgcgagct
      661 gatcaaccta gcaggcagcc tccttgcctc cggagggagc agggatcatc tggccaaagc
      721 catcttgagc catccttcct tcggagaggc cctctttgaa gctggacttg gcctttacgg
      781 cagtgccgtg catatgatta aacgctaatc cgaggttagc ctctgacaac cgggcccata
      841 ggtggcatct gccgcttgtg ggcgcttctt ttatgcaggg cttcgattct cgcgatgatc
      901 tcttcctcgc ctgtaccatc ggtaaggtac ttgtcgagat cggcgtactt gatccccatc
      961 tcttcttcgt cggtttgccc ttgccagagt ccggcgcttg gggctttagt gataattttc
     1021 tccggaatct tgaggacctt tgccagctgg tagacttcgc ttttagtcag tcttgctaaa
     1081 ggcaaaaggt caactgcaga atcgccgtac ttggtgaaat agccggtgta ccattcggcg
     1141 agattgctgc ctcctgcaac gagatatccc aagctttgac cgataagccc cagagtagac
     1201 gaccgcaggc ggggctttaa gttgtgggct gccatggtat ctgcgttcac tccggttttg
     1261 gccaccaagt agtcgaatgg ttctgtcaga tctacctcct gcgtctgcag gcctatagcg
     1321 ttagccacaa ggagggcgtc ttccttgtct gtgggattgg agtggcaggg catgatcacc
     1381 ccgagggatg tatcggggaa agccagcttg ctgaggccgg ccacaaccgc agaatcgatg
     1441 ccaccgctaa gaccaaagac cagccctttt gctccagcag acttgacata ttcagctaac
     1501 cattctatga gaagatctcg agttgcttct ggattaaaca tggaaattcc acctttttag
     1561 cagtttacaa atagatgcat actaactctg tgctaccaat gaaatccttt tacattgtac
     1621 cctgatttcc aagtcaggta aaccaacgcg aggtgggatt gtgaagaaaa acacgatctg
     1681 ggacgttctc taccagcagg gttttgcaga gagagtccac aggctcggat ttggcggcct
     1741 tacagcatcc gagtgcggta aaatcggccg gatcttttcc ggaaagggca aagcggaaaa
     1801 aggcgagagg gttgcagttg aggacctaac tcatgagagc cttgccatca ccggtttgaa
     1861 aacgcagcac atgcgggaaa gaacaaggtc tctagagcat cctgaacacc actattccct
     1921 cactgggagc cccgtacctc cgggtactct ctgggagtcg aggcgtaggg agatgcgccg
     1981 ttggtaccgg ctggtgacaa cggaccccta tgccagactc tggtctgtgg aacttgcaga
     2041 aaactacgta ggcgacatcg acctctacgg catcgggcag gatatcggtg cccgcttctt
     2101 cttttatgtg gcctcaaacg agctgaggac taaagagttc atcacccacg ttcttggcct
     2161 ttggctgggt agaatgccct ttgccctatg gcaggtggaa acgcttcttt ccgttgtttt
     2221 tgtccgcgac acagtgcttc ttgagtctct tggggatctg gggtttgtgg aaaacggtgt
     2281 ggtgaggctc ggcagggaac ctgcgtgtat ccttggatac aagatactta attcatgacg
     2341 aacttacgca ttttgtggag gaaataggcc gccatgtgga aaataccttc ctaattcgca
     2401 ctagttggtg tattataagg agaaatagtt aaagaggtgg aaccattggt gctttcagat
     2461 aggaacgaaa tgccgagaag ggcacctgag gaaagagcga aggacttcaa agaggtggcc
     2521 ctcggttttg acgacgatca agcgctggct gaagccatgc gctgtctaaa ctgcaaaata
     2581 cctaagtgcg tcgcgggctg tccggtggaa acaccgatcc cccgttttat acaggctgta
     2641 aaagacggcg atctgcaaa
//
LOCUS       DAOSDA010000257         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_25028_length_2657_cov_3.230208, whole genome shotgun sequence.
ACCESSION   DAOSDA010000257 DAOSDA010000000
VERSION     DAOSDA010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25028_length_2657_cov_3.230208"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(350..940)
                     /locus_tag="PLW82_06355"
     CDS             complement(350..940)
                     /locus_tag="PLW82_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ46931.1"
                     /translation="MNIEIRKLAPEHAEDYVRFFDTTPHDDCVDEHKCYCVCWCSDDC
                     EGRDYSTVQKRREYALRYVKDGSIQGYLAYYDGKIVGWCNANTKSGCLKCVSWRRMMG
                     YVPLDDVSAGLKVKSIFCFVIAPEMRRKGIATLLLERVCQDAAEEGFDLVEAYPYKSS
                     SWQSSDFGGYLEMYQKKGFSVCAETDQGVVVRKSLR"
     gene            complement(1161..1775)
                     /locus_tag="PLW82_06360"
     CDS             complement(1161..1775)
                     /locus_tag="PLW82_06360"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOJ46932.1"
                     /translation="MRDAGGWISMTVVAEGTKTKIRSLRLEDAPLFARWWNDGRVMAA
                     VGFRKGLGVTEEKLYEDFKKEIDDENPYRERRRYVILDRETEAPRGELSYGELDTEVG
                     KCRVGIKICEISAQGRGFGKDALLAFMRYLFDRFGLQKIEIDTLKDNVRAEALYRKVG
                     FYEVKTEQNFWQDPDGIWHDLVFMEISRSEFFSAHSTSLLEAQS"
     gene            complement(1803..2306)
                     /locus_tag="PLW82_06365"
     CDS             complement(1803..2306)
                     /locus_tag="PLW82_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ46933.1"
                     /translation="MKKLIMINGTMGVGKTSVSTEILKMLTPGVYLDGDWCWYMHPFE
                     VTEETKKMVLDNITYLLRNFLSCSQYSYVIFSWVMHEESILDWIVDGLTGLEFELHKF
                     SLVCTESALTERLQKDIASGRREGDIIERSVARLPLYRQMNTIKIDVSSISAVEAAEM
                     IKEAVEK"
     gene            complement(2412..>2657)
                     /locus_tag="PLW82_06370"
     CDS             complement(2412..>2657)
                     /locus_tag="PLW82_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46934.1"
                     /translation="EYYLGTYGGLKYFAHRYFAKMNLMELARLYENVYLLWQQAFDLK
                     QKEDLNNVKVRSKIAELLEDAYENERKALEVISGLTS"
BASE COUNT          609 a          751 c          554 g          743 t
ORIGIN      
        1 cattcatgcc aaatctagta gttttaaata gcctctagct gaaagttacc acgttgtcag
       61 aacagctgaa ggttcctttg tgcttttctg tacctgaaag gagatttgac agatcgcctc
      121 ctgcgcggac tgcttccact agcgggaatg ctttgaacat ggatttcgaa cagaatccac
      181 ccggacaatc gtactcgcag taaaagacgt caccgacttc atgtctgttg cgacagcgtc
      241 taggtctccg atcatctact agtgaaagca ccttgatccg aaaactgtac tgctctttct
      301 atcatttctt cacctgtcgt tctctctact cgaccagtct atcaacgaac tacctcagcg
      361 actttctcac tacaactcct tgatctgttt ctgcacagac gctaaagcct tttttctggt
      421 acatctcaag gtaaccgcca aaatccgatg actgccagct ggagcttttg taaggatagg
      481 cttccacaag atcaaatcct tcctcagctg catcttgaca tacccgttcc agaagaagcg
      541 ttgcaatacc ctttcttctc atttccggag cgatcacgaa gcagaagatg gatttaacct
      601 tcagccctgc agatacatcg tccaaaggga cgtaacccat catcctgcgc cagctgacgc
      661 actttaggca gcctgatttt gtgttggcgt tgcaccaacc gacgattttc ccgtcgtagt
      721 atgcgaggta accttggatg ctcccgtctt ttacatagcg caaagcatac tctcttctct
      781 tctgaactgt ggagtagtcc ctgccctcgc aatcgtcgct gcaccagcat acgcagtagc
      841 atttgtgttc atcaacacag tcgtcgtgag gcgtcgtgtc gaagaaccta acgtagtctt
      901 ctgcatgttc tggcgcaagt ttccgtatct cgatgttcat acttcagccg tctcctttcg
      961 acttactctc ttgcgaacat atgtcctatt ttcgtctcct aaatcctttt ttccacaagg
     1021 tgcaccacaa tattttttgg cttttcatca gtagctctac ttaagccact cgacgcaaac
     1081 gaacaaactg cggtttcccg gttagtaccg aaccgcagca caggcaaaca agcgagtttg
     1141 ttacaaagta gcgtaaaaaa ctagctctgc gcctccagaa gcgacgtgct gtgggctgag
     1201 aaaaactccg atctcgagat ctccatgaaa acgagatcgt gccagatgcc gtccggatcc
     1261 tgccagaagt tctgttcggt cttcacctcg taaaacccaa ccttccggta taaggcctca
     1321 gctctgacgt tgtccttcaa ggtgtcgatc tctatttttt ggaggccaaa gcggtcaaag
     1381 aggtagcgca taaacgcaag cagcgcgtcc ttcccaaacc ctctgccctg agcagaaatc
     1441 tcgcagatct tgatgcccac gcggcatttt ccgacttccg tatccagttc gccgtaagag
     1501 agctcgcctc tgggcgcttc agtttctcgg tccaggatga catacctcct ccgctcgcgg
     1561 taggggtttt catcgtcaat ttccttttta aagtcttcgt aaagcttttc ctcggtcact
     1621 ccaagcccct tcctgaaacc tacagccgcc atgacccgcc cgtcattcca ccacctagca
     1681 aacagcggag cgtcttccaa acgtaaactc cggatcttgg ttttcgtgcc ctcggcaaca
     1741 accgtcatgg aaatccaacc tcctgcatct ctcatacttg atttgctgtt catcttgcta
     1801 catcacttct ctactgcttc tttgatcatc tcggccgctt cgaccgcgga gatggagcta
     1861 acatcgatct taatcgtgtt catctgcctg tacaggggga gccgggcaac gctgcgttcg
     1921 atgatatccc cttcacgcct tccagaggca atgtccttct gcagtctttc tgtcagcgcg
     1981 ctttccgtac agactaggga gaacttgtgg agttcaaact ccaggcctgt aaggccatcg
     2041 acaatccagt ccaagatgct ttcctcgtgc atcacccaag aaaaaatcac gtagctgtac
     2101 tgggaacagc tgaggaaatt cctgaggaga taggtgatgt tgtcaagtac cattttcttg
     2161 gtctcctccg tgacctcgaa gggatgcatg taccagcacc agtctccgtc caagtagacc
     2221 ccgggagtga gcatctttag aatctctgta gatacagagg tctttcccac tcccatggtg
     2281 ccgtttatca taatgagctt tttcatggca tttactccta tcttttgggc ttgtacctgc
     2341 atcttcaaaa tcatcagaac accgaatagc ttgactcccc taccgcatca ctgcttcggc
     2401 gctgtgtgga actagctggt aagtcctgat attacctcaa gcgcctttct ctcgttttcg
     2461 tacgcgtctt ccaaaagctc ggcgattttg ctccgtactt taacgttgtt tagatcttct
     2521 ttttgcttga gatcgaatgc ctgctgccag aggaggtaaa cgttttcgta gagccttgct
     2581 agctccatca gattcatttt agcaaagtac ctgtgggcga agtacttcaa ccctccgtag
     2641 gtgcctaaat agtactc
//
LOCUS       DAOSDA010000258         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_25329_length_2635_cov_2.781783, whole genome shotgun sequence.
ACCESSION   DAOSDA010000258 DAOSDA010000000
VERSION     DAOSDA010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25329_length_2635_cov_2.781783"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PLW82_06375"
     CDS             <1..697
                     /locus_tag="PLW82_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HOJ46935.1"
                     /translation="VDYAVSAGFENVSIDVIYAYPGTTMQSLEKTLQIASGLPVTHIS
                     YYGLQLEEGTELFRVQPEMLSDSEYVEMYFRGIETLENAGFVQYEISNFAKPSYQCKH
                     NLIYWKNQEYLGLGVGAVGRLGRVRYRNTEVVEEYIRSMHSGAIVQSVEEELTDELML
                     QENIMLGLRLREGVGPEDLGGSSLRELFPEAVVQLEEAGFLEEIVFRGTKRFRLTRKA
                     LPVMNSVVGYFFD"
     gene            812..1843
                     /gene="hrcA"
                     /locus_tag="PLW82_06380"
     CDS             812..1843
                     /gene="hrcA"
                     /locus_tag="PLW82_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOJ46936.1"
                     /translation="MRELNERRKCILRAITDVYIDTAEPVGSTTIARKHNLGISSATI
                     RNEMASLEEEGYVYQPHTSAGRVPSDKGYRYYVDVLLEIPEVTSEEYKKINQLAEYRK
                     EAIEKTIRRTTKLLAELTEYVSVAVIPEHRNATFRHIQLVPLDVHNILVILVVDPGFI
                     ENRVLSSPRAFSPEQLDEISAYLNQQLRGKRGEALTKTLLSELNNIVPEARGLISSIT
                     DIIQSVLTDAKDDVVVVDGLLNILDQPEFQDLDRTKLVLSVLENRKVLSKVLSSLSRN
                     SGLTVQIGEENESPELKDFSLVTATYYHCGHNLGTIGLLGPTRMTYGRAMSLVKEVAM
                     YLSELFDLL"
     gene            1866..2411
                     /gene="grpE"
                     /locus_tag="PLW82_06385"
     CDS             1866..2411
                     /gene="grpE"
                     /locus_tag="PLW82_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOJ46937.1"
                     /translation="MDEEIKATNDVNEEAKDLSEPCTDSPAEPICDAATDESEKEYSE
                     EIAQLKTKLEEYENRYLRLQADFTNFRRRVQKEKEDLLLYANMELVKSLLPVMDSLEN
                     AKEQGDTGTVQILKQLTDILYKEGLEEVNAVDQPFDPEYHFAIEQVECDKESGVIVEE
                     LRKGYTFKGALIRPSMVRVAK"
     gene            2424..>2635
                     /locus_tag="PLW82_06390"
     CDS             2424..>2635
                     /locus_tag="PLW82_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOJ46938.1"
                     /translation="MGKIIGIDLGTTNSVVAVIEGGEPTVINNTEGNRTTPSIVAFKE
                     DGELLVGDLAKRQAVMNPERTIGSIK"
BASE COUNT          762 a          576 c          686 g          611 t
ORIGIN      
        1 ggtagactat gccgtttctg cagggtttga gaatgtgagc atcgatgtga tctacgcgta
       61 tccaggaacg accatgcaga gtctggagaa aaccctgcag atcgcttccg gtcttcccgt
      121 cacccacatc tcctactacg gcctgcagct agaggaagga acggagctct tcagagttca
      181 gccggagatg ctttcagaca gtgaatacgt tgagatgtac ttccggggta tagagacctt
      241 ggaaaatgcc ggttttgtgc agtacgagat ctcgaacttc gccaagccca gctatcagtg
      301 caagcacaac ctcatctact ggaaaaacca ggagtatttg ggtcttgggg taggtgctgt
      361 cgggcgactc ggcagggtcc gctacagaaa caccgaggtg gtagaggagt acattaggag
      421 tatgcactcc ggtgctattg tgcagtcggt tgaagaagag cttacagacg agctgatgct
      481 ccaggagaac attatgctgg ggctcaggct tcgagaaggt gtggggccgg aggatctcgg
      541 aggatctagc ctgcgggagc tcttccccga agcagtagtc cagttggagg aagcaggatt
      601 tctggaagaa attgtttttc ggggcacgaa acgcttccgc ttaaccagaa aagcactgcc
      661 ggtaatgaac tcagttgtag ggtatttttt cgactaaaag actctttctg tatctgttga
      721 cattgactct ccctagtggt atctttattt atgaaagtct tagcactcgt ctttgacgag
      781 tgctaaaaat taagggtgag gtgatggcac agtgagagaa ctcaatgaac gcagaaagtg
      841 catactcaga gctatcaccg atgtctatat tgatacggcc gaaccagtag gctcaacaac
      901 tatagcaaga aagcacaatc tgggtatctc ttcggccacc atcagaaacg agatggcatc
      961 tctagaggaa gaggggtatg tctaccagcc tcatacctca gcaggaagag ttccatcaga
     1021 taaaggttat cgttattatg tggatgtgct tttggagatt cccgaagtaa cttcagaaga
     1081 gtataagaag atcaaccaat tagcggaata ccgtaaggaa gctatcgaaa agaccattcg
     1141 cagaaccaca aagcttcttg ctgaactaac cgagtatgtt tctgtagctg taattccgga
     1201 acaccgcaac gcaaccttca gacacattca gttggtacct ttagatgtcc acaatatttt
     1261 ggttattcta gttgttgatc ccggctttat cgaaaaccgg gtgctgtcga gccccagggc
     1321 gttttctccc gaacagctag atgagatctc tgcttacctc aaccagcagc tgaggggaaa
     1381 acgaggtgaa gctttaacca agacgcttct tagcgagcta aacaacatcg taccagaggc
     1441 aaggggtctt ataagcagca ttacagatat tatccaaagt gttctcacag acgccaaaga
     1501 cgatgtggta gttgtggatg gactgttaaa catacttgat cagccggagt tccaagacct
     1561 cgatagaacc aaacttgtgc tgagcgtact cgaaaaccgt aaagtcctgt ctaaggtgct
     1621 gagttctctg tcgcgaaact ctggcctgac tgtccagatc ggcgaggaga acgaaagccc
     1681 tgagcttaag gacttcagct tagtaactgc tacctactac cactgcgggc acaatcttgg
     1741 cactatcggc cttttaggtc cgactcgcat gacctacggc agagccatga gcctggtcaa
     1801 agaagtggcc atgtacttga gcgagctatt cgatctgctc taacacaata aaggaggttt
     1861 tgctggtgga tgaagagatc aaagcaacaa atgacgtaaa tgaagaagcc aaagacctct
     1921 cagagccatg cacagacagc ccggctgaac ccatttgtga tgcagctact gacgagtctg
     1981 aaaaggaata cagtgaggag attgcacagt tgaaaacgaa acttgaagaa tacgaaaacc
     2041 gctacttacg gctacaggcg gactttacca atttcaggcg gagagtccag aaagaaaaag
     2101 aggatctcct gctgtatgcc aatatggaac tggttaagtc tctgcttccg gtgatggata
     2161 gcttggaaaa cgccaaagag cagggagaca ccggtacagt tcagattctc aaacagctta
     2221 cggacattct ctataaagaa ggcctggaag aagtaaacgc ggtggatcag cccttcgatc
     2281 ccgagtatca ctttgctatc gaacaggtgg agtgcgataa agaaagcgga gtaattgttg
     2341 aggaactgcg gaagggatat acttttaaag gcgcattgat ccgcccttct atggtaaggg
     2401 ttgcaaagta ggaggtagag aagatgggaa agatcattgg aatcgactta ggaactacaa
     2461 actcagttgt tgctgtcata gaaggtggag agccaacagt tatcaataac acggaaggca
     2521 accgcacaac accttctatc gtagcgttta aagaagacgg cgagcttctc gtcggcgatc
     2581 ttgcaaaaag acaggcggta atgaacccgg aacgaaccat tggttccatt aagag
//
LOCUS       DAOSDA010000259         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_25644_length_2613_cov_2.693120, whole genome shotgun sequence.
ACCESSION   DAOSDA010000259 DAOSDA010000000
VERSION     DAOSDA010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25644_length_2613_cov_2.693120"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2041)
                     /locus_tag="PLW82_06395"
     CDS             complement(<1..2041)
                     /locus_tag="PLW82_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOJ46939.1"
                     /translation="MEEKTLKLLEFDKVKAELAKHATSSLGKELVESLVPFDNFMRVK
                     HELRETKEAYDMLNLGKEPPFGGLSDVRDEIVRASKGAVLDGAELLEISATLRLALRM
                     KQYLESSSFPWLLQLGHRLDPLSHLYRAIEETFDEDGKVADSASPELSRIRKELTIAS
                     KRLEEKLSNMIRSSEMQKYLQESLITRRGDRLVIPVKKEYRAQVPGIIHDQSASGATL
                     FIEPASVVELNNGIRLLKEQEGEEVRRILRELSFQVGQEKEKLLLNIETLARLDFAFA
                     KARYAYALKATEPEISTEGFFDVDQARHPLLKGDVVPIDYNLGKDFRTLIITGPNTGG
                     KTVTLKTIGLMILMAQAGLFIPAAEGAKLLFRKAVYADIGDEQSIEQSLSTFSSHLSN
                     IIPILDKADEDSLVLLDELGAGTDPQEGAALATSILDYLHKNGALTVSTTHYSDLKSY
                     AYFTEGVENASVEFDPETLSPTYKLIIGIPGRSNALSIARRLGLSEEIIEFAKSKLST
                     ESLEAGSLIEKLEENRRLSEKHKEETRKLREEARELKEKLEREKLRLEKEREELKQKY
                     SMLALEELEKNKKLVEELISTIREKNSLEEARDLRKTIEEKEKQLKDEVQKSEKRRQK
                     LKPTDQFKAGDQVILKQSNLPATILSIEGAKATVQAGIMKVTVPLVDLEMMPQKDS"
     gene            complement(2055..>2613)
                     /locus_tag="PLW82_06400"
     CDS             complement(2055..>2613)
                     /locus_tag="PLW82_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOJ46940.1"
                     /translation="RVVLDWGLNITNFLAFEFYKRYLSGLEIERCTLSLELKNTELRE
                     LARSVPAEVVVHGSVPVMVSENCIIGTALGTGVCRACRGFCREEGKQYYLEDRLGYRF
                     PLVSDRAGRSHIFNSKDMCLIDEVETIRHYAAQRLMFLLPDEPVDRVIRAYRKALSGI
                     PVDKADLESLSTRGFTYGHYFRGVE"
BASE COUNT          528 a          719 c          622 g          744 t
ORIGIN      
        1 cggagtcttt ctgcggcatc atctccagat ccaccaaagg tacggtgacc ttcatgattc
       61 ccgcttgcac cgtagctttc gccccttcga tgctaaggat ggttgcaggc aaattggact
      121 gtttgaggat cacctgatcc cccgctttga actggtctgt cggcttcagc ttctgacgcc
      181 tcttctcgga tttttgtact tcatccttga gctgtttttc cttctcctcg atggttttcc
      241 tcaggtctct agcttcctct agggagttct tttctctgat agtgctgatg agctcttcta
      301 ccagcttttt gtttttctcc aactcctcta gggccagcat gctgtacttc tgcttcagtt
      361 cttctcgttc tttttccagc cgcagctttt ctctctccag tttttccttg agttctctgg
      421 cttcttccct cagctttctc gtttcttcct tgtgcttttc cgacagccgc cggttttctt
      481 ctagcttttc gatcaggctg ccggcttcaa ggctttctgt tgataactta gatttggcaa
      541 actcgatgat ctcctcagaa agccctaacc gtctggcaat gctcagggca ttgctccttc
      601 cgggaatgcc gatgatcagc ttgtatgtgg ggcttagagt ctcgggatcg aactcaaccg
      661 aagcgttttc cacgccttcg gtgaagtagg cataggattt caggtccgag tagtgggtcg
      721 tggacacggt gagagcaccg tttttgtgca gatagtccag aatcgaggtt gccaaagcgg
      781 caccttcctg aggatcggta ccggccccta gttcatctag aagcaccagg gaatcctcat
      841 cggctttgtc caagatgggg atgatgttgg aaaggtgcga tgaaaaagtg ctcaggctct
      901 gctcgatact ctgctcatcg ccgatgtcgg cgtaaaccgc ttttctaaaa agcaatttag
      961 ctccttctgc agcagggata aacagcccgg cttgggccat gaggatcatc agcccaatgg
     1021 ttttaagggt aactgtttta ccacctgtat tgggacctgt gatgatcagg gtgcggaagt
     1081 ccttgcccag gttgtagtcg ataggaacaa catctccctt gagcagggga tgcctggcct
     1141 gatcaacatc aaagaagcct tcagtggaga tttccggttc tgtggccttt aaagcgtagg
     1201 cgtacctggc tttcgcaaaa gcgaagtcca gccgtgcgag ggtctcgatg ttcaagagaa
     1261 gcttttcctt ctcttgtcct acctggaagg agagctctcg caagatcctg cgtacttcct
     1321 ctccctcctg ctccttcagg agccggatgc cgttattcag ctccaccacc gatgcaggtt
     1381 cgataaacaa agtggcgccg cttgcggact ggtcgtggat gattcctgga acctgagccc
     1441 ggtactcctt tttcacggga atcacgagcc tgtcgcctct tctggtaatc agggattcct
     1501 gcaggtattt ttgcatttcc gatgagcgga tcatgttgct tagcttctct tcaagtcgct
     1561 ttgacgctat tgtcagctct ttgcgaattc tggaaagttc tggtgatgca gagtctgcta
     1621 ccttgccgtc ctcgtcaaag gtctcctcga tcgcccggta gaggtgggaa agaggatcta
     1681 ggcggtgccc aagctgcaga agccagggga aactgctgct ttcaaggtac tgcttcatcc
     1741 gcaaggccaa cctcaatgta gccgagattt cgagtagttc tgcaccatca agcactgctc
     1801 ctttagatgc cctcacgatc tcatcccgca catcgctgag gccgccaaaa ggtggttctt
     1861 tcccgaggtt cagcatgtcg taagcctctt ttgtctcccg cagttcgtgc ttcactctca
     1921 taaaattgtc gaagggtaca aggctttcca ccagttcctt gcctagagag ctggttgcgt
     1981 gttttgccag ttctgctttt accttgtcaa actctaacaa cttcagcgtc ttttcttcca
     2041 actcaaaccc tcctctattc cacacctctg aagtagtggc cgtatgtgaa tcctctggta
     2101 gacaggctct ccagatctgc tttgtcaaca ggtatgccgc tcaaagcctt cctgtatgcc
     2161 ctaataaccc gatctactgg ctcatcaggg agaagaaaca tcaaccgttg cgctgcgtag
     2221 tggcggatag tttctacctc gtcgattaaa cacatatcct tggagttaaa aatatggctt
     2281 cttcctgccc ggtccgatac aagagggaac ctgtagccaa gcctgtcctc aaggtagtac
     2341 tgcttcccct cttcccggca aaatcctctg catgccctgc agactccggt gcccagggca
     2401 gttccgataa tgcagttttc acttaccata accggtacgc tgccgtgaac aaccacctct
     2461 gcaggcaccg atcgggccag ctccctcagt tcagtgtttt tcagctccag agacagggtg
     2521 cacctttcga tctcaagacc tgacaggtag cgcttgtaga attcaaaagc gagaaagttg
     2581 gtgatgttca atccccaatc caaaaccacg cgg
//
LOCUS       DAOSDA010000260         2603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_25786_length_2603_cov_2.598509, whole genome shotgun sequence.
ACCESSION   DAOSDA010000260 DAOSDA010000000
VERSION     DAOSDA010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25786_length_2603_cov_2.598509"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PLW82_06405"
     CDS             complement(<1..725)
                     /locus_tag="PLW82_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46941.1"
                     /translation="MGLIVLVLLLSIISQPALGQSAQIALFWQDQPVESIGPQIKQGT
                     IYIPIKVMEYAGAAVHIGQSDVRVDFRGQTFEFFPGETDSNGSFAEFEGEYYLSSAEL
                     KRLFNLDIQWNSSERKIVVKEPEIKRQIIGLDFDDSQGRAVLKILHQGKAEYQVTKLD
                     PGRFKIDLFRTTLAKPLGMLLMDHPLVISVRAEQLSRDQVTFYIQGREGLAVESRLDQ
                     DNLLLEFFYNVESFEYRKMGGLP"
     gene            864..2102
                     /locus_tag="PLW82_06410"
     CDS             864..2102
                     /locus_tag="PLW82_06410"
                     /inference="COORDINATES: protein motif:HMM:NF025841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HOJ46942.1"
                     /translation="MCSVFSWQKRHPYPTADGSFIQPWLASGWDRKAWLKELEVLKEA
                     DMTYLILAPSVRDRNGKLSQAIYPSNLPWIEKQQHDTIEACLSAAESLGIKVFLGLNE
                     DAKWWKMTENDREWYIGEMHRGNEIAAEIYSHYKNRYPETLHGWYFVWELSSQHLYKT
                     EYQIFFAQAMNLQLDFLTQLDRSMPLMFCPYMTDTIHSPEETYRAWSTFFEHSRLRPG
                     DIFCPQDAVGAGWLKLENFTDYFIACKKAAEKVEGLLMWSDVETFCQETWTSTTLDRF
                     IRQMELVSGIVDNYITFAYSHYFSPNIVPKGYHRAYLEYLKHGKIFKREVPAPDVSWK
                     VQDGNLHLNWEKQSLETVCGYLIYQDGKLLAKLQLKPDRNLQFELPNSFIVLNAEGMK
                     TSIVAYDYKGNLAEPVDIIV"
     gene            complement(2163..>2603)
                     /locus_tag="PLW82_06415"
     CDS             complement(2163..>2603)
                     /locus_tag="PLW82_06415"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HOJ46943.1"
                     /translation="IEEQYGPTNLSGIATDDGFLIRGSANPSLIYNAAVIGLTRIQRG
                     EKDLALHKRCGTSIAITNLFTSIVFILFLLITGRANIWTVFLAIALGSLIGPTLGVVT
                     QKYITTRTDLSDVEIVGYVPGFGQIKVLTRDKASRDREPVIYIE"
BASE COUNT          681 a          672 c          605 g          645 t
ORIGIN      
        1 atggggagac cgcccatttt ccggtactca aagctctcta cattgtagaa gaactcgagc
       61 agtaggttgt cttgatcgag gcggctttca actgccagcc cttctctgcc ttgaatgtaa
      121 aaggtgacct ggtctctgct gagctgctct gcccttaccg agatcaccag agggtgatcc
      181 atcagcagca tgccaagggg ttttgccagc gtcgtgcgga aaagatctat tttgaagcgt
      241 ccgggatcga gttttgtcac ttggtactcg gccttcccct gatggaggat tttaagcact
      301 gctctcccct gggagtcatc gaaatccaga ccgatgatct gacgcttaat ctccggttct
      361 ttgacaacga tcttcctttc gctgctgttc cactggatgt ccaaattgaa aagccttttg
      421 agttccgccg aactcaaata gtactcccct tcaaactctg caaaagagcc gtttgagtct
      481 gtttcgcccg gaaaaaactc gaaagtttgc ccgcgaaagt cgactctcac atccgactga
      541 cctatatgaa cagctgctcc tgcatactcc ataaccttaa tagggatata aattgttccc
      601 tgtttgatct ggggaccgat gctttccaca ggctgatcct gccaaaacag ggcaatctgt
      661 gcgctctggc ctagagctgg ttgggaaatg atggaaagta gcaaaacgag tacgattaag
      721 cccattgaca gtcccctttc gtcctttctt ccactttatt gtacttttaa tgaacggtaa
      781 tagcaaattg ttcctatagc aaaggaagcc ggtatcctcg gctcgaacat agaaccagcg
      841 ataacaagga gggtgaagat agaatgtgca gcgttttttc gtggcagaaa aggcacccgt
      901 atcctacagc ggacggttcc ttcatccaac cgtggctcgc aagcggttgg gacaggaaag
      961 cttggttgaa agaacttgaa gtgctgaaag aggcggacat gacctacctc attcttgctc
     1021 caagcgttag agaccgcaat ggcaagcttt cacaagcgat ctatccctca aacctcccgt
     1081 ggatcgaaaa gcagcagcac gacaccatag aagcttgtct ctctgccgcc gaaagcctcg
     1141 gcatcaaggt tttcctggga ctgaacgaag atgccaaatg gtggaaaatg acagaaaacg
     1201 accgtgagtg gtatataggc gagatgcaca gaggtaatga gatcgcagcc gaaatttact
     1261 cccactacaa gaaccgctat cccgaaaccc ttcacggctg gtactttgtt tgggagctat
     1321 ccagccagca cctctacaaa accgaatacc agatcttctt tgcacaggcc atgaatctac
     1381 agctggactt cttgacacag ctcgaccgat ccatgcccct gatgttttgc ccctacatga
     1441 cagacaccat ccactcgcca gaggaaacct accgggcttg gagtacgttt tttgagcatt
     1501 cacgactgag gcccggagac attttctgcc ctcaggacgc tgttggcgct ggatggctga
     1561 agctggaaaa ctttaccgac tactttatcg cctgcaaaaa ggctgcagaa aaggtcgaag
     1621 gactgctgat gtggtccgat gtggagacct tctgtcagga aacctggacc agtaccactt
     1681 tggacaggtt catcaggcag atggaactgg tctctggtat tgtcgataac tacatcacct
     1741 ttgcctattc acattacttt agtcctaaca tcgtccctaa aggataccac cgagcttacc
     1801 tcgagtactt aaaacatggg aaaatcttca aacgcgaagt gcccgcccca gatgtgtcgt
     1861 ggaaggtgca ggacggaaac ctccatctca actgggagaa acagagcctt gaaacggttt
     1921 gcggctattt gatctatcag gatggcaaac tccttgccaa actgcagcta aagccagaca
     1981 gaaaccttca gttcgaactg cccaatagct ttattgtttt gaatgcggaa ggcatgaaga
     2041 cctccatcgt agcctacgac tacaagggaa accttgcaga acctgtagat atcatcgttt
     2101 agcctgaagc aaacctgctc cctaaaagaa agcgattctt ctttcaggga gcaagttgtc
     2161 ttctactcaa tatagataac tggttctctg tctcgagacg ccttatctct ggtcaggacc
     2221 ttgatctggc cgaacccggg cacgtatcct acaatctcga cgtcgcttag atcggttctg
     2281 gtggtgatgt acttctgcgt caccacgcct agagtaggcc cgattagact ccccagggcg
     2341 atcgcaagaa acactgtcca aatattagcc ctacctgtga tcagtaagaa taagataaag
     2401 actattgacg taaagagatt ggtaatggcg atacttgttc cacacctctt atgcagagct
     2461 aaatcctttt ctcccctctg gattctggta aggccaataa ctgcagcgtt gtagatgaga
     2521 gagggatttg cagagcctct aatcaagaac ccatcatcag tagctatccc gctcaagttc
     2581 gttggcccgt actgctcctc gat
//
LOCUS       DAOSDA010000261         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_25895_length_2595_cov_3.061024, whole genome shotgun sequence.
ACCESSION   DAOSDA010000261 DAOSDA010000000
VERSION     DAOSDA010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25895_length_2595_cov_3.061024"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /gene="metG"
                     /locus_tag="PLW82_06420"
     CDS             complement(<1..1207)
                     /gene="metG"
                     /locus_tag="PLW82_06420"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019543094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOJ46944.1"
                     /translation="MGKSTKNTYYITTPIYYPSDRLHIGHAYTTTIADAFARFNRLRG
                     KDVFFLTGSDEHGLKIQRIAESRGMTPKEYVDGIVRSFQALWQRLNITYDDFLRTTDE
                     RHEKLVQKIFQILYEKGEIYKSSYKGLYCTPCETFWLERQVEDGKCPDCGRPVEEVEE
                     ESYFFRLSKYGQPLLDYYKAHPGFIQPESRLREMEAFIKSGLEDLCVSRTAVSWGIPV
                     PFDPDHSIYVWFDAVINYLSALGYLSEDESKYQKYWPADLHLVGKEIMRFHTIIWPAI
                     LLALDIPLPETVFGHGWLVIEGEKMSKSKGNVVDPNLLIDEFGADAIRFFLLREITFG
                     QDGNFSRDALIRRINSDLANDLGNLLHRTSSMYAKYFPEAVPALGEEEDAEKELAQFA
                     KDAISKFEEK"
     misc_binding    complement(1266..1510)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1579..2424)
                     /gene="rsmI"
                     /locus_tag="PLW82_06425"
     CDS             complement(1579..2424)
                     /gene="rsmI"
                     /locus_tag="PLW82_06425"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOJ46945.1"
                     /translation="MKYPEINESLESRTLYVVATPLGNLGDITLRALYVLGNVHAILC
                     EDTRRTATLCRYYDINTPLMSFHSHSPESRFAEIKEILQTEALALVSDAGTPCISDPG
                     AKLVAELVDEGFNVRPVPGPSALTAALSASGLDTREFTFRGFLGKKGLKNKVKTIMDR
                     EELQVVYESPNRLAELLEIIKDIDSKRQLVVTRELTKVFEEFYRGTAEECHTYFTSKE
                     VKGEITLIISGKSSGDYSEEDLAKLIQEALDSGETLSKAAKRLAKELGVPKNAVYEIG
                     LKLTE"
BASE COUNT          627 a          657 c          567 g          744 t
ORIGIN      
        1 ttttttcttc gaactttgaa atggcgtctt tggcaaactg agctagttcc ttttctgcat
       61 cttcttcttc tcctagagca ggcacagctt ccgggaagta tttggcgtac atagagctgg
      121 tcctgtgaag gaggttgcca aggtcgttgg caagatctga attgattctt ctgatcagcg
      181 cgtctctaga gaagttgccg tcctgaccaa aagtgatctc ccgaagcagg aagaagcgta
      241 tggcatctgc cccaaactcg tcgatgagaa gattgggatc gaccacattg cccttggatt
      301 ttgacatttt ctcgccttca ataaccagcc acccgtgtcc gaagactgtc tcaggcagag
      361 gaatgtccag agcaagcaat attgcgggcc aaataatcgt atggaacctc attatctcct
      421 tgcccacaag atgcagatct gccggccagt atttttggta cttggattcg tcttcagaaa
      481 gatagcccag agcagagaga tagttaatca cagcatcgaa ccacacgtaa attgagtgat
      541 ccggatcgaa gggaacagga ataccccaac ttacagcagt tctggaaaca cataggtctt
      601 ccagaccgct ttttatgaaa gcttccatct cgcgcaatct ggactcgggc tggataaagc
      661 cgggatgggc tttgtagtag tccaaaagcg gctgtccgta ttttgaaagg cggaagaagt
      721 agctctcttc ttctacctct tcaactggtc ttccgcaatc agggcacttg ccgtcttcaa
      781 cttgcctttc cagccaaaaa gtctcacacg gagtgcagta taaccccttg tagctactct
      841 tgtagatttc gcctttttcg taaagaattt ggaagatctt ttgcacgagc ttctcgtggc
      901 gctcgtctgt tgtccgcaga aaatcgtcat aggtaatgtt cagccgctgc caaagggcct
      961 ggaagcttct gactatacca tcaacgtact ctttgggagt catgcctctg ctttcagcaa
     1021 tacgctgaat cttgagaccg tgttcgtcag agccagtaag gaaaaacaca tctttgcctc
     1081 tcaagcggtt aaatcttgca aatgcgtcag ctatggttgt tgtgtacgca tggccgatgt
     1141 gcaacctgtc gctggggtaa tatattgggg ttgtaatgta ataagtgttc ttagtagact
     1201 ttcccatagt ttctacctcc ttcagtttga ttaccgataa atttggtaaa aaataaagct
     1261 cgccccgaag ggacgagcat agaacccgcg ttaccaccct aattcttacc agaacacttc
     1321 tctggtaagc tctccttttc aggtaccatc ataccctagc gctgtaacgg gcacacccga
     1381 agcaacctac tccactttca attgctcagc tccgaagcca tgttcagcag ttaagtcttt
     1441 gctggctttc acctaaccca gctctctaag aaaaaccacc ctgcttactc ttcttcttca
     1501 ctgctttttt agtcttttct gtctttacaa gaagatacca cttgatgaga gattcgtcaa
     1561 gtagctggtt aaaacccact actcggtcag cttcaatcct atttcatata ccgcgttttt
     1621 cggaactcct agctctttgg caagtctttt tgcagctttg gacaaagtct ctccgctgtc
     1681 tagtgcctct tgtattagtt tggctaaatc ttcttcgctg tagtccccag aactcttccc
     1741 tgagataatc agagtgattt cccccttaac ctctttggag gtgaagtagg tatgacactc
     1801 ctcggcagtg ccgcggtaaa actcctcaaa aactttggta agttctctag ttaccacgag
     1861 ctgtctttta gaatcaatgt ccttgatgat ctccaaaagc tccgcgagcc tgtttggcga
     1921 ctcgtagact acctgcaatt cttccctgtc cataatagtc tttactttgt tctttagacc
     1981 tttctttcct aaaaaaccgc gaaaggtgaa ctctcgcgta tcgagccctg aagcagaaag
     2041 tgctgccgtg agcgcagatg gccccggaac aggtctaacg ttaaagccct catcaaccag
     2101 ttcggcaacg agctttgcgc ccggatcgga gatgcagggc gtcccggcat cgctgaccaa
     2161 agctagggct tcagtctgga ggatctcttt aatctcggcg aatctacttt caggagaatg
     2221 gctgtggaag ctcattaagg gagtattgat atcatagtac ctacacagcg tcgctgtccg
     2281 ccttgtgtct tcacacaaaa tcgcgtgaac attgcctaag acgtaaagtg ctcttagggt
     2341 tatgtcaccc aggttcccta gtggtgttgc caccacgtac aaggttcggg attcaaggga
     2401 ttcgttgatc tcggggtact tcaaagcaac cacctaacct tacctagagc acttgcttac
     2461 ttaaaacagt ttttgccgtt tggacacgac ttatgatcgc aaccgccttt ggccttaaaa
     2521 ttgcgacctt taggcttggg attgtctagt tcttcgtcat cgagaatcag ttcttcatcg
     2581 tcatcatcga tgtcc
//
LOCUS       DAOSDA010000262         2579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_26099_length_2579_cov_2.928685, whole genome shotgun sequence.
ACCESSION   DAOSDA010000262 DAOSDA010000000
VERSION     DAOSDA010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26099_length_2579_cov_2.928685"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1962
                     /gene="uvrA"
                     /locus_tag="PLW82_06430"
     CDS             <1..1962
                     /gene="uvrA"
                     /locus_tag="PLW82_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOJ46946.1"
                     /translation="PDLVVKNDLSIAEGALVTLPDRPDSWNQILLDQLLETEKIDKHI
                     PFRELPKKAQDLILYGSPKEYKIAYTAKRTNITRNYLARWEGIIPQMERRYSETVSDT
                     QKSYYESFMSAIHCPKCDGNRLKPEALAVTVGGKNISEVSDMSIKACKHFFENLELTE
                     REQTIARQIIKEINARLSFLANVGLDYLTLSRAAGTLSGGEAQRIRLATQIGSGLVGV
                     LYILDEPSIGLHQRDNNKLLEALKGLRDLGNTVVVVEHDEDTIRTADYIVDIGPYAGT
                     HGGEVVAQGTLKDIMNEPRSITGQFLSGKRAIHVPKERRKPNGKKLVIRGARENNLKN
                     VDVEIPLGVFTCVTGVSGSGKSTLVNEILYKALARDLHRAMVKPGAHDRIEGIEHLKK
                     VIEIDQSPIGRTPRSNPATYTGVFDAIREVFEKTPEAQARGYKKGRFSFNVKGGRCEA
                     CSGDGIIRIEMHFLPDVYVPCEVCHGKRYNRETLQVKYKGKSIADVLDMRIDEALEFF
                     ENIPSIRRKIATLCDVGLGYMKLGQPATELSGGEAQRVKLATELSRRSNGQTLYILDE
                     PTTGLHIADVEKLLDVLQKLVEAGDSVLVIEHNLDVIKTADYIIDLGPEGGDEGGWVI
                     AKGTPEEVAEVEHSYTGQFLKRILQNESR"
     gene            1967..>2579
                     /locus_tag="PLW82_06435"
     CDS             1967..>2579
                     /locus_tag="PLW82_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOJ46947.1"
                     /translation="MFPVIISAEPDELKFFLQDFKLEREDVRGFTRMFWGEYQGRKIG
                     LACLGVGKVNAAAGTALVVEHMQPDALFMVGASGALSPDVVPGDVVVGTEMIDRDVGY
                     LTKKGHIPGGAWIYDQTKPYYPYLVRSILPDEKLLQTAQAAAKEKLALESFGEREAMV
                     FFGPIATGEQFVAALEVKEQLYQNYGALAGEMEGSSLAQVAYAL"
BASE COUNT          775 a          598 c          647 g          559 t
ORIGIN      
        1 ccagatcttg tagtgaaaaa cgatttgagc attgcagaag gagctttagt tacacttccc
       61 gacagacctg attcttggaa tcagatactc ctagatcagc ttcttgaaac cgaaaagata
      121 gataaacaca tcccgtttag agagctgcct aaaaaagcac aagatctcat tttgtacggg
      181 tctcctaagg agtacaaaat agcttatact gcaaagcgga ccaacataac cagaaactac
      241 cttgcccgct gggaaggcat cattccccag atggaaagaa gatacagcga gacagtcagc
      301 gacacccaaa aatcgtacta cgaaagcttc atgagcgcta ttcactgtcc aaagtgcgat
      361 ggcaatcgct tgaaaccaga agcacttgca gtcacagtcg ggggtaaaaa catctccgaa
      421 gtaagcgaca tgtccatcaa agcctgcaag cacttcttcg aaaacctcga acttacagaa
      481 agagagcaga caattgcccg ccagattatc aaagagatca atgcaaggct ttctttcctt
      541 gccaacgtag gcctggacta cctcactctt tccagagcag caggtactct ctcaggcggt
      601 gaagctcaga gaattagact ggcaacccaa atcggctctg gtctcgttgg ggtgctctac
      661 atcctcgacg aaccgtccat cggcctccac cagagagaca acaataagct tctagaagca
      721 ctgaagggct taagagattt gggtaacacc gttgttgtag tagagcacga tgaagacact
      781 atccgtactg cagactatat tgtagatatt ggtccctatg ccggcactca tggaggagag
      841 gtagtggctc agggaacctt aaaagatatc atgaacgaac caaggtcgat cactggccag
      901 ttcctttcgg gcaaacgagc tattcacgtg ccgaaagaaa gaaggaagcc caacggtaag
      961 aagctggtga tcagaggagc cagagaaaac aacctgaaaa acgtcgacgt agagatacct
     1021 ctcggagttt tcacctgcgt cacaggcgta tcaggttctg gcaagagtac cctggtaaac
     1081 gagatacttt acaaagccct ggcccgagat ctacacagag ctatggtgaa accaggagct
     1141 catgaccgaa ttgaaggaat agagcactta aagaaagtca tcgagataga ccaatcgcca
     1201 atcggcagaa ctcccaggtc aaaccctgca acttacacgg gggtttttga tgccattcgc
     1261 gaggtatttg aaaagacccc cgaagcccaa gccagaggct ataagaaagg ccgtttcagc
     1321 ttcaatgtca aaggcggaag atgtgaagcc tgttcaggag acggcataat tcgcattgag
     1381 atgcattttc ttcccgacgt gtatgtacct tgtgaggtct gccacggtaa acgctataac
     1441 agagagaccc tacaggtaaa gtacaaaggc aaaagcatcg cagatgtctt ggacatgaga
     1501 atagatgaag cattggagtt ctttgagaac attcccagca ttagaaggaa gatcgctact
     1561 ctctgcgatg taggccttgg ctacatgaag ctggggcagc ctgctactga actctcaggt
     1621 ggagaagctc agagagtgaa gctggccaca gaactcagca ggagatcgaa cggacaaact
     1681 ctctacatcc tagatgagcc aaccactggt ctccacattg ccgatgtaga aaaactcctg
     1741 gatgtactcc aaaagctggt agaagctggc gattcggtct tggtaatcga acacaatctg
     1801 gatgtaatca agactgcaga ctacatcatc gatctcgggc cggagggcgg cgatgaaggc
     1861 ggatgggtca ttgcaaaagg tacaccagaa gaagtagcag aagtagagca ctcctacaca
     1921 ggacagttcc tcaagcgcat cctccaaaac gaaagcaggt gagagtatgt ttccggtaat
     1981 aatctcagca gaaccagatg agctgaagtt ttttcttcaa gatttcaagt tggaaagaga
     2041 agacgtccgc ggctttactc ggatgttttg gggagagtat caaggcagaa aaatcggact
     2101 tgcctgcctc ggtgtgggga aagtaaatgc agcagcggga actgctctag tagttgagca
     2161 catgcaaccc gatgcactgt ttatggtagg tgcttctggt gctctgtcgc cggatgtggt
     2221 tcctggcgat gttgtggttg gtacggaaat gattgataga gacgtagggt accttacgaa
     2281 aaaaggtcac attccaggcg gagcatggat atatgaccaa acaaaaccct actatcccta
     2341 tcttgtccgt tccatcctgc ccgacgaaaa gcttttgcaa actgcccaag cagcagctaa
     2401 agagaaactc gctttagaat ccttcggcga aagagaagcg atggtgttct tcggccctat
     2461 tgctacaggc gagcaattcg ttgctgcttt agaggtcaaa gaacaactgt accaaaacta
     2521 cggtgctctc gcaggagaga tggaaggatc cagtcttgcc caggtggcgt atgctttga
//
LOCUS       DAOSDA010000263         2563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_26338_length_2563_cov_3.550239, whole genome shotgun sequence.
ACCESSION   DAOSDA010000263 DAOSDA010000000
VERSION     DAOSDA010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26338_length_2563_cov_3.550239"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1238
                     /locus_tag="PLW82_06440"
     CDS             126..1238
                     /locus_tag="PLW82_06440"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ46948.1"
                     /translation="MKVPSFSLTKQNEMLYDEIMEALGGLIKSGQFIMGPDLVKLEEE
                     IGSRCKSKYAYGVSSGTDALQLALLAAGIGPDDEVITTPFTFVATATSILHVGARPVF
                     CDIDPKTFNIDADQIEGLITDRTKAIIPVHLYGQMADMDKIMNVARKHNLVVIEDAAQ
                     AFGATYKSEPACSFGDLACLSFYPTKNLGAFGEAGMVLTSNEKYAQRVKLLRVHGASN
                     RYFHDMLGYNSRMDTMQAAILNVKLKYFDSWTEKRRQIAKLYDTLLGDLPEVTIPYKD
                     ENAGHIYHQYTIRVKKRDVVYRELSLMGISTMIYYPLPLHLQPIMQHLGYKKGDFPET
                     EKAANEVLSLPMFPELTEEQVKLVADELKKVLAGLR"
     gene            complement(1360..>2563)
                     /locus_tag="PLW82_06445"
     CDS             complement(1360..>2563)
                     /locus_tag="PLW82_06445"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOJ46949.1"
                     /translation="LLLFHGETNFTQLYGLNLKMDRDTCTILLHKVLSFYDLPGEIEN
                     VRQCTNGHINCTYHVNLKKPDGTKDQLIIQKINSYVFKDPAKVMENIATIEEHVKNKI
                     SRGECGIISFLRSKDGKNYIELDGEYWRICPYVPDSVAFDTVEDSEVLYSAGYAFGKF
                     QELLSDIPMDKLHITIPDFHNTRKRLDDLEKMADLDPHGRAKDVQEELAFFAKYRDLA
                     TKICDMHDKGELPLRVTHNDTKYNNILMHRETLEPLCVIDLDTVMPGFSMHDFGDAVR
                     FAANTAPEDETDLSKVSLNLEHFKQFARGYMAASKNLTKNEIDTMALGAVTMTIEVAS
                     RFLLDHINGDKYFKIHRDNHNLDRARCQIRLAEDMIAKLDEMNRIVYDSLPVNCDLQN
                     EAAPVSTM"
BASE COUNT          667 a          552 c          606 g          738 t
ORIGIN      
        1 tacgctgagt tcatctaacc gagggatttt acacacactt atggacttga tccaattatg
       61 ttataaaagt gaattctagg tgtattacct aatatataaa ttggagtgga gtttttgaca
      121 tggaaatgaa agtcccatca tttagtctga caaagcagaa cgaaatgctc tatgacgaga
      181 tcatggaggc gctcggcggc cttattaaat caggacagtt tattatgggt cctgatttag
      241 taaagctgga agaagagatt ggcagtcggt gcaagagcaa atacgcctac ggcgtatcca
      301 gtggcaccga cgctctgcag cttgcacttc tcgcggcagg gataggtccg gacgatgaag
      361 taatcactac cccttttacc tttgttgcaa cagccacaag cattcttcat gtgggtgcaa
      421 ggcccgtttt ttgcgatatc gatcctaaga cctttaacat cgatgccgat cagattgaag
      481 gtctgataac cgatagaacg aaggctatta tcccagtcca tctctatggc cagatggctg
      541 atatggataa gatcatgaat gttgcaagga aacacaactt ggtagttatt gaagacgctg
      601 cgcaggcttt cggtgcaacc tacaaaagcg agcctgcttg ttcttttggc gatcttgcct
      661 gcttgagctt ttaccccaca aaaaacctgg gtgctttcgg tgaagcgggc atggttctaa
      721 ccagcaatga gaagtatgcc cagagagtaa agcttctcag agtacatgga gcatcaaaca
      781 ggtacttcca cgatatgctt ggctacaact cccgtatgga caccatgcaa gctgctatcc
      841 taaatgtgaa gctcaagtac ttcgacagtt ggaccgaaaa gagaaggcag atagcaaagt
      901 tatacgatac tctcttggga gatttgccag aggtaaccat tccgtacaaa gatgaaaacg
      961 ctggacacat ctatcatcag tacaccatca gggtgaaaaa gcgcgatgtg gtctacagag
     1021 agttaagtct tatgggcatc tcaacgatga tctactatcc gctacctctc cacttgcagc
     1081 cgatcatgca acatttaggc tacaagaaag gtgacttccc cgaaacagaa aaagcagcca
     1141 atgaggtttt gtctctcccg atgttcccgg aacttaccga agaacaggtt aagttggttg
     1201 cagacgagct gaagaaggtg ctcgcggggc tcagataatt tcttgaggca acatatgatt
     1261 atcagaaaaa gggtctggat ctctttgcga gatccagacc ctacttattg gcgtcgaaag
     1321 aacttgtatc aacctttatt gtactcctgt gactccaagt tacatggtac ttacgggagc
     1381 tgcttcattt tgaaggtcgc aatttacggg gagactatca tagacgatgc ggttcatctc
     1441 gtcgagcttg gcgatcatgt cttcggcaag cctgatttga catctggctc tatctagatt
     1501 gtgattgtcg cggtggatct tgaagtactt gtctccgtta atgtggtcta gtaggaacct
     1561 tgatgcaacc tctatggtca tggtcaccgc acctaaagcc atggtgtcga tttcattctt
     1621 tgtaaggttt tttgatgcgg ccatgtagcc tctagcaaac tgcttgaagt gctctaggtt
     1681 taaactgact ttcgagagat cagtttcgtc ttctggagct gtatttgctg caaaacgaac
     1741 tgcgtcacca aaatcgtgca tagagaaacc cggcattaca gtatctaagt cgataacgca
     1801 caacggttca agggtctctc tgtgcataag gatgttgttg tatttggtgt cgttgtgagt
     1861 taccctaagc ggcagttctc ctttgtcgtg catgtcgcag atcttcgttg caaggtctcg
     1921 gtattttgcg aaaaaagcta actcttcctg gacgtctttt gctctgccat ggggatcgag
     1981 atctgccatt ttctcaagat cgtccagcct cttccttgtg ttgtggaaat cgggaatggt
     2041 tatgtggagc ttgtccatgg ggatatcgga aagcagttcc tggaacttgc caaacgcata
     2101 ccccgcgcta tagaggactt cgctgtcttc gacagtgtcg aaagcgacag agtccggaac
     2161 gtacgggcaa attctccagt actctccgtc tagctctatg tagttttttc catctttgct
     2221 cctgagaaaa gaaataatgc cgcattcgcc gcgacttatc ttgtttttga cgtgttcttc
     2281 gattgtggca atgttctcca tgacttttgc agggtcttta aatacatagc tgttaatctt
     2341 ttgaatgatg agttgatcct tcgtaccatc gggttttttg agatttacgt gatatgtaca
     2401 gttgatgtgg ccatttgtgc actggcgtac attttcaatc tcacctggta agtcgtagaa
     2461 actcaaaacc ttatgcagta gtatagtaca agtgtctcgg tccattttca agttgagtcc
     2521 gtataattgt gtaaaattgg tttccccatg aaataaaaga agc
//
LOCUS       DAOSDA010000264         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_26395_length_2559_cov_3.867013, whole genome shotgun sequence.
ACCESSION   DAOSDA010000264 DAOSDA010000000
VERSION     DAOSDA010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26395_length_2559_cov_3.867013"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2269
                     /locus_tag="PLW82_06450"
     CDS             <1..2269
                     /locus_tag="PLW82_06450"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOJ46950.1"
                     /translation="FTSKTTTPELWEKALFLGSNETCLPVRIYIAHCHRLVEDLKVDV
                     LIAPNLWREVEGTAACSKYRDVGGIALRALSSVADYALGKLPYLIQPDILSPKKEDLL
                     PVVYEVINELRSAAARLGEPFPKISKDQINKAFAKVPDFAQECEVELEDKPRIGIIGR
                     RYILEDPLLSLNIIRFFKKKGFQVVTPYDLPDRYFTKEGLPFGSYYDTHIQGEALLNW
                     GRDKLHGVVILSNFACHPDAFQGEYFAQKAAELGLLAWRFVCDATVDLGGFTTRFETI
                     AVLIEQNMQKTAARKGQLAVANTGHGAPVSYSAAVPSQSHVSPWQQSRETLLPTKSQS
                     PRLEIVQKPKLKPKTIVTWPYMGEPLNLALKQLVFDLGAGEFAVPPKAVSEETLKRGS
                     EDLAETCCPYALISGSLEETILGVYEEAGRPIDAKILMLHGEGPCAFGWYSIALKEKL
                     PKRLKQVSPEISLEWFTTGIDQSVSFWQSLASFCDEKFERLLSSFKSPLSFLQPKNLG
                     DVLPVVRNALTKLTLTEKMRGLYLMARPFGGPAAEAAYITSLNSLDEAKNTRDMQKSY
                     RDAREIFAAIIKDRTAPQVVIVGEIYVAITPYANRYAVDRLLGDAGLQVIEGITVSHY
                     LKCSLRPAAKRLMQNLSLFKYANKKGLKLFAQGLRDKDSAPYMNFEVGGDGVLSVASA
                     RRALHSGKDGIVQIYPLNCMPEVVAKPAISELAAIYDRPFLSLSFNRETDTERLMTEV
                     LTFARLIKNARKTSS"
     gene            complement(2250..>2559)
                     /locus_tag="PLW82_06455"
     CDS             complement(2250..>2559)
                     /locus_tag="PLW82_06455"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOJ46951.1"
                     /translation="LFGLLGFVLYARVFRYSHVTPAIQSSLLTVLAINFLIALVPGSN
                     IDVWSHVGGLLGGFLIGMIIGPSSRYLRSDSEKTTQITLGVLGIVAVLAVTFLNLRFF
                     "
BASE COUNT          671 a          706 c          632 g          550 t
ORIGIN      
        1 ctttacttca aaaaccacaa ctcccgaact ctgggaaaag gctctttttc tcggaagcaa
       61 cgaaacctgc cttcccgtta ggatctacat tgcccactgc cacaggctgg tagaagacct
      121 taaagtagat gtgctgatcg cacccaacct ctggcgtgag gtggaaggca ctgctgcttg
      181 ctccaaatac cgcgacgtag gaggtatagc gctgagggct ctatcgtcag ttgctgacta
      241 cgctctcggc aaactcccct acctcatcca accggatatc ctctcgccaa agaaagagga
      301 tcttctaccg gtagtctacg aggtcataaa cgagctacgt tctgcagcag ctcgcttagg
      361 cgagcccttt ccaaaaatct ccaaagacca gataaacaaa gcctttgcca aagtacctga
      421 cttcgcccaa gagtgcgaag tggaactaga ggacaaaccg aggattggga tcatcggcag
      481 acgctacatt ctcgaggatc cgctcctttc cttgaacatc attaggttct tcaagaaaaa
      541 gggcttccaa gttgtcactc cttacgatct cccggatcgg tatttcacta aagaagggct
      601 gccctttggc tcctactacg acacccacat ccaaggtgaa gctctgctca actggggcag
      661 ggacaagctc cacggtgtag tcattttgtc gaactttgcc tgccacccag acgctttcca
      721 aggggagtac ttcgcccaga aagccgcaga acttggactt cttgcctggc gctttgtctg
      781 cgatgccact gtggacttag ggggttttac aactagattc gaaaccatag cggttttgat
      841 cgagcagaac atgcagaaaa ctgcagccag aaaaggtcag ctggctgttg caaacacggg
      901 acatggggct ccggtttcct acagcgctgc ggtcccatct caaagccatg tttccccctg
      961 gcagcaaagc agggagactc ttcttccaac aaagagccag tcgccacgtc tagagattgt
     1021 ccaaaagccc aaactgaaac ccaagaccat cgtcacctgg ccgtacatgg gcgagcctct
     1081 gaaccttgct ttgaagcagc tggtcttcga tttgggagct ggagagtttg ccgtccctcc
     1141 caaagcagtc tccgaagaaa ccctgaagcg gggaagcgaa gatctcgctg agacttgctg
     1201 tccttatgct ctgatctcgg ggagccttga ggagactatt cttggcgttt acgaagaggc
     1261 aggacgcccc attgacgcta aaatcctcat gctccatggg gaaggcccct gtgccttcgg
     1321 ctggtacagc attgccctga aggaaaagct cccaaaaaga ctcaagcaag tgtcgccgga
     1381 gatctccctc gagtggttta ccacaggcat cgaccagtct gtcagctttt ggcaaagcct
     1441 tgcaagcttt tgcgacgaga agttcgaacg cctcctctct agctttaaaa gccccctctc
     1501 atttttacag ccaaagaacc tcggagacgt tctccccgtt gtccgcaatg ctctaacaaa
     1561 actcaccctt actgaaaaga tgcgcgggct ctacctgatg gcaagaccgt ttggtgggcc
     1621 agctgcagaa gctgcttaca tcacctcttt gaacagcctg gacgaggcga aaaacaccag
     1681 agacatgcaa aaaagctata gggatgcccg agagattttt gctgcgatta tcaaagacag
     1741 aaccgctcct caagtggtta tagtaggcga gatctacgtt gccattaccc cctatgccaa
     1801 ccgctacgct gtcgataggc tcctcggcga tgcaggcctg caggtcatcg aggggattac
     1861 cgtgtcccac tacttgaagt gttccctgcg tcctgcagct aaaagactca tgcaaaacct
     1921 gtctctgttt aagtacgcaa acaaaaaagg cctcaagctt tttgcccaag gcctaagaga
     1981 caaagatagt gctccttata tgaactttga agtcggtgga gacggtgttt tgagcgtcgc
     2041 ttcagcacgg agggctctcc actccggcaa ggacggcatt gtgcagatct atccattaaa
     2101 ctgcatgccg gaagtagttg ccaaaccggc gataagcgag cttgcagcaa tctacgaccg
     2161 gcccttcctc agcctgtctt tcaacaggga gacggacacc gaacgcctga tgacagaggt
     2221 cctcacgttc gccagactga tcaagaacgc tagaaaaacc tcaagttgag gaaagtcacg
     2281 gccaaaaccg ccactatgcc caagactcca agagttattt gagtcgtttt ctcgctatcg
     2341 ctacggagat acctgctaga cggcccgatg atcataccga taaggaaacc gcctaaaaga
     2401 ccaccgacgt gcgaccaaac atcgatgttg gaaccgggaa cgagggcgat caggaagttt
     2461 atggcaagaa cagtgagaag cgaagactgg atggccggag tgacgtggga gtagcggaaa
     2521 actctcgcat agaggacaaa gcccaagagc ccgaacagc
//
LOCUS       DAOSDA010000265         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_26438_length_2556_cov_2.854058, whole genome shotgun sequence.
ACCESSION   DAOSDA010000265 DAOSDA010000000
VERSION     DAOSDA010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26438_length_2556_cov_2.854058"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLW82_06460"
     CDS             <1..306
                     /locus_tag="PLW82_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ46952.1"
                     /translation="QEILEYIDKNLSSKITIQDIASAVHLSPSHLSSLFKQKTGLTVY
                     SFILSKRLILARKLLMETDMLLDDIAEECGFSDVSSFIRCFRERVGTTPGQYRRSSR"
     gene            349..1671
                     /locus_tag="PLW82_06465"
     CDS             349..1671
                     /locus_tag="PLW82_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOJ46953.1"
                     /translation="MAKIALMGAGSVVFAKQLTYDIIARPEMRDAVISLHDIDPDRLD
                     TAEKVTRQLVEKLGGHATVEASLDRKKSLVGADYVIVMVQVGMFPATKIDFEIPRKYG
                     LKQTIADSYDVGGIFRFLRSYPFYKGLLDDMAEVCPDAYILNYTNPMAMNMLGIFKTA
                     PQVKAVGLCHSVQGTAGQLARLLDVPYEEIEYRVAGLNHQAFFLSLKHKGQDLYPRLK
                     ELVSDPEKVKDIPEKYDFMTKWDAVRVELFRRLGYYITESSEHNAEYTPYFIQHEELI
                     EKFNIPIDEYIRRCIWILDEYARIRKLVNEGKELEVHLSHEYAGNIIHALHTGQTFAF
                     NGNVLNTGLITNLPQNACVEVPCLVDKNGVQPTYIGDLPIQCAALNRPLASSVELAVE
                     AALTGKKEYVYQAVMMSPHSSSVLTLDKIWAMCDELIEAHGDALPKLS"
     gene            1709..>2556
                     /locus_tag="PLW82_06470"
     CDS             1709..>2556
                     /locus_tag="PLW82_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOJ46954.1"
                     /translation="MQNLFRPSHMVSRSICAENPTGGKGMGGRAVEGTGAHAARDLGV
                     GWKVNPYLILQPNEETVLADIKGPGIIEQIWATPMGPWRNLILRIYWDDQEHPSVECP
                     FGDFFGLGWGKFRQISSLPVCVNPGSAFNSYWPMPFKKRCRVTITSLNEHPHNFYYQI
                     NYALGEIPEDSLYFHAQFRRTNPLPYKSVYTIVDGIKGRGQYVGTSMSWGSNNSGWWG
                     EGEIKFYLDGDKDFPTICGTGTEDYFCGSYNFENQETKQYQEYSTPYAGFFVLKPDGL
                     YQSQTR"
BASE COUNT          660 a          667 c          635 g          594 t
ORIGIN      
        1 caggaaattc tcgagtacat cgacaagaac cttagcagta agattaccat tcaagacatt
       61 gccagcgctg tgcacctaag cccgtctcat ctcagctcac tgttcaaaca gaagacaggg
      121 cttacggtct acagtttcat cctgtccaaa cggttgattt tagcgagaaa gcttttgatg
      181 gagacagata tgctcttaga cgatattgct gaagaatgcg gcttcagtga tgtctcgagc
      241 tttattcgct gttttagaga acgtgtcggt actacccccg ggcagtatag acgcagttca
      301 agataactaa ggtcaatatt tatgatttac taaggaggag aagtttatat ggctaagatt
      361 gcattgatgg gtgcaggcag cgttgtgttt gcaaaacagc tcacctacga tatcattgcc
      421 cgccctgaaa tgagggacgc tgtgatctct ctgcatgaca ttgatccaga tcgccttgac
      481 actgctgaaa aggtcaccag acagctggtg gaaaaactcg gcggtcacgc tacagttgaa
      541 gcatctctcg accgcaagaa atcccttgtg ggcgcagatt acgtcattgt tatggtgcag
      601 gtgggtatgt tccctgcaac caagatcgac ttcgagattc cacggaagta cggccttaag
      661 cagacgatcg ccgatagcta cgatgtcggc ggcatcttcc gcttcctcag gtcttatccg
      721 ttctataagg gacttctaga cgacatggca gaggtctgtc cagacgccta catcctcaac
      781 tacaccaacc ccatggccat gaacatgctg ggcatcttca agaccgctcc tcaggtgaaa
      841 gctgtaggac tctgccatag cgttcagggc acagccggac agctggcaag acttctagac
      901 gtaccttatg aagaaatcga gtacagagtt gcaggactca accaccaggc tttcttcctg
      961 agccttaagc acaaaggcca ggatctctat ccgagactga aagaactggt ttccgatccg
     1021 gaaaaggtta aagatattcc tgagaagtac gacttcatga ccaagtggga tgctgtaagg
     1081 gttgaactct tcagacgcct gggctactac atcacagagt ccagcgagca caacgccgaa
     1141 tacactccgt acttcatcca gcacgaagag ctgatcgaga agttcaacat tcctatcgat
     1201 gagtatatcc gccgctgcat ctggattttg gatgagtacg ccagaatccg taagctggtc
     1261 aacgaaggga aagaactgga agtccacctg agccacgagt atgctggaaa catcatccat
     1321 gcactccata ccggccagac ctttgctttc aacggcaacg ttttgaacac cggtcttatc
     1381 accaacctgc cgcaaaacgc ttgcgttgag gttccctgct tagttgacaa aaacggcgta
     1441 cagccaacct acattggcga cctgccaatc caatgcgcag cgttgaacag acctctagca
     1501 tccagcgttg agctggcagt agaagctgct ctcacaggca agaaggaata cgtctatcaa
     1561 gcagtgatga tgtcgccgca ttcctcgtcg gtcctcacac tggataagat ctgggcgatg
     1621 tgcgacgagc tcatcgaagc ccacggcgac gccctgccaa aactttcata acgacaggga
     1681 ggttttcaga ttgacccttg cttttggaat gcagaacctt ttccggccat cgcatatggt
     1741 atctcgttcc atctgtgcgg aaaaccccac cgggggtaag ggtatgggtg gtagagcagt
     1801 ggaaggaacc ggcgcccatg cagcaagaga cctaggtgtg gggtggaagg tcaatcccta
     1861 tctgatcctt cagcctaacg aagaaacggt ccttgcagac attaaaggcc ccggcatcat
     1921 cgagcagatc tgggccacgc ccatgggacc ctggcgcaac ctgatcctta gaatctactg
     1981 ggacgatcaa gagcatcctt cggtagagtg tccttttggc gacttctttg gactgggttg
     2041 gggcaagttc aggcagatct cctctcttcc agtctgcgtc aaccctggta gtgctttcaa
     2101 cagctactgg cccatgccgt ttaaaaaacg ctgcagagtc accattacca gtctcaatga
     2161 acatccccac aacttctact atcagatcaa ctacgctttg ggcgagattc cggaagacag
     2221 cctgtatttc cacgctcagt tccgcaggac gaacccgctg ccgtataaga gcgtctatac
     2281 catcgttgat ggcattaagg gcagaggtca atacgttggc acctctatga gctgggggtc
     2341 gaacaactcc ggttggtggg gtgaaggcga aatcaagttc taccttgatg gcgacaagga
     2401 cttccccacc atatgcggta ccggtactga agactacttc tgcggttcgt acaacttcga
     2461 gaaccaggag accaaacagt atcaggagta ttccactccg tacgcaggtt tcttcgttct
     2521 gaagcccgat gggctttacc agtcgcagac caggtt
//
LOCUS       DAOSDA010000266         2546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_26567_length_2546_cov_3.101164, whole genome shotgun sequence.
ACCESSION   DAOSDA010000266 DAOSDA010000000
VERSION     DAOSDA010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26567_length_2546_cov_3.101164"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLW82_06475"
     CDS             complement(<1..376)
                     /locus_tag="PLW82_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ46955.1"
                     /translation="MTKSVDLEVFHGLILDDFQKQAIAALLEGKSVLVSAPTGVGKTL
                     IADYLIEEAQKKNKEIVYTAPIKALSNQKFKQFKNLFGEDQVGIITGDVVINSNAPIK
                     IMTTEIFRNILHSRPDDFAHASH"
     gene            517..1023
                     /locus_tag="PLW82_06480"
     CDS             517..1023
                     /locus_tag="PLW82_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46956.1"
                     /translation="MNKNRKLITILSVLALIAVILTGCGKKDEPKSDANAGASQPAPA
                     QQPTTSQPALSQSEMDQFRASLGLMSRSSYEDLEGVPLYVGSAEIDGSRSLTGSTLTA
                     SYSIIAKVDEFTTFYERELPNFGWSIVRKQVTDDSVRIEATKSGRLASIAASIVDGQI
                     KEYKIITD"
     gene            complement(1231..2049)
                     /locus_tag="PLW82_06485"
     CDS             complement(1231..2049)
                     /locus_tag="PLW82_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46957.1"
                     /translation="MDYSAKCYLKDNIEIAPRGSRWQKVVQLQPSLWRKGEKKLDLSF
                     LPPEPYTFVVADSGYDVALVKEALAKIILYLNDRGISTTDLAVRIEGKADVQADEYRK
                     VLGSWVYGSLKIDSGNEKRKRLLVAQCGLNPKIQHDLAVFVVPNGNGEIQLLTDDRNL
                     ANKEYDAQCGSDIPISDLVVAYDSAMKDAAPFGSKERTAHMVKKLKQLLTPGGHAVLL
                     CEEGAFGVYENVIVAPKDGYKAAMEEIEREVPEAFPQVTLIYDLAYTKIRNVLV"
     gene            complement(2199..>2546)
                     /gene="rpsI"
                     /locus_tag="PLW82_06490"
     CDS             complement(2199..>2546)
                     /gene="rpsI"
                     /locus_tag="PLW82_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOJ46958.1"
                     /translation="VARVRVIPGQGNITINGKTLEDYFGRKVLQTIVKQPLVLLGVEG
                     KYDVIANVDGGGNSGQAGAIRHGIARALQVADPSYRPSLKKAGFLTRDPRMKERRKYG
                     LKKARKAPQFSKR"
BASE COUNT          626 a          637 c          596 g          687 t
ORIGIN      
        1 catgactggc atgggcaaaa tcatcgggtc tggagtgcag gatgttcctg aagatctccg
       61 tggtcatgat cttgatagga gcgttgctgt tgattacaac atcgccggtg atgatgccaa
      121 cctggtcctc accaaagagg ttcttgaatt gcttgaactt ttggttgctc agagccttga
      181 tcggcgccgt gtaaacgatt tccttattct ttttctgggc ttcttcgatc aggtagtctg
      241 caatgagggt cttccccaca cctgtaggtg cagacaccaa tacggatttg ccttcaagta
      301 gcgcggcgat cgcctgcttt tgaaagtcgt ccagtattag tccgtggaat acttctaaat
      361 caaccgactt tgtcaaagtt ctgcctcctc tgctaccatg ctctgtttaa ttctatcaca
      421 tcggctggaa gcttactact ttctctccag attgaggtta acgcaggaga aaaacatggt
      481 atacttaagt tgcaagtaca atttgggggg tcagatatga ataagaacag gaaacttatc
      541 accattctca gcgtcttagc ccttatcgcc gttatactta cagggtgtgg gaaaaaggac
      601 gagccaaaga gcgacgccaa cgccggtgca agtcaacctg ccccagctca gcaacccact
      661 acttcacagc cagcgctgag tcagagcgaa atggaccagt ttagagcgag cctaggcctg
      721 atgtcaagaa gcagttacga agacttagaa ggggtacccc tttacgtagg ttctgctgag
      781 attgacggtt cgcgctcttt gacaggtagt actttaaccg cttcatacag tattattgct
      841 aaagttgatg aatttactac gttctacgag agagaacttc cgaactttgg ttggagcatc
      901 gtaagaaagc aggttaccga tgactcggtg cggatcgaag ctaccaagtc gggcagactt
      961 gcgtcaatcg ctgcttctat agtagatggc cagatcaagg agtacaaaat cattacggac
     1021 tagccaggcc atcaaaccta tatccgtctt gtgctgagct gctggaactt ccagcagctc
     1081 ttttcttttt ccctaagcaa gatgatgtta aaaataaggc ctcgtcaagg gttttccatg
     1141 tcgcatacag tccgacccca aacctcggcc aacgtcagcg ctccagcaca aacaaaaaag
     1201 caagcatcaa ccgcaatgct tgctttaaac tcaaacgagt acgttgcgaa ttttcgtata
     1261 cgcgagatcg taaatcaaag tcacctgtgg gaaagcttct ggaacctccc gttcaatctc
     1321 ttccatagct gctttgtagc cgtctttggg agcaacaatg acgttctcgt aaactccgaa
     1381 agcgccttct tcgcagagca aaacagcgtg tcctccaggt gtgagaagct gcttcagctt
     1441 tttcaccatg tgtgcggtac gctctttgct tccaaaaggt gcagcatctt tcatggccga
     1501 gtcataggcg acaaccaagt cactgatggg aatgtcagag ccgcactggg cgtcgtattc
     1561 cttgtttgcc agattccgat cgtctgtcaa aagctgaatc tcgccgttgc cgttaggcac
     1621 cacgaacacc gccaagtcat gttggatctt ggggttgaga ccgcactggg caactagaag
     1681 ccttttcctt ttttcgtttc ccgagtcgat tttaagactg ccgtagaccc aactgccgag
     1741 aactttgcgg tactcgtcag cttgtacgtc ggccttaccc tctatcctga cggcaagatc
     1801 agtggtgctg atgcctctgt catttaagta gaggataatc tttgcgaggg cttctttcac
     1861 caaagccaca tcatacccgg aatcggcaac tacaaaggtg tagggctctg gaggcaaaaa
     1921 ggaaaggtct agtttctttt ctcccttacg ccagaggctg ggctggagtt gaacgacctt
     1981 ctgccacctg ctcccacgag gcgcgatttc gatattgtct ttgaggtagc actttgcact
     2041 gtagtccata agaatctcct tactccacat ttagtatcga ggttactgca gatcgaactg
     2101 aaaacgttaa taggtagttt gatgtcttct gcagagctat cttcctcttc agggcaaaaa
     2161 aaagcagcag aagcatacgc tcctgcagca tcttttgttt agcgttttga gaattgagga
     2221 gccttacgag ctttcttaag accgtacttg cggcgctcct tcatgcgtgg atcgcgggtc
     2281 aggaagcctg cctttttcaa ggatgggcga taagacggat ctgctacctg cagggctctt
     2341 gctatgccat gtctgattgc gcctgcctgg ccactgttcc ctccaccatc gacattagca
     2401 ataacatcgt atttaccttc aacgccaagt aacaccaaag gctgctttac gatggtctgc
     2461 aaaaccttgc gaccaaagta gtcttccaag gtttttccgt ttatagtaat gtttccctgg
     2521 cctggtatta ctctaactcg agcaac
//
LOCUS       DAOSDA010000267         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_26583_length_2545_cov_2.776305, whole genome shotgun sequence.
ACCESSION   DAOSDA010000267 DAOSDA010000000
VERSION     DAOSDA010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26583_length_2545_cov_2.776305"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLW82_06495"
     CDS             complement(<1..383)
                     /locus_tag="PLW82_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870653.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HOJ46959.1"
                     /translation="MKRKVGITETILRDAHQSFMATRMSTPEMVEVAEKLDKIGYHSL
                     EVWGGATFDSSIRYLNEDPWERLRTLRKLIKNTKLQMLLRGQNLLGYKPYPDDIVEKF
                     VEKSIENGIDIIRIFDALNDIRNLET"
     gene            complement(398..1525)
                     /locus_tag="PLW82_06500"
     CDS             complement(398..1525)
                     /locus_tag="PLW82_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOJ46960.1"
                     /translation="MEKLIGFWESSGLANLTFGNLFMIALSCLLFYLAIKKDYEPLLL
                     IPIGFGMLLVNLPLAGLVAEPTATEPGGLLYYLGLGVQYGIYPPLIFLGIGAMTDFGP
                     LIANPKSMLLGAAAQIGIFITFLGALLLGFTPQQAASIGIIGGADGPTAIFLTTKLGP
                     ELLGPIAIAAYSYMALVPIIQPPIIKLLTTPEERKARMGAIRQVSKTEKIVFPIMVTI
                     VGTLIVPSAGPLLGMLMLGNLLRETGVTDRLSDTAQNALINIVTIFLGISVGTKASAD
                     KFLSFDFFKILFLGLFAFATATACGVLLGKLMYKLTKGKINPMIGAAGVSAVPMAARV
                     VQKLGQEEDPKNHLLMHAMGPNVAGVIGSAVAAGMLLALLG"
     gene            complement(1547..1891)
                     /locus_tag="PLW82_06505"
     CDS             complement(1547..1891)
                     /locus_tag="PLW82_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOJ46961.1"
                     /translation="MKKEYIVTVNGKSYEVTVSEKSGSPVVEKVSEAVVKEEPTLQAT
                     GTPVVAPMGGLVKDILVKVGDKVEDGTVVAIVEVMKMDTEVTAESAGVVESIQVAKGD
                     NLDSGATIMTIK"
     gene            complement(1907..2146)
                     /locus_tag="PLW82_06510"
     CDS             complement(1907..2146)
                     /locus_tag="PLW82_06510"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOJ46962.1"
                     /translation="MENLVFGLEVSFVSLIVVFLTLYLLSLLIDLVRVFVKDKPKEKA
                     STTTLQKAAPRADLDDDGELIAVVAAAIQAYESNR"
     gene            complement(2263..>2545)
                     /locus_tag="PLW82_06515"
     CDS             complement(2263..>2545)
                     /locus_tag="PLW82_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOJ46963.1"
                     /translation="QNITLSETGEEKLYVGVCVGTSCYLKGSYDTMQQLRELIAAEDL
                     ADKVSIEATFCFENCAGAPSVKIGDEIINASSYKPQELVALIKNKLALV"
BASE COUNT          618 a          667 c          594 g          666 t
ORIGIN      
        1 gtttccaggt tgcggatatc gtttaaggcg tcgaagattc tgatgatatc gataccgttt
       61 tcgatgcttt tttcgacgaa cttctccaca atgtcgtcgg gatagggttt gtagccgaga
      121 aggttctgcc ccctgagaag catttggagc ttggtgtttt taatcagctt tctcaaagtt
      181 ctcagcctct cccaagggtc ttcgttgagg tagcgaatgg agctgtcaaa cgttgcacca
      241 ccccagacct cgagggagtg atagccaatt ttatccagct tctctgcgac ttccaccatc
      301 tccggtgtgg acatgcgggt agccataaaa gactggtgcg catcacgcaa aatggtctcg
      361 gttataccaa cttttctttt caagtaatta cctcccttta tccaagtaat gcaagaagca
      421 tccctgctgc aactgccgag ccgatcaccc cagccacgtt gggacccatg gcatgcatca
      481 ggaggtggtt cttcggatct tcttcctgcc cgagcttctg cactactctg gcagccatcg
      541 gcacagcgga cactcctgcg gcaccgatca tcggattgat cttgcctttg gtaagcttat
      601 acatcagttt gcccagcaaa accccacagg ctgtggccgt agcaaaggca aacagaccga
      661 ggaataggat cttgaagaag tcgaaactga ggaacttgtc tgcggatgcc ttagttccca
      721 cagagattcc caaaaagatg gtcacgatat tgatgagggc gttttgggcc gtatcgctca
      781 accgatcggt gactcccgtc tctctgagga ggttccccag catcagcata ccgaggagcg
      841 gtcctgcaga cggtacgatc aaggttccca caatcgtcac catgatcggg aagacaatct
      901 tctctgtctt gctcacctgt ctgatggctc ccatgcgcgc tttgcgttct tctggagttg
      961 tcaggagctt aatgatcggt ggctgaataa ttggtacaag tgccatatag gagtaggcag
     1021 caatggcgat ggggcctaga agctcaggtc ctagtttcgt tgtaagaaaa atcgccgtag
     1081 gtccatctgc accgccgata attccgatag aagctgcctg ctgaggggtg aatcccaaaa
     1141 gcagagcgcc taaaaacgta ataaaaatgc cgatttgcgc ggctgctcca agcagcattg
     1201 acttgggatt ggcgatcagc ggaccgaagt cggtcatagc gcctataccc aaaaagatca
     1261 gcggaggata gataccgtac tggaccccca gtcccagata gtacaaaagc cctccaggtt
     1321 ctgtggctgt aggttcggca accagaccag ccaaggggag gttgacaagg agcattccga
     1381 agccgatggg aatcagcaaa agcggctcgt agtccttctt gattgccaag tagaacagca
     1441 gacatgacag ggcaatcatg aacaggttgc caaaggttag attagccaga ccggaacttt
     1501 cccaaaaacc gattagtttt tccatattct gtcacctgtc tgttatttat ttaatcgtca
     1561 tgatcgtcgc accactgtcg agattgtcgc cttttgcgac ctggatggat tcaactaccc
     1621 ctgcgctttc cgcagtcact tcggtatcca tcttcataac ctcaacaatg gccacgacag
     1681 tgccgtcttc gactttgtca ccgactttta ccaggatgtc tttaaccagc ccgcccatag
     1741 gcgccacaac cggcgttccc gtagcctgta gggtcggttc ttccttcacc accgcttcac
     1801 tgaccttctc cacaaccgga ctccccgatt tctcgctgac tgttacttca tagcttttgc
     1861 cgttgacagt cacaatgtac tctttcttca taagctcagt ctccttttac ctgtttgatt
     1921 cgtaagcttg tatggccgcc gcgacgactg caataagctc gccatcgtca tctaagtctg
     1981 cacgtggcgc tgctttttgc agtgttgtag tgctggcctt ttctttaggc ttatctttga
     2041 caaaaaccct taccaagtcg ataaggagag acagtagata gagggttaag aacaccacta
     2101 ttaagctgac aaatgagacc tcaaggccaa aaactaggtt ttccattcga tcacctcgct
     2161 gcaactgggt taacatactt actctatttc tagcttcaaa gcggatatcc ttgccaaaca
     2221 acaaataccc gaaataacgc cggggaaacc cccggcgtta ttttatacca aagctagttt
     2281 attcttgata agtgcgacta actcctgcgg tttgtaagaa ctggcgttaa taatttcatc
     2341 accgatttta accgacggag ctcctgcgca gttttcgaaa cagaaagtag cttcaatgct
     2401 cactttgtca gcgagatctt ctgctgcgat gagttctctc agctgctgca tagtgtcgta
     2461 ggaacctttg aggtagcagg aagttcccac acaaacccca acgtagagct tttcctctcc
     2521 agtttcgctg agagtgatgt tttga
//
LOCUS       DAOSDA010000268         2541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_26634_length_2541_cov_3.117458, whole genome shotgun sequence.
ACCESSION   DAOSDA010000268 DAOSDA010000000
VERSION     DAOSDA010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26634_length_2541_cov_3.117458"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PLW82_06520"
     CDS             complement(<1..1241)
                     /locus_tag="PLW82_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46964.1"
                     /translation="MKKGLLCVLVVLITSVMILAAPVVVWSSLSVEKNRIMQEMTDSV
                     FTAKTGIEVKIESIPSDGRESKYLLAAASGDVPDIGVTGSLGPADLGVRGAAVDLRER
                     FGEEYLAIKREMYPGLMRSFDFMGTDFGLPLDIALYPLGYRSDILSDMGLSVPTTWQD
                     LYQMLPKLQANNMNFSTAFGFEGTGNTVYADVSMFIWQHGGDWYTEDRTRSGLDSPES
                     IAGFIEFTELYTKHNVPKAVNTYMGFQSGELPLIQLTNWWYSSFLFSMPELQGKWGVT
                     VVPGTVREDGSISHAAYIGGNTFVMFKDGKQIDNAFKWLKWWLSADTQSQFATIVPEK
                     IPGAIVIPPNIHSIQSIPLPEEHMEAYMKQAQHSIAPAYALAPESITHRFINNACAKV
                     VLYNEDPVDAILEAAREMNVEL"
     gene            complement(1386..>2541)
                     /locus_tag="PLW82_06525"
     CDS             complement(1386..>2541)
                     /locus_tag="PLW82_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46965.1"
                     /translation="DSFPALSKDETKLYFQSNRTGCYGIWVLDLQTMQIAPVATNHYG
                     AFKPTPSLDTENRVAFVSYTNDGLRLAIHEFSGAASWQQLTIESPPERKQYPSLDELL
                     AQDGYSLKKYRSSSYAKPTMWLPSFVGALVLGTDPLQNYVYTALYNYNPFAEQGVSRH
                     EFAAAVDLIVNPLHVVSLSVESNFKKTYFSTIYSGTVPLGSNALLLSGSANTIGDFAT
                     EVRLQLPWGTKAATGTSFIFGKASVELWQKTLDNYEFNLGYSHKLQASHDVNFELSFS
                     YNFNHDGYVTETNTYLGEHIAFSEYAMNWVFARPLWSVGASSTVWSEKMTLSPKFAFM
                     LGDKNWDLSFGLQFTNTISLTYGRLPIDLGLKVMYYMGSNKLIVSPVIRL"
BASE COUNT          633 a          615 c          649 g          644 t
ORIGIN      
        1 agttcgacgt tcatttcccg tgctgcttcc aaaatggcgt ctactggatc ttcattgtaa
       61 agcacgactt tggcgcaggc gttattgata aacctgtgag ttatagattc tggagccagg
      121 gcatatgccg gagcgatgct gtgctgagcc tgtttcatgt aagcttccat gtgctcctca
      181 ggaagaggaa tgctttggat tgagtggatg tttggtggaa tcacgatggc tcctgggatc
      241 ttttccggaa ctatcgtggc aaactgcgat tgagtatcag cagacaacca ccacttaagc
      301 cacttgaagg cattgtcgat ctgctttccg tccttaaaca tcacaaatgt attgcctccg
      361 atgtaggccg catggctaat ggagccgtct tctctcaccg tccccggtac aacggttact
      421 ccccacttgc cctgcagttc gggcatggaa aagaggaacg aagagtacca ccagtttgtc
      481 agctggatga gcggtagttc gccggactga aagcccatat acgtgtttac agccttcggt
      541 acattgtgtt ttgtatagag ttcggtaaac tcgataaaac cagcgatcga ttctggagag
      601 tccaaaccgg acctggtgcg atcttctgtg taccaatctc caccgtgctg ccagataaac
      661 atggagacat ccgcatacac cgtgttgccc gtgccctcaa agccaaaggc agtggagaag
      721 ttcatgttgt tggcctggag tttggggagc atctggtaca gatcctgcca ggtagtgggt
      781 acagacagac ccatgtcaga aaggatgtcc gatctgtagc ccaggggata cagtgcaata
      841 tccaggggca gaccgaagtc ggtgcccatg aaatcaaagg accgcatgag tccgggatac
      901 atctctcgct tgatcgccag atattcctcg ccaaatctct ctctgagatc cactgcagca
      961 ccgcggacgc cgaggtctgc aggtcccaaa gaaccagtga ctccgatgtc tggaacatct
     1021 cccgaagcag cagcaagtag atacttgctt tctctgccgt cagaaggaat actttcaatc
     1081 ttgacttcga ttcccgtttt ggctgtaaag actgagtcgg tcatctcctg cataatgcgg
     1141 tttttctcta cagaaagaga cgaccagacc acgacaggag ctgccaagat cattaccgat
     1201 gtgattagca ccacaagaac acacaacaac cctttcttca tcttccaccc ctccaatgaa
     1261 tttgtgcaga ataaaagcaa cttgcagttg attactactt tctgctactc ccccaaaagt
     1321 ccttgaaacg ctgctttttt tcagcgtata aaaaaagcgc ggctgtttac tgccgcgcca
     1381 gtatcctaga ggcggataac cgggcttaca attaacttgt tgcttcccat gtaatacatg
     1441 actttcagtc ccaaatcgat aggaagtctg ccgtaggtga gacttatggt gttggtgaac
     1501 tgaaggccga agctaaggtc ccagtttttg tcgcctaaca tgaaagcgaa ctttgggctg
     1561 agagtcatct tttcggacca tacggtcgat gatgcaccga cagaccaaag gggtcttgca
     1621 aacacccagt tcatagcgta ttcggagaat gcaatatgct ctccgaggta ggtgttggtt
     1681 tcagtgacat agccatcgtg attgaagttg taggagaagg aaagctcaaa gttcacgtcg
     1741 tgacttgctt ggagcttgtg gctgtaaccc aggttaaact cgtagttgtc cagagtcttt
     1801 tgccacagtt ccacgctcgc ctttccgaaa atgaagcttg tgcccgttgc tgctttcgta
     1861 ccccatggaa gttgaagacg gacttcggtt gcaaagtccc caatggtgtt agcagagcct
     1921 gaaaggagca gggcattact accaagcgga accgttcctg aatagatggt actgaaatag
     1981 gtttttttaa aattggactc tacggacaga gaaaccacat gcaggggatt gacgattaag
     2041 tccaccgctg cggcaaactc gtgcctcgat acgccttgct ctgcgaaggg gttgtagtta
     2101 tacagcgcgg tatatacgta gttttgcaga gggtcggtgc cgaggaccag agcgcctaca
     2161 aacgaaggca accacatcgt gggtttggcg tagctcgaac tgcggtactt cttcagggag
     2221 taaccgtctt gagccaagag ctcatcgaga gatggatact gctttctctc agggggactt
     2281 tctatcgtca actgctgcca cgaagcagct ccggaaaact catggatggc tagccgaagg
     2341 ccatcattgg tgtaggagac aaaagccact cggttttcgg tatctaggct aggtgtgggc
     2401 ttgaatgctc cgtagtggtt tgtagccaca ggtgctatct gcattgtttg tagatctaaa
     2461 acccagatgc cgtagcatcc ggtacggttc gactggaagt acaacttggt ttcatctttg
     2521 ctcaaagcag ggaacgaatc a
//
LOCUS       DAOSDA010000269         2534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_26739_length_2534_cov_2.536103, whole genome shotgun sequence.
ACCESSION   DAOSDA010000269 DAOSDA010000000
VERSION     DAOSDA010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26739_length_2534_cov_2.536103"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLW82_06530"
     CDS             complement(<1..286)
                     /locus_tag="PLW82_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOJ46966.1"
                     /translation="MITANQLRKMYLNFFEEKAHKALPSFSLIPKSDPTLLLTGAGMV
                     PLKPYFLGEAKPESGRLTTCQKCMRTGDLENVGKTARHLTFFEMLGNFSFG"
     gene            complement(335..1366)
                     /locus_tag="PLW82_06535"
     CDS             complement(335..1366)
                     /locus_tag="PLW82_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOJ46967.1"
                     /translation="MQRKLLLLLGLGALVVVFLVVYLLPLVLPLVLGLILAYLLEPLV
                     TSLERRQVPRYVGIVIVYASVLICLGLLGLYIVPALFEQISEISAILPERLLYLKEHT
                     LNFALRLTGGKGESYIKGFLDEISRKTYEFAVALGAQVVASFPRLLHTLFIVFVSLVL
                     SYYFLSDRQRFVQGVYGIFPREHTQVVQGVLLEINRSLGGYLRGLLTVAFLVGFLAFL
                     AMVILRIPYALLIGVIVGFTELIPYFGPVIGAIPGVLLGFLASPTKGLWAAVTFAAIQ
                     QIEGLFLTPKIVGSHSDLHPVTVILAVIGGEMLWGFGGMIIAVPLVAVLKSAALAIYE
                     RMVRARLID"
     gene            complement(1410..1592)
                     /locus_tag="PLW82_06540"
     CDS             complement(1410..1592)
                     /locus_tag="PLW82_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46968.1"
                     /translation="MRFWQGMVLGIAVGLVGVVLYNRSSTSTKAALRRRAQEAGELIG
                     TAARSVRVGMVNTNNK"
     gene            complement(1593..2150)
                     /locus_tag="PLW82_06545"
     CDS             complement(1593..2150)
                     /locus_tag="PLW82_06545"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOJ46969.1"
                     /translation="MQRLSSLIEAKVYDLEGGRIVGRVEDVAFSLQTGTIYGIFVKRS
                     LQRKRHGFVPIASVKGIGSDAVTILSLDCVEKGVAKPKGLLGQKVMTRQGNTVGTVTD
                     AIISKDERLVIGLELAQSFWEDLRHRRKIWPLSPDFVFGDAVIAPENYRSVAPEKLLE
                     HARRLAPFSLQLENEEVCFLNRRDR"
     gene            complement(2234..>2534)
                     /locus_tag="PLW82_06550"
     CDS             complement(2234..>2534)
                     /locus_tag="PLW82_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOJ46970.1"
                     /translation="IIVDAKFKPFGCGAAIATSSMITEMIIGKSIEEAGNISNKAVAE
                     ALGGLPPVKMHCTLLAADALRRAIYDYKKKNGLPVDDCDDCDTCCHDHHDIEEAF"
BASE COUNT          681 a          673 c          586 g          594 t
ORIGIN      
        1 tgccaaaaga gaagttcccc agcatttcga aaaaggtcag atgccgggcg gtttttccaa
       61 cattttctaa gtcgccggtg cgcatgcact tttggcaggt cgtcagtctg ccagattctg
      121 gttttgcttc gcctaaaaag taaggtttta acggtaccat accagctcct gtaagaagca
      181 gggtaggatc cgatttcgga atcaaggaaa aactaggaag tgctttgtgt gctttttctt
      241 caaaaaagtt taaatacatc ttcctcagct gattcgctgt tatcataaag tcacctccgg
      301 tgtatctttt ctaatcatac taattcgtgt gaaattagtc aatcaatctg gcacgcacca
      361 tcctttcgta gatagcaaga gctgcgcttt tgagaactgc caccagagga actgcaatga
      421 tcatcccgcc aaagccccag agcatctcgc ctccgattac cgcaagaatc accgtgacag
      481 ggtgcagatc cgaatgactg cccacgatct tcggcgtgag aaacaaccct tcgatctgct
      541 ggatggcagc aaaagtcact gccgcccaaa ggcctttggt gggagaagct aaaaagccca
      601 agagcacacc gggaattgcc ccaatcaccg gtccgaagta gggaatgagt tcggtaaagc
      661 ccacgatgac gccgatcagg agagcatagg gaatgcggag aatcaccatg gctaaaaaag
      721 ccaaaaaccc cacaagaaac gcaacggtca gaagacccct gagataccct ccgagagagc
      781 ggttgatctc cagaagcact ccctgaacca cttgagtatg ttctcttgga aagatgccgt
      841 agacgccctg cacgaacctc tgcctgtcgc tgaggaaata gtaggatagc accagcgaga
      901 caaaaacgat gaaaagggtg tggaggagcc tgggaaacga cgctaccacc tgagctccca
      961 gtgcaactgc aaactcgtag gttttcctgg agatttcgtc caggaagccc ttaatatagc
     1021 tttccccctt tccccctgta aggcggaggg caaagtttaa agtgtgctct ttgaggtaaa
     1081 gaagccgctc cggaaggata gccgagatct cgctgatctg ttcaaacaaa gctggaacta
     1141 tgtaaaggcc taaaagccca agacagatca aaacagaagc gtagacgatg acaatgccga
     1201 catagcgcgg cacctggcgc ctttctaagg aagtaaccag gggctcgaga agataggcca
     1261 ggatcagccc cagaaccagc ggcagaacca ggggcagaag ataaaccact aaaaaaacga
     1321 caaccaaggc cccgagtcct agtaggagga gaagttttct ctgcatatga ttccgtccct
     1381 ttacggcaaa taagaggggc agccccctct tatttattat tggtatttac catgccaact
     1441 ctcactgatc ttgcagcggt accgatcagc tcgcctgctt cttgtgctct tcttctaagg
     1501 gctgctttgg tgctggtgct gctgcggttg tagagcacaa ctcccaccaa gcctactgcg
     1561 atgccaagga ccataccctg ccaaaaacgc atttatctat ccctcctgtt taagaaacaa
     1621 acctcttcat tttccagctg cagagaaaac ggggcaagcc ttcgggcatg ctccaaaagc
     1681 ttctctggcg ccacgctacg gtagttttcc ggtgcgatta cggcgtcgcc aaaaacaaaa
     1741 tccggcgaaa gcggccagat ctttcgcctg tgccttaggt cttcccaaaa gctttgggca
     1801 agttcgagac cgataaccag ccgctcatct tttgagatga tcgcatctgt tacagttccg
     1861 accgtattgc cctgcctagt catgaccttc tgacctaaaa gacccttggg ctttgcaaca
     1921 cctttttcta cgcagtctaa gctgagaatc gttacggcat cactacctat gcctttgacg
     1981 ctagcaatgg gcacgaaccc atgccttttc ctctgcagcg acctcttcac aaagatgccg
     2041 tagatagttc cggtttgcag agaaaaagca acgtcctcga ctcttcccac aatcctgccg
     2101 ccttctaaat cgtatacttt agcttcgatg agactgctta acctctgcat gactacccca
     2161 tacttataat gcactcaact gaaaaagaat aacccgcaag gaagaccttg cgggctgaaa
     2221 ccttttacca gtattaaaat gcttcttcta tatcatgatg atcatggcag caggtatcac
     2281 aatcgtcgca gtcatcaact ggcaggccgt ttttcttctt gtagtcgtag atggccctgc
     2341 gcagcgcgtc tgcagccaaa agcgtgcagt gcatcttcac aggaggaaga ccgccgagag
     2401 cttcggctac tgccttgttg gagatgttgc ctgcttcttc gatggacttg cctatgatca
     2461 tctctgtgat catcgacgat gttgcgatag cagctccaca tccgaaaggc ttgaatttag
     2521 catctacaat gatt
//
LOCUS       DAOSDA010000270         2520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_26933_length_2520_cov_3.397972, whole genome shotgun sequence.
ACCESSION   DAOSDA010000270 DAOSDA010000000
VERSION     DAOSDA010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26933_length_2520_cov_3.397972"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(5..196)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            374..1225
                     /locus_tag="PLW82_06555"
     CDS             374..1225
                     /locus_tag="PLW82_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ46971.1"
                     /translation="MDEAKRALHDEEFYRRRWRKRALQISIVVLIVLAIASYFGIKQL
                     NAIRAYKIGLQLMDEAQWEDAIVSFTKALSIRSEYPDASFKKAEALVTIGRYTEAKQT
                     LLDTNPDSINNKTLAANIYNLLGIISLRQGSLDEAVSYFNTAIDLNPTASSSASSYLG
                     LAEALVRQEKDLDKAVEYVNSAIALRPSPSMYTSLYNMQGRAYLAAGNYEAANQAFFE
                     SLSYNDNYVLSYYYLGVSFEATGNIAKAKGAYKRAVELNPDYDAAYDALQKLDAEYPD
                     IAPESVR"
     gene            complement(1277..2152)
                     /locus_tag="PLW82_06560"
     CDS             complement(1277..2152)
                     /locus_tag="PLW82_06560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOJ46972.1"
                     /translation="MILFLDLEMNTWKGNVGELNSEIIEIGAVLMDDELRIVDTFSEL
                     VRPTHQTKLSPYCLRLTGIDRKKLFRSLPLKDVLVDLEHWLDDYENIDSVYSWNESDY
                     RQLIKECSVKKISSPFIPFLENSYADFQKVFGMEFDWPTCGLSDAMKVFNIAHSGKAH
                     RALNDAMDLARLYKKMKKTKRDNRRFQLIKEQAELKKIERLNPVLKLMQRVRSGKESS
                     AEQDVKVRDAVLNAFKTDITKTLKKYKVREIVAVCPELKDWLLVLEGMLTELEDESAG
                     ESDAVGGLDSRAVNQ"
     gene            complement(2271..>2520)
                     /locus_tag="PLW82_06565"
     CDS             complement(2271..>2520)
                     /locus_tag="PLW82_06565"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HOJ46973.1"
                     /translation="GATTVRFDPYLSLQAYLWAKGASFEEILRIAPVPEGDIVASLRR
                     SIDILRQIRAAVQGDLSLTEKLTEAMKLIDRDVVSVNI"
BASE COUNT          649 a          642 c          523 g          706 t
ORIGIN      
        1 gcgaagaaga ttttttcccc taggcaggtt tcctgactca cagatcatcg gcatatctgg
       61 cagaccttcc cagcatacaa ccagtggctg ccacacctcc ctgcatacag tggcgcgacc
      121 gtgcagttta ccaagcaaag ttggcgctgc ttccctgtta tcccaacgtt tgggcaccta
      181 ggtgagtagt atttcctctt ttcctttcct ttctcctgct taatatcttt tttagtgctt
      241 ttcgttaaat ttcttttctc ccaacctcga atactcatgc aggaaaagcg tgtttcatat
      301 tgtataatac taaggagagg gtgctttata gtaccaacac aggttggaac gattttattc
      361 ggaggtgttt cttttggacg aagcaaaaag agctctacac gatgaggagt tttatcgccg
      421 cagatggaga aaaagagcct tgcaaatcag cattgttgtg ctaattgtac tggctatagc
      481 aagttatttt ggtataaaac agctaaatgc cattagagcg tataaaatag gtcttcagct
      541 gatggacgaa gctcaatggg aagacgcaat cgtatcattt actaaagctc tatcaatcag
      601 atctgaatat ccagacgcaa gtttcaagaa agctgaagcg ctcgttacta ttggccgtta
      661 tactgaagct aagcaaaccc ttctcgacac caatccagac agcattaaca acaagacgct
      721 ggccgccaat atttacaacc ttctcggcat catttcttta aggcagggca gcttggacga
      781 agctgtttcg tatttcaata ccgctatcga tttgaatcct actgctagtt cttctgctag
      841 ctcttacctt ggtctagccg aagctctagt ccgtcaggag aaagacttag acaaggcagt
      901 tgagtatgtc aactcagcta tagctctcag gccaagccca agtatgtaca ccagtcttta
      961 caacatgcag ggtagagcat atctggccgc aggcaactac gaagctgcaa atcaagcctt
     1021 cttcgagtct ctgtcataca acgacaacta cgttctgtcc tactactacc tcggcgtttc
     1081 ctttgaagca actggcaaca ttgccaaagc caaaggcgcc tacaagcgtg ctgtagaact
     1141 gaacccagac tacgatgcgg cctatgacgc actgcaaaaa cttgacgctg agtatcccga
     1201 tatagctcct gagtctgtac gttaaagcac aaacaactct gaaataaaag agcggaagca
     1261 tatgcttccg cttcttttat tggtttactg ctcgactgtc caatccgcca acggcgtcgc
     1321 tctcacctgc agactcatct tcgagttctg tcagcatgcc ctcgagtacc aagagccagt
     1381 ctttcagttc tggacagacg gcaacgatct ctctaacctt atactttttc agtgttttgg
     1441 tgatgtctgt tttgaaagcg ttcaaaacag catcccttac tttgacatcc tgttccgcac
     1501 tggactcttt tccagagcgg actctttgca ttagcttcag tactgggttc aacctctcaa
     1561 tcttcttcag ctctgcctgc tctttgatga gttggaatct gcgattgtcc cgcttggtct
     1621 tcttcatttt tttgtagagc ctggctaaat ccatggcatc gttcagagct ctgtgggctt
     1681 tgcccgaatg agcgatgttg aaaaccttca ttgcatcgga aaggccacac gtaggccagt
     1741 cgaactccat gccaaagacc ttctgaaaat ccgcgtaact gttttccaga aaaggtatga
     1801 acggcgaact aatctttttt accgaacact cttttatcaa ctgacggtag tcgctctcat
     1861 tccaggaata aacggaatct atgttttcat aatcgtctaa ccagtgctcc agatccacca
     1921 gaacatcctt tagaggtaac gaacgaaaaa gcttttttct gtctatcccc gtaaggcgca
     1981 ggcagtatgg cgacagcttc gtttgatgag ttggccggac tagttcgctg aaggtgtcta
     2041 caatcctcag ctcatcatcc attagcacag ctcctatttc gattatctcg ctgtttagtt
     2101 ctcccacatt gcccttccaa gtgttcatct ccaggtccaa aaagagaatc atcccgtgcc
     2161 tcctctcaaa caaacataaa aggtgataaa gactatcacc ttttaattct acatcacttt
     2221 cagttcacga gaaagtcaga aaccccttct tccataaacc acttgcagcc ttaaatattg
     2281 acagaaacca catctctatc gataagcttc atggcttcgg tgagtttttc tgtaaggctg
     2341 aggtctccct gcactgcagc gcggatctgg cgaaggatgt ctatgctccg ccgcagtgac
     2401 gcaacgatgt cgccttctgg aaccggtgca atgcggagaa tctcctcgaa gcttgcccct
     2461 ttagcccaga gatacgcttg gagcgaaagg taggggtcaa agcgtactgt ggtggcgcct
//
LOCUS       DAOSDA010000271         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_26961_length_2518_cov_3.921234, whole genome shotgun sequence.
ACCESSION   DAOSDA010000271 DAOSDA010000000
VERSION     DAOSDA010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26961_length_2518_cov_3.921234"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLW82_06570"
     CDS             complement(<1..751)
                     /locus_tag="PLW82_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ46974.1"
                     /translation="MKTILEGAREIPVKGSYDVIVVGGGTAGAIAGIAAAREGAKTLI
                     VEANGFLGGTATASLVTPLMGNHVDGKPLNTGIGLEVMQKLKETGDCGELNGNLGYFN
                     WDMLKFVLEDMYLDAGGELMYHAHFSAPIMEGNALKGIIVESKSGRFALLGKVIIDAS
                     GDADVAARSGAPIESGDETGKNQALSLRFSVANIDLDKFYQFLHSLDGYTTTPPFFHG
                     AHVNGNRYALTPFFEKAYEEGVLEVSDGDYFQ"
     gene            complement(791..1915)
                     /locus_tag="PLW82_06575"
     CDS             complement(791..1915)
                     /locus_tag="PLW82_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOJ46975.1"
                     /translation="MVKVFSGARILTAAAEDFEVGTLIVEDGKIKSVSAAEGDRDALP
                     YSGKVITPGLFDAHCHIGIYEEGIRDEGDDVNEMTDPVTPDVRAIDGIYHEDPAFAES
                     YEGGLTTFCVAPGSGNVVGGQVCLIKNRRDLPDKMLYKEYVGMKVAFGENPKRVYGNQ
                     KKKPMTRMGIAALLRETLTKAENYRNKQDKEKNLGMEALVKVLEGKVPLKAHAHRADD
                     ILTALRIAREFGVRITIEHCTEGDRILDALKEAGVPCIIGPSLSTRSKYELRYRNYST
                     AKVLSDAGILTAIMTDHPVIPAKELRVCAIAAIREGLDPKEALKMITINPAKIFGVDQ
                     EIGSLEPGKSADFVVWSGDPFDARTYVEELYVDGVLVYKN"
     gene            2140..>2518
                     /locus_tag="PLW82_06580"
     CDS             2140..>2518
                     /locus_tag="PLW82_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46976.1"
                     /translation="MPKRTSFLSFICLLFFAVGAVAAPISIWSPLHVEWNNIIQQLSD
                     LEFTPKFGIRVKVHSIPRSGYEQIYLLSAASGNVPEIGITSLIGATDLGVRGVTVDLS
                     QYGEEYLEIKQQIYPGSSRALSYN"
BASE COUNT          577 a          681 c          570 g          690 t
ORIGIN      
        1 actggaagta gtctccgtcg gatacttcca gaacaccttc ttcataagcc ttttcaaaga
       61 aaggtgtcag agcgtagcgg ttgccgttga catgagcccc atggaaaaag ggtggggtcg
      121 tggtgtagcc gtcaaggctg tgaagaaact ggtagaactt atcgagatcg atgttggcaa
      181 cgctgaagcg cagactcaag gcctggttct ttccggtttc atcgccagat tcgattggcg
      241 cgccgcttct tgctgcaacg tcagcatctc ccgatgcatc gataatcacc ttgccaagca
      301 gggcgaagcg tccggacttg gactcaacaa taatgccttt caaggcattg ccttccatga
      361 tcggagcgct gaagtgggcg tgatacatca gctcgccgcc tgcgtcaaga tacatatctt
      421 ccaagacaaa cttgagcata tcccagttga aatagccgag gttgccgtta agctctccgc
      481 agtcaccggt ttcctttaac ttctgcatta cctcaagacc gatgccggtg ttgaggggtt
      541 ttccgtcgac gtggttgccc atcagtggcg ttaccaagga cgcagttgcc gtaccgccta
      601 gaaagccatt ggcctctacg ataagcgtct ttgcgccttc cctagcagca gcaatgccgg
      661 cgattgcgcc tgcggtgcca cctcccacga ctatcacatc ataacttccc ttaacgggaa
      721 tctctcttgc accttcaaga atggtcttca ccgttttcgc ctcctcaaaa aacgctttaa
      781 gccaaatagc ttagttctta tataccagaa ctccatctac gtagagttct tccacgtatg
      841 tccttgcatc gaacggatcg ccggaccaga caacaaaatc tgcagacttt ccaggttcta
      901 gagaaccgat ttcctggtca acgccgaaga tcttagcagg attgatggtg atcattttca
      961 gtgcttcttt cggatccagc ccttccctga tagcagcaat tgcacatacc ctcagctctt
     1021 tggcgggaat gacgggatgg tccgtcatga ttgccgttaa aatcccggca tcgcttagca
     1081 ctttggcggt actgtagttt ctgtagcgca gctcgtactt ggaccgagtg gacaatgaag
     1141 gcccgatgat gcagggtaca ccggcctctt tcaaagcgtc aaggatgcgg tctccttcgg
     1201 tgcagtgctc aatggtgatc ctcacgccga actctctggc tattctcaaa gcggtcagaa
     1261 tatcgtctgc tctgtgcgca tgggctttaa gaggcacctt gccctctaag actttgacca
     1321 gcgcttccat gcccaagttc ttttccttgt cctgcttgtt cctgtaattt tccgctttag
     1381 tcagagtttc tctaaggagt gcggcgatgc ccattctagt catgggtttt ttcttctggt
     1441 tgccgtacac tctcttgggg ttttcgccaa aggcgacttt catacccacg tactctttgt
     1501 agagcatctt gtcgggaaga tcccttctat ttttgatcag gcagacctgt ccccctacaa
     1561 cgttgccgct tcccggtgca acgcagaaag tggtcagacc tccttcatag ctttcggcaa
     1621 acgccggatc ttcgtggtag atgccatcga tagcccttac atcgggcgtt accggatcgg
     1681 tcatctcatt gacatcgtca ccttcgtccc taatgccttc ttcgtaaatg ccaatatggc
     1741 agtgagcatc gaacaatcca ggcgtaatca cttttccgct gtatggcaga gcgtctctat
     1801 ctccttcagc tgcagaaacg ctcttgatct ttccatcttc cacaatcagc gttcccactt
     1861 cgaagtcttc agctgctgca gtaagaattc ttgcaccaga aaacactttc accaaaaaaa
     1921 cctcctaagc tgttaaaaca agacttttca tttctgattt aagacttctg tttacgccct
     1981 gttaaccctg cttcaccaca aaatatactt gacctgatct tgccactgca gggcttagag
     2041 caggctttct tgacacatcc acgatgttag ataaaataaa agcagaaagc aaggttttaa
     2101 acctatctgc ttatactctg tttacaagga ggagcaggaa tgcctaaacg cacaagtttc
     2161 ctttcattca tatgcctttt gttctttgct gtaggtgctg tggctgctcc tatttctatc
     2221 tggtctccgc tgcacgttga gtggaacaat atcatccaac agctgtcgga tttagagttc
     2281 actccgaaat tcgggattcg ggtaaaggtc cattccattc ccagaagcgg ctatgagcag
     2341 atataccttc tgtctgcagc cagtggcaat gttccggaga tcggcattac aagcctcatc
     2401 ggcgccaccg acctaggggt taggggtgta accgtagatc tttcccagta cggtgaagag
     2461 tacttagaga tcaaacagca gatctatcca gggtcctcca gggctctttc ctacaacg
//
LOCUS       DAOSDA010000272         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_27044_length_2513_cov_3.711554, whole genome shotgun sequence.
ACCESSION   DAOSDA010000272 DAOSDA010000000
VERSION     DAOSDA010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27044_length_2513_cov_3.711554"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="PLW82_06585"
     CDS             <1..1246
                     /locus_tag="PLW82_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ46977.1"
                     /translation="VDYVTFGNFFTRGDIARLLDQFADIGIHILFPEVYAYGTTIYPS
                     EVAKIQDQYLYLWEDGDILEVFIEEAHSRGMEVHPLVRVFSSGYKTPGYLINQHPEWL
                     EVTRDGTRGTSNSWFISPVIPEFRRFLEAVMTELVTNYDIDGLHLDYIRYEEGDFGYS
                     SHSRDLYKKLFFRDPIEFVLGSAEDEAYKDFRRAHVDAFVRWCYQNLKEIKPDLLISA
                     AVGSPHSWGYKSLFQDWVFWSTNGYIDFVTPMQYRDTTPKLQTAIREDKSANAASVPL
                     FSGLGLYLFINSATELESQIAEVQKQNLPGVALFSEANLSVYSYTRIKNGIFREKAVP
                     VHRDPEYAIKVYIDDLCERILKNGRYLGLEEEVLQSYVEQIQESGGSVGVALGKYPRL
                     EQSINTRLEYVKELHKYYKIDY"
     gene            1319..2224
                     /gene="cysK"
                     /locus_tag="PLW82_06590"
     CDS             1319..2224
                     /gene="cysK"
                     /locus_tag="PLW82_06590"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009985206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOJ46978.1"
                     /translation="MKVYQNILELIGNTPLLQIADNKAKVWAKLESFNAGGSVKDRIA
                     KAMIEEAERQGVLKPGTVIVEPSSGNTGVGLAMVAAAKGYKLIITMPETMSIERRRLL
                     SAYGATVVLTDGALGMTGAIQKAEELREQYDGFMPQQFKNSANPDIHEKTTGVEIWND
                     TDGQVDVLVSGVGTGGTITGAGRYLKSKKPTVKLIAVEPTKSPVLSGGQKGPHKIQGI
                     GAGFVPDVLDLSIVDEIVQVADEDAMATAVMLARTNGLLVGISSGAAAYAALQLSEKD
                     EYAGKNIVVVLPDTGERYLSTGIFD"
BASE COUNT          703 a          544 c          643 g          623 t
ORIGIN      
        1 ggtagactat gtaacttttg ggaacttctt cactcggggt gatattgcaa ggctcctcga
       61 tcagtttgct gatataggca tccacattct gtttccggag gtttacgcct atggcactac
      121 catctacccc agtgaagtgg caaagatcca ggaccagtac ctctatcttt gggaagatgg
      181 cgatatctta gaggttttta tcgaagaagc ccatagccgt ggcatggaag ttcatccgct
      241 ggttagggtg ttttcgtcag gatacaaaac gcccggctac ttaattaacc aacatcctga
      301 atggctagag gtcacaagag atggaacccg gggtacatcc aactcctggt ttatttctcc
      361 agtgataccc gagtttcgtc gattccttga agcggtaatg accgaactgg taacaaacta
      421 cgatattgac ggattgcatc tggactacat ccgctatgaa gaaggtgact tcggttatag
      481 cagtcattcc cgtgacttgt acaagaagct gtttttccgg gatcctattg agtttgttct
      541 cggaagcgca gaagacgaag cgtacaaaga cttccggaga gctcacgttg acgcttttgt
      601 caggtggtgt taccaaaacc taaaggagat taagcccgat ctcctgattt ctgcagctgt
      661 aggttcgcca cactcttggg gatataagtc tcttttccaa gactgggtat tttggtctac
      721 caatggttac atcgattttg tgacgccaat gcagtatagg gacactacac cgaaactcca
      781 aaccgcaatt agagaagaca agagtgcaaa cgccgcatca gtgccgcttt tcagcggttt
      841 gggcttgtac cttttcatca acagtgccac tgagctagaa agccagatcg cagaggtgca
      901 aaagcagaat ctcccggggg tagccctgtt ttccgaagct aatctcagtg tttactctta
      961 cacgagaata aaaaacggca ttttccgtga aaaggccgtt ccggtgcacc gcgatccgga
     1021 atacgctatc aaagtgtata tcgatgacct gtgtgaaaga atcctcaaaa acggtcgtta
     1081 tttaggtctg gaagaggagg tactgcagag ttatgtagag cagatccagg aaagcggagg
     1141 atcggtagga gttgcgttag gcaagtaccc aagactggaa caatcgatca ataccaggct
     1201 ggagtatgta aaagaactac acaagtacta caaaattgac tattgacaaa gggaaaatac
     1261 aggtataaag ttaaattacc taattccgat aggaatactt gtaattagga ggagcgttat
     1321 gaaagtttac caaaacatcc tcgaactgat tggaaataca ccccttttgc agatagccga
     1381 taacaaggcg aaagtttggg caaaacttga gtctttcaat gctggtggtt cggtgaaaga
     1441 tcgtatagcc aaagccatga ttgaagaggc tgaacggcaa ggtgttctca aaccaggtac
     1501 agtgattgtt gagccatcca gtggcaacac aggtgttggc cttgcgatgg tggctgctgc
     1561 taaaggctac aagctcatta ttaccatgcc tgagacgatg agcatagaaa gaagaagact
     1621 tttgagcgcg tatggcgcaa ctgttgtcct taccgatgga gccctgggaa tgacaggtgc
     1681 tatccaaaaa gcagaagagc tgagagaaca gtacgacggc tttatgcctc agcagttcaa
     1741 aaactcagca aacccggata ttcacgagaa gaccactggg gtagagatct ggaacgatac
     1801 agatggtcag gttgacgtcc tggtgtctgg agtcggtaca gggggaacca tcaccggtgc
     1861 tggcagatat ttgaagtcga aaaaacccac tgtgaagctg attgccgtag agccaaccaa
     1921 atcacctgtt ctctccggcg gacagaaagg tccccataag atacagggta tcggagcagg
     1981 tttcgttccg gatgttctag atttaagcat tgtcgacgaa atcgtccaag tggcagacga
     2041 agacgccatg gctacagcag ttatgctggc acggacgaac ggccttttag tgggcatttc
     2101 cagtggtgca gcagcatatg ccgcattaca gctcagcgaa aaagacgagt atgctgggaa
     2161 aaacatcgta gtggtactcc ccgacactgg agaaaggtat ttatccacag gaatctttga
     2221 ctgattcata gaaactccga agttcaatag gggctttagg gtcagacagg agttctgtcc
     2281 agagagattt atctccagtg cgtttttgca gacagaaaca gctataagcg cgtattaggt
     2341 ggctagggtc ttccctggcc acttttttta ttacctcggg atcgaggtgt tcgttgctga
     2401 aagcaagggc aatgagggca tttcgtctaa tggtgttgat gccacgccaa cctgcactgc
     2461 tttctttgat cagccttcta agtccggcat ctggcactac aaggctcata aag
//
LOCUS       DAOSDA010000273         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_27110_length_2509_cov_2.850041, whole genome shotgun sequence.
ACCESSION   DAOSDA010000273 DAOSDA010000000
VERSION     DAOSDA010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27110_length_2509_cov_2.850041"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1009)
                     /gene="folD"
                     /locus_tag="PLW82_06595"
     CDS             complement(164..1009)
                     /gene="folD"
                     /locus_tag="PLW82_06595"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013488314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOJ46979.1"
                     /translation="MIIDGKALAKEKRAEIAKQVEALKEKGVTPGLAVVLVGEDPASQ
                     IYVRNKHKACEEVGIYSRKITLPEETTEEEILKIIDELNNDPEIDGILVQLPLPKHID
                     PDKVILSISPDKDVDGFHPVNAGRLLTGQDGFFPCTPLAVMELIKSAGVDVSGKEAVV
                     VGRSNIVGKPVSMLLLRENATVTICHSRTKNLADVCRRADILVAAVGRPEMITADYVK
                     EGAVVIDVGINRVGEKKVVGDVAFDEVKDKAAYITPVPGGVGPMTITMLLYNTLLSAQ
                     KRVSK"
     gene            complement(1022..>2509)
                     /locus_tag="PLW82_06600"
     CDS             complement(1022..>2509)
                     /locus_tag="PLW82_06600"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOJ46980.1"
                     /translation="AITPTPAGEGKTCTSIGLTQGLGAIGKQSMVCLREPALGPTFGI
                     KGGAAGGGYSQVVPMEDINLHFTGDIHAITSAHNLLADLIDNHIYFGNSLNIDPAEVT
                     WPLVMDMNYRHLRNIVLGLGGKVDGRPRQTGFDITVASEIMAILCLAKDMDDLKERLS
                     RMIIGYTYDGEPVTAGQIGGVGAMAILLKDAIKPNLVQTLEGQPAFIHGGPFANIAHG
                     NNSIIATKMALTLSDYVVTEGGFGSDLGAEKFMDIVAGYSGLKPNAVVVVATARALKM
                     HGRVPREKVSEENIEALKLGLVNLDKHVSNMQLYGVPVVVAINRFPSDTERELALIKD
                     HCKELGVRAAISEVVAKGGEGGRELAQAVVEATEEPSSFKPLYDWNLPIKEKMEIIAT
                     KIYGASGVSYSKKAEDQIARYTKLGFDKLPLCVAKTQSSLSDDPTKKGAPTGWTLEVR
                     EIRISRGAGFLVPLTGTMMTMPGLPKEPAALNMDITNDGTITGLF"
BASE COUNT          553 a          700 c          564 g          692 t
ORIGIN      
        1 aatccaccaa gcaaatccct aggttttcgc ccgcaaaacc gcaaaatggc cgttttcacc
       61 gctttatgga gcccaatcct ttggattacg tccatttgct ccccttgtgg atttttttga
      121 cgtttaaaac cccaacaaaa aacggagctt tgcgctccgt tcctcacttc gaaaccctct
      181 tttgtgcact taagagagta ttgtacagca gcatggttat ggtcatgggc ccaacccctc
      241 ctggaacggg agtgatgtaa gcggctttgt ctttcacttc gtcaaaagca acatctccca
      301 caaccttctt ctcaccaacg cggttgatgc caacgtcaat caccactgcg ccttctttga
      361 cgtaatctgc agtgatcatc tccggccttc ccacagctgc aacaagaata tcagctctcc
      421 tacagacatc ggcgagattc ttagttctgg aatggcagat agtgaccgta gcgttttctc
      481 tgagaaggag catagaaacc ggtttcccca ctatattgct tctccctaca accaccgctt
      541 ctttcccgct aacatccact cctgcgcttt tgatcagctc cataactgcc agcggtgtac
      601 agggaaaaaa cccatcctgg ccggtaagaa ggcgtcctgc attaaccgga tggaagccat
      661 ccacatcctt gtctggggag atgcttagga tcaccttgtc cggatcaatg tgttttggga
      721 ggggaagttg gacaagtatt ccatcgatct cgggatcgtt gttcagctca tcgatgattt
      781 tgaggatttc ttcttctgtg gtttcctcag gaagagtgat ctttctcgag tatataccaa
      841 cctcttcaca agccttgtgt ttatttctca cgtagatctg tgatgcagga tcttcaccca
      901 ccaaaaccac cgcaaggcct ggggtaaccc ctttctcttt caaagcctcc acctgttttg
      961 ctatctccgc tctcttttct ttagccaaag ctttaccatc tataatcaaa agaagccctc
     1021 cttaaaacag acctgtgatc gtgccgtcgt ttgtaatatc catatttaag gctgcaggct
     1081 cttttggcag ccccggcatg gtcatcatgg tgccggtgag aggaaccaaa aaccctgctc
     1141 ctctagagat tctgatttct ctaacctcta acgtccatcc agttggagcg ccttttttcg
     1201 tcggatcgtc gctcaaagac gactgggtct tggcaacgca gagaggcagc ttgtcaaagc
     1261 caagcttggt atagcgggca atctggtctt ctgccttctt gctgtagctt acgccggatg
     1321 caccgtagat cttcgttgca atgatttcca tcttctcttt gatggggagg ttccagtcgt
     1381 atagaggttt gaagcttgac ggctcttctg tagcttccac aacggcttga gccagctccc
     1441 gtccaccttc tccgccttta gccacaactt ctgagatcgc ggcgcgcacg cccagctctt
     1501 tgcagtgatc cttaatcaag gcaagttctc tctctgtatc gctcgggaat ctgttgattg
     1561 ccaccaccac cggaactccg tagagctgca tgttggaaac atgcttatca agattcacaa
     1621 gacctaactt cagcgcttcg atattctctt cgcttacttt ctctcttggc actctaccgt
     1681 gcatcttgag cgctcgtgcc gtagccacca caactacagc attgggtttg agtccgctgt
     1741 agcctgctac aatatccatg aacttctccg cgcctaaatc cgaaccaaaa ccaccctctg
     1801 ttactacata atccgagagg gtcagtgcca tcttggtggc gatgatgctg ttgttcccgt
     1861 gagcgatatt ggcaaaagga cctccatgga tgaaggcagg ctgcccttca agggtctgca
     1921 ccagatttgg cttaatggca tctttgagta gaatggccat ggctcccact ccgccgatct
     1981 gtcctgcggt aacaggctct ccgtcatagg tgtagccgat gatcattctg ctcagtctct
     2041 ctttaagatc gtccatgtct ttagcaaggc acagaatggc catgatctcc gatgccaccg
     2101 tgatatcgaa tcccgtttgt ctcggtctgc cgtcaacttt ccctccaaga cccagtacga
     2161 tgtttctcag atgccggtag ttcatgtcca taaccaacgg ccaggtgact tctgcaggat
     2221 cgatgttgag actgttgccg aagtaaatgt gattgtctat gagatctgca aggaggttat
     2281 gcgccgaggt aatagcgtga atatcgcctg taaagtgcag gttaatatct tccatgggca
     2341 ctacctgcga gtagccgcct ccggcggctc cgcctttgat accaaaggtt gggccaaggg
     2401 caggttccct gaggcagacc atgctctgct tgccgatcgc accaaggcct tgagtaagcc
     2461 cgatcgacgt gcaggtcttc ccttcacctg caggtgtagg tgtaatggc
//
LOCUS       DAOSDA010000274         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_27166_length_2505_cov_4.145714, whole genome shotgun sequence.
ACCESSION   DAOSDA010000274 DAOSDA010000000
VERSION     DAOSDA010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27166_length_2505_cov_4.145714"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLW82_06605"
     CDS             complement(<1..409)
                     /locus_tag="PLW82_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46981.1"
                     /translation="MKYRKLAIVLIVLVIVGGIIGFVRGVNAELPPDEDLLTLLQVAA
                     LVKTQYIEDVSFISLLDNYSQTGTINGMLEETLDDKYSYFMTPYEYNEMRIQTQGVYG
                     GIGVIIGLAEGDKVIVVDPFPGSPGYEAGLRPGD"
     gene            complement(509..1681)
                     /locus_tag="PLW82_06610"
     CDS             complement(509..1681)
                     /locus_tag="PLW82_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOJ46982.1"
                     /translation="MAKRIAILLLIVAVAASALAQTSIKQTAEQFENLKKEMEKVRAS
                     LEASSQKQQQILTNLNDLDKTLEKLDAEKAQINAAIQKVSDELATLEKEMQKRQQELA
                     ESEKKLEALREKASSSFRNMQKINAQGWWSFLFSADSISEFWVRSHQIRQMIKGDMET
                     INELNVVYKEHQEIIKDLQAKTAEVQKKKKELENTQKQLQANEAQIKKNIEEKKQALQ
                     KIEQERAEYDRALREMEKASQELGDAIRRLQEKTLEEDGVKKTISMIWPLKGRISSDY
                     GWRFHPILNENRMHTGIDIAAAQGTDIKAAADGTVILAGWVSGYGLTTVISHGNNITT
                     LYGHASKLLTQAGDKVKQGQVIALVGSTGMSSGPHLHFEIRSNGDPVNPWGWLPVE"
     gene            complement(1666..>2505)
                     /locus_tag="PLW82_06615"
     CDS             complement(1666..>2505)
                     /locus_tag="PLW82_06615"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOJ46983.1"
                     /translation="DKGLSNLWRHKGSTVAAFVTVTIALVVLGFFALTWLNVLTLTSS
                     LLNNLEIRAFLVKNQRINQIETLPGITQFTFITPEMAMKEMTDSYPLYGEYFQGILTD
                     NPLQASYALKAEDSQLIPEIVEKLKAMPEVDEVIYGGQATDTLLNLQRFIGGFGFLLF
                     FILTTAILLVVANTLRLSFAIRKPEILLEKLLGASPLYIVGPFLWEGTVLGFLSSVAA
                     LLIILTSYNSFASWAAQAMPYLPFRTLESVRYGLITAGLILGTTMGLIGSIWGVRRYW
                     PKE"
BASE COUNT          613 a          630 c          547 g          715 t
ORIGIN      
        1 catcaccagg tctaagtcct gcttcatagc cgggagagcc gggaaaagga tcgacgacaa
       61 ttactttatc tccctcagcc aggccgataa tcacgccgat gcccccgtag actccttgag
      121 tttgaatcct catctcattg tactcgtagg gagtcatgaa gtagctgtac ttgtcatcga
      181 gggtctcctc aagcataccg ttaatcgttc ctgtctggct gtaattgtcc agtagactga
      241 tgaagctcac atcctcaatg tactgcgttt ttaccaaagc agccacttgt agaagtgtaa
      301 gcagatcttc atctggcgga agctctgcat tgactccgcg gacaaagcca atgatgcctc
      361 cgacgatgac caggactatt aaaacaatcg ctagtttgcg gtatttcatt gtaagcactc
      421 tcccatctag ttcctgcaag agaccaaagg tgcagattgt ttctaccctt aattgtatta
      481 ggaactagct tgtttcaatc ctagcaaatt actcaacggg aagccatccc catggattga
      541 ctggatcgcc gttggagcgg atttcgaagt gcaggtgagg accagagctc ataccggtgg
      601 aacccaccag agcaatgacc tgaccctgct tcaccttatc tccagcttgg gtcaggagct
      661 tggaagcatg accgtaaagg gtagtaatat tattaccatg agaaatcacc gtagtcaacc
      721 cgtaccctga aacccagccg gcaagaataa ccgtgccgtc tgctgcagct ttaatgtctg
      781 ttccctgagc agcagcaata tctatccctg tgtgcatgcg gttttcattg agaatcggat
      841 gaaatctcca accgtaatct gaggagatgc gtcccttcaa aggccagatc atactgatgg
      901 tcttcttcac gccatcctct tccagggttt tctcctgcag gcggcggata gcatcgccaa
      961 gttcttggga ggctttttcc atttctcgaa gagctctgtc atactctgct ctttcctgct
     1021 cgatcttctg cagggcttgc ttcttctctt cgatgttctt tttaatctgc gcttcgtttg
     1081 cttggagctg tttctgagtg ttttccagct cctttttctt cttctgaact tcggcagttt
     1141 tggcctgcag gtctttgatg atctcctgat gctctttata cactacgttc aactcattga
     1201 tagtctccat gtcacctttg atcatctgcc taatctggtg agatcgcacc caaaactccg
     1261 aaatgctatc tgcagaaaac aggaaggacc accagccctg ggcattgatc ttttgcatgt
     1321 tcctaaagct tgagctggcc ttttcccgca atgcttccaa tttcttttcg ctttctgcga
     1381 gctcttgctg cctcttttgc atttcttttt ctagagtcgc cagttcgtca gagacttttt
     1441 ggatggcagc gttgatttgt gccttttcgg catctagttt ttccagagtt ttgtccaaat
     1501 cgttgagatt cgtcaggatc tgctgctgct tttggctgct agcctctaaa gacgctcgaa
     1561 ccttctccat ctcttttttg aggttttcaa actgttcggc agtttgctta atagaagtct
     1621 gggccaaagc agaagcagca acagctacaa tcaacaacag tatagctatt cttttggcca
     1681 atatcttcta accccccata tgctgccgat taaacccatg gtagtaccta aaataagacc
     1741 agcggttatc agaccgtatc ggaccgactc taaggttcta aacggaagat agggcatggc
     1801 ttgagctgcc caggacgcaa atgagttgta gctggtgagg ataatcaaaa gggcagctac
     1861 agaactcaaa aatcccagca ccgtcccttc ccataggaat gggccaacga tgtagagagg
     1921 gctggctcct aagagcttct ccagcaggat ctccggtttt cttatcgcaa agctcaacct
     1981 gagggtgtta gccacaacaa gaagaattgc agtagttaaa atgaaaaaca gtaagaagcc
     2041 aaacccgccg ataaacctct gcagattaag cagagtgtct gtagcctgac ctccgtagat
     2101 gacctcatct acctcgggca tagctttgag cttctccacg atctccggta taagctggga
     2161 atcctctgct ttcagcgcat aactcgcctg tagaggatta tctgtcaaaa tcccttggaa
     2221 gtactcgccg tacagaggat agctatcggt catctctttc atggccatct cgggagtaat
     2281 gaaggtgaac tgagtaattc ccggtagtgt ttcaatctga tttattcttt gatttttgac
     2341 taaaaatgct ctgatttcca aattgtttaa gagactgcta gttaaagtca acacgttcaa
     2401 ccacgtaagt gcaaagaagc caaggacaac caatgctatg gttactgtca caaacgcggc
     2461 aacagtggaa cccttatgtc gccaaaggtt acttaggcct ttgtc
//
LOCUS       DAOSDA010000275         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_27552_length_2480_cov_2.932371, whole genome shotgun sequence.
ACCESSION   DAOSDA010000275 DAOSDA010000000
VERSION     DAOSDA010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27552_length_2480_cov_2.932371"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..662)
                     /gene="speD"
                     /locus_tag="PLW82_06620"
     CDS             complement(273..662)
                     /gene="speD"
                     /locus_tag="PLW82_06620"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOJ46984.1"
                     /translation="MPQALGRHIICEAYGCDKEILNDVRRVEKIMVAAALACGAEVRE
                     VAFHRFSPQGVSGVVVISESHLAIHTWPELGYAAIDVFTCGDTVDPWVACNFLIEKFD
                     AQNVQSFEVQRGLVPPVLLKIDDERVS"
     gene            complement(753..2000)
                     /locus_tag="PLW82_06625"
     CDS             complement(753..2000)
                     /locus_tag="PLW82_06625"
                     /inference="COORDINATES: protein motif:HMM:NF018540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HOJ46985.1"
                     /translation="MYKILSIPDSLLKEVEDAENQLQAQFDNLERICEYWSIRILDAF
                     RKAQVSEYSFFSSSGYGYNDEGRSAVEEVYSRVFGTESALVRPQMVSGTHAITLGFRG
                     ILRPGDRVLIATGLPYDTMQTVLGISGNSPSALKNFGISFDVLPLQEDDSIDLEGLQD
                     ELKKPTKMVYFQRSRGYSDRHTFSDIELEEAMRLVRKQSDAIIYVDNCYGEFVSWTEP
                     TFYGADLMAGSLIKNPGGGLAQSGGYLVGRADLVELAAEALTSPGLGSKLGATFNQTK
                     LVLQGLYQAPLMVLQAMKSATLFSKVLQSRGYWVSPRPEEPRFDTVQLIGFHTRDELI
                     QFCRAIQHASPVDSYVTPEPGPMPGYEHEVIMAAGTFVQGATSELSADAPLREPYRAY
                     LQGSLNYFQSKLALLEFLTKLLD"
     gene            complement(2080..>2480)
                     /locus_tag="PLW82_06630"
     CDS             complement(2080..>2480)
                     /locus_tag="PLW82_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage V sporulation protein K"
                     /protein_id="HOJ46986.1"
                     /translation="DFGREALDTLVKAMEDHRQDLVVIAAGYPDEMEMFIRLNPGLVS
                     RMPISIHFPDYSAEELMQIALLILAKSDYRLSPKAVEKLREKLQGLKGNGRDVRNILE
                     QAMRRQAVRLESLSHVKRDDLMTIKPEDLE"
BASE COUNT          628 a          668 c          538 g          646 t
ORIGIN      
        1 atccgtaacg atctccgtac ctatttcttc cagttcagcc cggagaattg tctgaacatc
       61 accttcaaat ccggtgactc ccggagcatt gcaaagtttt tccagaaacg agagcccaac
      121 attcataagt agcatttatc cccctatgat cacttatttg tcgtttatat tctcctttaa
      181 cctctctatt gcctgcttaa taagacaaaa acaaaaaaag tctatcccgt ttttgcgaga
      241 aacggagata gacttctttt gatcggtctc agtcaagata ctctctcatc atctatcttt
      301 aacagtacag gtggaaccaa gccccgctgc acttcgaaag actggacgtt ttgagcatca
      361 aacttctcaa tcagaaaatt gcatgcaacc caggggtcca cagtgtcgcc acaagtaaac
      421 acatcaatgg cagcataacc aagttccggc caggtgtgaa tggccaaatg ggattcggaa
      481 atcaccacaa caccgctaac tccctgggga ctaaagcggt gaaaggcaac ctctcggacc
      541 tctgcaccgc aagcaagtgc tgcagccacc ataatctttt ccactcggcg aacatcgtta
      601 agaatctctt tgtcgcaccc ataagcctca caaataatgt gtctacccaa agcctggggc
      661 aacttatctc cctccttttg tgtttctatt tttcagatag aaaccagaat ttgtattaaa
      721 atgtttagtt aagtatactg acgattcctc tttcagtcaa gcaacttagt caaaaactcc
      781 aacaaagcaa gtttagattg gaaatagttc aacgatcctt gcaaatatgc ccgatacggt
      841 tctcttaaag gagcgtctgc gctcaactcc gaagtagcac cctgcacaaa cgtaccggca
      901 gccatgatta cttcatgttc gtaaccgggc atcggccctg gctctggagt aacgtagcta
      961 tccaccggag atgcgtgctg gattgccctg cagaactgaa ttagctcgtc tctggtatga
     1021 aagccgatca gctgaaccgt atcgaatctt ggctcttcag gccggggaga aacccagtat
     1081 ccccgtgact gcagaacctt actgaacaag gttgccgact tcattgcctg aagcaccatc
     1141 aacggtgcct ggtacaatcc ctgcagaact agcttggtct ggttgaaggt tgcccctagc
     1201 ttggatccaa gacctggcga agttaaagct tcagctgcca gctctaccaa gtcagctcgt
     1261 ccaacaagat aacctcccga ttgagcgagc cctcctccag ggtttttgat cagagagccg
     1321 gccataagat ctgcaccgta gaaggtcggt tccgtccaag agacaaactc cccgtaacag
     1381 ttatccacat agatgatggc atcgctttgc ttgcgaacta gcctcatagc ttcttcaagc
     1441 tctatgtcgc tgaacgtgtg cctgtcgctg tagcccctag accgctggaa gtaaaccatc
     1501 ttggtgggct ttttgagttc gtcctgcaaa ccttcgagat cgatactgtc gtcttcctga
     1561 aggggcaaaa cgtcaaagct aatgccgaaa ttctttaatg ccgaaggaga atttccggag
     1621 attccgagca cggtctgcat ggtatcatac ggaagtcccg tggcaatcag gacccggtct
     1681 ccaggccgga gaatccccct aaagcccaga gtgatggcgt gggtgcctga aaccatctgc
     1741 ggccttacca gggcgctttc ggtaccgaat accctgctgt agacctcttc aacggcagat
     1801 cggccttcat cgttgtagcc gtagccgctg ctcgaaaaga aactgtattc actgacctga
     1861 gcttttctaa aagcgtcaag gatgcgtatg ctccagtatt cgcagatcct ctccaagttg
     1921 tcgaactgcg cttgcagctg attttctgcg tcttctactt ctttgagcaa tgagtcgggt
     1981 atgcttaaga tcttgtacat gggtgtcctc ctgcaatcgt taacattcat tactaactct
     2041 atctttaacc ttctcggctg gaataaaaac gaaaagctct tattccaggt cctccggttt
     2101 gatggtcatg agatcgtctc tttttacatg gctaaggctt tctaacctta ccgcctgcct
     2161 gcgcatggcc tgttccaaga tgttccgcac gtctcggcca ttgcctttta agccctgaag
     2221 cttctcccga agtttttcca cagcctttgg agagaggcgg taatcggact tggccagaat
     2281 caagagggct atttgcataa gttcttcggc ggagtaatcg ggaaagtgga tgctaatggg
     2341 cattctggaa acaagccccg ggttcaacct aataaacatc tccatctcat cggggtaccc
     2401 cgcagcaatc accaccaaat cttggcggtg atcctccatg gccttcacca gagtatctag
     2461 tgcttctctg ccgaaatcct
//
LOCUS       DAOSDA010000276         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_27641_length_2474_cov_3.269119, whole genome shotgun sequence.
ACCESSION   DAOSDA010000276 DAOSDA010000000
VERSION     DAOSDA010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27641_length_2474_cov_3.269119"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLW82_06635"
     CDS             <1..205
                     /locus_tag="PLW82_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOJ46987.1"
                     /translation="KLLTLFEEKAKGRSIPVIGIAHVANEKGAQMLKELLQKVHKDAE
                     IFITTMGATIGTYAGPGGIVLAF"
     gene            864..>2474
                     /locus_tag="PLW82_06640"
     CDS             864..>2474
                     /locus_tag="PLW82_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOJ46988.1"
                     /translation="MKQNLFDREEMAKAWEGFVPGNWSEEIDVRDFIQKNYTPYLGDQ
                     SFLEGPTDDTKSLWNKVDEMIQEEVRSKTIKVDLERFSGIDNFAPGYIDKDRELIVGL
                     QTDEPLKRIMNPYGGFRMVKNALEAYGLEMDPDLEQKFNEYRKTHNQGVFDAYTKEMR
                     TVRSVGLLTGLPDAYGRGRIIGDYRRVPLYGVDYLKEMRIRDKENLTGPATDEVIRLR
                     EEFTDQIRALDAMKSMAAKYGCDIGRPAKNAQEAVQWLYLAYLAAVKENNGAAMSFGR
                     NTAFIDIYVERDLQKGLIDEKTAQALVDQLVIKLRLVRHLRTPEYDQLFAGDPTWVTE
                     AIGGIGLDGRPLVTKTAYRFLHTLTNLGTSPEPNMTVLWSKDLPREFKEYCAYMSIKT
                     ASIQYENDDLMRPIYGDDYAIACCVSAMRVGKDMQFFGARANLAKALLYAINGGVDEI
                     KRDKVTGGPITVISGIEKNTEEVLSYDTVVESFKKVMDKLAEIYVNTMNTIHYMHDKY
                     AYEASQLALHDPDVHRFIAFGVAGLSIVA"
BASE COUNT          716 a          609 c          610 g          539 t
ORIGIN      
        1 gaagcttctg acgctcttcg aagaaaaagc caaaggcaga tcgattcctg tgatcggcat
       61 tgcccatgtg gcaaacgaaa aaggagcaca gatgctgaaa gaactgctcc aaaaggttca
      121 caaagacgcc gagatcttca ttaccaccat gggcgcaact atcggtacct acgcaggtcc
      181 tggcggaatt gttttggcct tctaagaaac ttgcagcccc tcgaatacga ccctcgtgtg
      241 ctaataaccc tagcaccacg ggggtttttt taacatctga gccaaagggt gagtcaacga
      301 tatggcttaa agtgcgtact tccgccaaga aaaactggcg tattcggtag cccatgcgga
      361 gtttttgcag ctcgaaactt cggggtactg ataaactcaa acataggagt aaagtactca
      421 agtgattcgc ctaagcgacg atgatgtgaa caggaaaaca atagaaaaac gcagttttgc
      481 ctattcacaa actggacgaa atcatttata actttgataa aggtggagcg cttggaggcg
      541 aggagccttc ccgttacata tccgatcttg gacgagcctt ttccacgtct gagggaagga
      601 ccaaacaacc actcggaggg acgcatagtc gttccttccc atcaaacaga ctccaaacta
      661 gaagacgttc tttcgatacc gtaaagcccg acagacggca gtttctgcct tgtttcgggg
      721 attttttcgt taatgtgtgc aaaaagtgat tcaagtcacg gcaagttttt ttgagagata
      781 ctataatgaa cacgcaatca tggggtaagt acatgcccag tactgctcat ataactagtt
      841 tcttttggaa aggtgatatg tatatgaagc aaaacttgtt cgacagagaa gaaatggcca
      901 aagcttggga aggctttgtt cccgggaact ggtctgaaga gatcgatgtc agagacttta
      961 ttcagaaaaa ctatactcct tacctgggtg atcagagctt tttggaaggt cccacagacg
     1021 ataccaagag tctctggaac aaagtagatg aaatgattca ggaggaagta agaagcaaga
     1081 ccatcaaggt agaccttgaa cggttcagcg gtatcgacaa cttcgctcct ggttacatcg
     1141 acaaggacag agaactgatc gtaggtctcc agaccgatga gcctctgaaa agaatcatga
     1201 acccctacgg cggctttagg atggttaaaa acgctttaga agcctacgga ttggagatgg
     1261 atccagacct ggagcagaag ttcaacgagt acagaaaaac ccacaaccag ggcgtgttcg
     1321 acgcttacac caaggaaatg cgcactgtgc gttcggtagg gctccttaca ggtcttcccg
     1381 atgcctacgg cagaggaaga atcatcggcg actaccgcag ggtacccctc tacggcgtcg
     1441 attaccttaa agagatgcgc atcagagaca aagaaaacct gacaggtcct gccaccgatg
     1501 aagttatcag gctccgcgag gagttcaccg accagattag ggcacttgac gcgatgaagt
     1561 ccatggctgc taaatacggc tgcgacatcg gcagacctgc aaaaaatgcc caagaagccg
     1621 ttcagtggct gtaccttgcc taccttgcag cagtcaagga aaacaacggt gcagctatga
     1681 gctttggcag aaacaccgca tttatcgaca tctatgttga aagagatctg cagaaaggtc
     1741 tgatcgacga gaagaccgca caagccctcg tagaccagct ggtgatcaag ctgcgcttgg
     1801 tacgccactt aagaacgccc gagtacgacc agctctttgc tggagatcca acctgggtga
     1861 ccgaggccat cggcggtata ggtctcgacg gacgtcctct ggtaacaaag accgcctaca
     1921 ggttcctgca caccctcacc aacctgggca catctcccga gccgaatatg acggttctct
     1981 ggtccaaaga tctgcctaga gagtttaagg aatactgcgc ctacatgagc atcaagaccg
     2041 catccatcca gtacgaaaac gatgatctca tgcgcccgat ctacggcgac gactacgcta
     2101 tcgcctgctg cgtctctgcc atgagagtag ggaaagacat gcagttcttt ggcgccagag
     2161 caaaccttgc caaagcactg ctctacgcta tcaacggcgg agtagatgag atcaagcgcg
     2221 acaaggtaac cggcggtccc atcaccgtaa tttctggcat tgaaaagaac acagaagagg
     2281 ttttgagcta cgacactgta gtggaaagct tcaagaaggt catggataaa cttgctgaga
     2341 tctatgtaaa caccatgaac accatccact acatgcacga caagtacgcc tatgaagcaa
     2401 gccagctagc tctccacgat cccgacgtgc acagattcat cgcttttggc gttgcaggtc
     2461 tttccatcgt tgcc
//
LOCUS       DAOSDA010000277         2469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_27714_length_2469_cov_2.951533, whole genome shotgun sequence.
ACCESSION   DAOSDA010000277 DAOSDA010000000
VERSION     DAOSDA010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27714_length_2469_cov_2.951533"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLW82_06645"
     CDS             <1..734
                     /locus_tag="PLW82_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOJ46989.1"
                     /translation="GDAILVCTPNGSTLLIDAGPRVAGSTVVEYLKKLGIHEIDVVVA
                     THPHEDHIGGFPAVFESFSVDRVYFTDHEHTTKTYERFLDAVNASGAYRVRAVAGKTI
                     DIDPEVQIEILSPLDKRYRDLNHSSVVLRLTYGKTAFLFTGDMEVLNENELLAEKVAV
                     GADVLKVAHHGSSTSSSMVFLEQVSPTVAVIMCGSGNSYGHPHREVLDRLESLHVEVL
                     RTDLLGTVVIESNGMELTFRSKSGS"
     gene            791..1492
                     /locus_tag="PLW82_06650"
     CDS             791..1492
                     /locus_tag="PLW82_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46990.1"
                     /translation="METYTIGELNESSLHKALKARYMEPELSAEVLFEGYVVDLLGKE
                     EIIEIQTANFSGLKAKLRKLLDERDVRLVYPLPKDKWIVKLSSEGEVLSRRLSPKHCH
                     FDDAFHELVYIAELLSRPNLKVEILLIQEEEIRVDDGRGSWRRKGVSIRDHRLLDVLS
                     SVTLESPRDYLCFLPDCLGETFTNQNLAEAGRLTVSLARKITYTLRKMGLFEVRKTQD
                     RKHYYAKASGGDELE"
     gene            1485..>2469
                     /locus_tag="PLW82_06655"
     CDS             1485..>2469
                     /locus_tag="PLW82_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ46991.1"
                     /translation="MSRKLGLLAVLLVVFVFAADVFALTRAEETLKIVERDATRLKNL
                     VANLDEYLDRPEETESQLQLLDSIIEEAKYYMEIGDEFEAELKLALAKIVIGRAYAAA
                     SRSPLAETRIISIDASTLSFRAKQGTLPQLVQQIKDTGFNTVFVEVLRDDGYVVYPSK
                     YMILAPDLEGLDPLGELVRLCNENGIDVFPWQKVFFAAADGTPGPILTEHPEWAAVDR
                     FGKPFEASGLAWFSPAHPEARELIFNYILEMWEMYDFAGCQLDYVRNATNPLENNDFS
                     YDKATLDLFMTYYGYNPLDLPYPSAYDRTREGYVPGDSGAKWENWQAFREEL"
BASE COUNT          650 a          561 c          665 g          593 t
ORIGIN      
        1 agggagatgc cattttggtg tgcacgccaa acggaagcac tctgctaatc gatgcagggc
       61 ctagagtcgc agggagcacc gttgtagagt accttaaaaa actcgggata cacgagattg
      121 atgtggtggt tgctacgcat ccccacgaag atcacatcgg cgggtttcca gcagtgttcg
      181 agagcttttc ggtggacagg gtctacttca ccgaccacga gcacacgaca aaaacctatg
      241 aacggttctt ggatgcagta aatgcctcag gtgcatatcg ggtccgtgct gttgcaggaa
      301 agaccataga catcgatcca gaggtgcaga tcgagatcct ttcgccactg gacaaaagat
      361 atcgggactt gaaccattct agcgtggtgc tgcggctgac ttacggaaaa accgcttttc
      421 tctttacagg agatatggaa gttctaaatg aaaatgaact tttggcagaa aaagtggcag
      481 ttggagcaga tgttctgaag gtcgcccacc acggaagcag cacgtcatca tccatggttt
      541 ttttggaaca ggtctcaccg actgttgcgg taatcatgtg cggcagcggt aacagctacg
      601 gtcatcctca ccgagaggtg ctggaccggt tggagagttt gcacgttgaa gtcttacgga
      661 ctgatctttt aggtactgtt gtcatcgaaa gtaatggaat ggagcttacc ttccggagca
      721 agtctggatc ttaacactag gtaaaatgcg gcgagaaaag gaccaggtct gaggtcactg
      781 ggggtgccat gtggaaactt acaccatcgg agagctgaac gaaagctctc ttcacaaagc
      841 cctgaaagcc cggtacatgg aaccggagct ttccgctgag gtcttgtttg agggctatgt
      901 tgtggatcta ttgggcaagg aggagatcat cgaaatccag acggctaact tctcaggact
      961 aaaagccaag ctgcgaaagc tcctggatga aagggatgtt cgtctggtgt atcctcttcc
     1021 aaaggacaag tggatcgtca agctcagttc agagggcgag gtgctttccc ggcgcctttc
     1081 acccaaacac tgccactttg acgatgcctt ccacgagctc gtctacattg ccgagctgct
     1141 ttccaggcct aatctcaaag tagagatttt actgatacag gaagaagaga tcagggtaga
     1201 tgatgggagg ggatcctggc gtagaaaagg ggtcagcatc cgggaccatc gccttttgga
     1261 tgttctaagc agcgtgactt tagaaagccc aagagactac ctgtgctttc tgccagactg
     1321 tctaggggag accttcacta accaaaatct tgcagaagca ggtaggttaa cagtgtccct
     1381 ggcgcgaaaa ataacttata ccctgagaaa gatggggctt tttgaagtta ggaaaaccca
     1441 agatagaaaa cactattatg ccaaagcaag tgggggagat gaacttgagt agaaagcttg
     1501 gtttgcttgc tgttttattg gtagttttcg tttttgcagc agatgtgttt gccttaacca
     1561 gagcggagga aaccttgaag attgttgaaa gagatgccac tcgcctgaaa aacttagtgg
     1621 caaacttgga cgagtatctt gaccggccgg aagaaaccga atcccagctg cagcttctcg
     1681 acagcatcat tgaagaagcc aagtattaca tggagatcgg tgatgagttc gaagctgaac
     1741 tcaaacttgc tcttgccaaa atcgtaattg gcagagctta cgctgccgcc agcagaagtc
     1801 ctttggcgga gaccagaatt atctccatcg atgccagcac cctgagtttt cgggcgaagc
     1861 agggaacctt accccaattg gttcagcaga taaaggatac ggggtttaac accgtctttg
     1921 tggaggtctt gagagacgac ggctacgtag tttatccgtc caaatacatg atcctcgctc
     1981 ccgatcttga aggtctggat ccgttaggag aactggttcg cctctgcaac gaaaacggta
     2041 tcgacgtgtt cccctggcag aaggtgtttt ttgccgctgc cgatggaacg cccggtccta
     2101 tcttaaccga gcatccggag tgggcagctg tagatcgctt tggcaaaccc tttgaagcat
     2161 ctggtcttgc atggttcagt ccggcgcacc ctgaagctcg cgaactcatt tttaactata
     2221 tcttggaaat gtgggagatg tacgactttg ctggatgcca gctggactat gtaagaaacg
     2281 ctactaaccc actggaaaac aacgatttca gctacgacaa agctacgttg gacctgttta
     2341 tgacttacta cggctacaat cctctggatc tgccttatcc ttccgcttac gacagaacca
     2401 gggaagggta cgtgccagga gactccggtg ctaaatggga gaactggcag gctttcagag
     2461 aagaactga
//
LOCUS       DAOSDA010000278         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_27726_length_2468_cov_2.899710, whole genome shotgun sequence.
ACCESSION   DAOSDA010000278 DAOSDA010000000
VERSION     DAOSDA010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27726_length_2468_cov_2.899710"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /gene="fliG"
                     /locus_tag="PLW82_06660"
     CDS             complement(<1..642)
                     /gene="fliG"
                     /locus_tag="PLW82_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOJ46992.1"
                     /translation="MQQFRSRRGGLTGKQKAAILMVTLGPELSANVYKHLREEEIEDL
                     TLEIANIKKVEPQLRDEILAEFKEIAKAQEYIATGGIEYARDLLEKAFGPVKAEEIIL
                     RLTSSLQVRPFDFARRADPNQLFNFIQGEHPQTISLIMAYLQPEQAAAILSSLSPEEQ
                     VEVAKRLATMEGTNPEVLSEVESVLEKRLASFINQESTSAGGIQAVVEILNRVD"
     gene            complement(661..2190)
                     /gene="fliF"
                     /locus_tag="PLW82_06665"
     CDS             complement(661..2190)
                     /gene="fliF"
                     /locus_tag="PLW82_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOJ46993.1"
                     /translation="MNQLAQLGNQIKELWQNLDKKIKWLAIILSASIFVTLVAISIWG
                     NTKEFSPLFYDLALVDAGQIMAALEEEKIPYQLKDGGRTILVPSDKVDSLRIKLSASG
                     LLTGGVVGNELFDQTKWGITDFEQQQMFKRALEGELVRSIRTLSGVKDARVHVVLPES
                     SPFLTDTGTATASVVLTLEPYARISADQIKSIANFLAGAVPGLSPQNVTIMDSSWNVL
                     SDPSMFNTQFGQSTAPSNQLELKRQIDRELAVNLQSMLERIFGFGNAIVRVSAEVAFD
                     YKETTAERYEPVTGNRGIVRSEQQSREVQSGTSSSVPIGVAGITSNIPGYTFDTNTQD
                     SSSYERSDTITNYEISKVLEHHVVAPGSIKRLSVAVWLHGDLDESTKEMVRQAVIAAS
                     GLDFGRGDQLTVESMPFFTAETVTEAPLVAAGPELPYIYIVPVAVVLLLVLLFALRGR
                     TRRQREAAVLEETAAVTEAEEEQESVPAPRLTLERELKLVARQKPEEVAKVLQSWLAE
                     D"
     gene            complement(2209..>2468)
                     /gene="fliE"
                     /locus_tag="PLW82_06670"
     CDS             complement(2209..>2468)
                     /gene="fliE"
                     /locus_tag="PLW82_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOJ46994.1"
                     /translation="NTENKSAQKVLTDSFGSFLKDALSSVNQAQLEAEEVSVRVALKD
                     PTVELHDLTIAAQKAAIALDITLAIKDRLIEGYKEIMRMQV"
BASE COUNT          544 a          658 c          585 g          681 t
ORIGIN      
        1 gtctaccctg tttaaaatct caactacagc ttggatgccg ccggcgcttg tggattcctg
       61 gttgatgaag cttgccaggc gcttttccaa caccgactca acttcgctca agacctcagg
      121 gtttgtccct tccatggtag caagacgctt ggccacttcg acctgttctt ccggagaaag
      181 agacgacaga atggctgcag cctgttcagg ctggagatag gccatgatca aggagatcgt
      241 ctggggatgc tccccctgga tgaagttgaa cagctgattg gggtcggctc ttctggcaaa
      301 gtcgaagggc ctgacctgca gcgaagaggt cagcctcagg atgatctcct ctgccttgac
      361 cggaccgaaa gccttctcca gaagatctct ggcatactcg ataccgcctg tggcgatata
      421 ttcctgggct ttagcgatct ctttaaactc cgccaatatc tcgtctctca gctgcggttc
      481 gacttttttg atgttggcga tctccagggt gaggtcttcg atctcttctt ctctcagatg
      541 tttatagaca tttgcagaca gttccggtcc taaagtcacc atgagaatgg ccgctttttg
      601 ctttcctgtt agtcctccgc gtctgcttct aaattgttgc atttcagcca cctctctctc
      661 ctaatcttca gccaaccagg actgaagcac tttagcaact tcttcaggtt tctgtctggc
      721 aacaagcttc agttctcttt caagagtaag ccgtggagca ggaacactct cttgttcttc
      781 ctcggcttca gtaacagctg cagtctcttc aagtacggca gcttctctct ggcgtctggt
      841 gcgaccgcgc agtgcaaaca acagcaccaa caagaggact actgccaccg gcacgatgta
      901 gatgtaaggt aattcgggac cggctgcaac aagtggtgct tcggtaacgg tttcagcagt
      961 gaagaaaggc atgctttcca cggtcagctg atctcctctc ccaaaatcaa gaccgctggc
     1021 tgcaatcaca gcttgcctga ccatctcttt ggtgctttca tccaagtcgc cgtggagcca
     1081 gactgctaca gataaccttt ttatggaacc aggtgctacc acatgatgct ctagcacctt
     1141 ggagatttcg taattggtga tagtatcgga acgctcgtaa gaactgctat cttgggtgtt
     1201 agtgtcgaag gtgtatcctg gtatgttgct ggtgataccg gctacgccga tcggaacaga
     1261 gctgctcgtc cctgattgaa cttctcggct ctgctgctcg cttcgaacaa tgccgcgatt
     1321 tccggttacc ggctcatacc tttcggctgt agtctctttg tagtcgaagg cgacttctgc
     1381 agagactctc acgatggcat tgccaaagcc gaagatccgc tcgagcatgc tctgaaggtt
     1441 aaccgccagt tctctgtcaa tctggcgctt cagctcaagc tgattggaag gcgcggttga
     1501 ttggccgaac tgcgtgttaa acatggacgg atccgacaat acgttccacg aactatccat
     1561 gatggtaaca ttttggggcg acagtcccgg aactgctccc gcaagaaagt tagctatgct
     1621 tttgatctga tctgcactga ttctggcata cggctctaga gtcagcacca cagaagctgt
     1681 tgcagttcca gtgtcggtta aaaagggaga tgattccggc aaaaccacgt gcacccgtgc
     1741 atctttcacg ccgctgaggg tccggatgct tcggactaac tcgccttcga gggctctttt
     1801 gaacatctgc tgttgttcaa aatctgtaat accccatttt gtttgatcaa ataactcatt
     1861 gcctaccacg ccaccggtca agagccccga tgcagacagc ttaatccgca agctgtccac
     1921 cttgtccgat ggcaccaaaa tcgtccttcc accatctttt agctggtagg ggattttctc
     1981 ttcctctaga gcggccatga tctgacctgc atccacaagg gcaaggtcgt aaaacagcgg
     2041 cgaaaactcc ttggtgttgc cccagatcga gatcgccaca agtgtcacaa aaatggatgc
     2101 agacaagatt atggcgagcc atttgatctt tttgtcgagg ttttgccaca gttcttttat
     2161 ttgattcccc agctgcgcca attggttcat tctaacccta ctcctttatt aaacctgcat
     2221 cctcatgatc tctttgtatc cttcaataag tcggtcttta attgccagtg taatgtctag
     2281 cgctatagcc gctttttgtg ctgcaatggt gaggtcgtga agttcaacag taggatcctt
     2341 cagtgccacc cgaacgctca cttcttcagc ctcaagctgc gcctggttaa cgctgcttaa
     2401 tgcatctttt agaaaactgc cgaaggaatc ggttaagacc ttctgggcag acttattttc
     2461 cgtgttga
//
LOCUS       DAOSDA010000279         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_27753_length_2466_cov_2.677727, whole genome shotgun sequence.
ACCESSION   DAOSDA010000279 DAOSDA010000000
VERSION     DAOSDA010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27753_length_2466_cov_2.677727"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLW82_06675"
     CDS             <1..257
                     /locus_tag="PLW82_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46995.1"
                     /translation="PRITQNGRPRTFRVNYAIEGFAQREGDGGPGMFRGVYDISQTLI
                     DVGIDPTQPMEITMMINAVPGPITINHLAVLTDKPSEEAE"
     gene            complement(320..>1960)
                     /locus_tag="PLW82_06680"
     CDS             complement(320..>1960)
                     /locus_tag="PLW82_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ46996.1"
                     /translation="FLCILLAGLCVLMSACVYAGPLDETAILREEMLRKKQEMALLDY
                     NAIEDMIGRAETLFVSTSDQDRIEAYSLIREAKTLLTESRPVETRAVWLDDQALAKIS
                     SPDEMRNLIAWLNDLNVNLILPCVYFAGETIYPSEVVEQATWYRLSFPNDDPLQIIID
                     EARKYGMEVHPWIMVYGLQGNIEPFLDRVYMIDKNAKGQFNDTSHVDYFLSPAHPETV
                     EYLVSLILELAKYDIDGVHLDNIRYKDGFGYGDFAVDLYQRLFGLDPREFTSSSARYN
                     HFREFKAQFVASFVERVRRELFAIDPHLFVSAATAPRTWGKNSLGQDWHNWIDNRSLH
                     FVLPMSYTPDPKEYRELLDFDLKRIGGRTYAFTGMTLYSFTPEVMVEEWKVGQEMPTT
                     GQTLFSLLHIKQSYYEILKKGLFRDPAYPTFRDPQMAACLFLDWLVERFETLGTAVGL
                     KPEEVQSWIGDLSRVKDLIAAFSGPKYGDRDLRTHSSEDNAYWNGVLGEIDALVKKTE
                     SLSGVTQQRIKNDLLQLRSLISPLEYTTRPFELVKITY"
     assembly_gap    1961..1970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2159..>2466
                     /locus_tag="PLW82_06685"
     CDS             2159..>2466
                     /locus_tag="PLW82_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ46997.1"
                     /translation="MLSIKNVYKKYQGADRFAVEDLSLEVKRGEIFGFLGPNGAGKTT
                     TLKMIVGLLLPDRGEILVDGTDVVKDPLGAKKKIGFVPDNPDLYQRLTGIEYLNLIGD
                     V"
BASE COUNT          624 a          650 c          593 g          589 t
ORIGIN      
        1 atcctcgcat cacccaaaac ggcaggcccc ggaccttcag agtcaactac gccatcgaag
       61 gctttgccca gagagaaggt gatggcggtc ctggtatgtt cagaggcgtc tacgacatct
      121 cccaaaccct aatcgacgtt ggcatcgacc ccacacaacc tatggaaatc accatgatga
      181 tcaacgccgt tcccggtccg ataaccatca atcacttggc ggtcctcacc gataaaccga
      241 gtgaagaagc cgaataagct acactcaagc accgttcaaa aagagcctag cgcgattgaa
      301 gtgcgctagg ctctctcttt tagtaagtga tttttaccag ctcaaaaggt ctggtggtgt
      361 actctagagg cgatatcaga ctcctcagct gcaggaggtc gtttttaatt ctctgctggg
      421 ttaccccact caggctctcg gtttttttga ctaaggcgtc gatctcgccg agaaccccat
      481 tccagtaagc attatcttct gagctgtgag tcctaagatc cctgtcgccg tacttcgggc
      541 cgctaaaggc cgcaatcaga tctttgaccc tcgaaagatc gcctatccag ctctgtactt
      601 cctcaggttt taggcctaca gcagtcccaa gagtctcaaa ccgctccact agccagtcca
      661 aaaacaggca ggcagccatt tgcggatccc ggaatgtggg ataggcagga tctcggaaaa
      721 gacccttttt cagaatctcg taataggact gtttgatgtg caggagggaa aacagcgtct
      781 gccctgtagt gggcatctcc tgccctacct tccattcttc taccatcacc tcaggggtga
      841 aggagtacag cgtcatgccg gtgaatgcgt aggtccgccc gccgatacgc ttcaaatcga
      901 agtccaggag ttctctgtac tccttaggat cgggagtata gctcatgggc agcacaaagt
      961 gcaggctcct gttgtcgatc cagttgtgcc aatcctgtcc gagggagttc tttccccaag
     1021 tcctcggtgc tgtggctgca gacacaaaca ggtggggatc gatggcaaag agttctcttc
     1081 tcacccgctc cacaaagctt gcaacaaact gcgctttaaa ctctctgaaa tgattgtacc
     1141 tggcgctact tgaggtaaac tccctaggat caaggccaaa aagcctctga tagagatcta
     1201 cagcaaagtc gccgtagccg aagccgtctt tgtaacggat gttgtcaaga tgaacgccgt
     1261 cgatatcgta cttggcgagt tccaagatga gcgacacgag atattcaacc gtttcaggat
     1321 gagccggaga caggaagtag tccacatggc tggtgtcgtt aaactgtccc ttagcgtttt
     1381 tatcgatcat gtagacgcgg tcaagaaacg gctcgatgtt tccctgcagt ccgtacacca
     1441 taatccacgg atggacctcc atgccgtact tgcgcgcctc gtcgatgatg atctgcagcg
     1501 gatcgtcatt ggggaagctc aaccggtacc atgtcgcctg ttctactact tcagacggat
     1561 agatggtctc tccggcaaaa tacacacacg gcagaatcag gttgacgttt aggtcattca
     1621 gccaggcgat caggtttctc atctcgtctg gcgagctgat cttggccagc gcttggtcgt
     1681 caagccaaac cgctctggtc tccacaggtc gcgattcggt cagaagggtt ttagcttctc
     1741 tgatcagaga gtaagcttct atgcggtcct ggtcggaggt actcacaaaa agcgtttctg
     1801 ccctcccgat catatcctca attgcgttgt agtccaaaag tgccatttcc tgctttttcc
     1861 ggagcatctc ctcacgcagg atagcggttt catcgagcgg ccctgcgtac acgcatgccg
     1921 acattaaaac gcagaggcct gcaagaagaa tgcataaaaa nnnnnnnnnn atacctgtcc
     1981 aatcccaact ctcccaactc cttccacaac ttaagcataa cttcacagta cattattata
     2041 taacagcttg aaaactcctg taagataggg aaaagatgag ttctctactg caggacttat
     2101 tggcttgttt gctaaactaa gtaataagaa aatggctgca ctggaaagga gtaatgtgat
     2161 gctctcgatt aaaaacgttt acaaaaaata ccaaggagcg gacaggtttg cagttgagga
     2221 cttgagtttg gaagtcaaaa gaggcgagat cttcggtttc ctcggtccaa acggtgccgg
     2281 taaaacaacc accctgaaga tgatagtagg gttgcttctg cccgaccgtg gggaaatcct
     2341 cgtggacgga accgatgtag tcaaagaccc cttgggagcc aaaaagaaga tcggctttgt
     2401 cccggacaat cccgatcttt accaaaggct gaccggcatt gagtacctca acctcatagg
     2461 cgacgt
//
LOCUS       DAOSDA010000280         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_27971_length_2453_cov_3.108424, whole genome shotgun sequence.
ACCESSION   DAOSDA010000280 DAOSDA010000000
VERSION     DAOSDA010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27971_length_2453_cov_3.108424"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..909)
                     /locus_tag="PLW82_06690"
     CDS             complement(49..909)
                     /locus_tag="PLW82_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ46998.1"
                     /translation="MYKFVLLALLVLFFMPRAAKASENPLINLALGKTYQVVASWPDA
                     FFADYETKYEDKNGRELTDGQFGTLRYQDAEWRCFYRQGGRTIIVDLGDVQTVNSIKG
                     HFLQNRSAGIFVPRYIDYYLSTDNENFSYMGTVETAVGPWNNRLQKEEFVLDGLNHQA
                     RYVKIQFPIDVYVFMDELEVFGREGIASNSLELQSTPELDLEKRWEKSAVLGPEAIPA
                     KGFMPKGTAQTGGAEDIVLAYYGYAGSGDGTWLSSDFMPYVAYLEAVSPPGRSRTVSK
                     NQSLGVKSSR"
     gene            complement(1006..2283)
                     /locus_tag="PLW82_06695"
     CDS             complement(1006..2283)
                     /locus_tag="PLW82_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ46999.1"
                     /translation="MRKFMLTIALVCVFTVASFAAHIEVWQFSMANEFARIIQDLIEQ
                     EFTPKTGITVSFTAYPSEGFQSKVLLAMVSGDAPDIVTGPVDHLVEYGIRGSIVPLRK
                     EFREELEKIEPRLYSGAGMDHNGQGFGVVQVVGAITGFQRTDILANVGVEYPTTWAEL
                     QQIMPKLKAQGHEVAFGYGGPAASPQWGAYILMKQHGGGFVDTVNYTSLLNDPGTIAG
                     FKEYVELYTVHKMPQEINYANMFRTGDLAIFFDSITAYAAVDRSAPELAGRWSYGLMP
                     GTPRPDGTIDHQTFMSATGLAITKTSKHKKEALAFIDWLMQDDIQTKLMEELTKQMPG
                     AMWVTGNKAALSAVPLRDADRVILDKQLAVSTPYAYFPGSMVINRYLEFAVHEVLQVG
                     NTPEQALANAHNLTEQELRQKLAEYERFIKRLL"
BASE COUNT          584 a          612 c          596 g          661 t
ORIGIN      
        1 tagcggagat ggcacctggc tgagttcaga cttcatgccg tatgtagctt atcttgaaga
       61 ctttacgcct aaggactggt ttttcgatac agttctgctt cttcccggag gagagacagc
      121 ttcgagataa gctacatacg gcatgaagtc tgaactcagc caggtgccat ctccgctacc
      181 ggcgtagccg tagtaggcaa ggacaatgtc ctcagcaccg ccagtctggg cagtaccttt
      241 gggcataaag ccctttgcag gaatggcttc tggtccgaga acagcgctct tttcccagcg
      301 tttttccagg tcaagttctg gggttgactg gagctccaaa gaattggatg caatcccttc
      361 tctgccgaag acctccagct catccatgaa gacgtaaacg tctatgggga attggatttt
      421 gacgtatctt gcctgatggt tgaggccgtc taaaacaaac tcctctttct gcagtctgtt
      481 gttccaagga cctacggcag tctcaacggt ccccatgtag ctgaaattct cgttgtctgt
      541 agagaggtag tagtcgatgt agcgggggac aaagattccc gcacttctat tctgtaagaa
      601 gtgtccctta atgctgttta ccgtttgcac gtctccaaga tctacgatga tcgttcgtcc
      661 gccctgacgg tagaagcagc gccattcggc gtcctggtat ctcagggtac caaactgtcc
      721 gtctgtaagc tctctgccgt ttttgtcctc gtacttggtt tcataatctg caaaaaatgc
      781 atcgggccaa gaggctacaa cctgataggt tttccctaat gccagattga tcagtggatt
      841 ttcactggcc tttgcagccc ggggcatgaa gaaaagcacc aacagcgcaa ggagaacaaa
      901 cttatacatg tgtacacctc tgcttctcat tttttcggcg ctttaaccta attaacatta
      961 ggtgacctgc ttctggcagg tcacctatac tttttatcgg taaaactaca aaagcctctt
     1021 aataaagcgt tcgtactctg caagcttctg gcgcagctcc tgttcggtaa ggttgtgggc
     1081 attagccagt gcttgttctg gggtgttgcc tacctgcaga acttcgtgaa cggcaaattc
     1141 aaggtagcgg ttgatgacca tcgatcctgg gaagtaagca tagggagtag aaactgcaag
     1201 ctgcttgtcc agaattactc tgtctgcgtc tctcaaagga acagcagaca gggccgcttt
     1261 gttaccagta acccacatag cgcctggcat ctgctttgtc agctcttcca ttagtttagt
     1321 ctgaatatcg tcctgcatca gccaatcaat gaacgccaga gcttctttct tgtgtttgga
     1381 tgttttggtg atggcaagac ctgtagcgga cataaaagtc tggtggtcga tagtgccatc
     1441 agggcgcgga gtgccgggca taaggccgta tgaccaacgt cctgcaagct ctggggcaga
     1501 cctatctact gcagcataag cagtgatgga gtcgaagaag atcgccagat ccccagttct
     1561 aaacatgttc gcgtagttga tttcctgagg catcttgtga acggtataaa gctctacgta
     1621 ttctttaaag ccggcgattg tccctgggtc gtttaaaagg gatgtgtagt taactgtgtc
     1681 cacaaaacct ccgccgtgct gcttcatcaa gatgtaagcg ccccactgag gacttgcagc
     1741 aggtccgccg tagccaaatg ctacttcgtg tccttgagct ttgagtttag gcatgatttg
     1801 ttgaagctct gcccatgtgg tcggatactc aacacctacg ttggccagga tatcggtcct
     1861 ctggaagccg gtgatcgcgc caacaacctg gactacaccg aagccctgac cgttgtggtc
     1921 cataccggct ccggagtaga ggcgaggctc gattttctcc agctcttcac ggaactcctt
     1981 gcggagaggt acaattgaac ctctgattcc gtactccaca aggtggtcta ctggacctgt
     2041 aacgatatcg ggagcgtctc ctgaaaccat tgccaggaga actttgctct gaaagccttc
     2101 gctgggatat gcagtgaagc taacggtaat cccagttttc ggcgtgaact cctgttcaat
     2161 tagatcctga atgattctgg caaactcatt tgccatgctg aactgccaaa cctcgatgtg
     2221 cgctgcaaag gacgccactg taaacacaca aactagggca atggtaagca tgaacttgcg
     2281 cattaaaaaa ccaccttctt ttctagtatt ttccttacca cctgtatgac taataataag
     2341 tgcagagcgt ggtaaaagca actctcagga cgcaaagccc cttctaaaaa ggggctttgc
     2401 gctttttccc ttgtctacag caaattcttg ataaaccttt cgtactcttt gag
//
LOCUS       DAOSDA010000281         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_28016_length_2450_cov_3.114823, whole genome shotgun sequence.
ACCESSION   DAOSDA010000281 DAOSDA010000000
VERSION     DAOSDA010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28016_length_2450_cov_3.114823"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PLW82_06700"
     CDS             complement(<1..928)
                     /locus_tag="PLW82_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ47000.1"
                     /translation="MQDVQSDVSTQKDLRHNFKVTVAEGAAYSLMYGLTGSFIGVLAL
                     KMGASPFQLSLITALPNLMNVLLMVPVASLLERRANKVPYLTVGVFFNRIGYFFLGLA
                     AFMGLPPSVLLTVLTLMTIPAVVAGLAYTDIVANCFPPRERGRVFGAKNAVVGLLTFI
                     CTIVAGSILDKIAYPYNYFYVFGIASIFGLIDTYIMSRHRVQPATQVAKKEDQEPYLI
                     KLKNIFGHPTLGKEFQKFLITIAIVYVGLTIPNAIWTIFYVEEMQLSQFQIGNMTAIS
                     NAVMVVSSFFWGRKSQRSGDRVVFSISLLGITL"
     gene            complement(976..1269)
                     /locus_tag="PLW82_06705"
     CDS             complement(976..1269)
                     /locus_tag="PLW82_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOJ47001.1"
                     /translation="MLTSKQRAYLRGLASTEDAIVQIGKEGITDGTISSLDEALTARE
                     LVKIKVLKTVEEDPKDLAQSLVERLPGTEIIHVLGRTIVLFRMNEKKPRIILP"
     gene            complement(1378..2019)
                     /locus_tag="PLW82_06710"
     CDS             complement(1378..2019)
                     /locus_tag="PLW82_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOJ47002.1"
                     /translation="MKKHDKKQIPQRVVSRLAMYFRVLSQLDAAGVERISSRELGEMM
                     SITPSQIRQDLSYFGEFGQQGYGYRVKYLYEEICKILGLKEQHTMILIGVGNLGRAIA
                     SYTNFRNRGFYLIAAFDVREEIIGKRFNDLVIKPMHELGSFLDENKVDIAVLTVPASQ
                     AQEIANRLVEKGIKGIWNFAPIKLEVPEGVVVEHVHISDSLYSLSFRLREKTT"
BASE COUNT          654 a          575 c          534 g          687 t
ORIGIN      
        1 caagggtgat gcccagaagg gagatgctga aaactactct gtctccgctt cgctgagact
       61 tcctgcccca gaaaaacgac gaaacaacca taaccgcatt actgatagcg gtcatgtttc
      121 cgatttgaaa ctgactcagc tgcatctctt ccacgtagaa aatggtccaa attgcattgg
      181 gaatagtgag gcccacgtaa actatggcta tggtaataag aaacttctgg aattctttgc
      241 ccaaagtagg atggccaaag atgtttttca gtttgatcaa atagggctcc tggtcttctt
      301 tctttgcgac ttgagttgcc ggttgtaccc tgtgcctact catgatatag gtatcgatga
      361 gaccaaatat ggaagcaata ccaaaaacat agaagtagtt gtagggataa gcgattttgt
      421 cgaggataga gcctgcaaca atcgtgcaga taaaagtaag caggccgact acagcgtttt
      481 tcgcaccaaa gacccgtcct ctctctcggg gtggaaagca gttagccacg atgtcggtgt
      541 aagcaagacc ggcaacgaca gctggaatgg tcataagtgt caggacggtt aaaagcacac
      601 tgggaggtag tcccataaat gccgcaagtc ccaaaaaaaa gtagccgatg cggttgaaaa
      661 aaacaccaac tgtaaggtat ggcaccttgt ttgccctccg ttccaaaagg ctagcaacag
      721 gcaccattaa tagcacgttc atgagattag gaagagctgt aataaggctt aactgaaacg
      781 gtgaagctcc catcttcaac gctagcacgc cgataaaaga accggtgagc ccgtacatca
      841 aactgtaggc tgcaccttca gctactgtaa ccttgaagtt gtgacgcaag tctttttggg
      901 tacttacgtc actttgaaca tcctgcaact tatcacctct attctccatc cgaaactaac
      961 tgcttcgcta gtgttttaag gcaggattat cctcggtttt ttctcgttca ttctaaagag
     1021 aacgatagtg cggccaagaa cgtgaatgat ctcggtgccc ggcagcctct ctacaaggct
     1081 ttgggcaaga tccttaggat cctcttctac ggttttcagt actttgatct tgaccagttc
     1141 cctcgcagtg agagcttcat ccagcgagct gatcgttccg tctgtaatgc cttctttacc
     1201 gatttgcact atagcatcct cagtactggc aagccctctg aggtaagctc tctgtttgct
     1261 tgtaagcata aaagaccccc taatttgcgt ctagcgattt gaggtattgt tctcctcgat
     1321 tgtaacagag cccagaagaa cgagcaatat ggaaagcggc gctttctcag cgccgcttta
     1381 cgtggtcttt tctcgtaggc gaaagcttag tgagtataag ctgtcgctga tgtgcacgtg
     1441 ttctactaca acaccctcgg gtacttccaa cttaattgga gcaaagttcc aaataccctt
     1501 aatgcccttt tccactaaac gattggcgat ttcttgggct tgcgatgccg ggacggtcaa
     1561 aactgcaata tcgaccttgt tttcgtctag gaatgaacct agctcatgca taggcttgat
     1621 cacaagatcg ttaaacctct tacctatgat ctcttctcgg acatcaaatg cggcgattaa
     1681 gtagaaccct ctattcctga agttggtgta gcttgcgata gctctcccta ggttgccaac
     1741 accgataagg atcattgtat gctgttcttt caatcctaat attttgcaga tctcttcgta
     1801 aagatacttg actctatacc cgtatccctg ttgaccgaac tctccgaaat agctcagatc
     1861 ctgtctgatc tgcgatggtg ttatgctcat catctcgccc agttcacgag aggatatccg
     1921 ttcaacccct gcagcgtcaa gctgacttaa gaccctgaaa tacatagcaa gccgagaaac
     1981 cactctttgg gggatctgtt ttttatcgtg tttcttcaag ttgagtcccc tcccgttaaa
     2041 tatttctcaa tcctttgttt aactatttca actaaagtgg cttaattcct cgaaaaacgt
     2101 taaaaataac aattaaaaat ttttgactgg accgtaatgc ttactttaat cataagttcc
     2161 tatttcgttt aggcgcacag actgctggaa gcgtaggacc ggatgtatct tgcatttgac
     2221 ctttgacttg tttccttgca ggtcacataa ataggagcat tctcacattt cgcgggatct
     2281 gtcttgtgca tagtatgtgt tgaacatggc acgaatgccg tgtgactaca agaaaggtga
     2341 gataaaagta acatggcaga tcagtttatc cctgtatctg gatgtcctga gtatcaagag
     2401 ccaagtcgcg cttgccttat cgtcgataag gtttacgatg caagacgcac
//
LOCUS       DAOSDA010000282         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_28108_length_2445_cov_3.386611, whole genome shotgun sequence.
ACCESSION   DAOSDA010000282 DAOSDA010000000
VERSION     DAOSDA010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28108_length_2445_cov_3.386611"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(404..670)
                     /locus_tag="PLW82_06715"
     CDS             complement(404..670)
                     /locus_tag="PLW82_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOJ47003.1"
                     /translation="MAVEILKVSSKSNPNAVAGALAGVLREKGSAEIQAVGAGAVNQA
                     IKAMIICRGFVAPTGMDLVCIPAFADIAIDGEERTAMKLIVEPR"
     gene            complement(771..1571)
                     /locus_tag="PLW82_06720"
     CDS             complement(771..1571)
                     /locus_tag="PLW82_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOJ47004.1"
                     /translation="MRVLMVGDIVGRPGRKVLGYVLPKLIKDKQLDFVIVNGENSAGG
                     FGITAETFKEIKKAGADVVTSGNHIWDKRDAEDLIDSEPRLLRPANYPPGVGGRGSIV
                     VDVSGVKVGVINLAGRVYMEALDCPFRKAEEIVQEMHQAGVKLIFVDMHAEATSEKEA
                     MGFYLDGRVTAVVGTHTHVPTGDERVLENGTAYQTDLGMTGPRDGVLGVDKDIIVAKF
                     LEGTPKRFEVAKGAVWFCGLIVEADDATGKALGVERVRIEFEKDNLPW"
     gene            complement(1722..>2445)
                     /locus_tag="PLW82_06725"
     CDS             complement(1722..>2445)
                     /locus_tag="PLW82_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOJ47005.1"
                     /translation="PARIEEMVEKSRKEIESVIREQGDQATFEVGVHGLHPELVKYIG
                     RLYYRTSYGQNVLKHSIEVAHLAGLMAAELGVDVNVAKRAGLLHDIGKAADQDAEGTH
                     HQISGDLAKRYRESHEVVHAILAHHGDIEFKTVEAVLVAAADAISAARPGARRETLES
                     YIKRLEKLEEIADGFDGVDKSYAIQAGREIRIIVKPERIDDTKSVLLARDISKKIENE
                     LEYPGQIKVTVIRETRAVEYAK"
BASE COUNT          552 a          683 c          499 g          711 t
ORIGIN      
        1 ttcgtattgt tgtatcttgc cattgttaaa gggtttgaac ctctccttct tgtgggattt
       61 ttcctcctac ctacctattc tccgtgtttt ggttatttcc ttaatttatt gcggcgattt
      121 tgcataattt gtgcccgatc tgcaaaaaag ctaggggttc tccgcagtaa aaatgcgctg
      181 cttttgcgtg caacgcagct acatccgtct gctggattat cttctccagt tccggtgctt
      241 gaaacgcatc cttaacaagg gcggtcatcg gcacttgcaa gaagcggttt gcagcccctc
      301 ttttttacgg acaaaggctc aaggttcttc tttcttggct cagtctttaa aatgtcaaag
      361 ggacaggcat ggttacctgt ccctttggcg tccgttcctc aacttatctt ggctccacaa
      421 tcaatttcat tgctgtccgt tcttcgccat caattgcaat gtcggcaaat gcgggaatgc
      481 acactaaatc catcccggtt ggagctacaa agcctctgca gataatcatc gctttgatcg
      541 cctggtttac agcaccagca ccaacagctt gaatctcggc agaccctttt tctctcagta
      601 ccccagctaa ggctccggct actgcgttcg ggttagattt agaagacact ttgagtattt
      661 ctactgccac ttgtaagccc ccctatgtcc tgttattact tatattttcc gttattagga
      721 aatatccttt tagaacgttg ataaatttca atattttaat aacttctact ttaccaggga
      781 agattatcct tttcgaactc gatacgcacc cgctcaacac ccagcgcttt tccagtggca
      841 tcgtcggcct caacgatcag gccgcagaac caaacagctc ctttggcaac ctcaaaccgt
      901 ttaggggtcc cttcaaggaa cttcgcgacg ataatgtctt tgtccactcc caaaactccg
      961 tcacgtgggc cggtcatccc caagtcggtt tggtacgcgg taccattttc cagaactctc
     1021 tcgtctcccg tcggcacgtg ggtgtgggtt cccacaacgg cagtgacccg cccgtctagg
     1081 taaaaaccca tcgcttcttt ttcgcttgta gcctcggcgt gcatgtctac gaagatcagc
     1141 ttgacgcctg cttggtgcat ctcctgaaca atctcctcag ctttgcgaaa cggacagtcc
     1201 agagcttcca tataaactct gcctgcaaga ttgatcacgc ccactttgac tccgcttaca
     1261 tccacaacta tgctccctct cccacctact cccggtgggt agttggcagg acgcagaagc
     1321 ctcggctccg aatcgataag atcctctgca tcgcgtttat cccaaatgtg attgccgcta
     1381 gtgactacgt cagcgccggc ttttttaatc tctttaaaag tttccgctgt aatgccaaag
     1441 cctcctgccg agttttctcc gttgactatg acaaaatcca gctgtttatc tttaataagt
     1501 ttaggtagaa cgtaaccgag caccttgcgt ccgggacgcc ctacaatatc tccgaccatc
     1561 aaaacacgca attgacttcc tcccaacagc agataaaaaa tcttcctaaa acctgctaaa
     1621 agtataacct aaaaacacct tgatcttgta taggcacttt tccagttatg taccgctgtc
     1681 cacattgaaa attaagcgga aggatgccct tccgcttaaa cctatttcgc gtattcaaca
     1741 gcgcgtgttt ctcgaataac ggtgacttta atctgtcctg gatattcgag ctcgttctcg
     1801 attttcttag agatgtctcg agcaagcagc acgcttttgg tatcgtcgat ccgctcgggt
     1861 ttgacgatga ttctaatctc gcgtccagct tgaatcgcat aggatttgtc tacaccatcg
     1921 aaaccatcgg ctatctcttc aagcttctct agacgcttaa tatatgactc aagtgtctct
     1981 cttctggcgc ccggtcttgc ggcgctgata gcatctgctg ctgcaaccaa aactgcctca
     2041 acagtcttga actcgatatc accgtggtgg gccaaaatcg cgtgaacaac ttcatgcgat
     2101 tctctgtagc gtttggccaa atcgccactg atctggtggt gggtgccttc agcatcctga
     2161 tcggcagcct taccaatatc gtgcaggagt ccggctctct tcgctacatt gacatctacg
     2221 cccagttcgg cggccattag gcctgctaaa tgagcaacct ctatagaatg cttcaggaca
     2281 ttctgtccat agctggttct atagtagagc cttccaatat acttcaccag ctcgggatgc
     2341 aagccgtgaa cgccaacttc aaaggttgcc tgatcaccct gctcgcggat gacgctttcg
     2401 atttccttcc gcgacttctc taccatttcc tcgatgcggg cagga
//
LOCUS       DAOSDA010000283         2440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_28202_length_2440_cov_4.541300, whole genome shotgun sequence.
ACCESSION   DAOSDA010000283 DAOSDA010000000
VERSION     DAOSDA010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28202_length_2440_cov_4.541300"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLW82_06730"
     CDS             <1..391
                     /locus_tag="PLW82_06730"
                     /inference="COORDINATES: protein motif:HMM:NF036850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47006.1"
                     /translation="GVEVIVASRRKPEESFWNRFPWEELRRDLVVDADLVVNCTPCGM
                     YPNVDDELIVNPASFPKKTCFCDIVYNPLKTRFIQVAQETGHKTVTGEGMLLFQGAIS
                     FTKWYGQEAPIGVMKQVLLSYLQGGEN"
     gene            392..1147
                     /locus_tag="PLW82_06735"
     CDS             392..1147
                     /locus_tag="PLW82_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ47007.1"
                     /translation="MIESRELTKKFGNVTAVNSVSFDVKEGELFAFLGPNGAGKTTTI
                     KMLSGLLIPTSGAAFIDGVSVIDNPVEAKKRIGFVPDQPNVYDKLTLREFLDFVISIY
                     RVDRKKAQETAEEYLEIFDLADRQDELLEGYSHGMRQKANIIAALIHEPKVMFLDEPT
                     VGLDPRSARLIKDILRKLTAEGRTIFMSTHILEIAEEMADRVGIINKGSLKFIGTVEE
                     LRKAQGKDGSLEDLFLELTASEEDKELISQLRG"
     gene            1153..>2440
                     /locus_tag="PLW82_06740"
     CDS             1153..>2440
                     /locus_tag="PLW82_06740"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47008.1"
                     /translation="MQEYLLLLRNSVRVWFTSSFKGKSKKKAFGKVFGYLAIIGVAVM
                     WTVFSARAFGFLRDLILPENAAMEGAMLEAGGILLQTMLVTVAQLLSLATLMFLFLTG
                     MRVIFEYLYDSPDVHFLLATPLKVENIFAAKFTECLALIFISISFMTLAPMIGLGITF
                     KAGFAYYLVMVISFLAGFVLFGSLSALFLLLIMRYVPGQRLKKVLMSLTLVMALVIVF
                     ITQVFSSGMMNITEEELVNVIYGLSDLGLNKASFLPHVWMAKSSIAALPSSTINLWAN
                     LLPLLLVSSLVFWLTVKVSARLYLAGFAAGTEVDVQKKKKAVKAEPKKEVKTASPFGA
                     MFRKEYVYLKREPMMWYQLAVGLIVMGFYAFNMTRNAPPPGEVGMTMDTGMMSLSLFM
                     IIFFAGITGPNTAGLSLSREGQNWRFLQSLPIEPKTI"
BASE COUNT          622 a          563 c          626 g          629 t
ORIGIN      
        1 gggggttgag gtgatcgttg ccagcagaag aaagcccgag gagagtttct ggaacagatt
       61 cccttgggaa gaactccgca gggatctggt cgtggatgcg gatttggtgg taaactgcac
      121 accgtgtgga atgtacccta atgttgacga cgaacttatc gtcaaccccg caagctttcc
      181 taagaagact tgcttttgcg atatcgttta taacccactg aaaaccaggt ttatccaggt
      241 tgcccaggaa acaggacaca aaacggtaac gggagaaggt atgctgcttt tccagggagc
      301 gatctcattt acaaaatggt atggtcaaga agcgccaatc ggcgtaatga aacaggtgct
      361 actcagctac ttgcaaggag gagagaactg aatgattgaa tccagagaac tgacgaaaaa
      421 gttcggcaat gttaccgcag tgaacagcgt ttcctttgac gtaaaagaag gagaactttt
      481 tgctttccta gggcctaacg gcgctggaaa aacaaccacc atcaagatgc tttcaggcct
      541 gcttatcccc acaagcggag ctgcatttat tgatggcgtc tcagttatcg acaatcctgt
      601 agaagctaaa aagcggatcg gctttgtgcc cgatcagccc aacgtgtacg acaagcttac
      661 actaagagaa ttcctggatt ttgtgatcag catctaccgc gttgaccgca aaaaggcaca
      721 agagacggct gaggagtacc tggagatctt tgacctagcc gatcgccaag acgaacttct
      781 ggaaggctac tcccacggta tgcggcagaa agctaacatc atagccgcac tcatccacga
      841 accgaaagta atgttcctgg acgaacccac tgtaggactg gatccccgtt ctgccagatt
      901 gatcaaggat atcctgcgga agcttactgc tgaaggcaga accattttca tgtccacgca
      961 catcctggag attgccgagg agatggcaga tcgagtcggg attattaata aaggttctct
     1021 gaagtttatc ggtactgtcg aagagctgag gaaagcccaa ggtaaagatg gctccttgga
     1081 agacctgttc ttggagctta ctgcaagcga agaagacaaa gaactcatca gccagcttag
     1141 ggggtgagag ggttgcaaga atacctactg cttttaagaa acagtgttcg cgtctggttt
     1201 acatcatcat ttaaaggcaa atcaaaaaag aaagcctttg gaaaggtatt tgggtatctt
     1261 gccatcatcg gcgttgcggt catgtggacc gttttctcag cccgcgcttt tgggttcctc
     1321 agggatctga ttctaccgga aaacgcggca atggagggag cgatgctaga agccggcggc
     1381 atcctgttgc agacgatgct ggttacagta gcgcagctct tgagccttgc aactcttatg
     1441 tttctcttcc ttacgggaat gcgggtcatt ttcgaatacc tgtatgactc tcccgatgtc
     1501 catttccttc tggccacacc tcttaaggtg gaaaacatct ttgcagcaaa attcaccgag
     1561 tgtttggcct tgatctttat atccatcagc tttatgaccc ttgctcccat gatcgggctg
     1621 ggcattacct tcaaagctgg gtttgcctac tatctggtga tggtcatcag cttccttgct
     1681 ggctttgtgc tctttggctc gctgtcggca ctgtttctgc tgctcatcat gaggtatgtc
     1741 cccggacagc ggttaaagaa ggtgcttatg tccctcacat tggtgatggc cttggtgatt
     1801 gtctttatta cccaggtgtt ctcttctggt atgatgaaca tcacagaaga agagctcgtc
     1861 aatgttatct acggtttgag tgacctgggc ttgaacaaag caagctttct acctcacgta
     1921 tggatggcaa aatccagtat cgccgcttta cccagctcta ccatcaacct gtgggctaac
     1981 ctcttaccgc tgcttttggt gtccagctta gttttctggt tgacggtgaa ggtctcggcc
     2041 agactctatc ttgcagggtt tgccgcaggc accgaagttg acgtccagaa gaagaagaaa
     2101 gctgtgaaag ctgagcccaa aaaggaagtt aaaacagcca gtcctttcgg ggctatgttc
     2161 aggaaggaat acgtgtatct caagcgcgaa ccgatgatgt ggtaccagct ggctgtggga
     2221 ttgatcgtta tgggttttta tgccttcaat atgacgagaa atgccccacc tcctggagaa
     2281 gtcggaatga cgatggatac gggaatgatg tctctcagcc tgtttatgat catcttcttt
     2341 gcaggcatta cgggaccaaa cacagcggga ctgtctcttt ccagggaagg tcagaactgg
     2401 aggttcctgc agtctctacc tatagagcct aaaacgattt
//
LOCUS       DAOSDA010000284         2431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_28350_length_2431_cov_3.608165, whole genome shotgun sequence.
ACCESSION   DAOSDA010000284 DAOSDA010000000
VERSION     DAOSDA010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28350_length_2431_cov_3.608165"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /gene="pstC"
                     /locus_tag="PLW82_06745"
     CDS             complement(<1..826)
                     /gene="pstC"
                     /locus_tag="PLW82_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOJ47009.1"
                     /translation="MKNCERFAKTTSLSKATSLEDISPKVKLERIMHIIFLCSGFICV
                     VAVVLISLYMVISGLPVIREVGLKNFLFGTVWDPLNSTYGILPFVLSSLLATGCAVLV
                     GGLFGLAVSVYLVFLAGQRVASMLRSLVQLLAGIPSVVYGLLGAMLIVPLVFRIQTFF
                     GLPTSGSLLAAIFVLVIMILPTIISVSENALRAVPKQYMDGSYALGASKIQTILKVMI
                     PSAKSGIASGLVLGVGRAIGETMAVMMVAGNASLLPELLKPARLLTVGISIEWAYSS"
     gene            complement(1009..1854)
                     /locus_tag="PLW82_06750"
     CDS             complement(1009..1854)
                     /locus_tag="PLW82_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ47010.1"
                     /translation="MKKGILIVLGLVLLVLLAFTFASKNKPTALTGVVKTGGSTSVEK
                     VMNSLIYQFQLYNRDVRIDYEMNGSGDGIKSTLSGLYEIGHSSRDLKTDGSESGLKAI
                     PYAIDGIAIVVNKDNPVENLTKKELFGIYTGKITNWSDVGGKDALITVVSREQGSGTR
                     SAFSETVGLDLKERPETAQVTSAIITDSTGAVQTTVMQNPNAIGYMSFSDVDRDKVKT
                     VSYNGVEISEETLRSDTYKLKRTFLMVVKEGAELSGSAQAFLDFVLSDEGQKIVAANN
                     LIPVK"
     gene            2100..>2431
                     /locus_tag="PLW82_06755"
     CDS             2100..>2431
                     /locus_tag="PLW82_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47011.1"
                     /translation="MYKKLFALICALLMFSAFALAEDILIYEANFTKDDDPSKVNWVS
                     YDGDWSIESGLMVNYDTSSQNTAITQELDQSKEGIYIYEYKMSYFETSVTWGPMSGLH
                     FMASQPDGA"
BASE COUNT          630 a          612 c          570 g          619 t
ORIGIN      
        1 ccgatgagta agcccattcg atggatattc ctacagtaag gagccgggca ggtttcaata
       61 gctcgggcaa tagtgaagcg tttcccgcaa ccatcatcac cgccatggtc tcgccgatgg
      121 ccctccccac tcccaggacc aggccgctgg cgatcccgct tttggcgctg ggaatcataa
      181 ccttcaagat ggtttgaatc tttgatgcac ccagggcata cgacccgtcc atgtactgtt
      241 taggcaccgc tcgtagagcg ttttccgaaa cgctgatgat ggtaggaaga atcatgatta
      301 ccagcacgaa aattgccgca agaagcgacc cgcttgtggg aagcccgaaa aaggtttgga
      361 tcctgaagac cagaggtacg atgagcatgg ctcccagaag tccatagacc accgaaggaa
      421 tcccagcaag cagctggact aaagagcgca gcattgacgc gactctttgg cctgccaaaa
      481 acaccaagta cactgacacc gcaagtccga aaaggccgcc cacaagcacg gcgcatccag
      541 tagccaaaag cgaagacaga acaaacggta aaatgccgta ggtggagttc agtggatccc
      601 agacggtgcc aaagaggaag ttcttcaacc ccacttctct gatcacaggc agtccggaaa
      661 tcaccatgta cagggagatt aagaccactg ctacaacgca gataaagcca gaacagagaa
      721 agatgatgtg catgatgcgt tctagcttta ccttagggct tatgtcttct aggcttgtgg
      781 cttttgaaag agatgttgtt ttcgcaaaac gttcgcagtt tttcatcttc tactcctttc
      841 catttttggc taatgcttcc aaatggaact ttgctttatc tacagatagt ctattaaccg
      901 gtttttgtcc taaatgccaa tggcgcaaaa gcctacagcc tctaggtaat atgttcttgg
      961 tccgagccga aaggccgcgc tatagcgggc tgtgcccgca gcaccagatc acttcactgg
     1021 aatcaggttg tttgctgcaa cgattttttg cccctcatcg ctcagcacaa agtccaaaaa
     1081 tgcctgagcc gatcctgaaa gctccgcgcc ttcttttaca accatcagaa acgtccgttt
     1141 tagcttgtag gtgtcggatc tcagggtttc ttcggagatc tctacgccgt tgtagcttac
     1201 ggtcttgact ttatccctgt ccacgtcgga aaaagacatg tagccgatgg cgtttggatt
     1261 ctgcataact gtagtctgaa ctgctcccgt tgagtcggta attattgccg atgtaacctg
     1321 cgcggtttcc ggtctctctt tcagatccaa gcccacagtc tccgaaaacg cgcttctggt
     1381 gccggagccc tgttctctgg agacaacggt aatcagcgca tccttcccac cgacatcgct
     1441 ccagttggtg atctttccgg tgtagatgcc gaaaagctct ttcttggtaa ggttttctac
     1501 tgggttgtcc ttgtttacta caattgcaat gccgtcgata gcgtagggaa ttgccttaag
     1561 tccggactca gaaccgtctg tcttcaggtc cctgctggag tggccaattt catagaggcc
     1621 gctgagggtg ctcttaattc cgtcgcctga accgttcatt tcgtagtcga tacgcacgtc
     1681 tctgttgtag agctggaact ggtagatcag tgagttcatc actttctcga cggaagtgga
     1741 accgccggtc ttaacaactc ctgtaagtgc tgtaggtttg ttcttcgaag cgaaggtgaa
     1801 cgctaaaagc acaagtagta ccaaacctaa aacgatcaga ataccttttt tcatgtttac
     1861 ttcctccttg tccaatgtca tcttacaaga gcttgcctgg cgaaaacagc aaatccacct
     1921 attggttatc tatattttgt gtaaagttgc agacgcaaaa ctctacgatg cacaaaaaaa
     1981 ccgcctcata aggcggatcc cggaaaaaat tgtctgcaag aaaggtagat ctagcaggat
     2041 aaatcagttg tttaatgaat tagaaaaatg aatgaaaaac taggtggggg ggtatttaca
     2101 tgtacaaaaa gctttttgcg ctcatctgcg cactcctaat gttttccgct tttgcattag
     2161 ctgaggacat tctcatctat gaagctaact tcacaaaaga tgatgatccc agcaaagtaa
     2221 actgggtttc ctacgacggc gactggagta tcgagagcgg tcttatggtc aactacgaca
     2281 cctcgtcgca aaacaccgcc attactcagg agcttgatca atcgaaggaa ggcatctaca
     2341 tctacgagta caagatgagt tacttcgaga cctctgtaac ctggggtcct atgagcggtc
     2401 tccactttat ggccagccaa cctgacggcg c
//
LOCUS       DAOSDA010000285         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_28512_length_2421_cov_3.026205, whole genome shotgun sequence.
ACCESSION   DAOSDA010000285 DAOSDA010000000
VERSION     DAOSDA010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28512_length_2421_cov_3.026205"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLW82_06760"
     CDS             complement(<1..216)
                     /locus_tag="PLW82_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47012.1"
                     /translation="MNIPILTVEEKSEYLQRIARPSLLDLAESFPPSRATEPIFGKAM
                     KPEEAVRSILRKVRTEGDKGVMEVARVL"
     gene            complement(213..851)
                     /gene="hisG"
                     /locus_tag="PLW82_06765"
     CDS             complement(213..851)
                     /gene="hisG"
                     /locus_tag="PLW82_06765"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017814860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOJ47013.1"
                     /translation="MLTIALPKGRLMDKVLELFAEAQIIDSKELCEQSRKLIIEDPKA
                     NFRYILAKPIDVPTYVEHGVADLGVVGKDILIESERLVYELMDLGIGKCRMIVAVSDQ
                     SNLNEVKELGFSAKVATKYPNITTSFFRSHGIQSEIIELNGSIELAPLVGLADAIVDI
                     TETGTTIRENGLRIIGEVFPISARLIANHVSYKLHYKKLQDIQEKLEGVVQC"
     gene            complement(975..2090)
                     /gene="hisZ"
                     /locus_tag="PLW82_06770"
     CDS             complement(975..2090)
                     /gene="hisZ"
                     /locus_tag="PLW82_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOJ47014.1"
                     /translation="MLFEIPKGTKDFLPLEAKWKKDIEDNLYNILSSWGYRQVIPPTY
                     EYLNTLMQGEQHENYFSFFSEDGHTFALRNDFTTPIARLATIALSDEPRPLRLSYMGN
                     LFRLRRKERRREFWQAGAELIGDGSKAADAEILALAMDALDKLGIRGWNLDISHRGVV
                     EGILEDSNFGSAAKKQIEALLVKKDYADLKGLVEKEDTGHDLLLLPKLRGGPEILTVA
                     KEKLTSTKAKGALDEIAGILDALKPYSLGAVNIDLANAKDFSYYTGMFFDAYAPGVAS
                     VICTGGRYDKLLGNFGTPEPAVGLAIGIDEVMAVLSEQNHRDTANSIPDLGILYSSES
                     YEKAVSLAVEKRREGLSVFIAPCGEEAAPPAKKYLKV"
     gene            complement(2090..2395)
                     /locus_tag="PLW82_06775"
     CDS             complement(2090..2395)
                     /locus_tag="PLW82_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HOJ47015.1"
                     /translation="MAALEKIRDEQVELLVSAILALKDRDECYRFFEDICTIAEIKAM
                     AQRIAVAKMIQDGETYTEISEKTGASTATISRVKRSLHYGADGYNTILARLRDEGVI"
BASE COUNT          548 a          633 c          540 g          700 t
ORIGIN      
        1 caggactctg gctacttcca ttacaccttt atcgccctcg gttctgacct tgcggagaat
       61 gctccttact gcttcttcgg gcttcatagc ctttccgaag atcggctctg tcgcccgaga
      121 aggagggaat gattctgcca agtccaagag gctcggtctg gcgatcctct gtaggtattc
      181 gctcttttct tctacggtca agatgggaat gttcaacact gcactacccc ctcaagtttc
      241 tcttggatat cttgaagctt tttgtaatgg agcttgtagc tgacgtggtt ggcaatcagc
      301 cgtgcggaga tcgggaaaac ttctccgata attctaaggc cgttttccct aatggtggtt
      361 cctgtttcgg taatgtcaac gatggcatct gcaagcccca caagaggtgc aagttcgatg
      421 gagccgttga gttctatgat ctccgattgg attccgtggc ttctgaaaaa gctggtggtt
      481 atgttaggat acttggttgc caccttagca ctgaatccga gttcttttac ttcatttaga
      541 ttggactggt cgctcacagc aacaatcata cggcatttgc cgatacctaa atccatgagt
      601 tcgtacacca agcgttcgct ctctatgaga atgtccttgc caaccacacc gagatctgcc
      661 actccatgct caacatacgt aggtacgtca atgggcttgg ccaggatgta gcggaaatta
      721 gcttttgggt cttcaatgat cagctttctg gactgctcgc aaagttcttt cgagtctatt
      781 atttgggctt ctgcaaaaag ctccagcacc ttgtccatca gccgaccttt tggaagtgca
      841 attgtcagca tgaaaagttc tccttttgtc ctttatcttg gtaaagtgtt aacttgtaaa
      901 aatactacca tagccatttg ccagtgtcaa gaaaaaaaga aaggtggtag cacctttctt
      961 aagaacagtc gtcttcaaac tttcagatac ttcttcgctg gaggcgcggc ttcttctccg
     1021 catggagcaa tgaagacact caacccttct ctgcgctttt ccactgccag agagaccgct
     1081 ttttcgtagc tctcagagct gtacaggatg cctaggtccg gtatggagtt tgctgtgtct
     1141 ctatggttct gctcggatag tactgccatg acttcgtcaa tccctatcgc caagccaaca
     1201 gcaggttctg gggtgccaaa attgccgaga agtttatcat accttccccc agtgcagatg
     1261 acgctggcaa ctccaggagc gtaagcgtca aaaaacatac cggtgtagta gctgaagtcc
     1321 ttagcgtttg caagatcgat gttgacggca cctaagctgt acggctttaa ggcatcgaga
     1381 atccctgcaa tttcatccaa agccccttta gctttggtcg atgtcaactt ctctttagcc
     1441 accgtcagaa tctccggtcc accgcgaagc ttaggaagaa gcaagaggtc gtgccctgtg
     1501 tcttccttct ccactagccc tttcagatcg gcatagtcct ttttcaccag caacgcctca
     1561 atctgctttt tcgccgcgct gccgaagttg gaatcctcta agatcccttc cactactccc
     1621 ctatgactga tatccaagtt ccaacctctt atccccagtt tgtccaaggc atccatcgca
     1681 agagccagga tctctgcatc agcagctttc gagccatcgc cgattagttc tgctcctgcc
     1741 tgccagaact cccgtctacg ctctttgcgc cgaaggcgaa agaggttgcc catataggac
     1801 agcctaagag gacgaggctc gtctgacaga gcaatggtcg ccagcctcgc gatcggtgtg
     1861 gtgaagtcgt tccgaagagc aaaagtatgg ccgtcttcac taaagaagct gaagtagttt
     1921 tcgtgttgct ccccctgcat taacgtgttc aagtactcgt acgttggcgg aataacctgg
     1981 cggtatcccc aagacgacaa gatattgtaa agattgtctt cgatatcttt tttccacttt
     2041 gcttccagag gcaggaagtc tttagttccc tttggtattt cgaaaagcat tagatcacac
     2101 cttcatctcg tagtcttgcc aggatggtat tgtaaccgtc tgcaccgtag tgcagtgacc
     2161 gctttaccct gctaatggtt gcagtggatg cgccggtttt ttcgctgatt tccgtataag
     2221 tttctccatc ctggatcatt ttcgctaccg caattctttg agccatggct ttgatctctg
     2281 caatggtgca gatatcctca aagaacctgt agcactcatc tctatctttg agggccaaaa
     2341 tcgccgaaac caagagttcc acttgttcat ctctgatttt ttctaaagca gccattggat
     2401 acccccccgt tctattttaa c
//
LOCUS       DAOSDA010000286         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_28572_length_2417_cov_3.249365, whole genome shotgun sequence.
ACCESSION   DAOSDA010000286 DAOSDA010000000
VERSION     DAOSDA010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28572_length_2417_cov_3.249365"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLW82_06780"
     CDS             <1..412
                     /locus_tag="PLW82_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ47016.1"
                     /translation="PPQVKAIIEDCGYTSVDAQLSYQLSRMYHLPSFPLLNTTSLVTK
                     LKAGYSFSEASALEQIKKNKLPILFIHGAEDWFVPVSMVYELYEACPSEKELYIVEGA
                     GHGAAYGQNPEEYERRVADFLKKYLEETPKEGTA"
     gene            complement(430..1818)
                     /locus_tag="PLW82_06785"
     CDS             complement(430..1818)
                     /locus_tag="PLW82_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47017.1"
                     /translation="MSVELSLLHWVYAFFVAAIIAFMVMRKDTSFICILGIAVLGLMA
                     TGTVSGAISGIFNGFAYAIVELLGTILIISIIVAMSKVLSETGINGVMIAPFAKLIRT
                     PALAYWVIGIVMMVISWFFWPSPAVPLIGAILLPVAIKVGLPALGVAVAMNLFGHGIA
                     LSGDFIIQGAPKLTSSAAGLPVGEVISASIPLVIVMGLVTTTVAFYLLMRDIKAKKLV
                     AKEEVLETIKENAPSLISRRAQVILAVVVPILFLLDLVVMLTLKLQGGDATALVGGTA
                     VFILVLISLLAHKKKGLEKITSYLIEGFQFGFRIFGPVIPIAAFFYLGDAGFLSIFGD
                     LLPAGSQGLVNDLGFALAQTVPLNSIVGAITLTLVGAITGLDGSGFSGISLAGSVGKV
                     FGIALNASPATLTALGQIAAIWVGGGTLIPWAVIPAAAICGVDPFELARKNLVPVVIG
                     LAVTTVFAIFLI"
     gene            complement(1990..>2417)
                     /locus_tag="PLW82_06790"
     CDS             complement(1990..>2417)
                     /locus_tag="PLW82_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ47018.1"
                     /translation="IVAFPCALLFGRLSKKFKTEDLIKVCIVGYFLITAFALQLDKAW
                     EFWTLAVFVAVFQGAIQALSRSYYAKIVPKEKSSEYFGIYDIFGKGASFAGTTMMGVV
                     TQLTGSSKYAVGAISIMFVIGYIVFRLNTKIERAAAVES"
BASE COUNT          683 a          637 c          606 g          491 t
ORIGIN      
        1 ccctcctcag gtcaaagcca tcatcgagga ctgcggttac acctcggtag atgcccagct
       61 gtcctaccag ctgagcagaa tgtatcacct tcctagcttc ccactcctca acactactag
      121 cctcgtaaca aaactgaaag cgggctacag cttctccgaa gcttctgcgt tggagcagat
      181 caagaagaac aagcttccca tcctctttat tcacggagct gaagactggt ttgtgccagt
      241 tagcatggtc tacgaactct atgaagcttg tcccagcgaa aaagagctgt acatcgtaga
      301 gggagcaggt cacggagcgg cctacggcca gaaccctgaa gagtacgaga gaagagttgc
      361 cgacttcttg aaaaaatacc tagaagagac acctaaagag ggaactgcct gaggcagttc
      421 cctcctgcgt tatatgagaa atatggcaaa gactgtagtc accgcaagac cgataactac
      481 cggcaccagg ttttttcgcg ctagttcaaa gggatccact ccgcagatgg cagctgcagg
      541 aatcacagcc cagggaatca atgtcccacc accaacccaa atggccgcta tctgaccgag
      601 agcggtgagg gttgctggac tggcgttcag tgcaatgccg aaaaccttgc ccaccgagcc
      661 ggcaagagag atgccggaaa agcccgagcc gtcaagaccg gtgatcgcgc cgaccaaagt
      721 cagagtgatg gctccaacaa tggagtttag cggcaccgtc tgggccaaag caaaacccaa
      781 gtcgttcacg agcccctgag atcctgcagg aagcaaatcg ccgaagatag agagaaatcc
      841 cgcatccccc aggtagaaga aagctgcaat ggggattacc ggtccgaaga tcctaaagcc
      901 aaactgaaag ccttcgatca ggtagctagt gatcttctcc aagccctttt tcttatgggc
      961 aaggagggaa ataaggacca gaatgaagac tgccgtacct cccacaaggg ccgttgcgtc
     1021 tcccccctgg agctttaaag tgagcatgac tacaagatcc agtaaaaaga ggatcggcac
     1081 caccactgcc aaaatcacct gagcacgtct ggagatgagg ctaggtgcgt tttctttgat
     1141 ggtttccaac acttcttctt tggcaacaag cttctttgct ttgatgtccc tcattaaaag
     1201 atagaaggcc accgtggttg ttacaaggcc catcactatc accaatggga tgctggcgga
     1261 gatgacctcc ccgactggca gtcctgcagc agacgaagtg agtttcggtg cgccctggat
     1321 gataaagtca ccagagaggg caataccgtg gccgaagagg ttcatagcga cagcaacgcc
     1381 tagggcggga agtcccactt tgattgcaac aggaaggaga atggctccga tgaggggaac
     1441 agcaggcgac ggccagaaga accacgaaat caccatcatc acgatgccta tgacccagta
     1501 ggccagagcc ggcgtgcgga tcagtttggc aaacggcgca atcattacac cgttgatgcc
     1561 cgtttcagag agcaccttac tcatggcaac gatgatggag ataatcaaga tcgtgccgag
     1621 aagctctaca atggcgtaag caaagccgtt aaatattcca gagatagccc ctgacacggt
     1681 tcctgtagcc attagaccca gtaccgcaat gcctaggata cagatgaagc tggtatcctt
     1741 gcgcatgacc ataaaggcga taatggctgc aacaaaaaac gcataaaccc agtgcaggag
     1801 acttagttcc accgacatag ctaacctcca ctttcctgga agcggcacag aaaagccaca
     1861 ggcaccaggc cgcttcccaa aacttgtgcg tgacattata cactatgtcg ggaccagaag
     1921 tatggttaaa gaatatcaga ttcctaatgt gaaaaagtcc cgccttttgg cggaactcta
     1981 ctatgcagtt cagctttcta cagctgcagc tctttctatt ttggtgttga ggcggaagac
     2041 tatatagccg ataacaaaca ttatggaaat cgcccctact gcgtacttgg aacttcccgt
     2101 aagctgggtg acgaccccca tcatggtagt gccggcaaaa gacgctccct tgccgaaaat
     2161 atcgtagatg ccaaagtact cgctggactt ttccttagga acgattttag catagtagct
     2221 cctggaaagg gcttggatag caccttggaa caccgctaca aacaccgcaa gggtccagaa
     2281 ctcccaggct ttatccagct gcagggcaaa ggcagtgatg aggaagtagc ccacaataca
     2341 gactttgatc aggtcttcgg tcttgaactt cttggagagc ctgccgaaaa gcagtgcaca
     2401 cggaaaggca actatct
//
LOCUS       DAOSDA010000287         2415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_28603_length_2415_cov_3.145339, whole genome shotgun sequence.
ACCESSION   DAOSDA010000287 DAOSDA010000000
VERSION     DAOSDA010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28603_length_2415_cov_3.145339"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PLW82_06795"
     CDS             complement(<1..886)
                     /locus_tag="PLW82_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ47019.1"
                     /translation="MNFTEAKTLAEKYLMHAYNPYPLVLAEGKGAYLYDTEGKAYLDF
                     LSGIAVNSLGYGHEGVLNAIFTQAAKLMHTSNFFYTEPTLRLAEKLSQLSGLEEGKVF
                     FCNSGAEANETAFKLARLHGKGKGKNKVVACRGSFHGRTMGSLTLTGQSKYHDGFEPL
                     VPNVVHTEFNNVEALAKDLTSDTCALFVECIQGEGGVVPATKEYLKKARELCDQLDIL
                     LVIDEVQTGVGRTGTFFAYEGFGVKPDIVTMAKALGGGLPIGAVIASGKAAVFTPGKH
                     GTTFGGNAVAASAALAVLD"
     gene            complement(887..1753)
                     /gene="argB"
                     /locus_tag="PLW82_06800"
     CDS             complement(887..1753)
                     /gene="argB"
                     /locus_tag="PLW82_06800"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOJ47020.1"
                     /translation="MTNEHDFNKVNLLVEALPYIKALTGKTVVVKYGGHSMETPEVKK
                     AVILDLILLKYVGMNPVVVHGGGPEINSYMEKLGIEPQYHKGLRITSKEVMEIVQMVL
                     IGKVSQELTSMINHYGGKAISISGKDGNTIIARKKLVEGVDLGYVGEVDKVNPELIES
                     VIAAGYIPVISPVGVGLDGESYNINADSVAGEVAAALKAEKLIFLTDVEGIYEDPQNP
                     SSIFTALQAQRARRLIESGAISKGMIPKVEACIKALEFGVNRTHIIDGRQPHTMLLEI
                     FTDAGIGTMVVN"
     gene            complement(1765..>2415)
                     /locus_tag="PLW82_06805"
     CDS             complement(1765..>2415)
                     /locus_tag="PLW82_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOJ47021.1"
                     /translation="IHPNMATLLAFITTDWPVESFTLQSMLKEIADETFNAISVDGDT
                     STNDMCLVLASKELPKPDPEKLEIFRQGLKQVMAELAKAIVMDGEGATKLLKICVKGA
                     KSVCDAKLAAKAIGNSPLVKTAFFGEDANWGRILCAVGYSGCTVDPDKTDLYFEDIQI
                     VKSGQGLGISDAALLPIMKQREIEITVDLGLGNGEYVFQTTDFSYDYVKINASYRS"
BASE COUNT          525 a          641 c          596 g          653 t
ORIGIN      
        1 gatccaaaac ggcaagggca gcgcttgcag caacggcatt gccgccaaaa gttgtgccgt
       61 gctttccggg ggtaaagacc gccgctttac ctgaagcaat cactgctcct ataggaagtc
      121 cccctcctaa ggctttagcc atggtcacga tatcgggctt cacgccaaaa ccttcataag
      181 caaaaaacgt accggttctg cctactccgg tctgcacttc atcaataaca aggaggatat
      241 ccagctggtc gcagagctcc ctagcttttt ttaggtactc cttggttgct ggcaccactc
      301 caccttcacc ttgaatgcac tctacaaaca gagcacaggt gtcggaggtg agatcttttg
      361 caagagcctc cacgttgttg aactcggtgt ggaccacatt tggcacgaga ggctcgaaac
      421 catcgtggta cttgctctga ccggtgagag ttagggaacc catagtgcgg ccgtggaagg
      481 aacctctgca tgctaccact ttgttcttgc ccttcccctt tccgtgcaag cgggcaagct
      541 tgaacgctgt ttcgttggct tcggctccgc tgttgcagaa aaagaccttc ccttcctcaa
      601 ggcccgaaag ctgagagagc ttctcagcca agcggagcgt gggctctgta taaaagaagt
      661 ttgatgtgtg catgagtttt gccgcttggg tgaaaatggc attaaggacg ccttcatgtc
      721 cgtagccgag actgtttacg gcaataccgc tcaaaaaatc caggtaggct ttgccttcag
      781 tgtcgtagag gtatgcccct tttccctctg caagaaccag gggatagggg ttataagcat
      841 gcattaaata cttttctgca agtgtcttgg cttcggtaaa gttcatttag ttcaccacca
      901 ttgttccgat tccagcatct gtgaagatct ccagaagcat agtgtggggc tgtctgccgt
      961 cgataatgtg ggtccggttc acgccgaact ccagagcttt gatacatgct tccactttgg
     1021 gaatcatgcc tttggagatg gcgccggact cgataagacg tcttgcccgt tgagcctgga
     1081 gtgctgtaaa gatcgaagat ggattttgag ggtcttcgta gatgccctca acatctgtga
     1141 ggaagattag cttttcggct ttaagagctg ctgccacctc tcctgccacg ctgtctgcgt
     1201 tgatgttgta ggactcgccg tcaagtccca cgccgacagg actgatcacg ggaatgtagc
     1261 ctgcagcgat cacgctctcg atgagttcgg gattgacctt atccacctca ccgacataac
     1321 ccagatccac cccttcgacc agtttcttcc tggcgataat ggtgttgcca tctttcccgc
     1381 tgatgctgat ggccttgccg ccgtagtggt tgatcatgga tgtaagttcc tgactgacct
     1441 tacctatgag caccatctgc acgatttcca tgacttcctt gctggttatt ctaagtccct
     1501 tgtgatactg gggctcgatt cctagcttct ccatgtagct gttgatctcc ggaccaccgc
     1561 cgtgcactac caccgggttc atgccaacgt atttcagaag aatgagatcg agaataaccg
     1621 cttttttcac ctcaggggtt tccatagagt ggccgccata tttaacaacc accgtcttgc
     1681 ctgtgagggc tttaatatag ggcagagcct caacaagcag attcactttg ttgaaatcat
     1741 gttcatttgt catttggttt cctcctagct tctgtagctg gcgttgatct tcacgtagtc
     1801 atagctgaaa tccgtcgttt ggaagacgta ctctccgttt cccaagccga gatcgacggt
     1861 gatctcaatc tcccgctgtt tcatgatagg tagtagcgca gcatcgctga tgccaagacc
     1921 ctgtccgctc tttacaatct gaatgtcttc gaagtaaagg tcggtcttat ccggatcgac
     1981 ggtgcatcca gaataaccta cggcgcagag gattctgccc cagttggcat cctccccgaa
     2041 gaaagcagtc tttaccagtg gtgagttgcc gatggccttt gcggcaagtt tggcatcgca
     2101 gacgcttttt gcgcctttca cgcagatctt tagtaacttg gtcgcgcctt ctccgtccat
     2161 gacaatggcc tttgccagct cggccatgac ctgcttcaaa ccctgtctga agatctccag
     2221 cttctccgga tcgggcttag gcagttcctt tgacgcaagc accaagcaca tatcgtttgt
     2281 ggatgtatct ccgtccaccg agatagcgtt gaaggtctcg tcagcgatct cttttagcat
     2341 gctttgcaaa gtgaagcttt caacaggcca gtcggtggta ataaaagcca gcaaggttgc
     2401 catgttggga tggat
//
LOCUS       DAOSDA010000288         2402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_28833_length_2402_cov_4.086493, whole genome shotgun sequence.
ACCESSION   DAOSDA010000288 DAOSDA010000000
VERSION     DAOSDA010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28833_length_2402_cov_4.086493"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..333)
                     /locus_tag="PLW82_06810"
     CDS             complement(118..333)
                     /locus_tag="PLW82_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47022.1"
                     /translation="MKKDLVAIIGSIDDLTKRAEELEERLDKLESPEHRQELEVIKNE
                     LSKLKADVEAQLLLLQEELDRIEKSLI"
     gene            complement(466..1521)
                     /locus_tag="PLW82_06815"
     CDS             complement(466..1521)
                     /locus_tag="PLW82_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ47023.1"
                     /translation="MVVRIGVVGAGYWSEECHVPGLKLADEAEVVALSGRRVENTKSQ
                     AARLSIPKVYTDYRTMLEEEELDAITITTPNSTHREIALAALEKGLSVFCEKPLALNA
                     EEAAEMAAAAGKLGLVNHVGFTFRFLQSSIKAKELLAEGFLGHLFHARLWAENDSGLI
                     VRPLAWRDDKTLAGTGQLGDMVSHIFDLFRYVTGDEFTAVYAHSRAVQPKREAGRTEA
                     LATVDDFNSLMFETERGVSGIAVASRVTRGLGGWSVEFFGTKGAMRIVYSRGWKDELW
                     YAPHGEEYRLMATESIMGDFAMLHMMEAFVAEIAGRKPLTNGIPATFNDGLRAQEVID
                     AVLLSAVKDAKVAVRTV"
     gene            complement(1538..>2402)
                     /locus_tag="PLW82_06820"
     CDS             complement(1538..>2402)
                     /locus_tag="PLW82_06820"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HOJ47024.1"
                     /translation="SEAVTGTIASAVTKSIAREDRSLKEVLPGEVLEKVPSWTDSISQ
                     SILKSIEDYLGKPSAEELFKKHIDGFLAKGPLRNLLGMLLDTSLISRQLVGTVLQLVK
                     DPETKLQVFSAVESGINELLEKKPQEVLAAFGEGEVDKAVSSGLKLLFSERTVDAFCR
                     REYLEKLITSPEIGEKIAVALKRAATKAKGMRIQSIYRLYGDGNWQELKKKVLSVYKK
                     KAPDLLVRVFSEFNLTKIIEDKINTFDLLELENLIFGISKRELGAITWLGGLLGFLMG
                     LLTPLINALLL"
BASE COUNT          556 a          650 c          518 g          678 t
ORIGIN      
        1 ttactatgtg gttgtcaaag tgcacaataa ctattgacta ttcatagcac gtctcgtttc
       61 tttcctcttg ttgttctcct aaaaaaagag caccagcttg cgctggtgtt cttcttttta
      121 gatcagactt ttttcgattc tgtccagttc ttcctggagc agaagcagct gggcttcaac
      181 atcagctttc agcttggaaa gctcgttttt gatcacttca agttcctgcc tatgctcagg
      241 gctttccagc ttgtcgagac gctcttccag ttcctctgct cttttggtaa gatcatcgat
      301 cgagccgatg atcgcaacga gatctttttt caaaacagtt cctcctacat cttggtcaac
      361 tctacatata tttacttaag taatgctcaa tcctgcaaag acaccaaaaa tccatcaaaa
      421 aattcttgtt ttcttctttc ttgaaacgcc tccgccagaa cgaagctaca ctgtccttac
      481 tgcaaccttg gcatctttta ccgcagagag caaaaccgca tcgatcacct cttgggctct
      541 aagcccgtcg ttaaaggtgg cgggaatacc attagttaga ggctttcttc ctgcgatctc
      601 cgcaacaaac gcttccatca tatgcagcat ggcaaaatct cccatgatgc tttctgtggc
      661 cataaggcgg tattcttctc cgtgaggagc gtaccataac tcgtccttcc accctctaga
      721 gtagacgatt ctcatggctc ctttagtgcc aaaaaactct acagaccaac ctccaagccc
      781 tcgggtcacc ctgcttgcga cagcaatgcc ggagactcct ctttcggttt caaacatgag
      841 cgagttgaag tcatctactg tggccaaagc ctcagtgcgg ccggcttctc tcttgggttg
      901 aactgccctg ctgtgggcgt aaactgcagt aaactcgtca cctgttacat accgaaagag
      961 atcgaagatg tgagacacca tatcccccag ctgtcccgtt cctgcaaggg tcttgtcgtc
     1021 acgccaggca agcggcctta cgataaggcc tgaatcattt tcagcccaga gccgtgcgtg
     1081 aaacaggtga ccgaggaagc cttctgcaag cagttcctta gctttgatgc tgctctggag
     1141 aaacctgaag gtaaagccca catgattaac cagtcctagc tttccagctg cagctgccat
     1201 ttccgctgct tcctctgcgt ttaacgccag aggtttttcg caaaacacgg aaaggccttt
     1261 ctctaaagca gccagagcga tctccctgtg ggtactgttg ggggtagtga tggtgatcgc
     1321 atcaagttcc tcttcttcca gcatcgttct gtagtcggtg tagactttgg gaatggagag
     1381 cctggctgcc tgagacttgg tattctccac tctccttcct gaaagggcaa ctacttctgc
     1441 ttcatcggca agcttcagac ccggaacatg gcattcctcg ctccagtagc ctgctccgac
     1501 aactccgatt cttactacca tgcttgtcca ccatcctcta gagtaatagt gcgttgatta
     1561 aaggcgttag aagtcccatc aaaaatccca aaaggcctcc cagccaggtt atagcgccca
     1621 gctctctctt ggatatgcca aagatcaagt tctccagctc caagagatcg aaggtattga
     1681 tcttgtcttc gatgattttt gtcagattga actcgctgaa aaccctaacg agaagatcag
     1741 gtgctttttt cttgtaaaca gagaggactt ttttcttcag ctcctgccaa ttcccatccc
     1801 cgtacagtct gtagatgctc tgaatcctca tccccttagc tttggtagca gcgcgtttga
     1861 gagctacagc tatcttttcc ccgatttctg gagaagtgat gagtttttct agatactctc
     1921 tccggcagaa agcatcaact gtcctttcgg aaaagagaag tttaaggcct gagctgacgg
     1981 ctttgtcgac ttctccttca ccgaaggcag cgagcacttc ctgcggcttt ttctccagca
     2041 actcatttat cccgctttca acggcactga agacctggag ctttgtctcc ggatctttta
     2101 ccaactgcaa aacggtgccc acaagctgcc ttgatatcag agaagtgtcc agaagcatac
     2161 ccaagaggtt ccgaagaggt ccttttgcca ggaagccgtc gatgtgcttt ttgaaaagtt
     2221 cttcggcaga aggttttccc aggtagtcct cgatgctttt gagaatactt tgcgaaatgg
     2281 agtctgtcca agagggcacc ttttctagca cctcacctgg cagaacttcc ttgagactgc
     2341 ggtcttcacg ggcaatgctt ttagtcacag cactggcgat ggtaccggtt acagcctcgc
     2401 tt
//
LOCUS       DAOSDA010000289         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_28889_length_2399_cov_3.766638, whole genome shotgun sequence.
ACCESSION   DAOSDA010000289 DAOSDA010000000
VERSION     DAOSDA010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28889_length_2399_cov_3.766638"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1263)
                     /locus_tag="PLW82_06825"
     CDS             complement(271..1263)
                     /locus_tag="PLW82_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HOJ47025.1"
                     /translation="MVLGFGRRPAVKNFQEEYEKEKVVARRNLGVLEVEVDKIVGSVG
                     RWRDFDRKFQLQTTHSPMRLRSIEAAMITGKELPPVDLYKIKDEYYIVDGNHRVAAAR
                     RLGWKRIKARVTEYLPSADTPEGILTRERSYFCFKTGLADINLTEIGHYQTLLKEIED
                     YKAYLQERYKEEYTIKEAAKKWHEDVYMPLADIIEKDKLIEEFPGRTTSDLFLYIVEH
                     KKRLTASEGREIPLIEALREFCAPSPKSLTQTIMDLFTRLTVSKQQCISCDRCIKICP
                     VDAIVKDGGVYHILDHCIKCNRCVPVCPTDAIKPSSGSEVPSIIQEVKDKEEKK"
     gene            complement(1328..1915)
                     /locus_tag="PLW82_06830"
     CDS             complement(1328..1915)
                     /locus_tag="PLW82_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ47026.1"
                     /translation="MKILIVSDKEVPNLQEGSGGEILQGVDMIISCGDLRSGYLNSIS
                     NRANAPLFYVAGNHDFHYYYERPKGTNLDSRIVTFRDLRLAGFEGSIRYNHKTYQYTE
                     YEMKKRVKRVARKLMFNPKIDIVVTHAPPFGIYENVDECHTGFQVYRWFIEKYQPAYF
                     LHGHYHLDYGMNIEREHIIGNTRVINCVGYYVLEI"
     gene            complement(2055..>2399)
                     /locus_tag="PLW82_06835"
     CDS             complement(2055..>2399)
                     /locus_tag="PLW82_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47027.1"
                     /translation="LQWFLSAEVQSKLASKVAQEMPGAVFLPANINAIARLAIPEDHI
                     RAFSTQAQEARSPAYALLPEAKAQKLINLAVYETVIGGISPEEALRKAADKLTLELDQ
                     KRKEYARFIEKL"
BASE COUNT          552 a          574 c          559 g          714 t
ORIGIN      
        1 tcttgctaaa gtttccataa gtatcacctc ggtcttgtct tcgatgctgc agttgcgtgg
       61 caaacacctg cagacaagaa actccttgag gtaggtgcac gaaagcctct ttgcacccac
      121 ttcgtttctt ttattatagc agaatgcttc ctgcaaggta ggtagcaggc atttacttga
      181 aaaagaaggg cagctgtttc agctgccgcg gtaaatagac caaaaaggag caccgcttaa
      241 taggcagtgc tccttttgcg tgcaaacgag tcacttcttt tcttccttgt cctttacctc
      301 ctggatgatg gaggggacct cgcttccaga tgaaggcttg atcgcatctg taggacacac
      361 aggcacgcag cggttgcact tgatgcagtg atctaggatg tgatagacgc caccgtcttt
      421 gacaattgca tctaccggac agatcttgat gcagcgatca caactgatgc actgctgctt
      481 ggaaacggtc agcctggtga aaagatccat gatagtctgg gttagcgatt tcggtgacgg
      541 tgcgcagaac tctctgagag cctctatcag cgggatctct cgaccttcgc ttgcagtgag
      601 cctctttttg tgttctacga tgtagagaaa caggtccgat gtggttcggc cagggaactc
      661 ttcgatcagc ttatctttct cgatgatgtc cgcaaggggc atgtagacgt cttcgtgcca
      721 cttctttgcc gcttctttga tggtgtactc ttccttgtac ctttcctgca gataagcttt
      781 gtaatcttcg atttctttca gaagcgtttg gtagtgaccg atctcagtga ggttgatgtc
      841 tgcaagtcca gttttaaagc aaaaataaga ccgttctcta gttagaatac cttcaggagt
      901 atctgcagaa ggcaggtact cagtaaccct agctttgatc ctcttccagc ctaagcgtct
      961 tgctgcagcg acacggtgat taccgtctac gatgtagtat tcatctttga tcttgtacag
     1021 atccacaggc ggcagttctt ttccggtaat catcgccgct tcaatggagc gaagccgcat
     1081 aggactgtga gtagtctgca gttgaaactt gcggtcaaaa tctcgccagc gtccaacact
     1141 acccacaatc ttatctactt caacttccaa gacgccaaga tttctccgag ctacgacttt
     1201 ttctttctca tattcctctt ggaaattctt tactgcaggt cttctgccaa agcctaatac
     1261 cataatttaa gacgagctct tgggttgagc tggacttgtt caccaccttt ttagcgagat
     1321 agcgggttta gatctctagt acatagtagc caacgcagtt tatcaccctc gtgttgccga
     1381 tgatgtgctc tctttcaatg ttcataccgt agtcaagatg gtaatgcccg tggaggaaat
     1441 acgcaggttg gtacttctca atgaaccagc ggtacacttg gaacccagtg tggcactcgt
     1501 caacgttttc gtaaatgcca aagggcgggg cgtgagtaac gacaatatca atcttagggt
     1561 tgaacatcag ctttctggca acccgcttca cccgtttttt catctcgtac tcggtgtact
     1621 ggtaggtttt gtggttgtag cggatgctgc cttcaaagcc cgccaagcgg agatccctga
     1681 aagtcacaat tctcgaatct agattggtgc ccttagggcg ctcgtagtaa taatgaaaat
     1741 catgattgcc agccacgtag aaaagcggtg cgttggctct atttgatatg gagttgaggt
     1801 acccggatct gagatcgccg caggagatga tcatgtcaac tccctgcagg atctcaccac
     1861 ctgaaccttc ctgcagattt ggtacctctt tgtcactgac aattagaatt ttcatgcttt
     1921 tcgctccgct ccaattcgat aattttataa cgtgttttta cttaattcac ttttcgcagg
     1981 tgttaaacct ctctaatgcg aaaaaaagcc ggccaccttg ttgattgaac tcgaatgtgg
     2041 ccggtttatt tgctttaaag cttttcgata aatcgggcat attctttgcg tttttggtcc
     2101 agttcaagtg taagcttgtc tgctgctttt cgcagcgctt cttctggact tatgccacca
     2161 ataaccgttt cgtaaactgc taggttgatg agcttctgcg ctttagcttc tggaagcaac
     2221 gcataagcag gagatctggc ttcttgtgcc tgagtggaga atgctcggat gtggtcttct
     2281 ggaatagcca gtcttgctat ggcatttata ttggccggga gaaacaccgc acctggcatt
     2341 tcctgagcca ctttgcttgc cagcttgctc tggacttctg cagataaaaa ccactgcag
//
LOCUS       DAOSDA010000290         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_29018_length_2391_cov_3.012414, whole genome shotgun sequence.
ACCESSION   DAOSDA010000290 DAOSDA010000000
VERSION     DAOSDA010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29018_length_2391_cov_3.012414"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..587
                     /gene="rplK"
                     /locus_tag="PLW82_06840"
     CDS             162..587
                     /gene="rplK"
                     /locus_tag="PLW82_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOJ47028.1"
                     /translation="MAKKVLAKVKLQIPAGKANPAPPVGPALGQHGVAIMEFCKQFNA
                     RTQNQAGMIIPVEITIFADRSFTFITKTPPAAVLLKKAAGLEKASGSPKAVKVGKVTK
                     EQLREIAEMKMPDLNAADVEAAMKVIAGTARSMGITIEE"
     gene            651..1358
                     /gene="rplA"
                     /locus_tag="PLW82_06845"
     CDS             651..1358
                     /gene="rplA"
                     /locus_tag="PLW82_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOJ47029.1"
                     /translation="MAKKLGKRYSASLQTFDRHEVYDVKEAMELVIKTATAKFDETIE
                     AHIRLGVDPRHADQNVRGTVVLPHGTGRSVRVAVFAKGDKAKEAEESGADIVGAEDLA
                     EKIQGGWLDFDIAVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDIAKAIKDIKAG
                     KVEFRVDKQAIVHVPIGKASFGPEKLEENFLALMSAIIKAKPAAAKGNYLRKIALAST
                     MGPGVRVSTADVLNTAR"
     misc_feature    1380..1536
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1558..2079
                     /gene="rplJ"
                     /locus_tag="PLW82_06850"
     CDS             1558..2079
                     /gene="rplJ"
                     /locus_tag="PLW82_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOJ47030.1"
                     /translation="MSKQTNEAIVQEITQKFAEAKGAVLIDYRGLTVNQDTELRKKLR
                     AAGVDYRVYKNTLLSIAAKNQGIEGLDQYLAGPTAIAFGMVDAVAAPKILRDAIKDFK
                     KMEIKGGVLDNKVIDAKQVEALADLPSKEQLLAMLVRGMQAPITGLVNCLQGNIRNLV
                     YVLEAIRKQKEGA"
     gene            2131..>2391
                     /gene="rplL"
                     /locus_tag="PLW82_06855"
     CDS             2131..>2391
                     /gene="rplL"
                     /locus_tag="PLW82_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOJ47031.1"
                     /translation="MAFDKDAFIKQIEEMTVLELAEVVKALEDRFGVTAAAPVAMVAG
                     AAPAAAPEAAEEKTEFDAILTDTGAEKIKVIKVVRELTGLGLK"
BASE COUNT          687 a          524 c          616 g          564 t
ORIGIN      
        1 agtatcttgt gaagtaatct caagctcagg caggaaaaca aaaaggctga agccggtctt
       61 actctagtct ggacaaaatc tacatcgtag aaactactta accataggtt gaaactatag
      121 gttggagttt tagttagcgc tcttgtctgg gaggtgaaac aatggccaaa aaagtactcg
      181 ctaaagtaaa gctgcagatt cctgctggta aagccaatcc agcacctcca gtaggtcctg
      241 ctcttggtca acatggtgtg gcaattatgg agttctgcaa gcagttcaat gctcgcacgc
      301 agaatcaggc aggtatgatc attcctgttg aaatcaccat ttttgccgac agatcattta
      361 cgtttatcac aaagacccct cctgctgcgg tactgctgaa gaaagctgct ggtttggaga
      421 aagcatccgg aagtcctaag gctgttaagg ttggtaaagt caccaaagaa cagctcagag
      481 agatcgccga aatgaagatg ccagacctca acgcagccga cgttgaagcc gctatgaagg
      541 ttatcgctgg caccgctcgc agtatgggca ttacaattga agagtaagag ctcttcggaa
      601 gaccagtggg agaagttaaa cttcgttaac accacaagga ggaaagttta atggctaaaa
      661 aacttggtaa aagatactcc gcttccctcc agacgttcga tcgccatgag gtgtacgatg
      721 tcaaggaagc tatggagctt gtgatcaaaa ctgcaacagc caaattcgac gaaaccattg
      781 aagctcacat ccgcttgggc gtagaccctc gccacgcaga tcaaaacgtt cgtggtactg
      841 tagttctgcc tcacggcacc ggccgctctg tcagagttgc tgttttcgct aaaggcgaca
      901 aagccaagga agcagaagaa agcggagcag acatcgttgg tgctgaggat cttgcagaaa
      961 agattcaggg tggttggctt gactttgata tcgccgttgc cactccagac atgatgggcg
     1021 ttgtaggccg tctcggtaga gttctcggtc ctaaaggctt gatgccaaac cccaaatccg
     1081 gcaccgttac attcgacatt gccaaagcaa ttaaggatat caaagccggt aaggtcgagt
     1141 tcagagttga caagcaggcg attgttcacg tgccaatcgg caaagcttca ttcggtccgg
     1201 agaaactcga agagaacttc ctggcactga tgagcgctat tattaaggcc aaacctgcag
     1261 cagcaaaggg taactatctg agaaagattg cacttgcctc aacgatgggc ccaggcgtca
     1321 gggtaagcac agccgatgtt ttgaacactg caagataact tactcgatac taaaccaata
     1381 gaatagaaac ctctctgcca gagacagcag gtccgatgca gctcatctgc tagggtaagt
     1441 atcatacgcc tgccgaggta gtttaggata gatcgtttta gacgtgtgat ctgtcgcgaa
     1501 ctctcatctg gtgggttcgc gttttttgtt aggcaggagg tctaaggagg tgaaatcgtg
     1561 tcgaaacaaa cgaacgaagc aatagttcaa gaaataactc aaaagttcgc agaagctaaa
     1621 ggtgcagttc tgatcgatta tcgcggcttg acggttaatc aggatacgga actgagaaag
     1681 aaactccgcg cagctggagt tgactatcgc gtttacaaga acacactcct tagtattgcc
     1741 gcgaaaaacc agggtatcga aggtcttgat cagtatcttg ccggcccaac agccatcgct
     1801 tttggtatgg tagatgccgt tgcagcccct aagattctcc gcgatgccat caaagacttt
     1861 aagaagatgg agattaaagg cggtgtcttg gacaacaagg tcatcgatgc caagcaggtt
     1921 gaggctcttg ccgatcttcc atccaaagag cagctcctgg ccatgctggt tcgtggtatg
     1981 caagccccga ttacaggtct ggtcaattgc ctgcagggca atatcagaaa cctggtgtac
     2041 gttttggagg ctattcgcaa gcaaaaagaa ggcgcctgat ttggtgctgg taatctaaaa
     2101 acttaacaaa tctaggaggt aatatttaag atggcttttg ataaagatgc tttcattaag
     2161 caaatcgaag agatgacagt tttggagttg gctgaagttg taaaggcatt ggaagacaga
     2221 tttggcgtaa ccgctgcagc tcctgtagca atggtagccg gcgctgctcc tgccgcagct
     2281 cctgaagcag cagaagagaa gaccgagttt gatgcaatcc ttacagatac cggtgcagag
     2341 aagatcaagg ttattaaggt cgtcagagag ttaaccggtc ttggacttaa a
//
LOCUS       DAOSDA010000291         2386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_29114_length_2386_cov_3.509224, whole genome shotgun sequence.
ACCESSION   DAOSDA010000291 DAOSDA010000000
VERSION     DAOSDA010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29114_length_2386_cov_3.509224"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLW82_06860"
     CDS             <1..262
                     /locus_tag="PLW82_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOJ47032.1"
                     /translation="DDLFRFVREKGAFLWAATLKDAKPYYGLTPKTGIGIVIGNEGAG
                     ISREIAQKCDEKVYIPIDDRAESLNAGIAAGVLLFHLRKELV"
     gene            397..1383
                     /locus_tag="PLW82_06865"
     CDS             397..1383
                     /locus_tag="PLW82_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47033.1"
                     /translation="MEEPWEALQRKELIKHLPAAAFSFFVALFVLILQHLPPLLFSLV
                     LNDFLKTGKLRFLAGAIIVTCLIPVALFKLEKRYKDWILAECSVRAKALHELVLISIM
                     EKPLEFFADGKPVAVYKNHGASCSCFVDMLLVVLPRLVAKGVAAFLLLFKVGSVLPTV
                     AWLQLLFVVLMVVVELVKKGAVESQSSTDDIALRQMFQNAKVFTSSLKTLGLGSTKIE
                     RFADDLEDTDPLFKVQETDRQEWAGSVQVLRGIFTAISFGIGVKALAAQAILGGDLLL
                     IMVYLPALHALLLDLTTVRLPAFSFYQPLFQFLTVSTEKTGDLEGNKPESIR"
     gene            1590..1691
                     /locus_tag="PLW82_06870"
     CDS             1590..1691
                     /locus_tag="PLW82_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HOJ47034.1"
                     /translation="MLNADFFWRVFELTGSLVAYLMYRECKAKARME"
     misc_binding    1671..1902
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1944..>2386
                     /gene="pheS"
                     /locus_tag="PLW82_06875"
     CDS             1944..>2386
                     /gene="pheS"
                     /locus_tag="PLW82_06875"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014909.2"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOJ47035.1"
                     /translation="MNENINKLREEVWLELEKIADLEALEKFRVKYLGKKGAITALLR
                     SMGSLPKEERPLFGKAVNELSTEVEEKLEEKEAQLMEAKRARQLAEEALDVTLPGRAP
                     RLGHRHPLKQVMEEIEEIFLNMGYTIADGREVEDDFHNFEALNIP"
BASE COUNT          636 a          500 c          621 g          629 t
ORIGIN      
        1 tgacgacctg ttcaggtttg ttagagagaa aggcgctttc ctttgggctg caacccttaa
       61 ggacgctaaa ccctactatg gcctgacacc taaaacgggg atcggcatcg tcattggcaa
      121 tgaaggtgcg ggaatcagtc gcgaaattgc acaaaaatgc gatgagaaag tctatatccc
      181 aatcgacgac agggcagaat cgctgaatgc cggaatagct gctggtgtcc tattgtttca
      241 cctgagaaaa gaactagttt gacctcaacg ctgcaggttc gtcctgcagc tttttttgag
      301 gacaacaaac cgctcgtttc ctggtcatgt tagacaagct cttccaatat gaatcagtat
      361 aggttttcta tgatttaacc gcaggaggct atgaccatgg aagagccttg ggaagcgctg
      421 cagaggaagg aacttataaa gcatcttcca gctgccgctt tttcgttttt tgtggctttg
      481 tttgtcctga tcctgcaaca ccttccgccg ctcctattca gtctggtact gaacgatttt
      541 ctgaaaaccg gcaagctgcg ttttttggcc ggagctataa ttgttacatg cttgataccg
      601 gttgccctgt tcaagctgga gaaaaggtac aaagactgga tacttgccga gtgcagcgtg
      661 agggcaaaag ctctacacga acttgttcta ataagcatca tggaaaagcc tttagagttt
      721 tttgccgatg gaaagcctgt tgcagtctac aaaaaccatg gagcatcgtg cagctgcttt
      781 gtcgacatgc tgcttgtggt gctacctaga cttgtagcga agggggttgc tgcttttctg
      841 ttgcttttca aggtaggttc ggtgcttcct actgttgcct ggttgcagct actctttgtt
      901 gttcttatgg tcgtcgtgga actggtgaaa aaaggtgctg tggaaagcca aagcagtaca
      961 gacgatattg cgttgaggca gatgtttcag aatgccaagg ttttcaccag tagtctgaaa
     1021 acccttggtt tgggaagtac taagatcgag cgttttgctg atgacctgga agatacagac
     1081 cctctcttta aggtacaaga aaccgatagg caggaatggg ccggttcagt ccaagtgctt
     1141 cgaggtattt ttaccgcgat ttcattcggg atcggcgtga aagcattggc agcacaagct
     1201 attttaggag gagatctgct cctaataatg gtttaccttc ccgctttgca cgctctgctg
     1261 ctggatctta ccacagtcag actgcctgcc ttcagctttt atcagccgct cttccagttt
     1321 ttaaccgtgt ctacagagaa gactggagac ttggaaggaa ataagccgga aagtattagg
     1381 taagaagtcg gtaggcacag cactaaactt tacaaagcgc ttgcataggg ctttcttggc
     1441 tttaaactgc agaagtatgt catcaagttt tacaagaaga gacaaaatgt ggacacgctc
     1501 cagaaaagcc ttttgcttga gtagggtttt aaactgtgtt ataataaacc atgaaaacat
     1561 agcggctttg tgaaagggtg gggctcctca tgttgaatgc tgatttcttt tggcgagtgt
     1621 ttgagctcac cgggtcgtta gtagcatatc tcatgtatag agaatgtaaa gcaaaagcta
     1681 ggatggagta aagtactata tgcagcgcct tcagggacag aggatcggag actgaaagtc
     1741 ctctgggtca gctgcattta ggaagttcgc tccggagctg cagcctgaag ccgcgtttcg
     1801 tggtgcgtag gagctgccgg tacactccgt tatcgtgcaa tgagagaaga actgattcag
     1861 ttcttaatca gggtggtacc acgggtctaa ctcgtccctt gcgagttaga ccatttgttt
     1921 tttaaagagg aggtagaggt ctgatgaatg aaaacatcaa caagttaaga gaagaagtct
     1981 ggctagagct ggaaaagatt gccgatctgg aagctctaga aaagtttcgc gtaaaatacc
     2041 ttggcaaaaa aggtgccatc acagccctgc tccgttccat ggggtctctc ccaaaagaag
     2101 aaagaccgtt gtttggcaag gctgtaaacg agctgtcgac tgaagtggaa gagaagcttg
     2161 aggaaaaaga agcacagctg atggaagcaa aaagagcaag acagcttgct gaagaggctc
     2221 tcgatgtaac tcttccggga agagctccga gactcggcca cagacaccct cttaagcagg
     2281 ttatggaaga gatcgaggaa attttcctta atatgggtta caccatcgcc gatggccgcg
     2341 aagttgagga cgatttccac aacttcgaag ccctcaacat tccgaa
//
LOCUS       DAOSDA010000292         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_29447_length_2365_cov_3.749784, whole genome shotgun sequence.
ACCESSION   DAOSDA010000292 DAOSDA010000000
VERSION     DAOSDA010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29447_length_2365_cov_3.749784"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PLW82_06880"
     CDS             complement(<1..551)
                     /locus_tag="PLW82_06880"
                     /inference="COORDINATES: protein motif:HMM:NF037218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOJ47036.1"
                     /translation="MGFFNKLVQGLTKTRNNLVNQIKSVLRGRKISEELFEELEEVLI
                     TADIGVKSTLEIVENLRERCREEKIQDGEEVKNLLKEELKKLLGDGSGLKAPNAEPWV
                     IMIVGVNGVGKTTTIGKLAAKFTSQGKKVVLAAGDTFRAAAAEQLGIWAERSGAQLIR
                     HAEGSDPAAVAFDALQAARNRKA"
     gene            complement(567..>2365)
                     /locus_tag="PLW82_06885"
     CDS             complement(567..>2365)
                     /locus_tag="PLW82_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ47037.1"
                     /translation="LKGVLGLLADFIDFAPPYDKDRSFLQILAEFLGGRTFVADNLEN
                     ARALAKACRTRYRIVTLAGEVIWTGGTMTGGEDKQERIGLLQRQAVTRNLEAEIKAET
                     EALEKDRKNLENLKAEVEKKSQELTELHDLIRSKTTGLEALRANSTALDEKIAAADLR
                     LQSSLQEKKRLEEQALEVKAQLKQVEESIAKLEEEQGVLKIRLSELGQMEWTEEAGIR
                     EQYQNLKEQETIAKERVLASRRSRDELVLAINQTEDLIAAQHRELEQIMLAEVRERLN
                     ALNKSKLRLVLLFQSEMVRKVIKGLKDEKASVLEKLNAFEVEIAKLRPEISKAETYLR
                     DVTVAYAKLESQIENCQQKAEELGIALEKASDEHAIPIQERNKLNQVVRKLDREIASL
                     GVVNVGAIEEYKEVSERYQFLSTQINDLTCAKDDLMEVLAQLDRQSREKFLEAFNEIK
                     VEFEKLFKHLFGGGQATLLLEEGDVLEAGIEIKAQPPGKKLQSIALLSGGEKTLTAIA
                     LVFAAVSVRNVPFYLFDEVDASLDEANLVRFINLVKEKAEKAQIILITHRRRSMEEAN
                     LLYGVTMEEKGVSKVLALHLEDQAAAAMYESI"
BASE COUNT          468 a          664 c          533 g          700 t
ORIGIN      
        1 tcggctttgc ggtttcttgc cgcctgcaac gcatcaaaag ccacagcagc aggatcggag
       61 ccttcggcat gtcggatcag ctgggcgcca gatctttctg cccatatgcc gagctgctca
      121 gcagcggcag ccctgaaagt atctcctgca gccagcacga ctttcttgcc ctggctggtg
      181 aacttggctg caagcttgcc gatggttgtg gtctttccaa caccgttgac gccaacgatc
      241 ataattaccc agggctcggc gttgggcgcc ttcaacccag agccgtctcc aaggagcttc
      301 ttcagctctt cttttaggag gttttttacc tcctcaccat cttggatctt ttcttccctg
      361 cacctttctc tgaggttctc tacaatctcc agggtgctct taactccgat atctgctgtg
      421 atcagcactt cttctagttc ctcaaagagt tcctccgaga tctttctccc tctgaggacg
      481 ctcttgatct gattcactaa gttgtttctg gtttttgtca gtccttgaac aagtttatta
      541 aaaaaaccca cggtatcact ccttggttag atagattcgt acatagctgc cgcagcttga
      601 tcttcaaggt gtagtgccag aaccttactt acacctttct cttccatggt aaccccatac
      661 aggaggttgg cttcttccat ggaacgacgg cgatgggtga tgagaataat ctgcgctttc
      721 tcggcttttt ccttcaccag gttgatgaag cgaacgagat tggcttcgtc aaggctggca
      781 tctacctcgt cgaacaggta gaatggcacg tttctcaccg aaactgcagc aaacaccaac
      841 gcgatggcgg taagagtctt ttcgccaccg ctgagaaggg ctatactctg caactttttg
      901 ccgggaggtt gtgccttgat ctcgattccc gcttccagaa catcgccttc ttcaagaaga
      961 agagttgcct ggccaccgcc gaaaaggtgc ttgaagagtt tttcgaactc gaccttgatc
     1021 tcattaaaag cttctaagaa cttctccctg ctctggcggt caagctgcgc caaaacttcc
     1081 ataagatcgt cttttgcaca ggtaagatcg ttgatctgag tgcttaaaaa ctggtagcgc
     1141 tcggagacct ctttgtattc ttcaattgcg cctacattga caactcccag agaagcgatc
     1201 tctctgtcga gttttcgaac cacctggttc agtttatttc tttcctggat aggtatagca
     1261 tgctcatcgg aagctttttc aagcgcaatg cccagctctt cagccttttg ctggcagttc
     1321 tcgatttggc tctccagctt ggcataagct acggtcacat cgcgcaagta ggtctcggcc
     1381 ttggatatct caggcctgag ctttgcgatc tcgacttcga aagcattcag cttttccagc
     1441 acagaagcct tttcatcttt caggcctttg atgacctttc tgaccatctc gctttgaaac
     1501 agcagtacaa gcctgagctt actcttgtta agtgcattca gcctttctct tacctctgcc
     1561 aacatgatct gctccagctc gcggtgctgc gcagcaatga gatcctcagt ctggttgatg
     1621 gcaagaacga gctcatcccg gctccttcta gaggcaagta ccctttcctt cgctatggtt
     1681 tcctgttcct taagattttg atactgctcc cggataccgg cctcttcggt ccattccatc
     1741 tgtcctagct cgctcagccg tattttgaga accccctgct cttcttcgag ctttgcgatg
     1801 ctctcttcca cctgcttcag ctgagctttg acctccaagg cctgctcttc caagcgtttt
     1861 ttctcctgca gtgagctttg gagcctaagg tctgctgcag cgatcttttc gtcgagagca
     1921 gtggagtttg ctcttaaagc ctccagcccg gttgtcttgc tccggataag gtcgtggagc
     1981 tcggttagct cctggctttt cttttctacc tctgctttaa gattttccag gttcttcctg
     2041 tctttttcca gcgcttcagt ctcggctttg atctctgcct ccagatttct ggtgacagct
     2101 tgccgttgta aaagccctat gcgctcctgt ttgtcctcgc cgccggtcat ggtgccgcca
     2161 gtccagatca cttctcctgc caatgtgacg atgcggtatc tggtccggca cgctttggca
     2221 agggctctag cattttccag gttatcggca acaaacgttc tgccgccgag aaactcagct
     2281 aagatctgca gaaacgaccg gtctttatcg tatggcggag caaaatcgat gaaatctgca
     2341 aggagtccca agacaccttt aaggg
//
LOCUS       DAOSDA010000293         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_29606_length_2356_cov_3.247718, whole genome shotgun sequence.
ACCESSION   DAOSDA010000293 DAOSDA010000000
VERSION     DAOSDA010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29606_length_2356_cov_3.247718"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLW82_06890"
     CDS             complement(<1..575)
                     /locus_tag="PLW82_06890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ47038.1"
                     /translation="MNRHLKKGKVGLNTWLLATSCFLAYTSCYAGRSILSAIMPQMIA
                     ETHFTRGDLGTMGSAFFLTYGAGQLVNGVLGDRIGAKHMVPFGLFFTGILLALFSPLE
                     STSLGVLLWAFCGLLLSMLWGPLSKVIAENTSTEVGTAILTSLNVASILGTSSAYMTA
                     AYGASRGSFRSAFLASAVVLTATALIWYVILT"
     gene            complement(572..1528)
                     /locus_tag="PLW82_06895"
     CDS             complement(572..1528)
                     /locus_tag="PLW82_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47039.1"
                     /translation="MKKYSLFREGSFYKGNLHTHTTVSDGKLSPLETIDRYKKNGYSF
                     LGISDHNVFSYYPEAEEENFLSIPAVEAHTSKGGSGIHHVVAFADPKTMKFANGHKLD
                     QHFCNGLTTQELVDYLREYNNMVIYCHPHWSRDEIGDIVDLKNIVGMEIYNHGCQVEW
                     RCGFAEVFYDHVLWHGNYIWNFATDDAHGATASSDYCGGYITVKTCDFSHKGILDAIS
                     KGSFYSSAAREGEAAPEILDFLVEDGLVKLWCTPTRSVCFFAGIKSHPIIFGGYGTKS
                     FQGTKDSPITYAEYQLPEGTKFVKVSAEDFSGSVSWSQPILL"
     gene            complement(1914..>2356)
                     /locus_tag="PLW82_06900"
     CDS             complement(1914..>2356)
                     /locus_tag="PLW82_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ47040.1"
                     /translation="QKPLVLAYVVDEGKDKMVSGEAALAVVWSGDAAYAIEENPDLAY
                     TFPRTGTNLWFDVVAIPKGAKNIDAAHKFIDFLNRPDIAFRNVDHIGYATPHLAAKDQ
                     LPKEITDDPDFYPTDEDLVNAEVFVNLGATLKEYDRIWTEVKAH"
BASE COUNT          573 a          546 c          652 g          585 t
ORIGIN      
        1 gtgaggatta cataccaaat cagtgctgtt gcggtaagaa ccactgcaga ggccagaaag
       61 gcgctgcgaa acgaccctct agatgctccg taggctgcgg tcatgtaggc agaagaggtg
      121 ccgaggatcg aagctacgtt caaagaggtg agtatagcag tacccacttc tgtgctggtg
      181 ttttctgcga ttaccttgga aagaggtccc cacagcatgg aaaggagtaa gccgcaaaaa
      241 gcccaaagga gaactccaag agacgtgctt tctagagggg aaaagagtgc aagaaggatg
      301 ccggtaaaaa acagcccgaa cggtaccatg tgtttggctc cgatcctgtc tcccaggaca
      361 ccgttaacaa gttggccggc gccataggtg aggaagaatg cagaacccat ggttccaaga
      421 tcgcctcttg taaagtgcgt ctcggcgatc atctggggca tgatggcgct gaggatgctc
      481 cttccggcgt agcatgacgt ataggctaaa aagcagcttg tggcaaggag ccaggtgtta
      541 agacctacct tgcctttttt aaggtgcctg ttcataggag aatcggctgg ctccaggaga
      601 cgctgccgga gaagtcttct gcagatacct ttacgaactt agttccttcg gggagctggt
      661 attctgcgta agtgataggg gagtctttgg ttccctgaaa cgacttagtt ccgtatccgc
      721 cgaatatgat gggatgggat ttgatccccg caaaaaagca cacgcttctg gtgggagtgc
      781 accagagctt gactaagccg tcttcaacaa gaaaatccag gatctccggt gccgcttcgc
      841 cttcccgggc agcggaggag tagaagcttc ccttggagat cgcatccaga atgcccttat
      901 gggagaagtc acaggtcttt acggtgatgt atccgccgca gtagtcgcta gaggcggtgg
      961 ctccgtgggc atcgtctgta gcgaagttcc agatgtagtt gccgtgccag aggacgtggt
     1021 cgtagaaaac ctccgcaaag ccgcaccgcc actcaacctg gcagccgtgg ttgtagattt
     1081 ccatgccgac gatgttcttg agatccacaa tgtccccaat ttcgtctcta gaccagtggg
     1141 gatggcagta gatcaccatg ttgttgtatt cccgcagata gtctacaagc tcttgggttg
     1201 ttagtccgtt gcagaagtgt tgatccagct tgtggccgtt tgcaaacttc atggttttcg
     1261 ggtctgcaaa ggccactacg tggtgaatac ccgatccgcc tttggaagtg tgggcttcca
     1321 cagcaggaat ggaaaggaag ttctcttctt ctgcttctgg atagtagcta aaaacgttgt
     1381 ggtcggagat gcccaggaag ctgtagccgt ttttcttgta gcgatcgatg gtctcaagag
     1441 gggagagttt tccgtctgag accgttgtgt gggtgtggag attgcctttg tagaagctac
     1501 cttctctaaa aagcgagtac tttttcaccg tcagcactcc ttttcatgtt gtggtgttta
     1561 gcaggttgac ttttcaaaac cagacaccgg attgaaaaaa aaagagatgg tgaggtaaaa
     1621 ggaatttgca ggaatcccta atctctgcac agctttgggg acccaattgc aaaggacaca
     1681 agaggggctt taggcagaac ttggaagtct acagaggagg ccgcagaggc tgaaccaaaa
     1741 aggtgcggcg tgaagatatc cctttacctc acatctctta tttaagaagc tgaaatggcc
     1801 tcgtactaaa attgattata gcatagaggc atgcctttgc aataagcagc agcaaagggc
     1861 cgtgcttttt tccattcaaa aaacccctgt gcagcttcac aggggctttc gttctaatgg
     1921 gcctttactt cggtccagat gcgatcgtat tcttttaagg ttgcaccgag attgacgaac
     1981 acttcggcgt tcaccaaatc ttcatctgta ggatagaagt cggggtcatc ggtgatctct
     2041 ttaggcagct ggtctttggc tgcaagatgc ggtgtagcgt aaccgatgtg gtccacattt
     2101 ctgaaggcga tgtccggacg gttcaagaag tcgatgaact tgtgggcagc atcgatgttc
     2161 tttgcacctt tcggaatagc cactacgtca aaccagaggt tggtgccggt tctcgggaag
     2221 gtgtaggcaa ggtcggggtt ttcttcgatg gcatatgccg catcgccaga ccaaaccact
     2281 gccagtgccg cttccccaga aaccatttta tccttacctt cgtccacaac gtaggcaagc
     2341 accaaaggtt tctgct
//
LOCUS       DAOSDA010000294         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_29836_length_2343_cov_2.916521, whole genome shotgun sequence.
ACCESSION   DAOSDA010000294 DAOSDA010000000
VERSION     DAOSDA010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29836_length_2343_cov_2.916521"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            542..1450
                     /locus_tag="PLW82_06905"
     CDS             542..1450
                     /locus_tag="PLW82_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOJ47041.1"
                     /translation="MITIDLHCDLLWYLSEKGIEAFTEASEAQITKESWERGGPDLLV
                     MAIYVDERHIPHQKEAQALKMAGIYWNLAATGCYTPVLQRKDLDMPGKKALLSIEGGA
                     PLESEEDLYAFRALGVRMLSLCWNYQNHLATGCLEQYSGGLTLKGETLLKKAQDLGIL
                     LDVSHLSEGSFWDAVKVSRLPLIASHSNSYALKPHPRNLKDDAIKEIAKLGGVIGVNF
                     CPGFLQNPSAPGVVEQVVYMAELVGPEHVGIGSDFLGIDNTVQGLEHIGRWMDLEHLL
                     EKAGFAKAEREGILGGNFKRVLSEVL"
     gene            1542..1994
                     /gene="spoVAC"
                     /locus_tag="PLW82_06910"
     CDS             1542..1994
                     /gene="spoVAC"
                     /locus_tag="PLW82_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HOJ47042.1"
                     /translation="MLRERESPQAKRYARLVEQEKPRVDVGGNVLKAFVVGGTICLIG
                     EVIKTYLMSFGLSGEVAGVWTSIALVFLSALLTGLGIYDEIARFGGAGSAIPITGFSN
                     SVVAPAMEFRSEGAILGLAARMFVIAGPVIVYGLVSATVFGLIRSVLF"
     gene            1991..>2343
                     /locus_tag="PLW82_06915"
     CDS             1991..>2343
                     /locus_tag="PLW82_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HOJ47043.1"
                     /translation="MNKTFTLKNVYVEETASVVGPTEGAGPLGDLYDVVIPDEIYGES
                     SWEKAERKMLIESIGLCMNKAGYEPADFDMILAGDLSGQLISANFAARDLPIPFAGIY
                     GACSTFTLTVGMGALL"
BASE COUNT          577 a          558 c          669 g          539 t
ORIGIN      
        1 aggatacgcc cgttaccctt gcggtagtgg aagatggctt gtacttgaaa gtggttcaag
       61 atacctgcat cttctgcagc agtaaggaga atctcttcat cgagttcaaa gaaaagaagg
      121 tgtgccggga ctgctacaac cagctttggg gcagcgagaa gtagtccgga cttggattca
      181 aatcaaagag gcagaacact cattaggcac gtcctgcaaa tacacttgtg agaagccttt
      241 tacgcgacgc agagtggatc tactaaactc cttgcaggta ccaagaggct gcagaaagaa
      301 acctcagtgg gtatctttca gcccttgcac aagtgcattt gcagagagac cgaccggaga
      361 tagatcttgc cgactgaaac aggcaagagg cgcattttct gtctgctttg ctagatagtt
      421 ctcggcttgc tgcaaaaagc gccggtagtg gcagtttctt gcacctgcga agcgatgcgt
      481 tttcgggcta gtctcctgca gaagtttgca aaaggtggtg aacccggttt aaaccggttc
      541 tatgattaca attgacctgc actgcgattt actctggtac ctaagcgaaa agggcataga
      601 agcttttacc gaagcttctg aggctcagat caccaaagaa agctgggaga ggggtggtcc
      661 cgaccttctg gtcatggcca tttacgtaga cgaaaggcac atcccccacc aaaaagaggc
      721 ccaagctctg aagatggcag ggatctactg gaaccttgct gcaacgggct gctatacacc
      781 ggtgctgcag agaaaagacc tggatatgcc cgggaagaaa gccctgctct caatcgaagg
      841 cggagcacca ctggagtcag aagaagacct ctacgctttc cgcgctctag gagtcaggat
      901 gctctctctc tgctggaact accagaacca tctggcaaca ggctgcctgg aacagtacag
      961 cggagggctt acattgaaag gcgaaaccct gcttaaaaaa gcgcaggatc tgggcatact
     1021 actagatgtg tctcacctta gcgaggggag cttttgggac gcggttaagg tctccagact
     1081 acctcttatc gccagccatt ccaacagcta cgcccttaaa ccccacccgc gaaatctcaa
     1141 agatgatgcc atcaaggaga tcgccaaatt gggcggggtg atcggcgtga acttctgtcc
     1201 cggttttctg cagaacccaa gtgcccctgg ggtggtagag caggtcgtct acatggcaga
     1261 gctggtgggt cccgaacacg tagggatcgg ctccgatttt ctgggcatcg acaacacggt
     1321 ccagggctta gagcatatcg gtagatggat ggatctggag cacctgctcg agaaagcggg
     1381 ttttgccaaa gcggaacggg aaggcatttt ggggggtaac tttaaaaggg ttttgtcaga
     1441 ggttttgtaa cagatcaggt gaagtacaaa gacagcacag ccaaaacagg ctgtgctatt
     1501 tttttgcata agtggcaata ctacccaaag gaggtggaac tatgctgaga gaacgagaaa
     1561 gcccgcaggc caagcggtac gcacgactgg ttgaacagga aaagcccagg gtcgatgtgg
     1621 ggggcaatgt cctcaaggcc tttgttgttg gcggtaccat ctgcctgatc ggcgaagtga
     1681 ttaagaccta tctgatgagt tttggcttgt ccggtgaggt agccggagtc tggacatcta
     1741 ttgctttagt ctttttgagc gctctcctta caggtctcgg catctacgac gaaatcgcca
     1801 ggtttggcgg cgccggaagt gccattccca ttacgggctt ttccaactct gttgtggccc
     1861 cggctatgga gtttcggagc gaaggagcca tcctgggcct tgcagcgcgg atgtttgtta
     1921 ttgcagggcc ggttatcgtc tacggtttgg tgtctgcaac ggtttttggg ctgataagga
     1981 gtgttctgtt ttgaacaaga cctttacttt gaaaaacgtc tatgtagaag aaacagcaag
     2041 cgtggttggt cctacagaag gtgcaggacc gctgggagac ctctacgatg tggtgattcc
     2101 cgacgagatc tacggcgaaa gctcctggga gaaagcggaa cggaagatgc tgatcgaaag
     2161 catcggcctc tgcatgaaca aagccggcta cgagcctgcg gactttgaca tgattctagc
     2221 aggcgacctt tcggggcagc tgattagtgc caacttcgcc gctagagacc ttcccatacc
     2281 ttttgcgggg atctacggag cctgctcgac ctttaccctc actgtaggga tgggcgctct
     2341 gct
//
LOCUS       DAOSDA010000295         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_29889_length_2340_cov_3.518600, whole genome shotgun sequence.
ACCESSION   DAOSDA010000295 DAOSDA010000000
VERSION     DAOSDA010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29889_length_2340_cov_3.518600"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLW82_06920"
     CDS             complement(<1..897)
                     /locus_tag="PLW82_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ47044.1"
                     /translation="MEKQLELEKRYLFTNKDLRRLILPLILEQLLAVLVGMADTVMVA
                     SVGESAVSGVSLVDSINILLINIFAALATGGAVVAGQYLGRKDQEKAVEAGEQLLVFV
                     VFAALLIMTLGYLGKNFILDVLFGKIAPDVRMHANIYLLIVFASIPFIALYNSGAALF
                     RTMGNSQVTLKISVLMNVINVVGNAVLIYGFGLGAAGAAASTLLSRAVGAVIITILLT
                     NEDLLIHIRKPFKYKFNKDLVRKILFLGIPNGVENSMFQLGKILLLSVVSGFGTASIT
                     ANAVGNSVTAFGILPGAAMGLAL"
     gene            1011..2135
                     /locus_tag="PLW82_06925"
     CDS             1011..2135
                     /locus_tag="PLW82_06925"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ47045.1"
                     /translation="MEEHPVEEGDRMIAKYLKAVLAFLAGFVFWLSLMLMVFAKNLSA
                     LSRSAAVYAEFFTKLTVGTLLLLAAWLIVTFARRSSKDLKQAALMTAASGVIAYIAMG
                     FLVFIYQDNLLFSPGSFNDSRREVISRTFPNAEEFWVTAADGTRLQGWIVKNATLEKT
                     PLAIFFAGQGSEASRYLELANRLEGFSAAFVNYRGYGQSGGKPSDKAIFADATAVYDY
                     LVSRGDVDPERIVAIGGSLGTGVATYLASQRDLMGIVLFSPYDRIAGGVTQDMLPFLP
                     TALLFRNKFDVRKYAEATDAFVLAIVGDKDRVITPWRSMLQTANFRNSPSVWTVFIDG
                     GDHYSIYEATETWTAVNNFLSFLLEHSYESSAEPPIFMML"
BASE COUNT          595 a          600 c          571 g          574 t
ORIGIN      
        1 caaagcaagg cccattgctg ctcccggcaa aatgccaaaa gcggtaactg agttacctac
       61 ggcgttagcg gtgatggagg ctgtgccaaa tcccgagacc acgctgagaa ggaggatttt
      121 ccccagctgg aacatgctgt tttccacacc gttgggaatc ccgagaaaga ggatcttccg
      181 caccaagtcc ttgttgaact tgtacttgaa aggctttctg atgtggatca gtagatcttc
      241 gttggtgagc aggatggtaa tgatcactgc accaactgcc ctggaaagca gagtcgatgc
      301 tgcagcacct gctgccccaa gcccaaagcc gtaaatgaga actgcgtttc ccacaacatt
      361 gatgacgttc atgagaaccg agatcttcaa agtgacctgg gagttgccca tagtgcggaa
      421 cagagcggct ccgctgttgt aaagggcgat aaaaggaata ctggcaaaga cgattaaaag
      481 ataaatatta gcatgcatgc gcacatcagg cgcaattttc ccgaaaagta cgtccaggat
      541 gaagttcttg cccaagtacc ccaaggtcat gataagaaga gctgcaaaca ccacaaacac
      601 caggagctgc tctcctgctt ccaccgcttt ctcctgatcc ttccgcccta ggtactgccc
      661 ggctaccacc gcacctcctg tggcaagggc tgcaaagata ttgatcagga ggatgtttat
      721 agagtccaca agggacactc cggacacagc actctcaccg acacttgcca ccattaccgt
      781 gtccgccatt cccacaagaa ctgctaagag ctgctccaag atcaggggaa ggattaagcg
      841 cctcaggtct ttgttggtaa agagatatct tttttccagt tccagttgtt tttccatata
      901 gttctacctc tacggctgcg cgaaataaga aaagccccgt atgcagaaac aaggggcgct
      961 gcgttcaata atacatatta tattaacctt tcctaagaga agtcaaacaa atggaagaac
     1021 atccagtcga agaaggtgat aggatgattg caaagtactt aaaggcagtt cttgccttcc
     1081 tcgcgggctt tgtcttctgg ctcagcttga tgctaatggt attcgcgaaa aacctttctg
     1141 ccctctctag gtctgcggcg gtctatgccg agtttttcac taagctgact gtagggactc
     1201 tgctacttct tgcagcttgg ctgatcgtca ctttcgcaag gagatcctcg aaggatctaa
     1261 agcaggcagc attaatgaca gcggcttccg gcgttattgc ctacatcgca atgggctttc
     1321 tggttttcat ctatcaagac aacctgctat ttagtcctgg ctccttcaac gactcgaggc
     1381 gcgaggtgat cagccgcaca ttccctaatg ctgaggagtt ctgggtgact gcagctgatg
     1441 gcacaaggct gcagggttgg atcgtaaaaa atgcgacttt agaaaaaacg cctcttgcca
     1501 tctttttcgc tggtcaggga agcgaggcct ccaggtacct agaactggca aacaggctcg
     1561 aaggtttttc ggcggctttc gttaactaca gaggctacgg tcaaagcggc ggaaaaccca
     1621 gcgataaagc tatatttgca gatgccacag cagtctatga ctacctggtc tccaggggag
     1681 atgtcgatcc cgagaggatc gttgccatcg gcggtagcct cggaaccggt gttgccacct
     1741 atctagcctc ccaaagagac ttaatgggca ttgtgctgtt ctcgccgtac gaccgcattg
     1801 caggtggcgt gactcaagac atgctgccat ttctgcctac agcactgctc tttcgaaaca
     1861 agtttgacgt acgaaagtac gctgaagcaa ctgacgcttt tgtccttgct atcgtcggcg
     1921 acaaagaccg agtgatcacc ccttggaggt ctatgctcca gacagcaaac ttcagaaaca
     1981 gtccctcagt gtggacggtt ttcattgatg gcggggatca ctactccatc tacgaagcca
     2041 cagaaacctg gactgcggtg aacaacttcc tttcctttct gctagaacac tcctatgaaa
     2101 gttcggcaga acctccgatt ttcatgatgc tctaacaaaa atgagtctaa attgcagaag
     2161 ctctgcccga gtgcagagct tctttacgct ttccttagcc atgaaccctc cagatctatg
     2221 gcgcctactc ttcataggtt ttcaaacgcc gggagtatta ggtgctttct acccctcaag
     2281 ctgctttggg gctgtggcgt tgatgtagaa ggctgcaaac gctgcttcta tgtagggata
//
LOCUS       DAOSDA010000296         2307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_30515_length_2307_cov_3.222025, whole genome shotgun sequence.
ACCESSION   DAOSDA010000296 DAOSDA010000000
VERSION     DAOSDA010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30515_length_2307_cov_3.222025"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLW82_06930"
     CDS             <1..768
                     /locus_tag="PLW82_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47046.1"
                     /translation="TYNLDPNLRLSAVADLKAQLAFGVPLLAKVFRFKDVSLGVAYHI
                     IPVGVYVVADSNVTLEAIYTEDDAEIDTQLSGDLYVSQSVRGSALDVGLMMRPTENLY
                     LGVAIDGLQGKLTWNNFRWIDLRDLEDQDLEDIDWAKAGTPVAGEVTQKLPVTIKLGA
                     RYDLRRWLKFYGEARHITLANDDSYFTVSGGTDITLLWLLPLKASVTYDARYGRVGYN
                     FGAGLKLLIVEANVEAYTKPHQSGDEIGLKIGARVAF"
     gene            complement(843..2132)
                     /locus_tag="PLW82_06935"
     CDS             complement(843..2132)
                     /locus_tag="PLW82_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ47047.1"
                     /translation="MLKQWLTVLAAVLCLLSGIVGAAAKPVQVWSIFSVEQTRIIQEL
                     TDAIFTPNTGIHVEITAIPSSGYEQKYLLAAISNEMPEVAVSGSLGPADMGIRGAVVD
                     LRARYGEDYEKVHQSMYPGLMRSFDFLGSAFGLPTQTNIYPMIVRTDIMNELGLQVPS
                     TWNELYEILPKLQSQGKNFATTFGIGTSVYADASMFIWQRGVDLYTPDRQRSALDTPE
                     AIAAFTEFTELFTKHKIPKEINAYMDFTTGAIPIMLTSVWNYSALVQGAPDLEGKWTL
                     SLVPGTVSADGATNHAAYIGGNSLMLFKNASRADDGWEWMKWFVSRETQIALAQLTPQ
                     RISGSMFLPSNHEAMMHVPFPEEHVEVFMEQAHQSVAPVFALIPASVIHRLFNFATNK
                     AVLQGVDPEVAIREAADEMNAELARKQKEFERFISKL"
BASE COUNT          571 a          597 c          590 g          549 t
ORIGIN      
        1 acctacaacc tagatccgaa ccttcgtctg agtgccgttg ccgatctaaa ggcccagctc
       61 gcctttggcg taccgcttct ggcgaaagtc ttcaggttca aagacgtgtc cttgggtgtc
      121 gcctaccaca tcattccggt tggcgtatac gtcgttgccg atagcaacgt caccttagaa
      181 gcaatctaca ctgaagacga cgcagaaatt gacacgcagc tttccggaga cctgtatgtt
      241 tcccagtcgg taagaggttc ggcactagat gtcggtctga tgatgaggcc aactgaaaac
      301 ctctacctag gagttgccat cgacggactc caaggcaagc tgacttggaa caacttccgt
      361 tggatagatc tgagagactt ggaagaccag gatctcgagg atatcgactg ggcaaaggcc
      421 ggaacgcctg tagctggtga agtgacccag aagttaccgg taaccatcaa gctaggtgca
      481 agatacgatc tgcgccgctg gctcaagttc tacggcgaag ccagacacat caccctggct
      541 aacgacgact cctacttcac cgtttccgga ggaaccgata tcactctcct gtggctcctg
      601 cctctcaagg cttcagttac ctacgacgca agatacggtc gtgtaggcta caacttcggc
      661 gccgggttga agttactcat cgtcgaagcg aacgttgaag cctataccaa accccaccag
      721 tcaggagacg agatcgggtt aaagatcgga gcaagagtag ctttctaaaa cccaattcaa
      781 aagcacgcaa aaatggccgc ccattgggcg gcctctttat ttctcattca cttctcttga
      841 acctacagtt tgctgataaa cctttcaaac tccttctgct ttctggcaag ctctgcgttc
      901 atttcgtctg cagcttctct gattgcaact tccggatcaa cgccctggag cactgccttg
      961 tttgtggcaa aattgaacag tctgtggatg acagaggcag gtatcaaagc aaacacagga
     1021 gcgaccgact ggtgcgcctg ctccatgaaa acctccacat gttcttcggg aaacggcacg
     1081 tgcatcatgg cttcgtggtt ggaaggcaaa aacatactgc cgctgatcct ttgaggcgtg
     1141 agctgagcta gagcgatctg ggtttctctt gaaacgaacc acttcatcca ctcccagccg
     1201 tcgtctgcac ggctggcgtt tttaaacagc atcagcgagt tgcctccgat gtaagcggca
     1261 tggttcgtgg ctccgtctgc gctaacggtc ccggggacca gagacagagt ccatttgccc
     1321 tccaaatccg gcgcgccctg taccaaggca gagtagttcc agacggaggt gagcatgatg
     1381 ggaatagcgc cagtggtaaa atccatgtag gcgttaatct ctttaggaat tttgtgtttg
     1441 gtgaagagtt ccgtaaactc ggtaaaagcc gcaatggcct ctggcgtgtc cagagccgat
     1501 ctttgacggt cgggggtgta gaggtcaacg ccccgctgcc agatgaacat agaagcgtcg
     1561 gcgtacacag aggtgccgat gccaaaagtc gtggcaaagt tctttccctg ggactgcagc
     1621 ttgggtagga tttcatagag ctcgttccag gtgcttggca cttgaagccc cagttcgttc
     1681 atgatgtctg ttctgacgat catgggatag atgttggttt gggtcggcaa cccgaaggcg
     1741 ctgccaagga aatcaaaaga acgcataagg ccaggataca tgctctgatg gaccttttcg
     1801 tagtcttcgc catagcgtgc gcgtagatct actactgcac ctctgatgcc catatcggca
     1861 ggaccgagag agccgcttac ggcaacttca ggcatctcgt tggaaattgc agcaaggaga
     1921 tacttctgct cgtagcctga agagggaata gctgtgatct ctacatgaat gccagtgttt
     1981 ggcgtgaaaa tcgcgtcggt aagttcctgg ataatcctag tctgctcaac tgaaaagatg
     2041 gaccagactt gaacaggttt agccgctgct cctacaatgc cggacaaaag acacaaaact
     2101 gctgcgagaa ctgtcaacca ttgtttcagc aacctatgac ccccctttta gttagtggtg
     2161 ctcataccca caacttccgt atgacgagcg ctattccttt attcttgaag tggaaatgta
     2221 ttgatgcacg ccaaacagat caccaaacca atgggatcaa gtccataagt gtgtgtaaaa
     2281 tccctcggtt agatgaactc agcgtat
//
LOCUS       DAOSDA010000297         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_30545_length_2305_cov_3.850667, whole genome shotgun sequence.
ACCESSION   DAOSDA010000297 DAOSDA010000000
VERSION     DAOSDA010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30545_length_2305_cov_3.850667"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..647
                     /gene="tsaE"
                     /locus_tag="PLW82_06940"
     CDS             177..647
                     /gene="tsaE"
                     /locus_tag="PLW82_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOJ47048.1"
                     /translation="MIFKLKSEEDTHKAGQILGEYASPGDLFCLIGTLGAGKTYLTKG
                     IAKSLGIDPEKVTSPTFTLVQEHMGRMPLYHWDLYRLDDPKEALELGLEEHLYGDGLT
                     VIEWAELLDAELPKERLTIKLNIENGFRTIEIVPTKSYEKVYGEIKERFMSCFA"
     gene            635..1321
                     /gene="tsaB"
                     /locus_tag="PLW82_06945"
     CDS             635..1321
                     /gene="tsaB"
                     /locus_tag="PLW82_06945"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOJ47049.1"
                     /translation="MLCLALDTSTKTGGAALVKGDSILGEFVLDIERKTHSERVIPAI
                     DSLLQGSGITMQEIEALAIAYGPGSFTGLRIGVGTVKALSLSLNIPVYKVDTLEALAS
                     QIGFVDGIICPIFNARRGEVYGAVWESRPKQDIFACVMESRALSIDELLLNVPKGDNL
                     FFVGEGAISERDSILQTFPHATIVTGTNSMLHPGSVGLRALKAGTPVDADSLLPNYLR
                     KSEAEVKRGL"
     gene            1323..1823
                     /gene="rimI"
                     /locus_tag="PLW82_06950"
     CDS             1323..1823
                     /gene="rimI"
                     /locus_tag="PLW82_06950"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOJ47050.1"
                     /translation="MGQYEDWKTSNVPAESGKAAFTIRRAVFEDLEGIARVERESFTT
                     PWTETSIGTELSLNIFARYFVAELEGEIVGFGGMWVVVDEAHITNIAVLEKHRRQGIG
                     RAILHTLLSTAKEERCRAATLEVRVGNIQARRLYESFGFAPVGIRPKYYSDTNEDALI
                     MWCVLE"
     gene            1938..>2305
                     /locus_tag="PLW82_06955"
     CDS             1938..>2305
                     /locus_tag="PLW82_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOJ47051.1"
                     /translation="MSALVLGIETSCDETAASVVDAGREILSNVIATQIPWHVEFGGV
                     VPEIASRKHLEFINPVIKKALSDAGVGLEDIAAVAVTYGPGLVGGLLVGLSAAKAIAY
                     SRNLPLIGVNHIEGHIYANFL"
BASE COUNT          618 a          502 c          619 g          566 t
ORIGIN      
        1 actggttgat caatatggtt atacttggta tagcatcatt tgtctttctt ctagacctcg
       61 tcgtctggag gagattcagt cttagcttga tgtggatgat catcaccggt ggcgtagctg
      121 ttttctgcta cgtgcaattg aaaagcatcg acgatagtgc tgaagggaag aaatagatga
      181 ttttcaaact caaaagcgaa gaggatacgc acaaagcagg acaaatcttg ggagagtatg
      241 cctctcctgg agatttgttt tgcctaatag ggacgttagg tgctggaaag acttacctca
      301 ccaaaggtat tgccaaaagc cttggcattg acccggaaaa ggtcaccagc cctaccttta
      361 cgttggttca ggaacacatg ggcaggatgc ccctctacca ttgggacctc tacaggctcg
      421 acgatcccaa agaagcgttg gagttaggac ttgaggagca cctctacgga gacggtctta
      481 cggtcataga gtgggcagaa cttttagatg cagaactccc caaggagagg ctgaccataa
      541 aactcaacat tgaaaacggt ttccgaacca ttgagatcgt acccaccaag tcctacgaga
      601 aggtttatgg agagattaag gagagattca tgtcatgctt tgcttagcgc tggatacctc
      661 cacgaaaacc ggcggtgcag ctctggtaaa aggagacagc atcctgggag agttcgtttt
      721 ggatattgag agaaaaaccc attcagaacg ggttatacct gcaattgaca gcctgctcca
      781 gggctccggt atcaccatgc aggagattga agcactggcc atcgcttatg gtcccggatc
      841 ttttacgggc ctcaggattg gagtaggcac tgtcaaagca ctatctctaa gcttaaacat
      901 cccggtgtat aaagtagaca ccttagaggc acttgccagc cagattggtt ttgttgatgg
      961 aatcatctgc cccattttca acgccaggcg cggcgaggtc tacggggctg tatgggagag
     1021 cagacctaaa caggacattt tcgcttgcgt catggagtcc cgagctcttt ccattgatga
     1081 gcttctttta aatgtgccaa aaggggacaa cctctttttt gttggtgaag gagccatcag
     1141 cgaaagagat tctatccttc agacgtttcc ccacgctacg atagtaacgg gaacaaacag
     1201 catgcttcat cctgggtccg tcggccttag agctctgaaa gccggaactc cagttgatgc
     1261 cgacagcttg ctcccaaact acctcagaaa atcggaggcc gaggtcaaaa gagggttgta
     1321 atatgggtca gtatgaagat tggaaaacaa gcaatgtgcc tgcagaaagc ggcaaagccg
     1381 cctttaccat ccgccgggca gtgttcgagg atttggaagg aattgccaga gtggagaggg
     1441 agtcctttac cactccctgg accgagacat ctataggaac tgaactgtcg ttaaacatct
     1501 ttgcaagata cttcgtggca gagttggagg gcgaaatcgt cgggttcggc ggaatgtggg
     1561 ttgtggtcga tgaagcccat atcacaaaca ttgccgtact ggaaaaacat cgcagacagg
     1621 gaataggcag ggccattcta catacactgc tttccactgc caaggaagag cggtgcagag
     1681 cagcgactct cgaggtcaga gtgggaaata tccaggcacg cagactatat gagagtttcg
     1741 gctttgcccc tgtgggtata agacctaagt actacagcga tacaaatgaa gatgcactca
     1801 ttatgtggtg tgtgttagaa tagagcggaa accccgcttt ttttctatga aatggagatc
     1861 agaaagagtg atcgttgatt ctccaatttg cacaagctaa gaaagcgaag ttaaccgagg
     1921 cgttttgagg tgaatatatg tctgcattag tgttaggtat agagactagc tgcgatgaaa
     1981 ccgcagctag cgtagtagat gctggaagag agattctgtc taatgttatt gcaacccaga
     2041 taccctggca cgtagagttc ggaggcgttg tgcctgagat tgcgtccagg aagcatttgg
     2101 agttcatcaa tcctgtgatc aagaaggcct taagcgatgc tggtgtgggt ttggaggaca
     2161 ttgcagctgt tgcagtaacc tacggacctg gccttgttgg cgggctgctt gtaggtcttt
     2221 cagcagcaaa ggccattgcc tatagcagga atctgccctt aatcggggtt aaccatatcg
     2281 aggggcatat ctacgctaac ttcct
//
LOCUS       DAOSDA010000298         2290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_30835_length_2290_cov_3.165548, whole genome shotgun sequence.
ACCESSION   DAOSDA010000298 DAOSDA010000000
VERSION     DAOSDA010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30835_length_2290_cov_3.165548"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLW82_06960"
     CDS             complement(<1..358)
                     /locus_tag="PLW82_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOJ47052.1"
                     /translation="MVKKLVIAVLVLILILGGAYALLYTFMSRELEKLGEIAKIDLSL
                     IEDGVYEGYSRAGLVAAELRVVVKNHRIEQIEIIDHVYGTGKPAEAVLDAVVEKQSLQ
                     VDAVAGSTASSKNLLKA"
     gene            complement(399..866)
                     /locus_tag="PLW82_06965"
     CDS             complement(399..866)
                     /locus_tag="PLW82_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOJ47053.1"
                     /translation="MQLVVYSSKHGSTKRCAEMLAEMLQADLLNAAEGLEVSLDKYDV
                     VVLGSPVYVGQLLKPMKRFLEEHRTALQEKKLGLFVLCGFEERAMEIMEQTFPKELTS
                     IAACKGYFGYSYTNLPFFERLLCRMIGADVGTEKLLTEEIAAFAEKLKQEAVR"
     gene            1062..>2290
                     /locus_tag="PLW82_06970"
     CDS             1062..>2290
                     /locus_tag="PLW82_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ47054.1"
                     /translation="MRVLAFILIAFLLVSPLQALAAGGEAIAVLEQKVLDAKRALKDL
                     DYETIDRLLQDLKEGRGTEETTGLIDDLLIESLAVETRAIWLDSVHLNTLTSREKIAE
                     AMDLLASINVNVILVDIFGNGHSVFPSKVAEQRDAFRFLAEGDIVPILIEEAHLRGIE
                     FHALASLFGVDSYGIGPFMDDRLHWFDRTKDGGILTGIGDFEAFFSPVLPEVREFFLE
                     VIKEILTYDIDGLHLDYIRFGRNVGYHPYTLQLHKEAGGAPENLDDPKEMERFTEFRS
                     SFISSFVDEVRILMQETKPGLLLSAAVGSNLTDAQREICQDWHDWAERRLLHLIFHMC
                     YVETSAQYQVVIDNDPGAIKDLAISFPGIALYAVDGKELLHQLEVGQTNPVSGQALFS
                     TVHLKPELVEVLGKVWR"
BASE COUNT          559 a          566 c          602 g          563 t
ORIGIN      
        1 ccgctttgag aaggttctta ctgcttgcgg tagaacctgc aactgcatct acctgaaggg
       61 actgcttctc aactacggca tcaagtaccg cttctgcagg ctttccggta ccataaacgt
      121 ggtcaatgat ctcaatctgc tcgatcctgt ggtttttcac aaccacccga agctctgcag
      181 cgacgagccc ggctctggag tagccctcgt agacgccgtc ttcaatgaga gaaaggtcga
      241 tcttggcaat ctctcctagc ttttccagtt ctcttgacat gaacgtgtaa agcagagcgt
      301 aagctcctcc tagaatgagg atcaagacca aaacagcgat tacaagcttt ttcaccatag
      361 gcatccctcc tttgtgttta tccgctttca cctgatagct agcgcaccgc ttcctgcttt
      421 agcttttcag cgaatgctgc aatttcttca gtaaggagct tttcggttcc tacatcagcg
      481 ccgatcatgc ggcagagcag tcgctcaaag aaggggagat tggtgtagga atagccgaaa
      541 taacctttgc aggcagcaat gctagtaagc tctttgggaa aggtctgttc catgatctcc
      601 atggctcgtt cttcaaagcc gcagagcaca aacaggccca gcttcttctc ttggagggct
      661 gtgcggtgct cctcgagaaa tcgcttcatg ggctttagga gctgcccaac atagacggga
      721 cttccgagca ccaccacgtc gtacttatcg agggaaacct ccaggccttc tgcggcgttt
      781 aagagatctg cctgcagcat ctctgcaagc atttctgcac accttttggt ggaaccgtgt
      841 ttgctggaat agacaactag ttgcataaaa gcactccttt cgagatcgat tgcactagga
      901 ttaagtatgt aggtacgaga cgttcgggac agcaagactc cgttttgcag cgggaaaaaa
      961 cctgagtaaa ccgaacaaga aacaataaaa gcagaggaaa agcctgtctt tcggacgaag
     1021 ctaaggggtg ttggaaaacg cggtgatttg gggggagtta tgtgagagtt ttggcgttta
     1081 tccttatcgc ttttttactt gttagtcctc tgcaggcttt ggctgcagga ggagaagcta
     1141 tagcagttct tgagcagaaa gttcttgatg caaaaagagc actgaaagac ctggactacg
     1201 aaaccataga ccgtcttctg caggatctga aagagggaag aggcactgaa gagacgacag
     1261 gccttattga tgatctttta atcgagtccc tggcggtgga aactcgggcc atttggctgg
     1321 acagcgtcca cctgaacacc cttacctcta gagaaaaaat cgctgaggcc atggatcttc
     1381 ttgctagcat caatgtgaac gtcattctgg tagacatctt cggcaacggg cacagcgtct
     1441 ttccgagcaa agtggcagag cagagagacg cttttaggtt ccttgcagaa ggagacattg
     1501 tacccatact tattgaagaa gctcacctgc ggggcataga gttccatgct cttgccagtc
     1561 ttttcggtgt ggacagctac ggcatcggtc catttatgga cgaccggctg cactggtttg
     1621 accgcacgaa agacggagga attctcaccg gcatcggcga cttcgaggcc tttttctcgc
     1681 cggttcttcc tgaggtcagg gagtttttcc tggaagtcat caaggagatt ttgacctacg
     1741 acatcgacgg tttgcacctg gactacatcc gcttcggccg caacgtaggc taccatccgt
     1801 acactctgca actccacaaa gaggcgggag gcgctccgga aaacctggac gaccccaagg
     1861 agatggaaag gttcaccgag tttcggagtt cgtttatatc ttcgtttgtg gacgaagtac
     1921 ggatactgat gcaagagacc aaaccaggtc ttctcctttc ggctgctgta gggtccaact
     1981 tgaccgacgc ccagagggag atctgccagg actggcacga ttgggccgaa agaaggctgc
     2041 tgcacctgat cttccacatg tgctatgtgg aaaccagcgc tcagtaccag gttgtaatcg
     2101 ataacgaccc aggggcaatc aaggatcttg ccatcagctt cccggggatc gctctgtacg
     2161 ccgtagatgg aaaggaactc ttgcaccagc tggaggtagg gcaaaccaat ccggtatcag
     2221 gtcaggccct gttttccact gttcatttga aaccggaact ggtggaggtt ttgggcaagg
     2281 tttggcgtag
//
LOCUS       DAOSDA010000299         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_30948_length_2283_cov_3.355925, whole genome shotgun sequence.
ACCESSION   DAOSDA010000299 DAOSDA010000000
VERSION     DAOSDA010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30948_length_2283_cov_3.355925"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..116)
                     /locus_tag="PLW82_06975"
     tRNA            complement(31..116)
                     /locus_tag="PLW82_06975"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79..81),aa:Tyr,seq:gta)
     gene            complement(118..193)
                     /locus_tag="PLW82_06980"
     tRNA            complement(118..193)
                     /locus_tag="PLW82_06980"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(158..160),aa:Thr,seq:tgt)
     gene            complement(421..1074)
                     /gene="sigH"
                     /locus_tag="PLW82_06985"
     CDS             complement(421..1074)
                     /gene="sigH"
                     /locus_tag="PLW82_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HOJ47055.1"
                     /translation="MTSSAKKKPYAMYETVPDEEIVEYARGGSEVALEYLINKYKNFV
                     RAKARSYFLIGADREDIIQEGMIGLYKAIRDFRVEKLTSFRAFAELCITRQIITAIKT
                     ATRQKHIPLNSYVSLNKPIYDEESDRTLLDILSGTKVSDPEELVISQEEFNAIEKKMG
                     EFLSELEWQVLNYYLEGKSYQEIADELDRHVKSIDNALQRVKRKLERYLEKRESQDR"
     gene            complement(1214..1927)
                     /gene="rlmB"
                     /locus_tag="PLW82_06990"
     CDS             complement(1214..1927)
                     /gene="rlmB"
                     /locus_tag="PLW82_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOJ47056.1"
                     /translation="MARQVEGRNAVLEALRSGRARRVYLSQTAQGKVVDQIEKLARDL
                     KIPVVKEPNDVIDKMASTKNHQGALAEAFDLPKMSLEEVFELENPFVVVLDHLQDPHN
                     LGAILRTAEAAGVDAVIVPEDRSVSIGPGAVKASAGAIEYVPIITVTNIARTVDQLKK
                     ANIWVVGTSDRADQLYTDVDLKGRLAVVIGSEGTGISRLVQEKCDFLVKIPMKGKISS
                     LNASVAAGIILYEAVRQRR"
     gene            complement(1911..>2283)
                     /locus_tag="PLW82_06995"
     CDS             complement(1911..>2283)
                     /locus_tag="PLW82_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOJ47057.1"
                     /translation="AWAYLGDGLWELFVRENLMQESLKKAGDYHSIAVPFASAKTQAQ
                     IIKSVWDDLPEKEREIFRRGRNSCSSYRRNLTHQEYAYSTGFEAILGYLYLAGEKERL
                     MELLQVFFAKGKEVLNNGKTS"
BASE COUNT          562 a          580 c          490 g          651 t
ORIGIN      
        1 ctgagctact cccgctcgat ttaaagaaaa tggtggagag gactggattc gaaccagtga
       61 aggcacagcc agcagattta cagtctgccc cctttggcca gctcgggaac ctctccatgg
      121 agctggtgat gggactcgaa cccgcaacct actgattaca agtcagttgc tctgccaatt
      181 gagctacacc ggcatgtgat tgatgtgttg tttcgtaagt cgacaagatg attatatcct
      241 gcgacatagt ggatgtcaac atctttttga gaaaagatag taggaatatc agcgtttact
      301 gctgcagatg ccaggttctg cccctaaggg tttaccggga ttttattata gcatagaacc
      361 atatggggat aaagaggaaa atccaaagtt aaacaacaaa aggaagcgat tagctccctt
      421 ttatctgtct tgagactcac gtttttctaa atagcgttca agtttgcgct taactctctg
      481 taatgcatta tcgattgact tgacgtgacg gtcgagttca tctgctattt cctgatatga
      541 cttaccttcc aaataataat ttaatacctg ccattccagt tcgctaagaa actcacccat
      601 ctttttttca atcgcattaa actcttcttg gctgataacc aactcttctg gatcgcttac
      661 tttggttccg gacagtatat caagtaacgt tctatctgat tcctcatcgt aaatcggttt
      721 atttagagaa acatatgagt tcagcggaat gtgcttttgc cttgttgctg tttttatggc
      781 tgtaatgatt tggcgagtga tacacagctc agcaaaggct ctaaatgaag tcagcttttc
      841 aaccctgaaa tcgcgaatag ctttgtaaag gccgatcata ccttcttgaa taatatcctc
      901 tcggtcggca cctattaaga agtacgaacg tgctttagct ctgacgaaat tcttgtactt
      961 gttaatcaag tactccagag ctacctcact tccgcctcgc gcatactcga cgatctcctc
     1021 atcgggtacg gtctcgtaca tcgcataggg tttcttttta gctgaagagg tcacccacat
     1081 cgcctccaac aaggagtttt gtgggacatt tagatagatg catgcgactg cagctatttg
     1141 cctgtcccat gttcctttaa cattatactg attaatgcca agcaggtcaa gttatgcaca
     1201 gatcttacct tatttacctt ctttggcgga cggcttcata aaggatgatc cccgcagcaa
     1261 cagaggcatt cagcgacgaa atcttccctt tcatggggat tttaaccagg aaatcgcact
     1321 tctcctgaac caggcggctg atgccagtgc cctcgctgcc gatgacaacg gcaagccttc
     1381 ccttaagatc gacatctgtg tagagttgat ccgctctatc tgaagtaccc actacccaga
     1441 tattagcctt cttcagctgg tcaactgtcc tggcgatgtt ggtcaccgtg attattggta
     1501 cgtattcgat tgcacctgca gaagccttga cggcaccagg cccgatggaa accgaacggt
     1561 cctcaggcac gattaccgca tcgacacctg ccgcttcagc ggtccttaaa atagccccaa
     1621 gattgtgggg gtcctggaga tggtccagaa caaccacgaa aggattctcc agctcgaaga
     1681 cctcttccag gctcatctta ggtaggtcaa aagcttcggc caacgcgcct tggtggtttt
     1741 tggtgctcgc catcttgtcg atcacatcat taggttcctt gaccaccgga atcttaaggt
     1801 ctcttgccag cttttcgatc tggtcaacaa ccttgccctg agcagtttgg ctgagataga
     1861 cccgccttgc ccgccccgat cgtaaagctt ccaggacagc atttcttcct tcaacttgtc
     1921 ttgccattgt tcaagacctc ttttcctttg gcgaaaaaaa cctgcaaaag ctccatgagc
     1981 cgctctttct cacctgctag atataggtaa cccaaaatcg cttcaaaccc cgtactgtag
     2041 gcgtactctt ggtgggtcag atttcgccgg tatgagctgc acgagttcct gcctctacga
     2101 aagatctctc tttctttttc aggaagatca tcccacaccg acttgatgat ctgagcttgg
     2161 gtttttgcag aagcaaaggg aactgcaatg ctgtggtagt ctccggcttt tttaagactt
     2221 tcctgcatga ggttctcacg aacaaacagt tcccacagtc cgtcgccaag atacgcccac
     2281 gct
//
LOCUS       DAOSDA010000300         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_31369_length_2261_cov_4.276972, whole genome shotgun sequence.
ACCESSION   DAOSDA010000300 DAOSDA010000000
VERSION     DAOSDA010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31369_length_2261_cov_4.276972"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PLW82_07000"
     CDS             <1..993
                     /locus_tag="PLW82_07000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOJ47058.1"
                     /translation="KARLLPMRTLFRKFPRVTRDLATKAKKKIRFEMSGEDTEIDRSV
                     IEQISDPIIHLLRNAIDHGIEMPEERERLGKDPTGTISLIAEQEESYIVIRIKDDGKG
                     IDPEKIVATAQRKGLITRERAQNLTPKEAVELIFLPGFSTAEQVTQVSGRGVGMDIVR
                     KNIENLSGNIQIITEVGKGTEFRIELPLTLAIMRTLLFESSNTLFSIALSAVAETNRV
                     SHSEIKYVNKQKAMMLRGRILPLIHIEEIFDLPAPEMRGEHFYVIVVRRQRTQVGIIV
                     EKLLGEEEVVVKTLSKLLGDVRGLSGATILGHGEIAFILDVPVLLEYGTDGRVE"
     gene            1149..1682
                     /locus_tag="PLW82_07005"
     CDS             1149..1682
                     /locus_tag="PLW82_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOJ47059.1"
                     /translation="MRKTDKTVNLGEIGLSKTHSVLSCYGLGSCVAVALFDPETKIGG
                     LAHVVLPEDAEYNLNMGNKEAYLLLIDLLGRPAKFADSGLTVMLNKLKECGAKLHNLQ
                     AKVVGGARVLPPISLNSLELDIGKQNEIAVKRQLERYDIPIVASDLGGSESRTVKFHP
                     ADGAVEVWRNWSGKIVI"
     gene            1704..2060
                     /locus_tag="PLW82_07010"
     CDS             1704..2060
                     /locus_tag="PLW82_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ47060.1"
                     /translation="MTKVLVVDDASFMRMRMSKLLTENNFEVHEAENGLDAFNKYKEG
                     QYDLVIMDITMPVMDGIEAVQKIVEYDPEAKIIMCSALGQQQMVIEALQAGAKDFIVK
                     PFQPDRIMESIKRVLG"
     gene            2075..>2261
                     /locus_tag="PLW82_07015"
     CDS             2075..>2261
                     /locus_tag="PLW82_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ47061.1"
                     /translation="MSLSNQDRKIRVLVVDDSAFMRTIIKNLLNKDPDIEVVGVAVDG
                     YDALEKVKELQPDVVTLD"
BASE COUNT          661 a          469 c          587 g          544 t
ORIGIN      
        1 aaggcaagac ttctgcctat gagaactctg ttccgtaagt tccctcgggt tacgcgagat
       61 ctcgccacca aggccaagaa aaagatccgc tttgaaatgt ccggtgagga caccgagatt
      121 gaccgttccg ttatcgaaca gatcagcgat cctatcattc accttctgag aaatgctata
      181 gaccatggca tcgagatgcc ggaggagcgg gagaggctcg gtaaagatcc tacaggtacc
      241 atcagcctca tcgctgaaca agaagaaagc tatattgtca ttcgaatcaa agacgatggc
      301 aagggcatcg atccggaaaa aatcgtagca acggctcaaa ggaagggctt aatcactcgt
      361 gagagagcgc aaaacctcac gccgaaagaa gcagttgagc tgatcttcct tccaggtttt
      421 tccactgccg aacaggttac ccaggtctcc ggacgcggag tgggtatgga cattgtccgc
      481 aaaaacattg agaacctcag cggtaacatc cagatcatta ccgaagtcgg taagggtacg
      541 gagttcagga tcgaactgcc tttgactttg gcgattatgc ggaccctctt gtttgaaagc
      601 tccaatacgc tgttctccat tgcactgtct gctgttgctg aaacaaaccg agtttcccat
      661 tctgagatta agtatgttaa caagcaaaag gccatgatgc tcagaggaag aatcctgcca
      721 ctgattcaca tcgaagagat cttcgatctc ccggctccgg aaatgcgcgg tgagcatttc
      781 tatgtgatag tcgtaagaag gcagagaact caggttggca tcatcgttga aaagcttcta
      841 ggagaagaag aagtggttgt taagaccttg tccaaactac tcggcgatgt taggggactg
      901 tctggagcca ccattctcgg gcacggcgag atcgcattca ttctcgacgt accggtgctt
      961 ctcgagtacg ggacagatgg ccgggtagag tgaagtttga atgcaacagc aaaaaggaat
     1021 aaagtctaaa ataaagaaac atttggttaa catctttgtt tacggatcga aaaataaaaa
     1081 ggtgtctatg tttgtgcttc ccgtcagatt taggcacctt tgcacattta aggtttaagg
     1141 tgtggtttat gagaaagact gataaaactg taaacttggg ggaaatcggc cttagcaaaa
     1201 ctcacagcgt tctcagctgc tacggactag gttcttgcgt agccgttgca ctctttgacc
     1261 ccgaaaccaa gatcggcgga ttagcccacg tggttctgcc agaagacgca gagtataacc
     1321 tcaacatggg caataaagag gcttatcttc tgctgatcga tttattggga agacctgcaa
     1381 agtttgccga ttcaggtcta accgttatgc ttaataaact gaaagagtgc ggagccaagc
     1441 tccataatct ccaggccaaa gtcgtaggtg gtgccagggt gcttccaccg atttctctga
     1501 actcactaga gctggatata ggtaagcaaa acgaaattgc agttaagaga cagctggaga
     1561 ggtacgacat accgatagta gcaagcgatc tcggcggaag tgaaagcagg acggtaaagt
     1621 ttcatcctgc agacggtgct gttgaggttt ggaggaattg gtctggaaag atcgtaatat
     1681 gaacgagact tggaggagtt aaagtgacaa aagtcttagt ggttgatgat gcaagcttta
     1741 tgcgcatgag gatgagtaag ctgctcactg aaaacaactt tgaagtccat gaagcggaaa
     1801 acggcttgga tgcttttaac aagtacaagg aaggtcagta tgatctcgtg atcatggaca
     1861 tcaccatgcc agtcatggat ggaattgaag ctgtgcaaaa aatcgtcgag tacgatccag
     1921 aagctaagat cattatgtgc agcgcgttag gacagcagca gatggtaatt gaagctcttc
     1981 aagcaggggc caaggatttc attgtgaagc catttcaacc agaccggatc atggagtcca
     2041 ttaaacgcgt tctaggttaa gaaggagatc ttaaatgtca ctttcaaatc aagatagaaa
     2101 aatcagggtt ttggtcgtag atgattcggc ctttatgcgc accataatca agaacttgct
     2161 taacaaagat ccggatatcg aagttgtcgg agttgcagtg gatggttacg atgcactgga
     2221 gaaggttaaa gagctgcagc ccgatgttgt caccttggac g
//
LOCUS       DAOSDA010000301         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_31443_length_2257_cov_3.371480, whole genome shotgun sequence.
ACCESSION   DAOSDA010000301 DAOSDA010000000
VERSION     DAOSDA010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31443_length_2257_cov_3.371480"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1018
                     /locus_tag="PLW82_07020"
     CDS             203..1018
                     /locus_tag="PLW82_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ47062.1"
                     /translation="MHLTEIFIDGFRSFKQPTTIDLTDMYTGLTNNRIIFVGTNASGK
                     TTLLETVVSLLKAWHGLEDDLYRSLVEEARSVSIGFRGSDSSAALRLEQGKLHKEGTA
                     ELPYFIYFPGNRSFFPEEEVEKLDEDWGYTYSFEQGLLHRELEDGFGQLSAEFFAGRT
                     LARSDRGYTLSSAADQSRAIPLERIPAGEKQALILLTIIDKYLRPGSIVLIDEFEVSL
                     HPTAQRRLFHLLSEKLKRNGSQLVLATHSVEIIRLANEAEVFSLDEICGGACQ"
     gene            1015..1869
                     /locus_tag="PLW82_07025"
     CDS             1015..1869
                     /locus_tag="PLW82_07025"
                     /inference="COORDINATES: protein motif:HMM:NF025844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4435 domain-containing protein"
                     /protein_id="HOJ47063.1"
                     /translation="MNYLSLDQLLLKIRSGNGLGVIIEGVTDGDDEWVYSQWFGHLAH
                     LITFYPQDGYSRVYDAVAKLNYSTKKQLVVGIIDRDFATDLELKAQFEPGYCDHVYRT
                     KRYTLENYLLDPEAWFHIAKLFWRNNLPEGFRSVEECTETINKIYEAFLPAAAYNWMA
                     HDISRQYKPKKHLEYKTMPNLDPEALYLNLLIYCRNEDLPDPEEFTIRLDILKNNRQM
                     WDQKVNGKMVFDYLLESFPVPRQKPNRHLFVNYYVSSTEAPEELVRLVEFLIKKYLGE
                     ELLTKSAV"
     gene            complement(1823..>2257)
                     /locus_tag="PLW82_07030"
     CDS             complement(1823..>2257)
                     /locus_tag="PLW82_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47064.1"
                     /translation="GISRFFDRGLDRCGLKIEENADLAVGEEIIYKPHLGVGDGLWLG
                     LLLGEITAVMGRPLSEIIAEMEEVVGRTETRLLLLEKGELEDYLRKISGKKYSLEERG
                     ELQLAGTTIAWRKQGANLYQVLLDGEPSKLRSLSEVLPPGIS"
BASE COUNT          614 a          581 c          520 g          542 t
ORIGIN      
        1 atggccccct cttttttcag gaaaccaatt ttacaccatt atacggactt aacttggcgg
       61 tgtaaacaat caagaaaaaa ggaatctcct aggttgtaga gaatgattcc cattagagta
      121 caaaacattg catgcatctt gagtccgcgc acctgaacaa ggtgcctttt taaacatctc
      181 tttaagcaaa ggaggagtca cattgcatct caccgagatt ttcatagacg gtttcagatc
      241 gtttaagcaa ccaacaacta ttgatcttac agatatgtat accggactta ccaataaccg
      301 catcattttc gtcggtacca atgcgtcggg aaagactaca ctgcttgaga cagtcgtttc
      361 actgcttaaa gcatggcacg gcttggaaga cgacctttac cgcagcttgg ttgaagaagc
      421 gcgcagcgta tcgatcgggt ttaggggaag cgattcctca gctgcgctcc gtctagagca
      481 gggaaaactg cacaaggaag gcaccgccga actcccctac ttcatctact ttccgggaaa
      541 ccggtctttc ttcccagaag aagaggtgga aaaactcgac gaagactggg gctacacata
      601 ctcctttgag caaggtcttc tgcacaggga gctggaggat gggttcggac agctgtcagc
      661 agagtttttt gcgggaagga ctctagcacg aagcgaccga gggtacaccc tgtcttccgc
      721 tgctgatcag tcgcgagcga ttcctctaga aagaatccca gctggtgaaa agcaggcctt
      781 gattcttctt acaatcatcg acaagtatct cagaccaggc tcaattgtac taattgacga
      841 gtttgaggtc agcctacatc ccacagctca acgaaggctc tttcacctgt taagcgagaa
      901 actcaaaaga aatggctcgc agcttgtctt ggccactcat tctgtggaga ttatcaggct
      961 tgccaacgaa gcggaagtct tcagtctcga cgaaatctgc ggaggtgctt gccagtgaac
     1021 tacctttcat tagatcaact gcttttgaaa atccgttcag gtaatggtct gggcgtgatt
     1081 attgaaggcg taacagatgg cgacgatgaa tgggtctaca gtcagtggtt cggacacctc
     1141 gcccatctga ttaccttcta tcctcaagac ggttacagcc gtgtctacga cgcggtagcg
     1201 aaactgaact acagcacaaa aaaacagctc gtggtgggca tcatcgatcg cgattttgcc
     1261 acagacctag agctgaaagc ccagtttgaa ccgggctact gtgatcacgt ctacagaacg
     1321 aagcgctaca ctttggaaaa ctacctttta gaccctgaag cttggttcca cattgccaag
     1381 ctgttttggc gaaataacct gccagaaggg ttcagaagcg tagaagaatg caccgagacc
     1441 atcaataaga tctacgaagc gtttcttccc gccgcagcct acaactggat ggctcacgat
     1501 atcagccggc agtacaaacc caaaaagcat ctggagtaca aaaccatgcc caatctcgac
     1561 cctgaagccc tctatctgaa cctcttaatc tactgccgca acgaagacct tccagatccg
     1621 gaagagttca ccatcagact ggacatcctg aaaaacaaca ggcaaatgtg ggatcagaag
     1681 gtcaacggta aaatggtttt cgactacctg cttgagagtt tccctgtacc gagacaaaaa
     1741 cccaaccgcc acctttttgt caactactat gtatccagta cggaagctcc cgaagagctg
     1801 gtccgcctgg tggagttttt gatcaagaaa tacctggggg aagaacttct gacaaagagc
     1861 gcagtttaga aggttcgcca tcgagaagca cttgatagag atttgcaccc tgctttctcc
     1921 aggcgattgt cgttccagcc aactgtaatt ctcctcgctc ttccagagag tacttcttgc
     1981 ctgagatctt cctcaggtag tcttcaagct cacctttttc caggagtaga agtctggttt
     2041 ctgttctgcc cacaacctcc tccatttcgg caatgatctc cgacaatggc ctgcccataa
     2101 ccgcggtgat ttctccgagg agaagaccca accagagacc atcaccaaca ccgagatggg
     2161 gtttatagat gatttcctcg ccgactgcaa ggtcggcgtt ttcttctatc ttcagaccac
     2221 agcggtcgag acctctgtcg aaaaaacggg agatgcc
//
LOCUS       DAOSDA010000302         2241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_31758_length_2241_cov_2.713632, whole genome shotgun sequence.
ACCESSION   DAOSDA010000302 DAOSDA010000000
VERSION     DAOSDA010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31758_length_2241_cov_2.713632"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLW82_07035"
     CDS             <1..766
                     /locus_tag="PLW82_07035"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOJ47065.1"
                     /translation="TLEEYADQAVAVGLSEIGFSDHLPLSPAPADVLSRDWAMNEEEL
                     PEYVEDIYSLQRKYPSLPIKLGIEADYFPDRTSIAKTEKMLQAFSWDYVIGSVHHLGY
                     WTVDSSEYLHEFERRDLYKVYEDYFETVIKAANSGLFDVLGHIDLVKKFGHVPRADLT
                     SLYRDFAQEIKKSGIAFEINTSGLRKPIGEIYPAEGLLRELLNLQVPVTLGSDSHSPL
                     EVGKDFDKALELLKRYGTDRIATFTKRQRIMRPLEV"
     gene            780..>2241
                     /locus_tag="PLW82_07040"
     CDS             780..>2241
                     /locus_tag="PLW82_07040"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ47066.1"
                     /translation="MLDVQAFLREITSMEEYKNQITAVKTIPAEKPVYGDADLGEFLE
                     SLLKRVGIEKLYSHQATALEKARRGENLVVVSGTASGKTLCYNLPVLEALNADPNATA
                     LYLYPTKALAQDQQRKIRDLWPDLCFGTYDGDTPPTDRRKLKDRGRLLLTNPDMLSGS
                     VLPHHDSFNRFFSNLKYVVIDEVHAYKGAFGSHVSIVLARLKRVAEKYGAKPQFIMTS
                     ATIRNPKELAEGLIKEPVSVVDNDGAPKGQKTFVLWNPPFDESKGVRLSANAEANRLF
                     VSLIKKRVRTIAFVRARVTAELLYRYAVDELASYGLADKVRPYRGGYLPEDRRQIEQA
                     LFSGELLGVTSTNALELGIDIGGLDACIIVGYPGSVSSVWQQAGRAGRGRDESLVFLI
                     ANADPIDQFIVQHPDYFFAQSPERAVIDPDNPYILLGQLRAMAYELPLKKDEVAQLSE
                     NAPAVTELLAEGGQLVPRGNRFFWMAKGYPANDINLR"
BASE COUNT          579 a          577 c          588 g          497 t
ORIGIN      
        1 aacgctggaa gaatacgcag atcaagccgt agcagtgggt ctttctgaga taggcttttc
       61 cgatcatctg cccttatctc cggcgccagc ggatgtgctg agtcgggatt gggccatgaa
      121 cgaagaagag ctccctgagt atgtggaaga catctacagc ctccagcgga agtacccgag
      181 tcttcccatt aaactcggca ttgaagccga ctacttcccc gatcgcacca gcattgcaaa
      241 aactgagaag atgcttcagg cctttagctg ggactatgtg atcggttcgg tacaccattt
      301 ggggtactgg actgtggatt cgtctgagta cctccacgag tttgaaagaa gagatctgta
      361 taaggtttat gaagactact tcgaaactgt gatcaaagca gcaaactccg gacttttcga
      421 tgttctgggt catatcgatt tggttaagaa gttcggccat gtgcccagag cggatctaac
      481 gtctctttac cgggattttg cccaagagat caagaaaagc ggaatagctt ttgaaatcaa
      541 cacgtcgggt ctgagaaagc ccatcggcga gatctaccca gcagaaggcc tgctgaggga
      601 acttctgaac cttcaggttc cggtgactct tggctccgac agccacagtc ccctggaggt
      661 aggcaaagac tttgacaaag ccctagagct gctcaaaaga tacggcacag atcggatcgc
      721 tacgtttacc aaaagacagc ggatcatgag acctctagag gtataaggtg gtgcagccca
      781 tgctagatgt gcaagcgttt ttaagagaga tcaccagcat ggaagagtac aaaaaccaaa
      841 taactgctgt gaagaccata ccggcggaaa aacccgtcta cggagatgca gatctcggcg
      901 agtttttgga aagcttgctg aagcgggttg gcattgaaaa actctacagc catcaggcca
      961 cagcactgga gaaagccaga agaggagaaa accttgtggt tgtttcgggt accgctagcg
     1021 gcaagaccct ctgctacaac ctgccggttt tagaagcgct gaacgccgat cccaacgcta
     1081 ccgcgctgta cctctacccg acaaaagccc tagcccagga tcagcagagg aagatcagag
     1141 acctttggcc cgatctgtgc tttggtacct acgatggcga cactccgcct accgatcgac
     1201 gaaaactcaa agatcgggga aggttgcttt taaccaatcc ggatatgctc agcggcagtg
     1261 tcctgccgca ccacgactcc tttaatagat ttttcagcaa tttaaaatat gtggttatcg
     1321 atgaggtgca cgcgtataag ggagctttcg gctctcacgt ttccatagtc cttgccaggt
     1381 tgaagcgagt tgccgaaaaa tacggtgcaa aaccccagtt catcatgacc agcgccacta
     1441 ttagaaaccc caaagaactt gcagaaggcc tcatcaagga accggtttca gtggtagaca
     1501 acgacggggc accgaaggga cagaagacct tcgttctctg gaacccgcct ttcgatgagt
     1561 cgaaaggcgt gcgcctcagt gccaatgctg aggccaacag gctgtttgtt agcctgatca
     1621 aaaaaagggt tcgcactatt gcctttgtca gagcgagggt gacggcagag ctcctctatc
     1681 gatatgcagt agacgagctt gccagctacg gacttgcaga caaagtacgg ccataccgcg
     1741 gcgggtatct tcctgaagac cgccgccaga tcgagcaggc gctattctct ggagagcttt
     1801 tgggggtaac ctctactaac gctttagagc tcggcatcga tatcggcggt cttgatgctt
     1861 gcatcatcgt cgggtatccc ggctcggtgt cctctgtgtg gcagcaggcc ggacgagctg
     1921 gcaggggcag ggacgagtcc ttggtctttc ttatcgccaa tgccgatcct atcgaccagt
     1981 ttatcgtcca gcatccagat tacttctttg cccagagccc tgagcgagcg gttatcgatc
     2041 ccgacaatcc ctacattctc ctcggccagc tgagagcaat ggcctacgag ctacctttga
     2101 aaaaagatga agttgcccag ctcagtgaaa acgccccagc ggtaaccgaa cttcttgcag
     2161 aaggcggtca gctggttcca agaggtaacc gcttcttctg gatggcaaaa ggctatccgg
     2221 caaacgatat caacctacgc a
//
LOCUS       DAOSDA010000303         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_32193_length_2220_cov_4.145035, whole genome shotgun sequence.
ACCESSION   DAOSDA010000303 DAOSDA010000000
VERSION     DAOSDA010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32193_length_2220_cov_4.145035"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PLW82_07045"
     CDS             complement(<1..529)
                     /locus_tag="PLW82_07045"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ47067.1"
                     /translation="MKAIAITAAVLTGTLIISSLYFYNLAIARGPKEFLSRSPDLQRG
                     TSGENSVSLERTFSPTTWLDSQNPARWEIQSDDGLKLVGLFIPASTPTNRTVICAHGY
                     TSQSRHMAQFARFFHEKLGFNVLLPDARGHGESEGDYIGFGWHERKDYLLWIQRIIEE
                     VGEDSQIVLFGISMGG"
     gene            complement(668..1933)
                     /locus_tag="PLW82_07050"
     CDS             complement(668..1933)
                     /locus_tag="PLW82_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOJ47068.1"
                     /translation="MNLAIGLFVATYILLLVFPKIRAYVALTSAALFVILGILPIGKV
                     FGAVDWNVILMIAGTMGIVALFIESKMPALLADLILAKIPNVKWAIISLSLFAGIISA
                     FIDNVATVLIVAPVALTIAKKLNISPVPSIIAISISSNLQGAATLVGDTTSILLGGHA
                     GMDFLDFFVYRGMPGLFWIVQIGAIAATLVLVYVFRKYTEPIETLELTTVKDYFPSYL
                     LVGMVVLLILASFIPNKPAITNGIITMSLFVVGLIRSYIKTKDFNSILSAVNEIDYFT
                     ILLLAGLFVVIAGITEAGVIDAISKLFVKVSGGNVFVIYTLIVWASVLFSAFIDNIPY
                     VATMLPVTGYIASSLGLDPTLLYFGLLVGATLGGNLTPIGASANITALGILRNDGHEV
                     SAGEFMKVSVPFTLAAVTTGYILVWLLWA"
BASE COUNT          606 a          520 c          560 g          534 t
ORIGIN      
        1 ccccgcccat ggagatgcca aaaagtacga tctgtgagtc ttcacctact tcctcaatga
       61 tccgctggat ccagaggagg tagtcttttc tttcgtgcca accgaagccg atgtagtcgc
      121 cttcgctttc cccatgcccc ctagcgtcgg ggagcagtac gttgaaaccg agcttttcgt
      181 ggaaaaacct ggcaaactga gccatgtgcc tggactgaga cgtgtagccg tgggcgcaga
      241 tcaccgtcct gttggttgga gtgcttgcag ggatgaacaa tccgaccagc ttcaacccgt
      301 catcagactg gatctcccac ctggccgggt tctggctatc cagccaggtt gtaggggaga
      361 aggttctctc caaagaaacg gaattctcgc cgctagttcc tcgctggagg tccgggcttc
      421 ttgaaagaaa ctccttcggg cctcgtgcta tagcaagatt atagaagtat aagcttgata
      481 taataagagt cccagtgaga acagctgctg tgatagcaat ggctttcatt ctgtcgtctc
      541 tcctttctct ttctttttgc ttacaggttt aaatttccgc aaatccccgt gtcaacctcc
      601 agattgaaca taaaaaaacg caccgaaaag gtgcgttgtt cttatctccc ttgagatggg
      661 cattagttta tgcccaaagc agccacacca ggatgtagcc ggtggtaacg gctgcaaggg
      721 taaagggtac gctgactttc atgaactccc cagcgctcac ttcgtgcccg tcgtttctga
      781 ggatgccgag ggcggtgatg ttggcagaag caccaatggg ggtgaggttc cctcctaatg
      841 tcgcacctac cagaagtccg aagtagagca aagtgggatc taagcccagg cttgaggcaa
      901 tgtagccggt taccggaagc atggtcgcca cgtagggaat gttgtcgata aacgcggaaa
      961 agagcacaga agcccaaacg atgagggtgt agataacaaa gacgttgcct ccgctgacct
     1021 tcacaaacaa cttgcttata gcatcgataa cgccagcttc agtgattcct gcgatcacaa
     1081 caaaaagtcc agctagaagc agaatggtga agtaatcgat ctcatttaca gcacttagaa
     1141 tggaattgaa atctttcgtc ttgatgtagc ttctaatgag tcccacgaca aatagcgaca
     1201 tggtgatgat gccgttggtg atggccggtt tgttaggaat gaaggaggcc aggattaaaa
     1261 gcacaaccat gcctacgagg agataactcg ggaagtagtc tttaacggtt gtcagttcca
     1321 gagtctcgat aggttcggta tatttcctga agacgtagac aagtactagc gtagctgcga
     1381 tagccccgat ctgaactatc cagaaaagac caggcattcc ccgatatacg aaaaagtcca
     1441 agaagtccat gcctgcatgt ccaccaagga ggattgaagt ggtgtcacct accagagtgg
     1501 ctgcaccttg gaggtttgag gagatggaga tagcgatgat gcttggaaca ggtgagatgt
     1561 tcagcttctt ggcgatggta agcgctacag gcgcaactat caggacggtt gcaacgttgt
     1621 caatgaaagc ggaaatgatt ccggcaaata gcgaaagcga gataatcgcc catttcacgt
     1681 tggggatttt cgccagtata aggtctgcaa ggagagcagg catcttggac tcgataaaaa
     1741 gcgctacgat tcccattgta cctgcgatca tcagaattac gttccagtcc actgcgccga
     1801 acactttacc tatcgggagg atccctaata taacgaaaag ggctgccgat gtaagggcaa
     1861 cgtacgctct gattttagga aaaaccaaca aaaggatgta ggttgcgaca aaaagtccta
     1921 tcgctaagtt catgaaagat gctcctttca gttctataag gctcagagat taacctaata
     1981 ggttttctgg aacggagcag taaatcctgc atcaagagaa aagtagctcg gtgttctgca
     2041 gataaaagcc cgatgttcta ctaccaatag actattcaat gcctctgcaa aatgcagtcg
     2101 aaatgagcaa aaaagcaggc agagatttcc agagatttct gcctgctggg ttagatctat
     2161 ggttcaacta tcaccagtcc cgcttcaggt tcaactctgc catcaaagat cttttcgtac
//
LOCUS       DAOSDA010000304         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_32283_length_2216_cov_2.847756, whole genome shotgun sequence.
ACCESSION   DAOSDA010000304 DAOSDA010000000
VERSION     DAOSDA010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32283_length_2216_cov_2.847756"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1356)
                     /gene="infB"
                     /locus_tag="PLW82_07055"
     CDS             complement(10..1356)
                     /gene="infB"
                     /locus_tag="PLW82_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOJ47069.1"
                     /translation="MSKIRLYEMAKKLNVTSKELINYLKDFNVEVPSHMSTLDDEVVD
                     LLNEAFSDEDEIVEEDFEEIEKAKTKKKPAKKKTKKKVITIEEQLTVARLAEKLAIPT
                     TELIKKLMDLGMMLNQNAPVDFDTASLLAEEYGAKLEMKAAEQDESESVEIEEEPENL
                     EPVPRAPVVTIMGHVDHGKTSLLDKIRQSHVAQSEAGGITQHIGAYQVELEGGKITFL
                     DTPGHEAFTSMRARGAQVTDIAILVVAANDGVMPQTLEAISHAKAANVPIIVAINKID
                     APGANPDRVKQQLSEHGLIPEDWGGDTICVPVSALRGDGIGELLEMINLVAEMQELKA
                     PVDGLAKGTVIEAKLDKFRGPVATVLIQKGTLKVGDPVVAGTVCGRVRALTNDKGQNI
                     KTAGPSTPVEVLGLGDVPHAGDLFQVTTSEKQAREIAAKRLSKEKEEMQKEENKQS"
     gene            complement(1357..1653)
                     /locus_tag="PLW82_07060"
     CDS             complement(1357..1653)
                     /locus_tag="PLW82_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOJ47070.1"
                     /translation="MSMLGIARKSGALLIGSKAAEEAIVSNRARLIILCADAGNSTKR
                     NFRHLAEKCQVELVLFPSKIQLGKLIGVKEAAVVVLTEDSFAGRVKELINSGGE"
     gene            complement(1650..1913)
                     /locus_tag="PLW82_07065"
     CDS             complement(1650..1913)
                     /locus_tag="PLW82_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOJ47071.1"
                     /translation="MVKKIPERSCIGCRGKFPKRELIRIVRTPESEVKIDPTGKQNGR
                     GAYICPKLECLELAFKGDRLGRALNAEVSKEIFERLKEELANR"
     gene            complement(1894..>2216)
                     /gene="nusA"
                     /locus_tag="PLW82_07070"
     CDS             complement(1894..>2216)
                     /gene="nusA"
                     /locus_tag="PLW82_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283341.1"
                     /note="modifies transcription through interactions with
                     RNA polymerase affecting elongation, readthrough,
                     termination, and antitermination; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HOJ47072.1"
                     /translation="AVVEELHGERIDIIPWDPDPSVFVANALSPARVNSVEILEGEEK
                     IARVIVDDDQLSLAIGREGQNARLAAKLTGLKVDIKSVSQIDQDQVESQEEVGDAEDG
                     QEDS"
BASE COUNT          441 a          602 c          489 g          684 t
ORIGIN      
        1 ctacaaatat catgattgtt tattttcttc tttttgcatc tcttcttttt ctttgctgag
       61 acgctttgcg gcaatttctc ttgcctgctt ttcgctggtg gtaacttgga acaagtctcc
      121 tgcgtgagga acatcgccta ggccgagaac ttcaacaggt gtcgatggtc cggcagtctt
      181 gatgttttgg cctttgtcgt tggtcagggc tctaaccctg ccgcaaactg taccggccac
      241 caccggatcg cctactttaa gggtaccttt ctggatcaaa acggtggcta ctggaccacg
      301 gaacttgtcg agtttggctt cgataacggt gccctttgcc agtccgtcta caggtgcttt
      361 aagctcctgc atctctgcta ctaggttgat catttccaga agttccccta tgccgtcgcc
      421 tctaagggcg gaaacgggga cacagatagt gtctccaccc caatcctcag gtatgagacc
      481 gtgttctgac agctgctgct tgacgcggtc cggattggca cctggagcat cgattttatt
      541 gatagccaca atgataggca cgttagcggc cttggcgtgg ctgatagctt cgagagtctg
      601 cggcattacg ccgtcgtttg cagccacgac taagatcgca atatcggtta cctgtgcgcc
      661 tctagccctc atggaagtga aggcctcgtg cccaggtgta tccaggaagg taattttgcc
      721 accttctagt tcaacctggt aggcgccgat atgctgcgtg attccgcctg cctcgctttg
      781 agctacatgg ctctggcgga ttttgtccaa aagtgaagtt tttccgtggt cgacgtggcc
      841 catgattgta acaaccgggg cacgtggcac aggttcgaga ttctctggtt cttcttcgat
      901 ctctacactt tcactttcgt cctgttctgc ggctttcatc tccaacttcg caccatactc
      961 ctcagccaaa aggctggcgg tatcaaaatc caccggtgcg ttttgattca gcatcatacc
     1021 cagatccatt aacttcttaa tcagttcggt tgtgggaatc gccagtttct ctgcgagtct
     1081 ggctacggta agttgttctt ctatagtaat aaccttcttc tttgtctttt tcttggccgg
     1141 ctttttcttg gttttggctt tctctatttc ctcaaagtcc tcttcgacaa tttcatcttc
     1201 atccgaaaac gcttcgttga gtagatccac cacttcgtcg tctagtgtac tcatgtggct
     1261 gggtacttca acgttgaagt ccttcaagta gtttatgagc tccttagaag tcacattgag
     1321 tttcttcgcc atttcgtaaa gacggatttt gctcattcac tcacccccgg agtttatcag
     1381 ttctttcact ctgcccgcaa agctatcttc ggtcagaacg accaccgctg cctcttttac
     1441 gccaatgagc tttcccaact gaatcttact ggggaaaagc accaattcca cttgacattt
     1501 ctcggccaag tgtctgaaat tgcgtttagt ggaattgcct gcatcagcgc acagaatgat
     1561 aaggcgtgct ctgttactta cgatggcctc ttcggcagct ttgcttccaa tcagcaaagc
     1621 tccggatttt cttgcaatcc ccaacatgct catctatttg ccagttcctc tttcaatctc
     1681 tcgaagatct cttttgacac ttcagcattc agcgcacggc cgagcctgtc gcctttgaag
     1741 gccaactcca aacattcaag cttaggacag atgtaagcac ctctgccgtt ttgcttgcca
     1801 gtaggatcga tctttacttc cgactctggc gtccgtacga ttctgatgag ttctcttttg
     1861 gggaacttac ctctacagcc tatgcagctt cgttcaggaa tcttcttgac catcttcagc
     1921 gtcacccacc tcttcctggg attcgacctg gtcctggtca atttgactta cgcttttaat
     1981 gtcgactttc agtccggtga gttttgctgc aagccgtgca ttttgccctt ctctaccgat
     2041 agcaagagac agctgatcgt cgtctacgat cactctggca atcttttcct cgccttcgag
     2101 gatctctaca gaattaactc ttgctggaga cagtgcattg gctacgaaaa cactaggatc
     2161 gggatcccat ggaatgatgt cgattctttc gccgtggagt tcttccacca ccgccc
//
LOCUS       DAOSDA010000305         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_32957_length_2183_cov_3.913534, whole genome shotgun sequence.
ACCESSION   DAOSDA010000305 DAOSDA010000000
VERSION     DAOSDA010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32957_length_2183_cov_3.913534"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /gene="miaB"
                     /locus_tag="PLW82_07075"
     CDS             complement(<1..1194)
                     /gene="miaB"
                     /locus_tag="PLW82_07075"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOJ47073.1"
                     /translation="MKRLSKTVKLFAILTFGCQMNVRDSETIKGILESLGYIETDNPD
                     EADLLLFNTCSVRENPERKVYGRIQQYKEDSGKIIGICGCMVQQGAEFEYIKNELPQV
                     RLVFGTHNIHELPELLEKAEEGQRVLNVWEDKQEIVEGLPAKRDQGVKAFVNISYGCN
                     NFCTYCIVPYTRGREKSRTPENIISEVRELAQNGYKEVTLLGQNVNSYGKDLGLDFAA
                     LLEKVHEVEGIERIRFTTSHPKDLSDRLIAAFKNLPKLCEHLHLPVQAGSDRILKKMN
                     RRYTREQYLELVAKLRDAVPGIALTTDIIVGFPGETEEDFLQTLDIVEKVRYDSAFTF
                     IYSPRKGTPAAEMPDQVDEEVKKERIYRLIDLQNRISGEINQEIVGSTVEVLVEGTSK
                     TDPSRL"
     gene            1514..1783
                     /locus_tag="PLW82_07080"
     CDS             1514..1783
                     /locus_tag="PLW82_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOJ47074.1"
                     /translation="MQTYCLDRIPLNQSGIILYIDKTSKIRRRLLDLGFTKGTEVAAV
                     FSSPSGNPKAYYIRGTVIALRNEDAAEVKIQLLGSGEGKENGTYA"
     gene            1767..>2183
                     /locus_tag="PLW82_07085"
     CDS             1767..>2183
                     /locus_tag="PLW82_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOJ47075.1"
                     /translation="MGLTLSSTGLELLKSQDQNPKTSEGTVKVALLGNPNTGKSTVFN
                     ALTGLKQHTGNWPGKTVEVATGRFSFDGKEFLLVDLPGTYSIFADSPEEEIARDFLLF
                     SKPDVTVVVVDAACLERNLQLLLQVIEISPRTVLCVN"
BASE COUNT          505 a          607 c          514 g          557 t
ORIGIN      
        1 aagcctcgat gggtcggttt tgctagtccc ttcaaccagc acttccacgg tacttcctac
       61 aatctcctga ttgatctcgc cggagattcg gttctgcagg tcgatcaacc ggtagatgcg
      121 ctctttttta acttcctcat cgacctgatc gggcatctcg gccgcaggcg tgcctttcct
      181 cggcgaatag atgaacgtga acgccgaatc gtagcggacc ttctctacga tatccaatgt
      241 ctgcaggaag tcttcttctg tctctccggg aaagcccaca atgatgtctg ttgttagggc
      301 gatgccggga actgcgtctc tgagttttgc caccagctct aggtactgct ctcgggtgta
      361 ccgcctgttc atcttcttca agatgcgatc gcttccggcc tgaacaggca gatgcagatg
      421 ttcgcagagc tttggcagat ttttgaacgc agcgatcagc ctgtctgaaa gatccttggg
      481 gtgggacgta gtaaagcgga tgcgctctat tccctccact tcgtgaacct tctccaaaag
      541 cgctgcaaaa tccagcccca ggtctttgcc gtaggagttg acgttttggc caagaagggt
      601 tacctccttg tacccgtttt gtgccagctc cctgacctca ctgataatgt tttcgggagt
      661 gcgcgacttc tccctgcccc tcgtgtaggg tacgatacaa taggtgcaga agttgttgca
      721 gccgtaagag atgttgacaa aggccttcac accttgatct ctctttgctg gaagcccttc
      781 tacgatctcc tgtttatcct cccagacgtt gaggactcgc tgcccctctt cggccttctc
      841 caaaagctcc ggcagctcgt gaatgttgtg ggtgccgaac accaggcgga cttgagggag
      901 ctcgttcttg atgtactcaa actcagcacc ctgctgcacc atacagccgc agatcccgat
      961 aatcttgcca gagtcctcct tgtactgctg aatcctgccg tacaccttcc gttcaggatt
     1021 ctccctaacc gagcaggtgt tgaacaaaag caggtcggcc tcatcggggt tgtctgtttc
     1081 aatatatcct aagctttcta aaatcccttt gattgtctct gaatctcgca cattcatctg
     1141 gcagccaaag gttagaatag caaacaattt cacggtctta gacaaacgct tcatctcctt
     1201 attgcagcta aatacctcta tcttagaata gcttatttcc gaggtcaaaa caacctccca
     1261 aaaccgctcc caagagagcc tctgcgcagg ctgcagtaga aagcggcact gttatgccat
     1321 accccaagag tccggcacaa ccttggggtc ttttacatca tattcccaga tgtctcttgt
     1381 agaaaacttc agcatagata gcattcccaa caagcgaacc gctttctgct gactgcctca
     1441 ccgcagagca cccctctgca tatcttagga ttaaagattt taaccgcagt taactttctc
     1501 tggagggaat gagatgcaaa catattgtct ggatcggatt cccctgaatc agtccggcat
     1561 tattctctat atagacaaaa ccagcaaaat ccgccgccgc cttttggacc ttggctttac
     1621 caagggcaca gaggttgccg cggttttcag ctctccttcg ggaaacccca aagcttatta
     1681 tattagagga acggtgatcg ctctgaggaa cgaagacgca gcggaagtca aaatccagct
     1741 cttgggctct ggtgagggta aggaaaatgg gacttacgct tagctccaca ggtctagagc
     1801 tcctcaagtc gcaggatcaa aatcctaaga ccagcgaagg caccgtcaaa gtggcgcttc
     1861 tcggcaatcc caacaccgga aagagcacag tgttcaacgc cctgacggga ttaaagcagc
     1921 acaccggaaa ctggccggga aagaccgtgg aggttgcaac aggcaggttc tcctttgacg
     1981 gaaaggagtt tcttttagtt gaccttccgg gcacatactc cattttcgcc gactcccccg
     2041 aggaggagat tgctcgggac ttccttcttt tctccaaacc cgacgttaca gtggtagtag
     2101 tggacgcggc ctgcctggaa aggaacctcc agcttctcct tcaggtaata gagatctccc
     2161 cacggacggt gctatgcgtc aat
//
LOCUS       DAOSDA010000306         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_33825_length_2146_cov_3.339550, whole genome shotgun sequence.
ACCESSION   DAOSDA010000306 DAOSDA010000000
VERSION     DAOSDA010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33825_length_2146_cov_3.339550"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1872)
                     /locus_tag="PLW82_07090"
     CDS             complement(<1..1872)
                     /locus_tag="PLW82_07090"
                     /inference="COORDINATES: protein motif:HMM:NF041464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ47076.1"
                     /translation="MGSAKELEIKHLKKIVGIIQDELVDLENRIAKSKERVLTVRKYL
                     WEESPRLVRDATDANELAQGLLELQRVEESLAFYLKEKNRLERLKNSPYFARIDVKFN
                     GSAETIYIGVNGFIHPKTYQNLIYDWRTPIASLFYDFPLGEASYVCDLGVVHGEITLK
                     RQFRIEKSQMLYMFDSSINIEDEMLQEILSQSTDGRMRDIVMTIQKEQNQAIRDEKHD
                     LVVVWGPGGSGKTSIALHRIAYLLYKKRESLSANNIVIFSPSSIFQDYISEVLPNLGE
                     ENVKQTTFYRLAKRIVNPEGYSLEHDFEEYESLFQSDIESVKKRTIVYKNSPQFLEVL
                     ENYARYLEERYRNVPDFVYNGETVLTGEEFYSCVKEKYAYLPFKKRLEKLERRLHALL
                     EPLEKRREEEIFKGLLRANLQKKSEAGLRREAKRRALASSVELKERIAKLVSLDLFEE
                     YAKLFQNPLLFQKLAGAGSIESLSEIMAYTARCLAEKRLLKEDLAALAFLKQRLEGSF
                     LADNIKHVVIDEVQDYSALELKVIAQAFSEASFTVLGDTNQSLLERGNPEEYVAESIG
                     IEKANTINLQKTYRSTVEITSFSAALLGLEKKIEPVLRKGKKPLVILKQDPRRLAALL
                     "
BASE COUNT          493 a          565 c          490 g          598 t
ORIGIN      
        1 caggagcgct gccaaccttc ggggatcctg ctttaggatc actaaaggtt ttttgccttt
       61 tctcagcacc ggctcgatct tcttttccag ccccaatagg gctgcggaga aactggtgat
      121 ctccacagtg gacctgtaag tcttctgcag gttgatagtg ttggcctttt cgatgccgat
      181 gctctctgcc acgtactctt cgggattgcc cctctctaaa agagactgat tggtatcgcc
      241 gagcacggtg aaagaagctt cggagaaagc ctgagcaatg accttcagct ctaacgccga
      301 ataatcctga acctcatcga tcaccacatg tttaatgtta tctgcaagaa aacttccctc
      361 taaccgctgt ttgagaaagg ccagagctgc aagatcctct ttaagcaggc gcttttctgc
      421 aaggcagcgg gcagtgtaag ccataatctc gcttaagctc tctatggaac ctgctcctgc
      481 aagtttttgg aagaggagcg gattttggaa gagctttgca tactcttcaa acagatccaa
      541 gcttacaagc tttgcgatgc gctccttcag ttctacgctg ctagccaaag ctctacgctt
      601 tgcttccctg cgcagcccag cttcggactt tttctgcagg ttagctctga gaagcccttt
      661 aaagatctct tcctcacgtc gtttttccag cggctccaga agcgcgtgca gtcttctttc
      721 cagcttttcc aggcgctttt taaacggcag gtaggcgtac ttctccttca cgcagctgta
      781 gaactcctca cctgtcagta ccgtctcgcc gttgtacaca aagtcgggaa cgtttctata
      841 ccgttcttcg aggtatcttg catagttttc caaaacctcg agaaactgcg gcgagttttt
      901 gtagacgatg gtgcgttttt tcaccgactc tatatcgctt tggaataagc tctcgtactc
      961 ctcgaagtca tgctccaggc tatagccttc gggattaaca atccgttttg ccagtctgta
     1021 gaaggtagtc tgcttgacgt tttcttcgcc tagattggga agaacctccg aaatgtagtc
     1081 ctggaagatg ctgctgggag aaaaaatgac gatgttgttt gcactgaggc tttccctctt
     1141 cttgtagaga aggtaggcga tgcggtgcag ggctatagaa gtcttgccgc ttccacctgg
     1201 cccccagacc acaaccaaat cgtgcttttc atcgcgaatt gcctgattct gctccttctg
     1261 aatggtcatg acgatgtctc tcatgcgtcc gtctgtggac tggctgagaa tctcctgcag
     1321 catctcgtct tcgatgttga tactgctgtc gaacatgtag agcatctggc ttttttctat
     1381 gcggaactgc cttttcaggg tgatctcgcc gtgaaccaca ccgagatcgc aaacatagct
     1441 agcttcacct aaaggaaagt cgtagaagag acttgcaata ggcgtgcgcc agtcatagat
     1501 caggttctgg taagtcttgg gatgaatgaa gccgttgacg ccgatataga tggtctcggc
     1561 gctgccgtta aacttcacat cgatgcgggc aaaataagga gagtttttga gcctttccag
     1621 ccgattcttc tctttgaggt agaacgccaa actctcttcc actctctgca gttccaagag
     1681 cccttgtgcc agttcattgg catctgtagc gtcgcgcaca aggcgtggag actcctccca
     1741 gaggtacttc ctgactgtca gcacacgctc cttgcttttt gcaatgcggt tttccagatc
     1801 aaccagttcg tcttgaataa tacctacaat cttcttcagg tgtttgatct ctagctcctt
     1861 ggcactaccc acaactatcc ctcctcaggc tgctgcatct cccatatcct atatattata
     1921 tcaaaatctc agtttactgt gcccgctttt gccaattctg cgataacggt acttgacgta
     1981 aaaaaaccta gtctgggata gcagcaagca gagagctcaa aataaagcag cccggaacga
     2041 gaaagaccaa tgcaccagct acaactcccg aatcgttgaa agccaccgca aaagatgcag
     2101 tgatcactgt gagaagaagc agtttctgca gtgcttcccc ttcttg
//
LOCUS       DAOSDA010000307         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_34280_length_2128_cov_3.503618, whole genome shotgun sequence.
ACCESSION   DAOSDA010000307 DAOSDA010000000
VERSION     DAOSDA010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34280_length_2128_cov_3.503618"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLW82_07095"
     CDS             complement(<1..613)
                     /locus_tag="PLW82_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ47077.1"
                     /translation="MIPRPEHPRPDLLRKDWQNLNGIWDFRFDFGESGYEEEAHLGKG
                     FDKKINVPFAPESTLSGIGYTGFMNSVWYKRTVTVPEQFRDYRVILHFGAVDYLASVY
                     VNGMLAGEHRGGYTPFKIDITDLLQDGENLLVVCAKDNNRLGLQPRGKQASKLYSSGC
                     DYTRTTGIWQTVYLEYVKGGFIDDLHIIPDALNCSVKVSGVINK"
     gene            820..2115
                     /locus_tag="PLW82_07100"
     CDS             820..2115
                     /locus_tag="PLW82_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ47078.1"
                     /translation="MSKKQLLVLIVVVLITSSLAGAVTLEIWQASSNEQLAIMKSFIE
                     ERFTPKTGISVNLSVIPGGDDQWNKVLLAMASNDTPDLAVIGSEWPLEFGIRGGLVDM
                     REMFGEEYDKLFATAFPGLNESLTYYNTGFGLNSSFGQTLTYYRTDIFAENGWEVPAT
                     WDEARALLPKMQARDMNIGSGSWYLTPDWFGAYIFMWQHGIREVNEERTRTTWDTPEA
                     IAAFTEFMELFTVHGIPLESVPFIEAISRGDYPFIVGISWGYSDITLGAPQLKGKWDI
                     CLIPGKKQPDGTINHAAYIGGNPYVMFKNSKHKEEAFEFLKWWLSAEVQREFADMVWN
                     KLQIIYQPANMEAYNSVTFLPESHRRILNIQAEQSRAPLFALGLVVSRRNLTNAARAV
                     VLTGADPKAELLKAAEISNDELARKQKEYARFIKGLIQE"
BASE COUNT          511 a          542 c          546 g          529 t
ORIGIN      
        1 ttttattgat cactccggaa acctttacgc tacagttcag agcatcgggg atgatgtgga
       61 gatcatcgat gaaaccgcct ttaacgtact ccaagtatac agtctgccag atgccggtgg
      121 tacgggtgta gtcgcaaccc gatgaataga gctttgatgc ctgcttccct ctgggctgta
      181 ggcccagacg gttgttgtcc ttagcgcaga caacaagcag gttctctcca tcctgcaaaa
      241 gatcggtaat gtcaatttta aacggcgtat agcctcctct gtgttcgccg gcaagcattc
      301 cgtttacgta gacggaggcc aagtagtcca cagcaccgaa gtgcagaata accctgtagt
      361 ctctaaactg ctcgggcacg gtaacggttc gcttgtacca gacgctgttc ataaatccgg
      421 tatatccgat accggacaga gtgctctccg gtgcaaacgg aacgttgatc ttcttatcga
      481 atcccttgcc caaatgggct tcttcctcgt agccgctttc gccgaagtca aaccgaaaat
      541 cccaaatgcc gttaaggttc tgccagtctt tgcgcagcaa atctggcctt ggatgttctg
      601 gtcttggtat cacctttgtc tctcccccag ttcttgctgt tttttgcagc cgttttctgc
      661 cgagttgcct gaaaaggcac ataactttac tgcaaaagtt cccttttttc tagagttacc
      721 ctgctgcaaa tcgcttcttt tttctgcgtt tttcctgctt ttggtactgg cagttgtgct
      781 atactgattt tgcagatcaa tgcttgggag gaagagagta tgtcaaaaaa gcaattattg
      841 gttctcatcg tggtagtgct tataacttca agcctagccg gtgctgtcac cctggagatc
      901 tggcaggcat ctagcaacga acagctggcg attatgaaga gctttattga agagcggttt
      961 acgccaaaga ccgggatctc ggtgaacctc agcgtcatcc ccggaggcga tgaccagtgg
     1021 aacaaggtgc tcttagctat ggccagcaac gataccccgg atcttgctgt cattggctcg
     1081 gagtggcctc ttgagttcgg cattcgcggc ggactggtag acatgcggga gatgttcgga
     1141 gaggagtacg acaagctctt tgccacggcc tttcccggtc tcaacgagtc cctgacctat
     1201 tacaataccg gctttggact gaactctagt tttggccaaa ccctaaccta ctaccgcact
     1261 gatatctttg cagaaaacgg ctgggaggta cctgcaactt gggacgaggc cagggcgctt
     1321 ctgccgaaga tgcaggctag ggacatgaac atcggttcgg gttcctggta cctgactccc
     1381 gattggtttg gtgcatacat cttcatgtgg cagcacggca tccgtgaagt gaacgaagag
     1441 cgcaccagaa cgacttggga cacacctgaa gccatcgccg catttacaga gtttatggag
     1501 ttgtttaccg ttcacggaat acctcttgag agcgtgccgt tcatcgaagc cataagcaga
     1561 ggggattacc ccttcatagt gggcatcagc tggggctatt cggatattac cttaggcgca
     1621 ccgcagctga aaggcaaatg ggatatctgc ctgattcccg gaaagaagca gccagacggc
     1681 actatcaacc acgcggccta catcggtgga aatccgtacg taatgttcaa gaactcaaag
     1741 cacaaagagg aagctttcga gttccttaag tggtggctca gtgcagaggt ccaaagggag
     1801 tttgccgata tggtctggaa caagctgcag atcatctacc agcccgccaa catggaagct
     1861 tacaactccg ttactttcct gccggaatct caccgcagaa ttctcaacat ccaagcagag
     1921 caatccagag ctccgctgtt tgctttggga ctggttgtca gcaggaggaa cctcaccaac
     1981 gctgcacggg ccgttgtcct aaccggtgca gatccgaaag cggaacttct caaggccgct
     2041 gagatctcca atgacgagct ggcaagaaag cagaaagagt atgcccggtt tatcaagggt
     2101 ctaatccagg agtaaatgga gaacggta
//
LOCUS       DAOSDA010000308         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_34305_length_2127_cov_3.175193, whole genome shotgun sequence.
ACCESSION   DAOSDA010000308 DAOSDA010000000
VERSION     DAOSDA010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34305_length_2127_cov_3.175193"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PLW82_07105"
     CDS             complement(<1..527)
                     /locus_tag="PLW82_07105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ47079.1"
                     /translation="MEGPRAARKEELQDLIDLINYVFRVSASKEPDMHICFPLFISEN
                     NLDNLLVYADRKTGKPISHLGLWINDFSYFGESLKIGYIGSVCTHPDYRGQGLASKLL
                     DVAFQQLEQNGVDLLLVSGSRSLYVRAGCRVVGVVNWYTIPKKEQDVWFDVTVDPPVE
                     PLNELWMKEPLRTKRG"
     gene            complement(621..>2127)
                     /locus_tag="PLW82_07110"
     CDS             complement(621..>2127)
                     /locus_tag="PLW82_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOJ47080.1"
                     /translation="PIKPNPNVFLQVSLLGLLVTVLFSVLPVLRFTKIRPLAIYRDDN
                     PERLLSPESKLTSFLVIILLAGIFGLFIGLILDSLLAGIIFTFATLILTAIALGIIKL
                     IIKLVLYLPIYFSTPAKMARRSLFRQSGRTAAAVLALSFGISAIVLISFIQTDVLSFM
                     HGLRDDQNTPNIFALLDQQNGPPVESIVEFMEQDSRIESFSTMKLLTSSLEIVNDRLY
                     DPTTASDSRSAILAMAFLIAAADPENMPTELNYVEGEPLNSDGQIVIEEHVAKSLGLK
                     IGDRLGLRVGTDRIYLNLVGFYSNMQTGVNVTSSSYATKASLEGLSPEFSTEFLFART
                     KAGVSGEEVISSLRAAYPNMGFAFDVGQLINLFDTVFSAISRFMQFLGFFALASGLII
                     LAGTMVLTKWEKRRETALIRCLGGTTKDVISVQLWENGVLSILSAGIGITFANILSMV
                     INRYVLDIDFVFNKLTNTTAFVLVLLAVTAVGAVSLWDVLHERPLSVLRNE"
BASE COUNT          564 a          504 c          560 g          499 t
ORIGIN      
        1 ccgcgctttg tgcgcagagg ttctttcatc cagagctcgt tcagtggttc tacaggagga
       61 tcgacagtca cgtcaaacca aacgtcctgt tctttcttgg gaatggtgta ccaattcaca
      121 acgcccacta ctctgcatcc tgctctcacg taaaggcttc tagaacccga cactaagaga
      181 aggtcaacgc cgttttgttc cagctgttgg aaagccacat ctagaagctt actagccaac
      241 ccctgacctc tgtagtctgg gtgggtacag accgaaccta tgtagccgat tttcagggac
      301 tcgccaaagt agctgaagtc gttgatccaa agtcccaggt ggctgatggg cttacctgtc
      361 ttgcgatcgg catacaccag gagattgtcc aggttattct cgctgataaa cagcggaaaa
      421 cagatgtgca tatctggctc tttgcttgcg ctgaccctaa acacgtaatt aattagatcg
      481 atcaaatcct gaagttcttc ttttcttgca gcacgcggtc cttccatatc tacacccctt
      541 acatgataag tttatagaat taacaagtca cgccaaaaaa agacagagta gccacggatg
      601 agttgcatcc gtggctttga ttattcgttg cgtaaaacgc taagcggtct ctcgtgtaga
      661 acatcccaca gagaaacggc gccaactgca gtaacggcca ggaggactag gacaaaagcc
      721 gttgtgtttg tcagtttgtt aaagacgaag tcaatatcta ggacgtagcg gttgatcacc
      781 atactgagaa tattggcaaa ggtaatgcca attcctgcag agagaatgct caatacgccg
      841 ttttcccaaa gctgcacgct gatcacgtct tttgtggtgc cgccgaggca gcggatgagc
      901 gcggtttccc ggcgtttttc ccatttggtc agcaccatgg tgcctgctag aatgatcaat
      961 ccagaggcta gtgcaaagaa accgaggaac tgcataaagc ggctgatggc cgagaagaca
     1021 gtgtcgaaga ggttgatcag ctgacctaca tcaaaggcaa agcccatgtt ggggtaggct
     1081 gctctcaaac tggagatgac ctcttcaccg gagactccag ccttggtcct ggcaaagagg
     1141 aactcggtgg agaattcagg tgaaagaccc tctagagacg cttttgtagc gtagctcgaa
     1201 gatgtgacgt tgacacctgt ctgcatgttg gaatagaaac ctacaagatt cagatagatg
     1261 cgatcggttc ccacccttag tcccagtctg tcgccgattt tgaggcccag ggacttggcg
     1321 acatgctctt ctatgacgat ctggccatcg gagttcagag gctcgccttc aacatagttc
     1381 aactcggtgg gcatgttttc tgggtctgct gcagctatga gaaacgccat ggcaaggatg
     1441 gcagacctag aatctgaagc tgtagttgga tcgtagagcc tgtcgtttac tatctccagg
     1501 ctggaggtaa ggagcttcat ggtggaaaag ctctctatcc ggctatcctg ttccatgaac
     1561 tcaacgatac tttcaacagg aggaccgttt tgctggtcca aaagggcgaa gatattcggt
     1621 gtgttctggt catcgcggag gccgtgcata aacgacagga cgtctgtctg aatgaacgaa
     1681 atcagcacaa tggcactgat gccaaacgac agggcaagca ctgcagcggc agtccttccc
     1741 gactggcgga acaggctacg ccgcgccatc ttggctggcg tgctgaagta gattggcaga
     1801 taaagtacca gttttataat gagcttaata atccctaagg cgattgccgt gaggatcaaa
     1861 gtggcgaacg taaagatgat cccggccaga agcgagtcta aaatcaagcc gataaacaga
     1921 ccgaagatgc ccgcaagaag gatgataacc agaaaactgg tcagtttgga ttccggcgaa
     1981 agcagtcttt cggggttgtc atcgcgatag atggcaaggg gcctgatctt tgtaaagcga
     2041 aggactggaa gaaccgaaaa gagcactgtg acaaggagtc ccaaaaggga aacctgcaag
     2101 aagacgttgg ggttcggctt aatcggt
//
LOCUS       DAOSDA010000309         2126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_34331_length_2126_cov_3.099952, whole genome shotgun sequence.
ACCESSION   DAOSDA010000309 DAOSDA010000000
VERSION     DAOSDA010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34331_length_2126_cov_3.099952"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..562
                     /locus_tag="PLW82_07115"
     CDS             68..562
                     /locus_tag="PLW82_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOJ47081.1"
                     /translation="MKEDKILEYLMAAFAGESQAHQKYLAYAKKAEREGKLNAAKLFR
                     AACDAETLHAARQFEVAGKINSTEDNLKDAIKGETYEFEEMYPEFAKAAEAVGNKAAL
                     MAFTFAMKAEEVHARLYQEALENLDETEEVFYYLCPVCGNIEKAVPEKCPICGVPGER
                     FIKY"
     gene            complement(689..784)
                     /locus_tag="PLW82_07120"
                     /pseudo
     CDS             complement(689..784)
                     /locus_tag="PLW82_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007555867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            866..1246
                     /locus_tag="PLW82_07125"
     CDS             866..1246
                     /locus_tag="PLW82_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47082.1"
                     /translation="MKFTHPENCGNAPKKLLLIQLYKAIAFADAQFLLESLEEAVVLE
                     IVGDRTVRGKDDVISEIKGFADRDLTGVHIGDVLTHGNVAAAYGTLSFRDFSVEFCDV
                     YKFGRFGPKAKITEIRSYRIPAKL"
     gene            1587..2090
                     /locus_tag="PLW82_07130"
     CDS             1587..2090
                     /locus_tag="PLW82_07130"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOJ47083.1"
                     /translation="MSRMPVVFVGHGSPMNAIEDNAYSRGWREMAKRIPKPKAIVSVS
                     AHWYTRGTRIMDEENPEAIYDMYGFPKELYEVTYNAPGSPEIAKAVKELISREIQFDN
                     SWGIDHGTWSVLVHMYLQVQLQRQKHHLGLIVIRDHLAPQLMFLTHHLMSKLLGALMP
                     PEAGMRM"
BASE COUNT          620 a          445 c          549 g          512 t
ORIGIN      
        1 agacctgcca aaagataaag tcgaaggact gcttaacttg ttaagccgaa gcgaaggagg
       61 agacactgtg aaagaagaca aaatcctgga gtatttgatg gcggcctttg ctggtgaatc
      121 tcaagctcat caaaaatatc tggcgtacgc caaaaaagcc gagagagaag gaaaactcaa
      181 cgctgcaaaa ttgtttagag ctgcatgtga tgccgagact ctgcatgccg ccagacagtt
      241 tgaggtagcg ggaaaaatca attcaaccga agacaacttg aaagatgcca taaagggcga
      301 aacctatgag tttgaagaga tgtatcctga atttgccaaa gcagcagaag ctgtaggaaa
      361 taaagcggcg ctcatggctt tcacctttgc catgaaggca gaggaagtcc acgctcgctt
      421 gtaccaagag gcgctggaaa acctcgatga aacggaagaa gtgttctact acctctgtcc
      481 ggtgtgcggg aacatcgaaa aagccgttcc cgagaagtgt cccatttgcg gcgttcccgg
      541 agaaaggttc attaagtact aaactgttac tacaaaacgc cccatctctt tcggtagtgc
      601 aacgagagat agtctagcga ttttgccaca tttaaccaac aggaactaaa aagctgccct
      661 gtccttaggg cagcttttca acatatgctc aggcgatggt ccaacctcca cctacaggaa
      721 ggacaactcc tgtcagatta gaggacatat ccgaagccag gaacagtgca gcgttggcaa
      781 catccagttc ggtttgccca tctggttttg ggtattttgt tttaagagac ctttacgtac
      841 atttacgaaa aggagggggc atcctgtgaa gtttacacat cccgagaact gtggtaatgc
      901 accgaagaag ctacttttaa tccagctgta taaagcgata gcatttgctg acgctcagtt
      961 tctgttggag agtttagaag aagctgttgt gctggaaatc gttggagata gaacggtcag
     1021 gggtaaggat gatgtaatct ctgagatcaa gggttttgca gatagggatc tgaccggagt
     1081 ccatatcgga gatgttttaa cccacggaaa tgtagcggca gcatatggaa cacttagttt
     1141 ccgcgacttc tccgtggagt tctgcgacgt ctacaagttt ggcaggtttg gaccgaaagc
     1201 aaagataacg gaaattcggt cgtatcgcat tccagcaaaa ttgtgacctt ttgtgtatgc
     1261 accgatggcc ataaaggatc tggaaagaga gacgagaaac tcatgctaaa cgggttttgc
     1321 caactatact ttggtgaggg atgcttatgg tgtttgatag gctggaggga attgaagacg
     1381 aaactgcact ggtcaacgag ttgtacaagt ttttcaatga gagtattcgc ctctcacgca
     1441 cgaaagcagc tcgtccagta cctcaagttt catgagatga tctgtgagaa acccgagttt
     1501 ctcgggatga gcaatcatct tttatacatc ggtagaaagt agcacggagc aaaaagcttc
     1561 aaatcagcgt aatggaggca cagagtatgt cgagaatgcc tgtggtgttt gtaggacacg
     1621 gttccccgat gaatgctatt gaagacaacg cttactctag aggttggcga gagatggcca
     1681 aacggatccc aaaaccaaaa gcgattgttt cggtgtcggc tcattggtat actagaggaa
     1741 ctagaatcat ggatgaagaa aaccccgaag ctatctatga catgtacggt tttcccaaag
     1801 agttatatga ggttacatac aacgcgcctg gctcgccgga aatcgcaaag gccgttaagg
     1861 aactgatctc gagagaaatc cagttcgaca actcctgggg aattgatcac gggacctggt
     1921 cggtcctagt gcatatgtat ttacaagtac agcttcagcg acagaaacat catttaggtt
     1981 tgattgtaat cagggatcat ctggctccgc aattaatgtt tctaactcat catctaatgt
     2041 ccaagctact tggcgcgtta atgcctccgg aggctgggat gcgtatgtag ggcaggcagc
     2101 cgccgcagca aacactaccg gtactg
//
LOCUS       DAOSDA010000310         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_34436_length_2121_cov_2.968538, whole genome shotgun sequence.
ACCESSION   DAOSDA010000310 DAOSDA010000000
VERSION     DAOSDA010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34436_length_2121_cov_2.968538"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PLW82_07135"
     CDS             complement(<1..882)
                     /locus_tag="PLW82_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47084.1"
                     /translation="MKRKRASQRSFFLIECFKTPRDAATTLFAALSIILYASCLVSLV
                     FLSDQIVVAYTRGEPLDLFFTYILILLAFSRLISLFTSVFQQSLTGNVFYRKIKNLLE
                     SVSPKLLRDKDFSFKEIYRGLEMRTELRSRCFFEFLQGLFGAAFLGLAISYFAPWAVL
                     VLIVGSLLAFYFTAPLAVAHEHQGQGSTLQVSPDWLFEAEHYGAKEFFFGEIKKLYAE
                     ENQRSAKFQMVINFLASSGRVIEAGAFLLSLGMSTYYLAQAQATSGSVLIVLIFGRSL
                     QKHLGQILTSIKQVLRRD"
     gene            complement(888..>2121)
                     /locus_tag="PLW82_07140"
     CDS             complement(888..>2121)
                     /locus_tag="PLW82_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ47085.1"
                     /translation="LVSWWFLFSTARAVKAEVQKTTKDSTLKERNLALLTESGFIFEK
                     AIYNLEKYLMSGFTRSYFRSQKQKIVMTFTENFRNAAVFICYSFALFVSLLISAKVYK
                     GGITLGALFGLAFSAKLMWEFLERSASQVGSAFGTKIDLPADFFEVVPGASKDTDGST
                     VSIDLSELVGFEIRDLSVQLGGKRVLEEVSIKIRPGEKVAVVGERGSGKSTLLGAISG
                     RYEQVFGEVSLGGIPTSLLGDRLSRFIGAVFEDPMVFPVSIKENITLDKKSQPDVDRK
                     LQNASLKSGLEETLRIFPRGMETRLSSEILSKFREEDRKLLVKKIALARALYRDPAIV
                     FIDDVPFGSDKEQLWLLKFLEKYEKTCVWFSENPYAGQPADKVVFLRDGRVVEEGSFD
                     QLVESGGEFAKFVFGFRG"
BASE COUNT          570 a          558 c          471 g          522 t
ORIGIN      
        1 gtctctacgc aacacctgtt tgatggatgt gagaatctga ccgaggtgtt tttgcagact
       61 cctaccaaag atgagcacta taaggacgct tccggaagtt gcttgagctt gagccaggta
      121 gtaagttgac atgcctaggc tcaggagaaa agcgcccgct tcgatgactc tacccgatga
      181 ggcaagaaag ttaatcacca tttggaactt ggcagacctt tgattttctt ctgcatatag
      241 ctttttgatt tccccaaaga aaaactcttt ggccccgtag tgctccgctt caaatagcca
      301 atcgggactt acctggagtg tggatccctg gccttgatgt tcatgtgcaa cagccagcgg
      361 tgcggtgaag taaaacgcaa gaagcgaccc tacgatgaga accagcactg cccagggagc
      421 aaagtacgaa atggcaaggc ctaggaacgc tgctccgaac agtccctgta aaaactcgaa
      481 gaaacaccgg ctgcgaagct ctgtgcgcat ctccagccca cggtagatct ccttgaagga
      541 gaagtccttg tctctgagga gcttcggcga gacactctcc aacagattct tgattttgcg
      601 atagaaaaca ttgcctgtaa gcgactgctg aaaaaccgac gtaaacaaac tgatcaaccg
      661 ggaaaaagca aggaggatta gtatataggt gaaaaacagg tccagaggct cgccccgtgt
      721 ataggctaca actatttgat ctgaaagaaa cactagggat accaaacagc tggcataaag
      781 gatgatggac agtgctgcaa acagcgttgt agcagcatct ctcggcgtct tgaaacactc
      841 aataagaaaa aaactcctct ggcttgctct ctttcgcttc actgctttca ccccctaaac
      901 ccgaaaacaa acttggcaaa ctctccaccc gattccacca gctggtcgaa agagccttcc
      961 tccacaactc tgccatctcg aagaaagacc actttatccg ctggctgacc tgcatacgga
     1021 ttctcgctaa accacacgca ggtcttctca tatttttcca agaacttcag taaccacagt
     1081 tgttccttgt cgctgccaaa cggtacatca tcgatgaaca ctatagccgg atccctgtag
     1141 agggctcgtg ctaaagcaat tttcttgact aggagctttc tgtcttcctc tctgaacttt
     1201 gacaggatct ctgaagacag tctcgtctcc atgcctcggg ggaagatgcg cagcgtctct
     1261 tccagccccg atttcaagga agcattctgc agcttgcggt cgacatccgg ttgcgacttt
     1321 ttgtccagag taatgttctc cttaatactt acagggaaaa ccatgggatc ctcaaaaacg
     1381 gctccgataa accttgaaag cctatctccg agtaagctgg ttggaattcc gccaagacta
     1441 acctcaccga agacctgctc gtatctgccc gaaatggctc cgagaagagt tgactttccc
     1501 gaaccgcgct cgccaacaac tgcaactttc tctccaggac ggatcttgat actgacttct
     1561 tctagcactc tttttccgcc gagctgcacc gataaatccc tgatctcaaa acccaccagc
     1621 tcgcttaagt cgatggaaac ggtagagcca tctgtgtctt tgctcgcacc tggcacgact
     1681 tcgaaaaagt cagcaggtag gtcgatcttt gtgccgaaag cactgcccac ctgacttgcc
     1741 gacctctcca gaaactccca catcagtttt gcgctaaaag ccaggccaaa tagcgctcct
     1801 agagtgatgc cgcctttata gacttttgca gaaatcagta gcgatacaaa aagtgcaaac
     1861 gagtagcaga tgaaaactgc tgcgtttcgg aagttctcgg taaacgtcat aacgattttc
     1921 tgcttctgac tcctgaagta gcttctggtg aagccggaca tgaggtactt ttctagattg
     1981 tagatcgctt tttcgaagat aaaacccgac tctgtaagca aagcgaggtt tctttcttta
     2041 agggtgctgt cttttgttgt cttctgaact tctgctttaa ccgctcttgc ggtgctgaaa
     2101 agaaaccacc aagaaacaag c
//
LOCUS       DAOSDA010000311         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_34487_length_2119_cov_2.612403, whole genome shotgun sequence.
ACCESSION   DAOSDA010000311 DAOSDA010000000
VERSION     DAOSDA010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34487_length_2119_cov_2.612403"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLW82_07145"
     CDS             complement(<1..596)
                     /locus_tag="PLW82_07145"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HOJ47086.1"
                     /translation="MKRIILGLLVFCLLAEVVCAFSYTGSQGLLTLPTANMRRNGSGS
                     IGLNYAENDISGALAATFLENLELGIAAGSRDPVYGFFKWRLSEETLDRPAFAVGASG
                     SSFYAVLSKNLLPNGLKAHIGMGSGEYKGVFGGISYVVNPVSISPRKLPVPIVTLLLE
                     LKDNSREQEGSFVLNFGGRLQLNDYFSVDIGLLDMKTLA"
     gene            complement(602..1660)
                     /gene="lpxD"
                     /locus_tag="PLW82_07150"
     CDS             complement(602..1660)
                     /gene="lpxD"
                     /locus_tag="PLW82_07150"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOJ47087.1"
                     /translation="MKLSQKKPPITLGEFHRMIGGELIGDPEVLISHTAPIETAGEGA
                     MTLVAGRSAKEKAKDNLATAYIIERKFADDLEVNGIIVDDGREAFAVVLGYFQRKVIH
                     TGTVSSKAVVELGAKIGNNVTIYDFAYIAADAEIGDGTVIFPHVFVGPGSKVGRDCVI
                     YPQVVIRENVTIGDRAIIHSGVIIGVDGFGFTLTEEGWKKVPQIGGVVIGNDVELFSN
                     TSIASGAAGPTVIADGVKVGDMTHVGHNSVIGRDSIMAGQIGISGSTVIGKRVMVGGQ
                     VMFAGHQTIGDGAGIMSKALVDGDIEPGAIVSGIPARDNKKELRIKASMQDLPELRKQ
                     VKELAKKLDEVLAKQKEN"
     gene            complement(1684..>2119)
                     /locus_tag="PLW82_07155"
     CDS             complement(1684..>2119)
                     /locus_tag="PLW82_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47088.1"
                     /translation="NLERERRLVELESQEQERLEKELEELEARYRLQEETIKEQMAEA
                     AQGVEVRRNNMSETFSHLESQTLTQNNLLISSYRQLQEKQLNDLIVRKDNLLRNIESE
                     CSQTLRLLAMRDGYEPGGLVETVPEGAVDITDMILDAHRKTE"
BASE COUNT          459 a          585 c          489 g          586 t
ORIGIN      
        1 gcaagtgttt tcatgtctag caggccaata tcaacactga aatagtcgtt aagttggagc
       61 cggcctccga agttcaagac aaaggagcct tcctgttccc ggctgttatc tttaagttcg
      121 agaagcaaag ttacaattgg tacgggaagc tttctcggtg aaatggacac gggattaacc
      181 acgtaggaaa ttcctccaaa tactcccttg tactcgccgc tacccatacc gatgtgggct
      241 ttaagaccgt ttggcagtaa gttcttgctg agtactgcat agaaactaga tccagatgca
      301 ccaactgcaa aagcgggtct atccaaggtt tcctctgaga ggcgccattt gaaaaaacca
      361 taaactggat cgcggcttcc tgcagctatg cccagctcta aattctctaa aaacgtggcc
      421 gccaaggccc cggagatatc gttttcggcg tagttgagac cgatgctccc gcttccgttt
      481 ctgcgcatgt tggcagtggg aagggttaac aacccttgcg acccggtgta gctgaaagca
      541 cacactacct ctgccaagag gcagaaaact aggagtccta gaataattcg cttcactatc
      601 tctaattctc cttttgcttc gccaaaacct catccagttt tttcgccagt tctttcacct
      661 gcttgcggag ttccggcagg tcctgcatgc tggctttgat gcggagttct ttcttgttat
      721 cccgtgcagg gatgccgctg acgattgctc caggttcgat atcgccatcc acaagggctt
      781 tgctcatgat accggctcca tccccgatgg tctggtgccc ggcaaacata acttggccgc
      841 caaccatcac tcgcttgccg ataacagtgc tgccgctgat gccgatctgt cctgccatga
      901 tgctgtctct accaatgaca gagttgtgac ctacgtgggt catatcgccc acttttacgc
      961 cgtctgcaat tacagttggc cccgcagctc ctgaagcaat agaggtgtta gagaataatt
     1021 cgacatcgtt gcctataact actcctccga tttgtggcac ctttttccaa ccttcttcgg
     1081 tgagggtgaa gccaaatccg tcgacgccaa tgatcacgcc tgaatgaatg atggcgcggt
     1141 cgccaatggt gacgttctct ctgattacca cctgcggata gatcacgcaa tctctgccaa
     1201 ccttgctacc aggcccaaca aaaacgtggg gaaagatcac tgttccatca ccgatctctg
     1261 catcggcagc aatgtaggca aagtcgtaaa tggtgacgtt attgccaatc tttgccccca
     1321 gctctactac agctttactg cttacggttc cggtgtggat gacctttctc tggaaatacc
     1381 ccaagacaac agcaaaggcc tcgcgtccgt catcgacgat gatgccgttt acctcgaggt
     1441 catctgcaaa cttcctctcg atgatgtaag ctgtagcgag attatccttg gccttttcct
     1501 tggcggagcg tccggccaca agggtcattg ctccttctcc tgcagtttca ataggtgctg
     1561 tatggctgat cagcacctca gggtctccga taagctctcc cccaatcatt cggtggaact
     1621 ctcccagcgt gattgggggc tttttttgtg ataacttcaa cgaaactgcc cccccctagc
     1681 ttcttactct gtttttctat gcgcatcaag gatcatgtcc gtgatatcaa ctgcaccttc
     1741 aggtacggtc tccacaagtc caccgggttc gtagccatcc ctcatcgcca aaaggcgaag
     1801 agtctgactg cattcgcttt cgatgttcct taggaggttg tcttttctga cgatcagatc
     1861 gtttagctgc ttctcttgga gctgcctgta cgacgaaatc aggaggttgt tttgggttaa
     1921 cgtctggctt tccagatggg aaaaagtctc agacatattg tttctgcgga cttcaacccc
     1981 ttgagctgct tcggccatct gttctttgat ggtctcctct tggagcctgt atctggcctc
     2041 aagctcttct agttccttct ccaggcgttc ttgttcttga ctttctagtt ccacaagcct
     2101 gcgctcgcgc tcgaggttg
//
LOCUS       DAOSDA010000312         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_34507_length_2118_cov_2.419777, whole genome shotgun sequence.
ACCESSION   DAOSDA010000312 DAOSDA010000000
VERSION     DAOSDA010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34507_length_2118_cov_2.419777"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLW82_07160"
     CDS             <1..353
                     /locus_tag="PLW82_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOJ47089.1"
                     /translation="EQVVRYATSIVPREKVVLGLPFYGRHWTTNSRGEHSAGRGVTYE
                     QAMAFADKWQVEPVLHEHGCMYLRKEHKDGSVEEIYFENKDTLLKKINLAEGLAGVAF
                     WRLGQEDPRIWEYL"
     gene            complement(676..>2118)
                     /gene="nagZ"
                     /locus_tag="PLW82_07165"
     CDS             complement(676..>2118)
                     /gene="nagZ"
                     /locus_tag="PLW82_07165"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOJ47090.1"
                     /translation="GGVIYFSRTIESPSQVRNLSETLQKLSSVPLLICTDQEGGTVMR
                     IVNGVTVFPGNMALGATRSTAMAYQAGKITGEELYMLGINMNLAPCLDVNNNPKNPVI
                     GLRSFGEDPELVADLGSAFALGLQDAGVIATGKHFPGHGDTEIDSHHLLPVINHDRNR
                     LEAVEFAPFKKAIVQGIGAIMTAHIAFPALEGNTEVPATLSRKVLTDLLRNELGFDGL
                     IVTDCMEMKAIKDTFGTAEGAILAVQAGADIILVSHTRSVQLEVYNALLAAVKEKRIS
                     EDDLDQKVLRILKAKFDARIMSQNWTESKLTAPSPENLRVAGDMAQSAVVVLKGAELL
                     PLPGRAVVVDFETSARSIAEDVIKGHVYLGDLAPEGSVGVRIPLGGQDIREFLALLKD
                     QKYDALVVGTLDADRNPYQAELVAQLKTLGKPMVAVGLRAPYEFSMLKDLVDSYIVTF
                     SHRKPSLEAAWNVLLGKSKAPGILPVSLPE"
BASE COUNT          535 a          525 c          545 g          513 t
ORIGIN      
        1 tggagcaagt ggtaaggtat gctaccagta tcgttcccag ggagaaggtc gtcctcggtc
       61 tgccattcta cggacgccat tggaccacga actcaagagg cgagcactct gcaggcagag
      121 gggtaaccta tgagcaggcc atggcttttg ctgacaagtg gcaggtagag ccggtacttc
      181 atgaacacgg ctgcatgtat ctaaggaagg aacacaagga tggctccgtg gaagagatct
      241 acttcgagaa taaagacact cttctcaaaa aaattaacct tgcagaaggc cttgcaggag
      301 tagctttttg gcgcttgggt caggaagacc cacgcatttg ggagtatctt taatcagctc
      361 gaaaggtgcc ttgaagatca cttcggggta cttttgtatg accgcacccg cagctgcgag
      421 gaaagaaggg ccgctttagg tctactaacc tgactgcata actaaaactt gtaatgagtc
      481 aagcgacata tcccacgctc cacagtcaaa atttgacatt agtgtttcag aaaaaggcac
      541 tctacgttgt ggtattcgta gagtgccttt tcgataataa ggtcaagcgg acgatcacat
      601 atccaaggga aactgtaagc ttataaaagc gagctgctgg attggctccg gcagctcaaa
      661 aaacttgtaa actgtttatt cgggcaaact cacaggaagg atgcccggag ctttggattt
      721 accaagaaga acgttccaag cggcttcaag agagggtttt ctatggctga aggtgacaat
      781 gtaagagtca acaaggtcct ttagcataga gaactcgtac ggagctctga gcccaacggc
      841 aaccataggt tttccaagag ttttaagctg tgcaaccagc tcagcctgat atggattgcg
      901 atctgcatcg agggtcccaa caaccaaagc gtcgtatttc tggtctttca agagggcaag
      961 gaactctctt atatcttgcc cgcccagcgg aatgcggaca cccaccgagc cttcaggggc
     1021 aagatctcca agataaacgt gacctttgat aacgtcctca gcaattgatc tcgcacttgt
     1081 ttcgaaatcg accaccacag ctctgcccgg aagaggcagg agttctgcac ctttcagaac
     1141 gaccacagcg ctttgagcca tgtctccggc cacccggagg ttttccggcg aaggcgctgt
     1201 cagcttgctc tcggtccagt tttggctcat aatgcgggca tcaaacttag cctttagaat
     1261 ccgcaggact ttttgatcca ggtcatcttc gctgattctc ttctctttga ccgccgcaag
     1321 aagtgcgttg tagacctcaa gctgaacaga tcgagtatgg ctcacaagaa tgatatcagc
     1381 gccggcttga actgcaagga tcgccccttc tgcagtacca aaggtatctt taatagcctt
     1441 catttccatg cagtcggtga caatcagtcc gtcaaagccc agctcattcc tgagtaggtc
     1501 cgtcagcact ttccgggaaa gtgtggcggg gacttcggtg tttccttcta aggcgggaaa
     1561 ggcgatatga gctgtcatga tggcgccaat cccttggaca attgcctttt taaacggagc
     1621 aaactcaacc gcttccaaac ggttcctgtc gtggttgatc acagggagaa ggtgatggct
     1681 gtctatttcc gtgtctccgt gcccggggaa atgtttgcct gtagcgataa cgcctgcatc
     1741 ttgaaggccc agggcaaaag ccgagcccag gtcggccaca agctccggat cttccccgaa
     1801 agatctcaag ccgatcaccg ggtttttcgg gttgttgttg acatcaaggc agggggcaag
     1861 gttcatgttg atacccagca tatacaactc ttcgccggta atcttgcctg cttgatatgc
     1921 catagctgta gaccttgtgg cacctagtgc catgttgcca gggaaaacgg taacgccgtt
     1981 aacaattctc atcaccgtgc cgccttcctg atctgtgcag atcagaaggg gaacggaact
     2041 gagcttctgg agagtctcgc ttaggtttct aacctgagag ggactttcaa tggtacggct
     2101 gaagtaaatg actcctcc
//
LOCUS       DAOSDA010000313         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_34586_length_2114_cov_2.788732, whole genome shotgun sequence.
ACCESSION   DAOSDA010000313 DAOSDA010000000
VERSION     DAOSDA010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34586_length_2114_cov_2.788732"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="PLW82_07170"
     CDS             <1..1274
                     /locus_tag="PLW82_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HOJ47091.1"
                     /translation="NIPLAMSAQFLGLLTSGFFIKSAARAKLTMLYGTGIAFITLIPF
                     FFAPSLLWKVGLLLGGYSSGAAIAAWGYFLKIFTERKERLKTCADVLITSNIFMIVIN
                     FIAIRLTPYVGLVIVMLALIIGMLLTGRLKVDAALDGVTKKGSVQSTGRLKNAVLVLC
                     LFIAVLTINSGIMYVVINPAFEHLTSLVSWYWAVPYIIALFVMRSLPWASRHSRVLYL
                     GMAMMIGAFIAFMLLGRASLDYLVVNTLFLGACGFFDLFWWSILGNMLDYTEKPSFVF
                     GIGLSANVLGVLAGSMIGRVARSTHLLTAEITVMGLTVVCVTLGVLPILNRYLVLLLK
                     DQPYLLAFEAQDASTERSASYQAEALDPLTSREREVLEHLLAGKSNQEIAGALSVTVN
                     TVKTHAKNIYSKYDVHSRAELISLLLKGRDS"
     gene            1794..>2114
                     /locus_tag="PLW82_07175"
     CDS             1794..>2114
                     /locus_tag="PLW82_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47092.1"
                     /translation="MLLLLVISLLSGCLDGILNNKKTYKLTVTVLEEATDNPIQGAVV
                     EIVGKVGAHQETDKDGKANLVGLSETLELNIEAPGYLPQSKSVVMNKDQSIVVKLAIN
                     ATGTV"
BASE COUNT          529 a          433 c          530 g          622 t
ORIGIN      
        1 gcaatattcc actggcaatg agcgcccaat ttctcggcct tctgaccagt ggttttttta
       61 ttaagtctgc tgctagagcc aagttaacga tgttgtacgg aacgggtatc gcttttatca
      121 cattgattcc attctttttc gctccgtctc ttttgtggaa agtgggacta ttacttggcg
      181 gttacagcag tggtgccgca atagctgcct ggggttactt cctgaagata ttcacagaga
      241 gaaaagaacg cttaaaaaca tgtgctgacg tactcattac gtcaaatatc tttatgatcg
      301 tcatcaactt catcgcaatc cggctaacgc cgtatgtcgg attagtgatc gtgatgttgg
      361 ctttgatcat tggaatgctt ttgacaggca ggcttaaagt tgacgcggct cttgatggtg
      421 ttacaaaaaa gggcagtgtt cagtctacag gcaggttaaa gaatgccgtt ttggttctct
      481 gtttgtttat cgcagtactc accattaact ccggcattat gtatgtggtt atcaatcctg
      541 catttgagca cttaacctct cttgtgagtt ggtattgggc cgttccgtat atcatcgccc
      601 tttttgtcat gcgcagtcta ccatgggcaa gtaggcattc gagagtgctt tatctcggaa
      661 tggccatgat gataggtgcg tttatcgctt ttatgctcct tggcagagcc tcactggact
      721 acttagtggt aaacacctta tttctaggag cttgtggttt ctttgatctc ttctggtgga
      781 gcatcttagg gaacatgcta gattacaccg aaaagcctag tttcgtcttt ggcattggct
      841 tatctgctaa tgtccttggg gtcttggctg gaagtatgat cggtagggtg gcgaggtcaa
      901 ctcacctgct cactgcagaa atcactgtta tgggccttac cgtcgtctgc gtaaccttag
      961 gcgtccttcc gattcttaac cggtacctgg ttcttttgct gaaggatcaa ccctaccttt
     1021 tggcttttga agcccaagat gcaagtaccg aacgttctgc ttcgtatcaa gctgaagctt
     1081 tagatccgtt gactagtaga gaacgggaag ttttggaaca tttgcttgca ggcaagtcca
     1141 atcaggaaat agcaggtgcg ttgtctgtca cagtcaacac ggtaaaaaca cacgctaaga
     1201 acatctattc taagtacgat gttcatagta gggcggagtt aatcagcctg ctcttgaagg
     1261 ggagagactc ctgatataac ctactggttc tttcgctaga cgcaggagag gttatagacc
     1321 tttctttccc attatgtagg actgagtgaa atacaatctg ccccttatgc tggtctttta
     1381 aagcctccta cccacaatgg gtgagggggc tttttgtcga tttcatccgt atggtgcacg
     1441 acctggcagg taattactgt tacgataaaa tcgcatagtg gacccatcgg tgttctaggg
     1501 gtcgaaaagt ccgcgatgta gacaaagtag tcgggtttgg cgaggggtac gctgtctttt
     1561 actacagatc gacttaaaac ggcgtttgcc ctgagatttg gaagagaggg gtgtcggaac
     1621 aaacaagggg ggggagagtg tatccccgcg tgttgggaca gcttactaga gtttgcaccg
     1681 gtaaagtgtg gtgctttgct cttgttgaac gtagtgcttt gacatagggc ttaagacgag
     1741 atcaaaaaaa aggagctaaa aacatgaaaa gatcacacag acttgcacag tggatgttac
     1801 tgttactagt cattagtctt ttatccggct gtctagacgg tatcttgaac aacaagaaga
     1861 cttacaagct cactgtaaca gtgttagaag aggccacgga caatcctatc caaggagctg
     1921 tggttgaaat tgtaggcaag gttggtgccc atcaagagac agacaaagat ggaaaggcta
     1981 atcttgttgg gttgtcggaa accttagaac ttaacattga agctccaggt tatctgcctc
     2041 aaagcaaatc tgtagtcatg aacaaagatc aaagcattgt tgttaagctt gctattaacg
     2101 cgacgggaac ggtt
//
LOCUS       DAOSDA010000314         2092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_35106_length_2092_cov_3.395680, whole genome shotgun sequence.
ACCESSION   DAOSDA010000314 DAOSDA010000000
VERSION     DAOSDA010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35106_length_2092_cov_3.395680"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1119)
                     /locus_tag="PLW82_07180"
     CDS             complement(202..1119)
                     /locus_tag="PLW82_07180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOJ47093.1"
                     /translation="MREELYRNKRWSSRNKTLYMEPGDEKIYIPKPLPEGPIPPGIYM
                     GEGLMALKRIESDSVQAVFADPPYFGKQKAFGRSVSNVWEDYSAWTSAWLAEAYRVLK
                     KTGSIYVCCDWSYSGKIQELMEAAGFKVLNRITWKRDKGRGAQKNWKNNMEDIWFAVK
                     DEKEYYFNVESVKIKKKVIAPYRDSKGKPKDWVEEDGDRYRYTYPPNIWIDITVPFWS
                     MAENTPHPTQKPEKLVDRCLRPVLQPGDLVVDPFLGSGTTAIIADRIGAECIGIELDP
                     DYVCLAMKRWELRHEIAAKSSLDFEMEIE"
     gene            complement(1139..>2092)
                     /locus_tag="PLW82_07185"
     CDS             complement(1139..>2092)
                     /locus_tag="PLW82_07185"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOJ47094.1"
                     /translation="MSLVHKKMKIFSGNANPALAREIASNLGMNLGDSSVTRFKDEEI
                     SVNINETVRGADVFIIQPTSKPVNENLMELLIMIDACRRASAKSITAVIPYYGYARQD
                     RKTKPREPIAAKLVANLITAAGANRVLTVDLHAGQIQGFFDIPVDNLKALPILANYFI
                     KKGLRGDDVVVVSPDVGGVTRARELAGILGTNLAIIDKRRPKANVAEVMNIIGDVKGK
                     TAIMIDDMIDTAGTITQGAQAILDAGAVDVMACCSHAVFSKPAIERLRDSVLSEVVVT
                     NSVPLSEEGAAVDKIKVLSIASLLAEAMRRIYEELSVSVLF"
BASE COUNT          493 a          531 c          464 g          604 t
ORIGIN      
        1 cgttgctgtt acaacggatt ctgagctttt ttcatctttt tactgtcaaa gacggaagta
       61 tatcaccaat cgataccttt ataaacccat ttttagagca gccaaagctg tggccaactc
      121 atactagtgc ctagctaaag ccaaaatcct ctagggacac aataagtccc tcgacgaggg
      181 acttattgtt gcatctttga attattctat ctccatctca aaatccaaac tggattttgc
      241 tgcaatttcg tggcgaagct cccagcgttt cattgctaag catacgtaat cgggatccag
      301 ttcgatccct atgcactctg cacctattct gtcggcaata atagctgttg tccccgaacc
      361 gagaaacgga tccacaacca agtctcccgg ctgcaggaca ggtctcagac atctatcaac
      421 aagtttttcc ggtttttgcg tcggatgagg tgtgttttcc gccatagacc aaaacggcac
      481 cgtaatgtct atccagatgt tgggaggata ggtgtagcgg tagcggtctc cgtcctcctc
      541 cacccaatct ttaggcttgc cctttgaatc cctgtacgga gcgatgacct tcttcttgat
      601 tttaacgctt tctacattga agtagtactc tttctcgtct tttaccgcaa accagatatc
      661 ttccatgttg ttcttccagt ttttttgagc tccgcgccct ttgtctctct tccaggtgat
      721 ccggttgagg accttaaacc ctgcagcttc catcagctct tgaatctttc ccgagtagct
      781 ccagtcgcag cagacataga tggagccggt ttttttcaag accctgtaag cttcggcaag
      841 ccatgctgaa gtccatgcgg aataatcttc ccaaacgttg gatacgctgc gtccaaaggc
      901 cttttgcttt ccaaagtaag gcggatctgc aaagactgcc tggactgaat cactttcaat
      961 ccgcttcaga gccattagcc cttctcccat gtagatccct ggaggtatag gtccttctgg
     1021 cagtggcttt ggtatataga ttttttcatc gcctggttcc atgtagagtg ttttgttcct
     1081 gcttgaccag cgtttgtttc gatatagttc ttctcgcatg atttcctcct aatgtttcct
     1141 aaaagagcac actgaccgag agttcttcgt aaatgcggcg catagcttct gccagcaagg
     1201 atgcgatcga cagtacctta atcttatcaa ccgccgcacc ttcctcagat agaggcactg
     1261 agttggtcac taccacttca gaaagcacag agtctctgag cctctcaatg gcaggtttgg
     1321 aaaatacagc atggctgcag caggccataa catctactgc tcctgcatct aagatagcct
     1381 gtgcaccttg ggtaatggtt ccggcggtgt cgatcatgtc gtcgatcatg attgcggtct
     1441 tccctttaac atcgccaata atgttcataa cttcagccac attggctttg ggacggcgct
     1501 tatcgataat agcaaggttt gtcccaagaa tacctgcaag ttctctggcg cgggtcacac
     1561 cgcccacgtc aggagacacg actactacat catcgcctct aagacctttc ttgatgaagt
     1621 agttggcaag gataggcagt gctttaaggt tgtccacagg aatatcgaaa aagccttgaa
     1681 tctgacctgc atggaggtcc acagtgagca cgcggttggc tccggcagca gtgattagat
     1741 tagccacaag tttggccgca atgggctcgc gaggcttggt cttgcgatcc tgccgtgcat
     1801 agccgtagta aggaataacc gctgtgatgg attttgcaga tgcccttctg caggcatcga
     1861 tcatgatcaa gagctccatc aagttttcgt ttacgggttt tgaggtaggt tgaatgataa
     1921 aaacatctgc acctctaacg gtttcgttaa tattaaccga tatctcttcg tctttgaaac
     1981 gcgtaacaga ggagtctcct aagttcattc cgagattgct ggcaatctct ctggcaagcg
     2041 cagggttggc gttgccggaa aaaatcttca tctttttatg caccaacgac at
//
LOCUS       DAOSDA010000315         2092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_35112_length_2092_cov_2.835542, whole genome shotgun sequence.
ACCESSION   DAOSDA010000315 DAOSDA010000000
VERSION     DAOSDA010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35112_length_2092_cov_2.835542"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLW82_07190"
     CDS             <1..269
                     /locus_tag="PLW82_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47095.1"
                     /translation="RYYLEPSTMKSIFAQTGAYALGAVVGSNFYLTTGISIEGGYKYV
                     MDNGITAEASAGLSTGILSINSVTGLGFPIRPGFVFGVGVGYSW"
     gene            complement(300..1520)
                     /locus_tag="PLW82_07195"
     CDS             complement(300..1520)
                     /locus_tag="PLW82_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ47096.1"
                     /translation="MWLIEALFISFKSLGENKLRTFLSVLGIVIGIASVIAMMSLGDG
                     VTEQVLRSISSLGSNVITISRRYTVARRGRSSATAADRVFTLDLAARLKEAVPELIDV
                     VPSVETNATLYLDTGDSLQTRVIGATPGYVDVYEYPLLVGRFIDEDDLAENRLVAVLS
                     LETATELFGLTNPIGQSIRANIGNHSVKLTVVGVMSKKPQTAITFASNQIFVPVTALT
                     SRGIASKSISSFVTKYESSDEEQHVMAALNHYLTSRLGSSNEFSVVSQESIRETMNEV
                     ASTITWFLAGIGGIGLLVGGIGIMNIMLVSVTERTREIGIRKSLGAKKKHIFMQFLLE
                     ALVLSVAGGIIGIGAGKLLGDLMTKIIQIKFIFSVRATVIAILFSSAVGLFFGVYPAQ
                     KAARLNPVDALRYE"
     gene            complement(1507..>2092)
                     /locus_tag="PLW82_07200"
     CDS             complement(1507..>2092)
                     /locus_tag="PLW82_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ47097.1"
                     /translation="MGSSGSGKSTLMHIMGCLDEPTSGSIAIDGRDVKQFSDSALAEL
                     RNTKIGFVFQSFNLLPRLNILQNVEVPLQYRGVSRKARRKLAEEALAAVGLSGRLKHL
                     PSEISGGERQRVAIARALVNNPTIILADEPTGNLDSKTGTAIMDLFKDLNKKGHTIIL
                     VTHSREVAEYAQRIIHLKDGLIERVEEGGLGVVN"
BASE COUNT          501 a          539 c          507 g          545 t
ORIGIN      
        1 ttcgctacta cttagagccc agtacgatga agagcatttt tgctcaaact ggcgcttacg
       61 cattaggtgc cgttgtgggt agcaacttct accttactac tggaatcagt atcgaaggcg
      121 gttacaagta cgttatggat aacggaatta ccgcagaagc ctctgcagga ctttccactg
      181 gaatcctctc catcaactct gttactggac tcggattccc cattcgtccg ggcttcgtct
      241 tcggtgttgg cgtaggttat tcttggtaag acaaaaaaga gggcacatgc cctctttttt
      301 tattcatatc tcaaagcatc tacaggattg agtctggctg ctttttgcgc cggatagact
      361 ccaaagaaga ggcctacagc actggagaac aggatggcaa taaccgtcgc cctcacagag
      421 aagatgaact tgatctgaat gatcttggtc atcaggtcgc caaggagctt cccggctcct
      481 ataccgatga tgccgccagc aacgctcagc acaagtgcct ccagtaaaaa ctgcatgaaa
      541 atgtgctttt tcttggctcc caaagacttg cgaatgccga tttctctagt tctttcggta
      601 accgagacca gcatgatatt cataatgcca atgcctccga ccaaaaggcc tattccgccg
      661 ataccggcca aaaaccaggt aattgtactg gcgacctcgt tcatggtctc gcgaatactc
      721 tcctgagaga ccacgctgaa ctcgtttgaa ctgcccagtc tgcttgtgag gtaatggttc
      781 agtgctgcca tgacgtgctg ttcctcgtcg gagctttcgt actttgtaac gaagctggaa
      841 atggattttg aagcaatccc cctgcttgtt aaggcagtta cgggaacgaa gatctggttg
      901 gaagcaaacg taatggccgt ttgcggtttt ttgctcatta cccccacaac tgtgagcttg
      961 acgctgtgat tgccaatgtt ggccctaata ctctggccta taggattagt tagaccgaag
     1021 agttccgtcg cggtttcaag gctcaaaacc gcaaccaacc ggttttctgc cagatcatct
     1081 tcgtcgataa acctgccaac cagaagagga tactcataaa cgtctacata ccctggggtg
     1141 gccccgatca ccctggtctg caggctatca ccggtgtcga ggtataaggt ggcgttggtc
     1201 tcaacagacg gaacaacatc gattagctct ggaacggctt ctttgagcct tgctgctagg
     1261 tctaaggtga aaaccctatc tgctgcagta gcactagagc gccctctccg cgcaactgtg
     1321 taacgcctgc tgatggtgat tacgttcgaa cctagagaac tgatggagcg caagacctgt
     1381 tcagttacac cgtcgcctaa ggacatcata gcgattacag acgcgatacc gatgacgatg
     1441 ccgagaactg aaaggaaagt gcgcagcttg ttttcgccaa gactcttaaa agagatgaat
     1501 agagcctcaa ttaaccacac ctaaaccacc ttcctctacc ctctcaatga gcccgtcttt
     1561 caagtgaata attctttggg catactctgc cacttctctg gagtgggtca ctaagattat
     1621 tgtgtgccct tttttattaa ggtccttaaa caggtccatt atggccgttc ctgttttgga
     1681 gtccaggtta cctgtgggct catcggcaag aataatggtg gggttgttta ccagagctct
     1741 agctattgcc actctctgtc tctcaccacc ggagatctcg ctggggaggt gcttcagcct
     1801 tccggaaaga ccaacggcgg cgagagcttc ttccgcaagc ttcctacggg ctttacgact
     1861 tacccctctg tactgcaggg ggacttcaac gttctgtagg atgtttagcc tgggcagaag
     1921 gttaaaactt tgaaagacaa agccgatttt tgtgtttcgc agctctgcca gagcactgtc
     1981 ggaaaactgc tttacgtccc tgccgtcaat ggcgatggaa ccggacgtgg gttcgtcgag
     2041 gcagcccatg atgtgcatca gcgtggattt ccccgaaccg gatgacccca tg
//
LOCUS       DAOSDA010000316         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_35173_length_2089_cov_3.605211, whole genome shotgun sequence.
ACCESSION   DAOSDA010000316 DAOSDA010000000
VERSION     DAOSDA010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35173_length_2089_cov_3.605211"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLW82_07205"
     CDS             complement(<1..325)
                     /locus_tag="PLW82_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOJ47098.1"
                     /translation="MNILVINSGSSSLKYKLFDMDKKVPVVSGIAERIGFDGSFIKHE
                     NGEKTVINIELPDHKVALQEVLKLLMDPKLGAIKSLDEIDAVGHRILHGQEVFTESVI
                     LDKEKV"
     gene            576..1091
                     /locus_tag="PLW82_07210"
     CDS             576..1091
                     /locus_tag="PLW82_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOJ47099.1"
                     /translation="MITIKTTRNLITASLLAATAIVLTRFFGIPLGTVRISLGTIPIM
                     LSGIVLGPFWGLATGTVADLVGVLINPQGGAYFPGFTLTTALVGFLPGFILRNKRPPL
                     WKLAVAIGVTELLCHVFLNTLWVGMITGKAYLALLPSRIISRVVHFPIITGAIYFLIQ
                     KVFAPKKTTED"
     gene            complement(1088..1549)
                     /locus_tag="PLW82_07215"
     CDS             complement(1088..1549)
                     /locus_tag="PLW82_07215"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOJ47100.1"
                     /translation="MEIALFEPEIPQNTGNIARLATASGLELNLVGALGFHLSDKLVK
                     RAGMDYLDIDSIKRTATLEELYQSKQKGRIWLATTKGAQRYTDVKYSIDDTILFGPES
                     RGLPVELLAAHQEDCIRIPMLPDCRSLNLASSVAIVAYEALRQLGFPGLVP"
     gene            1657..>2089
                     /locus_tag="PLW82_07220"
     CDS             1657..>2089
                     /locus_tag="PLW82_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOJ47101.1"
                     /translation="MGLNITQKIIKEHLVSGEMIPGQEVGIKIDQTLTQDATGTMAYL
                     QFRAMDIPRVKTELSVSYVDHNTLQADFKNADDHIFLQTVAKKHGVYFSKPGNGICHQ
                     VHLERFGRPGKTLLGSDSHTPTGGGIGMLAIGAGGLDVAVAM"
BASE COUNT          527 a          514 c          475 g          573 t
ORIGIN      
        1 caaccttttc cttatcaagg atcaccgatt cggtaaaaac ttcttgaccg tgcagaattc
       61 tgtggcctac agcatctatt tcgtcaaggc tcttaatagc gccgagcttt ggatccatca
      121 aaagttttag cacttcctgc agtgcaacct tgtggtcggg aagttcgatg ttaatgacgg
      181 tcttttctcc gttttcgtgc ttaataaatg agccgtcaaa gccaattctc tcggcgatgc
      241 cgctcacgac agggactttt ttgtccatat caaagagttt gtacttcaaa gacgaactac
      301 cactgttgat aacaagaata ttcatcgatt actacgctcc tgttctttac ttatatctta
      361 aaaaaacatt actgttcttt attcttacac accagactta gtttaacatt taacaccgag
      421 tagcgtcaac aaaagtcctg gctaaatctg ggtaaggagg aagaccgctc ttctgttctg
      481 aaaactggag cgaaagctgt ttccagggac tcctagtttt ggacgtttgt ggaatgcagg
      541 ttgtggtata ataagctata tccagttggg aggaagtgat caccatcaag acgacgagaa
      601 atctgattac agcaagtctc cttgcagcta ctgccattgt gcttacgcgt tttttcggaa
      661 tccctcttgg tacggtcagg atttccctag gtacaatacc gatcatgtta tcaggtatcg
      721 tgctgggacc tttctggggt cttgcaacgg gaaccgttgc cgacctggtt ggtgtgctca
      781 tcaatcctca gggcggcgct tacttcccag gctttaccct taccaccgct ctggtcggat
      841 ttctgccggg gttcatcctg cggaacaaaa ggcctcctct gtggaagctt gctgtggcca
      901 taggcgttac cgaactcctc tgccacgtgt tcttaaatac cctctgggta ggcatgatta
      961 ccggtaaagc atacctagca cttctacctt ctagaatcat ctccagagtc gttcacttcc
     1021 cgattattac tggtgcaatc tacttcctaa ttcaaaaggt gttcgctccg aagaagacca
     1081 ccgaagacta gggcacaagt cccgggaagc caagctgcct caaggcttcg taagcgacga
     1141 tagccaccga acttgccagg ttcaaagatc tgcaatcagg gagcataggg atgcgaatgc
     1201 aatcctcttg atgcgctgca agcagctcca cgggaagtcc cctggattcc ggaccaaaca
     1261 gaatagtatc atcaatgctg tatttaacgt cagtatatct ttgggcgcct ttggtagttg
     1321 caagccaaat acgccctttt tgtttactct ggtagagttc ctctaaggtc gcagtccttt
     1381 taatggaatc tatgtctaag tagtccattc cggctctttt tacaagtttg tcgctgaggt
     1441 gaaaccctaa agcacctacc aggttcagtt ctaatccact tgcggtggca agcctggcaa
     1501 tgtttccagt gttttgaggg atttccggtt caaaaagggc tatttccaca tcgaccactc
     1561 ctttttcgca gttcctttac aagtatacac tgtgtctgct aggaatttta ggttttatca
     1621 cgtacaataa taagaacttt tgtatggagg tgcaacatgg gactgaacat tacccaaaag
     1681 atcatcaaag agcatcttgt ctcaggtgag atgatccccg gacaggaagt tggtataaag
     1741 atcgatcaaa cccttaccca agacgccaca ggtaccatgg cctacctgca gttcagagcc
     1801 atggatatac ccagagtaaa gacggagcta tctgtaagct acgtagacca caacacgctg
     1861 caggcggact tcaagaatgc cgacgatcat atctttctgc agaccgtagc taaaaagcac
     1921 ggggtgtact tcagcaagcc cggcaacggc atctgccatc aagtacacct agaaagattc
     1981 ggccgccctg gaaagaccct tttgggttct gacagccaca ccccgactgg cggcggtatc
     2041 ggtatgcttg ccatcggtgc tggaggtttg gatgtagcgg tagctatgg
//
LOCUS       DAOSDA010000317         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_35371_length_2081_cov_3.822804, whole genome shotgun sequence.
ACCESSION   DAOSDA010000317 DAOSDA010000000
VERSION     DAOSDA010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35371_length_2081_cov_3.822804"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLW82_07225"
     CDS             <1..379
                     /locus_tag="PLW82_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008184855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47102.1"
                     /translation="LFYKAFRRRSVYSTVKNPSQVVIALERGDSELFLQALDDEIHQP
                     FRGQLIPGYDALSAQAVKLGGRIVISGSGPTLLYLTAESPDTAKKAASDLSETWKEQF
                     AVENAVVQVKLGAKGARILEAED"
     gene            445..522
                     /locus_tag="PLW82_07230"
     tRNA            445..522
                     /locus_tag="PLW82_07230"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:480..482,aa:Pro,seq:ggg)
     gene            661..1518
                     /locus_tag="PLW82_07235"
     CDS             661..1518
                     /locus_tag="PLW82_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOJ47103.1"
                     /translation="MFPKKVLGLIVLFLVVLAGAAFAEEEVLTLKVMTYNIQIAATAN
                     NPGWVVANFDLDGIAEMIREQDPDIVALQEVDRYRGRSGYVDQVAELAEKLGMYWAYS
                     PSYFDYETANGRGMYGNAILSKYPIVNQYYHTLWRRATLEPGEASWVIEKRSLLETQI
                     DVNGTIVHAYSTHLSTTQDQRAEQTTEIMSLVAQTKGPKIIMGDFNSQPDAPEMKNLL
                     RRFRDVMEHAEVRSEQRNTYPNGTKADRVIDYILVSREWTIKEAQVIADEEGRSDHNP
                     IVAVLVLEK"
     gene            1637..>2081
                     /locus_tag="PLW82_07240"
     CDS             1637..>2081
                     /locus_tag="PLW82_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOJ47104.1"
                     /translation="MAQSKTNDRSVDFSAALQGYPRDPKYLLPVLYKLQENYGFLSTE
                     MLQEAARHLGVPVGKVYSLATFGEEFRLEPLGRCVIEVCSGACCRIKGSGGILSVLEA
                     ELGIKAGQTTQNGAFTLLEASCIDACALAPAMRVNGHLYGNLQGGD"
BASE COUNT          511 a          514 c          581 g          475 t
ORIGIN      
        1 cttattttac aaggctttca gaagacgctc tgtctattca actgtcaaaa acccgtctca
       61 ggtggtcata gccctcgagc gtggtgacag cgagctcttt ctgcaggctc tagacgatga
      121 gattcaccag cctttcagag gacagcttat accgggatac gacgccctga gcgctcaagc
      181 ggtgaaactc ggaggccgga tcgtcatttc gggctcgggc ccgacgctgc tctacctgac
      241 cgcagagtca cctgacacgg cgaaaaaggc agcctccgac ctgtcggaga cctggaaaga
      301 gcagttcgcc gtggaaaacg cagtagtaca ggtaaaactt ggtgcaaaag gagcaagaat
      361 cctggaagca gaggattaat tggcgctttc catcttgatt ttgccctgga atgtgctaca
      421 ataatccttg caggttagtt ctgtcgggct gtggcgcagc ttggctagcg cgcttccttg
      481 gggtggaaga ggtcgctggt tcaaatccag tcagcccgac catttttttg gagacaaccc
      541 agagaacgcc gtttgttagg cgtgtctctg ggtttttttg tgtcaccagc cggacttgca
      601 ggaaacctct tttcctttca agaaattaga aagcaaacca aataaagggg aggagtaagg
      661 gtgtttccta aaaaagtgct tggcttaatc gttttgtttt tagtggttct tgcaggagcg
      721 gcgtttgccg aggaagaggt tttgactctt aaagtgatga cctacaacat ccagatcgcc
      781 gcaacggcca acaaccctgg atgggtagtg gccaacttcg atctcgacgg catagcggag
      841 atgatcaggg agcaagatcc cgacatcgtt gccctccagg aagtggaccg ctaccgcggc
      901 aggagcggct atgtagacca ggttgcagag cttgcggaga aacttggcat gtattgggcg
      961 tactcgccgt cttacttcga ctatgaaacc gcaaacggca gaggtatgta cggcaatgcc
     1021 attttgtcga agtatcccat agtcaaccag tactaccata cgctgtggag gagagcgacc
     1081 ctagagccgg gagaagcttc ttgggtcatc gagaagagat ctctcctaga aactcagata
     1141 gatgtcaatg gaaccatcgt tcacgcctac tccacgcacc tttcgaccac tcaggatcaa
     1201 agagcggagc agaccacaga gatcatgagc ctggtagcgc agacaaaagg gcccaagatc
     1261 attatgggag atttcaactc ccaaccggat gctcccgaga tgaagaacct cctgaggcgg
     1321 ttcagggacg tcatggagca tgctgaggtt cgcagcgaac aacggaacac ctatccaaac
     1381 ggtactaaag ccgatcgggt tatcgactac atcttggttt cgcgggaatg gactattaaa
     1441 gaggcacagg tcattgccga cgaagaagga agatccgacc acaaccccat cgtggcggtt
     1501 ctggttttgg agaaataacc gggagggctg gtgccctcct tttttgtttg gcgatttccg
     1561 ctctttcttc ggttaacttg aagaaaggta attgccacaa ttttggcgaa cttctaccta
     1621 gactggaggt ggcaaagtgg cgcagtctaa gacaaacgat agaagtgttg atttttcggc
     1681 ggcactccag gggtatccaa gagaccccaa atacctgctg cctgtccttt acaagcttca
     1741 ggagaactac gggttcctca gtaccgaaat gctgcaggaa gcagctcgcc atctgggagt
     1801 tcccgtcggg aaggtttaca gccttgcaac gttcggagaa gagtttcgtc tcgaacctct
     1861 tggcagatgc gttatcgaag tctgcagtgg cgcctgctgc aggattaagg gttcgggggg
     1921 tatcctctcg gtgctcgaag ccgagctggg tatcaaagct gggcaaacta cgcaaaacgg
     1981 cgcgttcacg ctccttgagg ctagctgtat cgatgcctgc gcacttgctc ctgccatgcg
     2041 agtcaacggg catctctacg ggaacctgca gggcggagat g
//
LOCUS       DAOSDA010000318         2062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_35864_length_2062_cov_3.447434, whole genome shotgun sequence.
ACCESSION   DAOSDA010000318 DAOSDA010000000
VERSION     DAOSDA010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35864_length_2062_cov_3.447434"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLW82_07245"
     CDS             complement(<1..417)
                     /locus_tag="PLW82_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOJ47105.1"
                     /translation="MKLKDVLKPLKDFAPESTAEPWDNVGLQIGSQLDEVEKVLLTVD
                     VTESTVDEAVEKGCQLIVSHHPMIFKPLKSLEVAGLVPRTLVKAIKNNIAIYCAHTNL
                     DLAEGGVNHALSDALGLLGGRVKGLVPSGYNEFAKVV"
     gene            complement(423..1088)
                     /locus_tag="PLW82_07250"
     CDS             complement(423..1088)
                     /locus_tag="PLW82_07250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ47106.1"
                     /translation="MIPERLKRLAELVGRIGTGADVGCDHAYLSHYLLKNDLAQYMIA
                     TDINMNPLKYARRTLRPFQSRSQIRQGYGLRPIDPGEVDAIFIGGLGAETIVEILEDG
                     MERFPGTTFALQVNGDPSPLREFLLSKPIYREDIIKENRHYYQIIIVKEGYSHGEQIW
                     NGTGPILQIGTLYKTEVGKNYLKKCLADIEEALRGIGPADVRGQHLREKHNFFTQLLS
                     AEE"
     gene            1159..1749
                     /locus_tag="PLW82_07255"
     CDS             1159..1749
                     /locus_tag="PLW82_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47107.1"
                     /translation="MKLNRLALVIIILTALTFSTSAAEYNLIAGEPLGLFNNTFSGYY
                     SKISVRNAHVVAFPLSYSEAVNVKYLSAGVEYRKYLSGNLAGLYFETNLAASLQDKAY
                     DPDILTLTPAFVVGYRIDAGQGLFVEPELTYTLPMVSVGGTALEVAKDFESSFNIVLG
                     LDNVYPNGIFFAPRLSFSYSGGTLHAVGKLFLGYGF"
     gene            1774..1959
                     /locus_tag="PLW82_07260"
     CDS             1774..1959
                     /locus_tag="PLW82_07260"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HOJ47108.1"
                     /translation="MPICTIKLLEGRTDEQKKALVEKVTQAIHETTGAGPEHITVILE
                     EMQRNHYAVGGVRASDK"
BASE COUNT          504 a          544 c          466 g          548 t
ORIGIN      
        1 caccactttg gcaaactcgt tgtaccctga aggcacaagg cccttcaccc tacctcccaa
       61 aaggcctaaa gcatcagata gcgcgtgatt caccccacct tctgcaaggt ccaagttcgt
      121 gtgggcgcag tagatagcta tattgttctt gatggcttta acaagggttc tcggcacaag
      181 accggctact tctaggctct ttagaggttt gaaaatcata ggatgatggc tgacaataag
      241 ctggcagccc ttctccaccg cttcatcaac ggtactttct gtgacgtcta ctgtaaggag
      301 caccttttcc acttcgtcta attggcttcc aatctgcaat ccaacgttat cccaaggctc
      361 cgcggtgctt tccggagcaa aatccttcag cggtttgagt acgtctttga gcttcacctc
      421 gatcactcct ctgcacttag tagttgcgta aagaagttgt gcttctccct cagatgctgc
      481 ccacgaacgt ctgcaggacc aatgccccgc aaggcttctt caatatcagc aaggcacttc
      541 ttcaggtagt ttttgccaac ctcagttttg taaagggttc ctatctgtaa aataggaccg
      601 gtcccattcc agatttgctc tccatgactg taaccttctt taacgatgat aatctgataa
      661 taatgccgat tctccttgat gatatcttct ctgtagattg gcttggacaa aagaaattcg
      721 cgaaggggtg aaggatcacc gttaacttga agagcaaacg ttgtacctgg aaatcgttcc
      781 attccatcct ctagtatttc cacaatggtc tcggcaccca gaccgccaat gaagatagca
      841 tcaacctctc ccggatcaat aggcctgaga ccgtatccct gccggatctg agagcggctc
      901 tgaaaaggcc tgagggtcct tctggcatac tttaacggat tcatgttgat atcggtggcg
      961 atcatgtact gggccaaatc gtttttcagg aggtagtggc ttaagtaagc gtggtcacac
     1021 cccacatcgg caccggtacc gatcctgcct acaagctctg ccagtctttt taatctttcc
     1081 ggtatcattt cgtagtctcc ttcaaaccaa gtgcgcaagc tgcaaagcat atcatctaca
     1141 cgaaaggagc caattacaat gaagcttaac agactcgctt tggtaatcat tatccttact
     1201 gccttaacct tttcgacttc tgcagcggag tacaacctca tagctggaga acctctaggc
     1261 ctcttcaaca acactttctc tggatattat agcaaaatat ctgttagaaa tgctcatgtt
     1321 gttgcgtttc cgctgagcta cagcgaggca gtcaacgtga agtacctgtc ggcaggagtg
     1381 gaataccgca agtacctatc gggcaatctt gcaggtctct acttcgagac aaacctcgcc
     1441 gcgtcgcttc aagacaaggc ctatgatccc gatatcctca cccttactcc cgcttttgtt
     1501 gtaggataca gaatcgacgc cggacagggt ctgttcgtag agccggagct gacctacacg
     1561 ctgcctatgg tctctgtagg cggcacagcg ctggaggtgg cgaaggactt tgagagttcg
     1621 ttcaacatcg tccttggcct ggataacgta taccccaatg gcatcttttt cgcacctaga
     1681 ctgagctttt cctactcagg tggcacccta catgcagtag ggaagctgtt cctcggttac
     1741 ggattctaaa tctaaagtta ggaggtacta tccatgccaa tttgcaccat caaactgctt
     1801 gaaggacgca ccgacgagca gaagaaagct ctggtagaaa aggtaactca agcgattcac
     1861 gagacaactg gagcaggtcc cgagcacatc accgtgattc tcgaagaaat gcagcgcaac
     1921 cactacgccg taggcggtgt acgggcatct gacaagtaag tctgaagcag gtttccctgc
     1981 ttcttttttt tatacctttc atgcccacgg tttcagagtg gaagcccttt tcccgggtca
     2041 ggagtttggt ttcctcaggg ga
//
LOCUS       DAOSDA010000319         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_36145_length_2050_cov_2.714286, whole genome shotgun sequence.
ACCESSION   DAOSDA010000319 DAOSDA010000000
VERSION     DAOSDA010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36145_length_2050_cov_2.714286"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PLW82_07265"
     CDS             complement(<1..703)
                     /locus_tag="PLW82_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOJ47109.1"
                     /translation="MLDSTIRKVLVVGSGPVVIGQAAEFDYAGTQACRALREEGVEVI
                     LLNSNPATIMTDKEMADRVYLEPITVELCEQIIRKERPDGFIAGLGGQTGLNMALALA
                     QSGVLKKYGVKLLGTRLDAIKQAEDRELFKKLIEEIGEPVCPSSTVTSVERALAVAEE
                     IGYPVIVRPAFTLGGTGGGMAKDESELKRLAGLGLEASPIGQVLIEKSVAGYKEIEFE
                     VIRDRAGNAISICSME"
     gene            complement(697..1635)
                     /locus_tag="PLW82_07270"
     CDS             complement(697..1635)
                     /locus_tag="PLW82_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HOJ47110.1"
                     /translation="MKRSSGDKRPVGLYLDDGTFFAGKWGFGSVSGSVVVVDSSGFVE
                     AATDSAADGLILCFTFPQAGFAPVALREVESQGSRASGVIISQIARTKRGEMLKEYFR
                     KQEIALLEGVDTRALALHLSAKPVSGVIYTGVEIQGTAGEPLTRTTPNRGARVAVLGE
                     LTHSLGRNLLDLGCELIEYEDVPAEFFQIDAVVLTKALENDQLQALASGSVPVIALGR
                     GAHSFAVSFGGQLRKSPLFAKNLAVEDLRSSEKRILSAEFRLKETVVKAPGKVVFLKH
                     LSRRPVGFAVGSAICLEFLPDKELLKELLERRWFSC"
     gene            complement(1625..>2050)
                     /locus_tag="PLW82_07275"
     CDS             complement(1625..>2050)
                     /locus_tag="PLW82_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ47111.1"
                     /translation="NPPIREPEDRDALKKALLDGTIDAVATDHAPHTEDEKARPLDEA
                     PFGISSIEVAASIVYTCFQDTTVLYEKLCKKPYEIIGEKNELEVGDPSDLTVFNPRAN
                     FVVSADSWASKGKNSPYFGRTLKGRVTLTIQGGRIVYEA"
BASE COUNT          433 a          613 c          459 g          545 t
ORIGIN      
        1 tttccatgct gcagatgctg atggcatttc ctgccctgtc ccggatcacc tcaaactcga
       61 tctctttgta ccctgccaca gacttctcga tcagaacctg cccgatgggg cttgcctcaa
      121 gtcccagtcc tgcaagtcgc ttcaactcac tctcgtcttt ggccattccg cctcccgttc
      181 cccctaaggt aaaggccggc ctgacaatga ctgggtagcc gatctcctcc gccaccgcta
      241 gagcccgctc aacagacgtg acggtgctgc tgggacagac aggctcgcct atttcctcta
      301 taagcttttt gaagagttct cggtcttctg cctgctttat agcatcgagc cttgtaccaa
      361 gcagttttac cccgtacttc ttcaagactc cggactgggc aagtgccaaa gccatgttca
      421 gtccggtctg tccacccaga cctgcaataa acccgtctgg tctttctttc cggatgatct
      481 gctcgcacag ctctacggtt attggctcaa ggtagactct gtctgccatt tccttatcgg
      541 tcatgatcgt tgcaggattg gagttgagca gaattacctc gactccctcc tcacgtagtg
      601 cccggcacgc ttgggttccg gcatagtcga actctgccgc ctgcccgata acgacaggcc
      661 cggaccccac aaccaggact ttcctaatag tgctgtctag catgagaacc accttctttc
      721 caggagctcc ttgagcagct ctttatccgg gaggaactcc aaacagattg cactcccaac
      781 tgcaaaaccg acgggtcttc gggataggtg tttcaaaaaa actacctttc cgggagcttt
      841 tacaactgtt tctttaagac gaaattccgc tgacagaata cgcttttcgc tactcctcaa
      901 gtcttcgaca gctagattct tggcaaaaag ggggcttttg cgcagctgcc ctccgaaact
      961 caccgcaaag ctgtgggcac ctctacctaa ggcaatcacg gggacgcttc cggaagctaa
     1021 agcctgcagc tgatcgtttt caagggcctt tgtcagaaca actgcgtcga tctggaaaaa
     1081 ctccgcaggt acatcctcat actcaatcag ttcgcagccc aaatccagga ggttcctccc
     1141 aaggctgtgg gtaagttctc ccaaaactgc aactctagct cccctgttgg gagttgttct
     1201 ggttagcggt tctcctgcag ttccttgtat ttcgactccc gtgtagatca cccctgagac
     1261 aggtttggcg cttaaatgca gcgctaaggc tcttgtgtcc actccctcaa gtagagcgat
     1321 ttcctgcttg cgaaaatact ctttcagcat ctcgcccctt ttggttcttg caatttgcga
     1381 gatgatcacc ccgcttgccc tacttccttg ggattccacc tctctcaagg caactggtgc
     1441 aaacccagcc tgaggaaagg taaaacatag tattagtccg tcggcggcac tatcggtggc
     1501 cgcttctaca aatcccgatg aatctacaac cacaacagaa ccactgacgc tgccaaatcc
     1561 ccactttcct gcaaaaaagg taccgtcatc caaataaagg cctaccggcc ttttgtctcc
     1621 actactacgc ttcatagacg attctgcctc cctggatcgt aagggttact ctacctttta
     1681 gggtcctgcc aaagtacgga ctgttcttcc ctttggaagc ccagctatct gcgcttacca
     1741 cgaagtttgc ccggggattg aaaaccgtaa ggtctgatgg atcccctacc tccagctcgt
     1801 tcttctctcc gataatttcg taaggctttt tgcacagctt ctcgtacaag accgttgtgt
     1861 cttggaagca ggtgtatacg atacttgcag cgacttctat gctgctgatg ccaaacggag
     1921 cttcgtctag aggtctagct ttttcgtctt cggtatgggg agcgtggtct gttgccactg
     1981 cgtcgatcgt cccatcgaga agcgccttct tcagtgcgtc tcggtcctcc ggctctctaa
     2041 tgggagggtt
//
LOCUS       DAOSDA010000320         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_36329_length_2042_cov_3.862607, whole genome shotgun sequence.
ACCESSION   DAOSDA010000320 DAOSDA010000000
VERSION     DAOSDA010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36329_length_2042_cov_3.862607"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLW82_07280"
     CDS             complement(<1..382)
                     /locus_tag="PLW82_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HOJ47112.1"
                     /translation="MSKALRMRLGLIFFLVIVMYGLVVLKLFKLQVVDAAELSERALY
                     QRLRIDTITPERGDILDRNGKILAESIPTPAVYADPSVIKDPKTTAEKLAPVLEMSEE
                     RLYELLTRPGSFVWLTRDLPIGKRS"
     gene            complement(379..828)
                     /locus_tag="PLW82_07285"
     CDS             complement(379..828)
                     /locus_tag="PLW82_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47113.1"
                     /translation="MTYQEVKKQHTVKTKTRVEENFPAIVILVLIIAIPFVLVAVLQG
                     QVSSLSLKLTEANQLLQVENEKLKQLNYQLKELQSLQRVEEVALSLGLVNNTDTEIVV
                     LVPPSARPASTERIDAGPATVAGAVPASLFDRLLDFFYGLLGGSELL"
     gene            complement(825..1796)
                     /gene="rsmH"
                     /locus_tag="PLW82_07290"
     CDS             complement(825..1796)
                     /gene="rsmH"
                     /locus_tag="PLW82_07290"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOJ47114.1"
                     /translation="MKTVEHLPVLFHESVEMMKPGPGKTIVDCTLGRGGHTKGFLDRG
                     ASVIALDQDLEAIKKVAENFNLHKVFDNPLVYKGDQLTVVHANFKQLSSVLYRLSVFE
                     VDGVFWDLGVSSPQFDEPERGFSYRFDAKLDMRMDQSQSITAWDVVNTYDLDELTRII
                     REYGEERYAKRIADQIAIARPINTTFELVEVIKKAMPKRALSEQHPAKRTFQAIRIAV
                     NDELGALTTSLEKGLEHLKLGGRLVVISFHSLEDRIVKDFFKEKEDPCTCPKRLPVCV
                     CGKKPIVKVITKKPVVPDALEVEENRRAHSAKLRVAEKIYSDKEVGK"
     gene            complement(1799..>2042)
                     /locus_tag="PLW82_07295"
     CDS             complement(1799..>2042)
                     /locus_tag="PLW82_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOJ47115.1"
                     /translation="DVRAFTRFFLSAAAEAELDKQGRINLPSNLKLHAGIEKEAMVVG
                     VSNRIEIWEPAAWQKYMDSFSSSITEIAENLVDLGI"
BASE COUNT          485 a          542 c          444 g          571 t
ORIGIN      
        1 tcgaacgctt gccgatggga agatctctcg taagccacac aaagcttccg ggtcttgtga
       61 ggagttcata aagccgctct tcactcatct ccaaaactgg agccagtttt tctgcagttg
      121 tcttgggatc cttgatcacg ctaggatctg cgtagactgc aggagtagga atactttctg
      181 caaggatttt gccgtttcga tccagaatat cgcctctttc aggcgtaatg gtgtcgatgc
      241 gtaacctttg atacagcgct ctttccgaaa gctcagcagc atcgaccacc tgcagcttaa
      301 acagcttcaa aaccacaagt ccatacatca ctattaccaa aaaaaagatg aggccaagcc
      361 tcatcctcaa tgctttgctc ataacagttc agaccctccc aatagaccgt agaaaaagtc
      421 tagcagcctg tcgaaaaggc ttgctggaac ggctcctgct acagtggcag gtcccgcatc
      481 gattctttct gtagaggcag gtcttgcact tggaggtact aaaacgacga tttctgtgtc
      541 ggtgttgtta actaaaccta aagacaatgc tacttcttcc actctttgta gggattgaag
      601 ctctttcaac tggtaattca attgtttgag tttttcattt tccacctgta agagctggtt
      661 tgcttcggtt agtttcaatg aaagactcga cacttgtccc tgaagtacgg ccacaagcac
      721 aaacggtatg gcgataatca gaaccaggat cacaatagca ggaaagttct cttccactct
      781 cgtcttcgtc ttcacagtat gttgtttttt aacttcttgg taggtcactt tcccacctcc
      841 ttgtctgagt agatcttttc tgccacccgc agttttgcac tgtgagccct tctgttttcc
      901 tccacttcaa gagcatcagg gacaaccggt tttttcgtga tgacctttac tatgggcttc
      961 tttccgcaga cacaaaccgg aagtctcttc ggacaggtac acgggtcttc cttttcctta
     1021 aagaagtcct tcacgatccg atcctccaga ctgtggaagg aaatcacgac cagccttcct
     1081 ccgagtttca ggtgctccaa gcccttttcc aaactagttg tgagagcacc gagctcgtca
     1141 tttaccgcaa ttctaatagc ttgaaaagtt ctttttgctg gatgttgctc ggagagtgcc
     1201 ctctttggca tggctttctt gatgacttcc accagttcaa aagtagtgtt gatcggtctt
     1261 gcaatggcta tctgatcggc gatacgcttg gcgtagcgct cttctccgta ctctcttatg
     1321 attctggtca gttcatccaa gtcgtaggtg ttgaccacgt cccaggcggt gatgctctgg
     1381 ctctgatcca tcctcatgtc cagtttggca tcgaagcggt aggaaaagcc cctttccggt
     1441 tcatcgaact gaggggacga aacccccagg tcccaaaaga caccgtccac ctcgaacact
     1501 gacagcctat acagaacgct cgacagctgc ttaaagttgg cgtggacaac ggtcagctgg
     1561 tcgcctttgt aaaccagagg attgtcgaat accttatgca aattaaagtt ttccgcaact
     1621 ttttttatcg cttccaaatc ttgatcgagg gcaattacgc ttgctccccg atccaaaaaa
     1681 cccttggtgt ggccacccct tccgagggta cagtccacta tggttttccc ggggcccggc
     1741 ttcatcatct caaccgactc gtgaaacaga acaggtaagt gctccacggt tttcacccct
     1801 aaattccaag atcaaccagg ttctctgcga tctcggtaat agaactgctg aacgaatcca
     1861 tgtacttctg ccaggccgct ggctcccaaa tctcgatgcg gttcgataca ccaaccacca
     1921 tggcctcttt ttcaataccg gcgtggagtt tgaggttgga aggaaggtta attcttccct
     1981 gtttgtccag ttcagcttcg gctgctgcag acaaaaagaa cctggtaaag gccctaacat
     2041 ca
//
LOCUS       DAOSDA010000321         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_36404_length_2039_cov_2.990423, whole genome shotgun sequence.
ACCESSION   DAOSDA010000321 DAOSDA010000000
VERSION     DAOSDA010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36404_length_2039_cov_2.990423"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PLW82_07300"
     CDS             <1..833
                     /locus_tag="PLW82_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOJ47116.1"
                     /translation="EQISKETKIRLDYLEAIESGEFHRLPGEFYSRTYIRAYAKTIGL
                     DEDVVMEKYRSLLETAEAEGSEEGRPVVKKWDGTLQSTVASKGKAPRERKGRRVFWFL
                     LIIAILALGAVLLLDYLGYFDFEEDYQAPDIPEATVESTDEAVDLTTPDIDDLATDAT
                     LPDQNPADIGEAEPQEETEQVPETQEESLPKTFTLVLKASGDCWYEIRTESGTTIRSG
                     LLRSGQEVSTAGTQALRVLLGKPEVITLVIDDSTVAIPDDVRELIVDSSGQVNILNRW
                     "
     gene            838..1701
                     /gene="hslO"
                     /locus_tag="PLW82_07305"
     CDS             838..1701
                     /gene="hslO"
                     /locus_tag="PLW82_07305"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOJ47117.1"
                     /translation="MDHLVIGLAYGKFRIAAITGKDTVEEARKRHNLSHTATAALGRS
                     LLGAGLLASSFKSDKERILLQFRGDGPVSPIVAMADRNVNVRGYCENPQVELELNSQG
                     KLDVGGAIGSGMLYVIRERDNYEPYTGTVPIQTGEVGDDLAYYLQISEQVPSAVGLGV
                     LVDPTGEVRSAAGLLVQVLPGVPEEDIIQLEEKLMNLGSLSHRFDQGETPESLLDDLF
                     EGWNKLEERPLKFACDCSKERFQRGLFSLGREELSDLLETQQSVETVCRFCGEKYEFS
                     REEIEELMKKA"
     gene            1713..>2039
                     /locus_tag="PLW82_07310"
     CDS             1713..>2039
                     /locus_tag="PLW82_07310"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOJ47118.1"
                     /translation="MRRLWLWVLGVFLVFAVFFVLGADFLADVLWHDSMGYVDSFYKI
                     LGLKILPGLVAFVLIYLFLMINWLFVRKTWEAEREEEGLEPVKVSKWLTGVLGVLLSF
                     AFTSNIL"
BASE COUNT          521 a          452 c          585 g          481 t
ORIGIN      
        1 tggagcagat ttctaaagaa acaaagatcc gactggacta cctggaagca attgaaagcg
       61 gagagtttca cagactcccc ggtgagtttt actctcgcac ctatatcaga gcttatgcca
      121 agactatcgg gctagacgaa gatgttgtga tggagaagta ccgcagtctc ttggagacgg
      181 cagaggccga gggtagcgaa gaggggagac ccgttgtcaa gaagtgggat ggtacgctgc
      241 agagcacagt agcgtcaaaa ggtaaggctc ccagggaacg caaaggcaga agagttttct
      301 ggttcctttt gatcattgcc atacttgctt taggtgcagt attactcctc gactatttgg
      361 ggtattttga ctttgaagag gattatcaag caccggacat tccggaggca actgttgaaa
      421 gcacagatga agcggttgac ttgacaacgc ccgacatcga tgacctagcc accgatgcaa
      481 cgctgcccga ccagaaccca gctgacatcg gagaggcaga gccccaagaa gaaacagaac
      541 aggttccaga gactcaggaa gaaagccttc cgaagacttt tactttggtt cttaaagcca
      601 gcggtgactg ctggtatgag atccgcacag aaagcggaac gacgatccgc agtgggcttt
      661 tacggtctgg gcaggaggtt tccactgctg gaactcaagc cttgagagtc cttttaggca
      721 agcccgaagt aatcactttg gtgatcgatg atagcacagt agctataccc gatgacgtcc
      781 gcgagttaat tgtggacagc tcggggcaag taaatatttt gaatagatgg tgaaacaatg
      841 gatcatttgg taataggatt agcatacgga aagtttcgca ttgcagcgat cacaggtaaa
      901 gacacagtag aagaagctag aaaaagacac aatctctcgc acactgctac agcggcacta
      961 ggacggtcgc tccttggtgc cggtcttctt gcaagttctt ttaaatcgga caaagaaaga
     1021 atactcctgc agtttcgcgg cgacggaccg gtgtcaccga ttgtggctat ggccgatcgc
     1081 aacgtgaacg tcagggggta ctgcgagaat cctcaggtcg agctggaact aaactcccag
     1141 ggcaagctcg atgtaggtgg agcaatcggc agcggtatgc tgtacgtgat cagagagcgc
     1201 gacaattacg agccctacac cgggactgtg cctattcaaa ccggagaggt cggagacgat
     1261 ctggcctatt acctgcagat ctcggagcag gtcccatcag cggtgggtct tggtgttttg
     1321 gtagatccca ccggggaagt gcgctctgca gcaggccttc tggttcaggt ccttcccgga
     1381 gtgcctgagg aagacatcat tcagcttgag gagaagctga tgaatctcgg ctctttgagc
     1441 caccggtttg atcagggaga aaccccagag agccttctgg acgacctttt tgagggatgg
     1501 aacaaactgg aagagcgtcc gctcaagttt gcctgcgact gcagcaagga acgcttccaa
     1561 aggggccttt tcagtttggg ccgtgaagaa ctatccgatc tcttggagac gcagcagagc
     1621 gtggaaacag tatgccggtt ctgcggtgag aagtacgagt tcagcagaga ggaaatcgag
     1681 gaactgatga aaaaagccta gggagtgttg atatgcgcag actttggctc tgggttctag
     1741 gtgtttttct ggtttttgca gtgttttttg tcttaggcgc cgattttctt gcagacgtcc
     1801 tttggcatga cagtatggga tacgtcgatt ccttctacaa gatccttggt ctcaaaatct
     1861 tgccggggct agtagcattt gtcctaatct atctgtttct aatgatcaac tggttgtttg
     1921 tccggaaaac ttgggaggct gaaagggaag aggaaggttt ggagccggtg aaggtaagca
     1981 aatggctgac cggcgtcttg ggcgtccttt tgtcgtttgc ctttaccagc aacatcctc
//
LOCUS       DAOSDA010000322         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_36609_length_2032_cov_3.845726, whole genome shotgun sequence.
ACCESSION   DAOSDA010000322 DAOSDA010000000
VERSION     DAOSDA010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36609_length_2032_cov_3.845726"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLW82_07315"
     CDS             <1..402
                     /locus_tag="PLW82_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47119.1"
                     /translation="EGTIRALSQESRKKIGESLKRHAEYTAKAFGLEAEVEYVEQYPP
                     TANHQTALEIVQANLPQGLTYKELSFPSMAAEDFSFYTEKVTGAMLFLGSRTEECSMP
                     LHNSKFKFNEKIIGYGAELFLSVGRSLLKSR"
     misc_binding    474..716
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            748..1743
                     /gene="aroF"
                     /locus_tag="PLW82_07320"
     CDS             748..1743
                     /gene="aroF"
                     /locus_tag="PLW82_07320"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOJ47120.1"
                     /translation="MIIVMKPEASSVEIKEVCSKLETLSFSCHVIHGVNQSVIGVVGT
                     ITDELLEQAQLWPKVEKAIRVGKPYKLVSRECQPEGSVVKVGSVSIGKEKVVIAGPCA
                     VETEDQMLKTAEAVKKAGANMLRGGAFKPRTSPYAFQGLKTKGLELLSEAGRAYGLPV
                     VTEVVDPRDVEIVAQYADMLQIGARNMQNFSLLKEVGLTRKPILLKRGLSSTIQEWLM
                     AAEYILAEGNSQVVLCERGIRTFEPWTRNTLDISAIALAKETSHLPVLADPSHASGKR
                     SLVEPLAKAALAAGADGLIIEVHPNPDGALSDGPQSLNFEEFERLMARLNLGEGV"
     gene            1740..>2032
                     /locus_tag="PLW82_07325"
     CDS             1740..>2032
                     /locus_tag="PLW82_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOJ47121.1"
                     /translation="MIYYLGPEGSYSHEAALLLDEDAIAVKSIKEVFQNSRNGRGIVP
                     WENSIEGSVGETLDLLLESEVHVVGEVNLQISHCLLGYENETNRIVYSHPQAIG"
BASE COUNT          545 a          470 c          577 g          440 t
ORIGIN      
        1 gaagggacca ttcgggctct gtcgcaggag tcaagaaaga agattggcga gagcctgaag
       61 aggcacgccg agtatacagc taaagccttt gggttggaag cggaggtgga gtatgtcgaa
      121 cagtatccgc cgaccgcaaa tcaccagacg gctctggaaa ttgtacaggc aaacctccca
      181 caaggcttaa cttataagga gttaagcttt ccctcgatgg ctgctgaaga cttctccttt
      241 tacaccgaaa aggtcacagg tgccatgctc tttctcggaa gcagaacaga agagtgcagc
      301 atgcctctgc acaacagcaa gttcaagttc aacgaaaaaa tcatcggtta tggcgccgag
      361 ctcttcttaa gtgtaggcag gtcgctcctt aagagccggt aatgaaatgg cgaaaaaagc
      421 gactttttct tcttgacaag gcgtctggtg cttgctaata tgttttcaat atattaaaga
      481 ctgtgaaggg gaaaagtacc ttgcggggga cttatagaga gccggggatg gtggaaaccc
      541 ggtagaaagc cgtaagcgaa gttctccccg gagttgcccg gttgaacgca aagtagatcg
      601 ggacggaaac ttccaccgtt aaaaggaagc gggtatcagc gctaagctcg tacctgatag
      661 gagtgggctg gaaacagcca attagggtgg taacgcgaga aaacctcgtc cctttggggc
      721 gaggttattt ttttaggagg gaaagctatg atcatcgtta tgaagccaga agcctcgtcg
      781 gtggagatta aggaagtctg cagtaagctc gagactttga gtttttcctg tcacgtgatc
      841 catggagtta atcaaagcgt aatcggcgtc gtcggcacca tcaccgatga actccttgag
      901 caggcccagc tctggccgaa ggtggaaaag gctattagag ttggtaaacc ttataaactt
      961 gtcagcaggg aatgccagcc tgaaggcagt gtggtcaaag taggaagcgt ttccatcggc
     1021 aaggaaaaag tagtaattgc cggtccatgt gcggtggaga cagaggacca gatgctaaaa
     1081 accgcagaag cagtgaaaaa agccggagcc aacatgctcc ggggcggtgc tttcaagccc
     1141 cgcacatcgc cctacgcctt ccaggggttg aagacaaaag gacttgagct cctcagcgaa
     1201 gcaggaagag cgtacggtct tcctgtagtt acagaggtgg tcgatcctcg ggacgtggaa
     1261 atcgtggcac agtatgcaga catgctccaa atcggtgcgc gtaacatgca gaacttctct
     1321 cttttgaagg aagtcggcct gaccagaaaa cccatcctct tgaagcgcgg actttcatct
     1381 acaattcaag agtggctgat ggccgcagag tatattcttg cggaaggaaa cagccaggtg
     1441 gtgctgtgcg aaagaggtat cagaaccttc gagccctgga ccagaaacac tctggacatc
     1501 tctgcaattg cgctagcaaa agaaaccagc cacctgccgg ttttggcaga tcccagccat
     1561 gcatcgggca agcgaagcct tgtggaacct ctagcaaagg ctgcccttgc agctggagca
     1621 gacggcctga tcattgaggt ccatcccaat cccgacggag ctctctctga cggaccccag
     1681 tccctgaact tcgaggagtt tgaacgtctg atggcccgtt taaatctagg tgagggcgta
     1741 tgatctacta cttgggaccg gaaggttcct actctcacga agcagcgctg cttctagatg
     1801 aagatgcaat tgcggtgaag tccattaaag aagtcttcca aaacagcagg aatgggcggg
     1861 ggatcgtacc gtgggaaaac tccatagagg ggtctgtagg tgagactctg gatcttctgc
     1921 tcgagtctga agttcatgtg gtgggagagg tgaatctgca gatctcccac tgcctgttgg
     1981 gctatgagaa cgagacgaac agaattgtct actcccatcc ccaggctata gg
//
LOCUS       DAOSDA010000323         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_36619_length_2032_cov_2.979262, whole genome shotgun sequence.
ACCESSION   DAOSDA010000323 DAOSDA010000000
VERSION     DAOSDA010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36619_length_2032_cov_2.979262"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..1500)
                     /locus_tag="PLW82_07330"
     CDS             complement(313..1500)
                     /locus_tag="PLW82_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOJ47122.1"
                     /translation="MYEFLLKRFVKNYKDVHRPKVREDYGRLSSIVGAASNVLLAALK
                     FAVGFATRSVAIMGDAVNNLSDAGSSLITVISFKLAGKPADEEHPFGHARIEYIASSL
                     VAVLILVIAVELFMESVSRIINPVEMHFGLAAYVVLTFSILVKLWLSTFNRRLAGKIG
                     SPILEATAADSLSDVLATSAILASNIISPLVGISLDGYMGVGVSVFITISGVSILKET
                     LNRIVGQKPSDEMVEKIDSYIKKHEGIIDTHDLVVHEYGPGRCFATVHAEVNANGDFL
                     ESHDLIDNIERNISVDLGIHLVIHIDPVITDDPYVNELKEKTERVVVSIDPELSMHDF
                     RVVKGKTHCNLVFDVTVPAGYKKSDKQLIREISEKISELDKSFYAVVTVDRAYTSLTN
                     LLK"
     gene            complement(1540..>2032)
                     /locus_tag="PLW82_07335"
     CDS             complement(1540..>2032)
                     /locus_tag="PLW82_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOJ47123.1"
                     /translation="ETGGRRIKRSVFIDMTSIRFLTKEDIERLKKIHYLKDYIEAKEK
                     EIEEYNKKHNINPEIKVNGRHLTNIGTFRAYITNYLKNHPQLNPTLLQIVRQLDPGPN
                     GLPLEIYCFTADTRWVVYEGVQSDIFDHILAVASEFDLRIFQNPSGNDLRELAGGAVF
                     RER"
BASE COUNT          497 a          496 c          479 g          560 t
ORIGIN      
        1 gactgcagca gatagattgt ttattttcta tcaacctaaa tctaaaataa agccagcact
       61 tatcagcgtt gggctaataa atgttggctg aaagtccatt tattaccgct gtgttcctta
      121 atacctttat gaagctgtaa gctgcgcatc ccaaaacaaa aaaatagaac ccgagaacct
      181 ctacgcagtc catgggagtt tttgccgctt catccctttt tgcaaactct cctaatgtat
      241 atgatataac taattctgca agttgtcacc tgtccggagc agtgtattca gtattatttg
      301 cccaagatgg ggttacttca gtagattggt cagcgaggtg taagcccgat ctacggtgac
      361 taccgcatag aagctcttgt ccagctcgga gatcttttcg gagatctctc tgatcagctg
      421 cttgtctgat ttcttgtacc ctgcgggaac tgtaacgtca aagaccaggt tgcagtgtgt
      481 cttacctttg accacccgaa aatcgtgcat actcaattcc ggatcgatgg aaaccacaac
      541 cctttctgtc ttttccttca gctcgttgac atacgggtcg tcggtgatca caggatcgat
      601 gtgtatcacc aggtgaatgc cgagatctac ggagatgttc ctctcgatat tgtcgatgag
      661 gtcgtggctc tcgagaaagt cgccgtttgc attaacttca gcatgcaccg tagcaaagca
      721 gcggcctggt ccgtactcgt ggactaccag gtcgtgggtg tcgatgatgc cttcgtgctt
      781 cttgatgtag gagtctatct tctccaccat ctcgtcggag ggtttttgcc ccacgattcg
      841 gttgagggtt tctttaagta tggatactcc gctgatggtg atgaaaacgg aaacccctac
      901 tcccatgtaa ccgtcgagag agatgcccac caaaggagaa atgatattgg aggccaggat
      961 ggcagatgtg gcaagaacgt ccgacaagct gtctgctgca gtagcctcga gtatgggcga
     1021 gccgatcttt cctgcaagcc gtctgttgaa cgtagaaagc caaagcttca ctaaaatgga
     1081 aaaggtcaga accacatatg cagccaggcc aaagtgcatc tctacaggat tgatgattct
     1141 cgagacagac tccataaaca gctctacggc gataactaaa atcaatactg caacaaggga
     1201 agatgcgatg tactcaatgc gtgcatggcc gaaaggatgc tcctcgtctg caggttttcc
     1261 tgccagtttg aagctgatca cggtaatcag cgatgagcct gcatccgaca ggttgtttac
     1321 agcatcgccc ataatggcaa cactgcgtgt ggcaaaccct acagcaaact tcagcgcagc
     1381 cagcaaaacg ttgctagctg cgcccactat gctgctcagc cggccgtagt cttcccttac
     1441 cttaggacgg tgcacgtctt tatagttttt tacaaatctt ttcagcaaga actcgtacat
     1501 tactctctgc tcctacaacc ggggtgacac ccggtttttt taacgctcgc ggaaaacagc
     1561 tccgcctgca agctctctga ggtcgtttcc cgaaggattc tggaagatcc tcaggtcgaa
     1621 ctcgctggca acagctaaga tgtggtcgaa gatatccgat tgcactcctt catagactac
     1681 ccatctggta tcggcggtaa aacaatagat ctccaaaggc agtccgtttg gtccgggatc
     1741 aagctgtctg acaatctgca ggagcgttgg atttaactgg ggatggttct tcaagtagtt
     1801 agtgatgtaa gccctaaagg tgccgatatt tgtcaagtgc cttccgttga ccttgatttc
     1861 gggattgatg ttgtgctttt tattgtactc ttcgatctct ttttccttgg cctcgatgta
     1921 atccttgagg tagtgaatct ttttcaagcg ttcgatatcc tctttcgtga ggaacctgat
     1981 ggaggtcatg tcgatgaaaa ccgaacgctt gatccttcgc cctccagtct ca
//
LOCUS       DAOSDA010000324         2019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_36940_length_2019_cov_3.528004, whole genome shotgun sequence.
ACCESSION   DAOSDA010000324 DAOSDA010000000
VERSION     DAOSDA010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36940_length_2019_cov_3.528004"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLW82_07340"
     CDS             <1..400
                     /locus_tag="PLW82_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47124.1"
                     /translation="EQIRVALAGAFSEELVFGSMSTGSQNDFSQASRLAKTMVMAGLS
                     SLGIVAEELGQTEMNSEIMKIINEQGSFVREKIKPYKDFIQKIADYLLDKERIEGDRF
                     RDLLERYLQYSEIDVSDLQTGSAGLQAAVS"
     gene            1253..1960
                     /locus_tag="PLW82_07345"
     CDS             1253..1960
                     /locus_tag="PLW82_07345"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOJ47125.1"
                     /translation="MKRNLLFVLVLTVVAVLSIGVAAKMIDGTYSLNGPTDDHGNYPV
                     IKMVVKDGKIASVEYDEIIAAQGVPKSKDNYNWEPYFSAVEYLEKRAVEVNGDVSKID
                     AIAQATGSSVKFKDMLLAVQLQAVQLKDAIYPPVELEPDNRGYAPKLQITVSGGKISK
                     VEYYEYNTQNGTPKEEGVYNWPAYFEAIKYFGETAVKENGNIMNIDAFAGATSTSDKF
                     FALYRTLVNMILLVAKQ"
BASE COUNT          594 a          393 c          501 g          531 t
ORIGIN      
        1 agagcagatc agggtggctc ttgcaggggc attctccgaa gagttggtgt ttggctctat
       61 gagcaccggc agccaaaacg acttctctca agcatctagg cttgcaaaga ccatggtcat
      121 ggccggcctg tccagcttgg gtattgtggc agaagaacta ggccaaaccg aaatgaacag
      181 cgagattatg aagatcatta acgaacaggg cagcttcgtt agagaaaaga tcaagcccta
      241 caaagacttt attcaaaaga tcgccgacta cctcctggac aaagagcgta ttgaaggaga
      301 cagattccgc gatcttctgg aaagatactt gcagtacagc gagattgatg tctctgactt
      361 gcaaacaggc agtgcaggtc tgcaggctgc agtttcttaa ggatcaggaa tcccctgatc
      421 cttttttttg caggtaattt tagcactttt caaaaagtga agtagttaac aaaatatggt
      481 gtaaacaacc ttaagcaaga gatataccct tttggttagc tgtaggaagt gcaaagctct
      541 ttgcaccaaa ggccttaagt cctgtatatt tagtatgtag cattctgttt tgcttcagtg
      601 ctaactaagt ggagtgagag tagtggtaag gaggttttgt agtatttagg cttcgggtct
      661 aatggaatgg tcggcaaaat ccttccgggt tcgaagtgcc tggggaacag ctccagaccg
      721 cctttgcagg ccaggtcgcg actttctttg agaaattgat ctggaccttg atgtctggag
      781 atgaaaagcc caagactgtg tgcgataaaa ccgggatcaa acgggtataa tgtgactaaa
      841 gccacatcgt taaggatgat ataatgaaaa aacaccagaa gagccgcgcc tttaagtgcg
      901 tgaagaataa ctttgaaacc tatgaggaat acagacttcg agtacatttt tggctcgatc
      961 agctattaga tgaagagatt cgctttgcaa gacttgccgg acggcgcaag ttcaaggttg
     1021 tgggggttat tagtagttgg aaaggcgata atgatcggag ttagtacgcg tattgccctc
     1081 gcttgaagga aaaacgtagt atattcgctg cgggaaaacc aattccttct agatttgggt
     1141 gttctttgaa gttagttaaa tttttttcga agtctgaagg ataatactaa cgagcctaga
     1201 atattgtata ctgacaatta gttagtatga gtcaatctag gaggaacaaa agatgaagag
     1261 aaatctgcta tttgtcttgg ttttaacagt ggttgctgtt ctgtccatcg gtgttgcagc
     1321 aaaaatgatc gatggcacct attctctcaa tggtccaaca gatgatcacg gtaactaccc
     1381 agttatcaaa atggttgtta aagacgggaa gatcgcttcc gtagagtacg acgagatcat
     1441 cgcagctcag ggcgttccaa agtccaaaga caactacaac tgggagcctt actttagtgc
     1501 tgttgagtat cttgagaaga gagccgtaga agtcaacggt gatgtttcca agatcgatgc
     1561 tatcgcacaa gctaccggca gcagcgtcaa gttcaaggac atgctccttg cagtacagct
     1621 tcaagcagta cagctcaaag atgctattta ccctccagta gagttggagc cagataaccg
     1681 cggctacgct ccaaagcttc aaattactgt aagtggcggt aaaatcagca aggtcgagta
     1741 ctatgagtac aacactcaga atggaacccc aaaagaagag ggcgtttaca actggcctgc
     1801 gtactttgaa gctattaagt actttggtga aacagccgtt aaagaaaacg gcaatatcat
     1861 gaatattgac gcatttgctg gtgcaacaag cacttctgac aagttctttg cactctatag
     1921 aacactggtc aacatgatct tgttggttgc aaaacagtaa tgcgaaaggg gctgttgcac
     1981 aattagccga ttaagctgat tgtgcggcag ccttttttt
//
LOCUS       DAOSDA010000325         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_37530_length_1996_cov_3.584235, whole genome shotgun sequence.
ACCESSION   DAOSDA010000325 DAOSDA010000000
VERSION     DAOSDA010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37530_length_1996_cov_3.584235"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PLW82_07350"
     CDS             complement(<1..1137)
                     /locus_tag="PLW82_07350"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOJ47126.1"
                     /translation="MKLYLYKTSDQKDWRFVFPLENLPLGTYQIAGPFNYKKAVFLQK
                     AYRFLPHMARSFVYHRAAQPKLPQTPPRGIWRPYRSETGVYPNDIIKPGISRIRWRCV
                     RCGSGNIDTHQCPECGKTNCLVCRDCYMLGPIRGCRLYLWQSPGVPEYFARELKPQIP
                     FDLTPGQKSAGDALRQALAKGSRKLLLHAACGAGKTEVASLLIADALNCGKRVLYTVP
                     RRDVAREVGERLREYFPEVDCVILYGKSPEKYTDQRLVAATTHQVIRFYKSFDLAIID
                     EGDAFPYKGNDFLVRAVKRASRGPIVYMTATPTDEMLKEFKKSEVVFIPARHHKRALP
                     VPRFSSASLEKALEGVKPPLLVFVPYKKNVSAMVERVKRCKPDWK"
     gene            complement(1189..>1996)
                     /locus_tag="PLW82_07355"
     CDS             complement(1189..>1996)
                     /locus_tag="PLW82_07355"
                     /inference="COORDINATES: protein motif:HMM:NF037213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOJ47127.1"
                     /translation="PRIVDSGKIPVVKLTKVFRQKNQSQIVLNAHRINEGYFPEIDNN
                     GDFVFMERKAPEEISSLIVELVADRLPKYVRKRYNMTIDFDTIQVLTPMRKSETGVDN
                     LNKLLQKRLNPSGKKNEIQIGHRLYREQDKVMQIKNNYQKDVYNGDIGKIYRIDPEDK
                     VVDVLFTDETGDRYVRYEEDELDELTLAYAISVHKSQGSEYPVVVMPVTTQHYVLLQR
                     NLLYTGITRAKRLVVLVGSKQALFLGIKNNNTAQRYSYLAERIRMAVSSN"
BASE COUNT          429 a          527 c          474 g          566 t
ORIGIN      
        1 cttccagtcc ggtttacacc tttttaccct ctccaccatc gcagagacat tctttttgta
       61 aggcacaaac accaataggg gaggtttgac accttcgagg gccttctcca aagaagccga
      121 gctgaaacgc ggcacgggaa gggctctctt gtgatgcctt gcgggaataa agactacttc
      181 gctctttttg aactccttca gcatctcatc ggtgggagtt gctgtcatgt atactatggg
      241 tcccctggat gccctcttta ccgcccttac taaaaagtcg ttgcctttat agggaaaagc
      301 atccccctca tctataatcg cgagatcgaa agacttgtaa aatctgatca cctggtgggt
      361 ggttgcggcc acgagccttt gatcggtgta cttctccggg cttttgccgt agagaatcac
      421 gcagtcgact tcgggaaaat attcccgaag tctttcgccc acttcccttg caacatcgcg
      481 cctaggcacc gtgtacaaaa ctctcttccc gcagttcagt gcatctgcga ttaaaagcga
      541 tgctacttcc gttttccccg ctccacaagc agcgtgaagc aagagctttc tgcttccttt
      601 agctaatgcc tgcctcaacg catctcctgc gcttttctgg cccggagtca gatcaaaggg
      661 gatctggggc tttagctctc ttgcgaagta ctcgggaacc cctgggctct gccagaggta
      721 aagcctacaa cctctaattg gccctaacat atagcaatcc ctgcaaacta aacagttcgt
      781 tttcccgcac tccggacact ggtgagtgtc gatgttgccc gaaccgcagc gaacgcaccg
      841 ccaccggatc ctggagatcc cgggcttaat aatgtcgttc gggtacactc ctgtctcgga
      901 tctgtagggc cgccagatac cccgtggagg agtttgggga agttttggct gtgcagctct
      961 gtgataaaca aaactgcgtg ccatgtgcgg taaaaaccta taggctttct gcagaaagac
     1021 cgcttttttg tagttgaaag gtcctgctat ctgataggtt cctaaaggca gattctccaa
     1081 agggaaaaca aacctccaat ctttctgatc ggaggttttg tacagataca acttcacaga
     1141 actttcatct ccattcgtcg ggtgaaagct ctaggtgtgc ttcttttttt agttggaact
     1201 tactgccatt cttatgcgtt cggccaaata gctgtatctt tgggcagtgt tattgttctt
     1261 gatccccaag aacagagctt gcttgctgcc caccaaaacc accagacgct tggctctggt
     1321 aatgccggta tagaggaggt ttctctgcag gagcacgtag tgctgggttg ttacgggcat
     1381 caccactacg ggatactcgc ttccctggct tttgtgcact gagatagcgt aggccagagt
     1441 cagctcatcc agttcatctt cctcgtagcg tacgtagcgg tcaccggttt cgtcggtaaa
     1501 cagcacatca acaaccttgt cctcaggatc gatccggtag atttttccaa tatctccgtt
     1561 ataaacatcc ttttggtagt tgttcttgat ctgcatgact ttgtcctgct cgcggtatag
     1621 cctgtggcct atttggattt cgttcttttt accgctgggg ttcaagcgct tctggagcag
     1681 cttgttcagg ttgtcgacac ctgtttcgct cttgcgcata ggcgtgagca cctgtatggt
     1741 atcgaagtcg atggtcatgt tgtaacgttt tctcacatac ttaggtagcc tgtccgctac
     1801 cagttccacg atcagactgg agatctcttc aggcgctttc cgctccatga aaacaaaatc
     1861 gccgttgttg tcgatttcgg ggaagtaccc ctcgttgatt ctatgagcat taaggacaat
     1921 ctgcgactgg tttttctgcc tgaaaacttt ggtcagcttt accaccggga tcttccccga
     1981 gtcgacgatc ctgggg
//
LOCUS       DAOSDA010000326         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_37613_length_1993_cov_3.826625, whole genome shotgun sequence.
ACCESSION   DAOSDA010000326 DAOSDA010000000
VERSION     DAOSDA010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37613_length_1993_cov_3.826625"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLW82_07360"
     CDS             <1..530
                     /locus_tag="PLW82_07360"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOJ47128.1"
                     /translation="VDNVLQRTADPVFVGAVIEGNFSAGCKVVRKINPEENIGVLCME
                     DGKPSIVEYYELTDDMRYEKNDRGEYIYNFGTILNFLFKESELANLLEKELPIHVVER
                     KIPYIDENGNLVKPEQPNGYKYEQHINDMVHMIGSCLPFEVLREHEFAPIKNKEGIDS
                     VETARELLRKNGVIL"
     gene            915..1454
                     /gene="hpt"
                     /locus_tag="PLW82_07365"
     CDS             915..1454
                     /gene="hpt"
                     /locus_tag="PLW82_07365"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOJ47129.1"
                     /translation="MHPDVEKIIISQEQIQNRVKELGRQLTEDYRGKELVVIGILRGA
                     AVFLADLIREIDLYMEIDFMGVSSYGLATKSSGVVRITKDLESTITDRDVLIVEDIVD
                     SGLTLKYIVENLRSRKPRSIQICTLLDKPARRVEGVEVAYNGFVIPDEFVVGYGLDYA
                     EKYRNLPYVGVLKPEIYTT"
     gene            1539..>1993
                     /locus_tag="PLW82_07370"
     CDS             1539..>1993
                     /locus_tag="PLW82_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47130.1"
                     /translation="MKDTYAYETVLVIDFGSRKSQDVARMIRKQHVYSEVFSYTKALE
                     AINEKSPKGIVLCEEGNADLVALLEKKAEELGIPVLKTTEELDLDALNTSEAEQTVKD
                     FLFVRCGVQASWTMSAFVEKATDDIRKQVGDGKVICGLSGGLDSAVTALL"
BASE COUNT          590 a          375 c          519 g          509 t
ORIGIN      
        1 ctgtagacaa cgttcttcag agaactgcag atcctgtatt tgtaggtgcc gttatcgaag
       61 gtaacttcag tgcaggttgt aaggttgttc gtaagattaa tccagaagaa aacatcggcg
      121 ttctgtgtat ggaggacggc aaaccgtcca ttgtcgagta ctatgagctt actgacgata
      181 tgcgttacga gaagaacgat aggggcgagt acatttacaa cttcggtact atccttaact
      241 tcttgtttaa ggagtcagaa cttgctaatc ttcttgagaa agagcttcct attcatgtgg
      301 tagagaggaa gattccttac attgatgaga atggtaacct ggtcaaacct gagcagccaa
      361 acggctacaa gtatgagcag catattaatg atatggttca tatgattggc agttgtctac
      421 catttgaggt gcttagagag catgagtttg ctccaatcaa gaacaaggaa ggcattgact
      481 cagtagagac agccagagag ctcctaagga aaaatggtgt aattctttaa cagaacatag
      541 agcggctttg tttgccccac ctttgcaggt gacttagtgt gaaaggcatt tcagaacaag
      601 tctgattggg tatataatga gtcaaagaac attgtggctc tcgaggagcc ggtgtgtctg
      661 tagaaagcgg cattccgtac cttagaagta agaacaggtt atccaagtag gactagtgga
      721 gtttcagcga gaacagctgg agcacccgct ggcaagagac ggttaatacc tggtttggaa
      781 gagttcttgg tttatttacg caaaaacagt atcttgcggg aaggaatatg ctatggttaa
      841 ggtgtatatt cctatattct aacgatctga attcgcaaaa ctagatggaa ggagtagtac
      901 ttttgagtaa cgttatgcat cccgatgtgg aaaaaatcat tatttctcaa gagcaaatac
      961 agaaccgagt gaaagaactt ggcaggcagc tgactgaaga ctacagaggc aaggaattgg
     1021 tggttattgg cattttgcgg ggcgctgcag tgtttttggc cgaccttatt cgcgagatcg
     1081 atttgtacat ggaaattgac ttcatgggtg tatccagtta cggattggca acgaaaagtt
     1141 caggtgttgt gcgcatcacc aaagacctgg agtcaacaat aactgataga gacgtactta
     1201 tcgttgaaga cattgtagac agcggtctga ccctcaaata cattgttgaa aacctcagat
     1261 ccagaaaacc aaggagcatc cagatttgca ccttgctgga caagcctgca cgcagagtag
     1321 aaggtgtgga ggttgcgtac aacggttttg taattcccga tgagtttgta gttggttacg
     1381 gtctcgacta tgcagagaag tataggaacc ttccctatgt tggggtgttg aaaccagaga
     1441 tatacaccac ataaaaagcg atcattatat accgaaaaaa aggagggata gctgcatcga
     1501 ttctcaagca aagaagcggg ggtcatgcag tatcttacgt gaaagacact tacgcttatg
     1561 aaactgtcct tgtaatcgac ttcggttcgc ggaaaagcca ggatgttgcc cgcatgatca
     1621 ggaaacagca cgtgtacagc gaagtcttct cctacaccaa agctttggaa gctatcaacg
     1681 agaaaagccc caaaggcatt gtgctttgtg aggaaggcaa cgcagacctg gttgcgttac
     1741 tggagaaaaa agccgaagaa ctcggcattc cagtgcttaa aaccacagaa gaactcgatc
     1801 tggatgcgct gaatacgtct gaagcggaac agacagttaa agattttctg tttgtcagat
     1861 gcggggtgca ggcatcctgg actatgagtg cttttgtgga aaaagctaca gacgacatta
     1921 ggaaacaggt cggagacgga aaagttatct gcggtctcag cggcggcttg gattcggcgg
     1981 taactgccct tct
//
LOCUS       DAOSDA010000327         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_37854_length_1984_cov_3.319855, whole genome shotgun sequence.
ACCESSION   DAOSDA010000327 DAOSDA010000000
VERSION     DAOSDA010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37854_length_1984_cov_3.319855"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1984)
                     /locus_tag="PLW82_07375"
     CDS             complement(<1..>1984)
                     /locus_tag="PLW82_07375"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOJ47131.1"
                     /translation="HTEDSLLTCGAEVQKGNPIEERKITRLFRRPEVSKLIKKSNDFG
                     AGGVSVAIGELADSLDIYLDRVKVKYRGLNATEIAISESQERMAVVIAKKDQEEFERY
                     CNEENLEVVHVADVTDTGRLRMFFEGRECVNLKRSFLDSAGATHYTKVAVGSVDEKDP
                     FVREVPGKTLKEKVLNNLSDRNVLTQKGLVQMFDSTAGASTVLMPYGGKYQASETQVS
                     VQKLPAEGSTDLCSIMAYGYNPEISKWSTYHGSQYAVVEAIAKIVAAGADYRNIRFSF
                     QEYFQKMTDEYAWGLPFAALLGAIRMQDAFKLPSIGGKDSMSGTFKDIHVPPMLMAFG
                     ITTVSAKEVISTDFKAEGNYIYLIRHNPLESYSPNTEELKENFGKVREAITDGRIVSA
                     YAVGFGGISEALAKMSFGNSIGFEVEAAEQDLFACSYGSIVVESKEELPFGELLGRTS
                     SKWLINGTPFTLDELWKANTAKYGEIYNYRTDKSGKVEAPAAPKREIAYPGPKKTPTV
                     YVPVFPGTNGEYEIARAFKKAGAEVVTTVFKNLTADDVRVSLDEVARTIAQSDILVVC
                     GSFSLDSDPEWSAKFISGVLLDGLVADQIRKLLERKGLILGIASGFKALLDTGLLPYG
                     RLGEVGEDSPTLFRNDLGGHVSQMVSTRVASTNSPWL"
BASE COUNT          414 a          541 c          499 g          530 t
ORIGIN      
        1 cagccacggt gagttggttg aagccactct ggttgaaacc atctggctca catgaccgcc
       61 gaggtcgttt ctaaaaagcg ttggcgagtc ttctcctacc tctcccagtc tgccatatgg
      121 cagaagtcct gtatcgagga gggctttgaa gccgctggca atgccgagaa tcagtccctt
      181 gcgctctagg agcttgcgga tctggtcagc tactagcccg tccagtagga cccctgaaat
      241 gaactttgcg gaccattctg gatctgaatc cagactgaag ctgccgcaga caaccaggat
      301 atccgattgg gctatggtcc gtgctacttc atctagagaa acacgaacat catctgccgt
      361 caggttcttg aaaacagttg tcacaacttc tgcaccggct ttcttgaaag ccctggctat
      421 ctcgtattcg ccgtttgtac ctgggaaaac cggcacgtaa actgttggag tctttttagg
      481 tccgggatat gcgatttccc tctttggggc cgctggtgct tccactttcc cggatttgtc
      541 tgtacggtag ttgtagatct ccccgtactt tgcggtattt gccttccaca gctcatcaag
      601 ggtaaacggc gtgccgttaa tcagccactt tgaactggtt ctgcctaaaa gctcgccaaa
      661 aggcagctcc tctttggact cgaccacaat tgagccgtag ctgcaggcaa acaggtcctg
      721 ttctgcagct tctacttcaa agccaatgct gttaccgaag ctcattttgg ccagtgcttc
      781 agaaatgccg ccaaagccca ctgcataagc gctgacgatc ctgccgtctg ttatcgcctc
      841 gcgcactttg ccgaagttct ccttcagctc ttcggtgttg gggctgtaac tttctagcgg
      901 attgtgccta atgaggtaga tgtagttgcc ttcggctttg aagtccgttg agatgacttc
      961 tttggcgctt acggtggtga tgccaaaggc catcagcatt ggcggcacgt ggatatcttt
     1021 gaacgttccg gacatggagt ccttaccccc tatggaaggg agtttgaacg catcttgcat
     1081 tctgatggct ccaagtaagg ccgcaaaagg caaaccccat gcgtactcgt ctgtcatctt
     1141 ctggaagtac tcctggaagg agaacctgat gtttctgtaa tctgcacctg cagctacgat
     1201 cttagcgatg gcctctacca ccgcatactg gctaccgtgg taggtgctcc acttgctgat
     1261 ttcagggttg tagccgtagg ccatgatgga gcagaggtcg gtgctgcctt ctgcaggcag
     1321 tttctgtacc gagacctgag tttccgatgc ttggtacttg ccgccgtagg gcatgagaac
     1381 tgtagaagcc cctgcagtgg aatcgaacat ctgcaccaag cccttttggg tgagaacgtt
     1441 gcgatcggag aggttgttca ggactttctc ctttagagtc tttcccggca cctcccggac
     1501 gaagggatcc ttttcgtcaa cactaccaac ggcaaccttc gtgtagtggg tcgccccagc
     1561 gctatccagg aagctgcgct tgaggttcac gcattctctg ccttcaaaga acatcctcag
     1621 cctaccggtg tctgtgacgt ctgcaacgtg gacaacctcc aggttctcct cattgcagta
     1681 tctctcaaac tcttcttggt cctttttggc aataacaacc gccatccgtt cttgagactc
     1741 ggagatagca atctctgtag catttaagcc ccggtacttg accttaactc ggtccagata
     1801 gatgtccaga ctgtctgcaa gctcgccgat ggcaacagag acaccacctg cgccgaagtc
     1861 gttggacttt ttgatcagct tggagacctc cggtcttctg aacaagcggg taatcttgcg
     1921 ctcttcaatg ggattgccct tctgcacttc ggctccgcaa gttaaaagag aatcctcggt
     1981 gtgt
//
LOCUS       DAOSDA010000328         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_37914_length_1982_cov_3.117800, whole genome shotgun sequence.
ACCESSION   DAOSDA010000328 DAOSDA010000000
VERSION     DAOSDA010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37914_length_1982_cov_3.117800"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLW82_07380"
     CDS             <1..416
                     /locus_tag="PLW82_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOJ47132.1"
                     /translation="VTDIVEIALAVEDAGADALTLINSLTGMTIDVRSKKPLLGNITG
                     GLSGPAIRPIAVRMVYQVAGRVKIPVIGVGGITCLEDALEFFMAGASAVQVGSGLFRD
                     HTLLERLPSEFEAYLEEEGLTAEELVGIARRNVNA"
     gene            409..1095
                     /gene="pyrF"
                     /locus_tag="PLW82_07385"
     CDS             409..1095
                     /gene="pyrF"
                     /locus_tag="PLW82_07385"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOJ47133.1"
                     /translation="MLDRIYVALDYPSRMEADRLLDRLPQGVGVKIGLELFLREGLSY
                     VREIKYRGYNVFLDLKFHDIPRTVYGAVSQVADSCDLLNVHAAGGLEMLKAAKEASLK
                     AEKPPKLIAVTVLTSLNQSDWQEIGGAGALAQGAEKRALLSREAGLDGVVYSPLEAAR
                     IKALCGEEFLTVTPGIRPQSAASQDQKRTATPREAVEMGCDYLVVGRPITKAEDPARA
                     LFEIVEEMKS"
     gene            1092..1658
                     /gene="pyrE"
                     /locus_tag="PLW82_07390"
     CDS             1092..1658
                     /gene="pyrE"
                     /locus_tag="PLW82_07390"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOJ47134.1"
                     /translation="MTEIKAIFQESGALLSGHFKLTSGKHSGQYVQCARVLENPEITQ
                     KLVAKWVERLQGVPVDVVIGPAVGGIILSYELGRQLGKKAIFAEREGGKLTLRRTFAI
                     EPGERVVVVEDVVTTGGSVLETMEVVKQAGGTVEAVCALVDRGSGFDPGTLYVPLLTI
                     DIASYLPEECPYCREGLPLTAPGSRSLK"
     gene            1758..>1982
                     /locus_tag="PLW82_07395"
     CDS             1758..>1982
                     /locus_tag="PLW82_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOJ47135.1"
                     /translation="MTVFKNCSIVLPERVIPGYVVVEDGLIAEIGFGESPYDGVDFHG
                     DYLVPGYIDVHVHGAVGHSFHVGDEVGSSKV"
BASE COUNT          505 a          408 c          603 g          466 t
ORIGIN      
        1 atgtgaccga tattgttgaa attgcacttg ccgtggaaga tgccggagca gacgcactga
       61 ccctcattaa ctcgctaaca ggaatgacca tcgacgtgag gagtaagaag cccctgcttg
      121 gcaacatcac gggaggcctt tccggtcctg ccatcaggcc tatcgccgta aggatggtgt
      181 atcaagtagc aggaagagtt aagattccgg tcatcggcgt agggggaatc acctgtctcg
      241 aggatgcgct ggagttcttt atggctggtg ccagtgcggt acaggttggt tccggactgt
      301 ttagagatca tactcttctc gaaaggttgc cctcagagtt cgaagcttac cttgaagaag
      361 aagggctcac cgctgaagaa ctggtgggta tcgctaggag gaatgtgaat gcttgatcgc
      421 atctatgtag cactggacta cccgagcaga atggaagcgg atcggttgct ggaccggctg
      481 cctcaaggtg tgggagtaaa gataggtttg gaactctttc tccgagaggg attgagctat
      541 gttcgtgaga tcaaataccg gggatacaac gtgtttttgg atctgaagtt ccacgacatc
      601 cccaggacag tctacggtgc ggtatctcag gtggcagaca gctgcgacct cttgaatgtt
      661 catgctgcag gagggttgga gatgctcaag gccgctaaag aagcgtcgct gaaagcggaa
      721 aaaccaccca agctaattgc ggttacggtt ctgacgtctc tgaaccagag cgattggcag
      781 gagatcggag gagccggtgc tctagcacaa ggggcagaaa agagggccct actttctcgg
      841 gaagcaggtc ttgacggtgt tgtctattcg cctctagagg cagcgaggat caaggcgttg
      901 tgcggtgagg agtttttaac agtcactccg ggtatcagac cccagtccgc agcaagccag
      961 gaccaaaaac gcactgccac tccaagagaa gccgtagaga tggggtgcga ttacctggtt
     1021 gtgggcagac ccataactaa agctgaagat cctgccagag cgctttttga gattgtggag
     1081 gagatgaagt catgacggag atcaaagcga tttttcagga atcaggtgcg cttttgtcgg
     1141 gccactttaa gctcactagt gggaagcaca gcggtcagta tgttcaatgc gcaagggtgc
     1201 tagagaaccc cgaaatcact cagaagctgg tggcaaagtg ggtagaaaga ctccagggag
     1261 tacctgtaga tgtggtcatt ggacctgcag ttggcggtat aatcttaagc tatgaactgg
     1321 gcaggcagtt aggtaagaaa gcgatctttg ctgaaagaga aggcggaaaa ttaaccttaa
     1381 gaaggacttt cgccatcgag cctggcgaaa gagtggtagt tgtggaagat gtagtgacaa
     1441 ccggcggctc ggttctcgag actatggagg ttgtaaaaca ggcaggaggc acggtggaag
     1501 ccgtttgtgc cttggtggat agaggcagcg gattcgatcc gggaacgctg tacgtgcctc
     1561 ttttgaccat cgatattgca agctaccttc ctgaagaatg tccctactgc agggaggggt
     1621 taccccttac agcaccgggg agtagaagtc tgaaataaaa cggcggtgat tagttctagg
     1681 tttacgtcgc cgaagaatgg accatatgtt actataaaaa gtgaagagag tctgtagact
     1741 tcagggaggt gccgtaagtg actgttttta aaaactgcag cattgttctg ccagagagag
     1801 tgatacccgg ttatgtagtg gttgaagatg ggttgattgc agagatcgga tttggtgaat
     1861 ctccctacga tggagttgat tttcacggtg actatctagt tccgggctat atcgacgtac
     1921 atgtgcacgg agcagttggc cattcttttc acgtaggcga cgaagtaggt tctagtaaag
     1981 ta
//
LOCUS       DAOSDA010000329         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_38472_length_1963_cov_2.751048, whole genome shotgun sequence.
ACCESSION   DAOSDA010000329 DAOSDA010000000
VERSION     DAOSDA010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38472_length_1963_cov_2.751048"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLW82_07400"
     CDS             complement(<1..235)
                     /locus_tag="PLW82_07400"
                     /inference="COORDINATES: protein motif:HMM:NF015783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HOJ47136.1"
                     /translation="MEQKTQVPSNQIVSLVTVFLIGTITIISPANEMGKYSYISVLLA
                     FLIYLVVLGLYYLLSSMEPDKNLFAIHDAAYGRI"
     gene            complement(250..1371)
                     /locus_tag="PLW82_07405"
     CDS             complement(250..1371)
                     /locus_tag="PLW82_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOJ47137.1"
                     /translation="MKRAVLFLLIVFALAGCYDRVDLNEVEIVVAMGLDVAEDGRVKA
                     TVQVLNPSPANSIVSGGEPFAKIESVANSFTEAVRKANLYSPNRLYFAHNRLVIFSED
                     FVNTHGTHHVIDWIMRTDDAREDTFILITSGKASDILASKFPTGSIPAFEITQIVLTN
                     YVNSLAVVTTAFDYAKDRASQIPMTVMGRVEQIKEEDGSLIVYKGAGLIHPHQFMDYL
                     TETETKGYNWLTGRFGTGLIVATVKGGSEKFTYEIVRNRTSLTPKLVDGQLTFVFSAE
                     VTGRITKLESSKSLNDPFSWPEYLHAIEEGIKREVRAAVDKAKEQGDFIGLGHLVMRR
                     LPKFWRENEAQWEQIWPEAPFVFDIKVEINGVGKLIRFL"
     gene            complement(1368..>1963)
                     /locus_tag="PLW82_07410"
     CDS             complement(1368..>1963)
                     /locus_tag="PLW82_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOJ47138.1"
                     /translation="SPSLYIILVSFQHELLPTPLLVTFANAVEGTPFPAVVEVGVMMF
                     ILEIIREAGVRMPRPIGPAVGIVGSLVMGESAVSAGLIGQPLVIVIALTAITSYIVPT
                     LYDESALLRILFYVFTSFFGIIGFVTATVLIVIHLASLQPFGVPFMSPFMPFEGEDIK
                     DSFLRAPLWLMKRRPQVLGGKKRFGERPKSSIKGEDL"
BASE COUNT          484 a          483 c          528 g          468 t
ORIGIN      
        1 tgatcctgcc gtaagcagca tcgtggatgg caaaaaggtt tttatccggt tccatagagg
       61 acaaaaggta gtagagcccc aaaaccacaa ggtagataag gaatgccagg agcacactga
      121 tgtagctgta cttgcccatt tcgtttgcag gcgaaatgat ggtgattgta ccaatgagaa
      181 agactgtgac caaagacact atttggttgc tcggtacttg ggttttctgc tccacctgca
      241 aagtctccct tacaagaatc ttatgagctt gcctacgccg ttgatttcaa ctttaatgtc
      301 gaaaacaaag ggtgcctcag gccagatctg ttcccactgg gcttcgttct cccgccaaaa
      361 cttaggcagc ctccgcatta ccaagtgtcc gagaccgatg aagtcaccct gctctttggc
      421 tttatctacc gctgcacgaa cctcccgttt gataccttct tcaatggcgt ggaggtactc
      481 aggccacgaa aaaggatcgt ttagggattt agatgactcc agcttggtaa tcctcccggt
      541 aacttcggcg gagaacacga aagtaagctg accgtctacg agcttaggcg tgaggctagt
      601 gcgattgcgt acgatctcgt aggtaaactt ctcgctgccg cctttgacag ttgctacgat
      661 caagccggtg ccgaacctac cggtcagcca gttgtacccc ttggtttcgg tttctgtgag
      721 atagtccatg aactgatggg gatggattag ccccgcacct ttgtagacga ttaaactacc
      781 gtcttcttcc ttgatttgct caactcgccc cattacggtc atggggatct gtgatgcccg
      841 gtctttggcg tagtcaaagg cagtcgtcac cacagcaaga gagttgacgt aattggtcag
      901 cacgatttgg gtaatttcaa aagcggggat cgatccggtg ggaaactttg aggcgaggat
      961 gtcgctggcc tttccactgg tgatcaggat gaaggtatct tctctagcat cgtctgtgcg
     1021 catgatccag tcgatgacgt ggtgggtgcc gtgggtgttt acgaagtctt cgctgaatat
     1081 taccagacgg ttgtgggcga agtacaacct gttgggcgag tagaggttgg cctttcttac
     1141 cgcttctgtg aagctgttgg cgaccgactc gatcttggca aaaggctctc cgccggagac
     1201 gatgctgttt gctggagagg gattcagaac ttggactgta gccttaactc ggccgtcttc
     1261 tgcaacgtcg agccccatcg ccacaacgat ctccacctca ttgagatcaa ccctgtcgta
     1321 gcagcctgca agggcaaaaa caatgagcag aaaaaggaca gcccttttca taggtcttct
     1381 cctttgatag acgattttgg tctttcgcca aagcgcttct tgccacctaa gacctgaggt
     1441 cgccgtttca tgagccacag cggcgcgcgg aggaaagagt ccttgatatc ctcgccttca
     1501 aagggcataa acggcgacat gaagggaacg ccgaaaggct gtagacttgc tagatgaatg
     1561 acgatgagaa ctgtagcggt gacaaagcca ataatgccaa aaaacgaagt aaacacgtaa
     1621 aacaaaatgc gcagaagggc tgactcatcg tagagcgtgg gaacgatgta ggaggtgatg
     1681 gcagtaaggg caataacgat caccaggggt tggccgatca agccagcact tactgccgat
     1741 tcgcccatca ccagagagcc tacaatacct acagctgggc cgatgggtct tggcatccgc
     1801 accccggcct ctctgatgat ctccaggatg aacatcatca ctcctacctc taccactgca
     1861 gggaagggcg tgccttcaac tgcgttggca aaggttacaa ggagaggtgt gggcaggagt
     1921 tcatgctgga agctcaccag aatgatgtag agggaagggc tga
//
LOCUS       DAOSDA010000330         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_38615_length_1958_cov_3.167105, whole genome shotgun sequence.
ACCESSION   DAOSDA010000330 DAOSDA010000000
VERSION     DAOSDA010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38615_length_1958_cov_3.167105"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLW82_07415"
     CDS             <1..522
                     /locus_tag="PLW82_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOJ47139.1"
                     /translation="LKVRAEIENAKKHFEETGRNLFLDIMEEEKRFTQRVVYENDHFV
                     AFIPFFTDYPFGTFVLPKRQVLDLPELTREEEQSLAVLLKELVGGMDEIYSRLFPYMM
                     VMHPAPHKLKEEAKNYYWFHIEFYPPLRGEKSIKYNASSETGGWAAANPTKVEDNAAI
                     FREAIERYRRRDK"
     gene            522..1703
                     /locus_tag="PLW82_07420"
     CDS             522..1703
                     /locus_tag="PLW82_07420"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOJ47140.1"
                     /translation="MGSLLEQLKQEFKEFYPEAGSPRVFFAPGRVNLIGEHTDYTGGF
                     VMPMALDLGITMLTAPRKDGVFRLRSKSFEQPVEFSIEDLKDDSKADKKDTWGNYPKG
                     VVIELLKLGAPVTGADILYSSNLPTGAGLSSSAAIGMATAFGLAEVAEFEVSKQELAF
                     LCQRMENNFIGVKSGIMDQFAVGLSKENHAIFLDCTSIEYEYVPFELEDHKVVITNTA
                     KKRGLNESRYNERREEAEEALKQIAEMKPNVKSYRDLSLDDLSLINALRYPYRERARH
                     IVTENHRVLEAKAALEQGNLTQFGQLMLASHRSLSLDYEVTGKELDTLFQVQLEAGCY
                     KTRMTGAGFGGCTVSLVKEDEIDQFIEYVRANYKERTGLDPEFYIAKGSGGVREIGEE
                     D"
     gene            1704..>1958
                     /locus_tag="PLW82_07425"
     CDS             1704..>1958
                     /locus_tag="PLW82_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005581845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ47141.1"
                     /translation="MSILVTGGAGYIGSHTVKYLRSLNKDVVVLDDLSKGHREAVLDA
                     PLYVGSIQDGDLIDSICNKHHVTGVIHFAAYSLVGESVKEP"
BASE COUNT          550 a          461 c          521 g          426 t
ORIGIN      
        1 ttgaaggtga gagccgagat cgaaaacgcc aagaagcatt ttgaggagac cggaaggaac
       61 cttttcttgg acatcatgga agaagaaaag cgcttcacac agcgcgtagt gtacgagaac
      121 gaccactttg tggccttcat tccctttttc accgactatc ctttcgggac atttgttctg
      181 cccaaaaggc aggttctcga tcttccagaa ctcaccagag aagaagaaca gtccctggca
      241 gtgctcctga aagagctggt gggtggcatg gatgagatat acagcaggct tttcccctac
      301 atgatggtga tgcatccggc accccacaag ttgaaggaag aagcgaagaa ctactactgg
      361 ttccacatcg agttctatcc tcccctccga ggagaaaaga gcattaagta taacgcttca
      421 tctgaaaccg gcggctgggc cgctgccaac ccaacaaaag tcgaagataa cgcagcgatc
      481 ttcagagaag caatcgagcg ctacagaagg agagacaaat aatgggaagt ttgctggaac
      541 agctgaaaca agagtttaaa gagttctatc ccgaagcggg ttctccgaga gtcttctttg
      601 ctccgggcag agtcaacctc atcggcgaac acaccgatta cacaggcgga ttcgtaatgc
      661 ctatggctct tgacctcggc attaccatgt tgaccgcacc acggaaggac ggagtcttcc
      721 gtttgagaag caagagtttt gaacaaccag tggagttttc tatagaagac ctaaaagatg
      781 actcaaaagc ggacaaaaag gacacttggg gcaactatcc caaaggagtg gttatagaac
      841 tactaaagct cggagcacct gtgaccggtg ccgatattct ctacagtagc aacctaccga
      901 caggcgcagg cctttcatct tcagctgcta tcggaatggc tacagctttt ggccttgcag
      961 aggttgcgga gtttgaagtt tccaaacaag agctggcatt tttgtgccag cgaatggaga
     1021 acaacttcat cggggtaaag agcgggatca tggatcagtt tgccgtgggt cttagcaaag
     1081 agaaccatgc gatctttctc gactgtacgt ccattgaata tgagtacgtg ccttttgagc
     1141 ttgaagatca taaggtggta atcaccaata cagctaaaaa acgtggactg aacgagtccc
     1201 gttacaacga aagaagagaa gaagcggaag aggctctcaa gcagatcgca gagatgaagc
     1261 cgaatgttaa gagttatcgc gacctgagcc ttgatgacct cagtctgatc aatgctctga
     1321 ggtacccata cagagaaagg gcaaggcaca ttgtcaccga aaatcaccgc gtgttggaag
     1381 ccaaagcggc tctggaacaa ggcaatctaa ctcagttcgg ccagctgatg ctggcatccc
     1441 acaggtctct ttccttggac tacgaagtta ccggaaaaga gctggatacc ctcttccagg
     1501 tacagcttga ggccgggtgc tacaaaaccc gcatgaccgg cgcaggcttc ggtggttgca
     1561 ccgtcagctt ggtcaaggaa gacgagatcg atcagtttat cgagtacgtg cgggccaact
     1621 acaaagaaag aactggcctt gatcctgagt tctacattgc caaaggcagc ggaggcgttc
     1681 gggagatagg agaggaagac tagatgagta ttctggtaac cggtggcgcc ggttacatcg
     1741 gcagccacac tgttaagtac ctgagaagct tgaacaagga cgtggtagtc ttggatgacc
     1801 tttccaaagg ccacagagaa gcggtgctag atgcacctct gtacgttggc agcattcagg
     1861 acggggatct catcgactcg atctgcaaca agcaccacgt taccggtgtc attcactttg
     1921 ctgcctattc tttggtcggt gagagcgtga aggaacct
//
LOCUS       DAOSDA010000331         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_38902_length_1948_cov_3.151611, whole genome shotgun sequence.
ACCESSION   DAOSDA010000331 DAOSDA010000000
VERSION     DAOSDA010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38902_length_1948_cov_3.151611"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PLW82_07430"
     CDS             <1..658
                     /locus_tag="PLW82_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ47142.1"
                     /translation="KRSHWLIGGDGWAYDIGFGGLDHVLSTGEDVNILVMDTEVYSNT
                     GGQSSKATQTAAVAKFAASGKRIRKKDLGLMATTYGYVYVAQIAMGANMNHALKTIRE
                     AEAYKGPSLIIAYSPCINHGLKNGMGMSIRQEQKAVEAGYWHLWRYNPELKEQGKNPF
                     ILDSKEPTGSFIDFLRSEIRYSSLERTFPEEAQELFALAEKHAKERYETYKRLASIEY
                     "
     gene            complement(728..898)
                     /locus_tag="PLW82_07435"
     CDS             complement(728..898)
                     /locus_tag="PLW82_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HOJ47143.1"
                     /translation="MNKQKNLTKIKVIRPIENHQTAAWAGVKKLAPETRIIIPEEEMV
                     LQAKEYVDENQK"
     gene            1305..>1948
                     /locus_tag="PLW82_07440"
     CDS             1305..>1948
                     /locus_tag="PLW82_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOJ47144.1"
                     /translation="MSIQSGPEALNEYILRLGQKKAEKKPRMLLIQGLLAGLYIAIGA
                     IASFRLSASIHVPGLGDFLGALVFPVGIIAVIVMQAELFTSNCMITTSTFAGEIKLAK
                     VLKILGLVLLGNLLGSICAAFLAQVSGIMDSAAHIVVSKALHKVNIPPVKLFVSAVFC
                     NIVVCTGVCLAYNLKDEMAKLTALWLAITVFVITGTEHVVANMYYLFAAVFAGAP"
BASE COUNT          485 a          474 c          455 g          534 t
ORIGIN      
        1 caagcgttct cattggctca tcggtggaga cggttgggct tacgacatcg gcttcggcgg
       61 actagaccac gtgctgtcca ctggtgaaga tgtcaacatc ctcgtcatgg acaccgaagt
      121 ctactccaac accggcggtc agtcgtcgaa agctacccag acagctgctg tagctaagtt
      181 tgccgcaagc ggtaagcgca tccgcaagaa agacctcggt cttatggcca caacctacgg
      241 ttacgtctat gtagctcaga tcgctatggg cgccaacatg aaccatgctt tgaagaccat
      301 ccgcgaagca gaggcctaca aaggaccatc tctcatcatc gcctactcac catgtatcaa
      361 ccacggtctc aagaacggta tgggcatgag catccgtcag gagcagaagg cagtggaagc
      421 cggctactgg catctgtggc gctacaaccc agagctcaaa gagcagggta agaacccatt
      481 catcctcgat tccaaagaac caacaggttc cttcatcgac ttcctcagaa gcgaaatccg
      541 ctactcttca ctggagcgga cattcccaga ggaagctcag gagctcttcg ctctggccga
      601 gaagcacgca aaagagcgct acgaaaccta caagcgcctg gcatcaatcg aatactgagt
      661 ctaaaagacg atcccgatat acgaaaaatc ggacttctcc ggaagtccga tttttcaatt
      721 ctctatgcta cttttgattt tcatcgacat attcttttgc ctgcagtacc atttcttctt
      781 cgggaatgat aatgcgagtt tcaggagcga gctttttcac accggcccac gcagcagtct
      841 gatggttctc aattggtcgg atcactttaa tcttcgtgag gtttttctgc ttgttcatgt
      901 tactcctcct tcgttatatt atggggtcag gaaagagttc ttatccaact agacggtgta
      961 gagtgttccg gtagtggtag gtgcaaagcg aacatctgtt aagtacagaa ccaatagatg
     1021 ttatgtagta catatcactg tctaaggttc gtgtaaacct ttccttgctt ctggttccat
     1081 ttagaataca gctttttggc atttttgtaa agtttgtcac agtttatgac aaggttctgt
     1141 caaatttccg tctggaaatg gcagcatctg gacgattttc atcacaccct aggctttcaa
     1201 aaaaccttga ggcacaagtg cttttgtgtt gcccctcgaa atatggtatt atagcatttg
     1261 gtgcgctttt tgcacggact ttaaccatta gaaagggttt atctatgagt attcaatcgg
     1321 gaccagaagc gttaaacgag tacattttga ggctgggtca gaaaaaggcg gagaaaaaac
     1381 cacggatgct ccttattcaa gggttactcg caggtttata tatcgccatt ggtgccatcg
     1441 caagcttcag gctttcagca agtatacatg tgccgggctt gggtgatttt ctcggtgccc
     1501 tggtgttccc tgttggaatc attgctgtta tcgtcatgca ggcagagctt ttcaccagca
     1561 actgcatgat cacaacctca acctttgcag gggaaatcaa gcttgccaaa gtcctgaaga
     1621 tcttaggact agttctcctt ggcaatctcc tcggctcgat ttgtgcagct tttctagctc
     1681 aagtctccgg gattatggac tctgcagcac atattgtggt aagtaaagcg ttgcacaagg
     1741 taaacatccc gccggtaaag ctttttgtca gtgcggtttt ttgcaacatc gttgtctgca
     1801 ctggggtctg tcttgcctac aacctgaaag acgaaatggc aaaactcact gccctttggc
     1861 ttgcaatcac tgtgtttgta atcacaggaa cggaacacgt cgtcgctaat atgtactacc
     1921 tctttgcggc ggtttttgct ggagcacc
//
LOCUS       DAOSDA010000332         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_38965_length_1946_cov_3.448969, whole genome shotgun sequence.
ACCESSION   DAOSDA010000332 DAOSDA010000000
VERSION     DAOSDA010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38965_length_1946_cov_3.448969"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLW82_07445"
     CDS             <1..391
                     /locus_tag="PLW82_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOJ47145.1"
                     /translation="QMGQNIFKFVANMAPAFGMLGTLIGLIQMLTTIDNPDAIAAAMG
                     VALITTFYGSLLVNVVATPLAGKLAIRSRNEVLLKELMVEGILSLQAGENPRIVEEKL
                     KSFLSPQSRNGLQEFRRDREQVMRSAS"
     gene            435..1160
                     /locus_tag="PLW82_07450"
     CDS             435..1160
                     /locus_tag="PLW82_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOJ47146.1"
                     /translation="MTTWGDMMSLLLCFFILLYSFSSLDNQRFREIMVAFKNMAGFLD
                     GGTNLIPEGYNSEELSPFADSSQIQLQSLEALAAVIQDLLEEEGFVDQVELSLEDRGL
                     VMRFKDVVLFDIGSDALKEAPKEMLKKLSVILEKVPLPIRIEGHTDNVPINNARFPSN
                     WELSVARAVKVVRFLIEEGSLDSERLSALGYGEYRPIESNATPEGRNRNRRVDIIVLH
                     PSLAGSEPGAEWLEELSRTYPVK"
     gene            1181..1645
                     /locus_tag="PLW82_07455"
     CDS             1181..1645
                     /locus_tag="PLW82_07455"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOJ47147.1"
                     /translation="MAEKGKIVVDAKFVVFLIALVILATSGSALLTSRLLAQPKVVEA
                     VEDKPEFGPRVEFDAITVNLQNGVLGQKAPLIRVKLVVELGTEKIPDDFDVYKPVLQD
                     KVIELLRSKTATDLTGGEGQIRLRQEIRKAFNEILPREPVTSVYFTEFLIGN"
     gene            1665..>1946
                     /locus_tag="PLW82_07460"
     CDS             1665..>1946
                     /locus_tag="PLW82_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOJ47148.1"
                     /translation="MNEILSQQELDALLSSVKETVTGQTKKIKRYDFKHPDKFSKDHM
                     RTLQMLHENLARLWSSSLSAHLRAPVHIELISLEQTSYEEFVSNLPNPTL"
BASE COUNT          525 a          455 c          498 g          468 t
ORIGIN      
        1 ccagatgggc cagaacatct ttaagttcgt ggccaacatg gctccggctt ttggtatgct
       61 cggtacgttg atcggtttaa ttcagatgct taccaccatc gacaacccag atgctatcgc
      121 tgcagccatg ggtgttgcgc tgattaccac cttctacggc tcacttcttg tcaacgtggt
      181 tgctactccg ctcgcaggaa agcttgcaat cagatcgaga aatgaggtat tgttgaagga
      241 actcatggta gaaggcattc tgtctctgca agctggtgag aacccacgca tagtggaaga
      301 gaagctgaaa tcgttcctct caccgcaaag ccgcaacgga ctccaggagt tcagaagaga
      361 tagggaacag gtgatgcgca gtgcgtcgta gaaaaaaacc tcaggcagaa gaaggtggcg
      421 gagcacccag ctatatgact acttggggtg acatgatgag cttgcttctc tgctttttca
      481 tcctgcttta cagcttttca tcccttgata accaacggtt tagagaaatt atggtggctt
      541 tcaagaacat ggcagggttt ttggatggag gaaccaacct tatacctgaa ggctacaact
      601 ccgaggagct ttcaccgttt gcagactcaa gccagattca gcttcaaagc ttagaggctc
      661 ttgctgctgt aatccaagat cttttagaag aagaaggatt tgtggatcag gtagaactct
      721 ccctagaaga tcgcggtttg gtgatgcgtt ttaaggatgt ggttctcttt gatataggtt
      781 cggatgcact gaaagaagca ccaaaggaga tgctcaaaaa actcagcgtc atcctggaaa
      841 aagttcccct gcccattcgc atcgagggcc atacagacaa cgtccccatc aataacgcga
      901 gattcccttc caactgggaa ctctcggtag ctagagcagt taaagttgtg cgcttcttga
      961 tcgaagaggg ttcgctggac tccgaaaggc tttcggcgtt gggttacggc gagtaccgtc
     1021 ccatcgaatc caacgctact cccgaaggta ggaacaggaa ccgcagggtt gatattatag
     1081 ttttgcatcc gtcgctagca ggaagcgaac ccggtgccga atggcttgaa gagttaagta
     1141 ggacttaccc agttaagtaa ggcaagagag gatgaattgg atggctgaaa aaggcaagat
     1201 tgtcgtagat gccaaattcg tggtgttttt aatagctttg gtaattctgg ctacatcagg
     1261 tagcgctctg cttactagtc gcctacttgc ccagcctaaa gtggtcgaag cggtggagga
     1321 caaacctgag ttcggtcccc gcgtggagtt tgacgccatt accgtcaact tacaaaacgg
     1381 tgtactcggt caaaaagctc cgcttatccg cgtgaagctg gtcgtggagc tcggcaccga
     1441 aaaaatcccc gacgactttg acgtgtacaa gcccgttttg caggataagg taatcgagct
     1501 tttacgcagc aagacagcaa cagatctaac aggcggagaa ggtcagatta gactcagaca
     1561 ggagatccga aaggcattca acgagatact gccgagagaa cctgttacca gcgtttactt
     1621 tactgaattc ttgattggca attagagata aggtggtgag ctaaatgaac gagatactgt
     1681 cgcagcagga acttgacgcg ttactttcca gcgtgaaaga gaccgtcact ggtcagacga
     1741 agaagatcaa acgctatgat ttcaagcatc ctgacaagtt ctccaaagac catatgagaa
     1801 cgctgcagat gctccacgag aacctggcaa gactgtggtc gtcatcgttg tcagctcacc
     1861 tcagggcccc ggtccacatc gaacttatct ctcttgaaca gacaagttat gaggagtttg
     1921 tcagcaacct ccccaatccc acactc
//
LOCUS       DAOSDA010000333         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_39080_length_1942_cov_2.983572, whole genome shotgun sequence.
ACCESSION   DAOSDA010000333 DAOSDA010000000
VERSION     DAOSDA010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39080_length_1942_cov_2.983572"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /gene="trmFO"
                     /locus_tag="PLW82_07465"
     CDS             complement(<1..724)
                     /gene="trmFO"
                     /locus_tag="PLW82_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOJ47149.1"
                     /translation="MVVKVIGGGLAGSEAAWQLAKRGIEVELYEMRPVASTPAHTTEN
                     FAELVCSNSLGSNEITSAQGLLKEELRRLGSLLIAMAEKARVPAGGALAVDREVFSKL
                     VTEEIEKNPLITVIRQEVTEIPDGNVIIATGPLTSDKLAEELARFVGDEHLYFYDAAA
                     PIVTRESIIEEKGFWASRYDKGEGEYFNAPLDKDEYERFYNELISAEVANKHAFEEDL
                     FFEGCMPIEKLALRGFDTLRYGP"
     gene            complement(757..1656)
                     /locus_tag="PLW82_07470"
     CDS             complement(757..1656)
                     /locus_tag="PLW82_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47150.1"
                     /translation="MGGSGVAFLDDAGAMRLNPAALNQINAVSVLAEGAASFDELSNL
                     VNVLSSRSALAKLKDGYESRVEGFLGFSTNRFGLGLATLVTVSKDQNKAEVYTFRTSN
                     VALALPFGILWKAFDNTYIGANIKLHHPVLEEFDLSGEKMQKKITRGSGQGFDLGLLV
                     DFGSIKLGASASDLSAFISMDGESQPEDIAPRFAVGASVDFFHKKRPGLIALAIDYTD
                     VYLPQGVQNGILRVGMENILFKTLTIRSGITAKDIFHDARVAQYSAGLGVKLGPVVLD
                     FAVVSSDLSTENMEGSLSFEIRL"
BASE COUNT          479 a          520 c          412 g          531 t
ORIGIN      
        1 gagggccgta tcttaaggtg tcaaaccccc gcagagccag cttttcaatg ggcatgcatc
       61 cttcgaaaaa cagatcctcc tcgaatgcgt gcttgttggc aacttctgca ctgatcagtt
      121 cgttgtagaa cctttcgtac tcgtctttat ccagcggggc attgaagtac tctccctcgc
      181 ctttgtcgta ccttgaagcc caaaaaccct tttcttcgat gatcgactct ctggtgacta
      241 ttggtgctgc cgcatcatag aagtaaaggt gctcatcacc aacaaaccga gcaagctctt
      301 ctgccagttt gtcggaggtg aggggtcctg tagcgataat gacgttgccg tcaggaatct
      361 cggtaacctc ttggcgaatc actgtgatca gtgggttttt ctcaatctct tcggtgacga
      421 gcttgctgaa aacctctcga tctactgcaa gagctccccc cgcagggact cttgcctttt
      481 ctgccatggc aataagcaga gagccgagtc gcctcaactc ctctttgaga agcccctggg
      541 cagaagttat ctcgttagaa cctaagctgt tggaacagac caattctgca aagttttccg
      601 ttgtatgggc aggagtactg gctacaggtc tcatttcgta gagctctacc tctatcccgc
      661 gttttgccag ctgccatgca gcctcgctcc cggcaagccc tccgccaatg actttaacaa
      721 ccattacata acctcatgaa agcggtctgt tttctcctac agcctaatct caaaactgag
      781 gcttccttcc atgttctcag tggaaaggtc agaagacacc accgcaaaat ccagcactac
      841 cggtcctagc tttacaccaa gacctgcaga gtactgagct actctagcat cgtggaaaat
      901 gtccttggct gtgatgcctg aacggatcgt caaggtttta aacaaaatgt tttccatgcc
      961 cactctaaga attccattct gcactccctg gggcaggtac acatcggtat agtctattgc
     1021 taatgcaatg aggccaggcc gctttttgtg gaaaaagtcc acgctcgctc caacagcaaa
     1081 gcggggagcg atgtcttcgg gctggctctc cccgtccatg gatatgaacg ccgagaggtc
     1141 gcttgcactt gcgcccagct tgatgctgcc aaaatccaca agcaatccta ggtcaaatcc
     1201 ctgaccgctg cctcttgtaa tcttcttctg catcttttct cccgacagat cgaactcctc
     1261 aagcactggg tgatgcagtt tgatgttcgc gccaatgtaa gtgttgtcga aggctttcca
     1321 aagaattccg aatggaagtg ctaaggcaac attggaggtg cggaaagtgt aaacctctgc
     1381 tttattttga tccttgctta ctgtaacaag tgttgccagt cccaaaccaa acctattggt
     1441 gctaaagcca agaaaccctt cgactcggct ctcatatccg tcttttagct tcgctaaagc
     1501 agatcgtgac gagagaacgt tcactagatt gctgagttca tcaaagcttg cggccccttc
     1561 tgctaatact gaaacggcat ttatctgatt tagcgcagct ggattgaggc gcattgcacc
     1621 tgcatcgtcc agaaacgcaa cgcccgaacc acccatcgct atggattttg tggttggttt
     1681 ataggaggta tcaaaagggg agcttgctaa aacgcatgct gataccacaa caaccaatac
     1741 tggtaaaatt aggaaacgta gctttttcat atcaccataa actctcctct ttttctatcc
     1801 acatttaaaa ctgctgattt acctcttatt cacagactta tccacattat ccacaagcag
     1861 ttattcacac actgtgggta actattacca aattctacct atatcatgct tctattctac
     1921 acttttatgc acaggtttgc ta
//
LOCUS       DAOSDA010000334         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_39511_length_1929_cov_3.075774, whole genome shotgun sequence.
ACCESSION   DAOSDA010000334 DAOSDA010000000
VERSION     DAOSDA010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39511_length_1929_cov_3.075774"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..710)
                     /locus_tag="PLW82_07475"
     CDS             complement(18..710)
                     /locus_tag="PLW82_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ47151.1"
                     /translation="MKKYLLVFVLFAIVMTSSAFAALPPTDVQKTHWAYESIMKILDS
                     GLMEGYPDGSFRGQQTIDRYEMAVFVARLLDVLAGIQADYEQKVGGIEGRVAELEKRP
                     SAVQSDNQMIKEILGLIMELEAEFENDLLEINNRYYDLQLDYGMLEDALQSLESTQTS
                     LSRRLDVYQVQIDSLNQKYADVTSTRDEVDALRKEVTELNQQLEDQSKTIRSLFIAIG
                     VMGAVVLLVSLN"
     gene            complement(715..>1929)
                     /locus_tag="PLW82_07480"
     CDS             complement(715..>1929)
                     /locus_tag="PLW82_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HOJ47152.1"
                     /translation="LKEKIGGKPIYAFDTIQRLAITTSDARSSQYYSATHEWAILKDE
                     VENLGQTDKQAKLDQLESFIPEDIRNDYLAARARNHVINSLAIDLVQEGVIDYLILAQ
                     DDASLTGLHRAEREVLKAKVDELGLEDKVMIFPGADEVGVILVSRAVTHHYGLEPTFK
                     VFYSGIEGSRWVAPFEDVNFAANIARHLEAVGARIAENGLIDLFVVTPGGNDSFSAGK
                     VKDLMEQGRRVVICDVVVTNSAHPGFFEAMTDQVEVAKLTGFAGWNTAGNTLGLAIGQ
                     AVSSLARDKLEGEELENATKAHFEYLLHRLAKDYFYKVQVQGEIERFTRSNGIDTINI
                     PENLFGFVQEELGFLLRPRLNDFYRKHFAGRSVGDYRLGDAVDWDIKLAWPRFFEIQI
                     IPEISLAIVREE"
BASE COUNT          439 a          529 c          437 g          524 t
ORIGIN      
        1 ttgtatcctg agacttctta gttcaagctt acaaggagta ccactgcacc cataactccg
       61 atagcaataa acaagcttct gatggtcttg gattgatctt cgagctgctg attcagttcg
      121 gtaacctctt tacgcagcgc atccacttca tctctggtgc tggtcacgtc ggcatacttc
      181 tggttcaggc tatcaatctg cacctgatag acgtccagac gccgagagag actggtctgt
      241 gtagactcga ggctctgcag ggcatcttct aacattccgt agtccagctg aagatcgtag
      301 tagcggttgt tgatctctaa aagatcattc tcgaattccg cctcaagttc cataatcaag
      361 cccaaaattt ccttgatcat ctggttgtcc gactggactg cagacggtct tttctcgagt
      421 tctgcaactc taccctctat gccgccaacc ttttgctcgt aatcggcttg aatgccggca
      481 agcacgtcca aaagccttgc cacaaacacc gccatctcat acctgtctat ggtctgctgc
      541 ccgcgaaacg atccgtctgg atagccttcc atcaagccac tatccaaaat cttcataatg
      601 ctttcatatg cccagtgggt tttttgcacg tctgttggag gtaacgctgc aaaggctgaa
      661 ctggtcataa ctattgcgaa gagaacaaaa actagtaggt attttttcat agcctcactc
      721 ctctctaaca attgctaaac tgatctccgg tattatctga atctcgaaaa acctcggcca
      781 tgccagtttg atatcccaat cgacagcatc accgagtcgg tagtctccca cacttctgcc
      841 ggcaaaatgc tttctgtaaa agtcgtttaa gcggggtctg agcaaaaatc ccagttcctc
      901 ctggacaaac ccgaagaggt tttcggggat gttgatggtg tcgataccgt tgcttctggt
      961 aaacctctcg atttctccct gcacttggac tttgtaaaag tagtcttttg ccaggcggtg
     1021 aaggaggtac tcaaagtggg ctttagttgc attttccagc tcttctcctt ccagcttgtc
     1081 cctagccaga gaggagaccg cctgtcctat ggccagtccc agcgtgtttc cggcagtatt
     1141 ccaaccggca aaccctgtaa gcttggccac ttccacctga tcggtcatgg cttcaaaaaa
     1201 ccctgggtga gcggaattgg tgacgaccac atcgcagatg acaactctcc tgccctgctc
     1261 catcaggtct ttaacctttc cggcagaaaa gctgtcgttt cctcctggag ttacaacaaa
     1321 caaatcaata agtccgtttt cagcgattcg ggcacctaca gcctctaagt gccttgcgat
     1381 gtttgcagca aagttgacgt cttcaaaagg ggcgacccag cgggaaccct caattcctga
     1441 gtaaaacacc ttgaacgtgg gctccaaacc gtagtggtgg gtcactgctc tgctgaccaa
     1501 tatcacaccc acctcgtctg ctccgggaaa gatcattact ttatcctcaa gcccgagctc
     1561 gtctaccttt gccttgagca cttcccgttc agcgcggtgc aggcctgtca gcgaagcatc
     1621 gtcctgggcg agaatgaggt agtcaataac tccctcttga acaaggtcga tggccaggct
     1681 gttaatcacg tggtttctcg ctctggctgc taggtagtcg ttcctgatgt cttcgggaat
     1741 gaagctctcc aactggtcca gcttagcctg tttgtccgtc tgaccgagat tttccacctc
     1801 gtccttgaga atcgcccact catgtgtagc actgtagtac tgggacgaac gagcgtcgct
     1861 ggttgtgatg gcaagccgct ggatggtatc aaaagcgtag atcggtttgc ctccgatctt
     1921 ctctttgag
//
LOCUS       DAOSDA010000335         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_39582_length_1927_cov_2.272970, whole genome shotgun sequence.
ACCESSION   DAOSDA010000335 DAOSDA010000000
VERSION     DAOSDA010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39582_length_1927_cov_2.272970"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /gene="cgtA"
                     /locus_tag="PLW82_07485"
     CDS             <1..624
                     /gene="cgtA"
                     /locus_tag="PLW82_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HOJ47153.1"
                     /translation="ADIPGLVEGAAEGVGLGHAFLRHVERCRVICHLVDASGSDGRDP
                     VEDVKVIRSELQKFSQELSERPEILVATKIDLGCEENLRRLEDAYGKVYPISAVTHEG
                     IDRLLQAILDLLKDLPKKPLEIDRVDIEFSPDAEDDYTIEETEDGYVIYGKGVEWISR
                     FDIRNYEAMQYIETRLEHMGIMDRLRELGVEDGDLIRIGELEIEYLS"
     gene            646..1767
                     /gene="proB"
                     /locus_tag="PLW82_07490"
     CDS             646..1767
                     /gene="proB"
                     /locus_tag="PLW82_07490"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOJ47154.1"
                     /translation="MNAFRDVKRIVIKVGTSSLTYENGNLNLGAFEVLVRAISDLSNN
                     GVQVVLVSSGAIAAGVNRLGWAKKPKAIPLKQAAAATGQPLLMHIYEKLFAEYGKTAA
                     QILLTRADLAVRNRYVNAQNTMETLLNENVIPIVNENDTVVVDELKFGDNDTLSAQVA
                     VLCEADLLVIFSDVEGLYTKDPRVDQTAELISEVPVLTEEHWQNARGPGSRRGTGGMY
                     TKLLAADIATKGGIATIIAHSKHQPRLKEIIQGAPIGTFFHPHKSALKGKSRWVMFGA
                     ASSGSIVVDNGAKEALLEQGKSLLSNGILEVKGDFKRGDVVVVEDEDGSIIARGQSRF
                     SSDELLEMIGLCSDELKTLGKNSNVAIHRNDLVVLRRDD"
BASE COUNT          522 a          452 c          555 g          398 t
ORIGIN      
        1 gccgacattc ccggtcttgt ggaaggtgct gccgagggcg tagggctggg tcacgctttt
       61 ctccgccacg ttgagcggtg cagggtgatc tgccacctgg tagacgcgtc tggcagcgac
      121 ggacgggacc cggtggagga cgtgaaggtg atcaggagcg aactgcagaa attcagccaa
      181 gagctgtcag aacgccctga aattctggta gcaacaaaga tcgatctggg ctgtgaagag
      241 aacctcagga gattggaaga cgcttacggg aaagtctatc ctatctctgc agtcactcac
      301 gaaggtatcg ataggctctt acaagccatt ttggaccttt tgaaggatct tcccaagaag
      361 ccccttgaga tcgatagagt tgatattgag ttttctcccg atgcagaaga cgactacacc
      421 atcgaagaga ctgaagacgg ttacgtcatc tatggcaagg gtgtggagtg gatcagccgc
      481 ttcgacatcc gtaactacga agctatgcag tatatcgaaa cccggcttga gcacatggga
      541 attatggata ggctgagaga gctgggcgta gaagatggtg acctcatccg catcggggag
      601 ttggagatag aatatctgtc atgattggag gaagtgccgt gagaggtgaa tgccttccgc
      661 gacgttaagc ggatcgtaat caaagtcggt acaagcagct taacctatga aaatggcaac
      721 ctgaacctag gtgcttttga agtgctggtc agggctatta gcgacttaag caacaatgga
      781 gttcaggtgg ttttggtttc gtccggagcc atcgccgctg gggtgaacag gctgggttgg
      841 gctaaaaagc ccaaggccat accccttaag caggcagcag cagcgacggg ccagccactt
      901 ttgatgcaca tctacgaaaa gctctttgcc gagtacggca aaacagctgc ccaaattctc
      961 cttaccagag cggatttggc agtgagaaac cggtatgtaa acgcccaaaa caccatggaa
     1021 acactgctga acgaaaacgt cattcccatc gtcaatgaaa acgacaccgt ggtggtcgat
     1081 gagctgaagt tcggcgataa cgacactctg tcggcgcagg ttgcagtcct gtgcgaagca
     1141 gacctcttgg taatcttttc cgatgtggaa ggtctctaca ccaaagaccc cagggtagat
     1201 caaacagctg agctcataag cgaggtgccg gttcttaccg aagagcactg gcaaaacgcc
     1261 agaggtccag gatcccgccg gggaaccgga gggatgtaca ccaagcttct cgccgcagac
     1321 attgccacca aaggcggaat cgcaaccatc atcgcccact ccaagcacca gccgaggctc
     1381 aaggagatca ttcagggagc gccaattggc acctttttcc atccccacaa gagcgctctc
     1441 aaagggaaga gccgctgggt gatgttcggt gcggcctcaa gcggtagcat cgttgtggac
     1501 aatggggcaa aagaggccct tttagaacag ggaaagagtc tcctttccaa cggcatcttg
     1561 gaagtgaagg gagatttcaa aagaggcgac gtagttgtcg tcgaggacga agacggatcc
     1621 ataatagcca gaggacagtc gcggttcagt tccgacgagc ttttggagat gatcggtctt
     1681 tgctctgatg aactgaagac cttggggaag aatagcaatg tggctattca ccgcaacgat
     1741 ctggtagttt taaggagaga tgactagtgt ctgagcttca cttgaaaagc aggctggcca
     1801 aagagtcgac aagaatactg gccaacatgt catctgcaaa aaaacgcgaa gccctactga
     1861 agatggcaga cggcttagag cagtcccacg gtgtgatttt gaaagctaac aagaaggaca
     1921 tggaaga
//
LOCUS       DAOSDA010000336         1924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_39670_length_1924_cov_3.090423, whole genome shotgun sequence.
ACCESSION   DAOSDA010000336 DAOSDA010000000
VERSION     DAOSDA010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39670_length_1924_cov_3.090423"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PLW82_07495"
     CDS             complement(<1..812)
                     /locus_tag="PLW82_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47155.1"
                     /translation="MNKKNITRLIVVLLLLMSVALTGCAAYKVVSEESKTEEIARGVT
                     LTEKRIQTDSGWVDIFILEADLSVEGVKVDAVYSSEGLGKTQTMSKMTKDSGAIAAVN
                     ADFFLISSTGAPIGGQMSDGRLIKTPAPGLTGWTHFAIDIENIADLITPVFLTNVIFE
                     DGTRKNIEAFNCEVDFSWTRTVVYDANFYKTTPGANSIMIKNAWDYTEVVVDGENKIV
                     DVRTKQPAVPIPEGGFVIAGSNQDAEFLRSVAKVGETVKFNVYADPSLDSLQS"
     gene            complement(1139..>1623)
                     /locus_tag="PLW82_07500"
     CDS             complement(1139..>1623)
                     /locus_tag="PLW82_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOJ47156.1"
                     /translation="QPIPTPEPKPQVPEPKPQVPEPKPTPSTPSAPAGLSASEDWMFN
                     KINEERAKAGVRPLIRDPELDRIARIKSQDLVDNNYFAHESPTYGSVSNMLRSFSYSF
                     RGAGENLAKSQSAEISHYRLMASDGHRSNILYSAYTHVGIGVVRYKSGVMVTQIFAIK
                     "
     assembly_gap    1624..1633
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1634..1815)
                     /locus_tag="PLW82_07505"
     CDS             complement(<1634..1815)
                     /locus_tag="PLW82_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47157.1"
                     /translation="MRQLLNRFGISVLVAVTVLVLYAMPASAATYNWSGYQVKVPRYL
                     NSYFDKIFGTSPSETPA"
BASE COUNT          470 a          457 c          454 g          533 t
ORIGIN      
        1 gactgcaggc tatccagaga aggatctgca tagacgttga acttcactgt ctctccaact
       61 ttggccaccg agcggagaaa ctccgcatcc tggttgctgc ctgcaatgac aaatccaccc
      121 tctggaatag gcactgctgg ttgtttggtt cttacatcaa cgattttgtt ttcgccatcg
      181 actacaactt ctgtgtaatc ccaggcgttt ttgatcatga tgctgtttgc gccaggcgtt
      241 gttttgtaga agttagcatc ataaactacg gttctggtcc aagaaaagtc cacttcacag
      301 ttgaatgcct cgatgttttt cctggtaccg tcttcaaaaa tcacgttggt aaggaaaacc
      361 ggtgtgatca gatctgcgat gttttcaata tcgatggcaa agtgagtcca tccggtcaaa
      421 cccggagctg gagtcttgat caacctgcca tcggacattt gacctccaat aggtgctcct
      481 gtactggata tcaggaagaa gtctgcattt accgcagcga ttgcaccaga gtctttggtc
      541 attttgctca tggtctgggt tttgcccagt ccctcgctgc tgtaaaccgc atcaactttg
      601 actccttcaa cgctgagatc ggcttcgaga atgaagatat cgacccagcc cgaatcggtt
      661 tgaatacgct tttcggtgag agtcacgccg cgagcgatct cttcggtctt agactcttcg
      721 gagacgacct tgtacgcagc acaaccggtg agtgcaactg acatcaagag cagaagaaca
      781 acaattaacc gtgttatgtt ctttttgttc actaaaccct tcctcccccg taaagtaact
      841 gcaaatttct gtttataact ttcccttagt gcaagaaatt tcctgcataa catgatcttt
      901 aacgaatagc aatcttcctg tgctttccct gaaggcttgc ctacaagtaa gcaccggcga
      961 agtttcaggt ctgttttgta aaaagcgtgc acttcaaggt gcatagcatc tgtcatctga
     1021 gcttgcacct ggtggtttga aaacagcagg cagccgaagt atgtactcag cgtaaaaaaa
     1081 gaaagggctg ctagaagacg accgttctgg tcacttcctg ctgccctttt ctttacggct
     1141 acttaattgc aaagatctga gtcaccatga ctccggattt gtagcgtacg acgccgatac
     1201 ccacgtgggt gtatgcagaa tagagaatgt tcgagcggtg tccgtcgctt gccataaggc
     1261 ggtagtgaga gatctctgcg ctctggctct ttgccaggtt ctcacctgct cctctgaagc
     1321 tgtagctaaa ggatctcagc atgttagata cgctaccgta ggttggcgac tcgtgagcga
     1381 agtagttgtt atctacaaga tcctgactct tgatcctcgc gatgcggtca agctctggat
     1441 cgcggatcaa aggtcttacg cccgctttgg cacgctcttc gttaatctta ttgaacatcc
     1501 aatcctcaga tgcagaaagc cctgcaggag cggaaggtgt gcttggagtt ggcttaggtt
     1561 caggaacctg tggcttaggt tctggcacct gcggctttgg ctctggagtt ggtataggtt
     1621 gttnnnnnnn nnngcaggag tctcgcttgg gcttgtccca aaaatcttgt caaaatagct
     1681 gtttaaatag cggggaacct ttacctgata accagaccag ttgtaggtgg cagctgaagc
     1741 aggcatcgca tacagaacca atacagttac agcaacaagg actgaaatac caaatctatt
     1801 caaaagttgt ctcatcttct ctcctccttt gaacgatctg attatacagg catagagtac
     1861 tacagggcaa aaggggtgaa cagaggtcat tctgcttgaa aacaaattac tcttggtatg
     1921 gtct
//
LOCUS       DAOSDA010000337         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_39785_length_1920_cov_3.910992, whole genome shotgun sequence.
ACCESSION   DAOSDA010000337 DAOSDA010000000
VERSION     DAOSDA010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39785_length_1920_cov_3.910992"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PLW82_07510"
     CDS             complement(<1..1016)
                     /locus_tag="PLW82_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOJ47158.1"
                     /translation="MRKFVINLVKPYWKRYVIGISALLIVNFTQSLIPLLLQNTINAV
                     SEGLLTYAGIAKQAVLILGFALFMAVGRWAWRIYVMGTSRLIECDLREKLYLHLQSLS
                     QNFYNHHKTGDLMAHATNDVIAVRQALGNGVLMAFDAVSLIFMSFFLMFRTHTQLTIY
                     SLIPLPLMIIASIYLSKLIGTRFRRVQEQFSNLTDKVQENISGMRVVKAFVQENAEIE
                     RFAEVNKQNFDAQMSMVKIANLMRPLSALVSSLSFIVVLWFGGRLVIDGILQIGDLVG
                     FSMYLGNLAWPLMSIGWLFNLVNRSRVSMQRLWELFCAEPEIKDGFMTNPNLTSLSGE
                     IEFR"
     gene            complement(1274..>1920)
                     /locus_tag="PLW82_07515"
     CDS             complement(1274..>1920)
                     /locus_tag="PLW82_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HOJ47159.1"
                     /translation="KITVPVALHLDHGSYEAALACIDAGFSSVMFDGSHYPIEENITK
                     TKELVRITGEKGISLEAEVGSIGGEEDGVVGTGEFADPAECEMMKDLGVTMLAAGIGN
                     IHGKYPANWPGLSFETLEAISKRVGDLPLVLHGGTGIPEDQIKKAISLGVSKINVNTE
                     CQLAFAEATRKYIEAGKDLEGKGFDPRKLLAPGTDAIKATVREKMELFGSVNKA"
BASE COUNT          478 a          480 c          451 g          511 t
ORIGIN      
        1 tttctgaact cgatctcgcc gctaagagag gtgagatttg ggttggtcat gaagccgtct
       61 ttgatctccg gctcggcaca gaagagttcc cacaggcgct gcatcgaaac ccttgaacgg
      121 ttgaccaagt tgaaaagcca gccgatactc atgagaggcc aggctagatt gcctaagtac
      181 atggaaaagc ccacaagatc gccgatttgg agaatgccat cgatgaccag cctcccgcca
      241 aaccagagaa ccacaataaa agacaaggag ctaaccagtg cgctcaatgg cctcatgagg
      301 ttggcgatct tcaccatgga catctgagcg tcgaagtttt gcttgtttac ttctgcgaac
      361 cgttcgattt cggcgttctc ctggacaaac gctttaacaa cccgcatgcc ggagatgttt
      421 tcctgaacct tgtcggtcag gttgctgaac tgctcctgca cgcgtctgaa cctggtaccg
      481 atcagtttgg agagatatat ggatgcgatg atcatgagcg gcagtgggat aagagagtag
      541 atggtcaact gcgtgtgggt cctgaacatt aagaagaaac tcataaaaat caatgagacc
      601 gcatcaaaag ccatgaggac gccatttccc agagcttgtc ttacggcgat tacgtcgttt
      661 gtggcatggg ccattagatc ccctgttttg tgatggttgt aaaagttctg cgacagactc
      721 tggagatgga gatacagctt ctccctaagg tcacattcga tgagcctgct cgtacccatt
      781 acatagatcc gccaagccca gcggcctaca gccatgaaca aagcaaaacc caagatcaaa
      841 accgcttgct tggcgatgcc agcataggtt aggagtcctt cagatacagc gttgatggtg
      901 ttttgcagaa gaagcgggat taacgactgg gtaaaattaa ctatcaagag tgcagaaata
      961 ccaataacat agcgcttcca atacggttta acaagattaa taacgaattt tcgcacctgt
     1021 gcacctcctt caaatgttct atttctgcac agtttccttt gagcctgcag tgtttccgtg
     1081 aaatttgcaa atcccaaact tttggcccaa cttccggtcc tcggctcttg agccaagtac
     1141 aaattaaagt agccactttg cgcaatgcgt agcactgcat ttgctttccg cttgcatttt
     1201 tgcgtttcgc agatctgggc tatagacagg ttaagccctg catgctgcag ggcttaattg
     1261 ctggatattc agtttaagct ttatttacag atccgaagag ttccatcttc tcccttactg
     1321 tggccttgat ggcatctgtt cccggagcta gcagtttgcg aggatcaaag cctttgcctt
     1381 ccaggtcttt accggcttcg atgtacttcc ttgttgcttc ggcaaaagcc agctggcatt
     1441 cggtgttgac attaatcttc gaaacgccaa ggctaatagc cttcttaatt tggtcttcgg
     1501 gaattcccgt tccgccgtgg agcaccagag gcagatctcc tactctctta ctgatggctt
     1561 cgagagtttc aaacgacagt cccggccagt ttgcaggata ttttccgtga atgtttccga
     1621 tacctgcggc gagcatggtc acgcccaagt ccttcatcat ttcgcattct gctggatcgg
     1681 caaactctcc tgtaccaacg acaccgtctt cctcaccgcc gatagaaccc acttcggctt
     1741 ccaaggaaat gcctttctca cctgtgattc taaccagttc tttggttttg gttatgtttt
     1801 cctcaatggg atagtgagaa ccgtcgaaca tgaccgaaga aaaacctgca tcgatgcaag
     1861 ctagagcagc ttcataggat ccgtgatcta ggtgaagcgc tactggaact gtgatcttga
//
LOCUS       DAOSDA010000338         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_39981_length_1913_cov_3.578041, whole genome shotgun sequence.
ACCESSION   DAOSDA010000338 DAOSDA010000000
VERSION     DAOSDA010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39981_length_1913_cov_3.578041"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLW82_07520"
     CDS             complement(<1..301)
                     /locus_tag="PLW82_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ47160.1"
                     /translation="MKGKWDAILFDMDGTLIEIDIKEFLKAYFGLWLETAKKHTERPE
                     DFVNNLIASVDDMIKSTDPSLTNEEVFWQSFSRFGWDPEVYKPIAKGFYENQFQSL"
     gene            389..1162
                     /gene="pdaB"
                     /locus_tag="PLW82_07525"
     CDS             389..1162
                     /gene="pdaB"
                     /locus_tag="PLW82_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family sporulation
                     protein PdaB"
                     /protein_id="HOJ47161.1"
                     /translation="MFFVFRISRKRLMVVGLLALALLAGVVSGPLVRTTAGAARLIPV
                     YSIDTKEKVVALTFDISWGTLVPEPVLDVLREKEVTSTFFLSGPWVERNQEIALKIKE
                     DGHEIASHGYRHVNLSGLSAAEIQTELLKASRAIEDVLRVKPWLLRTPNGDYNNKVVE
                     AAESLGYTVIQWDTDSLDWTKPGVKAIVDRVLKKVHPGDIILFHASDSATDTPEALPL
                     VIDALRKEGYRFVTVSELLQLDPQKRWENMPGPPKRQGT"
     gene            complement(1301..>1913)
                     /locus_tag="PLW82_07530"
     CDS             complement(1301..>1913)
                     /locus_tag="PLW82_07530"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="catalyzes the formation of spermidine from
                     putrescine and S-adenosylmethioninamine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HOJ47162.1"
                     /translation="NPKRVCVIGGGDGGTVREVVKHPLVTEVKHVEIDGAVIEAAKKY
                     FPTLSSGLADPRVEIVIADGIEYIKNAEAGSFDVIIIDSTDPVGPAVGLFSAEFYGYC
                     HRALTDDGVICAQTESPLFEKDLLIKCWNNMKVHFSNVGVALGFAPSYPGGLWTYTVG
                     SKQLPVQKNREVQIEGRYFKNSIMEAAFDTPQFLQEVLASGQK"
BASE COUNT          480 a          515 c          430 g          488 t
ORIGIN      
        1 ctagggactg aaactggttt tcgtagaaac ctttagcgat gggtttgtag acttctggat
       61 cccaaccgaa acgggaaaac gactgccaga agacttcttc gttggtcaaa ctaggatcgg
      121 tgctttttat catatcatcc accgaagcga tcaggttgtt cacaaagtct tcaggtcttt
      181 ccgtatgttt tttcgccgtc tccagccaca ggccgaagta ggctttgaga aactctttaa
      241 tatcgatttc gatcagcgtt ccgtccatgt caaacagaat tgcgtcccat ttacctttca
      301 tcgaccaacc ctcctttttg caattttaga tacccggcgt catctaggca tatacattaa
      361 agaaatcatt tacttgggag gtgagccctt gttcttcgta tttcgcatct cgcgcaaacg
      421 tcttatggta gtaggtctcc tagctcttgc gcttcttgca ggcgttgtct caggtccact
      481 ggtcagaacc actgcaggtg cagcaaggct tattcccgtg tattccattg atacgaagga
      541 aaaggtcgtg gctctgacct ttgatatcag ctggggaaca ctggttcccg aacccgtcct
      601 cgatgttctc cgggaaaaag aggttacctc tactttcttt ctctccggac cttgggtcga
      661 aagaaaccag gaaatcgcct tgaagatcaa agaagatgga cacgaaatcg ccagccacgg
      721 atacaggcac gtcaatctca gcggactgtc ggcagcagag atccaaaccg agcttctcaa
      781 agcatccaga gcaattgaag acgtcctcag agtaaaaccg tggctcctca ggactccaaa
      841 cggcgactac aacaacaaag tggttgaagc agctgaaagc cttggctaca cagtgatcca
      901 atgggatacc gactctttag actggactaa accaggcgta aaagccattg tggatagggt
      961 tctcaagaag gtccatcctg gagacatcat tctctttcac gcttcagaca gcgccacaga
     1021 caccccagaa gcactgcctc tggtcatcga cgccctgcgg aaggaagggt atcgcttcgt
     1081 caccgtcagc gaacttctgc agctagaccc ccagaaacgc tgggagaaca tgccaggtcc
     1141 tcctaaacgc cagggaactt aacacgcttt ccaaaacagc ggagcaagaa atcgctccgc
     1201 tgtttcccgt ctttacaaca tctatcatta acgcgtatca ctttggggtt cgccgatcta
     1261 cactccaagc ctttctttag agccactttt gccgtatagt ctacttttgg ccgctagcta
     1321 aaacctcctg caaaaactgc ggtgtatcga aggccgcttc cataatgctg tttttgaagt
     1381 atctcccttc gatctggact tcacggttct tctgtaccgg cagctgtttg ctgcctacag
     1441 tgtaagtcca aagaccgccg ggataggaag gtgcaaatcc taacgccacg ccaacgttgg
     1501 aaaagtgcac tttcatgttg ttccagcact tgataagaag atctttttca aatagaggcg
     1561 attctgtttg agcgcagatc acgccgtcgt ctgtaagtgc cctgtggcag tatccgtaaa
     1621 actctgcaga aaaaaggcct acagcaggac ctacagggtc tgtagaatcg ataatgatta
     1681 catcaaagga accagcctct gcattcttga tgtactctat gccatcggca atgacgatct
     1741 caaccctagg atctgctagc cccgagctga gcgtcggaaa gtattttttc gccgcctcaa
     1801 taacggctcc atcaatctca acgtgcttga cctcagtgac caaggggtgc ttcaccactt
     1861 ctctgacggt tccgccgtct ccgccgccga ttacgcacac tctctttggg ttt
//
LOCUS       DAOSDA010000339         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_40238_length_1905_cov_4.075135, whole genome shotgun sequence.
ACCESSION   DAOSDA010000339 DAOSDA010000000
VERSION     DAOSDA010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40238_length_1905_cov_4.075135"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..462)
                     /locus_tag="PLW82_07535"
     CDS             complement(250..462)
                     /locus_tag="PLW82_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOJ47163.1"
                     /translation="MSKNRKQVPLRLPEELYNKIAAWAEEDFRSINGQIEYLLTLCVR
                     QKEKTGRYTPQSFSNEDTETTEKETP"
     gene            complement(474..1499)
                     /locus_tag="PLW82_07540"
     CDS             complement(474..1499)
                     /locus_tag="PLW82_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOJ47164.1"
                     /translation="MEEKILKAKPGLPFLILFILLYLLGIGSIIFGGIQLDKGHLWGI
                     PFLVVGILWMVLGIIPFLGLKIIHPNEALVLTLFGKYVGTLKDHGFFFVHPFCSAVNP
                     AAMTKLGQSHDVDTQGRGKQVIEVSTVGLSQSTIPSKKISLKVSTLSNAKQKINDALG
                     NPVEVGVAVIWRVVDTAKAVFAVDNYKEYLSLQADAAVRNIVRIYPYDVAPNVDTTGD
                     GIPDEGSLRGSSETVAKRIRDEIQKRVEVAGLEILEARITYLAYAPEIAAAMLQRQQA
                     SAIIDARTMIVEGAVSMVEMALHQLSERDIVELDPERKAAMVSNLLVVLCGNRDAQPV
                     VNSGSLY"
BASE COUNT          487 a          436 c          435 g          547 t
ORIGIN      
        1 aagggtcttt tctgcaaata ctcctctgtt ttcagaacat cttttttcat ttgaggggtt
       61 ctctacacga aaaaaatgtc cttctgagaa ccctgggatt attcaaaaat cgtaagcgat
      121 gcctgtccca aaaaaccaaa gcgagagaat tgctgctcaa gcagcgctac catgagaaac
      181 catgaaaaag cactgcgggc aaacagaagg ttacgttcag cccgcagtgc gcacttggac
      241 gacttgtttt caaggcgtct ctttttcggt tgtttcagta tcttcattgg aaaagctttg
      301 cggtgtgtag cgccctgttt tctctttttg gcggacacac aaggttagca ggtattcgat
      361 ctgaccgttg atggaacgaa agtcttcctc agcccaagcg gcaattttgt tgtacagttc
      421 ttcaggaagc cttaggggga cctgttttct gttcttgctc atcttaggtg gtgttagtac
      481 aagctgccgc tatttacaac tggctgagca tctcggtttc cgcagaggac gaccaggaga
      541 ttcgatacca ttgcagcttt cctttcagga tcgagttcaa cgatgtccct ctcactgagt
      601 tggtgaaggg ccatctctac catactgact gcgccttcca cgatcatggt tctggcatca
      661 ataatagcac ttgcctgttg acgctggagc attgctgcag cgatctccgg tgcataagcg
      721 aggtaggtga tcctggcttc gagaatctcc aaacctgcaa cttcaaccct tttctggatc
      781 tcgtcgcgaa ttctcttggc cacagtttcg ctggagccgc ggaggctgcc ttcgtctggg
      841 atgccatcac cagttgtgtc aacgttggga gcaacatcgt aagggtagat tcttactata
      901 tttctaactg ctgcatcggc ctggagcgaa aggtactctt tgtagttgtc gacagcgaac
      961 acagctttag cggtgtctac tacccgccaa atgactgcaa caccgacctc taccggattg
     1021 ccaagtgcat cgttgatttt ttgcttggcg ttgcttaacg ttgagacttt caaagagatc
     1081 tttttgctcg gtatagtgct ttgagaaaga cctacagtgg aaacctcaat tacctgcttc
     1141 cctcttcctt gggtgtctac atcatggctt tgaccgagct tagtcattgc tgctggattt
     1201 actgcagaac agaaagggtg tacgaaaaag aatccatggt ctttcagagt tccgacgtat
     1261 ttaccgaaaa gagtgaggac tagggcttcg ttggggtgga tgatcttcaa accgagaaaa
     1321 ggaatgattc ccaaaaccat ccacagaatt cctacgacaa gaaacggtat gccccagagg
     1381 tgacctttat cgagttggat gcctccgaag atgatagacc cgattcccag gagatacagt
     1441 aggataaaga ggatgagaaa cggcaaacct ggcttggctt tcaagatctt ttcttccatc
     1501 atcaataccc cctcaatctt aattgtcttc tgatatctta aaggtatcat tttgatatcg
     1561 taaaaacaat acctactaaa caaaaagtat gaaatctttg caagtttatg agaagggttt
     1621 atgaggtgaa gatggtctct attggcgcaa aagcctctca cgtgggcttt tatccacatg
     1681 agaggctttg aaaagtcaaa gcttaatagc atttcctgtg taatcaacag tcaacagagc
     1741 aatatgtcga ctccgcttgt tcacactaat tctcactagc cagactgctg tttgttcttt
     1801 aacccaagat gttccgtaaa cgatgatgct cccaataacc tccgcttgct ttgtttttgg
     1861 tagtataaga atggcgaaaa tggcagtcta attttccaga gttgg
//
LOCUS       DAOSDA010000340         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_40399_length_1900_cov_3.124661, whole genome shotgun sequence.
ACCESSION   DAOSDA010000340 DAOSDA010000000
VERSION     DAOSDA010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40399_length_1900_cov_3.124661"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PLW82_07545"
     CDS             complement(<1..143)
                     /locus_tag="PLW82_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RuvX/YqgF family protein"
                     /protein_id="HOJ47165.1"
                     /translation="MRFVGLDLGEKTIGVAVSDALLFSAQPVTVIKRRGDLNTDLGLL
                     KEAL"
     gene            complement(146..1090)
                     /locus_tag="PLW82_07550"
     CDS             complement(146..1090)
                     /locus_tag="PLW82_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ47166.1"
                     /translation="MEYRRLGNTGIIVSRLCFGSLTIGPLQANLSPKEGGEVIRYALE
                     QGVNFIDTAELYDNYEHIREGIKDFPDVVVASKSYAVDYDEMRRSVDAARRSLGRDTL
                     DIFLLHEQESALTLKGHRRALDYLVWAKSQGHVKAIGISTHAVAAVRSAALMPEIDVI
                     HPLINLTGVGILDGTRREMEEATELAASAGKGLYAMKALGGGSLIPRAKEAFAYALSL
                     PQFCSVAVGMRRKNEVDLNVKLFSELAPSDTEWDEVLGEPRRLHIDSWCIGCGACAAR
                     CGQQAITIIDGFAVCDHDKCVRCVYCASVCPEFAIKVV"
     gene            complement(1127..>1900)
                     /locus_tag="PLW82_07555"
     CDS             complement(1127..>1900)
                     /locus_tag="PLW82_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47167.1"
                     /translation="PGAEVVYVLDVKSSQTAGQALGFIEAIELGDQYNVKAGEITELV
                     ESYPDITVRNLNDITGGKSETRPTVVQDDLTKARQQVVELLNKETVKVGSGEYLLPGS
                     TERAEPVVVYDQKVGDFAPTVTASGTQKVSYRKITGADVQQKIENILPNLLPTAYNLV
                     SGSPRVKNVEFEKDAVKVSASFELYPTLDAEEIASAILGKDPEEAEILLGEIGRLTFD
                     GLKDEESLLPTRKQWLKVVLSDEYDADKTFVNISLNQSI"
BASE COUNT          436 a          528 c          433 g          503 t
ORIGIN      
        1 agggcttctt tcaagagccc aagatcagta tttagatcgc cgcgtctttt tattactgta
       61 accggttggg cggaaaaaag tagggcatcg gaaactgcca cacctatggt tttttccccc
      121 aaatccagac ctacaaacct cattcttaaa ccacctttat ggcaaactcc gggcaaaccg
      181 aagcgcagta gacgcagcgc acgcatttat cgtggtcgca cacggcaaat ccatcaatga
      241 tcgttatggc ctgctgtccg cagcgcgcag cacaagcacc gcaaccgata caccaggagt
      301 cgatatgaag cctacggggc tctccgagaa cctcgtccca ctctgtgtca gagggagcca
      361 gttcggagaa gagcttgacg tttagatcga cttcgttttt tctcctcatt cccaccgcaa
      421 cagaacagaa ctgcggaagg cttaaggcat aagcaaacgc ttctttagct ctgggtatca
      481 gagaacctcc tcctagtgct ttcatggcgt acaaaccctt accagcacta gctgcaagct
      541 ccgtagcctc ttccatctcc ctgcgggttc cgtcgagaat gcccactccc gttaggttga
      601 tcagtggatg gatcacgtcg atctcgggca taagggcagc ggatctcacc gctgcaactg
      661 catgggtaga aatgccgatg gccttaacgt gcccttggga ctttgcccag actaagtagt
      721 ccagagctct cctgtggcct ttcagcgtca aggccgattc ctgttcgtgg agaaggaaaa
      781 tgtccagagt gtcccgaccg aggctgcgtc ttgcagcatc taccgaccgc ctcatctcat
      841 cgtagtctac tgcatacgat ttggaggcga ctaccacatc ggggaaatct tttatccctt
      901 ccctaatgtg ttcgtagttg tcgtacagct ctgctgtatc aataaaattg accccttgct
      961 ccaacgcata cctaataact tctccccctt ctttgggaga aagatttgct tgcaaagggc
     1021 caattgttag agatccaaaa caaagcctag atacaattat tcccgtattg cctaatcttc
     1081 tgtattccaa tgccaatgca ccagataggt gcatacacca ccttagctat atgctttgat
     1141 ttaacgatat attaacgaag gtcttgtcag cgtcatactc gtcgctcagg accaccttca
     1201 gccactgctt tcgggtaggc agaaggcttt cttcgtcttt caatccgtca aaggtcaacc
     1261 tgccgatctc tcccaagagg atttcggcct cttccgggtc tttccccaag attgcagagg
     1321 caatttcttc tgcatccagg gtaggataga gctcgaaaga tgcactgact ttaaccgcat
     1381 ctttttcgaa ctcgacgttt ttaacccgtg gcgaaccgct cactaaatta taggcagtgg
     1441 gcaggagatt tggcaagata ttttcaatct tttgttgaac atccgcaccg gttattttgc
     1501 gataggagac cttctgggtg ccgcttgctg taactgtcgg tgcaaaatcc ccgacttttt
     1561 gatcgtagac cactaccggt tcagcacgct cggtgcttcc gggcaagagg tactcgccag
     1621 agccgacctt cacagtttct ttgttcagga gttccaccac ctgctgtctc gctttggtta
     1681 ggtcatcctg gactactgtg ggacgggtct cgctctttcc gccggtgata tcgtttaagt
     1741 tccgcacggt tatgtctggg tagctttcca caagctcggt gatttctcca gccttgacat
     1801 tgtactggtc tccgagctca atcgcttcaa taaagcctaa ggcttgtcca gctgtctggc
     1861 tcgacttgac atcgagcaca tacacgacct cagctccagg
//
LOCUS       DAOSDA010000341         1878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_41109_length_1878_cov_2.935820, whole genome shotgun sequence.
ACCESSION   DAOSDA010000341 DAOSDA010000000
VERSION     DAOSDA010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41109_length_1878_cov_2.935820"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLW82_07560"
     CDS             <1..740
                     /locus_tag="PLW82_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ47168.1"
                     /translation="IRKRSSLLLDDKIMFDFNDALTFVHQYQLDLSKLEHLVITHSHS
                     DHFNIFDLEFKLRWYTNPGNPPLNIYANEKALSRFDLTPEFASWDKSKMELLQFHTVK
                     RWQPFFIDQYKITPLAANHVTSYAGEETCIYVVEKDNKRFLYGLDSGFYPEETWEYLK
                     SHYFHLMIFDSTLGLLDIKERTNHMSLAENVEVVEILRKSGSIDENSVLISTHFSHNG
                     RVVYDLHEKTYKEHGLIMSYDGMEIVL"
     gene            complement(784..1584)
                     /locus_tag="PLW82_07565"
     CDS             complement(784..1584)
                     /locus_tag="PLW82_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ47169.1"
                     /translation="MINLPLFKSTIKHNYKVFIIILAVLVMYFVIITQMFEPGAYESI
                     ETLLQALPSQLIDAMGLNVLEPSLLGYLAGYYYGFLIVVFPLLYSIIVANRLIAKHVD
                     RGSMAYLLATPNTRIKICLTQALFLVLSVTLLIASLAAIGILTCQALFPGELDISRFL
                     FLNFGALLLHWMLSGIGFLASCLFNDTKNSLGFGAGIPIFFVIVQMLMNASDKLKGLK
                     YVTLLSLFDPEGIISGGRVWPAFFAMFVFAAVFYGAAVYIFNSKDLPI"
     gene            complement(1562..>1878)
                     /locus_tag="PLW82_07570"
     CDS             complement(1562..>1878)
                     /locus_tag="PLW82_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ47170.1"
                     /translation="AGIIRQGRLVAVENIQTLKAERRKIYQITFGSEEQAKSFAGDYG
                     DIVEVNGVEVSAAVSGDVDKLIKILAQFEVVGLDVANLSLEDVFMHYYKRGGTDDQPS
                     AF"
BASE COUNT          540 a          471 c          443 g          424 t
ORIGIN      
        1 acatcagaaa gcgctcgagc ctgcttttag acgacaaaat catgttcgac ttcaacgatg
       61 cgctgacttt tgtccaccag taccaactag atctgtccaa gctggaacac ctggtaatca
      121 cccactccca cagcgatcac ttcaacatct tcgatctgga gttcaagctg aggtggtaca
      181 ccaatccggg aaatcctccc ctgaacatct acgccaatga aaaggccctt tccagattcg
      241 acctgacacc agagtttgcc agctgggaca agagtaaaat ggagcttttg cagttccaca
      301 ctgtaaaaag atggcaacct ttcttcatcg accaatacaa gataacccct cttgccgcaa
      361 atcacgttac atcatacgca ggcgaagaaa cctgcatcta tgtagtggaa aaggacaaca
      421 agagatttct ctacggcctg gactcgggct tttatccgga agaaacctgg gagtacctaa
      481 aatcccatta cttccacctt atgatcttcg actccacact aggcctttta gatatcaaag
      541 aaagaaccaa tcacatgtcc cttgcagaaa acgttgaggt tgtggaaatc ctgaggaaaa
      601 gcggttctat agatgaaaac tcggtgctga tttccaccca cttttcccat aacggcaggg
      661 tggtctacga cctccacgaa aaaacctaca aagagcacgg cctaataatg agctacgacg
      721 gcatggagat cgtgctgtaa cctgcatata ccactgcacc cttgaactat caagggtgca
      781 tttttatatc ggaaggtcct tactgttgaa gatgtagact gcggctccat aaaagaccgc
      841 tgcaaagaca aacatggcaa aaaacgcagg ccacactcgt ccccctgaga tgatcccttc
      901 gggatcaaac agagaaagca aagtgacgta ttttaggccc ttgagcttgt cgctggcgtt
      961 catcagcatc tgaacgatga caaaaaagat gggaatgcct gcgccaaagc ccagagagtt
     1021 cttggtgtcg ttgaagaggc atgatgctaa aaaaccgatg ccgctcagca tccaatgcag
     1081 aagcagcgcg ccgaagttca ggaacagaaa acgggatatg tccagttcgc caggaaagag
     1141 cgcttggcag gtgagaatgc cgatcgccgc aagggaagcg attaacaggg tcacgctgag
     1201 aacaagaaac agcgcttggg tcaggcagat cttgatcctg gtattgggcg tagccaggag
     1261 gtaagccata gaacctcggt ctacgtgctt ggcaatgagc cgatttgcaa caatgatgga
     1321 gtataacaga ggaaacacaa caatgagaaa gccgtagtag tatccggcaa gatagcctag
     1381 gaggctcggc tcgagcacat tcagtcccat agcgtctata agctgagagg gcaaagcctg
     1441 cagcagcgtt tcgatgcttt cgtatgcgcc tggttcgaac atctgggtga tgatgacaaa
     1501 atacatgaca agaacagcta ggatgatgat gaataccttg tagttgtgtt tgatggtcga
     1561 cttaaaaagc ggaaggttga tcatcagtac cacccctttt gtagtagtgc atgaagacgt
     1621 cttccaggct gagattggca acatcaagac ctaccacttc gaactgggcc aagatcttaa
     1681 tgagtttgtc tacatcgccg ctgaccgctg cactcacctc aacgccatta acctccacga
     1741 tatctccgta gtcaccggcg aaactttttg cctgttcctc ggagccaaag gtaatctggt
     1801 agattttccg ccgctcggct tttagcgtct ggatgttttc cactgcaaca agtctgcctt
     1861 ggcggataat gcctgccc
//
LOCUS       DAOSDA010000342         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_41158_length_1876_cov_3.421197, whole genome shotgun sequence.
ACCESSION   DAOSDA010000342 DAOSDA010000000
VERSION     DAOSDA010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41158_length_1876_cov_3.421197"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLW82_07575"
     CDS             <1..703
                     /locus_tag="PLW82_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ47171.1"
                     /translation="GQPCCHAACRIRTRSGLDCEESIAGFIEYAELYTKHNIPKAVDG
                     FMGFKNGELPLLQISSAQYSYIKTAAPELTGKWKVTVVPGTVREDGTISHAAYIGGST
                     FVMFKASKKQDEAFAWLKWWLSTETQTKYINEVPKALPGSVVIPANRHAMLNMPLPEE
                     DVEAYMLQASHSIAPAYALAPESITHRFINNACAKAVLQGVDPVEAILEAASLMNVEL
                     EKKQKEYARFISKLN"
     gene            842..1777
                     /locus_tag="PLW82_07580"
     CDS             842..1777
                     /locus_tag="PLW82_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOJ47172.1"
                     /translation="MIISASRRTDIPAFYSDWFFQRLDEGCFFVRNPMNPRQVSRVSF
                     TKEEIEGIVFWTKNPKNLMPKLNRLGSIPYYFQFTLTPYGRDLEPGLDKDQVVKTFKE
                     LSGMIGKKRIVWRYDPIVISPLMDANYHFSQFEALAEELGSFTEKCIVSFVDFYAKAR
                     KKLETIGAVDPSLEEKLAILRKLIQIAKANGLGIEVCCEAVLLGEIQVTRARCVDATL
                     LGEIAGENLAAEKDPNQRPECRCHVSVDVGAYDTCLWGCVYCYANGSAAQVQKKVKNY
                     RQDSPLLCSEIGALDVITERAVKPLKPRTRSLFLE"
BASE COUNT          497 a          451 c          511 g          417 t
ORIGIN      
        1 cgggcagccc tgctgccatg cggcatgcag aatccggacg cgctcgggct tggactgtga
       61 ggagtccatt gccggcttta ttgagtacgc agaactatac acaaaacaca acattcccaa
      121 agctgtagac gggtttatgg ggttcaaaaa cggagaactg ccgcttctgc agatcagctc
      181 cgctcagtac tcctacatca aaactgccgc accggaactt acaggcaagt ggaaggtcac
      241 agttgtaccc ggaacggtcc gggaagacgg gaccatcagc cacgctgctt acatcggtgg
      301 ctcaaccttc gtgatgttta aggccagtaa aaagcaggac gaagcctttg cctggcttaa
      361 gtggtggctt tcaacagaga cccagacaaa atacatcaac gaagtgccta aagctctgcc
      421 tggctctgtc gtgatccctg caaaccggca tgctatgctg aacatgcctc tacccgagga
      481 ggacgtggag gcctacatgc ttcaagcatc ccacagcata gcccctgcgt atgccctagc
      541 tccagagtcc atcacccaca ggtttatcaa caatgcctgt gccaaagcgg tgcttcaggg
      601 cgtcgatcca gtcgaagcta ttttggaagc agcaagcctg atgaatgtgg agctggagaa
      661 aaagcagaaa gagtatgcaa ggtttatcag caagctgaac taaggagagt ttccaagccg
      721 cacttaaaac cggtgcggct tttttgcaat acgagttctt agctcttcta gagggtttgg
      781 ggtctttttg gataatctaa aagaaggtct atgccaaaag caggagtagg gggggagtgc
      841 ggtgattatc agtgcaagca ggagaacgga tattccggcc ttctacagcg actggttctt
      901 ccagaggctt gacgaagggt gttttttcgt gcgcaacccc atgaatccga gacaggtgag
      961 ccgggtctcc ttcaccaaag aagagataga aggaatcgtc ttctggacga aaaaccccaa
     1021 aaacctcatg ccgaagctca accgcttggg atccatacct tactacttcc agttcacgct
     1081 taccccgtac ggcagagacc tcgagcccgg tctggacaaa gaccaggttg tgaaaacctt
     1141 taaggaactg tcggggatga ttggaaaaaa gcgcatcgtt tggcgttacg accccatcgt
     1201 catcagccct ttgatggatg caaactacca cttcagccag tttgaggctt tagcagagga
     1261 gctcgggtct tttacggaaa aatgcatcgt gagctttgta gacttctatg ctaaagctcg
     1321 aaagaagtta gaaaccatag gagctgtgga cccaagccta gaggaaaagc ttgcgatttt
     1381 aaggaagctt atccaaatcg ccaaagccaa cggtcttggc atagaagtat gctgcgaggc
     1441 ggtgctttta ggagagattc aggtgacaag ggcaaggtgc gttgatgcca cccttctcgg
     1501 cgagatcgcc ggggagaact tagccgctga aaaagatcct aatcagcgac ctgaatgccg
     1561 gtgccacgta agcgtcgatg tgggcgctta cgacacgtgt ctttggggct gcgtctactg
     1621 ctatgccaac ggcagcgccg cccaggtgca gaaaaaagtc aagaactaca ggcaggattc
     1681 acctctgctc tgcagcgaga tcggtgctct agatgtgatc acagagaggg cggtcaagcc
     1741 tttgaagcca agaaccaggt cgttgttttt ggagtagaag gggcagtcgg gacgggcaat
     1801 tttcaggtta tacatctttg tatcttcgcg taaagttttt gtagggtaga taaagaaaga
     1861 gaatagttcc ctgtgt
//
LOCUS       DAOSDA010000343         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_41304_length_1872_cov_3.397358, whole genome shotgun sequence.
ACCESSION   DAOSDA010000343 DAOSDA010000000
VERSION     DAOSDA010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41304_length_1872_cov_3.397358"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLW82_07585"
     CDS             complement(<1..209)
                     /locus_tag="PLW82_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47173.1"
                     /translation="MSIKPADMQVLLPRSQEIQKGEAVRDNRTNVNSQLVSSANFKET
                     LKNSTQVTKTQQRDMEQVRLKEDET"
     gene            complement(241..972)
                     /locus_tag="PLW82_07590"
     CDS             complement(241..972)
                     /locus_tag="PLW82_07590"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOJ47174.1"
                     /translation="MLSQDKVEESREELIKRYMYLVKEMARSFAKTLPSHIEEADLES
                     LGYLGLVDAVDKYDPKRGVPFEAYGRLRIKGAILDGLRKDDWLSEGARRKAKVIASVY
                     DELEVKLERPATDEEVAERLGIDVAEFRKIIQDVHREVISLETPVYTDGEGQTQVVMD
                     TLPAKEDISDELEKKQIKALIVDALQKLPEKERLVLALHYYEGLSLTEIAGIMELSTS
                     RISQLHAKAILRMRGRLGRKKALFF"
     gene            complement(989..>1872)
                     /locus_tag="PLW82_07595"
     CDS             complement(989..>1872)
                     /locus_tag="PLW82_07595"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOJ47175.1"
                     /translation="MQDQADGLRRMTGKNSTTKPSRKTKTIVVTSGKGGVGKSNFSLN
                     FSLALRQMGFQVGILDADLGLANLDVLMGVTAPYNITHLISGQKTLKEIILKCPGGVF
                     LIPGGSGIVSALIDERKLRVLLESLPVLDEYFDYLIIDTGAGISKQVLSFALAAEEVV
                     VVTTPDPTSLADAFNIIKVIHRGSADLPIGVVVNQADSTRHGEEVFSKLHKVSRNFLE
                     KDLYLIGIIPFDTTVSKAVRAQVPLLLYEKNAKSSLAIKRAASDYLMQGNLKASIPGK
                     EAGGLKSLLGKMVRMLR"
BASE COUNT          415 a          515 c          406 g          536 t
ORIGIN      
        1 ctcgtctcgt cttctttgag tctaacctgc tccatatcgc gctgctgggt tttggtcacc
       61 tgcgtagagt ttttcagcgt ttccttgaag ttggctgaag acaccagctg actgttgaca
      121 ttggttctgt tgtcccttac cgcttctcct ttttggatct cttgggaacg aggaagcaaa
      181 acctgcatat cagcgggttt gatcgacatg tgaacctcac cccttcagga tcaatcgaga
      241 ctaaaagaac aacgctttct tgcggcccaa ccgtcctctc atccgcaaaa tagcttttgc
      301 atggagctgc gatattcggg atgtagacag ttccataatc cctgctatct ccgtgagaga
      361 cagtccttca taataatgca gtgccaaaac caggcgttcc ttttctggaa gcttctgcag
      421 cgcgtccaca atcagtgcct taatctgttt tttctctaac tcgtcgctga tatcctcctt
      481 cgccggcagc gtgtccataa cgacctgagt ctgaccttca ccgtcggtat aaacgggggt
      541 ctctagagaa atgacctccc tgtgcacatc ttgaatgatc ttgcggaact cagcgacatc
      601 tatgccaagc cgctctgcca cctcttcgtc tgtggcaggt ctttccagct tgacttccaa
      661 ctcatcgtaa acactagcaa tgaccttagc cttccgtctt gcgccttcgc tcagccagtc
      721 gtctttcctc agaccgtcca gtatagcgcc tttgatgcgc agtctgccgt aggcttcaaa
      781 cggtacgccc cgcttaggat cgtacttgtc cacagcgtcg accaagccaa ggtaccccag
      841 gctttccaga tcggcttctt caatgtggct gggtagagtt ttggcaaaac tccttgccat
      901 ttctttcaca agatacatat agcgcttgat cagttcttcc ctgctctctt cgaccttatc
      961 ctgtgaaagc atatacccca tacctccttt agcggagcat ccgcaccatc tttcccagca
     1021 ggcttttaag cccaccggct tctttgcccg gaatgctggc tttaaggttg ccctgcatta
     1081 ggtaatctga agcggctctt tttatcgcaa gggaactctt ggcgtttttt tcgtagagca
     1141 gtaggggtac ttgtgcccta accgctttgc ttactgtagt atcaaaagga atgattccga
     1201 ttaaatatag atccttttcg agaaaattcc tgctgacttt gtgaagcttc gagaaaacct
     1261 cttctccatg cctggtgctg tcggcttggt ttaccaccac gccaataggt agatctgcac
     1321 tcccccgatg aatcacttta atgatattga aagcgtctgc aagagaagtt ggatccggtg
     1381 tcgtgaccac caccacttcc tctgcagcaa gggcaaagct cagtacctgt ttactaatgc
     1441 ctgcaccagt atcaataatg agataatcga agtattcatc caacaccgga agggactcca
     1501 aaagcaccct gagtttcctt tcatcgatga gtgcagacac gattcccgat ccccctggga
     1561 taaggaacac acctccgggg catttgagga tgatctcctt caaagtcttc tgaccgctaa
     1621 taaggtgtgt gatgttgtat ggtgcggtaa ctcccatcaa gacatcaagg tttgctagac
     1681 ccaaatcagc atcgagtatc cccacttgaa agcccatctg tctgagggca agggaaaagt
     1741 tgagtgaaaa attgctctta cctacaccgc ctttgccgct tgtaactaca atggttttgg
     1801 tcttgcgcga aggctttgtc gtactgtttt tccctgtcat tctgcgcagt ccgtctgctt
     1861 ggtcctgcat ct
//
LOCUS       DAOSDA010000344         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_42090_length_1848_cov_3.839375, whole genome shotgun sequence.
ACCESSION   DAOSDA010000344 DAOSDA010000000
VERSION     DAOSDA010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42090_length_1848_cov_3.839375"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PLW82_07600"
     CDS             <1..865
                     /locus_tag="PLW82_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007130174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ47176.1"
                     /translation="FPRWLADATTSMVKLFEIIDEEPEIIAVENPKNVPLEGKITFEN
                     VTFGYKSYEPVLKGIDIEIKPGEMIGLVGRSGAGKSTIINLLLRLYDPNQGRITINGV
                     DIREISPEYLHENIGVVFQDTFLFHGTLYDNIAYGKEGATPEQVIAAAKIANAHDFIM
                     QTSDGYNTIIGENGYTLSGGERQRIAIARAIIKNPSILILDEATSSLDVETESIIQES
                     LDRIVKGRTTIAIAHRLSTLKNADRLFVIDGGKIAEYGTHFELLKKKGIYYNLVMAQR
                     KTSKIAEPVEV"
     gene            complement(1002..>1848)
                     /locus_tag="PLW82_07605"
     CDS             complement(1002..>1848)
                     /locus_tag="PLW82_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47177.1"
                     /translation="VRETVVPFDDTLHSASRYFCYSGFLELREVEGTKYLLVYPELVL
                     LYSVINTEEFRADLRLHQNVDRYVRAATSLYGAVHLGQFFEIYNAHNRPISLYDVDRI
                     SAHLELSFKPYFLHKDYFLHDSFRDGLSDPDGRLSRWPTATEGIRNYFSPTVDFLKYV
                     DAEYVEETLQFLAVQDFLVDHVTGDISKANALTKRLYFKCAADSSLSDILKELERSKV
                     TISPKMLSGFVPLLVELDCSARKWRYKGFTYNELHQRGTGLATHRFYVELREKAGKLE
                     GDLRL"
BASE COUNT          480 a          462 c          472 g          434 t
ORIGIN      
        1 gttccccagg tggctggcag atgccacaac cagcatggtt aagctgtttg agatcatcga
       61 cgaagaaccc gagatcattg ccgtggaaaa cccgaaaaac gtccctcttg aaggcaagat
      121 tacctttgag aacgtcacct tcggctacaa gtcctacgag cccgtcctca agggtattga
      181 tattgagatc aagcccgggg agatgatcgg tcttgttggc aggtccggtg caggtaagtc
      241 cactatcatc aacctgctcc tgcgtctgta cgatccgaac cagggaagga tcaccatcaa
      301 cggcgtggat atcagggaga tctcgccgga gtacctccac gaaaacatcg gcgtagtgtt
      361 ccaggatacg ttcctcttcc acggaacgct ctacgacaac attgcctacg gtaaggaagg
      421 tgcaacccca gaacaggtca ttgcagcagc aaagatcgcc aatgcccacg acttcatcat
      481 gcagacgtca gacggctaca ataccatcat cggggaaaac ggttataccc tttcgggagg
      541 cgagaggcag agaattgcca tcgccagagc gatcatcaaa aacccatcca ttctcatcct
      601 ggatgaggct accagttctc tggacgttga gaccgagtcc atcattcagg aaagtttgga
      661 ccggatcgtt aaaggtagaa ccactatcgc catcgcccac aggctttcta cactgaaaaa
      721 cgccgacagg ctctttgtta tcgacggtgg caagatcgcc gagtacggca cccacttcga
      781 acttttgaag aagaagggca tctactacaa cctggtcatg gcacagcgaa agaccagcaa
      841 gattgcagag ccagtggaag tttaatagta aagcccctta agcttacagg acaataaaca
      901 cgcagaaaaa gagaatcact ccggagttct ccggtctgat tctctttctt ttgtccaaca
      961 tgtggtgtta tttgcacgga ctcttaccca aatgtttgtt cttaaagacg caggtcgcct
     1021 tctagctttc ctgccttttc cctaagctct acatagaagc ggtgggtggc aagaccggtg
     1081 cctctttgat gcagttcgtt gtaggtgaaa cctttgtacc gccattttcg ggcggagcag
     1141 tccagctcca ccaaaagggg aacaaatccc gatagcatct ttggggagat ggtcactttt
     1201 gatctttcaa gctcctttag aatgtccgag agggaactgt ctgcagcaca cttgaagtag
     1261 aggcgttttg taagagcatt tgccttcgat atgtctcctg tgacgtggtc cacaaggaag
     1321 tcctgcactg ccaagaactg aagcgtctct tcaacatact cggcatctac gtacttcaaa
     1381 aagtcgactg tgggcgagaa atagttacga atgccctcag tagctgtcgg ccagcggcta
     1441 agccgaccat ctgggtccga gagaccgtcc ctaaaggagt cgtgcaggaa gtagtctttg
     1501 tggagaaagt agggtttgaa gctgagttcg agatgggcag agatccggtc aacatcatag
     1561 aggcttatcg gcctgttatg ggcgttgtag atttcgaaaa actggccaag atggactgca
     1621 ccgtaaagac tcgtcgctgc tctgacgtag cggtcaacgt tttggtggag tcttagatcg
     1681 gctcgaaact cctcagtgtt gattacgctg tataataaca ccagttctgg gtaaaccagt
     1741 aggtatttgg taccttcaac ctctctgagt tcgagaaacc cgctgtagca gaagtacctg
     1801 ctggcgctgt ggagcgtgtc atcaaaggga acgaccgttt ctcgaacg
//
LOCUS       DAOSDA010000345         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_43273_length_1812_cov_2.608423, whole genome shotgun sequence.
ACCESSION   DAOSDA010000345 DAOSDA010000000
VERSION     DAOSDA010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43273_length_1812_cov_2.608423"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLW82_07610"
     CDS             <1..258
                     /locus_tag="PLW82_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOJ47178.1"
                     /translation="VIAESFARIFYRNAINIGLPILEVSIKDLVGDGDTVTVDLDGGT
                     VEVGGKTLKAQAFPPELMEIMQAGGLIAYVKKELGGGGSGE"
     gene            260..1300
                     /locus_tag="PLW82_07615"
     CDS             260..1300
                     /locus_tag="PLW82_07615"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOJ47179.1"
                     /translation="MKKIALIPGDGIGKEIVPVAWEVVKKATALKLESKVFDLGAERY
                     LATGEVFPEEIKEELKGFDAVFLGALGDPRVKPGGLERGILLTLRFEFDQCANLRPMK
                     LYREDLTPLKNLKQLDVMFFRENTEDLYIGAGGILKKGTPDEVALQELVQTRKGVERI
                     VRLAFEWAKKEGRRKVTLIDKSNVLTYAHDLWQRVFWEVASDYPELEANHLLVDAAAM
                     KMVLAPETLDVVVTTNMFGDILTDLGAAVCGGLGLAASGNINPDGLSMFEPVHGSAPD
                     IAGKNIANPLAAVLAGGMMLDHLGDSLGAKLIDDAVSFVLNEHQVTPDLGGSLTTTEV
                     GKALEAYLEERR"
     gene            1297..>1812
                     /locus_tag="PLW82_07620"
     CDS             1297..>1812
                     /locus_tag="PLW82_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOJ47180.1"
                     /translation="MRVAIYDTSLRDGTQREGLNLLAEDKFKLAKRLDSFGVDYIEGG
                     WPGSNPKDKEFFELCRDWTPKNAVVTAFGSTRKKNIKAEDDANLNAIVASGVKEAAIF
                     GKSWLFHVKEALNTTAEENLSMIRDSIEYLRAKGLGVHFLAEHFFDGYKADKEYALEC
                     LSAAVGAGAKSI"
BASE COUNT          457 a          423 c          547 g          385 t
ORIGIN      
        1 gtcattgccg agtcctttgc ccggatcttc tacaggaacg ccatcaacat tggtctcccc
       61 attttggaag tgtccattaa ggatctagtt ggagatggcg ataccgttac cgtagacttg
      121 gatgggggaa ctgttgaagt tggcgggaaa accctcaaag cccaggcgtt tcctccggaa
      181 ctcatggaga ttatgcaagc tggtggcttg attgcgtatg taaagaaaga actcggggga
      241 ggtggaagcg gtgagtgacg tgaagaagat cgcccttatt ccgggagatg gcatcggcaa
      301 ggagatcgtg cctgtggcct gggaagtggt gaaaaaagca acagcgctga agctagaaag
      361 caaggttttt gacctcggcg cagagcggta ccttgccacc ggagaggttt ttccagagga
      421 gatcaaagaa gagctgaagg ggttcgatgc ggtcttttta ggggctttag gcgatcctag
      481 agtcaagccg ggaggtttgg agaggggaat cctccttacg ctcaggttcg agttcgacca
      541 gtgtgccaac ctcaggccga tgaagctcta ccgcgaagac cttactccgc tgaaaaacct
      601 aaagcagctg gatgtgatgt ttttcaggga gaacacagaa gacctctaca tcggtgccgg
      661 cggaatcctg aaaaagggta cgccagacga ggtagctctg caggagttgg tgcagacgcg
      721 caaaggcgtg gaaaggatcg ttcgcctggc cttcgagtgg gccaaaaagg aaggccggag
      781 gaaggtcacc ctgatcgaca agagcaacgt cctcacctac gcccacgacc tctggcagag
      841 ggtgttttgg gaggtagcaa gcgattatcc ggagcttgaa gcaaatcacc ttttggtgga
      901 cgctgctgct atgaagatgg ttcttgcacc agagacgctg gacgttgtgg tgaccaccaa
      961 catgttcggc gacatcctca cagacctggg ggctgccgtt tgcggaggtc ttgggcttgc
     1021 cgcatcgggg aacatcaatc ctgacggact ttccatgttt gaaccggtgc acggttctgc
     1081 cccagatatt gccggaaaga acatcgccaa tcccctggct gcagttcttg caggcggcat
     1141 gatgcttgat cacctaggcg acagtctcgg agccaaactc atcgatgatg cggtctcttt
     1201 cgtactgaac gaacatcaag ttactcccga tttgggcggc agcctaacca ccacagaagt
     1261 gggtaaggcg ctagaggcgt acttggagga gagacggtga gagtagcaat ctatgatacc
     1321 tctctcagag acggcactca gcgggaaggg ctgaacctcc tggcagagga taagtttaag
     1381 ctggcaaaga gactggacag tttcggagta gactacatcg aagggggctg gcccggatcc
     1441 aatcctaaag ataaggagtt tttcgaactc tgcagggact ggactccgaa aaacgccgtg
     1501 gttacggctt ttggcagcac ccgcaagaag aacatcaagg ctgaagatga tgccaattta
     1561 aatgccattg tggcctctgg agtgaaagaa gcggctatct tcgggaagag ctggctcttc
     1621 cacgtaaaag aagcgctaaa cactactgca gaagagaacc tcagcatgat tagggactct
     1681 attgagtacc tcagagctaa aggtttgggg gtccacttcc tcgccgagca ctttttcgac
     1741 ggctacaagg cggataaaga gtatgcactg gaatgccttt ctgcagcagt gggcgcaggg
     1801 gcaaagagca tc
//
LOCUS       DAOSDA010000346         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_44441_length_1778_cov_2.853163, whole genome shotgun sequence.
ACCESSION   DAOSDA010000346 DAOSDA010000000
VERSION     DAOSDA010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44441_length_1778_cov_2.853163"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PLW82_07625"
     CDS             <1..716
                     /locus_tag="PLW82_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47181.1"
                     /translation="SLALCDLKLFIRNPSYTLNGLISYVLLPFLLVFQQLTLNVSLDE
                     LAGYFGGFHPSLIIVVCALYFAVMGMMSSMPSTPFSREGKYLWNLRSLPVSASQAMLA
                     KLVSSQIVNILGSLVGVVAFAVMFKLPLLPVFAGCIWGIVLSTGTSSLWVFLDLLRPM
                     LNWTNPIKAVKSNLNVIFSMVGMLGLILLVGSRAAHHLKTGTLTMMLVEFSVITLMVL
                     ALGYGLWKAFGENAYKSIV"
     gene            complement(838..>1778)
                     /locus_tag="PLW82_07630"
     CDS             complement(838..>1778)
                     /locus_tag="PLW82_07630"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOJ47182.1"
                     /translation="IRNLETAMKATKKYGGHAQGAVVYTTSPVHNIQHYVDMAKKLAD
                     LGADSICIKDMAGLLKPYVAFELVGAIKQAVDLPLQVHSHETTGLASMTYLKAIEAGA
                     DVIDTSLSALGSGSSQPATESIVATLMETEYDPGIDFELASEVNKHFKEVREKHKDKI
                     VPPWVNAEILRYQIPGGMLSNFFNQLKQLKLENKIDEVLAEVPRVREKLGYPPLVTPM
                     SQMVGTQAMMNVASPKGPWSIVLVDTKNYLKGEYGKPPGEIDEEFRRQIIGDEPVMTE
                     RPADRLEPGWEKAKAQFPSLSDEDVLSRVMFPELFK"
BASE COUNT          358 a          462 c          449 g          509 t
ORIGIN      
        1 tctctcttgc tctgtgcgat cttaagctgt ttatcagaaa tccctcttac acgctgaacg
       61 gcttgatcag ctatgtactg ctgccctttc tcctggtttt ccaacagctg accttgaatg
      121 tatccctaga cgagcttgcg gggtactttg gcggcttcca cccttcactg atcatcgtgg
      181 tttgtgcgct gtactttgca gtaatgggca tgatgtcttc gatgccctca acgccgtttt
      241 ccagagaagg caagtacctc tggaacttgc gctctttgcc ggtttcagcg tctcaagcga
      301 tgctggcaaa actcgtttca tcgcagattg taaacatcct cggctctctt gttggcgttg
      361 tggcatttgc ggttatgttt aagctgccgc ttcttccggt ttttgccggc tgcatttggg
      421 gtatcgtgct ctccacaggc acctccagct tatgggtgtt cctggatctt ctgcgcccca
      481 tgctcaactg gacaaatccc ataaaagccg ttaaatccaa ccttaacgtg atcttttcca
      541 tggtcggcat gttggggttg atccttttag ttggatcaag agcagcccac catcttaaga
      601 ctggaacctt gaccatgatg ctagttgagt tttctgtaat cacccttatg gttctggcat
      661 tgggatacgg cctctggaag gcctttggcg aaaacgctta caaatccata gtctaggacg
      721 gaacctacgc cgaggttaag gcagaagttg gcaaaagcac ttagggtaag ctagaatact
      781 ggatttaaac tttcaaagcg gatgtgcatc tttttgcaca tccgcttttt gaccagtcta
      841 tttgaaaagc tcggggaaca tcacccgcga gagcacgtct tcgtctgaaa gacttgggaa
      901 ctgagctttg gccttctccc agccgggctc aagcctgtct gcgggccttt cggtcattac
      961 cggttcatcg ccgatgatct gcctgcggaa ctcttcatcg atttcccctg gaggcttgcc
     1021 gtattcgcct ttgaggtagt ttttggtgtc taccagaacg atcgaccacg gtcccttggg
     1081 cgatgcgacg ttcatcatcg cctgggtgcc gaccatctgg ctcataggag tcaccagcgg
     1141 tggatagccc agcttctcgc gaacccgggg tacttcggca aggacttcgt cgattttatt
     1201 ctcgagtttc aactgcttca gctgattgaa gaaattcgac agcatgcctc ctggaatctg
     1261 gtagcgcaga atctctgcgt tgacccaagg cggcacgatc ttgtccttat gcttctcccg
     1321 cacttctttg aagtgcttgt tgacctccga agccagttcg aagtcgatcc caggatcgta
     1381 ctcggtttcc atgagcgtgg ccacgatcga ctcggttgca ggctggctgc taccgctgcc
     1441 tagagctgaa agagatgtgt caattacatc agccccggct tcgatcgctt tgaggtaggt
     1501 catagatgca agtcccgttg tctcgtgaga gtggacctgc agcggcaggt ctacagcctg
     1561 cttgattgcc ccgacaagtt cgaaggcaac atagggtttg agcagccctg ccatgtcctt
     1621 aatgcagatg gagtctgctc cgagatctgc aagttttttg gccatgtcta cataatgctg
     1681 gatattgtgt acaggagaag ttgtataaac aacggcacct tgggcatgac cgccgtactt
     1741 tttcgtagct ttcatagcgg tttccaggtt gcggatat
//
LOCUS       DAOSDA010000347         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_45009_length_1762_cov_4.934974, whole genome shotgun sequence.
ACCESSION   DAOSDA010000347 DAOSDA010000000
VERSION     DAOSDA010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45009_length_1762_cov_4.934974"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..830)
                     /gene="lexA"
                     /locus_tag="PLW82_07635"
     CDS             complement(207..830)
                     /gene="lexA"
                     /locus_tag="PLW82_07635"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOJ47183.1"
                     /translation="MSAKSELSKREEQILDLLKKGIAENGYPPTVREICAQTGLRSTA
                     TVHGYLKKLEQKGYIIKADDKPRAITLIGNEDFFSSSLEAALVPLVGEVAAGQPILAS
                     ENIEATYPLPKDLVKGEECFLLRVKGESMIEAGILPGDLVLVRRQQAASNGDIVVALL
                     EDEATVKTFYKEKNAVRLQPENSTMEPIYTKDVAILGKVIGLIRSMS"
     gene            1018..1299
                     /locus_tag="PLW82_07640"
     CDS             1018..1299
                     /locus_tag="PLW82_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ47184.1"
                     /translation="MRKQRIRSLLCVVVLVLIVSLLAIDKGKAGGAVQGKKISIVVEK
                     GDSLWSIAKKIAPDHDANEVIWYLMRLNGKENKIVREGELLIFDYATIN"
     gene            complement(1281..>1762)
                     /locus_tag="PLW82_07645"
     CDS             complement(1281..>1762)
                     /locus_tag="PLW82_07645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ47185.1"
                     /translation="ILPNTDWETRLLANRIMLESELEIIRSGGLTFFPGVEDTLRLLK
                     KKGYDLFVASNGLENYISAIVEHSGFGELFTDLYSAGRFQTKTKVDLVAKLLRDYPVT
                     TGYMVGDRKSDVEAGIKNNLPTIGCLFGYGGQKELEGATYIIESFSELPAIIQLIVA"
BASE COUNT          458 a          399 c          417 g          488 t
ORIGIN      
        1 gctgaaatca acggtggaag ttttgtaagt aaatccgaat acacggcatc gcgagatgaa
       61 aaatgagacc gtttaggtct cgtttttttt gcggtttgcc gaagcgattc accaaatttg
      121 aaaaactcct aaagaaaaag gagaggagca tagcaggaac agctagtcct ctccttattt
      181 ttatgtttgg ggtcgacttt tttggcttaa gacatactcc ggatcaaacc gattactttc
      241 cctaaaatcg caacgtcttt ggtgtagatc ggctccatgg ttgagttttc cggctgaagt
      301 cgcactgcat ttttttcctt atagaaagtt tttacagtag cttcatcttc aagaagggcc
      361 accacaatat cgccgtttga ggctgcttgc tggcgcctta ctaggaccaa gtcgcctggg
      421 agaatgccag cttcaatcat gctctcgcct ttaacgcgca gaaggaaaca ctcttcacct
      481 tttaccagat ctttgggcaa cggatacgta gcctctatgt tttcgcttgc cagtattggc
      541 tgtccagctg caacttcgcc aacgagggga accaaagccg cttctagaga ggacgaaaag
      601 aagtcttcgt tgccgatgag cgtgatagcc cttggtttgt catcggcttt gatgatgtag
      661 cccttttgtt ctagcttctt tagatagccg tgcacagttg ctgtagatcg cagtccggtc
      721 tgtgcgcaga tctccctgac ggtcggagga taaccgtttt cagctatccc tttcttgaga
      781 agatccagga tttgctcttc tcgcttagac agttcggatt tcgcagacac aagaacaccc
      841 cctaacctcc agaaaataca aagaaccaga ttaagccgca ctgtagatct cagctttagt
      901 ataacacagg ccgcctgata aaacaaacaa atgttcattt ttttcttgac acgaacatat
      961 gtgcggtata tgatgatatt gaacatatgt tcgcacaaca aaggtgaggt ggcttcagtg
     1021 agaaagcagc gcattagatc ccttttgtgt gttgtagtat tagtacttat cgtttcgctc
     1081 cttgccatcg ataaaggcaa agccggtggc gcggtccagg gtaaaaagat tagcattgtg
     1141 gtggaaaagg gagatagttt gtggagcatt gccaaaaaaa tcgcaccaga ccacgatgcc
     1201 aatgaggtca tctggtatct gatgcgatta aatggaaagg agaataagat cgttcgcgaa
     1261 ggagagcttt tgatctttga ctacgcaact attaactgaa tgattgcagg aagctcagaa
     1321 aagctctcaa taatataggt agcaccttct aactctttct gtccgccata gccaaagagg
     1381 cacccgatag tcggcaggtt gttcttgatt ccggcctcta catcggactt tctgtctccg
     1441 accatgtagc cggttgtaac aggatagtct ctgagcagtt tggctactaa atccactttg
     1501 gttttagttt ggaagcgtcc ggcgctgtac agatctgtga acagctcgcc aaaaccgctg
     1561 tgctcaacaa tggcggaaat gtagttttcc agaccgttac tggcaacaaa gagatcgtaa
     1621 cctttcttct tcagcaatcg cagagtgtct tctactccgg ggaagaaggt tagcccgcca
     1681 ctgcggatga tctcaagttc agactcaagc atgattcggt ttgcaaggag cctggtctcc
     1741 cagtctgtgt tcggcagtat ag
//
LOCUS       DAOSDA010000348         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_45210_length_1757_cov_2.982961, whole genome shotgun sequence.
ACCESSION   DAOSDA010000348 DAOSDA010000000
VERSION     DAOSDA010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45210_length_1757_cov_2.982961"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..963
                     /locus_tag="PLW82_07650"
     CDS             151..963
                     /locus_tag="PLW82_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47186.1"
                     /translation="MKVLVLLLLLTVSIYADPIIDLGVLTPGSDNVMELEIPLWIHSR
                     ENEVHYVRYEWRSDNFTTPPIWLKTEDGKVVRGEVVEVPVEKHDTSYPLYLSSDAFAP
                     PGTYQFTICTYSINPNSGSEYPHSRQYFTYAFTILPELLIEQTDLWPPPETGQPWESS
                     TVSKHTLRIKSRDPWELSIQGLKVVDGLHAVVFDKYGNVLSQPKIVPVTHDKQWVCEG
                     GPTVDMPDNEMVFTVVLYIEDYTVVPAGQAELQVYLEVRSFRQKSALLSVPD"
     gene            1152..>1757
                     /locus_tag="PLW82_07655"
     CDS             1152..>1757
                     /locus_tag="PLW82_07655"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOJ47187.1"
                     /translation="MAIVTSPVVNMYAKPNLFSETVSQAKMGDDVEILEDTDQSFFLV
                     KNLWDGYTGYVEKKYVDPGLTDWNPDPEVVTASLANLVYTGAKVQSPLLTRLPLGIRL
                     KLLNTQGAGIPDGWLRVEIPNDQTGFIQKGDTCTPEEFQFREISALRQSLVRWAKKFE
                     GAVPYLWGGTSSFGIDCSGFVQHIYKLHGLPLERDANQIGQG"
BASE COUNT          457 a          446 c          424 g          430 t
ORIGIN      
        1 tttatgaaca ccaattgtgg cgaggtctct cgcctactaa agcttgacgc gtactgacgc
       61 ctttcctgct tttgacagtt gccttcccag tctctataat tagactatag aggtacaatt
      121 cacaaaactc ctacatttgg tggtgttacc atgaaagttc tggtgctgct cttgctgttg
      181 acagttagca tatacgccga tcccatcatc gatttgggcg tcctgactcc aggctcggac
      241 aatgttatgg agttagagat ccccctctgg attcactcaa gagaaaacga agtccactat
      301 gtaaggtatg agtggagatc ggacaacttc accacgccgc caatatggct caaaactgaa
      361 gacggcaaag ttgttcgcgg agaagtagtt gaggttcctg tagaaaaaca cgatacatct
      421 tatccgctct acctctccag cgatgcgttc gcacctcccg gcacatatca gtttaccatc
      481 tgtacttatt ccattaatcc gaacagcgga agcgagtatc cacacagtcg gcagtatttt
      541 acctacgcct tcaccattct gccggagctc cttattgagc agaccgactt gtggcctcct
      601 ccagaaaccg ggcagccctg ggaatcctcg acagtgtcca aacacacgtt aaggattaaa
      661 tccagagacc catgggaact gtcgattcag gggctgaaag tggttgatgg actacatgca
      721 gttgtctttg ataagtacgg caacgttctg tcccagccca aaatcgttcc ggttacccac
      781 gataagcagt gggtctgtga aggtggaccg accgtcgata tgccagacaa tgaaatggtt
      841 ttcacagtcg ttctgtacat agaagactac acggtcgtgc ctgcgggaca ggccgaactt
      901 caggtttacc tggaggtcag atcgttccgc caaaaatccg ctttattgtc ggttcccgat
      961 tgaagcaact ctacagataa caaccttggc cggaaatcgt ggtcaaggtt gttttgcgtt
     1021 tagaacagaa aacctgtgct tctaactagc ttcccaaagg cttacgcaag gttgcagcga
     1081 acaattaact gtagttccaa actcctttgc cggagagctg catatcctag atctgaggga
     1141 gggagacttt cgtggcgatt gtaacatcac cagttgtaaa tatgtatgca aagccgaacc
     1201 tgttttctga aacggtgagc caagccaaga tgggcgatga cgtcgaaata cttgaagaca
     1261 ccgaccaaag cttttttctg gtaaaaaatc tgtgggacgg ctacacggga tacgtagaga
     1321 aaaagtacgt tgaccctggt ctcacagatt ggaatcccga ccctgaagtc gttactgctt
     1381 cactggccaa cctcgtgtac acaggggcaa aagtacaatc tccgcttctt acaagactac
     1441 ctctgggcat caggctgaag ctgctaaaca cccaaggggc aggcatccca gatggctggc
     1501 taagagtaga gatccccaat gatcagaccg gtttcattca gaaaggcgac acttgcactc
     1561 ctgaagagtt ccagttccgc gagatttcag ctctgcgcca gagccttgtc cgttgggcta
     1621 agaagttcga aggcgccgtg ccataccttt ggggagggac gtcgtctttc ggaatagact
     1681 gctcaggttt tgtgcagcac atctacaaac tgcacggtct tcctctcgaa cgggacgcaa
     1741 accaaatcgg ccagggc
//
LOCUS       DAOSDA010000349         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_45387_length_1752_cov_4.121391, whole genome shotgun sequence.
ACCESSION   DAOSDA010000349 DAOSDA010000000
VERSION     DAOSDA010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45387_length_1752_cov_4.121391"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..300)
                     /locus_tag="PLW82_07660"
     CDS             complement(130..300)
                     /locus_tag="PLW82_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ47188.1"
                     /translation="MPAFVHEEECIACGACADVCAVEAITVDEVAHVNPDICIDCGMC
                     VEECPTNCISME"
     gene            430..1191
                     /locus_tag="PLW82_07665"
     CDS             430..1191
                     /locus_tag="PLW82_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ47189.1"
                     /translation="MLASGSRGNSLYIETSGRRILIDVGISMRELTRRARQVEIDILS
                     VTDCFITHEHTDHICGIKTFAKNTASTIWTSKKLNSGCGIEETGDLTPNEPVVLGDVK
                     VTPIATFHDAVDPLGFVIETESKKALVLTDTGKRPKDLERYMDNLDLVVLEANHNKRM
                     LETGPYPYPLKRRITSNYGHLSNEESGEILADIVKRAKKVPTCFLAHLSEENNKPQIA
                     LLDVANTLKANEVLPGRDVQLEVASQYVPSKVLSI"
     gene            1203..1673
                     /gene="rlmH"
                     /locus_tag="PLW82_07670"
     CDS             1203..1673
                     /gene="rlmH"
                     /locus_tag="PLW82_07670"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOJ47190.1"
                     /translation="MVKIKILALGKIKEKYLRQGIDEYLKRLRPYAQVTVVEVEDLPI
                     PEREEDCRRMLQAEAEKLRPHLTGYKILLDIDGREVSSTEMAKELHDLVVRGNSQFTF
                     VIGSSLGVDEDFKKEFDFRWSFGPLTFPHQLMRLMLLEQLYRTFRILSGEPYHK"
BASE COUNT          504 a          406 c          437 g          405 t
ORIGIN      
        1 gaggcagtgt gggaacttct cctgaaacga gaacactgcc aaaggaaaag agctatcgga
       61 atagatccaa cagctctgtt tcacaggtgt ttgcaggttg gcgctgcaac ctagctgact
      121 tgtgatatgc tactccattg agatgcagtt ggttgggcac tcttcaacgc acataccaca
      181 gtcgatgcaa atatccggat ttacatgagc aacctcatct actgtgatag cctctactgc
      241 gcagacgtca gcacaagcac cacaggcgat gcattcttcc tcatggacaa atgctggcat
      301 taaaaacacc tcctgataat aagaacctta ttatcattct ctaagaggtt tcgaaatcct
      361 ttgtaattgt gataaaatct acagaggaaa taaaaacgca gcaagaggtg aagcacgatc
      421 aaaatatgta tcttagcaag cggaagcaga ggcaactcat tatatataga aactagcggc
      481 agacgcattt tgatagatgt aggtatctcc atgcgagaac tgacaagaag agcccggcag
      541 gttgaaattg atatcttgag cgtcaccgac tgcttcatta ctcacgaaca tacagatcac
      601 atctgcggta taaagacttt tgccaaaaac accgcaagca ctatctggac gtcgaagaag
      661 ctgaactcgg gctgcgggat tgaggaaacg ggggatctca ccccgaacga accggtggtt
      721 ttaggagatg ttaaagtcac tcccatcgct acgttccacg atgcggtgga tcctttggga
      781 tttgttattg aaaccgagtc aaaaaaagct ttagtcctta ccgatacggg caagcgccct
      841 aaggacttag aacggtacat ggacaatctg gatctggtgg ttcttgaagc aaatcacaac
      901 aagaggatgc tggagacagg cccctacccc tatcccctga agcggagaat cacctccaac
      961 tacgggcatc taagtaacga agagtccggc gagatacttg ccgatattgt caaaagggca
     1021 aaaaaagtac ctacctgttt tttggcccac ttaagtgaag aaaacaacaa gccgcagatt
     1081 gccctgttgg atgtggcaaa caccttgaaa gccaacgagg ttctgccggg gagagacgtg
     1141 cagctggaag ttgccagtca atacgtgccg tcgaaggttt tatctatcta ggagtggtcc
     1201 ccatggttaa gatcaaaatc cttgcccttg gcaagatcaa agaaaagtac ctcaggcagg
     1261 gaatcgatga gtatctgaag cggcttcggc cttatgctca ggtgaccgtt gtggaggtag
     1321 aggatctgcc cattcccgag agggaagaag actgcagaag gatgctgcag gccgaagcgg
     1381 aaaaactcag gccccatctt acaggctaca aaatcctttt agatatcgac ggcagggagg
     1441 tttcctcgac ggagatggcc aaagaacttc acgatctggt cgtccgggga aacagccagt
     1501 tcaccttcgt catcggcagc tcgctggggg tagatgagga cttcaagaag gagttcgatt
     1561 ttagatggtc ctttggacct ttaaccttcc cccatcagtt aatgcggctg atgcttttag
     1621 agcagctcta ccgcaccttc aggatcttaa gcggagagcc ctaccataag tgaggtcttc
     1681 ggcactctgc tacttgcttc ggtctgcagg gttgccattt aatggtattt aagaaaagca
     1741 tcgtggaaac ca
//
LOCUS       DAOSDA010000350         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_46560_length_1721_cov_3.634454, whole genome shotgun sequence.
ACCESSION   DAOSDA010000350 DAOSDA010000000
VERSION     DAOSDA010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46560_length_1721_cov_3.634454"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..286)
                     /locus_tag="PLW82_07675"
     CDS             complement(38..286)
                     /locus_tag="PLW82_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47191.1"
                     /translation="MKKALMLLVLVGLLSVGILAAENVNLLSYVESDSNFSGYRPDVT
                     NDGERNEGSEVNRWAEVAWASQESPGEHWLEYERSSPN"
     gene            complement(361..690)
                     /locus_tag="PLW82_07680"
     CDS             complement(361..690)
                     /locus_tag="PLW82_07680"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOJ47192.1"
                     /translation="MPKTIISELAPEIKVASSFFSRLKGFLGTVPKKGDALLLVPCRQ
                     IHTFFMKAPIDVYYLNRQGRVVKFFPNVKPWRILPYFPETYMVLELGAGTLPQFTEGE
                     IILEWKE"
     gene            complement(683..1357)
                     /locus_tag="PLW82_07685"
     CDS             complement(683..1357)
                     /locus_tag="PLW82_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ47193.1"
                     /translation="MRSQASVKVLLADDHPLVRQGLSKVLSLEEDIDIVAESVDGEDA
                     LRKVEEYLPDVVLMDVNMPRLNGIEATRRIKQRFNNVNVIALTFHDEDEYVLALMRAG
                     ARGYILKDAEPCIIATAIRKVYQGECYYPSNLINQVMENVDKKERKLGLEQDNVLTKR
                     ELEILEEIVQGKSNKEIADTLFISEKTVKNHVSNVLRKLDVTDRTQAAVHALRTGIVP
                     IRDLNA"
     gene            complement(1369..>1721)
                     /locus_tag="PLW82_07690"
     CDS             complement(1369..>1721)
                     /locus_tag="PLW82_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ47194.1"
                     /translation="INVTVLGKEVRLSGPIEIALFRLAQESINNAIKHSSCSQIDVKV
                     EFADQEIVLVVKDDGLGFDVEEKKSLSSENHHFGLVGMEERIRLLGGKFGIESVLGEG
                     TRVWCRVAYDTPGG"
BASE COUNT          433 a          447 c          347 g          494 t
ORIGIN      
        1 atcggataag cataatgata actgaggaat ttacacacta atttggactt gatcgttcat
       61 actcgagcca gtgctcgcca ggagactctt ggcttgccca tgctacttca gcccatctgt
      121 ttacttcaga accttcgttc cgttcgccgt cgttggtaac gtcaggacga taaccggaga
      181 agttgctgtc tgactcaacg taggaaagca agttcacgtt ctcggctgcg agtatgccga
      241 ctgacagaag accaaccaac actaacaaca ttaatgcctt tttcatcgtt aaacccccca
      301 caataatctt tgaatcaatg tatacattga tcttgtttat accattccac cttaactccc
      361 ctattccttc cactcaagga tgatttcgcc ttctgtaaat tgtggcagtg tacctgcccc
      421 aagttcaaga accatgtagg tttcaggaaa gtacggcaaa atgcgccagg gcttgacgtt
      481 tggaaaaaat tttactaccc taccttggcg gttcagatag tagacatcga taggggcttt
      541 catgaaaaac gtgtggatct gccggcaggg aaccagcaaa agagcatctc ccttcttggg
      601 gaccgtgccg agaaaaccct taagccgcga aaaaaaagag ctagcgactt tgatctctgg
      661 tgctagctca ctgatgattg ttttaggcat tcaggtccct tatgggtaca atccccgtcc
      721 ggagagcgtg tactgctgct tgtgttcgat ctgtaacatc tagttttctc agcacattgc
      781 taacgtggtt ttttaccgtt ttctcgctga taaacagcgt gtcggcaatt tccttgttgc
      841 tttttccctg aacgatctct tcgagaatct ccagttcgcg ctttgtcagt acgttatcct
      901 gttccagacc gagctttctc tctttcttgt ccacgttttc catgacctga ttgatcaagt
      961 tggaggggta atagcactca ccctgataga ctttgcgaat ggctgttgca ataatgcatg
     1021 gctctgcgtc ttttaaaata tagcccctcg ccccggctct catcagagcc agtacatact
     1081 cgtcctcatc gtggaaggtc agtgcaataa cgttcacgtt gttaaaacgc tgtttgattc
     1141 tccttgtggc ttcgatgccg tttaagcggg gcatgttcac gtccatcaaa actacatcgg
     1201 ggaggtactc ctcaaccttc cgtagggcat cttccccatc aacggactcg gccacaatat
     1261 caatgtcttc ctccaaactt aaaactttgc tcagaccctg cctcactaga ggatggtcgt
     1321 ctgctagcaa aactttaact gaagcttgag atctcatttt ccttgaattc accccccagg
     1381 agtatcgtag gcaactcgac accacactcg tgtcccctca cccaaaaccg attcgatacc
     1441 gaatttacca cctaaaagtc ttatccgttc ttccatgccg actaagccaa aatggtggtt
     1501 ttcagatgac agagattttt tttcttcaac atcaaaacct aaaccgtcat ctttcaccac
     1561 aagcacgatt tcttggtcgg cgaactctac cttgacatca atctgactgc aagaagagtg
     1621 cttaattgca ttgttgatcg actcttgtgc caaacggaaa agagctattt ctattggacc
     1681 tgaaagcctg acctctttgc cgaggacagt aacattaatg a
//
LOCUS       DAOSDA010000351         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_46715_length_1717_cov_3.265945, whole genome shotgun sequence.
ACCESSION   DAOSDA010000351 DAOSDA010000000
VERSION     DAOSDA010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46715_length_1717_cov_3.265945"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLW82_07695"
     CDS             <1..304
                     /locus_tag="PLW82_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47195.1"
                     /translation="ASITGMLGSETFYLIVTTSTPDLGVHFRPEIAHFVFSETQNPGK
                     RVFTVSGLTTTINRRYLLKRYTGEEWAPNVFIYATATVGIFKQGRFGFTYVPVLLK"
     gene            282..662
                     /locus_tag="PLW82_07700"
     CDS             282..662
                     /locus_tag="PLW82_07700"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOJ47196.1"
                     /translation="MYLYSSSKGQAMLELALLLPALVILLLAIFLVGQYFNLQICLDM
                     AVSAAIRAESLGYNGIEVFYEEWKKLTGRGQDSVQFRITKIAHVTVAYGCLELEMPSV
                     YQNFNLPNPKIESRLAHVFDILRM"
     gene            678..1343
                     /locus_tag="PLW82_07705"
     CDS             678..1343
                     /locus_tag="PLW82_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47197.1"
                     /translation="MLKIVVLCLMLLVSSVYAKPVQIGYTFSQDLTFPIAFMLEDKNK
                     LRGVDFYDSSQVLEFIGSINPTLLVNQAKLDSLSEQVEAFAKGDAAIVLEGTDKAVYM
                     DIGKRKPSSSVVWKYLNLSEIEYAIGFANMEAIQLNHNHVDRLCSLLKQNEKIRYPDD
                     YDFCRDFGLELFDIGDESIVIFATSSEPKAMIQVTRDDQEAIAVGDFIRDRFSQLVFS
                     GWN"
     gene            1288..>1717
                     /locus_tag="PLW82_07710"
     CDS             1288..>1717
                     /locus_tag="PLW82_07710"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HOJ47198.1"
                     /translation="MGISFAIVFLSWFFLAGTNGNDGDEPTTVPVLVAREFLSAGTRL
                     KGSDFEEVGLPVELLPEGVAGFDIEGKVLSYPLQKGEILFAKDLEGIQTEAKVMLTLQ
                     TDALGISQGDKIAVLVSSEEDTLTLADNLQVEEVRRNEYSD"
BASE COUNT          464 a          375 c          429 g          449 t
ORIGIN      
        1 tgcatcaata accggtatgc ttggcagtga gaccttctat ctgatagtta ccacatcgac
       61 tcctgacctt ggggtgcact tcagacccga gatagctcat ttcgttttta gcgagacgca
      121 aaaccctgga aaaagagttt ttaccgtttc gggtcttacc accaccatta accgccgcta
      181 tctgcttaag cggtacaccg gtgaagagtg ggctccgaac gtatttattt atgcaaccgc
      241 cactgttggc atattcaagc aagggagatt tggatttacc tatgtacctg tactcctcaa
      301 gtaagggaca agctatgttg gaactggctc ttttactgcc ggctttggtg atactgcttt
      361 tggcgatctt tctcgtgggg cagtacttca acttacagat ctgtttggat atggctgtgt
      421 ctgcagctat tcgtgccgaa agcctcggtt ataacggaat cgaagtcttc tatgaagaat
      481 ggaagaaact gacaggtagg ggccaagact ctgttcagtt ccgcataacc aaaatagccc
      541 acgtgaccgt tgcttacggc tgcctcgaac tggaaatgcc cagtgtatac cagaacttca
      601 atctacccaa tccgaagatc gagtcaagac tggctcatgt atttgacatt ttgcggatgt
      661 agaaaggagt ggcaaagatg ttaaaaatcg tcgttctctg tctgatgctt ttggtctcaa
      721 gcgtttatgc caagccggtg caaatcggct acaccttctc tcaggatctt acatttccaa
      781 ttgcttttat gttagaagac aaaaacaaac tgagaggagt ggacttctac gattccagtc
      841 aagtactgga gttcatcggc tcaatcaatc ccaccctttt ggttaaccaa gccaagttag
      901 attcgctctc agaacaggtt gaagctttcg caaaaggaga tgctgccata gtgcttgaag
      961 gcacggacaa agcggtgtac atggatatag ggaaacgaaa acccagtagc tcagtggttt
     1021 ggaagtactt gaacttgagc gaaatagagt atgctatcgg ttttgccaac atggaagcga
     1081 tccagttaaa ccacaaccat gtggacaggc tgtgctctct gttaaagcaa aacgaaaaga
     1141 tccgctatcc tgatgattac gacttctgtc gagactttgg gttggagttg ttcgacatcg
     1201 gtgacgaaag tatcgtgatt tttgccacaa gcagcgaacc taaagctatg atacaggtga
     1261 cgagagatga ccaagaagct attgcggttg gggatttcat tcgcgatcgt ttttctcagc
     1321 tggttttttc tggctggaac taacggcaat gatggggacg agcccacaac cgttcccgta
     1381 ctggttgcaa gagagtttct ttctgcggga acgcggctaa agggatctga ttttgaggaa
     1441 gtcggcttac ctgtagaact gctacctgaa ggagtggcag gatttgacat tgaagggaaa
     1501 gtactcagct atccactgca aaaaggtgaa attctctttg ctaaagactt ggaaggcata
     1561 caaaccgaag ccaaggtgat gcttactctc cagaccgacg cgttgggaat cagtcaggga
     1621 gacaaaattg cggtgcttgt aagtagtgaa gaagacactc tcaccttggc agacaacctc
     1681 caggtggagg aagtgcgccg caacgagtac agcgata
//
LOCUS       DAOSDA010000352         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_46881_length_1713_cov_3.065742, whole genome shotgun sequence.
ACCESSION   DAOSDA010000352 DAOSDA010000000
VERSION     DAOSDA010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46881_length_1713_cov_3.065742"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="PLW82_07715"
     CDS             complement(<1..1712)
                     /locus_tag="PLW82_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HOJ47199.1"
                     /translation="MIMHKNPLERIRFTQELQEVFEQAVKIIVPESRQQLIEMAVGYD
                     ARGYFEVVSEIPGSGKIVEATVARCRNGIAVNYQDVYMRRRDPDAIMVADDRPTDKVR
                     YQDRYGEDFAPLRKATLKWLGTQNLIVMPFMAGGSQDLGYPAVLIAPENTAFFAAALA
                     DLQGMIPASQLPDGFEPRSIIYLAPPFRHTHFGGKQVVLHHKNDGVHEIFSYNLYPGP
                     SAKKGVYGMLLSIGEEEGWITAHASTVRVISPYDNVVTFMHEGASGGGKSEMLEEPHR
                     QPDGKILFATNILTNEDIYFDMRDTCELEPVTDDMAFCHPSLQKGSKKLVVRDAEAGW
                     FIRINHITDYGIAPHYERLTINPKEPLVFLNIDGVPNATCLIWEHTMDEPGKRCPNPR
                     VIMPRKLIPNTVDDAVEVDIRSFGVRTPPCTADKPSYGIIGLFHVLPPALAWLWRLVA
                     PRGYDNPSIIESGKGMTSEGVGSYWPFATGRMVDQANLLLDQILNTLSTRYLLIPNQY
                     IGAYKVGFAGQWITREYMARRGSAKLRPEQQVPARCPLLGYALQNIKVDGVNLPKGLL
                     QVNL"
BASE COUNT          363 a          439 c          458 g          453 t
ORIGIN      
        1 tgaaggttga cctggagaag acccttcggc aggttcactc cgtccacctt gatgttctgc
       61 agtgcatacc ccaagagagg acaccttgct ggcacctgct gctcaggtct gagcttagcg
      121 ctgccccgtc ttgccatata ctctctggtg atccactgtc cggcaaagcc cactttgtag
      181 gcaccgatat actggttggg tatgaggaga tatcttgtag acagggtgtt caggatctgg
      241 tctagaagaa ggtttgcctg gtcgaccatc cttcctgtgg caaacggcca gtaggagccg
      301 acaccttcgc tggtcattcc tttacccgac tcaatgatgc tgggattgtc atagccccta
      361 ggtgctacca gcctccagag ccatgctaga gccggaggta gaacatgaaa gagtcctata
      421 ataccgtaag aaggtttatc ggcggtgcat ggcggagttc tcacaccaaa gcttctgatg
      481 tctacttcca ctgcatcgtc gactgtattg ggaatgagct tcctgggcat gatcactctc
      541 ggattaggac agcgcttgcc gggctcgtcc attgtatgct cccaaatcaa gcaggttgca
      601 ttgggtaccc cgtcgatgtt taaaaacacc aatggttctt tgggattgat ggtcaggcgc
      661 tcgtagtggg gcgcgatgcc atagtcggtg atgtggttga ttctgatgaa ccagcctgct
      721 tctgcgtccc gaaccaccag ctttttgctg cccttttgca aactggggtg gcagaaagcc
      781 atatcgtcgg ttaccggttc tagctcgcag gtgtctctca tgtcgaagta gatgtcttcg
      841 ttagtcagga tgtttgtggc aaagagaatc ttgccatcgg gctggcggtg tggctcctcg
      901 agcatctcgc tctttccgcc gccacttgcg ccttcatgca taaacgttac tacgttatcg
      961 tagggactga taacccgaac cgttgacgca tgagcagtga tccagccctc ttcttctccg
     1021 atagaaagaa gcatgccgta gacgcccttt ttggcgctag gtcctggata gagattgtat
     1081 gagaaaattt cgtgaacgcc gtcgtttttg tggtgcagaa ctacctgttt gcctccgaag
     1141 tgggtgtgtc tgaagggagg tgcaaggtag ataatggacc tgggctcgaa gccatcggga
     1201 agctgacttg cggggatcat tccctggaga tcggcaagag ccgcggcaaa aaacgcggtg
     1261 ttttcaggtg caatgagcac tgcaggataa ccaagatcct gactgcctcc tgccataaaa
     1321 ggcatgacga tcaggttctg ggtacccagc cacttgaggg tcgcttttct cagcggggcg
     1381 aaatcttcgc cgtacctatc ctgatatctc accttatcgg tgggacgatc gtctgcaacc
     1441 ataatggcgt cgggatctct gcggcgcata tacacgtcct gatagtttac ggcaatgcca
     1501 tttcggcacc tggctacggt cgcttcaacg attttcccgc ttccgggaat ctcgctcacg
     1561 acttcaaaat agccgcgtgc atcgtagcct acagccattt caatgagctg ctgacggctt
     1621 tcaggtacga ttattttaac tgcttgttca aagacttctt ggagttcctg ggtaaatcgt
     1681 atcctctcta gcgggttttt gtgcataatc atc
//
LOCUS       DAOSDA010000353         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_47284_length_1703_cov_2.978155, whole genome shotgun sequence.
ACCESSION   DAOSDA010000353 DAOSDA010000000
VERSION     DAOSDA010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47284_length_1703_cov_2.978155"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLW82_07720"
     CDS             complement(<1..570)
                     /locus_tag="PLW82_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOJ47200.1"
                     /translation="MIPELSGSLRKNQIIIPSIINEASGVRINGKLIKSLLFSTDVAV
                     IRNTNADAVIAVYPFSPQPVITEALRIAADVPLFVGVGGGTTAGLRVVNLALHAEFQG
                     VMGVVLNAPIKNEVLARVAETIDIPIVITVVSENEDIEARLRHGASILNVSAAKNTPD
                     VVRKIRERFPDVPIFATGGPTDETIAETIA"
     gene            665..1237
                     /locus_tag="PLW82_07725"
     CDS             665..1237
                     /locus_tag="PLW82_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOJ47201.1"
                     /translation="MSFDALVSNLHINSYLDVLKAQSISLLGKVVIAIVVFAAGWLVI
                     RYLKMLFNRVLRRGRVDQALVKLMSSLLTFVGWIIVISAVFKVLGFTEVSLAFSGSLA
                     LIAMGLANSASSIVGDLFSGVTLIADDDFEIGQRVTAAGITGTLEAIDMRKTRIRDDN
                     GNLYIIPNRSIDGGTITVVKPAEEKSKSAS"
     gene            1434..>1703
                     /locus_tag="PLW82_07730"
     CDS             1434..>1703
                     /locus_tag="PLW82_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47202.1"
                     /translation="MRNILIISLLAALAVSLTSCYIMREEVTGEVPPEPYYACFPGSY
                     YRKAVSSFDQWTGIEGIIVLPEHEPDIRRWGSTGPLDNFSIYMGGR"
BASE COUNT          421 a          391 c          445 g          446 t
ORIGIN      
        1 tgcgatggtt tctgcaatgg tttcgtctgt tgggccgccg gtggcaaaga tgggaacatc
       61 agggaaacgt tccctaatct ttctcactac atccggtgtg ttctttgcag cggatacgtt
      121 gagaatgctg gcaccgtgcc tgagccttgc ctcaatgtcc tcattttcgg agacgacggt
      181 aattacgata ggtatgtcaa tagtttcagc gactctggcc aaaacttcat ttttaatcgg
      241 tgcgttcaaa actacgccca taacgccctg gaactctgcg tgaagggcga ggttgaccac
      301 tcggagtcct gctgtggtgc cgcccccaac ccctacaaaa aggggaacgt ccgcagctat
      361 tctcagcgct tcggtgatca ccggctgcgg tgaaaaggga tagactgcga taaccgcatc
      421 ggcgttagtg ttgcggatga ctgccacatc tgtggaaaac agcagggact tgatcagctt
      481 tccgttgata cggactccgg aagcttcatt gattatactc ggtataatga tctggttttt
      541 tcttaacgat ccactgagtt caggtatcat agcaaacctc ccaaatttcg ttttggttaa
      601 aggataaact gcatcagaag ctgaatagat gaaagaatac taaatgcagc ggagggtaga
      661 aaagatgagt tttgatgcac tagtcagcaa tctgcatatt aatagctatt tggatgtgct
      721 taaggctcag tccatttcac tgcttggcaa agtggtcatt gctattgtgg tttttgctgc
      781 cgggtggctg gttatccgct acttaaagat gcttttcaat agagtcttgc gcagaggccg
      841 tgtcgatcaa gccttggtaa aactcatgtc ttcactgctc acctttgtgg gctggatcat
      901 cgtgatctcg gcagttttta aggttttagg ctttactgaa gtgtctctgg ccttcagcgg
      961 ttcgctggca ttgatagcaa tgggtttggc caactcggca tcgtccattg tgggcgatct
     1021 tttcagcggt gttacgctga ttgccgacga tgacttcgaa ataggccaaa gggtaaccgc
     1081 tgcgggaatc accggaacac tggaagccat cgatatgcgt aaaaccagaa tacgcgacga
     1141 caacggcaac ctgtatatca tacccaacag gagcatagac ggcggtacca tcaccgttgt
     1201 gaagccggca gaggaaaaaa gcaagagtgc ttcttgacgg gcggagtttg accccgtcta
     1261 acagtattct atttaaaggt tttttttcct agcacagaat gggaatagaa ccagtgtttg
     1321 aaaacgaggc gtaaaacaaa gctccgagag agaatgccgg acgcctcttg ccacacgcca
     1381 tttacgattt ggagagatag atgctgatga gaaagattgt aggaggggta tctgtgagga
     1441 acatccttat cataagtctg cttgctgctc tagccgtttc gcttaccagc tgctacatta
     1501 tgcgagaaga ggtcaccggc gaggttcccc ctgagcctta ctacgcctgc ttcccagggt
     1561 catactaccg caaagcggtg tccagctttg atcaatggac cggtatagaa ggaatcattg
     1621 tccttcctga gcacgagccc gacattagaa ggtgggggag cacaggcccc ttggacaact
     1681 tttccatcta catgggtggc aga
//
LOCUS       DAOSDA010000354         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_47694_length_1693_cov_3.412698, whole genome shotgun sequence.
ACCESSION   DAOSDA010000354 DAOSDA010000000
VERSION     DAOSDA010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47694_length_1693_cov_3.412698"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLW82_07735"
     CDS             <1..498
                     /locus_tag="PLW82_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47203.1"
                     /translation="DICKLAVGLDPENYLPWSLLSQEYAETMQYKKGLETGMRALELN
                     PRDSYAHNAVAKCYEGIGEVEKARHHFMLRARLQNTPDGYRIVTDYLERMGEINSALD
                     MARTWLELEPRNPLAYQRVEQLSERLGSQEYAYSDILKHESGIKRKNAMEEEVIYRAV
                     ATGES"
     gene            567..>1693
                     /locus_tag="PLW82_07740"
     CDS             567..>1693
                     /locus_tag="PLW82_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ47204.1"
                     /translation="MTTIEGTVERITYRNEDNFYTVFKFKVGTDDIYTCVGNLAQITA
                     GEELRLTGEWVRHNVYGLQFSVESALPITPSTLVGIEKYLGSGLIKGVGPATAKKLVD
                     AFGMETLKVIEETPHRLTEVDGIGEKKAEVIASAFQAQKDIQDVMVFLQSNGITPLLS
                     MKIYKAYGKDSVDVVKENPYRLAEDIFGVGFKTADKIALSLGLREDDPNRLRAGLKYA
                     LGQAVEQGHTYLPKDELLEEAKKLLGLEIPESLIEQLAAARQLIVEDSKCYLPSLYEA
                     EQRVAKALVLLAKQVATLPFDDLQIESEFELAPEQKEAVKKAMQEKLLVITGGPGTGK
                     TTITKVILDQWEKAGFKVLLASPTGRAAKRLAEAMGHLVKT"
BASE COUNT          493 a          368 c          469 g          363 t
ORIGIN      
        1 gacatctgca aactagcggt gggactggac cccgagaact acttgccgtg gtctctactc
       61 tctcaggaat atgccgaaac catgcagtac aaaaagggtc tggagacggg aatgagggcg
      121 ctagaactca accctagaga ctcctatgcg cacaatgcag tggctaagtg ctacgaaggt
      181 attggtgagg tggaaaaggc cagacaccac ttcatgttgc gcgcaaggct ccaaaacacc
      241 ccagatgggt accgcatcgt tactgattat ctggagcgca tgggggaaat caattcggca
      301 ttggatatgg ctagaacctg gttagagttg gagccaagaa atcctttagc ctatcagagg
      361 gtagaacagt taagtgaaag attgggttct caagagtacg catattctga tatactgaaa
      421 catgaaagcg gtataaagag gaaaaacgcg atggaagaag aagtgatata tcgagctgta
      481 gcaacaggag aaagctagaa tcggctacaa acaaggcggc ttaggccgcc ttcatttatc
      541 agtcaccggt gcaggaggtg aggcttgtga ccactatcga gggaactgtg gaaagaatta
      601 cctatcgcaa cgaggacaac ttctacacag tttttaaatt caaggttgga acagatgaca
      661 tctacacgtg cgtaggtaat cttgcgcaga tcactgccgg ggaagagctt aggcttacgg
      721 gagagtgggt aaggcacaac gtgtacgggt tgcagtttag cgtggaaagc gctctgccca
      781 tcactccgtc cacgctggtt ggtatcgaaa aatacctggg gtcgggattg atcaaaggtg
      841 tgggaccggc taccgccaag aaactggtgg atgcgttcgg aatggaaaca cttaaggtca
      901 tcgaggagac tccccaccgg ctcactgaag tcgacggtat cggtgagaaa aaggcggagg
      961 taatagccag cgcatttcaa gcacaaaagg atattcagga tgttatggtc ttccttcaga
     1021 gcaacggcat taccccattg ctttcgatga agatctataa agcgtacggg aaagactcgg
     1081 ttgatgtggt caaagaaaac ccctacaggc tggctgaaga catctttggt gtgggtttca
     1141 agaccgcaga taagatcgcg ctcagtctag ggctcagaga agacgatccc aaccgcttga
     1201 gagcagggct gaaatacgcc ttaggacaag cagtggaaca gggacacacc tatctgccca
     1261 aggatgaact tctggaagaa gcaaaaaaac ttcttggtct cgagattcct gaaagcttaa
     1321 ttgagcagct agccgctgcc cgccagctga ttgtggaaga ttcaaagtgc taccttccaa
     1381 gcctctacga agctgaacag agggtagcta aggctttggt ccttctagcc aagcaagtgg
     1441 caactttgcc ttttgacgat cttcagatag aatccgagtt tgaattggcg ccggagcaga
     1501 aagaagctgt caaaaaagcc atgcaggaaa agcttttggt gattaccggt ggcccaggta
     1561 caggtaagac caccattacc aaagtcattc tggaccaatg ggaaaaggca ggttttaaag
     1621 ttttactggc ttcgcctaca gggcgagctg ccaagaggct tgccgaagcc atggggcatc
     1681 tagtcaagac tat
//
LOCUS       DAOSDA010000355         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_48188_length_1682_cov_2.758451, whole genome shotgun sequence.
ACCESSION   DAOSDA010000355 DAOSDA010000000
VERSION     DAOSDA010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48188_length_1682_cov_2.758451"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..>1682)
                     /gene="nrdD"
                     /locus_tag="PLW82_07745"
     CDS             complement(83..>1682)
                     /gene="nrdD"
                     /locus_tag="PLW82_07745"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOJ47205.1"
                     /translation="IHDLDSYGKTFNCMQIPLDQLLEKGFSTGYGYIRPPKRIGSAAA
                     LMCIIIQSSQNDMYGGQSVPWMDKTLAKYVDPRYDDLLRDNLTDEEQALKKSIEKEVY
                     QAMEATIHNLNTMHSRAGSQVPFSSVNFGTDTTPRGRLVTEQFLKAFYAGLGRGETPI
                     FPNVVFRLKEGVNFNPGDPNYDLYQLALKVAGRRMNPTFSFMDSSMNKPFGDRASYMG
                     CRSRLMDNIYGESTPEGRGSIAPVSINLPRLALESCGSKDNFFEKLDEILALCRRQLL
                     HRVEVLKERTVSDIPFVMGEHLYMGSEGLNPNDSIWEAMKHGSVNIGFIGLAEALILL
                     TGNHHGENEAAQELGLEIVRRIRAYCDRITEEDQLNCTCYATPAEGLSGRFIAIDKKI
                     FGTIPGVTDKDYYTNSFHIPVNYNISAFKKISIEGPYHKYCNAGHISYVEVEAPLENN
                     PEALESLHRAMSAADMGYGGVNFPIDECVDCAHSGIIDLECPVCGSQRINRVRRVTGY
                     LSYLHRFTTGKKAEYQNRRPHEKS"
BASE COUNT          366 a          403 c          433 g          480 t
ORIGIN      
        1 tccgctccag cagagagcag ttgcctgaac agcctgctcc caagacctta atgttcagag
       61 gttggctcaa aaaaccgcct ccttaagatt tctcgtgcgg acgcctgttt tggtactcgg
      121 ctttcttgcc agtggtaaag cggtgtaggt agctaaggta acctgttact ctgcggacgc
      181 gattgattct ctgtgatccg cacaccggac actcgagatc tatgatgccg ctgtgggcgc
      241 agtcaacgca ttcatcgata gggaagttca ctccgccata gcccatgtca gctgcgctca
      301 tagctctgtg caggctttcc aaagcctctg ggttgttttc cagaggagct tctacttcga
      361 cgtacgaaat gtgacctgcg ttgcagtatt tatgatacgg tccttcaatg ctgattttct
      421 tgaatgcgga gatgttgtag ttcactggaa tgtggaagga attggtgtag tagtctttat
      481 cggtaacacc ggggatggtg ccaaagatct ttttgtcgat ggcgatgaat cgtccgctca
      541 acccctctgc aggagttgca tagcaagtgc agttcaactg gtcttcttca gtgattctat
      601 cgcagtaagc tctgatgcgg cgcacgatct ccagcccgag ctcttgagct gcttcgtttt
      661 caccgtggtg gtttcctgtg agaaggatca gagcttcagc aagaccgata aagccgatat
      721 tgaccgagcc gtgtttcatg gcttcccaga tactgtcgtt tgggttcaac ccttcagaac
      781 ccatatagag gtgttcaccc atgacaaaag gtatgtcgct tacagtgcgc tctttcaaca
      841 cttctactct gtggagcagc tgtcttcggc acaaagctag aatttcatca agcttttcga
      901 agaagttatc tttgcttccg caggactcta gtgcaagtct gggcaggttg atggatactg
      961 gtgctatgct gcctcttcct tcaggggtag attcgccgta aatgttatcc atgagacggc
     1021 tcctacaacc catgtagctt gctctatcgc caaaaggttt gttcatgctg gaatccataa
     1081 acgagaacgt tgggttcatt ctcctgccag cgactttgag tgccagctgg tagaggtcgt
     1141 agtttggatc tccagggttg aagtttacgc cttccttgag cctgaagact acattgggga
     1201 aaatcggtgt ctcgccacgg ccaaggccag catagaacgc tttcaaaaat tgctctgtaa
     1261 ccagccttcc cctaggagtg gtgtcggttc cgaagttcac cgatgaaaac ggtacctggg
     1321 aacctgctcg tgaatgcatg gtgttgagat tgtggattgt ggcctccata gcttgataga
     1381 cttctttttc gatgctcttt ttaagtgcct gttcctcgtc tgtgagatta tctctgagaa
     1441 ggtcgtcgta ccttgggtca acgtacttgg ccaaggtctt gtccatccac gggacagact
     1501 gtcctccgta catatcgttt tgcgaagatt ggatgatgat gcacatcaat gctgctgcag
     1561 acccgatgcg tttaggcggt ctgatgtaac catagccggt ggaaaagccc ttttccagaa
     1621 gttgatccag agggatctgc atgcagttaa aggtttttcc gtagctgtcc aagtcgtgaa
     1681 tg
//
LOCUS       DAOSDA010000356         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_48279_length_1680_cov_2.752000, whole genome shotgun sequence.
ACCESSION   DAOSDA010000356 DAOSDA010000000
VERSION     DAOSDA010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48279_length_1680_cov_2.752000"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..962)
                     /locus_tag="PLW82_07750"
                     /pseudo
     CDS             complement(1..962)
                     /locus_tag="PLW82_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(1250..>1680)
                     /locus_tag="PLW82_07755"
     CDS             complement(1250..>1680)
                     /locus_tag="PLW82_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47206.1"
                     /translation="GAYYPGSLVKRIREDGGRLLNALVEKKALSVSNIDVCGSLFKVE
                     EGYVVHLVNTTGILPEDEGYVTRTDKVLPFSEEGEKLGQLLLNVQVEDLGTVEEIKLY
                     SPEFSGERTAKFSQAGGNVSIEIPEGLFSGYLLVHLIGGK"
BASE COUNT          411 a          464 c          389 g          416 t
ORIGIN      
        1 tacacctgtt taatgtcttc agggaggtta acctgcatca tagcttcttt attggccgga
       61 agccacatat atccagaact cttagccaga atctcgttgg ccactttggt cagaacatca
      121 ctgcccaaga accacttgag aaactgccag gcttcttcct gcctcttaga gtaagcggaa
      181 atgcccagaa ggacgctgcc tgcagacgag ccgttgtgaa gggttccgtc ccgttcaact
      241 cctggtacca tgtcgacctt ccacttgccg gtcagctgag gtgccgccat actcaagttg
      301 gtgtaagtcc acaaaccatc aacataggtt atctgttcgc cgctgataaa cggcgttatc
      361 ccagcaagag ctttcgggaa gtcgtgcttg gtgaacagct caacgtagtt gataaaacct
      421 ttgatggact cgggcttgtc tagagcactg gaaaaaccgt cgggattgaa gaactgacct
      481 ccatgctggg tcatcaccgt atagcatgcc cacagctcat cgaagttcat aaaagcaaag
      541 gtcttgcctc ttgccagggc ttttggctat gcgtttacga tctcctccca tgtcttgggt
      601 atgtccatgc ccatctccgc taaaatgtcg gcacggtagg ctcccacaag tgccgccaca
      661 tccatgggaa gggcaaaacg gtgggcttca aaagtaaacg gtcccattac cgacgagaag
      721 agcttctttt ccaactctgc gtactcttca ggcttgaaag cacccagatc gattaagcca
      781 ccccgtacgg caaagtcggc aatttcagca ccgaaactga agatatccgg cgtatctctg
      841 ctggcaaggg ctaagaggga cttggtatag tagtcgcccc aagccagcgg aacgatatct
      901 acttcgatac ctgtctcggc agtgaacaga tcgtccacca acgcctgacc gatctgcccc
      961 atttcgttac tccacccaac taaccagacc tccagcggtt tggcgaagac gcatacgctg
     1021 cttagcacca acaggaggaa aagcaaaacc aacttccttt tcactggttc caccctattt
     1081 cagttttttc actgacgtca cttcagtaag aaaccaatct atgagacggc tgagaaagaa
     1141 cctctgaaga tcgaagatgc ggttttctgt tgattcggtt aactgaatag tagggctctt
     1201 tgctctgcta tctagagttg cctccaaagc aggaggtccg ctttcaatcc tattttcctc
     1261 cgatcaggtg tacgagcagg tatccggaga acaagccttc agggatttcg atggaaacgt
     1321 ttccaccagc ttggctaaac tttgcggtcc tttctccgct gaactcaggc gagtagagct
     1381 tgatctcctc cacagtgccc aagtcctcca cctgtacatt gaggagcaac tgtccgagtt
     1441 tctccccttc ttcggagaag ggtaaaacct tgtccgtcct agtcacgtag ccctcatctt
     1501 caggaaggat gccggtggtg tttactagat ggactacata accctcttca accttaaaga
     1561 ggctgccgca aacgtcgatg ttggaaacgg acagagcttt tttctccacc aaagcgttga
     1621 gcagtctgcc gccgtcttcc ctaatcctct tgaccaggga gccggggtag taagctccat
//
LOCUS       DAOSDA010000357         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_48544_length_1673_cov_2.687268, whole genome shotgun sequence.
ACCESSION   DAOSDA010000357 DAOSDA010000000
VERSION     DAOSDA010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48544_length_1673_cov_2.687268"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PLW82_07760"
     CDS             <1..757
                     /locus_tag="PLW82_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004826140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOJ47207.1"
                     /translation="DSHCHLEDEVFDTDRDQVYEDAVANNVDVIILAGSDLDTSKKIV
                     ELTKKYPGVYGIVGVHPHEAKDAPEGYLEVLRELVQEAKILGIGEIGLDYYYDHSPRE
                     EQMRVFREQLELAKELDKPFAIHSREAALDTIDTLKEVDYHNGIFHCYSYSVEVLREI
                     VKLGYSISLGGAVTFKNAKKPVEVVREVPLDRLLLETDAPYLTPVPFRGKRNEPKLVF
                     HVAEKIAEIKGLTIEEVAMATFANTERFFNYKF"
     gene            931..>1673
                     /locus_tag="PLW82_07765"
     CDS             931..>1673
                     /locus_tag="PLW82_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOJ47208.1"
                     /translation="MRALWQGLLSFGLINIPVKMFKATESNDFHLTLLHDACHSPIKY
                     QKHCPVCGQDVKNENIVKGYEYEKGRYVLLTEEELEKAASPRSKTIEILAFIQKGEVP
                     LPYFDDTYYLHPAPGGEKAFSLLGQALKAQNKDAFCRIVLRTKPKLCLVGFSSGVMML
                     NTIHYASEVRSPEMLGTLPIYQVTAAELELAELLINKLTKPFNPEEYPDTYREAIGEL
                     VQRKLSGQEVHVAQKAPEPQVASILEALQ"
BASE COUNT          490 a          385 c          401 g          397 t
ORIGIN      
        1 agacagtcac tgtcacctgg aagatgaggt tttcgataca gatcgcgatc aggtctatga
       61 agacgcagtt gcaaataacg tagacgtgat cattcttgct ggttcagacc tcgatacttc
      121 caagaaaatc gtggagctta caaagaaata cccaggggtg tacggcatcg taggcgtaca
      181 cccccatgaa gctaaagacg cacctgaagg ctatctggag gttctacgag agcttgttca
      241 agaagctaag atcttgggaa ttggcgaaat cggcctcgac tactattacg accactcccc
      301 tcgagaggag cagatgcggg tatttcggga gcagctagag ctggcaaaag aattagacaa
      361 gccttttgct atccacagca gagaagcagc gcttgacacc atcgacaccc tgaaagaggt
      421 agactatcac aacggtatct tccactgcta ttcctacagc gtggaagtgc taagagagat
      481 agtgaagctt ggctattcga tctctcttgg tggtgctgta acctttaaga atgcaaaaaa
      541 acctgtggaa gtcgtcagag aagtaccttt ggataggctc ctgttagaaa cagatgctcc
      601 atatcttact cctgtaccat ttcgcggaaa aagaaacgaa ccgaagttag tctttcacgt
      661 tgctgagaaa atcgcagaga tcaaaggttt gacaattgaa gaagtggcga tggccacatt
      721 tgccaacaca gaaaggttct tcaattacaa gttttaaggg acttacagat caaggccgct
      781 tgcttatcgc aagcggcctt attgctactt aggtacaaga gagtaaaaga aggtttccca
      841 gttaagttga ttttctggat gcttggtgaa aaaacgtgcg gcataaagct ccccctgaag
      901 atgcatcctc aaaagagaag gggtgttttt atgcgagcgc tatggcaggg tctcttgtct
      961 tttggcctga ttaacatacc ggtgaagatg tttaaagcta cagaaagcaa cgatttccat
     1021 ctgacgcttc ttcacgatgc ttgccacagt cccattaagt atcagaagca ctgcccggtc
     1081 tgtggccagg atgtaaagaa cgaaaacatc gtcaagggat atgaatacga aaaaggccgc
     1141 tacgttctgc taaccgagga agaactggaa aaggcagctt ctcctaggtc gaaaaccatc
     1201 gaaatcctgg cctttattca gaaaggagaa gtgcctcttc cctacttcga cgacacttac
     1261 tacctccacc ctgcgccagg tggtgaaaaa gctttttccc ttttgggaca agctctaaag
     1321 gcccagaaca aagacgcctt ttgcagaatc gtgctgcgaa ccaaacctaa actgtgtctt
     1381 gtgggttttt ccagcggtgt tatgatgcta aacaccattc actatgcgtc ggaagtgcgt
     1441 tcacctgaaa tgttggggac tcttcctata taccaagtaa ctgcagccga actggaactt
     1501 gcagaacttc taatcaacaa actcaccaag cccttcaatc ccgaggaata tccagacaca
     1561 tatcgcgagg ccataggtga actagtgcaa aggaagctct cagggcagga ggttcatgtt
     1621 gcccagaaag cgccggagcc gcaggttgca agcattctag aagctctgca aaa
//
LOCUS       DAOSDA010000358         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_49268_length_1655_cov_3.565000, whole genome shotgun sequence.
ACCESSION   DAOSDA010000358 DAOSDA010000000
VERSION     DAOSDA010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49268_length_1655_cov_3.565000"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLW82_07770"
     CDS             complement(<1..381)
                     /locus_tag="PLW82_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOJ47209.1"
                     /translation="MLFIPAIDIRKGKAVRLFQGDYNQETIYSENPLELAEAFADSGA
                     RRIHVVDLDAAKGDGSNREIICQLAKKTSAVIDTGGGVRTESDIEELLGAGVSRVVLG
                     TVALENPQLVQWAVANYPGQITVGI"
     gene            complement(396..977)
                     /gene="hisB"
                     /locus_tag="PLW82_07775"
     CDS             complement(396..977)
                     /gene="hisB"
                     /locus_tag="PLW82_07775"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOJ47210.1"
                     /translation="MRRAEYSRMSEETKISCMLNLDGTGKAEVKTGVGFLNHLLTLFT
                     FHSGFDLTLNAEGDIYVDDHHTVEDVGIVLGTALDKALGDRLGINRYGFFLLPMDETL
                     ARVVCDISGRPYLVLEAAFTREMIGDLATENLKELLKSLANKAGLTLHIDILRGENNH
                     HMAEAIFKALGRALGEAVKVTSDRNPSTKGLID"
     gene            complement(974..>1655)
                     /gene="hisD"
                     /locus_tag="PLW82_07780"
     CDS             complement(974..>1655)
                     /gene="hisD"
                     /locus_tag="PLW82_07780"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOJ47211.1"
                     /translation="RVDVIAGPGNLFVTLAKKEVYGLVGIDMLAGPSEILVIADQTAD
                     PVLVASDLLSQAEHDTDAAAILVTTSQKLAEAVKGELEKQLEELPRKDYAVSSLENHG
                     ALIVAKDLKEAADVANQISPEHLEVQVEDPDQVLPLLENAGAIFIGKYASEPLGDYVL
                     GPNHVLPTSGTARFSSPLSVQTFLKASSLLKVNRQGFLKVKDGAMKIAEVEGLDAHRL
                     SVYRREKA"
BASE COUNT          359 a          451 c          399 g          446 t
ORIGIN      
        1 aataccaacg gtgatctgac cgggatagtt tgccactgcc cactggacca attgggggtt
       61 ctctagagct acggtcccca agaccaccct agagactccc gctccgagca gttcctcgat
      121 atcggactct gtgcggacgc cacctccggt atcgatcact gcagaggtct ttttggccaa
      181 ctggcagatg atctccctgt ttgatccgtc tcccttagct gcatccagat ccaccacatg
      241 gatcctcctg gcaccactgt ctgcaaaggc ttcggcaagt tccagagggt tctcgctgta
      301 gatcgtttcc tggttgtagt ctccttggaa tagacgtacg gcttttcctt tcctaatgtc
      361 tatagctgga ataaagagca tggttttcca cctttctagt cgatgagtcc ctttgttgac
      421 gggtttctat ccgaagtgac cttcaccgct tcaccaagcg ctctgcctag agctttgaat
      481 atagcctctg ccatgtggtg gttgttttct ccacgcagaa tatcgatgtg cagggtaagc
      541 cccgctttgt tggctaaact cttaagcagc tcttttaggt tttctgtagc tagatctcca
      601 atcatttctc gggtaaaggc ggcctcaagc actaaataag gccttcctga gatatcgcac
      661 accactctcg ccagcgtttc gtccatggga agaaggaaaa agccatagcg gttgatgccc
      721 agcctgtctc ctagagcttt gtccagagct gtacctaaga cgatgcccac atcttcaact
      781 gtgtggtgat cgtcgacgta gatgtcgcct tcggcgttca gcgtcaagtc aaatccactg
      841 tggaaggtaa agagggtgag gagatggttc agaaagccaa ctccggtctt tacctcagct
      901 tttccggtgc cgtcgagatt cagcatgcag ctgattttcg tctcttcgga catccggctg
      961 tactctgcac gtctcatgcc ttttccctcc tgtagacaga aagtctgtgg gcgtcgaggc
     1021 cttctacttc ggcgatcttc atggctccgt ctttaacctt aaggaaaccc tgtctattta
     1081 ctttcaagag acttgaagct ttgagaaagg tctgaaccga aagaggagag ctaaatcttg
     1141 cagtgcctga agtgggaagc acgtggtttg gcccgagcac ataatcgcct aacggctcgg
     1201 acgcgtactt accgataaag atcgctccag cattctccag aagggggagc acctgatccg
     1261 gatcttcaac ctggacttcc aagtgttccg gagaaatctg attggctaca tcggcagctt
     1321 ccttcaagtc ctttgcaacg atgagagctc cgtggttttc cagggaagat accgcatagt
     1381 cttttcttgg caactcctcc agctgcttct ccagttcccc tttaacagct tcggcaagct
     1441 tttgagaggt agtaaccagg attgctgcgg catctgtgtc gtgctctgct tgagaaagca
     1501 ggtctgaggc aaccagcacc ggatctgctg tttgatctgc aataactaag atctcgctcg
     1561 gtcctgccag catgtcgatg cctacaagcc cgtaaacttc tttcttagcc agcgttacaa
     1621 acaagttgcc cggtccggca ataacgtcca ctcgg
//
LOCUS       DAOSDA010000359         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_49562_length_1648_cov_2.672316, whole genome shotgun sequence.
ACCESSION   DAOSDA010000359 DAOSDA010000000
VERSION     DAOSDA010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49562_length_1648_cov_2.672316"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PLW82_07785"
     CDS             complement(<1..976)
                     /locus_tag="PLW82_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOJ47212.1"
                     /translation="MYLASEKATFSAVAETVSILLFAIPYIDTCQINNIILKRLNPFK
                     HAFRPILQLAGDKLLQEKKAVGDVMTEGEKLATFCEGHEIQNSWGSFWLFSYDLNLNV
                     PAEQDINVLEVFLQAVRGIGPVREKKFRAFGIQTLWDLAKKSPDGLVREDAQRVCEAL
                     EIRDWALLLERFPKFKVLSAVPREKLAVIDIETSGFTPALPIFLVGVLTFEPNPKKRI
                     FLARHPVEEPAMLKEAVSVIADKACVITYNGASFDLPMLEKRCNRLDVPFQKPFNVDL
                     LPIVRKLIGQEVPDCKLKTIERIVLQYERYNDPASSEVPEIYQRFFLER"
     gene            1144..>1648
                     /locus_tag="PLW82_07790"
     CDS             1144..>1648
                     /locus_tag="PLW82_07790"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ47213.1"
                     /translation="MRKAKWVSVVLLFVILLIFTGCGPTSLLPPTNRAPVIQGAEPGQ
                     NVTVPGGETRAFTVFATDPDGDSLSYSWSKTAGNWASGVASNTVSWTAPETGGTADIT
                     VTVSDGKLTTKHTWKVTIEEVDPNVIEIEDNIDSTTIFETGKVYIMKNFVNVNAQLTI
                     EPGAIVKF"
BASE COUNT          420 a          397 c          392 g          439 t
ORIGIN      
        1 tccgctcgag gaaaaacctc tggtagatct cagggacctc gctagatgcg gggtcgttgt
       61 atctttcgta ctggaggacg attctttcga tggtcttcag cttgcagtca ggcacttcct
      121 gtccgatcag tttccttaca attggcagaa gatccacgtt aaacggcttt tgaaaaggta
      181 catccaacct gttgcagcgc ttctcgagca tcggtagatc gaaggacgca ccattgtatg
      241 ttataacgca ggctttatct gcaatcaccg atactgcttc tttcagcatg gcaggctctt
      301 ccactgggtg acgggctaag aagatccttt ttttgggatt tggttcaaag gttaaaacac
      361 ccacgagaaa aatcgggaga gctggcgtaa atcccgacgt ttcaatatcg attacggcga
      421 gtttctctct gggaactgca gagagtactt tgaatttggg aaaacgctcg agcaaaaggg
      481 cccaatccct tatctccaag gcctcacaga ctctttgagc atcttctctt acgagtccat
      541 ctgggctttt tttcgctaga tcccacagcg tctgaatgcc aaaagcccgg aatttctttt
      601 cccggacggg gccgatgccg cggaccgcct gtaaaaaaac ctccagcacg ttaatgtcct
      661 gttcagcagg aacgtttagg tttaaatcgt aggaaaaaag ccaaaaactg ccccagctgt
      721 tttgtatctc gtgcccttca caaaaggtag caagtttttc tccttcagtc ataacatcac
      781 ctacagcttt cttctcttgt aagagcttat ctcctgccag ctgtaatatg ggacgaaaag
      841 catgtttaaa aggatttagt ctttttagta taatgttatt gatctgacaa gtgtcaatat
      901 acggaatagc gaaaagcaag attgataccg tttcggcaac tgccgagaac gtcgcttttt
      961 ccgaggccaa atacatattt ttcctcttct gggtgcacct tatagtcaga gtggtgcagt
     1021 ttcaaaacgt ctaccggctt cgtctttttt cttcgtgagc tgcatgctga tgcagattgg
     1081 ccgcggaaaa agcgtgcgca agccgctttc gtttgcctac actaaaccta aaggaggaag
     1141 ttcatgagaa aagcaaaatg ggtctctgta gtactgctgt ttgtgattct tctgatcttt
     1201 acaggctgtg gaccaacatc attgctcccg ccgacaaaca gggctccggt aattcagggc
     1261 gcagagcccg gccaaaatgt aacggttccc ggtggggaaa cgcgagcttt tacagtcttc
     1321 gcgacagatc ccgatggcga ctcgctgtcg tattcctgga gtaagacagc aggcaattgg
     1381 gcctcgggtg ttgcaagcaa cacggtctct tggaccgctc ctgagaccgg cggtacggcc
     1441 gacatcaccg ttacagtctc agatggcaag ttaacgacta aacatacctg gaaggtaacc
     1501 attgaggaag ttgatcccaa tgtaatcgaa atcgaggaca acattgactc aaccactatc
     1561 tttgagaccg gtaaggtcta catcatgaag aactttgtta atgtaaatgc gcagctgacc
     1621 atcgagccag gtgccatcgt gaagttcg
//
LOCUS       DAOSDA010000360         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_49754_length_1643_cov_4.311083, whole genome shotgun sequence.
ACCESSION   DAOSDA010000360 DAOSDA010000000
VERSION     DAOSDA010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49754_length_1643_cov_4.311083"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..655)
                     /locus_tag="PLW82_07795"
     CDS             complement(41..655)
                     /locus_tag="PLW82_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOJ47214.1"
                     /translation="MEISREVLDKRGIYWENRIKVFLQEEVPENPIVFIGDSLTERFP
                     LEKYFGKGFVNRGIGGDHADGLWERKSLLCLDKNPRALFFMIGINDLLFNYKRDEIPY
                     HLDRIITYAKEKAPSAKIVIQSICPVRNWEKPTPDELNKINGELQDLAVKHGASFLNL
                     FELFADENQFLKEELTIDGVHFSEAAYDIWAEEVRKWLKQQDLG"
     gene            complement(690..1091)
                     /locus_tag="PLW82_07800"
     CDS             complement(690..1091)
                     /locus_tag="PLW82_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HOJ47215.1"
                     /translation="MGFGPTFMLFETVFPILFIVVFLLVLGAFVYTIGYSVFRGIKNQ
                     KSPELVRTAKVVSKRTEVGSYGSHRHHHQTHHMHTGVRTYTRYFVTFELEDRSREEFQ
                     VTDRDYGLLVEGDEGRLFSRGTRFQGFERYR"
     gene            1336..>1643
                     /locus_tag="PLW82_07805"
     CDS             1336..>1643
                     /locus_tag="PLW82_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOJ47216.1"
                     /translation="MIVVSNVKSPKVVLALDSFKGSLSSHEAVAAAAAGVRRVLQDAE
                     ILKIPVADGGEGTAQVLAEAASGSMHQVTCKDPLFRDIQCSFGLSKDRETAFIEVAAS
                     "
BASE COUNT          427 a          442 c          346 g          428 t
ORIGIN      
        1 cgaatacttt ttcaagtgcc aaaatcccct gccttcccct ttagccaaga tcctgctgct
       61 tcagccactt tcttacctct tcagcccaaa tatcgtaagc ggcttcggaa aaatgcacgc
      121 cgtcgatggt aagctcttcc tttaggaact ggttctcatc tgcgaacagt tcaaagaggt
      181 taaggaaaga agctccgtgc tttacagcta ggtcttgcaa ctctccgtta atcttgttca
      241 gctcgtcggg cgttggtttt tcccaattcc tcacagggca gatcgactga atgactatct
      301 ttgcgcttgg agctttctcc ttggcgtaag tgatgattct atcgagatga tagggaattt
      361 catccctctt gtagttgaat aaaagatcgt tgataccgat cataaaaaac aacgctcttg
      421 ggtttttgtc caagcacaaa agactttttc tttcccacaa tccatcggca tggtctccgc
      481 caatcccccg gttgacaaaa cctttgccga agtacttctc cagcggaaac ctctcagtta
      541 gtgaatcgcc gatgaaaact atcgggttct ccggtacttc ctcctgaaga aaaaccttaa
      601 tgcggttttc ccaatagatg cctcttttgt ccagtacttc tctagaaatt tccatataga
      661 caactccttc tactacttta ggtatggttt catctatacc tctcaaatcc ttggaaacgc
      721 gtccctctag aaaacagacg tccttcgtcc ccctctacca gaagtccgta gtctctgtcg
      781 gtcacttgaa actcctccct agaccggtct tccagctcaa aggtgacaaa atacctggtg
      841 tatgtacgca caccggtatg catgtgatgg gtctggtggt gatgcctatg agacccgtag
      901 gagcctactt ccgttctctt ggaaaccact ttggcagttc tcacaagttc cggactcttt
      961 tggtttttta ttcccctaaa gacgctgtac cctatagtgt agacaaacgc tcccaggacc
     1021 agtaaaaaca ctacgataaa caggatcgga aaaaccgttt cgaaaagcat aaaagtcggt
     1081 ccaaaaccca aaaagaaaac ctcctctcgc atcgcctggt acaaaggcta gagattttcg
     1141 tgaaacctca tgcatccgtc cactatcccg ctttaccttc ctcagttcaa cagcaaaaac
     1201 ccaaatcctg cctgctagct ctggcaaagc taagaccttt gacagcagtc caaaagcccc
     1261 ttttgcccgt tttacaaagg tctgaaggaa agcagtggta cacaggaaaa tatagaagta
     1321 tcacgctttt tgggggtgat cgtcgtaagc aacgttaaaa gcccaaaagt ggttctcgca
     1381 ctggactcgt tcaaagggag cctctccagt cacgaggcgg ttgcagctgc tgccgcagga
     1441 gtccgaagag tgctgcagga cgccgagatc ctgaagatcc ccgtagcaga tggaggcgag
     1501 ggcacagcac aggtcctcgc agaagctgcg tccggttcca tgcaccaggt aacctgcaaa
     1561 gatccacttt tcagagacat ccaatgctct ttcgggctct ctaaagacag ggaaaccgct
     1621 ttcatcgaag ttgcggcaag tag
//
LOCUS       DAOSDA010000361         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_51255_length_1612_cov_2.447013, whole genome shotgun sequence.
ACCESSION   DAOSDA010000361 DAOSDA010000000
VERSION     DAOSDA010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51255_length_1612_cov_2.447013"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLW82_07810"
     CDS             <1..395
                     /locus_tag="PLW82_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpC"
                     /protein_id="HOJ47217.1"
                     /translation="ESSAYESMKDKVMGQLKKTFRPEFLNRVDEIMVFHALSKGEIKK
                     IVELMIGELQERLKEQGLEIEATEKAKNVLVEEGFDPDFGARPLRRAIQRLVENPLSD
                     EILAGKFKNGDKVLVDADENNKIIFNKE"
     gene            563..>1612
                     /gene="radA"
                     /locus_tag="PLW82_07815"
     CDS             563..>1612
                     /gene="radA"
                     /locus_tag="PLW82_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOJ47218.1"
                     /translation="MAKAKSLYVCSACGAQSYKWQGKCTNCDSWNTLELQEAARKSQD
                     RGFSLRSSSPPTPISEVSSQQEERYQTGIAELDRVLGGGVVPGSLTLLAGEPGVGKST
                     LLLQMAKEFSEKWGKVLYISGEESLQQIKMRAKRIGAANNSIYLLSENNVDSMIRAIE
                     DIDPAIVIVDSIQTAYVPEQESAPGTIAQVRESTAQLLRLAKGENVPIFLVGHVTKTG
                     VAAGPQVLMHMVDTVLNFTGDKFSLYRILASEKNRFGATDEIGIFEMKNTGLSEVHNP
                     ARLFLSGRKTDEPGTAIVPALEGTRPILVEIQALVTKGYGAMPRRQFSGTDRNRTPIL
                     LAVLEKRLGLPIGNYD"
BASE COUNT          449 a          390 c          432 g          341 t
ORIGIN      
        1 ccgaatccag tgcgtacgag tccatgaagg ataaagtcat gggccagctg aaaaagacct
       61 tcaggccgga gttcctcaac agggtcgacg agatcatggt cttccacgct ctgagtaaag
      121 gggagatcaa gaagatcgtc gagctgatga tcggcgagct ccaggaaagg ctcaaagagc
      181 aggggctgga gattgaagct acagaaaaag caaagaacgt ccttgtagaa gaaggctttg
      241 atccggactt cggtgcaaga ccgctgagac gcgctattca acgtctggtg gagaacccgc
      301 tcagcgatga gattcttgca ggcaagttca aaaatggcga taaagtcctt gtcgatgccg
      361 acgaaaacaa caagatcatt ttcaataaag agtaggagcc tgcggtgcgg cagaaagctg
      421 tcgtaccgca gtttttctac gccttgtacc cacctgaaat aaacctggag ttctctgaag
      481 cttcttggag gattataaac atgcattatg gaaggttata aaatctaggt gattatacgc
      541 tacaggtcga ggagggtgga aggtggccaa ggcaaagtct ctttatgtct gctctgcctg
      601 cggagcccaa agctacaagt ggcaggggaa atgcacaaac tgtgatagtt ggaacactct
      661 ggaactacag gaagctgcaa gaaaaagtca agacagaggc ttcagcctcc gcagttcctc
      721 tccgcctacg cccatcagtg aagttagctc ccagcaagaa gagcgctatc agaccgggat
      781 tgccgagctc gatagggtcc tcggaggtgg agtagtaccc ggctcattga ccctgcttgc
      841 aggagaacct ggagtaggaa aatcgaccct acttctccag atggccaagg aattttctga
      901 aaagtggggc aaagttttat atatcagcgg tgaagagtcc ctgcagcaga ttaagatgcg
      961 ggcaaagcgt atcggtgccg ctaacaacag tatctacctt ctcagcgaga acaacgtgga
     1021 tagcatgatc cgcgccatcg aggacatcga tcctgctatt gtcatcgtgg actccatcca
     1081 aactgcttat gttcccgagc aggaatcggc accaggaact atcgcccagg tgagagagtc
     1141 cacagcccag ctcctcaggc tggccaaagg cgaaaacgtt cccatcttcc tcgttggtca
     1201 cgttaccaaa accggggttg ctgccggtcc tcaagtactg atgcatatgg tggatacagt
     1261 gctgaacttc acaggggaca agttcagctt gtaccgcatt ttagcgtcgg aaaagaaccg
     1321 cttcggtgct accgacgaaa taggcatctt tgaaatgaaa aacaccggtc tgtcagaagt
     1381 ccacaatccc gcacgactgt ttctcagtgg cagaaaaacc gatgagcccg gtacggctat
     1441 cgtacctgct ctagagggga caagaccgat cttagttgag attcaagcgc tagtaaccaa
     1501 gggctacggt gcgatgccca gaagacagtt ttccggtaca gaccggaacc gcacaccaat
     1561 tcttcttgcg gttttagaaa agcgtttggg actgccaatc ggcaactacg at
//
LOCUS       DAOSDA010000362         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_51595_length_1605_cov_3.228387, whole genome shotgun sequence.
ACCESSION   DAOSDA010000362 DAOSDA010000000
VERSION     DAOSDA010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51595_length_1605_cov_3.228387"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..836
                     /locus_tag="PLW82_07820"
     CDS             177..836
                     /locus_tag="PLW82_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47219.1"
                     /translation="MDELIHLSVDTKIEQATSQVFPEMNQQIIDSLCREINSITDYFR
                     SVLNENTLYVSDEWTGERFKQDFLMTSVHAVRQGRQSEIWPNLKKTSGDLIIDCLMDM
                     IESAKTSMSPDEVVSKGLVPKVIGVDAATEILHKFYPTKFATRNERLEAGLAYFLFDG
                     DKDRVKSLTYEEYIKYMKDLAEKLNEHYSARDLSISETCKLFLLEKLLGRVVDVSSDQ
                     E"
     gene            936..>1605
                     /locus_tag="PLW82_07825"
     CDS             936..>1605
                     /locus_tag="PLW82_07825"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOJ47220.1"
                     /translation="MKKSNLYNGLWALFSVVMVVLLVRYPEQALQAAIEGAAVWWEAV
                     FPALLPFFIVSEIALGTGVVNLLGALLEPLMRPVFNVPGEGAYSVAMGIASGYPLGAK
                     ITRGLYEAGDLTKGEAERLVSFSSTANPVFIVGAVAVGMFKSPHAGAAMALVHYTSAL
                     LLGFFLSFLEKRPKDKGRPRRKATLHEAWERMINARRKDGRSFGEILGDAVWQSVNSA
                     LKIGG"
BASE COUNT          410 a          342 c          437 g          416 t
ORIGIN      
        1 cgctctgtac gtagaaaaag cactaaaaac cagcaatgtt caaaacacgc ccggcgagat
       61 cagtggctat gttgttgcgg atattctcga acgcagtatc agctgaagca aggatactca
      121 catgggcatc aatatggctt atgttcgagc tttagattgg aaggggagct tactagatgg
      181 acgagttgat tcatctatct gtagatacaa aaatagagca ggcaacgtct caagtctttc
      241 cagagatgaa ccagcagata attgacagtt tatgcagaga aataaactcc attaccgatt
      301 acttccgcag tgtgcttaat gaaaatactt tgtacgtttc cgatgagtgg accggggaaa
      361 gattcaagca ggactttctt atgacttcgg ttcatgcagt aaggcagggc agacaatctg
      421 agatttggcc caacctgaaa aagacttctg gggaccttat tatcgattgc cttatggaca
      481 tgattgagtc tgcgaaaact tcgatgagtc ccgatgaggt cgtttctaag ggattggttc
      541 ctaaagtgat cggggttgat gcggccacag aaatcctgca caagttttat cctacgaagt
      601 ttgccacgcg caacgaaagg cttgaggcag gcttggccta cttcctgttc gatggagata
      661 aggacagggt aaagagcctc acttatgaag agtacatcaa gtacatgaag gatcttgccg
      721 aaaagctgaa cgaacactac agtgcccgcg atctatcgat atccgagacg tgcaagctgt
      781 ttttgttgga gaagctgctc gggcgtgttg tggatgtttc aagcgatcaa gaatgatttc
      841 atagagtttt cctgccgcag gcatgccctg cggtttttct cttcctaaag gtctagtttg
      901 ggctcacagg ccataaattg gttaggggtg gtgctatgaa aaaatccaac ctctacaacg
      961 ggctatgggc ccttttcagc gtagttatgg tcgttcttct cgtgaggtat cccgaacaag
     1021 ccctgcaggc cgcgatagaa ggagccgcgg tatggtggga agcagttttt cccgcccttt
     1081 tgccattttt cattgtttcg gagattgcct tgggcacagg cgtggtgaat ctcttaggtg
     1141 ctcttttaga accgttgatg aggcctgtgt tcaatgttcc cggtgaggga gcttattcag
     1201 tggctatggg tatcgcttcc ggatatcctt taggtgcaaa aatcacccgt ggcttgtacg
     1261 aagctgggga tctcacaaaa ggggaagcgg aaagactggt cagcttcagc agcaccgcaa
     1321 acccggtgtt tattgtcggt gcggtagctg ttgggatgtt caagagcccg catgcaggtg
     1381 cagcgatggc gctagtacac tacacctcag cgcttctttt gggttttttc ctcagctttt
     1441 tggaaaaacg gccaaaggac aaagggcgtc ctagaagaaa agccactctg catgaggctt
     1501 gggagaggat gatcaatgcc agacgcaaag acggacgctc ttttggcgag attctcggcg
     1561 atgcagtctg gcagagcgtc aacagcgcac tgaagatagg aggtt
//
LOCUS       DAOSDA010000363         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_52231_length_1592_cov_2.753416, whole genome shotgun sequence.
ACCESSION   DAOSDA010000363 DAOSDA010000000
VERSION     DAOSDA010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52231_length_1592_cov_2.753416"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /gene="lepA"
                     /locus_tag="PLW82_07830"
     CDS             <1..1494
                     /gene="lepA"
                     /locus_tag="PLW82_07830"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOJ47221.1"
                     /translation="VVDATQGIEAQTLANLYQALDHDLKIIPVINKIDLPSAQVDEVT
                     AEIEEILGETEVYPVSAKTGEGVLEVLEAIVREIPPPKGDPDAPLRALIFDSYFDSYR
                     GAVAIFRVVDGKVKVGDRIMMYSTGKVFEVTELGTMIPTQVPLESLVSGEVGYLTASM
                     KDVKDTSVGDTITLADNPAKEPLPGYRKAKPMVFCGLYPVDNDQFPDLRAALEKLQLN
                     DASLVFEPETSAALGFGFRCGFLGLLHMDIVQERLEREYNLNLIVTAPSVIYHVVTTK
                     GNLLEIDNPSKLPSAQEIDEIHEPMVQATIITPDTYVGPIMQLCQDKRGMFKNMEYLN
                     PTRAMLTYEIPLSEILMDFFDRLKSCSQGYASLDYEITKYQQADLVKLDILINGKPVD
                     ALSTIIHREKAAHYGRSLAKKLKEVIPQHLFEVPIQAAIGSKVVARETVRALRKDVLA
                     KCYGGDVSRKRKLIEKQKEGKKRMKAIGNVEIPQEAFMSVLKVDDND"
BASE COUNT          426 a          401 c          401 g          364 t
ORIGIN      
        1 gttgtagatg ctacccaggg catagaagcc cagacccttg ccaatttgta ccaagctttg
       61 gatcacgacc tgaagatcat accggttatc aataaaatcg atcttcccag tgctcaggtg
      121 gatgaagtaa cagcggagat cgaagaaatc ctcggcgaaa ccgaggttta ccccgtaagc
      181 gctaagaccg gcgaaggggt cctcgaagtt ttggaggcga ttgtaaggga aattcctcct
      241 ccaaagggag acccagatgc accgctgagg gcgctgattt ttgactccta cttcgactcc
      301 taccggggtg cagttgcgat tttccgggta gtagacggca aggtcaaagt gggagacagg
      361 atcatgatgt actctaccgg caaggtcttt gaggtcaccg agctaggcac tatgatacca
      421 acgcaggtgc cgcttgaaag ccttgtaagc ggtgaggtag gctacttgac ggcctctatg
      481 aaagacgtca aggacaccag tgtgggcgat accatcacct tggcggacaa ccccgccaaa
      541 gaaccacttc ctggataccg caaggccaaa ccgatggttt tctgtgggct ttaccctgta
      601 gataacgacc agttcccaga tctcagggct gctctagaaa agctccagct gaacgatgcc
      661 tctctggttt ttgaaccgga gacctctgca gcgctggggt tcggttttag gtgcggtttc
      721 ttggggcttc tgcacatgga cattgtccag gagaggctgg agcgggagta caatctcaac
      781 ctgatagtca cagcaccgtc ggtaatctac catgtggtta ctactaaggg caatcttctc
      841 gagattgaca atccttccaa actcccgtct gctcaggaga ttgacgaaat tcacgagccc
      901 atggtgcagg caaccattat cacgcccgat acctacgtcg gaccgatcat gcagctctgc
      961 caggacaagc ggggcatgtt caagaatatg gagtacctca atccaaccag agcgatgctc
     1021 acctacgaaa ttcctctttc agagattctc atggactttt tcgatcgctt aaagtcctgc
     1081 agccaaggtt atgccagttt ggactatgag ataaccaagt accaacaagc agacttggta
     1141 aaactagata ttttaatcaa cggcaaacct gtagatgctc tctcgaccat catccacagg
     1201 gagaaagccg ctcactacgg caggtctttg gccaaaaagc tcaaagaagt cattccacag
     1261 cacctgttcg aagtgccgat tcaggctgct attggttcaa aagtcgttgc cagagaaact
     1321 gtgcgggcgc tccggaaaga cgtccttgcc aagtgctatg gcggcgacgt ttcccgcaag
     1381 cgcaagctca ttgaaaagca aaaagaaggt aaaaaacgca tgaaggccat tggcaacgtt
     1441 gaaatacccc aagaggcatt catgtcggtt ctgaaagttg atgacaatga ttaaagcagg
     1501 tgtctacgtc cacatacctt tttgtagaaa aaaatgcgct tactgcgact ttctttctca
     1561 cgttccaaca ccgctggaga tcgagcggta ta
//
LOCUS       DAOSDA010000364         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_52899_length_1578_cov_3.175312, whole genome shotgun sequence.
ACCESSION   DAOSDA010000364 DAOSDA010000000
VERSION     DAOSDA010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52899_length_1578_cov_3.175312"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLW82_07835"
     CDS             <1..188
                     /locus_tag="PLW82_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47222.1"
                     /translation="TSTKALFSGQSLARNDVASGFQGLKPDQKVKVYTHDRIFAGIYR
                     FQDSNEPRLIPEKVFIS"
     gene            206..1126
                     /gene="ribF"
                     /locus_tag="PLW82_07840"
     CDS             206..1126
                     /gene="ribF"
                     /locus_tag="PLW82_07840"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOJ47223.1"
                     /translation="MEILDLDGAKKERTVIVLGAFDGLHIGHVSLLEQGRTLAKKKRA
                     KLAIMTFAQHPSAVLKKRSVKLLTPGQEKLDILADLGVEVVFLPDFTKIARFSPMDFF
                     YDILLGKFGAVGLVAGPNFRFGKGAKGDSNLLEYLGRKSQVPVRIGKAVTYRNGTVSS
                     TMIRRLLREGRIEVANRLLGRPFRLTGEVVRGEGRGRKLKMPTANIQLPEAKFLPACG
                     VYLVRAFAENKSYYGLLSISDKPTFHESADIAVEVYLFDTDDDLYGKTLSIEIIRYQR
                     GIVKYESAKELMDQVSRDIQEARGYLAHYL"
     gene            1315..>1578
                     /gene="rpsO"
                     /locus_tag="PLW82_07845"
     CDS             1315..>1578
                     /gene="rpsO"
                     /locus_tag="PLW82_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOJ47224.1"
                     /translation="MALDSQEKRAIIEKYQTHEGDTGSPEVQIAILSKRISDLTEHLQ
                     SHPKDFHSRRGLYKLIGQRNGLLRYLRDKDIERYRTLIENLGIR"
BASE COUNT          453 a          345 c          421 g          359 t
ORIGIN      
        1 aaacgagcac gaaggctctt ttcagcgggc aaagcctagc ccgaaacgat gtggcaagcg
       61 gatttcaagg actgaagcca gatcaaaagg ttaaagtata cactcacgac aggatatttg
      121 ccggcatcta cagatttcaa gacagcaatg aacccaggtt aataccggaa aaggtgttta
      181 tttcctgagc tttggcggtg gttaaatgga aatactggat ttagatggtg caaaaaaaga
      241 acgtaccgta atcgtgctag gagcctttga tggactgcac ataggtcatg tgagtttgct
      301 cgaacagggt aggactttgg cgaagaaaaa gagggcaaag cttgccatca tgacttttgc
      361 ccagcatcca agcgcagtgc tgaagaagcg ctcggtaaag ctgctcactc ccgggcagga
      421 gaagctggac attttggcgg accttggagt tgaggtagtc tttctgcccg actttaccaa
      481 aatagctcgt ttcagcccta tggacttttt ttatgacata ctattgggca agtttggtgc
      541 agttggactg gttgcaggcc ccaacttccg cttcgggaaa ggcgccaaag gcgacagcaa
      601 cctcttggag tatttgggaa gaaagagcca ggttccggtt cgcattggca aagcggtgac
      661 ctatcgaaac ggcactgtat cctccacgat gatccgcagg cttctgcggg aaggacggat
      721 cgaagtggca aaccgtcttt tgggaaggcc cttcaggctg acaggcgagg tggtcagagg
      781 cgaaggtagg ggcagaaagc tgaagatgcc tacagccaac atccagctgc ctgaggcaaa
      841 gttcctccca gcgtgcggag tgtaccttgt gagggctttc gcagaaaaca agtcgtacta
      901 cggcctgctt tccatatcgg acaaaccaac atttcacgaa agcgcagaca ttgcagttga
      961 ggtttatctt ttcgacaccg acgacgacct ctatggcaag accctttcca tagagatcat
     1021 ccgctatcag agagggattg ttaagtacga aagcgcaaaa gaactgatgg accaagtaag
     1081 ccgggacatc caagaggcca gaggctatct tgcccattat ctgtagaaag ctgcctggtc
     1141 ggggaagtac ggctccacag catgccgacc ggtgaaggta tatcttttgt agaaatattg
     1201 ttaatcacta gcttgtgtgg taaactaagt ggagaagcaa cccaatgctc agcaaaacga
     1261 aacctccgac gtttagcttg gctttggggg atatatgaat aggaggtgga actaatggca
     1321 ttagattcac aagaaaagag agctatcatc gagaagtatc aaactcatga aggtgatact
     1381 ggttctcctg aggtgcagat cgcgatcttg agcaaaagaa tcagcgacct gaccgagcat
     1441 ctccagagtc acccaaagga tttccattct cgccgtggct tgtacaagct cattggtcaa
     1501 agaaatggtc tcctgagata cctgagagat aaagatattg aaagatatag aactctcatc
     1561 gaaaacttag gtattaga
//
LOCUS       DAOSDA010000365         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_53990_length_1556_cov_2.966689, whole genome shotgun sequence.
ACCESSION   DAOSDA010000365 DAOSDA010000000
VERSION     DAOSDA010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53990_length_1556_cov_2.966689"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..801)
                     /gene="pyrH"
                     /locus_tag="PLW82_07850"
     CDS             complement(79..801)
                     /gene="pyrH"
                     /locus_tag="PLW82_07850"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011230751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOJ47225.1"
                     /translation="MEPKYRRVLLKLSGEALAGEQGYGLDPAVLHRISQEIKELTESG
                     VQVALVVGGGNIWRGARGSAKGMDRAQADYMGMLATVINALGLQDALEQNGVDTRCMT
                     AIEMRQIAEPYIRRRAVRHLEKGRVVIFASGTGNPYFSTDTTAALRAAEIEAEVILMA
                     KRGVDGVFSADPAVDATATKYDQIGYLDLLNKGLQVIDATAASLCMVNNIPIIVFDFN
                     KQGSALKAVMGESIGTLIGGGQ"
     gene            complement(902..>1556)
                     /gene="tsf"
                     /locus_tag="PLW82_07855"
     CDS             complement(902..>1556)
                     /gene="tsf"
                     /locus_tag="PLW82_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOJ47226.1"
                     /translation="LVSETIALIMKEKPADTAALMQLAYDGATFEEYLKSKIANTGEN
                     ITVRRFRVLEAASENENITSYIHLNGKIGVLVHAKADSADAVKSETFTELMKDLALQI
                     AAANPQVVNRNQVSAEKLEEERKIYRAQALQEGKPEKIVDKIVDGRMSKYYSEVCLLE
                     QEFIREDKMTVDAYIKQVAKSLGTNIEVLDFVRFERGEGIEKKVDDLAAEVAKMTNQ"
BASE COUNT          346 a          428 c          325 g          457 t
ORIGIN      
        1 gcaacgtgct gagctctctt ttagctgctt caacaacctt ttccattctg gctttagctt
       61 catcaagtac cttttgcatt attgtcctcc cccaatcaat gttccaattg attcacccat
      121 aacggccttc aaggcacttc cctgtttgtt aaagtcaaaa acaataatgg gaatattgtt
      181 aaccatacac agcgacgccg cagtagcgtc aatcacctgc agacctttgt tcaaaagatc
      241 aagatagccg atctgatcgt acttagttgc ggtagcatcc acagctggat ctgctgaaaa
      301 gacgccatcc acgccgcgtt tggccatgag aattacttcc gcctcgatct cggcagctcg
      361 caaagctgcc gtggtgtcgg tggaaaaata aggatttccc gttcccgaag caaaaatcac
      421 cacacgtccc ttctccaggt gccttacggc tcgcctcctg atgtagggct ctgcgatctg
      481 tctcatttcg attgcggtca tgcacctggt gtcaacgccg ttttgctcca gagcgtcctg
      541 aagacccaaa gcattgatta ctgtggcaag catgcccata tagtcagctt gagcgcgatc
      601 catgcctttg gcactgcccc gtgcgccccg ccaaatgttt cctccaccta caaccagtgc
      661 tacttgtact cccgactctg taagttcttt gatctcttga gaaatacggt gcagtacggc
      721 aggatctagt ccataaccct gctctcctgc caaagcttcg ccgctcagct tcagcagaac
      781 tcgccggtat tttggctcca tgtttgccct cctgttctct ccttgaataa ttctcttttt
      841 tagcttaaca tccttgtgta ttgcaaaaaa aggagaacac ttgaggtgct ctcctaattt
      901 attactgatt ggtcattttg gcgacctcag ctgccagatc gtcaactttt ttctcgattc
      961 cttcgccgcg ctcgaaacgc acaaagtcga gaacttcgat attcgtaccg agactcttgg
     1021 caacttgctt aatataggca tcaactgtca ttttatcctc gcggatgaat tcctgctcca
     1081 acagacagac ctcgctgtag tacttgctca tacgaccgtc aacgatcttg tcaacgattt
     1141 tctctggctt accttcttgg agagcttgag ctctatagat ctttctctct tcttcgagct
     1201 tctctgcact cacctgattg cggttcacta cctggggatt tgcagcagcg atttgcagtg
     1261 caaggtcttt catcagctcg gtgaaggttt cgctcttaac agcgtcagcg gagtcagctt
     1321 tggcatgaac cagaacgccg atcttaccgt ttaggtgaat gtacgatgtg atgttctcgt
     1381 tttccgatgc tgcctcaaga actctaaagc gacgaactgt gatgttttct ccggtgtttg
     1441 cgattttgga cttgaggtat tcctcgaaag ttgcaccatc gtaagcgagc tgcatcaaag
     1501 cagcggtatc tgctggcttc tctttcataa tgagcgcgat agtctcactc accaat
//
LOCUS       DAOSDA010000366         1552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_54186_length_1552_cov_3.218437, whole genome shotgun sequence.
ACCESSION   DAOSDA010000366 DAOSDA010000000
VERSION     DAOSDA010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54186_length_1552_cov_3.218437"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLW82_07860"
     CDS             <1..208
                     /locus_tag="PLW82_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47227.1"
                     /translation="VIVGTLSRYSPKLGGQTGTVLVCRYNNKHYANKRWQFNVYWRFL
                     LVEVVKLGRTVVFTSSIRAMRAFS"
     gene            477..806
                     /locus_tag="PLW82_07865"
     CDS             477..806
                     /locus_tag="PLW82_07865"
                     /inference="COORDINATES: protein motif:HMM:NF017894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 family protein"
                     /protein_id="HOJ47228.1"
                     /translation="MTQKTLSARVRTELPENLTEFIWQLAEAARERSRLQREEIQVFE
                     LSPINHSAIVENQMVVHIVGEGYQRSYSLCLEDSFAGKIVVKDLGDELIMMFSDEYDQ
                     EQERGED"
     gene            complement(835..>1552)
                     /locus_tag="PLW82_07870"
     CDS             complement(835..>1552)
                     /locus_tag="PLW82_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOJ47229.1"
                     /translation="GYKFGFVALTSALVNITLKNIFKSPRPVGQEGIRSLRLETAGGY
                     SFPSGHSQFAATSWTTLALHLRKRWFSILAGVLIVLVGLSRLYLGVHWPVDVVFGIAI
                     GAIWAFLGSKIIDLLEEKPVSYLVLGAALLLALPIFQNADYFKVVAACSGICIGIFLE
                     GKYVGYTVKAPLLIQLIKALLGVAVLLGIQNGLKLILPDKLISHGLRYFLIGIWATFL
                     APLCFKAIFKLKNQVEKSAS"
BASE COUNT          460 a          323 c          407 g          362 t
ORIGIN      
        1 tgtgatagtt ggaacattaa gcagatattc tccaaagttg gggggtcaaa ccggaactgt
       61 tttagtgtgt agatacaaca acaaacacta cgccaataaa cgttggcagt tcaacgttta
      121 ttggcgtttt ctactggtgg aagttgtcaa actcgggagg acggttgttt ttacaagctc
      181 gatacgcgca atgagggcct tttcttaaga aggtcggaag agagtagatg cttcgttttt
      241 tcagagacgt ggagaaaaac tgaggtatcc tttttggaat gaaaagtcga atggatgaac
      301 cagatctttt cctgacttag agtactgatg tcatttatta gtccaaaata aggttagaac
      361 gtccagatct gtggtactat ggattagtct gtgcaattgt ccgtttgaaa tcacgggtta
      421 cacacgggaa acacagcaaa cgcagtttgc atgtacctac aaggaggtcc tattcattga
      481 cacagaaaac cctatcagca cgggttagaa cggaactgcc agaaaacctc acagagttca
      541 tttggcagct ggcagaagca gctagagaac gcagcaggct tcaaagagag gaaattcagg
      601 tctttgagct tagccccatc aatcacagtg caatcgttga gaaccaaatg gtagtccaca
      661 tcgtagggga agggtaccaa cgcagctact ctctctgcct cgaagatagc tttgcaggca
      721 agatcgttgt aaaggatctt ggagacgagc tgataatgat gttctccgac gagtatgacc
      781 aggagcagga aagaggagaa gactagaggt ggcgaaaagc gcagcttaga ttggctaaga
      841 tgcgcttttc tctacttggt ttttcaattt gaagatggct ttaaagcaca aaggtgcaag
      901 gaaagtagcc cagatgccaa taaggaagta cctgagtccg tgggaaatca gcttgtcggg
      961 aaggatcagc ttcaagccgt tctgtatacc aagaagcact gcaactccca aaagggcttt
     1021 gataagttga atgagaagcg gagctttcac agtgtagcca acgtacttcc cttctaaaaa
     1081 gatgccaatg cagataccgg aacaggctgc aaccactttg aagtagtcgg cgttttgaaa
     1141 gatgggtaag gccagaagta gcgctgcccc aaggactagg taggaaacgg gtttttcttc
     1201 cagaagatca atgatcttgc tgcctaaaaa cgcccaaatt gcaccgatgg caatgccaaa
     1261 gaccacatca acaggccaat ggacgccgag gtacagccgg gaaagaccta caagaacgat
     1321 gagcacccca gctaatatgc tgaaccagcg cttacgcaga tgaagggcaa gggttgtcca
     1381 gctggttgcg gcaaactggc tgtgccctga agggaaggag taaccgcctg ctgtctccaa
     1441 acgcagagag cgaattcctt cctgtccgac gggtcttggg cttttgaaga tatttttcag
     1501 ggtgatgttg actagagcag aagtcagtgc tacaaagccg aacttgtacc cg
//
LOCUS       DAOSDA010000367         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_54876_length_1538_cov_3.613621, whole genome shotgun sequence.
ACCESSION   DAOSDA010000367 DAOSDA010000000
VERSION     DAOSDA010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54876_length_1538_cov_3.613621"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /gene="guaA"
                     /locus_tag="PLW82_07875"
     CDS             <1..848
                     /gene="guaA"
                     /locus_tag="PLW82_07875"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOJ47230.1"
                     /translation="VTALLVHKAVGDQLTCIFVDHGLMRLGEPEEVVKTFRDRFNLNI
                     VHVDASERFLALLEGVTDPEQKRKIIGMEFIRVFEEEAKKIGDAKFLAQGTIYPDVIE
                     SGIDSKGTIKSHHNVGGLPEDMKLDLVEPLRLLFKDEVREVGLELGMPENLVYRHPFP
                     GPGLAVRVIGEITKERLDILRQADAIYIDEIRKHGLYNEIWQAFCILTDTRTVGVSGG
                     ERTYAYMLGLRAVNSTDAMTAEWYPFPHDVLQKISSRILQEVPQVNRVVYDVSSKPPA
                     TIEWE"
     gene            1126..>1538
                     /locus_tag="PLW82_07880"
     CDS             1126..>1538
                     /locus_tag="PLW82_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ47231.1"
                     /translation="MEFKLESVSLQKQEKTILSSISLEIPAGSIFILIGPSGSGKSSL
                     LRLLNRLDAPTSGRILYKDQPIESYPVQELRKEVGMLFQHPVMINGTVRDNIALGHKL
                     QKKDLPEEKVLELIERVGLEKDLIDKDASVLSGGQ"
BASE COUNT          440 a          330 c          408 g          360 t
ORIGIN      
        1 cggtaactgc ccttcttgtc cacaaggctg ttggagatca gctgacctgt atctttgtag
       61 accacggatt gatgaggctt ggcgagccag aagaagttgt gaagaccttc agagaccgct
      121 tcaacttgaa tatcgtccat gttgacgctt ccgagagatt cctcgccctt cttgaaggtg
      181 tcaccgatcc cgaacagaaa cgcaagatca tcgggatgga attcattaga gtattcgaag
      241 aagaagccaa aaagatcgga gatgctaaat tcttagcaca gggtaccatc tacccagatg
      301 tgatcgaaag cggaatcgac tctaagggta cgatcaagag ccatcacaac gttggcggac
      361 tgcccgaaga catgaagctg gaccttgtgg agccactgcg cttgctcttt aaggatgaag
      421 ttcgcgaggt gggtctggaa ctgggtatgc cagaaaacct ggtctacaga catcccttcc
      481 ccggccccgg tcttgcagtg agagtcatag gcgagatcac caaagagaga ctggatatct
      541 tgcggcaggc tgacgccatt tacatcgacg aaatcaggaa acacggcctt tacaacgaaa
      601 tctggcaggc gttctgcatc ctgaccgaca cccgcactgt aggagtttcc ggtggtgagc
      661 ggacgtatgc ttatatgctt ggactcagag ctgtaaacag cacggatgcc atgacggcag
      721 aatggtatcc tttcccgcat gatgttttgc agaagatctc cagtaggatt ctgcaagaag
      781 taccgcaggt aaaccgcgtc gtttacgatg tcagttcaaa gccacctgcg acaatcgagt
      841 gggagtaaag ttcaaacaag tgtagacgcc aatcaggcaa aaccgttgag aagcctcaat
      901 ggaggttttc aggtaggata tgatgtaggt tggaacaaat aatagaacaa gactaaagaa
      961 gacactatca agtacctgtt tcgggcaatt gtgatacggt cgttctgact aaagggtatt
     1021 gaggtagttt ttcgcaagag aaaaggtaaa gatccgttgt agtagttgcg gttgtaggaa
     1081 atgaggctag cttttggtct catttttatt gggaggggtg aaaaaatgga gtttaagctc
     1141 gaatcggtga gtctgcagaa gcaagaaaag acgattctct caagtatcag tttggagatc
     1201 cctgcaggtt cgattttcat tctcataggc ccgtcgggct cggggaagag cagcttactc
     1261 agactgctaa acaggttgga tgcaccgaca agcggcagaa tcctttacaa agaccagcct
     1321 attgaatcct accctgttca ggaactccgc aaagaggtag gcatgctgtt tcaacaccct
     1381 gttatgatca acggaacggt ccgagacaat attgctttag ggcacaagct gcaaaaaaaa
     1441 gaccttcctg aagaaaaggt cttggaactt atcgaacgtg tgggattgga aaaggatctg
     1501 atagacaaag atgcctccgt tttatcggga ggacaaaa
//
LOCUS       DAOSDA010000368         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_55093_length_1534_cov_3.510480, whole genome shotgun sequence.
ACCESSION   DAOSDA010000368 DAOSDA010000000
VERSION     DAOSDA010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55093_length_1534_cov_3.510480"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLW82_07885"
     CDS             <1..420
                     /locus_tag="PLW82_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ47232.1"
                     /translation="KCGTLVRDIEVEDTAVAIVKYKNGAYGVIQGTTSVYPGLDTRFE
                     VHGELGTVIFDDHGFKTWKFLGGEEEVPEVEGYVTSASDPAALSLSGHYIQIDDMAKA
                     VLEDREPMVPGEEARKAVDLILAIYESSKTGKEIILG"
     gene            487..1389
                     /locus_tag="PLW82_07890"
     CDS             487..1389
                     /locus_tag="PLW82_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009677190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ47233.1"
                     /translation="MKELKVGIIGLDTSHVVAFTGLLNDKNHEYHIPGGKVLVAYPGG
                     SPDFELSIGRVKGFTEELQTKYGVEIVDSVEAVAEASDAILLESVDGRVHLEQFKKIV
                     GYGKPIFVDKPFAVQSAHVREMIELARQHNIPLMSSSALRYAEALQEVLADDAKGEII
                     GADFYGPMAIEPTQPGLFWYGIHTVEMLFAALGKGCVQVTATTNDDHDLIVGVWKDGR
                     IGTIRGNRSGNSQFGGVVHRKQGTQFVDVYSHKKPYFASLLEEVMKMFQEGSVPIDLE
                     ESYEVIRFIEAANESRETGKTVKL"
BASE COUNT          406 a          374 c          413 g          341 t
ORIGIN      
        1 aagtgcggca cgttggtccg tgacattgaa gtagaagaca cagcagtcgc cattgtgaag
       61 tacaaaaacg gcgcctacgg cgtaattcag ggcacaacct ccgtatatcc aggtctggac
      121 actcgttttg aggtccacgg ggagctcggc accgtcatct tcgacgacca cggcttcaag
      181 acctggaagt tcctaggtgg cgaagaggaa gtcccagagg tagagggtta cgtgacttca
      241 gcatcagatc ctgcagctct tagcctttct ggacactaca tccagattga cgacatggct
      301 aaagccgtcc tcgaagacag agaaccgatg gttccgggag aggaagccag aaaggccgta
      361 gacctgatct tggcaatcta cgagtcgtca aaaaccggta aggaaatcat tttgggatag
      421 aaggtcctca gggacccttc tatcccaaaa atgcgaaatc tgttttttta gatcggaggt
      481 attcagatga aagagttgaa agtcggcata atcggtttag atacctcgca cgttgtagct
      541 tttacaggcc ttttgaacga caaaaaccac gagtaccaca ttcccggagg taaggtttta
      601 gtagcctatc ccggcggctc accggatttt gagctcagta tcggtagggt aaagggcttt
      661 acagaagaac tgcagaccaa gtacggcgta gagatcgttg acagcgttga agctgttgcc
      721 gaggcttccg atgccatcct tcttgagtct gtagacggca gagtacatct tgagcagttt
      781 aaaaagattg tcggctacgg caagcctatc ttcgtcgaca agccctttgc ggtgcaaagc
      841 gcccacgtcc gcgagatgat cgaacttgca agacagcaca acataccatt aatgtcctca
      901 tctgctctcc gctacgcgga agctcttcag gaagtcctgg cagatgacgc aaaaggcgaa
      961 atcatcggtg ccgacttcta cggtcccatg gccatcgagc caactcagcc cggtcttttc
     1021 tggtacggca tccacacagt agagatgctc tttgcagcac tcggaaaggg atgcgttcag
     1081 gtcaccgcta caaccaacga cgatcacgac ctgatcgtgg gagtttggaa ggatggcaga
     1141 atcggtacca tcaggggcaa ccgctccggg aactcccagt tcggtggcgt ggtccacaga
     1201 aagcagggaa cgcagtttgt ggatgtctac agccacaaga agccctactt cgccagcctc
     1261 ttggaggaag tcatgaagat gttccaggaa ggctctgttc ctatcgatct tgaagaaagc
     1321 tacgaagtta tcagattcat cgaggcagcc aatgagagcc gggaaaccgg aaagacagta
     1381 aaactttgaa aggggatttc aatgcctgat aggtttaaac tgggtatcat tacagacgag
     1441 gtttctcagg attttgatgt ggctttggag cttgcagaca ggttcaatct ggaagcagta
     1501 gagctccgca gcgtctacgg taggcccctc caca
//
LOCUS       DAOSDA010000369         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1843
            NODE_56154_length_1513_cov_3.545267, whole genome shotgun sequence.
ACCESSION   DAOSDA010000369 DAOSDA010000000
VERSION     DAOSDA010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863372
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:24:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,580
            CDSs (total)                      :: 1,562
            Genes (coding)                    :: 1,543
            CDSs (with protein)               :: 1,543
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56154_length_1513_cov_3.545267"
                     /isolate="AMR_MDS_1843"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PLW82_07895"
     CDS             complement(<1..846)
                     /locus_tag="PLW82_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ47234.1"
                     /translation="MSARNKLAIPGCRLNLLGFLKALGFFRSIYLQFDKKVKGYWDED
                     SFVIETSLEKGELIACFAKRFRPLPVLSPLEEDRSGDIYKKLANSSNPMLEHLQHALN
                     AFYSVKDDLSGLTSPFPSTKWLHEVERNLNVLPDVCAPSYKALAMFFHGLGDALRSST
                     GSHLYGNYRFNLMKLKIEENYLASVAKLIDFTSNAPSDGARRLFIDAVFSEPKHVEDP
                     TISHNRFQFNRWDEVVIDFNGNPWDYVFAVYGLVALCKNYPLLAKIPLPFFLKAIGSR
                     WVFGSE"
     gene            complement(1034..>1513)
                     /locus_tag="PLW82_07900"
     CDS             complement(1034..>1513)
                     /locus_tag="PLW82_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOJ47235.1"
                     /translation="NIVLTGDRKSSADSALTCLDLDAVEAELLPEDKLKYVDMLKQTK
                     KPMFIGDGVNDVPVLASAYVGVAMGGIGSDAAIEASDVILVTDEPKKVPLLIKLARAV
                     QGKVAVNIGLALGIKLLVIALSIFGLSGMWQAIVADVGVTLVAILNAASLFTKFKNA"
BASE COUNT          382 a          383 c          374 g          374 t
ORIGIN      
        1 ctcgctgcca aagacccatc tgctgccgat cgctttaagg aaaaacggca gaggaatctt
       61 tgcgagcagc ggatagttct tacacagtgc aaccagtccg tatacggcaa aaacgtagtc
      121 ccaaggattg ccgttaaagt ctatgacgac ttcgtcccag cggttgaact ggaacctgtt
      181 gtgggaaatg gtaggatctt ccacgtgctt gggttccgaa aacaccgcat cgatgaacag
      241 ccgccgcgca ccgtccgacg gtgcattaga ggtaaaatct ataagtttgg ccacagaggc
      301 caggtagttc tcttcgattt tcagcttcat gaggttgaac cggtagttgc cgtacaggtg
      361 agatccggtg gaacttctta aagcatcgcc taaaccgtga aagaacatag ctagggcttt
      421 gtacgaaggt gcacatacgt caggcagcac atttaagttc ctttccactt catgaagcca
      481 cttggtagag gggaaagggg aagtcagccc cgaaaggtcg tctttgaccg aatagaaggc
      541 gtttagggca tgctgcaagt gttcgagcat cggattggac gaatttgcca acttcttgta
      601 tatgtcgcca cttctgtctt cttccagcgg agataacacc ggaaggggtc tgaaacgttt
      661 ggcaaaacag gctatgagct ctcccttttc tagagacgtt tcgattacaa aggagtcttc
      721 gtcccagtac cccttaactt tcttgtcgaa ctggagatag atcgaccgga aaaagcccag
      781 cgctttaaga aagcccagga ggtttaagcg gcatccaggg attgcaagtt tgttacgagc
      841 cgacattgtc tcaccccact tcgcgctact atctatctat tttataatct cttgtttgat
      901 ttcctgcata aactcacact accttttatc ttcacagtca agagagccgg tttgaaaacc
      961 cgcaaaaaaa ggcccaaagg gcgcttttcc ctttgggcgc aaggaaaaat gggacttttg
     1021 tcggaaggct gggttaggcg tttttgaact tggtaaagag cgatgcggca ttcaggatag
     1081 ccaccagggt cacgccgaca tcggctacta tcgcctgcca catgcccgag agtccaaaga
     1141 tggaaagggc gataactaga agcttgatgc ccagcgcaag accgatgttt acggccacct
     1201 taccctgaac agctcgggcc agcttaatta ggagcggcac tttcttaggc tcatccgtca
     1261 caaggatcac gtcgctagct tcgatagctg catcggatcc gatgcctccc atggcaactc
     1321 ccacgtatgc gctagcgaga acaggcacgt cgttaacgcc atcgccgatg aacatgggct
     1381 ttttcgtctg cttgagcata tccacgtact tcagtttatc ttcaggaagc aactctgctt
     1441 ccactgcatc taaatccaaa cacgtgaggg cgctgtctgc actgctcttt ctgtctcctg
     1501 taaggacaat gtt
//
